PMC:1540739 / 11761-11976
Annnotations
craft-sa-dev
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total of 8 × 105 p53RMCE/+ ES cells were grown without puromycin for 12 h, electroporated with 15 μg CMV-Cre plasmid (pOG231) and 200 μg of the exchange construct, and plated in T25 flasks at 105 cells per flask."}
craft-ca-core-ex-dev
{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T7178","span":{"begin":20,"end":23},"obj":"PR_EXT:000003035"},{"id":"T7179","span":{"begin":28,"end":29},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T7180","span":{"begin":30,"end":38},"obj":"CL:0002322"},{"id":"T7181","span":{"begin":33,"end":38},"obj":"CL_GO_EXT:cell"},{"id":"T7182","span":{"begin":44,"end":49},"obj":"GO:0040007"},{"id":"T7183","span":{"begin":58,"end":67},"obj":"CHEBI:17939"},{"id":"T7184","span":{"begin":104,"end":107},"obj":"NCBITaxon:10358"},{"id":"T7185","span":{"begin":112,"end":119},"obj":"SO_EXT:0000155"},{"id":"T7186","span":{"begin":147,"end":155},"obj":"SO_EXT:sequence_substitution_process"},{"id":"T7187","span":{"begin":156,"end":165},"obj":"SO_EXT:engineered_biological_sequence"},{"id":"T7188","span":{"begin":199,"end":204},"obj":"CL_GO_EXT:cell"}],"text":"\nA total of 8 × 105 p53RMCE/+ ES cells were grown without puromycin for 12 h, electroporated with 15 μg CMV-Cre plasmid (pOG231) and 200 μg of the exchange construct, and plated in T25 flasks at 105 cells per flask."}
craft-ca-core-dev
{"project":"craft-ca-core-dev","denotations":[{"id":"T7169","span":{"begin":20,"end":23},"obj":"PR:000003035"},{"id":"T7170","span":{"begin":30,"end":38},"obj":"CL:0002322"},{"id":"T7171","span":{"begin":44,"end":49},"obj":"GO:0040007"},{"id":"T7172","span":{"begin":58,"end":67},"obj":"CHEBI:17939"},{"id":"T7173","span":{"begin":104,"end":107},"obj":"NCBITaxon:10358"},{"id":"T7174","span":{"begin":112,"end":119},"obj":"SO:0000155"}],"text":"\nA total of 8 × 105 p53RMCE/+ ES cells were grown without puromycin for 12 h, electroporated with 15 μg CMV-Cre plasmid (pOG231) and 200 μg of the exchange construct, and plated in T25 flasks at 105 cells per flask."}