Id |
Subject |
Object |
Predicate |
Lexical cue |
T1951 |
9-15 |
NNS |
denotes |
Lenses |
T1952 |
15-87 |
sentence |
denotes |
The wild type mouse strain used in this study was the 129SvEvTac mouse. |
T1953 |
16-19 |
DT |
denotes |
The |
T1954 |
36-42 |
NN |
denotes |
strain |
T1955 |
20-24 |
JJ |
denotes |
wild |
T1956 |
25-29 |
NN |
denotes |
type |
T1957 |
30-35 |
NN |
denotes |
mouse |
T1958 |
62-65 |
VBD |
denotes |
was |
T1959 |
43-47 |
VBN |
denotes |
used |
T1960 |
48-50 |
IN |
denotes |
in |
T1961 |
51-55 |
DT |
denotes |
this |
T1962 |
56-61 |
NN |
denotes |
study |
T1963 |
66-69 |
DT |
denotes |
the |
T1964 |
81-86 |
NN |
denotes |
mouse |
T1965 |
70-80 |
NN |
denotes |
129SvEvTac |
T1966 |
86-87 |
. |
denotes |
. |
T1967 |
87-169 |
sentence |
denotes |
Lenses examined were from 5(8 lenses), 46(8 lenses) and 72(6 lenses) wk old mice. |
T1968 |
88-94 |
NNS |
denotes |
Lenses |
T1969 |
104-108 |
VBD |
denotes |
were |
T1970 |
95-103 |
VBN |
denotes |
examined |
T1971 |
109-113 |
IN |
denotes |
from |
T1972 |
114-115 |
CD |
denotes |
5 |
T1973 |
157-159 |
NN |
denotes |
wk |
T1974 |
115-116 |
-LRB- |
denotes |
( |
T1975 |
118-124 |
NNS |
denotes |
lenses |
T1976 |
116-117 |
CD |
denotes |
8 |
T1977 |
124-125 |
-RRB- |
denotes |
) |
T1978 |
125-127 |
, |
denotes |
, |
T1979 |
127-129 |
CD |
denotes |
46 |
T1980 |
129-130 |
-LRB- |
denotes |
( |
T1981 |
132-138 |
NNS |
denotes |
lenses |
T1982 |
130-131 |
CD |
denotes |
8 |
T1983 |
138-139 |
-RRB- |
denotes |
) |
T1984 |
140-143 |
CC |
denotes |
and |
T1985 |
144-146 |
CD |
denotes |
72 |
T1986 |
146-147 |
-LRB- |
denotes |
( |
T1987 |
149-155 |
NNS |
denotes |
lenses |
T1988 |
147-148 |
CD |
denotes |
6 |
T1989 |
155-156 |
-RRB- |
denotes |
) |
T1990 |
160-163 |
JJ |
denotes |
old |
T1991 |
164-168 |
NNS |
denotes |
mice |
T1992 |
168-169 |
. |
denotes |
. |
T1993 |
169-286 |
sentence |
denotes |
AlphaA/BKO was generated by cross breeding alphaAKO-127 [22] and alphaBKO-168 mice [23], also in a 129Sv background. |
T1994 |
170-176 |
NN |
denotes |
AlphaA |
T1995 |
177-180 |
NN |
denotes |
BKO |
T1996 |
176-177 |
HYPH |
denotes |
/ |
T1997 |
185-194 |
VBN |
denotes |
generated |
T1998 |
181-184 |
VBD |
denotes |
was |
T1999 |
195-197 |
IN |
denotes |
by |
T2000 |
198-203 |
NN |
denotes |
cross |
T2001 |
204-212 |
VBG |
denotes |
breeding |
T2002 |
213-221 |
NN |
denotes |
alphaAKO |
T2003 |
222-225 |
NN |
denotes |
127 |
T2004 |
221-222 |
HYPH |
denotes |
- |
T2005 |
248-252 |
NNS |
denotes |
mice |
T2006 |
226-227 |
-LRB- |
denotes |
[ |
T2007 |
227-229 |
CD |
denotes |
22 |
T2008 |
229-230 |
-RRB- |
denotes |
] |
T2009 |
231-234 |
CC |
denotes |
and |
T2010 |
235-243 |
NN |
denotes |
alphaBKO |
T2011 |
244-247 |
NN |
denotes |
168 |
T2012 |
243-244 |
HYPH |
denotes |
- |
T2013 |
253-254 |
-LRB- |
denotes |
[ |
T2014 |
254-256 |
CD |
denotes |
23 |
T2015 |
256-257 |
-RRB- |
denotes |
] |
T2016 |
257-259 |
, |
denotes |
, |
T2017 |
259-263 |
RB |
denotes |
also |
T2018 |
264-266 |
IN |
denotes |
in |
T2019 |
267-268 |
DT |
denotes |
a |
T2020 |
275-285 |
NN |
denotes |
background |
T2021 |
269-274 |
NN |
denotes |
129Sv |
T2022 |
285-286 |
. |
denotes |
. |
T2023 |
286-336 |
sentence |
denotes |
These mice also lack the HSPB2 gene product [23]. |
T2024 |
287-292 |
DT |
denotes |
These |
T2025 |
293-297 |
NNS |
denotes |
mice |
T2026 |
303-307 |
VBP |
denotes |
lack |
T2027 |
298-302 |
RB |
denotes |
also |
T2028 |
308-311 |
DT |
denotes |
the |
T2029 |
323-330 |
NN |
denotes |
product |
T2030 |
312-317 |
NN |
denotes |
HSPB2 |
T2031 |
318-322 |
NN |
denotes |
gene |
T2032 |
331-332 |
-LRB- |
denotes |
[ |
T2033 |
332-334 |
CD |
denotes |
23 |
T2034 |
334-335 |
-RRB- |
denotes |
] |
T2035 |
335-336 |
. |
denotes |
. |
T2036 |
336-410 |
sentence |
denotes |
Lenses examined were from 5 wk (6 lenses) and 54 wk (16 lenses) old mice. |
T2037 |
337-343 |
NNS |
denotes |
Lenses |
T2038 |
353-357 |
VBD |
denotes |
were |
T2039 |
344-352 |
VBN |
denotes |
examined |
T2040 |
358-362 |
IN |
denotes |
from |
T2041 |
363-364 |
CD |
denotes |
5 |
T2042 |
365-367 |
NN |
denotes |
wk |
T2043 |
401-404 |
JJ |
denotes |
old |
T2044 |
368-369 |
-LRB- |
denotes |
( |
T2045 |
371-377 |
NNS |
denotes |
lenses |
T2046 |
369-370 |
CD |
denotes |
6 |
T2047 |
377-378 |
-RRB- |
denotes |
) |
T2048 |
379-382 |
CC |
denotes |
and |
T2049 |
383-385 |
CD |
denotes |
54 |
T2050 |
386-388 |
NN |
denotes |
wk |
T2051 |
389-390 |
-LRB- |
denotes |
( |
T2052 |
393-399 |
NNS |
denotes |
lenses |
T2053 |
390-392 |
CD |
denotes |
16 |
T2054 |
399-400 |
-RRB- |
denotes |
) |
T2055 |
405-409 |
NNS |
denotes |
mice |
T2056 |
409-410 |
. |
denotes |
. |
T2057 |
410-579 |
sentence |
denotes |
All animals were treated in accordance with the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research. |
T2058 |
411-414 |
DT |
denotes |
All |
T2059 |
415-422 |
NNS |
denotes |
animals |
T2060 |
428-435 |
VBN |
denotes |
treated |
T2061 |
423-427 |
VBD |
denotes |
were |
T2062 |
436-438 |
IN |
denotes |
in |
T2063 |
439-449 |
NN |
denotes |
accordance |
T2064 |
450-454 |
IN |
denotes |
with |
T2065 |
455-458 |
DT |
denotes |
the |
T2066 |
512-521 |
NNP |
denotes |
Statement |
T2067 |
459-470 |
NNP |
denotes |
Association |
T2068 |
471-474 |
IN |
denotes |
for |
T2069 |
475-483 |
NNP |
denotes |
Research |
T2070 |
484-486 |
IN |
denotes |
in |
T2071 |
487-493 |
NNP |
denotes |
Vision |
T2072 |
494-497 |
CC |
denotes |
and |
T2073 |
498-511 |
NNP |
denotes |
Ophthalmology |
T2074 |
522-525 |
IN |
denotes |
for |
T2075 |
526-529 |
DT |
denotes |
the |
T2076 |
530-533 |
NNP |
denotes |
Use |
T2077 |
534-536 |
IN |
denotes |
of |
T2078 |
537-544 |
NNPS |
denotes |
Animals |
T2079 |
545-547 |
IN |
denotes |
in |
T2080 |
548-558 |
NNP |
denotes |
Ophthalmic |
T2081 |
570-578 |
NNP |
denotes |
Research |
T2082 |
559-562 |
CC |
denotes |
and |
T2083 |
563-569 |
NNP |
denotes |
Vision |
T2084 |
578-579 |
. |
denotes |
. |
T2085 |
579-640 |
sentence |
denotes |
Immediately after euthanizing animals, eyes were enucleated. |
T2086 |
580-591 |
RB |
denotes |
Immediately |
T2087 |
592-597 |
IN |
denotes |
after |
T2088 |
629-639 |
VBN |
denotes |
enucleated |
T2089 |
598-609 |
VBG |
denotes |
euthanizing |
T2090 |
610-617 |
NNS |
denotes |
animals |
T2091 |
617-619 |
, |
denotes |
, |
T2092 |
619-623 |
NNS |
denotes |
eyes |
T2093 |
624-628 |
VBD |
denotes |
were |
T2094 |
639-640 |
. |
denotes |
. |
T2095 |
640-911 |
sentence |
denotes |
A scalpel blade was then used to make a small incision into the anterior chamber near the equator and then both eyes from individual animals were immersed in 5 ml fixative (2% (w/v) paraformaldehyde and 2% glutaraldehyde (v/v) in 0.1 M sodium cacodylate buffer, pH 7.2). |
T2096 |
641-642 |
DT |
denotes |
A |
T2097 |
651-656 |
NN |
denotes |
blade |
T2098 |
643-650 |
NN |
denotes |
scalpel |
T2099 |
666-670 |
VBN |
denotes |
used |
T2100 |
657-660 |
VBD |
denotes |
was |
T2101 |
661-665 |
RB |
denotes |
then |
T2102 |
671-673 |
TO |
denotes |
to |
T2103 |
674-678 |
VB |
denotes |
make |
T2104 |
679-680 |
DT |
denotes |
a |
T2105 |
687-695 |
NN |
denotes |
incision |
T2106 |
681-686 |
JJ |
denotes |
small |
T2107 |
696-700 |
IN |
denotes |
into |
T2108 |
701-704 |
DT |
denotes |
the |
T2109 |
714-721 |
NN |
denotes |
chamber |
T2110 |
705-713 |
JJ |
denotes |
anterior |
T2111 |
722-726 |
IN |
denotes |
near |
T2112 |
727-730 |
DT |
denotes |
the |
T2113 |
731-738 |
NN |
denotes |
equator |
T2114 |
739-742 |
CC |
denotes |
and |
T2115 |
743-747 |
RB |
denotes |
then |
T2116 |
787-795 |
VBN |
denotes |
immersed |
T2117 |
748-752 |
DT |
denotes |
both |
T2118 |
753-757 |
NNS |
denotes |
eyes |
T2119 |
758-762 |
IN |
denotes |
from |
T2120 |
763-773 |
JJ |
denotes |
individual |
T2121 |
774-781 |
NNS |
denotes |
animals |
T2122 |
782-786 |
VBD |
denotes |
were |
T2123 |
796-798 |
IN |
denotes |
in |
T2124 |
799-800 |
CD |
denotes |
5 |
T2125 |
801-803 |
NN |
denotes |
ml |
T2126 |
823-839 |
NN |
denotes |
paraformaldehyde |
T2127 |
804-812 |
JJ |
denotes |
fixative |
T2128 |
813-814 |
-LRB- |
denotes |
( |
T2129 |
814-815 |
CD |
denotes |
2 |
T2130 |
815-816 |
NN |
denotes |
% |
T2131 |
817-818 |
-LRB- |
denotes |
( |
T2132 |
818-819 |
NN |
denotes |
w |
T2133 |
