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PMC:1472690 / 32969-40917 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T21025 7122-7129 IN denotes whether
T21024 7120-7121 -COMMA- denotes ,
T21023 7112-7120 VB denotes evaluate
T21022 7109-7111 TO denotes to
T21021 7104-7108 VB denotes used
T21020 7100-7103 VB denotes was
T21019 7095-7099 NN denotes test
T21018 7076-7094 NNP denotes Kolmogorow-Smirnov
T21017 7072-7075 DT denotes The
T21016 7063-7071 NN denotes analysis
T21015 7051-7062 JJ denotes Statistical
T20575 7046-7048 NN denotes GP
T20574 7041-7045 IN denotes with
T20573 7030-7040 NN denotes incubation
T20572 7028-7029 NN denotes h
T20571 7025-7027 CD denotes 24
T20570 7019-7024 IN denotes after
T20569 7013-7018 NN denotes ELISA
T20568 7010-7012 IN denotes by
T20567 6998-7009 NN denotes supernatant
T20566 6994-6997 DT denotes the
T20565 6991-6993 IN denotes in
T20564 6982-6990 VB denotes measured
T20563 6978-6981 VB denotes was
T20562 6970-6977 NN denotes protein
T20561 6963-6969 NN denotes IL-1RA
T20560 6959-6961 NN denotes GP
T20559 6954-6958 IN denotes with
T20558 6943-6953 NN denotes incubation
T20557 6941-6942 NN denotes h
T20556 6938-6940 CD denotes 18
T20555 6928-6937 VB denotes following
T20554 6926-6927 -RRB- denotes )
T20553 6921-6926 NN denotes GAPDH
T20552 6917-6920 IN denotes for
T20551 6910-6916 NN denotes cycles
T20550 6907-6909 CD denotes 25
T20549 6905-6906 -COMMA- denotes ,
T20548 6902-6905 NN denotes sec
T20547 6899-6901 CD denotes 45
T20546 6895-6898 IN denotes for
T20545 6890-6894 NN denotes 72°C
T20544 6886-6889 CC denotes and
T20543 6882-6885 NN denotes sec
T20542 6879-6881 CD denotes 30
T20541 6875-6878 IN denotes for
T20540 6870-6874 NN denotes 50°C
T20539 6868-6869 -COMMA- denotes ,
T20538 6865-6868 NN denotes sec
T20537 6862-6864 CD denotes 30
T20536 6858-6861 IN denotes for
T20535 6853-6857 NN denotes 94°C
T20534 6849-6852 CC denotes and
T20533 6842-6848 NN denotes IL-1RA
T20532 6838-6841 IN denotes for
T20531 6831-6837 NN denotes cycles
T20530 6828-6830 CD denotes 25
T20529 6826-6827 -COMMA- denotes ,
T20528 6823-6826 NN denotes sec
T20527 6820-6822 CD denotes 30
T20526 6818-6819 -COMMA- denotes ,
T20525 6814-6818 NN denotes 72°C
T20524 6812-6813 -COMMA- denotes ,
T20523 6809-6812 NN denotes sec
T20522 6806-6808 CD denotes 30
T20521 6804-6805 -COMMA- denotes ,
T20520 6800-6804 NN denotes 55°C
T20519 6798-6799 -COMMA- denotes ,
T20518 6795-6798 NN denotes sec
T20517 6792-6794 CD denotes 30
T20516 6790-6791 -COMMA- denotes ,
T20515 6786-6790 NN denotes 95°C
T20514 6785-6786 -LRB- denotes (
T20513 6783-6784 -RRB- denotes )
T20512 6777-6783 NN denotes Biomol
T20511 6776-6777 -LRB- denotes (
T20510 6766-6775 NN denotes mastermix
T20509 6762-6765 NN denotes PCR
T20508 6760-6761 -DQE- denotes "
T20507 6755-6760 JJ denotes sweet
T20506 6754-6755 -DQS- denotes "
T20505 6745-6753 NNP denotes HotStart
T20504 6743-6744 DT denotes a
T20503 6738-6742 IN denotes with
T20502 6734-6737 NN denotes PCR
T20501 6731-6733 IN denotes by
T20500 6719-6730 NN denotes transcripts
T20499 6713-6718 NN denotes GAPDH
T20498 6709-6712 CC denotes and
T20497 6702-6708 NN denotes IL-1RA
T20496 6698-6701 IN denotes for
T20495 6689-6697 VB denotes analysed
T20494 6685-6688 CC denotes and
T20493 6683-6684 -RRB- denotes )
T20492 6677-6683 NN denotes Biomol
T20491 6676-6677 -LRB- denotes (
T20490 6672-6675 NN denotes kit
T20489 6665-6671 NNP denotes Strand
T20488 6659-6664 NNP denotes First
T20487 6653-6658 NNP denotes Ready
T20486 6645-6652 NN denotes Reactin
T20485 6640-6644 IN denotes with
T20484 6635-6639 NN denotes cDNA
T20483 6630-6634 IN denotes into
T20482 6618-6629 VB denotes transcribed
T20481 6614-6617 VB denotes was
T20480 6610-6613 NN denotes RNA
T20479 6600-6609 NN denotes messenger
T20478 6596-6599 CC denotes and
T20477 6594-6595 -RRB- denotes )
T20476 6587-6594 NNP denotes Germany
T20475 6585-6586 -COMMA- denotes ,
T20474 6579-6585 NN denotes Qiagen
T20473 6578-6579 -LRB- denotes (
T20472 6574-6577 NN denotes kit
T20471 6567-6573 NN denotes RNeasy
T20470 6564-6566 DT denotes an
T20469 6559-6563 IN denotes with
T20468 6550-6558 VB denotes isolated
T20467 6546-6549 VB denotes was
T20466 6542-6545 NN denotes RNA
T20465 6537-6541 NN denotes PBMC
T20464 6527-6535 NN denotes promoter
T20463 6520-6526 NN denotes IL-1RA
T20462 6516-6519 DT denotes the
T20461 6509-6515 IN denotes within
T20460 6503-6508 NN denotes sites
T20459 6495-6502 NN denotes binding
T20458 6493-6494 -RRB- denotes )
T20457 6490-6493 NN denotes TCT
T20456 6486-6489 NN denotes CTA
T20455 6482-6485 NN denotes TTA
T20454 6478-6481 NN denotes TAT
T20453 6474-6477 NN denotes AAA
T21167 7946-7947 -RRB- denotes )
T21166 7939-7946 NNP denotes Germany
T21165 7937-7938 -COMMA- denotes ,
T21164 7930-7937 NNP denotes Erkrath
T21163 7928-7929 -COMMA- denotes ,
T21162 7920-7928 NNP denotes Software
T21161 7912-7919 NNP denotes Science
T21160 7907-7911 NNP denotes SPSS
T21159 7905-7906 -COLON- denotes ;
T21158 7898-7905 NNP denotes Windows
T21157 7894-7897 IN denotes for
T21156 7889-7893 NN denotes SPSS
T21155 7888-7889 -LRB- denotes (
T21154 7886-7887 -COMMA- denotes ,
T21153 7885-7886 -RRB- denotes )
T21152 7873-7885 NN denotes distribution
T21151 7862-7872 JJ denotes non-normal
T21150 7861-7862 -LRB- denotes (
T21149 7856-7860 NN denotes test
T21148 7844-7855 NNP denotes Signed-Rank
T21147 7835-7843 NNP denotes Wilcoxon
T21146 7831-7834 DT denotes the
T21145 7828-7830 CC denotes or
T21144 7826-7827 -RRB- denotes )
T21143 7814-7826 NN denotes distribution
T21142 7807-7813 JJ denotes normal
T21141 7806-7807 -LRB- denotes (
T21140 7799-7805 NN denotes t-test
T21139 7796-7798 POS denotes 's
T21138 7789-7796 NNP denotes Student
T21137 7785-7788 DT denotes the
T21136 7779-7784 VB denotes using
T21135 7770-7778 VB denotes analysed
T21134 7765-7769 VB denotes were
T21133 7763-7764 -RRB- denotes )
T21132 7758-7763 NN denotes calc.
T21131 7748-7757 NN denotes GP/TSST-1
T21130 7745-7747 IN denotes vs
T21129 7738-7744 NN denotes TSST-1
T21128 7736-7737 SYM denotes +
T21127 7733-7735 NN denotes GP
T21126 7725-7732 NN denotes example
T21125 7721-7724 IN denotes for
T21124 7720-7721 -LRB- denotes (
T21123 7713-7719 NN denotes values
T21122 7701-7712 JJ denotes theoretical
T21121 7697-7700 CC denotes and
T21120 7692-7696 NN denotes data
T21119 7679-7691 JJ denotes experimental
T21118 7671-7678 IN denotes between
T21117 7668-7670 IN denotes as
T21116 7663-7667 RB denotes well
T21115 7660-7662 RB denotes as
T21114 7658-7659 -RRB- denotes )
T21113 7655-7658 NN denotes LPS
T21112 7652-7654 IN denotes vs
T21111 7649-7651 NN denotes GP
T21110 7641-7648 NN denotes example
T21109 7637-7640 IN denotes for
T21108 7636-7637 -LRB- denotes (
T21107 7631-7635 NN denotes data
T21106 7618-7630 JJ denotes experimental
T21105 7610-7617 IN denotes between
T21104 7598-7609 NN denotes differences
T21103 7595-7597 IN denotes of
T21102 7581-7594 NN denotes Significances
T21101 7578-7579 CD denotes 6
T21100 7574-7577 CC denotes and
T21099 7572-7573 -COMMA- denotes ,
T21098 7571-7572 CD denotes 5
T21097 7569-7570 -COMMA- denotes ,
T21096 7568-7569 CD denotes 4
T21095 7566-7567 -COMMA- denotes ,
T21094 7565-7566 CD denotes 3
T21093 7563-7564 -COMMA- denotes ,
T21092 7561-7563 NN denotes 1B
T21091 7556-7560 NNP denotes Fig.
T21090 7551-7555 IN denotes into
T21089 7544-7550 NN denotes values
T21088 7538-7543 DT denotes these
T21087 7532-7537 VB denotes added
T21086 7528-7531 CC denotes and
T21085 7520-7527 NN denotes effects
T21084 7509-7519 JJ denotes individual
T21083 7505-7508 DT denotes the
T21082 7502-7504 IN denotes of
T21081 7498-7501 NN denotes sum
T21080 7494-7497 DT denotes the
T21079 7483-7493 VB denotes calculated
T21078 7480-7482 PRP denotes we
T21077 7478-7479 -RRB- denotes )
T21076 7473-7478 NN denotes calc.
T21075 7463-7472 NN denotes GP/TSST-1
T21074 7460-7462 IN denotes vs
T21073 7453-7459 NN denotes TSST-1
T21072 7451-7452 SYM denotes +
T21071 7448-7450 NN denotes GP
T21070 7440-7447 NN denotes example
T21069 7436-7439 IN denotes for
T21068 7435-7436 -LRB- denotes (
T21067 7429-7434 NN denotes value
T21066 7417-7428 JJ denotes theoretical
T21065 7415-7416 DT denotes a
T21064 7412-7414 TO denotes to
T21063 7407-7411 NN denotes data
T21062 7394-7406 JJ denotes experimental
T21061 7386-7393 VB denotes compare
T21060 7383-7385 TO denotes To
T21059 7380-7381 -RRB- denotes )
T21058 7368-7380 NN denotes distribution
T21057 7357-7367 JJ denotes non-normal
T21056 7356-7357 -LRB- denotes (
T21055 7351-7355 NN denotes test
T21054 7342-7350 NNP denotes Spearman
T21053 7338-7341 DT denotes the
T21052 7335-7337 CC denotes or
T21051 7333-7334 -RRB- denotes )
T21050 7321-7333 NN denotes distribution
T21049 7314-7320 JJ denotes normal
T21048 7313-7314 -LRB- denotes (
T21047 7308-7312 NN denotes test
T21046 7300-7307 NNP denotes Pearson
T21045 7296-7299 DT denotes the
T21044 7293-7295 IN denotes by
T21043 7284-7292 VB denotes analyzed
T21042 7279-7283 VB denotes were
T21041 7265-7278 NN denotes significances
T21040 7251-7264 JJ denotes corresponding
T21039 7247-7250 CC denotes and
T21038 7234-7246 NN denotes coefficients
T21037 7222-7233 NN denotes Correlation
T21036 7209-7220 VB denotes distributed
T21035 7200-7208 RB denotes normally
T21034 7195-7199 VB denotes were
T21033 7187-7194 NN denotes factors
T21032 7173-7186 NN denotes transcription
T21031 7170-7172 TO denotes to
T21030 7159-7169 NN denotes activities
T21029 7151-7158 NN denotes binding
T21028 7147-7150 CC denotes and
T21027 7139-7146 NN denotes amounts
T21026 7130-7138 NN denotes cytokine
T20452 6470-6473 NN denotes GTA
T20451 6466-6469 NN denotes AAG
T20450 6462-6465 NN denotes GAA
T20449 6458-6461 NN denotes GCA
T20448 6454-6457 NN denotes GGC
T20447 6453-6454 -LRB- denotes (
T20446 6444-6452 NN denotes NFATP2/3
T20445 6440-6443 CC denotes and
T20444 6438-6439 -RRB- denotes )
T20443 6435-6438 NN denotes GGG
T20442 6431-6434 NNP denotes AGT
T20441 6427-6430 NNP denotes CTT
T20440 6423-6426 NNP denotes ACA
T20439 6419-6422 NNP denotes GCG
T20438 6415-6418 NNP denotes ATT
T20437 6411-6414 NNP denotes TGG
T20436 6407-6410 NNP denotes CAG
T20435 6403-6406 NN denotes CAA
T20434 6399-6402 NN denotes TTA
T20433 6398-6399 -LRB- denotes (
T20432 6391-6397 NN denotes NFIL-6
T20431 6387-6390 IN denotes for
T20430 6380-6386 NN denotes oligos
T20429 6372-6379 VB denotes mutated
T20428 6362-6371 VB denotes including
T20427 6360-6361 -COMMA- denotes ,
T20370 6031-6032 -COLON- denotes :
T20369 6030-6031 -RRB- denotes )
T20368 6020-6030 NN denotes inhibition
T20367 6018-6019 NN denotes %
T20366 6016-6018 CD denotes 50
T20365 6011-6015 IN denotes upon
T20364 6005-6010 VB denotes based
T20363 6000-6004 VB denotes were
T20362 5985-5999 NN denotes concentrations
T20361 5984-5985 -LRB- denotes (
T20360 5973-5983 NN denotes substances
T20359 5963-5972 VB denotes following
T20358 5959-5962 DT denotes the
T20357 5956-5958 IN denotes of
T20356 5951-5955 DT denotes each
T20355 5946-5950 IN denotes with
T20354 5944-5945 NN denotes h
T20353 5942-5943 CD denotes 1
T20352 5938-5941 IN denotes for
T20351 5928-5937 VB denotes incubated
T20350 5923-5927 VB denotes were
T20349 5918-5922 NN denotes PBMC
T20348 5916-5917 -COMMA- denotes ,
T20347 5908-5916 NN denotes promoter
T20346 5901-5907 NN denotes IL-1RA
T20345 5897-5900 DT denotes the
T20344 5894-5896 TO denotes to
T20343 5886-5893 NN denotes factors
T20342 5872-5885 NN denotes transcription
T20341 5869-5871 IN denotes of
T20340 5861-5868 NN denotes binding
T20339 5857-5860 CC denotes and
T20338 5843-5856 NN denotes translocation
T20337 5835-5842 VB denotes inhibit
T20336 5832-5834 TO denotes To
T20335 5824-5831 NN denotes factors
T20334 5810-5823 NN denotes transcription
T20333 5807-5809 IN denotes of
T20332 5796-5806 NN denotes Inhibition
T19971 5783-5793 NN denotes statistics
T19970 5779-5782 DT denotes the
T19969 5776-5778 IN denotes in
T19968 5767-5775 VB denotes included
T19967 5763-5766 VB denotes was
T19966 5755-5762 NN denotes release
T19965 5746-5754 NN denotes cytokine
T19964 5734-5745 JJ denotes spontaneous
T19963 5731-5733 DT denotes no
T19962 5723-5730 VB denotes showing
T19961 5716-5722 NN denotes donors
T19960 5713-5715 IN denotes in
T19959 5704-5712 VB denotes observed
T19958 5699-5703 NN denotes PBMC
T19957 5696-5698 IN denotes of
T19956 5684-5695 NN denotes stimulation
T19955 5679-5683 RB denotes Only
T19954 5672-5677 NN denotes curve
T19953 5663-5671 JJ denotes standard
T19952 5659-5662 DT denotes the
T19951 5656-5658 IN denotes of
T19950 5650-5655 NN denotes range
T19949 5646-5649 DT denotes the
T19948 5639-5645 IN denotes within
T19947 5635-5638 DT denotes all
T19946 5630-5634 VB denotes were
T19945 5622-5629 NN denotes amounts
T19944 5613-5621 NN denotes Cytokine
T19943 5610-5611 -RRB- denotes )
T19942 5604-5610 NNP denotes BioRad
T19941 5602-5603 -COMMA- denotes ,
T19940 5596-5602 NNP denotes Reader
T19939 5585-5595 NNP denotes Microplate
T19938 5582-5584 CC denotes or
T19937 5574-5581 NNP denotes Austria
T19936 5572-5573 -COMMA- denotes ,
T19935 5564-5572 NNP denotes Salzburg
T19934 5562-5563 -COMMA- denotes ,
T19933 5555-5562 NNP denotes Labotec
T19932 5548-5554 NNP denotes Anthos
T19931 5547-5548 -LRB- denotes (
T19930 5540-5546 NN denotes reader
T19929 5534-5539 NN denotes plate
T19928 5528-5533 NN denotes ELISA
T19927 5525-5527 DT denotes an
T19926 5519-5524 VB denotes using
T19925 5508-5518 VB denotes quantified
T19924 5503-5507 VB denotes were
T19923 5493-5502 NN denotes cytokines
T19922 5489-5492 DT denotes All
T20426 6354-6360 IN denotes before
T20425 6344-6353 VB denotes described
T20424 6341-6343 IN denotes as
T20423 6338-6340 NN denotes GP
T20422 6333-6337 IN denotes with
T20421 6322-6332 NN denotes incubation
T20420 6320-6321 NN denotes h
T20419 6318-6319 CD denotes 1
T20418 6312-6317 IN denotes after
T20417 6301-6311 VB denotes determined
T20416 6296-6300 VB denotes were
T20415 6289-6295 NN denotes shifts
T20414 6284-6288 NN denotes Band
T20413 6280-6282 NN denotes GP
T20412 6275-6279 IN denotes with
T20411 6264-6274 NN denotes incubation
T20410 6261-6263 TO denotes to
T20409 6255-6260 JJ denotes prior
T20408 6253-6254 -RRB- denotes )
T20407 6240-6253 NNP denotes Biotechnology
T20406 6235-6239 NNP denotes Cruz
T20405 6229-6234 NNP denotes Santa
T20404 6228-6229 -LRB- denotes (
T20403 6214-6227 NN denotes cycloheximide
T20402 6208-6213 NN denotes μg/ml
T20401 6205-6207 CD denotes 10
T20400 6203-6204 -COLON- denotes ;
T20399 6202-6203 -RRB- denotes )
T20398 6195-6202 NNP denotes Germany
T20397 6193-6194 -COMMA- denotes ,
T20396 6188-6193 NNP denotes Soden
T20395 6184-6187 NNP denotes Bad
T20394 6182-6183 -COMMA- denotes ,
T20393 6171-6182 NNP denotes Biosciences
T20392 6154-6170 NNP denotes Calbiochem-Merck
T20391 6153-6154 -LRB- denotes (
T20390 6151-6152 NN denotes A
T20389 6139-6150 NN denotes cyclosporin
T20388 6133-6138 NN denotes ng/ml
T20387 6129-6132 CD denotes 400
T20386 6127-6128 -COLON- denotes ;
T20385 6126-6127 -RRB- denotes )
T20384 6119-6126 NNP denotes Germany
T20383 6117-6118 -COMMA- denotes ,
T20382 6110-6117 NNP denotes Hamburg
T20381 6108-6109 -COMMA- denotes ,
T20380 6102-6108 NNP denotes Biomol
T20379 6101-6102 -LRB- denotes (
T20378 6099-6100 -COMMA- denotes ,
T20377 6098-6099 -RRB- denotes )
T20376 6051-6098 NN denotes caffeic-3,4-dihydroxycinnamic-acid-phenyl-ester
T20375 6050-6051 -LRB- denotes (
T20374 6045-6049 NN denotes CAPE
T20373 6042-6044 IN denotes of
T20372 6036-6041 NN denotes μg/ml
T20371 6033-6035 CD denotes 50
T19921 5478-5487 NN denotes standards
T19920 5474-5477 DT denotes the
T19919 5470-5473 IN denotes for
T19918 5465-5469 VB denotes used
T19917 5460-5464 DT denotes that
T19916 5457-5459 IN denotes as
T19915 5450-5456 NN denotes buffer
T19914 5445-5449 JJ denotes same
T19913 5441-5444 DT denotes the
T19912 5438-5440 IN denotes in
T19911 5430-5437 VB denotes diluted
T19910 5425-5429 VB denotes were
T19909 5417-5424 NN denotes Samples
T19908 5414-5415 -RRB- denotes ]
T19907 5412-5414 CD denotes 46
T19906 5411-5412 -LRB- denotes [
T19905 5401-5410 VB denotes described
T19904 5390-5400 RB denotes previously
T19903 5387-5389 IN denotes as
T19902 5382-5386 VB denotes done
T19901 5378-5381 VB denotes was
T19900 5373-5377 NN denotes IL-1
T19899 5370-5372 IN denotes of
T19898 5358-5369 NN denotes bioactivity
T19897 5354-5357 DT denotes the
T19896 5350-5353 IN denotes for
T19824 4995-4998 CC denotes and
T19823 4992-4994 CD denotes 36
T19822 4990-4991 -COMMA- denotes ,
T19821 4988-4990 CD denotes 30
T19820 4986-4987 -COMMA- denotes ,
T19819 4984-4986 CD denotes 24
T19818 4982-4983 -COMMA- denotes ,
T19817 4980-4982 CD denotes 18
T19816 4978-4979 -COMMA- denotes ,
T19815 4976-4978 CD denotes 12
T19814 4974-4975 -COMMA- denotes ,
T19813 4973-4974 CD denotes 9
T19812 4971-4972 -COMMA- denotes ,
T19811 4970-4971 CD denotes 6
T19810 4968-4969 -COMMA- denotes ,
T19809 4967-4968 CD denotes 3
T19808 4957-4966 VB denotes harvested
T19807 4952-4956 VB denotes were
T19806 4943-4951 NN denotes cultures
T19805 4938-4942 NN denotes cell
T19804 4935-4937 IN denotes of
T19803 4922-4934 NN denotes Supernatants
T19802 4909-4921 NN denotes measurements
T19801 4900-4908 NN denotes Cytokine
T19895 5340-5349 NN denotes indicator
T19894 5337-5339 DT denotes an
T19893 5334-5336 IN denotes as
T19892 5329-5333 NN denotes IL-2
T19891 5326-5328 IN denotes of
T19890 5311-5325 NN denotes Quantification
T19889 5308-5309 -RRB- denotes ]
T19888 5306-5308 CD denotes 57
T19887 5305-5306 -LRB- denotes [
T19886 5295-5304 VB denotes described
T19885 5284-5294 RB denotes previously
T19884 5281-5283 IN denotes as
T19883 5275-5280 NN denotes ELISA
T19882 5272-5274 DT denotes an
T19881 5266-5271 VB denotes using
T19880 5264-5265 CD denotes 1
T19879 5259-5263 NN denotes TGFβ
T19878 5255-5258 CC denotes and
T19877 5253-5254 -COMMA- denotes ,
T19876 5252-5253 -RRB- denotes )
T19875 5245-5252 NNP denotes Germany
T19874 5243-5244 -COMMA- denotes ,
T19873 5222-5243 NNP denotes Wiesbaden-Nordenstadt
T19872 5221-5222 -LRB- denotes (
T19871 5213-5220 NNP denotes Systems
T19870 5211-5212 NNP denotes D
T19869 5210-5211 CC denotes &
T19868 5209-5210 NNP denotes R
T19867 5204-5208 IN denotes from
T19866 5198-5203 NN denotes ELISA
T19865 5195-5197 DT denotes an
T19864 5192-5194 IN denotes by
T19863 5187-5191 NN denotes IL-8
T19862 5183-5186 CC denotes and
T19861 5176-5182 NN denotes IL-1RA
T19860 5174-5175 -COMMA- denotes ,
T19859 5173-5174 -RRB- denotes )
T19858 5166-5173 NNP denotes Austria
T19857 5164-5165 -COMMA- denotes ,
T19856 5158-5164 NNP denotes Vienna
T19855 5157-5158 -LRB- denotes (
T19854 5149-5156 NNP denotes Systems
T19853 5142-5148 NNP denotes Bender
T19852 5137-5141 IN denotes from
T19851 5131-5136 NN denotes ELISA
T19850 5128-5130 DT denotes an
T19849 5122-5127 VB denotes using
T19848 5111-5121 VB denotes determined
T19847 5106-5110 VB denotes were
T19846 5101-5105 NN denotes IL-6
T19845 5097-5100 CC denotes and
T19844 5092-5096 NN denotes IL-1
T19843 5090-5091 -COMMA- denotes ,
T19842 5086-5090 NN denotes TNFα
T19841 5084-5085 -COMMA- denotes ,
T19840 5080-5084 NN denotes IFNγ
T19839 5074-5078 RB denotes once
T19838 5069-5073 RB denotes only
T19837 5062-5068 VB denotes thawed
T19836 5058-5061 CC denotes and
T19835 5053-5057 VB denotes used
T19834 5047-5052 IN denotes until
T19833 5044-5046 NN denotes °C
T19832 5040-5043 CD denotes -80
T19831 5037-5039 IN denotes at
T19830 5030-5036 VB denotes stored
T19829 5026-5029 CC denotes and
T19828 5014-5025 NN denotes stimulation
T19827 5008-5013 IN denotes after
T19826 5002-5007 NN denotes hours
T19825 4999-5001 CD denotes 48
T19361 4896-4897 -RRB- denotes )
T19360 4893-4896 NNP denotes USA
T19359 4891-4892 -COMMA- denotes ,
T19358 4889-4891 NNP denotes CA
T19357 4887-4888 -COMMA- denotes ,
T19356 4877-4887 NNP denotes Burlingame
T19355 4872-4876 NNP denotes Inc.
T19354 4859-4871 NNP denotes Laboratories
T18963 3618-3625 NN denotes binding
T18962 3609-3617 JJ denotes specific
T18961 3604-3608 IN denotes with
T18960 3596-3603 VB denotes compete
T18959 3592-3595 RB denotes not
T18958 3588-3591 VB denotes did
T18957 3582-3587 WDT denotes which
T18956 3580-3581 -COMMA- denotes ,
T18955 3576-3580 VB denotes used
T18954 3572-3575 VB denotes was
T18953 3570-3571 -RRB- denotes )
T18952 3569-3570 CD denotes 1
T18951 3563-3568 JJ denotes Table
T18950 3561-3562 -COMMA- denotes ,
T18949 3558-3561 NN denotes DNA
T18948 3548-3557 JJ denotes unrelated
T18947 3547-3548 -LRB- denotes (
T18946 3543-3546 NN denotes DNA
T18945 3537-3542 NN denotes Oct-1
T18944 3534-3536 IN denotes of
T18943 3527-3533 NN denotes excess
T18942 3521-3526 JJ denotes molar
T18941 3513-3520 JJ denotes 30-fold
T18940 3511-3512 DT denotes a
T18939 3509-3510 -COMMA- denotes ,
T18938 3500-3509 NN denotes reactions
T18937 3487-3499 JJ denotes non-specific
T18936 3479-3486 VB denotes exclude
T18935 3476-3478 TO denotes To
T18934 3473-3474 -RRB- denotes )
T18933 3468-3473 VB denotes shown
T18932 3464-3467 RB denotes not
T18931 3459-3463 NN denotes data
T18930 3458-3459 -LRB- denotes (
T18929 3448-3457 VB denotes performed
T18928 3443-3447 VB denotes were
T18927 3434-3442 NN denotes antibody
T18926 3427-3433 NN denotes p65/50
T18925 3418-3426 JJ denotes combined
T18924 3416-3417 DT denotes a
T18923 3411-3415 IN denotes with
T18922 3406-3410 NN denotes NFκB
T18921 3402-3405 IN denotes for
T18920 3395-3401 NN denotes assays
T18919 3384-3394 NN denotes supershift
T18918 3382-3383 -COMMA- denotes ,
T18917 3375-3382 NN denotes binding
T18916 3372-3374 IN denotes of
T18915 3360-3371 NN denotes specificity
T18914 3356-3359 IN denotes For
T18913 3346-3354 NN denotes extracts
T18912 3338-3345 JJ denotes nuclear
T18911 3334-3337 DT denotes the
T18910 3331-3333 TO denotes to
T18909 3325-3330 VB denotes added
T18908 3321-3324 VB denotes was
T18907 3314-3320 NN denotes oligos
T18906 3304-3313 JJ denotes unlabeled
T18905 3301-3303 IN denotes of
T18904 3299-3300 -RRB- denotes )
T18903 3294-3299 NN denotes 5–50×
T18902 3293-3294 -LRB- denotes (
T18901 3286-3292 JJ denotes excess
T18900 3283-3285 DT denotes an
T18899 3281-3282 -COMMA- denotes ,
T18898 3270-3281 NN denotes experiments
T18897 3265-3269 JJ denotes such
T18896 3261-3264 IN denotes For
T18895 3251-3259 NN denotes proteins
T18894 3243-3250 JJ denotes nuclear
T18893 3240-3242 TO denotes to
T18892 3232-3239 NN denotes binding
T18891 3219-3231 JJ denotes non-specific
T18890 3211-3218 VB denotes prevent
T18889 3208-3210 TO denotes to
T18888 3202-3207 NN denotes order
T18887 3199-3201 IN denotes in
T18886 3195-3198 RP denotes out
T18885 3187-3194 VB denotes carried
T18884 3182-3186 VB denotes were
T18883 3180-3181 -RRB- denotes )
T18882 3175-3180 IN denotes above
T18881 3171-3174 VB denotes see
T18880 3170-3171 -LRB- denotes (
T18879 3164-3169 NN denotes sites
T18878 3156-3163 NN denotes binding
T19353 4852-4858 NNP denotes Vector
T19352 4851-4852 -LRB- denotes (
T19351 4847-4850 NN denotes kit
T19350 4838-4846 NN denotes staining
T19349 4826-4837 NNP denotes Vectastain®
T19348 4822-4825 DT denotes the
T19347 4816-4821 VB denotes using
T19346 4806-4815 VB denotes developed
T19345 4802-4805 VB denotes was
T19344 4797-4801 NN denotes blot
T19343 4793-4796 DT denotes The
T19342 4790-4791 -RRB- denotes )
T19341 4787-4790 NN denotes BSA
T19340 4785-4786 NN denotes %
T19339 4780-4785 NN denotes PBS/1
T19338 4777-4779 IN denotes in
T19337 4773-4776 CD denotes 500
T19336 4772-4773 -COLON- denotes :
T19335 4771-4772 CD denotes 1
T19334 4770-4771 -LRB- denotes (
T19333 4761-4769 NN denotes antibody
T19332 4751-4760 JJ denotes secondary
T19331 4728-4750 JJ denotes phosphatase-conjugated
T19330 4719-4727 NN denotes alkaline
T19329 4715-4718 DT denotes the
T19328 4710-4714 IN denotes with
T19327 4700-4709 VB denotes incubated
T19326 4696-4699 CC denotes and
T19325 4690-4695 RB denotes again
T19324 4683-4689 VB denotes washed
T19323 4681-4682 -COMMA- denotes ,
T19322 4678-4681 NN denotes BSA
T19321 4676-4677 NN denotes %
T19320 4671-4676 NN denotes PBS/1
T19319 4668-4670 IN denotes in
T18877 3147-3155 NN denotes promoter
T18876 3138-3146 NN denotes cytokine
T18875 3134-3137 DT denotes the
T18874 3131-3133 IN denotes of
T18873 3124-3130 NN denotes oligos
T18872 3116-3123 VB denotes mutated
T18871 3106-3115 JJ denotes unlabeled
T18870 3102-3105 CC denotes and
T18869 3094-3101 VB denotes labeled
T18868 3089-3093 IN denotes with
T18867 3077-3088 NN denotes experiments
T18866 3065-3076 NN denotes Competition
T18865 3055-3063 NN denotes controls
T18864 3042-3054 JJ denotes unstimulated
T18863 3039-3041 IN denotes of
T18862 3033-3038 NN denotes bands
T18861 3030-3032 TO denotes to
T18860 3019-3029 VB denotes normalized
T18859 3015-3018 VB denotes was
T18858 3009-3014 NN denotes bands
T18857 3001-3008 VB denotes shifted
T18856 2998-3000 IN denotes of
T18855 2988-2997 NN denotes intensity
T18854 2984-2987 DT denotes The
T18853 2981-2982 -RRB- denotes ]
T18852 2979-2981 CD denotes 56
T18814 2752-2763 NN denotes Preparation
T18476 2734-2736 POS denotes 's
T18475 2722-2734 NN denotes manufacturer
T18474 2718-2721 DT denotes the
T18473 2715-2717 TO denotes to
T18472 2705-2714 VB denotes according
T18471 2703-2704 -RRB- denotes )
T18470 2694-2703 NNP denotes Pharmacia
T18469 2693-2694 -LRB- denotes (
T18468 2685-2692 NNP denotes Columns
T18467 2679-2684 NNP denotes Micro
T18466 2674-2678 NNP denotes G-50
T18465 2668-2673 NNP denotes Quant
T18464 2662-2667 NNP denotes Probe
T18463 2656-2661 VB denotes using
T18462 2645-2655 NN denotes filtration
T18461 2641-2644 NN denotes gel
T18460 2637-2640 IN denotes via
T18459 2628-2636 VB denotes purified
T18458 2623-2627 VB denotes were
T18457 2616-2622 NN denotes oligos
T18456 2613-2615 NN denotes ds
T18455 2609-2612 DT denotes the
T18454 2607-2608 -COMMA- denotes ,
T18453 2597-2607 RB denotes Afterwards
T18452 2583-2595 RB denotes respectively
T18451 2581-2582 -COMMA- denotes ,
T18450 2573-2581 NN denotes proteins
T18449 2568-2572 NN denotes NFκB
T18448 2564-2567 CC denotes and
T18447 2559-2563 NN denotes NFAT
T18446 2556-2558 IN denotes of
T18445 2548-2555 NN denotes binding
T18444 2544-2547 DT denotes the
T18443 2540-2543 IN denotes for
T18442 2532-2539 JJ denotes crucial
T18441 2529-2531 VB denotes be
T18440 2526-2528 TO denotes to
T18439 2521-2525 VB denotes seem
T18438 2515-2520 WDT denotes which
T18437 2513-2514 -COMMA- denotes ,
T18436 2504-2513 NN denotes sequences
T18435 2499-2503 IN denotes with
T18434 2489-2498 VB denotes interfere
T18433 2486-2488 TO denotes to
T18432 2477-2485 VB denotes reported
T18431 2472-2476 VB denotes been
T18430 2467-2471 VB denotes have
T18429 2465-2466 -RRB- denotes ]
T18428 2463-2465 CD denotes 55
T18427 2462-2463 -LRB- denotes [
T18426 2453-2461 NN denotes promoter
T18425 2448-2452 NN denotes TNFα
T18424 2444-2447 DT denotes the
T18423 2441-2443 IN denotes of
T18422 2432-2440 NN denotes sequence
T18421 2422-2431 NN denotes consensus
T18420 2420-2421 NN denotes κ
T18419 2416-2419 DT denotes the
T18418 2413-2415 IN denotes in
T18417 2409-2412 CC denotes and
T18416 2407-2408 -RRB- denotes ]
T18415 2405-2407 CD denotes 29
T18414 2404-2405 -LRB- denotes [
T18413 2395-2403 NN denotes promoter
T18412 2390-2394 NN denotes IFNγ
T18411 2386-2389 DT denotes the
T18410 2383-2385 IN denotes of
T18409 2378-2382 NN denotes site
T18408 2375-2377 NN denotes P2
T18407 2371-2374 DT denotes the
T18406 2368-2370 IN denotes in
T18851 2978-2979 -LRB- denotes [
T18850 2972-2977 NN denotes setup
T18849 2959-2971 JJ denotes experimental
T18848 2955-2958 PRP-DOLLAR- denotes our
T18847 2951-2954 IN denotes for
T18846 2942-2950 VB denotes modified
T18845 2938-2941 CC denotes and
T18844 2936-2937 -RRB- denotes ]
T18843 2934-2936 CD denotes 27
T18842 2933-2934 -LRB- denotes [
T18841 2929-2932 FW denotes al.
T18840 2926-2928 FW denotes et
T18839 2920-2925 NNP denotes Trede
T18838 2917-2919 IN denotes by
T18837 2907-2916 VB denotes described
T18836 2897-2906 NN denotes technique
T18835 2895-2896 DT denotes a
T18834 2892-2894 TO denotes to
T18833 2882-2891 VB denotes according
T18832 2873-2881 VB denotes prepared
T18831 2868-2872 VB denotes were
T18830 2862-2867 NN denotes PBMCs
T18829 2853-2861 VB denotes purified
T18828 2850-2852 IN denotes of
T18827 2841-2849 NN denotes extracts
T18826 2833-2840 JJ denotes Nuclear
T18825 2831-2832 -RRB- denotes )
T18824 2827-2831 NN denotes EMSA
T18823 2826-2827 -LRB- denotes (
T18822 2819-2825 NN denotes assays
T18821 2813-2818 NN denotes shift
T18820 2804-2812 NN denotes mobility
T18819 2788-2803 JJ denotes electrophoretic
T18818 2784-2787 CC denotes and
T18817 2775-2783 NN denotes extracts
T18816 2767-2774 JJ denotes nuclear
T18815 2764-2766 IN denotes of
T18405 2358-2367 NN denotes Mutations
T18404 2349-2356 NN denotes control
T18403 2338-2348 JJ denotes additional
T18402 2335-2337 DT denotes an
T18401 2332-2334 IN denotes as
T18400 2327-2331 VB denotes used
T18399 2322-2326 VB denotes were
T18398 2315-2321 NN denotes oligos
T18397 2307-2314 VB denotes mutated
T18396 2305-2306 -COMMA- denotes ,
T18395 2299-2305 NN denotes oligos
T18394 2295-2298 DT denotes the
T18393 2292-2294 IN denotes of
T18392 2280-2291 NN denotes specificity
T18391 2272-2279 NN denotes binding
T18390 2268-2271 DT denotes the
T18389 2263-2267 VB denotes test
T18388 2260-2262 TO denotes To
T18387 2246-2258 NN denotes instructions
T18386 2243-2245 POS denotes 's
T18385 2231-2243 NN denotes manufacturer
T18384 2227-2230 DT denotes the
T18383 2224-2226 TO denotes to
T18382 2214-2223 VB denotes according
T18381 2212-2213 -RRB- denotes )
T18380 2205-2212 NNP denotes Germany
T18075 1772-1775 NN denotes LPS
T18074 1768-1771 CC denotes and
T18073 1765-1767 NN denotes GP
T18072 1762-1764 IN denotes of
T18071 1750-1761 NN denotes combination
T18070 1748-1749 DT denotes a
T18069 1743-1747 IN denotes with
T18068 1740-1742 CC denotes or
T18067 1733-1739 NN denotes TSST-1
T18066 1729-1732 CC denotes and
T18065 1726-1728 NN denotes GP
T18064 1723-1725 IN denotes of
T18063 1711-1722 NN denotes combination
T18062 1709-1710 DT denotes a
T18061 1704-1708 IN denotes with
T18060 1689-1703 RB denotes simultaneously
T18059 1676-1688 VB denotes supplemented
T18058 1671-1675 VB denotes were
T18057 1666-1670 NN denotes PBMC
T18056 1664-1665 -COMMA- denotes ,
T18055 1653-1664 NN denotes experiments
T18054 1639-1652 JJ denotes costimulatory
T18053 1636-1638 IN denotes In
T18052 1633-1634 -RRB- denotes )
T18051 1626-1633 NNP denotes Germany
T18050 1624-1625 -COMMA- denotes ,
T18049 1618-1624 NNP denotes Munich
T18048 1616-1617 -COMMA- denotes ,
T18047 1611-1616 NNP denotes Sigma
T18046 1610-1611 -LRB- denotes (
T18045 1607-1609 NN denotes B4
T17986 1354-1355 -RRB- denotes )
T17985 1347-1354 NNP denotes Germany
T17984 1345-1346 -COMMA- denotes ,
T17983 1335-1345 NNP denotes Heidelberg
T17982 1333-1334 -COMMA- denotes ,
T17981 1327-1333 NNP denotes Falcon
T17980 1326-1327 -LRB- denotes (
T17979 1319-1325 NN denotes plates
T17978 1311-1318 NN denotes culture
T17977 1304-1310 JJ denotes 6-well
T17976 1291-1303 JJ denotes pyrogen-free
T17975 1283-1290 JJ denotes sterile
T17974 1280-1282 IN denotes in
T17973 1277-1279 NN denotes ml
T17972 1275-1276 CD denotes 4
T17971 1272-1274 IN denotes of
T17970 1265-1271 NN denotes volume
T17969 1263-1264 DT denotes a
T17968 1260-1262 IN denotes in
T17967 1251-1259 VB denotes cultured
T17966 1246-1250 VB denotes were
T17965 1241-1245 NN denotes PBMC
T17964 1239-1240 -COMMA- denotes ,
T17963 1230-1239 NN denotes cytokines
T17962 1226-1229 CC denotes and
T17961 1218-1225 NN denotes factors
T17960 1204-1217 NN denotes transcription
T17959 1201-1203 IN denotes of
T17958 1191-1200 NN denotes induction
T17957 1187-1190 IN denotes For
T17956 1176-1186 NN denotes conditions
T17955 1164-1175 NN denotes Stimulation
T18379 2203-2204 -COMMA- denotes ,
T18378 2195-2203 NNP denotes Freiburg
T18377 2193-2194 -COMMA- denotes ,
T18376 2190-2193 NNP denotes LKB
T18375 2180-2189 NNP denotes Pharmacia
T18374 2178-2179 -COMMA- denotes ,
T18373 2175-2178 NN denotes Kit
T18372 2171-2174 NN denotes PNK
T18371 2170-2171 -LRB- denotes (
T18370 2166-2169 NN denotes Kit
T18369 2132-2165 NN denotes Ready-To-Go-Polynucleotide-Kinase
T18368 2128-2131 DT denotes the
T18367 2122-2127 VB denotes using
T18366 2120-2121 -RRB- denotes )
T18365 2113-2120 NNP denotes Germany
T18364 2111-2112 -COMMA- denotes ,
T18363 2099-2111 NNP denotes Braunschweig
T18362 2097-2098 -COMMA- denotes ,
T18361 2089-2097 NNP denotes Amersham
T18360 2087-2088 -COMMA- denotes ,
T18359 2080-2087 NN denotes Ci/mmol
T18358 2074-2079 CD denotes 3,000
T18357 2073-2074 -LRB- denotes (
T18356 2063-2072 NN denotes 5'γ-P-ATP
T18355 2058-2062 IN denotes with
T18354 2046-2057 JJ denotes 32P-labeled
T18353 2042-2045 CC denotes and
T18352 2040-2041 -RRB- denotes )
T18351 2033-2040 NNP denotes Germany
T18350 2031-2032 -COMMA- denotes ,
T18349 2025-2031 NNP denotes Berlin
T18348 2023-2024 -COMMA- denotes ,
T18347 2016-2023 NNP denotes Molbiol
T18346 2012-2015 NNP denotes TIB
T18345 2011-2012 -LRB- denotes (
T18344 2009-2010 -COMMA- denotes ,
T18343 2008-2009 -RRB- denotes )
T18342 2007-2008 CD denotes 1
T18341 2001-2006 JJ denotes Table
T18340 1998-2000 IN denotes in
T18339 1986-1997 VB denotes illustrated
T18338 1985-1986 -LRB- denotes (
T18337 1978-1984 NN denotes oligos
T18336 1976-1977 -RRB- denotes )
T18335 1974-1976 NN denotes ss
T18334 1973-1974 -LRB- denotes (
T18333 1964-1972 JJ denotes stranded
T18332 1957-1963 JJ denotes single
T18331 1952-1956 IN denotes from
T18330 1940-1951 VB denotes synthesised
T18329 1935-1939 VB denotes were
T18328 1925-1934 NN denotes promoters
T18327 1920-1924 NN denotes IL-6
T18326 1916-1919 CC denotes and
T18325 1911-1915 NN denotes IFNγ
T18324 1909-1910 -COMMA- denotes ,
T18323 1905-1909 NN denotes IL-8
T18322 1903-1904 -COMMA- denotes ,
T18321 1899-1903 NN denotes TNFα
T18320 1895-1898 DT denotes the
T18319 1890-1894 IN denotes from
T18318 1888-1889 -RRB- denotes )
T18317 1882-1888 NN denotes oligos
T18316 1881-1882 -LRB- denotes (
T18315 1864-1880 NN denotes oligonucleotides
T18314 1862-1863 -RRB- denotes )
T18313 1860-1862 NN denotes ds
T18312 1859-1860 -LRB- denotes (
T18311 1843-1858 JJ denotes double-stranded
T18310 1829-1842 JJ denotes complementary
T18309 1825-1828 DT denotes The
T18308 1808-1824 JJ denotes 5'-32-P-Labeling
T18307 1804-1807 CC denotes and
T18306 1802-1803 -RRB- denotes )
T18305 1796-1802 NN denotes Oligos
T18304 1795-1796 -LRB- denotes (
T17769 1148-1160 NN denotes BioWhittaker
T17768 1147-1148 -LRB- denotes (
T17767 1135-1146 NN denotes L-glutamine
T17766 1132-1134 NN denotes mM
T17765 1128-1131 CD denotes 200
T17764 1126-1127 NN denotes %
T17763 1125-1126 CD denotes 1
T17762 1121-1124 CC denotes and
T17761 1119-1120 -COMMA- denotes ,
T17760 1118-1119 -RRB- denotes )
T17759 1113-1118 NN denotes μg/ml
T17758 1107-1112 CD denotes 10000
T17757 1106-1107 -LRB- denotes (
T17756 1093-1105 NN denotes streptomycin
T17755 1091-1092 NN denotes %
T17754 1090-1091 CD denotes 1
T17753 1088-1089 -COMMA- denotes ,
T17752 1087-1088 -RRB- denotes )
T17751 1083-1087 NN denotes U/ml
T17750 1077-1082 CD denotes 10000
T17749 1076-1077 -LRB- denotes (
T17748 1065-1075 NN denotes penicillin
T17747 1063-1064 NN denotes %
T17746 1062-1063 CD denotes 1
T17745 1060-1061 -COMMA- denotes ,
T17744 1059-1060 -RRB- denotes )
T17743 1052-1059 NNP denotes Germany
T17742 1050-1051 -COMMA- denotes ,
T17741 1041-1050 NNP denotes Karlsruhe
T17740 1039-1040 -COMMA- denotes ,
T17739 1029-1039 NNP denotes Invitrogen
T17738 1027-1028 -COMMA- denotes ,
T17737 1020-1027 NN denotes low-LPS
T17736 1018-1019 -COMMA- denotes ,
T17735 1015-1018 NN denotes FCS
T17734 1014-1015 -LRB- denotes (
T17733 1008-1013 NN denotes serum
T17732 1003-1007 NN denotes calf
T17731 997-1002 JJ denotes fetal
T17730 980-996 JJ denotes heat-inactivated
T17729 978-979 NN denotes %
T17728 976-978 CD denotes 10
T17727 965-975 VB denotes containing
T17726 963-964 -COMMA- denotes ,
T17725 962-963 -RRB- denotes )
T17724 954-962 JJ denotes LPS-free
T17723 952-953 -COMMA- denotes ,
T17722 945-952 NNP denotes Germany
T17721 943-944 -COMMA- denotes ,
T17720 933-943 NNP denotes Heidelberg
T17719 931-932 -COMMA- denotes ,
T17718 919-931 NNP denotes BioWhittaker
T17717 918-919 -LRB- denotes (
T17716 911-917 NN denotes medium
T17715 906-910 CD denotes 1640
T17714 901-905 NN denotes RPMI
T17713 898-900 IN denotes in
T17712 896-897 -RRB- denotes )
T17711 887-896 NN denotes cytometry
T17710 882-886 NN denotes flow
T17709 878-881 IN denotes for
T17708 877-878 -LRB- denotes (
T17707 868-876 NN denotes cells/ml
T17706 864-867 CD denotes 106
T17705 862-863 CC denotes ×
T17704 860-861 CD denotes 1
T17703 857-859 CC denotes or
T17702 855-856 -RRB- denotes )
T17701 849-855 NN denotes shifts
T17700 845-848 NN denotes gel
T17699 841-844 IN denotes for
T17698 840-841 -LRB- denotes (
T17697 833-839 NN denotes 106/ml
T17696 831-832 CC denotes ×
T17695 829-830 CD denotes 5
T17694 826-828 CC denotes or
T17693 824-825 -RRB- denotes )
T17692 812-824 NN denotes measurements
T17691 806-811 NN denotes ELISA
T17690 802-805 IN denotes for
T17689 801-802 -LRB- denotes (
T17688 792-800 NN denotes cells/ml
T17687 788-791 CD denotes 106
T17686 786-787 -COLON- denotes ×
T17685 784-785 CD denotes 3
T17684 781-783 IN denotes of
T17683 767-780 NN denotes concentration
T17682 761-766 JJ denotes final
T17681 759-760 DT denotes a
T17680 756-758 IN denotes at
T17679 747-755 VB denotes cultured
T17678 742-746 VB denotes were
T17677 737-741 NN denotes PBMC
T17676 735-736 -COMMA- denotes ,
T17675 726-735 NN denotes isolation
T17674 716-725 VB denotes Following
T17673 713-714 -RRB- denotes ]
T17672 708-713 CD denotes 54,55
T17671 707-708 -LRB- denotes [
T17670 698-706 NN denotes gradient
T17669 696-697 -RRB- denotes )
T17668 689-696 NNP denotes Germany
T17667 687-688 -COMMA- denotes ,
T17666 681-687 NNP denotes Berlin
T17665 679-680 -COMMA- denotes ,
T17664 671-679 NNP denotes Biochrom
T17663 670-671 -LRB- denotes (
T17662 655-669 JJ denotes Ficoll-Hypaque
T17661 653-654 DT denotes a
T17660 648-652 IN denotes over
T17659 638-647 VB denotes separated
T17658 633-637 VB denotes were
T17657 627-632 NN denotes coats
T17656 621-626 NN denotes buffy
T17655 617-620 DT denotes The
T17654 608-616 NNP denotes Luebeck.
T17653 605-607 IN denotes of
T17652 594-604 NNP denotes University
T17651 590-593 DT denotes the
T17650 587-589 IN denotes of
T17649 577-586 NN denotes committee
T17648 570-576 NN denotes ethics
T17647 566-569 DT denotes the
T17646 563-565 IN denotes of
T17645 554-562 NN denotes approval
T17644 550-553 DT denotes the
T17643 545-549 IN denotes with
T17642 541-544 CC denotes and
T17641 529-540 NN denotes declaration
T17640 520-528 NNP denotes Helsinki
T17639 516-519 DT denotes the
T17638 511-515 IN denotes with
T17637 500-510 NN denotes compliance
T17636 497-499 IN denotes in
T17635 490-496 NN denotes donors
T17634 484-489 NN denotes blood
T17633 476-483 JJ denotes healthy
T17632 471-475 IN denotes from
T17631 462-470 VB denotes obtained
T17630 457-461 VB denotes were
T17629 451-456 NN denotes coats
T17628 445-450 JJ denotes Buffy
T17627 440-444 NN denotes PBMC
T17626 434-439 JJ denotes human
T17625 431-433 IN denotes of
T17624 421-430 NN denotes Isolation
T18044 1606-1607 -COLON- denotes :
T18043 1602-1606 CD denotes 0111
T18042 1593-1601 NN denotes serotype
T18041 1588-1592 FW denotes coli
T18040 1576-1587 FW denotes Escherichia
T18039 1571-1575 IN denotes from
T18038 1567-1570 NN denotes LPS
T18037 1557-1566 JJ denotes wild-type
T18036 1551-1556 NN denotes cells
T18035 1544-1550 NN denotes ng/106
T18034 1540-1543 CD denotes 250
T18033 1535-1539 IN denotes with
T18032 1532-1534 CC denotes or
T18031 1530-1531 -RRB- denotes )
T18030 1527-1530 NNP denotes USA
T18029 1525-1526 -COMMA- denotes ,
T18028 1523-1525 NNP denotes FL
T18027 1521-1522 -COMMA- denotes ,
T18026 1513-1521 NNP denotes Sarasota
T18025 1511-1512 -COMMA- denotes ,
T18024 1499-1511 NNP denotes Technologies
T18023 1493-1498 NNP denotes Toxin
T18022 1492-1493 -LRB- denotes (
T18021 1485-1491 NN denotes TSST-1
T18020 1479-1484 NN denotes cells
T18019 1472-1478 NN denotes ng/106
T18018 1468-1471 CD denotes 250
T18017 1463-1467 IN denotes with
T18016 1461-1462 -COMMA- denotes ,
T18015 1459-1461 NN denotes GP
T18014 1456-1458 IN denotes of
T18013 1450-1455 NN denotes cells
T18012 1446-1449 CD denotes 106
T18011 1442-1445 IN denotes per
T18010 1439-1441 NN denotes μg
T18009 1435-1438 CD denotes 100
T18008 1432-1434 CC denotes or
T18007 1430-1431 CD denotes 1
T18006 1425-1429 IN denotes with
T18005 1423-1424 -RRB- denotes )
T18004 1418-1423 NN denotes ELISA
T18003 1417-1418 -LRB- denotes (
T18002 1415-1416 NN denotes h
T18001 1412-1414 CD denotes 48
T18000 1409-1411 TO denotes to
T17999 1407-1408 NN denotes h
T17998 1405-1406 CD denotes 0
T17997 1400-1404 IN denotes from
T17996 1397-1399 CC denotes or
T17995 1395-1396 -RRB- denotes )
T17994 1391-1395 NN denotes EMSA
T17993 1390-1391 -LRB- denotes (
T17992 1388-1389 NN denotes h
T17991 1386-1387 CD denotes 1
T17990 1382-1385 IN denotes for
T17989 1371-1381 VB denotes stimulated
T17988 1366-1370 VB denotes were
T17987 1357-1365 NN denotes Cultures
T17535 403-418 NN denotes β-1→3-D-glucans
T17534 400-402 TO denotes to
T17533 392-399 VB denotes respond
T17532 388-391 RB denotes not
T17531 383-387 VB denotes does
T17530 379-382 CC denotes but
T17529 369-378 NN denotes endotoxin
T17528 365-368 IN denotes for
T17527 356-364 JJ denotes specific
T17526 353-355 VB denotes is
T17525 347-352 WDT denotes which
T17524 345-346 -COMMA- denotes ,
T17523 344-345 -RRB- denotes )
T17522 339-344 NNP denotes Japan
T17521 337-338 -COMMA- denotes ,
T17520 332-337 NNP denotes Tokyo
T17519 330-331 -COMMA- denotes ,
T17518 321-330 NNP denotes Seigakaku
T17517 320-321 -LRB- denotes (
T17516 314-319 NN denotes assay
T17515 304-313 NN denotes Endospecy
T17514 300-303 DT denotes the
T17513 295-299 IN denotes with
T17512 281-294 NN denotes contamination
T17511 271-280 NN denotes endotoxin
T17510 267-270 IN denotes for
T17509 258-266 VB denotes screened
T17508 254-257 CC denotes and
T17507 252-253 -RRB- denotes )
T17506 250-252 NN denotes μm
T17505 246-249 CD denotes 0.2
T17504 245-246 -LRB- denotes (
T17503 227-244 JJ denotes filter-sterilized
T17502 225-226 -COMMA- denotes ,
T17501 220-225 NN denotes media
T17500 212-219 JJ denotes aqueous
T17499 209-211 IN denotes in
T17498 199-208 VB denotes dissolved
T17497 195-198 VB denotes was
T17496 192-194 NN denotes GP
T17495 189-190 -RRB- denotes ]
T17494 184-189 CD denotes 20,26
T17493 183-184 -LRB- denotes [
T17492 172-182 RB denotes previously
T17491 163-171 VB denotes reported
T17490 160-162 IN denotes as
T17489 149-159 VB denotes determined
T17488 144-148 VB denotes were
T17487 141-143 NN denotes GP
T17486 138-140 IN denotes of
T17485 122-137 NN denotes characteristics
T17484 106-121 JJ denotes physicochemical
T17483 102-105 DT denotes The
T17482 99-100 -RRB- denotes ]
T17481 97-99 CD denotes 20
T17480 96-97 -LRB- denotes [
T17479 89-95 IN denotes before
T17478 84-88 VB denotes done
T17477 81-83 IN denotes as
T17476 72-80 VB denotes prepared
T17475 68-71 VB denotes was
T17474 66-67 -RRB- denotes )
T17473 64-66 NN denotes GP
T17472 63-64 -LRB- denotes (
T17471 48-62 NN denotes β-1→3-D-glucan
T17470 33-47 VB denotes phosphorylated
T17469 25-32 JJ denotes soluble
T17468 19-24 RB denotes Water
T17467 9-18 NN denotes Materials
T17770 1160-1161 -RRB- denotes )
T18477 2737-2749 NN denotes instructions
T19306 4592-4595 DT denotes the
T19305 4590-4591 -COMMA- denotes ,
T19304 4589-4590 -RRB- denotes )
T19303 4582-4589 NNP denotes Germany
T19302 4580-4581 -COMMA- denotes ,
T19301 4569-4580 NNP denotes Deisenhofen
T19300 4567-4568 -COMMA- denotes ,
T19299 4562-4567 NNP denotes Fluka
T19298 4561-4562 -LRB- denotes (
T19297 4557-4560 NN denotes BSA
T19296 4555-4556 NN denotes %
T19295 4550-4555 NN denotes PBS/3
T19294 4545-4549 IN denotes with
T19293 4536-4544 VB denotes blocking
T19292 4530-4535 IN denotes After
T19291 4527-4528 -RRB- denotes )
T19290 4520-4527 NN denotes Bio-Rad
T19289 4519-4520 -LRB- denotes (
T19288 4510-4518 NN denotes membrane
T19287 4495-4509 NN denotes nitrocellulose
T19286 4493-4494 DT denotes a
T19285 4488-4492 IN denotes onto
T19284 4480-4487 VB denotes blotted
T19283 4475-4479 VB denotes were
T19282 4466-4474 NN denotes proteins
T19281 4458-4465 JJ denotes nuclear
T19280 4454-4457 DT denotes The
T19279 3641-3645 NN denotes blot
T19278 3639-3640 -RRB- denotes )
T19277 3636-3639 NN denotes Dot
T19276 3635-3636 -LRB- denotes (
T19275 3628-3634 NN denotes Immuno
T19318 4663-4667 CD denotes 2000
T19317 4662-4663 -COLON- denotes :
T19316 4661-4662 CD denotes 1
T19315 4653-4660 VB denotes diluted
T19314 4642-4652 NN denotes antibodies
T19313 4634-4641 JJ denotes primary
T19312 4630-4633 DT denotes the
T19311 4625-4629 IN denotes with
T19310 4615-4624 RB denotes overnight
T19309 4605-4614 VB denotes incubated
T19308 4601-4604 VB denotes was
T19307 4596-4600 NN denotes blot
T18303 1778-1794 NN denotes Oligonucleotides
R11765 T17471 T17469 arg1Of β-1→3-D-glucan,soluble
R11766 T17471 T17470 arg2Of β-1→3-D-glucan,phosphorylated
R11767 T17471 T17472 arg1Of β-1→3-D-glucan,(
R11768 T17471 T17475 arg1Of β-1→3-D-glucan,was
R11769 T17471 T17476 arg2Of β-1→3-D-glucan,prepared
R11770 T17473 T17472 arg2Of GP,(
R11771 T17474 T17472 arg3Of ),(
R11772 T17476 T17468 arg1Of prepared,Water
R11773 T17476 T17475 arg2Of prepared,was
R11774 T17476 T17477 arg1Of prepared,as
R11775 T17478 T17477 arg2Of done,as
R11776 T17478 T17479 arg1Of done,before
R11777 T17481 T17479 arg2Of 20,before
R11778 T17481 T17480 arg2Of 20,[
R11779 T17482 T17480 arg3Of ],[
R11780 T17485 T17483 arg1Of characteristics,The
R11781 T17485 T17484 arg1Of characteristics,physicochemical
R11782 T17485 T17486 arg1Of characteristics,of
R11783 T17485 T17488 arg1Of characteristics,were
R11784 T17485 T17489 arg2Of characteristics,determined
R11785 T17487 T17486 arg2Of GP,of
R11786 T17489 T17488 arg2Of determined,were
R11787 T17489 T17490 arg1Of determined,as
R11788 T17489 T17493 arg1Of determined,[
R11789 T17491 T17490 arg2Of reported,as
R11790 T17491 T17492 arg1Of reported,previously
R11791 T17494 T17493 arg2Of "20,26",[
R11792 T17495 T17493 arg3Of ],[
R11793 T17496 T17497 arg1Of GP,was
R11794 T17496 T17498 arg2Of GP,dissolved
R11795 T17496 T17509 arg2Of GP,screened
R11796 T17498 T17499 arg1Of dissolved,in
R11797 T17498 T17508 arg1Of dissolved,and
R11798 T17501 T17499 arg2Of media,in
R11799 T17501 T17500 arg1Of media,aqueous
R11800 T17501 T17502 arg1Of media,","
R11801 T17503 T17502 arg2Of filter-sterilized,","
R11802 T17503 T17504 arg1Of filter-sterilized,(
R11803 T17506 T17504 arg2Of μm,(
R11804 T17506 T17505 arg1Of μm,0.2
R11805 T17507 T17504 arg3Of ),(
R11806 T17508 T17497 arg2Of and,was
R11807 T17509 T17508 arg2Of screened,and
R11808 T17509 T17510 arg1Of screened,for
R11809 T17509 T17513 arg1Of screened,with
R11810 T17512 T17510 arg2Of contamination,for
R11811 T17512 T17511 arg1Of contamination,endotoxin
R11812 T17516 T17513 arg2Of assay,with
R11813 T17516 T17514 arg1Of assay,the
R11814 T17516 T17515 arg1Of assay,Endospecy
R11815 T17516 T17517 arg1Of assay,(
R11816 T17516 T17524 arg1Of assay,","
R11817 T17516 T17525 arg1Of assay,which
R11818 T17516 T17526 arg1Of assay,is
R11819 T17516 T17527 arg1Of assay,specific
R11820 T17516 T17531 arg1Of assay,does
R11821 T17516 T17533 arg1Of assay,respond
R11822 T17518 T17519 arg1Of Seigakaku,","
R11823 T17519 T17517 arg2Of ",",(
R11824 T17519 T17521 arg1Of ",",","
R11825 T17520 T17519 arg2Of Tokyo,","
R11826 T17522 T17521 arg2Of Japan,","
R11827 T17523 T17517 arg3Of ),(
R11828 T17526 T17530 arg1Of is,but
R11829 T17527 T17526 arg2Of specific,is
R11830 T17527 T17528 arg1Of specific,for
R11831 T17529 T17528 arg2Of endotoxin,for
R11832 T17533 T17530 arg2Of respond,but
R11833 T17533 T17531 arg2Of respond,does
R11834 T17533 T17532 arg1Of respond,not
R11835 T17533 T17534 arg1Of respond,to
R11836 T17535 T17534 arg2Of β-1→3-D-glucans,to
R11913 T17624 T17625 arg1Of Isolation,of
R11914 T17627 T17625 arg2Of PBMC,of
R11915 T17627 T17626 arg1Of PBMC,human
R11916 T17629 T17628 arg1Of coats,Buffy
R11917 T17629 T17630 arg1Of coats,were
R11918 T17629 T17631 arg2Of coats,obtained
R11919 T17631 T17630 arg2Of obtained,were
R11920 T17631 T17632 arg1Of obtained,from
R11921 T17631 T17643 arg1Of obtained,with
R11922 T17632 T17642 arg1Of from,and
R11923 T17635 T17632 arg2Of donors,from
R11924 T17635 T17633 arg1Of donors,healthy
R11925 T17635 T17634 arg1Of donors,blood
R11926 T17635 T17636 arg1Of donors,in
R11927 T17637 T17636 arg2Of compliance,in
R11928 T17637 T17638 arg1Of compliance,with
R11929 T17641 T17638 arg2Of declaration,with
R11930 T17641 T17639 arg1Of declaration,the
R11931 T17641 T17640 arg1Of declaration,Helsinki
R11932 T17643 T17642 arg2Of with,and
R11933 T17645 T17643 arg2Of approval,with
R11934 T17645 T17644 arg1Of approval,the
R11935 T17645 T17646 arg1Of approval,of
R11936 T17649 T17646 arg2Of committee,of
R11937 T17649 T17647 arg1Of committee,the
R11938 T17649 T17648 arg1Of committee,ethics
R11939 T17649 T17650 arg1Of committee,of
R11940 T17649 T17658 modOf committee,were
R11941 T17652 T17650 arg2Of University,of
R11942 T17652 T17651 arg1Of University,the
R11943 T17652 T17653 arg1Of University,of
R11944 T17654 T17653 arg2Of Luebeck.,of
R11945 T17657 T17655 arg1Of coats,The
R11946 T17657 T17656 arg1Of coats,buffy
R11947 T17657 T17658 arg1Of coats,were
R11948 T17657 T17659 arg2Of coats,separated
R11949 T17659 T17658 arg2Of separated,were
R11950 T17659 T17660 arg1Of separated,over
R11951 T17659 T17671 arg1Of separated,[
R11952 T17662 T17663 arg1Of Ficoll-Hypaque,(
R11953 T17664 T17665 arg1Of Biochrom,","
R11954 T17665 T17663 arg2Of ",",(
R11955 T17665 T17667 arg1Of ",",","
R11956 T17666 T17665 arg2Of Berlin,","
R11957 T17668 T17667 arg2Of Germany,","
R11958 T17669 T17663 arg3Of ),(
R11959 T17670 T17660 arg2Of gradient,over
R11960 T17670 T17661 arg1Of gradient,a
R11961 T17670 T17662 arg1Of gradient,Ficoll-Hypaque
R11962 T17672 T17671 arg2Of "54,55",[
R11963 T17673 T17671 arg3Of ],[
R11964 T17675 T17674 arg2Of isolation,Following
R11965 T17677 T17678 arg1Of PBMC,were
R11966 T17677 T17679 arg2Of PBMC,cultured
R11967 T17679 T17674 arg1Of cultured,Following
R11968 T17679 T17676 arg1Of cultured,","
R11969 T17679 T17678 arg2Of cultured,were
R11970 T17679 T17680 arg1Of cultured,at
R11971 T17683 T17680 arg2Of concentration,at
R11972 T17683 T17681 arg1Of concentration,a
R11973 T17683 T17682 arg1Of concentration,final
R11974 T17683 T17684 arg1Of concentration,of
R11975 T17685 T17684 arg2Of 3,of
R11976 T17688 T17687 arg1Of cells/ml,106
R11977 T17688 T17689 arg1Of cells/ml,(
R11978 T17688 T17694 arg1Of cells/ml,or
R11979 T17690 T17689 arg2Of for,(
R11980 T17692 T17690 arg2Of measurements,for
R11981 T17692 T17691 arg1Of measurements,ELISA
R11982 T17693 T17689 arg3Of ),(
R11983 T17694 T17703 arg1Of or,or
R11984 T17695 T17696 arg1Of 5,×
R11985 T17696 T17694 arg2Of ×,or
R11986 T17696 T17698 arg1Of ×,(
R11987 T17697 T17696 arg2Of 106/ml,×
R11988 T17699 T17698 arg2Of for,(
R11989 T17701 T17699 arg2Of shifts,for
R11990 T17701 T17700 arg1Of shifts,gel
R11991 T17702 T17698 arg3Of ),(
R11992 T17703 T17680 arg3Of or,at
R11993 T17703 T17686 arg1Of or,×
R11994 T17704 T17705 arg1Of 1,×
R11995 T17706 T17705 arg2Of 106,×
R11996 T17707 T17704 arg1Of cells/ml,1
R11997 T17707 T17706 arg1Of cells/ml,106
R11998 T17707 T17708 arg1Of cells/ml,(
R11999 T17707 T17713 arg1Of cells/ml,in
R12000 T17707 T17726 arg1Of cells/ml,","
R12001 T17707 T17727 arg1Of cells/ml,containing
R12002 T17707 T17734 arg1Of cells/ml,(
R12003 T17707 T17745 arg1Of cells/ml,","
R12004 T17709 T17708 arg2Of for,(
R12005 T17711 T17709 arg2Of cytometry,for
R12006 T17711 T17710 arg1Of cytometry,flow
R12007 T17712 T17708 arg3Of ),(
R12008 T17716 T17713 arg2Of medium,in
R12009 T17716 T17714 arg1Of medium,RPMI
R12010 T17716 T17715 arg1Of medium,1640
R12011 T17716 T17717 arg1Of medium,(
R12012 T17718 T17719 arg1Of BioWhittaker,","
R12013 T17719 T17721 arg1Of ",",","
R12014 T17720 T17719 arg2Of Heidelberg,","
R12015 T17721 T17717 arg2Of ",",(
R12016 T17721 T17723 arg1Of ",",","
R12017 T17721 T17724 arg1Of ",",LPS-free
R12018 T17722 T17721 arg2Of Germany,","
R12019 T17725 T17717 arg3Of ),(
R12020 T17728 T17729 arg1Of 10,%
R12021 T17733 T17727 arg2Of serum,containing
R12022 T17733 T17728 arg1Of serum,10
R12023 T17733 T17730 arg1Of serum,heat-inactivated
R12024 T17733 T17731 arg1Of serum,fetal
R12025 T17733 T17732 arg1Of serum,calf
R12026 T17735 T17736 arg1Of FCS,","
R12027 T17735 T17738 arg1Of FCS,","
R12028 T17735 T17740 arg1Of FCS,","
R12029 T17737 T17736 arg2Of low-LPS,","
R12030 T17739 T17738 arg2Of Invitrogen,","
R12031 T17740 T17734 arg2Of ",",(
R12032 T17741 T17740 arg2Of Karlsruhe,","
R12033 T17741 T17742 arg1Of Karlsruhe,","
R12034 T17743 T17742 arg2Of Germany,","
R12035 T17744 T17734 arg3Of ),(
R12036 T17745 T17753 arg1Of ",",","
R12037 T17746 T17747 arg1Of 1,%
R12038 T17748 T17745 arg2Of penicillin,","
R12039 T17748 T17746 arg1Of penicillin,1
R12040 T17748 T17749 arg1Of penicillin,(
R12041 T17751 T17749 arg2Of U/ml,(
R12042 T17751 T17750 arg1Of U/ml,10000
R12043 T17752 T17749 arg3Of ),(
R12044 T17753 T17762 arg1Of ",",and
R12045 T17754 T17755 arg1Of 1,%
R12046 T17756 T17753 arg2Of streptomycin,","
R12047 T17756 T17754 arg1Of streptomycin,1
R12048 T17756 T17757 arg1Of streptomycin,(
R12049 T17759 T17757 arg2Of μg/ml,(
R12050 T17759 T17758 arg1Of μg/ml,10000
R12051 T17760 T17757 arg3Of ),(
R12052 T17762 T17703 arg2Of and,or
R12053 T17762 T17761 arg1Of and,","
R12054 T17765 T17766 arg1Of 200,mM
R12055 T17767 T17762 arg2Of L-glutamine,and
R12056 T17767 T17763 arg1Of L-glutamine,1
R12057 T17767 T17764 arg1Of L-glutamine,%
R12058 T17767 T17765 arg1Of L-glutamine,200
R12059 T17767 T17768 arg1Of L-glutamine,(
R12060 T17769 T17768 arg2Of BioWhittaker,(
R12061 T17770 T17768 arg3Of ),(
R12214 T17956 T17955 arg1Of conditions,Stimulation
R12215 T17958 T17957 arg2Of induction,For
R12216 T17958 T17959 arg1Of induction,of
R12217 T17961 T17960 arg1Of factors,transcription
R12218 T17961 T17962 arg1Of factors,and
R12219 T17962 T17959 arg2Of and,of
R12220 T17963 T17962 arg2Of cytokines,and
R12221 T17965 T17966 arg1Of PBMC,were
R12222 T17965 T17967 arg2Of PBMC,cultured
R12223 T17967 T17957 arg1Of cultured,For
R12224 T17967 T17964 arg1Of cultured,","
R12225 T17967 T17966 arg2Of cultured,were
R12226 T17967 T17968 arg1Of cultured,in
R12227 T17967 T17980 arg1Of cultured,(
R12228 T17970 T17968 arg2Of volume,in
R12229 T17970 T17969 arg1Of volume,a
R12230 T17970 T17971 arg1Of volume,of
R12231 T17970 T17974 arg1Of volume,in
R12232 T17973 T17971 arg2Of ml,of
R12233 T17973 T17972 arg1Of ml,4
R12234 T17979 T17974 arg2Of plates,in
R12235 T17979 T17975 arg1Of plates,sterile
R12236 T17979 T17976 arg1Of plates,pyrogen-free
R12237 T17979 T17977 arg1Of plates,6-well
R12238 T17979 T17978 arg1Of plates,culture
R12239 T17981 T17982 arg1Of Falcon,","
R12240 T17982 T17980 arg2Of ",",(
R12241 T17982 T17984 arg1Of ",",","
R12242 T17983 T17982 arg2Of Heidelberg,","
R12243 T17985 T17984 arg2Of Germany,","
R12244 T17986 T17980 arg3Of ),(
R12245 T17987 T17988 arg1Of Cultures,were
R12246 T17987 T17989 arg2Of Cultures,stimulated
R12247 T17989 T17988 arg2Of stimulated,were
R12248 T17989 T17990 arg1Of stimulated,for
R12249 T17989 T17997 arg1Of stimulated,from
R12250 T17989 T18000 arg1Of stimulated,to
R12251 T17989 T18017 arg1Of stimulated,with
R12252 T17989 T18033 arg1Of stimulated,with
R12253 T17989 T18038 arg1Of stimulated,LPS
R12254 T17990 T17996 arg1Of for,or
R12255 T17992 T17990 arg2Of h,for
R12256 T17992 T17991 arg1Of h,1
R12257 T17992 T17993 arg1Of h,(
R12258 T17994 T17993 arg2Of EMSA,(
R12259 T17995 T17993 arg3Of ),(
R12260 T17997 T17996 arg2Of from,or
R12261 T17999 T17997 arg2Of h,from
R12262 T17999 T17998 arg1Of h,0
R12263 T18002 T18000 arg2Of h,to
R12264 T18002 T18001 arg1Of h,48
R12265 T18002 T18003 arg1Of h,(
R12266 T18002 T18006 arg1Of h,with
R12267 T18002 T18016 arg1Of h,","
R12268 T18004 T18003 arg2Of ELISA,(
R12269 T18005 T18003 arg3Of ),(
R12270 T18007 T18008 arg1Of 1,or
R12271 T18009 T18008 arg2Of 100,or
R12272 T18010 T18006 arg2Of μg,with
R12273 T18010 T18007 arg1Of μg,1
R12274 T18010 T18009 arg1Of μg,100
R12275 T18010 T18011 arg1Of μg,per
R12276 T18013 T18011 arg2Of cells,per
R12277 T18013 T18012 arg1Of cells,106
R12278 T18013 T18014 arg1Of cells,of
R12279 T18015 T18014 arg2Of GP,of
R12280 T18017 T18032 arg1Of with,or
R12281 T18020 T18018 arg1Of cells,250
R12282 T18020 T18019 arg1Of cells,ng/106
R12283 T18021 T18017 arg2Of TSST-1,with
R12284 T18021 T18020 arg1Of TSST-1,cells
R12285 T18021 T18022 arg1Of TSST-1,(
R12286 T18024 T18022 arg2Of Technologies,(
R12287 T18024 T18023 arg1Of Technologies,Toxin
R12288 T18024 T18025 arg1Of Technologies,","
R12289 T18024 T18029 arg1Of Technologies,","
R12290 T18026 T18027 arg1Of Sarasota,","
R12291 T18027 T18025 arg2Of ",",","
R12292 T18028 T18027 arg2Of FL,","
R12293 T18030 T18029 arg2Of USA,","
R12294 T18031 T18022 arg3Of ),(
R12295 T18033 T18032 arg2Of with,or
R12296 T18036 T18033 arg2Of cells,with
R12297 T18036 T18034 arg1Of cells,250
R12298 T18036 T18035 arg1Of cells,ng/106
R12299 T18038 T18037 arg1Of LPS,wild-type
R12300 T18038 T18039 arg1Of LPS,from
R12301 T18038 T18044 arg1Of LPS,:
R12302 T18042 T18039 arg2Of serotype,from
R12303 T18042 T18040 arg1Of serotype,Escherichia
R12304 T18042 T18041 arg1Of serotype,coli
R12305 T18042 T18043 arg1Of serotype,0111
R12306 T18045 T18044 arg2Of B4,:
R12307 T18045 T18046 arg1Of B4,(
R12308 T18047 T18048 arg1Of Sigma,","
R12309 T18048 T18046 arg2Of ",",(
R12310 T18048 T18050 arg1Of ",",","
R12311 T18049 T18048 arg2Of Munich,","
R12312 T18051 T18050 arg2Of Germany,","
R12313 T18052 T18046 arg3Of ),(
R12314 T18055 T18053 arg2Of experiments,In
R12315 T18055 T18054 arg1Of experiments,costimulatory
R12316 T18057 T18058 arg1Of PBMC,were
R12317 T18057 T18059 arg2Of PBMC,supplemented
R12318 T18059 T18053 arg1Of supplemented,In
R12319 T18059 T18056 arg1Of supplemented,","
R12320 T18059 T18058 arg2Of supplemented,were
R12321 T18059 T18060 arg1Of supplemented,simultaneously
R12322 T18059 T18061 arg1Of supplemented,with
R12323 T18059 T18069 arg1Of supplemented,with
R12324 T18061 T18068 arg1Of with,or
R12325 T18063 T18061 arg2Of combination,with
R12326 T18063 T18062 arg1Of combination,a
R12327 T18063 T18064 arg1Of combination,of
R12328 T18065 T18066 arg1Of GP,and
R12329 T18066 T18064 arg2Of and,of
R12330 T18067 T18066 arg2Of TSST-1,and
R12331 T18069 T18068 arg2Of with,or
R12332 T18071 T18069 arg2Of combination,with
R12333 T18071 T18070 arg1Of combination,a
R12334 T18071 T18072 arg1Of combination,of
R12335 T18073 T18074 arg1Of GP,and
R12336 T18074 T18072 arg2Of and,of
R12337 T18075 T18074 arg2Of LPS,and
R12470 T18303 T18304 arg1Of Oligonucleotides,(
R12471 T18303 T18307 arg1Of Oligonucleotides,and
R12472 T18305 T18304 arg2Of Oligos,(
R12473 T18306 T18304 arg3Of ),(
R12474 T18308 T18307 arg2Of 5'-32-P-Labeling,and
R12475 T18313 T18312 arg2Of ds,(
R12476 T18314 T18312 arg3Of ),(
R12477 T18315 T18309 arg1Of oligonucleotides,The
R12478 T18315 T18310 arg1Of oligonucleotides,complementary
R12479 T18315 T18311 arg1Of oligonucleotides,double-stranded
R12480 T18315 T18312 arg1Of oligonucleotides,(
R12481 T18315 T18316 arg1Of oligonucleotides,(
R12482 T18315 T18319 arg1Of oligonucleotides,from
R12483 T18315 T18329 arg1Of oligonucleotides,were
R12484 T18315 T18330 arg2Of oligonucleotides,synthesised
R12485 T18315 T18354 arg1Of oligonucleotides,32P-labeled
R12486 T18317 T18316 arg2Of oligos,(
R12487 T18318 T18316 arg3Of ),(
R12488 T18321 T18322 arg1Of TNFα,","
R12489 T18322 T18324 arg1Of ",",","
R12490 T18323 T18322 arg2Of IL-8,","
R12491 T18324 T18326 arg1Of ",",and
R12492 T18325 T18324 arg2Of IFNγ,","
R12493 T18327 T18326 arg2Of IL-6,and
R12494 T18328 T18319 arg2Of promoters,from
R12495 T18328 T18320 arg1Of promoters,the
R12496 T18328 T18321 arg1Of promoters,TNFα
R12497 T18328 T18323 arg1Of promoters,IL-8
R12498 T18328 T18325 arg1Of promoters,IFNγ
R12499 T18328 T18327 arg1Of promoters,IL-6
R12500 T18330 T18331 arg1Of synthesised,from
R12501 T18330 T18353 arg1Of synthesised,and
R12502 T18333 T18332 arg1Of stranded,single
R12503 T18333 T18334 arg1Of stranded,(
R12504 T18335 T18334 arg2Of ss,(
R12505 T18336 T18334 arg3Of ),(
R12506 T18337 T18331 arg2Of oligos,from
R12507 T18337 T18333 arg1Of oligos,stranded
R12508 T18337 T18338 arg1Of oligos,(
R12509 T18337 T18344 arg1Of oligos,","
R12510 T18337 T18345 arg1Of oligos,(
R12511 T18339 T18338 arg2Of illustrated,(
R12512 T18339 T18340 arg1Of illustrated,in
R12513 T18342 T18340 arg2Of 1,in
R12514 T18342 T18341 arg1Of 1,Table
R12515 T18343 T18338 arg3Of ),(
R12516 T18347 T18345 arg2Of Molbiol,(
R12517 T18347 T18346 arg1Of Molbiol,TIB
R12518 T18347 T18348 arg1Of Molbiol,","
R12519 T18349 T18348 arg2Of Berlin,","
R12520 T18349 T18350 arg1Of Berlin,","
R12521 T18351 T18350 arg2Of Germany,","
R12522 T18352 T18345 arg3Of ),(
R12523 T18353 T18329 arg2Of and,were
R12524 T18354 T18353 arg2Of 32P-labeled,and
R12525 T18354 T18355 arg1Of 32P-labeled,with
R12526 T18356 T18355 arg2Of 5'γ-P-ATP,with
R12527 T18356 T18357 arg1Of 5'γ-P-ATP,(
R12528 T18356 T18367 arg1Of 5'γ-P-ATP,using
R12529 T18359 T18357 arg2Of Ci/mmol,(
R12530 T18359 T18358 arg1Of Ci/mmol,"3,000"
R12531 T18359 T18360 arg1Of Ci/mmol,","
R12532 T18359 T18364 arg1Of Ci/mmol,","
R12533 T18361 T18362 arg1Of Amersham,","
R12534 T18362 T18360 arg2Of ",",","
R12535 T18363 T18362 arg2Of Braunschweig,","
R12536 T18365 T18364 arg2Of Germany,","
R12537 T18366 T18357 arg3Of ),(
R12538 T18367 T18382 arg1Of using,according
R12539 T18370 T18367 arg2Of Kit,using
R12540 T18370 T18368 arg1Of Kit,the
R12541 T18370 T18369 arg1Of Kit,Ready-To-Go-Polynucleotide-Kinase
R12542 T18370 T18371 arg1Of Kit,(
R12543 T18373 T18372 arg1Of Kit,PNK
R12544 T18373 T18374 arg1Of Kit,","
R12545 T18373 T18377 arg1Of Kit,","
R12546 T18376 T18374 arg2Of LKB,","
R12547 T18376 T18375 arg1Of LKB,Pharmacia
R12548 T18377 T18371 arg2Of ",",(
R12549 T18378 T18377 arg2Of Freiburg,","
R12550 T18378 T18379 arg1Of Freiburg,","
R12551 T18380 T18379 arg2Of Germany,","
R12552 T18381 T18371 arg3Of ),(
R12553 T18383 T18382 arg2Of to,according
R12554 T18385 T18384 arg1Of manufacturer,the
R12555 T18385 T18386 arg2Of manufacturer,'s
R12556 T18387 T18383 arg2Of instructions,to
R12557 T18387 T18386 arg1Of instructions,'s
R12558 T18389 T18388 arg1Of test,To
R12559 T18392 T18389 arg2Of specificity,test
R12560 T18392 T18390 arg1Of specificity,the
R12561 T18392 T18391 arg1Of specificity,binding
R12562 T18392 T18393 arg1Of specificity,of
R12563 T18395 T18393 arg2Of oligos,of
R12564 T18395 T18394 arg1Of oligos,the
R12565 T18398 T18397 arg2Of oligos,mutated
R12566 T18398 T18399 arg1Of oligos,were
R12567 T18398 T18400 arg2Of oligos,used
R12568 T18400 T18388 modOf used,To
R12569 T18400 T18396 arg1Of used,","
R12570 T18400 T18399 arg2Of used,were
R12571 T18400 T18401 arg1Of used,as
R12572 T18404 T18401 arg2Of control,as
R12573 T18404 T18402 arg1Of control,an
R12574 T18404 T18403 arg1Of control,additional
R12575 T18405 T18406 arg1Of Mutations,in
R12576 T18405 T18418 arg1Of Mutations,in
R12577 T18405 T18427 arg1Of Mutations,[
R12578 T18405 T18430 arg1Of Mutations,have
R12579 T18405 T18431 arg1Of Mutations,been
R12580 T18405 T18432 arg2Of Mutations,reported
R12581 T18405 T18434 arg1Of Mutations,interfere
R12582 T18406 T18417 arg1Of in,and
R12583 T18409 T18406 arg2Of site,in
R12584 T18409 T18407 arg1Of site,the
R12585 T18409 T18408 arg1Of site,P2
R12586 T18409 T18410 arg1Of site,of
R12587 T18413 T18410 arg2Of promoter,of
R12588 T18413 T18411 arg1Of promoter,the
R12589 T18413 T18412 arg1Of promoter,IFNγ
R12590 T18413 T18414 arg1Of promoter,[
R12591 T18415 T18414 arg2Of 29,[
R12592 T18416 T18414 arg3Of ],[
R12593 T18418 T18417 arg2Of in,and
R12594 T18422 T18418 arg2Of sequence,in
R12595 T18422 T18419 arg1Of sequence,the
R12596 T18422 T18420 arg1Of sequence,κ
R12597 T18422 T18421 arg1Of sequence,consensus
R12598 T18422 T18423 arg1Of sequence,of
R12599 T18426 T18423 arg2Of promoter,of
R12600 T18426 T18424 arg1Of promoter,the
R12601 T18426 T18425 arg1Of promoter,TNFα
R12602 T18428 T18427 arg2Of 55,[
R12603 T18429 T18427 arg3Of ],[
R12604 T18432 T18430 arg2Of reported,have
R12605 T18432 T18431 arg2Of reported,been
R12606 T18434 T18432 arg3Of interfere,reported
R12607 T18434 T18433 arg1Of interfere,to
R12608 T18434 T18435 arg1Of interfere,with
R12609 T18436 T18435 arg2Of sequences,with
R12610 T18436 T18437 arg1Of sequences,","
R12611 T18436 T18438 arg1Of sequences,which
R12612 T18436 T18439 arg1Of sequences,seem
R12613 T18436 T18441 arg1Of sequences,be
R12614 T18436 T18442 arg1Of sequences,crucial
R12615 T18441 T18439 arg2Of be,seem
R12616 T18441 T18440 arg1Of be,to
R12617 T18441 T18451 arg1Of be,","
R12618 T18441 T18452 arg1Of be,respectively
R12619 T18442 T18441 arg2Of crucial,be
R12620 T18442 T18443 arg1Of crucial,for
R12621 T18445 T18443 arg2Of binding,for
R12622 T18445 T18444 arg1Of binding,the
R12623 T18445 T18446 arg1Of binding,of
R12624 T18447 T18448 arg1Of NFAT,and
R12625 T18449 T18448 arg2Of NFκB,and
R12626 T18450 T18446 arg2Of proteins,of
R12627 T18450 T18447 arg1Of proteins,NFAT
R12628 T18450 T18449 arg1Of proteins,NFκB
R12629 T18457 T18455 arg1Of oligos,the
R12630 T18457 T18456 arg1Of oligos,ds
R12631 T18457 T18458 arg1Of oligos,were
R12632 T18457 T18459 arg2Of oligos,purified
R12633 T18457 T18463 arg1Of oligos,using
R12634 T18459 T18453 arg1Of purified,Afterwards
R12635 T18459 T18454 arg1Of purified,","
R12636 T18459 T18458 arg2Of purified,were
R12637 T18459 T18460 arg1Of purified,via
R12638 T18459 T18463 modOf purified,using
R12639 T18462 T18460 arg2Of filtration,via
R12640 T18462 T18461 arg1Of filtration,gel
R12641 T18468 T18464 arg1Of Columns,Probe
R12642 T18468 T18465 arg1Of Columns,Quant
R12643 T18468 T18466 arg1Of Columns,G-50
R12644 T18468 T18467 arg1Of Columns,Micro
R12645 T18468 T18469 arg1Of Columns,(
R12646 T18468 T18472 arg1Of Columns,according
R12647 T18470 T18469 arg2Of Pharmacia,(
R12648 T18471 T18469 arg3Of ),(
R12649 T18473 T18472 arg2Of to,according
R12650 T18475 T18474 arg1Of manufacturer,the
R12651 T18475 T18476 arg2Of manufacturer,'s
R12652 T18476 T18473 arg2Of 's,to
R12653 T18477 T18463 arg2Of instructions,using
R12654 T18477 T18468 arg1Of instructions,Columns
R12866 T18814 T18815 arg1Of Preparation,of
R12867 T18817 T18816 arg1Of extracts,nuclear
R12868 T18817 T18818 arg1Of extracts,and
R12869 T18818 T18815 arg2Of and,of
R12870 T18822 T18818 arg2Of assays,and
R12871 T18822 T18819 arg1Of assays,electrophoretic
R12872 T18822 T18820 arg1Of assays,mobility
R12873 T18822 T18821 arg1Of assays,shift
R12874 T18822 T18823 arg1Of assays,(
R12875 T18824 T18823 arg2Of EMSA,(
R12876 T18825 T18823 arg3Of ),(
R12877 T18827 T18826 arg1Of extracts,Nuclear
R12878 T18827 T18828 arg1Of extracts,of
R12879 T18827 T18831 arg1Of extracts,were
R12880 T18827 T18832 arg2Of extracts,prepared
R12881 T18830 T18828 arg2Of PBMCs,of
R12882 T18830 T18829 arg2Of PBMCs,purified
R12883 T18832 T18831 arg2Of prepared,were
R12884 T18832 T18833 arg1Of prepared,according
R12885 T18834 T18833 arg2Of to,according
R12886 T18836 T18834 arg2Of technique,to
R12887 T18836 T18835 arg1Of technique,a
R12888 T18836 T18837 arg2Of technique,described
R12889 T18836 T18846 arg2Of technique,modified
R12890 T18837 T18842 arg1Of described,[
R12891 T18837 T18845 arg1Of described,and
R12892 T18839 T18837 arg1Of Trede,described
R12893 T18839 T18838 arg2Of Trede,by
R12894 T18839 T18841 arg1Of Trede,al.
R12895 T18841 T18840 arg1Of al.,et
R12896 T18843 T18842 arg2Of 27,[
R12897 T18844 T18842 arg3Of ],[
R12898 T18846 T18845 arg2Of modified,and
R12899 T18846 T18847 arg1Of modified,for
R12900 T18850 T18847 arg2Of setup,for
R12901 T18850 T18848 arg1Of setup,our
R12902 T18850 T18849 arg1Of setup,experimental
R12903 T18850 T18851 arg1Of setup,[
R12904 T18852 T18851 arg2Of 56,[
R12905 T18853 T18851 arg3Of ],[
R12906 T18855 T18854 arg1Of intensity,The
R12907 T18855 T18856 arg1Of intensity,of
R12908 T18855 T18859 arg1Of intensity,was
R12909 T18855 T18860 arg2Of intensity,normalized
R12910 T18858 T18856 arg2Of bands,of
R12911 T18858 T18857 arg2Of bands,shifted
R12912 T18860 T18859 arg2Of normalized,was
R12913 T18860 T18861 arg1Of normalized,to
R12914 T18862 T18861 arg2Of bands,to
R12915 T18862 T18863 arg1Of bands,of
R12916 T18865 T18863 arg2Of controls,of
R12917 T18865 T18864 arg1Of controls,unstimulated
R12918 T18867 T18866 arg1Of experiments,Competition
R12919 T18867 T18868 arg1Of experiments,with
R12920 T18867 T18869 arg2Of experiments,labeled
R12921 T18867 T18884 arg1Of experiments,were
R12922 T18867 T18885 arg2Of experiments,carried
R12923 T18867 T18890 arg1Of experiments,prevent
R12924 T18869 T18880 arg1Of labeled,(
R12925 T18873 T18869 arg3Of oligos,labeled
R12926 T18873 T18870 arg1Of oligos,and
R12927 T18873 T18871 arg1Of oligos,unlabeled
R12928 T18873 T18872 arg2Of oligos,mutated
R12929 T18873 T18874 arg1Of oligos,of
R12930 T18879 T18874 arg2Of sites,of
R12931 T18879 T18875 arg1Of sites,the
R12932 T18879 T18876 arg1Of sites,cytokine
R12933 T18879 T18877 arg1Of sites,promoter
R12934 T18879 T18878 arg1Of sites,binding
R12935 T18881 T18880 arg2Of see,(
R12936 T18881 T18882 arg1Of see,above
R12937 T18883 T18880 arg3Of ),(
R12938 T18885 T18884 arg2Of carried,were
R12939 T18885 T18886 arg1Of carried,out
R12940 T18885 T18887 arg1Of carried,in
R12941 T18888 T18887 arg2Of order,in
R12942 T18890 T18887 arg3Of prevent,in
R12943 T18890 T18889 arg1Of prevent,to
R12944 T18892 T18890 arg2Of binding,prevent
R12945 T18892 T18891 arg1Of binding,non-specific
R12946 T18892 T18893 arg1Of binding,to
R12947 T18895 T18893 arg2Of proteins,to
R12948 T18895 T18894 arg1Of proteins,nuclear
R12949 T18898 T18896 arg2Of experiments,For
R12950 T18898 T18897 arg1Of experiments,such
R12951 T18903 T18900 arg1Of 5–50×,an
R12952 T18903 T18901 arg1Of 5–50×,excess
R12953 T18903 T18902 arg2Of 5–50×,(
R12954 T18903 T18905 arg1Of 5–50×,of
R12955 T18903 T18908 arg1Of 5–50×,was
R12956 T18903 T18909 arg2Of 5–50×,added
R12957 T18904 T18902 arg3Of ),(
R12958 T18907 T18905 arg2Of oligos,of
R12959 T18907 T18906 arg1Of oligos,unlabeled
R12960 T18909 T18896 arg1Of added,For
R12961 T18909 T18899 arg1Of added,","
R12962 T18909 T18908 arg2Of added,was
R12963 T18909 T18910 arg1Of added,to
R12964 T18913 T18910 arg2Of extracts,to
R12965 T18913 T18911 arg1Of extracts,the
R12966 T18913 T18912 arg1Of extracts,nuclear
R12967 T18915 T18914 arg2Of specificity,For
R12968 T18915 T18916 arg1Of specificity,of
R12969 T18917 T18916 arg2Of binding,of
R12970 T18920 T18919 arg1Of assays,supershift
R12971 T18920 T18921 arg1Of assays,for
R12972 T18920 T18928 arg1Of assays,were
R12973 T18920 T18929 arg2Of assays,performed
R12974 T18922 T18921 arg2Of NFκB,for
R12975 T18922 T18923 arg1Of NFκB,with
R12976 T18927 T18923 arg2Of antibody,with
R12977 T18927 T18924 arg1Of antibody,a
R12978 T18927 T18925 arg1Of antibody,combined
R12979 T18927 T18926 arg1Of antibody,p65/50
R12980 T18929 T18914 arg1Of performed,For
R12981 T18929 T18918 arg1Of performed,","
R12982 T18929 T18928 arg2Of performed,were
R12983 T18929 T18930 arg1Of performed,(
R12984 T18931 T18930 arg2Of data,(
R12985 T18931 T18933 arg2Of data,shown
R12986 T18933 T18932 arg1Of shown,not
R12987 T18934 T18930 arg3Of ),(
R12988 T18936 T18935 arg1Of exclude,To
R12989 T18938 T18936 arg2Of reactions,exclude
R12990 T18938 T18937 arg1Of reactions,non-specific
R12991 T18943 T18936 arg1Of excess,exclude
R12992 T18943 T18940 arg1Of excess,a
R12993 T18943 T18941 arg1Of excess,30-fold
R12994 T18943 T18942 arg1Of excess,molar
R12995 T18943 T18944 arg1Of excess,of
R12996 T18943 T18947 arg1Of excess,(
R12997 T18943 T18954 arg1Of excess,was
R12998 T18943 T18955 arg2Of excess,used
R12999 T18946 T18944 arg2Of DNA,of
R13000 T18946 T18945 arg1Of DNA,Oct-1
R13001 T18949 T18947 arg2Of DNA,(
R13002 T18949 T18948 arg1Of DNA,unrelated
R13003 T18949 T18950 arg1Of DNA,","
R13004 T18949 T18951 arg1Of DNA,Table
R13005 T18951 T18952 arg1Of Table,1
R13006 T18953 T18947 arg3Of ),(
R13007 T18955 T18935 modOf used,To
R13008 T18955 T18939 arg1Of used,","
R13009 T18955 T18954 arg2Of used,was
R13010 T18955 T18956 arg1Of used,","
R13011 T18955 T18957 arg1Of used,which
R13012 T18955 T18958 arg1Of used,did
R13013 T18955 T18960 arg1Of used,compete
R13014 T18960 T18958 arg2Of compete,did
R13015 T18960 T18959 arg1Of compete,not
R13016 T18960 T18961 arg1Of compete,with
R13017 T18963 T18961 arg2Of binding,with
R13018 T18963 T18962 arg1Of binding,specific
R13198 T19288 T19285 arg2Of membrane,onto
R13199 T19288 T19286 arg1Of membrane,a
R13200 T19288 T19287 arg1Of membrane,nitrocellulose
R13201 T19288 T19289 arg1Of membrane,(
R13202 T19290 T19289 arg2Of Bio-Rad,(
R13203 T19291 T19289 arg3Of ),(
R13204 T19293 T19292 arg2Of blocking,After
R13205 T19293 T19294 arg1Of blocking,with
R13206 T19293 T19298 arg1Of blocking,(
R13207 T19297 T19294 arg2Of BSA,with
R13208 T19297 T19295 arg1Of BSA,PBS/3
R13209 T19297 T19296 arg1Of BSA,%
R13210 T19299 T19300 arg1Of Fluka,","
R13211 T19300 T19302 arg1Of ",",","
R13212 T19301 T19300 arg2Of Deisenhofen,","
R13213 T19302 T19298 arg2Of ",",(
R13214 T19303 T19302 arg2Of Germany,","
R13215 T19304 T19298 arg3Of ),(
R13216 T19307 T19293 arg1Of blot,blocking
R13217 T19307 T19306 arg1Of blot,the
R13218 T19307 T19308 arg1Of blot,was
R13219 T19307 T19309 arg2Of blot,incubated
R13220 T19309 T19292 arg1Of incubated,After
R13221 T19309 T19305 arg1Of incubated,","
R13222 T19309 T19308 arg2Of incubated,was
R13223 T19309 T19310 arg1Of incubated,overnight
R13224 T19309 T19311 arg1Of incubated,with
R13225 T19309 T19317 arg1Of incubated,:
R13226 T19314 T19311 arg2Of antibodies,with
R13227 T19314 T19312 arg1Of antibodies,the
R13228 T19314 T19313 arg1Of antibodies,primary
R13229 T19314 T19315 arg2Of antibodies,diluted
R13230 T19316 T19315 arg3Of 1,diluted
R13231 T19318 T19319 arg1Of 2000,in
R13232 T19318 T19324 arg1Of 2000,washed
R13233 T19318 T19327 arg2Of 2000,incubated
R13234 T19322 T19319 arg2Of BSA,in
R13235 T19322 T19320 arg1Of BSA,PBS/1
R13236 T19322 T19321 arg1Of BSA,%
R13237 T19324 T19325 arg1Of washed,again
R13238 T19324 T19326 arg1Of washed,and
R13239 T19326 T19317 arg2Of and,:
R13240 T19326 T19323 arg1Of and,","
R13241 T19327 T19326 arg2Of incubated,and
R13242 T19327 T19328 arg1Of incubated,with
R13243 T19333 T19328 arg2Of antibody,with
R13244 T19333 T19329 arg1Of antibody,the
R13245 T19333 T19330 arg1Of antibody,alkaline
R13246 T19333 T19331 arg1Of antibody,phosphatase-conjugated
R13247 T19333 T19332 arg1Of antibody,secondary
R13248 T19333 T19334 arg1Of antibody,(
R13249 T19335 T19336 arg1Of 1,:
R13250 T19336 T19334 arg2Of :,(
R13251 T19336 T19338 arg1Of :,in
R13252 T19337 T19336 arg2Of 500,:
R13253 T19341 T19338 arg2Of BSA,in
R13254 T19341 T19339 arg1Of BSA,PBS/1
R13255 T19341 T19340 arg1Of BSA,%
R13256 T19342 T19334 arg3Of ),(
R13257 T19344 T19343 arg1Of blot,The
R13258 T19344 T19345 arg1Of blot,was
R13259 T19344 T19346 arg2Of blot,developed
R13260 T19346 T19345 arg2Of developed,was
R13261 T19347 T19346 arg3Of using,developed
R13262 T19351 T19347 arg2Of kit,using
R13263 T19351 T19348 arg1Of kit,the
R13264 T19351 T19349 arg1Of kit,Vectastain®
R13265 T19351 T19350 arg1Of kit,staining
R13266 T19351 T19352 arg1Of kit,(
R13267 T19356 T19352 arg2Of Burlingame,(
R13268 T19356 T19353 arg1Of Burlingame,Vector
R13269 T19356 T19354 arg1Of Burlingame,Laboratories
R13270 T19356 T19355 arg1Of Burlingame,Inc.
R13271 T19356 T19357 arg1Of Burlingame,","
R13272 T19358 T19357 arg2Of CA,","
R13273 T19358 T19359 arg1Of CA,","
R13274 T19360 T19359 arg2Of USA,","
R13275 T19361 T19352 arg3Of ),(
R13522 T19802 T19801 arg1Of measurements,Cytokine
R13523 T19803 T19804 arg1Of Supernatants,of
R13524 T19803 T19807 arg1Of Supernatants,were
R13525 T19803 T19808 arg2Of Supernatants,harvested
R13526 T19803 T19830 arg1Of Supernatants,stored
R13527 T19806 T19804 arg2Of cultures,of
R13528 T19806 T19805 arg1Of cultures,cell
R13529 T19808 T19807 arg2Of harvested,were
R13530 T19808 T19829 arg1Of harvested,and
R13531 T19809 T19810 arg1Of 3,","
R13532 T19810 T19812 arg1Of ",",","
R13533 T19811 T19810 arg2Of 6,","
R13534 T19812 T19814 arg1Of ",",","
R13535 T19813 T19812 arg2Of 9,","
R13536 T19814 T19816 arg1Of ",",","
R13537 T19815 T19814 arg2Of 12,","
R13538 T19816 T19818 arg1Of ",",","
R13539 T19817 T19816 arg2Of 18,","
R13540 T19818 T19820 arg1Of ",",","
R13541 T19819 T19818 arg2Of 24,","
R13542 T19821 T19820 arg2Of 30,","
R13543 T19823 T19824 arg1Of 36,and
R13544 T19825 T19824 arg2Of 48,and
R13545 T19826 T19808 arg3Of hours,harvested
R13546 T19826 T19809 arg1Of hours,3
R13547 T19826 T19811 arg1Of hours,6
R13548 T19826 T19813 arg1Of hours,9
R13549 T19826 T19815 arg1Of hours,12
R13550 T19826 T19817 arg1Of hours,18
R13551 T19826 T19819 arg1Of hours,24
R13552 T19826 T19821 arg1Of hours,30
R13553 T19826 T19822 arg1Of hours,","
R13554 T19826 T19823 arg1Of hours,36
R13555 T19826 T19825 arg1Of hours,48
R13556 T19826 T19827 arg1Of hours,after
R13557 T19828 T19827 arg2Of stimulation,after
R13558 T19830 T19829 arg2Of stored,and
R13559 T19830 T19831 arg1Of stored,at
R13560 T19830 T19834 arg1Of stored,until
R13561 T19833 T19831 arg2Of °C,at
R13562 T19833 T19832 arg1Of °C,-80
R13563 T19835 T19836 arg1Of used,and
R13564 T19836 T19834 arg2Of and,until
R13565 T19837 T19836 arg2Of thawed,and
R13566 T19837 T19839 arg1Of thawed,once
R13567 T19839 T19838 arg1Of once,only
R13568 T19840 T19841 arg1Of IFNγ,","
R13569 T19841 T19843 arg1Of ",",","
R13570 T19842 T19841 arg2Of TNFα,","
R13571 T19843 T19845 arg1Of ",",and
R13572 T19844 T19843 arg2Of IL-1,","
R13573 T19845 T19847 arg1Of and,were
R13574 T19845 T19848 arg2Of and,determined
R13575 T19846 T19845 arg2Of IL-6,and
R13576 T19848 T19847 arg2Of determined,were
R13577 T19848 T19849 modOf determined,using
R13578 T19848 T19864 arg1Of determined,by
R13579 T19851 T19849 arg2Of ELISA,using
R13580 T19851 T19850 arg1Of ELISA,an
R13581 T19851 T19852 arg1Of ELISA,from
R13582 T19854 T19853 arg1Of Systems,Bender
R13583 T19854 T19855 arg1Of Systems,(
R13584 T19854 T19860 arg1Of Systems,","
R13585 T19856 T19855 arg2Of Vienna,(
R13586 T19856 T19857 arg1Of Vienna,","
R13587 T19856 T19858 arg1Of Vienna,Austria
R13588 T19859 T19855 arg3Of ),(
R13589 T19860 T19862 arg1Of ",",and
R13590 T19861 T19860 arg2Of IL-1RA,","
R13591 T19862 T19852 arg2Of and,from
R13592 T19863 T19862 arg2Of IL-8,and
R13593 T19866 T19867 arg1Of ELISA,from
R13594 T19866 T19878 arg1Of ELISA,and
R13595 T19871 T19867 arg2Of Systems,from
R13596 T19871 T19868 arg1Of Systems,R
R13597 T19871 T19869 arg1Of Systems,&
R13598 T19871 T19870 arg1Of Systems,D
R13599 T19871 T19872 arg1Of Systems,(
R13600 T19873 T19872 arg2Of Wiesbaden-Nordenstadt,(
R13601 T19873 T19874 arg1Of Wiesbaden-Nordenstadt,","
R13602 T19875 T19874 arg2Of Germany,","
R13603 T19876 T19872 arg3Of ),(
R13604 T19878 T19864 arg2Of and,by
R13605 T19878 T19865 arg1Of and,an
R13606 T19878 T19877 arg1Of and,","
R13607 T19879 T19878 arg2Of TGFβ,and
R13608 T19879 T19880 arg1Of TGFβ,1
R13609 T19881 T19848 arg3Of using,determined
R13610 T19881 T19884 arg1Of using,as
R13611 T19883 T19881 arg2Of ELISA,using
R13612 T19883 T19882 arg1Of ELISA,an
R13613 T19886 T19885 arg1Of described,previously
R13614 T19888 T19884 arg2Of 57,as
R13615 T19888 T19886 arg1Of 57,described
R13616 T19888 T19887 arg2Of 57,[
R13617 T19889 T19887 arg3Of ],[
R13618 T19890 T19891 arg1Of Quantification,of
R13619 T19890 T19893 arg1Of Quantification,as
R13620 T19890 T19901 arg1Of Quantification,was
R13621 T19890 T19902 arg2Of Quantification,done
R13622 T19892 T19891 arg2Of IL-2,of
R13623 T19895 T19893 arg2Of indicator,as
R13624 T19895 T19894 arg1Of indicator,an
R13625 T19895 T19896 arg1Of indicator,for
R13626 T19898 T19896 arg2Of bioactivity,for
R13627 T19898 T19897 arg1Of bioactivity,the
R13628 T19898 T19899 arg1Of bioactivity,of
R13629 T19900 T19899 arg2Of IL-1,of
R13630 T19902 T19901 arg2Of done,was
R13631 T19902 T19903 arg1Of done,as
R13632 T19905 T19904 arg1Of described,previously
R13633 T19907 T19903 arg2Of 46,as
R13634 T19907 T19905 arg1Of 46,described
R13635 T19907 T19906 arg2Of 46,[
R13636 T19908 T19906 arg3Of ],[
R13637 T19909 T19910 arg1Of Samples,were
R13638 T19909 T19911 arg2Of Samples,diluted
R13639 T19911 T19910 arg2Of diluted,were
R13640 T19911 T19912 arg1Of diluted,in
R13641 T19911 T19916 arg1Of diluted,as
R13642 T19915 T19912 arg2Of buffer,in
R13643 T19915 T19913 arg1Of buffer,the
R13644 T19915 T19914 arg1Of buffer,same
R13645 T19917 T19916 arg2Of that,as
R13646 T19917 T19918 arg2Of that,used
R13647 T19918 T19919 arg1Of used,for
R13648 T19921 T19919 arg2Of standards,for
R13649 T19921 T19920 arg1Of standards,the
R13650 T19923 T19922 arg1Of cytokines,All
R13651 T19923 T19924 arg1Of cytokines,were
R13652 T19923 T19925 arg2Of cytokines,quantified
R13653 T19925 T19924 arg2Of quantified,were
R13654 T19926 T19925 arg3Of using,quantified
R13655 T19926 T19931 arg1Of using,(
R13656 T19930 T19926 arg2Of reader,using
R13657 T19930 T19927 arg1Of reader,an
R13658 T19930 T19928 arg1Of reader,ELISA
R13659 T19930 T19929 arg1Of reader,plate
R13660 T19933 T19934 arg1Of Labotec,","
R13661 T19934 T19936 arg1Of ",",","
R13662 T19935 T19934 arg2Of Salzburg,","
R13663 T19936 T19938 arg1Of ",",or
R13664 T19937 T19936 arg2Of Austria,","
R13665 T19939 T19938 arg2Of Microplate,or
R13666 T19940 T19931 arg2Of Reader,(
R13667 T19940 T19932 arg1Of Reader,Anthos
R13668 T19940 T19933 arg1Of Reader,Labotec
R13669 T19940 T19935 arg1Of Reader,Salzburg
R13670 T19940 T19937 arg1Of Reader,Austria
R13671 T19940 T19939 arg1Of Reader,Microplate
R13672 T19940 T19941 arg1Of Reader,","
R13673 T19942 T19941 arg2Of BioRad,","
R13674 T19943 T19931 arg3Of ),(
R13675 T19945 T19944 arg1Of amounts,Cytokine
R13676 T19945 T19946 arg1Of amounts,were
R13677 T19945 T19948 arg1Of amounts,within
R13678 T19946 T19947 arg1Of were,all
R13679 T19948 T19946 arg2Of within,were
R13680 T19950 T19948 arg2Of range,within
R13681 T19950 T19949 arg1Of range,the
R13682 T19950 T19951 arg1Of range,of
R13683 T19954 T19951 arg2Of curve,of
R13684 T19954 T19952 arg1Of curve,the
R13685 T19954 T19953 arg1Of curve,standard
R13686 T19956 T19955 arg1Of stimulation,Only
R13687 T19956 T19957 arg1Of stimulation,of
R13688 T19956 T19967 arg1Of stimulation,was
R13689 T19956 T19968 arg2Of stimulation,included
R13690 T19958 T19957 arg2Of PBMC,of
R13691 T19958 T19959 arg2Of PBMC,observed
R13692 T19959 T19960 arg1Of observed,in
R13693 T19961 T19960 arg2Of donors,in
R13694 T19961 T19962 arg1Of donors,showing
R13695 T19966 T19962 arg2Of release,showing
R13696 T19966 T19963 arg1Of release,no
R13697 T19966 T19964 arg1Of release,spontaneous
R13698 T19966 T19965 arg1Of release,cytokine
R13699 T19968 T19967 arg2Of included,was
R13700 T19968 T19969 arg1Of included,in
R13701 T19971 T19969 arg2Of statistics,in
R13702 T19971 T19970 arg1Of statistics,the
R13892 T20332 T20333 arg1Of Inhibition,of
R13893 T20335 T20333 arg2Of factors,of
R13894 T20335 T20334 arg1Of factors,transcription
R13895 T20337 T20336 arg1Of inhibit,To
R13896 T20338 T20339 arg1Of translocation,and
R13897 T20339 T20337 arg2Of and,inhibit
R13898 T20340 T20339 arg2Of binding,and
R13899 T20340 T20341 arg1Of binding,of
R13900 T20340 T20344 arg1Of binding,to
R13901 T20343 T20341 arg2Of factors,of
R13902 T20343 T20342 arg1Of factors,transcription
R13903 T20347 T20344 arg2Of promoter,to
R13904 T20347 T20345 arg1Of promoter,the
R13905 T20347 T20346 arg1Of promoter,IL-1RA
R13906 T20349 T20337 arg1Of PBMC,inhibit
R13907 T20349 T20350 arg1Of PBMC,were
R13908 T20349 T20351 arg2Of PBMC,incubated
R13909 T20351 T20336 modOf incubated,To
R13910 T20351 T20348 arg1Of incubated,","
R13911 T20351 T20350 arg2Of incubated,were
R13912 T20351 T20352 arg1Of incubated,for
R13913 T20351 T20355 arg1Of incubated,with
R13914 T20351 T20361 arg1Of incubated,(
R13915 T20351 T20370 arg1Of incubated,:
R13916 T20354 T20352 arg2Of h,for
R13917 T20354 T20353 arg1Of h,1
R13918 T20356 T20355 arg2Of each,with
R13919 T20356 T20357 arg1Of each,of
R13920 T20360 T20357 arg2Of substances,of
R13921 T20360 T20358 arg1Of substances,the
R13922 T20360 T20359 arg1Of substances,following
R13923 T20362 T20363 arg1Of concentrations,were
R13924 T20362 T20364 arg2Of concentrations,based
R13925 T20364 T20361 arg2Of based,(
R13926 T20364 T20363 arg2Of based,were
R13927 T20364 T20365 arg1Of based,upon
R13928 T20366 T20367 arg1Of 50,%
R13929 T20368 T20365 arg2Of inhibition,upon
R13930 T20368 T20366 arg1Of inhibition,50
R13931 T20369 T20361 arg3Of ),(
R13932 T20372 T20371 arg1Of μg/ml,50
R13933 T20372 T20373 arg1Of μg/ml,of
R13934 T20372 T20386 arg1Of μg/ml,;
R13935 T20374 T20373 arg2Of CAPE,of
R13936 T20374 T20375 arg1Of CAPE,(
R13937 T20374 T20378 arg1Of CAPE,","
R13938 T20374 T20379 arg1Of CAPE,(
R13939 T20376 T20375 arg2Of "caffeic-3,4-dihydroxycinnamic-acid-phenyl-ester",(
R13940 T20377 T20375 arg3Of ),(
R13941 T20380 T20381 arg1Of Biomol,","
R13942 T20381 T20379 arg2Of ",",(
R13943 T20381 T20383 arg1Of ",",","
R13944 T20382 T20381 arg2Of Hamburg,","
R13945 T20384 T20383 arg2Of Germany,","
R13946 T20385 T20379 arg3Of ),(
R13947 T20387 T20388 arg1Of 400,ng/ml
R13948 T20390 T20387 arg1Of A,400
R13949 T20390 T20389 arg1Of A,cyclosporin
R13950 T20390 T20391 arg1Of A,(
R13951 T20390 T20400 arg1Of A,;
R13952 T20393 T20391 arg2Of Biosciences,(
R13953 T20393 T20392 arg1Of Biosciences,Calbiochem-Merck
R13954 T20393 T20394 arg1Of Biosciences,","
R13955 T20396 T20394 arg2Of Soden,","
R13956 T20396 T20395 arg1Of Soden,Bad
R13957 T20396 T20397 arg1Of Soden,","
R13958 T20398 T20397 arg2Of Germany,","
R13959 T20399 T20391 arg3Of ),(
R13960 T20400 T20386 arg2Of ;,;
R13961 T20400 T20409 arg1Of ;,prior
R13962 T20403 T20400 arg2Of cycloheximide,;
R13963 T20403 T20401 arg1Of cycloheximide,10
R13964 T20403 T20402 arg1Of cycloheximide,μg/ml
R13965 T20403 T20404 arg1Of cycloheximide,(
R13966 T20407 T20404 arg2Of Biotechnology,(
R13967 T20407 T20405 arg1Of Biotechnology,Santa
R13968 T20407 T20406 arg1Of Biotechnology,Cruz
R13969 T20408 T20404 arg3Of ),(
R13970 T20409 T20410 arg1Of prior,to
R13971 T20411 T20410 arg2Of incubation,to
R13972 T20411 T20412 arg1Of incubation,with
R13973 T20413 T20412 arg2Of GP,with
R13974 T20415 T20414 arg1Of shifts,Band
R13975 T20415 T20416 arg1Of shifts,were
R13976 T20415 T20417 arg2Of shifts,determined
R13977 T20417 T20416 arg2Of determined,were
R13978 T20417 T20418 arg1Of determined,after
R13979 T20419 T20420 arg1Of 1,h
R13980 T20421 T20419 arg1Of incubation,1
R13981 T20421 T20422 arg1Of incubation,with
R13982 T20421 T20424 arg1Of incubation,as
R13983 T20421 T20427 arg1Of incubation,","
R13984 T20421 T20428 arg1Of incubation,including
R13985 T20421 T20445 arg1Of incubation,and
R13986 T20423 T20422 arg2Of GP,with
R13987 T20425 T20424 arg2Of described,as
R13988 T20425 T20426 arg1Of described,before
R13989 T20430 T20428 arg2Of oligos,including
R13990 T20430 T20429 arg2Of oligos,mutated
R13991 T20430 T20431 arg1Of oligos,for
R13992 T20432 T20431 arg2Of NFIL-6,for
R13993 T20432 T20433 arg1Of NFIL-6,(
R13994 T20442 T20434 arg1Of AGT,TTA
R13995 T20442 T20435 arg1Of AGT,CAA
R13996 T20442 T20436 arg1Of AGT,CAG
R13997 T20442 T20437 arg1Of AGT,TGG
R13998 T20442 T20438 arg1Of AGT,ATT
R13999 T20442 T20439 arg1Of AGT,GCG
R14000 T20442 T20440 arg1Of AGT,ACA
R14001 T20442 T20441 arg1Of AGT,CTT
R14002 T20443 T20433 arg2Of GGG,(
R14003 T20443 T20442 arg1Of GGG,AGT
R14004 T20444 T20433 arg3Of ),(
R14005 T20445 T20418 arg2Of and,after
R14006 T20446 T20447 arg1Of NFATP2/3,(
R14007 T20457 T20447 arg2Of TCT,(
R14008 T20457 T20448 arg1Of TCT,GGC
R14009 T20457 T20449 arg1Of TCT,GCA
R14010 T20457 T20450 arg1Of TCT,GAA
R14011 T20457 T20451 arg1Of TCT,AAG
R14012 T20457 T20452 arg1Of TCT,GTA
R14013 T20457 T20453 arg1Of TCT,AAA
R14014 T20457 T20454 arg1Of TCT,TAT
R14015 T20457 T20455 arg1Of TCT,TTA
R14016 T20457 T20456 arg1Of TCT,CTA
R14017 T20458 T20447 arg3Of ),(
R14018 T20460 T20445 arg2Of sites,and
R14019 T20460 T20446 arg1Of sites,NFATP2/3
R14020 T20460 T20459 arg1Of sites,binding
R14021 T20460 T20461 arg1Of sites,within
R14022 T20464 T20461 arg2Of promoter,within
R14023 T20464 T20462 arg1Of promoter,the
R14024 T20464 T20463 arg1Of promoter,IL-1RA
R14025 T20466 T20465 arg1Of RNA,PBMC
R14026 T20466 T20467 arg1Of RNA,was
R14027 T20466 T20468 arg2Of RNA,isolated
R14028 T20468 T20467 arg2Of isolated,was
R14029 T20468 T20469 arg1Of isolated,with
R14030 T20468 T20473 arg1Of isolated,(
R14031 T20468 T20478 arg1Of isolated,and
R14032 T20472 T20469 arg2Of kit,with
R14033 T20472 T20470 arg1Of kit,an
R14034 T20472 T20471 arg1Of kit,RNeasy
R14035 T20474 T20473 arg2Of Qiagen,(
R14036 T20474 T20475 arg1Of Qiagen,","
R14037 T20476 T20475 arg2Of Germany,","
R14038 T20477 T20473 arg3Of ),(
R14039 T20478 T20511 arg1Of and,(
R14040 T20478 T20514 arg1Of and,(
R14041 T20478 T20555 arg1Of and,following
R14042 T20480 T20479 arg1Of RNA,messenger
R14043 T20480 T20481 arg1Of RNA,was
R14044 T20480 T20482 arg2Of RNA,transcribed
R14045 T20480 T20495 arg2Of RNA,analysed
R14046 T20482 T20483 arg1Of transcribed,into
R14047 T20482 T20494 arg1Of transcribed,and
R14048 T20484 T20483 arg2Of cDNA,into
R14049 T20484 T20485 arg1Of cDNA,with
R14050 T20489 T20487 arg1Of Strand,Ready
R14051 T20489 T20488 arg1Of Strand,First
R14052 T20490 T20485 arg2Of kit,with
R14053 T20490 T20486 arg1Of kit,Reactin
R14054 T20490 T20489 arg1Of kit,Strand
R14055 T20490 T20491 arg1Of kit,(
R14056 T20492 T20491 arg2Of Biomol,(
R14057 T20493 T20491 arg3Of ),(
R14058 T20494 T20478 arg2Of and,and
R14059 T20494 T20481 arg2Of and,was
R14060 T20495 T20494 arg2Of analysed,and
R14061 T20495 T20496 arg1Of analysed,for
R14062 T20497 T20498 arg1Of IL-1RA,and
R14063 T20499 T20498 arg2Of GAPDH,and
R14064 T20500 T20496 arg2Of transcripts,for
R14065 T20500 T20497 arg1Of transcripts,IL-1RA
R14066 T20500 T20499 arg1Of transcripts,GAPDH
R14067 T20502 T20495 arg1Of PCR,analysed
R14068 T20502 T20501 arg2Of PCR,by
R14069 T20502 T20503 arg1Of PCR,with
R14070 T20510 T20503 arg2Of mastermix,with
R14071 T20510 T20504 arg1Of mastermix,a
R14072 T20510 T20505 arg1Of mastermix,HotStart
R14073 T20510 T20506 arg1Of mastermix,""""
R14074 T20510 T20507 arg1Of mastermix,sweet
R14075 T20510 T20508 arg1Of mastermix,""""
R14076 T20510 T20509 arg1Of mastermix,PCR
R14077 T20512 T20511 arg2Of Biomol,(
R14078 T20513 T20511 arg3Of ),(
R14079 T20515 T20516 arg1Of 95°C,","
R14080 T20516 T20519 arg1Of ",",","
R14081 T20518 T20516 arg2Of sec,","
R14082 T20518 T20517 arg1Of sec,30
R14083 T20519 T20521 arg1Of ",",","
R14084 T20520 T20519 arg2Of 55°C,","
R14085 T20521 T20524 arg1Of ",",","
R14086 T20523 T20521 arg2Of sec,","
R14087 T20523 T20522 arg1Of sec,30
R14088 T20524 T20526 arg1Of ",",","
R14089 T20525 T20524 arg2Of 72°C,","
R14090 T20526 T20529 arg1Of ",",","
R14091 T20528 T20526 arg2Of sec,","
R14092 T20528 T20527 arg1Of sec,30
R14093 T20529 T20539 arg1Of ",",","
R14094 T20531 T20529 arg2Of cycles,","
R14095 T20531 T20530 arg1Of cycles,25
R14096 T20531 T20532 arg1Of cycles,for
R14097 T20533 T20534 arg1Of IL-1RA,and
R14098 T20534 T20532 arg2Of and,for
R14099 T20534 T20536 arg1Of and,for
R14100 T20535 T20534 arg2Of 94°C,and
R14101 T20538 T20536 arg2Of sec,for
R14102 T20538 T20537 arg1Of sec,30
R14103 T20539 T20549 arg1Of ",",","
R14104 T20540 T20539 arg2Of 50°C,","
R14105 T20540 T20541 arg1Of 50°C,for
R14106 T20543 T20544 arg1Of sec,and
R14107 T20544 T20541 arg2Of and,for
R14108 T20544 T20542 arg1Of and,30
R14109 T20544 T20546 arg1Of and,for
R14110 T20545 T20544 arg2Of 72°C,and
R14111 T20548 T20546 arg2Of sec,for
R14112 T20548 T20547 arg1Of sec,45
R14113 T20549 T20514 arg2Of ",",(
R14114 T20551 T20549 arg2Of cycles,","
R14115 T20551 T20550 arg1Of cycles,25
R14116 T20551 T20552 arg1Of cycles,for
R14117 T20553 T20552 arg2Of GAPDH,for
R14118 T20554 T20514 arg3Of ),(
R14119 T20558 T20555 arg2Of incubation,following
R14120 T20558 T20556 arg1Of incubation,18
R14121 T20558 T20557 arg1Of incubation,h
R14122 T20558 T20559 arg1Of incubation,with
R14123 T20560 T20559 arg2Of GP,with
R14124 T20562 T20561 arg1Of protein,IL-1RA
R14125 T20562 T20563 arg1Of protein,was
R14126 T20562 T20564 arg2Of protein,measured
R14127 T20564 T20563 arg2Of measured,was
R14128 T20564 T20565 arg1Of measured,in
R14129 T20564 T20570 arg1Of measured,after
R14130 T20567 T20565 arg2Of supernatant,in
R14131 T20567 T20566 arg1Of supernatant,the
R14132 T20567 T20568 arg1Of supernatant,by
R14133 T20569 T20568 arg2Of ELISA,by
R14134 T20571 T20572 arg1Of 24,h
R14135 T20573 T20570 arg2Of incubation,after
R14136 T20573 T20571 arg1Of incubation,24
R14137 T20573 T20574 arg1Of incubation,with
R14138 T20575 T20574 arg2Of GP,with
R14430 T21016 T21015 arg1Of analysis,Statistical
R14431 T21019 T21017 arg1Of test,The
R14432 T21019 T21018 arg1Of test,Kolmogorow-Smirnov
R14433 T21019 T21020 arg1Of test,was
R14434 T21019 T21021 arg2Of test,used
R14435 T21021 T21020 arg2Of used,was
R14436 T21023 T21021 arg3Of evaluate,used
R14437 T21023 T21022 arg1Of evaluate,to
R14438 T21023 T21024 arg1Of evaluate,","
R14439 T21027 T21026 arg1Of amounts,cytokine
R14440 T21027 T21028 arg1Of amounts,and
R14441 T21028 T21031 arg1Of and,to
R14442 T21028 T21034 arg1Of and,were
R14443 T21028 T21036 arg2Of and,distributed
R14444 T21030 T21028 arg2Of activities,and
R14445 T21030 T21029 arg1Of activities,binding
R14446 T21033 T21031 arg2Of factors,to
R14447 T21033 T21032 arg1Of factors,transcription
R14448 T21036 T21023 arg2Of distributed,evaluate
R14449 T21036 T21025 arg1Of distributed,whether
R14450 T21036 T21034 arg2Of distributed,were
R14451 T21036 T21035 arg1Of distributed,normally
R14452 T21038 T21037 arg1Of coefficients,Correlation
R14453 T21038 T21039 arg1Of coefficients,and
R14454 T21039 T21042 arg1Of and,were
R14455 T21039 T21043 arg2Of and,analyzed
R14456 T21041 T21039 arg2Of significances,and
R14457 T21041 T21040 arg1Of significances,corresponding
R14458 T21043 T21042 arg2Of analyzed,were
R14459 T21047 T21045 arg1Of test,the
R14460 T21047 T21046 arg1Of test,Pearson
R14461 T21047 T21048 arg1Of test,(
R14462 T21047 T21052 arg1Of test,or
R14463 T21050 T21048 arg2Of distribution,(
R14464 T21050 T21049 arg1Of distribution,normal
R14465 T21051 T21048 arg3Of ),(
R14466 T21052 T21043 arg1Of or,analyzed
R14467 T21052 T21044 arg2Of or,by
R14468 T21055 T21052 arg2Of test,or
R14469 T21055 T21053 arg1Of test,the
R14470 T21055 T21054 arg1Of test,Spearman
R14471 T21055 T21056 arg1Of test,(
R14472 T21058 T21056 arg2Of distribution,(
R14473 T21058 T21057 arg1Of distribution,non-normal
R14474 T21059 T21056 arg3Of ),(
R14475 T21061 T21060 arg1Of compare,To
R14476 T21061 T21064 arg1Of compare,to
R14477 T21063 T21061 arg2Of data,compare
R14478 T21063 T21062 arg1Of data,experimental
R14479 T21067 T21064 arg2Of value,to
R14480 T21067 T21065 arg1Of value,a
R14481 T21067 T21066 arg1Of value,theoretical
R14482 T21067 T21068 arg1Of value,(
R14483 T21069 T21068 arg2Of for,(
R14484 T21071 T21070 arg1Of GP,example
R14485 T21071 T21072 arg1Of GP,+
R14486 T21073 T21069 arg2Of TSST-1,for
R14487 T21073 T21071 arg1Of TSST-1,GP
R14488 T21073 T21074 arg1Of TSST-1,vs
R14489 T21076 T21074 arg2Of calc.,vs
R14490 T21076 T21075 arg1Of calc.,GP/TSST-1
R14491 T21077 T21068 arg3Of ),(
R14492 T21078 T21061 arg1Of we,compare
R14493 T21078 T21079 arg1Of we,calculated
R14494 T21078 T21087 arg1Of we,added
R14495 T21079 T21086 arg1Of calculated,and
R14496 T21081 T21079 arg2Of sum,calculated
R14497 T21081 T21080 arg1Of sum,the
R14498 T21081 T21082 arg1Of sum,of
R14499 T21085 T21082 arg2Of effects,of
R14500 T21085 T21083 arg1Of effects,the
R14501 T21085 T21084 arg1Of effects,individual
R14502 T21086 T21060 modOf and,To
R14503 T21087 T21086 arg2Of added,and
R14504 T21087 T21090 arg1Of added,into
R14505 T21089 T21087 arg2Of values,added
R14506 T21089 T21088 arg1Of values,these
R14507 T21092 T21091 arg1Of 1B,Fig.
R14508 T21092 T21093 arg1Of 1B,","
R14509 T21093 T21095 arg1Of ",",","
R14510 T21094 T21093 arg2Of 3,","
R14511 T21095 T21097 arg1Of ",",","
R14512 T21096 T21095 arg2Of 4,","
R14513 T21097 T21100 arg1Of ",",and
R14514 T21098 T21097 arg2Of 5,","
R14515 T21100 T21090 arg2Of and,into
R14516 T21100 T21099 arg1Of and,","
R14517 T21101 T21100 arg2Of 6,and
R14518 T21102 T21103 arg1Of Significances,of
R14519 T21102 T21134 arg1Of Significances,were
R14520 T21102 T21135 arg2Of Significances,analysed
R14521 T21104 T21103 arg2Of differences,of
R14522 T21104 T21105 arg1Of differences,between
R14523 T21104 T21118 arg1Of differences,between
R14524 T21104 T21124 arg1Of differences,(
R14525 T21105 T21117 arg1Of between,as
R14526 T21107 T21105 arg2Of data,between
R14527 T21107 T21106 arg1Of data,experimental
R14528 T21107 T21108 arg1Of data,(
R14529 T21109 T21108 arg2Of for,(
R14530 T21111 T21109 arg2Of GP,for
R14531 T21111 T21110 arg1Of GP,example
R14532 T21111 T21112 arg1Of GP,vs
R14533 T21113 T21112 arg2Of LPS,vs
R14534 T21114 T21108 arg3Of ),(
R14535 T21117 T21115 arg1Of as,as
R14536 T21117 T21116 arg1Of as,well
R14537 T21118 T21117 arg2Of between,as
R14538 T21120 T21119 arg1Of data,experimental
R14539 T21120 T21121 arg1Of data,and
R14540 T21121 T21118 arg2Of and,between
R14541 T21123 T21121 arg2Of values,and
R14542 T21123 T21122 arg1Of values,theoretical
R14543 T21125 T21124 arg2Of for,(
R14544 T21127 T21126 arg1Of GP,example
R14545 T21127 T21128 arg1Of GP,+
R14546 T21129 T21125 arg2Of TSST-1,for
R14547 T21129 T21127 arg1Of TSST-1,GP
R14548 T21129 T21130 arg1Of TSST-1,vs
R14549 T21132 T21130 arg2Of calc.,vs
R14550 T21132 T21131 arg1Of calc.,GP/TSST-1
R14551 T21133 T21124 arg3Of ),(
R14552 T21135 T21134 arg2Of analysed,were
R14553 T21135 T21136 modOf analysed,using
R14554 T21135 T21154 arg1Of analysed,","
R14555 T21135 T21155 arg1Of analysed,(
R14556 T21138 T21137 arg1Of Student,the
R14557 T21138 T21139 arg2Of Student,'s
R14558 T21140 T21139 arg1Of t-test,'s
R14559 T21140 T21141 arg1Of t-test,(
R14560 T21140 T21145 arg1Of t-test,or
R14561 T21143 T21141 arg2Of distribution,(
R14562 T21143 T21142 arg1Of distribution,normal
R14563 T21144 T21141 arg3Of ),(
R14564 T21145 T21136 arg2Of or,using
R14565 T21148 T21147 arg1Of Signed-Rank,Wilcoxon
R14566 T21149 T21145 arg2Of test,or
R14567 T21149 T21146 arg1Of test,the
R14568 T21149 T21148 arg1Of test,Signed-Rank
R14569 T21149 T21150 arg1Of test,(
R14570 T21152 T21150 arg2Of distribution,(
R14571 T21152 T21151 arg1Of distribution,non-normal
R14572 T21153 T21150 arg3Of ),(
R14573 T21156 T21157 arg1Of SPSS,for
R14574 T21156 T21159 arg1Of SPSS,;
R14575 T21158 T21157 arg2Of Windows,for
R14576 T21159 T21155 arg2Of ;,(
R14577 T21162 T21159 arg2Of Software,;
R14578 T21162 T21160 arg1Of Software,SPSS
R14579 T21162 T21161 arg1Of Software,Science
R14580 T21162 T21163 arg1Of Software,","
R14581 T21164 T21163 arg2Of Erkrath,","
R14582 T21164 T21165 arg1Of Erkrath,","
R14583 T21166 T21165 arg2Of Germany,","
R14584 T21167 T21155 arg3Of ),(
R13188 T19275 T19276 arg1Of Immuno,(
R13189 T19277 T19276 arg2Of Dot,(
R13190 T19278 T19276 arg3Of ),(
R13191 T19279 T19275 arg1Of blot,Immuno
R13192 T19282 T19280 arg1Of proteins,The
R13193 T19282 T19281 arg1Of proteins,nuclear
R13194 T19282 T19283 arg1Of proteins,were
R13195 T19282 T19284 arg2Of proteins,blotted
R13196 T19284 T19283 arg2Of blotted,were
R13197 T19284 T19285 arg1Of blotted,onto

