Id |
Subject |
Object |
Predicate |
Lexical cue |
T22055 |
0-2 |
NN |
denotes |
E2 |
T22056 |
23-33 |
NN |
denotes |
production |
T22057 |
3-7 |
NN |
denotes |
gene |
T22058 |
8-16 |
NN |
denotes |
knockout |
T22059 |
17-22 |
NN |
denotes |
mouse |
T22060 |
33-34 |
. |
denotes |
. |
T22061 |
34-112 |
sentence |
denotes |
A, Gene targeting strategy used for targeting the E2 locus in mouse ES cells. |
T22062 |
35-36 |
LS |
denotes |
A |
T22063 |
53-61 |
NN |
denotes |
strategy |
T22064 |
36-38 |
, |
denotes |
, |
T22065 |
38-42 |
NN |
denotes |
Gene |
T22066 |
43-52 |
NN |
denotes |
targeting |
T22067 |
62-66 |
VBN |
denotes |
used |
T22068 |
67-70 |
IN |
denotes |
for |
T22069 |
71-80 |
VBG |
denotes |
targeting |
T22070 |
81-84 |
DT |
denotes |
the |
T22071 |
88-93 |
NN |
denotes |
locus |
T22072 |
85-87 |
NN |
denotes |
E2 |
T22073 |
94-96 |
IN |
denotes |
in |
T22074 |
97-102 |
NN |
denotes |
mouse |
T22075 |
106-111 |
NNS |
denotes |
cells |
T22076 |
103-105 |
NN |
denotes |
ES |
T22077 |
111-112 |
. |
denotes |
. |
T22078 |
112-240 |
sentence |
denotes |
The targeting construct was designed to delete 1.67 kb of sequence between an EcoRV site in Exon 4 and a Smal site in intron 5. |
T22079 |
113-116 |
DT |
denotes |
The |
T22080 |
127-136 |
NN |
denotes |
construct |
T22081 |
117-126 |
NN |
denotes |
targeting |
T22082 |
141-149 |
VBN |
denotes |
designed |
T22083 |
137-140 |
VBD |
denotes |
was |
T22084 |
150-152 |
TO |
denotes |
to |
T22085 |
153-159 |
VB |
denotes |
delete |
T22086 |
160-164 |
CD |
denotes |
1.67 |
T22087 |
165-167 |
NN |
denotes |
kb |
T22088 |
168-170 |
IN |
denotes |
of |
T22089 |
171-179 |
NN |
denotes |
sequence |
T22090 |
180-187 |
IN |
denotes |
between |
T22091 |
188-190 |
DT |
denotes |
an |
T22092 |
197-201 |
NN |
denotes |
site |
T22093 |
191-196 |
NN |
denotes |
EcoRV |
T22094 |
202-204 |
IN |
denotes |
in |
T22095 |
205-209 |
NN |
denotes |
Exon |
T22096 |
210-211 |
CD |
denotes |
4 |
T22097 |
212-215 |
CC |
denotes |
and |
T22098 |
216-217 |
DT |
denotes |
a |
T22099 |
223-227 |
NN |
denotes |
site |
T22100 |
218-222 |
NN |
denotes |
Smal |
T22101 |
228-230 |
IN |
denotes |
in |
T22102 |
231-237 |
NN |
denotes |
intron |
T22103 |
238-239 |
CD |
denotes |
5 |
T22104 |
239-240 |
. |
denotes |
. |
T22105 |
240-353 |
sentence |
denotes |
The wild type E2 gene contains an ~16 kb BglI restriction fragment that hybridizes to the Exon 6 specific probe. |
T22106 |
241-244 |
DT |
denotes |
The |
T22107 |
258-262 |
NN |
denotes |
gene |
T22108 |
245-249 |
JJ |
denotes |
wild |
T22109 |
250-254 |
NN |
denotes |
type |
T22110 |
255-257 |
NN |
denotes |
E2 |
T22111 |
263-271 |
VBZ |
denotes |
contains |
T22112 |
272-274 |
DT |
denotes |
an |
T22113 |
299-307 |
NN |
denotes |
fragment |
T22114 |
275-276 |
SYM |
denotes |
~ |
T22115 |
276-278 |
CD |
denotes |
16 |
T22116 |
279-281 |
NN |
denotes |
kb |
T22117 |
282-286 |
NN |
denotes |
BglI |
T22118 |
287-298 |
NN |
denotes |
restriction |
T22119 |
308-312 |
WDT |
denotes |
that |
T22120 |
313-323 |
VBZ |
denotes |
hybridizes |
T22121 |
324-326 |
IN |
denotes |
to |
T22122 |
327-330 |
DT |
denotes |
the |
T22123 |
347-352 |
NN |
denotes |
probe |
T22124 |
331-335 |
NN |
denotes |
Exon |
T22125 |
338-346 |
JJ |
denotes |
specific |
T22126 |
336-337 |
CD |
denotes |
6 |
T22127 |
352-353 |
. |
denotes |
. |
T22128 |
353-462 |
sentence |
denotes |
A correctly targeted E2 locus harbors an ~11 kb BglI restriction fragment that hybridizes to the same probe. |
