PMC:1462997 / 13979-14740 JSONTXT

{"project":"CellFinder","denotations":[{"id":"T770","span":{"begin":352,"end":356},"obj":"GeneProtein"},{"id":"T771","span":{"begin":557,"end":568},"obj":"CellType"},{"id":"T772","span":{"begin":499,"end":506},"obj":"Species"},{"id":"T773","span":{"begin":33,"end":61},"obj":"CellType"},{"id":"T774","span":{"begin":189,"end":192},"obj":"GeneProtein"},{"id":"T775","span":{"begin":478,"end":488},"obj":"CellType"},{"id":"T776","span":{"begin":156,"end":167},"obj":"CellType"},{"id":"T777","span":{"begin":206,"end":210},"obj":"GeneProtein"},{"id":"T778","span":{"begin":316,"end":320},"obj":"GeneProtein"},{"id":"T779","span":{"begin":261,"end":272},"obj":"CellType"},{"id":"T780","span":{"begin":127,"end":134},"obj":"Species"},{"id":"T781","span":{"begin":66,"end":70},"obj":"GeneProtein"},{"id":"T782","span":{"begin":50,"end":61},"obj":"CellType"}],"text":"Figure 3 Functional analysis of hES cell derived macrophages for B7.1 costimulatory molecule upregulation and phagocytosis of E. coli particles: A) Mature macrophages were stimulated with LPS to determine B7.1 upregulation. Twenty-four hours post-stimulation, macrophages were labeled with a PE-CY5 conjugated anti-B7.1 antibody and analyzed by FACS. B7.1 upregulation data are representative of triplicate experiments. Isotype control is shown in the left panel. B) To assess phagocytic function, E. coli Bioparticles® were added directly to the cultured macrophages. Twenty four hours post-addition, cells were analyzed by FACS. Percent positive cells are displayed in the plots for each experiment. These data are representative of triplicate experiments. "}