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PMC:1472690 / 33390-34131 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T17769 727-739 NN denotes BioWhittaker
T17768 726-727 -LRB- denotes (
T17767 714-725 NN denotes L-glutamine
T17766 711-713 NN denotes mM
T17765 707-710 CD denotes 200
T17764 705-706 NN denotes %
T17763 704-705 CD denotes 1
T17762 700-703 CC denotes and
T17761 698-699 -COMMA- denotes ,
T17760 697-698 -RRB- denotes )
T17759 692-697 NN denotes μg/ml
T17758 686-691 CD denotes 10000
T17757 685-686 -LRB- denotes (
T17756 672-684 NN denotes streptomycin
T17755 670-671 NN denotes %
T17754 669-670 CD denotes 1
T17753 667-668 -COMMA- denotes ,
T17752 666-667 -RRB- denotes )
T17751 662-666 NN denotes U/ml
T17750 656-661 CD denotes 10000
T17749 655-656 -LRB- denotes (
T17748 644-654 NN denotes penicillin
T17747 642-643 NN denotes %
T17746 641-642 CD denotes 1
T17745 639-640 -COMMA- denotes ,
T17744 638-639 -RRB- denotes )
T17743 631-638 NNP denotes Germany
T17742 629-630 -COMMA- denotes ,
T17741 620-629 NNP denotes Karlsruhe
T17740 618-619 -COMMA- denotes ,
T17739 608-618 NNP denotes Invitrogen
T17738 606-607 -COMMA- denotes ,
T17737 599-606 NN denotes low-LPS
T17736 597-598 -COMMA- denotes ,
T17735 594-597 NN denotes FCS
T17734 593-594 -LRB- denotes (
T17733 587-592 NN denotes serum
T17732 582-586 NN denotes calf
T17731 576-581 JJ denotes fetal
T17730 559-575 JJ denotes heat-inactivated
T17729 557-558 NN denotes %
T17728 555-557 CD denotes 10
T17727 544-554 VB denotes containing
T17726 542-543 -COMMA- denotes ,
T17725 541-542 -RRB- denotes )
T17724 533-541 JJ denotes LPS-free
T17723 531-532 -COMMA- denotes ,
T17722 524-531 NNP denotes Germany
T17721 522-523 -COMMA- denotes ,
T17720 512-522 NNP denotes Heidelberg
T17719 510-511 -COMMA- denotes ,
T17718 498-510 NNP denotes BioWhittaker
T17717 497-498 -LRB- denotes (
T17716 490-496 NN denotes medium
T17715 485-489 CD denotes 1640
T17714 480-484 NN denotes RPMI
T17713 477-479 IN denotes in
T17712 475-476 -RRB- denotes )
T17711 466-475 NN denotes cytometry
T17710 461-465 NN denotes flow
T17709 457-460 IN denotes for
T17708 456-457 -LRB- denotes (
T17707 447-455 NN denotes cells/ml
T17706 443-446 CD denotes 106
T17705 441-442 CC denotes ×
T17704 439-440 CD denotes 1
T17703 436-438 CC denotes or
T17702 434-435 -RRB- denotes )
T17701 428-434 NN denotes shifts
T17700 424-427 NN denotes gel
T17699 420-423 IN denotes for
T17698 419-420 -LRB- denotes (
T17697 412-418 NN denotes 106/ml
T17696 410-411 CC denotes ×
T17695 408-409 CD denotes 5
T17694 405-407 CC denotes or
T17693 403-404 -RRB- denotes )
T17692 391-403 NN denotes measurements
T17691 385-390 NN denotes ELISA
T17690 381-384 IN denotes for
T17689 380-381 -LRB- denotes (
T17688 371-379 NN denotes cells/ml
T17687 367-370 CD denotes 106
T17686 365-366 -COLON- denotes ×
T17685 363-364 CD denotes 3
T17684 360-362 IN denotes of
T17683 346-359 NN denotes concentration
T17682 340-345 JJ denotes final
T17681 338-339 DT denotes a
T17680 335-337 IN denotes at
T17679 326-334 VB denotes cultured
T17678 321-325 VB denotes were
T17677 316-320 NN denotes PBMC
T17676 314-315 -COMMA- denotes ,
T17675 305-314 NN denotes isolation
T17674 295-304 VB denotes Following
T17673 292-293 -RRB- denotes ]
T17672 287-292 CD denotes 54,55
T17671 286-287 -LRB- denotes [
T17670 277-285 NN denotes gradient
T17669 275-276 -RRB- denotes )
T17668 268-275 NNP denotes Germany
T17667 266-267 -COMMA- denotes ,
T17666 260-266 NNP denotes Berlin
T17665 258-259 -COMMA- denotes ,
T17664 250-258 NNP denotes Biochrom
T17663 249-250 -LRB- denotes (
T17662 234-248 JJ denotes Ficoll-Hypaque
T17661 232-233 DT denotes a
T17660 227-231 IN denotes over
T17659 217-226 VB denotes separated
T17658 212-216 VB denotes were
T17657 206-211 NN denotes coats
T17656 200-205 NN denotes buffy
T17655 196-199 DT denotes The
T17654 187-195 NNP denotes Luebeck.
