PMC:1540739 / 11066-11452 JSONTXT

{"project":"craft-sa-dev","denotations":[{"id":"T6731","span":{"begin":0,"end":7},"obj":"JJ"},{"id":"T6732","span":{"begin":8,"end":12},"obj":"NNS"},{"id":"T6733","span":{"begin":51,"end":59},"obj":"VBN"},{"id":"T6734","span":{"begin":12,"end":14},"obj":","},{"id":"T6735","span":{"begin":14,"end":22},"obj":"VBN"},{"id":"T6736","span":{"begin":23,"end":27},"obj":"IN"},{"id":"T6737","span":{"begin":28,"end":32},"obj":"CD"},{"id":"T6738","span":{"begin":33,"end":36},"obj":"NN"},{"id":"T6739","span":{"begin":37,"end":44},"obj":"NNS"},{"id":"T6740","span":{"begin":44,"end":46},"obj":","},{"id":"T6741","span":{"begin":46,"end":50},"obj":"VBD"},{"id":"T6742","span":{"begin":60,"end":62},"obj":"IN"},{"id":"T6743","span":{"begin":63,"end":67},"obj":"NN"},{"id":"T6744","span":{"begin":68,"end":72},"obj":"IN"},{"id":"T6745","span":{"begin":73,"end":75},"obj":"CD"},{"id":"T6746","span":{"begin":75,"end":76},"obj":"NN"},{"id":"T6747","span":{"begin":77,"end":80},"obj":"NN"},{"id":"T6748","span":{"begin":80,"end":82},"obj":","},{"id":"T6749","span":{"begin":82,"end":85},"obj":"CD"},{"id":"T6750","span":{"begin":86,"end":88},"obj":"NN"},{"id":"T6751","span":{"begin":89,"end":92},"obj":"NN"},{"id":"T6752","span":{"begin":92,"end":94},"obj":","},{"id":"T6753","span":{"begin":94,"end":95},"obj":"CD"},{"id":"T6754","span":{"begin":96,"end":98},"obj":"NN"},{"id":"T6755","span":{"begin":101,"end":110},"obj":"NN"},{"id":"T6756","span":{"begin":99,"end":100},"obj":"NN"},{"id":"T6757","span":{"begin":100,"end":101},"obj":"HYPH"},{"id":"T6758","span":{"begin":111,"end":114},"obj":"CC"},{"id":"T6759","span":{"begin":115,"end":126},"obj":"NNS"},{"id":"T6760","span":{"begin":126,"end":127},"obj":"."},{"id":"T6761","span":{"begin":127,"end":303},"obj":"sentence"},{"id":"T6762","span":{"begin":128,"end":131},"obj":"CD"},{"id":"T6763","span":{"begin":132,"end":137},"obj":"NN"},{"id":"T6764","span":{"begin":131,"end":132},"obj":"HYPH"},{"id":"T6765","span":{"begin":141,"end":146},"obj":"NNS"},{"id":"T6766","span":{"begin":138,"end":140},"obj":"NN"},{"id":"T6767","span":{"begin":152,"end":157},"obj":"VBN"},{"id":"T6768","span":{"begin":147,"end":151},"obj":"VBD"},{"id":"T6769","span":{"begin":158,"end":160},"obj":"IN"},{"id":"T6770","span":{"begin":161,"end":164},"obj":"DT"},{"id":"T6771","span":{"begin":170,"end":176},"obj":"NN"},{"id":"T6772","span":{"begin":165,"end":169},"obj":"JJ"},{"id":"T6773","span":{"begin":177,"end":189},"obj":"VBN"},{"id":"T6774","span":{"begin":190,"end":194},"obj":"IN"},{"id":"T6775","span":{"begin":195,"end":199},"obj":"CD"},{"id":"T6776","span":{"begin":200,"end":201},"obj":"NN"},{"id":"T6777","span":{"begin":205,"end":210},"obj":"NN"},{"id":"T6778","span":{"begin":201,"end":202},"obj":"SYM"},{"id":"T6779","span":{"begin":202,"end":204},"obj":"NN"},{"id":"T6780","span":{"begin":211,"end":212},"obj":"-LRB-"},{"id":"T6781","span":{"begin":212,"end":220},"obj":"NNP"},{"id":"T6782","span":{"begin":220,"end":221},"obj":"-RRB-"},{"id":"T6783","span":{"begin":221,"end":223},"obj":","},{"id":"T6784","span":{"begin":223,"end":225},"obj":"IN"},{"id":"T6785","span":{"begin":226,"end":227},"obj":"DT"},{"id":"T6786","span":{"begin":228,"end":233},"obj":"NN"},{"id":"T6787","span":{"begin":234,"end":236},"obj