PMC:1472690 / 38801-40018 JSON TXT

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pmc-enju-pas

bionlp-st-ge-2016-test-proteins

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T20293","span":{"begin":1131,"end":1137},"obj":"Protein"},{"id":"T20292","span":{"begin":881,"end":886},"obj":"Protein"},{"id":"T20291","span":{"begin":870,"end":876},"obj":"Protein"},{"id":"T20290","span":{"begin":688,"end":694},"obj":"Protein"},{"id":"T20289","span":{"begin":619,"end":620},"obj":"Protein"},{"id":"T20288","span":{"begin":612,"end":618},"obj":"Protein"},{"id":"T20287","span":{"begin":559,"end":565},"obj":"Protein"},{"id":"T20286","span":{"begin":69,"end":75},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"To inhibit translocation and binding of transcription factors to the IL-1RA promoter, PBMC were incubated for 1 h with each of the following substances (concentrations were based upon 50% inhibition): 50 μg/ml of CAPE (caffeic-3,4-dihydroxycinnamic-acid-phenyl-ester), (Biomol, Hamburg, Germany); 400 ng/ml cyclosporin A (Calbiochem-Merck Biosciences, Bad Soden, Germany); 10 μg/ml cycloheximide (Santa Cruz Biotechnology) prior to incubation with GP. Band shifts were determined after 1 h incubation with GP as described before, including mutated oligos for NFIL-6 (TTA CAA CAG TGG ATT GCG ACA CTT AGT GGG) and NFATP2/3 (GGC GCA GAA AAG GTA AAA TAT TTA CTA TCT) binding sites within the IL-1RA promoter. PBMC RNA was isolated with an RNeasy kit (Qiagen, Germany) and messenger RNA was transcribed into cDNA with Reactin Ready First Strand kit (Biomol) and analysed for IL-1RA and GAPDH transcripts by PCR with a HotStart \"sweet\" PCR mastermix (Biomol) (95°C, 30 sec, 55°C, 30 sec, 72°C, 30 sec, 25 cycles for IL-1RA and 94°C for 30 sec, 50°C for 30 sec and 72°C for 45 sec, 25 cycles for GAPDH) following 18 h incubation with GP. IL-1RA protein was measured in the supernatant by ELISA after 24 h incubation with GP."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T20582","span":{"begin":1089,"end":1094},"obj":"http://www.uniprot.org/uniprot/P04406"},{"id":"T20581","span":{"begin":881,"end":886},"obj":"http://www.uniprot.org/uniprot/P04406"},{"id":"T20580","span":{"begin":1131,"end":1137},"obj":"http://www.uniprot.org/uniprot/P18510"},{"id":"T20579","span":{"begin":1010,"end":1016},"obj":"http://www.uniprot.org/uniprot/P18510"},{"id":"T20578","span":{"begin":870,"end":876},"obj":"http://www.uniprot.org/uniprot/P18510"},{"id":"T20577","span":{"begin":688,"end":694},"obj":"http://www.uniprot.org/uniprot/P18510"},{"id":"T20576","span":{"begin":69,"end":75},"obj":"http://www.uniprot.org/uniprot/P18510"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"To inhibit translocation and binding of transcription factors to the IL-1RA promoter, PBMC were incubated for 1 h with each of the following substances (concentrations were based upon 50% inhibition): 50 μg/ml of CAPE (caffeic-3,4-dihydroxycinnamic-acid-phenyl-ester), (Biomol, Hamburg, Germany); 400 ng/ml cyclosporin A (Calbiochem-Merck Biosciences, Bad Soden, Germany); 10 μg/ml cycloheximide (Santa Cruz Biotechnology) prior to incubation with GP. Band shifts were determined after 1 h incubation with GP as described before, including mutated oligos for NFIL-6 (TTA CAA CAG TGG ATT GCG ACA CTT AGT GGG) and NFATP2/3 (GGC GCA GAA AAG GTA AAA TAT TTA CTA TCT) binding sites within the IL-1RA promoter. PBMC RNA was isolated with an RNeasy kit (Qiagen, Germany) and messenger RNA was transcribed into cDNA with Reactin Ready First Strand kit (Biomol) and analysed for IL-1RA and GAPDH transcripts by PCR with a HotStart \"sweet\" PCR mastermix (Biomol) (95°C, 30 sec, 55°C, 30 sec, 72°C, 30 sec, 25 cycles for IL-1RA and 94°C for 30 sec, 50°C for 30 sec and 72°C for 45 sec, 25 cycles for GAPDH) following 18 h incubation with GP. IL-1RA protein was measured in the supernatant by ELISA after 24 h incubation with GP."}

