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PMC:1540739 / 11734-12216 JSONTXT

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craft-sa-dev

Id Subject Object Predicate Lexical cue
T7192 0-10 VBG denotes Performing
T7193 11-15 NN denotes RMCE
T7194 16-18 IN denotes in
T7195 19-21 NN denotes ES
T7196 22-27 NNS denotes cells
T7197 27-242 sentence denotes A total of 8 × 105 p53RMCE/+ ES cells were grown without puromycin for 12 h, electroporated with 15 μg CMV-Cre plasmid (pOG231) and 200 μg of the exchange construct, and plated in T25 flasks at 105 cells per flask.
T7198 28-29 DT denotes A
T7199 30-35 NN denotes total
T7200 71-76 VBN denotes grown
T7201 36-38 IN denotes of
T7202 39-40 CD denotes 8
T7203 43-46 CD denotes 105
T7204 41-42 HYPH denotes ×
T7205 60-65 NNS denotes cells
T7206 47-54 NN denotes p53RMCE
T7207 54-55 HYPH denotes /
T7208 55-56 SYM denotes +
T7209 57-59 NN denotes ES
T7210 66-70 VBD denotes were
T7211 77-84 IN denotes without
T7212 85-94 NN denotes puromycin
T7213 95-98 IN denotes for
T7214 99-101 CD denotes 12
T7215 102-103 NN denotes h
T7216 103-105 , denotes ,
T7217 105-119 VBN denotes electroporated
T7218 120-124 IN denotes with
T7219 125-127 CD denotes 15
T7220 139-146 NN denotes plasmid
T7221 128-130 NN denotes μg
T7222 131-134 NN denotes CMV
T7223 135-138 NN denotes Cre
T7224 134-135 HYPH denotes -
T7225 147-148 -LRB- denotes (
T7226 148-154 NN denotes pOG231
T7227 154-155 -RRB- denotes )
T7228 156-159 CC denotes and
T7229 160-163 CD denotes 200
T7230 164-166 NN denotes μg
T7231 167-169 IN denotes of
T7232 170-173 DT denotes the
T7233 183-192 NN denotes construct
T7234 174-182 NN denotes exchange
T7235 192-194 , denotes ,
T7236 194-197 CC denotes and
T7237 198-204 VBN denotes plated
T7238 205-207 IN denotes in
T7239 208-211 NN denotes T25
T7240 212-218 NNS denotes flasks
T7241 219-221 IN denotes at
T7242 222-225 CD denotes 105
T7243 226-231 NNS denotes cells
T7244 232-235 IN denotes per
T7245 236-241 NN denotes flask
T7246 241-242 . denotes .
T7247 242-303 sentence denotes FIAU was added to the medium 3–4 days after electroporation.
T7248 243-247 NN denotes FIAU
T7249 252-257 VBN denotes added
T7250 248-251 VBD denotes was
T7251 258-260 IN denotes to
T7252 261-264 DT denotes the
T7253 265-271 NN denotes medium
T7254 272-273 CD denotes 3
T7255 274-275 CD denotes 4
T7256 273-274 HYPH denotes –
T7257 276-280 NNS denotes days
T7258 281-286 IN denotes after
T7259 287-302 NN denotes electroporation
T7260 302-303 . denotes .
T7261 303-482 sentence denotes Individual clones, picked 10 days after electroporation, were grown in 96-well plates and expanded to generate duplicate plates for freezing and DNA analysis by PCR and Southern.
T7262 304-314 JJ denotes Individual
T7263 315-321 NNS denotes clones
T7264 366-371 VBN denotes grown
T7265 321-323 , denotes ,
T7266 323-329 VBN denotes picked
T7267 330-332 CD denotes 10
T7268 333-337 NNS denotes days
T7269 338-343 IN denotes after
T7270 344-359 NN denotes electroporation
T7271 359-361 , denotes ,
T7272 361-365 VBD denotes were
T7273 372-374 IN denotes in
T7274 375-377 CD denotes 96
T7275 378-382 NN denotes well
T7276 377-378 HYPH denotes -
T7277 383-389 NNS denotes plates
T7278 390-393 CC denotes and
T7279 394-402 VBN denotes expanded
T7280 403-405 TO denotes to
T7281 406-414 VB denotes generate
T7282 415-424 JJ denotes duplicate
T7283 425-431 NNS denotes plates
T7284 432-435 IN denotes for
T7285 436-444 VBG denotes freezing
T7286 445-448 CC denotes and
T7287 449-452 NN denotes DNA
T7288 453-461 NN denotes analysis
T7289 462-464 IN denotes by
T7290 465-468 NN denotes PCR
T7291 469-472 CC denotes and
T7292 473-481 NNP denotes Southern
T7293 481-482 . denotes .
R2183 T7193 T7192 dobj RMCE,Performing
R2184 T7194 T7192 prep in,Performing
R2185 T7195 T7196 compound ES,cells
R2186 T7196 T7194 pobj cells,in
R2187 T7198 T7199 det A,total
R2188 T7199 T7200 nsubjpass total,grown
R2189 T7201 T7199 prep of,total
R2190 T7202 T7203 compound 8,105
R2191 T7203 T7205 nummod 105,cells
R2192 T7204 T7203 punct ×,105
R2193 T7205 T7201 pobj cells,of
R2194 T7206 T7205 nmod p53RMCE,cells
R2195 T7207 T7206 punct /,p53RMCE
R2196 T7208 T7206 punct +,p53RMCE
R2197 T7209 T7205 compound ES,cells
R2198 T7210 T7200 auxpass were,grown
R2199 T7211 T7200 prep without,grown
R2200 T7212 T7211 pobj puromycin,without
R2201 T7213 T7200 prep for,grown
R2202 T7214 T7215 nummod 12,h
R2203 T7215 T7213 pobj h,for
R2204 T7216 T7200 punct ", ",grown
R2205 T7217 T7200 conj electroporated,grown
R2206 T7218 T7217 prep with,electroporated
R2207 T7219 T7220 nummod 15,plasmid
R2208 T7220 T7218 pobj plasmid,with
R2209 T7221 T7220 compound μg,plasmid
R2210 T7222 T7223 compound CMV,Cre
R2211 T7223 T7220 compound Cre,plasmid
R2212 T7224 T7223 punct -,Cre
R2213 T7225 T7226 punct (,pOG231
R2214 T7226 T7220 parataxis pOG231,plasmid
R2215 T7227 T7226 punct ),pOG231
R2216 T7228 T7220 cc and,plasmid
R2217 T7229 T7230 nummod 200,μg
R2218 T7230 T7220 conj μg,plasmid
R2219 T7231 T7230 prep of,μg
R2220 T7232 T7233 det the,construct
R2221 T7233 T7231 pobj construct,of
R2222 T7234 T7233 compound exchange,construct
R2223 T7235 T7217 punct ", ",electroporated
R2224 T7236 T7217 cc and,electroporated
R2225 T7237 T7217 conj plated,electroporated
R2226 T7238 T7237 prep in,plated
R2227 T7239 T7240 compound T25,flasks
R2228 T7240 T7238 pobj flasks,in
R2229 T7241 T7237 prep at,plated
R2230 T7242 T7243 nummod 105,cells
R2231 T7243 T7241 pobj cells,at
R2232 T7244 T7243 prep per,cells
R2233 T7245 T7244 pobj flask,per
R2234 T7246 T7200 punct .,grown
R2235 T7248 T7249 nsubjpass FIAU,added
R2236 T7250 T7249 auxpass was,added
R2237 T7251 T7249 prep to,added
R2238 T7252 T7253 det the,medium
R2239 T7253 T7251 pobj medium,to
R2240 T7254 T7255 compound 3,4
R2241 T7255 T7257 nummod 4,days
R2242 T7256 T7255 punct –,4
R2243 T7257 T7258 npadvmod days,after
R2244 T7258 T7249 prep after,added
R2245 T7259 T7258 pobj electroporation,after
R2246 T7260 T7249 punct .,added
R2247 T7262 T7263 amod Individual,clones
R2248 T7263 T7264 nsubjpass clones,grown
R2249 T7265 T7263 punct ", ",clones
R2250 T7266 T7263 acl picked,clones
R2251 T7267 T7268 nummod 10,days
R2252 T7268 T7266 npadvmod days,picked
R2253 T7269 T7268 prep after,days
R2254 T7270 T7269 pobj electroporation,after
R2255 T7271 T7264 punct ", ",grown
R2256 T7272 T7264 auxpass were,grown
R2257 T7273 T7264 prep in,grown
R2258 T7274 T7275 nummod 96,well
R2259 T7275 T7277 compound well,plates
R2260 T7276 T7275 punct -,well
R2261 T7277 T7273 pobj plates,in
R2262 T7278 T7264 cc and,grown
R2263 T7279 T7264 conj expanded,grown
R2264 T7280 T7281 aux to,generate
R2265 T7281 T7279 advcl generate,expanded
R2266 T7282 T7283 amod duplicate,plates
R2267 T7283 T7281 dobj plates,generate
R2268 T7284 T7283 prep for,plates
R2269 T7285 T7284 pobj freezing,for
R2270 T7286 T7285 cc and,freezing
R2271 T7287 T7288 compound DNA,analysis
R2272 T7288 T7285 conj analysis,freezing
R2273 T7289 T7281 prep by,generate
R2274 T7290 T7289 pobj PCR,by
R2275 T7291 T7290 cc and,PCR
R2276 T7292 T7290 conj Southern,PCR
R2277 T7293 T7264 punct .,grown

