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Id Subject Object Predicate Lexical cue
T4611 0-9 NN denotes Targeting
T4612 10-19 NN denotes construct
T4613 20-23 IN denotes for
T4614 24-25 DT denotes a
T4615 41-46 NN denotes locus
T4616 26-29 NN denotes p53
T4617 30-34 NN denotes RMCE
T4618 35-40 JJ denotes ready
T4619 34-35 HYPH denotes -
T4620 46-160 sentence denotes Details for plasmid construction are available upon request owing to space limitations mandating a brief outline.
T4621 47-54 NNS denotes Details
T4622 80-83 VBP denotes are
T4623 55-58 IN denotes for
T4624 59-66 NN denotes plasmid
T4625 67-79 NN denotes construction
T4626 84-93 JJ denotes available
T4627 94-98 IN denotes upon
T4628 99-106 NN denotes request
T4629 107-112 VBG denotes owing
T4630 113-115 IN denotes to
T4631 116-121 NN denotes space
T4632 122-133 NNS denotes limitations
T4633 134-143 VBG denotes mandating
T4634 144-145 DT denotes a
T4635 152-159 NN denotes outline
T4636 146-151 JJ denotes brief
T4637 159-160 . denotes .
T4638 160-306 sentence denotes We started from a plasmid L3-1L containing heterologous loxP sites (L3 is the mutant loxP257 recently described (14), 1L is an inverted WT loxP).
T4639 161-163 PRP denotes We
T4640 164-171 VBD denotes started
T4641 172-176 IN denotes from
T4642 177-178 DT denotes a
T4643 190-192 NN denotes 1L
T4644 179-186 NN denotes plasmid
T4645 187-189 NN denotes L3
T4646 189-190 HYPH denotes -
T4647 193-203 VBG denotes containing
T4648 204-216 JJ denotes heterologous
T4649 222-227 NNS denotes sites
T4650 217-221 NN denotes loxP
T4651 228-229 -LRB- denotes (
T4652 282-284 VBZ denotes is
T4653 229-231 NN denotes L3
T4654 232-234 VBZ denotes is
T4655 235-238 DT denotes the
T4656 246-253 NN denotes loxP257
T4657 239-245 JJ denotes mutant
T4658 254-262 RB denotes recently
T4659 263-272 VBN denotes described
T4660 273-274 -LRB- denotes (
T4661 274-276 CD denotes 14
T4662 276-277 -RRB- denotes )
T4663 277-279 , denotes ,
T4664 279-281 NN denotes 1L
T4665 285-287 DT denotes an
T4666 300-304 NN denotes loxP
T4667 288-296 VBN denotes inverted
T4668 297-299 NN denotes WT
T4669 304-305 -RRB- denotes )
T4670 305-306 . denotes .
T4671 306-446 sentence denotes The WT loxP and loxP257 differ in their spacer sequences: the spacer sequence is 5′-ATGTATGC-3′ for WT loxP and 5′-AAGTCTCC-3′ for loxP257.
T4672 307-310 DT denotes The
T4673 314-318 NN denotes loxP
T4674 311-313 NN denotes WT
T4675 331-337 VBP denotes differ
T4676 319-322 CC denotes and
T4677 323-330 NN denotes loxP257
T4678 385-387 VBZ denotes is
T4679 338-340 IN denotes in
T4680 341-346 PRP$ denotes their
T4681 354-363 NNS denotes sequences
T4682 347-353 NN denotes spacer
T4683 363-365 : denotes :
T4684 365-368 DT denotes the
T4685 376-384 NN denotes sequence
T4686 369-375 NN denotes spacer
T4687 388-389 CD denotes 5
T4688 389-390 SYM denotes ′
T4689 390-391 HYPH denotes -
T4690 391-399 JJ denotes ATGTATGC
T4691 399-400 HYPH denotes -
T4692 400-401 CD denotes 3
T4693 401-402 SYM denotes ′
T4694 403-406 IN denotes for
T4695 407-409 NN denotes WT
T4696 410-414 NN denotes loxP
T4697 415-418 CC denotes and
T4698 419-420 CD denotes 5
T4699 422-430 NN denotes AAGTCTCC
T4700 420-421 SYM denotes ′
T4701 421-422 HYPH denotes -
T4702 430-431 HYPH denotes -
T4703 431-432 CD denotes 3
T4704 432-433 SYM denotes ′
T4705 434-437 IN denotes for
T4706 438-445 NN denotes loxP257
T4707 445-446 . denotes .
T4708 446-750 sentence denotes The three mutations in the loxP257 spacer sequence prevent it to recombine with WT loxP, ensuring efficient RMCE in several cell lines: accurate RMCE with these loxP sites occurred with an average frequency of 81% at two loci in CHO cells and an average frequency of 69% at four loci in Hela cells (14).
T4709 447-450 DT denotes The
T4710 457-466 NNS denotes mutations
T4711 451-456 CD denotes three
T4712 498-505 VBP denotes prevent
T4713 467-469 IN denotes in
T4714 470-473 DT denotes the
T4715 489-497 NN denotes sequence
T4716 474-481 NN denotes loxP257
T4717 482-488 NN denotes spacer
T4718 619-627 VBD denotes occurred
T4719 506-508 PRP denotes it
T4720 512-521 VB denotes recombine
T4721 509-511 TO denotes to
T4722 522-526 IN denotes with
T4723 527-529 NN denotes WT
T4724 530-534 NN denotes loxP
T4725 534-536 , denotes ,
T4726 536-544 VBG denotes ensuring
T4727 545-554 JJ denotes efficient
T4728 555-559 NN denotes RMCE
T4729 560-562 IN denotes in
T4730 563-570 JJ denotes several
T4731 576-581 NNS denotes lines
T4732 571-575 NN denotes cell
T4733 581-583 : denotes :
T4734 583-591 JJ denotes accurate
T4735 592-596 NN denotes RMCE
T4736 597-601 IN denotes with
T4737 602-607 DT denotes these
T4738 613-618 NNS denotes sites
T4739 608-612 NN denotes loxP
T4740 628-632 IN denotes with
T4741 633-635 DT denotes an
T4742 644-653 NN denotes frequency
T4743 636-643 JJ denotes average
T4744 654-656 IN denotes of
T4745 657-659 CD denotes 81
T4746 659-660 NN denotes %
T4747 661-663 IN denotes at
T4748 664-667 CD denotes two
T4749 668-672 NNS denotes loci
T4750 673-675 IN denotes in
T4751 676-679 NN denotes CHO
T4752 680-685 NNS denotes cells
T4753 686-689 CC denotes and
T4754 690-692 DT denotes an
T4755 701-710 NN denotes frequency
T4756 693-700 JJ denotes average
T4757 711-713 IN denotes of
T4758 714-716 CD denotes 69
T4759 716-717 NN denotes %
T4760 718-720 IN denotes at
T4761 721-725 CD denotes four
T4762 726-730 NNS denotes loci
T4763 731-733 IN denotes in
T4764 734-738 NN denotes Hela
T4765 739-744 NNS denotes cells
T4766 745-746 -LRB- denotes (
T4767 746-748 CD denotes 14
T4768 748-749 -RRB- denotes )
T4769 749-750 . denotes .
T4770 750-874 sentence denotes The L3-1L plasmid was first modified to include a ClaI and a FseI site between the LoxP sites, leading to plasmid L3-CF-1L.
T4771 751-754 DT denotes The
T4772 761-768 NN denotes plasmid
T4773 755-757 NN denotes L3
T4774 758-760 NN denotes 1L
T4775 757-758 HYPH denotes -
T4776 779-787 VBN denotes modified
T4777 769-772 VBD denotes was
T4778 773-778 RB denotes first
T4779 788-790 TO denotes to
T4780 791-798 VB denotes include
T4781 799-800 DT denotes a
T4782 801-805 NN denotes ClaI
T4783 806-809 CC denotes and
T4784 810-811 DT denotes a
T4785 817-821 NN denotes site
T4786 812-816 NN denotes FseI
T4787 822-829 IN denotes between
T4788 830-833 DT denotes the
T4789 839-844 NNS denotes sites
T4790 834-838 NN denotes LoxP
T4791 844-846 , denotes ,
T4792 846-853 VBG denotes leading
T4793 854-856 IN denotes to
T4794 857-864 NN denotes plasmid
T4795 871-873 NN denotes 1L
T4796 865-867 NN denotes L3
T4797 867-868 HYPH denotes -
T4798 868-870 NN denotes CF
T4799 870-871 HYPH denotes -
T4800 873-874 . denotes .