T22129 |
354-355 |
DT |
denotes |
A |
T22130 |
378-383 |
NN |
denotes |
locus |
T22131 |
356-365 |
RB |
denotes |
correctly |
T22132 |
366-374 |
VBN |
denotes |
targeted |
T22133 |
375-377 |
NN |
denotes |
E2 |
T22134 |
384-391 |
VBZ |
denotes |
harbors |
T22135 |
392-394 |
DT |
denotes |
an |
T22136 |
419-427 |
NN |
denotes |
fragment |
T22137 |
395-396 |
SYM |
denotes |
~ |
T22138 |
396-398 |
CD |
denotes |
11 |
T22139 |
399-401 |
NN |
denotes |
kb |
T22140 |
402-406 |
NN |
denotes |
BglI |
T22141 |
407-418 |
NN |
denotes |
restriction |
T22142 |
428-432 |
WDT |
denotes |
that |
T22143 |
433-443 |
VBZ |
denotes |
hybridizes |
T22144 |
444-446 |
IN |
denotes |
to |
T22145 |
447-450 |
DT |
denotes |
the |
T22146 |
456-461 |
NN |
denotes |
probe |
T22147 |
451-455 |
JJ |
denotes |
same |
T22148 |
461-462 |
. |
denotes |
. |
T22149 |
462-589 |
sentence |
denotes |
Note that the probe will not detect random integration of the targeting vector because it is external to the targeting vector. |
T22150 |
463-467 |
VB |
denotes |
Note |
T22151 |
468-472 |
IN |
denotes |
that |
T22152 |
492-498 |
VB |
denotes |
detect |
T22153 |
473-476 |
DT |
denotes |
the |
T22154 |
477-482 |
NN |
denotes |
probe |
T22155 |
483-487 |
MD |
denotes |
will |
T22156 |
488-491 |
RB |
denotes |
not |
T22157 |
499-505 |
JJ |
denotes |
random |
T22158 |
506-517 |
NN |
denotes |
integration |
T22159 |
518-520 |
IN |
denotes |
of |
T22160 |
521-524 |
DT |
denotes |
the |
T22161 |
535-541 |
NN |
denotes |
vector |
T22162 |
525-534 |
NN |
denotes |
targeting |
T22163 |
542-549 |
IN |
denotes |
because |
T22164 |
553-555 |
VBZ |
denotes |
is |
T22165 |
550-552 |
PRP |
denotes |
it |
T22166 |
556-564 |
JJ |
denotes |
external |
T22167 |
565-567 |
IN |
denotes |
to |
T22168 |
568-571 |
DT |
denotes |
the |
T22169 |
582-588 |
NN |
denotes |
vector |
T22170 |
572-581 |
NN |
denotes |
targeting |
T22171 |
588-589 |
. |
denotes |
. |
T22172 |
589-824 |
sentence |
denotes |
B, Southern blot analysis of Bgll digested genomic DNA derived from the parental wild type ES cell line (R1), a heterozygous targeted ES cell line (362), and from wild type (+/+), heterozygous (+/-) and homozygous knockout (-/-) mice. |
T22173 |
590-591 |
LS |
denotes |
B |
T22174 |
607-615 |
NN |
denotes |
analysis |
T22175 |
591-593 |
, |
denotes |
, |
T22176 |
593-601 |
NNP |
denotes |
Southern |
T22177 |
602-606 |
NN |
denotes |
blot |
T22178 |
616-618 |
IN |
denotes |
of |
T22179 |
619-623 |
NN |
denotes |
Bgll |
T22180 |
624-632 |
VBN |
denotes |
digested |
T22181 |
641-644 |
NN |
denotes |
DNA |
T22182 |
633-640 |
JJ |
denotes |
genomic |
T22183 |
645-652 |
VBN |
denotes |
derived |
T22184 |
653-657 |
IN |
denotes |
from |
T22185 |
658-661 |
DT |
denotes |
the |
T22186 |
689-693 |
NN |
denotes |
line |
T22187 |
662-670 |
JJ |
denotes |
parental |
T22188 |
671-675 |
JJ |
denotes |
wild |
T22189 |
676-680 |
NN |
denotes |
type |
T22190 |
681-683 |
NN |
denotes |
ES |
T22191 |
684-688 |
NN |
denotes |
cell |
T22192 |
694-695 |
-LRB- |
denotes |
( |
T22193 |
695-697 |
NN |
denotes |
R1 |
T22194 |
697-698 |
-RRB- |
denotes |
) |
T22195 |
698-700 |
, |
denotes |
, |
T22196 |
700-701 |
DT |
denotes |
a |
T22197 |
732-736 |
NN |
denotes |
line |
T22198 |
702-714 |
JJ |
denotes |
heterozygous |
T22199 |
715-723 |
VBN |
denotes |
targeted |
T22200 |
724-726 |
NN |
denotes |
ES |
T22201 |
727-731 |
NN |
denotes |
cell |
T22202 |
737-738 |