T17653 184-186 IN denotes of
T17652 173-183 NNP denotes University
T17651 169-172 DT denotes the
T17650 166-168 IN denotes of
T17649 156-165 NN denotes committee
T17648 149-155 NN denotes ethics
T17647 145-148 DT denotes the
T17646 142-144 IN denotes of
T17645 133-141 NN denotes approval
T17644 129-132 DT denotes the
T17643 124-128 IN denotes with
T17642 120-123 CC denotes and
T17641 108-119 NN denotes declaration
T17640 99-107 NNP denotes Helsinki
T17639 95-98 DT denotes the
T17638 90-94 IN denotes with
T17637 79-89 NN denotes compliance
T17636 76-78 IN denotes in
T17635 69-75 NN denotes donors
T17634 63-68 NN denotes blood
T17633 55-62 JJ denotes healthy
T17632 50-54 IN denotes from
T17631 41-49 VB denotes obtained
T17630 36-40 VB denotes were
T17629 30-35 NN denotes coats
T17628 24-29 JJ denotes Buffy
T17627 19-23 NN denotes PBMC
T17626 13-18 JJ denotes human
T17625 10-12 IN denotes of
T17624 0-9 NN denotes Isolation
T17770 739-740 -RRB- denotes )
R11913 T17624 T17625 arg1Of Isolation,of
R11914 T17627 T17625 arg2Of PBMC,of
R11915 T17627 T17626 arg1Of PBMC,human
R11916 T17629 T17628 arg1Of coats,Buffy
R11917 T17629 T17630 arg1Of coats,were
R11918 T17629 T17631 arg2Of coats,obtained
R11919 T17631 T17630 arg2Of obtained,were
R11920 T17631 T17632 arg1Of obtained,from
R11921 T17631 T17643 arg1Of obtained,with
R11922 T17632 T17642 arg1Of from,and
R11923 T17635 T17632 arg2Of donors,from
R11924 T17635 T17633 arg1Of donors,healthy
R11925 T17635 T17634 arg1Of donors,blood
R11926 T17635 T17636 arg1Of donors,in
R11927 T17637 T17636 arg2Of compliance,in
R11928 T17637 T17638 arg1Of compliance,with
R11929 T17641 T17638 arg2Of declaration,with
R11930 T17641 T17639 arg1Of declaration,the
R11931 T17641 T17640 arg1Of declaration,Helsinki
R11932 T17643 T17642 arg2Of with,and
R11933 T17645 T17643 arg2Of approval,with
R11934 T17645 T17644 arg1Of approval,the
R11935 T17645 T17646 arg1Of approval,of
R11936 T17649 T17646 arg2Of committee,of
R11937 T17649 T17647 arg1Of committee,the
R11938 T17649 T17648 arg1Of committee,ethics
R11939 T17649 T17650 arg1Of committee,of
R11940 T17649 T17658 modOf committee,were
R11941 T17652 T17650 arg2Of University,of
R11942 T17652 T17651 arg1Of University,the
R11943 T17652 T17653 arg1Of University,of
R11944 T17654 T17653 arg2Of Luebeck.,of
R11945 T17657 T17655 arg1Of coats,The
R11946 T17657 T17656 arg1Of coats,buffy
R11947 T17657 T17658 arg1Of coats,were
R11948 T17657 T17659 arg2Of coats,separated
R11949 T17659 T17658 arg2Of separated,were
R11950 T17659 T17660 arg1Of separated,over
R11951 T17659 T17671 arg1Of separated,[
R11952 T17662 T17663 arg1Of Ficoll-Hypaque,(
R11953 T17664 T17665 arg1Of Biochrom,","