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T19768 5373-5377 Protein denotes IL-1
T19767 5329-5333 Protein denotes IL-2
T19766 5259-5265 Protein denotes TGFβ 1
T19765 5187-5191 Protein denotes IL-8
T19764 5176-5182 Protein denotes IL-1RA
T19763 5101-5105 Protein denotes IL-6
T19762 5092-5096 Protein denotes IL-1
T19761 5086-5090 Protein denotes TNFα
T19760 5080-5084 Protein denotes IFNγ
T18800 3537-3542 Protein denotes Oct-1
T18799 3431-3433 Protein denotes 50
T18798 3427-3430 Protein denotes p65
T18289 2559-2563 Protein denotes NFAT
T18288 2448-2452 Protein denotes TNFα
T18287 2390-2394 Protein denotes IFNγ
T18286 1920-1924 Protein denotes IL-6
T18285 1911-1915 Protein denotes IFNγ
T18284 1905-1909 Protein denotes IL-8
T18283 1899-1903 Protein denotes TNFα
T19219 4271-4277 Protein denotes NFATc1
T19218 4243-4249 Protein denotes NFATc2
T19217 4217-4222 Protein denotes c-rel
T19216 4158-4161 Protein denotes p52
T19215 4132-4135 Protein denotes p65
T19214 4104-4107 Protein denotes p50
T19213 3998-4003 Protein denotes c-Rel
T19212 3975-3978 Protein denotes p52
T19211 3717-3721 Protein denotes NFAT
T20293 6963-6969 Protein denotes IL-1RA
T20292 6713-6718 Protein denotes GAPDH
T20291 6702-6708 Protein denotes IL-1RA
T20290 6520-6526 Protein denotes IL-1RA
T20289 6451-6452 Protein denotes 3
T20288 6444-6450 Protein denotes NFATP2
T20287 6391-6397 Protein denotes NFIL-6
T20286 5901-5907 Protein denotes IL-1RA
T21003 7751-7757 Protein denotes TSST-1
T21002 7738-7744 Protein denotes TSST-1
T21001 7466-7472 Protein denotes TSST-1
T21000 7453-7459 Protein denotes TSST-1
T17947 1733-1739 Protein denotes TSST-1
T17946 1485-1491 Protein denotes TSST-1

