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pmc-enju-pas

bionlp-st-ge-2016-test-proteins

bionlp-st-ge-2016-uniprot

UBERON-AE

{"project":"UBERON-AE","denotations":[{"id":"T4718","span":{"begin":454,"end":459},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"}],"text":"Cytokine profile of human PBMC following a 48 h in vitro stimulation with LPS, TSST-1, GP, GP + LPS and GP + TSST-1\nBecause of the GP-induced band shifts to seven sites from the TNFα, IL-8, IL-6 and IFNγ promoters, it could be assumed that GP treatment of human PBMC would lead to production of TNFα, IL-8, IL-6, IFNγ and other pro-inflammatory mediators. Previous studies reported that β-1→3-D-glucans induced only a limited cytokine secretion of human blood cells [15-18]. Corroborating and extending these singular findings in terms of examining pro- (eight) as well as anti- (three) inflammatory cytokines over time (48 h) and six additional transcription factor binding sites, our comprehensive analysis revealed the following cytokine profile of human leukocytes in response to a highly purified water soluble β-1→3-D-glucan and in comparison to two pro-inflammatory mediators (LPS, TSST-1):\n\n(i) IL-1β\nThere was no IL-1β production detectable following PBMC treatment with 1 nor 100 μg GP. An insignificant up-regulation of IL-1β production for GP + LPS was observed in comparison with LPS. For GP + TSST-1 we found a significant reduction in IL-1β from 18 h – 24 h, for the latter by about 40% when compared to TSST-1 or the theoretical value of GP/TSST-1 calc. (n = 6; both p = 0.01). On the whole, GP mediated a reduction of the TSST-1-induced amount of IL-1β by about 50% (Fig. 3A).\nFigure 3 A, IL-1β was down-regulated (12 h – 24 h) following GP + TSST-1 when compared to TSST-1. Time course (48 h) of IL-1β production (pg/ml) by human PBMC incubated with medium control, 100 μg GP, 250 ng TSST-1, GP + TSST-1, 250 ng LPS and GP + LPS. At 18 h and 24 h there was a marked reduction in IL-1β production following GP + TSST-1 when compared to TSST-1 (* = p \u003c 0.05). Graphs depicting the theoretical values for GP/LPS calc. and GP/TSST-1 calc. are also shown. IL-1β levels in the supernatnant were obtained by Elisa and are shown as mean ± SEM (n = 3). B, IL-1RA was exaggerated following GP + TSST-1 (12 h – 48 h) when compared to TSST-1. Time course (48 h) of IL-1RA production (pg/ml) by human PBMC incubated with medium control, 100 μg GP, 250 ng TSST-1, GP + TSST-1, 250 ng LPS and GP + LPS. Between 18 h and 48 h a simultaneous treatment of PBMC with GP + TSST-1 led to a synergistic effect, i.e. a higher IL-1RA production when compared to an addition of the single values for TSST-1 and GP (* = p \u003c 0.05). Graphs depicting the theoretical values for GP/LPS calc. and GP/TSST-1 calc. are included. IL-1RA levels in the supernatnant were obtained by Elisa and are shown as mean ± SEM (n = 3).\n\n(ii) IL-6\nGP induced only a small amount of IL-6 and there was no significant alteration of the LPS-induced IL-6 production by GP. On the contrary, we observed a decreased IL-6 production for GP + TSST-1 when compared to TSST-1 or GP/TSST-1 calc., especially at 24 h by about 40% (n = 5; both p = 0.02), (data not shown in detail).\n\n(iii) IL-8\nWe found that GP induced a substantial IL-8 production, in comparison with medium control, especially at 24 h (n = 13; p \u003c 0.01). A combination of GP and LPS resulted in a non-significant increase of IL-8 production when compared to LPS or GP/LPS calc. There was no significant alteration in IL-8 production following GP + TSST-1 when compared to TSST-1 or GP/TSST-1 calc. (data not shown in detail).\n\n(iv) IL-1RA\nBesides IL-8, IL-1RA was the only mediator which was produced in significant quantities following GP treatment, especially at 24 h (n = 14, p \u003c 0.01). When compared to LPS stimulation or GP/LPS calc., GP + LPS did not alter the kinetic course of the IL-1RA production. However, following GP + TSST-1 we found a synergistic increase in IL-1RA production from 18 h to 48 h, when compared to TSST-1 (for instance at 24 h: n = 6; p = 0.01) or to the theoretical value of GP/TSST-1 calc. (from 18 h to 36 h). Over the time course of 48 h, GP elevated the TSST-1-induced amount of IL-1RA by approximately 200% (Fig. 3B).\nPositive correlation between GP-induced IL-8 and IL-1RA productions. Following stimulation with GP (24 h), we observed a positive correlation between IL-8 and IL-1RA. Moreover, we found this correlation to be dose-dependent, since 100 μg of GP induced larger amounts of IL-8 and IL-1RA than 1 μg GP (n = 5, p ≤ 0.002, r = 0.9 and 0.98, respectively).\n\n(v) TNFα\nWith respect to TNFα production, 100 μg of GP yielded minor, statistically not distinguishable amounts, when compared to medium control. GP + LPS did not change the TNFα production when compared to LPS supplementation or GP/LPS calc. When combined with TSST-1 or GP/TSST-1 calc., GP seemed to exert a synergistic effect on TNFα secretion after 36 h (n = 3; p \u003c 0.05), but overall an enhancement of only 10% was observed (p \u003e 0.05; data not shown in detail).\n\n(vi) IFNγ\nNo IFNγ production was detectable following treatment of PBMC with 100 μg GP. There was a minor increase in IFNγ production following GP + LPS at 36 h, when compared to LPS or GP/LPS calc., and a slight down-regulation following GP + TSST-1 at 24 h and 36 h, when compared to TSST-1 or GP/TSST-1 calc. (data not shown).\n\n(vii) IL-2, IL-12, IL-4, IL-10, TGFβ1\nThe production of IL-2, IL-12, IL-4, IL-10 and TGFβ 1 was not induced by GP. There were also no significant differences between medium control, LPS, TSST-1, GP, GP + LPS, GP/LPS calc. and GP + TSST-1 or GP/TSST-1 calc. over 48 h (data not shown)."}