819-820 |
SYM |
denotes |
/ |
T2134 |
820-821 |
NN |
denotes |
v |
T2135 |
821-822 |
-RRB- |
denotes |
) |
T2136 |
840-843 |
CC |
denotes |
and |
T2137 |
844-845 |
CD |
denotes |
2 |
T2138 |
845-846 |
NN |
denotes |
% |
T2139 |
847-861 |
NN |
denotes |
glutaraldehyde |
T2140 |
862-863 |
-LRB- |
denotes |
( |
T2141 |
863-864 |
NN |
denotes |
v |
T2142 |
864-865 |
SYM |
denotes |
/ |
T2143 |
865-866 |
NN |
denotes |
v |
T2144 |
866-867 |
-RRB- |
denotes |
) |
T2145 |
868-870 |
IN |
denotes |
in |
T2146 |
871-874 |
CD |
denotes |
0.1 |
T2147 |
875-876 |
NN |
denotes |
M |
T2148 |
895-901 |
NN |
denotes |
buffer |
T2149 |
877-883 |
NN |
denotes |
sodium |
T2150 |
884-894 |
NN |
denotes |
cacodylate |
T2151 |
901-903 |
, |
denotes |
, |
T2152 |
903-905 |
NN |
denotes |
pH |
T2153 |
906-909 |
CD |
denotes |
7.2 |
T2154 |
909-910 |
-RRB- |
denotes |
) |
T2155 |
910-911 |
. |
denotes |
. |
T2156 |
911-1006 |
sentence |
denotes |
Eyes were fixed for at least 24 hr at room temperature (RT) prior to dissection of the lenses. |
T2157 |
912-916 |
NNS |
denotes |
Eyes |
T2158 |
922-927 |
VBN |
denotes |
fixed |
T2159 |
917-921 |
VBD |
denotes |
were |
T2160 |
928-931 |
IN |
denotes |
for |
T2161 |
932-934 |
RB |
denotes |
at |
T2162 |
941-943 |
CD |
denotes |
24 |
T2163 |
935-940 |
RBS |
denotes |
least |
T2164 |
944-946 |
NNS |
denotes |
hr |
T2165 |
947-949 |
IN |
denotes |
at |
T2166 |
950-954 |
NN |
denotes |
room |
T2167 |
955-966 |
NN |
denotes |
temperature |
T2168 |
967-968 |
-LRB- |
denotes |
( |
T2169 |
968-970 |
NN |
denotes |
RT |
T2170 |
970-971 |
-RRB- |
denotes |
) |
T2171 |
972-977 |
JJ |
denotes |
prior |
T2172 |
978-980 |
IN |
denotes |
to |
T2173 |
981-991 |
NN |
denotes |
dissection |
T2174 |
992-994 |
IN |
denotes |
of |
T2175 |
995-998 |
DT |
denotes |
the |
T2176 |
999-1005 |
NNS |
denotes |
lenses |
T2177 |
1005-1006 |
. |
denotes |
. |
T2178 |
1006-1109 |
sentence |
denotes |
At this time, equatorial and axial dimensions of lenses and gross lenticular appearance were recorded. |
T2179 |
1007-1009 |
IN |
denotes |
At |
T2180 |
1100-1108 |
VBN |
denotes |
recorded |
T2181 |
1010-1014 |
DT |
denotes |
this |
T2182 |
1015-1019 |
NN |
denotes |
time |
T2183 |
1019-1021 |
, |
denotes |
, |
T2184 |
1021-1031 |
JJ |
denotes |
equatorial |
T2185 |
1042-1052 |
NNS |
denotes |
dimensions |
T2186 |
1032-1035 |
CC |
denotes |
and |
T2187 |
1036-1041 |
JJ |
denotes |
axial |
T2188 |
1053-1055 |
IN |
denotes |
of |
T2189 |
1056-1062 |
NNS |
denotes |
lenses |
T2190 |
1063-1066 |
CC |
denotes |
and |
T2191 |
1067-1072 |
JJ |
denotes |
gross |
T2192 |
1084-1094 |
NN |
denotes |
appearance |
T2193 |
1073-1083 |
JJ |
denotes |
lenticular |
T2194 |
1095-1099 |
VBD |
denotes |
were |
T2195 |
1108-1109 |
. |
denotes |
. |
T2196 |
1109-1290 |
sentence |
denotes |
Statistical analyses on data consisted of Student's t-test, means and standard deviation, using the statistical software package StatMost (Dataxiom Software Inc., Los Angeles, CA). |
T2197 |
1110-1121 |
JJ |
denotes |
Statistical |
T2198 |
1122-1130 |
NNS |
denotes |
analyses |
T2199 |
1139-1148 |
VBD |
denotes |
consisted |
T2200 |
1131-1133 |
IN |
denotes |
on |
T2201 |
1134-1138 |
NNS |
denotes |
data |
T2202 |
1149-1151 |
IN |
denotes |
of |
T2203 |
1152-1159 |
NN |
denotes |
Student |
T2204 |
1164-1168 |
NN |
denotes |
test |
T2205 |
1159-1161 |
POS |
denotes |
's |
T2206 |
1162-1163 |
NN |
denotes |
t |
T2207 |
1163-1164 |
HYPH |
denotes |
- |
T2208 |
1168-1170 |
, |
denotes |
, |
T2209 |
1170-1175 |
NN |
denotes |
means |
T2210 |
1176-1179 |
CC |
denotes |
and |
T2211 |
1180-1188 |
JJ |
denotes |
standard |
T2212 |
1189-1198 |
NN |
denotes |
deviation |
T2213 |
1198-1200 |
, |
denotes |
, |
T2214 |
1200-1205 |
VBG |
denotes |
using |
T2215 |
1206-1209 |
DT |
denotes |
the |
T2216 |
1231-1238 |
NN |
denotes |
package |
T2217 |
1210-1221 |
JJ |
denotes |
statistical |
T2218 |
1222-1230 |
NN |
denotes |
software |
T2219 |
1239-1247 |
NN |
denotes |
StatMost |
T2220 |
1248-1249 |
-LRB- |
denotes |
( |
T2221 |
1277-1284 |
NNP |
denotes |
Angeles |
T2222 |
1249-1257 |
NNP |
denotes |
Dataxiom |
T2223 |
1267-1271 |
NNP |
denotes |
Inc. |
T2224 |
1258-1266 |
NNP |
denotes |
Software |
T2225 |
1271-1273 |
, |
denotes |
, |
T2226 |
1273-1276 |
NNP |
denotes |
Los |
T2227 |
1284-1286 |
, |
denotes |
, |
T2228 |
1286-1288 |
NNP |
denotes |
CA |
T2229 |
1288-1289 |
-RRB- |
denotes |
) |
T2230 |
1289-1290 |
. |
denotes |
. |
T2289 |
1292-1300 |
JJ |
denotes |
Confocal |
T2290 |
1301-1311 |
NN |
denotes |
microscopy |
T2291 |
1311-1436 |
sentence |
denotes |
Lenses were vibratome sectioned into 100–200 μm thick sections along the optical (anterior to posterior) or equatorial axes. |
T2292 |
1312-1318 |
NNS |
denotes |
Lenses |
T2293 |
1334-1343 |
VBN |
denotes |
sectioned |
T2294 |
1319-1323 |
VBD |
denotes |
were |
T2295 |
1324-1333 |
NN |
denotes |
vibratome |
T2296 |
1344-1348 |
IN |
denotes |
into |
T2297 |
1349-1352 |
CD |
denotes |
100 |
T2298 |
1353-1356 |
CD |
denotes |
200 |
T2299 |
1352-1353 |
SYM |
denotes |
– |
T2300 |
1357-1359 |
NN |
denotes |
μm |
T2301 |
1360-1365 |
JJ |
denotes |
thick |
T2302 |
1366-1374 |
NNS |
denotes |
sections |
T2303 |
1375-1380 |
IN |
denotes |
along |
T2304 |
1381-1384 |
DT |
denotes |
the |
T2305 |
1431-1435 |
NNS |
denotes |
axes |
T2306 |
1385-1392 |
JJ |
denotes |
optical |
T2307 |
1393-1394 |
-LRB- |
denotes |
( |
T2308 |
1394-1402 |
JJ |
denotes |
anterior |
T2309 |
1403-1405 |
IN |
denotes |
to |
T2310 |
1406-1415 |
JJ |
denotes |
posterior |
T2311 |
1415-1416 |
-RRB- |
denotes |
) |
T2312 |
1417-1419 |
CC |
denotes |
or |
T2313 |
1420-1430 |
JJ |
denotes |
equatorial |
T2314 |
1435-1436 |
. |
denotes |
. |
T2315 |
1436-1662 |
sentence |
denotes |
Thick sections were fixed in 2% (w/v) paraformaldehyde and 2% glutaraldehyde (v/v) in 0.1 M sodium cacodylate buffer pH 7.2 at RT for 24 hrs then washed several times in Tris buffered saline (0.5 M Tris, 150 mM NaCl, pH 7.4). |
T2316 |
1437-1442 |
JJ |
denotes |
Thick |
T2317 |
1443-1451 |
NNS |
denotes |
sections |
T2318 |
1457-1462 |
VBN |
denotes |
fixed |
T2319 |
1452-1456 |
VBD |
denotes |
were |
T2320 |
1463-1465 |
IN |
denotes |
in |
T2321 |
1466-1467 |
CD |
denotes |
2 |
T2322 |
1467-1468 |
NN |
denotes |
% |
T2323 |
1475-1491 |
NN |
denotes |
paraformaldehyde |
T2324 |
1469-1470 |
-LRB- |
denotes |
( |
T2325 |
1470-1471 |
NN |
denotes |
w |
T2326 |
1471-1472 |
SYM |
denotes |
/ |
T2327 |
1472-1473 |
NN |
denotes |
v |
T2328 |
1473-1474 |
-RRB- |
denotes |
) |
T2329 |
1492-1495 |
CC |
denotes |
and |
T2330 |
1496-1497 |
CD |
denotes |
2 |
T2331 |
1497-1498 |
NN |
denotes |
% |
T2332 |
1499-1513 |
NN |
denotes |
glutaraldehyde |
T2333 |
1514-1515 |
-LRB- |
denotes |
( |
T2334 |
1515-1516 |
NN |
denotes |
v |
T2335 |
1516-1517 |
SYM |
denotes |
/ |
T2336 |
1517-1518 |
NN |
denotes |
v |
T2337 |
1518-1519 |
-RRB- |
denotes |
) |
T2338 |
1520-1522 |
IN |
denotes |
in |
T2339 |
1523-1526 |
CD |
denotes |
0.1 |
T2340 |
1527-1528 |
NN |
denotes |
M |
T2341 |
1547-1553 |
NN |
denotes |
buffer |
T2342 |
1529-1535 |
NN |
denotes |
sodium |
T2343 |
1536-1546 |
NN |
denotes |
cacodylate |
T2344 |
1554-1556 |
NN |
denotes |
pH |
T2345 |
1557-1560 |
CD |
denotes |
7.2 |
T2346 |
1561-1563 |
IN |
denotes |
at |
T2347 |
1564-1566 |
NN |
denotes |
RT |
T2348 |
1567-1570 |
IN |
denotes |
for |
T2349 |
1571-1573 |
CD |
denotes |
24 |
T2350 |
1574-1577 |
NNS |
denotes |
hrs |
T2351 |
1578-1582 |
RB |
denotes |
then |
T2352 |
1583-1589 |
VBD |
denotes |
washed |
T2353 |
1590-1597 |
JJ |
denotes |
several |
T2354 |
1598-1603 |
NNS |
denotes |
times |
T2355 |
1604-1606 |
IN |
denotes |
in |
T2356 |
1607-1611 |
NNP |
denotes |
Tris |
T2357 |
1612-1620 |
VBN |
denotes |
buffered |
T2358 |
1621-1627 |
NN |
denotes |
saline |
T2359 |
1628-1629 |
-LRB- |
denotes |
( |
T2360 |
1654-1656 |
NN |
denotes |
pH |
T2361 |
1629-1632 |
CD |
denotes |
0.5 |
T2362 |
1633-1634 |
NN |
denotes |
M |
T2363 |
1635-1639 |
NNP |
denotes |
Tris |
T2364 |
1639-1641 |
, |
denotes |
, |
T2365 |
1641-1644 |
CD |
denotes |
150 |
T2366 |
1645-1647 |
NN |
denotes |
mM |
T2367 |
1648-1652 |
NN |
denotes |
NaCl |
T2368 |
1652-1654 |
, |
denotes |
, |
T2369 |
1657-1660 |
CD |
denotes |
7.4 |
T2370 |