-LRB- |
denotes |
( |
T22203 |
738-741 |
CD |
denotes |
362 |
T22204 |
741-742 |
-RRB- |
denotes |
) |
T22205 |
742-744 |
, |
denotes |
, |
T22206 |
744-747 |
CC |
denotes |
and |
T22207 |
748-752 |
IN |
denotes |
from |
T22208 |
753-757 |
JJ |
denotes |
wild |
T22209 |
758-762 |
NN |
denotes |
type |
T22210 |
804-812 |
NN |
denotes |
knockout |
T22211 |
763-764 |
-LRB- |
denotes |
( |
T22212 |
766-767 |
SYM |
denotes |
+ |
T22213 |
764-765 |
SYM |
denotes |
+ |
T22214 |
765-766 |
HYPH |
denotes |
/ |
T22215 |
767-768 |
-RRB- |
denotes |
) |
T22216 |
768-770 |
, |
denotes |
, |
T22217 |
770-782 |
JJ |
denotes |
heterozygous |
T22218 |
783-784 |
-LRB- |
denotes |
( |
T22219 |
786-787 |
SYM |
denotes |
- |
T22220 |
784-785 |
SYM |
denotes |
+ |
T22221 |
785-786 |
HYPH |
denotes |
/ |
T22222 |
787-788 |
-RRB- |
denotes |
) |
T22223 |
789-792 |
CC |
denotes |
and |
T22224 |
793-803 |
JJ |
denotes |
homozygous |
T22225 |
819-823 |
NNS |
denotes |
mice |
T22226 |
813-814 |
-LRB- |
denotes |
( |
T22227 |
816-817 |
SYM |
denotes |
- |
T22228 |
814-815 |
SYM |
denotes |
- |
T22229 |
815-816 |
HYPH |
denotes |
/ |
T22230 |
817-818 |
-RRB- |
denotes |
) |
T22231 |
823-824 |
. |
denotes |
. |
T22232 |
824-879 |
sentence |
denotes |
The blot was hybridized with an Exon 6 specific probe. |
T22233 |
825-828 |
DT |
denotes |
The |
T22234 |
829-833 |
NN |
denotes |
blot |
T22235 |
838-848 |
VBN |
denotes |
hybridized |
T22236 |
834-837 |
VBD |
denotes |
was |
T22237 |
849-853 |
IN |
denotes |
with |
T22238 |
854-856 |
DT |
denotes |
an |
T22239 |
873-878 |
NN |
denotes |
probe |
T22240 |
857-861 |
NN |
denotes |
Exon |
T22241 |
864-872 |
JJ |
denotes |
specific |
T22242 |
862-863 |
CD |
denotes |
6 |
T22243 |
878-879 |
. |
denotes |
. |
T22244 |
879-1011 |
sentence |
denotes |
C, Immunohistochemical analysis of fresh frozen liver sections from control (+/+) and E2 knockout (-/-) postnatal day 1 mouse pups. |
T22245 |
880-881 |
LS |
denotes |
C |
T22246 |
903-911 |
NN |
denotes |
analysis |
T22247 |
881-883 |
, |
denotes |
, |
T22248 |
883-902 |
JJ |
denotes |
Immunohistochemical |
T22249 |
912-914 |
IN |
denotes |
of |
T22250 |
915-920 |
JJ |
denotes |
fresh |
T22251 |
921-927 |
VBN |
denotes |
frozen |
T22252 |
934-942 |
NNS |
denotes |
sections |
T22253 |
928-933 |
NN |
denotes |
liver |
T22254 |
943-947 |
IN |
denotes |
from |
T22255 |
948-955 |
NN |
denotes |
control |
T22256 |
969-977 |
NN |
denotes |
knockout |
T22257 |
956-957 |
-LRB- |
denotes |
( |
T22258 |
959-960 |
SYM |
denotes |
+ |
T22259 |
957-958 |
SYM |
denotes |
+ |
T22260 |
958-959 |
HYPH |
denotes |
/ |
T22261 |
960-961 |
-RRB- |
denotes |
) |
T22262 |
962-965 |
CC |
denotes |
and |
T22263 |
966-968 |
NN |
denotes |
E2 |
T22264 |
1006-1010 |
NNS |
denotes |
pups |
T22265 |
978-979 |
-LRB- |
denotes |
( |
T22266 |
981-982 |
SYM |
denotes |
- |
T22267 |
979-980 |
SYM |
denotes |
- |
T22268 |
980-981 |
HYPH |
denotes |
/ |
T22269 |
982-983 |
-RRB- |
denotes |
) |
T22270 |
984-993 |
JJ |
denotes |
postnatal |
T22271 |
994-997 |
NN |
denotes |
day |
T22272 |
998-999 |
CD |
denotes |
1 |
T22273 |
1000-1005 |
NN |
denotes |
mouse |
T22274 |
1010-1011 |
. |
denotes |
. |
T22275 |
1011-1106 |
sentence |
denotes |
Sections were stained for E2 using an E2 specific antibody (green) and a nuclear stain (blue). |
T22276 |
1012-1020 |
NNS |
denotes |
Sections |
T22277 |
1026-1033 |
VBN |
denotes |
stained |
T22278 |
1021-1025 |
VBD |
denotes |
were |
T22279 |
