| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T190 |
7-11 |
NNS |
denotes |
mice |
| T191 |
0-6 |
JJ |
denotes |
mutant |
| T192 |
12-16 |
VBP |
denotes |
show |
| T193 |
17-21 |
JJ |
denotes |
long |
| T194 |
22-26 |
NN |
denotes |
term |
| T195 |
21-22 |
HYPH |
denotes |
- |
| T196 |
39-48 |
NN |
denotes |
retention |
| T197 |
26-28 |
, |
denotes |
, |
| T198 |
28-38 |
JJ |
denotes |
incomplete |
| T199 |
49-51 |
IN |
denotes |
of |
| T200 |
52-56 |
DT |
denotes |
some |
| T201 |
76-87 |
NN |
denotes |
innervation |
| T202 |
57-65 |
JJ |
denotes |
afferent |
| T203 |
66-71 |
JJ |
denotes |
inner |
| T204 |
72-75 |
NN |
denotes |
ear |
| T205 |
87-88 |
sentence |
denotes |
|
| T206 |
98-108 |
sentence |
denotes |
Background |
| T207 |
98-108 |
NN |
denotes |
Background |
| T208 |
108-247 |
sentence |
denotes |
Ears of Brn3c null mutants develop immature hair cells, identifiable only by certain molecular markers, and undergo apoptosis in neonates. |
| T209 |
109-113 |
NNS |
denotes |
Ears |
| T210 |
136-143 |
VBP |
denotes |
develop |
| T211 |
114-116 |
IN |
denotes |
of |
| T212 |
117-122 |
NN |
denotes |
Brn3c |
| T213 |
128-135 |
NNS |
denotes |
mutants |
| T214 |
123-127 |
JJ |
denotes |
null |
| T215 |
144-152 |
JJ |
denotes |
immature |
| T216 |
158-163 |
NNS |
denotes |
cells |
| T217 |
153-157 |
NN |
denotes |
hair |
| T218 |
163-165 |
, |
denotes |
, |
| T219 |
165-177 |
JJ |
denotes |
identifiable |
| T220 |
178-182 |
RB |
denotes |
only |
| T221 |
183-185 |
IN |
denotes |
by |
| T222 |
186-193 |
JJ |
denotes |
certain |
| T223 |
204-211 |
NNS |
denotes |
markers |
| T224 |
194-203 |
JJ |
denotes |
molecular |
| T225 |
211-213 |
, |
denotes |
, |
| T226 |
213-216 |
CC |
denotes |
and |
| T227 |
217-224 |
VBP |
denotes |
undergo |
| T228 |
225-234 |
NN |
denotes |
apoptosis |
| T229 |
235-237 |
IN |
denotes |
in |
| T230 |
238-246 |
NNS |
denotes |
neonates |
| T231 |
246-247 |
. |
denotes |
. |
| T232 |
247-389 |
sentence |
denotes |
This partial development of hair cells could lead to enough neurotrophin expression to sustain sensory neurons through embryonic development. |
| T233 |
248-252 |
DT |
denotes |
This |
| T234 |
261-272 |
NN |
denotes |
development |
| T235 |
253-260 |
JJ |
denotes |
partial |
| T236 |
293-297 |
VB |
denotes |
lead |
| T237 |
273-275 |
IN |
denotes |
of |
| T238 |
276-280 |
NN |
denotes |
hair |
| T239 |
281-286 |
NNS |
denotes |
cells |
| T240 |
287-292 |
MD |
denotes |
could |
| T241 |
298-300 |
IN |
denotes |
to |
| T242 |
301-307 |
JJ |
denotes |
enough |
| T243 |
321-331 |
NN |
denotes |
expression |
| T244 |
308-320 |
NN |
denotes |
neurotrophin |
| T245 |
332-334 |
TO |
denotes |
to |
| T246 |
335-342 |
VB |
denotes |
sustain |
| T247 |
343-350 |
JJ |
denotes |
sensory |
| T248 |
351-358 |
NNS |
denotes |
neurons |
| T249 |
359-366 |
IN |
denotes |
through |
| T250 |
367-376 |
JJ |
denotes |
embryonic |
| T251 |
377-388 |
NN |
denotes |
development |
| T252 |
388-389 |
. |
denotes |
. |
| T253 |
389-507 |
sentence |
denotes |
We have therefore investigated in these mutants the patterns of innervation and of expression of known neurotrophins. |
| T254 |
390-392 |
PRP |
denotes |
We |
| T255 |
408-420 |
VBN |
denotes |
investigated |
| T256 |
393-397 |
VBP |
denotes |
have |
| T257 |
398-407 |
RB |
denotes |
therefore |
| T258 |
421-423 |
IN |
denotes |
in |
| T259 |
424-429 |
DT |
denotes |
these |
| T260 |
430-437 |
NNS |
denotes |
mutants |
| T261 |
438-441 |
DT |
denotes |
the |
| T262 |
442-450 |
NNS |
denotes |
patterns |
| T263 |
451-453 |
IN |
denotes |
of |
| T264 |
454-465 |
NN |
denotes |
innervation |
| T265 |
466-469 |
CC |
denotes |
and |
| T266 |
470-472 |
IN |
denotes |
of |
| T267 |
473-483 |
NN |
denotes |
expression |
| T268 |
484-486 |
IN |
denotes |
of |
| T269 |
487-492 |
JJ |
denotes |
known |
| T270 |
493-506 |
NNS |
denotes |
neurotrophins |
| T271 |
506-507 |
. |
denotes |
. |
| T272 |
507-516 |
sentence |
denotes |
Results |
| T273 |
509-516 |
NNS |
denotes |
Results |
| T274 |
516-718 |
sentence |
denotes |
At birth there is a limited expression of BDNF and NT-3 in the mutant sensory epithelia and DiI tracing shows no specific reduction of afferents or efferents that resembles neurotrophin null mutations. |
| T275 |
517-519 |
IN |
denotes |
At |
| T276 |
532-534 |
VBZ |
denotes |
is |
| T277 |
520-525 |
NN |
denotes |
birth |
| T278 |
526-531 |
EX |
denotes |
there |
| T279 |
535-536 |
DT |
denotes |
a |
| T280 |
545-555 |
NN |
denotes |
expression |
| T281 |
537-544 |
VBN |
denotes |
limited |
| T282 |
556-558 |
IN |
denotes |
of |
| T283 |
559-563 |
NN |
denotes |
BDNF |
| T284 |
564-567 |
CC |
denotes |
and |
| T285 |
568-570 |
NN |
denotes |
NT |
| T286 |
570-571 |
HYPH |
denotes |
- |
| T287 |
571-572 |
CD |
denotes |
3 |
| T288 |
573-575 |
IN |
denotes |
in |
| T289 |
576-579 |
DT |
denotes |
the |
| T290 |
595-604 |
NNS |
denotes |
epithelia |
| T291 |
580-586 |
JJ |
denotes |
mutant |
| T292 |
587-594 |
JJ |
denotes |
sensory |
| T293 |
605-608 |
CC |
denotes |
and |
| T294 |
609-612 |
NN |
denotes |
DiI |
| T295 |
613-620 |
NN |
denotes |
tracing |
| T296 |
621-626 |
VBZ |
denotes |
shows |
| T297 |
627-629 |
DT |
denotes |
no |
| T298 |
639-648 |
NN |
denotes |
reduction |
| T299 |
630-638 |
JJ |
denotes |
specific |
| T300 |
649-651 |
IN |
denotes |
of |
| T301 |
652-661 |
NNS |
denotes |
afferents |
| T302 |
662-664 |
CC |
denotes |
or |
| T303 |
665-674 |
NNS |
denotes |
efferents |
| T304 |
675-679 |
WDT |
denotes |
that |
| T305 |
680-689 |
VBZ |
denotes |
resembles |
| T306 |
690-702 |
NN |
denotes |
neurotrophin |
| T307 |
708-717 |
NNS |
denotes |
mutations |
| T308 |
703-707 |
JJ |
denotes |
null |
| T309 |
717-718 |
. |
denotes |
. |
| T310 |
718-818 |
sentence |
denotes |
At postnatal day 7/8 (P7/8), innervation is severely reduced both qualitatively and quantitatively. |
| T311 |
719-721 |
IN |
denotes |
At |
| T312 |
772-779 |
VBN |
denotes |
reduced |
| T313 |
722-731 |
JJ |
denotes |
postnatal |
| T314 |
732-735 |
NN |
denotes |
day |
| T315 |
736-737 |
CD |
denotes |
7 |
| T316 |
737-738 |
HYPH |
denotes |
/ |
| T317 |
738-739 |
CD |
denotes |
8 |
| T318 |
740-741 |
-LRB- |
denotes |
( |
| T319 |
741-743 |
NN |
denotes |
P7 |
| T320 |
743-744 |
HYPH |
denotes |
/ |
| T321 |
744-745 |
CD |
denotes |
8 |
| T322 |
745-746 |
-RRB- |
denotes |
) |
| T323 |
746-748 |
, |
denotes |
, |
| T324 |
748-759 |
NN |
denotes |
innervation |
| T325 |
760-762 |
VBZ |
denotes |
is |
| T326 |
763-771 |
RB |
denotes |
severely |
| T327 |
780-784 |
CC |
denotes |
both |
| T328 |
785-798 |
RB |
denotes |
qualitatively |
| T329 |
799-802 |
CC |
denotes |
and |
| T330 |
803-817 |
RB |
denotes |
quantitatively |
| T331 |
817-818 |
. |
denotes |
. |
| T332 |
818-926 |
sentence |
denotes |
1% of myosin VIIa-positive immature hair cells are present in the mutant cochlea, concentrated in the base. |
| T333 |
819-820 |
CD |
denotes |
1 |
| T334 |
820-821 |
NN |
denotes |
% |
| T335 |
866-869 |
VBP |
denotes |
are |
| T336 |
822-824 |
IN |
denotes |
of |
| T337 |
825-831 |
NN |
denotes |
myosin |
| T338 |
832-836 |
NN |
denotes |
VIIa |
| T339 |
837-845 |
JJ |
denotes |
positive |
| T340 |
836-837 |
HYPH |
denotes |
- |
| T341 |
860-865 |
NNS |
denotes |
cells |
| T342 |
846-854 |
JJ |
denotes |
immature |
| T343 |
855-859 |
NN |
denotes |
hair |
| T344 |
870-877 |
JJ |
denotes |
present |
| T345 |
878-880 |
IN |
denotes |
in |
| T346 |
881-884 |
DT |
denotes |
the |
| T347 |
892-899 |
NN |
denotes |
cochlea |
| T348 |
885-891 |
JJ |
denotes |
mutant |
| T349 |
899-901 |
, |
denotes |
, |
| T350 |
901-913 |
VBD |
denotes |
concentrated |
| T351 |
914-916 |
IN |
denotes |
in |
| T352 |
917-920 |
DT |
denotes |
the |
| T353 |
921-925 |
NN |
denotes |
base |
| T354 |
925-926 |
. |
denotes |
. |
| T355 |
926-1010 |
sentence |
denotes |
Around 20% of immature hair cells exist in the mutant vestibular sensory epithelia. |
| T356 |
927-933 |
IN |
denotes |
Around |
| T357 |
934-936 |
CD |
denotes |
20 |
| T358 |
936-937 |
NN |
denotes |
% |
| T359 |
961-966 |
VBP |
denotes |
exist |
| T360 |
938-940 |
IN |
denotes |
of |
| T361 |
941-949 |
JJ |
denotes |
immature |
| T362 |
955-960 |
NNS |
denotes |
cells |
| T363 |
950-954 |
NN |
denotes |
hair |
| T364 |
967-969 |
IN |
denotes |
in |
| T365 |
970-973 |
DT |
denotes |
the |
| T366 |
1000-1009 |
NNS |
denotes |
epithelia |
| T367 |
974-980 |
JJ |
denotes |
mutant |
| T368 |
981-991 |
JJ |
denotes |
vestibular |
| T369 |
992-999 |
JJ |
denotes |
sensory |
| T370 |
1009-1010 |
. |
denotes |
. |
| T371 |
1010-1166 |
sentence |
denotes |
Despite more severe loss of hair cells (1% compared to 20%), the cochlea retains many more sensory neurons (46% compared to 15%) than vestibular epithelia. |
| T372 |
1011-1018 |
IN |
denotes |
Despite |
| T373 |
1084-1091 |
VBZ |
denotes |
retains |
| T374 |
1019-1023 |
RBR |
denotes |
more |
| T375 |
1024-1030 |
JJ |
denotes |
severe |
| T376 |
1031-1035 |
NN |
denotes |
loss |
| T377 |
1036-1038 |
IN |
denotes |
of |
| T378 |
1039-1043 |
NN |
denotes |
hair |
| T379 |
1044-1049 |
NNS |
denotes |
cells |
| T380 |
1050-1051 |
-LRB- |
denotes |
( |
| T381 |
1052-1053 |
NN |
denotes |
% |
| T382 |
1051-1052 |
CD |
denotes |
1 |
| T383 |
1054-1062 |
VBN |
denotes |
compared |
| T384 |
1063-1065 |
IN |
denotes |
to |
| T385 |
1066-1068 |
CD |
denotes |
20 |
| T386 |
1068-1069 |
NN |
denotes |
% |
| T387 |
1069-1070 |
-RRB- |
denotes |
) |
| T388 |
1070-1072 |
, |
denotes |
, |
| T389 |
1072-1075 |
DT |
denotes |
the |
| T390 |
1076-1083 |
NN |
denotes |
cochlea |
| T391 |
1092-1096 |
JJ |
denotes |
many |
| T392 |
1110-1117 |
NNS |
denotes |
neurons |
| T393 |
1097-1101 |
JJR |
denotes |
more |
| T394 |
1102-1109 |
JJ |
denotes |
sensory |
| T395 |
1118-1119 |
-LRB- |
denotes |
( |
| T396 |
1121-1122 |
NN |
denotes |
% |
| T397 |
1119-1121 |
CD |
denotes |
46 |
| T398 |
1123-1131 |
VBN |
denotes |
compared |
| T399 |
1132-1134 |
IN |
denotes |
to |
| T400 |
1135-1137 |
CD |
denotes |
15 |
| T401 |
1137-1138 |
NN |
denotes |
% |
| T402 |
1138-1139 |
-RRB- |
denotes |
) |
| T403 |
1140-1144 |
IN |
denotes |
than |
| T404 |
1145-1155 |
JJ |
denotes |
vestibular |
| T405 |
1156-1165 |
NNS |
denotes |
epithelia |
| T406 |
1165-1166 |
. |
denotes |
. |
| T407 |
1166-1325 |
sentence |
denotes |
Even 6 months old mutant mice have some fibers to all vestibular sensory epithelia and many more to the cochlear apex which lacks MyoVIIa positive hair cells. |
| T408 |
1167-1171 |
RB |
denotes |
Even |
| T409 |
1192-1196 |
NNS |
denotes |
mice |
| T410 |
1172-1173 |
CD |
denotes |
6 |
| T411 |
1174-1180 |
NNS |
denotes |
months |
| T412 |
1181-1184 |
JJ |
denotes |
old |
| T413 |
1185-1191 |
JJ |
denotes |
mutant |
| T414 |
1197-1201 |
VBP |
denotes |
have |
| T415 |
1202-1206 |
DT |
denotes |
some |
| T416 |
1207-1213 |
NNS |
denotes |
fibers |
| T417 |
1214-1216 |
IN |
denotes |
to |
| T418 |
1217-1220 |
DT |
denotes |
all |
| T419 |
1240-1249 |
NNS |
denotes |
epithelia |
| T420 |
1221-1231 |
JJ |
denotes |
vestibular |
| T421 |
1232-1239 |
JJ |
denotes |
sensory |
| T422 |
1250-1253 |
CC |
denotes |
and |
| T423 |
1254-1258 |
JJ |
denotes |
many |
| T424 |
1259-1263 |
JJR |
denotes |
more |
| T425 |
1264-1266 |
IN |
denotes |
to |
| T426 |
1267-1270 |
DT |
denotes |
the |
| T427 |
1280-1284 |
NN |
denotes |
apex |
| T428 |
1271-1279 |
JJ |
denotes |
cochlear |
| T429 |
1285-1290 |
WDT |
denotes |
which |
| T430 |
1291-1296 |
VBZ |
denotes |
lacks |
| T431 |
1297-1304 |
NN |
denotes |
MyoVIIa |
| T432 |
1305-1313 |
JJ |
denotes |
positive |
| T433 |
1319-1324 |
NNS |
denotes |
cells |
| T434 |
1314-1318 |
NN |
denotes |
hair |
| T435 |
1324-1325 |
. |
denotes |
. |
| T436 |
1325-1488 |
sentence |
denotes |
Topologically organized central cochlea projections exist at least until P8, suggesting that functional hair cells are not required to establish such projections. |
| T437 |
1326-1339 |
RB |
denotes |
Topologically |
| T438 |
1340-1349 |
VBN |
denotes |
organized |
| T439 |
1366-1377 |
NNS |
denotes |
projections |
| T440 |
1350-1357 |
JJ |
denotes |
central |
| T441 |
1358-1365 |
NN |
denotes |
cochlea |
| T442 |
1378-1383 |
VBP |
denotes |
exist |
| T443 |
1384-1386 |
RB |
denotes |
at |
| T444 |
1387-1392 |
RBS |
denotes |
least |
| T445 |
1393-1398 |
IN |
denotes |
until |
| T446 |
1399-1401 |
NN |
denotes |
P8 |
| T447 |
1401-1403 |
, |
denotes |
, |
| T448 |
1403-1413 |
VBG |
denotes |
suggesting |
| T449 |
1414-1418 |
IN |
denotes |
that |
| T450 |
1449-1457 |
VBN |
denotes |
required |
| T451 |
1419-1429 |
JJ |
denotes |
functional |
| T452 |
1435-1440 |
NNS |
denotes |
cells |
| T453 |
1430-1434 |
NN |
denotes |
hair |
| T454 |
1441-1444 |
VBP |
denotes |
are |
| T455 |
1445-1448 |
RB |
denotes |
not |
| T456 |
1458-1460 |
TO |
denotes |
to |
| T457 |
1461-1470 |
VB |
denotes |
establish |
| T458 |
1471-1475 |
JJ |
denotes |
such |
| T459 |
1476-1487 |
NNS |
denotes |
projections |
| T460 |
1487-1488 |
. |
denotes |
. |
| T461 |
1488-1500 |
sentence |
denotes |
Conclusion |