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T18480 1905-1909 http://www.uniprot.org/uniprot/P10145 denotes IL-8
T18479 2448-2452 http://www.uniprot.org/uniprot/P01375 denotes TNFα
T18478 1899-1903 http://www.uniprot.org/uniprot/P01375 denotes TNFα
T18481 1920-1924 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T20582 6921-6926 http://www.uniprot.org/uniprot/P04406 denotes GAPDH
T20581 6713-6718 http://www.uniprot.org/uniprot/P04406 denotes GAPDH
T20580 6963-6969 http://www.uniprot.org/uniprot/P18510 denotes IL-1RA
T20579 6842-6848 http://www.uniprot.org/uniprot/P18510 denotes IL-1RA
T20578 6702-6708 http://www.uniprot.org/uniprot/P18510 denotes IL-1RA
T20577 6520-6526 http://www.uniprot.org/uniprot/P18510 denotes IL-1RA
T20576 5901-5907 http://www.uniprot.org/uniprot/P18510 denotes IL-1RA
T19976 5329-5333 http://www.uniprot.org/uniprot/P60568 denotes IL-2
T19975 5187-5191 http://www.uniprot.org/uniprot/P10145 denotes IL-8
T19974 5176-5182 http://www.uniprot.org/uniprot/P18510 denotes IL-1RA
T19973 5101-5105 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T19972 5086-5090 http://www.uniprot.org/uniprot/P01375 denotes TNFα
T19367 4132-4135 http://www.uniprot.org/uniprot/P21579 denotes p65
T19366 4132-4135 http://www.uniprot.org/uniprot/Q04206 denotes p65
T19365 4104-4107 http://www.uniprot.org/uniprot/P19838 denotes p50
T19364 3998-4003 http://www.uniprot.org/uniprot/Q04864 denotes c-Rel
T19363 4158-4161 http://www.uniprot.org/uniprot/Q00653 denotes p52
T19362 3975-3978 http://www.uniprot.org/uniprot/Q00653 denotes p52
T21171 7751-7757 http://www.uniprot.org/uniprot/P06886 denotes TSST-1
T21170 7738-7744 http://www.uniprot.org/uniprot/P06886 denotes TSST-1
T21169 7466-7472 http://www.uniprot.org/uniprot/P06886 denotes TSST-1
T21168 7453-7459 http://www.uniprot.org/uniprot/P06886 denotes TSST-1
T18965 3427-3430 http://www.uniprot.org/uniprot/P21579 denotes p65
T18964 3427-3430 http://www.uniprot.org/uniprot/Q04206 denotes p65
T18077 1733-1739 http://www.uniprot.org/uniprot/P06886 denotes TSST-1
T18076 1485-1491 http://www.uniprot.org/uniprot/P06886 denotes TSST-1