":"IN"},{"id":"T6788","span":{"begin":237,"end":246},"obj":"NN"},{"id":"T6789","span":{"begin":247,"end":248},"obj":"NN"},{"id":"T6790","span":{"begin":249,"end":256},"obj":"JJ"},{"id":"T6791","span":{"begin":248,"end":249},"obj":"HYPH"},{"id":"T6792","span":{"begin":269,"end":276},"obj":"NNS"},{"id":"T6793","span":{"begin":257,"end":264},"obj":"NN"},{"id":"T6794","span":{"begin":264,"end":265},"obj":"HYPH"},{"id":"T6795","span":{"begin":265,"end":266},"obj":"CD"},{"id":"T6796","span":{"begin":266,"end":267},"obj":"HYPH"},{"id":"T6797","span":{"begin":267,"end":268},"obj":"CD"},{"id":"T6798","span":{"begin":277,"end":278},"obj":"-LRB-"},{"id":"T6799","span":{"begin":283,"end":287},"obj":"NN"},{"id":"T6800","span":{"begin":278,"end":282},"obj":"JJ"},{"id":"T6801","span":{"begin":288,"end":290},"obj":"IN"},{"id":"T6802","span":{"begin":291,"end":293},"obj":"NNP"},{"id":"T6803","span":{"begin":294,"end":301},"obj":"NNP"},{"id":"T6804","span":{"begin":301,"end":302},"obj":"-RRB-"},{"id":"T6805","span":{"begin":302,"end":303},"obj":"."},{"id":"T6806","span":{"begin":303,"end":386},"obj":"sentence"},{"id":"T6807","span":{"begin":304,"end":314},"obj":"NNS"},{"id":"T6808","span":{"begin":320,"end":329},"obj":"VBN"},{"id":"T6809","span":{"begin":315,"end":319},"obj":"VBD"},{"id":"T6810","span":{"begin":330,"end":334},"obj":"IN"},{"id":"T6811","span":{"begin":335,"end":336},"obj":"CD"},{"id":"T6812","span":{"begin":337,"end":339},"obj":"NN"},{"id":"T6813","span":{"begin":343,"end":352},"obj":"NN"},{"id":"T6814","span":{"begin":339,"end":340},"obj":"SYM"},{"id":"T6815","span":{"begin":340,"end":342},"obj":"NN"},{"id":"T6816","span":{"begin":352,"end":354},"obj":","},{"id":"T6817","span":{"begin":354,"end":357},"obj":"CD"},{"id":"T6818","span":{"begin":358,"end":360},"obj":"NN"},{"id":"T6819","span":{"begin":361,"end":365},"obj":"NN"},{"id":"T6820","span":{"begin":366,"end":368},"obj":"CC"},{"id":"T6821","span":{"begin":369,"end":370},"obj":"CD"},{"id":"T6822","span":{"begin":371,"end":373},"obj":"NN"},{"id":"T6823","span":{"begin":374,"end":385},"obj":"NN"},{"id":"T6824","span":{"begin":385,"end":386},"obj":"."}],"relations":[{"id":"R2048","pred":"amod","subj":"T6731","obj":"T6732"},{"id":"R2049","pred":"nsubjpass","subj":"T6732","obj":"T6733"},{"id":"R2050","pred":"punct","subj":"T6734","obj":"T6732"},{"id":"R2051","pred":"acl","subj":"T6735","obj":"T6732"},{"id":"R2052","pred":"prep","subj":"T6736","obj":"T6735"},{"id":"R2053","pred":"nummod","subj":"T6737","obj":"T6738"},{"id":"R2054","pred":"compound","subj":"T6738","obj":"T6739"},{"id":"R2055","pred":"pobj","subj":"T6739","obj":"T6736"},{"id":"R2056","pred":"punct","subj":"T6740","obj":"T6733"},{"id":"R2057","pred":"auxpass","subj":"T6741","obj":"T6733"},{"id":"R2058","pred":"prep","subj":"T6742","obj":"T6733"},{"id":"R2059","pred":"pobj","subj":"T6743","obj":"T6742"},{"id":"R2060","pred":"prep","subj":"T6744","obj":"T6733"},{"id":"R2061","pred":"nummod","subj":"T6745","obj":"T6746"},{"id":"R2062","pred":"compound","subj":"T6746","obj":"T6747"},{"id":"R2063","pred":"pobj","subj":"T6747","obj":"T6744"},{"id":"R2064","pred":"punct","subj":"T6748","obj":"T6747"},{"id":"R2065","pred":"nummod","subj":"T6749","obj":"T6750"},{"id":"R2066","pred":"compound","subj":"T6750","obj":"T6751"},{"id