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PMC:149350 / 8509-9301
Annnotations
craft-ca-core-dev
Below, discontinuous spans are shown in the chain model. You can change it to the bag model.
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T2248 | 187-201 | CHEBI:64276 | denotes | glutaraldehyde |
T2249 | 217-234 | CHEBI:62956 | denotes | sodium cacodylate |
T2250 | 295-299 | CHEBI:9754 | denotes | Tris |
T2251 | 323-327 | CHEBI:9754 | denotes | Tris |
T2252 | 336-340 | CHEBI:26710 | denotes | NaCl |
T2253 | 364-369 | CHEBI:18059 | denotes | lipid |
T2254 | 370-379 | GO:0016020 | denotes | membranes |
T2255 | 408-474 | CHEBI:52029 | denotes | 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate |
T2256 | 477-480 | CHEBI:52029 | denotes | DiI |
T2257 | 482-488 | CHEBI:52029 | denotes | DiIC18 |
T2258 | 583-587 | CHEBI:9754 | denotes | Tris |
T2259 | 609-616 | GO:0005634 | denotes | nucleic |
craft-sa-dev
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T2292 | 0-6 | NNS | denotes | Lenses |
T2293 | 22-31 | VBN | denotes | sectioned |
T2294 | 7-11 | VBD | denotes | were |
T2295 | 12-21 | NN | denotes | vibratome |
T2296 | 32-36 | IN | denotes | into |
T2297 | 37-40 | CD | denotes | 100 |
T2298 | 41-44 | CD | denotes | 200 |
T2299 | 40-41 | SYM | denotes | – |
T2300 | 45-47 | NN | denotes | μm |
T2301 | 48-53 | JJ | denotes | thick |
T2302 | 54-62 | NNS | denotes | sections |
T2303 | 63-68 | IN | denotes | along |
T2304 | 69-72 | DT | denotes | the |
T2305 | 119-123 | NNS | denotes | axes |
T2306 | 73-80 | JJ | denotes | optical |
T2307 | 81-82 | -LRB- | denotes | ( |
T2308 | 82-90 | JJ | denotes | anterior |
T2309 | 91-93 | IN | denotes | to |
T2310 | 94-103 | JJ | denotes | posterior |
T2311 | 103-104 | -RRB- | denotes | ) |
T2312 | 105-107 | CC | denotes | or |
T2313 | 108-118 | JJ | denotes | equatorial |
T2314 | 123-124 | . | denotes | . |
T2315 | 124-350 | sentence | denotes | Thick sections were fixed in 2% (w/v) paraformaldehyde and 2% glutaraldehyde (v/v) in 0.1 M sodium cacodylate buffer pH 7.2 at RT for 24 hrs then washed several times in Tris buffered saline (0.5 M Tris, 150 mM NaCl, pH 7.4). |
T2316 | 125-130 | JJ | denotes | Thick |
T2317 | 131-139 | NNS | denotes | sections |
T2318 | 145-150 | VBN | denotes | fixed |
T2319 | 140-144 | VBD | denotes | were |
T2320 | 151-153 | IN | denotes | in |
T2321 | 154-155 | CD | denotes | 2 |
T2322 | 155-156 | NN | denotes | % |
T2323 | 163-179 | NN | denotes | paraformaldehyde |
T2324 | 157-158 | -LRB- | denotes | ( |
T2325 | 158-159 | NN | denotes | w |
T2326 | 159-160 | SYM | denotes | / |
T2327 | 160-161 | NN | denotes | v |
T2328 | 161-162 | -RRB- | denotes | ) |
T2329 | 180-183 | CC | denotes | and |
T2330 | 184-185 | CD | denotes | 2 |
T2331 | 185-186 | NN | denotes | % |
T2332 | 187-201 | NN | denotes | glutaraldehyde |
T2333 | 202-203 | -LRB- | denotes | ( |
T2334 | 203-204 | NN | denotes | v |
T2335 | 204-205 | SYM | denotes | / |