1660-1661 |
-RRB- |
denotes |
) |
T2371 |
1661-1662 |
. |
denotes |
. |
T2372 |
1662-1849 |
sentence |
denotes |
To visualize lipid membranes, sections were stained with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate ('DiI';DiIC18; Molecular Probes) as previously described [24]. |
T2373 |
1663-1665 |
TO |
denotes |
To |
T2374 |
1666-1675 |
VB |
denotes |
visualize |
T2375 |
1707-1714 |
VBN |
denotes |
stained |
T2376 |
1676-1681 |
NN |
denotes |
lipid |
T2377 |
1682-1691 |
NNS |
denotes |
membranes |
T2378 |
1691-1693 |
, |
denotes |
, |
T2379 |
1693-1701 |
NNS |
denotes |
sections |
T2380 |
1702-1706 |
VBD |
denotes |
were |
T2381 |
1715-1719 |
IN |
denotes |
with |
T2382 |
1720-1721 |
CD |
denotes |
1 |
T2383 |
1725-1736 |
NN |
denotes |
dioctadecyl |
T2384 |
1721-1722 |
, |
denotes |
, |
T2385 |
1722-1723 |
CD |
denotes |
1 |
T2386 |
1723-1724 |
SYM |
denotes |
' |
T2387 |
1724-1725 |
HYPH |
denotes |
- |
T2388 |
1736-1737 |
HYPH |
denotes |
- |
T2389 |
1737-1738 |
CD |
denotes |
3 |
T2390 |
1738-1739 |
, |
denotes |
, |
T2391 |
1739-1740 |
CD |
denotes |
3 |
T2392 |
1775-1786 |
NN |
denotes |
perchlorate |
T2393 |
1740-1741 |
, |
denotes |
, |
T2394 |
1741-1742 |
CD |
denotes |
3 |
T2395 |
1742-1743 |
SYM |
denotes |
' |
T2396 |
1743-1744 |
, |
denotes |
, |
T2397 |
1744-1745 |
CD |
denotes |
3 |
T2398 |
1745-1746 |
SYM |
denotes |
' |
T2399 |
1746-1747 |
HYPH |
denotes |
- |
T2400 |
1747-1774 |
JJ |
denotes |
tetramethylindocarbocyanine |
T2401 |
1787-1788 |
-LRB- |
denotes |
( |
T2402 |
1812-1818 |
NNP |
denotes |
Probes |
T2403 |
1788-1789 |
`` |
denotes |
' |
T2404 |
1789-1792 |
NN |
denotes |
DiI |
T2405 |
1792-1793 |
'' |
denotes |
' |
T2406 |
1793-1794 |
: |
denotes |
; |
T2407 |
1794-1800 |
NN |
denotes |
DiIC18 |
T2408 |
1800-1801 |
: |
denotes |
; |
T2409 |
1802-1811 |
NNP |
denotes |
Molecular |
T2410 |
1818-1819 |
-RRB- |
denotes |
) |
T2411 |
1820-1822 |
IN |
denotes |
as |
T2412 |
1834-1843 |
VBN |
denotes |
described |
T2413 |
1823-1833 |
RB |
denotes |
previously |
T2414 |
1844-1845 |
-LRB- |
denotes |
[ |
T2415 |
1845-1847 |
CD |
denotes |
24 |
T2416 |
1847-1848 |
-RRB- |
denotes |
] |
T2417 |
1848-1849 |
. |
denotes |
. |
T2418 |
1849-2104 |
sentence |
denotes |
Sections were then washed several times with Tris buffered saline, and nucleic acids were stained by incubating sections in TBS containing 1 μM SYTOX Green (Molecular Probes) for 10 min at RT followed by several washes with TBS prior to confocal imaging. |
T2419 |
1850-1858 |
NNS |
denotes |
Sections |
T2420 |
1869-1875 |
VBN |
denotes |
washed |
T2421 |
1859-1863 |
VBD |
denotes |
were |
T2422 |
1864-1868 |
RB |
denotes |
then |
T2423 |
1876-1883 |
JJ |
denotes |
several |
T2424 |
1884-1889 |
NNS |
denotes |
times |
T2425 |
1890-1894 |
IN |
denotes |
with |
T2426 |
1895-1899 |
NNP |
denotes |
Tris |
T2427 |
1900-1908 |
VBN |
denotes |
buffered |
T2428 |
1909-1915 |
NN |
denotes |
saline |
T2429 |
1915-1917 |
, |
denotes |
, |
T2430 |
1917-1920 |
CC |
denotes |
and |
T2431 |
1921-1928 |
JJ |
denotes |
nucleic |
T2432 |
1929-1934 |
NNS |
denotes |
acids |
T2433 |
1940-1947 |
VBN |
denotes |
stained |
T2434 |
1935-1939 |
VBD |
denotes |
were |
T2435 |
1948-1950 |
IN |
denotes |
by |
T2436 |
1951-1961 |
VBG |
denotes |
incubating |
T2437 |
1962-1970 |
NNS |
denotes |
sections |
T2438 |
1971-1973 |
IN |
denotes |
in |
T2439 |
1974-1977 |
NN |
denotes |
TBS |
T2440 |
1978-1988 |
VBG |
denotes |
containing |
T2441 |
1989-1990 |
CD |
denotes |
1 |
T2442 |
1991-1993 |
NN |
denotes |
μM |
T2443 |
2000-2005 |
NN |
denotes |
Green |
T2444 |
1994-1999 |
NN |
denotes |
SYTOX |
T2445 |
2006-2007 |
-LRB- |
denotes |
( |
T2446 |
2017-2023 |
NNP |
denotes |
Probes |
T2447 |
2007-2016 |
JJ |
denotes |
Molecular |
T2448 |
2023-2024 |
-RRB- |
denotes |
) |
T2449 |
2025-2028 |
IN |
denotes |
for |
T2450 |
2029-2031 |
CD |
denotes |
10 |
T2451 |
2032-2035 |
NN |
denotes |
min |
T2452 |
2036-2038 |
IN |
denotes |
at |
T2453 |
2039-2041 |
NN |
denotes |
RT |
T2454 |
2042-2050 |
VBN |
denotes |
followed |
T2455 |
2051-2053 |
IN |
denotes |
by |
T2456 |
2054-2061 |
JJ |
denotes |
several |
T2457 |
2062-2068 |
NNS |
denotes |
washes |
T2458 |
2069-2073 |
IN |
denotes |
with |
T2459 |
2074-2077 |
NN |
denotes |
TBS |
T2460 |
2078-2083 |
RB |
denotes |
prior |
T2461 |
2084-2086 |
IN |
denotes |
to |
T2462 |
2087-2095 |
JJ |
denotes |
confocal |
T2463 |
2096-2103 |
NN |
denotes |
imaging |
T2464 |
2103-2104 |
. |
denotes |
. |
T2465 |
2104-2507 |
sentence |
denotes |
Lens sections were viewed on a Zeiss laser scanning confocal microscope model LSM 410 equipped with an Axiovert 100 inverted microscope, an Argon-Krypton 488/568/647 laser, a KP line selection filter, a FT 488/568 Dichroic beam splitter, a FT 560 Dichroic beam splitter, a LP 590 emission filter for viewing DiI, a BP 515–540 emission filter for viewing SYTOX green, and the software package LSM 3.993. |
T2466 |
2105-2109 |
NNP |
denotes |
Lens |
T2467 |
2110-2118 |
NNS |
denotes |
sections |
T2468 |
2124-2130 |
VBN |
denotes |
viewed |
T2469 |
2119-2123 |
VBD |
denotes |
were |
T2470 |
2131-2133 |
IN |
denotes |
on |
T2471 |
2134-2135 |
DT |
denotes |
a |
T2472 |
2166-2176 |
NN |
denotes |
microscope |
T2473 |
2136-2141 |
NNP |
denotes |
Zeiss |
T2474 |
2142-2147 |
NN |
denotes |
laser |
T2475 |
2148-2156 |
NN |
denotes |
scanning |
T2476 |
2157-2165 |
JJ |
denotes |
confocal |
T2477 |
2177-2182 |
NN |
denotes |
model |
T2478 |
2183-2186 |
NN |
denotes |
LSM |
T2479 |
2187-2190 |
CD |
denotes |
410 |
T2480 |
2191-2199 |
VBN |
denotes |
equipped |
T2481 |
2200-2204 |
IN |
denotes |
with |
T2482 |
2205-2207 |
DT |
denotes |
an |
T2483 |
2230-2240 |
NN |
denotes |
microscope |
T2484 |
2208-2216 |
JJ |
denotes |
Axiovert |
T2485 |
2217-2220 |
CD |
denotes |
100 |
T2486 |
2221-2229 |
JJ |
denotes |
inverted |
T2487 |
2240-2242 |
, |
denotes |
, |
T2488 |
2242-2244 |
DT |
denotes |
an |
T2489 |
2271-2276 |
NN |
denotes |
laser |
T2490 |
2245-2250 |
JJ |
denotes |
Argon |
T2491 |
2251-2258 |
JJ |
denotes |
Krypton |
T2492 |
2250-2251 |
HYPH |
denotes |
- |
T2493 |
2259-2262 |
CD |
denotes |
488 |
T2494 |
2262-2263 |
HYPH |
denotes |
/ |
T2495 |
2263-2266 |
CD |
denotes |
568 |
T2496 |
2266-2267 |
HYPH |
denotes |
/ |
T2497 |
2267-2270 |
CD |
denotes |
647 |
T2498 |
2276-2278 |
, |
denotes |
, |
T2499 |
2278-2279 |
DT |
denotes |
a |
T2500 |
2298-2304 |
NN |
denotes |
filter |
T2501 |
2280-2282 |
NN |
denotes |
KP |
T2502 |
2283-2287 |
NN |
denotes |
line |
T2503 |
2288-2297 |
NN |
denotes |
selection |
T2504 |
2304-2306 |
, |
denotes |
, |
T2505 |
2306-2307 |
DT |
denotes |
a |
T2506 |
2333-2341 |
NN |
denotes |
splitter |
T2507 |
2308-2310 |
NN |
denotes |
FT |
T2508 |
2311-2314 |
CD |
denotes |
488 |
T2509 |
2314-2315 |
HYPH |
denotes |
/ |
T2510 |
2315-2318 |
CD |
denotes |
568 |
T2511 |
2319-2327 |
JJ |
denotes |
Dichroic |
T2512 |
2328-2332 |
NN |
denotes |
beam |
T2513 |
2341-2343 |
, |
denotes |
, |
T2514 |
2343-2344 |
DT |
denotes |
a |
T2515 |
2366-2374 |
NN |
denotes |
splitter |
T2516 |
2345-2347 |
NN |
denotes |
FT |
T2517 |
2348-2351 |
CD |
denotes |
560 |
T2518 |
2352-2360 |
JJ |
denotes |
Dichroic |
T2519 |
2361-2365 |
NN |
denotes |
beam |
T2520 |
2374-2376 |
, |
denotes |
, |
T2521 |
2376-2377 |
DT |
denotes |
a |
T2522 |
2394-2400 |
NN |
denotes |
filter |
T2523 |
2378-2380 |
NN |
denotes |
LP |
T2524 |
2381-2384 |
CD |
denotes |
590 |
T2525 |
2385-2393 |
NN |
denotes |
emission |
T2526 |
2401-2404 |
IN |
denotes |
for |
T2527 |
2405-2412 |
VBG |
denotes |
viewing |
T2528 |
2413-2416 |
NNP |
denotes |
DiI |
T2529 |
2416-2418 |
, |
denotes |
, |
T2530 |
2418-2419 |
DT |
denotes |
a |
T2531 |
2440-2446 |
NN |
denotes |
filter |
T2532 |
2420-2422 |
NN |
denotes |
BP |
T2533 |
2423-2426 |
CD |
denotes |
515 |
T2534 |
2426-2427 |
SYM |
denotes |
– |
T2535 |
2427-2430 |
CD |
denotes |
540 |
T2536 |
2431-2439 |
NN |
denotes |
emission |
T2537 |
2447-2450 |
IN |
denotes |
for |
T2538 |
2451-2458 |
VBG |
denotes |
viewing |
T2539 |
2459-2464 |
NN |
denotes |
SYTOX |
T2540 |
2465-2470 |
NN |
denotes |
green |
T2541 |
2470-2472 |
, |
denotes |
, |
T2542 |
2472-2475 |
CC |
denotes |
and |
T2543 |
2476-2479 |
DT |
denotes |
the |
T2544 |
2489-2496 |
NN |
denotes |
package |
