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Id Subject Object Predicate Lexical cue
T5027 0-4 NN denotes Gene
T5028 5-13 NN denotes knockout
T5029 14-24 NN denotes production
T5030 24-250 sentence denotes A genomic DNA subclone of Strain 129/SvJ DNA that contained a portion of Exons 4 and 5 of the E2 gene and flanking DNA was obtained from a P1 phage library from Genome Systems, Inc., (St. Louis, MO; '3-Hit Mouse ES Library').
T5031 25-26 DT denotes A
T5032 39-47 NN denotes subclone
T5033 27-34 JJ denotes genomic
T5034 35-38 NN denotes DNA
T5035 148-156 VBN denotes obtained
T5036 48-50 IN denotes of
T5037 51-57 NN denotes Strain
T5038 66-69 NN denotes DNA
T5039 58-61 CD denotes 129
T5040 62-65 NN denotes SvJ
T5041 61-62 HYPH denotes /
T5042 70-74 WDT denotes that
T5043 75-84 VBD denotes contained
T5044 85-86 DT denotes a
T5045 87-94 NN denotes portion
T5046 95-97 IN denotes of
T5047 98-103 NNS denotes Exons
T5048 104-105 CD denotes 4
T5049 106-109 CC denotes and
T5050 110-111 CD denotes 5
T5051 112-114 IN denotes of
T5052 115-118 DT denotes the
T5053 122-126 NN denotes gene
T5054 119-121 NN denotes E2
T5055 127-130 CC denotes and
T5056 131-139 NN denotes flanking
T5057 140-143 NN denotes DNA
T5058 144-147 VBD denotes was
T5059 157-161 IN denotes from
T5060 162-163 DT denotes a
T5061 173-180 NN denotes library
T5062 164-166 NN denotes P1
T5063 167-172 NN denotes phage
T5064 181-185 IN denotes from
T5065 186-192 NNP denotes Genome
T5066 202-206 NNP denotes Inc.
T5067 193-200 NNP denotes Systems
T5068 200-202 , denotes ,
T5069 206-208 , denotes ,
T5070 208-209 -LRB- denotes (
T5071 240-247 NNP denotes Library
T5072 209-212 NNP denotes St.
T5073 213-218 NNP denotes Louis
T5074 218-220 , denotes ,
T5075 220-222 NNP denotes MO
T5076 222-223 : denotes ;
T5077 224-225 SYM denotes '
T5078 225-226 CD denotes 3
T5079 227-230 NN denotes Hit
T5080 226-227 HYPH denotes -
T5081 231-236 NNP denotes Mouse
T5082 237-239 NNP denotes ES
T5083 247-248 POS denotes '
T5084 248-249 -RRB- denotes )
T5085 249-250 . denotes .
T5086 250-490 sentence denotes A positive/negative replacement type gene targeting vector [21] was created by replacing a 1.67 kb EcoRV-Smal fragment that included a portion of Exon 4 and all of Exon 5 with the PGKneo marker gene (See Fig. 1A) from the pPNT vector [22].
T5087 251-252 DT denotes A
T5088 303-309 NN denotes vector
T5089 253-261 JJ denotes positive
T5090 262-270 JJ denotes negative
T5091 261-262 HYPH denotes /
T5092 271-282 NN denotes replacement
T5093 283-287 NN denotes type
T5094 288-292 NN denotes gene
T5095 293-302 NN denotes targeting
T5096 319-326 VBN denotes created
T5097 310-311 -LRB- denotes [
T5098 311-313 CD denotes 21
T5099 313-314 -RRB- denotes ]
T5100 315-318 VBD denotes was
T5101 327-329 IN denotes by
T5102 330-339 VBG denotes replacing
T5103 340-341 DT denotes a
T5104 361-369 NN denotes fragment
T5105 342-346 CD denotes 1.67
T5106 347-349 NN denotes kb
T5107 350-355 NN denotes EcoRV
T5108 356-360 NN denotes Smal
T5109 355-356 HYPH denotes -
T5110 370-374 WDT denotes that
T5111 375-383 VBD denotes included
T5112 384-385 DT denotes a
T5113 386-393 NN denotes portion
T5114 394-396 IN denotes of
T5115 397-401 NN denotes Exon
T5116 402-403 CD denotes 4
T5117 404-407 CC denotes and
T5118 408-411 DT denotes all
T5119 412-414 IN denotes of
T5120 415-419 NN denotes Exon
T5121 420-421 CD denotes 5
T5122 422-426 IN denotes with
T5123 427-430 DT denotes the
T5124 445-449 NN denotes gene
T5125 431-437 NN denotes PGKneo
T5126 438-444 NN denotes marker
T5127 450-451 -LRB- denotes (
T5128 451-454 VB denotes See
T5129 455-459 NN denotes Fig.
T5130 460-462 NN denotes 1A
T5131 462-463 -RRB- denotes )
T5132 464-468 IN denotes from
T5133 469-472 DT denotes the
T5134 478-484 NN denotes vector
T5135 473-477 NN denotes pPNT
T5136 485-486 -LRB- denotes [
T5137 486-488 CD denotes 22
T5138 488-489 -RRB- denotes ]
T5139 489-490 . denotes .
T5140 490-624 sentence denotes The targeting construct was linearized with NotI and electroporated into R1 ES cells [23] under conditions described previously [24].
T5141 491-494 DT denotes The
T5142 505-514 NN denotes construct
T5143 495-504 NN denotes targeting
T5144 519-529 VBN denotes linearized
T5145 515-518 VBD denotes was
T5146 530-534 IN denotes with
T5147 535-539 NN denotes NotI
T5148 540-543 CC denotes and
T5149 544-558 VBN denotes electroporated
T5150 559-563 IN denotes into
T5151 564-566 NN denotes R1
T5152 570-575 NNS denotes cells
T5153 567-569 NN denotes ES
T5154 576-577 -LRB- denotes [
T5155 577-579 CD denotes 23
T5156 579-580 -RRB- denotes ]
T5157 581-586 IN denotes under
T5158 587-597 NNS denotes conditions
T5159 598-607 VBN denotes described
T5160 608-618 RB denotes previously
T5161 619-620 -LRB- denotes [
T5162 620-622 CD denotes 24
T5163 622-623 -RRB- denotes ]
T5164 623-624 . denotes .
T5165 624-766 sentence denotes ES cells were selected with G418 (300 μg/ml; Life Technologies Inc., Gaithersburg, MD) and gancyclovir (2 μM; gift of Syntex, Palo Alto, CA).
T5166 625-627 NN denotes ES
T5167 628-633 NNS denotes cells
T5168 639-647 VBN denotes selected
T5169 634-638 VBD denotes were
T5170 648-652 IN denotes with
T5171 653-657 NN denotes G418
T5172 658-659 -LRB- denotes (
T5173 688-692 NNP denotes Inc.
T5174 659-662 CD denotes 300
T5175 663-665 NN denotes μg
T5176 665-666 SYM denotes /
T5177 666-668 NN denotes ml
T5178 668-669 : denotes ;
T5179 670-674 NNP denotes Life
T5180 675-687 NNP denotes Technologies
T5181 692-694 , denotes ,
T5182 694-706 NNP denotes Gaithersburg
T5183 706-708 , denotes ,
T5184 708-710 NNP denotes MD
T5185 710-711 -RRB- denotes )
T5186 712-715 CC denotes and
T5187 716-727 NN denotes gancyclovir
T5188 728-729 -LRB- denotes (
T5189 729-730 CD denotes 2
T5190 731-733 NN denotes μM
T5191 733-734 : denotes ;
T5192 735-739 NN denotes gift
T5193 740-742 IN denotes of
T5194 743-749 NNP denotes Syntex
T5195 749-751 , denotes ,
T5196 751-755 NNP denotes Palo
T5197 756-760 NNP denotes Alto
T5198 760-762 , denotes ,
T5199 762-764 NNP denotes CA
T5200 764-765 -RRB- denotes )
T5201 765-766 . denotes .
T5202 766-879 sentence denotes Doubly resistant clones were screened for gene targeting by Southern blot analysis of BglI digested genomic DNA.
T5203 767-773 RB denotes Doubly
T5204 774-783 JJ denotes resistant
T5205 784-790 NNS denotes clones
T5206 796-804 VBN denotes screened
T5207 791-795 VBD denotes were
T5208 805-808 IN denotes for
T5209 809-813 NN denotes gene
T5210 814-823 NN denotes targeting
T5211 824-826 IN denotes by
T5212 827-835 NNP denotes Southern
T5213 836-840 NN denotes blot
T5214 841-849 NN denotes analysis
T5215 850-852 IN denotes of
T5216 853-857 NN denotes BglI
T5217 875-878 NN denotes DNA
T5218 858-866 VBN denotes digested
T5219 867-874 JJ denotes genomic
T5220 878-879 . denotes .
T5221 879-982 sentence denotes Blots were hybridized with an Exon 6 specific probe that was external to the gene targeting construct.