R11954 T17665 T17663 arg2Of ",",(
R11955 T17665 T17667 arg1Of ",",","
R11956 T17666 T17665 arg2Of Berlin,","
R11957 T17668 T17667 arg2Of Germany,","
R11958 T17669 T17663 arg3Of ),(
R11959 T17670 T17660 arg2Of gradient,over
R11960 T17670 T17661 arg1Of gradient,a
R11961 T17670 T17662 arg1Of gradient,Ficoll-Hypaque
R11962 T17672 T17671 arg2Of "54,55",[
R11963 T17673 T17671 arg3Of ],[
R11964 T17675 T17674 arg2Of isolation,Following
R11965 T17677 T17678 arg1Of PBMC,were
R11966 T17677 T17679 arg2Of PBMC,cultured
R11967 T17679 T17674 arg1Of cultured,Following
R11968 T17679 T17676 arg1Of cultured,","
R11969 T17679 T17678 arg2Of cultured,were
R11970 T17679 T17680 arg1Of cultured,at
R11971 T17683 T17680 arg2Of concentration,at
R11972 T17683 T17681 arg1Of concentration,a
R11973 T17683 T17682 arg1Of concentration,final
R11974 T17683 T17684 arg1Of concentration,of
R11975 T17685 T17684 arg2Of 3,of
R11976 T17688 T17687 arg1Of cells/ml,106
R11977 T17688 T17689 arg1Of cells/ml,(
R11978 T17688 T17694 arg1Of cells/ml,or
R11979 T17690 T17689 arg2Of for,(
R11980 T17692 T17690 arg2Of measurements,for
R11981 T17692 T17691 arg1Of measurements,ELISA
R11982 T17693 T17689 arg3Of ),(
R11983 T17694 T17703 arg1Of or,or
R11984 T17695 T17696 arg1Of 5,×
R11985 T17696 T17694 arg2Of ×,or
R11986 T17696 T17698 arg1Of ×,(
R11987 T17697 T17696 arg2Of 106/ml,×
R11988 T17699 T17698 arg2Of for,(
R11989 T17701 T17699 arg2Of shifts,for
R11990 T17701 T17700 arg1Of shifts,gel
R11991 T17702 T17698 arg3Of ),(
R11992 T17703 T17680 arg3Of or,at
R11993 T17703 T17686 arg1Of or,×
R11994 T17704 T17705 arg1Of 1,×
R11995 T17706 T17705 arg2Of 106,×
R11996 T17707 T17704 arg1Of cells/ml,1
R11997 T17707 T17706 arg1Of cells/ml,106
R11998 T17707 T17708 arg1Of cells/ml,(
R11999 T17707 T17713 arg1Of cells/ml,in
R12000 T17707 T17726 arg1Of cells/ml,","
R12001 T17707 T17727 arg1Of cells/ml,containing
R12002 T17707 T17734 arg1Of cells/ml,(
R12003 T17707 T17745 arg1Of cells/ml,","
R12004 T17709 T17708 arg2Of for,(
R12005 T17711 T17709 arg2Of cytometry,for
R12006 T17711 T17710 arg1Of cytometry,flow
R12007 T17712 T17708 arg3Of ),(
R12008 T17716 T17713 arg2Of medium,in
R12009 T17716 T17714 arg1Of medium,RPMI
R12010 T17716 T17715 arg1Of medium,1640
R12011 T17716 T17717 arg1Of medium,(
R12012 T17718 T17719 arg1Of BioWhittaker,","
R12013 T17719 T17721 arg1Of ",",","
R12014 T17720 T17719 arg2Of Heidelberg,","
R12015 T17721 T17717 arg2Of ",",(
R12016 T17721 T17723 arg1Of ",",","
R12017 T17721 T17724 arg1Of ",",LPS-free
R12018 T17722 T17721 arg2Of Germany,","
R12019 T17725 T17717 arg3Of ),(
R12020 T17728 T17729 arg1Of 10,%
R12021 T17733 T17727 arg2Of serum,containing
R12022 T17733 T17728 arg1Of serum,10
