> top > docs > PMC:1448208 > spans > 7185-16333 > annotations

PMC:1448208 / 7185-16333 JSONTXT

Annnotations TAB JSON ListView MergeView

craft-sa-dev

Id Subject Object Predicate Lexical cue
T4359 8-11 DT denotes All
T4360 12-19 NNS denotes studies
T4361 43-51 VBN denotes reviewed
T4362 20-29 VBG denotes involving
T4363 30-37 NNS denotes animals
T4364 38-42 VBD denotes were
T4365 52-55 CC denotes and
T4366 56-64 VBN denotes approved
T4367 65-67 IN denotes by
T4368 68-71 DT denotes the
T4369 72-82 NNP denotes University
T4370 83-85 IN denotes of
T4371 86-96 NNP denotes Pittsburgh
T4372 133-142 NNP denotes Committee
T4373 96-98 POS denotes 's
T4374 99-112 NNP denotes Institutional
T4375 113-119 NNP denotes Animal
T4376 120-124 NNP denotes Care
T4377 125-128 CC denotes and
T4378 129-132 NNP denotes Use
T4379 142-143 . denotes .
T5027 145-149 NN denotes Gene
T5028 150-158 NN denotes knockout
T5029 159-169 NN denotes production
T5030 169-395 sentence denotes A genomic DNA subclone of Strain 129/SvJ DNA that contained a portion of Exons 4 and 5 of the E2 gene and flanking DNA was obtained from a P1 phage library from Genome Systems, Inc., (St. Louis, MO; '3-Hit Mouse ES Library').
T5031 170-171 DT denotes A
T5032 184-192 NN denotes subclone
T5033 172-179 JJ denotes genomic
T5034 180-183 NN denotes DNA
T5035 293-301 VBN denotes obtained
T5036 193-195 IN denotes of
T5037 196-202 NN denotes Strain
T5038 211-214 NN denotes DNA
T5039 203-206 CD denotes 129
T5040 207-210 NN denotes SvJ
T5041 206-207 HYPH denotes /
T5042 215-219 WDT denotes that
T5043 220-229 VBD denotes contained
T5044 230-231 DT denotes a
T5045 232-239 NN denotes portion
T5046 240-242 IN denotes of
T5047 243-248 NNS denotes Exons
T5048 249-250 CD denotes 4
T5049 251-254 CC denotes and
T5050 255-256 CD denotes 5
T5051 257-259 IN denotes of
T5052 260-263 DT denotes the
T5053 267-271 NN denotes gene
T5054 264-266 NN denotes E2
T5055 272-275 CC denotes and
T5056 276-284 NN denotes flanking
T5057 285-288 NN denotes DNA
T5058 289-292 VBD denotes was
T5059 302-306 IN denotes from
T5060 307-308 DT denotes a
T5061 318-325 NN denotes library
T5062 309-311 NN denotes P1
T5063 312-317 NN denotes phage
T5064 326-330 IN denotes from
T5065 331-337 NNP denotes Genome
T5066 347-351 NNP denotes Inc.
T5067 338-345 NNP denotes Systems
T5068 345-347 , denotes ,
T5069 351-353 , denotes ,
T5070 353-354 -LRB- denotes (
T5071 385-392 NNP denotes Library
T5072 354-357 NNP denotes St.
T5073 358-363 NNP denotes Louis
T5074 363-365 , denotes ,
T5075 365-367 NNP denotes MO
T5076 367-368 : denotes ;
T5077 369-370 SYM denotes '
T5078 370-371 CD denotes 3
T5079 372-375 NN denotes Hit
T5080 371-372 HYPH denotes -
T5081 376-381 NNP denotes Mouse
T5082 382-384 NNP denotes ES
T5083 392-393 POS denotes '
T5084 393-394 -RRB- denotes )
T5085 394-395 . denotes .
T5086 395-635 sentence denotes A positive/negative replacement type gene targeting vector [21] was created by replacing a 1.67 kb EcoRV-Smal fragment that included a portion of Exon 4 and all of Exon 5 with the PGKneo marker gene (See Fig. 1A) from the pPNT vector [22].