UBERON-AE

Id Subject Object Predicate Lexical cue
T17617 1008-1013 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T17616 484-489 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood

GO-BP

Id Subject Object Predicate Lexical cue
T20272 6458-6461 http://purl.obolibrary.org/obo/GO_0033968 denotes GCA
T20271 5872-5885 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T20270 5810-5823 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T20269 5796-5831 http://purl.obolibrary.org/obo/GO_0032792 denotes Inhibition of transcription factors
T20268 5796-5831 http://purl.obolibrary.org/obo/GO_0043433 denotes Inhibition of transcription factors
T20267 5796-5831 http://purl.obolibrary.org/obo/GO_1901484 denotes Inhibition of transcription factors
T19201 4889-4891 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T19200 4728-4739 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T18272 2171-2174 http://purl.obolibrary.org/obo/GO_0051731 denotes PNK
T20995 7173-7186 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T20994 7159-7194 http://purl.obolibrary.org/obo/GO_0051090 denotes activities to transcription factors
T20993 7159-7194 http://purl.obolibrary.org/obo/GO_0006352 denotes activities to transcription factors
T20992 7159-7194 http://purl.obolibrary.org/obo/GO_0043433 denotes activities to transcription factors
T20991 7159-7194 http://purl.obolibrary.org/obo/GO_0034246 denotes activities to transcription factors
T20990 7159-7194 http://purl.obolibrary.org/obo/GO_0003700 denotes activities to transcription factors
T20989 7159-7194 http://purl.obolibrary.org/obo/GO_0001071 denotes activities to transcription factors
T20988 7159-7194 http://purl.obolibrary.org/obo/GO_0001070 denotes activities to transcription factors
T20987 7159-7194 http://purl.obolibrary.org/obo/GO_0000988 denotes activities to transcription factors
T20986 7159-7194 http://purl.obolibrary.org/obo/GO_0051091 denotes activities to transcription factors
T17943 1204-1217 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription

GO-MF

Id Subject Object Predicate Lexical cue
T20300 6458-6461 http://purl.obolibrary.org/obo/GO_0033968 denotes GCA
T20299 5861-5893 http://purl.obolibrary.org/obo/GO_0070491 denotes binding of transcription factors
T20298 5861-5893 http://purl.obolibrary.org/obo/GO_0043425 denotes binding of transcription factors
T20297 5861-5893 http://purl.obolibrary.org/obo/GO_0033613 denotes binding of transcription factors
T19773 5329-5333 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T19772 5187-5191 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T19771 5101-5105 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T19770 5373-5377 http://purl.obolibrary.org/obo/GO_0005149 denotes IL-1
T19769 5092-5096 http://purl.obolibrary.org/obo/GO_0005149 denotes IL-1
T19240 4889-4891 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T19239 4728-4739 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T19238 4761-4769 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T19237 4642-4652 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T19236 4339-4349 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T19235 4071-4081 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T19234 3870-3880 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T19233 3675-3691 http://purl.obolibrary.org/obo/GO_0005515 denotes binding proteins
T19232 3675-3682 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T19231 3671-3682 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T21009 7159-7194 http://purl.obolibrary.org/obo/GO_0034246 denotes activities to transcription factors
T21008 7159-7194 http://purl.obolibrary.org/obo/GO_0003700 denotes activities to transcription factors
T21007 7159-7194 http://purl.obolibrary.org/obo/GO_0001071 denotes activities to transcription factors
T21006 7159-7194 http://purl.obolibrary.org/obo/GO_0001070 denotes activities to transcription factors
T21005 7159-7194 http://purl.obolibrary.org/obo/GO_0000988 denotes activities to transcription factors
T21004 7151-7158 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T20296 5861-5893 http://purl.obolibrary.org/obo/GO_0008134 denotes binding of transcription factors
T20295 6495-6502 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T20294 5861-5868 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T18295 2548-2563 http://purl.obolibrary.org/obo/GO_0051525 denotes binding of NFAT
T18294 2548-2555 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T18293 2272-2279 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T18292 2171-2174 http://purl.obolibrary.org/obo/GO_0051731 denotes PNK
T18291 1920-1924 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T18290 1905-1909 http://purl.obolibrary.org/obo/GO_0005153 denotes IL-8
T17466 403-418 http://purl.obolibrary.org/obo/GO_0001872 denotes β-1→3-D-glucans
T18805 3434-3442 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T18804 3618-3625 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T18803 3375-3382 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T18802 3232-3239 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T18801 3156-3163 http://purl.obolibrary.org/obo/GO_0005488 denotes binding

GO-CC

Id Subject Object Predicate Lexical cue
T19774 4938-4942 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T19253 4510-4518 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T19252 4314-4318 http://purl.obolibrary.org/obo/GO_0071735 denotes IgG1
T19251 4196-4200 http://purl.obolibrary.org/obo/GO_0071735 denotes IgG1
T19250 4761-4769 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T19249 4642-4652 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T19248 4339-4349 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T19247 4071-4081 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T19246 3870-3880 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T19245 4761-4769 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T19244 4642-4652 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T19243 4339-4349 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T19242 4071-4081 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T19241 3870-3880 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T18807 3434-3442 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T18806 3434-3442 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T17950 1551-1556 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17949 1479-1484 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17948 1450-1455 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17623 868-873 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17622 792-797 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T20985 7581-7948 Sentence denotes Significances of differences between experimental data (for example GP vs LPS) as well as between experimental data and theoretical values (for example GP + TSST-1 vs GP/TSST-1 calc.) were analysed using the Student's t-test (normal distribution) or the Wilcoxon Signed-Rank test (non-normal distribution), (SPSS for Windows; SPSS Science Software, Erkrath, Germany).
T20984 7383-7580 Sentence denotes To compare experimental data to a theoretical value (for example GP + TSST-1 vs GP/TSST-1 calc.) we calculated the sum of the individual effects and added these values into Fig. 1B, 3, 4, 5, and 6.
T20983 7222-7382 Sentence denotes Correlation coefficients and corresponding significances were analyzed by the Pearson test (normal distribution) or the Spearman test (non-normal distribution).
T20982 7072-7221 Sentence denotes The Kolmogorow-Smirnov test was used to evaluate, whether cytokine amounts and binding activities to transcription factors were normally distributed.
T20981 7051-7071 Sentence denotes Statistical analysis
T20266 6963-7049 Sentence denotes IL-1RA protein was measured in the supernatant by ELISA after 24 h incubation with GP.
T20265 6537-6962 Sentence denotes PBMC RNA was isolated with an RNeasy kit (Qiagen, Germany) and messenger RNA was transcribed into cDNA with Reactin Ready First Strand kit (Biomol) and analysed for IL-1RA and GAPDH transcripts by PCR with a HotStart "sweet" PCR mastermix (Biomol) (95°C, 30 sec, 55°C, 30 sec, 72°C, 30 sec, 25 cycles for IL-1RA and 94°C for 30 sec, 50°C for 30 sec and 72°C for 45 sec, 25 cycles for GAPDH) following 18 h incubation with GP.
T20264 6284-6536 Sentence denotes Band shifts were determined after 1 h incubation with GP as described before, including mutated oligos for NFIL-6 (TTA CAA CAG TGG ATT GCG ACA CTT AGT GGG) and NFATP2/3 (GGC GCA GAA AAG GTA AAA TAT TTA CTA TCT) binding sites within the IL-1RA promoter.
T20263 6033-6283 Sentence denotes 50 μg/ml of CAPE (caffeic-3,4-dihydroxycinnamic-acid-phenyl-ester), (Biomol, Hamburg, Germany); 400 ng/ml cyclosporin A (Calbiochem-Merck Biosciences, Bad Soden, Germany); 10 μg/ml cycloheximide (Santa Cruz Biotechnology) prior to incubation with GP.
T20262 5832-6032 Sentence denotes To inhibit translocation and binding of transcription factors to the IL-1RA promoter, PBMC were incubated for 1 h with each of the following substances (concentrations were based upon 50% inhibition):
T20261 5796-5831 Sentence denotes Inhibition of transcription factors
T19750 5679-5794 Sentence denotes Only stimulation of PBMC observed in donors showing no spontaneous cytokine release was included in the statistics.
T19749 5613-5678 Sentence denotes Cytokine amounts were all within the range of the standard curve.
T19748 5489-5612 Sentence denotes All cytokines were quantified using an ELISA plate reader (Anthos Labotec, Salzburg, Austria or Microplate Reader, BioRad).
T19747 5417-5488 Sentence denotes Samples were diluted in the same buffer as that used for the standards.
T19746 5311-5416 Sentence denotes Quantification of IL-2 as an indicator for the bioactivity of IL-1 was done as previously described [46].
T19745 5080-5310 Sentence denotes IFNγ, TNFα, IL-1 and IL-6 were determined using an ELISA from Bender Systems (Vienna, Austria), IL-1RA and IL-8 by an ELISA from R&D Systems (Wiesbaden-Nordenstadt, Germany), and TGFβ 1 using an ELISA as previously described [57].
T19744 4922-5079 Sentence denotes Supernatants of cell cultures were harvested 3, 6, 9, 12, 18, 24, 30, 36 and 48 hours after stimulation and stored at -80 °C until used and thawed only once.
T19743 4900-4921 Sentence denotes Cytokine measurements
T19199 4793-4898 Sentence denotes The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc. Burlingame, CA, USA).
T19198 4530-4792 Sentence denotes After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA).
T19197 4454-4529 Sentence denotes The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad).
T19196 3646-4453 Sentence denotes To determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher & Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022.
T19195 3628-3645 Sentence denotes Immuno (Dot) blot
T18794 3476-3626 Sentence denotes To exclude non-specific reactions, a 30-fold molar excess of Oct-1 DNA (unrelated DNA, Table 1) was used, which did not compete with specific binding.
T18793 3356-3475 Sentence denotes For specificity of binding, supershift assays for NFκB with a combined p65/50 antibody were performed (data not shown).
T18792 3261-3355 Sentence denotes For such experiments, an excess (5–50×) of unlabeled oligos was added to the nuclear extracts.
T18791 3065-3260 Sentence denotes Competition experiments with labeled and unlabeled mutated oligos of the cytokine promoter binding sites (see above) were carried out in order to prevent non-specific binding to nuclear proteins.
T18790 2984-3064 Sentence denotes The intensity of shifted bands was normalized to bands of unstimulated controls.
T18789 2833-2983 Sentence denotes Nuclear extracts of purified PBMCs were prepared according to a technique described by Trede et al. [27] and modified for our experimental setup [56].
T18788 2752-2832 Sentence denotes Preparation of nuclear extracts and electrophoretic mobility shift assays (EMSA)
T17942 1636-1776 Sentence denotes In costimulatory experiments, PBMC were supplemented simultaneously with a combination of GP and TSST-1 or with a combination of GP and LPS.
T17941 1357-1635 Sentence denotes Cultures were stimulated for 1 h (EMSA) or from 0 h to 48 h (ELISA) with 1 or 100 μg per 106 cells of GP, with 250 ng/106 cells TSST-1 (Toxin Technologies, Sarasota, FL, USA) or with 250 ng/106 cells wild-type LPS from Escherichia coli serotype 0111:B4 (Sigma, Munich, Germany).
T18271 2597-2750 Sentence denotes Afterwards, the ds oligos were purified via gel filtration using Probe Quant G-50 Micro Columns (Pharmacia) according to the manufacturer's instructions.
T18270 2358-2596 Sentence denotes Mutations in the P2 site of the IFNγ promoter [29] and in the κ consensus sequence of the TNFα promoter [55] have been reported to interfere with sequences, which seem to be crucial for the binding of NFAT and NFκB proteins, respectively.
T18269 2260-2357 Sentence denotes To test the binding specificity of the oligos, mutated oligos were used as an additional control.
T18268 1825-2259 Sentence denotes The complementary double-stranded (ds) oligonucleotides (oligos) from the TNFα, IL-8, IFNγ and IL-6 promoters were synthesised from single stranded (ss) oligos (illustrated in Table 1), (TIB Molbiol, Berlin, Germany) and 32P-labeled with 5'γ-P-ATP (3,000 Ci/mmol, Amersham, Braunschweig, Germany) using the Ready-To-Go-Polynucleotide-Kinase Kit (PNK Kit, Pharmacia LKB, Freiburg, Germany) according to the manufacturer's instructions.
T18267 1778-1824 Sentence denotes Oligonucleotides (Oligos) and 5'-32-P-Labeling
T17620 716-1162 Sentence denotes Following isolation, PBMC were cultured at a final concentration of 3 × 106 cells/ml (for ELISA measurements) or 5 × 106/ml (for gel shifts) or 1 × 106 cells/ml (for flow cytometry) in RPMI 1640 medium (BioWhittaker, Heidelberg, Germany, LPS-free), containing 10% heat-inactivated fetal calf serum (FCS, low-LPS, Invitrogen, Karlsruhe, Germany), 1% penicillin (10000 U/ml), 1% streptomycin (10000 μg/ml), and 1% 200 mM L-glutamine (BioWhittaker).
T17619 445-715 Sentence denotes Buffy coats were obtained from healthy blood donors in compliance with the Helsinki declaration and with the approval of the ethics committee of the University of Luebeck. The buffy coats were separated over a Ficoll-Hypaque (Biochrom, Berlin, Germany) gradient [54,55].
T17618 421-444 Sentence denotes Isolation of human PBMC
T17940 1187-1356 Sentence denotes For induction of transcription factors and cytokines, PBMC were cultured in a volume of 4 ml in sterile pyrogen-free 6-well culture plates (Falcon, Heidelberg, Germany).
T17939 1164-1186 Sentence denotes Stimulation conditions
T17464 192-419 Sentence denotes GP was dissolved in aqueous media, filter-sterilized (0.2 μm) and screened for endotoxin contamination with the Endospecy assay (Seigakaku, Tokyo, Japan), which is specific for endotoxin but does not respond to β-1→3-D-glucans.
T17463 102-191 Sentence denotes The physicochemical characteristics of GP were determined as reported previously [20,26].
T17462 19-101 Sentence denotes Water soluble phosphorylated β-1→3-D-glucan (GP) was prepared as done before [20].
T17461 9-18 Sentence denotes Materials
T226 0-7 Sentence denotes Methods
T227 9-18 Sentence denotes Materials
T228 19-101 Sentence denotes Water soluble phosphorylated β-1→3-D-glucan (GP) was prepared as done before [20].
T229 102-191 Sentence denotes The physicochemical characteristics of GP were determined as reported previously [20,26].
T230 192-419 Sentence denotes GP was dissolved in aqueous media, filter-sterilized (0.2 μm) and screened for endotoxin contamination with the Endospecy assay (Seigakaku, Tokyo, Japan), which is specific for endotoxin but does not respond to β-1→3-D-glucans.
T231 421-444 Sentence denotes Isolation of human PBMC
T232 445-616 Sentence denotes Buffy coats were obtained from healthy blood donors in compliance with the Helsinki declaration and with the approval of the ethics committee of the University of Luebeck.
T233 617-715 Sentence denotes The buffy coats were separated over a Ficoll-Hypaque (Biochrom, Berlin, Germany) gradient [54,55].
T234 716-1162 Sentence denotes Following isolation, PBMC were cultured at a final concentration of 3 × 106 cells/ml (for ELISA measurements) or 5 × 106/ml (for gel shifts) or 1 × 106 cells/ml (for flow cytometry) in RPMI 1640 medium (BioWhittaker, Heidelberg, Germany, LPS-free), containing 10% heat-inactivated fetal calf serum (FCS, low-LPS, Invitrogen, Karlsruhe, Germany), 1% penicillin (10000 U/ml), 1% streptomycin (10000 μg/ml), and 1% 200 mM L-glutamine (BioWhittaker).
T235 1164-1186 Sentence denotes Stimulation conditions
T236 1187-1356 Sentence denotes For induction of transcription factors and cytokines, PBMC were cultured in a volume of 4 ml in sterile pyrogen-free 6-well culture plates (Falcon, Heidelberg, Germany).
T237 1357-1635 Sentence denotes Cultures were stimulated for 1 h (EMSA) or from 0 h to 48 h (ELISA) with 1 or 100 μg per 106 cells of GP, with 250 ng/106 cells TSST-1 (Toxin Technologies, Sarasota, FL, USA) or with 250 ng/106 cells wild-type LPS from Escherichia coli serotype 0111:B4 (Sigma, Munich, Germany).
T238 1636-1776 Sentence denotes In costimulatory experiments, PBMC were supplemented simultaneously with a combination of GP and TSST-1 or with a combination of GP and LPS.
T239 1778-1824 Sentence denotes Oligonucleotides (Oligos) and 5'-32-P-Labeling
T240 1825-2259 Sentence denotes The complementary double-stranded (ds) oligonucleotides (oligos) from the TNFα, IL-8, IFNγ and IL-6 promoters were synthesised from single stranded (ss) oligos (illustrated in Table 1), (TIB Molbiol, Berlin, Germany) and 32P-labeled with 5'γ-P-ATP (3,000 Ci/mmol, Amersham, Braunschweig, Germany) using the Ready-To-Go-Polynucleotide-Kinase Kit (PNK Kit, Pharmacia LKB, Freiburg, Germany) according to the manufacturer's instructions.
T241 2260-2357 Sentence denotes To test the binding specificity of the oligos, mutated oligos were used as an additional control.
T242 2358-2596 Sentence denotes Mutations in the P2 site of the IFNγ promoter [29] and in the κ consensus sequence of the TNFα promoter [55] have been reported to interfere with sequences, which seem to be crucial for the binding of NFAT and NFκB proteins, respectively.
T243 2597-2750 Sentence denotes Afterwards, the ds oligos were purified via gel filtration using Probe Quant G-50 Micro Columns (Pharmacia) according to the manufacturer's instructions.
T244 2752-2832 Sentence denotes Preparation of nuclear extracts and electrophoretic mobility shift assays (EMSA)
T245 2833-2983 Sentence denotes Nuclear extracts of purified PBMCs were prepared according to a technique described by Trede et al. [27] and modified for our experimental setup [56].
T246 2984-3064 Sentence denotes The intensity of shifted bands was normalized to bands of unstimulated controls.
T247 3065-3260 Sentence denotes Competition experiments with labeled and unlabeled mutated oligos of the cytokine promoter binding sites (see above) were carried out in order to prevent non-specific binding to nuclear proteins.
T248 3261-3355 Sentence denotes For such experiments, an excess (5–50×) of unlabeled oligos was added to the nuclear extracts.
T249 3356-3475 Sentence denotes For specificity of binding, supershift assays for NFκB with a combined p65/50 antibody were performed (data not shown).
T250 3476-3626 Sentence denotes To exclude non-specific reactions, a 30-fold molar excess of Oct-1 DNA (unrelated DNA, Table 1) was used, which did not compete with specific binding.
T251 3628-3645 Sentence denotes Immuno (Dot) blot
T252 3646-4453 Sentence denotes To determine whether the DNA binding proteins were related to NFκB and NFAT, dot blots using a SRC96D SNS minifold I dot blotter (Schleicher & Schüll, Dassel, Germany) were performed with the following positive controls and antibodies (all from Santa Cruz Biotechnology, Heidelberg, Germany): positive controls (10 ng/dot): NFκB p52 (80 kD) sc-4095WB, c-Rel (61 kD) sc-4030WB; Jurkat nuclear extract, PMA-stimulated; primary antibodies (1 μg/dot): anti-NFκB p50 (NLS) sc-114, anti-NFκB p65 (A) sc-109, anti-NFκB p52 (447) sc-848, a rabbit polyclonal IgG1 antiserum, anti-c-rel (N466) sc-272, anti-NFATc2 (M-20) sc-1151, anti-NFATc1 (K-18) sc-1149, two goat polyclonal IgG1 antisera; secondary antibodies (0.8 μg/dot): goat anti-rabbit IgG AP-conjugate sc-2007, and donkey anti-goat IgG AP-conjugate sc-2022.
T253 4454-4529 Sentence denotes The nuclear proteins were blotted onto a nitrocellulose membrane (Bio-Rad).
T254 4530-4792 Sentence denotes After blocking with PBS/3% BSA (Fluka, Deisenhofen, Germany), the blot was incubated overnight with the primary antibodies diluted 1:2000 in PBS/1% BSA, washed again and incubated with the alkaline phosphatase-conjugated secondary antibody (1:500 in PBS/1% BSA).
T255 4793-4876 Sentence denotes The blot was developed using the Vectastain® staining kit (Vector Laboratories Inc.
T256 4877-4898 Sentence denotes Burlingame, CA, USA).
T257 4900-4921 Sentence denotes Cytokine measurements
T258 4922-5079 Sentence denotes Supernatants of cell cultures were harvested 3, 6, 9, 12, 18, 24, 30, 36 and 48 hours after stimulation and stored at -80 °C until used and thawed only once.
T259 5080-5310 Sentence denotes IFNγ, TNFα, IL-1 and IL-6 were determined using an ELISA from Bender Systems (Vienna, Austria), IL-1RA and IL-8 by an ELISA from R&D Systems (Wiesbaden-Nordenstadt, Germany), and TGFβ 1 using an ELISA as previously described [57].
T260 5311-5416 Sentence denotes Quantification of IL-2 as an indicator for the bioactivity of IL-1 was done as previously described [46].
T261 5417-5488 Sentence denotes Samples were diluted in the same buffer as that used for the standards.
T262 5489-5612 Sentence denotes All cytokines were quantified using an ELISA plate reader (Anthos Labotec, Salzburg, Austria or Microplate Reader, BioRad).
T263 5613-5678 Sentence denotes Cytokine amounts were all within the range of the standard curve.
T264 5679-5794 Sentence denotes Only stimulation of PBMC observed in donors showing no spontaneous cytokine release was included in the statistics.
T265 5796-5831 Sentence denotes Inhibition of transcription factors
T266 5832-6032 Sentence denotes To inhibit translocation and binding of transcription factors to the IL-1RA promoter, PBMC were incubated for 1 h with each of the following substances (concentrations were based upon 50% inhibition):
T267 6033-6283 Sentence denotes 50 μg/ml of CAPE (caffeic-3,4-dihydroxycinnamic-acid-phenyl-ester), (Biomol, Hamburg, Germany); 400 ng/ml cyclosporin A (Calbiochem-Merck Biosciences, Bad Soden, Germany); 10 μg/ml cycloheximide (Santa Cruz Biotechnology) prior to incubation with GP.
T268 6284-6536 Sentence denotes Band shifts were determined after 1 h incubation with GP as described before, including mutated oligos for NFIL-6 (TTA CAA CAG TGG ATT GCG ACA CTT AGT GGG) and NFATP2/3 (GGC GCA GAA AAG GTA AAA TAT TTA CTA TCT) binding sites within the IL-1RA promoter.
T269 6537-6962 Sentence denotes PBMC RNA was isolated with an RNeasy kit (Qiagen, Germany) and messenger RNA was transcribed into cDNA with Reactin Ready First Strand kit (Biomol) and analysed for IL-1RA and GAPDH transcripts by PCR with a HotStart "sweet" PCR mastermix (Biomol) (95°C, 30 sec, 55°C, 30 sec, 72°C, 30 sec, 25 cycles for IL-1RA and 94°C for 30 sec, 50°C for 30 sec and 72°C for 45 sec, 25 cycles for GAPDH) following 18 h incubation with GP.
T270 6963-7049 Sentence denotes IL-1RA protein was measured in the supernatant by ELISA after 24 h incubation with GP.
T271 7051-7071 Sentence denotes Statistical analysis
T272 7072-7221 Sentence denotes The Kolmogorow-Smirnov test was used to evaluate, whether cytokine amounts and binding activities to transcription factors were normally distributed.
T273 7222-7382 Sentence denotes Correlation coefficients and corresponding significances were analyzed by the Pearson test (normal distribution) or the Spearman test (non-normal distribution).
T274 7383-7580 Sentence denotes To compare experimental data to a theoretical value (for example GP + TSST-1 vs GP/TSST-1 calc.) we calculated the sum of the individual effects and added these values into Fig. 1B, 3, 4, 5, and 6.
T275 7581-7948 Sentence denotes Significances of differences between experimental data (for example GP vs LPS) as well as between experimental data and theoretical values (for example GP + TSST-1 vs GP/TSST-1 calc.) were analysed using the Student's t-test (normal distribution) or the Wilcoxon Signed-Rank test (non-normal distribution), (SPSS for Windows; SPSS Science Software, Erkrath, Germany).

ICD10

Id Subject Object Predicate Lexical cue
T17621 484-496 http://purl.bioontology.org/ontology/ICD10/Z52.0 denotes blood donors
T17465 234-244 http://purl.bioontology.org/ontology/ICD10/Z30.2 denotes sterilized

simple1

Id Subject Object Predicate Lexical cue
T20331 6963-6969 Protein denotes IL-1RA
T20330 6713-6718 Protein denotes GAPDH
T20329 6702-6708 Protein denotes IL-1RA
T20328 6520-6526 Protein denotes IL-1RA
T20327 6451-6452 Protein denotes 3
T20326 6444-6450 Protein denotes NFATP2
T20325 6391-6397 Protein denotes NFIL-6
T20324 5901-5907 Protein denotes IL-1RA
T19800 5373-5377 Protein denotes IL-1
T19799 5329-5333 Protein denotes IL-2
T19798 5259-5265 Protein denotes TGFβ 1
T19797 5187-5191 Protein denotes IL-8
T19796 5176-5182 Protein denotes IL-1RA
T19795 5101-5105 Protein denotes IL-6
T19794 5092-5096 Protein denotes IL-1
T19793 5086-5090 Protein denotes TNFα
T19792 5080-5084 Protein denotes IFNγ
T19274 4271-4277 Protein denotes NFATc1
T19273 4243-4249 Protein denotes NFATc2
T19272 4217-4222 Protein denotes c-rel
T19271 4158-4161 Protein denotes p52
T19270 4132-4135 Protein denotes p65
T19269 4104-4107 Protein denotes p50
T19268 3998-4003 Protein denotes c-Rel
T19267 3975-3978 Protein denotes p52
T19266 3717-3721 Protein denotes NFAT
T18302 2559-2563 Protein denotes NFAT
T18301 2448-2452 Protein denotes TNFα
T18300 2390-2394 Protein denotes IFNγ
T18299 1920-1924 Protein denotes IL-6
T18298 1911-1915 Protein denotes IFNγ
T18297 1905-1909 Protein denotes IL-8
T18296 1899-1903 Protein denotes TNFα
T21014 7751-7757 Protein denotes TSST-1
T21013 7738-7744 Protein denotes TSST-1
T21012 7466-7472 Protein denotes TSST-1
T21011 7453-7459 Protein denotes TSST-1
T18813 3537-3542 Protein denotes Oct-1
T18812 3431-3433 Protein denotes 50
T18811 3427-3430 Protein denotes p65
T17954 1733-1739 Protein denotes TSST-1
T17953 1485-1491 Protein denotes TSST-1

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T20956 5861-5868 Binding denotes binding
T20955 5835-5842 Negative_regulation denotes inhibit
T20954 6963-6969 Protein denotes IL-1RA
T20953 6713-6718 Protein denotes GAPDH
T20952 6702-6708 Protein denotes IL-1RA
T20951 6520-6526 Protein denotes IL-1RA
T20950 6451-6452 Protein denotes 3
T20949 6444-6450 Protein denotes NFATP2
T20948 6391-6397 Protein denotes NFIL-6
T20947 5901-5907 Protein denotes IL-1RA
T21364 7751-7757 Protein denotes TSST-1
T21363 7738-7744 Protein denotes TSST-1
T21362 7466-7472 Protein denotes TSST-1
T21361 7453-7459 Protein denotes TSST-1
T20214 5373-5377 Protein denotes IL-1
T20213 5329-5333 Protein denotes IL-2
T20212 5259-5265 Protein denotes TGFβ 1
T20211 5187-5191 Protein denotes IL-8
T20210 5176-5182 Protein denotes IL-1RA
T20209 5101-5105 Protein denotes IL-6
T20208 5092-5096 Protein denotes IL-1
T20207 5086-5090 Protein denotes TNFα
T20206 5080-5084 Protein denotes IFNγ
T19697 4271-4277 Protein denotes NFATc1
T19696 4243-4249 Protein denotes NFATc2
T19695 4217-4222 Protein denotes c-rel
T19694 4158-4161 Protein denotes p52
T19693 4132-4135 Protein denotes p65
T19692 4104-4107 Protein denotes p50
T19691 3998-4003 Protein denotes c-Rel
T19690 3975-3978 Protein denotes p52
T19689 3717-3721 Protein denotes NFAT
T19164 3527-3533 Negative_regulation denotes excess
T19163 3537-3542 Protein denotes Oct-1
T19162 3431-3433 Protein denotes 50
T19161 3427-3430 Protein denotes p65
T18764 2532-2539 Positive_regulation denotes crucial
T18763 2548-2555 Binding denotes binding
T18762 2559-2563 Protein denotes NFAT
T18761 2448-2452 Protein denotes TNFα
T18760 2390-2394 Protein denotes IFNγ
T18759 1920-1924 Protein denotes IL-6
T18758 1911-1915 Protein denotes IFNγ
T18757 1905-1909 Protein denotes IL-8
T18756 1899-1903 Protein denotes TNFα
T18230 1733-1739 Protein denotes TSST-1
T18229 1485-1491 Protein denotes TSST-1
R12861 T18762 T18763 themeOf NFAT,binding
R12862 T18763 T18764 themeOf binding,crucial
R13183 T19163 T19164 themeOf Oct-1,excess
R14428 T20947 T20956 themeOf IL-1RA,binding
R14429 T20956 T20955 themeOf binding,inhibit

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T20591 5861-5868 Binding denotes binding
T20590 6963-6969 Protein denotes IL-1RA
T20589 6713-6718 Protein denotes GAPDH
T20588 6702-6708 Protein denotes IL-1RA
T20587 6520-6526 Protein denotes IL-1RA
T20586 6451-6452 Protein denotes 3
T20585 6444-6450 Protein denotes NFATP2
T20584 6391-6397 Protein denotes NFIL-6
T20583 5901-5907 Protein denotes IL-1RA
T21175 7751-7757 Protein denotes TSST-1
T21174 7738-7744 Protein denotes TSST-1
T21173 7466-7472 Protein denotes TSST-1
T21172 7453-7459 Protein denotes TSST-1
T19985 5373-5377 Protein denotes IL-1
T19984 5329-5333 Protein denotes IL-2
T19983 5259-5265 Protein denotes TGFβ 1
T19982 5187-5191 Protein denotes IL-8
T19981 5176-5182 Protein denotes IL-1RA
T19980 5101-5105 Protein denotes IL-6
T19979 5092-5096 Protein denotes IL-1
T19978 5086-5090 Protein denotes TNFα
T19977 5080-5084 Protein denotes IFNγ
T19376 4271-4277 Protein denotes NFATc1
T19375 4243-4249 Protein denotes NFATc2
T19374 4217-4222 Protein denotes c-rel
T19373 4158-4161 Protein denotes p52
T19372 4132-4135 Protein denotes p65
T19371 4104-4107 Protein denotes p50
T19370 3998-4003 Protein denotes c-Rel
T19369 3975-3978 Protein denotes p52
T19368 3717-3721 Protein denotes NFAT
T18968 3537-3542 Protein denotes Oct-1
T18967 3431-3433 Protein denotes 50
T18966 3427-3430 Protein denotes p65
T18490 2548-2555 Binding denotes binding
T18489 2532-2539 Positive_regulation denotes crucial
T18488 2559-2563 Protein denotes NFAT
T18487 2448-2452 Protein denotes TNFα
T18486 2390-2394 Protein denotes IFNγ
T18485 1920-1924 Protein denotes IL-6
T18484 1911-1915 Protein denotes IFNγ
T18483 1905-1909 Protein denotes IL-8
T18482 1899-1903 Protein denotes TNFα
T18079 1733-1739 Protein denotes TSST-1
T18078 1485-1491 Protein denotes TSST-1
R12655 T18488 T18490 themeOf NFAT,binding
R12656 T18490 T18489 themeOf binding,crucial
R14139 T20583 T20591 themeOf IL-1RA,binding

DLUT931

Id Subject Object Predicate Lexical cue
T20601 6495-6502 Binding denotes binding
T20600 5861-5868 Binding denotes binding
T20599 6963-6969 Protein denotes IL-1RA
T20598 6713-6718 Protein denotes GAPDH
T20597 6702-6708 Protein denotes IL-1RA
T20596 6520-6526 Protein denotes IL-1RA
T20595 6451-6452 Protein denotes 3
T20594 6444-6450 Protein denotes NFATP2
T20593 6391-6397 Protein denotes NFIL-6
T20592 5901-5907 Protein denotes IL-1RA
T20003 5373-5377 Protein denotes IL-1
T20002 5329-5333 Protein denotes IL-2
T20001 5259-5265 Protein denotes TGFβ 1
T20000 5187-5191 Protein denotes IL-8
T19999 5176-5182 Protein denotes IL-1RA
T19998 5101-5105 Protein denotes IL-6
T19997 5092-5096 Protein denotes IL-1
T19996 5086-5090 Protein denotes TNFα
T19995 5080-5084 Protein denotes IFNγ
T19385 4271-4277 Protein denotes NFATc1
T19384 4243-4249 Protein denotes NFATc2
T19383 4217-4222 Protein denotes c-rel
T19382 4158-4161 Protein denotes p52
T19381 4132-4135 Protein denotes p65
T19380 4104-4107 Protein denotes p50
T19379 3998-4003 Protein denotes c-Rel
T19378 3975-3978 Protein denotes p52
T19377 3717-3721 Protein denotes NFAT
T18979 3618-3625 Binding denotes binding
T18978 3527-3533 Negative_regulation denotes excess
T18977 3537-3542 Protein denotes Oct-1
T18976 3431-3433 Protein denotes 50
T18975 3427-3430 Protein denotes p65
T18521 2532-2539 Positive_regulation denotes crucial
T18520 2548-2555 Binding denotes binding
T18519 2559-2563 Protein denotes NFAT
T18518 2448-2452 Protein denotes TNFα
T18517 2390-2394 Protein denotes IFNγ
T18516 1920-1924 Protein denotes IL-6
T18515 1911-1915 Protein denotes IFNγ
T18514 1905-1909 Protein denotes IL-8
T18513 1899-1903 Protein denotes TNFα
T18083 1733-1739 Protein denotes TSST-1
T18082 1485-1491 Protein denotes TSST-1
T21181 7466-7472 Protein denotes TSST-1
T21180 7453-7459 Protein denotes TSST-1
T21183 7751-7757 Protein denotes TSST-1
T21182 7738-7744 Protein denotes TSST-1
R12663 T18519 T18520 themeOf NFAT,binding
R12664 T18520 T18521 themeOf binding,crucial
R13019 T18977 T18978 themeOf Oct-1,excess
R13020 T18977 T18979 themeOf Oct-1,binding
R14140 T20592 T20600 themeOf IL-1RA,binding
R14141 T20593 T20601 themeOf NFIL-6,binding
R14142 T20593 T20601 themeOf NFIL-6,binding
R14143 T20594 T20601 themeOf NFATP2,binding
R14144 T20594 T20601 themeOf NFATP2,binding
R14145 T20595 T20601 themeOf 3,binding
R14146 T20596 T20601 themeOf IL-1RA,binding
R14147 T20596 T20601 themeOf IL-1RA,binding
R14148 T20596 T20601 themeOf IL-1RA,binding