T4801 874-1136 sentence denotes We next modified a puroΔTK plasmid (YTC37, a kind gift from A. Bradley) by using oligonucleotides to destroy a NotI site downstream of the puroΔTK gene and introduce a NotI site upstream, and a FseI site downstream of the gene (leading to plasmid CN-PuroΔTK-F).
T4802 875-877 PRP denotes We
T4803 883-891 VBD denotes modified
T4804 878-882 RB denotes next
T4805 892-893 DT denotes a
T4806 902-909 NN denotes plasmid
T4807 894-901 NN denotes puroΔTK
T4808 910-911 -LRB- denotes (
T4809 911-916 NN denotes YTC37
T4810 916-918 , denotes ,
T4811 918-919 DT denotes a
T4812 925-929 NN denotes gift
T4813 920-924 NN denotes kind
T4814 930-934 IN denotes from
T4815 935-937 NNP denotes A.
T4816 938-945 NNP denotes Bradley
T4817 945-946 -RRB- denotes )
T4818 947-949 IN denotes by
T4819 950-955 VBG denotes using
T4820 956-972 NNS denotes oligonucleotides
T4821 973-975 TO denotes to
T4822 976-983 VB denotes destroy
T4823 984-985 DT denotes a
T4824 991-995 NN denotes site
T4825 986-990 NN denotes NotI
T4826 996-1006 RB denotes downstream
T4827 1007-1009 IN denotes of
T4828 1010-1013 DT denotes the
T4829 1022-1026 NN denotes gene
T4830 1014-1021 NN denotes puroΔTK
T4831 1027-1030 CC denotes and
T4832 1031-1040 VB denotes introduce
T4833 1041-1042 DT denotes a
T4834 1048-1052 NN denotes site
T4835 1043-1047 NN denotes NotI
T4836 1053-1061 RB denotes upstream
T4837 1061-1063 , denotes ,
T4838 1063-1066 CC denotes and
T4839 1067-1068 DT denotes a
T4840 1074-1078 NN denotes site
T4841 1069-1073 NN denotes FseI
T4842 1079-1089 RB denotes downstream
T4843 1090-1092 IN denotes of
T4844 1093-1096 DT denotes the
T4845 1097-1101 NN denotes gene
T4846 1102-1103 -LRB- denotes (
T4847 1103-1110 VBG denotes leading
T4848 1111-1113 IN denotes to
T4849 1114-1121 NN denotes plasmid
T4850 1133-1134 NN denotes F
T4851 1122-1124 NN denotes CN
T4852 1124-1125 HYPH denotes -
T4853 1125-1132 NN denotes PuroΔTK
T4854 1132-1133 HYPH denotes -
T4855 1134-1135 -RRB- denotes )
T4856 1135-1136 . denotes .
T4857 1136-1384 sentence denotes Next, a PmlI-MfeI 6.3 kb fragment from Trp53 was subcloned in a modified pBluescript KSII+ (pBS, Stratagene), and the resulting plasmid was digested with SwaI to introduce an EagI site, leading to p53PmlEag, a plasmid containing exons 2–11 of p53.
T4858 1137-1141 RB denotes Next
T4859 1186-1195 VBN denotes subcloned
T4860 1141-1143 , denotes ,
T4861 1143-1144 DT denotes a
T4862 1162-1170 NN denotes fragment
T4863 1145-1149 NN denotes PmlI
T4864 1150-1154 NN denotes MfeI
T4865 1149-1150 HYPH denotes -
T4866 1155-1158 CD denotes 6.3
T4867 1159-1161 NN denotes kb
T4868 1171-1175 IN denotes from
T4869 1176-1181 NN denotes Trp53
T4870 1182-1185 VBD denotes was
T4871 1196-1198 IN denotes in
T4872 1199-1200 DT denotes a
T4873 1222-1227 NN denotes KSII+
T4874 1201-1209 VBN denotes modified
T4875 1210-1221 NN denotes pBluescript
T4876 1228-1229 -LRB- denotes (
T4877 1229-1232 NN denotes pBS
T4878 1232-1234 , denotes ,
T4879 1234-1244 NNP denotes Stratagene
T4880 1244-1245 -RRB- denotes )
T4881 1245-1247 , denotes ,
T4882 1247-1250 CC denotes and
T4883 1251-1254 DT denotes the
T4884 1265-1272 NN denotes plasmid
T4885 1255-1264 VBG denotes resulting
T4886 1277-1285 VBN denotes digested
T4887 1273-1276 VBD denotes was
T4888 1286-1290 IN denotes with
T4889 1291-1295 NN denotes SwaI
T4890 1296-1298 TO denotes to
T4891 1299-1308 VB denotes introduce
T4892 1309-1311 DT denotes an
T4893 1317-1321 NN denotes site
T4894 1312-1316 NN denotes EagI
T4895 1321-1323 , denotes ,
T4896 1323-1330 VBG denotes leading
T4897 1331-1333 IN denotes to
T4898 1334-1343 NN denotes p53PmlEag
T4899 1343-1345 , denotes ,
T4900 1345-1346 DT denotes a
T4901 1347-1354 NN denotes plasmid
T4902 1355-1365 VBG denotes containing
T4903 1366-1371 NNS denotes exons
T4904 1374-1376 CD denotes 11
T4905 1372-1373 CD denotes 2
T4906 1373-1374 HYPH denotes –
T4907 1377-1379 IN denotes of
T4908 1380-1383 NN denotes p53
T4909 1383-1384 . denotes .
T4910 1384-1629 sentence denotes We then inserted a 5.5 kb ClaI-EagI fragment from p53PmlEag in plasmid CN-PuroΔTK-F digested by ClaI and NotI, and inserted the resulting fragment between the loxP sites of L3-CF-1L by ClaI and FseI digestion, leading to L3-p53PmlEagPuroΔTK-1L.
T4911 1385-1387 PRP denotes We
T4912 1393-1401 VBD denotes inserted
T4913 1388-1392 RB denotes then
T4914 1402-1403 DT denotes a
T4915 1421-1429 NN denotes fragment
T4916 1404-1407 CD denotes 5.5
T4917 1408-1410 NN denotes kb
T4918 1411-1415 NN denotes ClaI
T4919 1416-1420 NN denotes EagI
T4920 1415-1416 HYPH denotes -
T4921 1430-1434 IN denotes from
T4922 1435-1444 NN denotes p53PmlEag
T4923 1445-1447 IN denotes in
T4924 1448-1455 NN denotes plasmid
T4925 1467-1468 NN denotes F
T4926 1456-1458 NN denotes CN
T4927 1458-1459 HYPH denotes -
T4928 1459-1466 NN denotes PuroΔTK
T4929 1466-1467 HYPH denotes -
T4930 1469-1477 VBN denotes digested
T4931 1478-1480 IN denotes by
T4932 1481-1485 NN denotes ClaI
T4933 1486-1489 CC denotes and
T4934 1490-1494 NN denotes NotI
T4935 1494-1496 , denotes ,
T4936 1496-1499 CC denotes and
T4937 1500-1508 VBD denotes inserted
T4938 1509-1512 DT denotes the
T4939 1523-1531 NN denotes fragment
T4940 1513-1522 VBG denotes resulting
T4941 1532-1539 IN denotes between
T4942 1540-1543 DT denotes the
T4943 1549-1554 NNS denotes sites
T4944 1544-1548 NN denotes loxP
T4945 1555-1557 IN denotes of
T4946 1558-1560 NN denotes L3
T4947 1564-1566 NN denotes 1L
T4948 1560-1561 HYPH denotes -
T4949 1561-1563 NN denotes CF
T4950 1563-1564 HYPH denotes -
T4951 1567-1569 IN denotes by
T4952 1570-1574 NN denotes ClaI
T4953 1584-1593 NN denotes digestion
T4954 1575-1578 CC denotes and
T4955 1579-1583 NN denotes FseI
T4956 1593-1595 , denotes ,
T4957 1595-1602 VBG denotes leading
T4958 1603-1605 IN denotes to
T4959 1606-1608 NN denotes L3
T4960 1626-1628 NN denotes 1L
T4961 1608-1609 HYPH denotes -
T4962 1609-1625 NN denotes p53PmlEagPuroΔTK
T4963 1625-1626 HYPH denotes -
T4964 1628-1629 . denotes .