":"R2067","pred":"conj","subj":"T6751","obj":"T6747"},{"id":"R2068","pred":"punct","subj":"T6752","obj":"T6751"},{"id":"R2069","pred":"nummod","subj":"T6753","obj":"T6754"},{"id":"R2070","pred":"compound","subj":"T6754","obj":"T6755"},{"id":"R2071","pred":"conj","subj":"T6755","obj":"T6751"},{"id":"R2072","pred":"compound","subj":"T6756","obj":"T6755"},{"id":"R2073","pred":"punct","subj":"T6757","obj":"T6755"},{"id":"R2074","pred":"cc","subj":"T6758","obj":"T6755"},{"id":"R2075","pred":"conj","subj":"T6759","obj":"T6755"},{"id":"R2076","pred":"punct","subj":"T6760","obj":"T6733"},{"id":"R2077","pred":"nummod","subj":"T6762","obj":"T6763"},{"id":"R2078","pred":"compound","subj":"T6763","obj":"T6765"},{"id":"R2079","pred":"punct","subj":"T6764","obj":"T6763"},{"id":"R2080","pred":"nsubjpass","subj":"T6765","obj":"T6767"},{"id":"R2081","pred":"compound","subj":"T6766","obj":"T6765"},{"id":"R2082","pred":"auxpass","subj":"T6768","obj":"T6767"},{"id":"R2083","pred":"prep","subj":"T6769","obj":"T6767"},{"id":"R2084","pred":"det","subj":"T6770","obj":"T6771"},{"id":"R2085","pred":"pobj","subj":"T6771","obj":"T6769"},{"id":"R2086","pred":"amod","subj":"T6772","obj":"T6771"},{"id":"R2087","pred":"acl","subj":"T6773","obj":"T6771"},{"id":"R2088","pred":"prep","subj":"T6774","obj":"T6773"},{"id":"R2089","pred":"nummod","subj":"T6775","obj":"T6776"},{"id":"R2090","pred":"nmod","subj":"T6776","obj":"T6777"},{"id":"R2091","pred":"pobj","subj":"T6777","obj":"T6774"},{"id":"R2092","pred":"punct","subj":"T6778","obj":"T6779"},{"id":"R2093","pred":"prep","subj":"T6779","obj":"T6776"},{"id":"R2094","pred":"punct","subj":"T6780","obj":"T6781"},{"id":"R2095","pred":"parataxis","subj":"T6781","obj":"T6777"},{"id":"R2096","pred":"punct","subj":"T6782","obj":"T6781"},{"id":"R2097","pred":"punct","subj":"T6783","obj":"T6767"},{"id":"R2098","pred":"prep","subj":"T6784","obj":"T6767"},{"id":"R2099","pred":"det","subj":"T6785","obj":"T6786"},{"id":"R2100","pred":"pobj","subj":"T6786","obj":"T6784"},{"id":"R2101","pred":"prep","subj":"T6787","obj":"T6786"},{"id":"R2102","pred":"compound","subj":"T6788","obj":"T6789"},{"id":"R2103","pred":"npadvmod","subj":"T6789","obj":"T6790"},{"id":"R2104","pred":"amod","subj":"T6790","obj":"T6792"},{"id":"R2105","pred":"punct","subj":"T6791","obj":"T6790"},{"id":"R2106","pred":"pobj","subj":"T6792","obj":"T6787"},{"id":"R2107","pred":"nmod","subj":"T6793","obj":"T6792"},{"id":"R2108","pred":"punct","subj":"T6794","obj":"T6793"},{"id":"R2109","pred":"nummod","subj":"T6795","obj":"T6793"},{"id":"R2110","pred":"punct","subj":"T6796","obj":"T6793"},{"id":"R2111","pred":"nummod","subj":"T6797","obj":"T6793"},{"id":"R2112","pred":"punct","subj":"T6798","obj":"T6799"},{"id":"R2113","pred":"parataxis","subj":"T6799","obj":"T6786"},{"id":"R2114","pred":"amod","subj":"T6800","obj":"T6799"},{"id":"R2115","pred":"prep","subj":"T6801","obj":"T6799"},{"id":"R2116","pred":"compound","subj":"T6802","obj":"T6803"},{"id":"R2117","pred":"pobj","subj":"T6803","obj":"T6801"},{"id":"R2118","pred":"punct","subj":"T6804","obj":"T6799"},{"id":"R2119","pred":"punct","subj":"T6805","obj":"T6767"},{"id":"R2120","pred":"nsubjpass","subj":"T6807","obj":"T6808"},{"id":"R2121","pred":"auxpass","subj":"T6809","obj":"T6808"},{"id":"R2122","pred":"prep","