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T7176 19-27 CL:0002322 denotes ES cells
T7177 22-27 CL_GO_EXT:cell denotes cells
T7178 47-50 PR_EXT:000003035 denotes p53
T7179 55-56 SO_EXT:normal_or_wild_type_or_present denotes +
T7180 57-65 CL:0002322 denotes ES cells
T7181 60-65 CL_GO_EXT:cell denotes cells
T7182 71-76 GO:0040007 denotes grown
T7183 85-94 CHEBI:17939 denotes puromycin
T7184 131-134 NCBITaxon:10358 denotes CMV
T7185 139-146 SO_EXT:0000155 denotes plasmid
T7186 174-182 SO_EXT:sequence_substitution_process denotes exchange
T7187 183-192 SO_EXT:engineered_biological_sequence denotes construct
T7188 226-231 CL_GO_EXT:cell denotes cells
T7189 315-321 SO_EXT:sequence_cloned_entity denotes clones
T7190 366-371 GO:0040007 denotes grown
T7191 449-452 CHEBI_SO_EXT:DNA denotes DNA

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T7168 19-27 CL:0002322 denotes ES cells
T7169 47-50 PR:000003035 denotes p53
T7170 57-65 CL:0002322 denotes ES cells
T7171 71-76 GO:0040007 denotes grown
T7172 85-94 CHEBI:17939 denotes puromycin
T7173 131-134 NCBITaxon:10358 denotes CMV
T7174 139-146 SO:0000155 denotes plasmid
T7175 366-371 GO:0040007 denotes grown