1034-1037 |
IN |
denotes |
for |
T22280 |
1038-1040 |
NN |
denotes |
E2 |
T22281 |
1041-1046 |
VBG |
denotes |
using |
T22282 |
1047-1049 |
DT |
denotes |
an |
T22283 |
1062-1070 |
NN |
denotes |
antibody |
T22284 |
1050-1052 |
NN |
denotes |
E2 |
T22285 |
1053-1061 |
JJ |
denotes |
specific |
T22286 |
1071-1072 |
-LRB- |
denotes |
( |
T22287 |
1072-1077 |
JJ |
denotes |
green |
T22288 |
1077-1078 |
-RRB- |
denotes |
) |
T22289 |
1079-1082 |
CC |
denotes |
and |
T22290 |
1083-1084 |
DT |
denotes |
a |
T22291 |
1093-1098 |
NN |
denotes |
stain |
T22292 |
1085-1092 |
JJ |
denotes |
nuclear |
T22293 |
1099-1100 |
-LRB- |
denotes |
( |
T22294 |
1100-1104 |
JJ |
denotes |
blue |
T22295 |
1104-1105 |
-RRB- |
denotes |
) |
T22296 |
1105-1106 |
. |
denotes |
. |
T22297 |
1106-1195 |
sentence |
denotes |
Note the complete absence of E2 immunoreactivity in the section from the knockout mouse. |
T22298 |
1107-1111 |
VB |
denotes |
Note |
T22299 |
1112-1115 |
DT |
denotes |
the |
T22300 |
1125-1132 |
NN |
denotes |
absence |
T22301 |
1116-1124 |
JJ |
denotes |
complete |
T22302 |
1133-1135 |
IN |
denotes |
of |
T22303 |
1136-1138 |
NN |
denotes |
E2 |
T22304 |
1139-1155 |
NN |
denotes |
immunoreactivity |
T22305 |
1156-1158 |
IN |
denotes |
in |
T22306 |
1159-1162 |
DT |
denotes |
the |
T22307 |
1163-1170 |
NN |
denotes |
section |
T22308 |
1171-1175 |
IN |
denotes |
from |
T22309 |
1176-1179 |
DT |
denotes |
the |
T22310 |
1189-1194 |
NN |
denotes |
mouse |
T22311 |
1180-1188 |
NN |
denotes |
knockout |
T22312 |
1194-1195 |
. |
denotes |
. |
T22313 |
1195-1309 |
sentence |
denotes |
D, Similar results were observed upon immunohistochemical analysis of primary mouse embryonic fibroblasts (MEFs). |
T22314 |
1196-1197 |
LS |
denotes |
D |
T22315 |
1220-1228 |
VBN |
denotes |
observed |
T22316 |
1197-1199 |
, |
denotes |
, |
T22317 |
1199-1206 |
JJ |
denotes |
Similar |
T22318 |
1207-1214 |
NNS |
denotes |
results |
T22319 |
1215-1219 |
VBD |
denotes |
were |
T22320 |
1229-1233 |
IN |
denotes |
upon |
T22321 |
1234-1253 |
JJ |
denotes |
immunohistochemical |
T22322 |
1254-1262 |
NN |
denotes |
analysis |
T22323 |
1263-1265 |
IN |
denotes |
of |
T22324 |
1266-1273 |
JJ |
denotes |
primary |
T22325 |
1290-1301 |
NNS |
denotes |
fibroblasts |
T22326 |
1274-1279 |
NN |
denotes |
mouse |
T22327 |
1280-1289 |
JJ |
denotes |
embryonic |
T22328 |
1302-1303 |
-LRB- |
denotes |
( |
T22329 |
1303-1307 |
NNS |
denotes |
MEFs |
T22330 |
1307-1308 |
-RRB- |
denotes |
) |
T22331 |
1308-1309 |
. |
denotes |
. |
T22332 |
1309-1469 |
sentence |
denotes |
Note that the readily detectable signal for E2 in the control cells was present in a pattern characteristic of mitochondria, the subcellular location of BCKDH. |
T22333 |
1310-1314 |
VB |
denotes |
Note |
T22334 |
1315-1319 |
IN |
denotes |
that |
T22335 |
1378-1381 |
VBD |
denotes |
was |
T22336 |
1320-1323 |
DT |
denotes |
the |
T22337 |
1343-1349 |
NN |
denotes |
signal |
T22338 |
1324-1331 |
RB |
denotes |
readily |
T22339 |
1332-1342 |
JJ |
denotes |
detectable |
T22340 |
1350-1353 |
IN |
denotes |
for |
T22341 |
1354-1356 |
NN |
denotes |
E2 |
T22342 |
1357-1359 |
IN |
denotes |
in |
T22343 |
1360-1363 |
DT |
denotes |
the |
T22344 |
1372-1377 |
NNS |
denotes |
cells |
T22345 |
1364-1371 |
NN |
denotes |
control |
T22346 |
1382-1389 |
JJ |
denotes |
present |
T22347 |
1390-1392 |
IN |
denotes |
in |
T22348 |
1393-1394 |
DT |
denotes |
a |
T22349 |
1395-1402 |
NN |