| T462 |
1490-1500 |
NN |
denotes |
Conclusion |
| T463 |
1500-1658 |
sentence |
denotes |
The limited expression of neurotrophins in the cochlea of Brn3c null mice suffices to support many sensory neurons, particularly in the cochlea, until birth. |
| T464 |
1501-1504 |
DT |
denotes |
The |
| T465 |
1513-1523 |
NN |
denotes |
expression |
| T466 |
1505-1512 |
VBN |
denotes |
limited |
| T467 |
1575-1583 |
VBZ |
denotes |
suffices |
| T468 |
1524-1526 |
IN |
denotes |
of |
| T469 |
1527-1540 |
NNS |
denotes |
neurotrophins |
| T470 |
1541-1543 |
IN |
denotes |
in |
| T471 |
1544-1547 |
DT |
denotes |
the |
| T472 |
1548-1555 |
NN |
denotes |
cochlea |
| T473 |
1556-1558 |
IN |
denotes |
of |
| T474 |
1559-1564 |
NN |
denotes |
Brn3c |
| T475 |
1565-1569 |
JJ |
denotes |
null |
| T476 |
1570-1574 |
NNS |
denotes |
mice |
| T477 |
1584-1586 |
TO |
denotes |
to |
| T478 |
1587-1594 |
VB |
denotes |
support |
| T479 |
1595-1599 |
JJ |
denotes |
many |
| T480 |
1608-1615 |
NNS |
denotes |
neurons |
| T481 |
1600-1607 |
JJ |
denotes |
sensory |
| T482 |
1615-1617 |
, |
denotes |
, |
| T483 |
1617-1629 |
RB |
denotes |
particularly |
| T484 |
1630-1632 |
IN |
denotes |
in |
| T485 |
1633-1636 |
DT |
denotes |
the |
| T486 |
1637-1644 |
NN |
denotes |
cochlea |
| T487 |
1644-1646 |
, |
denotes |
, |
| T488 |
1646-1651 |
IN |
denotes |
until |
| T489 |
1652-1657 |
NN |
denotes |
birth |
| T490 |
1657-1658 |
. |
denotes |
. |
| T491 |
1658-1747 |
sentence |
denotes |
The molecular nature of the long term survival of apical spiral neurons remains unclear. |
| T492 |
1659-1662 |
DT |
denotes |
The |
| T493 |
1673-1679 |
NN |
denotes |
nature |
| T494 |
1663-1672 |
JJ |
denotes |
molecular |
| T495 |
1731-1738 |
VBZ |
denotes |
remains |
| T496 |
1680-1682 |
IN |
denotes |
of |
| T497 |
1683-1686 |
DT |
denotes |
the |
| T498 |
1697-1705 |
NN |
denotes |
survival |
| T499 |
1687-1691 |
JJ |
denotes |
long |
| T500 |
1692-1696 |
NN |
denotes |
term |
| T501 |
1706-1708 |
IN |
denotes |
of |
| T502 |
1709-1715 |
JJ |
denotes |
apical |
| T503 |
1716-1722 |
JJ |
denotes |
spiral |
| T504 |
1723-1730 |
NNS |
denotes |
neurons |
| T505 |
1739-1746 |
JJ |
denotes |
unclear |
| T506 |
1746-1747 |
. |
denotes |
. |
| T820 |
1760-1765 |
NN |
denotes |
Brn3c |
| T821 |
1766-1768 |
VBZ |
denotes |
is |
| T822 |
1769-1770 |
DT |
denotes |
a |
| T823 |
1782-1788 |
NN |
denotes |
factor |
| T824 |
1771-1774 |
NN |
denotes |
POU |
| T825 |
1775-1781 |
NN |
denotes |
domain |
| T826 |
1789-1793 |
WDT |
denotes |
that |
| T827 |
1794-1796 |
VBZ |
denotes |
is |
| T828 |
1797-1804 |
JJ |
denotes |
crucial |
| T829 |
1805-1808 |
IN |
denotes |
for |
| T830 |
1809-1814 |
JJ |
denotes |
inner |
| T831 |
1815-1818 |
JJ |
denotes |
ear |
| T832 |
1829-1840 |
NN |
denotes |
development |
| T833 |
1819-1823 |
NN |
denotes |
hair |
| T834 |
1824-1828 |
NN |
denotes |
cell |
| T835 |
1840-1841 |
. |
denotes |
. |
| T836 |
1841-1902 |
sentence |
denotes |
Targeted null Brn3c mutants have no mature hair cells [1,2]. |
| T837 |
1842-1850 |
VBN |
denotes |
Targeted |
| T838 |
1862-1869 |
NNS |
denotes |
mutants |
| T839 |
1851-1855 |
JJ |
denotes |
null |
| T840 |
1856-1861 |
NN |
denotes |
Brn3c |
| T841 |
1870-1874 |
VBP |
denotes |
have |
| T842 |
1875-1877 |
DT |
denotes |
no |
| T843 |
1890-1895 |
NNS |
denotes |
cells |
| T844 |
1878-1884 |
JJ |
denotes |
mature |
| T845 |
1885-1889 |
NN |
denotes |
hair |
| T846 |
1896-1897 |
-LRB- |
denotes |
[ |
| T847 |
1899-1900 |
CD |
denotes |
2 |
| T848 |
1897-1898 |
CD |
denotes |
1 |
| T849 |
1898-1899 |
, |
denotes |
, |
| T850 |
1900-1901 |
-RRB- |
denotes |
] |
| T851 |
1901-1902 |
. |
denotes |
. |
| T852 |
1902-2081 |
sentence |
denotes |
Close examination has revealed that some 'immature' hair cells form in Brn3c null mutants and express cellular markers such as Myosin VI and VIIa, calretinin and parvalbumin [3]. |
| T853 |
1903-1908 |
NN |
denotes |
Close |
| T854 |
1909-1920 |
NN |
denotes |
examination |
| T855 |
1925-1933 |
VBN |
denotes |
revealed |
| T856 |
1921-1924 |
VBZ |
denotes |
has |
| T857 |
1934-1938 |
IN |
denotes |
that |
| T858 |
1966-1970 |
VBP |
denotes |
form |
| T859 |
1939-1943 |
DT |
denotes |
some |
| T860 |
1960-1965 |
NNS |
denotes |
cells |
| T861 |
1944-1945 |
`` |
denotes |
' |
| T862 |
1945-1953 |
JJ |
denotes |
immature |
| T863 |
1953-1954 |
'' |
denotes |
' |
| T864 |
1955-1959 |
NN |
denotes |
hair |
| T865 |
1971-1973 |
IN |
denotes |
in |
| T866 |
1974-1979 |
NN |
denotes |
Brn3c |
| T867 |
1985-1992 |
NNS |
denotes |
mutants |
| T868 |
1980-1984 |
JJ |
denotes |
null |
| T869 |
1993-1996 |
CC |
denotes |
and |
| T870 |
1997-2004 |
VBP |
denotes |
express |
| T871 |
2005-2013 |
JJ |
denotes |
cellular |
| T872 |
2014-2021 |
NNS |
denotes |
markers |
| T873 |
2022-2026 |
JJ |
denotes |
such |
| T874 |
2027-2029 |
IN |
denotes |
as |
| T875 |
2030-2036 |
NN |
denotes |
Myosin |
| T876 |
2037-2039 |
NN |
denotes |
VI |
| T877 |
2040-2043 |
CC |
denotes |
and |
| T878 |
2044-2048 |
NN |
denotes |
VIIa |
| T879 |
2048-2050 |
, |
denotes |
, |
| T880 |
2050-2060 |
NN |
denotes |
calretinin |
| T881 |
2061-2064 |
CC |
denotes |
and |
| T882 |
2065-2076 |
NN |
denotes |
parvalbumin |
| T883 |
2077-2078 |
-LRB- |
denotes |
[ |
| T884 |
2078-2079 |
CD |
denotes |
3 |
| T885 |
2079-2080 |
-RRB- |
denotes |
] |
| T886 |
2080-2081 |
. |
denotes |
. |
| T887 |
2081-2177 |
sentence |
denotes |
Furthermore, these immature hair cells of Brn3c null mutants undergo apoptosis in neonates [3]. |
| T888 |
2082-2093 |
RB |
denotes |
Furthermore |
| T889 |
2143-2150 |
VBP |
denotes |
undergo |
| T890 |
2093-2095 |
, |
denotes |
, |
| T891 |
2095-2100 |
DT |
denotes |
these |
| T892 |
2115-2120 |
NNS |
denotes |
cells |
| T893 |
2101-2109 |
JJ |
denotes |
immature |
| T894 |
2110-2114 |
NN |
denotes |
hair |
| T895 |
2121-2123 |
IN |
denotes |
of |
| T896 |
2124-2129 |
NN |
denotes |
Brn3c |
| T897 |
2135-2142 |
NNS |
denotes |
mutants |
| T898 |
2130-2134 |
JJ |
denotes |
null |
| T899 |
2151-2160 |
NN |
denotes |
apoptosis |
| T900 |
2161-2163 |
IN |
denotes |
in |
| T901 |
2164-2172 |
NNS |
denotes |
neonates |
| T902 |
2173-2174 |
-LRB- |
denotes |
[ |
| T903 |
2174-2175 |
CD |
denotes |
3 |
| T904 |
2175-2176 |
-RRB- |
denotes |
] |
| T905 |
2176-2177 |
. |
denotes |
. |
| T906 |
2177-2350 |
sentence |
denotes |
Consistent with an apparent absence of mature hair cells, initial work suggested that all vestibular and most spiral ganglion cells are lost by postnatal day 14 (P14; [1]). |
| T907 |
2178-2188 |
JJ |
denotes |
Consistent |
| T908 |
2249-2258 |
VBD |
denotes |
suggested |
| T909 |
2189-2193 |
IN |
denotes |
with |
| T910 |
2194-2196 |
DT |
denotes |
an |
| T911 |
2206-2213 |
NN |
denotes |
absence |
| T912 |
2197-2205 |
JJ |
denotes |
apparent |
| T913 |
2214-2216 |
IN |
denotes |
of |
| T914 |
2217-2223 |
JJ |
denotes |
mature |
| T915 |
2229-2234 |
NNS |
denotes |
cells |
| T916 |
2224-2228 |
NN |
denotes |
hair |
| T917 |
2234-2236 |
, |
denotes |
, |
| T918 |
2236-2243 |
JJ |
denotes |
initial |
| T919 |
2244-2248 |
NN |
denotes |
work |
| T920 |
2259-2263 |
IN |
denotes |
that |
| T921 |
2314-2318 |
VBN |
denotes |
lost |
| T922 |
2264-2267 |
DT |
denotes |
all |
| T923 |
2268-2278 |
JJ |
denotes |
vestibular |
| T924 |
2279-2282 |
CC |
denotes |
and |
| T925 |
2283-2287 |
DT |
denotes |
most |
| T926 |
2288-2294 |
JJ |
denotes |
spiral |
| T927 |
2304-2309 |
NNS |
denotes |
cells |
| T928 |
2295-2303 |
NN |
denotes |
ganglion |
| T929 |
2310-2313 |
VBP |
denotes |
are |
| T930 |
2319-2321 |
IN |
denotes |
by |
| T931 |
2322-2331 |
JJ |
denotes |
postnatal |
| T932 |
2332-2335 |
NN |
denotes |
day |
| T933 |
2336-2338 |
CD |
denotes |
14 |
| T934 |
2339-2340 |
-LRB- |
denotes |
( |
| T935 |
2340-2343 |
NN |
denotes |
P14 |
| T936 |
2343-2344 |
: |
denotes |
; |
| T937 |
2345-2346 |
-LRB- |
denotes |
[ |
| T938 |
2346-2347 |
CD |
denotes |
1 |
| T939 |
2347-2348 |
-RRB- |
denotes |
] |
| T940 |
2348-2349 |
-RRB- |
denotes |
) |
| T941 |
2349-2350 |
. |
denotes |
. |
| T942 |
2350-2495 |
sentence |
denotes |
However, more detailed quantification by others [3] reported that at P4 about 77% of vestibular neurons and only 29% of spiral neurons are lost. |
| T943 |
2351-2358 |
RB |
denotes |
However |
| T944 |
2403-2411 |
VBD |
denotes |
reported |
| T945 |
2358-2360 |
, |
denotes |
, |
| T946 |
2360-2364 |
RBR |
denotes |
more |
| T947 |
2374-2388 |
NN |
denotes |
quantification |
| T948 |
2365-2373 |
JJ |
denotes |
detailed |
| T949 |
2389-2391 |
IN |
denotes |
by |
| T950 |
2392-2398 |
NNS |
denotes |
others |
| T951 |
2399-2400 |
-LRB- |
denotes |
[ |
| T952 |
2400-2401 |
CD |
denotes |
3 |
| T953 |
2401-2402 |
-RRB- |
denotes |
] |
| T954 |
2412-2416 |
IN |
denotes |
that |
| T955 |
2490-2494 |
VBN |
denotes |
lost |
| T956 |
2417-2419 |
IN |
denotes |
at |
| T957 |
2420-2422 |
NN |
denotes |
P4 |
| T958 |
2423-2428 |
IN |
denotes |
about |
| T959 |
2429-2431 |
CD |
denotes |
77 |
| T960 |
2431-2432 |
NN |
denotes |
% |
| T961 |
2433-2435 |
IN |
denotes |
of |
| T962 |
2436-2446 |
JJ |
denotes |
vestibular |
| T963 |
2447-2454 |
NNS |
denotes |
neurons |
| T964 |
2455-2458 |
CC |
denotes |
and |
| T965 |
2459-2463 |
RB |
denotes |
only |
| T966 |
2464-2466 |
CD |
denotes |
29 |
| T967 |
2466-2467 |
NN |
denotes |
% |
| T968 |
2468-2470 |
IN |
denotes |
of |
| T969 |
2471-2477 |
JJ |
denotes |
spiral |
| T970 |
2478-2485 |
NNS |
denotes |
neurons |
| T971 |
2486-2489 |
VBP |
denotes |
are |
| T972 |
2494-2495 |
. |
denotes |
. |
| T973 |
2495-2566 |
sentence |
denotes |
It was suggested that there is possibly a complete loss in adults [3]. |
| T974 |
2496-2498 |
PRP |
denotes |
It |
| T975 |
2503-2512 |
VBN |
denotes |
suggested |
| T976 |
2499-2502 |
VBD |
denotes |
was |
| T977 |
2513-2517 |
IN |
denotes |
that |
| T978 |
2524-2526 |
VBZ |
denotes |
is |
| T979 |
2518-2523 |
EX |
denotes |
there |
| T980 |
2527-2535 |
RB |
denotes |
possibly |
| T981 |
2536-2537 |
DT |
denotes |
a |
| T982 |
2547-2551 |
NN |
denotes |
loss |
| T983 |
2538-2546 |
JJ |
denotes |
complete |
| T984 |
2552-2554 |
IN |
denotes |
in |
| T985 |
2555-2561 |
NNS |
denotes |
adults |
| T986 |
2562-2563 |
-LRB- |
denotes |
[ |
| T987 |
2563-2564 |
CD |
denotes |
3 |
| T988 |
2564-2565 |
-RRB- |
denotes |
] |
| T989 |
2565-2566 |
. |
denotes |
. |
| T990 |
2566-2700 |
sentence |
denotes |
Other than these preliminary statements, no data exists concerning the detailed pattern of loss of innervation in Brn3c null mutants. |
| T991 |
2567-2572 |
RB |
denotes |
Other |
| T992 |
2616-2622 |
VBZ |
denotes |
exists |
| T993 |
2573-2577 |
IN |
denotes |
than |
| T994 |
2578-2583 |
DT |
denotes |
these |
| T995 |
2596-2606 |
NNS |
denotes |
statements |
| T996 |
2584-2595 |
JJ |
denotes |
preliminary |
| T997 |
2606-2608 |
, |
denotes |
, |
| T998 |
2608-2610 |
DT |
denotes |
no |
| T999 |
2611-2615 |
NN |
denotes |
data |
| T1000 |
2623-2633 |
VBG |
denotes |
concerning |
| T1001 |
2634-2637 |
DT |
denotes |
the |
| T1002 |
2647-2654 |
NN |
denotes |
pattern |
| T1003 |
2638-2646 |
JJ |
denotes |
detailed |
| T1004 |
2655-2657 |
IN |
denotes |
of |
| T1005 |
2658-2662 |
NN |
denotes |
loss |
| T1006 |
2663-2665 |
IN |
denotes |
of |
| T1007 |
2666-2677 |
NN |
denotes |
innervation |
| T1008 |
2678-2680 |
IN |
denotes |
in |
| T1009 |
2681-2686 |
NN |
denotes |
Brn3c |
| T1010 |
2687-2691 |
JJ |
denotes |
null |
| T1011 |
2692-2699 |
NNS |
denotes |
mutants |
| T1012 |
2699-2700 |
. |
denotes |
. |
| T1013 |
2700-2919 |
sentence |
denotes |
The initial development and partial differentiation of hair cells in Brn3c mutants could possibly lead to some neurotrophin expression in these cells to sustain sensory neurons through embryonic development and beyond. |
| T1014 |
2701-2704 |
DT |
denotes |
The |
| T1015 |
2713-2724 |
NN |
denotes |
development |
| T1016 |
2705-2712 |
JJ |
denotes |
initial |
| T1017 |
2799-2803 |
VB |
denotes |
lead |
| T1018 |
2725-2728 |
CC |
denotes |
and |
| T1019 |
2729-2736 |
JJ |
denotes |
partial |
| T1020 |
2737-2752 |
NN |
denotes |
differentiation |
| T1021 |
2753-2755 |
IN |
denotes |
of |
| T1022 |
2756-2760 |
NN |
denotes |
hair |
| T1023 |
2761-2766 |
NNS |
denotes |
cells |
| T1024 |
2767-2769 |
IN |
denotes |
in |
| T1025 |
2770-2775 |
NN |
denotes |
Brn3c |
| T1026 |
2776-2783 |
NNS |
denotes |
mutants |
| T1027 |
2784-2789 |
MD |
denotes |
could |
| T1028 |
2790-2798 |
RB |
denotes |
possibly |
| T1029 |
2804-2806 |
IN |
denotes |
to |
| T1030 |
2807-2811 |
DT |
denotes |
some |
| T1031 |
2825-2835 |
NN |
denotes |
expression |
| T1032 |
2812-2824 |
NN |
denotes |
neurotrophin |
| T1033 |
2836-2838 |
IN |
denotes |
in |
| T1034 |
2839-2844 |
DT |
denotes |
these |
| T1035 |
2845-2850 |
NNS |
denotes |
cells |
| T1036 |
2851-2853 |
TO |
denotes |
to |
| T1037 |
2854-2861 |
VB |
denotes |
sustain |
| T1038 |
2862-2869 |
JJ |
denotes |
sensory |
| T1039 |
2870-2877 |
NNS |
denotes |
neurons |
| T1040 |
2878-2885 |
IN |
denotes |
through |
| T1041 |
2886-2895 |
JJ |
denotes |