T4965 1629-2219 sentence denotes We next engineered a plasmid containing the region for 3′-homology downstream of the p53 gene and the DTA gene in two steps: (i) we performed a three-way ligation between a modified pBS digested by HindIII and NotI, a HindIII-EcoRI fragment from Trp53 for 3′homology and an EcoRI-NotI fragment containing the DTA gene, from plasmid pgkdtabpa (kind gift of P. Soriano), leading to plasmid 3′ + DTA and (ii) because the Bsu36I-EcoRI region downstream of p53 contains repetitive sequences (F. Toledo and G. M. Wahl, unpublished data), we later deleted this region, to obtain plasmid 3′ + DTA.
T4966 1630-1632 PRP denotes We
T4967 1638-1648 VBD denotes engineered
T4968 1633-1637 RB denotes next
T4969 1762-1771 VBD denotes performed
T4970 1649-1650 DT denotes a
T4971 1651-1658 NN denotes plasmid
T4972 1659-1669 VBG denotes containing
T4973 1670-1673 DT denotes the
T4974 1674-1680 NN denotes region
T4975 1681-1684 IN denotes for
T4976 1685-1686 CD denotes 3
T4977 1686-1687 SYM denotes ′
T4978 1687-1688 SYM denotes -
T4979 1688-1696 JJ denotes homology
T4980 1697-1707 RB denotes downstream
T4981 1708-1710 IN denotes of
T4982 1711-1714 DT denotes the
T4983 1719-1723 NN denotes gene
T4984 1715-1718 NN denotes p53
T4985 1724-1727 CC denotes and
T4986 1728-1731 DT denotes the
T4987 1736-1740 NN denotes gene
T4988 1732-1735 NN denotes DTA
T4989 1741-1743 IN denotes in
T4990 1744-1747 CD denotes two
T4991 1748-1753 NNS denotes steps
T4992 1753-1755 : denotes :
T4993 1755-1756 -LRB- denotes (
T4994 1756-1757 LS denotes i
T4995 1757-1758 -RRB- denotes )
T4996 1759-1761 PRP denotes we
T4997 1772-1773 DT denotes a
T4998 1784-1792 NN denotes ligation
T4999 1774-1779 CD denotes three
T5000 1780-1783 NN denotes way
T5001 1779-1780 HYPH denotes -
T5002 1793-1800 IN denotes between
T5003 1801-1802 DT denotes a
T5004 1812-1815 NN denotes pBS
T5005 1803-1811 VBN denotes modified
T5006 1816-1824 VBN denotes digested
T5007 1825-1827 IN denotes by
T5008 1828-1835 NN denotes HindIII
T5009 1836-1839 CC denotes and
T5010 1840-1844 NN denotes NotI
T5011 1844-1846 , denotes ,
T5012 1846-1847 DT denotes a
T5013 1862-1870 NN denotes fragment
T5014 1848-1855 NN denotes HindIII
T5015 1856-1861 NN denotes EcoRI
T5016 1855-1856 HYPH denotes -
T5017 1871-1875 IN denotes from
T5018 1876-1881 NN denotes Trp53
T5019 1882-1885 IN denotes for
T5020 1886-1887 CD denotes 3
T5021 1888-1896 NN denotes homology
T5022 1887-1888 SYM denotes ′
T5023 1897-1900 CC denotes and
T5024 1901-1903 DT denotes an
T5025 1915-1923 NN denotes fragment
T5026 1904-1909 NN denotes EcoRI
T5027 1910-1914 NN denotes NotI
T5028 1909-1910 HYPH denotes -
T5029 1924-1934 VBG denotes containing
T5030 1935-1938 DT denotes the
T5031 1943-1947 NN denotes gene
T5032 1939-1942 NN denotes DTA
T5033 1947-1949 , denotes ,
T5034 1949-1953 IN denotes from
T5035 1954-1961 NN denotes plasmid
T5036 1962-1971 NN denotes pgkdtabpa
T5037 1972-1973 -LRB- denotes (
T5038 1973-1977 JJ denotes kind
T5039 1978-1982 NN denotes gift
T5040 1983-1985 IN denotes of
T5041 1986-1988 NNP denotes P.
T5042 1989-1996 NNP denotes Soriano
T5043 1996-1997 -RRB- denotes )
T5044 1997-1999 , denotes ,
T5045 1999-2006 VBG denotes leading
T5046 2007-2009 IN denotes to
T5047 2010-2017 NN denotes plasmid
T5048 2023-2026 NN denotes DTA
T5049 2018-2019 CD denotes 3
T5050 2019-2020 SYM denotes ′
T5051 2021-2022 SYM denotes +
T5052 2027-2030 CC denotes and
T5053 2031-2032 -LRB- denotes (
T5054 2032-2034 LS denotes ii
T5055 2171-2178 VBD denotes deleted
T5056 2034-2035 -RRB- denotes )
T5057 2036-2043 IN denotes because
T5058 2086-2094 VBZ denotes contains
T5059 2044-2047 DT denotes the
T5060 2061-2067 NN denotes region
T5061 2048-2054 NN denotes Bsu36I
T5062 2055-2060 NN denotes EcoRI
T5063 2054-2055 HYPH denotes -
T5064 2068-2078 RB denotes downstream
T5065 2079-2081 IN denotes of
T5066 2082-2085 NN denotes p53
T5067 2095-2105 JJ denotes repetitive
T5068 2106-2115 NNS denotes sequences
T5069 2116-2117 -LRB- denotes (
T5070 2117-2119 NNP denotes F.
T5071 2120-2126 NNP denotes Toledo
T5072 2127-2130 CC denotes and
T5073 2131-2133 NNP denotes G.
T5074 2134-2136 NNP denotes M.
T5075 2137-2141 NNP denotes Wahl
T5076 2141-2143 , denotes ,
T5077 2143-2154 JJ denotes unpublished
T5078 2155-2159 NNS denotes data
T5079 2159-2160 -RRB- denotes )
T5080 2160-2162 , denotes ,
T5081 2162-2164 PRP denotes we
T5082 2165-2170 RB denotes later
T5083 2179-2183 DT denotes this
T5084 2184-2190 NN denotes region
T5085 2190-2192 , denotes ,
T5086 2192-2194 TO denotes to
T5087 2195-2201 VB denotes obtain
T5088 2202-2209 NN denotes plasmid
T5089 2215-2218 NN denotes DTA
T5090 2210-2211 CD denotes 3
T5091 2211-2212 SYM denotes ′
T5092 2213-2214 SYM denotes +
T5093 2218-2219 . denotes .
T5094 2219-2342 sentence denotes The 5′ homology consists of a 3.4 kb-long BamHI-PmlI fragment from intron 1 of p53 cloned in a modified pBS (plasmid p5′).
T5095 2220-2223 DT denotes The
T5096 2227-2235 NN denotes homology
T5097 2224-2225 CD denotes 5
T5098 2225-2226 SYM denotes ′
T5099 2236-2244 VBZ denotes consists
T5100 2245-2247 IN denotes of
T5101 2248-2249 DT denotes a
T5102 2273-2281 NN denotes fragment
T5103 2250-2253 CD denotes 3.4
T5104 2254-2256 NN denotes kb
T5105 2257-2261 JJ denotes long
T5106 2256-2257 HYPH denotes -
T5107 2262-2267 NN denotes BamHI
T5108 2268-2272 NN denotes PmlI
T5109 2267-2268 HYPH denotes -
T5110 2282-2286 IN denotes from
T5111 2287-2293 NN denotes intron
T5112 2294-2295 CD denotes 1
T5113 2296-2298 IN denotes of
T5114 2299-2302 NN denotes p53
T5115 2303-2309 VBN denotes cloned
T5116 2310-2312 IN denotes in
T5117 2313-2314 DT denotes a
T5118 2324-2327 NN denotes pBS
T5119 2315-2323 VBN denotes modified
T5120 2328-2329 -LRB- denotes (
T5121 2329-2336 NN denotes plasmid
T5122 2337-2339 NN denotes p5
T5123 2339-2340 SYM denotes ′
T5124 2340-2341 -RRB- denotes )
T5125 2341-2342 . denotes .
T5126 2342-2483 sentence denotes Finally, appropriate fragments from plasmids p5′, L3-p53PmlEagPuroΔTK-1L, and 3′ + DTA were assembled in a modified pSP72 plasmid (Promega).