denotes |
pattern |
T22350 |
1403-1417 |
JJ |
denotes |
characteristic |
T22351 |
1418-1420 |
IN |
denotes |
of |
T22352 |
1421-1433 |
NNS |
denotes |
mitochondria |
T22353 |
1433-1435 |
, |
denotes |
, |
T22354 |
1435-1438 |
DT |
denotes |
the |
T22355 |
1451-1459 |
NN |
denotes |
location |
T22356 |
1439-1450 |
JJ |
denotes |
subcellular |
T22357 |
1460-1462 |
IN |
denotes |
of |
T22358 |
1463-1468 |
NN |
denotes |
BCKDH |
T22359 |
1468-1469 |
. |
denotes |
. |
R6272 |
T22055 |
T22056 |
compound |
E2,production |
R6273 |
T22057 |
T22058 |
compound |
gene,knockout |
R6274 |
T22058 |
T22056 |
compound |
knockout,production |
R6275 |
T22059 |
T22056 |
compound |
mouse,production |
R6276 |
T22060 |
T22056 |
punct |
.,production |
R6277 |
T22062 |
T22063 |
meta |
A,strategy |
R6278 |
T22064 |
T22063 |
punct |
", ",strategy |
R6279 |
T22065 |
T22066 |
compound |
Gene,targeting |
R6280 |
T22066 |
T22063 |
compound |
targeting,strategy |
R6281 |
T22067 |
T22063 |
acl |
used,strategy |
R6282 |
T22068 |
T22067 |
prep |
for,used |
R6283 |
T22069 |
T22068 |
pcomp |
targeting,for |
R6284 |
T22070 |
T22071 |
det |
the,locus |
R6285 |
T22071 |
T22069 |
dobj |
locus,targeting |
R6286 |
T22072 |
T22071 |
compound |
E2,locus |
R6287 |
T22073 |
T22067 |
prep |
in,used |
R6288 |
T22074 |
T22075 |
compound |
mouse,cells |
R6289 |
T22075 |
T22073 |
pobj |
cells,in |
R6290 |
T22076 |
T22075 |
compound |
ES,cells |
R6291 |
T22077 |
T22063 |
punct |
.,strategy |
R6292 |
T22079 |
T22080 |
det |
The,construct |
R6293 |
T22080 |
T22082 |
nsubjpass |
construct,designed |
R6294 |
T22081 |
T22080 |
compound |
targeting,construct |
R6295 |
T22083 |
T22082 |
auxpass |
was,designed |
R6296 |
T22084 |
T22085 |
aux |
to,delete |
R6297 |
T22085 |
T22082 |
advcl |
delete,designed |
R6298 |
T22086 |
T22087 |
nummod |
1.67,kb |
R6299 |
T22087 |
T22085 |
dobj |
kb,delete |
R6300 |
T22088 |
T22087 |
prep |
of,kb |
R6301 |
T22089 |
T22088 |
pobj |
sequence,of |
R6302 |
T22090 |
T22085 |
prep |
between,delete |
R6303 |
T22091 |
T22092 |
det |
an,site |
R6304 |
T22092 |
T22090 |
pobj |
site,between |
R6305 |
T22093 |
T22092 |
compound |
EcoRV,site |
R6306 |
T22094 |
T22092 |
prep |
in,site |
R6307 |
T22095 |
T22094 |
pobj |
Exon,in |
R6308 |
T22096 |
T22095 |
nummod |
4,Exon |
R6309 |
T22097 |
T22092 |
cc |
and,site |
R6310 |
T22098 |
T22099 |
det |
a,site |
R6311 |
T22099 |
T22092 |
conj |
site,site |
R6312 |
T22100 |
T22099 |
compound |
Smal,site |
R6313 |
T22101 |
T22099 |
prep |
in,site |
R6314 |
T22102 |
T22101 |
pobj |
intron,in |
R6315 |
T22103 |
T22102 |
nummod |
5,intron |
R6316 |
T22104 |
T22082 |
punct |
.,designed |
R6317 |
T22106 |
T22107 |
det |
The,gene |
R6318 |
T22107 |
T22111 |
nsubj |
gene,contains |
R6319 |
T22108 |
T22109 |
amod |
wild,type |
R6320 |
T22109 |
T22107 |
compound |
type,gene |
R6321 |
T22110 |
T22107 |
compound |
E2,gene |
R6322 |
T22112 |
T22113 |
det |
an,fragment |
R6323 |
T22113 |
T22111 |
dobj |
fragment,contains |
R6324 |
T22114 |
T22115 |
punct |
~,16 |
R6325 |
T22115 |
T22116 |
nummod |
16,kb |
R6326 |
T22116 |
T22113 |
compound |
kb,fragment |
R6327 |
T22117 |
T22113 |
compound |
BglI,fragment |
R6328 |
T22118 |
T22113 |
compound |
restriction,fragment |
R6329 |
T22119 |
T22120 |
dep |
that,hybridizes |
R6330 |
T22120 |
T22113 |
relcl |
hybridizes,fragment |
R6331 |
T22121 |
T22120 |
prep |
to,hybridizes |
R6332 |
T22122 |
T22123 |
det |
the,probe |
R6333 |
T22123 |
T22121 |
pobj |
probe,to |