embryonic |
| T1042 |
2896-2907 |
NN |
denotes |
development |
| T1043 |
2908-2911 |
CC |
denotes |
and |
| T1044 |
2912-2918 |
RB |
denotes |
beyond |
| T1045 |
2918-2919 |
. |
denotes |
. |
| T1046 |
2919-3119 |
sentence |
denotes |
Data on various single and compound neurotrophin null mutants have shown that the loss of a specific neurotrophin leads to topologically restricted loss of sensory neurons in the embryonic ear [4,5]. |
| T1047 |
2920-2924 |
NNS |
denotes |
Data |
| T1048 |
2987-2992 |
VBN |
denotes |
shown |
| T1049 |
2925-2927 |
IN |
denotes |
on |
| T1050 |
2928-2935 |
JJ |
denotes |
various |
| T1051 |
2974-2981 |
NNS |
denotes |
mutants |
| T1052 |
2936-2942 |
JJ |
denotes |
single |
| T1053 |
2956-2968 |
NN |
denotes |
neurotrophin |
| T1054 |
2943-2946 |
CC |
denotes |
and |
| T1055 |
2947-2955 |
NN |
denotes |
compound |
| T1056 |
2969-2973 |
JJ |
denotes |
null |
| T1057 |
2982-2986 |
VBP |
denotes |
have |
| T1058 |
2993-2997 |
IN |
denotes |
that |
| T1059 |
3034-3039 |
VBZ |
denotes |
leads |
| T1060 |
2998-3001 |
DT |
denotes |
the |
| T1061 |
3002-3006 |
NN |
denotes |
loss |
| T1062 |
3007-3009 |
IN |
denotes |
of |
| T1063 |
3010-3011 |
DT |
denotes |
a |
| T1064 |
3021-3033 |
NN |
denotes |
neurotrophin |
| T1065 |
3012-3020 |
JJ |
denotes |
specific |
| T1066 |
3040-3042 |
IN |
denotes |
to |
| T1067 |
3043-3056 |
RB |
denotes |
topologically |
| T1068 |
3057-3067 |
JJ |
denotes |
restricted |
| T1069 |
3068-3072 |
NN |
denotes |
loss |
| T1070 |
3073-3075 |
IN |
denotes |
of |
| T1071 |
3076-3083 |
JJ |
denotes |
sensory |
| T1072 |
3084-3091 |
NNS |
denotes |
neurons |
| T1073 |
3092-3094 |
IN |
denotes |
in |
| T1074 |
3095-3098 |
DT |
denotes |
the |
| T1075 |
3109-3112 |
NN |
denotes |
ear |
| T1076 |
3099-3108 |
JJ |
denotes |
embryonic |
| T1077 |
3113-3114 |
-LRB- |
denotes |
[ |
| T1078 |
3116-3117 |
CD |
denotes |
5 |
| T1079 |
3114-3115 |
CD |
denotes |
4 |
| T1080 |
3115-3116 |
, |
denotes |
, |
| T1081 |
3117-3118 |
-RRB- |
denotes |
] |
| T1082 |
3118-3119 |
. |
denotes |
. |
| T1083 |
3119-3247 |
sentence |
denotes |
Such selective loss in Brn3c null mutants would therefore indicate reduction of a specific neurotrophin in immature hair cells. |
| T1084 |
3120-3124 |
JJ |
denotes |
Such |
| T1085 |
3135-3139 |
NN |
denotes |
loss |
| T1086 |
3125-3134 |
JJ |
denotes |
selective |
| T1087 |
3178-3186 |
VB |
denotes |
indicate |
| T1088 |
3140-3142 |
IN |
denotes |
in |
| T1089 |
3143-3148 |
NN |
denotes |
Brn3c |
| T1090 |
3154-3161 |
NNS |
denotes |
mutants |
| T1091 |
3149-3153 |
JJ |
denotes |
null |
| T1092 |
3162-3167 |
MD |
denotes |
would |
| T1093 |
3168-3177 |
RB |
denotes |
therefore |
| T1094 |
3187-3196 |
NN |
denotes |
reduction |
| T1095 |
3197-3199 |
IN |
denotes |
of |
| T1096 |
3200-3201 |
DT |
denotes |
a |
| T1097 |
3211-3223 |
NN |
denotes |
neurotrophin |
| T1098 |
3202-3210 |
JJ |
denotes |
specific |
| T1099 |
3224-3226 |
IN |
denotes |
in |
| T1100 |
3227-3235 |
JJ |
denotes |
immature |
| T1101 |
3241-3246 |
NNS |
denotes |
cells |
| T1102 |
3236-3240 |
NN |
denotes |
hair |
| T1103 |
3246-3247 |
. |
denotes |
. |
| T1104 |
3247-3388 |
sentence |
denotes |
Moreover, recent work shows that in embryos NT-3 is primarily expressed in supporting cells, moving only around birth into hair cells [6,7]. |
| T1105 |
3248-3256 |
RB |
denotes |
Moreover |
| T1106 |
3270-3275 |
VBZ |
denotes |
shows |
| T1107 |
3256-3258 |
, |
denotes |
, |
| T1108 |
3258-3264 |
JJ |
denotes |
recent |
| T1109 |
3265-3269 |
NN |
denotes |
work |
| T1110 |
3276-3280 |
IN |
denotes |
that |
| T1111 |
3310-3319 |
VBN |
denotes |
expressed |
| T1112 |
3281-3283 |
IN |
denotes |
in |
| T1113 |
3284-3291 |
NNS |
denotes |
embryos |
| T1114 |
3292-3294 |
NN |
denotes |
NT |
| T1115 |
3294-3295 |
HYPH |
denotes |
- |
| T1116 |
3295-3296 |
CD |
denotes |
3 |
| T1117 |
3297-3299 |
VBZ |
denotes |
is |
| T1118 |
3300-3309 |
RB |
denotes |
primarily |
| T1119 |
3320-3322 |
IN |
denotes |
in |
| T1120 |
3323-3333 |
VBG |
denotes |
supporting |
| T1121 |
3334-3339 |
NNS |
denotes |
cells |
| T1122 |
3339-3341 |
, |
denotes |
, |
| T1123 |
3341-3347 |
VBG |
denotes |
moving |
| T1124 |
3348-3352 |
RB |
denotes |
only |
| T1125 |
3353-3359 |
IN |
denotes |
around |
| T1126 |
3360-3365 |
NN |
denotes |
birth |
| T1127 |
3366-3370 |
IN |
denotes |
into |
| T1128 |
3371-3375 |
NN |
denotes |
hair |
| T1129 |
3376-3381 |
NNS |
denotes |
cells |
| T1130 |
3382-3383 |
-LRB- |
denotes |
[ |
| T1131 |
3385-3386 |
CD |
denotes |
7 |
| T1132 |
3383-3384 |
CD |
denotes |
6 |
| T1133 |
3384-3385 |
, |
denotes |
, |
| T1134 |
3386-3387 |
-RRB- |
denotes |
] |
| T1135 |
3387-3388 |
. |
denotes |
. |
| T1136 |
3388-3666 |
sentence |
denotes |
In fact, the selective loss of vestibular as compared to cochlear sensory neurons (77% versus 29%; [3]) suggests that NT-3 expression may be less downregulated in Brn3c null mutants than BDNF [6,8,9], provided that at least some differentiation of supporting cells takes place. |
| T1137 |
3389-3391 |
IN |
denotes |
In |
| T1138 |
3493-3501 |
VBZ |
denotes |
suggests |
| T1139 |
3392-3396 |
NN |
denotes |
fact |
| T1140 |
3396-3398 |
, |
denotes |
, |
| T1141 |
3398-3401 |
DT |
denotes |
the |
| T1142 |
3412-3416 |
NN |
denotes |
loss |
| T1143 |
3402-3411 |
JJ |
denotes |
selective |
| T1144 |
3417-3419 |
IN |
denotes |
of |
| T1145 |
3420-3430 |
JJ |
denotes |
vestibular |
| T1146 |
3431-3433 |
IN |
denotes |
as |
| T1147 |
3434-3442 |
VBN |
denotes |
compared |
| T1148 |
3443-3445 |
IN |
denotes |
to |
| T1149 |
3446-3454 |
JJ |
denotes |
cochlear |
| T1150 |
3455-3462 |
JJ |
denotes |
sensory |
| T1151 |
3463-3470 |
NNS |
denotes |
neurons |
| T1152 |
3471-3472 |
-LRB- |
denotes |
( |
| T1153 |
3474-3475 |
NN |
denotes |
% |
| T1154 |
3472-3474 |
CD |
denotes |
77 |
| T1155 |
3476-3482 |
CC |
denotes |
versus |
| T1156 |
3483-3485 |
CD |
denotes |
29 |
| T1157 |
3485-3486 |
NN |
denotes |
% |
| T1158 |
3486-3487 |
: |
denotes |
; |
| T1159 |
3488-3489 |
-LRB- |
denotes |
[ |
| T1160 |
3489-3490 |
CD |
denotes |
3 |
| T1161 |
3490-3491 |
-RRB- |
denotes |
] |
| T1162 |
3491-3492 |
-RRB- |
denotes |
) |
| T1163 |
3502-3506 |
IN |
denotes |
that |
| T1164 |
3527-3529 |
VB |
denotes |
be |
| T1165 |
3507-3509 |
NN |
denotes |
NT |
| T1166 |
3512-3522 |
NN |
denotes |
expression |
| T1167 |
3509-3510 |
HYPH |
denotes |
- |
| T1168 |
3510-3511 |
CD |
denotes |
3 |
| T1169 |
3523-3526 |
MD |
denotes |
may |
| T1170 |
3530-3534 |
RBR |
denotes |
less |
| T1171 |
3535-3548 |
VBN |
denotes |
downregulated |
| T1172 |
3549-3551 |
IN |
denotes |
in |
| T1173 |
3552-3557 |
NN |
denotes |
Brn3c |
| T1174 |
3563-3570 |
NNS |
denotes |
mutants |
| T1175 |
3558-3562 |
JJ |
denotes |
null |
| T1176 |
3571-3575 |
IN |
denotes |
than |
| T1177 |
3576-3580 |
NN |
denotes |
BDNF |
| T1178 |
3581-3582 |
-LRB- |
denotes |
[ |
| T1179 |
3586-3587 |
CD |
denotes |
9 |
| T1180 |
3582-3583 |
CD |
denotes |
6 |
| T1181 |
3583-3584 |
, |
denotes |
, |
| T1182 |
3584-3585 |
CD |
denotes |
8 |
| T1183 |
3585-3586 |
, |
denotes |
, |
| T1184 |
3587-3588 |
-RRB- |
denotes |
] |
| T1185 |
3588-3590 |
, |
denotes |
, |
| T1186 |
3590-3598 |
VBN |
denotes |
provided |
| T1187 |
3599-3603 |
IN |
denotes |
that |
| T1188 |
3654-3659 |
VBZ |
denotes |
takes |
| T1189 |
3604-3606 |
RB |
denotes |
at |
| T1190 |
3607-3612 |
RBS |
denotes |
least |
| T1191 |
3618-3633 |
NN |
denotes |
differentiation |
| T1192 |
3613-3617 |
DT |
denotes |
some |
| T1193 |
3634-3636 |
IN |
denotes |
of |
| T1194 |
3637-3647 |
VBG |
denotes |
supporting |
| T1195 |
3648-3653 |
NNS |
denotes |
cells |
| T1196 |
3660-3665 |
NN |
denotes |
place |
| T1197 |
3665-3666 |
. |
denotes |
. |
| T1198 |
3666-3819 |
sentence |
denotes |
In the ear [4] as well as elsewhere [10] neurotrophins are progressively downregulated in postnatal mammals and possibly replaced by other factors [11]. |
| T1199 |
3667-3669 |
IN |
denotes |
In |
| T1200 |
3740-3753 |
VBN |
denotes |
downregulated |
| T1201 |
3670-3673 |
DT |
denotes |
the |
| T1202 |
3674-3677 |
NN |
denotes |
ear |
| T1203 |
3678-3679 |
-LRB- |
denotes |
[ |
| T1204 |
3679-3680 |
CD |
denotes |
4 |
| T1205 |
3680-3681 |
-RRB- |
denotes |
] |
| T1206 |
3682-3684 |
RB |
denotes |
as |
| T1207 |
3690-3692 |
IN |
denotes |
as |
| T1208 |
3685-3689 |
RB |
denotes |
well |
| T1209 |
3693-3702 |
RB |
denotes |
elsewhere |
| T1210 |
3704-3706 |
CD |
denotes |
10 |
| T1211 |
3703-3704 |
-LRB- |
denotes |
[ |
| T1212 |
3706-3707 |
-RRB- |
denotes |
] |
| T1213 |
3708-3721 |
NNS |
denotes |
neurotrophins |
| T1214 |
3722-3725 |
VBP |
denotes |
are |
| T1215 |
3726-3739 |
RB |
denotes |
progressively |
| T1216 |
3754-3756 |
IN |
denotes |
in |
| T1217 |
3757-3766 |
JJ |
denotes |
postnatal |
| T1218 |
3767-3774 |
NNS |
denotes |
mammals |
| T1219 |
3775-3778 |
CC |
denotes |
and |
| T1220 |
3779-3787 |
RB |
denotes |
possibly |
| T1221 |
3788-3796 |
VBN |
denotes |
replaced |
| T1222 |
3797-3799 |
IN |
denotes |
by |
| T1223 |
3800-3805 |
JJ |
denotes |
other |
| T1224 |
3806-3813 |
NNS |
denotes |
factors |
| T1225 |
3814-3815 |
-LRB- |
denotes |
[ |
| T1226 |
3815-3817 |
CD |
denotes |
11 |
| T1227 |
3817-3818 |
-RRB- |
denotes |
] |
| T1228 |
3818-3819 |
. |
denotes |
. |
| T1229 |
3819-3943 |
sentence |
denotes |
We have investigated in detail the pattern of innervation in the Brn3c mutants, as well as the expression of NT-3 and BDNF. |
| T1230 |
3820-3822 |
PRP |
denotes |
We |
| T1231 |
3828-3840 |
VBN |
denotes |
investigated |
| T1232 |
3823-3827 |
VBP |
denotes |
have |
| T1233 |
3841-3843 |
IN |
denotes |
in |
| T1234 |
3844-3850 |
NN |
denotes |
detail |
| T1235 |
3851-3854 |
DT |
denotes |
the |
| T1236 |
3855-3862 |
NN |
denotes |
pattern |
| T1237 |
3863-3865 |
IN |
denotes |
of |
| T1238 |
3866-3877 |
NN |
denotes |
innervation |
| T1239 |
3878-3880 |
IN |
denotes |
in |
| T1240 |
3881-3884 |
DT |
denotes |
the |
| T1241 |
3891-3898 |
NNS |
denotes |
mutants |
| T1242 |
3885-3890 |
NN |
denotes |
Brn3c |
| T1243 |
3898-3900 |
, |
denotes |
, |
| T1244 |
3900-3902 |
RB |
denotes |
as |
| T1245 |
3908-3910 |
IN |
denotes |
as |
| T1246 |
3903-3907 |
RB |
denotes |
well |
| T1247 |
3911-3914 |
DT |
denotes |
the |
| T1248 |
3915-3925 |
NN |
denotes |
expression |
| T1249 |
3926-3928 |
IN |
denotes |
of |
| T1250 |
3929-3931 |
NN |
denotes |
NT |
| T1251 |
3931-3932 |
HYPH |
denotes |
- |
| T1252 |
3932-3933 |
CD |
denotes |
3 |
| T1253 |
3934-3937 |
CC |
denotes |
and |
| T1254 |
3938-3942 |
NN |
denotes |
BDNF |
| T1255 |
3942-3943 |
. |
denotes |
. |
| T1256 |
3943-4134 |
sentence |
denotes |
We want to evaluate a possible correlation between the topology of sensory neuron loss and absence of a specific neurotrophin or topological loss of hair cells at birth and in older animals. |
| T1257 |
3944-3946 |
PRP |
denotes |
We |
| T1258 |
3947-3951 |
VBP |
denotes |
want |
| T1259 |
3952-3954 |
TO |
denotes |
to |
| T1260 |
3955-3963 |
VB |
denotes |
evaluate |
| T1261 |
3964-3965 |
DT |
denotes |
a |
| T1262 |
3975-3986 |
NN |
denotes |
correlation |
| T1263 |
3966-3974 |
JJ |
denotes |
possible |
| T1264 |
3987-3994 |
IN |
denotes |
between |
| T1265 |
3995-3998 |
DT |
denotes |
the |
| T1266 |
3999-4007 |
NN |
denotes |
topology |
| T1267 |
4008-4010 |
IN |
denotes |
of |
| T1268 |
4011-4018 |
JJ |
denotes |
sensory |
| T1269 |
4019-4025 |
NN |
denotes |
neuron |
| T1270 |
4026-4030 |
NN |
denotes |
loss |
| T1271 |
4031-4034 |
CC |
denotes |
and |
| T1272 |
4035-4042 |
NN |
denotes |
absence |
| T1273 |
4043-4045 |
IN |
denotes |
of |
| T1274 |
4046-4047 |
DT |
denotes |
a |
| T1275 |
4057-4069 |
NN |
denotes |
neurotrophin |
| T1276 |
4048-4056 |
JJ |
denotes |
specific |
| T1277 |
4070-4072 |
CC |
denotes |
or |
| T1278 |
4073-4084 |
JJ |
denotes |
topological |
| T1279 |
4085-4089 |
NN |
denotes |
loss |
| T1280 |
4090-4092 |
IN |
denotes |
of |
| T1281 |
4093-4097 |
NN |
denotes |
hair |
| T1282 |
4098-4103 |
NNS |
denotes |
cells |
| T1283 |
4104-4106 |
IN |
denotes |
at |
| T1284 |
4107-4112 |
NN |
denotes |
birth |
| T1285 |
4113-4116 |
CC |
denotes |
and |
| T1286 |
4117-4119 |
IN |
denotes |
in |
| T1287 |
4120-4125 |
JJR |
denotes |
older |
| T1288 |
4126-4133 |
NNS |
denotes |
animals |
| T1289 |
4133-4134 |
. |
denotes |
. |
| T1290 |
4134-4260 |
sentence |
denotes |
This information could be important for an in-depth evaluation of the human deafness related to the Pou4f3 gene, DFNA15 [12]. |
| T1291 |
4135-4139 |
DT |
denotes |
This |
| T1292 |
4140-4151 |
NN |
denotes |
information |
| T1293 |
4158-4160 |
VB |
denotes |
be |
| T1294 |
4152-4157 |
MD |
denotes |
could |
| T1295 |
4161-4170 |
JJ |
denotes |
important |
| T1296 |
4171-4174 |
IN |
denotes |
for |
| T1297 |
4175-4177 |
DT |
denotes |
an |
| T1298 |
4187-4197 |
NN |
denotes |
evaluation |
| T1299 |
4178-4180 |
IN |
denotes |
in |
| T1300 |
4180-4181 |
HYPH |
denotes |
- |
| T1301 |
4181-4186 |
NN |
denotes |
depth |
| T1302 |
4198-4200 |
IN |
denotes |
of |
| T1303 |
4201-4204 |
DT |
denotes |
the |