T5087 396-397 DT denotes A
T5088 448-454 NN denotes vector
T5089 398-406 JJ denotes positive
T5090 407-415 JJ denotes negative
T5091 406-407 HYPH denotes /
T5092 416-427 NN denotes replacement
T5093 428-432 NN denotes type
T5094 433-437 NN denotes gene
T5095 438-447 NN denotes targeting
T5096 464-471 VBN denotes created
T5097 455-456 -LRB- denotes [
T5098 456-458 CD denotes 21
T5099 458-459 -RRB- denotes ]
T5100 460-463 VBD denotes was
T5101 472-474 IN denotes by
T5102 475-484 VBG denotes replacing
T5103 485-486 DT denotes a
T5104 506-514 NN denotes fragment
T5105 487-491 CD denotes 1.67
T5106 492-494 NN denotes kb
T5107 495-500 NN denotes EcoRV
T5108 501-505 NN denotes Smal
T5109 500-501 HYPH denotes -
T5110 515-519 WDT denotes that
T5111 520-528 VBD denotes included
T5112 529-530 DT denotes a
T5113 531-538 NN denotes portion
T5114 539-541 IN denotes of
T5115 542-546 NN denotes Exon
T5116 547-548 CD denotes 4
T5117 549-552 CC denotes and
T5118 553-556 DT denotes all
T5119 557-559 IN denotes of
T5120 560-564 NN denotes Exon
T5121 565-566 CD denotes 5
T5122 567-571 IN denotes with
T5123 572-575 DT denotes the
T5124 590-594 NN denotes gene
T5125 576-582 NN denotes PGKneo
T5126 583-589 NN denotes marker
T5127 595-596 -LRB- denotes (
T5128 596-599 VB denotes See
T5129 600-604 NN denotes Fig.
T5130 605-607 NN denotes 1A
T5131 607-608 -RRB- denotes )
T5132 609-613 IN denotes from
T5133 614-617 DT denotes the
T5134 623-629 NN denotes vector
T5135 618-622 NN denotes pPNT
T5136 630-631 -LRB- denotes [
T5137 631-633 CD denotes 22
T5138 633-634 -RRB- denotes ]
T5139 634-635 . denotes .
T5140 635-769 sentence denotes The targeting construct was linearized with NotI and electroporated into R1 ES cells [23] under conditions described previously [24].
T5141 636-639 DT denotes The
T5142 650-659 NN denotes construct
T5143 640-649 NN denotes targeting
T5144 664-674 VBN denotes linearized
T5145 660-663 VBD denotes was
T5146 675-679 IN denotes with
T5147 680-684 NN denotes NotI
T5148 685-688 CC denotes and
T5149 689-703 VBN denotes electroporated
T5150 704-708 IN denotes into
T5151 709-711 NN denotes R1
T5152 715-720 NNS denotes cells
T5153 712-714 NN denotes ES
T5154 721-722 -LRB- denotes [
T5155 722-724 CD denotes 23
T5156 724-725 -RRB- denotes ]
T5157 726-731 IN denotes under
T5158 732-742 NNS denotes conditions
T5159 743-752 VBN denotes described
T5160 753-763 RB denotes previously
T5161 764-765 -LRB- denotes [
T5162 765-767 CD denotes 24
T5163 767-768 -RRB- denotes ]
T5164 768-769 . denotes .
T5165 769-911 sentence denotes ES cells were selected with G418 (300 μg/ml; Life Technologies Inc., Gaithersburg, MD) and gancyclovir (2 μM; gift of Syntex, Palo Alto, CA).
T5166 770-772 NN denotes ES
T5167 773-778 NNS denotes cells
T5168 784-792 VBN denotes selected
T5169 779-783 VBD denotes were
T5170 793-797 IN denotes with
T5171 798-802 NN denotes G418
T5172 803-804 -LRB- denotes (
T5173 833-837 NNP denotes Inc.
T5174 804-807 CD denotes 300
T5175 808-810 NN denotes μg
T5176 810-811 SYM denotes /
T5177 811-813 NN denotes ml
T5178 813-814 : denotes ;
T5179 815-819 NNP denotes Life
T5180 820-832 NNP denotes Technologies
T5181 837-839 , denotes ,
T5182 839-851 NNP denotes Gaithersburg
T5183 851-853 , denotes ,
T5184 853-855 NNP denotes MD
T5185 855-856 -RRB- denotes )
T5186 857-860 CC denotes and
T5187 861-872 NN denotes gancyclovir
T5188 873-874 -LRB- denotes (
T5189 874-875 CD denotes 2
T5190 876-878 NN denotes μM
T5191 878-879 : denotes ;
T5192 880-884 NN denotes gift
T5193 885-887 IN denotes of
T5194 888-894 NNP denotes Syntex
T5195 894-896 , denotes ,
T5196 896-900 NNP denotes Palo
T5197 901-905 NNP denotes Alto
T5198 905-907 , denotes ,
T5199 907-909 NNP denotes CA
T5200 909-910 -RRB- denotes )
T5201 910-911 . denotes .