T5222 880-885 NNS denotes Blots
T5223 891-901 VBN denotes hybridized
T5224 886-890 VBD denotes were
T5225 902-906 IN denotes with
T5226 907-909 DT denotes an
T5227 926-931 NN denotes probe
T5228 910-914 NN denotes Exon
T5229 917-925 JJ denotes specific
T5230 915-916 CD denotes 6
T5231 932-936 WDT denotes that
T5232 937-940 VBD denotes was
T5233 941-949 JJ denotes external
T5234 950-952 IN denotes to
T5235 953-956 DT denotes the
T5236 972-981 NN denotes construct
T5237 957-961 NN denotes gene
T5238 962-971 NN denotes targeting
T5239 981-982 . denotes .
T5240 982-1102 sentence denotes Correctly targeted ES cells were injected into C57BL/6J blastocysts to produce chimeric mice using standard procedures.
T5241 983-992 RB denotes Correctly
T5242 993-1001 VBN denotes targeted
T5243 1005-1010 NNS denotes cells
T5244 1002-1004 NN denotes ES
T5245 1016-1024 VBN denotes injected
T5246 1011-1015 VBD denotes were
T5247 1025-1029 IN denotes into
T5248 1030-1035 NN denotes C57BL
T5249 1036-1038 NN denotes 6J
T5250 1035-1036 HYPH denotes /
T5251 1039-1050 NNS denotes blastocysts
T5252 1051-1053 TO denotes to
T5253 1054-1061 VB denotes produce
T5254 1062-1070 JJ denotes chimeric
T5255 1071-1075 NNS denotes mice
T5256 1076-1081 VBG denotes using
T5257 1082-1090 JJ denotes standard
T5258 1091-1101 NNS denotes procedures
T5259 1101-1102 . denotes .
T5260 1102-1259 sentence denotes Heterozygous offspring from germline competent chimeras were intercrossed to produce wild type (+/+), heterozygous (+/-) and homozygous knockout (-/-) mice.
T5261 1103-1115 JJ denotes Heterozygous
T5262 1116-1125 NN denotes offspring
T5263 1164-1176 VBN denotes intercrossed
T5264 1126-1130 IN denotes from
T5265 1131-1139 NN denotes germline
T5266 1140-1149 JJ denotes competent
T5267 1150-1158 NNS denotes chimeras
T5268 1159-1163 VBD denotes were
T5269 1177-1179 TO denotes to
T5270 1180-1187 VB denotes produce
T5271 1188-1192 JJ denotes wild
T5272 1193-1197 NN denotes type
T5273 1239-1247 NN denotes knockout
T5274 1198-1199 -LRB- denotes (
T5275 1201-1202 SYM denotes +
T5276 1199-1200 SYM denotes +
T5277 1200-1201 HYPH denotes /
T5278 1202-1203 -RRB- denotes )
T5279 1203-1205 , denotes ,
T5280 1205-1217 JJ denotes heterozygous
T5281 1218-1219 -LRB- denotes (
T5282 1221-1222 SYM denotes -
T5283 1219-1220 SYM denotes +
T5284 1220-1221 HYPH denotes /
T5285 1222-1223 -RRB- denotes )
T5286 1224-1227 CC denotes and
T5287 1228-1238 JJ denotes homozygous
T5288 1254-1258 NNS denotes mice
T5289 1248-1249 -LRB- denotes (
T5290 1251-1252 SYM denotes -
T5291 1249-1250 SYM denotes -
T5292 1250-1251 HYPH denotes /
T5293 1252-1253 -RRB- denotes )
T5294 1258-1259 . denotes .
T5295 1259-1309 sentence denotes At all generations, +/- breeding pairs were used.
T5296 1260-1262 IN denotes At
T5297 1304-1308 VBN denotes used
T5298 1263-1266 DT denotes all
T5299 1267-1278 NNS denotes generations
T5300 1278-1280 , denotes ,
T5301 1280-1281 SYM denotes +
T5302 1282-1283 SYM denotes -
T5303 1281-1282 HYPH denotes /
T5304 1293-1298 NNS denotes pairs
T5305 1284-1292 VBG denotes breeding
T5306 1299-1303 VBD denotes were
T5307 1308-1309 . denotes .
T5308 1309-1380 sentence denotes Results presented here are from mice derived from the F2+ generations.
T5309 1310-1317 NNS denotes Results
T5310 1333-1336 VBP denotes are
T5311 1318-1327 VBN denotes presented
T5312 1328-1332 RB denotes here
T5313 1337-1341 IN denotes from
T5314 1342-1346 NNS denotes mice
T5315 1347-1354 VBN denotes derived
T5316 1355-1359 IN denotes from
T5317 1360-1363 DT denotes the
T5318 1368-1379 NNS denotes generations
T5319 1364-1366 NN denotes F2
T5320 1366-1367 SYM denotes +
T5321 1379-1380 . denotes .
T5322 1380-1456 sentence denotes All animals were of a mixed C57BL/6J × Strain 129Sv/SvJ genetic background.
T5323 1381-1384 DT denotes All
T5324 1385-1392 NNS denotes animals
T5325 1393-1397 VBD denotes were
T5326 1398-1400 IN denotes of
T5327 1401-1402 DT denotes a
T5328 1445-1455 NN denotes background
T5329 1403-1408 VBN denotes mixed
T5330 1409-1414 NN denotes C57BL
T5331 1415-1417 NN denotes 6J
T5332 1414-1415 HYPH denotes /
T5333 1418-1419 SYM denotes ×
T5334 1420-1426 NN denotes Strain
T5335 1433-1436 NN denotes SvJ
T5336 1427-1432 NN denotes 129Sv
T5337 1432-1433 HYPH denotes /
T5338 1437-1444 JJ denotes genetic
T5339 1455-1456 . denotes .
T22055 1467-1469 NN denotes E2
T22056 1490-1500 NN denotes production
T22057 1470-1474 NN denotes gene
T22058 1475-1483 NN denotes knockout
T22059 1484-1489 NN denotes mouse
T22060 1500-1501 . denotes .
T22061 1501-1579 sentence denotes A, Gene targeting strategy used for targeting the E2 locus in mouse ES cells.
T22062 1502-1503 LS denotes A
T22063 1520-1528 NN denotes strategy
T22064 1503-1505 , denotes ,
T22065 1505-1509 NN denotes Gene
T22066 1510-1519 NN denotes targeting
T22067 1529-1533 VBN denotes used
T22068 1534-1537 IN denotes for
T22069 1538-1547 VBG denotes targeting
T22070 1548-1551 DT denotes the
T22071 1555-1560 NN denotes locus
T22072 1552-1554 NN denotes E2
T22073 1561-1563 IN denotes in
T22074 1564-1569 NN denotes mouse
T22075 1573-1578 NNS denotes cells
T22076 1570-1572 NN denotes ES
T22077 1578-1579 . denotes .
T22078 1579-1707 sentence denotes The targeting construct was designed to delete 1.67 kb of sequence between an EcoRV site in Exon 4 and a Smal site in intron 5.
T22079 1580-1583 DT denotes The
T22080 1594-1603 NN denotes construct
T22081 1584-1593 NN denotes targeting
T22082 1608-1616 VBN denotes designed
T22083 1604-1607 VBD denotes was
T22084 1617-1619 TO denotes to
T22085 1620-1626 VB denotes delete
T22086 1627-1631 CD denotes 1.67
T22087 1632-1634 NN denotes kb
T22088 1635-1637 IN denotes of
T22089 1638-1646 NN denotes sequence
T22090 1647-1654 IN denotes between
T22091 1655-1657 DT denotes an
T22092 1664-1668 NN denotes site
T22093 1658-1663 NN denotes EcoRV
T22094 1669-1671 IN denotes in
T22095 1672-1676 NN denotes Exon
T22096 1677-1678 CD denotes 4
T22097 1679-1682 CC denotes and
T22098 1683-1684 DT denotes a
T22099 1690-1694 NN denotes site
T22100 1685-1689 NN denotes Smal
T22101 1695-1697 IN denotes in
T22102 1698-1704 NN denotes intron
T22103 1705-1706 CD denotes 5
T22104 1706-1707 . denotes .
T22105 1707-1820 sentence denotes The wild type E2 gene contains an ~16 kb BglI restriction fragment that hybridizes to the Exon 6 specific probe.
T22106 1708-1711 DT denotes The
T22107 1725-1729 NN denotes gene
T22108 1712-1716 JJ denotes wild
T22109 1717-1721 NN denotes type
T22110 1722-1724 NN denotes E2
T22111 1730-1738 VBZ denotes contains
T22112 1739-1741 DT denotes an
T22113 1766-1774 NN denotes fragment
T22114 1742-1743 SYM denotes ~
T22115 1743-1745 CD denotes 16
T22116 1746-1748 NN denotes kb
T22117 1749-1753 NN denotes BglI
T22118 1754-1765 NN denotes restriction
T22119 1775-1779 WDT denotes that
T22120 1780-1790 VBZ denotes hybridizes
T22121 1791-1793 IN denotes to
T22122 1794-1797 DT denotes the
T22123 1814-1819 NN denotes probe
T22124 1798-1802 NN denotes Exon
T22125 1805-1813 JJ denotes specific
T22126 1803-1804 CD denotes 6
T22127 1819-1820 . denotes .