| T1304 |
4211-4219 |
NN |
denotes |
deafness |
| T1305 |
4205-4210 |
JJ |
denotes |
human |
| T1306 |
4220-4227 |
VBN |
denotes |
related |
| T1307 |
4228-4230 |
IN |
denotes |
to |
| T1308 |
4231-4234 |
DT |
denotes |
the |
| T1309 |
4242-4246 |
NN |
denotes |
gene |
| T1310 |
4235-4241 |
NN |
denotes |
Pou4f3 |
| T1311 |
4246-4248 |
, |
denotes |
, |
| T1312 |
4248-4254 |
NN |
denotes |
DFNA15 |
| T1313 |
4255-4256 |
-LRB- |
denotes |
[ |
| T1314 |
4256-4258 |
CD |
denotes |
12 |
| T1315 |
4258-4259 |
-RRB- |
denotes |
] |
| T1316 |
4259-4260 |
. |
denotes |
. |
| T1317 |
4260-4409 |
sentence |
denotes |
We report here long term retention of cochlear sensory neurons for at least 6 months, in particular in the cochlear apex, in Brn3c null mutant mice. |
| T1318 |
4261-4263 |
PRP |
denotes |
We |
| T1319 |
4264-4270 |
VBP |
denotes |
report |
| T1320 |
4271-4275 |
RB |
denotes |
here |
| T1321 |
4276-4280 |
JJ |
denotes |
long |
| T1322 |
4281-4285 |
NN |
denotes |
term |
| T1323 |
4286-4295 |
NN |
denotes |
retention |
| T1324 |
4296-4298 |
IN |
denotes |
of |
| T1325 |
4299-4307 |
JJ |
denotes |
cochlear |
| T1326 |
4316-4323 |
NNS |
denotes |
neurons |
| T1327 |
4308-4315 |
JJ |
denotes |
sensory |
| T1328 |
4324-4327 |
IN |
denotes |
for |
| T1329 |
4328-4330 |
RB |
denotes |
at |
| T1330 |
4337-4338 |
CD |
denotes |
6 |
| T1331 |
4331-4336 |
RBS |
denotes |
least |
| T1332 |
4339-4345 |
NNS |
denotes |
months |
| T1333 |
4345-4347 |
, |
denotes |
, |
| T1334 |
4347-4349 |
IN |
denotes |
in |
| T1335 |
4361-4363 |
IN |
denotes |
in |
| T1336 |
4350-4360 |
JJ |
denotes |
particular |
| T1337 |
4364-4367 |
DT |
denotes |
the |
| T1338 |
4377-4381 |
NN |
denotes |
apex |
| T1339 |
4368-4376 |
JJ |
denotes |
cochlear |
| T1340 |
4381-4383 |
, |
denotes |
, |
| T1341 |
4383-4385 |
IN |
denotes |
in |
| T1342 |
4386-4391 |
NN |
denotes |
Brn3c |
| T1343 |
4392-4396 |
JJ |
denotes |
null |
| T1344 |
4404-4408 |
NNS |
denotes |
mice |
| T1345 |
4397-4403 |
JJ |
denotes |
mutant |
| T1346 |
4408-4409 |
. |
denotes |
. |
| T1347 |
4409-4628 |
sentence |
denotes |
This retention of afferents and efferents is unrelated to hair cell differentiation as not even immature hair cells can be detected at early postnatal stages with MyoVII immunocytochemistry in this part of the cochlea. |
| T1348 |
4410-4414 |
DT |
denotes |
This |
| T1349 |
4415-4424 |
NN |
denotes |
retention |
| T1350 |
4452-4454 |
VBZ |
denotes |
is |
| T1351 |
4425-4427 |
IN |
denotes |
of |
| T1352 |
4428-4437 |
NNS |
denotes |
afferents |
| T1353 |
4438-4441 |
CC |
denotes |
and |
| T1354 |
4442-4451 |
NNS |
denotes |
efferents |
| T1355 |
4455-4464 |
JJ |
denotes |
unrelated |
| T1356 |
4465-4467 |
IN |
denotes |
to |
| T1357 |
4468-4472 |
NN |
denotes |
hair |
| T1358 |
4473-4477 |
NN |
denotes |
cell |
| T1359 |
4478-4493 |
NN |
denotes |
differentiation |
| T1360 |
4494-4496 |
IN |
denotes |
as |
| T1361 |
4533-4541 |
VBN |
denotes |
detected |
| T1362 |
4497-4500 |
RB |
denotes |
not |
| T1363 |
4520-4525 |
NNS |
denotes |
cells |
| T1364 |
4501-4505 |
RB |
denotes |
even |
| T1365 |
4506-4514 |
JJ |
denotes |
immature |
| T1366 |
4515-4519 |
NN |
denotes |
hair |
| T1367 |
4526-4529 |
MD |
denotes |
can |
| T1368 |
4530-4532 |
VB |
denotes |
be |
| T1369 |
4542-4544 |
IN |
denotes |
at |
| T1370 |
4545-4550 |
JJ |
denotes |
early |
| T1371 |
4561-4567 |
NNS |
denotes |
stages |
| T1372 |
4551-4560 |
JJ |
denotes |
postnatal |
| T1373 |
4568-4572 |
IN |
denotes |
with |
| T1374 |
4573-4579 |
NN |
denotes |
MyoVII |
| T1375 |
4580-4599 |
NN |
denotes |
immunocytochemistry |
| T1376 |
4600-4602 |
IN |
denotes |
in |
| T1377 |
4603-4607 |
DT |
denotes |
this |
| T1378 |
4608-4612 |
NN |
denotes |
part |
| T1379 |
4613-4615 |
IN |
denotes |
of |
| T1380 |
4616-4619 |
DT |
denotes |
the |
| T1381 |
4620-4627 |
NN |
denotes |
cochlea |
| T1382 |
4627-4628 |
. |
denotes |
. |
| T1383 |
4628-4786 |
sentence |
denotes |
This retention of apical spiral neurons is also largely unrelated to neurotrophins which are known to be reduced in their expression in neonatal rodents [4]. |
| T1384 |
4629-4633 |
DT |
denotes |
This |
| T1385 |
4634-4643 |
NN |
denotes |
retention |
| T1386 |
4669-4671 |
VBZ |
denotes |
is |
| T1387 |
4644-4646 |
IN |
denotes |
of |
| T1388 |
4647-4653 |
JJ |
denotes |
apical |
| T1389 |
4661-4668 |
NNS |
denotes |
neurons |
| T1390 |
4654-4660 |
JJ |
denotes |
spiral |
| T1391 |
4672-4676 |
RB |
denotes |
also |
| T1392 |
4677-4684 |
RB |
denotes |
largely |
| T1393 |
4685-4694 |
JJ |
denotes |
unrelated |
| T1394 |
4695-4697 |
IN |
denotes |
to |
| T1395 |
4698-4711 |
NNS |
denotes |
neurotrophins |
| T1396 |
4712-4717 |
WDT |
denotes |
which |
| T1397 |
4722-4727 |
VBN |
denotes |
known |
| T1398 |
4718-4721 |
VBP |
denotes |
are |
| T1399 |
4728-4730 |
TO |
denotes |
to |
| T1400 |
4734-4741 |
VBN |
denotes |
reduced |
| T1401 |
4731-4733 |
VB |
denotes |
be |
| T1402 |
4742-4744 |
IN |
denotes |
in |
| T1403 |
4745-4750 |
PRP$ |
denotes |
their |
| T1404 |
4751-4761 |
NN |
denotes |
expression |
| T1405 |
4762-4764 |
IN |
denotes |
in |
| T1406 |
4765-4773 |
JJ |
denotes |
neonatal |
| T1407 |
4774-4781 |
NNS |
denotes |
rodents |
| T1408 |
4782-4783 |
-LRB- |
denotes |
[ |
| T1409 |
4783-4784 |
CD |
denotes |
4 |
| T1410 |
4784-4785 |
-RRB- |
denotes |
] |
| T1411 |
4785-4786 |
. |
denotes |
. |
| T1476 |
4796-4798 |
TO |
denotes |
To |
| T1477 |
4799-4809 |
VB |
denotes |
appreciate |
| T1478 |
4903-4907 |
VBP |
denotes |
want |
| T1479 |
4810-4813 |
DT |
denotes |
the |
| T1480 |
4814-4821 |
NNS |
denotes |
effects |
| T1481 |
4822-4824 |
IN |
denotes |
of |
| T1482 |
4825-4828 |
DT |
denotes |
the |
| T1483 |
4840-4848 |
NN |
denotes |
mutation |
| T1484 |
4829-4834 |
NN |
denotes |
Brn3c |
| T1485 |
4835-4839 |
JJ |
denotes |
null |
| T1486 |
4849-4851 |
IN |
denotes |
on |
| T1487 |
4852-4855 |
DT |
denotes |
the |
| T1488 |
4856-4863 |
NN |
denotes |
pattern |
| T1489 |
4864-4866 |
IN |
denotes |
of |
| T1490 |
4867-4870 |
DT |
denotes |
the |
| T1491 |
4881-4892 |
NN |
denotes |
innervation |
| T1492 |
4871-4876 |
JJ |
denotes |
inner |
| T1493 |
4877-4880 |
NN |
denotes |
ear |
| T1494 |
4892-4894 |
, |
denotes |
, |
| T1495 |
4894-4896 |
PRP |
denotes |
we |
| T1496 |
4897-4902 |
RB |
denotes |
first |
| T1497 |
4908-4910 |
TO |
denotes |
to |
| T1498 |
4911-4918 |
VB |
denotes |
present |
| T1499 |
4919-4922 |
DT |
denotes |
the |
| T1500 |
4923-4930 |
NNS |
denotes |
effects |
| T1501 |
4931-4933 |
IN |
denotes |
of |
| T1502 |
4934-4938 |
NN |
denotes |
BDNF |
| T1503 |
4953-4962 |
NNS |
denotes |
mutations |
| T1504 |
4939-4942 |
CC |
denotes |
and |
| T1505 |
4943-4945 |
NN |
denotes |
NT |
| T1506 |
4945-4946 |
HYPH |
denotes |
- |
| T1507 |
4946-4947 |
CD |
denotes |
3 |
| T1508 |
4948-4952 |
JJ |
denotes |
null |
| T1509 |
4963-4965 |
IN |
denotes |
at |
| T1510 |
4966-4971 |
NN |
denotes |
birth |
| T1511 |
4972-4973 |
-LRB- |
denotes |
[ |
| T1512 |
4978-4980 |
CD |
denotes |
14 |
| T1513 |
4973-4974 |
CD |
denotes |
6 |
| T1514 |
4974-4975 |
, |
denotes |
, |
| T1515 |
4975-4977 |
CD |
denotes |
13 |
| T1516 |
4977-4978 |
, |
denotes |
, |
| T1517 |
4980-4981 |
-RRB- |
denotes |
] |
| T1518 |
4981-4982 |
. |
denotes |
. |
| T1519 |
4982-5159 |
sentence |
denotes |
Null mutants of BDNF or its receptor trkB lose all innervation to the semicircular canals and have a reduced innervation to the utricle, saccule and apical turn of the cochlea. |
| T1520 |
4983-4987 |
JJ |
denotes |
Null |
| T1521 |
4988-4995 |
NNS |
denotes |
mutants |
| T1522 |
5025-5029 |
VBP |
denotes |
lose |
| T1523 |
4996-4998 |
IN |
denotes |
of |
| T1524 |
4999-5003 |
NN |
denotes |
BDNF |
| T1525 |
5004-5006 |
CC |
denotes |
or |
| T1526 |
5007-5010 |
PRP$ |
denotes |
its |
| T1527 |
5011-5019 |
NN |
denotes |
receptor |
| T1528 |
5020-5024 |
NN |
denotes |
trkB |
| T1529 |
5030-5033 |
DT |
denotes |
all |
| T1530 |
5034-5045 |
NN |
denotes |
innervation |
| T1531 |
5046-5048 |
IN |
denotes |
to |
| T1532 |
5049-5052 |
DT |
denotes |
the |
| T1533 |
5066-5072 |
NNS |
denotes |
canals |
| T1534 |
5053-5065 |
JJ |
denotes |
semicircular |
| T1535 |
5073-5076 |
CC |
denotes |
and |
| T1536 |
5077-5081 |
VBP |
denotes |
have |
| T1537 |
5082-5083 |
DT |
denotes |
a |
| T1538 |
5092-5103 |
NN |
denotes |
innervation |
| T1539 |
5084-5091 |
VBN |
denotes |
reduced |
| T1540 |
5104-5106 |
IN |
denotes |
to |
| T1541 |
5107-5110 |
DT |
denotes |
the |
| T1542 |
5111-5118 |
NN |
denotes |
utricle |
| T1543 |
5118-5120 |
, |
denotes |
, |
| T1544 |
5120-5127 |
NN |
denotes |
saccule |
| T1545 |
5128-5131 |
CC |
denotes |
and |
| T1546 |
5132-5138 |
JJ |
denotes |
apical |
| T1547 |
5139-5143 |
NN |
denotes |
turn |
| T1548 |
5144-5146 |
IN |
denotes |
of |
| T1549 |
5147-5150 |
DT |
denotes |
the |
| T1550 |
5151-5158 |
NN |
denotes |
cochlea |
| T1551 |
5158-5159 |
. |
denotes |
. |
| T1552 |
5159-5356 |
sentence |
denotes |
In contrast, null mutations of either NT-3 or its receptor trkC result in loss of spiral neurons in the basal turn with formation of an inner spiral bundle of afferents extending to the basal tip. |
| T1553 |
5160-5162 |
IN |
denotes |
In |
| T1554 |
5224-5230 |
VBP |
denotes |
result |
| T1555 |
5163-5171 |
NN |
denotes |
contrast |
| T1556 |
5171-5173 |
, |
denotes |
, |
| T1557 |
5173-5177 |
JJ |
denotes |
null |
| T1558 |
5178-5187 |
NNS |
denotes |
mutations |
| T1559 |
5188-5190 |
IN |
denotes |
of |
| T1560 |
5191-5197 |
CC |
denotes |
either |
| T1561 |
5198-5200 |
NN |
denotes |
NT |
| T1562 |
5200-5201 |
HYPH |
denotes |
- |
| T1563 |
5201-5202 |
CD |
denotes |
3 |
| T1564 |
5203-5205 |
CC |
denotes |
or |
| T1565 |
5206-5209 |
PRP$ |
denotes |
its |
| T1566 |
5210-5218 |
NN |
denotes |
receptor |
| T1567 |
5219-5223 |
NN |
denotes |
trkC |
| T1568 |
5231-5233 |
IN |
denotes |
in |
| T1569 |
5234-5238 |
NN |
denotes |
loss |
| T1570 |
5239-5241 |
IN |
denotes |
of |
| T1571 |
5242-5248 |
JJ |
denotes |
spiral |
| T1572 |
5249-5256 |
NNS |
denotes |
neurons |
| T1573 |
5257-5259 |
IN |
denotes |
in |
| T1574 |
5260-5263 |
DT |
denotes |
the |
| T1575 |
5270-5274 |
NN |
denotes |
turn |
| T1576 |
5264-5269 |
JJ |
denotes |
basal |
| T1577 |
5275-5279 |
IN |
denotes |
with |
| T1578 |
5280-5289 |
NN |
denotes |
formation |
| T1579 |
5290-5292 |
IN |
denotes |
of |
| T1580 |
5293-5295 |
DT |
denotes |
an |
| T1581 |
5309-5315 |
NN |
denotes |
bundle |
| T1582 |
5296-5301 |
JJ |
denotes |
inner |
| T1583 |
5302-5308 |
JJ |
denotes |
spiral |
| T1584 |
5316-5318 |
IN |
denotes |
of |
| T1585 |
5319-5328 |
NNS |
denotes |
afferents |
| T1586 |
5329-5338 |
VBG |
denotes |
extending |
| T1587 |
5339-5341 |
IN |
denotes |
to |
| T1588 |
5342-5345 |
DT |
denotes |
the |
| T1589 |
5352-5355 |
NN |
denotes |
tip |
| T1590 |
5346-5351 |
JJ |
denotes |
basal |
| T1591 |
5355-5356 |
. |
denotes |
. |
| T1592 |
5356-5547 |
sentence |
denotes |
Our null hypothesis for this study would be that Brn3c null mice show severe compromised production of these neurotrophins and should therefore show a comparable pattern of nerve fiber loss. |
| T1593 |
5357-5360 |
PRP$ |
denotes |
Our |
| T1594 |
5366-5376 |
NN |
denotes |
hypothesis |
| T1595 |
5361-5365 |
JJ |
denotes |
null |
| T1596 |
5398-5400 |
VB |
denotes |
be |
| T1597 |
5377-5380 |
IN |
denotes |
for |
| T1598 |
5381-5385 |
DT |
denotes |
this |
| T1599 |
5386-5391 |
NN |
denotes |
study |
| T1600 |
5392-5397 |
MD |
denotes |
would |
| T1601 |
5401-5405 |
IN |
denotes |
that |
| T1602 |
5422-5426 |
VBP |
denotes |
show |
| T1603 |
5406-5411 |
NN |
denotes |
Brn3c |
| T1604 |
5417-5421 |
NNS |
denotes |
mice |
| T1605 |
5412-5416 |
JJ |
denotes |
null |
| T1606 |
5427-5433 |
JJ |
denotes |
severe |
| T1607 |
5446-5456 |
NN |
denotes |
production |
| T1608 |
5434-5445 |
VBN |
denotes |
compromised |
| T1609 |
5457-5459 |
IN |
denotes |
of |
| T1610 |
5460-5465 |
DT |
denotes |
these |
| T1611 |
5466-5479 |
NNS |
denotes |
neurotrophins |
| T1612 |
5480-5483 |
CC |
denotes |
and |
| T1613 |
5484-5490 |
MD |
denotes |
should |
| T1614 |
5501-5505 |
VB |
denotes |
show |
| T1615 |
5491-5500 |
RB |
denotes |
therefore |
| T1616 |
5506-5507 |
DT |
denotes |
a |
| T1617 |
5519-5526 |
NN |
denotes |
pattern |
| T1618 |
5508-5518 |
JJ |
denotes |
comparable |
| T1619 |
5527-5529 |
IN |
denotes |
of |
| T1620 |
5530-5535 |
NN |
denotes |
nerve |
| T1621 |
5536-5541 |
NN |
denotes |
fiber |
| T1622 |
5542-5546 |
NN |
denotes |
loss |
| T1623 |
5546-5547 |
. |
denotes |
. |
| T1848 |
0-5567 |
NNS |
denotes |
mutant mice show long-term, incomplete retention of some afferent inner ear innervation
Abstract
Background
Ears of Brn3c null mutants develop immature hair cells, identifiable only by certain molecular markers, and undergo apoptosis in neonates. This partial development of hair cells could lead to enough neurotrophin expression to sustain sensory neurons through embryonic development. We have therefore investigated in these mutants the patterns of innervation and of expression of known neurotrophins.