T5202 911-1024 sentence denotes Doubly resistant clones were screened for gene targeting by Southern blot analysis of BglI digested genomic DNA.
T5203 912-918 RB denotes Doubly
T5204 919-928 JJ denotes resistant
T5205 929-935 NNS denotes clones
T5206 941-949 VBN denotes screened
T5207 936-940 VBD denotes were
T5208 950-953 IN denotes for
T5209 954-958 NN denotes gene
T5210 959-968 NN denotes targeting
T5211 969-971 IN denotes by
T5212 972-980 NNP denotes Southern
T5213 981-985 NN denotes blot
T5214 986-994 NN denotes analysis
T5215 995-997 IN denotes of
T5216 998-1002 NN denotes BglI
T5217 1020-1023 NN denotes DNA
T5218 1003-1011 VBN denotes digested
T5219 1012-1019 JJ denotes genomic
T5220 1023-1024 . denotes .
T5221 1024-1127 sentence denotes Blots were hybridized with an Exon 6 specific probe that was external to the gene targeting construct.
T5222 1025-1030 NNS denotes Blots
T5223 1036-1046 VBN denotes hybridized
T5224 1031-1035 VBD denotes were
T5225 1047-1051 IN denotes with
T5226 1052-1054 DT denotes an
T5227 1071-1076 NN denotes probe
T5228 1055-1059 NN denotes Exon
T5229 1062-1070 JJ denotes specific
T5230 1060-1061 CD denotes 6
T5231 1077-1081 WDT denotes that
T5232 1082-1085 VBD denotes was
T5233 1086-1094 JJ denotes external
T5234 1095-1097 IN denotes to
T5235 1098-1101 DT denotes the
T5236 1117-1126 NN denotes construct
T5237 1102-1106 NN denotes gene
T5238 1107-1116 NN denotes targeting
T5239 1126-1127 . denotes .
T5240 1127-1247 sentence denotes Correctly targeted ES cells were injected into C57BL/6J blastocysts to produce chimeric mice using standard procedures.
T5241 1128-1137 RB denotes Correctly
T5242 1138-1146 VBN denotes targeted
T5243 1150-1155 NNS denotes cells
T5244 1147-1149 NN denotes ES
T5245 1161-1169 VBN denotes injected
T5246 1156-1160 VBD denotes were
T5247 1170-1174 IN denotes into
T5248 1175-1180 NN denotes C57BL
T5249 1181-1183 NN denotes 6J
T5250 1180-1181 HYPH denotes /
T5251 1184-1195 NNS denotes blastocysts
T5252 1196-1198 TO denotes to
T5253 1199-1206 VB denotes produce
T5254 1207-1215 JJ denotes chimeric
T5255 1216-1220 NNS denotes mice
T5256 1221-1226 VBG denotes using
T5257 1227-1235 JJ denotes standard
T5258 1236-1246 NNS denotes procedures
T5259 1246-1247 . denotes .
T5260 1247-1404 sentence denotes Heterozygous offspring from germline competent chimeras were intercrossed to produce wild type (+/+), heterozygous (+/-) and homozygous knockout (-/-) mice.