GO-BP

{"project":"GO-BP","denotations":[{"id":"T4761","span":{"begin":2718,"end":2733},"obj":"http://purl.obolibrary.org/obo/GO_0032675"},{"id":"T4760","span":{"begin":2782,"end":2797},"obj":"http://purl.obolibrary.org/obo/GO_0032635"},{"id":"T4759","span":{"begin":2718,"end":2733},"obj":"http://purl.obolibrary.org/obo/GO_0032635"},{"id":"T4758","span":{"begin":5021,"end":5031},"obj":"http://purl.obolibrary.org/obo/GO_0065007"},{"id":"T4757","span":{"begin":1017,"end":1027},"obj":"http://purl.obolibrary.org/obo/GO_0065007"},{"id":"T4756","span":{"begin":646,"end":659},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T4755","span":{"begin":4672,"end":4681},"obj":"http://purl.obolibrary.org/obo/GO_0046903"},{"id":"T4754","span":{"begin":435,"end":444},"obj":"http://purl.obolibrary.org/obo/GO_0046903"},{"id":"T4753","span":{"begin":426,"end":444},"obj":"http://purl.obolibrary.org/obo/GO_0050663"},{"id":"T4770","span":{"begin":5176,"end":5194},"obj":"http://purl.obolibrary.org/obo/GO_0032663"},{"id":"T4769","span":{"begin":5176,"end":5194},"obj":"http://purl.obolibrary.org/obo/GO_0032623"},{"id":"T4768","span":{"begin":3246,"end":3261},"obj":"http://purl.obolibrary.org/obo/GO_0032637"},{"id":"T4767","span":{"begin":3154,"end":3169},"obj":"http://purl.obolibrary.org/obo/GO_0032637"},{"id":"T4766","span":{"begin":2993,"end":3008},"obj":"http://purl.obolibrary.org/obo/GO_0032637"},{"id":"T4765","span":{"begin":3246,"end":3261},"obj":"http://purl.obolibrary.org/obo/GO_0032677"},{"id":"T4764","span":{"begin":3154,"end":3169},"obj":"http://purl.obolibrary.org/obo/GO_0032677"},{"id":"T4763","span":{"begin":2993,"end":3008},"obj":"http://purl.obolibrary.org/obo/GO_0032677"},{"id":"T4762","span":{"begin":2782,"end":2797},"obj":"http://purl.obolibrary.org/obo/GO_0032675"}],"text":"Cytokine profile of human PBMC following a 48 h in vitro stimulation with LPS, TSST-1, GP, GP + LPS and GP + TSST-1\nBecause of the GP-induced band shifts to seven sites from the TNFα, IL-8, IL-6 and IFNγ promoters, it could be assumed that GP treatment of human PBMC would lead to production of TNFα, IL-8, IL-6, IFNγ and other pro-inflammatory mediators. Previous studies reported that β-1→3-D-glucans induced only a limited cytokine secretion of human blood cells [15-18]. Corroborating and extending these singular findings in terms of examining pro- (eight) as well as anti- (three) inflammatory cytokines over time (48 h) and six additional transcription factor binding sites, our comprehensive analysis revealed the following cytokine profile of human leukocytes in response to a highly purified water soluble β-1→3-D-glucan and in comparison to two pro-inflammatory mediators (LPS, TSST-1):\n\n(i) IL-1β\nThere was no IL-1β production detectable following PBMC treatment with 1 nor 100 μg GP. An insignificant up-regulation of IL-1β production for GP + LPS was observed in comparison with LPS. For GP + TSST-1 we found a significant reduction in IL-1β from 18 h – 24 h, for the latter by about 40% when compared to TSST-1 or the theoretical value of GP/TSST-1 calc. (n = 6; both p = 0.01). On the whole, GP mediated a reduction of the TSST-1-induced amount of IL-1β by about 50% (Fig. 3A).