Results
At birth there is a limited expression of BDNF and NT-3 in the mutant sensory epithelia and DiI tracing shows no specific reduction of afferents or efferents that resembles neurotrophin null mutations. At postnatal day 7/8 (P7/8), innervation is severely reduced both qualitatively and quantitatively. 1% of myosin VIIa-positive immature hair cells are present in the mutant cochlea, concentrated in the base. Around 20% of immature hair cells exist in the mutant vestibular sensory epithelia. Despite more severe loss of hair cells (1% compared to 20%), the cochlea retains many more sensory neurons (46% compared to 15%) than vestibular epithelia. Even 6 months old mutant mice have some fibers to all vestibular sensory epithelia and many more to the cochlear apex which lacks MyoVIIa positive hair cells. Topologically organized central cochlea projections exist at least until P8, suggesting that functional hair cells are not required to establish such projections.
Conclusion
The limited expression of neurotrophins in the cochlea of Brn3c null mice suffices to support many sensory neurons, particularly in the cochlea, until birth. The molecular nature of the long term survival of apical spiral neurons remains unclear.
Background
Brn3c is a POU domain factor that is crucial for inner ear hair cell development. Targeted null Brn3c mutants have no mature hair cells [1,2]. Close examination has revealed that some 'immature' hair cells form in Brn3c null mutants and express cellular markers such as Myosin VI and VIIa, calretinin and parvalbumin [3]. Furthermore, these immature hair cells of Brn3c null mutants undergo apoptosis in neonates [3]. Consistent with an apparent absence of mature hair cells, initial work suggested that all vestibular and most spiral ganglion cells are lost by postnatal day 14 (P14; [1]). However, more detailed quantification by others [3] reported that at P4 about 77% of vestibular neurons and only 29% of spiral neurons are lost. It was suggested that there is possibly a complete loss in adults [3]. Other than these preliminary statements, no data exists concerning the detailed pattern of loss of innervation in Brn3c null mutants.
The initial development and partial differentiation of hair cells in Brn3c mutants could possibly lead to some neurotrophin expression in these cells to sustain sensory neurons through embryonic development and beyond. Data on various single and compound neurotrophin null mutants have shown that the loss of a specific neurotrophin leads to topologically restricted loss of sensory neurons in the embryonic ear [4,5]. Such selective loss in Brn3c null mutants would therefore indicate reduction of a specific neurotrophin in immature hair cells. Moreover, recent work shows that in embryos NT-3 is primarily expressed in supporting cells, moving only around birth into hair cells [6,7]. In fact, the selective loss of vestibular as compared to cochlear sensory neurons (77% versus 29%; [3]) suggests that NT-3 expression may be less downregulated in Brn3c null mutants than BDNF [6,8,9], provided that at least some differentiation of supporting cells takes place. In the ear [4] as well as elsewhere [10] neurotrophins are progressively downregulated in postnatal mammals and possibly replaced by other factors [11].
We have investigated in detail the pattern of innervation in the Brn3c mutants, as well as the expression of NT-3 and BDNF. We want to evaluate a possible correlation between the topology of sensory neuron loss and absence of a specific neurotrophin or topological loss of hair cells at birth and in older animals. This information could be important for an in-depth evaluation of the human deafness related to the Pou4f3 gene, DFNA15 [12].
We report here long term retention of cochlear sensory neurons for at least 6 months, in particular in the cochlear apex, in Brn3c null mutant mice. This retention of afferents and efferents is unrelated to hair cell differentiation as not even immature hair cells can be detected at early postnatal stages with MyoVII immunocytochemistry in this part of the cochlea. This retention of apical spiral neurons is also largely unrelated to neurotrophins which are known to be reduced in their expression in neonatal rodents [4].
Results
To appreciate the effects of the Brn3c null mutation on the pattern of the inner ear innervation, we first want to present the effects of BDNF and NT-3 null mutations at birth [6,13,14]. Null mutants of BDNF or its receptor trkB lose all innervation to the semicircular canals and have a reduced innervation to the utricle, saccule and apical turn of the cochlea. In contrast, null mutations of either NT-3 or its receptor trkC result in loss of spiral neurons in the basal turn with formation of an inner spiral bundle of afferents extending to the basal tip. Our null hypothesis for this study would be that Brn3c null mice show severe compromised production of these neurotrophins and should therefore show a comparable pattern of nerve fiber loss.
Brn3c null mutants |
| R5 |
T191 |
T190 |
amod |
mutant,mice |
| R6 |
T193 |
T194 |
amod |
long,term |
| R8 |
T195 |
T194 |
punct |
-,term |
| R9 |
T196 |
T192 |
dobj |
retention,show |
| R10 |
T197 |
T196 |
punct |
", ",retention |
| R11 |
T198 |
T196 |
amod |
incomplete,retention |
| R12 |
T199 |
T196 |
prep |
of,retention |
| R13 |
T200 |
T201 |
det |
some,innervation |
| R14 |
T201 |
T199 |
pobj |
innervation,of |
| R15 |
T202 |
T201 |
amod |
afferent,innervation |
| R16 |
T203 |
T204 |
amod |
inner,ear |
| R17 |
T204 |
T201 |
compound |
ear,innervation |
| R18 |
T209 |
T210 |
nsubj |
Ears,develop |
| R19 |
T211 |
T209 |
prep |
of,Ears |
| R20 |
T212 |
T213 |
nmod |
Brn3c,mutants |
| R22 |
T214 |
T213 |
amod |
null,mutants |
| R23 |
T215 |
T216 |
amod |
immature,cells |
| R25 |
T217 |
T216 |
compound |
hair,cells |
| R26 |
T218 |
T216 |
punct |
", ",cells |
| R27 |
T219 |
T216 |
amod |
identifiable,cells |
| R28 |
T220 |
T221 |
advmod |
only,by |
| R29 |
T221 |
T219 |
prep |
by,identifiable |
| R30 |
T222 |
T223 |
amod |
certain,markers |
| R32 |
T224 |
T223 |
amod |
molecular,markers |
| R33 |
T225 |
T210 |
punct |
", ",develop |
| R34 |
T226 |
T210 |
cc |
and,develop |
| R35 |
T227 |
T210 |
conj |
undergo,develop |
| R36 |
T228 |
T227 |
dobj |
apoptosis,undergo |
| R37 |
T229 |
T227 |
prep |
in,undergo |
| R38 |
T230 |
T229 |
pobj |
neonates,in |
| R39 |
T231 |
T210 |
punct |
.,develop |
| R40 |
T233 |
T234 |
det |
This,development |
| R42 |
T235 |
T234 |
amod |
partial,development |
| R43 |
T237 |
T234 |
prep |
of,development |
| R44 |
T238 |
T239 |
compound |
hair,cells |
| R45 |
T239 |
T237 |
pobj |
cells,of |
| R46 |
T240 |
T236 |
aux |
could,lead |
| R47 |
T241 |
T236 |
prep |
to,lead |
| R48 |
T242 |
T243 |
amod |
enough,expression |
| R50 |
T244 |
T243 |
compound |
neurotrophin,expression |
| R51 |
T245 |
T246 |
aux |
to,sustain |
| R52 |
T246 |
T243 |
acl |
sustain,expression |
| R53 |
T247 |
T248 |
amod |
sensory,neurons |
| R54 |
T248 |
T246 |
dobj |
neurons,sustain |
| R55 |
T249 |
T246 |
prep |
through,sustain |
| R56 |
T250 |
T251 |
amod |
embryonic,development |
| R57 |
T251 |
T249 |
pobj |
development,through |
| R58 |
T252 |
T236 |
punct |
.,lead |
| R59 |
T254 |
T255 |
nsubj |
We,investigated |
| R60 |
T256 |
T255 |
aux |
have,investigated |
| R61 |
T257 |
T255 |
advmod |
therefore,investigated |
| R62 |
T258 |
T255 |
prep |
in,investigated |
| R63 |
T259 |
T260 |
det |
these,mutants |
| R64 |
T260 |
T258 |
pobj |
mutants,in |
| R65 |
T261 |
T262 |
det |
the,patterns |
| R66 |
T262 |
T255 |
dobj |
patterns,investigated |
| R67 |
T263 |
T262 |
prep |
of,patterns |
| R68 |
T264 |
T263 |
pobj |
innervation,of |
| R69 |
T265 |
T263 |
cc |
and,of |
| R70 |
T266 |
T263 |
conj |
of,of |
| R71 |
T267 |
T266 |
pobj |
expression,of |
| R72 |
T268 |
T266 |
prep |
of,of |
| R73 |
T269 |
T270 |
amod |
known,neurotrophins |
| R74 |
T270 |
T268 |
pobj |
neurotrophins,of |
| R75 |
T271 |
T255 |
punct |
.,investigated |
| R76 |
T275 |
T276 |
prep |
At,is |
| R77 |
T277 |
T275 |
pobj |
birth,At |
| R78 |
T278 |
T276 |
expl |
there,is |
| R79 |
T279 |
T280 |
det |
a,expression |
| R81 |
T281 |
T280 |
amod |
limited,expression |
| R82 |
T282 |
T280 |
prep |
of,expression |
| R83 |
T283 |
T282 |
pobj |
BDNF,of |
| R84 |
T284 |
T283 |
cc |
and,BDNF |
| R85 |
T285 |
T283 |
conj |
NT,BDNF |
| R86 |
T286 |
T285 |
punct |
-,NT |
| R87 |
T287 |
T285 |
nummod |
3,NT |
| R88 |
T288 |
T276 |
prep |
in,is |
| R89 |
T289 |
T290 |
det |
the,epithelia |
| R90 |
T290 |
T288 |
pobj |
epithelia,in |
| R91 |
T291 |
T290 |
amod |
mutant,epithelia |
| R92 |
T292 |
T290 |
amod |
sensory,epithelia |
| R93 |
T293 |
T276 |
cc |
and,is |
| R94 |
T294 |
T295 |
compound |
DiI,tracing |
| R95 |
T295 |
T296 |
nsubj |
tracing,shows |
| R96 |
T296 |
T276 |
conj |
shows,is |
| R97 |
T297 |
T298 |
det |
no,reduction |
| R99 |
T299 |
T298 |
amod |
specific,reduction |
| R100 |
T300 |
T298 |
prep |
of,reduction |
| R101 |
T301 |
T300 |
pobj |
afferents,of |
| R102 |
T302 |
T301 |
cc |
or,afferents |
| R103 |
T303 |
T301 |
conj |
efferents,afferents |
| R104 |
T304 |
T305 |
dep |
that,resembles |
| R105 |
T305 |
T301 |
relcl |
resembles,afferents |
| R106 |
T306 |
T307 |
nmod |
neurotrophin,mutations |
| R108 |
T308 |
T307 |
amod |
null,mutations |
| R109 |
T309 |
T276 |
punct |
.,is |
| R110 |
T311 |
T312 |
prep |
At,reduced |
| R111 |
T313 |
T314 |
amod |
postnatal,day |
| R112 |
T314 |
T311 |
pobj |
day,At |
| R113 |
T315 |
T314 |
nummod |
7,day |
| R114 |
T316 |
T314 |
punct |
/,day |
| R115 |
T317 |
T314 |
nummod |
8,day |
| R116 |
T318 |
T314 |
punct |
(,day |
| R117 |
T319 |
T314 |
appos |
P7,day |
| R118 |
T320 |
T319 |
punct |
/,P7 |
| R119 |
T321 |
T319 |
nummod |
8,P7 |
| R120 |
T322 |
T312 |
punct |
),reduced |
| R121 |
T323 |
T312 |
punct |
", ",reduced |
| R122 |
T324 |
T312 |
nsubjpass |
innervation,reduced |
| R123 |
T325 |
T312 |
auxpass |
is,reduced |
| R124 |
T326 |
T312 |
advmod |
severely,reduced |
| R125 |
T327 |
T328 |
preconj |
both,qualitatively |
| R126 |
T328 |
T312 |
advmod |
qualitatively,reduced |
| R127 |
T329 |
T328 |
cc |
and,qualitatively |
| R128 |
T330 |
T328 |
conj |
quantitatively,qualitatively |
| R129 |
T331 |
T312 |
punct |
.,reduced |
| R130 |
T333 |
T334 |
nummod |
1,% |
| R131 |
T334 |
T335 |
nsubj |
%,are |
| R132 |
T336 |
T334 |
prep |
of,% |
| R133 |
T337 |
T338 |
compound |
myosin,VIIa |
| R134 |
T338 |
T339 |
npadvmod |
VIIa,positive |
| R136 |
T340 |
T339 |
punct |
-,positive |
| R137 |
T341 |
T336 |
pobj |
cells,of |
| R138 |
T342 |
T341 |
amod |
immature,cells |
| R139 |
T343 |
T341 |
compound |
hair,cells |
| R140 |
T344 |
T335 |
acomp |
present,are |
| R141 |
T345 |
T335 |
prep |
in,are |
| R142 |
T346 |
T347 |
det |
the,cochlea |
| R144 |
T348 |
T347 |
amod |
mutant,cochlea |
| R145 |
T349 |
T335 |
punct |
", ",are |
| R146 |
T350 |
T335 |
advcl |
concentrated,are |
| R147 |
T351 |
T350 |
prep |
in,concentrated |
| R148 |
T352 |
T353 |
det |
the,base |
| R149 |
T353 |
T351 |
pobj |
base,in |
| R150 |
T354 |
T335 |
punct |
.,are |
| R151 |
T356 |
T357 |
quantmod |
Around,20 |
| R152 |
T357 |
T358 |
nummod |
20,% |
| R153 |
T358 |
T359 |
nsubj |
%,exist |
| R154 |
T360 |
T358 |
prep |
of,% |
| R155 |
T361 |
T362 |
amod |
immature,cells |
| R157 |
T363 |
T362 |
compound |
hair,cells |
| R158 |
T364 |
T359 |
prep |
in,exist |
| R159 |
T365 |
T366 |
det |
the,epithelia |
| R160 |
T366 |
T364 |
pobj |
epithelia,in |
| R161 |
T367 |
T366 |
amod |
mutant,epithelia |
| R162 |
T368 |
T366 |
amod |
vestibular,epithelia |
| R163 |
T369 |
T366 |
amod |
sensory,epithelia |
| R164 |
T370 |
T359 |
punct |
.,exist |
| R165 |
T372 |
T373 |
prep |
Despite,retains |
| R166 |
T374 |
T375 |
advmod |
more,severe |
| R167 |
T375 |
T376 |
amod |
severe,loss |
| R168 |
T376 |
T372 |
pobj |
loss,Despite |
| R169 |
T377 |
T376 |
prep |
of,loss |
| R170 |
T378 |
T379 |
compound |
hair,cells |
| R171 |
T379 |
T377 |
pobj |
cells,of |
| R172 |
T380 |
T381 |
punct |
(,% |
| R174 |
T382 |
T381 |
nummod |
1,% |
| R175 |
T383 |
T381 |
prep |
compared,% |
| R176 |
T384 |
T383 |
prep |
to,compared |
| R177 |
T385 |
T386 |
nummod |
20,% |
| R178 |
T386 |
T384 |
pobj |
%,to |
| R179 |
T387 |
T381 |
punct |
),% |
| R180 |
T388 |
T373 |
punct |
", ",retains |
| R181 |
T389 |
T390 |
det |
the,cochlea |
| R182 |
T390 |
T373 |
nsubj |
cochlea,retains |
| R183 |
T391 |
T392 |
amod |