T2545 |
2480-2488 |
NN |
denotes |
software |
T2546 |
2497-2500 |
NN |
denotes |
LSM |
T2547 |
2501-2506 |
CD |
denotes |
3.993 |
T2548 |
2506-2507 |
. |
denotes |
. |
T2588 |
2509-2517 |
NN |
denotes |
Scanning |
T2589 |
2527-2537 |
NN |
denotes |
microscopy |
T2590 |
2518-2526 |
NN |
denotes |
electron |
T2591 |
2537-2713 |
sentence |
denotes |
Lenses bisected along the optical axes were immersion fixed in 2% (w/v) paraformaldehyde and 2% glutaraldehyde (v/v) in 0.1 M sodium cacodylate buffer pH 7.2 at RT for 24 hrs. |
T2592 |
2538-2544 |
NNS |
denotes |
Lenses |
T2593 |
2592-2597 |
VBN |
denotes |
fixed |
T2594 |
2545-2553 |
VBN |
denotes |
bisected |
T2595 |
2554-2559 |
IN |
denotes |
along |
T2596 |
2560-2563 |
DT |
denotes |
the |
T2597 |
2572-2576 |
NNS |
denotes |
axes |
T2598 |
2564-2571 |
JJ |
denotes |
optical |
T2599 |
2577-2581 |
VBD |
denotes |
were |
T2600 |
2582-2591 |
NN |
denotes |
immersion |
T2601 |
2598-2600 |
IN |
denotes |
in |
T2602 |
2601-2602 |
CD |
denotes |
2 |
T2603 |
2602-2603 |
NN |
denotes |
% |
T2604 |
2610-2626 |
NN |
denotes |
paraformaldehyde |
T2605 |
2604-2605 |
-LRB- |
denotes |
( |
T2606 |
2605-2606 |
NN |
denotes |
w |
T2607 |
2606-2607 |
SYM |
denotes |
/ |
T2608 |
2607-2608 |
NN |
denotes |
v |
T2609 |
2608-2609 |
-RRB- |
denotes |
) |
T2610 |
2627-2630 |
CC |
denotes |
and |
T2611 |
2631-2632 |
CD |
denotes |
2 |
T2612 |
2632-2633 |
NN |
denotes |
% |
T2613 |
2634-2648 |
NN |
denotes |
glutaraldehyde |
T2614 |
2649-2650 |
-LRB- |
denotes |
( |
T2615 |
2650-2651 |
NN |
denotes |
v |
T2616 |
2651-2652 |
SYM |
denotes |
/ |
T2617 |
2652-2653 |
NN |
denotes |
v |
T2618 |
2653-2654 |
-RRB- |
denotes |
) |
T2619 |
2655-2657 |
IN |
denotes |
in |
T2620 |
2658-2661 |
CD |
denotes |
0.1 |
T2621 |
2662-2663 |
NN |
denotes |
M |
T2622 |
2671-2681 |
NN |
denotes |
cacodylate |
T2623 |
2664-2670 |
NN |
denotes |
sodium |
T2624 |
2682-2688 |
NN |
denotes |
buffer |
T2625 |
2689-2691 |
NN |
denotes |
pH |
T2626 |
2692-2695 |
CD |
denotes |
7.2 |
T2627 |
2696-2698 |
IN |
denotes |
at |
T2628 |
2699-2701 |
NN |
denotes |
RT |
T2629 |
2702-2705 |
IN |
denotes |
for |
T2630 |
2706-2708 |
CD |
denotes |
24 |
T2631 |
2709-2712 |
NNS |
denotes |
hrs |
T2632 |
2712-2713 |
. |
denotes |
. |
T2633 |
2713-2840 |
sentence |
denotes |
Samples were then washed several times with distilled water and postfixed in 1% (v/v) aqueous osmium tetroxide at RT for 1 hr. |
T2634 |
2714-2721 |
NNS |
denotes |
Samples |
T2635 |
2732-2738 |
VBN |
denotes |
washed |
T2636 |
2722-2726 |
VBD |
denotes |
were |
T2637 |
2727-2731 |
RB |
denotes |
then |
T2638 |
2739-2746 |
JJ |
denotes |
several |
T2639 |
2747-2752 |
NNS |
denotes |
times |
T2640 |
2753-2757 |
IN |
denotes |
with |
T2641 |
2758-2767 |
JJ |
denotes |
distilled |
T2642 |
2768-2773 |
NN |
denotes |
water |
T2643 |
2774-2777 |
CC |
denotes |
and |
T2644 |
2778-2787 |
VBN |
denotes |
postfixed |
T2645 |
2788-2790 |
IN |
denotes |
in |
T2646 |
2791-2792 |
CD |
denotes |
1 |
T2647 |
2792-2793 |
NN |
denotes |
% |
T2648 |
2815-2824 |
NN |
denotes |
tetroxide |
T2649 |
2794-2795 |
-LRB- |
denotes |
( |
T2650 |
2795-2796 |
NN |
denotes |
v |
T2651 |
2796-2797 |
SYM |
denotes |
/ |
T2652 |
2797-2798 |
NN |
denotes |
v |
T2653 |
2798-2799 |
-RRB- |
denotes |
) |
T2654 |
2800-2807 |
JJ |
denotes |
aqueous |
T2655 |
2808-2814 |
NN |
denotes |
osmium |
T2656 |
2825-2827 |
IN |
denotes |
at |
T2657 |
2828-2830 |
NN |
denotes |
RT |
T2658 |
2831-2834 |
IN |
denotes |
for |
T2659 |
2835-2836 |
CD |
denotes |
1 |
T2660 |
2837-2839 |
NN |
denotes |
hr |
T2661 |
2839-2840 |
. |
denotes |
. |
T2662 |
2840-2914 |
sentence |
denotes |
Lens halves were dehydrated in an ascending ethanol series from 50–100 %. |
T2663 |
2841-2845 |
NNP |
denotes |
Lens |
T2664 |
2846-2852 |
NNS |
denotes |
halves |
T2665 |
2858-2868 |
VBN |
denotes |
dehydrated |
T2666 |
2853-2857 |
VBD |
denotes |
were |
T2667 |
2869-2871 |
IN |
denotes |
in |
T2668 |
2872-2874 |
DT |
denotes |
an |
T2669 |
2893-2899 |
NN |
denotes |
series |
T2670 |
2875-2884 |
VBG |
denotes |
ascending |
T2671 |
2885-2892 |
NN |
denotes |
ethanol |
T2672 |
2900-2904 |
IN |
denotes |
from |
T2673 |
2905-2907 |
CD |
denotes |
50 |
T2674 |
2908-2911 |
CD |
denotes |
100 |
T2675 |
2907-2908 |
SYM |
denotes |
– |
T2676 |
2912-2913 |
NN |
denotes |
% |
T2677 |
2913-2914 |
. |
denotes |
. |
T2678 |
2914-3061 |
sentence |
denotes |
Once lens halves were in 100% ethanol, they were critical point dried in carbon dioxide in a Samdri-790B (Tousimis Research Corp., Rockville, MD). |
T2679 |
2915-2919 |
IN |
denotes |
Once |
T2680 |
2932-2936 |
VBD |
denotes |
were |
T2681 |
2920-2924 |
NN |
denotes |
lens |
T2682 |
2925-2931 |
NNS |
denotes |
halves |
T2683 |
2979-2984 |
VBN |
denotes |
dried |
T2684 |
2937-2939 |
IN |
denotes |
in |
T2685 |
2940-2943 |
CD |
denotes |
100 |
T2686 |
2943-2944 |
NN |
denotes |
% |
T2687 |
2945-2952 |
NN |
denotes |
ethanol |
T2688 |
2952-2954 |
, |
denotes |
, |
T2689 |
2954-2958 |
PRP |
denotes |
they |
T2690 |
2959-2963 |
VBD |
denotes |
were |
T2691 |
2964-2972 |
JJ |
denotes |
critical |
T2692 |
2973-2978 |
NN |
denotes |
point |
T2693 |
2985-2987 |
IN |
denotes |
in |
T2694 |
2988-2994 |
NN |
denotes |
carbon |
T2695 |
2995-3002 |
NN |
denotes |
dioxide |
T2696 |
3003-3005 |
IN |
denotes |
in |
T2697 |
3006-3007 |
DT |
denotes |
a |
T2698 |
3015-3019 |
NN |
denotes |
790B |
T2699 |
3008-3014 |
NN |
denotes |
Samdri |
T2700 |
3014-3015 |
HYPH |
denotes |
- |
T2701 |
3020-3021 |
-LRB- |
denotes |
( |
T2702 |
3039-3044 |
NNP |
denotes |
Corp. |
T2703 |
3021-3029 |
NNP |
denotes |
Tousimis |
T2704 |
3030-3038 |
NNP |
denotes |
Research |
T2705 |
3044-3046 |
, |
denotes |
, |
T2706 |
3046-3055 |
NNP |
denotes |
Rockville |
T2707 |
3055-3057 |
, |
denotes |
, |
T2708 |
3057-3059 |
NNP |
denotes |
MD |
T2709 |
3059-3060 |
-RRB- |
denotes |
) |
T2710 |
3060-3061 |
. |
denotes |
. |
T2711 |
3061-3149 |
sentence |
denotes |
Critical point dried lens halves were secured on aluminum stubs with double sided tape. |
T2712 |
3062-3070 |
JJ |
denotes |
Critical |
T2713 |
3071-3076 |
NN |
denotes |
point |
T2714 |
3077-3082 |
VBN |
denotes |
dried |
T2715 |
3088-3094 |
NNS |
denotes |
halves |
T2716 |
3083-3087 |
NN |
denotes |
lens |
T2717 |
3100-3107 |
VBN |
denotes |
secured |
T2718 |
3095-3099 |
VBD |
denotes |
were |
T2719 |
3108-3110 |
IN |
denotes |
on |
T2720 |
3111-3119 |
NN |
denotes |
aluminum |
T2721 |
3120-3125 |
NNS |
denotes |
stubs |
T2722 |
3126-3130 |
IN |
denotes |
with |
T2723 |
3131-3137 |
NN |
denotes |
double |
T2724 |
3138-3143 |
VBN |
denotes |
sided |
T2725 |
3144-3148 |
NN |
denotes |
tape |
T2726 |
3148-3149 |
. |
denotes |
. |
T2727 |
3149-3289 |
sentence |
denotes |
Mounted specimens were then sputter coated with gold and viewed on a Hitachi S-3500N scanning electron microscope (Tokyo, Japan) at 1–5 kV. |
T2728 |
3150-3157 |
VBN |
denotes |
Mounted |
T2729 |
3158-3167 |
NNS |
denotes |
specimens |
T2730 |
3186-3192 |
VBN |
denotes |
coated |
T2731 |
3168-3172 |
VBD |
denotes |
were |
T2732 |
3173-3177 |
RB |
denotes |
then |
T2733 |
3178-3185 |
NN |
denotes |
sputter |
T2734 |
3193-3197 |
IN |
denotes |
with |
T2735 |
3198-3202 |
NN |
denotes |
gold |
T2736 |
3203-3206 |
CC |
denotes |
and |
T2737 |
3207-3213 |
VBN |
denotes |
viewed |
T2738 |
3214-3216 |
IN |
denotes |
on |
T2739 |
3217-3218 |
DT |
denotes |
a |
T2740 |
3253-3263 |
NN |
denotes |
microscope |
T2741 |
3219-3226 |
NNP |
denotes |
Hitachi |
T2742 |
3229-3234 |
NN |
denotes |
3500N |
T2743 |
3227-3228 |
NN |
denotes |
S |
T2744 |
3228-3229 |
HYPH |
denotes |
- |
T2745 |
3235-3243 |
NN |
denotes |
scanning |
T2746 |
3244-3252 |
NN |
denotes |
electron |
T2747 |
3264-3265 |
-LRB- |
denotes |
( |
T2748 |
3265-3270 |
NNP |
denotes |
Tokyo |
T2749 |
3270-3272 |
, |
denotes |
, |
T2750 |
3272-3277 |
NNP |
denotes |
Japan |
T2751 |
3277-3278 |
-RRB- |
denotes |
) |
T2752 |
3279-3281 |
IN |
denotes |
at |
T2753 |
3282-3283 |
CD |
denotes |
1 |
T2754 |
3284-3285 |
CD |
denotes |
5 |
T2755 |
3283-3284 |
SYM |
denotes |
– |
T2756 |
3286-3288 |
NN |
denotes |
kV |
T2757 |
3288-3289 |
. |
denotes |
. |
T2758 |
3289-3443 |
sentence |
denotes |