T5127 2343-2350 RB denotes Finally
T5128 2435-2444 VBN denotes assembled
T5129 2350-2352 , denotes ,
T5130 2352-2363 JJ denotes appropriate
T5131 2364-2373 NNS denotes fragments
T5132 2374-2378 IN denotes from
T5133 2379-2387 NNS denotes plasmids
T5134 2388-2390 NN denotes p5
T5135 2390-2391 SYM denotes ′
T5136 2391-2393 , denotes ,
T5137 2393-2395 NN denotes L3
T5138 2413-2415 NN denotes 1L
T5139 2395-2396 HYPH denotes -
T5140 2396-2412 NN denotes p53PmlEagPuroΔTK
T5141 2412-2413 HYPH denotes -
T5142 2415-2417 , denotes ,
T5143 2417-2420 CC denotes and
T5144 2421-2422 CD denotes 3
T5145 2426-2429 NN denotes DTA
T5146 2422-2423 SYM denotes ′
T5147 2424-2425 SYM denotes +
T5148 2430-2434 VBD denotes were
T5149 2445-2447 IN denotes in
T5150 2448-2449 DT denotes a
T5151 2465-2472 NN denotes plasmid
T5152 2450-2458 VBN denotes modified
T5153 2459-2464 NN denotes pSP72
T5154 2473-2474 -LRB- denotes (
T5155 2474-2481 NNP denotes Promega
T5156 2481-2482 -RRB- denotes )
T5157 2482-2483 . denotes .
T5158 2483-2729 sentence denotes Plasmid Flox, the resulting targeting construct, was verified by restriction analysis, then sequenced using 30 primers chosen to precisely verify all p53 coding sequences, all exon–intron junctions and the sequences at and around the loxP sites.
T5159 2484-2491 NN denotes Plasmid
T5160 2492-2496 NN denotes Flox
T5161 2537-2545 VBN denotes verified
T5162 2496-2498 , denotes ,
T5163 2498-2501 DT denotes the
T5164 2522-2531 NN denotes construct
T5165 2502-2511 VBG denotes resulting
T5166 2512-2521 NN denotes targeting
T5167 2531-2533 , denotes ,
T5168 2533-2536 VBD denotes was
T5169 2546-2548 IN denotes by
T5170 2549-2560 NN denotes restriction
T5171 2561-2569 NN denotes analysis
T5172 2569-2571 , denotes ,
T5173 2571-2575 RB denotes then
T5174 2576-2585 VBN denotes sequenced
T5175 2586-2591 VBG denotes using
T5176 2592-2594 CD denotes 30
T5177 2595-2602 NNS denotes primers
T5178 2603-2609 VBN denotes chosen
T5179 2610-2612 TO denotes to
T5180 2623-2629 VB denotes verify
T5181 2613-2622 RB denotes precisely
T5182 2630-2633 DT denotes all
T5183 2645-2654 NNS denotes sequences
T5184 2634-2637 NN denotes p53
T5185 2638-2644 NN denotes coding
T5186 2654-2656 , denotes ,
T5187 2656-2659 DT denotes all
T5188 2672-2681 NNS denotes junctions
T5189 2660-2664 NN denotes exon
T5190 2665-2671 NN denotes intron
T5191 2664-2665 HYPH denotes –
T5192 2682-2685 CC denotes and
T5193 2686-2689 DT denotes the
T5194 2690-2699 NNS denotes sequences
T5195 2700-2702 IN denotes at
T5196 2703-2706 CC denotes and
T5197 2707-2713 IN denotes around
T5198 2714-2717 DT denotes the
T5199 2723-2728 NNS denotes sites
T5200 2718-2722 NN denotes loxP
T5201 2728-2729 . denotes .
R1050 T4611 T4612 compound Targeting,construct
R1051 T4613 T4612 prep for,construct
R1052 T4614 T4615 det a,locus
R1053 T4615 T4613 pobj locus,for
R1054 T4616 T4615 nmod p53,locus
R1055 T4617 T4618 npadvmod RMCE,ready
R1056 T4618 T4615 amod ready,locus
R1057 T4619 T4618 punct -,ready
R1058 T4621 T4622 nsubj Details,are
R1059 T4623 T4621 prep for,Details
R1060 T4624 T4625 compound plasmid,construction
R1061 T4625 T4623 pobj construction,for
R1062 T4626 T4622 acomp available,are
R1063 T4627 T4622 prep upon,are
R1064 T4628 T4627 pobj request,upon
R1065 T4629 T4622 prep owing,are
R1066 T4630 T4629 prep to,owing
R1067 T4631 T4632 compound space,limitations
R1068 T4632 T4630 pobj limitations,to
R1069 T4633 T4632 acl mandating,limitations
R1070 T4634 T4635 det a,outline
R1071 T4635 T4633 dobj outline,mandating
R1072 T4636 T4635 amod brief,outline
R1073 T4637 T4622 punct .,are
R1074 T4639 T4640 nsubj We,started
R1075 T4641 T4640 prep from,started
R1076 T4642 T4643 det a,1L
R1077 T4643 T4641 pobj 1L,from
R1078 T4644 T4643 compound plasmid,1L
R1079 T4645 T4643 compound L3,1L
R1080 T4646 T4643 punct -,1L
R1081 T4647 T4643 acl containing,1L
R1082 T4648 T4649 amod heterologous,sites
R1083 T4649 T4647 dobj sites,containing
R1084 T4650 T4649 compound loxP,sites
R1085 T4651 T4652 punct (,is
R1086 T4652 T4640 parataxis is,started
R1087 T4653 T4654 nsubj L3,is
R1088 T4654 T4652 ccomp is,is
R1089 T4655 T4656 det the,loxP257
R1090 T4656 T4654 attr loxP257,is
R1091 T4657 T4656 amod mutant,loxP257
R1092 T4658 T4659 advmod recently,described
R1093 T4659 T4656 acl described,loxP257
R1094 T4660 T4661 punct (,14
R1095 T4661 T4654 parataxis 14,is
R1096 T4662 T4661 punct ),14
R1097 T4663 T4652 punct ", ",is
R1098 T4664 T4652 nsubj 1L,is
R1099 T4665 T4666 det an,loxP
R1100 T4666 T4652 attr loxP,is
R1101 T4667 T4666 amod inverted,loxP
R1102 T4668 T4666 compound WT,loxP
R1103 T4669 T4652 punct ),is
R1104 T4670 T4640 punct .,started
R1105 T4672 T4673 det The,loxP
R1106 T4673 T4675 nsubj loxP,differ
R1107 T4674 T4673 compound WT,loxP
R1108 T4675 T4678 ccomp differ,is
R1109 T4676 T4673 cc and,loxP
R1110 T4677 T4673 conj loxP257,loxP
R1111 T4679 T4675 prep in,differ
R1112 T4680 T4681 poss their,sequences
R1113 T4681 T4679 pobj sequences,in
R1114 T4682 T4681 compound spacer,sequences
R1115 T4683 T4678 punct : ,is
R1116 T4684 T4685 det the,sequence
R1117 T4685 T4678 nsubj sequence,is
R1118 T4686 T4685 compound spacer,sequence
R1119 T4687 T4678 attr 5,is
R1120 T4688 T4687 punct ′,5
R1121 T4689 T4687 punct -,5
R1122 T4690 T4687 amod ATGTATGC,5
R1123 T4691 T4687 punct -,5
R1124 T4692 T4687 nummod 3,5
R1125 T4693 T4687 punct ′,5
R1126 T4694 T4687 prep for,5
R1127 T4695 T4696 compound WT,loxP
R1128 T4696 T4694 pobj loxP,for
R1129 T4697 T4687 cc and,5
R1130 T4698 T4699 nummod 5,AAGTCTCC
R1131 T4699 T4687 conj AAGTCTCC,5
R1132 T4700 T4698 punct ′,5
R1133 T4701 T4699 punct -,AAGTCTCC
R1134 T4702 T4699 punct -,AAGTCTCC
R1135 T4703 T4699 nummod 3,AAGTCTCC
R1136 T4704 T4699 punct ′,AAGTCTCC
R1137 T4705 T4699 prep for,AAGTCTCC
R1138 T4706 T4705 pobj loxP257,for
R1139 T4707 T4678 punct .,is
R1140 T4709 T4710 det The,mutations
R1141 T4710 T4712 nsubj mutations,prevent
R1142 T4711 T4710 nummod three,mutations
R1143 T4712 T4718 ccomp prevent,occurred
R1144 T4713 T4710 prep in,mutations
R1145 T4714 T4715 det the,sequence
R1146 T4715 T4713 pobj sequence,in