many,neurons |
| R184 |
T392 |
T373 |
dobj |
neurons,retains |
| R185 |
T393 |
T392 |
amod |
more,neurons |
| R186 |
T394 |
T392 |
amod |
sensory,neurons |
| R187 |
T395 |
T396 |
punct |
(,% |
| R189 |
T397 |
T396 |
nummod |
46,% |
| R190 |
T398 |
T396 |
prep |
compared,% |
| R191 |
T399 |
T398 |
prep |
to,compared |
| R192 |
T400 |
T401 |
nummod |
15,% |
| R193 |
T401 |
T399 |
pobj |
%,to |
| R194 |
T402 |
T396 |
punct |
),% |
| R195 |
T403 |
T392 |
prep |
than,neurons |
| R196 |
T404 |
T405 |
amod |
vestibular,epithelia |
| R197 |
T405 |
T403 |
pobj |
epithelia,than |
| R198 |
T406 |
T373 |
punct |
.,retains |
| R199 |
T408 |
T409 |
advmod |
Even,mice |
| R200 |
T409 |
T414 |
nsubj |
mice,have |
| R201 |
T410 |
T411 |
nummod |
6,months |
| R202 |
T411 |
T412 |
npadvmod |
months,old |
| R203 |
T412 |
T409 |
amod |
old,mice |
| R204 |
T413 |
T409 |
amod |
mutant,mice |
| R205 |
T415 |
T416 |
det |
some,fibers |
| R206 |
T416 |
T414 |
dobj |
fibers,have |
| R207 |
T417 |
T416 |
prep |
to,fibers |
| R208 |
T418 |
T419 |
det |
all,epithelia |
| R209 |
T419 |
T417 |
pobj |
epithelia,to |
| R210 |
T420 |
T419 |
amod |
vestibular,epithelia |
| R211 |
T421 |
T419 |
amod |
sensory,epithelia |
| R212 |
T422 |
T416 |
cc |
and,fibers |
| R213 |
T423 |
T424 |
amod |
many,more |
| R214 |
T424 |
T416 |
conj |
more,fibers |
| R215 |
T425 |
T424 |
prep |
to,more |
| R216 |
T426 |
T427 |
det |
the,apex |
| R218 |
T428 |
T427 |
amod |
cochlear,apex |
| R219 |
T429 |
T430 |
dep |
which,lacks |
| R220 |
T430 |
T427 |
relcl |
lacks,apex |
| R221 |
T431 |
T432 |
npadvmod |
MyoVIIa,positive |
| R222 |
T432 |
T433 |
amod |
positive,cells |
| R224 |
T434 |
T433 |
compound |
hair,cells |
| R225 |
T435 |
T414 |
punct |
.,have |
| R226 |
T437 |
T438 |
advmod |
Topologically,organized |
| R227 |
T438 |
T439 |
amod |
organized,projections |
| R228 |
T439 |
T442 |
nsubj |
projections,exist |
| R229 |
T440 |
T441 |
amod |
central,cochlea |
| R230 |
T441 |
T439 |
compound |
cochlea,projections |
| R231 |
T443 |
T444 |
advmod |
at,least |
| R232 |
T444 |
T445 |
advmod |
least,until |
| R233 |
T445 |
T442 |
prep |
until,exist |
| R234 |
T446 |
T445 |
pobj |
P8,until |
| R235 |
T447 |
T442 |
punct |
", ",exist |
| R236 |
T448 |
T442 |
advcl |
suggesting,exist |
| R237 |
T449 |
T450 |
mark |
that,required |
| R238 |
T450 |
T448 |
ccomp |
required,suggesting |
| R239 |
T451 |
T452 |
amod |
functional,cells |
| R240 |
T452 |
T450 |
nsubjpass |
cells,required |
| R241 |
T453 |
T452 |
compound |
hair,cells |
| R242 |
T454 |
T450 |
auxpass |
are,required |
| R243 |
T455 |
T450 |
neg |
not,required |
| R244 |
T456 |
T457 |
aux |
to,establish |
| R245 |
T457 |
T450 |
advcl |
establish,required |
| R246 |
T458 |
T459 |
amod |
such,projections |
| R247 |
T459 |
T457 |
dobj |
projections,establish |
| R248 |
T460 |
T442 |
punct |
.,exist |
| R249 |
T464 |
T465 |
det |
The,expression |
| R251 |
T466 |
T465 |
amod |
limited,expression |
| R252 |
T468 |
T465 |
prep |
of,expression |
| R253 |
T469 |
T468 |
pobj |
neurotrophins,of |
| R254 |
T470 |
T465 |
prep |
in,expression |
| R255 |
T471 |
T472 |
det |
the,cochlea |
| R256 |
T472 |
T470 |
pobj |
cochlea,in |
| R257 |
T473 |
T472 |
prep |
of,cochlea |
| R258 |
T474 |
T475 |
npadvmod |
Brn3c,null |
| R259 |
T475 |
T476 |
amod |
null,mice |
| R260 |
T476 |
T473 |
pobj |
mice,of |
| R261 |
T477 |
T478 |
aux |
to,support |
| R262 |
T478 |
T467 |
xcomp |
support,suffices |
| R263 |
T479 |
T480 |
amod |
many,neurons |
| R265 |
T481 |
T480 |
amod |
sensory,neurons |
| R266 |
T482 |
T478 |
punct |
", ",support |
| R267 |
T483 |
T484 |
advmod |
particularly,in |
| R268 |
T484 |
T478 |
prep |
in,support |
| R269 |
T485 |
T486 |
det |
the,cochlea |
| R270 |
T486 |
T484 |
pobj |
cochlea,in |
| R271 |
T487 |
T478 |
punct |
", ",support |
| R272 |
T488 |
T478 |
prep |
until,support |
| R273 |
T489 |
T488 |
pobj |
birth,until |
| R274 |
T490 |
T467 |
punct |
.,suffices |
| R275 |
T492 |
T493 |
det |
The,nature |
| R277 |
T494 |
T493 |
amod |
molecular,nature |
| R278 |
T496 |
T493 |
prep |
of,nature |
| R279 |
T497 |
T498 |
det |
the,survival |
| R280 |
T498 |
T496 |
pobj |
survival,of |
| R281 |
T499 |
T500 |
amod |
long,term |
| R282 |
T500 |
T498 |
compound |
term,survival |
| R283 |
T501 |
T498 |
prep |
of,survival |
| R284 |
T502 |
T503 |
amod |
apical,spiral |
| R285 |
T503 |
T504 |
nmod |
spiral,neurons |
| R286 |
T504 |
T501 |
pobj |
neurons,of |
| R287 |
T505 |
T495 |
oprd |
unclear,remains |
| R288 |
T506 |
T495 |
punct |
.,remains |
| R300 |
T820 |
T821 |
nsubj |
Brn3c,is |
| R301 |
T822 |
T823 |
det |
a,factor |
| R302 |
T823 |
T821 |
attr |
factor,is |
| R303 |
T824 |
T825 |
compound |
POU,domain |
| R304 |
T825 |
T823 |
compound |
domain,factor |
| R305 |
T826 |
T827 |
dep |
that,is |
| R306 |
T827 |
T823 |
relcl |
is,factor |
| R307 |
T828 |
T827 |
acomp |
crucial,is |
| R308 |
T829 |
T828 |
prep |
for,crucial |
| R309 |
T830 |
T831 |
amod |
inner,ear |
| R310 |
T831 |
T832 |
nmod |
ear,development |
| R311 |
T832 |
T829 |
pobj |
development,for |
| R312 |
T833 |
T834 |
compound |
hair,cell |
| R313 |
T834 |
T832 |
compound |
cell,development |
| R314 |
T835 |
T821 |
punct |
.,is |
| R315 |
T837 |
T838 |
amod |
Targeted,mutants |
| R316 |
T838 |
T841 |
nsubj |
mutants,have |
| R317 |
T839 |
T838 |
amod |
null,mutants |
| R318 |
T840 |
T838 |
compound |
Brn3c,mutants |
| R319 |
T842 |
T843 |
det |
no,cells |
| R320 |
T843 |
T841 |
dobj |
cells,have |
| R321 |
T844 |
T843 |
amod |
mature,cells |
| R322 |
T845 |
T843 |
compound |
hair,cells |
| R323 |
T846 |
T847 |
punct |
[,2 |
| R324 |
T847 |
T841 |
parataxis |
2,have |
| R325 |
T848 |
T847 |
nummod |
1,2 |
| R326 |
T849 |
T847 |
punct |
",",2 |
| R327 |
T850 |
T847 |
punct |
],2 |
| R328 |
T851 |
T841 |
punct |
.,have |
| R329 |
T853 |
T854 |
compound |
Close,examination |
| R330 |
T854 |
T855 |
nsubj |
examination,revealed |
| R331 |
T856 |
T855 |
aux |
has,revealed |
| R332 |
T857 |
T858 |
mark |
that,form |
| R333 |
T858 |
T855 |
ccomp |
form,revealed |
| R334 |
T859 |
T860 |
det |
some,cells |
| R335 |
T860 |
T858 |
nsubj |
cells,form |
| R336 |
T861 |
T860 |
punct |
',cells |
| R337 |
T862 |
T860 |
amod |
immature,cells |
| R338 |
T863 |
T860 |
punct |
',cells |
| R339 |
T864 |
T860 |
compound |
hair,cells |
| R340 |
T865 |
T858 |
prep |
in,form |
| R341 |
T866 |
T867 |
nmod |
Brn3c,mutants |
| R342 |
T867 |
T865 |
pobj |
mutants,in |
| R343 |
T868 |
T867 |
amod |
null,mutants |
| R344 |
T869 |
T858 |
cc |
and,form |
| R345 |
T870 |
T858 |
conj |
express,form |
| R346 |
T871 |
T872 |
amod |
cellular,markers |
| R347 |
T872 |
T870 |
dobj |
markers,express |
| R348 |
T873 |
T874 |
amod |
such,as |
| R349 |
T874 |
T872 |
prep |
as,markers |
| R350 |
T875 |
T876 |
compound |
Myosin,VI |
| R351 |
T876 |
T874 |
pobj |
VI,as |
| R352 |
T877 |
T876 |
cc |
and,VI |
| R353 |
T878 |
T876 |
conj |
VIIa,VI |
| R354 |
T879 |
T876 |
punct |
", ",VI |
| R355 |
T880 |
T876 |
conj |
calretinin,VI |
| R356 |
T881 |
T880 |
cc |
and,calretinin |
| R357 |
T882 |
T880 |
conj |
parvalbumin,calretinin |
| R358 |
T883 |
T884 |
punct |
[,3 |
| R359 |
T884 |
T855 |
parataxis |
3,revealed |
| R360 |
T885 |
T884 |
punct |
],3 |
| R361 |
T886 |
T855 |
punct |
.,revealed |
| R362 |
T888 |
T889 |
advmod |
Furthermore,undergo |
| R363 |
T890 |
T889 |
punct |
", ",undergo |
| R364 |
T891 |
T892 |
det |
these,cells |
| R365 |
T892 |
T889 |
nsubj |
cells,undergo |
| R366 |
T893 |
T892 |
amod |
immature,cells |
| R367 |
T894 |
T892 |
compound |
hair,cells |
| R368 |
T895 |
T892 |
prep |
of,cells |
| R369 |
T896 |
T897 |
nmod |
Brn3c,mutants |
| R370 |
T897 |
T895 |
pobj |
mutants,of |
| R371 |
T898 |
T897 |
amod |
null,mutants |
| R372 |
T899 |
T889 |
dobj |
apoptosis,undergo |
| R373 |
T900 |
T889 |
prep |
in,undergo |
| R374 |
T901 |
T900 |
pobj |
neonates,in |
| R375 |
T902 |
T903 |
punct |
[,3 |
| R376 |
T903 |
T889 |
parataxis |
3,undergo |
| R377 |
T904 |
T903 |
punct |
],3 |
| R378 |
T905 |
T889 |
punct |
.,undergo |
| R379 |
T907 |
T908 |
advcl |
Consistent,suggested |
| R380 |
T909 |
T907 |
prep |
with,Consistent |
| R381 |
T910 |
T911 |
det |
an,absence |
| R382 |
T911 |
T909 |
pobj |
absence,with |
| R383 |
T912 |
T911 |
amod |
apparent,absence |
| R384 |
T913 |
T911 |
prep |
of,absence |
| R385 |
T914 |
T915 |
amod |
mature,cells |
| R386 |
T915 |
T913 |
pobj |
cells,of |
| R387 |
T916 |
T915 |
compound |
hair,cells |
| R388 |
T917 |
T908 |
punct |
", ",suggested |
| R389 |
T918 |
T919 |
amod |
initial,work |
| R390 |
T919 |
T908 |
nsubj |
work,suggested |
| R391 |
T920 |
T921 |
mark |
that,lost |
| R392 |
T921 |
T908 |
ccomp |
lost,suggested |
| R393 |
T922 |
T923 |
det |
all,vestibular |
| R394 |
T923 |
T921 |
nsubjpass |
vestibular,lost |
| R395 |
T924 |
T923 |
cc |
and,vestibular |
| R396 |
T925 |
T926 |
det |
most,spiral |
| R397 |
T926 |
T927 |
nmod |
spiral,cells |
| R398 |
T927 |
T923 |
conj |
cells,vestibular |
| R399 |
T928 |
T927 |
compound |
ganglion,cells |
| R400 |
T929 |
T921 |
auxpass |
are,lost |
| R401 |
T930 |
T921 |
prep |
by,lost |
| R402 |
T931 |
T932 |
amod |
postnatal,day |
| R403 |
T932 |
T930 |
pobj |
day,by |
| R404 |
T933 |
T932 |
nummod |
14,day |
| R405 |
T934 |
T932 |
punct |
(,day |
| R406 |
T935 |
T932 |
appos |
P14,day |
| R407 |
T936 |
T921 |
punct |
;,lost |
| R408 |
T937 |
T938 |
punct |
[,1 |
| R409 |
T938 |
T921 |
parataxis |
1,lost |
| R410 |
T939 |
T938 |
punct |
],1 |
| R411 |
T940 |
T921 |
punct |
),lost |
| R412 |
T941 |
T908 |
punct |
.,suggested |
| R413 |
T943 |
T944 |
advmod |
However,reported |
| R414 |
T945 |
T944 |
punct |
", ",reported |
| R415 |
T946 |
T947 |
advmod |
more,quantification |
| R416 |
T947 |
T944 |
nsubj |
quantification,reported |
| R417 |
T948 |
T947 |
amod |
detailed,quantification |
| R418 |
T949 |
T947 |
prep |
by,quantification |
| R419 |
T950 |
T949 |
pobj |
others,by |
| R420 |
T951 |
T952 |
punct |
[,3 |
| R421 |
T952 |
T947 |
parataxis |
3,quantification |
| R422 |
T953 |
T952 |
punct |
],3 |
| R423 |
T954 |
T955 |
mark |
that,lost |
| R424 |
T955 |
T944 |
ccomp |
lost,reported |
| R425 |
T956 |
T955 |
prep |
at,lost |
| R426 |
T957 |
T956 |
pobj |
P4,at |
| R427 |
T958 |
T959 |
quantmod |
about,77 |
| R428 |
T959 |
T960 |
nummod |
77,% |
| R429 |
T960 |
T955 |
nsubjpass |
%,lost |
| R430 |
T961 |
T960 |
prep |
of,% |
| R431 |
T962 |
T963 |
amod |
vestibular,neurons |
| R432 |
T963 |
T961 |
pobj |
neurons,of |
| R433 |
T964 |
T960 |
cc |
and,% |
| R434 |
T965 |
T966 |
advmod |
only,29 |
| R435 |
T966 |
T967 |
nummod |
29,% |
| R436 |
T967 |
T960 |
conj |
%,% |
| R437 |
T968 |
T967 |
prep |
of,% |
| R438 |
T969 |
T970 |
amod |
spiral,neurons |
| R439 |
T970 |
T968 |
pobj |
neurons,of |
| R440 |
T971 |
T955 |
auxpass |
are,lost |
| R441 |
T972 |
T944 |
punct |
.,reported |
| R442 |
T974 |
T975 |
nsubjpass |
It,suggested |
| R443 |
T976 |
T975 |
auxpass |
was,suggested |
| R444 |
T977 |
T978 |
mark |
that,is |
| R445 |
T978 |
T975 |
ccomp |
is,suggested |
| R446 |
T979 |
T978 |
expl |
there,is |
| R447 |
T980 |
T978 |
advmod |
possibly,is |
| R448 |
T981 |
T982 |
det |
a,loss |
| R449 |
T982 |
T978 |
attr |
loss,is |
| R450 |
T983 |
T982 |
amod |
complete,loss |
| R451 |
T984 |
T978 |
prep |
in,is |
| R452 |
T985 |
T984 |
pobj |
adults,in |
| R453 |
T986 |
T987 |
punct |
[,3 |
| R454 |
T987 |
T975 |
parataxis |
3,suggested |
| R455 |
T988 |
T987 |
punct |
],3 |
| R456 |
T989 |
T975 |
punct |
.,suggested |
| R457 |
T991 |
T992 |
advmod |
Other,exists |
| R458 |
T993 |
T991 |
prep |
than,Other |
| R459 |
T994 |
T995 |
det |
these,statements |
| R460 |
T995 |
T993 |
pobj |
statements,than |
| R461 |
T996 |
T995 |
amod |
preliminary,statements |
| R462 |
T997 |
T992 |
punct |
", ",exists |
| R463 |
T998 |
T999 |
det |
no,data |
| R464 |
T999 |
T992 |
nsubj |
data,exists |
| R465 |
T1000 |
T992 |
prep |
concerning,exists |
| R466 |
T1001 |
T1002 |
det |
the,pattern |
| R467 |
T1002 |
T1000 |
pobj |
pattern,concerning |
| R468 |
T1003 |
T1002 |
amod |
detailed,pattern |
| R469 |
T1004 |
T1002 |
prep |
of,pattern |
| R470 |
T1005 |
T1004 |
pobj |
loss,of |
| R471 |
T1006 |
T1005 |
prep |
of,loss |
| R472 |
T1007 |
T1006 |
pobj |
innervation,of |
| R473 |
T1008 |
T1005 |
prep |
in,loss |
| R474 |
T1009 |
T1010 |
npadvmod |
Brn3c,null |
| R475 |
T1010 |
T1011 |
amod |
null,mutants |
| R476 |
T1011 |
T1008 |
pobj |
mutants,in |
| R477 |
T1012 |
T992 |
punct |
.,exists |
| R478 |
T1014 |
T1015 |
det |