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T20946 5861-5917 Binding denotes binding of transcription factors to the IL-1RA promoter,
T20945 6600-6629 Transcription denotes messenger RNA was transcribed
T20944 6600-6629 Transcription denotes messenger RNA was transcribed
T20943 6537-6546 Localization denotes PBMC RNA
T20942 5796-5831 Negative_regulation denotes Inhibition of transcription factors
T20941 6963-6977 Protein denotes IL-1RA protein
T20940 5956-5983 Entity denotes of the following substances
T20939 5918-5922 Entity denotes PBMC
T20938 6503-6508 Entity denotes sites
T20937 6454-6457 Protein denotes GGC
T20936 6938-6942 Entity denotes 18 h
T20935 6994-7048 Entity denotes the supernatant by ELISA after 24 h incubation with GP
T20934 5869-5893 Protein denotes of transcription factors
T20933 5807-5831 Protein denotes of transcription factors
T20932 6454-6481 Protein denotes GGC GCA GAA AAG GTA AAA TAT
T20931 6537-6545 Protein denotes PBMC RNA
T20930 6713-6718 Protein denotes GAPDH
T20929 6702-6753 Protein denotes IL-1RA and GAPDH transcripts by PCR with a HotStart
T20928 6415-6422 Protein denotes ATT GCG
T20927 6205-6254 Entity denotes 10 μg/ml cycloheximide (Santa Cruz Biotechnology)
T20926 6451-6452 Protein denotes 3
T20925 6537-6541 Entity denotes PBMC
T20924 6338-6340 Protein denotes GP
T20923 6391-6397 Protein denotes NFIL-6
T20922 6454-6481 Protein denotes GGC GCA GAA AAG GTA AAA TAT
T20921 5901-5907 Protein denotes IL-1RA
T20920 6454-6481 Entity denotes GGC GCA GAA AAG GTA AAA TAT
T20919 6444-6452 Protein denotes NFATP2/3
T20918 7025-7029 Entity denotes 24 h
T20917 5897-5917 Entity denotes the IL-1RA promoter,
T20916 6745-6748 Protein denotes Hot
T20915 6427-6438 Protein denotes CTT AGT GGG
T20914 5942-5983 Entity denotes 1 h with each of the following substances
T20913 6600-6613 Protein denotes messenger RNA
T20912 6921-6926 Protein denotes GAPDH
T20911 6133-6183 Entity denotes ng/ml cyclosporin A (Calbiochem-Merck Biosciences,
T20910 6427-6438 Entity denotes CTT AGT GGG
T20909 6516-6535 Entity denotes the IL-1RA promoter
T20908 6702-6708 Protein denotes IL-1RA
T20907 6959-6961 Protein denotes GP
T20906 6318-6340 Entity denotes 1 h incubation with GP
T20905 6444-6452 Protein denotes NFATP2/3
T20904 6458-6461 Protein denotes GCA
T20903 6280-6282 Protein denotes GP
T20902 6466-6469 Entity denotes AAG
T20901 5951-5983 Entity denotes each of the following substances
T20900 6734-6737 Protein denotes PCR
T20899 6284-6288 Entity denotes Band
T20898 6520-6526 Protein denotes IL-1RA
T20897 6963-6969 Protein denotes IL-1RA
T20896 6842-6848 Protein denotes IL-1RA
T20895 6486-6489 Entity denotes CTA
T20894 6762-6765 Protein denotes PCR
T21360 7453-7459 Protein denotes TSST-1
T21359 7738-7744 Protein denotes TSST-1
T21358 7130-7146 Protein denotes cytokine amounts
T21357 7751-7757 Protein denotes TSST-1
T21356 7466-7472 Protein denotes TSST-1
T21355 7173-7194 Protein denotes transcription factors
T21354 7641-7658 Entity denotes example GP vs LPS
T21353 7453-7477 Protein denotes TSST-1 vs GP/TSST-1 calc
T21352 7641-7658 Entity denotes example GP vs LPS
T21351 7785-7800 Entity denotes the Student's t
T21350 7738-7762 Protein denotes TSST-1 vs GP/TSST-1 calc
T20205 5716-5762 Regulation denotes donors showing no spontaneous cytokine release
T20204 5679-5793 Positive_regulation denotes Only stimulation of PBMC observed in donors showing no spontaneous cytokine release was included in the statistics
T20203 5370-5377 Protein denotes of IL-1
T20202 5696-5703 Entity denotes of PBMC
T20201 5326-5333 Protein denotes of IL-2
T20200 5086-5090 Protein denotes TNFα
T20199 4922-4951 Entity denotes Supernatants of cell cultures
T20198 5746-5754 Protein denotes cytokine
T20197 5101-5105 Protein denotes IL-6
T20196 5489-5502 Protein denotes All cytokines
T20195 5613-5629 Protein denotes Cytokine amounts
T20194 5092-5096 Protein denotes IL-1
T20193 5080-5084 Protein denotes IFNγ
T20192 4900-4921 Protein denotes Cytokine measurements
T20191 5176-5182 Protein denotes IL-1RA
T20190 5259-5265 Protein denotes TGFβ 1
T20189 5187-5191 Protein denotes IL-8
T19688 4140-4147 Entity denotes sc-109,
T19687 3998-4011 Protein denotes c-Rel (61 kD)
T19686 4385-4400 Entity denotes AP-conjugate sc
T19685 4047-4061 Entity denotes PMA-stimulated
T19684 3870-3880 Entity denotes antibodies
T19683 3667-3691 Protein denotes the DNA binding proteins
T19682 4243-4256 Protein denotes NFATc2 (M-20)
T19681 4122-4139 Protein denotes anti-NFκB p65 (A)
T19680 4217-4229 Protein denotes c-rel (N466)
T19679 4780-4790 Protein denotes PBS/1% BSA
T19678 4294-4327 Protein denotes two goat polyclonal IgG1 antisera
T19677 4094-4113 Protein denotes anti-NFκB p50 (NLS)
T19676 4224-4228 Entity denotes N466
T19675 4454-4474 Protein denotes The nuclear proteins
T19674 4671-4682 Protein denotes PBS/1% BSA,
T19673 3741-3744 Protein denotes SRC
T19672 4023-4045 Entity denotes Jurkat nuclear extract
T19671 4212-4232 Entity denotes anti-c-rel (N466) sc
T19670 3708-3712 Protein denotes NFκB
T19669 4329-4362 Entity denotes secondary antibodies (0.8 μg/dot)
T19668 4260-4277 Protein denotes 1151, anti-NFATc1
T19667 4279-4283 Protein denotes K-18
T19666 3739-3760 Protein denotes a SRC96D SNS minifold
T19665 3717-3722 Protein denotes NFAT,
T19664 4642-4652 Entity denotes antibodies
T19663 4063-4092 Entity denotes primary antibodies (1 μg/dot)
T19662 4233-4259 Entity denotes 272, anti-NFATc2 (M-20) sc
T19661 4176-4210 Protein denotes a rabbit polyclonal IgG1 antiserum
T19660 4550-4560 Protein denotes PBS/3% BSA
T19659 4385-4400 Entity denotes AP-conjugate sc
T19658 4140-4170 Entity denotes sc-109, anti-NFκB p52 (447) sc
T19657 3975-3986 Protein denotes p52 (80 kD)
T19656 4715-4769 Entity denotes the alkaline phosphatase-conjugated secondary antibody
T19655 4158-4167 Protein denotes p52 (447)
T19654 3970-3989 Protein denotes NFκB p52 (80 kD) sc
T19160 3219-3259 Binding denotes non-specific binding to nuclear proteins
T19159 3134-3163 Binding denotes the cytokine promoter binding
T19158 3427-3433 Protein denotes p65/50
T19157 3534-3546 Protein denotes of Oct-1 DNA
T19156 3406-3410 Protein denotes NFκB
T19155 3240-3259 Protein denotes to nuclear proteins
T19154 3331-3354 Entity denotes to the nuclear extracts
T19153 3427-3433 Protein denotes p65/50
T19152 3147-3155 Entity denotes promoter
T19151 3134-3163 Protein denotes the cytokine promoter binding
T19150 2767-2783 Entity denotes nuclear extracts
T19149 3548-3571 Entity denotes unrelated DNA, Table 1)
T19148 3384-3401 Entity denotes supershift assays
T19147 3219-3259 Entity denotes non-specific binding to nuclear proteins
T19146 3431-3433 Protein denotes 50
T19145 3434-3442 Entity denotes antibody
T19144 3534-3546 Entity denotes of Oct-1 DNA
T19143 2833-2867 Entity denotes Nuclear extracts of purified PBMCs
T19142 2853-2867 Entity denotes purified PBMCs
T19141 3164-3181 Entity denotes sites (see above)
T18755 2515-2595 Regulation denotes which seem to be crucial for the binding of NFAT and NFκB proteins, respectively
T18754 2540-2581 Binding denotes for the binding of NFAT and NFκB proteins
T18753 1899-1903 Protein denotes TNFα
T18752 2568-2572 Protein denotes NFκB
T18751 2662-2673 Entity denotes Probe Quant
T18750 2441-2466 Entity denotes of the TNFα promoter [55]
T18749 2386-2408 Entity denotes the IFNγ promoter [29]
T18748 1920-1924 Protein denotes IL-6
T18747 2144-2158 Entity denotes Polynucleotide
T18746 1778-1803 Entity denotes Oligonucleotides (Oligos)
T18745 2448-2452 Protein denotes TNFα
T18744 1911-1915 Protein denotes IFNγ
T18743 2556-2581 Protein denotes of NFAT and NFκB proteins
T18742 2171-2174 Protein denotes PNK
T18741 2609-2622 Entity denotes the ds oligos
T18740 2390-2394 Protein denotes IFNγ
T18739 2159-2165 Protein denotes Kinase
T18738 2371-2421 Entity denotes the P2 site of the IFNγ promoter [29] and in the κ
T18737 1895-1934 Entity denotes the TNFα, IL-8, IFNγ and IL-6 promoters
T18736 1905-1909 Protein denotes IL-8
T18735 2171-2178 Protein denotes PNK Kit
T18734 2641-2644 Entity denotes gel
T18733 1860-1862 Entity denotes ds
T18732 1864-1889 Entity denotes oligonucleotides (oligos)
T18731 2559-2563 Protein denotes NFAT
T18730 2128-2169 Protein denotes the Ready-To-Go-Polynucleotide-Kinase Kit
T18228 1557-1617 Entity denotes wild-type LPS from Escherichia coli serotype 0111:B4 (Sigma,
T18227 1765-1767 Protein denotes GP
T18226 1415-1424 Entity denotes h (ELISA)
T18225 1386-1396 Entity denotes 1 h (EMSA)
T18224 1230-1239 Protein denotes cytokines
T18223 1283-1303 Protein denotes sterile pyrogen-free
T18222 1485-1512 Protein denotes TSST-1 (Toxin Technologies,
T18221 1241-1245 Entity denotes PBMC
T18220 1733-1739 Protein denotes TSST-1
T18219 1666-1670 Entity denotes PBMC
T18218 1611-1616 Protein denotes Sigma
T18217 1493-1498 Entity denotes Toxin
T18216 1459-1461 Protein denotes GP
T18215 1772-1775 Entity denotes LPS
T18214 1551-1556 Entity denotes cells
T18213 1468-1521 Entity denotes 250 ng/106 cells TSST-1 (Toxin Technologies, Sarasota
T18212 1726-1728 Protein denotes GP
T17930 1062-1075 Entity denotes 1% penicillin
T17929 1125-1161 Entity denotes 1% 200 mM L-glutamine (BioWhittaker)
T17928 431-444 Entity denotes of human PBMC
T17927 737-741 Entity denotes PBMC
T17926 845-848 Entity denotes gel
T17925 1090-1120 Entity denotes 1% streptomycin (10000 μg/ml),
T17924 1020-1027 Entity denotes low-LPS
T17923 954-962 Entity denotes LPS-free
T17615 64-66 Protein denotes GP
T17614 19-83 Entity denotes Water soluble phosphorylated β-1→3-D-glucan (GP) was prepared as
T17613 369-378 Entity denotes endotoxin
T17612 271-280 Entity denotes endotoxin
R12857 T18743 T18754 themeOf of NFAT and NFκB proteins,for the binding of NFAT and NFκB proteins
R12858 T18749 T18740 partOf the IFNγ promoter [29],IFNγ
R12859 T18750 T18745 partOf of the TNFα promoter [55],TNFα
R12860 T18754 T18755 themeOf for the binding of NFAT and NFκB proteins,"which seem to be crucial for the binding of NFAT and NFκB proteins, respectively"
R13180 T19147 T19160 themeOf non-specific binding to nuclear proteins,non-specific binding to nuclear proteins
R13181 T19152 T19159 themeOf promoter,the cytokine promoter binding
R13182 T19155 T19160 themeOf to nuclear proteins,non-specific binding to nuclear proteins
R13880 T20198 T20205 themeOf cytokine,donors showing no spontaneous cytokine release
R14420 T20909 T20898 partOf the IL-1RA promoter,IL-1RA
R14421 T20913 T20944 themeOf messenger RNA,messenger RNA was transcribed
R14422 T20913 T20945 themeOf messenger RNA,messenger RNA was transcribed
R14423 T20917 T20921 partOf "the IL-1RA promoter,",IL-1RA
R14424 T20925 T20943 locationOf PBMC,PBMC RNA
R14425 T20931 T20943 themeOf PBMC RNA,PBMC RNA
R14426 T20933 T20942 themeOf of transcription factors,Inhibition of transcription factors
R14427 T20934 T20946 themeOf of transcription factors,"binding of transcription factors to the IL-1RA promoter,"

