PMC:1472690 / 19658-21538
Annnotations
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With respect to TSST-1 or the theoretical value of GP/TSST-1 calc. vs. GP + TSST-1, we observed a pronounced anti-inflammatory action of GP, as demonstrated by an about 10–100 fold reduced ratio at 18 h and 24 h of incubation, i.e. a higher production of IL-1RA than of IL-1β (n = 3; both p \u003c 0.05; Fig. 6). A biological relevance of this result was suggested by a decrease of the IL-1 dependent release of IL-2 from murine EL-4 cells following GP + TSST-1 when compared to TSST-1 or GP/TSST-1 calc. (data not shown in detail, n = 4; p \u003c 0.07).\nFigure 6 Decreased IL-1β/IL-1RA ratio following GP + TSST-1 when compared to TSST-1. Time course (48 h) of the IL-1β/IL-1RA ratio following incubation of human PBMC with LPS, TSST-1, GP + LPS, GP/LPS calc., GP + TSST-1 or GP/TSST-1 calc. LPS and GP + LPS treatments resulted in generating more IL-1β than IL-1RA up to 24 h. When comparing LPS or GP/LPS calc. vs GP + LPS we did not find significant alterations in the IL-1β/IL-1RA ratio over 48 h. With respect to TSST-1 or GP/TSST-1 calc. vs GP + TSST-1 we observed a pronounced anti-inflammatory action of GP, as demonstrated by an about 10–100 fold reduced ratio at 18 h and 24 h, i.e. a higher production of IL-1RA than of IL-1β (* = p \u003c 0.05). 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