R1147 T4716 T4715 compound loxP257,sequence
R1148 T4717 T4715 compound spacer,sequence
R1149 T4719 T4720 nsubj it,recombine
R1150 T4720 T4712 ccomp recombine,prevent
R1151 T4721 T4720 aux to,recombine
R1152 T4722 T4720 prep with,recombine
R1153 T4723 T4724 compound WT,loxP
R1154 T4724 T4722 pobj loxP,with
R1155 T4725 T4712 punct ", ",prevent
R1156 T4726 T4712 advcl ensuring,prevent
R1157 T4727 T4728 amod efficient,RMCE
R1158 T4728 T4726 dobj RMCE,ensuring
R1159 T4729 T4726 prep in,ensuring
R1160 T4730 T4731 amod several,lines
R1161 T4731 T4729 pobj lines,in
R1162 T4732 T4731 compound cell,lines
R1163 T4733 T4718 punct : ,occurred
R1164 T4734 T4735 amod accurate,RMCE
R1165 T4735 T4718 nsubj RMCE,occurred
R1166 T4736 T4735 prep with,RMCE
R1167 T4737 T4738 det these,sites
R1168 T4738 T4736 pobj sites,with
R1169 T4739 T4738 compound loxP,sites
R1170 T4740 T4718 prep with,occurred
R1171 T4741 T4742 det an,frequency
R1172 T4742 T4740 pobj frequency,with
R1173 T4743 T4742 amod average,frequency
R1174 T4744 T4742 prep of,frequency
R1175 T4745 T4746 nummod 81,%
R1176 T4746 T4744 pobj %,of
R1177 T4747 T4742 prep at,frequency
R1178 T4748 T4749 nummod two,loci
R1179 T4749 T4747 pobj loci,at
R1180 T4750 T4749 prep in,loci
R1181 T4751 T4752 compound CHO,cells
R1182 T4752 T4750 pobj cells,in
R1183 T4753 T4742 cc and,frequency
R1184 T4754 T4755 det an,frequency
R1185 T4755 T4742 conj frequency,frequency
R1186 T4756 T4755 amod average,frequency
R1187 T4757 T4755 prep of,frequency
R1188 T4758 T4759 nummod 69,%
R1189 T4759 T4757 pobj %,of
R1190 T4760 T4755 prep at,frequency
R1191 T4761 T4762 nummod four,loci
R1192 T4762 T4760 pobj loci,at
R1193 T4763 T4762 prep in,loci
R1194 T4764 T4765 compound Hela,cells
R1195 T4765 T4763 pobj cells,in
R1196 T4766 T4767 punct (,14
R1197 T4767 T4718 parataxis 14,occurred
R1198 T4768 T4767 punct ),14
R1199 T4769 T4718 punct .,occurred
R1200 T4771 T4772 det The,plasmid
R1201 T4772 T4776 nsubjpass plasmid,modified
R1202 T4773 T4774 compound L3,1L
R1203 T4774 T4772 compound 1L,plasmid
R1204 T4775 T4774 punct -,1L
R1205 T4777 T4776 auxpass was,modified
R1206 T4778 T4776 advmod first,modified
R1207 T4779 T4780 aux to,include
R1208 T4780 T4776 advcl include,modified
R1209 T4781 T4782 det a,ClaI
R1210 T4782 T4780 dobj ClaI,include
R1211 T4783 T4782 cc and,ClaI
R1212 T4784 T4785 det a,site
R1213 T4785 T4782 conj site,ClaI
R1214 T4786 T4785 compound FseI,site
R1215 T4787 T4780 prep between,include
R1216 T4788 T4789 det the,sites
R1217 T4789 T4787 pobj sites,between
R1218 T4790 T4789 compound LoxP,sites
R1219 T4791 T4776 punct ", ",modified
R1220 T4792 T4776 advcl leading,modified
R1221 T4793 T4792 prep to,leading
R1222 T4794 T4795 compound plasmid,1L
R1223 T4795 T4793 pobj 1L,to
R1224 T4796 T4795 compound L3,1L
R1225 T4797 T4795 punct -,1L
R1226 T4798 T4795 compound CF,1L
R1227 T4799 T4795 punct -,1L
R1228 T4800 T4776 punct .,modified
R1229 T4802 T4803 nsubj We,modified
R1230 T4804 T4803 advmod next,modified
R1231 T4805 T4806 det a,plasmid
R1232 T4806 T4803 dobj plasmid,modified
R1233 T4807 T4806 compound puroΔTK,plasmid
R1234 T4808 T4806 punct (,plasmid
R1235 T4809 T4806 appos YTC37,plasmid
R1236 T4810 T4809 punct ", ",YTC37
R1237 T4811 T4812 det a,gift
R1238 T4812 T4809 appos gift,YTC37
R1239 T4813 T4812 compound kind,gift
R1240 T4814 T4812 prep from,gift
R1241 T4815 T4816 compound A.,Bradley
R1242 T4816 T4814 pobj Bradley,from
R1243 T4817 T4803 punct ),modified
R1244 T4818 T4803 prep by,modified
R1245 T4819 T4818 pcomp using,by
R1246 T4820 T4819 dobj oligonucleotides,using
R1247 T4821 T4822 aux to,destroy
R1248 T4822 T4819 advcl destroy,using
R1249 T4823 T4824 det a,site
R1250 T4824 T4822 dobj site,destroy
R1251 T4825 T4824 compound NotI,site
R1252 T4826 T4824 advmod downstream,site
R1253 T4827 T4826 prep of,downstream
R1254 T4828 T4829 det the,gene
R1255 T4829 T4827 pobj gene,of
R1256 T4830 T4829 compound puroΔTK,gene
R1257 T4831 T4822 cc and,destroy
R1258 T4832 T4822 conj introduce,destroy
R1259 T4833 T4834 det a,site
R1260 T4834 T4832 dobj site,introduce
R1261 T4835 T4834 compound NotI,site
R1262 T4836 T4832 advmod upstream,introduce
R1263 T4837 T4832 punct ", ",introduce
R1264 T4838 T4832 cc and,introduce
R1265 T4839 T4840 det a,site
R1266 T4840 T4832 conj site,introduce
R1267 T4841 T4840 compound FseI,site
R1268 T4842 T4840 advmod downstream,site
R1269 T4843 T4842 prep of,downstream
R1270 T4844 T4845 det the,gene
R1271 T4845 T4843 pobj gene,of
R1272 T4846 T4847 punct (,leading
R1273 T4847 T4840 parataxis leading,site
R1274 T4848 T4847 prep to,leading
R1275 T4849 T4850 compound plasmid,F
R1276 T4850 T4848 pobj F,to
R1277 T4851 T4850 compound CN,F
R1278 T4852 T4850 punct -,F
R1279 T4853 T4850 compound PuroΔTK,F
R1280 T4854 T4850 punct -,F
R1281 T4855 T4847 punct ),leading
R1282 T4856 T4803 punct .,modified
R1283 T4858 T4859 advmod Next,subcloned
R1284 T4860 T4859 punct ", ",subcloned
R1285 T4861 T4862 det a,fragment
R1286 T4862 T4859 nsubjpass fragment,subcloned
R1287 T4863 T4864 nmod PmlI,MfeI
R1288 T4864 T4862 nmod MfeI,fragment
R1289 T4865 T4864 punct -,MfeI
R1290 T4866 T4867 nummod 6.3,kb
R1291 T4867 T4862 compound kb,fragment
R1292 T4868 T4862 prep from,fragment
R1293 T4869 T4868 pobj Trp53,from
R1294 T4870 T4859 auxpass was,subcloned
R1295 T4871 T4859 prep in,subcloned
R1296 T4872 T4873 det a,KSII+
R1297 T4873 T4871 pobj KSII+,in
R1298 T4874 T4873 amod modified,KSII+
R1299 T4875 T4873 compound pBluescript,KSII+
R1300 T4876 T4877 punct (,pBS
R1301 T4877 T4873 parataxis pBS,KSII+
R1302 T4878 T4877 punct ", ",pBS
R1303 T4879 T4877 npadvmod Stratagene,pBS
R1304 T4880 T4877 punct ),pBS
R1305 T4881 T4859 punct ", ",subcloned
R1306 T4882 T4859 cc and,subcloned
R1307 T4883 T4884 det the,plasmid
R1308 T4884 T4886 nsubjpass plasmid,digested
R1309 T4885 T4884 amod resulting,plasmid
R1310 T4886 T4859 conj digested,subcloned
R1311 T4887 T4886 auxpass was,digested
R1312 T4888 T4886 prep with,digested
R1313 T4889 T4888 pobj SwaI,with
R1314 T4890 T4891 aux to,introduce
R1315 T4891 T4886 advcl introduce,digested
R1316 T4892 T4893 det an,site
R1317 T4893 T4891 dobj site,introduce