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T20878 7048-7049 . denotes .
T20877 7046-7048 NNP denotes GP
T20876 7041-7045 IN denotes with
T20875 7030-7040 NN denotes incubation
T20874 7028-7029 NN denotes h
T20873 7025-7027 CD denotes 24
T20872 7019-7024 IN denotes after
T20871 7013-7018 NNP denotes ELISA
T20870 7010-7012 IN denotes by
T20869 6998-7009 JJ denotes supernatant
T20868 6994-6997 DT denotes the
T20867 6991-6993 IN denotes in
T20866 6982-6990 VBN denotes measured
T20865 6978-6981 VBD denotes was
T20864 6970-6977 NN denotes protein
T20863 6963-6969 NN denotes IL-1RA
T20862 6961-6962 . denotes .
T20861 6959-6961 NNP denotes GP
T20860 6954-6958 IN denotes with
T20859 6943-6953 NN denotes incubation
T20858 6941-6942 NN denotes h
T20857 6938-6940 CD denotes 18
T20856 6928-6937 VBG denotes following
T20855 6926-6927 -RRB- denotes )
T20854 6921-6926 NNP denotes GAPDH
T20853 6917-6920 IN denotes for
T20852 6910-6916 NNS denotes cycles
T20851 6907-6909 CD denotes 25
T20850 6905-6906 , denotes ,
T20849 6902-6905 NN denotes sec
T20848 6899-6901 CD denotes 45
T20847 6895-6898 IN denotes for
T20846 6893-6894 NNP denotes C
T20845 6892-6893 NN denotes °
T20844 6890-6892 CD denotes 72
T20843 6886-6889 CC denotes and
T20842 6882-6885 NN denotes sec
T20841 6879-6881 CD denotes 30
T20840 6875-6878 IN denotes for
T20839 6873-6874 NNP denotes C
T20838 6872-6873 NN denotes °
T20837 6870-6872 CD denotes 50
T20836 6868-6869 , denotes ,
T20835 6865-6868 NN denotes sec
T20834 6862-6864 CD denotes 30
T20833 6858-6861 IN denotes for
T20832 6856-6857 NNP denotes C
T20831 6855-6856 NN denotes °
T20830 6853-6855 CD denotes 94
T20829 6849-6852 CC denotes and
T20828 6842-6848 NN denotes IL-1RA
T20827 6838-6841 IN denotes for
T20826 6831-6837 NNS denotes cycles
T20825 6828-6830 CD denotes 25
T20824 6826-6827 , denotes ,
T20823 6823-6826 NN denotes sec
T20822 6820-6822 CD denotes 30
T20821 6818-6819 , denotes ,
T20820 6817-6818 NNP denotes C
T20819 6816-6817 NN denotes °
T20818 6814-6816 CD denotes 72
T20817 6812-6813 , denotes ,
T20816 6809-6812 NN denotes sec
T20815 6806-6808 CD denotes 30
T20814 6804-6805 , denotes ,
T20813 6803-6804 NNP denotes C
T20812 6802-6803 NN denotes °
T20811 6800-6802 CD denotes 55
T20810 6798-6799 , denotes ,
T20642 5985-5999 NNS denotes concentrations
T20641 5984-5985 -LRB- denotes (
T20640 5973-5983 NNS denotes substances
T20639 5963-5972 VBG denotes following
T20638 5959-5962 DT denotes the
T20637 5956-5958 IN denotes of
T20636 5951-5955 DT denotes each
T20635 5946-5950 IN denotes with
T20634 5944-5945 NN denotes h
T20633 5942-5943 CD denotes 1
T20632 5938-5941 IN denotes for
T20631 5928-5937 VBN denotes incubated
T20630 5923-5927 VBD denotes were
T20629 5918-5922 NNP denotes PBMC
T20628 5916-5917 , denotes ,
T20627 5908-5916 NN denotes promoter
T20626 5901-5907 NN denotes IL-1RA
T20625 5897-5900 DT denotes the
T20624 5894-5896 TO denotes to
T20623 5886-5893 NNS denotes factors
T20622 5872-5885 NN denotes transcription
T20621 5869-5871 IN denotes of
T20620 5861-5868 JJ denotes binding
T20619 5857-5860 CC denotes and
T20618 5843-5856 NN denotes translocation
T20617 5835-5842 VB denotes inhibit
T20616 5832-5834 TO denotes To
T20615 5824-5831 NNS denotes factors
T20614 5810-5823 NN denotes transcription
T20613 5807-5809 IN denotes of
T20612 5796-5806 NNP denotes Inhibition
T21348 7947-7948 . denotes .
T21347 7946-7947 -RRB- denotes )
T21346 7939-7946 NNP denotes Germany
T21345 7937-7938 , denotes ,
T21344 7930-7937 NNP denotes Erkrath
T21343 7928-7929 , denotes ,
T21342 7920-7928 NNP denotes Software
T21341 7912-7919 NNP denotes Science
T21340 7907-7911 NNP denotes SPSS
T21339 7905-7906 : denotes ;
T21338 7898-7905 NNS denotes Windows
T21337 7894-7897 IN denotes for
T21336 7889-7893 NNS denotes SPSS
T21335 7888-7889 -LRB- denotes (
T21334 7886-7887 , denotes ,
T21333 7885-7886 -RRB- denotes )
T21332 7873-7885 NN denotes distribution
T21331 7862-7872 JJ denotes non-normal
T21330 7861-7862 -LRB- denotes (
T21329 7856-7860 NN denotes test
T21328 7844-7855 NNP denotes Signed-Rank
T21327 7835-7843 NNP denotes Wilcoxon
T21326 7831-7834 DT denotes the
T21325 7828-7830 CC denotes or
T21324 7826-7827 -RRB- denotes )
T21323 7814-7826 NN denotes distribution
T21322 7807-7813 JJ denotes normal
T21321 7806-7807 -LRB- denotes (
T21320 7799-7805 JJS denotes t-test
T21319 7796-7798 POS denotes 's
T21318 7789-7796 NN denotes Student
T21317 7785-7788 DT denotes the
T21316 7779-7784 VBG denotes using
T21315 7770-7778 VBN denotes analysed
T21314 7765-7769 VBD denotes were
T21313 7763-7764 -RRB- denotes )
T21312 7762-7763 . denotes .
T21311 7758-7762 NN denotes calc
T21310 7755-7757 CD denotes -1
T21309 7748-7755 NNP denotes GP/TSST
T21308 7745-7747 VBZ denotes vs
T21307 7738-7744 NNP denotes TSST-1
T21306 7736-7737 NNP denotes +
T21305 7733-7735 NNP denotes GP
T21304 7725-7732 NN denotes example
T21303 7721-7724 IN denotes for
T21302 7720-7721 -LRB- denotes (
T21301 7713-7719 NNS denotes values
T21300 7701-7712 JJ denotes theoretical
T21299 7697-7700 CC denotes and
T21298 7692-7696 NNS denotes data
T21297 7679-7691 JJ denotes experimental
T21296 7671-7678 IN denotes between
T21295 7668-7670 IN denotes as
T21294 7663-7667 RB denotes well
T21293 7660-7662 RB denotes as
T21292 7658-7659 -RRB- denotes )
T21291 7655-7658 NNP denotes LPS
T21290 7652-7654 VBZ denotes vs
T21289 7649-7651 NNP denotes GP
T21288 7641-7648 NN denotes example
T21287 7637-7640 IN denotes for
T21286 7636-7637 -LRB- denotes (
T21285 7631-7635 NNS denotes data
T21284 7618-7630 JJ denotes experimental
T21283 7610-7617 IN denotes between
T21282 7598-7609 NNS denotes differences
T21281 7595-7597 IN denotes of
T21280 7581-7594 NNS denotes Significances
T21279 7579-7580 . denotes .
T21278 7578-7579 CD denotes 6
T21277 7574-7577 CC denotes and
T21276 7572-7573 , denotes ,
T21275 7571-7572 CD denotes 5
T21274 7569-7570 , denotes ,
T21273 7568-7569 CD denotes 4
T21272 7566-7567 , denotes ,
T21271 7565-7566 CD denotes 3
T21270 7563-7564 , denotes ,
T21269 7561-7563 NNP denotes 1B
T21268 7556-7560 NNP denotes Fig.
T21267 7551-7555 IN denotes into
T21266 7544-7550 NNS denotes values
T21265 7538-7543 DT denotes these
T21264 7532-7537 VBD denotes added
T21263 7528-7531 CC denotes and
T21262 7520-7527 NNS denotes effects
T21261 7509-7519 JJ denotes individual
T21260 7505-7508 DT denotes the
T21259 7502-7504 IN denotes of
T21258 7498-7501 NN denotes sum
T21257 7494-7497 DT denotes the
T21256 7483-7493 VBD denotes calculated
T21255 7480-7482 PRP denotes we
T21254 7478-7479 -RRB- denotes )
T21253 7477-7478 . denotes .
T21252 7473-7477 NN denotes calc
T21251 7470-7472 CD denotes -1
T21250 7463-7470 NNP denotes GP/TSST
T21249 7460-7462 VBZ denotes vs
T21248 7453-7459 NNP denotes TSST-1
T21247 7451-7452 NNP denotes +
T21246 7448-7450 NNP denotes GP
T21245 7440-7447 NN denotes example
T21244 7436-7439 IN denotes for
T21243 7435-7436 -LRB- denotes (
T21242 7429-7434 NN denotes value
T21241 7417-7428 JJ denotes theoretical
T21240 7415-7416 DT denotes a
T21239 7412-7414 TO denotes to
T21238 7407-7411 NNS denotes data
T21237 7394-7406 JJ denotes experimental
T21236 7386-7393 VB denotes compare
T21235 7383-7385 TO denotes To
T21234 7381-7382 . denotes .
T21233 7380-7381 -RRB- denotes )
T21232 7368-7380 NN denotes distribution
T21231 7357-7367 JJ denotes non-normal
T21230 7356-7357 -LRB- denotes (
T21229 7351-7355 NN denotes test
T21215 7265-7278 NNS denotes significances
T21214 7251-7264 JJ denotes corresponding
T21213 7247-7250 CC denotes and
T21212 7234-7246 NNS denotes coefficients
T21211 7222-7233 NN denotes Correlation
T21210 7220-7221 . denotes .
T21209 7209-7220 VBN denotes distributed
T21208 7200-7208 RB denotes normally
T21207 7195-7199 VBD denotes were
T21206 7187-7194 NNS denotes factors
T21205 7173-7186 NN denotes transcription
T21204 7170-7172 TO denotes to
T21203 7159-7169 NNS denotes activities
T21202 7151-7158 JJ denotes binding
T21201 7147-7150 CC denotes and
T21200 7139-7146 NNS denotes amounts
T21199 7130-7138 NN denotes cytokine
T21198 7122-7129 IN denotes whether
T21197 7120-7121 , denotes ,
T21196 7112-7120 VB denotes evaluate
T21195 7109-7111 TO denotes to
T21194 7104-7108 VBN denotes used
T21193 7100-7103 VBD denotes was
T21192 7095-7099 NN denotes test
T21191 7076-7094 NNP denotes Kolmogorow-Smirnov
T21190 7072-7075 DT denotes The
T21189 7063-7071 NN denotes analysis
T21188 7051-7062 NNP denotes Statistical
T21228 7342-7350 NNP denotes Spearman
T21227 7338-7341 DT denotes the
T21226 7335-7337 CC denotes or
T21225 7333-7334 -RRB- denotes )
T21224 7321-7333 NN denotes distribution
T21223 7314-7320 JJ denotes normal
T21222 7313-7314 -LRB- denotes (
T21221 7308-7312 NN denotes test
T21220 7300-7307 NNP denotes Pearson
T21219 7296-7299 DT denotes the
T21218 7293-7295 IN denotes by
T21217 7284-7292 VBN denotes analyzed
T21216 7279-7283 VBD denotes were
T20809 6795-6798 NN denotes sec
T20808 6792-6794 CD denotes 30
T20807 6790-6791 , denotes ,
T20806 6789-6790 NNP denotes C
T20805 6788-6789 NN denotes °
T20804 6786-6788 CD denotes 95
T20803 6785-6786 -LRB- denotes (
T20802 6783-6784 -RRB- denotes )
T20801 6777-6783 NNP denotes Biomol
T20800 6776-6777 -LRB- denotes (
T20799 6766-6775 NN denotes mastermix
T20798 6762-6765 NNP denotes PCR
T20797 6760-6761 '' denotes "
T20796 6755-6760 JJ denotes sweet
T20795 6754-6755 `` denotes "
T20794 6745-6753 NNP denotes HotStart
T20793 6743-6744 DT denotes a
T20792 6738-6742 IN denotes with
T20791 6734-6737 NNP denotes PCR
T20790 6731-6733 IN denotes by
T20789 6719-6730 NNS denotes transcripts
T20788 6713-6718 NNP denotes GAPDH
T20787 6709-6712 CC denotes and
T20786 6702-6708 NNP denotes IL-1RA
T20785 6698-6701 IN denotes for
T20784 6689-6697 VBD denotes analysed
T20783 6685-6688 CC denotes and
T20782 6683-6684 -RRB- denotes )
T20781 6677-6683 NNP denotes Biomol
T20780 6676-6677 -LRB- denotes (
T20779 6672-6675 NN denotes kit
T20778 6665-6671 NNP denotes Strand
T20777 6659-6664 NNP denotes First
T20776 6653-6658 NNP denotes Ready
T20775 6645-6652 NNP denotes Reactin
T20774 6640-6644 IN denotes with
T20773 6635-6639 NNP denotes cDNA
T20772 6630-6634 IN denotes into
T20771 6618-6629 VBN denotes transcribed
T20770 6614-6617 VBD denotes was
T20769 6610-6613 NNP denotes RNA
T20768 6600-6609 NN denotes messenger
T20767 6596-6599 CC denotes and
T20766 6594-6595 -RRB- denotes )
T20765 6587-6594 NNP denotes Germany
T20764 6585-6586 , denotes ,
T20763 6579-6585 NNP denotes Qiagen
T20762 6578-6579 -LRB- denotes (
T20761 6574-6577 NN denotes kit
T20760 6567-6573 NNP denotes RNeasy
T20759 6564-6566 DT denotes an
T20758 6559-6563 IN denotes with
T20757 6550-6558 VBN denotes isolated
T20756 6546-6549 VBD denotes was
T20755 6542-6545 NNP denotes RNA
T20754 6537-6541 NNP denotes PBMC
T20753 6535-6536 . denotes .
T20752 6527-6535 NN denotes promoter
T20751 6520-6526 NN denotes IL-1RA
T20750 6516-6519 DT denotes the
T20749 6509-6515 IN denotes within
T20748 6503-6508 NNS denotes sites
T20747 6495-6502 JJ denotes binding
T20746 6493-6494 -RRB- denotes )
T20745 6490-6493 NNP denotes TCT
T20744 6486-6489 NNP denotes CTA
T20701 6282-6283 . denotes .
T20700 6280-6282 NNP denotes GP
T20699 6275-6279 IN denotes with
T20698 6264-6274 NN denotes incubation
T20697 6261-6263 TO denotes to
T20696 6255-6260 RB denotes prior
T20695 6253-6254 -RRB- denotes )
T20694 6240-6253 NNP denotes Biotechnology
T20693 6235-6239 NNP denotes Cruz
T20692 6229-6234 NNP denotes Santa
T20691 6228-6229 -LRB- denotes (
T20690 6214-6227 NN denotes cycloheximide
T20689 6211-6213 NN denotes ml
T20688 6210-6211 NN denotes /
T20687 6208-6210 NN denotes μg
T20686 6205-6207 CD denotes 10
T20685 6203-6204 : denotes ;
T20684 6202-6203 -RRB- denotes )
T20683 6195-6202 NNP denotes Germany
T20682 6193-6194 , denotes ,
T20681 6188-6193 NNP denotes Soden
T20680 6184-6187 NNP denotes Bad
T20679 6182-6183 , denotes ,
T20678 6171-6182 NNP denotes Biosciences
T20677 6154-6170 NNP denotes Calbiochem-Merck
T20676 6153-6154 -LRB- denotes (
T20675 6151-6152 DT denotes A
T20674 6139-6150 NN denotes cyclosporin
T20673 6133-6138 JJ denotes ng/ml
T20672 6129-6132 CD denotes 400
T20671 6127-6128 : denotes ;
T20670 6126-6127 -RRB- denotes )
T20669 6119-6126 NNP denotes Germany
T20668 6117-6118 , denotes ,
T20667 6110-6117 NNP denotes Hamburg
T20666 6108-6109 , denotes ,
T20665 6102-6108 NNP denotes Biomol
T20664 6101-6102 -LRB- denotes (
T20663 6099-6100 , denotes ,
T20662 6098-6099 -RRB- denotes )
T20661 6063-6098 NN denotes dihydroxycinnamic-acid-phenyl-ester
T20660 6062-6063 : denotes -
T20659 6060-6062 SYM denotes ,4
T20658 6051-6060 JJ denotes caffeic-3
T20657 6050-6051 -LRB- denotes (
T20656 6045-6049 NNP denotes CAPE
T20655 6042-6044 IN denotes of
T20654 6039-6041 NN denotes ml
T20653 6038-6039 NN denotes /
T20652 6036-6038 NN denotes μg
T20651 6033-6035 CD denotes 50
T20650 6031-6032 : denotes :
T20649 6030-6031 -RRB- denotes )
T20648 6020-6030 NN denotes inhibition
T20647 6018-6019 NN denotes %
T20646 6016-6018 CD denotes 50
T20645 6011-6015 IN denotes upon
T20644 6005-6010 VBN denotes based
T20643 6000-6004 VBD denotes were
T20180 5793-5794 . denotes .
T20179 5783-5793 NNS denotes statistics
T20178 5779-5782 DT denotes the
T20177 5776-5778 IN denotes in
T20176 5767-5775 VBN denotes included
T20175 5763-5766 VBD denotes was
T20174 5755-5762 NN denotes release
T20173 5746-5754 NN denotes cytokine
T20172 5734-5745 JJ denotes spontaneous
T20171 5731-5733 DT denotes no
T20170 5723-5730 VBG denotes showing
T20169 5716-5722 NNS denotes donors
T20156 5646-5649 DT denotes the
T20155 5639-5645 IN denotes within
T20111 5412-5414 CD denotes 46
T20110 5411-5412 NNP denotes [
T20109 5401-5410 VBN denotes described
T20108 5390-5400 RB denotes previously
T20107 5387-5389 IN denotes as
T20106 5382-5386 VBN denotes done
T20105 5378-5381 VBD denotes was
T20104 5373-5377 NN denotes IL-1
T20103 5370-5372 IN denotes of
T20102 5358-5369 NN denotes bioactivity
T20101 5354-5357 DT denotes the
T20100 5350-5353 IN denotes for
T20099 5340-5349 NN denotes indicator
T20098 5337-5339 DT denotes an
T20097 5334-5336 IN denotes as
T20096 5329-5333 NN denotes IL-2
T20095 5326-5328 IN denotes of
T20094 5311-5325 NN denotes Quantification
T20093 5309-5310 . denotes .
T20092 5308-5309 NNP denotes ]
T20091 5306-5308 CD denotes 57
T20090 5305-5306 NNP denotes [
T20089 5295-5304 VBN denotes described
T20088 5284-5294 RB denotes previously
T20087 5281-5283 IN denotes as
T20086 5275-5280 NNP denotes ELISA
T20085 5272-5274 DT denotes an
T20058 5142-5148 NNP denotes Bender
T20057 5137-5141 IN denotes from
T20056 5131-5136 NNP denotes ELISA
T20055 5128-5130 DT denotes an
T20054 5122-5127 VBG denotes using
T20053 5111-5121 VBN denotes determined
T20052 5106-5110 VBD denotes were
T20051 5101-5105 NNP denotes IL-6
T20050 5097-5100 CC denotes and
T20049 5092-5096 NNP denotes IL-1
T20048 5090-5091 , denotes ,
T20047 5086-5090 NNP denotes TNFα
T20046 5084-5085 , denotes ,
T20045 5080-5084 NNP denotes IFNγ
T20044 5078-5079 . denotes .
T20043 5074-5078 RB denotes once
T20042 5069-5073 RB denotes only
T20041 5062-5068 VBN denotes thawed
T20040 5058-5061 CC denotes and
T20039 5053-5057 VBN denotes used
T20038 5047-5052 IN denotes until
T20037 5045-5046 NNP denotes C
T20036 5044-5045 CD denotes °
T20035 5040-5043 CD denotes -80
T20034 5037-5039 IN denotes at
T20033 5030-5036 VBD denotes stored
T20032 5026-5029 CC denotes and
T20031 5014-5025 NN denotes stimulation
T20030 5008-5013 IN denotes after
T20029 5002-5007 NNS denotes hours
T20028 4999-5001 CD denotes 48
T20027 4995-4998 CC denotes and
T20026 4992-4994 CD denotes 36
T20025 4990-4991 , denotes ,
T20024 4988-4990 CD denotes 30
T20023 4986-4987 , denotes ,
T20022 4984-4986 CD denotes 24
T20021 4982-4983 , denotes ,
T20020 4980-4982 CD denotes 18
T20019 4978-4979 , denotes ,
T20018 4976-4978 CD denotes 12
T20017 4974-4975 , denotes ,
T20016 4973-4974 CD denotes 9
T20015 4971-4972 , denotes ,
T20014 4970-4971 CD denotes 6
T20013 4968-4969 , denotes ,
T20012 4967-4968 CD denotes 3
T20011 4957-4966 VBN denotes harvested
T20010 4952-4956 VBD denotes were
T20009 4943-4951 NNS denotes cultures
T20008 4938-4942 NN denotes cell
T20007 4935-4937 IN denotes of
T20006 4922-4934 NNPS denotes Supernatants
T20005 4909-4921 NNS denotes measurements
T20004 4900-4908 NNP denotes Cytokine
T19639 4897-4898 . denotes .
T19638 4872-4876 NNP denotes Inc.
T19637 4859-4871 NNPS denotes Laboratories
T19636 4852-4858 NNP denotes Vector
T19635 4851-4852 -LRB- denotes (
T20154 5635-5638 DT denotes all
T20153 5630-5634 VBD denotes were
T20152 5622-5629 NNS denotes amounts
T20151 5613-5621 NNP denotes Cytokine
T20150 5611-5612 . denotes .
T20149 5610-5611 -RRB- denotes )
T20148 5604-5610 NNP denotes BioRad
T20147 5602-5603 , denotes ,
T20146 5596-5602 NNP denotes Reader
T20145 5585-5595 NNP denotes Microplate
T20144 5582-5584 CC denotes or
T20143 5574-5581 NNP denotes Austria
T20142 5572-5573 , denotes ,
T20141 5564-5572 NNP denotes Salzburg
T20140 5562-5563 , denotes ,
T20139 5555-5562 NNP denotes Labotec
T20138 5548-5554 NNP denotes Anthos
T20137 5547-5548 -LRB- denotes (
T20136 5540-5546 NN denotes reader
T20135 5534-5539 NN denotes plate
T20134 5528-5533 NNP denotes ELISA
T20133 5525-5527 DT denotes an
T20132 5519-5524 VBG denotes using
T20131 5508-5518 VBN denotes quantified
T20130 5503-5507 VBD denotes were
T20129 5493-5502 NNS denotes cytokines
T20128 5489-5492 DT denotes All
T20127 5487-5488 . denotes .
T20126 5478-5487 NNS denotes standards
T20125 5474-5477 DT denotes the
T20124 5470-5473 IN denotes for
T20123 5465-5469 VBN denotes used
T20122 5460-5464 DT denotes that
T20121 5457-5459 IN denotes as
T20120 5450-5456 NN denotes buffer
T20119 5445-5449 JJ denotes same
T20118 5441-5444 DT denotes the
T20117 5438-5440 IN denotes in
T20116 5430-5437 VBN denotes diluted
T20115 5425-5429 VBD denotes were
T20114 5417-5424 NNS denotes Samples
T20113 5415-5416 . denotes .
T20112 5414-5415 NNP denotes ]
T19634 4847-4850 NN denotes kit
T19633 4838-4846 VBG denotes staining
T19632 4836-4837 NN denotes ®
T19631 4826-4836 NNP denotes Vectastain
T19630 4822-4825 DT denotes the
T19629 4816-4821 VBG denotes using
T19628 4806-4815 VBN denotes developed
T19627 4802-4805 VBD denotes was
T19626 4797-4801 NN denotes blot
T19625 4793-4796 DT denotes The
T19624 4791-4792 . denotes .
T19623 4790-4791 -RRB- denotes )
T19622 4787-4790 NNP denotes BSA
T19621 4785-4786 NN denotes %
T19620 4780-4785 CD denotes PBS/1
T19619 4777-4779 IN denotes in
T19618 4771-4776 CD denotes 1:500
T19617 4770-4771 -LRB- denotes (
T19616 4761-4769 NN denotes antibody
T19615 4751-4760 JJ denotes secondary
T19614 4728-4750 JJ denotes phosphatase-conjugated
T19613 4719-4727 JJ denotes alkaline
T19612 4715-4718 DT denotes the
T19611 4710-4714 IN denotes with
T19610 4700-4709 VBN denotes incubated
T19609 4696-4699 CC denotes and
T19569 4488-4492 IN denotes onto
T19568 4480-4487 VBN denotes blotted
T19567 4475-4479 VBD denotes were
T19566 4466-4474 NNS denotes proteins
T19565 4458-4465 JJ denotes nuclear
T19564 4454-4457 DT denotes The
T19563 4452-4453 . denotes .
T19562 4445-4452 NN denotes sc-2022
T19561 4432-4444 NNP denotes AP-conjugate
T19560 4428-4431 NNP denotes IgG
T19559 4418-4427 JJ denotes anti-goat
T19558 4411-4417 JJ denotes donkey
T19557 4407-4410 CC denotes and
T19556 4405-4406 , denotes ,
T19555 4398-4405 JJ denotes sc-2007
T19554 4385-4397 NNP denotes AP-conjugate
T19553 4381-4384 NNP denotes IgG
T19552 4369-4380 JJ denotes anti-rabbit
T19551 4364-4368 VB denotes goat
T19550 4362-4363 : denotes :
T19549 4361-4362 -RRB- denotes )
T19548 4358-4361 VBP denotes dot
T19547 4357-4358 NN denotes /
T19546 4355-4357 NN denotes μg
T19545 4351-4354 CD denotes 0.8
T19544 4350-4351 -LRB- denotes (
T19543 4339-4349 NNS denotes antibodies
T19542 4329-4338 JJ denotes secondary
T19541 4327-4328 : denotes ;
T19540 4319-4327 NN denotes antisera
T19539 4314-4318 JJ denotes IgG1
T19538 4303-4313 JJ denotes polyclonal
T19537 4298-4302 NN denotes goat
T19536 4294-4297 CD denotes two
T19535 4292-4293 , denotes ,
T19534 4285-4292 JJ denotes sc-1149
T19533 4283-4284 -RRB- denotes )
T19532 4279-4283 NN denotes K-18
T19531 4278-4279 -LRB- denotes (
T19530 4266-4277 JJ denotes anti-NFATc1
T19529 4264-4265 , denotes ,
T19528 4257-4264 JJ denotes sc-1151
T19527 4255-4256 -RRB- denotes )
T19526 4251-4255 NN denotes M-20
T19525 4250-4251 -LRB- denotes (
T19524 4238-4249 JJ denotes anti-NFATc2
T19523 4236-4237 , denotes ,
T19522 4230-4236 JJ denotes sc-272
T19521 4228-4229 -RRB- denotes )
T19520 4224-4228 NNP denotes N466
T19519 4223-4224 -LRB- denotes (
T19518 4212-4222 JJ denotes anti-c-rel
T19517 4210-4211 , denotes ,
T19516 4201-4210 NN denotes antiserum
T19515 4196-4200 JJ denotes IgG1
T19514 4185-4195 NN denotes polyclonal
T19513 4178-4184 NN denotes rabbit
T19512 4176-4177 DT denotes a
T19511 4174-4175 , denotes ,
T19510 4168-4174 JJ denotes sc-848
T19509 4166-4167 -RRB- denotes )
T19508 4163-4166 CD denotes 447
T19507 4162-4163 -LRB- denotes (
T19506 4158-4161 NN denotes p52
T19505 4148-4157 JJ denotes anti-NFκB
T19504 4146-4147 , denotes ,
T19503 4140-4146 JJ denotes sc-109
T19502 4138-4139 -RRB- denotes )
T19501 4137-4138 NNP denotes A
T19500 4136-4137 -LRB- denotes (
T19499 4132-4135 NNS denotes p65
T19498 4122-4131 JJ denotes anti-NFκB
T19497 4120-4121 , denotes ,
T19496 4114-4120 JJ denotes sc-114
T19495 4112-4113 -RRB- denotes )
T19494 4109-4112 NNP denotes NLS
T19493 4108-4109 -LRB- denotes (
T19492 4104-4107 JJ denotes p50
T19491 4094-4103 JJ denotes anti-NFκB
T19490 4092-4093 : denotes :
T19489 4091-4092 -RRB- denotes )
T19488 4088-4091 VBP denotes dot
T19487 4087-4088 NN denotes /
T19486 4085-4087 NN denotes μg
T19485 4083-4084 CD denotes 1
T19484 4082-4083 -LRB- denotes (
T19483 4071-4081 NNS denotes antibodies
T19482 4063-4070 JJ denotes primary
T19481 4061-4062 : denotes ;
T19480 4047-4061 JJ denotes PMA-stimulated
T19479 4045-4046 , denotes ,
T19478 4038-4045 VB denotes extract
T19477 4030-4037 JJ denotes nuclear
T19476 4023-4029 NNP denotes Jurkat
T19475 4021-4022 : denotes ;
T19474 4012-4021 NN denotes sc-4030WB
T19473 4010-4011 -RRB- denotes )
T19472 4008-4010 NN denotes kD
T19471 4005-4007 CD denotes 61
T19470 4004-4005 -LRB- denotes (
T19469 3998-4003 NNP denotes c-Rel
T19468 3996-3997 , denotes ,
T19467 3987-3996 NN denotes sc-4095WB
T19466 3985-3986 -RRB- denotes )
T19465 3983-3985 NNP denotes kD
T19464 3980-3982 CD denotes 80
T19463 3979-3980 -LRB- denotes (
T19462 3975-3978 CD denotes p52
T19461 3970-3974 NNP denotes NFκB
T19460 3968-3969 : denotes :
T19459 3967-3968 -RRB- denotes )
T19458 3961-3967 NN denotes ng/dot
T19457 3958-3960 CD denotes 10
T19456 3957-3958 -LRB- denotes (
T19455 3948-3956 NNS denotes controls
T19454 3939-3947 JJ denotes positive
T19453 3937-3938 : denotes :
T19452 3936-3937 -RRB- denotes )
T19451 3929-3936 NNP denotes Germany
T19450 3927-3928 , denotes ,
T19449 3917-3927 NNP denotes Heidelberg
T19448 3915-3916 , denotes ,
T19447 3902-3915 NNP denotes Biotechnology
T19446 3897-3901 NNP denotes Cruz
T19445 3891-3896 NNP denotes Santa
T19444 3886-3890 IN denotes from
T19443 3882-3885 DT denotes all
T19442 3881-3882 -LRB- denotes (
T19441 3870-3880 NNS denotes antibodies
T19440 3866-3869 CC denotes and
T19439 3857-3865 NNS denotes controls
T19438 3848-3856 JJ denotes positive
T19437 3838-3847 VBG denotes following
T19436 3834-3837 DT denotes the
T19435 3829-3833 IN denotes with
T19434 3819-3828 VBN denotes performed
T19433 3814-3818 VBD denotes were
T19432 3812-3813 -RRB- denotes )
T19431 3805-3812 NNP denotes Germany
T19430 3803-3804 , denotes ,
T19429 3797-3803 NNP denotes Dassel
T19428 3795-3796 , denotes ,
T19427 3789-3795 NNP denotes Schüll
T19426 3787-3788 CC denotes &
T19425 3776-3786 NNP denotes Schleicher
T19424 3775-3776 -LRB- denotes (
T19423 3767-3774 NN denotes blotter
T19422 3763-3766 VBP denotes dot
T19421 3761-3762 PRP denotes I
T19420 3752-3760 NN denotes minifold
T19419 3748-3751 NNP denotes SNS
T19418 3741-3747 NNP denotes SRC96D
T19417 3739-3740 DT denotes a
T19416 3733-3738 VBG denotes using
T19415 3727-3732 NNS denotes blots
T19414 3723-3726 VBP denotes dot
T19413 3721-3722 , denotes ,
T19412 3717-3721 NNP denotes NFAT
T19411 3713-3716 CC denotes and
T19410 3708-3712 NNP denotes NFκB
T19409 3705-3707 TO denotes to
T19408 3697-3704 VBN denotes related
T19407 3692-3696 VBD denotes were
T19406 3683-3691 NNS denotes proteins
T19405 3675-3682 JJ denotes binding
T19404 3671-3674 NNP denotes DNA
T19403 3667-3670 DT denotes the
T19402 3659-3666 IN denotes whether
T19401 3649-3658 VB denotes determine
T19400 3646-3648 TO denotes To
T19399 3641-3645 NN denotes blot
T19398 3639-3640 -RRB- denotes )
T19397 3636-3639 NNP denotes Dot
T19396 3635-3636 -LRB- denotes (
T19395 3628-3634 NNP denotes Immuno
T19137 3625-3626 . denotes .
T19136 3618-3625 JJ denotes binding
T19135 3609-3617 JJ denotes specific
T19134 3604-3608 IN denotes with
T19133 3596-3603 VB denotes compete
T19132 3592-3595 RB denotes not
T19131 3588-3591 VBD denotes did
T19130 3582-3587 WDT denotes which
T19129 3580-3581 , denotes ,
T19128 3576-3580 VBN denotes used
T19127 3572-3575 VBD denotes was
T19126 3570-3571 -RRB- denotes )
T19125 3569-3570 CD denotes 1
T19124 3563-3568 NNP denotes Table
T19123 3561-3562 , denotes ,
T19122 3558-3561 NN denotes DNA
T19121 3548-3557 JJ denotes unrelated
T19120 3547-3548 -LRB- denotes (
T19119 3543-3546 NNP denotes DNA
T19118 3537-3542 NNP denotes Oct-1
T19117 3534-3536 IN denotes of
T19608 4690-4695 RB denotes again
T19607 4683-4689 VBD denotes washed
T19606 4681-4682 , denotes ,
T19605 4678-4681 NNP denotes BSA
T19604 4676-4677 NN denotes %
T19603 4671-4676 CD denotes PBS/1
T19602 4668-4670 IN denotes in
T19601 4661-4667 CD denotes 1:2000
T19600 4653-4660 VBN denotes diluted
T19599 4642-4652 NNS denotes antibodies
T19598 4634-4641 JJ denotes primary
T19597 4630-4633 DT denotes the
T19596 4625-4629 IN denotes with
T19595 4615-4624 JJ denotes overnight
T19594 4605-4614 VBN denotes incubated
T19593 4601-4604 VBD denotes was
T19592 4596-4600 NN denotes blot
T19591 4592-4595 DT denotes the
T19590 4590-4591 , denotes ,
T19589 4589-4590 -RRB- denotes )
T19588 4582-4589 NNP denotes Germany
T19587 4580-4581 , denotes ,
T19586 4569-4580 NNP denotes Deisenhofen
T19585 4567-4568 , denotes ,
T19584 4562-4567 NNP denotes Fluka
T19583 4561-4562 -LRB- denotes (
T19582 4557-4560 NNP denotes BSA
T19581 4555-4556 NN denotes %
T19580 4550-4555 CD denotes PBS/3
T19579 4545-4549 IN denotes with
T19578 4536-4544 VBG denotes blocking
T19577 4530-4535 IN denotes After
T19576 4528-4529 . denotes .
T19575 4527-4528 -RRB- denotes )
T19574 4520-4527 NNP denotes Bio-Rad
T19573 4519-4520 -LRB- denotes (
T19572 4510-4518 NN denotes membrane
T19571 4495-4509 JJ denotes nitrocellulose
T19570 4493-4494 DT denotes a
T19116 3527-3533 NN denotes excess
T19115 3521-3526 NN denotes molar
T19114 3513-3520 JJ denotes 30-fold
T19113 3511-3512 DT denotes a
T19112 3509-3510 , denotes ,
T19111 3500-3509 NNS denotes reactions
T19110 3487-3499 JJ denotes non-specific
T19109 3479-3486 VB denotes exclude
T19108 3476-3478 TO denotes To
T19107 3474-3475 . denotes .
T19106 3473-3474 -RRB- denotes )
T19105 3468-3473 VBN denotes shown
T19104 3464-3467 RB denotes not
T19103 3459-3463 NNS denotes data
T19102 3458-3459 -LRB- denotes (
T19101 3448-3457 VBN denotes performed
T19100 3443-3447 VBD denotes were
T19099 3434-3442 NN denotes antibody
T19098 3427-3433 CD denotes p65/50
T19097 3418-3426 VBN denotes combined
T19096 3416-3417 DT denotes a
T19095 3411-3415 IN denotes with
T19094 3406-3410 NNP denotes NFκB
T19093 3402-3405 IN denotes for
T19092 3395-3401 NNS denotes assays
T19091 3384-3394 JJ denotes supershift
T19067 3270-3281 NNS denotes experiments
T19066 3265-3269 JJ denotes such
T19065 3261-3264 IN denotes For
T19064 3259-3260 . denotes .
T19063 3251-3259 NNS denotes proteins
T19062 3243-3250 JJ denotes nuclear
T19061 3240-3242 TO denotes to
T19060 3232-3239 JJ denotes binding
T19059 3219-3231 JJ denotes non-specific
T19058 3211-3218 VB denotes prevent
T19057 3208-3210 TO denotes to
T19056 3202-3207 NN denotes order
T19055 3199-3201 IN denotes in
T19054 3195-3198 RP denotes out
T19053 3187-3194 VBN denotes carried
T19052 3182-3186 VBD denotes were
T19051 3180-3181 -RRB- denotes )
T19050 3175-3180 IN denotes above
T19049 3171-3174 VB denotes see
T19048 3170-3171 -LRB- denotes (
T19047 3164-3169 NNS denotes sites
T19046 3156-3163 JJ denotes binding
T19045 3147-3155 NN denotes promoter
T19044 3138-3146 NN denotes cytokine
T19043 3134-3137 DT denotes the
T19042 3131-3133 IN denotes of
T19041 3124-3130 NNS denotes oligos
T19040 3116-3123 VBN denotes mutated
T19039 3106-3115 JJ denotes unlabeled
T19038 3102-3105 CC denotes and
T19037 3094-3101 VBN denotes labeled
T19036 3089-3093 IN denotes with
T19035 3077-3088 NNS denotes experiments
T19034 3065-3076 NN denotes Competition
T19033 3063-3064 . denotes .
T19032 3055-3063 NNS denotes controls
T19031 3042-3054 JJ denotes unstimulated
T19030 3039-3041 IN denotes of
T19029 3033-3038 NNS denotes bands
T19028 3030-3032 TO denotes to
T19027 3019-3029 VBN denotes normalized
T19026 3015-3018 VBD denotes was
T19025 3009-3014 NNS denotes bands
T19024 3001-3008 VBN denotes shifted
T19023 2998-3000 IN denotes of
T19022 2988-2997 NN denotes intensity
T19021 2984-2987 DT denotes The
T19020 2982-2983 . denotes .
T19019 2981-2982 NNP denotes ]
T19018 2979-2981 CD denotes 56
T19017 2978-2979 NNP denotes [
T19016 2972-2977 NN denotes setup
T19015 2959-2971 JJ denotes experimental
T19014 2955-2958 PRP$ denotes our
T19013 2951-2954 IN denotes for
T19012 2942-2950 VBN denotes modified
T19011 2938-2941 CC denotes and
T19010 2936-2937 NNP denotes ]
T19009 2934-2936 CD denotes 27
T19008 2933-2934 NNP denotes [
T19007 2929-2932 FW denotes al.
T19006 2926-2928 FW denotes et
T19005 2920-2925 NNP denotes Trede
T19004 2917-2919 IN denotes by
T19003 2907-2916 VBN denotes described
T19002 2897-2906 NN denotes technique
T19001 2895-2896 DT denotes a
T19000 2892-2894 TO denotes to
T18999 2882-2891 VBG denotes according
T18998 2873-2881 JJ denotes prepared
T18997 2868-2872 VBD denotes were
T18996 2862-2867 NNS denotes PBMCs
T18995 2853-2861 JJ denotes purified
T18994 2850-2852 IN denotes of
T18993 2841-2849 NNS denotes extracts
T18992 2833-2840 NNP denotes Nuclear
T18991 2831-2832 -RRB- denotes )
T18990 2827-2831 NNP denotes EMSA
T18989 2826-2827 -LRB- denotes (
T18988 2819-2825 NNS denotes assays
T18987 2813-2818 NN denotes shift
T18986 2804-2812 NN denotes mobility
T18985 2788-2803 JJ denotes electrophoretic
T18984 2784-2787 CC denotes and
T18983 2775-2783 NNS denotes extracts
T18982 2767-2774 JJ denotes nuclear
T18981 2764-2766 IN denotes of
T18980 2752-2763 NNP denotes Preparation
T18714 2749-2750 . denotes .
T18713 2737-2749 NNS denotes instructions
T18712 2734-2736 POS denotes 's
T18711 2722-2734 NN denotes manufacturer
T18710 2718-2721 DT denotes the
T18709 2715-2717 TO denotes to
T18708 2705-2714 VBG denotes according
T18707 2703-2704 -RRB- denotes )
T18706 2694-2703 NNP denotes Pharmacia
T18705 2693-2694 -LRB- denotes (
T18704 2685-2692 NNP denotes Columns
T18703 2679-2684 NNP denotes Micro
T18702 2674-2678 NNP denotes G-50
T18701 2668-2673 NN denotes Quant
T18700 2662-2667 NNP denotes Probe
T18699 2656-2661 VBG denotes using
T18698 2645-2655 NN denotes filtration
T18697 2641-2644 NN denotes gel
T18696 2637-2640 IN denotes via
T18695 2628-2636 VBN denotes purified
T18694 2623-2627 VBD denotes were
T18693 2616-2622 NNS denotes oligos
T18692 2613-2615 NNS denotes ds
T18691 2609-2612 DT denotes the
T18690 2607-2608 , denotes ,
T18689 2597-2607 RB denotes Afterwards
T18688 2595-2596 . denotes .
T18687 2583-2595 RB denotes respectively
T18686 2581-2582 , denotes ,
T18685 2573-2581 NNS denotes proteins
T18684 2568-2572 NNP denotes NFκB
T18683 2564-2567 CC denotes and
T18682 2559-2563 NNP denotes NFAT
T18681 2556-2558 IN denotes of
T18680 2548-2555 JJ denotes binding
T18679 2544-2547 DT denotes the
T18678 2540-2543 IN denotes for
T18677 2532-2539 JJ denotes crucial
T18676 2529-2531 VB denotes be
T18675 2526-2528 TO denotes to
T18674 2521-2525 VBP denotes seem
T18673 2515-2520 WDT denotes which
T18672 2513-2514 , denotes ,
T18671 2504-2513 NNS denotes sequences
T18670 2499-2503 IN denotes with
T18669 2489-2498 VB denotes interfere
T18668 2486-2488 TO denotes to
T18667 2477-2485 VBN denotes reported
T18666 2472-2476 VBN denotes been
T18665 2467-2471 VBP denotes have
T18664 2465-2466 NNP denotes ]
T18663 2463-2465 CD denotes 55
T18662 2462-2463 NNP denotes [
T18661 2453-2461 NN denotes promoter
T18660 2448-2452 NNP denotes TNFα
T18659 2444-2447 DT denotes the
T18658 2441-2443 IN denotes of
T18657 2432-2440 NN denotes sequence
T18656 2422-2431 NN denotes consensus
T18655 2420-2421 NN denotes κ
T18654 2416-2419 DT denotes the
T18653 2413-2415 IN denotes in
T18652 2409-2412 CC denotes and
T18651 2407-2408 NNP denotes ]
T18650 2405-2407 CD denotes 29
T18649 2404-2405 NNP denotes [
T18648 2395-2403 NN denotes promoter
T18647 2390-2394 NNP denotes IFNγ
T18646 2386-2389 DT denotes the
T18645 2383-2385 IN denotes of
T18644 2378-2382 NN denotes site
T18643 2375-2377 NNP denotes P2
T18642 2371-2374 DT denotes the
T18641 2368-2370 IN denotes in
T18640 2358-2367 NNS denotes Mutations
T18639 2356-2357 . denotes .
T18638 2349-2356 NN denotes control
T18637 2338-2348 JJ denotes additional
T18636 2335-2337 DT denotes an
T18635 2332-2334 IN denotes as
T18634 2327-2331 VBN denotes used
T18633 2322-2326 VBD denotes were
T18632 2315-2321 NNS denotes oligos
T18631 2307-2314 VBN denotes mutated
T18630 2305-2306 , denotes ,
T18629 2299-2305 NNS denotes oligos
T18628 2295-2298 DT denotes the
T18627 2292-2294 IN denotes of
T18626 2280-2291 NN denotes specificity
T18625 2272-2279 JJ denotes binding
T19090 3382-3383 , denotes ,
T19089 3375-3382 JJ denotes binding
T19088 3372-3374 IN denotes of
T19087 3360-3371 NN denotes specificity
T19086 3356-3359 IN denotes For
T19085 3354-3355 . denotes .
T19084 3346-3354 NNS denotes extracts
T19083 3338-3345 JJ denotes nuclear
T19082 3334-3337 DT denotes the
T19081 3331-3333 TO denotes to
T19080 3325-3330 VBN denotes added
T19079 3321-3324 VBD denotes was
T19078 3314-3320 NNS denotes oligos
T19077 3304-3313 JJ denotes unlabeled
T19076 3301-3303 IN denotes of
T19075 3299-3300 -RRB- denotes )
T19074 3298-3299 NN denotes ×
T19073 3296-3298 CD denotes 50
T19072 3294-3295 CD denotes 5
T19071 3293-3294 -LRB- denotes (
T19070 3286-3292 JJ denotes excess
T19069 3283-3285 DT denotes an
T19068 3281-3282 , denotes ,
T18624 2268-2271 DT denotes the
T18623 2263-2267 VB denotes test
T18622 2260-2262 TO denotes To
T18621 2258-2259 . denotes .
T18620 2246-2258 NNS denotes instructions
T18619 2243-2245 POS denotes 's
T18618 2231-2243 NN denotes manufacturer
T18617 2227-2230 DT denotes the
T18616 2224-2226 TO denotes to
T18615 2214-2223 VBG denotes according
T18614 2212-2213 -RRB- denotes )
T18613 2205-2212 NNP denotes Germany
T18612 2203-2204 , denotes ,
T18611 2195-2203 NNP denotes Freiburg
T18610 2193-2194 , denotes ,
T18609 2190-2193 NNP denotes LKB
T18608 2180-2189 NNP denotes Pharmacia
T18607 2178-2179 , denotes ,
T18606 2175-2178 NNP denotes Kit
T18605 2171-2174 NNP denotes PNK
T18604 2170-2171 -LRB- denotes (
T18603 2166-2169 NNP denotes Kit
T18602 2132-2165 NNP denotes Ready-To-Go-Polynucleotide-Kinase
T18601 2128-2131 DT denotes the
T18600 2122-2127 VBG denotes using
T18599 2120-2121 -RRB- denotes )
T18595 2097-2098 , denotes ,
T18594 2089-2097 NNP denotes Amersham
T18593 2087-2088 , denotes ,
T18592 2080-2087 NNP denotes Ci/mmol
T18591 2074-2079 CD denotes 3,000
T18590 2073-2074 -LRB- denotes (
T18589 2065-2072 NNP denotes γ-P-ATP
T18588 2064-2065 '' denotes '
T18587 2063-2064 CD denotes 5
T18586 2058-2062 IN denotes with
T18585 2046-2057 JJ denotes 32P-labeled
T18584 2042-2045 CC denotes and
T18583 2040-2041 -RRB- denotes )
T18582 2033-2040 NNP denotes Germany
T18581 2031-2032 , denotes ,
T18580 2025-2031 NNP denotes Berlin
T18579 2023-2024 , denotes ,
T18578 2016-2023 NNP denotes Molbiol
T18577 2012-2015 NNP denotes TIB
T18576 2011-2012 -LRB- denotes (
T18575 2009-2010 , denotes ,
T18574 2008-2009 -RRB- denotes )
T18573 2007-2008 CD denotes 1
T18572 2001-2006 NNP denotes Table
T18571 1998-2000 IN denotes in
T18570 1986-1997 VBN denotes illustrated
T18569 1985-1986 -LRB- denotes (
T18568 1978-1984 NNS denotes oligos
T18567 1976-1977 -RRB- denotes )
T18566 1974-1976 NN denotes ss
T18565 1973-1974 -LRB- denotes (
T18564 1964-1972 VBN denotes stranded
T18563 1957-1963 JJ denotes single
T18562 1952-1956 IN denotes from
T18561 1940-1951 VBN denotes synthesised
T18560 1935-1939 VBD denotes were
T18559 1925-1934 NNS denotes promoters
T18558 1920-1924 NNP denotes IL-6
T18557 1916-1919 CC denotes and
T18556 1911-1915 NNP denotes IFNγ
T18555 1909-1910 , denotes ,
T18554 1905-1909 NNP denotes IL-8
T18553 1903-1904 , denotes ,
T18552 1899-1903 NNP denotes TNFα
T18551 1895-1898 DT denotes the
T18550 1890-1894 IN denotes from
T18549 1888-1889 -RRB- denotes )
T18548 1882-1888 NNS denotes oligos
T18547 1881-1882 -LRB- denotes (
T18546 1864-1880 NNS denotes oligonucleotides
T18545 1862-1863 -RRB- denotes )
T18544 1860-1862 NNS denotes ds
T18543 1859-1860 -LRB- denotes (
T18542 1843-1858 JJ denotes double-stranded
T18541 1829-1842 JJ denotes complementary
T18540 1825-1828 DT denotes The
T18539 1814-1824 NN denotes P-Labeling
T18538 1813-1814 : denotes -
T18537 1810-1813 SYM denotes -32
T18536 1809-1810 '' denotes '
T18535 1808-1809 CD denotes 5
T18534 1804-1807 CC denotes and
T18533 1802-1803 -RRB- denotes )
T18532 1796-1802 NNP denotes Oligos
T18531 1795-1796 -LRB- denotes (
T18530 1778-1794 NNS denotes Oligonucleotides
T18208 1772-1775 NNP denotes LPS
T18207 1768-1771 CC denotes and
T18206 1765-1767 NNP denotes GP
T18205 1762-1764 IN denotes of
T18204 1750-1761 NN denotes combination
T18203 1748-1749 DT denotes a
T18202 1743-1747 IN denotes with
T18201 1740-1742 CC denotes or
T18200 1733-1739 NNP denotes TSST-1
T18199 1729-1732 CC denotes and
T18198 1726-1728 NNP denotes GP
T18197 1723-1725 IN denotes of
T18196 1711-1722 NN denotes combination
T18195 1709-1710 DT denotes a
T18194 1704-1708 IN denotes with
T18193 1689-1703 RB denotes simultaneously
T18192 1676-1688 VBN denotes supplemented
T18191 1671-1675 VBD denotes were
T18190 1666-1670 NNP denotes PBMC
T18189 1664-1665 , denotes ,
T18188 1653-1664 NNS denotes experiments
T18187 1639-1652 JJ denotes costimulatory
T18186 1636-1638 IN denotes In
T18185 1634-1635 . denotes .
T18184 1633-1634 -RRB- denotes )
T18183 1626-1633 NNP denotes Germany
T18182 1624-1625 , denotes ,
T18181 1618-1624 NNP denotes Munich
T18180 1616-1617 , denotes ,
T18179 1611-1616 NNP denotes Sigma
T18178 1610-1611 -LRB- denotes (
T18177 1607-1609 NN denotes B4
T18176 1606-1607 : denotes :
T18175 1602-1606 CD denotes 0111
T18174 1593-1601 VBP denotes serotype
T18173 1588-1592 NNS denotes coli
T18172 1576-1587 NNP denotes Escherichia
T18171 1571-1575 IN denotes from
T18170 1567-1570 NNP denotes LPS
T18169 1557-1566 JJ denotes wild-type
T18168 1551-1556 NNS denotes cells
T18167 1544-1550 CD denotes ng/106
T18166 1540-1543 CD denotes 250
T18165 1535-1539 IN denotes with
T18164 1532-1534 CC denotes or
T18163 1530-1531 -RRB- denotes )
T18162 1527-1530 NNP denotes USA
T18161 1525-1526 , denotes ,
T18160 1523-1525 NNP denotes FL
T18159 1521-1522 , denotes ,
T18158 1513-1521 NNP denotes Sarasota
T18157 1511-1512 , denotes ,
T18156 1499-1511 NNPS denotes Technologies
T18155 1493-1498 NNP denotes Toxin
T18154 1492-1493 -LRB- denotes (
T18153 1485-1491 NNP denotes TSST-1
T18152 1479-1484 NNS denotes cells
T18151 1472-1478 CD denotes ng/106
T18150 1468-1471 CD denotes 250
T18149 1463-1467 IN denotes with
T18148 1461-1462 , denotes ,
T18147 1459-1461 NNP denotes GP
T18146 1456-1458 IN denotes of
T18145 1450-1455 NNS denotes cells
T18144 1446-1449 CD denotes 106
T18143 1442-1445 IN denotes per
T18142 1439-1441 NN denotes μg
T18141 1435-1438 CD denotes 100
T18140 1432-1434 CC denotes or
T18139 1430-1431 CD denotes 1
T18138 1425-1429 IN denotes with
T18137 1423-1424 -RRB- denotes )
T18136 1418-1423 NNP denotes ELISA
T18135 1417-1418 -LRB- denotes (
T18134 1415-1416 NN denotes h
T18133 1412-1414 CD denotes 48
T18132 1409-1411 TO denotes to
T18131 1407-1408 NN denotes h
T18130 1405-1406 CD denotes 0
T18129 1400-1404 IN denotes from
T18128 1397-1399 CC denotes or
T18127 1395-1396 -RRB- denotes )
T18126 1391-1395 NNP denotes EMSA
T18125 1390-1391 -LRB- denotes (
T18124 1388-1389 NN denotes h
T18123 1386-1387 CD denotes 1
T18122 1382-1385 IN denotes for
T18121 1371-1381 VBN denotes stimulated
T18120 1366-1370 VBD denotes were
T18119 1357-1365 NNS denotes Cultures
T18118 1355-1356 . denotes .
T18117 1354-1355 -RRB- denotes )
T18116 1347-1354 NNP denotes Germany
T18115 1345-1346 , denotes ,
T18114 1335-1345 NNP denotes Heidelberg
T18113 1333-1334 , denotes ,
T18112 1327-1333 NNP denotes Falcon
T18111 1326-1327 -LRB- denotes (
T18110 1319-1325 NNS denotes plates
T18109 1311-1318 NN denotes culture
T18108 1304-1310 JJ denotes 6-well
T18107 1291-1303 JJ denotes pyrogen-free
T18106 1283-1290 JJ denotes sterile
T18105 1280-1282 IN denotes in
T18104 1277-1279 NN denotes ml
T18103 1275-1276 CD denotes 4
T18102 1272-1274 IN denotes of
T18101 1265-1271 NN denotes volume
T18100 1263-1264 DT denotes a
T18099 1260-1262 IN denotes in
T18098 1251-1259 VBN denotes cultured
T18097 1246-1250 VBD denotes were
T18096 1241-1245 NNP denotes PBMC
T18095 1239-1240 , denotes ,
T18094 1230-1239 NNS denotes cytokines
T18093 1226-1229 CC denotes and
T18092 1218-1225 NNS denotes factors
T18091 1204-1217 NN denotes transcription
T18090 1201-1203 IN denotes of
T18089 1191-1200 NN denotes induction
T18088 1187-1190 IN denotes For
T18087 1176-1186 NNS denotes conditions
T18086 1164-1175 NN denotes Stimulation
T18598 2113-2120 NNP denotes Germany
T18597 2111-2112 , denotes ,
T18596 2099-2111 NNP denotes Braunschweig
T17922 1161-1162 . denotes .
T17921 1160-1161 -RRB- denotes )
T17920 1148-1160 NNP denotes BioWhittaker
T17919 1147-1148 -LRB- denotes (
T17918 1135-1146 NNP denotes L-glutamine
T17917 1132-1134 NNP denotes mM
T17916 1128-1131 CD denotes 200
T17915 1126-1127 NN denotes %
T17914 1125-1126 CD denotes 1
T17913 1121-1124 CC denotes and
T17912 1119-1120 , denotes ,
T17911 1118-1119 -RRB- denotes )
T17910 1116-1118 NN denotes ml
T17909 1115-1116 NN denotes /
T17908 1113-1115 NN denotes μg
T17907 1107-1112 CD denotes 10000
T17906 1106-1107 -LRB- denotes (
T17905 1093-1105 NN denotes streptomycin
T17904 1091-1092 NN denotes %
T17903 1090-1091 CD denotes 1
T17902 1088-1089 , denotes ,
T17901 1087-1088 -RRB- denotes )
T17900 1083-1087 NNP denotes U/ml
T17899 1077-1082 CD denotes 10000
T17898 1076-1077 -LRB- denotes (
T17897 1065-1075 NN denotes penicillin
T17896 1063-1064 NN denotes %
T17895 1062-1063 CD denotes 1
T17894 1060-1061 , denotes ,
T17893 1059-1060 -RRB- denotes )
T17892 1052-1059 NNP denotes Germany
T17891 1050-1051 , denotes ,
T17890 1041-1050 NNP denotes Karlsruhe
T17889 1039-1040 , denotes ,
T17888 1029-1039 NNP denotes Invitrogen
T17887 1027-1028 , denotes ,
T17886 1020-1027 NNP denotes low-LPS
T17885 1018-1019 , denotes ,
T17884 1015-1018 NNP denotes FCS
T17883 1014-1015 -LRB- denotes (
T17882 1008-1013 NN denotes serum
T17881 1003-1007 NN denotes calf
T17880 997-1002 JJ denotes fetal
T17879 980-996 JJ denotes heat-inactivated
T17878 978-979 NN denotes %
T17877 976-978 CD denotes 10
T17876 965-975 VBG denotes containing
T17875 963-964 , denotes ,
T17874 962-963 -RRB- denotes )
T17873 954-962 JJ denotes LPS-free
T17872 952-953 , denotes ,
T17871 945-952 NNP denotes Germany
T17870 943-944 , denotes ,
T17869 933-943 NNP denotes Heidelberg
T17868 931-932 , denotes ,
T17867 919-931 NNP denotes BioWhittaker
T17866 918-919 -LRB- denotes (
T17865 911-917 NN denotes medium
T17864 906-910 CD denotes 1640
T17863 901-905 NNP denotes RPMI
T17862 898-900 IN denotes in
T17861 896-897 -RRB- denotes )
T17860 887-896 NN denotes cytometry
T17859 882-886 NN denotes flow
T17858 878-881 IN denotes for
T17857 877-878 -LRB- denotes (
T17856 868-876 NN denotes cells/ml
T17855 864-867 CD denotes 106
T17854 862-863 CD denotes ×
T17853 860-861 CD denotes 1
T17852 857-859 CC denotes or
T17851 855-856 -RRB- denotes )
T17850 849-855 NNS denotes shifts
T17849 845-848 NN denotes gel
T17848 841-844 IN denotes for
T17847 840-841 -LRB- denotes (
T17846 833-839 CD denotes 106/ml
T17845 831-832 NN denotes ×
T17844 829-830 CD denotes 5
T17843 826-828 CC denotes or
T17842 824-825 -RRB- denotes )
T17841 812-824 NNS denotes measurements
T17840 806-811 NNP denotes ELISA
T17839 802-805 IN denotes for
T17838 801-802 -LRB- denotes (
T17837 792-800 NN denotes cells/ml
T17836 788-791 CD denotes 106
T17835 786-787 CD denotes ×
T17834 784-785 CD denotes 3
T17833 781-783 IN denotes of
T17832 767-780 NN denotes concentration
T17831 761-766 JJ denotes final
T17830 759-760 DT denotes a
T17829 756-758 IN denotes at
T17828 747-755 VBN denotes cultured
T17827 742-746 VBD denotes were
T17826 737-741 NNP denotes PBMC
T17825 735-736 , denotes ,
T17824 726-735 NN denotes isolation
T17823 716-725 VBG denotes Following
T17822 714-715 . denotes .
T17821 713-714 NNP denotes ]
T17820 708-713 CD denotes 54,55
T17819 707-708 NNP denotes [
T17818 698-706 NN denotes gradient
T17817 696-697 -RRB- denotes )
T17816 689-696 NNP denotes Germany
T17815 687-688 , denotes ,
T17814 681-687 NNP denotes Berlin
T17813 679-680 , denotes ,
T17812 671-679 NNP denotes Biochrom
T17811 670-671 -LRB- denotes (
T17810 655-669 NNP denotes Ficoll-Hypaque
T17809 653-654 DT denotes a
T17808 648-652 IN denotes over
T17807 638-647 VBN denotes separated
T17806 633-637 VBD denotes were
T17805 627-632 NNS denotes coats
T17804 621-626 JJ denotes buffy
T17803 617-620 DT denotes The
T17802 615-616 . denotes .
T17801 608-615 NNP denotes Luebeck
T17800 605-607 IN denotes of
T17799 594-604 NNP denotes University
T17798 590-593 DT denotes the
T17797 587-589 IN denotes of
T17796 577-586 NN denotes committee
T17795 570-576 NNS denotes ethics
T17794 566-569 DT denotes the
T17793 563-565 IN denotes of
T17792 554-562 NN denotes approval
T17611 418-419 . denotes .
T17610 407-418 NNS denotes 3-D-glucans
T17609 406-407 NN denotes →
T17608 403-406 JJ denotes β-1
T17607 400-402 TO denotes to
T17606 392-399 VB denotes respond
T17605 388-391 RB denotes not
T17604 383-387 VBZ denotes does
T17603 379-382 CC denotes but
T17602 369-378 NN denotes endotoxin
T17601 365-368 IN denotes for
T17600 356-364 JJ denotes specific
T17599 353-355 VBZ denotes is
T17598 347-352 WDT denotes which
T17597 345-346 , denotes ,
T17596 344-345 -RRB- denotes )
T17595 339-344 NNP denotes Japan
T17594 337-338 , denotes ,
T17593 332-337 NNP denotes Tokyo
T17592 330-331 , denotes ,
T17591 321-330 NNP denotes Seigakaku
T17590 320-321 -LRB- denotes (
T17589 314-319 NN denotes assay
T17588 304-313 NNP denotes Endospecy
T17587 300-303 DT denotes the
T17586 295-299 IN denotes with
T17585 281-294 NN denotes contamination
T17584 271-280 NN denotes endotoxin
T17583 267-270 IN denotes for
T17582 258-266 VBD denotes screened
T17581 254-257 CC denotes and
T17580 252-253 -RRB- denotes )
T17579 250-252 NN denotes μm
T17578 246-249 CD denotes 0.2
T17577 245-246 -LRB- denotes (
T17576 227-244 VBN denotes filter-sterilized
T18209 1775-1776 . denotes .
T17543 63-64 -LRB- denotes (
T17542 52-62 NN denotes 3-D-glucan
T17541 51-52 NN denotes →
T17540 48-51 JJ denotes β-1
T17539 33-47 VBN denotes phosphorylated
T17538 25-32 JJ denotes soluble
T17537 19-24 NNP denotes Water
T17536 9-18 NNPS denotes Materials
T17575 225-226 , denotes ,
T17574 220-225 NNS denotes media
T17573 212-219 JJ denotes aqueous
T17572 209-211 IN denotes in
T17571 199-208 VBN denotes dissolved
T17570 195-198 VBD denotes was
T17569 192-194 NNP denotes GP
T17568 190-191 . denotes .
T17567 189-190 NNP denotes ]
T17566 184-189 CD denotes 20,26
T17565 183-184 NNP denotes [
T17564 172-182 RB denotes previously
T17563 163-171 VBN denotes reported
T17562 160-162 IN denotes as
T17561 149-159 VBN denotes determined
T17560 144-148 VBD denotes were
T17559 141-143 NNP denotes GP
T17558 138-140 IN denotes of
T17557 122-137 NNS denotes characteristics
T17556 106-121 JJ denotes physicochemical
T17555 102-105 DT denotes The
T17554 100-101 . denotes .
T17553 99-100 NNP denotes ]
T17552 97-99 CD denotes 20
T17551 96-97 NNP denotes [
T17550 89-95 IN denotes before
T17549 84-88 VBN denotes done
T17548 81-83 IN denotes as
T17547 72-80 VBN denotes prepared
T17546 68-71 VBD denotes was
T17545 66-67 -RRB- denotes )
T17544 64-66 NNP denotes GP
T17791 550-553 DT denotes the
T17790 545-549 IN denotes with
T17789 541-544 CC denotes and
T17788 529-540 NN denotes declaration
T17787 520-528 NNP denotes Helsinki
T17786 516-519 DT denotes the
T17785 511-515 IN denotes with
T17784 500-510 NN denotes compliance
T17783 497-499 IN denotes in
T17782 490-496 NNS denotes donors
T17781 484-489 NN denotes blood
T17780 476-483 JJ denotes healthy
T17779 471-475 IN denotes from
T17778 462-470 VBN denotes obtained
T17777 457-461 VBD denotes were
T17776 451-456 NNS denotes coats
T17775 445-450 NNP denotes Buffy
T17774 440-444 NNP denotes PBMC
T17773 434-439 JJ denotes human
T17772 431-433 IN denotes of
T17771 421-430 NN denotes Isolation
T20724 6407-6410 NNP denotes CAG
T20723 6403-6406 NNP denotes CAA
T20722 6399-6402 NNP denotes TTA
T20721 6398-6399 -LRB- denotes (
T20720 6391-6397 NNP denotes NFIL-6
T20719 6387-6390 IN denotes for
T20718 6380-6386 NNS denotes oligos
T20717 6372-6379 VBN denotes mutated
T20716 6362-6371 VBG denotes including
T20715 6360-6361 , denotes ,
T20714 6354-6360 RB denotes before
T20713 6344-6353 VBN denotes described
T20712 6341-6343 IN denotes as
T20711 6338-6340 NNP denotes GP
T20710 6333-6337 IN denotes with
T20709 6322-6332 NN denotes incubation
T20708 6320-6321 NN denotes h
T20707 6318-6319 CD denotes 1
T20706 6312-6317 IN denotes after
T20705 6301-6311 VBN denotes determined
T20704 6296-6300 VBD denotes were
T20703 6289-6295 NNS denotes shifts
T20702 6284-6288 NNP denotes Band
T20743 6482-6485 NNP denotes TTA
T20742 6478-6481 NNP denotes TAT
T20741 6474-6477 NNP denotes AAA
T20740 6470-6473 NNP denotes GTA
T20739 6466-6469 NNP denotes AAG
T20738 6462-6465 NNP denotes GAA
T20737 6458-6461 NNP denotes GCA
T20736 6454-6457 NNP denotes GGC
T20735 6453-6454 -LRB- denotes (
T20734 6444-6452 NNP denotes NFATP2/3
T20733 6440-6443 CC denotes and
T20732 6438-6439 -RRB- denotes )
T20731 6435-6438 NNP denotes GGG
T20730 6431-6434 NNP denotes AGT
T20729 6427-6430 NNP denotes CTT
T20728 6423-6426 NNP denotes ACA
T20727 6419-6422 NNP denotes GCG
T20726 6415-6418 NNP denotes ATT
T20725 6411-6414 NNP denotes TGG
T20071 5198-5203 NNP denotes ELISA
T20070 5195-5197 DT denotes an
T20069 5192-5194 IN denotes by
T20068 5187-5191 NNP denotes IL-8
T20067 5183-5186 CC denotes and
T20066 5176-5182 NNP denotes IL-1RA
T20065 5174-5175 , denotes ,
T20064 5173-5174 -RRB- denotes )
T20063 5166-5173 NNP denotes Austria
T20062 5164-5165 , denotes ,
T20061 5158-5164 NNP denotes Vienna
T20060 5157-5158 -LRB- denotes (
T20059 5149-5156 NNP denotes Systems
T20084 5266-5271 VBG denotes using
T20083 5264-5265 CD denotes 1
T20082 5259-5263 NNP denotes TGFβ
T20081 5255-5258 CC denotes and
T20080 5253-5254 , denotes ,
T20079 5252-5253 -RRB- denotes )
T20078 5245-5252 NNP denotes Germany
T20077 5243-5244 , denotes ,
T20076 5222-5243 NNP denotes Wiesbaden-Nordenstadt
T20075 5221-5222 -LRB- denotes (
T20074 5213-5220 NNP denotes Systems
T20073 5209-5212 NNP denotes R&D
T20072 5204-5208 IN denotes from
T20168 5713-5715 IN denotes in
T20167 5704-5712 VBD denotes observed
T20166 5699-5703 NNP denotes PBMC
T20165 5696-5698 IN denotes of
T20164 5684-5695 NN denotes stimulation
T20163 5679-5683 RB denotes Only
T20162 5677-5678 . denotes .
T20161 5672-5677 NN denotes curve
T20160 5663-5671 JJ denotes standard
T20159 5659-5662 DT denotes the
T20158 5656-5658 IN denotes of
T20157 5650-5655 NN denotes range
R11837 T17536 T17547 nsubjpass Materials,prepared
R11838 T17537 T17542 nmod Water,3-D-glucan
R11839 T17538 T17539 amod soluble,phosphorylated
R11840 T17539 T17542 amod phosphorylated,3-D-glucan
R11841 T17540 T17542 amod β-1,3-D-glucan
R11842 T17541 T17542 compound →,3-D-glucan
R11843 T17542 T17542 ROOT 3-D-glucan,3-D-glucan
R11844 T17543 T17544 punct (,GP
R11845 T17544 T17542 appos GP,3-D-glucan
R11846 T17545 T17542 punct ),3-D-glucan
R11847 T17546 T17547 auxpass was,prepared
R11848 T17547 T17547 ROOT prepared,prepared
R11849 T17548 T17549 mark as,done
R11850 T17549 T17547 advcl done,prepared
R11851 T17550 T17549 advmod before,done
R11852 T17551 T17553 nmod [,]
R11853 T17552 T17551 nummod 20,[
R11854 T17553 T17550 pobj ],before
R11876 T17575 T17574 punct ",",media
R11877 T17576 T17571 conj filter-sterilized,dissolved
R11878 T17577 T17579 punct (,μm
R11879 T17578 T17579 nummod 0.2,μm
R11880 T17579 T17576 appos μm,filter-sterilized
R11881 T17580 T17579 punct ),μm
R11882 T17581 T17576 cc and,filter-sterilized
R11883 T17582 T17576 conj screened,filter-sterilized
R11884 T17583 T17582 prep for,screened
R11885 T17584 T17585 compound endotoxin,contamination
R11886 T17585 T17583 pobj contamination,for
R11887 T17586 T17585 prep with,contamination
R11888 T17587 T17589 det the,assay
R11889 T17588 T17589 compound Endospecy,assay
R11890 T17589 T17586 pobj assay,with
R11891 T17590 T17589 punct (,assay
R11892 T17591 T17589 appos Seigakaku,assay
R11893 T17592 T17591 punct ",",Seigakaku
R11894 T17593 T17606 nsubj Tokyo,respond
R11895 T17594 T17593 punct ",",Tokyo
R11896 T17595 T17593 conj Japan,Tokyo
R11897 T17596 T17595 punct ),Japan
R11898 T17597 T17593 punct ",",Tokyo
R11899 T17598 T17599 nsubj which,is
R11900 T17599 T17593 relcl is,Tokyo
R11901 T17600 T17599 acomp specific,is
R11902 T17601 T17600 prep for,specific
R11903 T17602 T17601 pobj endotoxin,for
R11904 T17603 T17599 cc but,is
R11905 T17604 T17606 aux does,respond
R11906 T17605 T17606 neg not,respond
R11907 T17606 T17571 conj respond,dissolved
R11908 T17607 T17606 prep to,respond
R11909 T17608 T17610 amod β-1,3-D-glucans
R11910 T17609 T17610 compound →,3-D-glucans
R11911 T17610 T17607 pobj 3-D-glucans,to
R11912 T17611 T17606 punct .,respond
R12086 T17795 T17796 compound ethics,committee
R12087 T17796 T17793 pobj committee,of
R12088 T17797 T17796 prep of,committee
R12089 T17798 T17799 det the,University
R12090 T17799 T17797 pobj University,of
R12091 T17800 T17799 prep of,University
R12092 T17801 T17800 pobj Luebeck,of
R12093 T17802 T17778 punct .,obtained
R12094 T17803 T17805 det The,coats
R12095 T17804 T17805 amod buffy,coats
R12096 T17805 T17807 nsubjpass coats,separated
R12097 T17806 T17807 auxpass were,separated
R12098 T17807 T17807 ROOT separated,separated
R12099 T17808 T17807 prep over,separated
R12100 T17809 T17810 det a,Ficoll-Hypaque
R12101 T17810 T17808 pobj Ficoll-Hypaque,over
R12102 T17811 T17818 punct (,gradient
R12103 T17812 T17818 nmod Biochrom,gradient
R12104 T17813 T17812 punct ",",Biochrom
R12105 T17814 T17812 conj Berlin,Biochrom
R12106 T17815 T17814 punct ",",Berlin
R12107 T17816 T17814 conj Germany,Berlin
R12108 T17817 T17818 punct ),gradient
R12145 T17854 T17856 nummod ×,cells/ml
R12146 T17855 T17856 nummod 106,cells/ml
R12147 T17856 T17845 conj cells/ml,×
R12148 T17857 T17858 punct (,for
R12149 T17858 T17856 prep for,cells/ml
R12150 T17859 T17860 compound flow,cytometry
R12151 T17860 T17858 pobj cytometry,for
R12182 T17891 T17890 punct ",",Karlsruhe
R12183 T17892 T17890 conj Germany,Karlsruhe
R12184 T17893 T17890 punct ),Karlsruhe
R12185 T17894 T17888 punct ",",Invitrogen
R12186 T17895 T17896 nummod 1,%
R12187 T17896 T17897 compound %,penicillin
R12188 T17897 T17888 appos penicillin,Invitrogen
R12189 T17898 T17900 punct (,U/ml
R12190 T17899 T17900 nummod 10000,U/ml
R12191 T17900 T17897 appos U/ml,penicillin
R12192 T17901 T17900 punct ),U/ml
R12193 T17902 T17897 punct ",",penicillin
R12194 T17903 T17904 nummod 1,%
R12195 T17904 T17905 compound %,streptomycin
R12196 T17905 T17882 appos streptomycin,serum
R12197 T17906 T17905 punct (,streptomycin
R12198 T17907 T17910 nummod 10000,ml
R12199 T17908 T17910 compound μg,ml
R12200 T17909 T17910 compound /,ml
R12201 T17910 T17905 appos ml,streptomycin
R12202 T17911 T17905 punct ),streptomycin
R12203 T17912 T17905 punct ",",streptomycin
R12204 T17913 T17882 cc and,serum
R12205 T17914 T17915 nummod 1,%
R12206 T17915 T17876 npadvmod %,containing
R12207 T17916 T17918 nummod 200,L-glutamine
R12208 T17917 T17918 compound mM,L-glutamine
R12209 T17918 T17918 ROOT L-glutamine,L-glutamine
R12210 T17919 T17920 punct (,BioWhittaker
R12211 T17920 T17918 appos BioWhittaker,L-glutamine
R12212 T17921 T17918 punct ),L-glutamine
R12213 T17922 T17828 punct .,cultured
R12338 T18086 T18087 compound Stimulation,conditions
R12339 T18087 T18098 nsubjpass conditions,cultured
R12340 T18088 T18098 prep For,cultured
R12341 T18089 T18088 pobj induction,For
R12342 T18090 T18089 prep of,induction
R12343 T18091 T18092 compound transcription,factors
R12344 T18092 T18090 pobj factors,of
R12345 T18093 T18092 cc and,factors
R12346 T18094 T18092 conj cytokines,factors
R12347 T18095 T18098 punct ",",cultured
R12348 T18096 T18098 nsubjpass PBMC,cultured
R12349 T18097 T18098 auxpass were,cultured
R12350 T18098 T18098 ROOT cultured,cultured
R12351 T18099 T18098 prep in,cultured
R12352 T18100 T18101 det a,volume
R12353 T18101 T18099 pobj volume,in
R12354 T18102 T18101 prep of,volume
R12355 T18103 T18104 nummod 4,ml
R12356 T18104 T18102 pobj ml,of
R12357 T18105 T18101 prep in,volume
R12358 T18106 T18110 amod sterile,plates
R12359 T18107 T18110 amod pyrogen-free,plates
R12360 T18108 T18110 amod 6-well,plates
R12361 T18109 T18110 compound culture,plates
R12362 T18110 T18105 pobj plates,in
R12363 T18111 T18117 punct (,)
R12364 T18112 T18117 nmod Falcon,)
R12365 T18113 T18112 punct ",",Falcon
R12366 T18114 T18112 conj Heidelberg,Falcon
R12367 T18115 T18114 punct ",",Heidelberg
R12368 T18116 T18112 conj Germany,Falcon
R12369 T18117 T18098 punct ),cultured
R12370 T18118 T18098 punct .,cultured
R12371 T18119 T18121 nsubjpass Cultures,stimulated
R12372 T18120 T18121 auxpass were,stimulated
R12373 T18121 T18121 ROOT stimulated,stimulated
R12374 T18122 T18121 prep for,stimulated
R12375 T18123 T18124 nummod 1,h
R12376 T18124 T18122 pobj h,for
R12377 T18125 T18124 punct (,h
R12378 T18126 T18124 appos EMSA,h
R12379 T18127 T18126 punct ),EMSA
R12380 T18128 T18122 cc or,for
R12381 T18129 T18121 prep from,stimulated
R12382 T18130 T18133 quantmod 0,48
R12383 T18131 T18133 quantmod h,48
R12384 T18132 T18133 quantmod to,48
R12385 T18133 T18134 nummod 48,h
R12386 T18134 T18129 pobj h,from
R12387 T18135 T18134 punct (,h
R12388 T18136 T18134 appos ELISA,h
R12389 T18137 T18134 punct ),h
R12390 T18138 T18121 prep with,stimulated
R12391 T18139 T18142 nummod 1,μg
R12392 T18140 T18139 cc or,1
R12393 T18141 T18139 conj 100,1
R12394 T18142 T18138 pobj μg,with
R12395 T18143 T18142 prep per,μg
R12396 T18144 T18145 nummod 106,cells
R12397 T18145 T18143 pobj cells,per
R12398 T18146 T18145 prep of,cells
R12399 T18147 T18146 pobj GP,of
R12400 T18148 T18121 punct ",",stimulated
R12401 T18149 T18121 prep with,stimulated
R12402 T18150 T18152 nummod 250,cells
R12403 T18151 T18152 nummod ng/106,cells
R12404 T18152 T18149 pobj cells,with
R12405 T18153 T18173 nmod TSST-1,coli
R12406 T18154 T18156 punct (,Technologies
R12407 T18155 T18156 compound Toxin,Technologies
R12408 T18156 T18153 appos Technologies,TSST-1
R12409 T18157 T18156 punct ",",Technologies
R12410 T18158 T18156 conj Sarasota,Technologies
R12411 T18159 T18158 punct ",",Sarasota
R12412 T18160 T18158 conj FL,Sarasota
R12413 T18161 T18160 punct ",",FL
R12414 T18162 T18160 npadvmod USA,FL
R12415 T18163 T18160 punct ),FL
R12416 T18164 T18156 cc or,Technologies
R12417 T18165 T18153 prep with,TSST-1
R12418 T18166 T18168 nummod 250,cells
R12419 T18167 T18168 nummod ng/106,cells
R12420 T18168 T18165 pobj cells,with
R12421 T18169 T18170 compound wild-type,LPS
R12422 T18170 T18153 conj LPS,TSST-1
R12423 T18171 T18153 prep from,TSST-1
R12424 T18172 T18173 compound Escherichia,coli
R12425 T18173 T18174 nsubj coli,serotype
R12426 T18174 T18121 advcl serotype,stimulated
R12427 T18175 T18174 nummod 0111,serotype
R12428 T18176 T18174 punct :,serotype
R12429 T18177 T18179 nmod B4,Sigma
R12430 T18178 T18179 punct (,Sigma
R12431 T18179 T18174 appos Sigma,serotype
R12432 T18180 T18179 punct ",",Sigma
R12433 T18181 T18179 conj Munich,Sigma
R12434 T18182 T18181 punct ",",Munich
R12435 T18183 T18181 conj Germany,Munich
R12436 T18184 T18179 punct ),Sigma
R12437 T18185 T18121 punct .,stimulated
R12438 T18186 T18192 prep In,supplemented
R12439 T18187 T18188 amod costimulatory,experiments
R12440 T18188 T18186 pobj experiments,In
R12441 T18189 T18192 punct ",",supplemented
R12442 T18190 T18192 nsubjpass PBMC,supplemented
R12443 T18191 T18192 auxpass were,supplemented
R12444 T18192 T18192 ROOT supplemented,supplemented
R12445 T18193 T18192 advmod simultaneously,supplemented
R12446 T18194 T18192 prep with,supplemented
R12447 T18195 T18196 det a,combination
R12448 T18196 T18194 pobj combination,with
R12449 T18197 T18196 prep of,combination
R12450 T18198 T18197 pobj GP,of
R12451 T18199 T18198 cc and,GP
R12452 T18200 T18198 conj TSST-1,GP
R12453 T18201 T18194 cc or,with
R12454 T18202 T18194 conj with,with
R12455 T18203 T18204 det a,combination
R12456 T18204 T18202 pobj combination,with
R12457 T18205 T18204 prep of,combination
R12458 T18206 T18205 pobj GP,of
R12459 T18207 T18206 cc and,GP
R12460 T18208 T18206 conj LPS,GP
R12461 T18209 T18192 punct .,supplemented
R12666 T18530 T18530 ROOT Oligonucleotides,Oligonucleotides
R12667 T18531 T18532 punct (,Oligos
R12668 T18532 T18530 appos Oligos,Oligonucleotides
R12669 T18533 T18532 punct ),Oligos
R12670 T18534 T18530 cc and,Oligonucleotides
R12671 T18535 T18539 nummod 5,P-Labeling
R12672 T18536 T18537 punct ',-32
R12673 T18537 T18539 punct -32,P-Labeling
R12674 T18538 T18539 punct -,P-Labeling
R12675 T18539 T18530 conj P-Labeling,Oligonucleotides
R12676 T18540 T18542 det The,double-stranded
R12677 T18541 T18542 amod complementary,double-stranded
R12678 T18542 T18615 nsubj double-stranded,according
R12679 T18543 T18544 punct (,ds
R12680 T18544 T18561 nsubjpass ds,synthesised
R12681 T18545 T18544 punct ),ds
R12682 T18546 T18544 appos oligonucleotides,ds
R12683 T18547 T18548 punct (,oligos
R12684 T18548 T18546 appos oligos,oligonucleotides
R12685 T18549 T18546 punct ),oligonucleotides
R12686 T18550 T18544 prep from,ds
R12687 T18551 T18552 det the,TNFα
R12688 T18552 T18550 pobj TNFα,from
R12689 T18553 T18552 punct ",",TNFα
R12690 T18554 T18552 conj IL-8,TNFα
R12691 T18555 T18554 punct ",",IL-8
R12692 T18556 T18554 conj IFNγ,IL-8
R12693 T18557 T18556 cc and,IFNγ
R12694 T18558 T18556 conj IL-6,IFNγ
R12695 T18559 T18550 pobj promoters,from
R12696 T18560 T18561 auxpass were,synthesised
R12697 T18561 T18542 relcl synthesised,double-stranded
R12698 T18562 T18561 prep from,synthesised
R12699 T18563 T18564 amod single,stranded
R12700 T18564 T18561 conj stranded,synthesised
R12701 T18565 T18566 punct (,ss
R12702 T18566 T18564 appos ss,stranded
R12703 T18567 T18566 punct ),ss
R12704 T18568 T18566 appos oligos,ss
R12705 T18569 T18570 punct (,illustrated
R12706 T18570 T18568 acl illustrated,oligos
R12707 T18571 T18570 prep in,illustrated
R12708 T18572 T18571 pobj Table,in
R12709 T18573 T18572 nummod 1,Table
R12710 T18574 T18566 punct ),ss
R12711 T18575 T18564 punct ",",stranded
R12712 T18576 T18561 punct (,synthesised
R12713 T18577 T18578 compound TIB,Molbiol
R12714 T18578 T18600 nsubj Molbiol,using
R12715 T18579 T18578 punct ",",Molbiol
R12716 T18580 T18578 conj Berlin,Molbiol
R12717 T18581 T18580 punct ",",Berlin
R12718 T18582 T18580 conj Germany,Berlin
R12719 T18583 T18582 punct ),Germany
R12720 T18584 T18578 cc and,Molbiol
R12721 T18585 T18578 conj 32P-labeled,Molbiol
R12722 T18586 T18585 prep with,32P-labeled
R12723 T18587 T18589 nummod 5,γ-P-ATP
R12724 T18588 T18589 punct ',γ-P-ATP
R12725 T18589 T18586 pobj γ-P-ATP,with
R12726 T18590 T18592 punct (,Ci/mmol
R12727 T18591 T18592 nummod "3,000",Ci/mmol
R12728 T18592 T18589 appos Ci/mmol,γ-P-ATP
R12729 T18593 T18592 punct ",",Ci/mmol
R12730 T18594 T18592 conj Amersham,Ci/mmol
R12731 T18595 T18594 punct ",",Amersham
R12732 T18596 T18594 conj Braunschweig,Amersham
R12733 T18597 T18596 punct ",",Braunschweig
R12734 T18598 T18596 conj Germany,Braunschweig
R12735 T18599 T18589 punct ),γ-P-ATP
R12736 T18600 T18561 advcl using,synthesised
R12737 T18601 T18603 det the,Kit
R12738 T18602 T18603 compound Ready-To-Go-Polynucleotide-Kinase,Kit
R12739 T18603 T18600 dobj Kit,using
R12740 T18604 T18606 punct (,Kit
R12741 T18605 T18606 compound PNK,Kit
R12742 T18606 T18603 appos Kit,Kit
R12743 T18607 T18606 punct ",",Kit
R12744 T18608 T18609 compound Pharmacia,LKB
R12745 T18609 T18606 conj LKB,Kit
R12746 T18610 T18609 punct ",",LKB
R12747 T18611 T18609 conj Freiburg,LKB
R12748 T18612 T18611 punct ",",Freiburg
R12749 T18613 T18611 conj Germany,Freiburg
R12750 T18614 T18603 punct ),Kit
R12751 T18615 T18615 ROOT according,according
R12752 T18616 T18615 prep to,according
R12753 T18617 T18618 det the,manufacturer
R12754 T18618 T18620 poss manufacturer,instructions
R12755 T18619 T18618 case 's,manufacturer
R12756 T18620 T18616 pobj instructions,to
R12757 T18621 T18615 punct .,according
R12758 T18622 T18623 aux To,test
R12759 T18623 T18634 advcl test,used
R12760 T18624 T18626 det the,specificity
R12761 T18625 T18626 amod binding,specificity
R12762 T18626 T18623 dobj specificity,test
R12763 T18627 T18626 prep of,specificity
R12764 T18628 T18629 det the,oligos
R12765 T18629 T18627 pobj oligos,of
R12766 T18630 T18634 punct ",",used
R12767 T18631 T18632 amod mutated,oligos
R12768 T18632 T18634 nsubjpass oligos,used
R12769 T18633 T18634 auxpass were,used
R12770 T18634 T18634 ROOT used,used
R12771 T18635 T18634 prep as,used
R12772 T18636 T18638 det an,control
R12773 T18637 T18638 amod additional,control
R12774 T18638 T18635 pobj control,as
R12775 T18639 T18634 punct .,used
R12776 T18640 T18667 nsubjpass Mutations,reported
R12777 T18641 T18640 prep in,Mutations
R12778 T18642 T18644 det the,site
R12779 T18643 T18644 compound P2,site
R12780 T18644 T18641 pobj site,in
R12781 T18645 T18644 prep of,site
R12782 T18646 T18648 det the,promoter
R12783 T18647 T18648 compound IFNγ,promoter
R12784 T18648 T18645 pobj promoter,of
R12785 T18649 T18651 nmod [,]
R12786 T18650 T18651 nummod 29,]
R12787 T18651 T18648 appos ],promoter
R12788 T18652 T18641 cc and,in
R12789 T18653 T18641 conj in,in
R12790 T18654 T18657 det the,sequence
R12791 T18655 T18657 compound κ,sequence
R12792 T18656 T18657 compound consensus,sequence
R12793 T18657 T18653 pobj sequence,in
R12794 T18658 T18657 prep of,sequence
R12795 T18659 T18661 det the,promoter
R12796 T18660 T18661 compound TNFα,promoter
R12797 T18661 T18658 pobj promoter,of
R12798 T18662 T18664 nmod [,]
R12799 T18663 T18664 nummod 55,]
R12800 T18664 T18661 appos ],promoter
R12801 T18665 T18667 aux have,reported
R12802 T18666 T18667 auxpass been,reported
R12803 T18667 T18667 ROOT reported,reported
R12804 T18668 T18669 aux to,interfere
R12805 T18669 T18667 xcomp interfere,reported
R12806 T18670 T18669 prep with,interfere
R12807 T18671 T18670 pobj sequences,with
R12808 T18672 T18671 punct ",",sequences
R12809 T18673 T18674 nsubj which,seem
R12810 T18674 T18671 relcl seem,sequences
R12811 T18675 T18676 aux to,be
R12812 T18676 T18674 xcomp be,seem
R12813 T18677 T18676 acomp crucial,be
R12814 T18678 T18677 prep for,crucial
R12815 T18679 T18680 det the,binding
R12816 T18680 T18678 pobj binding,for
R12817 T18681 T18680 prep of,binding
R12818 T18682 T18685 nmod NFAT,proteins
R12819 T18683 T18682 cc and,NFAT
R12820 T18684 T18682 conj NFκB,NFAT
R12821 T18685 T18681 pobj proteins,of
R12822 T18686 T18676 punct ",",be
R12823 T18687 T18676 advmod respectively,be
R12824 T18688 T18667 punct .,reported
R12825 T18689 T18695 advmod Afterwards,purified
R12826 T18690 T18695 punct ",",purified
R12827 T18691 T18693 det the,oligos
R12828 T18692 T18693 compound ds,oligos
R12829 T18693 T18695 nsubjpass oligos,purified
R12830 T18694 T18695 auxpass were,purified
R12831 T18695 T18695 ROOT purified,purified
R12832 T18696 T18695 prep via,purified
R12833 T18697 T18698 compound gel,filtration
R12834 T18698 T18696 pobj filtration,via
R12835 T18699 T18695 advcl using,purified
R12836 T18700 T18701 compound Probe,Quant
R12837 T18701 T18704 compound Quant,Columns
R12838 T18702 T18704 compound G-50,Columns
R12839 T18703 T18704 compound Micro,Columns
R12840 T18704 T18699 dobj Columns,using
R12841 T18705 T18704 punct (,Columns
R12842 T18706 T18704 appos Pharmacia,Columns
R12843 T18707 T18704 punct ),Columns
R12844 T18708 T18695 prep according,purified
R12845 T18709 T18708 prep to,according
R12846 T18710 T18711 det the,manufacturer
R12847 T18711 T18713 poss manufacturer,instructions
R12848 T18712 T18711 case 's,manufacturer
R12849 T18713 T18709 pobj instructions,to
R12850 T18714 T18695 punct .,purified
R13021 T18980 T18980 ROOT Preparation,Preparation
R13022 T18981 T18980 prep of,Preparation
R13023 T18982 T18983 amod nuclear,extracts
R13024 T18983 T18981 pobj extracts,of
R13025 T18984 T18983 cc and,extracts
R13026 T18985 T18987 amod electrophoretic,shift
R13027 T18986 T18987 compound mobility,shift
R13028 T18987 T18988 compound shift,assays
R13029 T18988 T18983 conj assays,extracts
R13030 T18989 T18993 punct (,extracts
R13031 T18990 T18993 nmod EMSA,extracts
R13032 T18991 T18990 punct ),EMSA
R13033 T18992 T18993 compound Nuclear,extracts
R13034 T18993 T18980 appos extracts,Preparation
R13035 T18994 T18993 prep of,extracts
R13036 T18995 T18996 amod purified,PBMCs
R13037 T18996 T18994 pobj PBMCs,of
R13038 T18997 T18980 auxpass were,Preparation
R13039 T18998 T18997 acomp prepared,were
R13040 T18999 T18997 prep according,were
R13041 T19000 T18999 prep to,according
R13042 T19001 T19002 det a,technique
R13043 T19002 T19000 pobj technique,to
R13044 T19003 T19002 acl described,technique
R13045 T19004 T19003 agent by,described
R13046 T19005 T19004 pobj Trede,by
R13047 T19006 T19010 nmod et,]
R13048 T19007 T19008 nmod al.,[
R13049 T19008 T19010 nmod [,]
R13050 T19009 T19010 nummod 27,]
R13051 T19010 T19002 appos ],technique
R13052 T19011 T18999 cc and,according
R13053 T19012 T18999 conj modified,according
R13054 T19013 T19012 prep for,modified
R13055 T19014 T19016 poss our,setup
R13056 T19015 T19016 amod experimental,setup
R13057 T19016 T19013 pobj setup,for
R13058 T19017 T19019 nmod [,]
R13059 T19018 T19019 nummod 56,]
R13060 T19019 T19016 appos ],setup
R13061 T19020 T18997 punct .,were
R13062 T19021 T19022 det The,intensity
R13063 T19022 T19026 nsubj intensity,was
R13064 T19023 T19022 prep of,intensity
R13065 T19024 T19025 amod shifted,bands
R13066 T19025 T19023 pobj bands,of
R13067 T19026 T19026 ROOT was,was
R13068 T19027 T19026 acomp normalized,was
R13069 T19028 T19027 prep to,normalized
R13070 T19029 T19028 pobj bands,to
R13071 T19030 T19029 prep of,bands
R13072 T19031 T19032 amod unstimulated,controls
R13073 T19032 T19030 pobj controls,of
R13074 T19033 T19026 punct .,was
R13075 T19034 T19035 compound Competition,experiments
R13076 T19035 T19035 ROOT experiments,experiments
R13077 T19036 T19035 prep with,experiments
R13078 T19037 T19036 pobj labeled,with
R13079 T19038 T19037 cc and,labeled
R13080 T19039 T19040 amod unlabeled,mutated
R13081 T19040 T19037 conj mutated,labeled
R13082 T19041 T19040 dobj oligos,mutated
R13083 T19042 T19041 prep of,oligos
R13084 T19043 T19045 det the,promoter
R13085 T19044 T19045 compound cytokine,promoter
R13086 T19045 T19047 nmod promoter,sites
R13087 T19046 T19047 amod binding,sites
R13088 T19047 T19042 pobj sites,of
R13089 T19048 T19049 punct (,see
R13090 T19049 T19041 parataxis see,oligos
R13091 T19050 T19049 advmod above,see
R13092 T19051 T19049 punct ),see
R13093 T19052 T19053 auxpass were,carried
R13094 T19053 T19037 conj carried,labeled
R13095 T19054 T19053 prt out,carried
R13096 T19055 T19053 prep in,carried
R13097 T19056 T19055 pobj order,in
R13098 T19057 T19058 aux to,prevent
R13099 T19058 T19056 acl prevent,order
R13100 T19059 T19060 amod non-specific,binding
R13101 T19060 T19058 dobj binding,prevent
R13102 T19061 T19060 prep to,binding
R13103 T19062 T19063 amod nuclear,proteins
R13104 T19063 T19061 pobj proteins,to
R13105 T19064 T19035 punct .,experiments
R13106 T19065 T19080 prep For,added
R13107 T19066 T19067 amod such,experiments
R13108 T19067 T19065 pobj experiments,For
R13109 T19068 T19080 punct ",",added
R13110 T19069 T19080 det an,added
R13111 T19070 T19080 nsubjpass excess,added
R13112 T19071 T19074 punct (,×
R13113 T19072 T19074 nummod 5,×
R13114 T19073 T19072 prep 50,5
R13115 T19074 T19070 appos ×,excess
R13116 T19075 T19074 punct ),×
R13117 T19076 T19074 prep of,×
R13118 T19077 T19078 amod unlabeled,oligos
R13119 T19078 T19076 pobj oligos,of
R13120 T19079 T19080 auxpass was,added
R13121 T19080 T19080 ROOT added,added
R13122 T19081 T19080 prep to,added
R13123 T19082 T19084 det the,extracts
R13124 T19083 T19084 amod nuclear,extracts
R13125 T19084 T19081 pobj extracts,to
R13126 T19085 T19080 punct .,added
R13127 T19086 T19101 prep For,performed
R13128 T19087 T19086 pobj specificity,For
R13129 T19088 T19087 prep of,specificity
R13130 T19089 T19092 amod binding,assays
R13131 T19090 T19092 punct ",",assays
R13132 T19091 T19092 amod supershift,assays
R13133 T19092 T19088 pobj assays,of
R13134 T19093 T19087 prep for,specificity
R13135 T19094 T19093 pobj NFκB,for
R13136 T19095 T19087 prep with,specificity
R13137 T19096 T19099 det a,antibody
R13138 T19097 T19099 amod combined,antibody
R13139 T19098 T19099 nummod p65/50,antibody
R13140 T19099 T19095 pobj antibody,with
R13141 T19100 T19101 auxpass were,performed
R13142 T19101 T19101 ROOT performed,performed
R13143 T19102 T19105 punct (,shown
R13144 T19103 T19105 nsubjpass data,shown
R13145 T19104 T19105 neg not,shown
R13146 T19105 T19101 parataxis shown,performed
R13147 T19106 T19105 punct ),shown
R13148 T19107 T19101 punct .,performed
R13149 T19108 T19109 aux To,exclude
R13150 T19109 T19128 advcl exclude,used
R13151 T19110 T19111 amod non-specific,reactions
R13152 T19111 T19109 dobj reactions,exclude
R13153 T19112 T19111 punct ",",reactions
R13154 T19113 T19116 det a,excess
R13155 T19114 T19116 amod 30-fold,excess
R13156 T19115 T19116 compound molar,excess
R13157 T19116 T19128 nsubjpass excess,used
R13158 T19117 T19116 prep of,excess
R13159 T19118 T19119 compound Oct-1,DNA
R13160 T19119 T19117 pobj DNA,of
R13161 T19120 T19119 punct (,DNA
R13162 T19121 T19122 amod unrelated,DNA
R13163 T19122 T19119 appos DNA,DNA
R13164 T19123 T19119 punct ",",DNA
R13165 T19124 T19119 appos Table,DNA
R13166 T19125 T19124 nummod 1,Table
R13167 T19126 T19119 punct ),DNA
R13168 T19127 T19128 auxpass was,used
R13169 T19128 T19128 ROOT used,used
R13170 T19129 T19128 punct ",",used
R13171 T19130 T19133 nsubj which,compete
R13172 T19131 T19133 aux did,compete
R13173 T19132 T19133 neg not,compete
R13174 T19133 T19128 relcl compete,used
R13175 T19134 T19133 prep with,compete
R13176 T19135 T19136 amod specific,binding
R13177 T19136 T19134 pobj binding,with
R13178 T19137 T19128 punct .,used
R13277 T19396 T19399 punct (,blot
R13278 T19397 T19399 nmod Dot,blot
R13279 T19398 T19399 punct ),blot
R13280 T19399 T19395 appos blot,Immuno
R13281 T19400 T19401 aux To,determine
R13282 T19401 T19478 ccomp determine,extract
R13283 T19402 T19408 mark whether,related
R13284 T19403 T19406 det the,proteins
R13285 T19404 T19406 nmod DNA,proteins
R13286 T19405 T19406 amod binding,proteins
R13287 T19406 T19408 nsubjpass proteins,related
R13288 T19407 T19408 auxpass were,related
R13289 T19408 T19401 ccomp related,determine
R13290 T19409 T19408 prep to,related
R13291 T19410 T19409 pobj NFκB,to
R13292 T19411 T19410 cc and,NFκB
R13293 T19412 T19410 conj NFAT,NFκB
R13294 T19413 T19401 punct ",",determine
R13295 T19414 T19478 ccomp dot,extract
R13296 T19415 T19414 dobj blots,dot
R13297 T19416 T19414 advcl using,dot
R13298 T19417 T19420 det a,minifold
R13299 T19418 T19420 compound SRC96D,minifold
R13300 T19419 T19420 compound SNS,minifold
R13301 T19420 T19416 dobj minifold,using
R13302 T19421 T19422 nsubj I,dot
R13303 T19422 T19478 ccomp dot,extract
R13304 T19423 T19422 dobj blotter,dot
R13305 T19424 T19434 punct (,performed
R13306 T19425 T19434 nsubjpass Schleicher,performed
R13307 T19426 T19425 cc &,Schleicher
R13308 T19427 T19425 conj Schüll,Schleicher
R13309 T19428 T19427 punct ",",Schüll
R13310 T19429 T19427 conj Dassel,Schüll
R13311 T19430 T19429 punct ",",Dassel
R13312 T19431 T19429 conj Germany,Dassel
R13313 T19432 T19434 punct ),performed
R13314 T19433 T19434 auxpass were,performed
R13315 T19434 T19478 ccomp performed,extract
R13316 T19435 T19434 prep with,performed
R13317 T19436 T19439 det the,controls
R13318 T19437 T19439 amod following,controls
R13319 T19438 T19439 amod positive,controls
R13320 T19439 T19435 pobj controls,with
R13321 T19440 T19439 cc and,controls
R13322 T19441 T19439 conj antibodies,controls
R13323 T19442 T19439 punct (,controls
R13324 T19443 T19444 advmod all,from
R13325 T19444 T19439 prep from,controls
R13326 T19445 T19446 compound Santa,Cruz
R13327 T19446 T19447 compound Cruz,Biotechnology
R13328 T19447 T19444 pobj Biotechnology,from
R13329 T19448 T19447 punct ",",Biotechnology
R13330 T19449 T19447 conj Heidelberg,Biotechnology
R13331 T19450 T19449 punct ",",Heidelberg
R13332 T19451 T19447 appos Germany,Biotechnology
R13333 T19452 T19439 punct ),controls
R13334 T19453 T19439 punct :,controls
R13335 T19454 T19455 amod positive,controls
R13336 T19455 T19439 appos controls,controls
R13337 T19456 T19455 punct (,controls
R13338 T19457 T19458 nummod 10,ng/dot
R13339 T19458 T19455 appos ng/dot,controls
R13340 T19459 T19455 punct ),controls
R13341 T19460 T19439 punct :,controls
R13342 T19461 T19462 compound NFκB,p52
R13343 T19462 T19439 appos p52,controls
R13344 T19463 T19465 punct (,kD
R13345 T19464 T19465 nummod 80,kD
R13346 T19465 T19462 appos kD,p52
R13347 T19466 T19439 punct ),controls
R13348 T19467 T19434 dobj sc-4095WB,performed
R13349 T19468 T19467 punct ",",sc-4095WB
R13350 T19469 T19467 appos c-Rel,sc-4095WB
R13351 T19470 T19472 punct (,kD
R13352 T19471 T19472 nummod 61,kD
R13353 T19472 T19469 appos kD,c-Rel
R13354 T19473 T19472 punct ),kD
R13355 T19474 T19469 amod sc-4030WB,c-Rel
R13356 T19475 T19478 punct ;,extract
R13357 T19476 T19478 nsubj Jurkat,extract
R13358 T19477 T19478 amod nuclear,extract
R13359 T19478 T19478 ROOT extract,extract
R13360 T19479 T19478 punct ",",extract
R13361 T19480 T19478 dep PMA-stimulated,extract
R13362 T19481 T19478 punct ;,extract
R13363 T19482 T19483 amod primary,antibodies
R13364 T19483 T19478 dobj antibodies,extract
R13365 T19484 T19483 punct (,antibodies
R13366 T19485 T19486 nummod 1,μg
R13367 T19486 T19488 compound μg,dot
R13368 T19487 T19488 compound /,dot
R13369 T19488 T19483 appos dot,antibodies
R13370 T19489 T19483 punct ),antibodies
R13371 T19490 T19483 punct :,antibodies
R13372 T19491 T19492 compound anti-NFκB,p50
R13373 T19492 T19506 nmod p50,p52
R13374 T19493 T19494 punct (,NLS
R13375 T19494 T19492 appos NLS,p50
R13376 T19495 T19494 punct ),NLS
R13377 T19496 T19499 amod sc-114,p65
R13378 T19497 T19499 punct ",",p65
R13379 T19498 T19499 amod anti-NFκB,p65
R13380 T19499 T19492 appos p65,p50
R13381 T19500 T19499 punct (,p65
R13382 T19501 T19499 appos A,p65
R13383 T19502 T19499 punct ),p65
R13384 T19503 T19506 nmod sc-109,p52
R13385 T19504 T19503 punct ",",sc-109
R13386 T19505 T19506 amod anti-NFκB,p52
R13387 T19506 T19483 appos p52,antibodies
R13388 T19507 T19506 punct (,p52
R13389 T19508 T19506 appos 447,p52
R13390 T19509 T19506 punct ),p52
R13391 T19510 T19506 conj sc-848,p52
R13392 T19511 T19510 punct ",",sc-848
R13393 T19512 T19516 det a,antiserum
R13394 T19513 T19516 nmod rabbit,antiserum
R13395 T19514 T19516 nmod polyclonal,antiserum
R13396 T19515 T19516 amod IgG1,antiserum
R13397 T19516 T19510 conj antiserum,sc-848
R13398 T19517 T19516 punct ",",antiserum
R13399 T19518 T19516 conj anti-c-rel,antiserum
R13400 T19519 T19518 punct (,anti-c-rel
R13401 T19520 T19518 appos N466,anti-c-rel
R13402 T19521 T19518 punct ),anti-c-rel
R13403 T19522 T19516 conj sc-272,antiserum
R13404 T19523 T19522 punct ",",sc-272
R13405 T19524 T19522 conj anti-NFATc2,sc-272
R13406 T19525 T19524 punct (,anti-NFATc2
R13407 T19526 T19524 appos M-20,anti-NFATc2
R13408 T19527 T19524 punct ),anti-NFATc2
R13409 T19528 T19540 nmod sc-1151,antisera
R13410 T19529 T19528 punct ",",sc-1151
R13411 T19530 T19528 conj anti-NFATc1,sc-1151
R13412 T19531 T19530 punct (,anti-NFATc1
R13413 T19532 T19530 appos K-18,anti-NFATc1
R13414 T19533 T19530 punct ),anti-NFATc1
R13415 T19534 T19528 conj sc-1149,sc-1151
R13416 T19535 T19534 punct ",",sc-1149
R13417 T19536 T19540 nummod two,antisera
R13418 T19537 T19540 nmod goat,antisera
R13419 T19538 T19540 nmod polyclonal,antisera
R13420 T19539 T19540 amod IgG1,antisera
R13421 T19540 T19522 dep antisera,sc-272
R13422 T19541 T19483 punct ;,antibodies
R13423 T19542 T19543 amod secondary,antibodies
R13424 T19543 T19483 appos antibodies,antibodies
R13425 T19544 T19548 punct (,dot
R13426 T19545 T19546 nummod 0.8,μg
R13427 T19546 T19548 compound μg,dot
R13428 T19547 T19548 compound /,dot
R13429 T19548 T19543 appos dot,antibodies
R13430 T19549 T19548 punct ),dot
R13431 T19550 T19543 punct :,antibodies
R13432 T19551 T19552 compound goat,anti-rabbit
R13433 T19552 T19555 compound anti-rabbit,sc-2007
R13434 T19553 T19554 compound IgG,AP-conjugate
R13435 T19554 T19555 compound AP-conjugate,sc-2007
R13436 T19555 T19543 appos sc-2007,antibodies
R13437 T19556 T19555 punct ",",sc-2007
R13438 T19557 T19555 cc and,sc-2007
R13439 T19558 T19559 amod donkey,anti-goat
R13440 T19559 T19562 amod anti-goat,sc-2022
R13441 T19560 T19561 compound IgG,AP-conjugate
R13442 T19561 T19562 compound AP-conjugate,sc-2022
R13443 T19562 T19555 conj sc-2022,sc-2007
R13444 T19563 T19478 punct .,extract
R13445 T19564 T19566 det The,proteins
R13446 T19565 T19566 amod nuclear,proteins
R13447 T19566 T19568 nsubjpass proteins,blotted
R13448 T19567 T19568 auxpass were,blotted
R13449 T19568 T19568 ROOT blotted,blotted
R13450 T19569 T19568 prep onto,blotted
R13451 T19570 T19572 det a,membrane
R13452 T19571 T19572 amod nitrocellulose,membrane
R13453 T19572 T19569 pobj membrane,onto
R13454 T19573 T19574 punct (,Bio-Rad
R13455 T19574 T19572 appos Bio-Rad,membrane
R13456 T19575 T19568 punct ),blotted
R13457 T19576 T19568 punct .,blotted
R13458 T19577 T19594 prep After,incubated
R13459 T19578 T19577 pcomp blocking,After
R13460 T19579 T19578 prep with,blocking
R13461 T19580 T19581 nummod PBS/3,%
R13462 T19581 T19584 nmod %,Fluka
R13463 T19582 T19584 nmod BSA,Fluka
R13464 T19583 T19584 punct (,Fluka
R13465 T19584 T19579 pobj Fluka,with
R13466 T19585 T19584 punct ",",Fluka
R13467 T19586 T19584 conj Deisenhofen,Fluka
R13468 T19587 T19586 punct ",",Deisenhofen
R13469 T19588 T19586 conj Germany,Deisenhofen
R13470 T19589 T19584 punct ),Fluka
R13471 T19590 T19594 punct ",",incubated
R13472 T19591 T19592 det the,blot
R13473 T19592 T19594 nsubjpass blot,incubated
R13474 T19593 T19594 auxpass was,incubated
R13475 T19594 T19594 ROOT incubated,incubated
R13476 T19595 T19594 advmod overnight,incubated
R13477 T19596 T19594 prep with,incubated
R13478 T19597 T19599 det the,antibodies
R13479 T19598 T19599 amod primary,antibodies
R13480 T19599 T19596 pobj antibodies,with
R13481 T19600 T19599 acl diluted,antibodies
R13482 T19601 T19600 npadvmod 1:2000,diluted
R13483 T19602 T19601 prep in,1:2000
R13484 T19603 T19604 nummod PBS/1,%
R13485 T19604 T19602 pobj %,in
R13486 T19605 T19600 npadvmod BSA,diluted
R13487 T19606 T19607 punct ",",washed
R13488 T19607 T19594 dep washed,incubated
R13489 T19608 T19607 advmod again,washed
R13490 T19609 T19607 cc and,washed
R13491 T19610 T19607 conj incubated,washed
R13492 T19611 T19610 prep with,incubated
R13493 T19612 T19616 det the,antibody
R13494 T19613 T19616 amod alkaline,antibody
R13495 T19614 T19616 amod phosphatase-conjugated,antibody
R13496 T19615 T19616 amod secondary,antibody
R13497 T19616 T19611 pobj antibody,with
R13498 T19617 T19616 punct (,antibody
R13499 T19618 T19616 appos 1:500,antibody
R13500 T19619 T19618 prep in,1:500
R13501 T19620 T19621 nummod PBS/1,%
R13502 T19621 T19622 compound %,BSA
R13503 T19622 T19619 pobj BSA,in
R13504 T19623 T19616 punct ),antibody
R13505 T19624 T19594 punct .,incubated
R13506 T19625 T19626 det The,blot
R13507 T19626 T19628 nsubjpass blot,developed
R13508 T19627 T19628 auxpass was,developed
R13509 T19628 T19628 ROOT developed,developed
R13510 T19629 T19628 advcl using,developed
R13511 T19630 T19632 det the,®
R13512 T19631 T19632 compound Vectastain,®
R13513 T19632 T19629 dobj ®,using
R13514 T19633 T19629 conj staining,using
R13515 T19634 T19633 dobj kit,staining
R13516 T19635 T19634 punct (,kit
R13517 T19636 T19638 compound Vector,Inc.
R13518 T19637 T19638 compound Laboratories,Inc.
R13519 T19638 T19634 appos Inc.,kit
R13520 T19639 T19628 punct .,developed
R13704 T20005 T20011 nsubjpass measurements,harvested
R13705 T20006 T20011 nsubjpass Supernatants,harvested
R13706 T20007 T20006 prep of,Supernatants
R13707 T20008 T20009 compound cell,cultures
R13708 T20009 T20007 pobj cultures,of
R13709 T20010 T20011 auxpass were,harvested
R13710 T20011 T20011 ROOT harvested,harvested
R13711 T20012 T20033 npadvmod 3,stored
R13712 T20013 T20012 punct ",",3
R13713 T20014 T20012 conj 6,3
R13714 T20015 T20014 punct ",",6
R13715 T20016 T20012 appos 9,3
R13716 T20017 T20033 punct ",",stored
R13717 T20018 T20029 nummod 12,hours
R13718 T20019 T20020 punct ",",18
R13719 T20020 T20022 nummod 18,24
R13720 T20021 T20022 punct ",",24
R13721 T20022 T20018 appos 24,12
R13722 T20023 T20024 punct ",",30
R13723 T20024 T20022 prep 30,24
R13724 T20025 T20024 punct ",",30
R13725 T20026 T20024 conj 36,30
R13726 T20027 T20026 cc and,36
R13727 T20028 T20026 conj 48,36
R13728 T20029 T20030 npadvmod hours,after
R13729 T20030 T20033 prep after,stored
R13730 T20031 T20030 pobj stimulation,after
R13731 T20032 T20030 cc and,after
R13732 T20033 T20011 oprd stored,harvested
R13733 T20034 T20033 prep at,stored
R13734 T20035 T20037 nummod -80,C
R13735 T20036 T20037 nummod °,C
R13736 T20037 T20034 pobj C,at
R13737 T20038 T20039 mark until,used
R13738 T20039 T20033 advcl used,stored
R13739 T20040 T20039 cc and,used
R13740 T20041 T20039 conj thawed,used
R13741 T20042 T20043 advmod only,once
R13742 T20043 T20041 advmod once,thawed
R13743 T20044 T20033 punct .,stored
R13744 T20045 T20053 nsubjpass IFNγ,determined
R13745 T20046 T20045 punct ",",IFNγ
R13746 T20047 T20045 conj TNFα,IFNγ
R13747 T20048 T20047 punct ",",TNFα
R13748 T20049 T20047 conj IL-1,TNFα
R13749 T20050 T20049 cc and,IL-1
R13750 T20051 T20049 conj IL-6,IL-1
R13751 T20052 T20053 auxpass were,determined
R13752 T20053 T20053 ROOT determined,determined
R13753 T20054 T20053 advcl using,determined
R13754 T20055 T20056 det an,ELISA
R13755 T20056 T20054 dobj ELISA,using
R13756 T20057 T20056 prep from,ELISA
R13757 T20058 T20059 compound Bender,Systems
R13758 T20059 T20057 pobj Systems,from
R13759 T20060 T20059 punct (,Systems
R13760 T20061 T20059 npadvmod Vienna,Systems
R13761 T20062 T20061 punct ",",Vienna
R13762 T20063 T20061 appos Austria,Vienna
R13763 T20064 T20061 punct ),Vienna
R13764 T20065 T20061 punct ",",Vienna
R13765 T20066 T20061 conj IL-1RA,Vienna
R13766 T20067 T20066 cc and,IL-1RA
R13767 T20068 T20066 conj IL-8,IL-1RA
R13768 T20069 T20054 prep by,using
R13769 T20070 T20071 det an,ELISA
R13770 T20071 T20069 pobj ELISA,by
R13771 T20072 T20071 prep from,ELISA
R13772 T20073 T20074 compound R&D,Systems
R13773 T20074 T20072 pobj Systems,from
R13774 T20075 T20074 punct (,Systems
R13775 T20076 T20071 conj Wiesbaden-Nordenstadt,ELISA
R13776 T20077 T20076 punct ",",Wiesbaden-Nordenstadt
R13777 T20078 T20076 conj Germany,Wiesbaden-Nordenstadt
R13778 T20079 T20076 punct ),Wiesbaden-Nordenstadt
R13779 T20080 T20053 punct ",",determined
R13780 T20081 T20053 cc and,determined
R13781 T20082 T20084 nsubj TGFβ,using
R13782 T20083 T20082 nummod 1,TGFβ
R13783 T20084 T20053 conj using,determined
R13784 T20085 T20086 det an,ELISA
R13785 T20086 T20084 dobj ELISA,using
R13786 T20087 T20089 mark as,described
R13787 T20088 T20089 advmod previously,described
R13788 T20089 T20084 advcl described,using
R13789 T20090 T20092 nmod [,]
R13790 T20091 T20092 nummod 57,]
R13791 T20092 T20089 dobj ],described
R13792 T20093 T20084 punct .,using
R13793 T20094 T20106 nsubjpass Quantification,done
R13794 T20095 T20094 prep of,Quantification
R13795 T20096 T20095 pobj IL-2,of
R13796 T20097 T20094 prep as,Quantification
R13797 T20098 T20099 det an,indicator
R13798 T20099 T20097 pobj indicator,as
R13799 T20100 T20099 prep for,indicator
R13800 T20101 T20102 det the,bioactivity
R13801 T20102 T20100 pobj bioactivity,for
R13802 T20103 T20102 prep of,bioactivity
R13803 T20104 T20103 pobj IL-1,of
R13804 T20105 T20106 auxpass was,done
R13805 T20106 T20106 ROOT done,done
R13806 T20107 T20109 mark as,described
R13807 T20108 T20109 advmod previously,described
R13808 T20109 T20106 advcl described,done
R13809 T20110 T20112 nmod [,]
R13810 T20111 T20112 nummod 46,]
R13811 T20112 T20109 dobj ],described
R13812 T20113 T20106 punct .,done
R13813 T20114 T20116 nsubjpass Samples,diluted
R13814 T20115 T20116 auxpass were,diluted
R13815 T20116 T20116 ROOT diluted,diluted
R13816 T20117 T20116 prep in,diluted
R13817 T20118 T20120 det the,buffer
R13818 T20119 T20120 amod same,buffer
R13819 T20120 T20117 pobj buffer,in
R13820 T20121 T20120 prep as,buffer
R13821 T20122 T20121 pobj that,as
R13822 T20123 T20122 acl used,that
R13823 T20124 T20123 prep for,used
R13824 T20125 T20126 det the,standards
R13825 T20126 T20124 pobj standards,for
R13826 T20127 T20116 punct .,diluted
R13827 T20128 T20129 det All,cytokines
R13828 T20129 T20131 nsubjpass cytokines,quantified
R13829 T20130 T20131 auxpass were,quantified
R13830 T20131 T20131 ROOT quantified,quantified
R13831 T20132 T20131 advcl using,quantified
R13832 T20133 T20136 det an,reader
R13833 T20134 T20136 nmod ELISA,reader
R13834 T20135 T20136 compound plate,reader
R13835 T20136 T20132 dobj reader,using
R13836 T20137 T20136 punct (,reader
R13837 T20138 T20139 compound Anthos,Labotec
R13838 T20139 T20136 appos Labotec,reader
R13839 T20140 T20139 punct ",",Labotec
R13840 T20141 T20139 conj Salzburg,Labotec
R13841 T20142 T20141 punct ",",Salzburg
R13842 T20143 T20141 conj Austria,Salzburg
R13843 T20144 T20143 cc or,Austria
R13844 T20145 T20146 compound Microplate,Reader
R13845 T20146 T20143 conj Reader,Austria
R13846 T20147 T20146 punct ",",Reader
R13847 T20148 T20146 conj BioRad,Reader
R13848 T20149 T20136 punct ),reader
R13849 T20150 T20131 punct .,quantified
R13850 T20151 T20152 compound Cytokine,amounts
R13851 T20152 T20153 nsubj amounts,were
R13852 T20153 T20153 ROOT were,were
R13853 T20154 T20153 dep all,were
R13854 T20155 T20153 prep within,were
R13855 T20156 T20157 det the,range
R13856 T20157 T20155 pobj range,within
R13857 T20158 T20157 prep of,range
R13858 T20159 T20161 det the,curve
R13859 T20160 T20161 amod standard,curve
R13860 T20161 T20158 pobj curve,of
R13861 T20162 T20153 punct .,were
R13862 T20163 T20164 advmod Only,stimulation
R13863 T20164 T20167 nsubj stimulation,observed
R13864 T20165 T20164 prep of,stimulation
R13865 T20166 T20165 pobj PBMC,of
R13866 T20167 T20167 ROOT observed,observed
R13867 T20168 T20167 prep in,observed
R13868 T20169 T20168 pobj donors,in
R13869 T20170 T20169 acl showing,donors
R13870 T20171 T20174 det no,release
R13871 T20172 T20174 amod spontaneous,release
R13872 T20173 T20174 compound cytokine,release
R13873 T20174 T20170 dobj release,showing
R13874 T20175 T20176 auxpass was,included
R13875 T20176 T20167 ccomp included,observed
R13876 T20177 T20176 prep in,included
R13877 T20178 T20179 det the,statistics
R13878 T20179 T20177 pobj statistics,in
R13879 T20180 T20167 punct .,observed
R14153 T20612 T20631 nsubjpass Inhibition,incubated
R14154 T20613 T20612 prep of,Inhibition
R14155 T20614 T20615 compound transcription,factors
R14156 T20615 T20613 pobj factors,of
R14157 T20616 T20617 aux To,inhibit
R14158 T20617 T20631 advcl inhibit,incubated
R14159 T20618 T20617 dobj translocation,inhibit
R14160 T20619 T20618 cc and,translocation
R14161 T20620 T20618 conj binding,translocation
R14162 T20621 T20620 prep of,binding
R14163 T20622 T20623 compound transcription,factors
R14164 T20623 T20621 pobj factors,of
R14165 T20624 T20617 prep to,inhibit
R14166 T20625 T20627 det the,promoter
R14167 T20626 T20627 compound IL-1RA,promoter
R14168 T20627 T20624 pobj promoter,to
R14169 T20628 T20631 punct ",",incubated
R14170 T20629 T20631 nsubjpass PBMC,incubated
R14171 T20630 T20631 auxpass were,incubated
R14172 T20631 T20631 ROOT incubated,incubated
R14173 T20632 T20631 prep for,incubated
R14174 T20633 T20634 nummod 1,h
R14175 T20634 T20632 pobj h,for
R14176 T20635 T20634 prep with,h
R14177 T20636 T20635 pobj each,with
R14178 T20637 T20636 prep of,each
R14179 T20638 T20640 det the,substances
R14180 T20639 T20640 amod following,substances
R14181 T20640 T20637 pobj substances,of
R14182 T20641 T20644 punct (,based
R14183 T20642 T20644 nsubjpass concentrations,based
R14184 T20643 T20644 auxpass were,based
R14185 T20644 T20631 conj based,incubated
R14186 T20645 T20644 prep upon,based
R14187 T20646 T20647 nummod 50,%
R14188 T20647 T20648 compound %,inhibition
R14189 T20648 T20645 pobj inhibition,upon
R14190 T20649 T20644 punct ),based
R14191 T20650 T20654 punct :,ml
R14192 T20651 T20652 nummod 50,μg
R14193 T20652 T20654 compound μg,ml
R14194 T20653 T20654 compound /,ml
R14195 T20654 T20654 ROOT ml,ml
R14196 T20655 T20654 prep of,ml
R14197 T20656 T20655 pobj CAPE,of
R14198 T20657 T20658 punct (,caffeic-3
R14199 T20658 T20654 dep caffeic-3,ml
R14200 T20659 T20661 punct ",4",dihydroxycinnamic-acid-phenyl-ester
R14201 T20660 T20661 punct -,dihydroxycinnamic-acid-phenyl-ester
R14202 T20661 T20658 appos dihydroxycinnamic-acid-phenyl-ester,caffeic-3
R14203 T20662 T20661 punct ),dihydroxycinnamic-acid-phenyl-ester
R14204 T20663 T20658 punct ",",caffeic-3
R14205 T20664 T20665 punct (,Biomol
R14206 T20665 T20658 appos Biomol,caffeic-3
R14207 T20666 T20665 punct ",",Biomol
R14208 T20667 T20665 npadvmod Hamburg,Biomol
R14209 T20668 T20667 punct ",",Hamburg
R14210 T20669 T20667 appos Germany,Hamburg
R14211 T20670 T20667 punct ),Hamburg
R14212 T20671 T20665 punct ;,Biomol
R14213 T20672 T20674 nummod 400,cyclosporin
R14214 T20673 T20674 compound ng/ml,cyclosporin
R14215 T20674 T20675 compound cyclosporin,A
R14216 T20675 T20678 det A,Biosciences
R14217 T20676 T20678 punct (,Biosciences
R14218 T20677 T20678 compound Calbiochem-Merck,Biosciences
R14219 T20678 T20658 appos Biosciences,caffeic-3
R14220 T20679 T20678 punct ",",Biosciences
R14221 T20680 T20681 compound Bad,Soden
R14222 T20681 T20678 conj Soden,Biosciences
R14223 T20682 T20681 punct ",",Soden
R14224 T20683 T20681 conj Germany,Soden
R14225 T20684 T20681 punct ),Soden
R14226 T20685 T20678 punct ;,Biosciences
R14227 T20686 T20687 nummod 10,μg
R14228 T20687 T20690 nmod μg,cycloheximide
R14229 T20688 T20690 nmod /,cycloheximide
R14230 T20689 T20690 compound ml,cycloheximide
R14231 T20690 T20678 conj cycloheximide,Biosciences
R14232 T20691 T20694 punct (,Biotechnology
R14233 T20692 T20693 compound Santa,Cruz
R14234 T20693 T20694 compound Cruz,Biotechnology
R14235 T20694 T20690 appos Biotechnology,cycloheximide
R14236 T20695 T20690 punct ),cycloheximide
R14237 T20696 T20699 advmod prior,with
R14238 T20697 T20696 prep to,prior
R14239 T20698 T20697 pobj incubation,to
R14240 T20699 T20658 prep with,caffeic-3
R14241 T20700 T20699 pobj GP,with
R14242 T20701 T20658 punct .,caffeic-3
R14243 T20702 T20703 compound Band,shifts
R14244 T20703 T20705 nsubjpass shifts,determined
R14245 T20704 T20705 auxpass were,determined
R14246 T20705 T20705 ROOT determined,determined
R14247 T20706 T20705 prep after,determined
R14248 T20707 T20709 nummod 1,incubation
R14249 T20708 T20709 compound h,incubation
R14250 T20709 T20706 pobj incubation,after
R14251 T20710 T20709 prep with,incubation
R14252 T20711 T20710 pobj GP,with
R14253 T20712 T20713 mark as,described
R14254 T20713 T20709 advcl described,incubation
R14255 T20714 T20713 advmod before,described
R14256 T20715 T20709 punct ",",incubation
R14257 T20716 T20709 prep including,incubation
R14258 T20717 T20718 amod mutated,oligos
R14259 T20718 T20716 pobj oligos,including
R14260 T20719 T20718 prep for,oligos
R14261 T20720 T20719 pobj NFIL-6,for
R14262 T20721 T20731 punct (,GGG
R14263 T20722 T20727 compound TTA,GCG
R14264 T20723 T20727 compound CAA,GCG
R14265 T20724 T20727 compound CAG,GCG
R14266 T20725 T20727 compound TGG,GCG
R14267 T20726 T20727 compound ATT,GCG
R14268 T20727 T20731 compound GCG,GGG
R14269 T20728 T20729 compound ACA,CTT
R14270 T20729 T20731 compound CTT,GGG
R14271 T20730 T20731 compound AGT,GGG
R14272 T20731 T20720 appos GGG,NFIL-6
R14273 T20732 T20731 punct ),GGG
R14274 T20733 T20720 cc and,NFIL-6
R14275 T20734 T20745 compound NFATP2/3,TCT
R14276 T20735 T20740 punct (,GTA
R14277 T20736 T20740 compound GGC,GTA
R14278 T20737 T20740 compound GCA,GTA
R14279 T20738 T20740 compound GAA,GTA
R14280 T20739 T20740 compound AAG,GTA
R14281 T20740 T20745 compound GTA,TCT
R14282 T20741 T20745 compound AAA,TCT
R14283 T20742 T20745 compound TAT,TCT
R14284 T20743 T20745 compound TTA,TCT
R14285 T20744 T20745 compound CTA,TCT
R14286 T20745 T20720 conj TCT,NFIL-6
R14287 T20746 T20718 punct ),oligos
R14288 T20747 T20748 amod binding,sites
R14289 T20748 T20716 pobj sites,including
R14290 T20749 T20748 prep within,sites
R14291 T20750 T20752 det the,promoter
R14292 T20751 T20752 compound IL-1RA,promoter
R14293 T20752 T20749 pobj promoter,within
R14294 T20753 T20705 punct .,determined
R14295 T20754 T20755 compound PBMC,RNA
R14296 T20755 T20756 nsubj RNA,was
R14297 T20756 T20756 ROOT was,was
R14298 T20757 T20756 acomp isolated,was
R14299 T20758 T20757 prep with,isolated
R14300 T20759 T20761 det an,kit
R14301 T20760 T20761 compound RNeasy,kit
R14302 T20761 T20758 pobj kit,with
R14303 T20762 T20761 punct (,kit
R14304 T20763 T20761 appos Qiagen,kit
R14305 T20764 T20763 punct ",",Qiagen
R14306 T20765 T20763 appos Germany,Qiagen
R14307 T20766 T20765 punct ),Germany
R14308 T20767 T20761 cc and,kit
R14309 T20768 T20769 compound messenger,RNA
R14310 T20769 T20771 nsubjpass RNA,transcribed
R14311 T20770 T20771 auxpass was,transcribed
R14312 T20771 T20756 conj transcribed,was
R14313 T20772 T20771 prep into,transcribed
R14314 T20773 T20772 pobj cDNA,into
R14315 T20774 T20771 prep with,transcribed
R14316 T20775 T20778 compound Reactin,Strand
R14317 T20776 T20778 compound Ready,Strand
R14318 T20777 T20778 compound First,Strand
R14319 T20778 T20779 compound Strand,kit
R14320 T20779 T20774 pobj kit,with
R14321 T20780 T20779 punct (,kit
R14322 T20781 T20779 appos Biomol,kit
R14323 T20782 T20779 punct ),kit
R14324 T20783 T20771 cc and,transcribed
R14325 T20784 T20771 conj analysed,transcribed
R14326 T20785 T20784 prep for,analysed
R14327 T20786 T20785 pobj IL-1RA,for
R14328 T20787 T20786 cc and,IL-1RA
R14329 T20788 T20786 conj GAPDH,IL-1RA
R14330 T20789 T20785 pobj transcripts,for
R14331 T20790 T20789 prep by,transcripts
R14332 T20791 T20790 pobj PCR,by
R14333 T20792 T20784 prep with,analysed
R14334 T20793 T20794 det a,HotStart
R14335 T20794 T20799 nmod HotStart,mastermix
R14336 T20795 T20799 punct """",mastermix
R14337 T20796 T20799 amod sweet,mastermix
R14338 T20797 T20799 punct """",mastermix
R14339 T20798 T20799 compound PCR,mastermix
R14340 T20799 T20856 nsubj mastermix,following
R14341 T20800 T20799 punct (,mastermix
R14342 T20801 T20799 appos Biomol,mastermix
R14343 T20802 T20799 punct ),mastermix
R14344 T20803 T20806 punct (,C
R14345 T20804 T20805 nummod 95,°
R14346 T20805 T20806 compound °,C
R14347 T20806 T20799 appos C,mastermix
R14348 T20807 T20813 punct ",",C
R14349 T20808 T20809 nummod 30,sec
R14350 T20809 T20813 npadvmod sec,C
R14351 T20810 T20813 punct ",",C
R14352 T20811 T20813 nummod 55,C
R14353 T20812 T20813 compound °,C
R14354 T20813 T20799 conj C,mastermix
R14355 T20814 T20813 punct ",",C
R14356 T20815 T20816 nummod 30,sec
R14357 T20816 T20813 appos sec,C
R14358 T20817 T20816 punct ",",sec
R14359 T20818 T20820 nummod 72,C
R14360 T20819 T20820 compound °,C
R14361 T20820 T20813 conj C,C
R14362 T20821 T20820 punct ",",C
R14363 T20822 T20823 nummod 30,sec
R14364 T20823 T20856 nsubj sec,following
R14365 T20824 T20823 punct ",",sec
R14366 T20825 T20826 nummod 25,cycles
R14367 T20826 T20823 conj cycles,sec
R14368 T20827 T20826 prep for,cycles
R14369 T20828 T20827 pobj IL-1RA,for
R14370 T20829 T20828 cc and,IL-1RA
R14371 T20830 T20831 nummod 94,°
R14372 T20831 T20832 compound °,C
R14373 T20832 T20828 conj C,IL-1RA
R14374 T20833 T20832 prep for,C
R14375 T20834 T20835 nummod 30,sec
R14376 T20835 T20833 pobj sec,for
R14377 T20836 T20835 punct ",",sec
R14378 T20837 T20838 nummod 50,°
R14379 T20838 T20839 compound °,C
R14380 T20839 T20832 conj C,C
R14381 T20840 T20823 prep for,sec
R14382 T20841 T20856 nummod 30,following
R14383 T20842 T20856 nsubj sec,following
R14384 T20843 T20842 cc and,sec
R14385 T20844 T20845 nummod 72,°
R14386 T20845 T20846 compound °,C
R14387 T20846 T20842 conj C,sec
R14388 T20847 T20846 prep for,C
R14389 T20848 T20849 nummod 45,sec
R14390 T20849 T20847 pobj sec,for
R14391 T20850 T20849 punct ",",sec
R14392 T20851 T20852 nummod 25,cycles
R14393 T20852 T20849 appos cycles,sec
R14394 T20853 T20852 prep for,cycles
R14395 T20854 T20853 pobj GAPDH,for
R14396 T20855 T20852 punct ),cycles
R14397 T20856 T20771 conj following,transcribed
R14398 T20857 T20858 nummod 18,h
R14399 T20858 T20859 compound h,incubation
R14400 T20859 T20856 pobj incubation,following
R14401 T20860 T20859 prep with,incubation
R14402 T20861 T20860 pobj GP,with
R14403 T20862 T20771 punct .,transcribed
R14404 T20863 T20864 compound IL-1RA,protein
R14405 T20864 T20866 nsubjpass protein,measured
R14406 T20865 T20866 auxpass was,measured
R14407 T20866 T20866 ROOT measured,measured
R14408 T20867 T20866 prep in,measured
R14409 T20868 T20869 det the,supernatant
R14410 T20869 T20867 pobj supernatant,in
R14411 T20870 T20866 agent by,measured
R14412 T20871 T20870 pobj ELISA,by
R14413 T20872 T20866 prep after,measured
R14414 T20873 T20875 nummod 24,incubation
R14415 T20874 T20875 compound h,incubation
R14416 T20875 T20872 pobj incubation,after
R14417 T20876 T20875 prep with,incubation
R14418 T20877 T20876 pobj GP,with
R14419 T20878 T20866 punct .,measured
R14585 T21188 T21189 compound Statistical,analysis
R14586 T21189 T21194 nsubjpass analysis,used
R14587 T21190 T21192 det The,test
R14588 T21191 T21192 compound Kolmogorow-Smirnov,test
R14589 T21192 T21194 nsubjpass test,used
R14590 T21193 T21194 auxpass was,used
R14591 T21194 T21194 ROOT used,used
R14592 T21195 T21196 aux to,evaluate
R14593 T21196 T21194 xcomp evaluate,used
R14594 T21197 T21196 punct ",",evaluate
R14595 T21198 T21209 mark whether,distributed
R14596 T21199 T21200 compound cytokine,amounts
R14597 T21200 T21206 nmod amounts,factors
R14598 T21201 T21200 cc and,amounts
R14599 T21202 T21203 amod binding,activities
R14600 T21203 T21200 conj activities,amounts
R14601 T21204 T21205 aux to,transcription
R14602 T21205 T21206 compound transcription,factors
R14603 T21206 T21209 nsubjpass factors,distributed
R14604 T21207 T21209 auxpass were,distributed
R14605 T21208 T21209 advmod normally,distributed
R14606 T21209 T21194 advcl distributed,used
R14607 T21210 T21194 punct .,used
R14608 T21211 T21212 compound Correlation,coefficients
R14609 T21212 T21217 nsubjpass coefficients,analyzed
R14610 T21213 T21212 cc and,coefficients
R14611 T21214 T21215 amod corresponding,significances
R14612 T21215 T21212 conj significances,coefficients
R14613 T21216 T21217 auxpass were,analyzed
R14614 T21217 T21217 ROOT analyzed,analyzed
R14615 T21218 T21217 agent by,analyzed
R14616 T21219 T21221 det the,test
R14617 T21220 T21221 compound Pearson,test
R14618 T21221 T21218 pobj test,by
R14619 T21222 T21221 punct (,test
R14620 T21223 T21224 amod normal,distribution
R14621 T21224 T21221 appos distribution,test
R14622 T21225 T21221 punct ),test
R14623 T21226 T21221 cc or,test
R14624 T21227 T21229 det the,test
R14625 T21228 T21229 compound Spearman,test
R14626 T21229 T21221 conj test,test
R14627 T21230 T21229 punct (,test
R14628 T21231 T21232 amod non-normal,distribution
R14629 T21232 T21229 appos distribution,test
R14630 T21233 T21229 punct ),test
R14631 T21234 T21217 punct .,analyzed
R14632 T21235 T21236 aux To,compare
R14633 T21236 T21256 advcl compare,calculated
R14634 T21237 T21238 amod experimental,data
R14635 T21238 T21236 dobj data,compare
R14636 T21239 T21236 prep to,compare
R14637 T21240 T21242 det a,value
R14638 T21241 T21242 amod theoretical,value
R14639 T21242 T21239 pobj value,to
R14640 T21243 T21249 punct (,vs
R14641 T21244 T21249 prep for,vs
R14642 T21245 T21244 pobj example,for
R14643 T21246 T21248 compound GP,TSST-1
R14644 T21247 T21248 compound +,TSST-1
R14645 T21248 T21249 nsubj TSST-1,vs
R14646 T21249 T21242 parataxis vs,value
R14647 T21250 T21252 nmod GP/TSST,calc
R14648 T21251 T21252 nummod -1,calc
R14649 T21252 T21249 dobj calc,vs
R14650 T21253 T21236 punct .,compare
R14651 T21254 T21256 punct ),calculated
R14652 T21255 T21256 nsubj we,calculated
R14653 T21256 T21256 ROOT calculated,calculated
R14654 T21257 T21258 det the,sum
R14655 T21258 T21256 dobj sum,calculated
R14656 T21259 T21258 prep of,sum
R14657 T21260 T21262 det the,effects
R14658 T21261 T21262 amod individual,effects
R14659 T21262 T21259 pobj effects,of
R14660 T21263 T21256 cc and,calculated
R14661 T21264 T21256 conj added,calculated
R14662 T21265 T21266 det these,values
R14663 T21266 T21264 dobj values,added
R14664 T21267 T21264 prep into,added
R14665 T21268 T21269 compound Fig.,1B
R14666 T21269 T21267 pobj 1B,into
R14667 T21270 T21269 punct ",",1B
R14668 T21271 T21269 appos 3,1B
R14669 T21272 T21271 punct ",",3
R14670 T21273 T21269 appos 4,1B
R14671 T21274 T21269 punct ",",1B
R14672 T21275 T21269 appos 5,1B
R14673 T21276 T21269 punct ",",1B
R14674 T21277 T21269 cc and,1B
R14675 T21278 T21269 conj 6,1B
R14676 T21279 T21256 punct .,calculated
R14677 T21280 T21280 ROOT Significances,Significances
R14678 T21281 T21280 prep of,Significances
R14679 T21282 T21281 pobj differences,of
R14680 T21283 T21282 prep between,differences
R14681 T21284 T21285 amod experimental,data
R14682 T21285 T21283 pobj data,between
R14683 T21286 T21282 punct (,differences
R14684 T21287 T21282 prep for,differences
R14685 T21288 T21287 pobj example,for
R14686 T21289 T21290 nsubj GP,vs
R14687 T21290 T21315 ccomp vs,analysed
R14688 T21291 T21290 dobj LPS,vs
R14689 T21292 T21290 punct ),vs
R14690 T21293 T21295 advmod as,as
R14691 T21294 T21295 advmod well,as
R14692 T21295 T21290 cc as,vs
R14693 T21296 T21295 prep between,as
R14694 T21297 T21298 amod experimental,data
R14695 T21298 T21296 pobj data,between
R14696 T21299 T21298 cc and,data
R14697 T21300 T21301 amod theoretical,values
R14698 T21301 T21298 conj values,data
R14699 T21302 T21308 punct (,vs
R14700 T21303 T21308 prep for,vs
R14701 T21304 T21303 pobj example,for
R14702 T21305 T21307 compound GP,TSST-1
R14703 T21306 T21307 compound +,TSST-1
R14704 T21307 T21308 nsubj TSST-1,vs
R14705 T21308 T21290 parataxis vs,vs
R14706 T21309 T21311 nmod GP/TSST,calc
R14707 T21310 T21311 nummod -1,calc
R14708 T21311 T21308 dobj calc,vs
R14709 T21312 T21308 punct .,vs
R14710 T21313 T21315 punct ),analysed
R14711 T21314 T21315 auxpass were,analysed
R14712 T21315 T21315 ROOT analysed,analysed
R14713 T21316 T21315 advcl using,analysed
R14714 T21317 T21318 det the,Student
R14715 T21318 T21320 poss Student,t-test
R14716 T21319 T21318 case 's,Student
R14717 T21320 T21316 dobj t-test,using
R14718 T21321 T21320 punct (,t-test
R14719 T21322 T21323 amod normal,distribution
R14720 T21323 T21320 appos distribution,t-test
R14721 T21324 T21320 punct ),t-test
R14722 T21325 T21320 cc or,t-test
R14723 T21326 T21329 det the,test
R14724 T21327 T21328 compound Wilcoxon,Signed-Rank
R14725 T21328 T21329 compound Signed-Rank,test
R14726 T21329 T21320 conj test,t-test
R14727 T21330 T21332 punct (,distribution
R14728 T21331 T21332 amod non-normal,distribution
R14729 T21332 T21329 appos distribution,test
R14730 T21333 T21329 punct ),test
R14731 T21334 T21315 punct ",",analysed
R14732 T21335 T21336 punct (,SPSS
R14733 T21336 T21315 appos SPSS,analysed
R14734 T21337 T21336 prep for,SPSS
R14735 T21338 T21337 pobj Windows,for
R14736 T21339 T21336 punct ;,SPSS
R14737 T21340 T21342 compound SPSS,Software
R14738 T21341 T21342 compound Science,Software
R14739 T21342 T21336 npadvmod Software,SPSS
R14740 T21343 T21342 punct ",",Software
R14741 T21344 T21342 conj Erkrath,Software
R14742 T21345 T21344 punct ",",Erkrath
R14743 T21346 T21344 conj Germany,Erkrath
R14744 T21347 T21336 punct ),SPSS
R14745 T21348 T21315 punct .,analysed
R12062 T17771 T17778 nsubjpass Isolation,obtained
R12063 T17772 T17771 prep of,Isolation
R12064 T17773 T17776 amod human,coats
R12065 T17774 T17775 compound PBMC,Buffy
R12066 T17775 T17776 compound Buffy,coats
R12067 T17776 T17772 pobj coats,of
R12068 T17777 T17778 auxpass were,obtained
R12069 T17778 T17778 ROOT obtained,obtained
R12070 T17779 T17778 prep from,obtained
R12071 T17780 T17782 amod healthy,donors
R12072 T17781 T17782 compound blood,donors
R12073 T17782 T17779 pobj donors,from
R12074 T17783 T17778 prep in,obtained
R12075 T17784 T17783 pobj compliance,in
R12076 T17785 T17784 prep with,compliance
R12077 T17786 T17788 det the,declaration
R12078 T17787 T17788 compound Helsinki,declaration
R12079 T17788 T17785 pobj declaration,with
R12080 T17789 T17785 cc and,with
R12081 T17790 T17785 conj with,with
R12082 T17791 T17792 det the,approval
R12083 T17792 T17790 pobj approval,with
R12084 T17793 T17792 prep of,approval
R12085 T17794 T17796 det the,committee
R12109 T17818 T17821 compound gradient,]
R12110 T17819 T17821 compound [,]
R12111 T17820 T17821 compound "54,55",]
R12112 T17821 T17807 npadvmod ],separated
R12113 T17822 T17807 punct .,separated
R12114 T17823 T17828 prep Following,cultured
R12115 T17824 T17823 pobj isolation,Following
R12116 T17825 T17828 punct ",",cultured
R12117 T17826 T17828 nsubjpass PBMC,cultured
R12118 T17827 T17828 auxpass were,cultured
R12119 T17828 T17828 ROOT cultured,cultured
R12120 T17829 T17828 prep at,cultured
R12121 T17830 T17832 det a,concentration
R12122 T17831 T17832 amod final,concentration
R12123 T17832 T17829 pobj concentration,at
R12124 T17833 T17832 prep of,concentration
R12125 T17834 T17837 nummod 3,cells/ml
R12126 T17835 T17837 nummod ×,cells/ml
R12127 T17836 T17837 nummod 106,cells/ml
R12128 T17837 T17833 pobj cells/ml,of
R12129 T17838 T17837 punct (,cells/ml
R12130 T17839 T17837 prep for,cells/ml
R12131 T17840 T17841 compound ELISA,measurements
R12132 T17841 T17839 pobj measurements,for
R12133 T17842 T17837 punct ),cells/ml
R12134 T17843 T17832 cc or,concentration
R12135 T17844 T17845 nummod 5,×
R12136 T17845 T17876 nmod ×,containing
R12137 T17846 T17845 nummod 106/ml,×
R12138 T17847 T17848 punct (,for
R12139 T17848 T17845 prep for,×
R12140 T17849 T17850 compound gel,shifts
R12141 T17850 T17848 pobj shifts,for
R12142 T17851 T17848 punct ),for
R12143 T17852 T17845 cc or,×
R12144 T17853 T17856 nummod 1,cells/ml
R12152 T17861 T17858 punct ),for
R12153 T17862 T17856 prep in,cells/ml
R12154 T17863 T17865 nmod RPMI,medium
R12155 T17864 T17865 nummod 1640,medium
R12156 T17865 T17862 pobj medium,in
R12157 T17866 T17876 punct (,containing
R12158 T17867 T17876 dep BioWhittaker,containing
R12159 T17868 T17867 punct ",",BioWhittaker
R12160 T17869 T17867 conj Heidelberg,BioWhittaker
R12161 T17870 T17869 punct ",",Heidelberg
R12162 T17871 T17867 npadvmod Germany,BioWhittaker
R12163 T17872 T17867 punct ",",BioWhittaker
R12164 T17873 T17867 appos LPS-free,BioWhittaker
R12165 T17874 T17873 punct ),LPS-free
R12166 T17875 T17876 punct ",",containing
R12167 T17876 T17828 advcl containing,cultured
R12168 T17877 T17878 nummod 10,%
R12169 T17878 T17882 nmod %,serum
R12170 T17879 T17882 amod heat-inactivated,serum
R12171 T17880 T17881 amod fetal,calf
R12172 T17881 T17882 compound calf,serum
R12173 T17882 T17876 dobj serum,containing
R12174 T17883 T17905 punct (,streptomycin
R12175 T17884 T17905 nmod FCS,streptomycin
R12176 T17885 T17884 punct ",",FCS
R12177 T17886 T17884 conj low-LPS,FCS
R12178 T17887 T17886 punct ",",low-LPS
R12179 T17888 T17886 conj Invitrogen,low-LPS
R12180 T17889 T17888 punct ",",Invitrogen
R12181 T17890 T17888 conj Karlsruhe,Invitrogen
R13276 T19395 T19401 nsubj Immuno,determine
R13703 T20004 T20005 amod Cytokine,measurements
R11855 T17554 T17547 punct .,prepared
R11856 T17555 T17557 det The,characteristics
R11857 T17556 T17557 amod physicochemical,characteristics
R11858 T17557 T17561 nsubjpass characteristics,determined
R11859 T17558 T17557 prep of,characteristics
R11860 T17559 T17558 pobj GP,of
R11861 T17560 T17561 auxpass were,determined
R11862 T17561 T17561 ROOT determined,determined
R11863 T17562 T17563 mark as,reported
R11864 T17563 T17561 advcl reported,determined
R11865 T17564 T17567 advmod previously,]
R11866 T17565 T17567 compound [,]
R11867 T17566 T17567 compound "20,26",]
R11868 T17567 T17563 nsubj ],reported
R11869 T17568 T17561 punct .,determined
R11870 T17569 T17571 nsubjpass GP,dissolved
R11871 T17570 T17571 auxpass was,dissolved
R11872 T17571 T17571 ROOT dissolved,dissolved
R11873 T17572 T17571 prep in,dissolved
R11874 T17573 T17574 amod aqueous,media
R11875 T17574 T17572 pobj media,in