T22128 1820-1929 sentence denotes A correctly targeted E2 locus harbors an ~11 kb BglI restriction fragment that hybridizes to the same probe.
T22129 1821-1822 DT denotes A
T22130 1845-1850 NN denotes locus
T22131 1823-1832 RB denotes correctly
T22132 1833-1841 VBN denotes targeted
T22133 1842-1844 NN denotes E2
T22134 1851-1858 VBZ denotes harbors
T22135 1859-1861 DT denotes an
T22136 1886-1894 NN denotes fragment
T22137 1862-1863 SYM denotes ~
T22138 1863-1865 CD denotes 11
T22139 1866-1868 NN denotes kb
T22140 1869-1873 NN denotes BglI
T22141 1874-1885 NN denotes restriction
T22142 1895-1899 WDT denotes that
T22143 1900-1910 VBZ denotes hybridizes
T22144 1911-1913 IN denotes to
T22145 1914-1917 DT denotes the
T22146 1923-1928 NN denotes probe
T22147 1918-1922 JJ denotes same
T22148 1928-1929 . denotes .
T22149 1929-2056 sentence denotes Note that the probe will not detect random integration of the targeting vector because it is external to the targeting vector.
T22150 1930-1934 VB denotes Note
T22151 1935-1939 IN denotes that
T22152 1959-1965 VB denotes detect
T22153 1940-1943 DT denotes the
T22154 1944-1949 NN denotes probe
T22155 1950-1954 MD denotes will
T22156 1955-1958 RB denotes not
T22157 1966-1972 JJ denotes random
T22158 1973-1984 NN denotes integration
T22159 1985-1987 IN denotes of
T22160 1988-1991 DT denotes the
T22161 2002-2008 NN denotes vector
T22162 1992-2001 NN denotes targeting
T22163 2009-2016 IN denotes because
T22164 2020-2022 VBZ denotes is
T22165 2017-2019 PRP denotes it
T22166 2023-2031 JJ denotes external
T22167 2032-2034 IN denotes to
T22168 2035-2038 DT denotes the
T22169 2049-2055 NN denotes vector
T22170 2039-2048 NN denotes targeting
T22171 2055-2056 . denotes .
T22172 2056-2291 sentence denotes B, Southern blot analysis of Bgll digested genomic DNA derived from the parental wild type ES cell line (R1), a heterozygous targeted ES cell line (362), and from wild type (+/+), heterozygous (+/-) and homozygous knockout (-/-) mice.
T22173 2057-2058 LS denotes B
T22174 2074-2082 NN denotes analysis
T22175 2058-2060 , denotes ,
T22176 2060-2068 NNP denotes Southern
T22177 2069-2073 NN denotes blot
T22178 2083-2085 IN denotes of
T22179 2086-2090 NN denotes Bgll
T22180 2091-2099 VBN denotes digested
T22181 2108-2111 NN denotes DNA
T22182 2100-2107 JJ denotes genomic
T22183 2112-2119 VBN denotes derived
T22184 2120-2124 IN denotes from
T22185 2125-2128 DT denotes the
T22186 2156-2160 NN denotes line
T22187 2129-2137 JJ denotes parental
T22188 2138-2142 JJ denotes wild
T22189 2143-2147 NN denotes type
T22190 2148-2150 NN denotes ES
T22191 2151-2155 NN denotes cell
T22192 2161-2162 -LRB- denotes (
T22193 2162-2164 NN denotes R1
T22194 2164-2165 -RRB- denotes )
T22195 2165-2167 , denotes ,
T22196 2167-2168 DT denotes a
T22197 2199-2203 NN denotes line
T22198 2169-2181 JJ denotes heterozygous
T22199 2182-2190 VBN denotes targeted
T22200 2191-2193 NN denotes ES
T22201 2194-2198 NN denotes cell
T22202 2204-2205 -LRB- denotes (
T22203 2205-2208 CD denotes 362
T22204 2208-2209 -RRB- denotes )
T22205 2209-2211 , denotes ,
T22206 2211-2214 CC denotes and
T22207 2215-2219 IN denotes from
T22208 2220-2224 JJ denotes wild
T22209 2225-2229 NN denotes type
T22210 2271-2279 NN denotes knockout
T22211 2230-2231 -LRB- denotes (
T22212 2233-2234 SYM denotes +
T22213 2231-2232 SYM denotes +
T22214 2232-2233 HYPH denotes /
T22215 2234-2235 -RRB- denotes )
T22216 2235-2237 , denotes ,
T22217 2237-2249 JJ denotes heterozygous
T22218 2250-2251 -LRB- denotes (
T22219 2253-2254 SYM denotes -
T22220 2251-2252 SYM denotes +
T22221 2252-2253 HYPH denotes /
T22222 2254-2255 -RRB- denotes )
T22223 2256-2259 CC denotes and
T22224 2260-2270 JJ denotes homozygous
T22225 2286-2290 NNS denotes mice
T22226 2280-2281 -LRB- denotes (
T22227 2283-2284 SYM denotes -
T22228 2281-2282 SYM denotes -
T22229 2282-2283 HYPH denotes /
T22230 2284-2285 -RRB- denotes )
T22231 2290-2291 . denotes .
T22232 2291-2346 sentence denotes The blot was hybridized with an Exon 6 specific probe.
T22233 2292-2295 DT denotes The
T22234 2296-2300 NN denotes blot
T22235 2305-2315 VBN denotes hybridized
T22236 2301-2304 VBD denotes was
T22237 2316-2320 IN denotes with
T22238 2321-2323 DT denotes an
T22239 2340-2345 NN denotes probe
T22240 2324-2328 NN denotes Exon
T22241 2331-2339 JJ denotes specific
T22242 2329-2330 CD denotes 6
T22243 2345-2346 . denotes .
T22244 2346-2478 sentence denotes C, Immunohistochemical analysis of fresh frozen liver sections from control (+/+) and E2 knockout (-/-) postnatal day 1 mouse pups.
T22245 2347-2348 LS denotes C
T22246 2370-2378 NN denotes analysis
T22247 2348-2350 , denotes ,
T22248 2350-2369 JJ denotes Immunohistochemical
T22249 2379-2381 IN denotes of
T22250 2382-2387 JJ denotes fresh
T22251 2388-2394 VBN denotes frozen
T22252 2401-2409 NNS denotes sections
T22253 2395-2400 NN denotes liver
T22254 2410-2414 IN denotes from
T22255 2415-2422 NN denotes control
T22256 2436-2444 NN denotes knockout
T22257 2423-2424 -LRB- denotes (
T22258 2426-2427 SYM denotes +
T22259 2424-2425 SYM denotes +
T22260 2425-2426 HYPH denotes /
T22261 2427-2428 -RRB- denotes )
T22262 2429-2432 CC denotes and
T22263 2433-2435 NN denotes E2
T22264 2473-2477 NNS denotes pups
T22265 2445-2446 -LRB- denotes (
T22266 2448-2449 SYM denotes -
T22267 2446-2447 SYM denotes -
T22268 2447-2448 HYPH denotes /
T22269 2449-2450 -RRB- denotes )
T22270 2451-2460 JJ denotes postnatal
T22271 2461-2464 NN denotes day
T22272 2465-2466 CD denotes 1
T22273 2467-2472 NN denotes mouse
T22274 2477-2478 . denotes .
T22275 2478-2573 sentence denotes Sections were stained for E2 using an E2 specific antibody (green) and a nuclear stain (blue).
T22276 2479-2487 NNS denotes Sections
T22277 2493-2500 VBN denotes stained
T22278 2488-2492 VBD denotes were
T22279 2501-2504 IN denotes for
T22280 2505-2507 NN denotes E2
T22281 2508-2513 VBG denotes using
T22282 2514-2516 DT denotes an
T22283 2529-2537 NN denotes antibody
T22284 2517-2519 NN denotes E2
T22285 2520-2528 JJ denotes specific
T22286 2538-2539 -LRB- denotes (
T22287 2539-2544 JJ denotes green
T22288 2544-2545 -RRB- denotes )
T22289 2546-2549 CC denotes and
T22290 2550-2551 DT denotes a
T22291 2560-2565 NN denotes stain
T22292 2552-2559 JJ denotes nuclear
T22293 2566-2567 -LRB- denotes (
T22294 2567-2571 JJ denotes blue
T22295 2571-2572 -RRB- denotes )
T22296 2572-2573 . denotes .
T22297 2573-2662 sentence denotes Note the complete absence of E2 immunoreactivity in the section from the knockout mouse.
T22298 2574-2578 VB denotes Note
T22299 2579-2582 DT denotes the
T22300 2592-2599 NN denotes absence
T22301 2583-2591 JJ denotes complete
T22302 2600-2602 IN denotes of
T22303 2603-2605 NN denotes E2
T22304 2606-2622 NN denotes immunoreactivity
T22305 2623-2625 IN denotes in
T22306 2626-2629 DT denotes the
T22307 2630-2637 NN denotes section
T22308 2638-2642 IN denotes from
T22309 2643-2646 DT denotes the
T22310 2656-2661 NN denotes mouse
T22311 2647-2655 NN denotes knockout
T22312 2661-2662 . denotes .
