PMC:1540739 / 29682-30152 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1540739","sourcedb":"PMC","sourceid":"1540739","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1540739","text":"In ES cells, targeting p53ΔPGFP or p53GFP by RMCE was ∼20 times more efficient than targeting Flox by homologous recombination. In MEFs, due to p53GFP toxicity, only the targeting efficiency of RMCE with p53ΔPGFP could be evaluated. Importantly, a phenotypic read-out of the p53ΔPGFP mutation in MEFs, if it had been performed after targeting in fibroblasts by homologous recombination, would have required two rounds of inefficient targeting to target both p53 alleles.","tracks":[]}