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T18512 2532-2539 Positive_regulation denotes crucial
T18511 2532-2539 Positive_regulation denotes crucial
T18510 2548-2555 Binding denotes binding
T18509 2548-2555 Binding denotes binding
T18508 2395-2403 Entity denotes promoter
T18507 2568-2581 Protein denotes NFκB proteins
T18506 2559-2563 Protein denotes NFAT
T18505 2448-2461 Protein denotes TNFα promoter
T18504 2420-2440 Protein denotes κ consensus sequence
T18503 2390-2394 Protein denotes IFNγ
T18502 2375-2382 Protein denotes P2 site
T18501 2171-2174 Protein denotes PNK
T18500 2166-2169 Protein denotes Kit
T18499 1905-1934 Protein denotes IL-8, IFNγ and IL-6 promoters
T18498 1899-1903 Protein denotes TNFα
T19230 4381-4384 Protein denotes IgG
T19229 4298-4327 Protein denotes goat polyclonal IgG1 antisera
T19228 4224-4228 Protein denotes N466
T19227 4212-4222 Protein denotes anti-c-rel
T19226 4185-4210 Protein denotes polyclonal IgG1 antiserum
T19225 4153-4157 Protein denotes NFκB
T19224 4127-4131 Protein denotes NFκB
T19223 4094-4107 Protein denotes anti-NFκB p50
T19222 3970-3978 Protein denotes NFκB p52
T19221 3717-3721 Protein denotes NFAT
T19220 3708-3712 Protein denotes NFκB
T20315 6963-6977 Protein denotes IL-1RA protein
T20314 6921-6926 Protein denotes GAPDH
T20313 6842-6848 Protein denotes IL-1RA
T20312 6713-6730 Protein denotes GAPDH transcripts
T20311 6702-6708 Protein denotes IL-1RA
T20310 6645-6652 Protein denotes Reactin
T20309 6520-6535 Protein denotes IL-1RA promoter
T20308 6486-6493 Protein denotes CTA TCT
T20307 6466-6485 Protein denotes AAG GTA AAA TAT TTA
T20306 6454-6461 Protein denotes GGC GCA
T20305 6444-6452 Protein denotes NFATP2/3
T20304 6431-6438 Protein denotes AGT GGG
T20303 6415-6430 Protein denotes ATT GCG ACA CTT
T20302 6399-6414 Protein denotes TTA CAA CAG TGG
T20301 6391-6397 Protein denotes NFIL-6
T19782 5373-5377 Protein denotes IL-1
T19781 5329-5333 Protein denotes IL-2
T19780 5259-5265 Protein denotes TGFβ 1
T19779 5187-5191 Protein denotes IL-8
T19778 5176-5182 Protein denotes IL-1RA
T19777 5101-5105 Protein denotes IL-6
T19776 5092-5096 Protein denotes IL-1
T19775 5086-5090 Protein denotes TNFα
T19256 4719-4739 Protein denotes alkaline phosphatase
T19255 4524-4527 Protein denotes Rad
T19254 4428-4431 Protein denotes IgG
T21010 7556-7559 Protein denotes Fig
T18810 3618-3625 Binding denotes binding
T18809 3537-3542 Protein denotes Oct-1
T18808 3406-3410 Protein denotes NFκB
T17952 1733-1739 Protein denotes TSST-1
T17951 1726-1728 Protein denotes GP
R12657 T18502 T18508 partOf P2 site,promoter
R12658 T18503 T18508 partOf IFNγ,promoter
R12659 T18506 T18509 themeOf NFAT,binding
R12660 T18507 T18510 themeOf NFκB proteins,binding
R12661 T18509 T18511 themeOf binding,crucial
R12662 T18510 T18512 themeOf binding,crucial
R12865 T18809 T18810 themeOf Oct-1,binding