T2336 | 205-206 | NN | denotes | v |
T2337 | 206-207 | -RRB- | denotes | ) |
T2338 | 208-210 | IN | denotes | in |
T2339 | 211-214 | CD | denotes | 0.1 |
T2340 | 215-216 | NN | denotes | M |
T2341 | 235-241 | NN | denotes | buffer |
T2342 | 217-223 | NN | denotes | sodium |
T2343 | 224-234 | NN | denotes | cacodylate |
T2344 | 242-244 | NN | denotes | pH |
T2345 | 245-248 | CD | denotes | 7.2 |
T2346 | 249-251 | IN | denotes | at |
T2347 | 252-254 | NN | denotes | RT |
T2348 | 255-258 | IN | denotes | for |
T2349 | 259-261 | CD | denotes | 24 |
T2350 | 262-265 | NNS | denotes | hrs |
T2351 | 266-270 | RB | denotes | then |
T2352 | 271-277 | VBD | denotes | washed |
T2353 | 278-285 | JJ | denotes | several |
T2354 | 286-291 | NNS | denotes | times |
T2355 | 292-294 | IN | denotes | in |
T2356 | 295-299 | NNP | denotes | Tris |
T2357 | 300-308 | VBN | denotes | buffered |
T2358 | 309-315 | NN | denotes | saline |
T2359 | 316-317 | -LRB- | denotes | ( |
T2360 | 342-344 | NN | denotes | pH |
T2361 | 317-320 | CD | denotes | 0.5 |
T2362 | 321-322 | NN | denotes | M |
T2363 | 323-327 | NNP | denotes | Tris |
T2364 | 327-329 | , | denotes | , |
T2365 | 329-332 | CD | denotes | 150 |
T2366 | 333-335 | NN | denotes | mM |
T2367 | 336-340 | NN | denotes | NaCl |
T2368 | 340-342 | , | denotes | , |
T2369 | 345-348 | CD | denotes | 7.4 |
T2370 | 348-349 | -RRB- | denotes | ) |
T2371 | 349-350 | . | denotes | . |
T2372 | 350-537 | sentence | denotes | To visualize lipid membranes, sections were stained with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate ('DiI';DiIC18; Molecular Probes) as previously described [24]. |
T2373 | 351-353 | TO | denotes | To |
T2374 | 354-363 | VB | denotes | visualize |
T2375 | 395-402 | VBN | denotes | stained |
T2376 | 364-369 | NN | denotes | lipid |
T2377 | 370-379 | NNS | denotes | membranes |
T2378 | 379-381 | , | denotes | , |
T2379 | 381-389 | NNS | denotes | sections |
T2380 | 390-394 | VBD | denotes | were |
T2381 | 403-407 | IN | denotes | with |
T2382 | 408-409 | CD | denotes | 1 |
T2383 | 413-424 | NN | denotes | dioctadecyl |
T2384 | 409-410 | , | denotes | , |
T2385 | 410-411 | CD | denotes | 1 |
T2386 | 411-412 | SYM | denotes | ' |
T2387 | 412-413 | HYPH | denotes | - |
T2388 | 424-425 | HYPH | denotes | - |
T2389 | 425-426 | CD | denotes | 3 |
T2390 | 426-427 | , | denotes | , |
T2391 | 427-428 | CD | denotes | 3 |
T2392 | 463-474 | NN | denotes | perchlorate |
T2393 | 428-429 | , | denotes | , |
T2394 | 429-430 | CD | denotes | 3 |
T2395 | 430-431 | SYM | denotes | ' |
T2396 | 431-432 | , | denotes | , |
T2397 | 432-433 | CD | denotes | 3 |
T2398 | 433-434 | SYM | denotes | ' |
T2399 | 434-435 | HYPH | denotes | - |
T2400 | 435-462 | JJ | denotes | tetramethylindocarbocyanine |
T2401 | 475-476 | -LRB- | denotes | ( |
T2402 | 500-506 | NNP | denotes | Probes |
T2403 | 476-477 | `` | denotes | ' |
T2404 | 477-480 | NN | denotes | DiI |
T2405 | 480-481 | '' | denotes | ' |
T2406 | 481-482 | : | denotes | ; |