R12023 T17733 T17730 arg1Of serum,heat-inactivated
R12024 T17733 T17731 arg1Of serum,fetal
R12025 T17733 T17732 arg1Of serum,calf
R12026 T17735 T17736 arg1Of FCS,","
R12027 T17735 T17738 arg1Of FCS,","
R12028 T17735 T17740 arg1Of FCS,","
R12029 T17737 T17736 arg2Of low-LPS,","
R12030 T17739 T17738 arg2Of Invitrogen,","
R12031 T17740 T17734 arg2Of ",",(
R12032 T17741 T17740 arg2Of Karlsruhe,","
R12033 T17741 T17742 arg1Of Karlsruhe,","
R12034 T17743 T17742 arg2Of Germany,","
R12035 T17744 T17734 arg3Of ),(
R12036 T17745 T17753 arg1Of ",",","
R12037 T17746 T17747 arg1Of 1,%
R12038 T17748 T17745 arg2Of penicillin,","
R12039 T17748 T17746 arg1Of penicillin,1
R12040 T17748 T17749 arg1Of penicillin,(
R12041 T17751 T17749 arg2Of U/ml,(
R12042 T17751 T17750 arg1Of U/ml,10000
R12043 T17752 T17749 arg3Of ),(
R12044 T17753 T17762 arg1Of ",",and
R12045 T17754 T17755 arg1Of 1,%
R12046 T17756 T17753 arg2Of streptomycin,","
R12047 T17756 T17754 arg1Of streptomycin,1
R12048 T17756 T17757 arg1Of streptomycin,(
R12049 T17759 T17757 arg2Of μg/ml,(
R12050 T17759 T17758 arg1Of μg/ml,10000
R12051 T17760 T17757 arg3Of ),(
R12052 T17762 T17703 arg2Of and,or
R12053 T17762 T17761 arg1Of and,","
R12054 T17765 T17766 arg1Of 200,mM
R12055 T17767 T17762 arg2Of L-glutamine,and
R12056 T17767 T17763 arg1Of L-glutamine,1
R12057 T17767 T17764 arg1Of L-glutamine,%
R12058 T17767 T17765 arg1Of L-glutamine,200
R12059 T17767 T17768 arg1Of L-glutamine,(
R12060 T17769 T17768 arg2Of BioWhittaker,(
R12061 T17770 T17768 arg3Of ),(

UBERON-AE

Id Subject Object Predicate Lexical cue
T17617 587-592 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T17616 63-68 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood

GO-CC

Id Subject Object Predicate Lexical cue
T17623 447-452 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17622 371-376 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T17620 295-741 Sentence denotes Following isolation, PBMC were cultured at a final concentration of 3 × 106 cells/ml (for ELISA measurements) or 5 × 106/ml (for gel shifts) or 1 × 106 cells/ml (for flow cytometry) in RPMI 1640 medium (BioWhittaker, Heidelberg, Germany, LPS-free), containing 10% heat-inactivated fetal calf serum (FCS, low-LPS, Invitrogen, Karlsruhe, Germany), 1% penicillin (10000 U/ml), 1% streptomycin (10000 μg/ml), and 1% 200 mM L-glutamine (BioWhittaker).
T17619 24-294 Sentence denotes Buffy coats were obtained from healthy blood donors in compliance with the Helsinki declaration and with the approval of the ethics committee of the University of Luebeck. The buffy coats were separated over a Ficoll-Hypaque (Biochrom, Berlin, Germany) gradient [54,55].