This microscope is equipped with Hitachi's patented "Dual-Bias" which allows extremely high emission currents at acceleration voltages of 5 kV and lower. |
T2759 |
3290-3294 |
DT |
denotes |
This |
T2760 |
3295-3305 |
NN |
denotes |
microscope |
T2761 |
3309-3317 |
VBN |
denotes |
equipped |
T2762 |
3306-3308 |
VBZ |
denotes |
is |
T2763 |
3318-3322 |
IN |
denotes |
with |
T2764 |
3323-3330 |
NNP |
denotes |
Hitachi |
T2765 |
3348-3352 |
NNP |
denotes |
Bias |
T2766 |
3330-3332 |
POS |
denotes |
's |
T2767 |
3333-3341 |
VBN |
denotes |
patented |
T2768 |
3342-3343 |
`` |
denotes |
" |
T2769 |
3343-3347 |
NNP |
denotes |
Dual |
T2770 |
3347-3348 |
HYPH |
denotes |
- |
T2771 |
3352-3353 |
'' |
denotes |
" |
T2772 |
3354-3359 |
WDT |
denotes |
which |
T2773 |
3360-3366 |
VBZ |
denotes |
allows |
T2774 |
3367-3376 |
RB |
denotes |
extremely |
T2775 |
3377-3381 |
JJ |
denotes |
high |
T2776 |
3391-3399 |
NNS |
denotes |
currents |
T2777 |
3382-3390 |
NN |
denotes |
emission |
T2778 |
3400-3402 |
IN |
denotes |
at |
T2779 |
3403-3415 |
NN |
denotes |
acceleration |
T2780 |
3416-3424 |
NNS |
denotes |
voltages |
T2781 |
3425-3427 |
IN |
denotes |
of |
T2782 |
3428-3429 |
CD |
denotes |
5 |
T2783 |
3430-3432 |
NN |
denotes |
kV |
T2784 |
3433-3436 |
CC |
denotes |
and |
T2785 |
3437-3442 |
JJR |
denotes |
lower |
T2786 |
3442-3443 |
. |
denotes |
. |
R1281 |
T1953 |
T1954 |
det |
The,strain |
R1282 |
T1954 |
T1958 |
nsubj |
strain,was |
R1283 |
T1955 |
T1956 |
amod |
wild,type |
R1284 |
T1956 |
T1954 |
compound |
type,strain |
R1285 |
T1957 |
T1954 |
compound |
mouse,strain |
R1286 |
T1959 |
T1954 |
acl |
used,strain |
R1287 |
T1960 |
T1959 |
prep |
in,used |
R1288 |
T1961 |
T1962 |
det |
this,study |
R1289 |
T1962 |
T1960 |
pobj |
study,in |
R1290 |
T1963 |
T1964 |
det |
the,mouse |
R1291 |
T1964 |
T1958 |
attr |
mouse,was |
R1292 |
T1965 |
T1964 |
compound |
129SvEvTac,mouse |
R1293 |
T1966 |
T1958 |
punct |
.,was |
R1294 |
T1968 |
T1969 |
nsubj |
Lenses,were |
R1295 |
T1970 |
T1968 |
acl |
examined,Lenses |
R1296 |
T1971 |
T1969 |
prep |
from,were |
R1297 |
T1972 |
T1973 |
nummod |
5,wk |
R1298 |
T1973 |
T1990 |
npadvmod |
wk,old |
R1299 |
T1974 |
T1975 |
punct |
(,lenses |
R1300 |
T1975 |
T1972 |
parataxis |
lenses,5 |
R1301 |
T1976 |
T1975 |
nummod |
8,lenses |
R1302 |
T1977 |
T1975 |
punct |
),lenses |
R1303 |
T1978 |
T1972 |
punct |
", ",5 |
R1304 |
T1979 |
T1972 |
conj |
46,5 |
R1305 |
T1980 |
T1981 |
punct |
(,lenses |
R1306 |
T1981 |
T1979 |
parataxis |
lenses,46 |
R1307 |
T1982 |
T1981 |
nummod |
8,lenses |
R1308 |
T1983 |
T1981 |
punct |
),lenses |
R1309 |
T1984 |
T1979 |
cc |
and,46 |
R1310 |
T1985 |
T1979 |
conj |
72,46 |
R1311 |
T1986 |
T1987 |
punct |
(,lenses |
R1312 |
T1987 |
T1985 |
parataxis |
lenses,72 |
R1313 |
T1988 |
T1987 |
nummod |
6,lenses |
R1314 |
T1989 |
T1987 |
punct |
),lenses |
R1315 |
T1990 |
T1991 |
amod |
old,mice |
R1316 |
T1991 |
T1971 |
pobj |
mice,from |
R1317 |
T1992 |
T1969 |
punct |
.,were |
R1318 |
T1994 |
T1995 |
compound |
AlphaA,BKO |
R1319 |
T1995 |
T1997 |
nsubjpass |
BKO,generated |
R1320 |
T1996 |
T1995 |
punct |
/,BKO |
R1321 |
T1998 |
T1997 |
auxpass |
was,generated |
R1322 |
T1999 |
T1997 |
prep |
by,generated |
R1323 |
T2000 |
T2001 |
dep |
cross,breeding |
R1324 |
T2001 |
T1999 |
pcomp |
breeding,by |
R1325 |
T2002 |
T2003 |
nmod |
alphaAKO,127 |
R1326 |
T2003 |
T2005 |
nmod |
127,mice |
R1327 |
T2004 |
T2003 |
punct |
-,127 |
R1328 |
T2005 |
T2001 |
dobj |
mice,breeding |
R1329 |
T2006 |
T2007 |
punct |
[,22 |
R1330 |
T2007 |
T2003 |
parataxis |
22,127 |
R1331 |
T2008 |
T2007 |
punct |
],22 |
R1332 |
T2009 |
T2003 |
cc |
and,127 |
R1333 |
T2010 |
T2011 |
compound |
alphaBKO,168 |
R1334 |
T2011 |
T2003 |
conj |
168,127 |
R1335 |
T2012 |
T2011 |
punct |
-,168 |
R1336 |
T2013 |
T2014 |
punct |
[,23 |
R1337 |
T2014 |
T2005 |
parataxis |
23,mice |
R1338 |
T2015 |
T2014 |
punct |
],23 |
R1339 |
T2016 |
T2001 |
punct |
", ",breeding |
R1340 |
T2017 |
T2018 |
advmod |
also,in |
R1341 |
T2018 |
T2001 |
prep |
in,breeding |
R1342 |
T2019 |
T2020 |
det |
a,background |
R1343 |
T2020 |
T2018 |
pobj |
background,in |
R1344 |
T2021 |
T2020 |
compound |
129Sv,background |
R1345 |
T2022 |
T1997 |
punct |
.,generated |
R1346 |
T2024 |
T2025 |
det |
These,mice |
R1347 |
T2025 |
T2026 |
nsubj |
mice,lack |
R1348 |
T2027 |
T2026 |
advmod |
also,lack |
R1349 |
T2028 |
T2029 |
det |
the,product |
R1350 |
T2029 |
T2026 |
dobj |
product,lack |
R1351 |
T2030 |
T2029 |
compound |
HSPB2,product |
R1352 |
T2031 |
T2029 |
compound |
gene,product |
R1353 |
T2032 |
T2033 |
punct |
[,23 |
R1354 |
T2033 |
T2026 |
parataxis |
23,lack |
R1355 |
T2034 |
T2033 |
punct |
],23 |
R1356 |
T2035 |
T2026 |
punct |
.,lack |
R1357 |
T2037 |
T2038 |
nsubj |
Lenses,were |
R1358 |
T2039 |
T2037 |
acl |
examined,Lenses |
R1359 |
T2040 |
T2038 |
prep |
from,were |
R1360 |
T2041 |
T2042 |
nummod |
5,wk |
R1361 |
T2042 |
T2043 |
npadvmod |
wk,old |
R1362 |
T2043 |
T2055 |
amod |
old,mice |
R1363 |
T2044 |
T2045 |
punct |
(,lenses |
R1364 |
T2045 |
T2042 |
parataxis |
lenses,wk |
R1365 |
T2046 |
T2045 |
nummod |
6,lenses |
R1366 |
T2047 |
T2045 |
punct |
),lenses |
R1367 |
T2048 |
T2042 |
cc |
and,wk |
R1368 |
T2049 |
T2050 |
nummod |
54,wk |
R1369 |
T2050 |
T2042 |
conj |
wk,wk |
R1370 |
T2051 |
T2052 |
punct |
(,lenses |
R1371 |
T2052 |
T2050 |
parataxis |
lenses,wk |
R1372 |
T2053 |
T2052 |
nummod |
16,lenses |
R1373 |
T2054 |
T2052 |
punct |
),lenses |
R1374 |
T2055 |
T2040 |
pobj |
mice,from |
R1375 |
T2056 |
T2038 |
punct |
.,were |
R1376 |
T2058 |
T2059 |
det |
All,animals |
R1377 |
T2059 |
T2060 |
nsubjpass |
animals,treated |
R1378 |
T2061 |
T2060 |
auxpass |
were,treated |
R1379 |
T2062 |
T2060 |
prep |
in,treated |
R1380 |
T2063 |
T2062 |
pobj |
accordance,in |
R1381 |
T2064 |
T2063 |
prep |
with,accordance |
R1382 |
T2065 |
T2066 |
det |
the,Statement |
R1383 |
T2066 |
T2064 |
pobj |
Statement,with |
R1384 |
T2067 |
T2066 |
nmod |
Association,Statement |
R1385 |
T2068 |
T2067 |
prep |
for,Association |
R1386 |
T2069 |
T2068 |
pobj |
Research,for |
R1387 |
T2070 |
T2069 |
prep |
in,Research |
R1388 |
T2071 |
T2070 |
pobj |
Vision,in |
R1389 |
T2072 |
T2071 |
cc |
and,Vision |
R1390 |
T2073 |
T2071 |
conj |
Ophthalmology,Vision |
R1391 |
T2074 |
T2066 |
prep |
for,Statement |
R1392 |
T2075 |
T2076 |
det |
the,Use |
R1393 |
T2076 |
T2074 |
pobj |
Use,for |
R1394 |
T2077 |
T2076 |
prep |
of,Use |
R1395 |
T2078 |
T2077 |
pobj |
Animals,of |
R1396 |
T2079 |
T2076 |
prep |
in,Use |
R1397 |
T2080 |
T2081 |
nmod |
Ophthalmic,Research |
R1398 |
T2081 |
T2079 |
pobj |
Research,in |
R1399 |
T2082 |
T2080 |
cc |
and,Ophthalmic |
R1400 |
T2083 |
T2080 |
conj |
Vision,Ophthalmic |
R1401 |
T2084 |
T2060 |
punct |
.,treated |
R1402 |
T2086 |
T2087 |
advmod |
Immediately,after |
R1403 |
T2087 |
T2088 |
prep |
after,enucleated |
R1404 |
T2089 |
T2087 |
pcomp |
euthanizing,after |
R1405 |
T2090 |
T2089 |
dobj |
animals,euthanizing |
R1406 |
T2091 |
T2088 |
punct |
", ",enucleated |
R1407 |
T2092 |
T2088 |
nsubjpass |
eyes,enucleated |
R1408 |
T2093 |
T2088 |
auxpass |
were,enucleated |
R1409 |
T2094 |
T2088 |
punct |
.,enucleated |
R1410 |
T2096 |
T2097 |
det |
A,blade |
R1411 |
T2097 |
T2099 |
nsubjpass |
blade,used |
R1412 |
T2098 |
T2097 |
compound |
scalpel,blade |
R1413 |
T2100 |
T2099 |
auxpass |
was,used |
R1414 |
T2101 |
T2099 |
advmod |
then,used |
R1415 |
T2102 |
T2103 |
aux |
to,make |
R1416 |
T2103 |
T2099 |
advcl |
make,used |
R1417 |
T2104 |
T2105 |
det |
a,incision |
R1418 |
T2105 |
T2103 |
dobj |
incision,make |
R1419 |
T2106 |
T2105 |
amod |
small,incision |
R1420 |
T2107 |
T2105 |
prep |
into,incision |
R1421 |
T2108 |
T2109 |
det |
the,chamber |
R1422 |
T2109 |
T2107 |
pobj |
chamber,into |
R1423 |
T2110 |
T2109 |
amod |
anterior,chamber |
R1424 |
T2111 |
T2109 |
prep |
near,chamber |
R1425 |
T2112 |
T2113 |
det |
the,equator |
R1426 |
T2113 |
T2111 |
pobj |
equator,near |
R1427 |
T2114 |
T2099 |
cc |
and,used |
R1428 |
T2115 |
T2116 |
advmod |
then,immersed |
R1429 |
T2116 |
T2099 |
conj |
immersed,used |
R1430 |
T2117 |
T2118 |
det |
both,eyes |
R1431 |
T2118 |
T2116 |
nsubjpass |
eyes,immersed |
R1432 |
T2119 |
T2118 |
prep |
from,eyes |
R1433 |
T2120 |
T2121 |
amod |
individual,animals |
R1434 |
T2121 |
T2119 |
pobj |
animals,from |
R1435 |