R1318 T4894 T4893 compound EagI,site
R1319 T4895 T4891 punct ", ",introduce
R1320 T4896 T4891 advcl leading,introduce
R1321 T4897 T4896 prep to,leading
R1322 T4898 T4897 pobj p53PmlEag,to
R1323 T4899 T4898 punct ", ",p53PmlEag
R1324 T4900 T4901 det a,plasmid
R1325 T4901 T4898 appos plasmid,p53PmlEag
R1326 T4902 T4901 acl containing,plasmid
R1327 T4903 T4904 nmod exons,11
R1328 T4904 T4902 dobj 11,containing
R1329 T4905 T4904 nummod 2,11
R1330 T4906 T4904 punct –,11
R1331 T4907 T4904 prep of,11
R1332 T4908 T4907 pobj p53,of
R1333 T4909 T4886 punct .,digested
R1334 T4911 T4912 nsubj We,inserted
R1335 T4913 T4912 advmod then,inserted
R1336 T4914 T4915 det a,fragment
R1337 T4915 T4912 dobj fragment,inserted
R1338 T4916 T4917 nummod 5.5,kb
R1339 T4917 T4915 compound kb,fragment
R1340 T4918 T4919 compound ClaI,EagI
R1341 T4919 T4915 compound EagI,fragment
R1342 T4920 T4919 punct -,EagI
R1343 T4921 T4915 prep from,fragment
R1344 T4922 T4921 pobj p53PmlEag,from
R1345 T4923 T4915 prep in,fragment
R1346 T4924 T4925 compound plasmid,F
R1347 T4925 T4923 pobj F,in
R1348 T4926 T4925 compound CN,F
R1349 T4927 T4925 punct -,F
R1350 T4928 T4925 compound PuroΔTK,F
R1351 T4929 T4925 punct -,F
R1352 T4930 T4915 acl digested,fragment
R1353 T4931 T4930 agent by,digested
R1354 T4932 T4931 pobj ClaI,by
R1355 T4933 T4932 cc and,ClaI
R1356 T4934 T4932 conj NotI,ClaI
R1357 T4935 T4912 punct ", ",inserted
R1358 T4936 T4912 cc and,inserted
R1359 T4937 T4912 conj inserted,inserted
R1360 T4938 T4939 det the,fragment
R1361 T4939 T4937 dobj fragment,inserted
R1362 T4940 T4939 amod resulting,fragment
R1363 T4941 T4937 prep between,inserted
R1364 T4942 T4943 det the,sites
R1365 T4943 T4941 pobj sites,between
R1366 T4944 T4943 compound loxP,sites
R1367 T4945 T4943 prep of,sites
R1368 T4946 T4947 compound L3,1L
R1369 T4947 T4945 pobj 1L,of
R1370 T4948 T4947 punct -,1L
R1371 T4949 T4947 compound CF,1L
R1372 T4950 T4947 punct -,1L
R1373 T4951 T4937 prep by,inserted
R1374 T4952 T4953 nmod ClaI,digestion
R1375 T4953 T4951 pobj digestion,by
R1376 T4954 T4952 cc and,ClaI
R1377 T4955 T4952 conj FseI,ClaI
R1378 T4956 T4937 punct ", ",inserted
R1379 T4957 T4937 advcl leading,inserted
R1380 T4958 T4957 prep to,leading
R1381 T4959 T4960 compound L3,1L
R1382 T4960 T4958 pobj 1L,to
R1383 T4961 T4960 punct -,1L
R1384 T4962 T4960 compound p53PmlEagPuroΔTK,1L
R1385 T4963 T4960 punct -,1L
R1386 T4964 T4912 punct .,inserted
R1387 T4966 T4967 nsubj We,engineered
R1388 T4967 T4969 ccomp engineered,performed
R1389 T4968 T4967 advmod next,engineered
R1390 T4970 T4971 det a,plasmid
R1391 T4971 T4967 dobj plasmid,engineered
R1392 T4972 T4971 acl containing,plasmid
R1393 T4973 T4974 det the,region
R1394 T4974 T4972 dobj region,containing
R1395 T4975 T4974 prep for,region
R1396 T4976 T4975 pobj 3,for
R1397 T4977 T4976 punct ′,3
R1398 T4978 T4976 punct -,3
R1399 T4979 T4976 amod homology,3
R1400 T4980 T4972 advmod downstream,containing
R1401 T4981 T4980 prep of,downstream
R1402 T4982 T4983 det the,gene
R1403 T4983 T4981 pobj gene,of
R1404 T4984 T4983 compound p53,gene
R1405 T4985 T4983 cc and,gene
R1406 T4986 T4987 det the,gene
R1407 T4987 T4983 conj gene,gene
R1408 T4988 T4987 compound DTA,gene
R1409 T4989 T4967 prep in,engineered
R1410 T4990 T4991 nummod two,steps
R1411 T4991 T4989 pobj steps,in
R1412 T4992 T4969 punct : ,performed
R1413 T4993 T4994 punct (,i
R1414 T4994 T4969 meta i,performed
R1415 T4995 T4994 punct ),i
R1416 T4996 T4969 nsubj we,performed
R1417 T4997 T4998 det a,ligation
R1418 T4998 T4969 dobj ligation,performed
R1419 T4999 T5000 nummod three,way
R1420 T5000 T4998 compound way,ligation
R1421 T5001 T5000 punct -,way
R1422 T5002 T4998 prep between,ligation
R1423 T5003 T5004 det a,pBS
R1424 T5004 T5002 pobj pBS,between
R1425 T5005 T5004 amod modified,pBS
R1426 T5006 T5004 acl digested,pBS
R1427 T5007 T5006 agent by,digested
R1428 T5008 T5007 pobj HindIII,by
R1429 T5009 T5008 cc and,HindIII
R1430 T5010 T5008 conj NotI,HindIII
R1431 T5011 T5004 punct ", ",pBS
R1432 T5012 T5013 det a,fragment
R1433 T5013 T5004 conj fragment,pBS
R1434 T5014 T5015 compound HindIII,EcoRI
R1435 T5015 T5013 compound EcoRI,fragment
R1436 T5016 T5015 punct -,EcoRI
R1437 T5017 T5013 prep from,fragment
R1438 T5018 T5017 pobj Trp53,from
R1439 T5019 T5013 prep for,fragment
R1440 T5020 T5021 nummod 3,homology
R1441 T5021 T5019 pobj homology,for
R1442 T5022 T5020 punct ′,3
R1443 T5023 T5013 cc and,fragment
R1444 T5024 T5025 det an,fragment
R1445 T5025 T5013 conj fragment,fragment
R1446 T5026 T5027 compound EcoRI,NotI
R1447 T5027 T5025 compound NotI,fragment
R1448 T5028 T5027 punct -,NotI
R1449 T5029 T5025 acl containing,fragment
R1450 T5030 T5031 det the,gene
R1451 T5031 T5029 dobj gene,containing
R1452 T5032 T5031 compound DTA,gene
R1453 T5033 T5025 punct ", ",fragment
R1454 T5034 T5025 prep from,fragment
R1455 T5035 T5036 compound plasmid,pgkdtabpa
R1456 T5036 T5034 pobj pgkdtabpa,from
R1457 T5037 T5025 punct (,fragment
R1458 T5038 T5039 amod kind,gift
R1459 T5039 T5025 appos gift,fragment
R1460 T5040 T5039 prep of,gift
R1461 T5041 T5042 compound P.,Soriano
R1462 T5042 T5040 pobj Soriano,of
R1463 T5043 T4969 punct ),performed
R1464 T5044 T4969 punct ", ",performed
R1465 T5045 T4969 advcl leading,performed
R1466 T5046 T5045 prep to,leading
R1467 T5047 T5048 nmod plasmid,DTA
R1468 T5048 T5046 pobj DTA,to
R1469 T5049 T5048 nummod 3,DTA
R1470 T5050 T5049 punct ′,3
R1471 T5051 T5049 punct +,3
R1472 T5052 T4969 cc and,performed
R1473 T5053 T5054 punct (,ii
R1474 T5054 T5055 meta ii,deleted
R1475 T5055 T4969 conj deleted,performed
R1476 T5056 T5054 punct ),ii
R1477 T5057 T5058 mark because,contains
R1478 T5058 T5055 advcl contains,deleted
R1479 T5059 T5060 det the,region
R1480 T5060 T5058 nsubj region,contains
R1481 T5061 T5062 compound Bsu36I,EcoRI
R1482 T5062 T5060 compound EcoRI,region
R1483 T5063 T5062 punct -,EcoRI
R1484 T5064 T5060 advmod downstream,region
R1485 T5065 T5064 prep of,downstream
R1486 T5066 T5065 pobj p53,of
R1487 T5067 T5068 amod repetitive,sequences
R1488 T5068 T5058 dobj sequences,contains
R1489 T5069 T5070 punct (,F.
