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characterization\nTrait data were collected on approximately 40 mice (20 of each sex) from each congenic and control strain. To eliminate parity and reduce litter size effects only progeny from uniparous dams were characterized and all litters were standardized to 5–7 pups/litter. Mice were weaned at 3 weeks of age. Feed (Purina 5008; 23.5% protein, 6.5% fat, 3.3 Kcal/g) and water were offered ad libitum. Mice were weighed to the nearest 0.1 g at 2WK, 3WK, 6WK, and 9WK of age. At 9WK ( ± 5 days) mice were anesthetized under isoflurane and nasal-anal length (NA) and nasal-tail (NT) were measured to the nearest mm. Tail length was calculated as NA minus NT. Anesthetized mice were then sacrificed by decapitation and exsanguinated. Femoral fat pad (FFP), gonadal fat pad (GFP), mesenteric fat pad (MFP) and retroperitoneal fat pad (RFP) were removed and weighed to the nearest mg.\nChemical compositional analysis was performed for HGC, HG11 and HG17 carcasses as previously described with slight modifications [24]. Briefly, after weighing each fat pad was returned to the carcass. The entire gastrointestinal (GI) tract was subsequently removed and carcasses were again weighed. This represented the empty carcass weight (ECW). Carcasses were labeled and secured in two layers of cheesecloth (Fisher) and frozen at -20°C until analysis. At this time, carcasses were freeze-dried for seven days and water content was determined by subtracting the freeze-dried weight from ECW. FAT was then extracted with ether for 7 days, followed by acetone for an additional 7 days in a Soxhlet apparatus. Carcass ash (ASH) was determined measuring the remains after a 16 hour incineration at 575°C. Carcass protein (PROT) was calculated as the remaining portion after carcass fat (FAT) and ASH were subtracted from ECW."}

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The entire gastrointestinal (GI) tract was subsequently removed and carcasses were again weighed. This represented the empty carcass weight (ECW). Carcasses were labeled and secured in two layers of cheesecloth (Fisher) and frozen at -20°C until analysis. At this time, carcasses were freeze-dried for seven days and water content was determined by subtracting the freeze-dried weight from ECW. FAT was then extracted with ether for 7 days, followed by acetone for an additional 7 days in a Soxhlet apparatus. Carcass ash (ASH) was determined measuring the remains after a 16 hour incineration at 575°C. Carcass protein (PROT) was calculated as the remaining portion after carcass fat (FAT) and ASH were subtracted from ECW."}

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