\nFigure 3 A, IL-1β was down-regulated (12 h – 24 h) following GP + TSST-1 when compared to TSST-1. Time course (48 h) of IL-1β production (pg/ml) by human PBMC incubated with medium control, 100 μg GP, 250 ng TSST-1, GP + TSST-1, 250 ng LPS and GP + LPS. At 18 h and 24 h there was a marked reduction in IL-1β production following GP + TSST-1 when compared to TSST-1 (* = p \u003c 0.05). Graphs depicting the theoretical values for GP/LPS calc. and GP/TSST-1 calc. are also shown. IL-1β levels in the supernatnant were obtained by Elisa and are shown as mean ± SEM (n = 3). B, IL-1RA was exaggerated following GP + TSST-1 (12 h – 48 h) when compared to TSST-1. Time course (48 h) of IL-1RA production (pg/ml) by human PBMC incubated with medium control, 100 μg GP, 250 ng TSST-1, GP + TSST-1, 250 ng LPS and GP + LPS. Between 18 h and 48 h a simultaneous treatment of PBMC with GP + TSST-1 led to a synergistic effect, i.e. a higher IL-1RA production when compared to an addition of the single values for TSST-1 and GP (* = p \u003c 0.05). Graphs depicting the theoretical values for GP/LPS calc. and GP/TSST-1 calc. are included. IL-1RA levels in the supernatnant were obtained by Elisa and are shown as mean ± SEM (n = 3).\n\n(ii) IL-6\nGP induced only a small amount of IL-6 and there was no significant alteration of the LPS-induced IL-6 production by GP. On the contrary, we observed a decreased IL-6 production for GP + TSST-1 when compared to TSST-1 or GP/TSST-1 calc., especially at 24 h by about 40% (n = 5; both p = 0.02), (data not shown in detail).\n\n(iii) IL-8\nWe found that GP induced a substantial IL-8 production, in comparison with medium control, especially at 24 h (n = 13; p \u003c 0.01). A combination of GP and LPS resulted in a non-significant increase of IL-8 production when compared to LPS or GP/LPS calc. There was no significant alteration in IL-8 production following GP + TSST-1 when compared to TSST-1 or GP/TSST-1 calc. (data not shown in detail).\n\n(iv) IL-1RA\nBesides IL-8, IL-1RA was the only mediator which was produced in significant quantities following GP treatment, especially at 24 h (n = 14, p \u003c 0.01). When compared to LPS stimulation or GP/LPS calc., GP + LPS did not alter the kinetic course of the IL-1RA production. However, following GP + TSST-1 we found a synergistic increase in IL-1RA production from 18 h to 48 h, when compared to TSST-1 (for instance at 24 h: n = 6; p = 0.01) or to the theoretical value of GP/TSST-1 calc. (from 18 h to 36 h). Over the time course of 48 h, GP elevated the TSST-1-induced amount of IL-1RA by approximately 200% (Fig. 3B).\nPositive correlation between GP-induced IL-8 and IL-1RA productions. Following stimulation with GP (24 h), we observed a positive correlation between IL-8 and IL-1RA. Moreover, we found this correlation to be dose-dependent, since 100 μg of GP induced larger amounts of IL-8 and IL-1RA than 1 μg GP (n = 5, p ≤ 0.002, r = 0.9 and 0.98, respectively).\n\n(v) TNFα\nWith respect to TNFα production, 100 μg of GP yielded minor, statistically not distinguishable amounts, when compared to medium control. GP + LPS did not change the TNFα production when compared to LPS supplementation or GP/LPS calc. When combined with TSST-1 or GP/TSST-1 calc., GP seemed to exert a synergistic effect on TNFα secretion after 36 h (n = 3; p \u003c 0.05), but overall an enhancement of only 10% was observed (p \u003e 0.05; data not shown in detail).\n\n(vi) IFNγ\nNo IFNγ production was detectable following treatment of PBMC with 100 μg GP. There was a minor increase in IFNγ production following GP + LPS at 36 h, when compared to LPS or GP/LPS calc., and a slight down-regulation following GP + TSST-1 at 24 h and 36 h, when compared to TSST-1 or GP/TSST-1 calc. (data not shown).\n\n(vii) IL-2, IL-12, IL-4, IL-10, TGFβ1\nThe production of IL-2, IL-12, IL-4, IL-10 and TGFβ 1 was not induced by GP. There were also no significant differences between medium control, LPS, TSST-1, GP, GP + LPS, GP/LPS calc. and GP + TSST-1 or GP/TSST-1 calc. over 48 h (data not shown)."}