T2407 | 482-488 | NN | denotes | DiIC18 |
T2408 | 488-489 | : | denotes | ; |
T2409 | 490-499 | NNP | denotes | Molecular |
T2410 | 506-507 | -RRB- | denotes | ) |
T2411 | 508-510 | IN | denotes | as |
T2412 | 522-531 | VBN | denotes | described |
T2413 | 511-521 | RB | denotes | previously |
T2414 | 532-533 | -LRB- | denotes | [ |
T2415 | 533-535 | CD | denotes | 24 |
T2416 | 535-536 | -RRB- | denotes | ] |
T2417 | 536-537 | . | denotes | . |
T2418 | 537-792 | sentence | denotes | Sections were then washed several times with Tris buffered saline, and nucleic acids were stained by incubating sections in TBS containing 1 μM SYTOX Green (Molecular Probes) for 10 min at RT followed by several washes with TBS prior to confocal imaging. |
T2419 | 538-546 | NNS | denotes | Sections |
T2420 | 557-563 | VBN | denotes | washed |
T2421 | 547-551 | VBD | denotes | were |
T2422 | 552-556 | RB | denotes | then |
T2423 | 564-571 | JJ | denotes | several |
T2424 | 572-577 | NNS | denotes | times |
T2425 | 578-582 | IN | denotes | with |
T2426 | 583-587 | NNP | denotes | Tris |
T2427 | 588-596 | VBN | denotes | buffered |
T2428 | 597-603 | NN | denotes | saline |
T2429 | 603-605 | , | denotes | , |
T2430 | 605-608 | CC | denotes | and |
T2431 | 609-616 | JJ | denotes | nucleic |
T2432 | 617-622 | NNS | denotes | acids |
T2433 | 628-635 | VBN | denotes | stained |
T2434 | 623-627 | VBD | denotes | were |
T2435 | 636-638 | IN | denotes | by |
T2436 | 639-649 | VBG | denotes | incubating |
T2437 | 650-658 | NNS | denotes | sections |
T2438 | 659-661 | IN | denotes | in |
T2439 | 662-665 | NN | denotes | TBS |
T2440 | 666-676 | VBG | denotes | containing |
T2441 | 677-678 | CD | denotes | 1 |
T2442 | 679-681 | NN | denotes | μM |
T2443 | 688-693 | NN | denotes | Green |
T2444 | 682-687 | NN | denotes | SYTOX |
T2445 | 694-695 | -LRB- | denotes | ( |
T2446 | 705-711 | NNP | denotes | Probes |
T2447 | 695-704 | JJ | denotes | Molecular |
T2448 | 711-712 | -RRB- | denotes | ) |
T2449 | 713-716 | IN | denotes | for |
T2450 | 717-719 | CD | denotes | 10 |
T2451 | 720-723 | NN | denotes | min |
T2452 | 724-726 | IN | denotes | at |
T2453 | 727-729 | NN | denotes | RT |
T2454 | 730-738 | VBN | denotes | followed |
T2455 | 739-741 | IN | denotes | by |
T2456 | 742-749 | JJ | denotes | several |
T2457 | 750-756 | NNS | denotes | washes |
T2458 | 757-761 | IN | denotes | with |
T2459 | 762-765 | NN | denotes | TBS |
T2460 | 766-771 | RB | denotes | prior |
T2461 | 772-774 | IN | denotes | to |
T2462 | 775-783 | JJ | denotes | confocal |
T2463 | 784-791 | NN | denotes | imaging |
T2464 | 791-792 | . | denotes | . |
R1539 | T2292 | T2293 | nsubjpass | Lenses,sectioned |
R1540 | T2294 | T2293 | auxpass | were,sectioned |
R1541 | T2295 | T2293 | dep | vibratome,sectioned |
R1542 | T2296 | T2293 | prep | into,sectioned |
R1543 | T2297 | T2298 | quantmod | 100,200 |
R1544 | T2298 | T2300 | nummod | 200,μm |
R1545 | T2299 | T2298 | punct | –,200 |