GO-BP

{"project":"GO-BP","denotations":[{"id":"T20272","span":{"begin":626,"end":629},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T20271","span":{"begin":40,"end":53},"obj":"http://purl.obolibrary.org/obo/GO_0006351"}],"text":"To inhibit translocation and binding of transcription factors to the IL-1RA promoter, PBMC were incubated for 1 h with each of the following substances (concentrations were based upon 50% inhibition): 50 μg/ml of CAPE (caffeic-3,4-dihydroxycinnamic-acid-phenyl-ester), (Biomol, Hamburg, Germany); 400 ng/ml cyclosporin A (Calbiochem-Merck Biosciences, Bad Soden, Germany); 10 μg/ml cycloheximide (Santa Cruz Biotechnology) prior to incubation with GP. Band shifts were determined after 1 h incubation with GP as described before, including mutated oligos for NFIL-6 (TTA CAA CAG TGG ATT GCG ACA CTT AGT GGG) and NFATP2/3 (GGC GCA GAA AAG GTA AAA TAT TTA CTA TCT) binding sites within the IL-1RA promoter. PBMC RNA was isolated with an RNeasy kit (Qiagen, Germany) and messenger RNA was transcribed into cDNA with Reactin Ready First Strand kit (Biomol) and analysed for IL-1RA and GAPDH transcripts by PCR with a HotStart \"sweet\" PCR mastermix (Biomol) (95°C, 30 sec, 55°C, 30 sec, 72°C, 30 sec, 25 cycles for IL-1RA and 94°C for 30 sec, 50°C for 30 sec and 72°C for 45 sec, 25 cycles for GAPDH) following 18 h incubation with GP. IL-1RA protein was measured in the supernatant by ELISA after 24 h incubation with GP."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T20300","span":{"begin":626,"end":629},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T20299","span":{"begin":29,"end":61},"obj":"http://purl.obolibrary.org/obo/GO_0070491"},{"id":"T20298","span":{"begin":29,"end":61},"obj":"http://purl.obolibrary.org/obo/GO_0043425"},{"id":"T20297","span":{"begin":29,"end":61},"obj":"http://purl.obolibrary.org/obo/GO_0033613"},{"id":"T20296","span":{"begin":29,"end":61},"obj":"http://purl.obolibrary.org/obo/GO_0008134"},{"id":"T20295","span":{"begin":663,"end":670},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T20294","span":{"begin":29,"end":36},"obj":"http://purl.obolibrary.org/obo/GO_0005488"}],"text":"To inhibit translocation and binding of transcription factors to the IL-1RA promoter, PBMC were incubated for 1 h with each of the following substances (concentrations were based upon 50% inhibition): 50 μg/ml of CAPE (caffeic-3,4-dihydroxycinnamic-acid-phenyl-ester), (Biomol, Hamburg, Germany); 400 ng/ml cyclosporin A (Calbiochem-Merck Biosciences, Bad Soden, Germany); 10 μg/ml cycloheximide (Santa Cruz Biotechnology) prior to incubation with GP. Band shifts were determined after 1 h incubation with GP as described before, including mutated oligos for NFIL-6 (TTA CAA CAG TGG ATT GCG ACA CTT AGT GGG) and NFATP2/3 (GGC GCA GAA AAG GTA AAA TAT TTA CTA TCT) binding sites within the IL-1RA promoter. PBMC RNA was isolated with an RNeasy kit (Qiagen, Germany) and messenger RNA was transcribed into cDNA with Reactin Ready First Strand kit (Biomol) and analysed for IL-1RA and GAPDH transcripts by PCR with a HotStart \"sweet\" PCR mastermix (Biomol) (95°C, 30 sec, 55°C, 30 sec, 72°C, 30 sec, 25 cycles for IL-1RA and 94°C for 30 sec, 50°C for 30 sec and 72°C for 45 sec, 25 cycles for GAPDH) following 18 h incubation with GP. IL-1RA protein was measured in the supernatant by ELISA after 24 h incubation with GP."}