R6334 |
T22124 |
T22125 |
npadvmod |
Exon,specific |
R6335 |
T22125 |
T22123 |
amod |
specific,probe |
R6336 |
T22126 |
T22124 |
nummod |
6,Exon |
R6337 |
T22127 |
T22111 |
punct |
.,contains |
R6338 |
T22129 |
T22130 |
det |
A,locus |
R6339 |
T22130 |
T22134 |
nsubj |
locus,harbors |
R6340 |
T22131 |
T22132 |
advmod |
correctly,targeted |
R6341 |
T22132 |
T22130 |
amod |
targeted,locus |
R6342 |
T22133 |
T22130 |
compound |
E2,locus |
R6343 |
T22135 |
T22136 |
det |
an,fragment |
R6344 |
T22136 |
T22134 |
dobj |
fragment,harbors |
R6345 |
T22137 |
T22138 |
punct |
~,11 |
R6346 |
T22138 |
T22139 |
nummod |
11,kb |
R6347 |
T22139 |
T22136 |
compound |
kb,fragment |
R6348 |
T22140 |
T22136 |
compound |
BglI,fragment |
R6349 |
T22141 |
T22136 |
compound |
restriction,fragment |
R6350 |
T22142 |
T22143 |
dep |
that,hybridizes |
R6351 |
T22143 |
T22136 |
relcl |
hybridizes,fragment |
R6352 |
T22144 |
T22143 |
prep |
to,hybridizes |
R6353 |
T22145 |
T22146 |
det |
the,probe |
R6354 |
T22146 |
T22144 |
pobj |
probe,to |
R6355 |
T22147 |
T22146 |
amod |
same,probe |
R6356 |
T22148 |
T22134 |
punct |
.,harbors |
R6357 |
T22151 |
T22152 |
mark |
that,detect |
R6358 |
T22152 |
T22150 |
ccomp |
detect,Note |
R6359 |
T22153 |
T22154 |
det |
the,probe |
R6360 |
T22154 |
T22152 |
nsubj |
probe,detect |
R6361 |
T22155 |
T22152 |
aux |
will,detect |
R6362 |
T22156 |
T22152 |
neg |
not,detect |
R6363 |
T22157 |
T22158 |
amod |
random,integration |
R6364 |
T22158 |
T22152 |
dobj |
integration,detect |
R6365 |
T22159 |
T22158 |
prep |
of,integration |
R6366 |
T22160 |
T22161 |
det |
the,vector |
R6367 |
T22161 |
T22159 |
pobj |
vector,of |
R6368 |
T22162 |
T22161 |
compound |
targeting,vector |
R6369 |
T22163 |
T22164 |
mark |
because,is |
R6370 |
T22164 |
T22152 |
advcl |
is,detect |
R6371 |
T22165 |
T22164 |
nsubj |
it,is |
R6372 |
T22166 |
T22164 |
acomp |
external,is |
R6373 |
T22167 |
T22166 |
prep |
to,external |
R6374 |
T22168 |
T22169 |
det |
the,vector |
R6375 |
T22169 |
T22167 |
pobj |
vector,to |
R6376 |
T22170 |
T22169 |
compound |
targeting,vector |
R6377 |
T22171 |
T22150 |
punct |
.,Note |
R6378 |
T22173 |
T22174 |
meta |
B,analysis |
R6379 |
T22175 |
T22174 |
punct |
", ",analysis |
R6380 |
T22176 |
T22177 |
compound |
Southern,blot |
R6381 |
T22177 |
T22174 |
compound |
blot,analysis |
R6382 |
T22178 |
T22174 |
prep |
of,analysis |
R6383 |
T22179 |
T22180 |
npadvmod |
Bgll,digested |
R6384 |
T22180 |
T22181 |
amod |
digested,DNA |
R6385 |
T22181 |
T22178 |
pobj |
DNA,of |
R6386 |
T22182 |
T22181 |
amod |
genomic,DNA |
R6387 |
T22183 |
T22181 |
acl |
derived,DNA |
R6388 |
T22184 |
T22183 |
prep |
from,derived |
R6389 |
T22185 |
T22186 |
det |
the,line |
R6390 |
T22186 |
T22184 |
pobj |
line,from |
R6391 |
T22187 |
T22186 |
amod |
parental,line |
R6392 |
T22188 |
T22189 |
amod |
wild,type |
R6393 |
T22189 |
T22186 |
compound |
type,line |
R6394 |
T22190 |
T22191 |
compound |
ES,cell |
R6395 |
T22191 |
T22186 |
compound |
cell,line |
R6396 |
T22192 |
T22193 |
punct |
(,R1 |
R6397 |
T22193 |
T22186 |
parataxis |
R1,line |
R6398 |
T22194 |
T22193 |
punct |
),R1 |
R6399 |
T22195 |
T22186 |
punct |
", ",line |
R6400 |
T22196 |
T22197 |
det |
a,line |
R6401 |
T22197 |
T22186 |
appos |
line,line |
R6402 |
T22198 |
T22197 |
amod |
heterozygous,line |
R6403 |
T22199 |
T22197 |
amod |
targeted,line |
R6404 |
T22200 |
T22201 |
compound |
ES,cell |
R6405 |
T22201 |
T22197 |
compound |
cell,line |
R6406 |
T22202 |