T17618 0-23 Sentence denotes Isolation of human PBMC
T231 0-23 Sentence denotes Isolation of human PBMC
T232 24-195 Sentence denotes Buffy coats were obtained from healthy blood donors in compliance with the Helsinki declaration and with the approval of the ethics committee of the University of Luebeck.
T233 196-294 Sentence denotes The buffy coats were separated over a Ficoll-Hypaque (Biochrom, Berlin, Germany) gradient [54,55].
T234 295-741 Sentence denotes Following isolation, PBMC were cultured at a final concentration of 3 × 106 cells/ml (for ELISA measurements) or 5 × 106/ml (for gel shifts) or 1 × 106 cells/ml (for flow cytometry) in RPMI 1640 medium (BioWhittaker, Heidelberg, Germany, LPS-free), containing 10% heat-inactivated fetal calf serum (FCS, low-LPS, Invitrogen, Karlsruhe, Germany), 1% penicillin (10000 U/ml), 1% streptomycin (10000 μg/ml), and 1% 200 mM L-glutamine (BioWhittaker).

ICD10

Id Subject Object Predicate Lexical cue
T17621 63-75 http://purl.bioontology.org/ontology/ICD10/Z52.0 denotes blood donors

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T17930 641-654 Entity denotes 1% penicillin
T17929 704-740 Entity denotes 1% 200 mM L-glutamine (BioWhittaker)
T17928 10-23 Entity denotes of human PBMC
T17927 316-320 Entity denotes PBMC
T17926 424-427 Entity denotes gel
T17925 669-699 Entity denotes 1% streptomycin (10000 μg/ml),
T17924 599-606 Entity denotes low-LPS
T17923 533-541 Entity denotes LPS-free

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T17922 740-741 . denotes .
T17921 739-740 -RRB- denotes )
T17920 727-739 NNP denotes BioWhittaker
T17919 726-727 -LRB- denotes (
T17918 714-725 NNP denotes L-glutamine
T17917 711-713 NNP denotes mM
T17916 707-710 CD denotes 200
T17915 705-706 NN denotes %
T17914 704-705 CD denotes 1
T17913 700-703 CC denotes and
T17912 698-699 , denotes ,
T17911 697-698 -RRB- denotes )
T17910 695-697 NN denotes ml
T17909 694-695 NN denotes /
T17908 692-694 NN denotes μg
T17907 686-691 CD denotes 10000
T17906 685-686 -LRB- denotes (
T17905 672-684 NN denotes streptomycin
T17904 670-671 NN denotes %
T17903 669-670 CD denotes 1
T17902 667-668 , denotes ,
T17901 666-667 -RRB- denotes )
T17900 662-666 NNP denotes U/ml
T17899 656-661 CD denotes 10000
T17898 655-656 -LRB- denotes (
T17897 644-654 NN denotes penicillin
T17896 642-643 NN denotes %
T17895 641-642 CD denotes 1
T17894 639-640 , denotes ,
T17893 638-639 -RRB- denotes )
T17892 631-638 NNP denotes Germany
T17891 629-630 , denotes ,
T17890 620-629 NNP denotes Karlsruhe
T17889 618-619 , denotes ,
T17888 608-618 NNP denotes Invitrogen
T17887 606-607 , denotes ,
T17886 599-606 NNP denotes low-LPS
T17885 597-598 , denotes ,
T17884 594-597 NNP denotes FCS
T17883 593-594 -LRB- denotes (
T17882 587-592 NN denotes serum
T17881 582-586 NN denotes calf
T17880 576-581 JJ denotes fetal
T17879 559-575 JJ denotes heat-inactivated
T17878 557-558 NN denotes %
T17877 555-557 CD denotes 10
T17876 544-554 VBG denotes containing
T17875 542-543 , denotes ,
T17874 541-542 -RRB- denotes )
T17873 533-541 JJ denotes LPS-free
T17872 531-532 , denotes ,