sentences

{"project":"sentences","denotations":[{"id":"T20266","span":{"begin":1131,"end":1217},"obj":"Sentence"},{"id":"T20265","span":{"begin":705,"end":1130},"obj":"Sentence"},{"id":"T20264","span":{"begin":452,"end":704},"obj":"Sentence"},{"id":"T20263","span":{"begin":201,"end":451},"obj":"Sentence"},{"id":"T20262","span":{"begin":0,"end":200},"obj":"Sentence"},{"id":"T266","span":{"begin":0,"end":200},"obj":"Sentence"},{"id":"T267","span":{"begin":201,"end":451},"obj":"Sentence"},{"id":"T268","span":{"begin":452,"end":704},"obj":"Sentence"},{"id":"T269","span":{"begin":705,"end":1130},"obj":"Sentence"},{"id":"T270","span":{"begin":1131,"end":1217},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"To inhibit translocation and binding of transcription factors to the IL-1RA promoter, PBMC were incubated for 1 h with each of the following substances (concentrations were based upon 50% inhibition): 50 μg/ml of CAPE (caffeic-3,4-dihydroxycinnamic-acid-phenyl-ester), (Biomol, Hamburg, Germany); 400 ng/ml cyclosporin A (Calbiochem-Merck Biosciences, Bad Soden, Germany); 10 μg/ml cycloheximide (Santa Cruz Biotechnology) prior to incubation with GP. Band shifts were determined after 1 h incubation with GP as described before, including mutated oligos for NFIL-6 (TTA CAA CAG TGG ATT GCG ACA CTT AGT GGG) and NFATP2/3 (GGC GCA GAA AAG GTA AAA TAT TTA CTA TCT) binding sites within the IL-1RA promoter. PBMC RNA was isolated with an RNeasy kit (Qiagen, Germany) and messenger RNA was transcribed into cDNA with Reactin Ready First Strand kit (Biomol) and analysed for IL-1RA and GAPDH transcripts by PCR with a HotStart \"sweet\" PCR mastermix (Biomol) (95°C, 30 sec, 55°C, 30 sec, 72°C, 30 sec, 25 cycles for IL-1RA and 94°C for 30 sec, 50°C for 30 sec and 72°C for 45 sec, 25 cycles for GAPDH) following 18 h incubation with GP. IL-1RA protein was measured in the supernatant by ELISA after 24 h incubation with GP."}

simple1

{"project":"simple1","denotations":[{"id":"T20331","span":{"begin":1131,"end":1137},"obj":"Protein"},{"id":"T20330","span":{"begin":881,"end":886},"obj":"Protein"},{"id":"T20329","span":{"begin":870,"end":876},"obj":"Protein"},{"id":"T20328","span":{"begin":688,"end":694},"obj":"Protein"},{"id":"T20327","span":{"begin":619,"end":620},"obj":"Protein"},{"id":"T20326","span":{"begin":612,"end":618},"obj":"Protein"},{"id":"T20325","span":{"begin":559,"end":565},"obj":"Protein"},{"id":"T20324","span":{"begin":69,"end":75},"obj":"Protein"}],"text":"To inhibit translocation and binding of transcription factors to the IL-1RA promoter, PBMC were incubated for 1 h with each of the following substances (concentrations were based upon 50% inhibition): 50 μg/ml of CAPE (caffeic-3,4-dihydroxycinnamic-acid-phenyl-ester), (Biomol, Hamburg, Germany); 400 ng/ml cyclosporin A (Calbiochem-Merck Biosciences, Bad Soden, Germany); 10 μg/ml cycloheximide (Santa Cruz Biotechnology) prior to incubation with GP. Band shifts were determined after 1 h incubation with GP as described before, including mutated oligos for NFIL-6 (TTA CAA CAG TGG ATT GCG ACA CTT AGT GGG) and NFATP2/3 (GGC GCA GAA AAG GTA AAA TAT TTA CTA TCT) binding sites within the IL-1RA promoter. PBMC RNA was isolated with an RNeasy kit (Qiagen, Germany) and messenger RNA was transcribed into cDNA with Reactin Ready First Strand kit (Biomol) and analysed for IL-1RA and GAPDH transcripts by PCR with a HotStart \"sweet\" PCR mastermix (Biomol) (95°C, 30 sec, 55°C, 30 sec, 72°C, 30 sec, 25 cycles for IL-1RA and 94°C for 30 sec, 50°C for 30 sec and 72°C for 45 sec, 25 cycles for GAPDH) following 18 h incubation with GP. IL-1RA protein was measured in the supernatant by ELISA after 24 h incubation with GP."}