T22313 2662-2776 sentence denotes D, Similar results were observed upon immunohistochemical analysis of primary mouse embryonic fibroblasts (MEFs).
T22314 2663-2664 LS denotes D
T22315 2687-2695 VBN denotes observed
T22316 2664-2666 , denotes ,
T22317 2666-2673 JJ denotes Similar
T22318 2674-2681 NNS denotes results
T22319 2682-2686 VBD denotes were
T22320 2696-2700 IN denotes upon
T22321 2701-2720 JJ denotes immunohistochemical
T22322 2721-2729 NN denotes analysis
T22323 2730-2732 IN denotes of
T22324 2733-2740 JJ denotes primary
T22325 2757-2768 NNS denotes fibroblasts
T22326 2741-2746 NN denotes mouse
T22327 2747-2756 JJ denotes embryonic
T22328 2769-2770 -LRB- denotes (
T22329 2770-2774 NNS denotes MEFs
T22330 2774-2775 -RRB- denotes )
T22331 2775-2776 . denotes .
T22332 2776-2936 sentence denotes Note that the readily detectable signal for E2 in the control cells was present in a pattern characteristic of mitochondria, the subcellular location of BCKDH.
T22333 2777-2781 VB denotes Note
T22334 2782-2786 IN denotes that
T22335 2845-2848 VBD denotes was
T22336 2787-2790 DT denotes the
T22337 2810-2816 NN denotes signal
T22338 2791-2798 RB denotes readily
T22339 2799-2809 JJ denotes detectable
T22340 2817-2820 IN denotes for
T22341 2821-2823 NN denotes E2
T22342 2824-2826 IN denotes in
T22343 2827-2830 DT denotes the
T22344 2839-2844 NNS denotes cells
T22345 2831-2838 NN denotes control
T22346 2849-2856 JJ denotes present
T22347 2857-2859 IN denotes in
T22348 2860-2861 DT denotes a
T22349 2862-2869 NN denotes pattern
T22350 2870-2884 JJ denotes characteristic
T22351 2885-2887 IN denotes of
T22352 2888-2900 NNS denotes mitochondria
T22353 2900-2902 , denotes ,
T22354 2902-2905 DT denotes the
T22355 2918-2926 NN denotes location
T22356 2906-2917 JJ denotes subcellular
T22357 2927-2929 IN denotes of
T22358 2930-2935 NN denotes BCKDH
T22359 2935-2936 . denotes .
R1313 T5027 T5028 compound Gene,knockout
R1314 T5028 T5029 compound knockout,production
R1315 T5031 T5032 det A,subclone
R1316 T5032 T5035 nsubjpass subclone,obtained
R1317 T5033 T5032 amod genomic,subclone
R1318 T5034 T5032 compound DNA,subclone
R1319 T5036 T5032 prep of,subclone
R1320 T5037 T5038 nmod Strain,DNA
R1321 T5038 T5036 pobj DNA,of
R1322 T5039 T5040 nummod 129,SvJ
R1323 T5040 T5038 compound SvJ,DNA
R1324 T5041 T5040 punct /,SvJ
R1325 T5042 T5043 dep that,contained
R1326 T5043 T5032 relcl contained,subclone
R1327 T5044 T5045 det a,portion
R1328 T5045 T5043 dobj portion,contained
R1329 T5046 T5045 prep of,portion
R1330 T5047 T5048 nmod Exons,4
R1331 T5048 T5046 pobj 4,of
R1332 T5049 T5048 cc and,4
R1333 T5050 T5048 conj 5,4
R1334 T5051 T5048 prep of,4
R1335 T5052 T5053 det the,gene
R1336 T5053 T5051 pobj gene,of
R1337 T5054 T5053 compound E2,gene
R1338 T5055 T5053 cc and,gene
R1339 T5056 T5057 compound flanking,DNA
R1340 T5057 T5053 conj DNA,gene
R1341 T5058 T5035 auxpass was,obtained
R1342 T5059 T5035 prep from,obtained
R1343 T5060 T5061 det a,library
R1344 T5061 T5059 pobj library,from
R1345 T5062 T5063 compound P1,phage
R1346 T5063 T5061 compound phage,library
R1347 T5064 T5061 prep from,library
R1348 T5065 T5066 nmod Genome,Inc.
R1349 T5066 T5064 pobj Inc.,from
R1350 T5067 T5066 nmod Systems,Inc.
R1351 T5068 T5066 punct ", ",Inc.
R1352 T5069 T5066 punct ", ",Inc.
R1353 T5070 T5071 punct (,Library
R1354 T5071 T5066 poss Library,Inc.
R1355 T5072 T5073 compound St.,Louis
R1356 T5073 T5071 dep Louis,Library
R1357 T5074 T5073 punct ", ",Louis
R1358 T5075 T5073 npadvmod MO,Louis
R1359 T5076 T5071 punct ;,Library
R1360 T5077 T5071 punct ',Library
R1361 T5078 T5079 nummod 3,Hit
R1362 T5079 T5071 compound Hit,Library
R1363 T5080 T5079 punct -,Hit
R1364 T5081 T5082 compound Mouse,ES
R1365 T5082 T5071 compound ES,Library
R1366 T5083 T5071 dep ',Library
R1367 T5084 T5071 punct ),Library
R1368 T5085 T5035 punct .,obtained
R1369 T5087 T5088 det A,vector
R1370 T5088 T5096 nsubjpass vector,created
R1371 T5089 T5090 amod positive,negative
R1372 T5090 T5088 amod negative,vector
R1373 T5091 T5090 punct /,negative
R1374 T5092 T5093 compound replacement,type
R1375 T5093 T5088 compound type,vector
R1376 T5094 T5095 compound gene,targeting
R1377 T5095 T5088 compound targeting,vector
R1378 T5097 T5098 punct [,21
R1379 T5098 T5088 parataxis 21,vector
R1380 T5099 T5098 punct ],21
R1381 T5100 T5096 auxpass was,created
R1382 T5101 T5096 prep by,created
R1383 T5102 T5101 pcomp replacing,by
R1384 T5103 T5104 det a,fragment
R1385 T5104 T5102 dobj fragment,replacing
R1386 T5105 T5106 nummod 1.67,kb
R1387 T5106 T5104 compound kb,fragment
R1388 T5107 T5108 compound EcoRV,Smal
R1389 T5108 T5104 compound Smal,fragment
R1390 T5109 T5108 punct -,Smal
R1391 T5110 T5111 dep that,included
R1392 T5111 T5104 relcl included,fragment
R1393 T5112 T5113 det a,portion
R1394 T5113 T5111 dobj portion,included
R1395 T5114 T5113 prep of,portion
R1396 T5115 T5114 pobj Exon,of
R1397 T5116 T5115 nummod 4,Exon
R1398 T5117 T5113 cc and,portion
R1399 T5118 T5113 conj all,portion
R1400 T5119 T5118 prep of,all
R1401 T5120 T5119 pobj Exon,of
R1402 T5121 T5120 nummod 5,Exon
R1403 T5122 T5113 prep with,portion
R1404 T5123 T5124 det the,gene
R1405 T5124 T5122 pobj gene,with
R1406 T5125 T5124 compound PGKneo,gene
R1407 T5126 T5124 compound marker,gene
R1408 T5127 T5128 punct (,See
R1409 T5128 T5124 parataxis See,gene
R1410 T5129 T5130 compound Fig.,1A
R1411 T5130 T5128 dobj 1A,See
R1412 T5131 T5128 punct ),See
R1413 T5132 T5124 prep from,gene
R1414 T5133 T5134 det the,vector
R1415 T5134 T5132 pobj vector,from
R1416 T5135 T5134 compound pPNT,vector
R1417 T5136 T5137 punct [,22
R1418 T5137 T5111 parataxis 22,included
R1419 T5138 T5137 punct ],22
R1420 T5139 T5096 punct .,created
R1421 T5141 T5142 det The,construct
R1422 T5142 T5144 nsubjpass construct,linearized
R1423 T5143 T5142 compound targeting,construct
R1424 T5145 T5144 auxpass was,linearized
R1425 T5146 T5144 prep with,linearized
R1426 T5147 T5146 pobj NotI,with
R1427 T5148 T5144 cc and,linearized
R1428 T5149 T5144 conj electroporated,linearized
R1429 T5150 T5149 prep into,electroporated
R1430 T5151 T5152 compound R1,cells
R1431 T5152 T5150 pobj cells,into
R1432 T5153 T5152 compound ES,cells
R1433 T5154 T5155 punct [,23
R1434 T5155 T5152 parataxis 23,cells
R1435 T5156 T5155 punct ],23
R1436 T5157 T5149 prep under,electroporated
R1437 T5158 T5157 pobj conditions,under
R1438 T5159 T5158 acl described,conditions
R1439 T5160 T5159 advmod previously,described
R1440 T5161 T5162 punct [,24
R1441 T5162 T5159 parataxis 24,described
R1442 T5163 T5162 punct ],24
R1443 T5164 T5144 punct .,linearized
R1444 T5166 T5167 compound ES,cells
R1445 T5167 T5168 nsubjpass cells,selected
R1446 T5169 T5168 auxpass were,selected
R1447 T5170 T5168 prep with,selected
R1448 T5171 T5170 pobj G418,with
R1449 T5172 T5173 punct (,Inc.