The,development |
| R479 |
T1015 |
T1017 |
nsubj |
development,lead |
| R480 |
T1016 |
T1015 |
amod |
initial,development |
| R481 |
T1018 |
T1015 |
cc |
and,development |
| R482 |
T1019 |
T1020 |
amod |
partial,differentiation |
| R483 |
T1020 |
T1015 |
conj |
differentiation,development |
| R484 |
T1021 |
T1015 |
prep |
of,development |
| R485 |
T1022 |
T1023 |
compound |
hair,cells |
| R486 |
T1023 |
T1021 |
pobj |
cells,of |
| R487 |
T1024 |
T1015 |
prep |
in,development |
| R488 |
T1025 |
T1026 |
compound |
Brn3c,mutants |
| R489 |
T1026 |
T1024 |
pobj |
mutants,in |
| R490 |
T1027 |
T1017 |
aux |
could,lead |
| R491 |
T1028 |
T1017 |
advmod |
possibly,lead |
| R492 |
T1029 |
T1017 |
prep |
to,lead |
| R493 |
T1030 |
T1031 |
det |
some,expression |
| R494 |
T1031 |
T1029 |
pobj |
expression,to |
| R495 |
T1032 |
T1031 |
compound |
neurotrophin,expression |
| R496 |
T1033 |
T1031 |
prep |
in,expression |
| R497 |
T1034 |
T1035 |
det |
these,cells |
| R498 |
T1035 |
T1033 |
pobj |
cells,in |
| R499 |
T1036 |
T1037 |
aux |
to,sustain |
| R500 |
T1037 |
T1017 |
advcl |
sustain,lead |
| R501 |
T1038 |
T1039 |
amod |
sensory,neurons |
| R502 |
T1039 |
T1037 |
dobj |
neurons,sustain |
| R503 |
T1040 |
T1037 |
prep |
through,sustain |
| R504 |
T1041 |
T1042 |
amod |
embryonic,development |
| R505 |
T1042 |
T1040 |
pobj |
development,through |
| R506 |
T1043 |
T1040 |
cc |
and,through |
| R507 |
T1044 |
T1040 |
conj |
beyond,through |
| R508 |
T1045 |
T1017 |
punct |
.,lead |
| R509 |
T1047 |
T1048 |
nsubj |
Data,shown |
| R510 |
T1049 |
T1047 |
prep |
on,Data |
| R511 |
T1050 |
T1051 |
amod |
various,mutants |
| R512 |
T1051 |
T1049 |
pobj |
mutants,on |
| R513 |
T1052 |
T1053 |
amod |
single,neurotrophin |
| R514 |
T1053 |
T1056 |
npadvmod |
neurotrophin,null |
| R515 |
T1054 |
T1052 |
cc |
and,single |
| R516 |
T1055 |
T1052 |
conj |
compound,single |
| R517 |
T1056 |
T1051 |
amod |
null,mutants |
| R518 |
T1057 |
T1048 |
aux |
have,shown |
| R519 |
T1058 |
T1059 |
mark |
that,leads |
| R520 |
T1059 |
T1048 |
ccomp |
leads,shown |
| R521 |
T1060 |
T1061 |
det |
the,loss |
| R522 |
T1061 |
T1059 |
nsubj |
loss,leads |
| R523 |
T1062 |
T1061 |
prep |
of,loss |
| R524 |
T1063 |
T1064 |
det |
a,neurotrophin |
| R525 |
T1064 |
T1062 |
pobj |
neurotrophin,of |
| R526 |
T1065 |
T1064 |
amod |
specific,neurotrophin |
| R527 |
T1066 |
T1059 |
prep |
to,leads |
| R528 |
T1067 |
T1068 |
advmod |
topologically,restricted |
| R529 |
T1068 |
T1069 |
amod |
restricted,loss |
| R530 |
T1069 |
T1066 |
pobj |
loss,to |
| R531 |
T1070 |
T1069 |
prep |
of,loss |
| R532 |
T1071 |
T1072 |
amod |
sensory,neurons |
| R533 |
T1072 |
T1070 |
pobj |
neurons,of |
| R534 |
T1073 |
T1069 |
prep |
in,loss |
| R535 |
T1074 |
T1075 |
det |
the,ear |
| R536 |
T1075 |
T1073 |
pobj |
ear,in |
| R537 |
T1076 |
T1075 |
amod |
embryonic,ear |
| R538 |
T1077 |
T1078 |
punct |
[,5 |
| R539 |
T1078 |
T1048 |
parataxis |
5,shown |
| R540 |
T1079 |
T1078 |
nummod |
4,5 |
| R541 |
T1080 |
T1078 |
punct |
",",5 |
| R542 |
T1081 |
T1078 |
punct |
],5 |
| R543 |
T1082 |
T1048 |
punct |
.,shown |
| R544 |
T1084 |
T1085 |
amod |
Such,loss |
| R545 |
T1085 |
T1087 |
nsubj |
loss,indicate |
| R546 |
T1086 |
T1085 |
amod |
selective,loss |
| R547 |
T1088 |
T1085 |
prep |
in,loss |
| R548 |
T1089 |
T1090 |
nmod |
Brn3c,mutants |
| R549 |
T1090 |
T1088 |
pobj |
mutants,in |
| R550 |
T1091 |
T1090 |
amod |
null,mutants |
| R551 |
T1092 |
T1087 |
aux |
would,indicate |
| R552 |
T1093 |
T1087 |
advmod |
therefore,indicate |
| R553 |
T1094 |
T1087 |
dobj |
reduction,indicate |
| R554 |
T1095 |
T1094 |
prep |
of,reduction |
| R555 |
T1096 |
T1097 |
det |
a,neurotrophin |
| R556 |
T1097 |
T1095 |
pobj |
neurotrophin,of |
| R557 |
T1098 |
T1097 |
amod |
specific,neurotrophin |
| R558 |
T1099 |
T1094 |
prep |
in,reduction |
| R559 |
T1100 |
T1101 |
amod |
immature,cells |
| R560 |
T1101 |
T1099 |
pobj |
cells,in |
| R561 |
T1102 |
T1101 |
compound |
hair,cells |
| R562 |
T1103 |
T1087 |
punct |
.,indicate |
| R563 |
T1105 |
T1106 |
advmod |
Moreover,shows |
| R564 |
T1107 |
T1106 |
punct |
", ",shows |
| R565 |
T1108 |
T1109 |
amod |
recent,work |
| R566 |
T1109 |
T1106 |
nsubj |
work,shows |
| R567 |
T1110 |
T1111 |
mark |
that,expressed |
| R568 |
T1111 |
T1106 |
ccomp |
expressed,shows |
| R569 |
T1112 |
T1111 |
prep |
in,expressed |
| R570 |
T1113 |
T1112 |
pobj |
embryos,in |
| R571 |
T1114 |
T1111 |
nsubjpass |
NT,expressed |
| R572 |
T1115 |
T1114 |
punct |
-,NT |
| R573 |
T1116 |
T1114 |
nummod |
3,NT |
| R574 |
T1117 |
T1111 |
auxpass |
is,expressed |
| R575 |
T1118 |
T1111 |
advmod |
primarily,expressed |
| R576 |
T1119 |
T1111 |
prep |
in,expressed |
| R577 |
T1120 |
T1121 |
amod |
supporting,cells |
| R578 |
T1121 |
T1119 |
pobj |
cells,in |
| R579 |
T1122 |
T1111 |
punct |
", ",expressed |
| R580 |
T1123 |
T1111 |
advcl |
moving,expressed |
| R581 |
T1124 |
T1125 |
advmod |
only,around |
| R582 |
T1125 |
T1123 |
prep |
around,moving |
| R583 |
T1126 |
T1125 |
pobj |
birth,around |
| R584 |
T1127 |
T1123 |
prep |
into,moving |
| R585 |
T1128 |
T1129 |
compound |
hair,cells |
| R586 |
T1129 |
T1127 |
pobj |
cells,into |
| R587 |
T1130 |
T1131 |
punct |
[,7 |
| R588 |
T1131 |
T1106 |
parataxis |
7,shows |
| R589 |
T1132 |
T1131 |
nummod |
6,7 |
| R590 |
T1133 |
T1131 |
punct |
",",7 |
| R591 |
T1134 |
T1131 |
punct |
],7 |
| R592 |
T1135 |
T1106 |
punct |
.,shows |
| R593 |
T1137 |
T1138 |
prep |
In,suggests |
| R594 |
T1139 |
T1137 |
pobj |
fact,In |
| R595 |
T1140 |
T1138 |
punct |
", ",suggests |
| R596 |
T1141 |
T1142 |
det |
the,loss |
| R597 |
T1142 |
T1138 |
nsubj |
loss,suggests |
| R598 |
T1143 |
T1142 |
amod |
selective,loss |
| R599 |
T1144 |
T1142 |
prep |
of,loss |
| R600 |
T1145 |
T1144 |
pobj |
vestibular,of |
| R601 |
T1146 |
T1145 |
prep |
as,vestibular |
| R602 |
T1147 |
T1146 |
prep |
compared,as |
| R603 |
T1148 |
T1147 |
prep |
to,compared |
| R604 |
T1149 |
T1148 |
pobj |
cochlear,to |
| R605 |
T1150 |
T1151 |
amod |
sensory,neurons |
| R606 |
T1151 |
T1145 |
appos |
neurons,vestibular |
| R607 |
T1152 |
T1153 |
punct |
(,% |
| R608 |
T1153 |
T1145 |
parataxis |
%,vestibular |
| R609 |
T1154 |
T1153 |
nummod |
77,% |
| R610 |
T1155 |
T1153 |
cc |
versus,% |
| R611 |
T1156 |
T1157 |
nummod |
29,% |
| R612 |
T1157 |
T1153 |
conj |
%,% |
| R613 |
T1158 |
T1153 |
punct |
;,% |
| R614 |
T1159 |
T1160 |
punct |
[,3 |
| R615 |
T1160 |
T1153 |
parataxis |
3,% |
| R616 |
T1161 |
T1160 |
punct |
],3 |
| R617 |
T1162 |
T1153 |
punct |
),% |
| R618 |
T1163 |
T1164 |
mark |
that,be |
| R619 |
T1164 |
T1138 |
ccomp |
be,suggests |
| R620 |
T1165 |
T1166 |
nmod |
NT,expression |
| R621 |
T1166 |
T1164 |
nsubj |
expression,be |
| R622 |
T1167 |
T1165 |
punct |
-,NT |
| R623 |
T1168 |
T1165 |
nummod |
3,NT |
| R624 |
T1169 |
T1164 |
aux |
may,be |
| R625 |
T1170 |
T1171 |
advmod |
less,downregulated |
| R626 |
T1171 |
T1164 |
acomp |
downregulated,be |
| R627 |
T1172 |
T1164 |
prep |
in,be |
| R628 |
T1173 |
T1174 |
nmod |
Brn3c,mutants |
| R629 |
T1174 |
T1172 |
pobj |
mutants,in |
| R630 |
T1175 |
T1174 |
amod |
null,mutants |
| R631 |
T1176 |
T1164 |
prep |
than,be |
| R632 |
T1177 |
T1176 |
pobj |
BDNF,than |
| R633 |
T1178 |
T1179 |
punct |
[,9 |
| R634 |
T1179 |
T1164 |
parataxis |
9,be |
| R635 |
T1180 |
T1179 |
nummod |
6,9 |
| R636 |
T1181 |
T1179 |
punct |
",",9 |
| R637 |
T1182 |
T1179 |
nummod |
8,9 |
| R638 |
T1183 |
T1179 |
punct |
",",9 |
| R639 |
T1184 |
T1179 |
punct |
],9 |
| R640 |
T1185 |
T1164 |
punct |
", ",be |
| R641 |
T1186 |
T1164 |
prep |
provided,be |
| R642 |
T1187 |
T1188 |
mark |
that,takes |
| R643 |
T1188 |
T1186 |
pcomp |
takes,provided |
| R644 |
T1189 |
T1190 |
advmod |
at,least |
| R645 |
T1190 |
T1191 |
advmod |
least,differentiation |
| R646 |
T1191 |
T1188 |
nsubj |
differentiation,takes |
| R647 |
T1192 |
T1191 |
det |
some,differentiation |
| R648 |
T1193 |
T1191 |
prep |
of,differentiation |
| R649 |
T1194 |
T1195 |
amod |
supporting,cells |
| R650 |
T1195 |
T1193 |
pobj |
cells,of |
| R651 |
T1196 |
T1188 |
dobj |
place,takes |
| R652 |
T1197 |
T1138 |
punct |
.,suggests |
| R653 |
T1199 |
T1200 |
prep |
In,downregulated |
| R654 |
T1201 |
T1202 |
det |
the,ear |
| R655 |
T1202 |
T1199 |
pobj |
ear,In |
| R656 |
T1203 |
T1204 |
punct |
[,4 |
| R657 |
T1204 |
T1199 |
parataxis |
4,In |
| R658 |
T1205 |
T1204 |
punct |
],4 |
| R659 |
T1206 |
T1207 |
advmod |
as,as |
| R660 |
T1207 |
T1199 |
cc |
as,In |
| R661 |
T1208 |
T1207 |
advmod |
well,as |
| R662 |
T1209 |
T1210 |
advmod |
elsewhere,10 |
| R663 |
T1210 |
T1199 |
conj |
10,In |
| R664 |
T1211 |
T1210 |
punct |
[,10 |
| R665 |
T1212 |
T1210 |
punct |
],10 |
| R666 |
T1213 |
T1200 |
nsubjpass |
neurotrophins,downregulated |
| R667 |
T1214 |
T1200 |
auxpass |
are,downregulated |
| R668 |
T1215 |
T1200 |
advmod |
progressively,downregulated |
| R669 |
T1216 |
T1200 |
prep |
in,downregulated |
| R670 |
T1217 |
T1218 |
amod |
postnatal,mammals |
| R671 |
T1218 |
T1216 |
pobj |
mammals,in |
| R672 |
T1219 |
T1200 |
cc |
and,downregulated |
| R673 |
T1220 |
T1221 |
advmod |
possibly,replaced |
| R674 |
T1221 |
T1200 |
conj |
replaced,downregulated |
| R675 |
T1222 |
T1221 |
agent |
by,replaced |
| R676 |
T1223 |
T1224 |
amod |
other,factors |
| R677 |
T1224 |
T1222 |
pobj |
factors,by |
| R678 |
T1225 |
T1226 |
punct |
[,11 |
| R679 |
T1226 |
T1221 |
parataxis |
11,replaced |
| R680 |
T1227 |
T1226 |
punct |
],11 |
| R681 |
T1228 |
T1200 |
punct |
.,downregulated |
| R682 |
T1230 |
T1231 |
nsubj |
We,investigated |
| R683 |
T1232 |
T1231 |
aux |
have,investigated |
| R684 |
T1233 |
T1231 |
prep |
in,investigated |
| R685 |
T1234 |
T1233 |
pobj |
detail,in |
| R686 |
T1235 |
T1236 |
det |
the,pattern |
| R687 |
T1236 |
T1231 |
dobj |
pattern,investigated |
| R688 |
T1237 |
T1236 |
prep |
of,pattern |
| R689 |
T1238 |
T1237 |
pobj |
innervation,of |
| R690 |
T1239 |
T1236 |
prep |
in,pattern |
| R691 |
T1240 |
T1241 |
det |
the,mutants |
| R692 |
T1241 |
T1239 |
pobj |
mutants,in |
| R693 |
T1242 |
T1241 |
compound |
Brn3c,mutants |
| R694 |
T1243 |
T1236 |
punct |
", ",pattern |
| R695 |
T1244 |
T1245 |
advmod |
as,as |
| R696 |
T1245 |
T1236 |
cc |
as,pattern |
| R697 |
T1246 |
T1245 |
advmod |
well,as |
| R698 |
T1247 |
T1248 |
det |
the,expression |
| R699 |
T1248 |
T1236 |
conj |
expression,pattern |
| R700 |
T1249 |
T1248 |
prep |
of,expression |
| R701 |
T1250 |
T1249 |
pobj |
NT,of |
| R702 |
T1251 |
T1250 |
punct |
-,NT |
| R703 |
T1252 |
T1250 |
nummod |
3,NT |
| R704 |
T1253 |
T1250 |
cc |
and,NT |
| R705 |
T1254 |
T1250 |
conj |
BDNF,NT |
| R706 |
T1255 |
T1231 |
punct |
.,investigated |
| R707 |
T1257 |
T1258 |
nsubj |
We,want |
| R708 |
T1259 |
T1260 |
aux |
to,evaluate |
| R709 |
T1260 |
T1258 |
xcomp |
evaluate,want |
| R710 |
T1261 |
T1262 |
det |
a,correlation |
| R711 |
T1262 |
T1260 |
dobj |
correlation,evaluate |
| R712 |
T1263 |
T1262 |
amod |
possible,correlation |
| R713 |
T1264 |
T1262 |
prep |
between,correlation |
| R714 |
T1265 |
T1266 |
det |
the,topology |
| R715 |
T1266 |
T1264 |
pobj |
topology,between |
| R716 |
T1267 |
T1266 |
prep |
of,topology |
| R717 |
T1268 |
T1269 |
amod |
sensory,neuron |
| R718 |
T1269 |
T1270 |
compound |
neuron,loss |
| R719 |
T1270 |
T1267 |
pobj |
loss,of |
| R720 |
T1271 |
T1266 |
cc |
and,topology |
| R721 |
T1272 |
T1266 |
conj |
absence,topology |
| R722 |
T1273 |
T1272 |
prep |
of,absence |
| R723 |
T1274 |
T1275 |
det |
a,neurotrophin |
| R724 |
T1275 |
T1273 |
pobj |
neurotrophin,of |
| R725 |
T1276 |
T1275 |
amod |
specific,neurotrophin |
| R726 |
T1277 |
T1275 |
cc |
or,neurotrophin |
| R727 |
T1278 |
T1279 |
amod |
topological,loss |
| R728 |
T1279 |
T1275 |
conj |
loss,neurotrophin |
| R729 |
T1280 |
T1279 |
prep |
of,loss |
| R730 |
T1281 |
T1282 |
compound |
hair,cells |
| R731 |
T1282 |
T1280 |
pobj |
cells,of |
| R732 |
T1283 |
T1262 |
prep |
at,correlation |
| R733 |
T1284 |
T1283 |
pobj |
birth,at |
| R734 |
T1285 |
T1283 |
cc |
and,at |
| R735 |
T1286 |
T1283 |
conj |
in,at |
| R736 |
T1287 |
T1288 |
amod |
older,animals |
| R737 |
T1288 |
T1286 |
pobj |
animals,in |
| R738 |
T1289 |
T1258 |
punct |
.,want |
| R739 |
T1291 |
T1292 |
det |
This,information |
| R740 |
T1292 |
T1293 |
nsubj |
information,be |
| R741 |
T1294 |
T1293 |
aux |
could,be |
| R742 |
T1295 |
T1293 |
acomp |
important,be |
| R743 |
T1296 |
T1295 |
prep |
for,important |
| R744 |
T1297 |
T1298 |
det |