BioNLP16_DUT

{"project":"BioNLP16_DUT","denotations":[{"id":"T20956","span":{"begin":29,"end":36},"obj":"Binding"},{"id":"T20955","span":{"begin":3,"end":10},"obj":"Negative_regulation"},{"id":"T20954","span":{"begin":1131,"end":1137},"obj":"Protein"},{"id":"T20953","span":{"begin":881,"end":886},"obj":"Protein"},{"id":"T20952","span":{"begin":870,"end":876},"obj":"Protein"},{"id":"T20951","span":{"begin":688,"end":694},"obj":"Protein"},{"id":"T20950","span":{"begin":619,"end":620},"obj":"Protein"},{"id":"T20949","span":{"begin":612,"end":618},"obj":"Protein"},{"id":"T20948","span":{"begin":559,"end":565},"obj":"Protein"},{"id":"T20947","span":{"begin":69,"end":75},"obj":"Protein"}],"relations":[{"id":"R14428","pred":"themeOf","subj":"T20947","obj":"T20956"},{"id":"R14429","pred":"themeOf","subj":"T20956","obj":"T20955"}],"text":"To inhibit translocation and binding of transcription factors to the IL-1RA promoter, PBMC were incubated for 1 h with each of the following substances (concentrations were based upon 50% inhibition): 50 μg/ml of CAPE (caffeic-3,4-dihydroxycinnamic-acid-phenyl-ester), (Biomol, Hamburg, Germany); 400 ng/ml cyclosporin A (Calbiochem-Merck Biosciences, Bad Soden, Germany); 10 μg/ml cycloheximide (Santa Cruz Biotechnology) prior to incubation with GP. Band shifts were determined after 1 h incubation with GP as described before, including mutated oligos for NFIL-6 (TTA CAA CAG TGG ATT GCG ACA CTT AGT GGG) and NFATP2/3 (GGC GCA GAA AAG GTA AAA TAT TTA CTA TCT) binding sites within the IL-1RA promoter. PBMC RNA was isolated with an RNeasy kit (Qiagen, Germany) and messenger RNA was transcribed into cDNA with Reactin Ready First Strand kit (Biomol) and analysed for IL-1RA and GAPDH transcripts by PCR with a HotStart \"sweet\" PCR mastermix (Biomol) (95°C, 30 sec, 55°C, 30 sec, 72°C, 30 sec, 25 cycles for IL-1RA and 94°C for 30 sec, 50°C for 30 sec and 72°C for 45 sec, 25 cycles for GAPDH) following 18 h incubation with GP. IL-1RA protein was measured in the supernatant by ELISA after 24 h incubation with GP."}

BioNLP16_Messiy

{"project":"BioNLP16_Messiy","denotations":[{"id":"T20591","span":{"begin":29,"end":36},"obj":"Binding"},{"id":"T20590","span":{"begin":1131,"end":1137},"obj":"Protein"},{"id":"T20589","span":{"begin":881,"end":886},"obj":"Protein"},{"id":"T20588","span":{"begin":870,"end":876},"obj":"Protein"},{"id":"T20587","span":{"begin":688,"end":694},"obj":"Protein"},{"id":"T20586","span":{"begin":619,"end":620},"obj":"Protein"},{"id":"T20585","span":{"begin":612,"end":618},"obj":"Protein"},{"id":"T20584","span":{"begin":559,"end":565},"obj":"Protein"},{"id":"T20583","span":{"begin":69,"end":75},"obj":"Protein"}],"relations":[{"id":"R14139","pred":"themeOf","subj":"T20583","obj":"T20591"}],"text":"To inhibit translocation and binding of transcription factors to the IL-1RA promoter, PBMC were incubated for 1 h with each of the following substances (concentrations were based upon 50% inhibition): 50 μg/ml of CAPE (caffeic-3,4-dihydroxycinnamic-acid-phenyl-ester), (Biomol, Hamburg, Germany); 400 ng/ml cyclosporin A (Calbiochem-Merck Biosciences, Bad Soden, Germany); 10 μg/ml cycloheximide (Santa Cruz Biotechnology) prior to incubation with GP. Band shifts were determined after 1 h incubation with GP as described before, including mutated oligos for NFIL-6 (TTA CAA CAG TGG ATT GCG ACA CTT AGT GGG) and NFATP2/3 (GGC GCA GAA AAG GTA AAA TAT TTA CTA TCT) binding sites within the IL-1RA promoter. PBMC RNA was isolated with an RNeasy kit (Qiagen, Germany) and messenger RNA was transcribed into cDNA with Reactin Ready First Strand kit (Biomol) and analysed for IL-1RA and GAPDH transcripts by PCR with a HotStart \"sweet\" PCR mastermix (Biomol) (95°C, 30 sec, 55°C, 30 sec, 72°C, 30 sec, 25 cycles for IL-1RA and 94°C for 30 sec, 50°C for 30 sec and 72°C for 45 sec, 25 cycles for GAPDH) following 18 h incubation with GP. IL-1RA protein was measured in the supernatant by ELISA after 24 h incubation with GP."}