T2122 |
T2116 |
auxpass |
were,immersed |
R1436 |
T2123 |
T2116 |
prep |
in,immersed |
R1437 |
T2124 |
T2125 |
nummod |
5,ml |
R1438 |
T2125 |
T2126 |
nmod |
ml,paraformaldehyde |
R1439 |
T2126 |
T2123 |
pobj |
paraformaldehyde,in |
R1440 |
T2127 |
T2125 |
amod |
fixative,ml |
R1441 |
T2128 |
T2125 |
punct |
(,ml |
R1442 |
T2129 |
T2130 |
nummod |
2,% |
R1443 |
T2130 |
T2125 |
appos |
%,ml |
R1444 |
T2131 |
T2132 |
punct |
(,w |
R1445 |
T2132 |
T2130 |
parataxis |
w,% |
R1446 |
T2133 |
T2134 |
punct |
/,v |
R1447 |
T2134 |
T2132 |
prep |
v,w |
R1448 |
T2135 |
T2126 |
punct |
),paraformaldehyde |
R1449 |
T2136 |
T2126 |
cc |
and,paraformaldehyde |
R1450 |
T2137 |
T2138 |
nummod |
2,% |
R1451 |
T2138 |
T2139 |
compound |
%,glutaraldehyde |
R1452 |
T2139 |
T2126 |
conj |
glutaraldehyde,paraformaldehyde |
R1453 |
T2140 |
T2141 |
punct |
(,v |
R1454 |
T2141 |
T2139 |
parataxis |
v,glutaraldehyde |
R1455 |
T2142 |
T2143 |
punct |
/,v |
R1456 |
T2143 |
T2141 |
prep |
v,v |
R1457 |
T2144 |
T2141 |
punct |
),v |
R1458 |
T2145 |
T2126 |
prep |
in,paraformaldehyde |
R1459 |
T2146 |
T2147 |
nummod |
0.1,M |
R1460 |
T2147 |
T2148 |
compound |
M,buffer |
R1461 |
T2148 |
T2145 |
pobj |
buffer,in |
R1462 |
T2149 |
T2150 |
compound |
sodium,cacodylate |
R1463 |
T2150 |
T2148 |
compound |
cacodylate,buffer |
R1464 |
T2151 |
T2126 |
punct |
", ",paraformaldehyde |
R1465 |
T2152 |
T2126 |
npadvmod |
pH,paraformaldehyde |
R1466 |
T2153 |
T2152 |
nummod |
7.2,pH |
R1467 |
T2154 |
T2116 |
punct |
),immersed |
R1468 |
T2155 |
T2116 |
punct |
.,immersed |
R1469 |
T2157 |
T2158 |
nsubjpass |
Eyes,fixed |
R1470 |
T2159 |
T2158 |
auxpass |
were,fixed |
R1471 |
T2160 |
T2158 |
prep |
for,fixed |
R1472 |
T2161 |
T2162 |
advmod |
at,24 |
R1473 |
T2162 |
T2164 |
nummod |
24,hr |
R1474 |
T2163 |
T2162 |
advmod |
least,24 |
R1475 |
T2164 |
T2160 |
pobj |
hr,for |
R1476 |
T2165 |
T2158 |
prep |
at,fixed |
R1477 |
T2166 |
T2167 |
compound |
room,temperature |
R1478 |
T2167 |
T2165 |
pobj |
temperature,at |
R1479 |
T2168 |
T2167 |
punct |
(,temperature |
R1480 |
T2169 |
T2167 |
appos |
RT,temperature |
R1481 |
T2170 |
T2158 |
punct |
),fixed |
R1482 |
T2171 |
T2172 |
amod |
prior,to |
R1483 |
T2172 |
T2158 |
prep |
to,fixed |
R1484 |
T2173 |
T2172 |
pobj |
dissection,to |
R1485 |
T2174 |
T2173 |
prep |
of,dissection |
R1486 |
T2175 |
T2176 |
det |
the,lenses |
R1487 |
T2176 |
T2174 |
pobj |
lenses,of |
R1488 |
T2177 |
T2158 |
punct |
.,fixed |
R1489 |
T2179 |
T2180 |
prep |
At,recorded |
R1490 |
T2181 |
T2182 |
det |
this,time |
R1491 |
T2182 |
T2179 |
pobj |
time,At |
R1492 |
T2183 |
T2180 |
punct |
", ",recorded |
R1493 |
T2184 |
T2185 |
amod |
equatorial,dimensions |
R1494 |
T2185 |
T2180 |
nsubjpass |
dimensions,recorded |
R1495 |
T2186 |
T2184 |
cc |
and,equatorial |
R1496 |
T2187 |
T2184 |
conj |
axial,equatorial |
R1497 |
T2188 |
T2185 |
prep |
of,dimensions |
R1498 |
T2189 |
T2188 |
pobj |
lenses,of |
R1499 |
T2190 |
T2185 |
cc |
and,dimensions |
R1500 |
T2191 |
T2192 |
amod |
gross,appearance |
R1501 |
T2192 |
T2185 |
conj |
appearance,dimensions |
R1502 |
T2193 |
T2192 |
amod |
lenticular,appearance |
R1503 |
T2194 |
T2180 |
auxpass |
were,recorded |
R1504 |
T2195 |
T2180 |
punct |
.,recorded |
R1505 |
T2197 |
T2198 |
amod |
Statistical,analyses |
R1506 |
T2198 |
T2199 |
nsubj |
analyses,consisted |
R1507 |
T2200 |
T2198 |
prep |
on,analyses |
R1508 |
T2201 |
T2200 |
pobj |
data,on |
R1509 |
T2202 |
T2199 |
prep |
of,consisted |
R1510 |
T2203 |
T2204 |
poss |
Student,test |
R1511 |
T2204 |
T2202 |
pobj |
test,of |
R1512 |
T2205 |
T2203 |
case |
's,Student |
R1513 |
T2206 |
T2204 |
compound |
t,test |
R1514 |
T2207 |
T2204 |
punct |
-,test |
R1515 |
T2208 |
T2204 |
punct |
", ",test |
R1516 |
T2209 |
T2204 |
conj |
means,test |
R1517 |
T2210 |
T2209 |
cc |
and,means |
R1518 |
T2211 |
T2212 |
amod |
standard,deviation |
R1519 |
T2212 |
T2209 |
conj |
deviation,means |
R1520 |
T2213 |
T2199 |
punct |
", ",consisted |
R1521 |
T2214 |
T2199 |
advcl |
using,consisted |
R1522 |
T2215 |
T2216 |
det |
the,package |
R1523 |
T2216 |
T2214 |
dobj |
package,using |
R1524 |
T2217 |
T2216 |
amod |
statistical,package |
R1525 |
T2218 |
T2216 |
compound |
software,package |
R1526 |
T2219 |
T2216 |
appos |
StatMost,package |
R1527 |
T2220 |
T2221 |
punct |
(,Angeles |
R1528 |
T2221 |
T2199 |
parataxis |
Angeles,consisted |
R1529 |
T2222 |
T2223 |
compound |
Dataxiom,Inc. |
R1530 |
T2223 |
T2221 |
dep |
Inc.,Angeles |
R1531 |
T2224 |
T2223 |
compound |
Software,Inc. |
R1532 |
T2225 |
T2221 |
punct |
", ",Angeles |
R1533 |
T2226 |
T2221 |
compound |
Los,Angeles |
R1534 |
T2227 |
T2221 |
punct |
", ",Angeles |
R1535 |
T2228 |
T2221 |
npadvmod |
CA,Angeles |
R1536 |
T2229 |
T2221 |
punct |
),Angeles |
R1537 |
T2230 |
T2199 |
punct |
.,consisted |
R1538 |
T2289 |
T2290 |
amod |
Confocal,microscopy |
R1539 |
T2292 |
T2293 |
nsubjpass |
Lenses,sectioned |
R1540 |
T2294 |
T2293 |
auxpass |
were,sectioned |
R1541 |
T2295 |
T2293 |
dep |
vibratome,sectioned |
R1542 |
T2296 |
T2293 |
prep |
into,sectioned |
R1543 |
T2297 |
T2298 |
quantmod |
100,200 |
R1544 |
T2298 |
T2300 |
nummod |
200,μm |
R1545 |
T2299 |
T2298 |
punct |
–,200 |
R1546 |
T2300 |
T2301 |
npadvmod |
μm,thick |
R1547 |
T2301 |
T2302 |
amod |
thick,sections |
R1548 |
T2302 |
T2296 |
pobj |
sections,into |
R1549 |
T2303 |
T2293 |
prep |
along,sectioned |
R1550 |
T2304 |
T2305 |
det |
the,axes |
R1551 |
T2305 |
T2303 |
pobj |
axes,along |
R1552 |
T2306 |
T2305 |
amod |
optical,axes |
R1553 |
T2307 |
T2308 |
punct |
(,anterior |
R1554 |
T2308 |
T2306 |
parataxis |
anterior,optical |
R1555 |
T2309 |
T2308 |
prep |
to,anterior |
R1556 |
T2310 |
T2309 |
amod |
posterior,to |
R1557 |
T2311 |
T2308 |
punct |
),anterior |
R1558 |
T2312 |
T2306 |
cc |
or,optical |
R1559 |
T2313 |
T2306 |
conj |
equatorial,optical |
R1560 |
T2314 |
T2293 |
punct |
.,sectioned |
R1561 |
T2316 |
T2317 |
amod |
Thick,sections |
R1562 |
T2317 |
T2318 |
nsubjpass |
sections,fixed |
R1563 |
T2319 |
T2318 |
auxpass |
were,fixed |
R1564 |
T2320 |
T2318 |
prep |
in,fixed |
R1565 |
T2321 |
T2322 |
nummod |
2,% |
R1566 |
T2322 |
T2323 |
nmod |
%,paraformaldehyde |
R1567 |
T2323 |
T2320 |
pobj |
paraformaldehyde,in |
R1568 |
T2324 |
T2325 |
punct |
(,w |
R1569 |
T2325 |
T2322 |
parataxis |
w,% |
R1570 |
T2326 |
T2327 |
punct |
/,v |
R1571 |
T2327 |
T2325 |
prep |
v,w |
R1572 |
T2328 |
T2325 |
punct |
),w |
R1573 |
T2329 |
T2323 |
cc |
and,paraformaldehyde |
R1574 |
T2330 |
T2331 |
nummod |
2,% |
R1575 |
T2331 |
T2332 |
compound |
%,glutaraldehyde |
R1576 |
T2332 |
T2323 |
conj |
glutaraldehyde,paraformaldehyde |
R1577 |
T2333 |
T2334 |
punct |
(,v |
R1578 |
T2334 |
T2332 |
parataxis |
v,glutaraldehyde |
R1579 |
T2335 |
T2336 |
punct |
/,v |
R1580 |
T2336 |
T2334 |
prep |
v,v |
R1581 |
T2337 |
T2334 |
punct |
),v |
R1582 |
T2338 |
T2323 |
prep |
in,paraformaldehyde |
R1583 |
T2339 |
T2340 |
nummod |
0.1,M |
R1584 |
T2340 |
T2341 |
compound |
M,buffer |
R1585 |
T2341 |
T2338 |
pobj |
buffer,in |
R1586 |
T2342 |
T2343 |
compound |
sodium,cacodylate |
R1587 |
T2343 |
T2341 |
compound |
cacodylate,buffer |
R1588 |
T2344 |
T2323 |
npadvmod |
pH,paraformaldehyde |
R1589 |
T2345 |
T2344 |
nummod |
7.2,pH |
R1590 |
T2346 |
T2318 |
prep |
at,fixed |
R1591 |
T2347 |
T2346 |
pobj |
RT,at |
R1592 |
T2348 |
T2318 |
prep |
for,fixed |
R1593 |
T2349 |
T2350 |
nummod |
24,hrs |
R1594 |
T2350 |
T2348 |
pobj |
hrs,for |
R1595 |
T2351 |
T2352 |
advmod |
then,washed |
R1596 |
T2352 |
T2318 |
dep |
washed,fixed |
R1597 |
T2353 |
T2354 |
amod |
several,times |
R1598 |
T2354 |
T2352 |
npadvmod |
times,washed |
R1599 |
T2355 |
T2352 |
prep |
in,washed |
R1600 |
T2356 |
T2357 |
npadvmod |
Tris,buffered |
R1601 |
T2357 |
T2358 |
amod |
buffered,saline |
R1602 |
T2358 |
T2355 |
pobj |
saline,in |
R1603 |
T2359 |
T2360 |
punct |
(,pH |
R1604 |
T2360 |
T2358 |
parataxis |
pH,saline |
R1605 |
T2361 |
T2362 |
nummod |
0.5,M |
R1606 |
T2362 |
T2363 |
compound |
M,Tris |
R1607 |
T2363 |
T2360 |
dep |
Tris,pH |
R1608 |
T2364 |
T2360 |
punct |
", ",pH |
R1609 |
T2365 |
T2366 |
nummod |
150,mM |
R1610 |
T2366 |
T2367 |
compound |
mM,NaCl |
R1611 |
T2367 |
T2360 |
dep |
NaCl,pH |
R1612 |
T2368 |
T2360 |
punct |
", ",pH |
R1613 |
T2369 |
T2360 |