R1450 T5173 T5171 parataxis Inc.,G418
R1451 T5174 T5175 nummod 300,μg
R1452 T5175 T5173 dep μg,Inc.
R1453 T5176 T5177 punct /,ml
R1454 T5177 T5175 prep ml,μg
R1455 T5178 T5173 punct ;,Inc.
R1456 T5179 T5173 compound Life,Inc.
R1457 T5180 T5173 compound Technologies,Inc.
R1458 T5181 T5173 punct ", ",Inc.
R1459 T5182 T5173 npadvmod Gaithersburg,Inc.
R1460 T5183 T5173 punct ", ",Inc.
R1461 T5184 T5173 npadvmod MD,Inc.
R1462 T5185 T5173 punct ),Inc.
R1463 T5186 T5171 cc and,G418
R1464 T5187 T5171 conj gancyclovir,G418
R1465 T5188 T5187 punct (,gancyclovir
R1466 T5189 T5190 nummod 2,μM
R1467 T5190 T5187 nmod μM,gancyclovir
R1468 T5191 T5187 punct ;,gancyclovir
R1469 T5192 T5187 nmod gift,gancyclovir
R1470 T5193 T5192 prep of,gift
R1471 T5194 T5193 pobj Syntex,of
R1472 T5195 T5194 punct ", ",Syntex
R1473 T5196 T5197 compound Palo,Alto
R1474 T5197 T5194 npadvmod Alto,Syntex
R1475 T5198 T5194 punct ", ",Syntex
R1476 T5199 T5194 npadvmod CA,Syntex
R1477 T5200 T5187 punct ),gancyclovir
R1478 T5201 T5168 punct .,selected
R1479 T5203 T5204 advmod Doubly,resistant
R1480 T5204 T5205 amod resistant,clones
R1481 T5205 T5206 nsubjpass clones,screened
R1482 T5207 T5206 auxpass were,screened
R1483 T5208 T5206 prep for,screened
R1484 T5209 T5210 compound gene,targeting
R1485 T5210 T5208 pobj targeting,for
R1486 T5211 T5206 prep by,screened
R1487 T5212 T5213 compound Southern,blot
R1488 T5213 T5214 compound blot,analysis
R1489 T5214 T5211 pobj analysis,by
R1490 T5215 T5214 prep of,analysis
R1491 T5216 T5217 nmod BglI,DNA
R1492 T5217 T5215 pobj DNA,of
R1493 T5218 T5217 amod digested,DNA
R1494 T5219 T5217 amod genomic,DNA
R1495 T5220 T5206 punct .,screened
R1496 T5222 T5223 nsubjpass Blots,hybridized
R1497 T5224 T5223 auxpass were,hybridized
R1498 T5225 T5223 prep with,hybridized
R1499 T5226 T5227 det an,probe
R1500 T5227 T5225 pobj probe,with
R1501 T5228 T5229 npadvmod Exon,specific
R1502 T5229 T5227 amod specific,probe
R1503 T5230 T5228 nummod 6,Exon
R1504 T5231 T5232 dep that,was
R1505 T5232 T5227 relcl was,probe
R1506 T5233 T5232 acomp external,was
R1507 T5234 T5233 prep to,external
R1508 T5235 T5236 det the,construct
R1509 T5236 T5234 pobj construct,to
R1510 T5237 T5238 compound gene,targeting
R1511 T5238 T5236 compound targeting,construct
R1512 T5239 T5223 punct .,hybridized
R1513 T5241 T5242 advmod Correctly,targeted
R1514 T5242 T5243 amod targeted,cells
R1515 T5243 T5245 nsubjpass cells,injected
R1516 T5244 T5243 compound ES,cells
R1517 T5246 T5245 auxpass were,injected
R1518 T5247 T5245 prep into,injected
R1519 T5248 T5249 compound C57BL,6J
R1520 T5249 T5251 compound 6J,blastocysts
R1521 T5250 T5249 punct /,6J
R1522 T5251 T5247 pobj blastocysts,into
R1523 T5252 T5253 aux to,produce
R1524 T5253 T5245 advcl produce,injected
R1525 T5254 T5255 amod chimeric,mice
R1526 T5255 T5253 dobj mice,produce
R1527 T5256 T5253 advcl using,produce
R1528 T5257 T5258 amod standard,procedures
R1529 T5258 T5256 dobj procedures,using
R1530 T5259 T5245 punct .,injected
R1531 T5261 T5262 amod Heterozygous,offspring
R1532 T5262 T5263 nsubjpass offspring,intercrossed
R1533 T5264 T5262 prep from,offspring
R1534 T5265 T5266 npadvmod germline,competent
R1535 T5266 T5267 amod competent,chimeras
R1536 T5267 T5264 pobj chimeras,from
R1537 T5268 T5263 auxpass were,intercrossed
R1538 T5269 T5270 aux to,produce
R1539 T5270 T5263 advcl produce,intercrossed
R1540 T5271 T5272 amod wild,type
R1541 T5272 T5273 nmod type,knockout
R1542 T5273 T5288 nmod knockout,mice
R1543 T5274 T5275 punct (,+
R1544 T5275 T5272 punct +,type
R1545 T5276 T5275 punct +,+
R1546 T5277 T5275 punct /,+
R1547 T5278 T5275 punct ),+
R1548 T5279 T5272 punct ", ",type
R1549 T5280 T5272 conj heterozygous,type
R1550 T5281 T5282 punct (,-
R1551 T5282 T5280 punct -,heterozygous
R1552 T5283 T5282 punct +,-
R1553 T5284 T5282 punct /,-
R1554 T5285 T5282 punct ),-
R1555 T5286 T5280 cc and,heterozygous
R1556 T5287 T5280 conj homozygous,heterozygous
R1557 T5288 T5270 dobj mice,produce
R1558 T5289 T5290 punct (,-
R1559 T5290 T5273 punct -,knockout
R1560 T5291 T5290 punct -,-
R1561 T5292 T5290 punct /,-
R1562 T5293 T5290 punct ),-
R1563 T5294 T5263 punct .,intercrossed
R1564 T5296 T5297 prep At,used
R1565 T5298 T5299 det all,generations
R1566 T5299 T5296 pobj generations,At
R1567 T5300 T5297 punct ", ",used
R1568 T5301 T5302 punct +,-
R1569 T5302 T5304 punct -,pairs
R1570 T5303 T5302 punct /,-
R1571 T5304 T5297 nsubjpass pairs,used
R1572 T5305 T5304 amod breeding,pairs
R1573 T5306 T5297 auxpass were,used
R1574 T5307 T5297 punct .,used
R1575 T5309 T5310 nsubj Results,are
R1576 T5311 T5309 acl presented,Results
R1577 T5312 T5311 advmod here,presented
R1578 T5313 T5310 prep from,are
R1579 T5314 T5313 pobj mice,from
R1580 T5315 T5314 acl derived,mice
R1581 T5316 T5315 prep from,derived
R1582 T5317 T5318 det the,generations
R1583 T5318 T5316 pobj generations,from
R1584 T5319 T5318 nmod F2,generations
R1585 T5320 T5319 punct +,F2
R1586 T5321 T5310 punct .,are
R1587 T5323 T5324 det All,animals
R1588 T5324 T5325 nsubj animals,were
R1589 T5326 T5325 prep of,were
R1590 T5327 T5328 det a,background
R1591 T5328 T5326 pobj background,of
R1592 T5329 T5328 amod mixed,background
R1593 T5330 T5331 nmod C57BL,6J
R1594 T5331 T5328 nmod 6J,background
R1595 T5332 T5331 punct /,6J
R1596 T5333 T5331 punct ×,6J
R1597 T5334 T5335 compound Strain,SvJ
R1598 T5335 T5331 appos SvJ,6J
R1599 T5336 T5335 compound 129Sv,SvJ
R1600 T5337 T5335 punct /,SvJ
R1601 T5338 T5328 amod genetic,background
R1602 T5339 T5325 punct .,were
R6272 T22055 T22056 compound E2,production
R6273 T22057 T22058 compound gene,knockout
R6274 T22058 T22056 compound knockout,production
R6275 T22059 T22056 compound mouse,production
R6276 T22060 T22056 punct .,production
R6277 T22062 T22063 meta A,strategy
R6278 T22064 T22063 punct ", ",strategy
R6279 T22065 T22066 compound Gene,targeting
R6280 T22066 T22063 compound targeting,strategy
R6281 T22067 T22063 acl used,strategy
R6282 T22068 T22067 prep for,used
R6283 T22069 T22068 pcomp targeting,for
R6284 T22070 T22071 det the,locus
R6285 T22071 T22069 dobj locus,targeting
R6286 T22072 T22071 compound E2,locus
R6287 T22073 T22067 prep in,used
R6288 T22074 T22075 compound mouse,cells
R6289 T22075 T22073 pobj cells,in
R6290 T22076 T22075 compound ES,cells
R6291 T22077 T22063 punct .,strategy
R6292 T22079 T22080 det The,construct
R6293 T22080 T22082 nsubjpass construct,designed
R6294 T22081 T22080 compound targeting,construct
R6295 T22083 T22082 auxpass was,designed
R6296 T22084 T22085 aux to,delete