GO-MF

GO-CC

{"project":"GO-CC","denotations":[{"id":"T5022","span":{"begin":460,"end":465},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Cytokine profile of human PBMC following a 48 h in vitro stimulation with LPS, TSST-1, GP, GP + LPS and GP + TSST-1\nBecause of the GP-induced band shifts to seven sites from the TNFα, IL-8, IL-6 and IFNγ promoters, it could be assumed that GP treatment of human PBMC would lead to production of TNFα, IL-8, IL-6, IFNγ and other pro-inflammatory mediators. Previous studies reported that β-1→3-D-glucans induced only a limited cytokine secretion of human blood cells [15-18]. Corroborating and extending these singular findings in terms of examining pro- (eight) as well as anti- (three) inflammatory cytokines over time (48 h) and six additional transcription factor binding sites, our comprehensive analysis revealed the following cytokine profile of human leukocytes in response to a highly purified water soluble β-1→3-D-glucan and in comparison to two pro-inflammatory mediators (LPS, TSST-1):\n\n(i) IL-1β\nThere was no IL-1β production detectable following PBMC treatment with 1 nor 100 μg GP. An insignificant up-regulation of IL-1β production for GP + LPS was observed in comparison with LPS. For GP + TSST-1 we found a significant reduction in IL-1β from 18 h – 24 h, for the latter by about 40% when compared to TSST-1 or the theoretical value of GP/TSST-1 calc. (n = 6; both p = 0.01). On the whole, GP mediated a reduction of the TSST-1-induced amount of IL-1β by about 50% (Fig. 3A).\nFigure 3 A, IL-1β was down-regulated (12 h – 24 h) following GP + TSST-1 when compared to TSST-1. Time course (48 h) of IL-1β production (pg/ml) by human PBMC incubated with medium control, 100 μg GP, 250 ng TSST-1, GP + TSST-1, 250 ng LPS and GP + LPS. At 18 h and 24 h there was a marked reduction in IL-1β production following GP + TSST-1 when compared to TSST-1 (* = p \u003c 0.05). Graphs depicting the theoretical values for GP/LPS calc. and GP/TSST-1 calc. are also shown. IL-1β levels in the supernatnant were obtained by Elisa and are shown as mean ± SEM (n = 3). B, IL-1RA was exaggerated following GP + TSST-1 (12 h – 48 h) when compared to TSST-1. Time course (48 h) of IL-1RA production (pg/ml) by human PBMC incubated with medium control, 100 μg GP, 250 ng TSST-1, GP + TSST-1, 250 ng LPS and GP + LPS. Between 18 h and 48 h a simultaneous treatment of PBMC with GP + TSST-1 led to a synergistic effect, i.e. a higher IL-1RA production when compared to an addition of the single values for TSST-1 and GP (* = p \u003c 0.05). Graphs depicting the theoretical values for GP/LPS calc. and GP/TSST-1 calc. are included. IL-1RA levels in the supernatnant were obtained by Elisa and are shown as mean ± SEM (n = 3).\n\n(ii) IL-6\nGP induced only a small amount of IL-6 and there was no significant alteration of the LPS-induced IL-6 production by GP. On the contrary, we observed a decreased IL-6 production for GP + TSST-1 when compared to TSST-1 or GP/TSST-1 calc., especially at 24 h by about 40% (n = 5; both p = 0.02), (data not shown in detail).