R1546 | T2300 | T2301 | npadvmod | μm,thick |
R1547 | T2301 | T2302 | amod | thick,sections |
R1548 | T2302 | T2296 | pobj | sections,into |
R1549 | T2303 | T2293 | prep | along,sectioned |
R1550 | T2304 | T2305 | det | the,axes |
R1551 | T2305 | T2303 | pobj | axes,along |
R1552 | T2306 | T2305 | amod | optical,axes |
R1553 | T2307 | T2308 | punct | (,anterior |
R1554 | T2308 | T2306 | parataxis | anterior,optical |
R1555 | T2309 | T2308 | prep | to,anterior |
R1556 | T2310 | T2309 | amod | posterior,to |
R1557 | T2311 | T2308 | punct | ),anterior |
R1558 | T2312 | T2306 | cc | or,optical |
R1559 | T2313 | T2306 | conj | equatorial,optical |
R1560 | T2314 | T2293 | punct | .,sectioned |
R1561 | T2316 | T2317 | amod | Thick,sections |
R1562 | T2317 | T2318 | nsubjpass | sections,fixed |
R1563 | T2319 | T2318 | auxpass | were,fixed |
R1564 | T2320 | T2318 | prep | in,fixed |
R1565 | T2321 | T2322 | nummod | 2,% |
R1566 | T2322 | T2323 | nmod | %,paraformaldehyde |
R1567 | T2323 | T2320 | pobj | paraformaldehyde,in |
R1568 | T2324 | T2325 | punct | (,w |
R1569 | T2325 | T2322 | parataxis | w,% |
R1570 | T2326 | T2327 | punct | /,v |
R1571 | T2327 | T2325 | prep | v,w |
R1572 | T2328 | T2325 | punct | ),w |
R1573 | T2329 | T2323 | cc | and,paraformaldehyde |
R1574 | T2330 | T2331 | nummod | 2,% |
R1575 | T2331 | T2332 | compound | %,glutaraldehyde |
R1576 | T2332 | T2323 | conj | glutaraldehyde,paraformaldehyde |
R1577 | T2333 | T2334 | punct | (,v |
R1578 | T2334 | T2332 | parataxis | v,glutaraldehyde |
R1579 | T2335 | T2336 | punct | /,v |
R1580 | T2336 | T2334 | prep | v,v |
R1581 | T2337 | T2334 | punct | ),v |
R1582 | T2338 | T2323 | prep | in,paraformaldehyde |
R1583 | T2339 | T2340 | nummod | 0.1,M |
R1584 | T2340 | T2341 | compound | M,buffer |
R1585 | T2341 | T2338 | pobj | buffer,in |
R1586 | T2342 | T2343 | compound | sodium,cacodylate |
R1587 | T2343 | T2341 | compound | cacodylate,buffer |
R1588 | T2344 | T2323 | npadvmod | pH,paraformaldehyde |
R1589 | T2345 | T2344 | nummod | 7.2,pH |
R1590 | T2346 | T2318 | prep | at,fixed |
R1591 | T2347 | T2346 | pobj | RT,at |
R1592 | T2348 | T2318 | prep | for,fixed |
R1593 | T2349 | T2350 | nummod | 24,hrs |
R1594 | T2350 | T2348 | pobj | hrs,for |
R1595 | T2351 | T2352 | advmod | then,washed |
R1596 | T2352 | T2318 | dep | washed,fixed |
R1597 | T2353 | T2354 | amod | several,times |
R1598 | T2354 | T2352 | npadvmod | times,washed |
R1599 | T2355 | T2352 | prep | in,washed |
R1600 | T2356 | T2357 | npadvmod | Tris,buffered |
R1601 | T2357 | T2358 | amod | buffered,saline |
R1602 | T2358 | T2355 | pobj | saline,in |
R1603 | T2359 | T2360 | punct | (,pH |
R1604 | T2360 | T2358 | parataxis | pH,saline |
R1605 | T2361 | T2362 | nummod | 0.5,M |
R1606 | T2362 | T2363 | compound | M,Tris |
R1607 | T2363 | T2360 | dep | Tris,pH |
R1608 | T2364 | T2360 | punct | ", ",pH |
R1609 | T2365 | T2366 | nummod | 150,mM |
R1610 | T2366 | T2367 | compound | mM,NaCl |
R1611 | T2367 | T2360 | dep | NaCl,pH |