T5261 1248-1260 JJ denotes Heterozygous
T5262 1261-1270 NN denotes offspring
T5263 1309-1321 VBN denotes intercrossed
T5264 1271-1275 IN denotes from
T5265 1276-1284 NN denotes germline
T5266 1285-1294 JJ denotes competent
T5267 1295-1303 NNS denotes chimeras
T5268 1304-1308 VBD denotes were
T5269 1322-1324 TO denotes to
T5270 1325-1332 VB denotes produce
T5271 1333-1337 JJ denotes wild
T5272 1338-1342 NN denotes type
T5273 1384-1392 NN denotes knockout
T5274 1343-1344 -LRB- denotes (
T5275 1346-1347 SYM denotes +
T5276 1344-1345 SYM denotes +
T5277 1345-1346 HYPH denotes /
T5278 1347-1348 -RRB- denotes )
T5279 1348-1350 , denotes ,
T5280 1350-1362 JJ denotes heterozygous
T5281 1363-1364 -LRB- denotes (
T5282 1366-1367 SYM denotes -
T5283 1364-1365 SYM denotes +
T5284 1365-1366 HYPH denotes /
T5285 1367-1368 -RRB- denotes )
T5286 1369-1372 CC denotes and
T5287 1373-1383 JJ denotes homozygous
T5288 1399-1403 NNS denotes mice
T5289 1393-1394 -LRB- denotes (
T5290 1396-1397 SYM denotes -
T5291 1394-1395 SYM denotes -
T5292 1395-1396 HYPH denotes /
T5293 1397-1398 -RRB- denotes )
T5294 1403-1404 . denotes .
T5295 1404-1454 sentence denotes At all generations, +/- breeding pairs were used.
T5296 1405-1407 IN denotes At
T5297 1449-1453 VBN denotes used
T5298 1408-1411 DT denotes all
T5299 1412-1423 NNS denotes generations
T5300 1423-1425 , denotes ,
T5301 1425-1426 SYM denotes +
T5302 1427-1428 SYM denotes -
T5303 1426-1427 HYPH denotes /
T5304 1438-1443 NNS denotes pairs
T5305 1429-1437 VBG denotes breeding
T5306 1444-1448 VBD denotes were
T5307 1453-1454 . denotes .
T5308 1454-1525 sentence denotes Results presented here are from mice derived from the F2+ generations.
T5309 1455-1462 NNS denotes Results
T5310 1478-1481 VBP denotes are
T5311 1463-1472 VBN denotes presented
T5312 1473-1477 RB denotes here
T5313 1482-1486 IN denotes from
T5314 1487-1491 NNS denotes mice
T5315 1492-1499 VBN denotes derived
T5316 1500-1504 IN denotes from
T5317 1505-1508 DT denotes the
T5318 1513-1524 NNS denotes generations
T5319 1509-1511 NN denotes F2
T5320 1511-1512 SYM denotes +
T5321 1524-1525 . denotes .
T5322 1525-1601 sentence denotes All animals were of a mixed C57BL/6J × Strain 129Sv/SvJ genetic background.
T5323 1526-1529 DT denotes All
T5324 1530-1537 NNS denotes animals
T5325 1538-1542 VBD denotes were
T5326 1543-1545 IN denotes of
T5327 1546-1547 DT denotes a
T5328 1590-1600 NN denotes background
T5329 1548-1553 VBN denotes mixed
T5330 1554-1559 NN denotes C57BL
T5331 1560-1562 NN denotes 6J
T5332 1559-1560 HYPH denotes /
T5333 1563-1564 SYM denotes ×
T5334 1565-1571 NN denotes Strain
T5335 1578-1581 NN denotes SvJ
T5336 1572-1577 NN denotes 129Sv
T5337 1577-1578 HYPH denotes /
T5338 1582-1589 JJ denotes genetic
T5339 1600-1601 . denotes .
T5872 3083-3093 NN denotes Production
T5873 3094-3096 IN denotes of
T5874 3097-3107 JJ denotes transgenic
T5875 3108-3112 NNS denotes mice
T5876 3112-3199 sentence denotes Standard molecular techniques were used to assemble the transgenic construct, pTRE-E2.
T5877 3113-3121 JJ denotes Standard
T5878 3132-3142 NNS denotes techniques
T5879 3122-3131 JJ denotes molecular
T5880 3148-3152 VBN denotes used
T5881 3143-3147 VBD denotes were
T5882 3153-3155 TO denotes to
T5883 3156-3164 VB denotes assemble
T5884 3165-3168 DT denotes the
T5885 3180-3189 NN denotes construct
T5886 3169-3179 JJ denotes transgenic
T5887 3189-3191 , denotes ,
T5888 3191-3195 NN denotes pTRE
T5889 3196-3198 NN denotes E2
T5890 3195-3196 HYPH denotes -
T5891 3198-3199 . denotes .