R6297 T22085 T22082 advcl delete,designed
R6298 T22086 T22087 nummod 1.67,kb
R6299 T22087 T22085 dobj kb,delete
R6300 T22088 T22087 prep of,kb
R6301 T22089 T22088 pobj sequence,of
R6302 T22090 T22085 prep between,delete
R6303 T22091 T22092 det an,site
R6304 T22092 T22090 pobj site,between
R6305 T22093 T22092 compound EcoRV,site
R6306 T22094 T22092 prep in,site
R6307 T22095 T22094 pobj Exon,in
R6308 T22096 T22095 nummod 4,Exon
R6309 T22097 T22092 cc and,site
R6310 T22098 T22099 det a,site
R6311 T22099 T22092 conj site,site
R6312 T22100 T22099 compound Smal,site
R6313 T22101 T22099 prep in,site
R6314 T22102 T22101 pobj intron,in
R6315 T22103 T22102 nummod 5,intron
R6316 T22104 T22082 punct .,designed
R6317 T22106 T22107 det The,gene
R6318 T22107 T22111 nsubj gene,contains
R6319 T22108 T22109 amod wild,type
R6320 T22109 T22107 compound type,gene
R6321 T22110 T22107 compound E2,gene
R6322 T22112 T22113 det an,fragment
R6323 T22113 T22111 dobj fragment,contains
R6324 T22114 T22115 punct ~,16
R6325 T22115 T22116 nummod 16,kb
R6326 T22116 T22113 compound kb,fragment
R6327 T22117 T22113 compound BglI,fragment
R6328 T22118 T22113 compound restriction,fragment
R6329 T22119 T22120 dep that,hybridizes
R6330 T22120 T22113 relcl hybridizes,fragment
R6331 T22121 T22120 prep to,hybridizes
R6332 T22122 T22123 det the,probe
R6333 T22123 T22121 pobj probe,to
R6334 T22124 T22125 npadvmod Exon,specific
R6335 T22125 T22123 amod specific,probe
R6336 T22126 T22124 nummod 6,Exon
R6337 T22127 T22111 punct .,contains
R6338 T22129 T22130 det A,locus
R6339 T22130 T22134 nsubj locus,harbors
R6340 T22131 T22132 advmod correctly,targeted
R6341 T22132 T22130 amod targeted,locus
R6342 T22133 T22130 compound E2,locus
R6343 T22135 T22136 det an,fragment
R6344 T22136 T22134 dobj fragment,harbors
R6345 T22137 T22138 punct ~,11
R6346 T22138 T22139 nummod 11,kb
R6347 T22139 T22136 compound kb,fragment
R6348 T22140 T22136 compound BglI,fragment
R6349 T22141 T22136 compound restriction,fragment
R6350 T22142 T22143 dep that,hybridizes
R6351 T22143 T22136 relcl hybridizes,fragment
R6352 T22144 T22143 prep to,hybridizes
R6353 T22145 T22146 det the,probe
R6354 T22146 T22144 pobj probe,to
R6355 T22147 T22146 amod same,probe
R6356 T22148 T22134 punct .,harbors
R6357 T22151 T22152 mark that,detect
R6358 T22152 T22150 ccomp detect,Note
R6359 T22153 T22154 det the,probe
R6360 T22154 T22152 nsubj probe,detect
R6361 T22155 T22152 aux will,detect
R6362 T22156 T22152 neg not,detect
R6363 T22157 T22158 amod random,integration
R6364 T22158 T22152 dobj integration,detect
R6365 T22159 T22158 prep of,integration
R6366 T22160 T22161 det the,vector
R6367 T22161 T22159 pobj vector,of
R6368 T22162 T22161 compound targeting,vector
R6369 T22163 T22164 mark because,is
R6370 T22164 T22152 advcl is,detect
R6371 T22165 T22164 nsubj it,is
R6372 T22166 T22164 acomp external,is
R6373 T22167 T22166 prep to,external
R6374 T22168 T22169 det the,vector
R6375 T22169 T22167 pobj vector,to
R6376 T22170 T22169 compound targeting,vector
R6377 T22171 T22150 punct .,Note
R6378 T22173 T22174 meta B,analysis
R6379 T22175 T22174 punct ", ",analysis
R6380 T22176 T22177 compound Southern,blot
R6381 T22177 T22174 compound blot,analysis
R6382 T22178 T22174 prep of,analysis
R6383 T22179 T22180 npadvmod Bgll,digested
R6384 T22180 T22181 amod digested,DNA
R6385 T22181 T22178 pobj DNA,of
R6386 T22182 T22181 amod genomic,DNA
R6387 T22183 T22181 acl derived,DNA
R6388 T22184 T22183 prep from,derived
R6389 T22185 T22186 det the,line
R6390 T22186 T22184 pobj line,from
R6391 T22187 T22186 amod parental,line
R6392 T22188 T22189 amod wild,type
R6393 T22189 T22186 compound type,line
R6394 T22190 T22191 compound ES,cell
R6395 T22191 T22186 compound cell,line
R6396 T22192 T22193 punct (,R1
R6397 T22193 T22186 parataxis R1,line
R6398 T22194 T22193 punct ),R1
R6399 T22195 T22186 punct ", ",line
R6400 T22196 T22197 det a,line
R6401 T22197 T22186 appos line,line
R6402 T22198 T22197 amod heterozygous,line
R6403 T22199 T22197 amod targeted,line
R6404 T22200 T22201 compound ES,cell
R6405 T22201 T22197 compound cell,line
R6406 T22202 T22203 punct (,362
R6407 T22203 T22197 parataxis 362,line
R6408 T22204 T22203 punct ),362
R6409 T22205 T22184 punct ", ",from
R6410 T22206 T22184 cc and,from
R6411 T22207 T22184 conj from,from
R6412 T22208 T22209 amod wild,type
R6413 T22209 T22210 nmod type,knockout
R6414 T22210 T22225 nmod knockout,mice
R6415 T22211 T22212 punct (,+
R6416 T22212 T22209 punct +,type
R6417 T22213 T22212 punct +,+
R6418 T22214 T22212 punct /,+
R6419 T22215 T22212 punct ),+
R6420 T22216 T22209 punct ", ",type
R6421 T22217 T22209 conj heterozygous,type
R6422 T22218 T22219 punct (,-
R6423 T22219 T22217 punct -,heterozygous
R6424 T22220 T22219 punct +,-
R6425 T22221 T22219 punct /,-
R6426 T22222 T22219 punct ),-
R6427 T22223 T22217 cc and,heterozygous
R6428 T22224 T22217 conj homozygous,heterozygous
R6429 T22225 T22207 pobj mice,from
R6430 T22226 T22227 punct (,-
R6431 T22227 T22210 punct -,knockout
R6432 T22228 T22227 punct -,-
R6433 T22229 T22227 punct /,-
R6434 T22230 T22227 punct ),-
R6435 T22231 T22174 punct .,analysis
R6436 T22233 T22234 det The,blot
R6437 T22234 T22235 nsubjpass blot,hybridized
R6438 T22236 T22235 auxpass was,hybridized
R6439 T22237 T22235 prep with,hybridized
R6440 T22238 T22239 det an,probe
R6441 T22239 T22237 pobj probe,with
R6442 T22240 T22241 npadvmod Exon,specific
R6443 T22241 T22239 amod specific,probe
R6444 T22242 T22240 nummod 6,Exon
R6445 T22243 T22235 punct .,hybridized
R6446 T22245 T22246 meta C,analysis
R6447 T22247 T22246 punct ", ",analysis
R6448 T22248 T22246 amod Immunohistochemical,analysis
R6449 T22249 T22246 prep of,analysis
R6450 T22250 T22251 amod fresh,frozen
R6451 T22251 T22252 amod frozen,sections
R6452 T22252 T22249 pobj sections,of
R6453 T22253 T22252 compound liver,sections
R6454 T22254 T22252 prep from,sections
R6455 T22255 T22256 nmod control,knockout
R6456 T22256 T22264 nmod knockout,pups
R6457 T22257 T22258 punct (,+
R6458 T22258 T22255 punct +,control
R6459 T22259 T22258 punct +,+
R6460 T22260 T22258 punct /,+
R6461 T22261 T22258 punct ),+
R6462 T22262 T22255 cc and,control
R6463 T22263 T22255 conj E2,control
R6464 T22264 T22254 pobj pups,from
R6465 T22265 T22266 punct (,-
R6466 T22266 T22256 punct -,knockout
R6467 T22267 T22266 punct -,-
R6468 T22268 T22266 punct /,-
R6469 T22269 T22266 punct ),-
R6470 T22270 T22271 amod postnatal,day
R6471 T22271 T22264 nmod day,pups
R6472 T22272 T22271 nummod 1,day
R6473 T22273 T22264 compound mouse,pups
R6474 T22274 T22246 punct .,analysis