nummod |
7.4,pH |
R1614 |
T2370 |
T2360 |
punct |
),pH |
R1615 |
T2371 |
T2318 |
punct |
.,fixed |
R1616 |
T2373 |
T2374 |
aux |
To,visualize |
R1617 |
T2374 |
T2375 |
advcl |
visualize,stained |
R1618 |
T2376 |
T2377 |
compound |
lipid,membranes |
R1619 |
T2377 |
T2374 |
dobj |
membranes,visualize |
R1620 |
T2378 |
T2375 |
punct |
", ",stained |
R1621 |
T2379 |
T2375 |
nsubjpass |
sections,stained |
R1622 |
T2380 |
T2375 |
auxpass |
were,stained |
R1623 |
T2381 |
T2375 |
prep |
with,stained |
R1624 |
T2382 |
T2383 |
nummod |
1,dioctadecyl |
R1625 |
T2383 |
T2381 |
pobj |
dioctadecyl,with |
R1626 |
T2384 |
T2382 |
punct |
",",1 |
R1627 |
T2385 |
T2382 |
appos |
1,1 |
R1628 |
T2386 |
T2385 |
punct |
',1 |
R1629 |
T2387 |
T2383 |
punct |
-,dioctadecyl |
R1630 |
T2388 |
T2383 |
punct |
-,dioctadecyl |
R1631 |
T2389 |
T2383 |
nummod |
3,dioctadecyl |
R1632 |
T2390 |
T2383 |
punct |
",",dioctadecyl |
R1633 |
T2391 |
T2392 |
nummod |
3,perchlorate |
R1634 |
T2392 |
T2383 |
appos |
perchlorate,dioctadecyl |
R1635 |
T2393 |
T2391 |
punct |
",",3 |
R1636 |
T2394 |
T2391 |
appos |
3,3 |
R1637 |
T2395 |
T2394 |
punct |
',3 |
R1638 |
T2396 |
T2391 |
punct |
",",3 |
R1639 |
T2397 |
T2391 |
appos |
3,3 |
R1640 |
T2398 |
T2397 |
punct |
',3 |
R1641 |
T2399 |
T2391 |
punct |
-,3 |
R1642 |
T2400 |
T2391 |
amod |
tetramethylindocarbocyanine,3 |
R1643 |
T2401 |
T2402 |
punct |
(,Probes |
R1644 |
T2402 |
T2383 |
parataxis |
Probes,dioctadecyl |
R1645 |
T2403 |
T2402 |
punct |
',Probes |
R1646 |
T2404 |
T2402 |
dep |
DiI,Probes |
R1647 |
T2405 |
T2402 |
punct |
',Probes |
R1648 |
T2406 |
T2402 |
punct |
;,Probes |
R1649 |
T2407 |
T2402 |
dep |
DiIC18,Probes |
R1650 |
T2408 |
T2402 |
punct |
;,Probes |
R1651 |
T2409 |
T2402 |
compound |
Molecular,Probes |
R1652 |
T2410 |
T2402 |
punct |
),Probes |
R1653 |
T2411 |
T2412 |
mark |
as,described |
R1654 |
T2412 |
T2375 |
advcl |
described,stained |
R1655 |
T2413 |
T2412 |
advmod |
previously,described |
R1656 |
T2414 |
T2415 |
punct |
[,24 |
R1657 |
T2415 |
T2375 |
parataxis |
24,stained |
R1658 |
T2416 |
T2415 |
punct |
],24 |
R1659 |
T2417 |
T2375 |
punct |
.,stained |
R1660 |
T2419 |
T2420 |
nsubjpass |
Sections,washed |
R1661 |
T2421 |
T2420 |
auxpass |
were,washed |
R1662 |
T2422 |
T2420 |
advmod |
then,washed |
R1663 |
T2423 |
T2424 |
amod |
several,times |
R1664 |
T2424 |
T2420 |
npadvmod |
times,washed |
R1665 |
T2425 |
T2420 |
prep |
with,washed |
R1666 |
T2426 |
T2427 |
npadvmod |
Tris,buffered |
R1667 |
T2427 |
T2428 |
amod |
buffered,saline |
R1668 |
T2428 |
T2425 |
pobj |
saline,with |
R1669 |
T2429 |
T2420 |
punct |
", ",washed |
R1670 |
T2430 |
T2420 |
cc |
and,washed |
R1671 |
T2431 |
T2432 |
amod |
nucleic,acids |
R1672 |
T2432 |
T2433 |
nsubjpass |
acids,stained |
R1673 |
T2433 |
T2420 |
conj |
stained,washed |
R1674 |
T2434 |
T2433 |
auxpass |
were,stained |
R1675 |
T2435 |
T2433 |
prep |
by,stained |
R1676 |
T2436 |
T2435 |
pcomp |
incubating,by |
R1677 |
T2437 |
T2436 |
dobj |
sections,incubating |
R1678 |
T2438 |
T2436 |
prep |
in,incubating |
R1679 |
T2439 |
T2438 |
pobj |
TBS,in |
R1680 |
T2440 |
T2439 |
acl |
containing,TBS |
R1681 |
T2441 |
T2442 |
nummod |
1,μM |
R1682 |
T2442 |
T2443 |
compound |
μM,Green |
R1683 |
T2443 |
T2440 |
dobj |
Green,containing |
R1684 |
T2444 |
T2443 |
compound |
SYTOX,Green |
R1685 |
T2445 |
T2446 |
punct |
(,Probes |
R1686 |
T2446 |
T2440 |
parataxis |
Probes,containing |
R1687 |
T2447 |
T2446 |
amod |
Molecular,Probes |
R1688 |
T2448 |
T2446 |
punct |
),Probes |
R1689 |
T2449 |
T2436 |
prep |
for,incubating |
R1690 |
T2450 |
T2451 |
nummod |
10,min |
R1691 |
T2451 |
T2449 |
pobj |
min,for |
R1692 |
T2452 |
T2436 |
prep |
at,incubating |
R1693 |
T2453 |
T2452 |
pobj |
RT,at |
R1694 |
T2454 |
T2436 |
advcl |
followed,incubating |
R1695 |
T2455 |
T2454 |
agent |
by,followed |
R1696 |
T2456 |
T2457 |
amod |
several,washes |
R1697 |
T2457 |
T2455 |
pobj |
washes,by |
R1698 |
T2458 |
T2457 |
prep |
with,washes |
R1699 |
T2459 |
T2458 |
pobj |
TBS,with |
R1700 |
T2460 |
T2457 |
advmod |
prior,washes |
R1701 |
T2461 |
T2460 |
prep |
to,prior |
R1702 |
T2462 |
T2463 |
amod |
confocal,imaging |
R1703 |
T2463 |
T2461 |
pobj |
imaging,to |
R1704 |
T2464 |
T2433 |
punct |
.,stained |
R1705 |
T2466 |
T2467 |
compound |
Lens,sections |
R1706 |
T2467 |
T2468 |
nsubjpass |
sections,viewed |
R1707 |
T2469 |
T2468 |
auxpass |
were,viewed |
R1708 |
T2470 |
T2468 |
prep |
on,viewed |
R1709 |
T2471 |
T2472 |
det |
a,microscope |
R1710 |
T2472 |
T2470 |
pobj |
microscope,on |
R1711 |
T2473 |
T2474 |
nmod |
Zeiss,laser |
R1712 |
T2474 |
T2472 |
nmod |
laser,microscope |
R1713 |
T2475 |
T2472 |
nmod |
scanning,microscope |
R1714 |
T2476 |
T2472 |
amod |
confocal,microscope |
R1715 |
T2477 |
T2478 |
compound |
model,LSM |
R1716 |
T2478 |
T2472 |
appos |
LSM,microscope |
R1717 |
T2479 |
T2478 |
nummod |
410,LSM |
R1718 |
T2480 |
T2472 |
acl |
equipped,microscope |
R1719 |
T2481 |
T2480 |
prep |
with,equipped |
R1720 |
T2482 |
T2483 |
det |
an,microscope |
R1721 |
T2483 |
T2481 |
pobj |
microscope,with |
R1722 |
T2484 |
T2485 |
amod |
Axiovert,100 |
R1723 |
T2485 |
T2483 |
nummod |
100,microscope |
R1724 |
T2486 |
T2483 |
amod |
inverted,microscope |
R1725 |
T2487 |
T2483 |
punct |
", ",microscope |
R1726 |
T2488 |
T2489 |
det |
an,laser |
R1727 |
T2489 |
T2483 |
conj |
laser,microscope |
R1728 |
T2490 |
T2491 |
amod |
Argon,Krypton |
R1729 |
T2491 |
T2489 |
nmod |
Krypton,laser |
R1730 |
T2492 |
T2491 |
punct |
-,Krypton |
R1731 |
T2493 |
T2491 |
nummod |
488,Krypton |
R1732 |
T2494 |
T2491 |
punct |
/,Krypton |
R1733 |
T2495 |
T2491 |
nummod |
568,Krypton |
R1734 |
T2496 |
T2491 |
punct |
/,Krypton |
R1735 |
T2497 |
T2491 |
nummod |
647,Krypton |
R1736 |
T2498 |
T2489 |
punct |
", ",laser |
R1737 |
T2499 |
T2500 |
det |
a,filter |
R1738 |
T2500 |
T2489 |
conj |
filter,laser |
R1739 |
T2501 |
T2500 |
compound |
KP,filter |
R1740 |
T2502 |
T2503 |
compound |
line,selection |
R1741 |
T2503 |
T2500 |
compound |
selection,filter |
R1742 |
T2504 |
T2500 |
punct |
", ",filter |
R1743 |
T2505 |
T2506 |
det |
a,splitter |
R1744 |
T2506 |
T2500 |
conj |
splitter,filter |
R1745 |
T2507 |
T2506 |
nmod |
FT,splitter |
R1746 |
T2508 |
T2507 |
nummod |
488,FT |
R1747 |
T2509 |
T2507 |
punct |
/,FT |
R1748 |
T2510 |
T2507 |
nummod |
568,FT |
R1749 |
T2511 |
T2506 |
amod |
Dichroic,splitter |
R1750 |
T2512 |
T2506 |
compound |
beam,splitter |
R1751 |
T2513 |
T2506 |
punct |
", ",splitter |
R1752 |
T2514 |
T2515 |
det |
a,splitter |
R1753 |
T2515 |
T2506 |
conj |
splitter,splitter |
R1754 |
T2516 |
T2515 |
nmod |
FT,splitter |
R1755 |
T2517 |
T2516 |
nummod |
560,FT |
R1756 |
T2518 |
T2515 |
amod |
Dichroic,splitter |
R1757 |
T2519 |
T2515 |
compound |
beam,splitter |
R1758 |
T2520 |
T2515 |
punct |
", ",splitter |
R1759 |
T2521 |
T2522 |
det |
a,filter |
R1760 |
T2522 |
T2515 |
conj |
filter,splitter |
R1761 |
T2523 |
T2522 |
nmod |
LP,filter |
R1762 |
T2524 |
T2523 |
nummod |
590,LP |
R1763 |
T2525 |
T2522 |
compound |
emission,filter |
R1764 |
T2526 |
T2522 |
prep |
for,filter |
R1765 |
T2527 |
T2526 |
pcomp |
viewing,for |
R1766 |
T2528 |
T2527 |
dobj |
DiI,viewing |
R1767 |
T2529 |
T2522 |
punct |
", ",filter |
R1768 |
T2530 |
T2531 |
det |
a,filter |
R1769 |
T2531 |
T2522 |
conj |
filter,filter |
R1770 |
T2532 |
T2531 |
nmod |
BP,filter |
R1771 |
T2533 |
T2532 |
nummod |
515,BP |
R1772 |
T2534 |
T2535 |
punct |
–,540 |
R1773 |
T2535 |
T2532 |
prep |
540,BP |
R1774 |
T2536 |
T2531 |
compound |
emission,filter |
R1775 |
T2537 |
T2531 |
prep |
for,filter |
R1776 |
T2538 |
T2537 |
pcomp |
viewing,for |
R1777 |
T2539 |
T2540 |
compound |
SYTOX,green |
R1778 |
T2540 |
T2538 |
dobj |
green,viewing |
R1779 |
T2541 |
T2531 |
punct |
", ",filter |
R1780 |
T2542 |
T2531 |
cc |
and,filter |
R1781 |
T2543 |
T2544 |
det |
the,package |
R1782 |
T2544 |
T2531 |
conj |
package,filter |
R1783 |
T2545 |
T2544 |
compound |
software,package |
R1784 |
T2546 |
T2544 |
appos |
LSM,package |
R1785 |
T2547 |
T2546 |
nummod |
3.993,LSM |
R1786 |
T2548 |
T2468 |
punct |
.,viewed |
R1787 |
T2588 |
T2589 |
compound |
Scanning,microscopy |
R1788 |
T2590 |
T2589 |
compound |
electron,microscopy |
R1789 |
T2592 |
T2593 |
nsubjpass |
Lenses,fixed |
R1790 |
T2594 |
T2592 |
acl |