T22203 |
punct |
(,362 |
R6407 |
T22203 |
T22197 |
parataxis |
362,line |
R6408 |
T22204 |
T22203 |
punct |
),362 |
R6409 |
T22205 |
T22184 |
punct |
", ",from |
R6410 |
T22206 |
T22184 |
cc |
and,from |
R6411 |
T22207 |
T22184 |
conj |
from,from |
R6412 |
T22208 |
T22209 |
amod |
wild,type |
R6413 |
T22209 |
T22210 |
nmod |
type,knockout |
R6414 |
T22210 |
T22225 |
nmod |
knockout,mice |
R6415 |
T22211 |
T22212 |
punct |
(,+ |
R6416 |
T22212 |
T22209 |
punct |
+,type |
R6417 |
T22213 |
T22212 |
punct |
+,+ |
R6418 |
T22214 |
T22212 |
punct |
/,+ |
R6419 |
T22215 |
T22212 |
punct |
),+ |
R6420 |
T22216 |
T22209 |
punct |
", ",type |
R6421 |
T22217 |
T22209 |
conj |
heterozygous,type |
R6422 |
T22218 |
T22219 |
punct |
(,- |
R6423 |
T22219 |
T22217 |
punct |
-,heterozygous |
R6424 |
T22220 |
T22219 |
punct |
+,- |
R6425 |
T22221 |
T22219 |
punct |
/,- |
R6426 |
T22222 |
T22219 |
punct |
),- |
R6427 |
T22223 |
T22217 |
cc |
and,heterozygous |
R6428 |
T22224 |
T22217 |
conj |
homozygous,heterozygous |
R6429 |
T22225 |
T22207 |
pobj |
mice,from |
R6430 |
T22226 |
T22227 |
punct |
(,- |
R6431 |
T22227 |
T22210 |
punct |
-,knockout |
R6432 |
T22228 |
T22227 |
punct |
-,- |
R6433 |
T22229 |
T22227 |
punct |
/,- |
R6434 |
T22230 |
T22227 |
punct |
),- |
R6435 |
T22231 |
T22174 |
punct |
.,analysis |
R6436 |
T22233 |
T22234 |
det |
The,blot |
R6437 |
T22234 |
T22235 |
nsubjpass |
blot,hybridized |
R6438 |
T22236 |
T22235 |
auxpass |
was,hybridized |
R6439 |
T22237 |
T22235 |
prep |
with,hybridized |
R6440 |
T22238 |
T22239 |
det |
an,probe |
R6441 |
T22239 |
T22237 |
pobj |
probe,with |
R6442 |
T22240 |
T22241 |
npadvmod |
Exon,specific |
R6443 |
T22241 |
T22239 |
amod |
specific,probe |
R6444 |
T22242 |
T22240 |
nummod |
6,Exon |
R6445 |
T22243 |
T22235 |
punct |
.,hybridized |
R6446 |
T22245 |
T22246 |
meta |
C,analysis |
R6447 |
T22247 |
T22246 |
punct |
", ",analysis |
R6448 |
T22248 |
T22246 |
amod |
Immunohistochemical,analysis |
R6449 |
T22249 |
T22246 |
prep |
of,analysis |
R6450 |
T22250 |
T22251 |
amod |
fresh,frozen |
R6451 |
T22251 |
T22252 |
amod |
frozen,sections |
R6452 |
T22252 |
T22249 |
pobj |
sections,of |
R6453 |
T22253 |
T22252 |
compound |
liver,sections |
R6454 |
T22254 |
T22252 |
prep |
from,sections |
R6455 |
T22255 |
T22256 |
nmod |
control,knockout |
R6456 |
T22256 |
T22264 |
nmod |
knockout,pups |
R6457 |
T22257 |
T22258 |
punct |
(,+ |
R6458 |
T22258 |
T22255 |
punct |
+,control |
R6459 |
T22259 |
T22258 |
punct |
+,+ |
R6460 |
T22260 |
T22258 |
punct |
/,+ |
R6461 |
T22261 |
T22258 |
punct |
),+ |
R6462 |
T22262 |
T22255 |
cc |
and,control |
R6463 |
T22263 |
T22255 |
conj |
E2,control |
R6464 |
T22264 |
T22254 |
pobj |
pups,from |
R6465 |
T22265 |
T22266 |
punct |
(,- |
R6466 |
T22266 |
T22256 |
punct |
-,knockout |
R6467 |
T22267 |
T22266 |
punct |
-,- |
R6468 |
T22268 |
T22266 |
punct |
/,- |
R6469 |
T22269 |
T22266 |
punct |
),- |
R6470 |
T22270 |
T22271 |
amod |
postnatal,day |
R6471 |
T22271 |
T22264 |
nmod |
day,pups |
R6472 |
T22272 |
T22271 |
nummod |
1,day |
R6473 |
T22273 |
T22264 |
compound |
mouse,pups |
R6474 |
T22274 |
T22246 |
punct |
.,analysis |
R6475 |
T22276 |
T22277 |
nsubjpass |
Sections,stained |
R6476 |
T22278 |
T22277 |
auxpass |
were,stained |
R6477 |
T22279 |
T22277 |
prep |
for,stained |
R6478 |
T22280 |
T22279 |
pobj |
E2,for |
R6479 |
T22281 |
T22277 |
advcl |
using,stained |
R6480 |
T22282 |
T22283 |
det |
an,antibody |
R6481 |
T22283 |
T22281 |