R1612 | T2368 | T2360 | punct | ", ",pH |
R1613 | T2369 | T2360 | nummod | 7.4,pH |
R1614 | T2370 | T2360 | punct | ),pH |
R1615 | T2371 | T2318 | punct | .,fixed |
R1616 | T2373 | T2374 | aux | To,visualize |
R1617 | T2374 | T2375 | advcl | visualize,stained |
R1618 | T2376 | T2377 | compound | lipid,membranes |
R1619 | T2377 | T2374 | dobj | membranes,visualize |
R1620 | T2378 | T2375 | punct | ", ",stained |
R1621 | T2379 | T2375 | nsubjpass | sections,stained |
R1622 | T2380 | T2375 | auxpass | were,stained |
R1623 | T2381 | T2375 | prep | with,stained |
R1624 | T2382 | T2383 | nummod | 1,dioctadecyl |
R1625 | T2383 | T2381 | pobj | dioctadecyl,with |
R1626 | T2384 | T2382 | punct | ",",1 |
R1627 | T2385 | T2382 | appos | 1,1 |
R1628 | T2386 | T2385 | punct | ',1 |
R1629 | T2387 | T2383 | punct | -,dioctadecyl |
R1630 | T2388 | T2383 | punct | -,dioctadecyl |
R1631 | T2389 | T2383 | nummod | 3,dioctadecyl |
R1632 | T2390 | T2383 | punct | ",",dioctadecyl |
R1633 | T2391 | T2392 | nummod | 3,perchlorate |
R1634 | T2392 | T2383 | appos | perchlorate,dioctadecyl |
R1635 | T2393 | T2391 | punct | ",",3 |
R1636 | T2394 | T2391 | appos | 3,3 |
R1637 | T2395 | T2394 | punct | ',3 |
R1638 | T2396 | T2391 | punct | ",",3 |
R1639 | T2397 | T2391 | appos | 3,3 |
R1640 | T2398 | T2397 | punct | ',3 |
R1641 | T2399 | T2391 | punct | -,3 |
R1642 | T2400 | T2391 | amod | tetramethylindocarbocyanine,3 |
R1643 | T2401 | T2402 | punct | (,Probes |
R1644 | T2402 | T2383 | parataxis | Probes,dioctadecyl |
R1645 | T2403 | T2402 | punct | ',Probes |
R1646 | T2404 | T2402 | dep | DiI,Probes |
R1647 | T2405 | T2402 | punct | ',Probes |
R1648 | T2406 | T2402 | punct | ;,Probes |
R1649 | T2407 | T2402 | dep | DiIC18,Probes |
R1650 | T2408 | T2402 | punct | ;,Probes |
R1651 | T2409 | T2402 | compound | Molecular,Probes |
R1652 | T2410 | T2402 | punct | ),Probes |
R1653 | T2411 | T2412 | mark | as,described |
R1654 | T2412 | T2375 | advcl | described,stained |
R1655 | T2413 | T2412 | advmod | previously,described |
R1656 | T2414 | T2415 | punct | [,24 |
R1657 | T2415 | T2375 | parataxis | 24,stained |
R1658 | T2416 | T2415 | punct | ],24 |
R1659 | T2417 | T2375 | punct | .,stained |
R1660 | T2419 | T2420 | nsubjpass | Sections,washed |
R1661 | T2421 | T2420 | auxpass | were,washed |
R1662 | T2422 | T2420 | advmod | then,washed |
R1663 | T2423 | T2424 | amod | several,times |
R1664 | T2424 | T2420 | npadvmod | times,washed |
R1665 | T2425 | T2420 | prep | with,washed |
R1666 | T2426 | T2427 | npadvmod | Tris,buffered |
R1667 | T2427 | T2428 | amod | buffered,saline |
R1668 | T2428 | T2425 | pobj | saline,with |
R1669 | T2429 | T2420 | punct | ", ",washed |
R1670 | T2430 | T2420 | cc | and,washed |
R1671 | T2431 | T2432 | amod | nucleic,acids |
R1672 | T2432 | T2433 | nsubjpass | acids,stained |
R1673 | T2433 | T2420 | conj | stained,washed |
R1674 | T2434 | T2433 | auxpass | were,stained |
R1675 | T2435 | T2433 | prep | by,stained |