bisected,Lenses |
R1791 |
T2595 |
T2594 |
prep |
along,bisected |
R1792 |
T2596 |
T2597 |
det |
the,axes |
R1793 |
T2597 |
T2595 |
pobj |
axes,along |
R1794 |
T2598 |
T2597 |
amod |
optical,axes |
R1795 |
T2599 |
T2593 |
auxpass |
were,fixed |
R1796 |
T2600 |
T2593 |
dep |
immersion,fixed |
R1797 |
T2601 |
T2593 |
prep |
in,fixed |
R1798 |
T2602 |
T2603 |
nummod |
2,% |
R1799 |
T2603 |
T2604 |
nmod |
%,paraformaldehyde |
R1800 |
T2604 |
T2601 |
pobj |
paraformaldehyde,in |
R1801 |
T2605 |
T2606 |
punct |
(,w |
R1802 |
T2606 |
T2603 |
parataxis |
w,% |
R1803 |
T2607 |
T2608 |
punct |
/,v |
R1804 |
T2608 |
T2606 |
prep |
v,w |
R1805 |
T2609 |
T2606 |
punct |
),w |
R1806 |
T2610 |
T2604 |
cc |
and,paraformaldehyde |
R1807 |
T2611 |
T2612 |
nummod |
2,% |
R1808 |
T2612 |
T2613 |
compound |
%,glutaraldehyde |
R1809 |
T2613 |
T2604 |
conj |
glutaraldehyde,paraformaldehyde |
R1810 |
T2614 |
T2615 |
punct |
(,v |
R1811 |
T2615 |
T2613 |
parataxis |
v,glutaraldehyde |
R1812 |
T2616 |
T2617 |
punct |
/,v |
R1813 |
T2617 |
T2615 |
prep |
v,v |
R1814 |
T2618 |
T2615 |
punct |
),v |
R1815 |
T2619 |
T2604 |
prep |
in,paraformaldehyde |
R1816 |
T2620 |
T2621 |
nummod |
0.1,M |
R1817 |
T2621 |
T2622 |
compound |
M,cacodylate |
R1818 |
T2622 |
T2624 |
compound |
cacodylate,buffer |
R1819 |
T2623 |
T2622 |
compound |
sodium,cacodylate |
R1820 |
T2624 |
T2619 |
pobj |
buffer,in |
R1821 |
T2625 |
T2624 |
npadvmod |
pH,buffer |
R1822 |
T2626 |
T2625 |
nummod |
7.2,pH |
R1823 |
T2627 |
T2593 |
prep |
at,fixed |
R1824 |
T2628 |
T2627 |
pobj |
RT,at |
R1825 |
T2629 |
T2593 |
prep |
for,fixed |
R1826 |
T2630 |
T2631 |
nummod |
24,hrs |
R1827 |
T2631 |
T2629 |
pobj |
hrs,for |
R1828 |
T2632 |
T2593 |
punct |
.,fixed |
R1829 |
T2634 |
T2635 |
nsubjpass |
Samples,washed |
R1830 |
T2636 |
T2635 |
auxpass |
were,washed |
R1831 |
T2637 |
T2635 |
advmod |
then,washed |
R1832 |
T2638 |
T2639 |
amod |
several,times |
R1833 |
T2639 |
T2635 |
npadvmod |
times,washed |
R1834 |
T2640 |
T2635 |
prep |
with,washed |
R1835 |
T2641 |
T2642 |
amod |
distilled,water |
R1836 |
T2642 |
T2640 |
pobj |
water,with |
R1837 |
T2643 |
T2635 |
cc |
and,washed |
R1838 |
T2644 |
T2635 |
conj |
postfixed,washed |
R1839 |
T2645 |
T2644 |
prep |
in,postfixed |
R1840 |
T2646 |
T2647 |
nummod |
1,% |
R1841 |
T2647 |
T2648 |
nmod |
%,tetroxide |
R1842 |
T2648 |
T2645 |
pobj |
tetroxide,in |
R1843 |
T2649 |
T2650 |
punct |
(,v |
R1844 |
T2650 |
T2648 |
parataxis |
v,tetroxide |
R1845 |
T2651 |
T2652 |
punct |
/,v |
R1846 |
T2652 |
T2650 |
prep |
v,v |
R1847 |
T2653 |
T2650 |
punct |
),v |
R1848 |
T2654 |
T2648 |
amod |
aqueous,tetroxide |
R1849 |
T2655 |
T2648 |
compound |
osmium,tetroxide |
R1850 |
T2656 |
T2644 |
prep |
at,postfixed |
R1851 |
T2657 |
T2656 |
pobj |
RT,at |
R1852 |
T2658 |
T2644 |
prep |
for,postfixed |
R1853 |
T2659 |
T2660 |
nummod |
1,hr |
R1854 |
T2660 |
T2658 |
pobj |
hr,for |
R1855 |
T2661 |
T2635 |
punct |
.,washed |
R1856 |
T2663 |
T2664 |
compound |
Lens,halves |
R1857 |
T2664 |
T2665 |
nsubjpass |
halves,dehydrated |
R1858 |
T2666 |
T2665 |
auxpass |
were,dehydrated |
R1859 |
T2667 |
T2665 |
prep |
in,dehydrated |
R1860 |
T2668 |
T2669 |
det |
an,series |
R1861 |
T2669 |
T2667 |
pobj |
series,in |
R1862 |
T2670 |
T2669 |
amod |
ascending,series |
R1863 |
T2671 |
T2669 |
compound |
ethanol,series |
R1864 |
T2672 |
T2669 |
prep |
from,series |
R1865 |
T2673 |
T2674 |
quantmod |
50,100 |
R1866 |
T2674 |
T2676 |
nummod |
100,% |
R1867 |
T2675 |
T2674 |
punct |
–,100 |
R1868 |
T2676 |
T2672 |
pobj |
%,from |
R1869 |
T2677 |
T2665 |
punct |
.,dehydrated |
R1870 |
T2679 |
T2680 |
mark |
Once,were |
R1871 |
T2680 |
T2683 |
advcl |
were,dried |
R1872 |
T2681 |
T2682 |
compound |
lens,halves |
R1873 |
T2682 |
T2680 |
nsubj |
halves,were |
R1874 |
T2684 |
T2680 |
prep |
in,were |
R1875 |
T2685 |
T2686 |
nummod |
100,% |
R1876 |
T2686 |
T2687 |
compound |
%,ethanol |
R1877 |
T2687 |
T2684 |
pobj |
ethanol,in |
R1878 |
T2688 |
T2683 |
punct |
", ",dried |
R1879 |
T2689 |
T2683 |
nsubjpass |
they,dried |
R1880 |
T2690 |
T2683 |
auxpass |
were,dried |
R1881 |
T2691 |
T2692 |
amod |
critical,point |
R1882 |
T2692 |
T2683 |
npadvmod |
point,dried |
R1883 |
T2693 |
T2683 |
prep |
in,dried |
R1884 |
T2694 |
T2695 |
compound |
carbon,dioxide |
R1885 |
T2695 |
T2693 |
pobj |
dioxide,in |
R1886 |
T2696 |
T2683 |
prep |
in,dried |
R1887 |
T2697 |
T2698 |
det |
a,790B |
R1888 |
T2698 |
T2696 |
pobj |
790B,in |
R1889 |
T2699 |
T2698 |
compound |
Samdri,790B |
R1890 |
T2700 |
T2698 |
punct |
-,790B |
R1891 |
T2701 |
T2702 |
punct |
(,Corp. |
R1892 |
T2702 |
T2698 |
parataxis |
Corp.,790B |
R1893 |
T2703 |
T2702 |
compound |
Tousimis,Corp. |
R1894 |
T2704 |
T2702 |
compound |
Research,Corp. |
R1895 |
T2705 |
T2702 |
punct |
", ",Corp. |
R1896 |
T2706 |
T2702 |
npadvmod |
Rockville,Corp. |
R1897 |
T2707 |
T2702 |
punct |
", ",Corp. |
R1898 |
T2708 |
T2702 |
npadvmod |
MD,Corp. |
R1899 |
T2709 |
T2702 |
punct |
),Corp. |
R1900 |
T2710 |
T2683 |
punct |
.,dried |
R1901 |
T2712 |
T2713 |
amod |
Critical,point |
R1902 |
T2713 |
T2714 |
npadvmod |
point,dried |
R1903 |
T2714 |
T2715 |
amod |
dried,halves |
R1904 |
T2715 |
T2717 |
nsubjpass |
halves,secured |
R1905 |
T2716 |
T2715 |
compound |
lens,halves |
R1906 |
T2718 |
T2717 |
auxpass |
were,secured |
R1907 |
T2719 |
T2717 |
prep |
on,secured |
R1908 |
T2720 |
T2721 |
compound |
aluminum,stubs |
R1909 |
T2721 |
T2719 |
pobj |
stubs,on |
R1910 |
T2722 |
T2717 |
prep |
with,secured |
R1911 |
T2723 |
T2724 |
npadvmod |
double,sided |
R1912 |
T2724 |
T2725 |
amod |
sided,tape |
R1913 |
T2725 |
T2722 |
pobj |
tape,with |
R1914 |
T2726 |
T2717 |
punct |
.,secured |
R1915 |
T2728 |
T2729 |
amod |
Mounted,specimens |
R1916 |
T2729 |
T2730 |
nsubjpass |
specimens,coated |
R1917 |
T2731 |
T2730 |
auxpass |
were,coated |
R1918 |
T2732 |
T2730 |
advmod |
then,coated |
R1919 |
T2733 |
T2730 |
dep |
sputter,coated |
R1920 |
T2734 |
T2730 |
prep |
with,coated |
R1921 |
T2735 |
T2734 |
pobj |
gold,with |
R1922 |
T2736 |
T2730 |
cc |
and,coated |
R1923 |
T2737 |
T2730 |
conj |
viewed,coated |
R1924 |
T2738 |
T2737 |
prep |
on,viewed |
R1925 |
T2739 |
T2740 |
det |
a,microscope |
R1926 |
T2740 |
T2738 |
pobj |
microscope,on |
R1927 |
T2741 |
T2742 |
compound |
Hitachi,3500N |
R1928 |
T2742 |
T2740 |
compound |
3500N,microscope |
R1929 |
T2743 |
T2742 |
compound |
S,3500N |
R1930 |
T2744 |
T2742 |
punct |
-,3500N |
R1931 |
T2745 |
T2740 |
compound |
scanning,microscope |
R1932 |
T2746 |
T2740 |
compound |
electron,microscope |
R1933 |
T2747 |
T2748 |
punct |
(,Tokyo |
R1934 |
T2748 |
T2740 |
parataxis |
Tokyo,microscope |
R1935 |
T2749 |
T2748 |
punct |
", ",Tokyo |
R1936 |
T2750 |
T2748 |
npadvmod |
Japan,Tokyo |
R1937 |
T2751 |
T2748 |
punct |
),Tokyo |
R1938 |
T2752 |
T2737 |
prep |
at,viewed |
R1939 |
T2753 |
T2754 |
quantmod |
1,5 |
R1940 |
T2754 |
T2756 |
nummod |
5,kV |
R1941 |
T2755 |
T2754 |
punct |
–,5 |
R1942 |
T2756 |
T2752 |
pobj |
kV,at |
R1943 |
T2757 |
T2730 |
punct |
.,coated |
R1944 |
T2759 |
T2760 |
det |
This,microscope |
R1945 |
T2760 |
T2761 |
nsubjpass |
microscope,equipped |
R1946 |
T2762 |
T2761 |
auxpass |
is,equipped |
R1947 |
T2763 |
T2761 |
prep |
with,equipped |
R1948 |
T2764 |
T2765 |
poss |
Hitachi,Bias |
R1949 |
T2765 |
T2763 |
pobj |
Bias,with |
R1950 |
T2766 |
T2764 |
case |
's,Hitachi |
R1951 |
T2767 |
T2765 |
amod |
patented,Bias |
R1952 |
T2768 |
T2765 |
punct |
"""",Bias |
R1953 |
T2769 |
T2765 |
compound |
Dual,Bias |
R1954 |
T2770 |
T2765 |
punct |
-,Bias |
R1955 |
T2771 |
T2765 |
punct |
"""",Bias |
R1956 |
T2772 |
T2773 |
dep |
which,allows |
R1957 |
T2773 |
T2765 |
relcl |
allows,Bias |
R1958 |
T2774 |
T2775 |
advmod |
extremely,high |
R1959 |
T2775 |
T2776 |
amod |
high,currents |
R1960 |
T2776 |
T2773 |
dobj |
currents,allows |
R1961 |
T2777 |
T2776 |
compound |
emission,currents |
R1962 |
T2778 |
T2773 |
prep |
at,allows |
R1963 |
T2779 |
T2780 |
compound |
acceleration,voltages |
R1964 |
T2780 |
T2778 |
pobj |
voltages,at |
R1965 |
T2781 |
T2780 |
prep |
of,voltages |
R1966 |
T2782 |
T2783 |
nummod |
5,kV |
R1967 |
T2783 |
T2781 |
pobj |
kV,of |
R1968 |
T2784 |
T2783 |
cc |
and,kV |
R1969 |
T2785 |
T2783 |
conj |
lower,kV |
R1970 |
T2786 |
T2761 |
punct |
.,equipped |