T17871 524-531 NNP denotes Germany
T17870 522-523 , denotes ,
T17869 512-522 NNP denotes Heidelberg
T17868 510-511 , denotes ,
T17867 498-510 NNP denotes BioWhittaker
T17866 497-498 -LRB- denotes (
T17865 490-496 NN denotes medium
T17864 485-489 CD denotes 1640
T17863 480-484 NNP denotes RPMI
T17862 477-479 IN denotes in
T17861 475-476 -RRB- denotes )
T17860 466-475 NN denotes cytometry
T17859 461-465 NN denotes flow
T17858 457-460 IN denotes for
T17857 456-457 -LRB- denotes (
T17856 447-455 NN denotes cells/ml
T17855 443-446 CD denotes 106
T17854 441-442 CD denotes ×
T17853 439-440 CD denotes 1
T17852 436-438 CC denotes or
T17851 434-435 -RRB- denotes )
T17850 428-434 NNS denotes shifts
T17849 424-427 NN denotes gel
T17848 420-423 IN denotes for
T17847 419-420 -LRB- denotes (
T17846 412-418 CD denotes 106/ml
T17845 410-411 NN denotes ×
T17844 408-409 CD denotes 5
T17843 405-407 CC denotes or
T17842 403-404 -RRB- denotes )
T17841 391-403 NNS denotes measurements
T17840 385-390 NNP denotes ELISA
T17839 381-384 IN denotes for
T17838 380-381 -LRB- denotes (
T17837 371-379 NN denotes cells/ml
T17836 367-370 CD denotes 106
T17835 365-366 CD denotes ×
T17834 363-364 CD denotes 3
T17833 360-362 IN denotes of
T17832 346-359 NN denotes concentration
T17831 340-345 JJ denotes final
T17830 338-339 DT denotes a
T17829 335-337 IN denotes at
T17828 326-334 VBN denotes cultured
T17827 321-325 VBD denotes were
T17826 316-320 NNP denotes PBMC
T17825 314-315 , denotes ,
T17824 305-314 NN denotes isolation
T17823 295-304 VBG denotes Following
T17822 293-294 . denotes .
T17821 292-293 NNP denotes ]
T17820 287-292 CD denotes 54,55
T17819 286-287 NNP denotes [
T17818 277-285 NN denotes gradient
T17817 275-276 -RRB- denotes )
T17816 268-275 NNP denotes Germany
T17815 266-267 , denotes ,
T17814 260-266 NNP denotes Berlin
T17813 258-259 , denotes ,
T17812 250-258 NNP denotes Biochrom
T17811 249-250 -LRB- denotes (
T17810 234-248 NNP denotes Ficoll-Hypaque
T17809 232-233 DT denotes a
T17808 227-231 IN denotes over
T17807 217-226 VBN denotes separated
T17806 212-216 VBD denotes were
T17805 206-211 NNS denotes coats
T17804 200-205 JJ denotes buffy
T17803 196-199 DT denotes The
T17802 194-195 . denotes .
T17801 187-194 NNP denotes Luebeck
T17800 184-186 IN denotes of
T17799 173-183 NNP denotes University
T17798 169-172 DT denotes the
T17797 166-168 IN denotes of
T17796 156-165 NN denotes committee
T17795 149-155 NNS denotes ethics
T17794 145-148 DT denotes the
T17793 142-144 IN denotes of
T17792 133-141 NN denotes approval
T17791 129-132 DT denotes the
T17790 124-128 IN denotes with
T17789 120-123 CC denotes and
T17788 108-119 NN denotes declaration
T17787 99-107 NNP denotes Helsinki
T17786 95-98 DT denotes the
T17785 90-94 IN denotes with
T17784 79-89 NN denotes compliance
T17783 76-78 IN denotes in
T17782 69-75 NNS denotes donors
T17781 63-68 NN denotes blood
T17780 55-62 JJ denotes healthy
T17779 50-54 IN denotes from
T17778 41-49 VBN denotes obtained
T17777 36-40 VBD denotes were
T17776 30-35 NNS denotes coats
T17775 24-29 NNP denotes Buffy
T17774 19-23 NNP denotes PBMC
T17773 13-18 JJ denotes human