R1676 | T2436 | T2435 | pcomp | incubating,by |
R1677 | T2437 | T2436 | dobj | sections,incubating |
R1678 | T2438 | T2436 | prep | in,incubating |
R1679 | T2439 | T2438 | pobj | TBS,in |
R1680 | T2440 | T2439 | acl | containing,TBS |
R1681 | T2441 | T2442 | nummod | 1,μM |
R1682 | T2442 | T2443 | compound | μM,Green |
R1683 | T2443 | T2440 | dobj | Green,containing |
R1684 | T2444 | T2443 | compound | SYTOX,Green |
R1685 | T2445 | T2446 | punct | (,Probes |
R1686 | T2446 | T2440 | parataxis | Probes,containing |
R1687 | T2447 | T2446 | amod | Molecular,Probes |
R1688 | T2448 | T2446 | punct | ),Probes |
R1689 | T2449 | T2436 | prep | for,incubating |
R1690 | T2450 | T2451 | nummod | 10,min |
R1691 | T2451 | T2449 | pobj | min,for |
R1692 | T2452 | T2436 | prep | at,incubating |
R1693 | T2453 | T2452 | pobj | RT,at |
R1694 | T2454 | T2436 | advcl | followed,incubating |
R1695 | T2455 | T2454 | agent | by,followed |
R1696 | T2456 | T2457 | amod | several,washes |
R1697 | T2457 | T2455 | pobj | washes,by |
R1698 | T2458 | T2457 | prep | with,washes |
R1699 | T2459 | T2458 | pobj | TBS,with |
R1700 | T2460 | T2457 | advmod | prior,washes |
R1701 | T2461 | T2460 | prep | to,prior |
R1702 | T2462 | T2463 | amod | confocal,imaging |
R1703 | T2463 | T2461 | pobj | imaging,to |
R1704 | T2464 | T2433 | punct | .,stained |
craft-ca-core-ex-dev
Below, discontinuous spans are shown in the chain model. You can change it to the bag model.
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T2261 | 0-6 | UBERON_EXT:lens | denotes | Lenses |
T2262 | 163-179 | CHEBI_EXT:paraformaldehye | denotes | paraformaldehyde |
T2263 | 187-201 | CHEBI:64276 | denotes | glutaraldehyde |
T2264 | 217-234 | CHEBI:62956 | denotes | sodium cacodylate |
T2265 | 235-241 | CHEBI_CHMO_EXT:buffer_solution | denotes | buffer |
T2266 | 295-299 | CHEBI:9754 | denotes | Tris |
T2267 | 300-308 | CHEBI_CHMO_EXT:buffer_process | denotes | buffered |
T2268 | 309-315 | CHEBI_EXT:saline_solution | denotes | saline |
T2269 | 323-327 | CHEBI:9754 | denotes | Tris |
T2270 | 336-340 | CHEBI:26710 | denotes | NaCl |
T2271 | 364-369 | CHEBI:18059 | denotes | lipid |
T2272 | 370-379 | GO:0016020 | denotes | membranes |
T2273 | 408-474 | CHEBI:52029 | denotes | 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate |
T2274 | 477-480 | CHEBI:52029 | denotes | DiI |
T2275 | 482-488 | CHEBI:52029 | denotes | DiIC18 |
T2276 | 490-499 | CHEBI_EXT:polyatomic_entity_or_group | denotes | Molecular |
T2277 | 500-506 | CHEBI_SO_EXT:molecular_probe | denotes | Probes |
T2278 | 583-587 | CHEBI:9754 | denotes | Tris |
T2279 | 588-596 | CHEBI_CHMO_EXT:buffer_process | denotes | buffered |
T2280 | 597-603 | CHEBI_EXT:saline_solution | denotes | saline |
T2281 | 609-616 | GO:0005634 | denotes | nucleic |
T2282 | 609-622 | CHEBI_SO_EXT:nucleic_acid | denotes | nucleic acids |
T2283 | 695-704 | CHEBI_EXT:polyatomic_entity_or_group | denotes | Molecular |
T2284 | 705-711 | CHEBI_SO_EXT:molecular_probe | denotes | Probes |