T5892 3199-3823 sentence denotes This transgene contains the tetracycline responsive hCMV*-1 promoter [consisting of the tetracycline responsive element (TRE) and a minimal hCMV promoter] [25] from pTRE2 (Clontech Inc., Mt. View, CA), a chimeric intron from pCI (Promega) to increase message stability and expression [26,27], a Kozak consensus sequence at the initiation codon to optimize translation [28], the human E2 cDNA which has been modified to contain a 4× alanine linker followed by a c-myc epitope tag at the carboxy terminus to facilitate detection, and an SV40 late polyadenylation sequence from pCI for enhanced mRNA stability and translation.
T5893 3200-3204 DT denotes This
T5894 3205-3214 NN denotes transgene
T5895 3215-3223 VBZ denotes contains
T5896 3224-3227 DT denotes the
T5897 3260-3268 NN denotes promoter
T5898 3228-3240 NN denotes tetracycline
T5899 3241-3251 JJ denotes responsive
T5900 3252-3256 NN denotes hCMV
T5901 3256-3257 SYM denotes *
T5902 3257-3258 HYPH denotes -
T5903 3258-3259 CD denotes 1
T5904 3269-3270 -LRB- denotes [
T5905 3270-3280 VBG denotes consisting
T5906 3281-3283 IN denotes of
T5907 3284-3287 DT denotes the
T5908 3312-3319 NN denotes element
T5909 3288-3300 NN denotes tetracycline
T5910 3301-3311 JJ denotes responsive
T5911 3320-3321 -LRB- denotes (
T5912 3321-3324 NN denotes TRE
T5913 3324-3325 -RRB- denotes )
T5914 3326-3329 CC denotes and
T5915 3330-3331 DT denotes a
T5916 3345-3353 NN denotes promoter
T5917 3332-3339 JJ denotes minimal
T5918 3340-3344 NN denotes hCMV
T5919 3353-3354 -RRB- denotes ]
T5920 3355-3356 -LRB- denotes [
T5921 3356-3358 CD denotes 25
T5922 3358-3359 -RRB- denotes ]
T5923 3360-3364 IN denotes from
T5924 3365-3370 NN denotes pTRE2
T5925 3371-3372 -LRB- denotes (
T5926 3381-3385 NNP denotes Inc.
T5927 3372-3380 NNP denotes Clontech
T5928 3385-3387 , denotes ,
T5929 3387-3390 NNP denotes Mt.
T5930 3391-3395 NNP denotes View
T5931 3395-3397 , denotes ,
T5932 3397-3399 NNP denotes CA
T5933 3399-3400 -RRB- denotes )
T5934 3400-3402 , denotes ,
T5935 3402-3403 DT denotes a
T5936 3413-3419 NN denotes intron
T5937 3404-3412 JJ denotes chimeric
T5938 3420-3424 IN denotes from
T5939 3425-3428 NN denotes pCI
T5940 3429-3430 -LRB- denotes (
T5941 3430-3437 NNP denotes Promega
T5942 3437-3438 -RRB- denotes )
T5943 3439-3441 TO denotes to
T5944 3442-3450 VB denotes increase
T5945 3451-3458 NN denotes message
T5946 3459-3468 NN denotes stability
T5947 3469-3472 CC denotes and
T5948 3473-3483 NN denotes expression
T5949 3484-3485 -LRB- denotes [
T5950 3488-3490 CD denotes 27
T5951 3485-3487 CD denotes 26
T5952 3487-3488 , denotes ,
T5953 3490-3491 -RRB- denotes ]
T5954 3491-3493 , denotes ,
T5955 3493-3494 DT denotes a
T5956 3511-3519 NN denotes sequence
T5957 3495-3500 NN denotes Kozak
T5958 3501-3510 NN denotes consensus
T5959 3520-3522 IN denotes at
T5960 3523-3526 DT denotes the
T5961 3538-3543 NN denotes codon
T5962 3527-3537 NN denotes initiation
T5963 3544-3546 TO denotes to
T5964 3547-3555 VB denotes optimize
T5965 3556-3567 NN denotes translation
T5966 3568-3569 -LRB- denotes [
T5967 3569-3571 CD denotes 28
T5968 3571-3572 -RRB- denotes ]
T5969 3572-3574 , denotes ,
T5970 3574-3577 DT denotes the
T5971 3587-3591 NN denotes cDNA
T5972 3578-3583 JJ denotes human
T5973 3584-3586 NN denotes E2
T5974 3592-3597 WDT denotes which