T17772 10-12 IN denotes of
T17771 0-9 NN denotes Isolation
R12086 T17795 T17796 compound ethics,committee
R12087 T17796 T17793 pobj committee,of
R12088 T17797 T17796 prep of,committee
R12089 T17798 T17799 det the,University
R12090 T17799 T17797 pobj University,of
R12091 T17800 T17799 prep of,University
R12092 T17801 T17800 pobj Luebeck,of
R12093 T17802 T17778 punct .,obtained
R12094 T17803 T17805 det The,coats
R12095 T17804 T17805 amod buffy,coats
R12096 T17805 T17807 nsubjpass coats,separated
R12097 T17806 T17807 auxpass were,separated
R12098 T17807 T17807 ROOT separated,separated
R12099 T17808 T17807 prep over,separated
R12100 T17809 T17810 det a,Ficoll-Hypaque
R12101 T17810 T17808 pobj Ficoll-Hypaque,over
R12102 T17811 T17818 punct (,gradient
R12103 T17812 T17818 nmod Biochrom,gradient
R12104 T17813 T17812 punct ",",Biochrom
R12105 T17814 T17812 conj Berlin,Biochrom
R12106 T17815 T17814 punct ",",Berlin
R12107 T17816 T17814 conj Germany,Berlin
R12108 T17817 T17818 punct ),gradient
R12145 T17854 T17856 nummod ×,cells/ml
R12146 T17855 T17856 nummod 106,cells/ml
R12147 T17856 T17845 conj cells/ml,×
R12148 T17857 T17858 punct (,for
R12149 T17858 T17856 prep for,cells/ml
R12150 T17859 T17860 compound flow,cytometry
R12151 T17860 T17858 pobj cytometry,for
R12182 T17891 T17890 punct ",",Karlsruhe
R12183 T17892 T17890 conj Germany,Karlsruhe
R12184 T17893 T17890 punct ),Karlsruhe
R12185 T17894 T17888 punct ",",Invitrogen
R12186 T17895 T17896 nummod 1,%
R12187 T17896 T17897 compound %,penicillin
R12188 T17897 T17888 appos penicillin,Invitrogen
R12189 T17898 T17900 punct (,U/ml
R12190 T17899 T17900 nummod 10000,U/ml
R12191 T17900 T17897 appos U/ml,penicillin
R12192 T17901 T17900 punct ),U/ml
R12193 T17902 T17897 punct ",",penicillin
R12194 T17903 T17904 nummod 1,%
R12195 T17904 T17905 compound %,streptomycin
R12196 T17905 T17882 appos streptomycin,serum
R12197 T17906 T17905 punct (,streptomycin
R12198 T17907 T17910 nummod 10000,ml
R12199 T17908 T17910 compound μg,ml
R12200 T17909 T17910 compound /,ml
R12201 T17910 T17905 appos ml,streptomycin
R12202 T17911 T17905 punct ),streptomycin
R12203 T17912 T17905 punct ",",streptomycin
R12204 T17913 T17882 cc and,serum
R12205 T17914 T17915 nummod 1,%
R12206 T17915 T17876 npadvmod %,containing
R12207 T17916 T17918 nummod 200,L-glutamine
R12208 T17917 T17918 compound mM,L-glutamine
R12209 T17918 T17918 ROOT L-glutamine,L-glutamine
R12210 T17919 T17920 punct (,BioWhittaker
R12211 T17920 T17918 appos BioWhittaker,L-glutamine
R12212 T17921 T17918 punct ),L-glutamine
R12213 T17922 T17828 punct .,cultured
R12062 T17771 T17778 nsubjpass Isolation,obtained
R12063 T17772 T17771 prep of,Isolation
R12064 T17773 T17776 amod human,coats
R12065 T17774 T17775 compound PBMC,Buffy
R12066 T17775 T17776 compound Buffy,coats
R12067 T17776 T17772 pobj coats,of
R12068 T17777 T17778 auxpass were,obtained
R12069 T17778 T17778 ROOT obtained,obtained
R12070 T17779 T17778 prep from,obtained
R12071 T17780 T17782 amod healthy,donors
R12072 T17781 T17782 compound blood,donors
R12073 T17782 T17779 pobj donors,from
R12074 T17783 T17778 prep in,obtained
