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data were collected on approximately 40 mice (20 of each sex) from each congenic and control strain. To eliminate parity and reduce litter size effects only progeny from uniparous dams were characterized and all litters were standardized to 5–7 pups/litter. Mice were weaned at 3 weeks of age. Feed (Purina 5008; 23.5% protein, 6.5% fat, 3.3 Kcal/g) and water were offered ad libitum. Mice were weighed to the nearest 0.1 g at 2WK, 3WK, 6WK, and 9WK of age. At 9WK ( ± 5 days) mice were anesthetized under isoflurane and nasal-anal length (NA) and nasal-tail (NT) were measured to the nearest mm. Tail length was calculated as NA minus NT. Anesthetized mice were then sacrificed by decapitation and exsanguinated. Femoral fat pad (FFP), gonadal fat pad (GFP), mesenteric fat pad (MFP) and retroperitoneal fat pad (RFP) were removed and weighed to the nearest mg."}
craft-ca-core-ex-dev
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craft-ca-core-dev
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