an,evaluation |
| R745 |
T1298 |
T1296 |
pobj |
evaluation,for |
| R746 |
T1299 |
T1298 |
nmod |
in,evaluation |
| R747 |
T1300 |
T1299 |
punct |
-,in |
| R748 |
T1301 |
T1299 |
pobj |
depth,in |
| R749 |
T1302 |
T1298 |
prep |
of,evaluation |
| R750 |
T1303 |
T1304 |
det |
the,deafness |
| R751 |
T1304 |
T1302 |
pobj |
deafness,of |
| R752 |
T1305 |
T1304 |
amod |
human,deafness |
| R753 |
T1306 |
T1304 |
acl |
related,deafness |
| R754 |
T1307 |
T1306 |
prep |
to,related |
| R755 |
T1308 |
T1309 |
det |
the,gene |
| R756 |
T1309 |
T1307 |
pobj |
gene,to |
| R757 |
T1310 |
T1309 |
compound |
Pou4f3,gene |
| R758 |
T1311 |
T1309 |
punct |
", ",gene |
| R759 |
T1312 |
T1309 |
appos |
DFNA15,gene |
| R760 |
T1313 |
T1314 |
punct |
[,12 |
| R761 |
T1314 |
T1293 |
parataxis |
12,be |
| R762 |
T1315 |
T1314 |
punct |
],12 |
| R763 |
T1316 |
T1293 |
punct |
.,be |
| R764 |
T1318 |
T1319 |
nsubj |
We,report |
| R765 |
T1320 |
T1319 |
advmod |
here,report |
| R766 |
T1321 |
T1322 |
amod |
long,term |
| R767 |
T1322 |
T1323 |
compound |
term,retention |
| R768 |
T1323 |
T1319 |
dobj |
retention,report |
| R769 |
T1324 |
T1323 |
prep |
of,retention |
| R770 |
T1325 |
T1326 |
amod |
cochlear,neurons |
| R771 |
T1326 |
T1324 |
pobj |
neurons,of |
| R772 |
T1327 |
T1326 |
amod |
sensory,neurons |
| R773 |
T1328 |
T1323 |
prep |
for,retention |
| R774 |
T1329 |
T1330 |
advmod |
at,6 |
| R775 |
T1330 |
T1332 |
nummod |
6,months |
| R776 |
T1331 |
T1330 |
advmod |
least,6 |
| R777 |
T1332 |
T1328 |
pobj |
months,for |
| R778 |
T1333 |
T1323 |
punct |
", ",retention |
| R779 |
T1334 |
T1335 |
prep |
in,in |
| R780 |
T1335 |
T1323 |
prep |
in,retention |
| R781 |
T1336 |
T1334 |
amod |
particular,in |
| R782 |
T1337 |
T1338 |
det |
the,apex |
| R783 |
T1338 |
T1335 |
pobj |
apex,in |
| R784 |
T1339 |
T1338 |
amod |
cochlear,apex |
| R785 |
T1340 |
T1323 |
punct |
", ",retention |
| R786 |
T1341 |
T1323 |
prep |
in,retention |
| R787 |
T1342 |
T1343 |
npadvmod |
Brn3c,null |
| R788 |
T1343 |
T1344 |
amod |
null,mice |
| R789 |
T1344 |
T1341 |
pobj |
mice,in |
| R790 |
T1345 |
T1344 |
amod |
mutant,mice |
| R791 |
T1346 |
T1319 |
punct |
.,report |
| R792 |
T1348 |
T1349 |
det |
This,retention |
| R793 |
T1349 |
T1350 |
nsubj |
retention,is |
| R794 |
T1351 |
T1349 |
prep |
of,retention |
| R795 |
T1352 |
T1351 |
pobj |
afferents,of |
| R796 |
T1353 |
T1352 |
cc |
and,afferents |
| R797 |
T1354 |
T1352 |
conj |
efferents,afferents |
| R798 |
T1355 |
T1350 |
acomp |
unrelated,is |
| R799 |
T1356 |
T1355 |
prep |
to,unrelated |
| R800 |
T1357 |
T1358 |
compound |
hair,cell |
| R801 |
T1358 |
T1359 |
compound |
cell,differentiation |
| R802 |
T1359 |
T1356 |
pobj |
differentiation,to |
| R803 |
T1360 |
T1361 |
mark |
as,detected |
| R804 |
T1361 |
T1350 |
advcl |
detected,is |
| R805 |
T1362 |
T1363 |
neg |
not,cells |
| R806 |
T1363 |
T1361 |
nsubjpass |
cells,detected |
| R807 |
T1364 |
T1363 |
advmod |
even,cells |
| R808 |
T1365 |
T1363 |
amod |
immature,cells |
| R809 |
T1366 |
T1363 |
compound |
hair,cells |
| R810 |
T1367 |
T1361 |
aux |
can,detected |
| R811 |
T1368 |
T1361 |
auxpass |
be,detected |
| R812 |
T1369 |
T1361 |
prep |
at,detected |
| R813 |
T1370 |
T1371 |
amod |
early,stages |
| R814 |
T1371 |
T1369 |
pobj |
stages,at |
| R815 |
T1372 |
T1371 |
amod |
postnatal,stages |
| R816 |
T1373 |
T1361 |
prep |
with,detected |
| R817 |
T1374 |
T1375 |
compound |
MyoVII,immunocytochemistry |
| R818 |
T1375 |
T1373 |
pobj |
immunocytochemistry,with |
| R819 |
T1376 |
T1361 |
prep |
in,detected |
| R820 |
T1377 |
T1378 |
det |
this,part |
| R821 |
T1378 |
T1376 |
pobj |
part,in |
| R822 |
T1379 |
T1378 |
prep |
of,part |
| R823 |
T1380 |
T1381 |
det |
the,cochlea |
| R824 |
T1381 |
T1379 |
pobj |
cochlea,of |
| R825 |
T1382 |
T1350 |
punct |
.,is |
| R826 |
T1384 |
T1385 |
det |
This,retention |
| R827 |
T1385 |
T1386 |
nsubj |
retention,is |
| R828 |
T1387 |
T1385 |
prep |
of,retention |
| R829 |
T1388 |
T1389 |
amod |
apical,neurons |
| R830 |
T1389 |
T1387 |
pobj |
neurons,of |
| R831 |
T1390 |
T1389 |
amod |
spiral,neurons |
| R832 |
T1391 |
T1386 |
advmod |
also,is |
| R833 |
T1392 |
T1393 |
advmod |
largely,unrelated |
| R834 |
T1393 |
T1386 |
acomp |
unrelated,is |
| R835 |
T1394 |
T1393 |
prep |
to,unrelated |
| R836 |
T1395 |
T1394 |
pobj |
neurotrophins,to |
| R837 |
T1396 |
T1397 |
dep |
which,known |
| R838 |
T1397 |
T1395 |
relcl |
known,neurotrophins |
| R839 |
T1398 |
T1397 |
auxpass |
are,known |
| R840 |
T1399 |
T1400 |
aux |
to,reduced |
| R841 |
T1400 |
T1397 |
xcomp |
reduced,known |
| R842 |
T1401 |
T1400 |
auxpass |
be,reduced |
| R843 |
T1402 |
T1400 |
prep |
in,reduced |
| R844 |
T1403 |
T1404 |
poss |
their,expression |
| R845 |
T1404 |
T1402 |
pobj |
expression,in |
| R846 |
T1405 |
T1397 |
prep |
in,known |
| R847 |
T1406 |
T1407 |
amod |
neonatal,rodents |
| R848 |
T1407 |
T1405 |
pobj |
rodents,in |
| R849 |
T1408 |
T1409 |
punct |
[,4 |
| R850 |
T1409 |
T1386 |
parataxis |
4,is |
| R851 |
T1410 |
T1409 |
punct |
],4 |
| R852 |
T1411 |
T1386 |
punct |
.,is |
| R854 |
T1476 |
T1477 |
aux |
To,appreciate |
| R855 |
T1477 |
T1478 |
advcl |
appreciate,want |
| R856 |
T1479 |
T1480 |
det |
the,effects |
| R857 |
T1480 |
T1477 |
dobj |
effects,appreciate |
| R858 |
T1481 |
T1480 |
prep |
of,effects |
| R859 |
T1482 |
T1483 |
det |
the,mutation |
| R860 |
T1483 |
T1481 |
pobj |
mutation,of |
| R861 |
T1484 |
T1483 |
nmod |
Brn3c,mutation |
| R862 |
T1485 |
T1483 |
amod |
null,mutation |
| R863 |
T1486 |
T1480 |
prep |
on,effects |
| R864 |
T1487 |
T1488 |
det |
the,pattern |
| R865 |
T1488 |
T1486 |
pobj |
pattern,on |
| R866 |
T1489 |
T1488 |
prep |
of,pattern |
| R867 |
T1490 |
T1491 |
det |
the,innervation |
| R868 |
T1491 |
T1489 |
pobj |
innervation,of |
| R869 |
T1492 |
T1493 |
amod |
inner,ear |
| R870 |
T1493 |
T1491 |
compound |
ear,innervation |
| R871 |
T1494 |
T1478 |
punct |
", ",want |
| R872 |
T1495 |
T1478 |
nsubj |
we,want |
| R873 |
T1496 |
T1478 |
advmod |
first,want |
| R874 |
T1497 |
T1498 |
aux |
to,present |
| R875 |
T1498 |
T1478 |
xcomp |
present,want |
| R876 |
T1499 |
T1500 |
det |
the,effects |
| R877 |
T1500 |
T1498 |
dobj |
effects,present |
| R878 |
T1501 |
T1500 |
prep |
of,effects |
| R879 |
T1502 |
T1503 |
nmod |
BDNF,mutations |
| R880 |
T1503 |
T1501 |
pobj |
mutations,of |
| R881 |
T1504 |
T1502 |
cc |
and,BDNF |
| R882 |
T1505 |
T1502 |
conj |
NT,BDNF |
| R883 |
T1506 |
T1505 |
punct |
-,NT |
| R884 |
T1507 |
T1505 |
nummod |
3,NT |
| R885 |
T1508 |
T1503 |
amod |
null,mutations |
| R886 |
T1509 |
T1503 |
prep |
at,mutations |
| R887 |
T1510 |
T1509 |
pobj |
birth,at |
| R888 |
T1511 |
T1512 |
punct |
[,14 |
| R889 |
T1512 |
T1478 |
parataxis |
14,want |
| R890 |
T1513 |
T1512 |
nummod |
6,14 |
| R891 |
T1514 |
T1512 |
punct |
",",14 |
| R892 |
T1515 |
T1512 |
nummod |
13,14 |
| R893 |
T1516 |
T1512 |
punct |
",",14 |
| R894 |
T1517 |
T1512 |
punct |
],14 |
| R895 |
T1518 |
T1478 |
punct |
.,want |
| R896 |
T1520 |
T1521 |
amod |
Null,mutants |
| R897 |
T1521 |
T1522 |
nsubj |
mutants,lose |
| R898 |
T1523 |
T1521 |
prep |
of,mutants |
| R899 |
T1524 |
T1523 |
pobj |
BDNF,of |
| R900 |
T1525 |
T1524 |
cc |
or,BDNF |
| R901 |
T1526 |
T1527 |
poss |
its,receptor |
| R902 |
T1527 |
T1524 |
conj |
receptor,BDNF |
| R903 |
T1528 |
T1527 |
appos |
trkB,receptor |
| R904 |
T1529 |
T1530 |
det |
all,innervation |
| R905 |
T1530 |
T1522 |
dobj |
innervation,lose |
| R906 |
T1531 |
T1530 |
prep |
to,innervation |
| R907 |
T1532 |
T1533 |
det |
the,canals |
| R908 |
T1533 |
T1531 |
pobj |
canals,to |
| R909 |
T1534 |
T1533 |
amod |
semicircular,canals |
| R910 |
T1535 |
T1522 |
cc |
and,lose |
| R911 |
T1536 |
T1522 |
conj |
have,lose |
| R912 |
T1537 |
T1538 |
det |
a,innervation |
| R913 |
T1538 |
T1536 |
dobj |
innervation,have |
| R914 |
T1539 |
T1538 |
amod |
reduced,innervation |
| R915 |
T1540 |
T1538 |
prep |
to,innervation |
| R916 |
T1541 |
T1542 |
det |
the,utricle |
| R917 |
T1542 |
T1540 |
pobj |
utricle,to |
| R918 |
T1543 |
T1542 |
punct |
", ",utricle |
| R919 |
T1544 |
T1542 |
conj |
saccule,utricle |
| R920 |
T1545 |
T1544 |
cc |
and,saccule |
| R921 |
T1546 |
T1547 |
amod |
apical,turn |
| R922 |
T1547 |
T1544 |
conj |
turn,saccule |
| R923 |
T1548 |
T1547 |
prep |
of,turn |
| R924 |
T1549 |
T1550 |
det |
the,cochlea |
| R925 |
T1550 |
T1548 |
pobj |
cochlea,of |
| R926 |
T1551 |
T1522 |
punct |
.,lose |
| R927 |
T1553 |
T1554 |
prep |
In,result |
| R928 |
T1555 |
T1553 |
pobj |
contrast,In |
| R929 |
T1556 |
T1554 |
punct |
", ",result |
| R930 |
T1557 |
T1558 |
amod |
null,mutations |
| R931 |
T1558 |
T1554 |
nsubj |
mutations,result |
| R932 |
T1559 |
T1558 |
prep |
of,mutations |
| R933 |
T1560 |
T1561 |
preconj |
either,NT |
| R934 |
T1561 |
T1559 |
pobj |
NT,of |
| R935 |
T1562 |
T1561 |
punct |
-,NT |
| R936 |
T1563 |
T1561 |
nummod |
3,NT |
| R937 |
T1564 |
T1561 |
cc |
or,NT |
| R938 |
T1565 |
T1566 |
poss |
its,receptor |
| R939 |
T1566 |
T1561 |
conj |
receptor,NT |
| R940 |
T1567 |
T1566 |
appos |
trkC,receptor |
| R941 |
T1568 |
T1554 |
prep |
in,result |
| R942 |
T1569 |
T1568 |
pobj |
loss,in |
| R943 |
T1570 |
T1569 |
prep |
of,loss |
| R944 |
T1571 |
T1572 |
amod |
spiral,neurons |
| R945 |
T1572 |
T1570 |
pobj |
neurons,of |
| R946 |
T1573 |
T1569 |
prep |
in,loss |
| R947 |
T1574 |
T1575 |
det |
the,turn |
| R948 |
T1575 |
T1573 |
pobj |
turn,in |
| R949 |
T1576 |
T1575 |
amod |
basal,turn |
| R950 |
T1577 |
T1569 |
prep |
with,loss |
| R951 |
T1578 |
T1577 |
pobj |
formation,with |
| R952 |
T1579 |
T1578 |
prep |
of,formation |
| R953 |
T1580 |
T1581 |
det |
an,bundle |
| R954 |
T1581 |
T1579 |
pobj |
bundle,of |
| R955 |
T1582 |
T1583 |
amod |
inner,spiral |
| R956 |
T1583 |
T1581 |
nmod |
spiral,bundle |
| R957 |
T1584 |
T1581 |
prep |
of,bundle |
| R958 |
T1585 |
T1584 |
pobj |
afferents,of |
| R959 |
T1586 |
T1581 |
acl |
extending,bundle |
| R960 |
T1587 |
T1586 |
prep |
to,extending |
| R961 |
T1588 |
T1589 |
det |
the,tip |
| R962 |
T1589 |
T1587 |
pobj |
tip,to |
| R963 |
T1590 |
T1589 |
amod |
basal,tip |
| R964 |
T1591 |
T1554 |
punct |
.,result |
| R965 |
T1593 |
T1594 |
poss |
Our,hypothesis |
| R966 |
T1594 |
T1596 |
nsubj |
hypothesis,be |
| R967 |
T1595 |
T1594 |
amod |
null,hypothesis |
| R968 |
T1597 |
T1594 |
prep |
for,hypothesis |
| R969 |
T1598 |
T1599 |
det |
this,study |
| R970 |
T1599 |
T1597 |
pobj |
study,for |
| R971 |
T1600 |
T1596 |
aux |
would,be |
| R972 |
T1601 |
T1602 |
mark |
that,show |
| R973 |
T1602 |
T1596 |
ccomp |
show,be |
| R974 |
T1603 |
T1604 |
nmod |
Brn3c,mice |
| R975 |
T1604 |
T1602 |
nsubj |
mice,show |
| R976 |
T1605 |
T1604 |
amod |
null,mice |
| R977 |
T1606 |
T1607 |
amod |
severe,production |
| R978 |
T1607 |
T1602 |
dobj |
production,show |
| R979 |
T1608 |
T1607 |
amod |
compromised,production |
| R980 |
T1609 |
T1607 |
prep |
of,production |
| R981 |
T1610 |
T1611 |
det |
these,neurotrophins |
| R982 |
T1611 |
T1609 |
pobj |
neurotrophins,of |
| R983 |
T1612 |
T1602 |
cc |
and,show |
| R984 |
T1613 |
T1614 |
aux |
should,show |
| R985 |
T1614 |
T1602 |
conj |
show,show |
| R986 |
T1615 |
T1614 |
advmod |
therefore,show |
| R987 |
T1616 |
T1617 |
det |
a,pattern |
| R988 |
T1617 |
T1614 |
dobj |
pattern,show |
| R989 |
T1618 |
T1617 |
amod |
comparable,pattern |
| R990 |
T1619 |
T1617 |
prep |
of,pattern |
| R991 |
T1620 |
T1621 |
compound |
nerve,fiber |
| R992 |
T1621 |
T1622 |
compound |
fiber,loss |
| R993 |
T1622 |
T1619 |
pobj |
loss,of |
| R994 |
T1623 |
T1596 |
punct |
.,be |
| R4 |
T190 |
T192 |
nsubj |
mice,show |
| R7 |
T194 |
T196 |
nmod |
term,retention |
| R21 |
T213 |
T211 |
pobj |
mutants,of |
| R24 |
T216 |
T210 |
dobj |
cells,develop |
| R31 |
T223 |
T221 |
pobj |
markers,by |
| R41 |
T234 |
T236 |
nsubj |
development,lead |
| R49 |
T243 |
T241 |
pobj |
expression,to |
| R80 |
T280 |
T276 |
attr |
expression,is |
| R98 |
T298 |
T296 |
dobj |
reduction,shows |
| R107 |
T307 |
T305 |
dobj |
mutations,resembles |
| R135 |
T339 |
T341 |
amod |
positive,cells |
| R143 |
T347 |
T345 |
pobj |
cochlea,in |
| R156 |
T362 |
T360 |
pobj |
cells,of |
| R173 |
T381 |
T376 |
parataxis |
%,loss |
| R188 |
T396 |
T392 |
parataxis |
%,neurons |
| R217 |
T427 |
T425 |
pobj |
apex,to |
| R223 |
T433 |
T430 |
dobj |
cells,lacks |
| R250 |
T465 |
T467 |
nsubj |
expression,suffices |
| R264 |
T480 |
T478 |
dobj |
neurons,support |
| R276 |
T493 |
T495 |
nsubj |
nature,remains |