subj":"T6810","obj":"T6808"},{"id":"R2123","pred":"nummod","subj":"T6811","obj":"T6812"},{"id":"R2124","pred":"nmod","subj":"T6812","obj":"T6813"},{"id":"R2125","pred":"pobj","subj":"T6813","obj":"T6810"},{"id":"R2126","pred":"punct","subj":"T6814","obj":"T6815"},{"id":"R2127","pred":"prep","subj":"T6815","obj":"T6812"},{"id":"R2128","pred":"punct","subj":"T6816","obj":"T6813"},{"id":"R2129","pred":"nummod","subj":"T6817","obj":"T6818"},{"id":"R2130","pred":"compound","subj":"T6818","obj":"T6819"},{"id":"R2131","pred":"conj","subj":"T6819","obj":"T6813"},{"id":"R2132","pred":"cc","subj":"T6820","obj":"T6819"},{"id":"R2133","pred":"nummod","subj":"T6821","obj":"T6822"},{"id":"R2134","pred":"compound","subj":"T6822","obj":"T6823"},{"id":"R2135","pred":"conj","subj":"T6823","obj":"T6819"},{"id":"R2136","pred":"punct","subj":"T6824","obj":"T6808"}],"text":"Primary MEFs, isolated from 13.5 day embryos, were cultured in DMEM with 15% FBS, 100 mM BME, 2 mM l-glutamine and antibiotics. 129/SvJae ES cells were grown in the same medium supplemented with 1000 U/ml ESGRO (Chemicon), on a layer of mitomycin C-treated SNLPuro-7/4 feeders (kind gift of A. Bradley). Selections were performed with 2 μg/ml puromycin, 0.2 μM FIAU or 2 μM ganciclovir."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T6717","span":{"begin":37,"end":44},"obj":"UBERON:0000922"},{"id":"T6718","span":{"begin":99,"end":110},"obj":"CHEBI_EXT:L_glutamine"},{"id":"T6719","span":{"begin":101,"end":110},"obj":"CHEBI_SO_EXT:glutamine"},{"id":"T6720","span":{"begin":115,"end":126},"obj":"CHEBI_EXT:33282"},{"id":"T6721","span":{"begin":138,"end":146},"obj":"CL:0002322"},{"id":"T6722","span":{"begin":141,"end":146},"obj":"CL_GO_EXT:cell"},{"id":"T6723","span":{"begin":152,"end":157},"obj":"GO:0040007"},{"id":"T6724","span":{"begin":237,"end":248},"obj":"CHEBI:27504"},{"id":"T6725","span":{"begin":343,"end":352},"obj":"CHEBI:17939"},{"id":"T6726","span":{"begin":374,"end":385},"obj":"CHEBI:465284"}],"text":"Primary MEFs, isolated from 13.5 day embryos, were cultured in DMEM with 15% FBS, 100 mM BME, 2 mM l-glutamine and antibiotics. 129/SvJae ES cells were grown in the same medium supplemented with 1000 U/ml ESGRO (Chemicon), on a layer of mitomycin C-treated SNLPuro-7/4 feeders (kind gift of A. Bradley). Selections were performed with 2 μg/ml puromycin, 0.2 μM FIAU or 2 μM ganciclovir."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T6709","span":{"begin":37,"end":44},"obj":"UBERON:0000922"},{"id":"T6710","span":{"begin":115,"end":126},"obj":"CHEBI:33282"},{"id":"T6711","span":{"begin":138,"end":146},"obj":"CL:0002322"},{"id":"T6712","span":{"begin":152,"end":157},"obj":"GO:0040007"},{"id":"T6713","span":{"begin":237,"end":248},"obj":"CHEBI:27504"},{"id":"T6714","span":{"begin":343,"end":352},"obj":"CHEBI:17939"},{"id":"T6715","span":{"begin":374,"end":385},"obj":"CHEBI:465284"}],"text":"Primary MEFs, isolated from 13.5 day embryos, were cultured in DMEM with 15% FBS, 100 mM BME, 2 mM l-glutamine and antibiotics. 129/SvJae ES cells were grown in the same medium supplemented with 1000 U/ml ESGRO (Chemicon), on a layer of mitomycin C-treated SNLPuro-7/4 feeders (kind gift of A. Bradley). Selections were performed with 2 μg/ml puromycin, 0.2 μM FIAU or 2 μM ganciclovir."}