T5975 3607-3615 VBN denotes modified
T5976 3598-3601 VBZ denotes has
T5977 3602-3606 VBN denotes been
T5978 3616-3618 TO denotes to
T5979 3619-3626 VB denotes contain
T5980 3627-3628 DT denotes a
T5981 3640-3646 NN denotes linker
T5982 3629-3630 CD denotes 4
T5983 3630-3631 SYM denotes ×
T5984 3632-3639 NN denotes alanine
T5985 3647-3655 VBN denotes followed
T5986 3656-3658 IN denotes by
T5987 3659-3660 DT denotes a
T5988 3675-3678 NN denotes tag
T5989 3661-3662 NN denotes c
T5990 3663-3666 NN denotes myc
T5991 3662-3663 HYPH denotes -
T5992 3667-3674 NN denotes epitope
T5993 3679-3681 IN denotes at
T5994 3682-3685 DT denotes the
T5995 3694-3702 NN denotes terminus
T5996 3686-3693 NN denotes carboxy
T5997 3703-3705 TO denotes to
T5998 3706-3716 VB denotes facilitate
T5999 3717-3726 NN denotes detection
T6000 3726-3728 , denotes ,
T6001 3728-3731 CC denotes and
T6002 3732-3734 DT denotes an
T6003 3761-3769 NN denotes sequence
T6004 3735-3739 NN denotes SV40
T6005 3740-3744 JJ denotes late
T6006 3745-3760 NN denotes polyadenylation
T6007 3770-3774 IN denotes from
T6008 3775-3778 NN denotes pCI
T6009 3779-3782 IN denotes for
T6010 3783-3791 VBN denotes enhanced
T6011 3797-3806 NN denotes stability
T6012 3792-3796 NN denotes mRNA
T6013 3807-3810 CC denotes and
T6014 3811-3822 NN denotes translation
T6015 3822-3823 . denotes .
T6016 3823-4111 sentence denotes The 2.48 kb TRE-E2 transgene was purified from vector sequences following digestion with XhoI and BamHI and injected into C57BL/6J or C57BL/6J × Strain 129SvEv mouse embryos at the transgenic core facilities of the University of Pittsburgh and the University of Cincinnati, respectively.
T6017 3824-3827 DT denotes The
T6018 3843-3852 NN denotes transgene
T6019 3828-3832 CD denotes 2.48
T6020 3833-3835 NN denotes kb
T6021 3836-3839 NN denotes TRE
T6022 3840-3842 NN denotes E2
T6023 3839-3840 HYPH denotes -
T6024 3857-3865 VBN denotes purified
T6025 3853-3856 VBD denotes was
T6026 3866-3870 IN denotes from
T6027 3871-3877 NN denotes vector
T6028 3878-3887 NNS denotes sequences
T6029 3888-3897 VBG denotes following
T6030 3898-3907 NN denotes digestion
T6031 3908-3912 IN denotes with
T6032 3913-3917 NN denotes XhoI
T6033 3918-3921 CC denotes and
T6034 3922-3927 NN denotes BamHI
T6035 3928-3931 CC denotes and
T6036 3932-3940 VBN denotes injected
T6037 3941-3945 IN denotes into
T6038 3946-3951 NN denotes C57BL
T6039 3952-3954 NN denotes 6J
T6040 3951-3952 HYPH denotes /
T6041 3969-3975 NN denotes Strain
T6042 3955-3957 CC denotes or
T6043 3958-3963 NN denotes C57BL
T6044 3964-3966 NN denotes 6J
T6045 3963-3964 HYPH denotes /
T6046 3967-3968 SYM denotes ×
T6047 3990-3997 NNS denotes embryos
T6048 3976-3983 NN denotes 129SvEv
T6049 3984-3989 NN denotes mouse
T6050 3998-4000 IN denotes at
T6051 4001-4004 DT denotes the
T6052 4021-4031 NNS denotes facilities
T6053 4005-4015 JJ denotes transgenic
T6054 4016-4020 NN denotes core
T6055 4032-4034 IN denotes of
T6056 4035-4038 DT denotes the
T6057 4039-4049 NNP denotes University
T6058 4050-4052 IN denotes of
T6059 4053-4063 NNP denotes Pittsburgh
T6060 4064-4067 CC denotes and
T6061 4068-4071 DT denotes the
T6062 4072-4082 NNP denotes University
T6063 4083-4085 IN denotes of
T6064 4086-4096 NNP denotes Cincinnati
T6065 4096-4098 , denotes ,
T6066 4098-4110 RB denotes respectively
T6067 4110-4111 . denotes .