R1490 T5070 T5058 meta F.,contains
R1491 T5071 T5070 nmod Toledo,F.
R1492 T5072 T5070 cc and,F.
R1493 T5073 T5070 conj G.,F.
R1494 T5074 T5073 nmod M.,G.
R1495 T5075 T5073 nmod Wahl,G.
R1496 T5076 T5073 punct ", ",G.
R1497 T5077 T5078 amod unpublished,data
R1498 T5078 T5073 conj data,G.
R1499 T5079 T5078 punct ),data
R1500 T5080 T5055 punct ", ",deleted
R1501 T5081 T5055 nsubj we,deleted
R1502 T5082 T5055 advmod later,deleted
R1503 T5083 T5084 det this,region
R1504 T5084 T5055 dobj region,deleted
R1505 T5085 T5055 punct ", ",deleted
R1506 T5086 T5087 aux to,obtain
R1507 T5087 T5055 advcl obtain,deleted
R1508 T5088 T5089 nmod plasmid,DTA
R1509 T5089 T5087 dobj DTA,obtain
R1510 T5090 T5089 nummod 3,DTA
R1511 T5091 T5090 punct ′,3
R1512 T5092 T5090 punct +,3
R1513 T5093 T4969 punct .,performed
R1514 T5095 T5096 det The,homology
R1515 T5096 T5099 nsubj homology,consists
R1516 T5097 T5096 nummod 5,homology
R1517 T5098 T5097 punct ′,5
R1518 T5100 T5099 prep of,consists
R1519 T5101 T5102 det a,fragment
R1520 T5102 T5100 pobj fragment,of
R1521 T5103 T5104 nummod 3.4,kb
R1522 T5104 T5105 npadvmod kb,long
R1523 T5105 T5102 amod long,fragment
R1524 T5106 T5105 punct -,long
R1525 T5107 T5108 compound BamHI,PmlI
R1526 T5108 T5102 compound PmlI,fragment
R1527 T5109 T5108 punct -,PmlI
R1528 T5110 T5102 prep from,fragment
R1529 T5111 T5110 pobj intron,from
R1530 T5112 T5111 nummod 1,intron
R1531 T5113 T5111 prep of,intron
R1532 T5114 T5113 pobj p53,of
R1533 T5115 T5102 acl cloned,fragment
R1534 T5116 T5115 prep in,cloned
R1535 T5117 T5118 det a,pBS
R1536 T5118 T5116 pobj pBS,in
R1537 T5119 T5118 amod modified,pBS
R1538 T5120 T5118 punct (,pBS
R1539 T5121 T5122 compound plasmid,p5
R1540 T5122 T5118 appos p5,pBS
R1541 T5123 T5122 punct ′,p5
R1542 T5124 T5099 punct ),consists
R1543 T5125 T5099 punct .,consists
R1544 T5127 T5128 advmod Finally,assembled
R1545 T5129 T5128 punct ", ",assembled
R1546 T5130 T5131 amod appropriate,fragments
R1547 T5131 T5128 nsubjpass fragments,assembled
R1548 T5132 T5131 prep from,fragments
R1549 T5133 T5134 compound plasmids,p5
R1550 T5134 T5132 pobj p5,from
R1551 T5135 T5134 punct ′,p5
R1552 T5136 T5134 punct ", ",p5
R1553 T5137 T5138 compound L3,1L
R1554 T5138 T5134 conj 1L,p5
R1555 T5139 T5138 punct -,1L
R1556 T5140 T5138 compound p53PmlEagPuroΔTK,1L
R1557 T5141 T5138 punct -,1L
R1558 T5142 T5138 punct ", ",1L
R1559 T5143 T5138 cc and,1L
R1560 T5144 T5145 nummod 3,DTA
R1561 T5145 T5138 conj DTA,1L
R1562 T5146 T5144 punct ′,3
R1563 T5147 T5144 punct +,3
R1564 T5148 T5128 auxpass were,assembled
R1565 T5149 T5128 prep in,assembled
R1566 T5150 T5151 det a,plasmid
R1567 T5151 T5149 pobj plasmid,in
R1568 T5152 T5151 amod modified,plasmid
R1569 T5153 T5151 compound pSP72,plasmid
R1570 T5154 T5155 punct (,Promega
R1571 T5155 T5151 parataxis Promega,plasmid
R1572 T5156 T5155 punct ),Promega
R1573 T5157 T5128 punct .,assembled
R1574 T5159 T5160 compound Plasmid,Flox
R1575 T5160 T5161 nsubjpass Flox,verified
R1576 T5162 T5160 punct ", ",Flox
R1577 T5163 T5164 det the,construct
R1578 T5164 T5160 appos construct,Flox
R1579 T5165 T5164 amod resulting,construct
R1580 T5166 T5164 compound targeting,construct
R1581 T5167 T5161 punct ", ",verified
R1582 T5168 T5161 auxpass was,verified
R1583 T5169 T5161 prep by,verified
R1584 T5170 T5171 compound restriction,analysis
R1585 T5171 T5169 pobj analysis,by
R1586 T5172 T5161 punct ", ",verified
R1587 T5173 T5161 advmod then,verified
R1588 T5174 T5161 dep sequenced,verified
R1589 T5175 T5174 advcl using,sequenced
R1590 T5176 T5177 nummod 30,primers
R1591 T5177 T5175 dobj primers,using
R1592 T5178 T5177 acl chosen,primers
R1593 T5179 T5180 aux to,verify
R1594 T5180 T5178 advcl verify,chosen
R1595 T5181 T5180 advmod precisely,verify
R1596 T5182 T5183 det all,sequences
R1597 T5183 T5180 dobj sequences,verify
R1598 T5184 T5185 compound p53,coding
R1599 T5185 T5183 compound coding,sequences
R1600 T5186 T5183 punct ", ",sequences
R1601 T5187 T5188 det all,junctions
R1602 T5188 T5183 conj junctions,sequences
R1603 T5189 T5190 compound exon,intron
R1604 T5190 T5188 compound intron,junctions
R1605 T5191 T5190 punct –,intron
R1606 T5192 T5188 cc and,junctions
R1607 T5193 T5194 det the,sequences
R1608 T5194 T5188 conj sequences,junctions
R1609 T5195 T5194 prep at,sequences
R1610 T5196 T5195 cc and,at
R1611 T5197 T5195 conj around,at
R1612 T5198 T5199 det the,sites
R1613 T5199 T5197 pobj sites,around
R1614 T5200 T5199 compound loxP,sites
R1615 T5201 T5161 punct .,verified

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id Subject Object Predicate Lexical cue
T4525 10-19 SO_EXT:engineered_biological_sequence denotes construct
T4526 26-29 PR_EXT:000003035 denotes p53
T4527 59-66 SO_EXT:0000155 denotes plasmid
T4528 179-186 SO_EXT:0000155 denotes plasmid
T4529 217-227 SO_EXT:0000346 denotes loxP sites
T4530 239-245 SO_EXT:sequence_altered_entity denotes mutant
T4531 288-296 SO_EXT:sequence_inversion_process denotes inverted
T4532 297-299 SO_EXT:wild_type_entity_or_quality denotes WT
T4533 300-304 SO_EXT:0000346 denotes loxP
T4534 311-313 SO_EXT:wild_type_entity_or_quality denotes WT
T4535 314-318 SO_EXT:0000346 denotes loxP
T4536 354-363 SO_EXT:biological_sequence denotes sequences
T4537 376-384 SO_EXT:biological_sequence denotes sequence
T4538 407-409 SO_EXT:wild_type_entity_or_quality denotes WT
T4539 410-414 SO_EXT:0000346 denotes loxP
T4540 457-466 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T4541 489-497 SO_EXT:biological_sequence denotes sequence
T4542 512-521 GO_SO_EXT:sequence_rearrangement_process denotes recombine
T4543 527-529 SO_EXT:wild_type_entity_or_quality denotes WT
T4544 530-534 SO_EXT:0000346 denotes loxP