DLUT931

{"project":"DLUT931","denotations":[{"id":"T20601","span":{"begin":663,"end":670},"obj":"Binding"},{"id":"T20600","span":{"begin":29,"end":36},"obj":"Binding"},{"id":"T20599","span":{"begin":1131,"end":1137},"obj":"Protein"},{"id":"T20598","span":{"begin":881,"end":886},"obj":"Protein"},{"id":"T20597","span":{"begin":870,"end":876},"obj":"Protein"},{"id":"T20596","span":{"begin":688,"end":694},"obj":"Protein"},{"id":"T20595","span":{"begin":619,"end":620},"obj":"Protein"},{"id":"T20594","span":{"begin":612,"end":618},"obj":"Protein"},{"id":"T20593","span":{"begin":559,"end":565},"obj":"Protein"},{"id":"T20592","span":{"begin":69,"end":75},"obj":"Protein"}],"relations":[{"id":"R14140","pred":"themeOf","subj":"T20592","obj":"T20600"},{"id":"R14141","pred":"themeOf","subj":"T20593","obj":"T20601"},{"id":"R14142","pred":"themeOf","subj":"T20593","obj":"T20601"},{"id":"R14143","pred":"themeOf","subj":"T20594","obj":"T20601"},{"id":"R14144","pred":"themeOf","subj":"T20594","obj":"T20601"},{"id":"R14145","pred":"themeOf","subj":"T20595","obj":"T20601"},{"id":"R14146","pred":"themeOf","subj":"T20596","obj":"T20601"},{"id":"R14147","pred":"themeOf","subj":"T20596","obj":"T20601"},{"id":"R14148","pred":"themeOf","subj":"T20596","obj":"T20601"}],"text":"To inhibit translocation and binding of transcription factors to the IL-1RA promoter, PBMC were incubated for 1 h with each of the following substances (concentrations were based upon 50% inhibition): 50 μg/ml of CAPE (caffeic-3,4-dihydroxycinnamic-acid-phenyl-ester), (Biomol, Hamburg, Germany); 400 ng/ml cyclosporin A (Calbiochem-Merck Biosciences, Bad Soden, Germany); 10 μg/ml cycloheximide (Santa Cruz Biotechnology) prior to incubation with GP. Band shifts were determined after 1 h incubation with GP as described before, including mutated oligos for NFIL-6 (TTA CAA CAG TGG ATT GCG ACA CTT AGT GGG) and NFATP2/3 (GGC GCA GAA AAG GTA AAA TAT TTA CTA TCT) binding sites within the IL-1RA promoter. PBMC RNA was isolated with an RNeasy kit (Qiagen, Germany) and messenger RNA was transcribed into cDNA with Reactin Ready First Strand kit (Biomol) and analysed for IL-1RA and GAPDH transcripts by PCR with a HotStart \"sweet\" PCR mastermix (Biomol) (95°C, 30 sec, 55°C, 30 sec, 72°C, 30 sec, 25 cycles for IL-1RA and 94°C for 30 sec, 50°C for 30 sec and 72°C for 45 sec, 25 cycles for GAPDH) following 18 h incubation with GP. IL-1RA protein was measured in the supernatant by ELISA after 24 h incubation with GP."}