R6475 T22276 T22277 nsubjpass Sections,stained
R6476 T22278 T22277 auxpass were,stained
R6477 T22279 T22277 prep for,stained
R6478 T22280 T22279 pobj E2,for
R6479 T22281 T22277 advcl using,stained
R6480 T22282 T22283 det an,antibody
R6481 T22283 T22281 dobj antibody,using
R6482 T22284 T22285 npadvmod E2,specific
R6483 T22285 T22283 amod specific,antibody
R6484 T22286 T22287 punct (,green
R6485 T22287 T22283 parataxis green,antibody
R6486 T22288 T22287 punct ),green
R6487 T22289 T22283 cc and,antibody
R6488 T22290 T22291 det a,stain
R6489 T22291 T22283 conj stain,antibody
R6490 T22292 T22291 amod nuclear,stain
R6491 T22293 T22294 punct (,blue
R6492 T22294 T22291 parataxis blue,stain
R6493 T22295 T22294 punct ),blue
R6494 T22296 T22277 punct .,stained
R6495 T22299 T22300 det the,absence
R6496 T22300 T22298 dobj absence,Note
R6497 T22301 T22300 amod complete,absence
R6498 T22302 T22300 prep of,absence
R6499 T22303 T22304 compound E2,immunoreactivity
R6500 T22304 T22302 pobj immunoreactivity,of
R6501 T22305 T22298 prep in,Note
R6502 T22306 T22307 det the,section
R6503 T22307 T22305 pobj section,in
R6504 T22308 T22307 prep from,section
R6505 T22309 T22310 det the,mouse
R6506 T22310 T22308 pobj mouse,from
R6507 T22311 T22310 compound knockout,mouse
R6508 T22312 T22298 punct .,Note
R6509 T22314 T22315 meta D,observed
R6510 T22316 T22315 punct ", ",observed
R6511 T22317 T22318 amod Similar,results
R6512 T22318 T22315 nsubjpass results,observed
R6513 T22319 T22315 auxpass were,observed
R6514 T22320 T22315 prep upon,observed
R6515 T22321 T22322 amod immunohistochemical,analysis
R6516 T22322 T22320 pobj analysis,upon
R6517 T22323 T22322 prep of,analysis
R6518 T22324 T22325 amod primary,fibroblasts
R6519 T22325 T22323 pobj fibroblasts,of
R6520 T22326 T22325 nmod mouse,fibroblasts
R6521 T22327 T22325 amod embryonic,fibroblasts
R6522 T22328 T22325 punct (,fibroblasts
R6523 T22329 T22325 appos MEFs,fibroblasts
R6524 T22330 T22315 punct ),observed
R6525 T22331 T22315 punct .,observed
R6526 T22334 T22335 mark that,was
R6527 T22335 T22333 ccomp was,Note
R6528 T22336 T22337 det the,signal
R6529 T22337 T22335 nsubj signal,was
R6530 T22338 T22339 advmod readily,detectable
R6531 T22339 T22337 amod detectable,signal
R6532 T22340 T22337 prep for,signal
R6533 T22341 T22340 pobj E2,for
R6534 T22342 T22337 prep in,signal
R6535 T22343 T22344 det the,cells
R6536 T22344 T22342 pobj cells,in
R6537 T22345 T22344 compound control,cells
R6538 T22346 T22335 acomp present,was
R6539 T22347 T22346 prep in,present
R6540 T22348 T22349 det a,pattern
R6541 T22349 T22347 pobj pattern,in
R6542 T22350 T22349 amod characteristic,pattern
R6543 T22351 T22350 prep of,characteristic
R6544 T22352 T22351 pobj mitochondria,of
R6545 T22353 T22352 punct ", ",mitochondria
R6546 T22354 T22355 det the,location
R6547 T22355 T22352 appos location,mitochondria
R6548 T22356 T22355 amod subcellular,location
R6549 T22357 T22355 prep of,location
R6550 T22358 T22357 pobj BCKDH,of
R6551 T22359 T22333 punct .,Note

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T4968 0-4 SO_EXT:0000704 denotes Gene
T4969 27-38 SO_EXT:genomic_DNA denotes genomic DNA
T4970 35-38 CHEBI_SO_EXT:DNA denotes DNA
T4971 66-69 CHEBI_SO_EXT:DNA denotes DNA
T4972 87-103 SO_EXT:0000852 denotes portion of Exons
T4973 119-121 PR_EXT:000006300 denotes E2
T4974 122-126 SO_EXT:0000704 denotes gene
T4975 131-139 SO:0000357 denotes flanking
T4976 140-143 CHEBI_SO_EXT:DNA denotes DNA
T4977 164-172 NCBITaxon:10678 denotes P1 phage
T4978 186-192 SO_EXT:0001026 denotes Genome
T4979 231-236 NCBITaxon:10088 denotes Mouse
T4980 237-239 CL:0002322 denotes ES
T4981 271-282 SO_EXT:sequence_substitution_entity_or_process denotes replacement
T4982 288-292 SO_EXT:0000704 denotes gene
T4983 293-309 SO_EXT:0001644 denotes targeting vector
T4984 330-339 SO_EXT:sequence_substitution_process denotes replacing
T4985 348-349 CHEBI_SO_EXT:base denotes b
T4986 386-401 SO_EXT:0000852 denotes portion of Exon
T4987 415-419 SO_EXT:0000147 denotes Exon
T4988 438-444 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T4989 445-449 SO_EXT:0000704 denotes gene
T4990 478-484 SO_EXT:0000440 denotes vector
T4991 505-514 SO_EXT:engineered_biological_sequence denotes construct
T4992 519-529 SO_EXT:sequence_linearization denotes linearized
T4993 567-575 CL:0002322 denotes ES cells
T4994 570-575 CL_GO_EXT:cell denotes cells
T4995 625-633 CL:0002322 denotes ES cells
T4996 628-633 CL_GO_EXT:cell denotes cells
T4997 653-657 CHEBI:42768 denotes G418
T4998 670-674 UBERON:0000104 denotes Life
T4999 716-727 CHEBI:465284 denotes gancyclovir
T5000 784-790 SO_EXT:sequence_cloned_entity denotes clones
T5001 809-813 SO_EXT:0000704 denotes gene
T5002 867-878 SO_EXT:genomic_DNA denotes genomic DNA
T5003 875-878 CHEBI_SO_EXT:DNA denotes DNA
T5004 891-901 GO:0097617 denotes hybridized
T5005 910-914 SO_EXT:0000147 denotes Exon
T5006 926-931 CHEBI_SO_EXT:molecular_probe denotes probe
T5007 957-961 SO_EXT:0000704 denotes gene
T5008 972-981 SO_EXT:engineered_biological_sequence denotes construct
T5009 1002-1010 CL:0002322 denotes ES cells
T5010 1005-1010 CL_GO_EXT:cell denotes cells
T5011 1039-1050 UBERON:0000358 denotes blastocysts
T5012 1071-1075 NCBITaxon:10088 denotes mice
T5013 1188-1197 SO_EXT:wild_type_entity_or_quality denotes wild type
T5014 1199-1200 SO_EXT:normal_or_wild_type_or_present denotes +
T5015 1201-1202 SO_EXT:normal_or_wild_type_or_present denotes +
T5016 1219-1220 SO_EXT:normal_or_wild_type_or_present denotes +
T5017 1221-1222 SO_EXT:sequence_nullness_or_absence denotes -
T5018 1249-1250 SO_EXT:sequence_nullness_or_absence denotes -
T5019 1251-1252 SO_EXT:sequence_nullness_or_absence denotes -
T5020 1254-1258 NCBITaxon:10088 denotes mice
T5021 1280-1281 SO_EXT:normal_or_wild_type_or_present denotes +
T5022 1282-1283 SO_EXT:sequence_nullness_or_absence denotes -
T5023 1284-1292 GO_EXT:breeding denotes breeding
T5024 1342-1346 NCBITaxon:10088 denotes mice
T5025 1385-1392 NCBITaxon:33208 denotes animals
T5026 1437-1444 SO_EXT:0000704 denotes genetic
T21984 1467-1469 PR_EXT:000006300 denotes E2
T21985 1470-1474 SO_EXT:0000704 denotes gene
T21986 1484-1489 NCBITaxon:10088 denotes mouse
T21987 1505-1509 SO_EXT:0000704 denotes Gene
T21988 1552-1554 PR_EXT:000006300 denotes E2
T21989 1564-1569 NCBITaxon:10088 denotes mouse
T21990 1570-1578 CL:0002322 denotes ES cells
T21991 1573-1578 CL_GO_EXT:cell denotes cells
T21992 1594-1603 SO_EXT:engineered_biological_sequence denotes construct
T21993 1620-1626 SO_EXT:sequence_deletion_process denotes delete