T4545 571-575 CL_GO_EXT:cell denotes cell
T4546 608-618 SO_EXT:0000346 denotes loxP sites
T4547 680-685 CL_GO_EXT:cell denotes cells
T4548 739-744 CL_GO_EXT:cell denotes cells
T4549 761-768 SO_EXT:0000155 denotes plasmid
T4550 779-787 SO_EXT:sequence_alteration_process denotes modified
T4551 834-844 SO_EXT:0000346 denotes LoxP sites
T4552 857-864 SO_EXT:0000155 denotes plasmid
T4553 883-891 SO_EXT:sequence_alteration_process denotes modified
T4554 902-909 SO_EXT:0000155 denotes plasmid
T4555 956-972 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotides
T4556 996-1006 SO_EXT:sequence_downstreamness denotes downstream
T4557 1022-1026 SO_EXT:0000704 denotes gene
T4558 1053-1061 SO_EXT:sequence_upstreamness denotes upstream
T4559 1079-1089 SO_EXT:sequence_downstreamness denotes downstream
T4560 1097-1101 SO_EXT:0000704 denotes gene
T4561 1114-1121 SO_EXT:0000155 denotes plasmid
T4562 1160-1161 CHEBI_SO_EXT:base denotes b
T4563 1176-1181 PR_EXT:000003035 denotes Trp53
T4564 1201-1209 SO_EXT:sequence_alteration_process denotes modified
T4565 1265-1272 SO_EXT:0000155 denotes plasmid
T4566 1347-1354 SO_EXT:0000155 denotes plasmid
T4567 1366-1371 SO_EXT:0000147 denotes exons
T4568 1380-1383 PR_EXT:000003035 denotes p53
T4569 1393-1401 SO_EXT:sequence_insertion_process denotes inserted
T4570 1409-1410 CHEBI_SO_EXT:base denotes b
T4571 1448-1455 SO_EXT:0000155 denotes plasmid
T4572 1500-1508 SO_EXT:sequence_insertion_process denotes inserted
T4573 1544-1554 SO_EXT:0000346 denotes loxP sites
T4574 1651-1658 SO_EXT:0000155 denotes plasmid
T4575 1697-1707 SO_EXT:sequence_downstreamness denotes downstream
T4576 1715-1718 PR_EXT:000003035 denotes p53
T4577 1719-1723 SO_EXT:0000704 denotes gene
T4578 1736-1740 SO_EXT:0000704 denotes gene
T4579 1784-1792 GO:0006266 denotes ligation
T4580 1803-1811 SO_EXT:sequence_alteration_process denotes modified
T4581 1828-1835 PR_EXT:P43870 denotes HindIII
T4582 1848-1855 PR_EXT:P43870 denotes HindIII
T4583 1876-1881 PR_EXT:000003035 denotes Trp53
T4584 1943-1947 SO_EXT:0000704 denotes gene
T4585 1954-1961 SO_EXT:0000155 denotes plasmid
T4586 2010-2017 SO_EXT:0000155 denotes plasmid
T4587 2068-2078 SO_EXT:sequence_downstreamness denotes downstream
T4588 2082-2085 PR_EXT:000003035 denotes p53
T4589 2095-2105 SO_EXT:sequence_repeat_unit_or_region denotes repetitive
T4590 2106-2115 SO_EXT:biological_sequence denotes sequences
T4591 2171-2178 SO_EXT:sequence_deletion_process denotes deleted
T4592 2202-2209 SO_EXT:0000155 denotes plasmid
T4593 2255-2256 CHEBI_SO_EXT:base denotes b
T4594 2262-2267 PR_EXT:P23940 denotes BamHI
T4595 2287-2293 SO_EXT:0000188 denotes intron
T4596 2299-2302 PR_EXT:000003035 denotes p53
T4597 2303-2309 SO_EXT:sequence_cloning_process denotes cloned
T4598 2315-2323 SO_EXT:sequence_alteration_process denotes modified
T4599 2329-2336 SO_EXT:0000155 denotes plasmid
T4600 2379-2387 SO_EXT:0000155 denotes plasmids
T4601 2450-2458 SO_EXT:sequence_alteration_process denotes modified
T4602 2465-2472 SO_EXT:0000155 denotes plasmid
T4603 2484-2491 SO_EXT:0000155 denotes Plasmid
T4604 2522-2531 SO_EXT:engineered_biological_sequence denotes construct
T4605 2595-2602 SO_EXT:0000112 denotes primers
T4606 2634-2637 PR_EXT:000003035 denotes p53
T4607 2638-2654 SO_EXT:coding_sequence denotes coding sequences
T4608 2660-2681 SO_EXT:0001421 denotes exon–intron junctions
T4609 2690-2699 SO_EXT:biological_sequence denotes sequences
T4610 2718-2728 SO_EXT:0000346 denotes loxP sites

craft-ca-core-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id Subject Object Predicate Lexical cue
T4478 26-29 PR:000003035 denotes p53
T4479 59-66 SO:0000155 denotes plasmid
T4480 179-186 SO:0000155 denotes plasmid
T4481 217-227 SO:0000346 denotes loxP sites
T4482 300-304 SO:0000346 denotes loxP
T4483 314-318 SO:0000346 denotes loxP
T4484 410-414 SO:0000346 denotes loxP
T4485 530-534 SO:0000346 denotes loxP
T4486 608-618 SO:0000346 denotes loxP sites
T4487 761-768 SO:0000155 denotes plasmid
T4488 834-844 SO:0000346 denotes LoxP sites
T4489 857-864 SO:0000155 denotes plasmid
T4490 902-909 SO:0000155 denotes plasmid
T4491 1022-1026 SO:0000704 denotes gene
T4492 1097-1101 SO:0000704 denotes gene
T4493 1114-1121 SO:0000155 denotes plasmid
T4494 1176-1181 PR:000003035 denotes Trp53
T4495 1265-1272 SO:0000155 denotes plasmid
T4496 1347-1354 SO:0000155 denotes plasmid
T4497 1366-1371 SO:0000147 denotes exons
T4498 1380-1383 PR:000003035 denotes p53
T4499 1448-1455 SO:0000155 denotes plasmid
T4500 1544-1554 SO:0000346 denotes loxP sites
T4501 1651-1658 SO:0000155 denotes plasmid
T4502 1715-1718 PR:000003035 denotes p53
T4503 1719-1723 SO:0000704 denotes gene
T4504 1736-1740 SO:0000704 denotes gene
T4505 1784-1792 GO:0006266 denotes ligation
T4506 1828-1835 PR:P43870 denotes HindIII
T4507 1848-1855 PR:P43870 denotes HindIII
T4508 1876-1881 PR:000003035 denotes Trp53
T4509 1943-1947 SO:0000704 denotes gene
T4510 1954-1961 SO:0000155 denotes plasmid
T4511 2010-2017 SO:0000155 denotes plasmid
T4512 2082-2085 PR:000003035 denotes p53
T4513 2202-2209 SO:0000155 denotes plasmid
T4514 2262-2267 PR:P23940 denotes BamHI
T4515 2287-2293 SO:0000188 denotes intron
T4516 2299-2302 PR:000003035 denotes p53
T4517 2329-2336 SO:0000155 denotes plasmid
T4518 2379-2387 SO:0000155 denotes plasmids
T4519 2465-2472 SO:0000155 denotes plasmid
T4520 2484-2491 SO:0000155 denotes Plasmid
T4521 2595-2602 SO:0000112 denotes primers
T4522 2634-2637 PR:000003035 denotes p53
T4523 2660-2681 SO:0001421 denotes exon–intron junctions
T4524 2718-2728 SO:0000346 denotes loxP sites

2_test

Id Subject Object Predicate Lexical cue
16870721-16204450-76660901 274-276 16204450 denotes 14
16870721-16204450-76660902 746-748 16204450 denotes 14