dobj |
antibody,using |
R6482 |
T22284 |
T22285 |
npadvmod |
E2,specific |
R6483 |
T22285 |
T22283 |
amod |
specific,antibody |
R6484 |
T22286 |
T22287 |
punct |
(,green |
R6485 |
T22287 |
T22283 |
parataxis |
green,antibody |
R6486 |
T22288 |
T22287 |
punct |
),green |
R6487 |
T22289 |
T22283 |
cc |
and,antibody |
R6488 |
T22290 |
T22291 |
det |
a,stain |
R6489 |
T22291 |
T22283 |
conj |
stain,antibody |
R6490 |
T22292 |
T22291 |
amod |
nuclear,stain |
R6491 |
T22293 |
T22294 |
punct |
(,blue |
R6492 |
T22294 |
T22291 |
parataxis |
blue,stain |
R6493 |
T22295 |
T22294 |
punct |
),blue |
R6494 |
T22296 |
T22277 |
punct |
.,stained |
R6495 |
T22299 |
T22300 |
det |
the,absence |
R6496 |
T22300 |
T22298 |
dobj |
absence,Note |
R6497 |
T22301 |
T22300 |
amod |
complete,absence |
R6498 |
T22302 |
T22300 |
prep |
of,absence |
R6499 |
T22303 |
T22304 |
compound |
E2,immunoreactivity |
R6500 |
T22304 |
T22302 |
pobj |
immunoreactivity,of |
R6501 |
T22305 |
T22298 |
prep |
in,Note |
R6502 |
T22306 |
T22307 |
det |
the,section |
R6503 |
T22307 |
T22305 |
pobj |
section,in |
R6504 |
T22308 |
T22307 |
prep |
from,section |
R6505 |
T22309 |
T22310 |
det |
the,mouse |
R6506 |
T22310 |
T22308 |
pobj |
mouse,from |
R6507 |
T22311 |
T22310 |
compound |
knockout,mouse |
R6508 |
T22312 |
T22298 |
punct |
.,Note |
R6509 |
T22314 |
T22315 |
meta |
D,observed |
R6510 |
T22316 |
T22315 |
punct |
", ",observed |
R6511 |
T22317 |
T22318 |
amod |
Similar,results |
R6512 |
T22318 |
T22315 |
nsubjpass |
results,observed |
R6513 |
T22319 |
T22315 |
auxpass |
were,observed |
R6514 |
T22320 |
T22315 |
prep |
upon,observed |
R6515 |
T22321 |
T22322 |
amod |
immunohistochemical,analysis |
R6516 |
T22322 |
T22320 |
pobj |
analysis,upon |
R6517 |
T22323 |
T22322 |
prep |
of,analysis |
R6518 |
T22324 |
T22325 |
amod |
primary,fibroblasts |
R6519 |
T22325 |
T22323 |
pobj |
fibroblasts,of |
R6520 |
T22326 |
T22325 |
nmod |
mouse,fibroblasts |
R6521 |
T22327 |
T22325 |
amod |
embryonic,fibroblasts |
R6522 |
T22328 |
T22325 |
punct |
(,fibroblasts |
R6523 |
T22329 |
T22325 |
appos |
MEFs,fibroblasts |
R6524 |
T22330 |
T22315 |
punct |
),observed |
R6525 |
T22331 |
T22315 |
punct |
.,observed |
R6526 |
T22334 |
T22335 |
mark |
that,was |
R6527 |
T22335 |
T22333 |
ccomp |
was,Note |
R6528 |
T22336 |
T22337 |
det |
the,signal |
R6529 |
T22337 |
T22335 |
nsubj |
signal,was |
R6530 |
T22338 |
T22339 |
advmod |
readily,detectable |
R6531 |
T22339 |
T22337 |
amod |
detectable,signal |
R6532 |
T22340 |
T22337 |
prep |
for,signal |
R6533 |
T22341 |
T22340 |
pobj |
E2,for |
R6534 |
T22342 |
T22337 |
prep |
in,signal |
R6535 |
T22343 |
T22344 |
det |
the,cells |
R6536 |
T22344 |
T22342 |
pobj |
cells,in |
R6537 |
T22345 |
T22344 |
compound |
control,cells |
R6538 |
T22346 |
T22335 |
acomp |
present,was |
R6539 |
T22347 |
T22346 |
prep |
in,present |
R6540 |
T22348 |
T22349 |
det |
a,pattern |
R6541 |
T22349 |
T22347 |
pobj |
pattern,in |
R6542 |
T22350 |
T22349 |
amod |
characteristic,pattern |
R6543 |
T22351 |
T22350 |
prep |
of,characteristic |
R6544 |
T22352 |
T22351 |
pobj |
mitochondria,of |
R6545 |
T22353 |
T22352 |
punct |
", ",mitochondria |
R6546 |
T22354 |
T22355 |
det |
the,location |
R6547 |
T22355 |
T22352 |
appos |
location,mitochondria |
R6548 |
T22356 |
T22355 |
amod |
subcellular,location |
R6549 |
T22357 |
T22355 |
prep |
of,location |
R6550 |
T22358 |
T22357 |
pobj |
BCKDH,of |
R6551 |
T22359 |
T22333 |
punct |
.,Note |