bionlp-st-ge-2016-test-ihmc

bionlp-st-ge-2016-spacy-parsed

bionlp-st-ge-2016-test-tees

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T20315","span":{"begin":1131,"end":1145},"obj":"Protein"},{"id":"T20314","span":{"begin":1089,"end":1094},"obj":"Protein"},{"id":"T20313","span":{"begin":1010,"end":1016},"obj":"Protein"},{"id":"T20312","span":{"begin":881,"end":898},"obj":"Protein"},{"id":"T20311","span":{"begin":870,"end":876},"obj":"Protein"},{"id":"T20310","span":{"begin":813,"end":820},"obj":"Protein"},{"id":"T20309","span":{"begin":688,"end":703},"obj":"Protein"},{"id":"T20308","span":{"begin":654,"end":661},"obj":"Protein"},{"id":"T20307","span":{"begin":634,"end":653},"obj":"Protein"},{"id":"T20306","span":{"begin":622,"end":629},"obj":"Protein"},{"id":"T20305","span":{"begin":612,"end":620},"obj":"Protein"},{"id":"T20304","span":{"begin":599,"end":606},"obj":"Protein"},{"id":"T20303","span":{"begin":583,"end":598},"obj":"Protein"},{"id":"T20302","span":{"begin":567,"end":582},"obj":"Protein"},{"id":"T20301","span":{"begin":559,"end":565},"obj":"Protein"}],"text":"To inhibit translocation and binding of transcription factors to the IL-1RA promoter, PBMC were incubated for 1 h with each of the following substances (concentrations were based upon 50% inhibition): 50 μg/ml of CAPE (caffeic-3,4-dihydroxycinnamic-acid-phenyl-ester), (Biomol, Hamburg, Germany); 400 ng/ml cyclosporin A (Calbiochem-Merck Biosciences, Bad Soden, Germany); 10 μg/ml cycloheximide (Santa Cruz Biotechnology) prior to incubation with GP. Band shifts were determined after 1 h incubation with GP as described before, including mutated oligos for NFIL-6 (TTA CAA CAG TGG ATT GCG ACA CTT AGT GGG) and NFATP2/3 (GGC GCA GAA AAG GTA AAA TAT TTA CTA TCT) binding sites within the IL-1RA promoter. PBMC RNA was isolated with an RNeasy kit (Qiagen, Germany) and messenger RNA was transcribed into cDNA with Reactin Ready First Strand kit (Biomol) and analysed for IL-1RA and GAPDH transcripts by PCR with a HotStart \"sweet\" PCR mastermix (Biomol) (95°C, 30 sec, 55°C, 30 sec, 72°C, 30 sec, 25 cycles for IL-1RA and 94°C for 30 sec, 50°C for 30 sec and 72°C for 45 sec, 25 cycles for GAPDH) following 18 h incubation with GP. IL-1RA protein was measured in the supernatant by ELISA after 24 h incubation with GP."}

testone

{"project":"testone","denotations":[{"id":"T20241","span":{"begin":76,"end":84},"obj":"Entity"},{"id":"T20240","span":{"begin":29,"end":36},"obj":"Binding"},{"id":"T20239","span":{"begin":1131,"end":1137},"obj":"Protein"},{"id":"T20238","span":{"begin":881,"end":886},"obj":"Protein"},{"id":"T20237","span":{"begin":870,"end":876},"obj":"Protein"},{"id":"T20236","span":{"begin":688,"end":694},"obj":"Protein"},{"id":"T20235","span":{"begin":619,"end":620},"obj":"Protein"},{"id":"T20234","span":{"begin":612,"end":618},"obj":"Protein"},{"id":"T20233","span":{"begin":559,"end":565},"obj":"Protein"},{"id":"T20232","span":{"begin":69,"end":75},"obj":"Protein"}],"relations":[{"id":"R13881","pred":"partOf","subj":"T20241","obj":"T20232"},{"id":"R13882","pred":"themeOf","subj":"T20241","obj":"T20240"}],"text":"To inhibit translocation and binding of transcription factors to the IL-1RA promoter, PBMC were incubated for 1 h with each of the following substances (concentrations were based upon 50% inhibition): 50 μg/ml of CAPE (caffeic-3,4-dihydroxycinnamic-acid-phenyl-ester), (Biomol, Hamburg, Germany); 400 ng/ml cyclosporin A (Calbiochem-Merck Biosciences, Bad Soden, Germany); 10 μg/ml cycloheximide (Santa Cruz Biotechnology) prior to incubation with GP. Band shifts were determined after 1 h incubation with GP as described before, including mutated oligos for NFIL-6 (TTA CAA CAG TGG ATT GCG ACA CTT AGT GGG) and NFATP2/3 (GGC GCA GAA AAG GTA AAA TAT TTA CTA TCT) binding sites within the IL-1RA promoter. PBMC RNA was isolated with an RNeasy kit (Qiagen, Germany) and messenger RNA was transcribed into cDNA with Reactin Ready First Strand kit (Biomol) and analysed for IL-1RA and GAPDH transcripts by PCR with a HotStart \"sweet\" PCR mastermix (Biomol) (95°C, 30 sec, 55°C, 30 sec, 72°C, 30 sec, 25 cycles for IL-1RA and 94°C for 30 sec, 50°C for 30 sec and 72°C for 45 sec, 25 cycles for GAPDH) following 18 h incubation with GP. IL-1RA protein was measured in the supernatant by ELISA after 24 h incubation with GP."}