R12075 T17784 T17783 pobj compliance,in
R12076 T17785 T17784 prep with,compliance
R12077 T17786 T17788 det the,declaration
R12078 T17787 T17788 compound Helsinki,declaration
R12079 T17788 T17785 pobj declaration,with
R12080 T17789 T17785 cc and,with
R12081 T17790 T17785 conj with,with
R12082 T17791 T17792 det the,approval
R12083 T17792 T17790 pobj approval,with
R12084 T17793 T17792 prep of,approval
R12085 T17794 T17796 det the,committee
R12109 T17818 T17821 compound gradient,]
R12110 T17819 T17821 compound [,]
R12111 T17820 T17821 compound "54,55",]
R12112 T17821 T17807 npadvmod ],separated
R12113 T17822 T17807 punct .,separated
R12114 T17823 T17828 prep Following,cultured
R12115 T17824 T17823 pobj isolation,Following
R12116 T17825 T17828 punct ",",cultured
R12117 T17826 T17828 nsubjpass PBMC,cultured
R12118 T17827 T17828 auxpass were,cultured
R12119 T17828 T17828 ROOT cultured,cultured
R12120 T17829 T17828 prep at,cultured
R12121 T17830 T17832 det a,concentration
R12122 T17831 T17832 amod final,concentration
R12123 T17832 T17829 pobj concentration,at
R12124 T17833 T17832 prep of,concentration
R12125 T17834 T17837 nummod 3,cells/ml
R12126 T17835 T17837 nummod ×,cells/ml
R12127 T17836 T17837 nummod 106,cells/ml
R12128 T17837 T17833 pobj cells/ml,of
R12129 T17838 T17837 punct (,cells/ml
R12130 T17839 T17837 prep for,cells/ml
R12131 T17840 T17841 compound ELISA,measurements
R12132 T17841 T17839 pobj measurements,for
R12133 T17842 T17837 punct ),cells/ml
R12134 T17843 T17832 cc or,concentration
R12135 T17844 T17845 nummod 5,×
R12136 T17845 T17876 nmod ×,containing
R12137 T17846 T17845 nummod 106/ml,×
R12138 T17847 T17848 punct (,for
R12139 T17848 T17845 prep for,×
R12140 T17849 T17850 compound gel,shifts
R12141 T17850 T17848 pobj shifts,for
R12142 T17851 T17848 punct ),for
R12143 T17852 T17845 cc or,×
R12144 T17853 T17856 nummod 1,cells/ml
R12152 T17861 T17858 punct ),for
R12153 T17862 T17856 prep in,cells/ml
R12154 T17863 T17865 nmod RPMI,medium
R12155 T17864 T17865 nummod 1640,medium
R12156 T17865 T17862 pobj medium,in
R12157 T17866 T17876 punct (,containing
R12158 T17867 T17876 dep BioWhittaker,containing
R12159 T17868 T17867 punct ",",BioWhittaker
R12160 T17869 T17867 conj Heidelberg,BioWhittaker
R12161 T17870 T17869 punct ",",Heidelberg
R12162 T17871 T17867 npadvmod Germany,BioWhittaker
R12163 T17872 T17867 punct ",",BioWhittaker
R12164 T17873 T17867 appos LPS-free,BioWhittaker
R12165 T17874 T17873 punct ),LPS-free
R12166 T17875 T17876 punct ",",containing
R12167 T17876 T17828 advcl containing,cultured
R12168 T17877 T17878 nummod 10,%
R12169 T17878 T17882 nmod %,serum
R12170 T17879 T17882 amod heat-inactivated,serum
R12171 T17880 T17881 amod fetal,calf
R12172 T17881 T17882 compound calf,serum
R12173 T17882 T17876 dobj serum,containing
R12174 T17883 T17905 punct (,streptomycin
R12175 T17884 T17905 nmod FCS,streptomycin
R12176 T17885 T17884 punct ",",FCS
R12177 T17886 T17884 conj low-LPS,FCS
R12178 T17887 T17886 punct ",",low-LPS
R12179 T17888 T17886 conj Invitrogen,low-LPS
R12180 T17889 T17888 punct ",",Invitrogen
R12181 T17890 T17888 conj Karlsruhe,Invitrogen