\n\n(iii) IL-8\nWe found that GP induced a substantial IL-8 production, in comparison with medium control, especially at 24 h (n = 13; p \u003c 0.01). A combination of GP and LPS resulted in a non-significant increase of IL-8 production when compared to LPS or GP/LPS calc. There was no significant alteration in IL-8 production following GP + TSST-1 when compared to TSST-1 or GP/TSST-1 calc. (data not shown in detail).\n\n(iv) IL-1RA\nBesides IL-8, IL-1RA was the only mediator which was produced in significant quantities following GP treatment, especially at 24 h (n = 14, p \u003c 0.01). When compared to LPS stimulation or GP/LPS calc., GP + LPS did not alter the kinetic course of the IL-1RA production. However, following GP + TSST-1 we found a synergistic increase in IL-1RA production from 18 h to 48 h, when compared to TSST-1 (for instance at 24 h: n = 6; p = 0.01) or to the theoretical value of GP/TSST-1 calc. (from 18 h to 36 h). Over the time course of 48 h, GP elevated the TSST-1-induced amount of IL-1RA by approximately 200% (Fig. 3B).\nPositive correlation between GP-induced IL-8 and IL-1RA productions. Following stimulation with GP (24 h), we observed a positive correlation between IL-8 and IL-1RA. Moreover, we found this correlation to be dose-dependent, since 100 μg of GP induced larger amounts of IL-8 and IL-1RA than 1 μg GP (n = 5, p ≤ 0.002, r = 0.9 and 0.98, respectively).\n\n(v) TNFα\nWith respect to TNFα production, 100 μg of GP yielded minor, statistically not distinguishable amounts, when compared to medium control. GP + LPS did not change the TNFα production when compared to LPS supplementation or GP/LPS calc. When combined with TSST-1 or GP/TSST-1 calc., GP seemed to exert a synergistic effect on TNFα secretion after 36 h (n = 3; p \u003c 0.05), but overall an enhancement of only 10% was observed (p \u003e 0.05; data not shown in detail).\n\n(vi) IFNγ\nNo IFNγ production was detectable following treatment of PBMC with 100 μg GP. There was a minor increase in IFNγ production following GP + LPS at 36 h, when compared to LPS or GP/LPS calc., and a slight down-regulation following GP + TSST-1 at 24 h and 36 h, when compared to TSST-1 or GP/TSST-1 calc. (data not shown).\n\n(vii) IL-2, IL-12, IL-4, IL-10, TGFβ1\nThe production of IL-2, IL-12, IL-4, IL-10 and TGFβ 1 was not induced by GP. There were also no significant differences between medium control, LPS, TSST-1, GP, GP + LPS, GP/LPS calc. and GP + TSST-1 or GP/TSST-1 calc. over 48 h (data not shown)."}

sentences

simple1

BioNLP16_DUT

BioNLP16_Messiy

DLUT931

bionlp-st-ge-2016-test-ihmc

bionlp-st-ge-2016-spacy-parsed

bionlp-st-ge-2016-test-tees

testone

test3

PMC:1472690 / 10782-16200 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:1472690 / 10782-16200 JSON TXT