T21994 1633-1634 CHEBI_SO_EXT:base denotes b
T21995 1638-1646 SO_EXT:biological_sequence denotes sequence
T21996 1664-1676 SO_EXT:0000852 denotes site in Exon
T21997 1698-1704 SO_EXT:0000188 denotes intron
T21998 1712-1721 SO_EXT:wild_type_entity_or_quality denotes wild type
T21999 1722-1724 PR_EXT:000006300 denotes E2
T22000 1725-1729 SO_EXT:0000704 denotes gene
T22001 1747-1748 CHEBI_SO_EXT:base denotes b
T22002 1754-1774 SO_EXT:0000412 denotes restriction fragment
T22003 1780-1790 GO:0097617 denotes hybridizes
T22004 1798-1802 SO_EXT:0000147 denotes Exon
T22005 1814-1819 CHEBI_SO_EXT:molecular_probe denotes probe
T22006 1842-1844 PR_EXT:000006300 denotes E2
T22007 1867-1868 CHEBI_SO_EXT:base denotes b
T22008 1874-1894 SO_EXT:0000412 denotes restriction fragment
T22009 1900-1910 GO:0097617 denotes hybridizes
T22010 1923-1928 CHEBI_SO_EXT:molecular_probe denotes probe
T22011 1944-1949 CHEBI_SO_EXT:molecular_probe denotes probe
T22012 1992-2008 SO_EXT:0001644 denotes targeting vector
T22013 2039-2055 SO_EXT:0001644 denotes targeting vector
T22014 2100-2111 SO_EXT:genomic_DNA denotes genomic DNA
T22015 2108-2111 CHEBI_SO_EXT:DNA denotes DNA
T22016 2138-2147 SO_EXT:wild_type_entity_or_quality denotes wild type
T22017 2148-2155 CL:0002322 denotes ES cell
T22018 2151-2155 CL_GO_EXT:cell denotes cell
T22019 2191-2198 CL:0002322 denotes ES cell
T22020 2194-2198 CL_GO_EXT:cell denotes cell
T22021 2220-2229 SO_EXT:wild_type_entity_or_quality denotes wild type
T22022 2231-2232 SO_EXT:normal_or_wild_type_or_present denotes +
T22023 2233-2234 SO_EXT:normal_or_wild_type_or_present denotes +
T22024 2251-2252 SO_EXT:normal_or_wild_type_or_present denotes +
T22025 2253-2254 SO_EXT:sequence_nullness_or_absence denotes -
T22026 2281-2282 SO_EXT:sequence_nullness_or_absence denotes -
T22027 2283-2284 SO_EXT:sequence_nullness_or_absence denotes -
T22028 2286-2290 NCBITaxon:10088 denotes mice
T22029 2305-2315 GO:0097617 denotes hybridized
T22030 2324-2328 SO_EXT:0000147 denotes Exon
T22031 2340-2345 CHEBI_SO_EXT:molecular_probe denotes probe
T22032 2395-2400 UBERON:0002107 denotes liver
T22033 2424-2425 SO_EXT:normal_or_wild_type_or_present denotes +
T22034 2426-2427 SO_EXT:normal_or_wild_type_or_present denotes +
T22035 2433-2435 PR_EXT:000006300 denotes E2
T22036 2446-2447 SO_EXT:sequence_nullness_or_absence denotes -
T22037 2448-2449 SO_EXT:sequence_nullness_or_absence denotes -
T22038 2455-2460 GO:0007567 denotes natal
T22039 2467-2472 NCBITaxon:10088 denotes mouse
T22040 2473-2477 NCBITaxon_UBERON_EXT:pup denotes pups
T22041 2505-2507 PR_EXT:000006300 denotes E2
T22042 2517-2519 PR_EXT:000006300 denotes E2
T22043 2529-2537 GO:0042571 denotes antibody
T22044 2552-2559 GO:0005634 denotes nuclear
T22045 2603-2605 PR_EXT:000006300 denotes E2
T22046 2656-2661 NCBITaxon:10088 denotes mouse
T22047 2741-2746 NCBITaxon:10088 denotes mouse
T22048 2747-2756 UBERON:0000922 denotes embryonic
T22049 2757-2768 CL:0000057 denotes fibroblasts
T22050 2821-2823 PR_EXT:000006300 denotes E2
T22051 2839-2844 CL_GO_EXT:cell denotes cells
T22052 2888-2900 GO:0005739 denotes mitochondria
T22053 2906-2917 GO_UBERON_EXT:cellular_component_or_cell_part denotes subcellular
T22054 2909-2917 CL_GO_EXT:cell denotes cellular

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T4935 0-4 SO:0000704 denotes Gene
T4936 27-34 SO:0001026 denotes genomic
T4937 87-103 SO:0000852 denotes portion of Exons
T4938 119-121 PR:000006300 denotes E2
T4939 122-126 SO:0000704 denotes gene
T4940 131-139 SO:0000357 denotes flanking
T4941 164-172 NCBITaxon:10678 denotes P1 phage
T4942 186-192 SO:0001026 denotes Genome
T4943 231-236 NCBITaxon:10088 denotes Mouse
T4944 237-239 CL:0002322 denotes ES
T4945 288-292 SO:0000704 denotes gene
T4946 293-309 SO:0001644 denotes targeting vector
T4947 386-401 SO:0000852 denotes portion of Exon
T4948 415-419 SO:0000147 denotes Exon
T4949 445-449 SO:0000704 denotes gene
T4950 478-484 SO:0000440 denotes vector
T4951 567-575 CL:0002322 denotes ES cells
T4952 625-633 CL:0002322 denotes ES cells
T4953 653-657 CHEBI:42768 denotes G418
T4954 670-674 UBERON:0000104 denotes Life
T4955 716-727 CHEBI:465284 denotes gancyclovir
T4956 809-813 SO:0000704 denotes gene
T4957 867-874 SO:0001026 denotes genomic
T4958 891-901 GO:0097617 denotes hybridized
T4959 910-914 SO:0000147 denotes Exon
T4960 957-961 SO:0000704 denotes gene
T4961 1002-1010 CL:0002322 denotes ES cells
T4962 1039-1050 UBERON:0000358 denotes blastocysts
T4963 1071-1075 NCBITaxon:10088 denotes mice
T4964 1254-1258 NCBITaxon:10088 denotes mice
T4965 1342-1346 NCBITaxon:10088 denotes mice
T4966 1385-1392 NCBITaxon:33208 denotes animals
T4967 1437-1444 SO:0000704 denotes genetic
T21944 1467-1469 PR:000006300 denotes E2
T21945 1470-1474 SO:0000704 denotes gene
T21946 1484-1489 NCBITaxon:10088 denotes mouse
T21947 1505-1509 SO:0000704 denotes Gene
T21948 1552-1554 PR:000006300 denotes E2
T21949 1564-1569 NCBITaxon:10088 denotes mouse
T21950 1570-1578 CL:0002322 denotes ES cells
T21951 1664-1676 SO:0000852 denotes site in Exon
T21952 1698-1704 SO:0000188 denotes intron
T21953 1722-1724 PR:000006300 denotes E2
T21954 1725-1729 SO:0000704 denotes gene
T21955 1754-1774 SO:0000412 denotes restriction fragment
T21956 1780-1790 GO:0097617 denotes hybridizes
T21957 1798-1802 SO:0000147 denotes Exon
T21958 1842-1844 PR:000006300 denotes E2
T21959 1874-1894 SO:0000412 denotes restriction fragment
T21960 1900-1910 GO:0097617 denotes hybridizes
T21961 1992-2008 SO:0001644 denotes targeting vector
T21962 2039-2055 SO:0001644 denotes targeting vector
T21963 2100-2107 SO:0001026 denotes genomic
T21964 2148-2155 CL:0002322 denotes ES cell
T21965 2191-2198 CL:0002322 denotes ES cell
T21966 2286-2290 NCBITaxon:10088 denotes mice
T21967 2305-2315 GO:0097617 denotes hybridized
T21968 2324-2328 SO:0000147 denotes Exon
T21969 2395-2400 UBERON:0002107 denotes liver
T21970 2433-2435 PR:000006300 denotes E2
T21971 2455-2460 GO:0007567 denotes natal
T21972 2467-2472 NCBITaxon:10088 denotes mouse
T21973 2505-2507 PR:000006300 denotes E2
T21974 2517-2519 PR:000006300 denotes E2
T21975 2529-2537 GO:0042571 denotes antibody
T21976 2552-2559 GO:0005634 denotes nuclear
T21977 2603-2605 PR:000006300 denotes E2
T21978 2656-2661 NCBITaxon:10088 denotes mouse
T21979 2741-2746 NCBITaxon:10088 denotes mouse
T21980 2747-2756 UBERON:0000922 denotes embryonic
T21981 2757-2768 CL:0000057 denotes fibroblasts
T21982 2821-2823 PR:000006300 denotes E2
T21983 2888-2900 GO:0005739 denotes mitochondria