test3

{"project":"test3","denotations":[{"id":"T20260","span":{"begin":1131,"end":1137},"obj":"Protein"},{"id":"T20259","span":{"begin":887,"end":898},"obj":"Transcription"},{"id":"T20258","span":{"begin":881,"end":886},"obj":"Protein"},{"id":"T20257","span":{"begin":870,"end":876},"obj":"Protein"},{"id":"T20256","span":{"begin":688,"end":694},"obj":"Protein"},{"id":"T20255","span":{"begin":619,"end":620},"obj":"Protein"},{"id":"T20254","span":{"begin":612,"end":618},"obj":"Protein"},{"id":"T20253","span":{"begin":559,"end":565},"obj":"Protein"},{"id":"T20252","span":{"begin":69,"end":75},"obj":"Protein"},{"id":"T20251","span":{"begin":29,"end":36},"obj":"Binding"},{"id":"T20250","span":{"begin":3,"end":10},"obj":"Negative_regulation"},{"id":"T20249","span":{"begin":1131,"end":1137},"obj":"Protein"},{"id":"T20248","span":{"begin":881,"end":886},"obj":"Protein"},{"id":"T20247","span":{"begin":870,"end":876},"obj":"Protein"},{"id":"T20246","span":{"begin":688,"end":694},"obj":"Protein"},{"id":"T20245","span":{"begin":619,"end":620},"obj":"Protein"},{"id":"T20244","span":{"begin":612,"end":618},"obj":"Protein"},{"id":"T20243","span":{"begin":559,"end":565},"obj":"Protein"},{"id":"T20242","span":{"begin":69,"end":75},"obj":"Protein"}],"relations":[{"id":"R13883","pred":"themeOf","subj":"T20251","obj":"T20250"},{"id":"R13884","pred":"themeOf","subj":"T20257","obj":"T20259"},{"id":"R13885","pred":"themeOf","subj":"T20258","obj":"T20259"}],"text":"To inhibit translocation and binding of transcription factors to the IL-1RA promoter, PBMC were incubated for 1 h with each of the following substances (concentrations were based upon 50% inhibition): 50 μg/ml of CAPE (caffeic-3,4-dihydroxycinnamic-acid-phenyl-ester), (Biomol, Hamburg, Germany); 400 ng/ml cyclosporin A (Calbiochem-Merck Biosciences, Bad Soden, Germany); 10 μg/ml cycloheximide (Santa Cruz Biotechnology) prior to incubation with GP. Band shifts were determined after 1 h incubation with GP as described before, including mutated oligos for NFIL-6 (TTA CAA CAG TGG ATT GCG ACA CTT AGT GGG) and NFATP2/3 (GGC GCA GAA AAG GTA AAA TAT TTA CTA TCT) binding sites within the IL-1RA promoter. PBMC RNA was isolated with an RNeasy kit (Qiagen, Germany) and messenger RNA was transcribed into cDNA with Reactin Ready First Strand kit (Biomol) and analysed for IL-1RA and GAPDH transcripts by PCR with a HotStart \"sweet\" PCR mastermix (Biomol) (95°C, 30 sec, 55°C, 30 sec, 72°C, 30 sec, 25 cycles for IL-1RA and 94°C for 30 sec, 50°C for 30 sec and 72°C for 45 sec, 25 cycles for GAPDH) following 18 h incubation with GP. IL-1RA protein was measured in the supernatant by ELISA after 24 h incubation with GP."}

PMC:1472690 / 38801-40018 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:1472690 / 38801-40018 JSON TXT