testone

Id Subject Object Predicate Lexical cue
T20972 7751-7757 Protein denotes TSST-1
T20971 7738-7744 Protein denotes TSST-1
T20970 7466-7472 Protein denotes TSST-1
T20969 7453-7459 Protein denotes TSST-1
T20241 5908-5916 Entity denotes promoter
T20240 5861-5868 Binding denotes binding
T20239 6963-6969 Protein denotes IL-1RA
T20238 6713-6718 Protein denotes GAPDH
T20237 6702-6708 Protein denotes IL-1RA
T20236 6520-6526 Protein denotes IL-1RA
T20235 6451-6452 Protein denotes 3
T20234 6444-6450 Protein denotes NFATP2
T20233 6391-6397 Protein denotes NFIL-6
T20232 5901-5907 Protein denotes IL-1RA
T19724 5373-5377 Protein denotes IL-1
T19723 5329-5333 Protein denotes IL-2
T19722 5259-5265 Protein denotes TGFβ 1
T19721 5187-5191 Protein denotes IL-8
T19720 5176-5182 Protein denotes IL-1RA
T19719 5101-5105 Protein denotes IL-6
T19718 5092-5096 Protein denotes IL-1
T19717 5086-5090 Protein denotes TNFα
T19716 5080-5084 Protein denotes IFNγ
T19185 4271-4277 Protein denotes NFATc1
T19184 4243-4249 Protein denotes NFATc2
T19183 4217-4222 Protein denotes c-rel
T19182 4158-4161 Protein denotes p52
T19181 4132-4135 Protein denotes p65
T19180 4104-4107 Protein denotes p50
T19179 3998-4003 Protein denotes c-Rel
T19178 3975-3978 Protein denotes p52
T19177 3717-3721 Protein denotes NFAT
T18779 3618-3625 Binding denotes binding
T18778 3232-3239 Binding denotes binding
T18777 3537-3542 Protein denotes Oct-1
T18776 3431-3433 Protein denotes 50
T18775 3427-3430 Protein denotes p65
T18250 2548-2555 Binding denotes binding
T18249 2532-2539 Positive_regulation denotes crucial
T18248 2358-2367 Negative_regulation denotes Mutations
T18247 2272-2279 Binding denotes binding
T18246 2559-2563 Protein denotes NFAT
T18245 2448-2452 Protein denotes TNFα
T18244 2390-2394 Protein denotes IFNγ
T18243 1920-1924 Protein denotes IL-6
T18242 1911-1915 Protein denotes IFNγ
T18241 1905-1909 Protein denotes IL-8
T18240 1899-1903 Protein denotes TNFα
T17934 1733-1739 Protein denotes TSST-1
T17933 1485-1491 Protein denotes TSST-1
T17459 33-47 Phosphorylation denotes phosphorylated
R12462 T18246 T18250 themeOf NFAT,binding
R12463 T18250 T18249 themeOf binding,crucial
R12863 T18777 T18779 themeOf Oct-1,binding
R13881 T20241 T20232 partOf promoter,IL-1RA
R13882 T20241 T20240 themeOf promoter,binding

test3

Id Subject Object Predicate Lexical cue
T20980 7751-7757 Protein denotes TSST-1
T20979 7738-7744 Protein denotes TSST-1
T20978 7466-7472 Protein denotes TSST-1
T20977 7453-7459 Protein denotes TSST-1
T20976 7751-7757 Protein denotes TSST-1
T20975 7738-7744 Protein denotes TSST-1
T20974 7466-7472 Protein denotes TSST-1
T20973 7453-7459 Protein denotes TSST-1
T20260 6963-6969 Protein denotes IL-1RA
T20259 6719-6730 Transcription denotes transcripts
T20258 6713-6718 Protein denotes GAPDH
T20257 6702-6708 Protein denotes IL-1RA
T20256 6520-6526 Protein denotes IL-1RA
T20255 6451-6452 Protein denotes 3
T20254 6444-6450 Protein denotes NFATP2
T20253 6391-6397 Protein denotes NFIL-6
T20252 5901-5907 Protein denotes IL-1RA
T20251 5861-5868 Binding denotes binding
T20250 5835-5842 Negative_regulation denotes inhibit
T20249 6963-6969 Protein denotes IL-1RA
T20248 6713-6718 Protein denotes GAPDH
T20247 6702-6708 Protein denotes IL-1RA
T20246 6520-6526 Protein denotes IL-1RA
T20245 6451-6452 Protein denotes 3
T20244 6444-6450 Protein denotes NFATP2
T20243 6391-6397 Protein denotes NFIL-6
T20242 5901-5907 Protein denotes IL-1RA
T19742 5373-5377 Protein denotes IL-1
T19741 5329-5333 Protein denotes IL-2
T19740 5259-5265 Protein denotes TGFβ 1
T19739 5187-5191 Protein denotes IL-8
T19738 5176-5182 Protein denotes IL-1RA
T19737 5101-5105 Protein denotes IL-6
T19736 5092-5096 Protein denotes IL-1
T19735 5086-5090 Protein denotes TNFα
T19734 5080-5084 Protein denotes IFNγ
T19733 5373-5377 Protein denotes IL-1
T19732 5329-5333 Protein denotes IL-2
T19731 5259-5265 Protein denotes TGFβ 1
T19730 5187-5191 Protein denotes IL-8
T19729 5176-5182 Protein denotes IL-1RA
T19728 5101-5105 Protein denotes IL-6
T19727 5092-5096 Protein denotes IL-1
T19726 5086-5090 Protein denotes TNFα
T19725 5080-5084 Protein denotes IFNγ
T19194 4271-4277 Protein denotes NFATc1
T19193 4243-4249 Protein denotes NFATc2
T19192 4217-4222 Protein denotes c-rel
T19191 4158-4161 Protein denotes p52
T19190 4132-4135 Protein denotes p65
T19189 4104-4107 Protein denotes p50
T19188 3998-4003 Protein denotes c-Rel
T19187 3975-3978 Protein denotes p52
T19186 3717-3721 Protein denotes NFAT
T18787 3618-3625 Binding denotes binding
T18786 3537-3542 Protein denotes Oct-1
T18785 3431-3433 Protein denotes 50
T18784 3427-3430 Protein denotes p65
T18783 3232-3239 Binding denotes binding
T18782 3537-3542 Protein denotes Oct-1
T18781 3431-3433 Protein denotes 50
T18780 3427-3430 Protein denotes p65
T18260 1911-1915 Protein denotes IFNγ
T18259 1905-1909 Protein denotes IL-8
T18258 1899-1903 Protein denotes TNFα
T18257 2559-2563 Protein denotes NFAT
T18256 2448-2452 Protein denotes TNFα
T18255 2390-2394 Protein denotes IFNγ
T18254 1920-1924 Protein denotes IL-6
T18253 1911-1915 Protein denotes IFNγ
T18252 1905-1909 Protein denotes IL-8
T18251 1899-1903 Protein denotes TNFα
T17938 1733-1739 Protein denotes TSST-1
T17937 1485-1491 Protein denotes TSST-1
T17936 1733-1739 Protein denotes TSST-1
T17935 1485-1491 Protein denotes TSST-1
T18266 2559-2563 Protein denotes NFAT
T18265 2548-2555 Binding denotes binding
T18264 2532-2539 Positive_regulation denotes crucial
T18263 2448-2452 Protein denotes TNFα
T18262 2390-2394 Protein denotes IFNγ
T18261 1920-1924 Protein denotes IL-6
T17460 33-47 Phosphorylation denotes phosphorylated
R12464 T18265 T18264 themeOf binding,crucial
R12465 T18266 T18265 themeOf NFAT,binding
R12864 T18786 T18787 themeOf Oct-1,binding
R13883 T20251 T20250 themeOf binding,inhibit
R13884 T20257 T20259 themeOf IL-1RA,transcripts
R13885 T20258 T20259 themeOf GAPDH,transcripts