T6068 4111-4308 sentence denotes Genomic DNA from the tail of mice was screened by Southern blot analysis following digestion with EcoRI and hybridization to an ~400 bp probe derived from the SV40 portion of the TRE-E2 transgene.
T6069 4112-4119 JJ denotes Genomic
T6070 4120-4123 NN denotes DNA
T6071 4150-4158 VBN denotes screened
T6072 4124-4128 IN denotes from
T6073 4129-4132 DT denotes the
T6074 4133-4137 NN denotes tail
T6075 4138-4140 IN denotes of
T6076 4141-4145 NNS denotes mice
T6077 4146-4149 VBD denotes was
T6078 4159-4161 IN denotes by
T6079 4162-4170 NNP denotes Southern
T6080 4171-4175 NN denotes blot
T6081 4176-4184 NN denotes analysis
T6082 4185-4194 VBG denotes following
T6083 4195-4204 NN denotes digestion
T6084 4205-4209 IN denotes with
T6085 4210-4215 NN denotes EcoRI
T6086 4216-4219 CC denotes and
T6087 4220-4233 NN denotes hybridization
T6088 4234-4236 IN denotes to
T6089 4237-4239 DT denotes an
T6090 4248-4253 NN denotes probe
T6091 4240-4241 SYM denotes ~
T6092 4241-4244 CD denotes 400
T6093 4245-4247 NN denotes bp
T6094 4254-4261 VBN denotes derived
T6095 4262-4266 IN denotes from
T6096 4267-4270 DT denotes the
T6097 4276-4283 NN denotes portion
T6098 4271-4275 NN denotes SV40
T6099 4284-4286 IN denotes of
T6100 4287-4290 DT denotes the
T6101 4298-4307 NN denotes transgene
T6102 4291-4294 NN denotes TRE
T6103 4295-4297 NN denotes E2
T6104 4294-4295 HYPH denotes -
T6105 4307-4308 . denotes .
T6426 4310-4320 NN denotes Production
T6427 4321-4323 IN denotes of
T6428 4324-4336 JJ denotes intermediate
T6429 4349-4354 NN denotes model
T6430 4337-4341 NN denotes MSUD
T6431 4342-4348 JJ denotes murine
T6432 4354-4611 sentence denotes The various TRE-E2 transgenic lines produced were mated independently to mice that were heterozygous for both the E2 knockout and the LAP-tTA transgene [Tg(tTALap)Bjd/J; Stock 3272; The Jackson Laboratory, Bar Harbor, ME; NMRI × FVB × C57BL/6J background].
T6433 4355-4358 DT denotes The
T6434 4385-4390 NNS denotes lines
T6435 4359-4366 JJ denotes various
T6436 4367-4370 NN denotes TRE
T6437 4371-4373 NN denotes E2
T6438 4370-4371 HYPH denotes -
T6439 4374-4384 JJ denotes transgenic
T6440 4405-4410 VBN denotes mated
T6441 4391-4399 VBN denotes produced
T6442 4400-4404 VBD denotes were
T6443 4411-4424 RB denotes independently
T6444 4425-4427 IN denotes to
T6445 4428-4432 NNS denotes mice
T6446 4433-4437 WDT denotes that
T6447 4438-4442 VBD denotes were
T6448 4443-4455 JJ denotes heterozygous
T6449 4456-4459 IN denotes for
T6450 4460-4464 CC denotes both
T6451 4472-4480 NN denotes knockout
T6452 4465-4468 DT denotes the
T6453 4469-4471 NN denotes E2
T6454 4481-4484 CC denotes and
T6455 4485-4488 DT denotes the
T6456 4497-4506 NN denotes transgene
T6457 4489-4492 NN denotes LAP
T6458 4493-4496 NN denotes tTA
T6459 4492-4493 HYPH denotes -
T6460 4507-4508 -LRB- denotes [
T6461 4599-4609 NN denotes background
T6462 4508-4510 NN denotes Tg
T6463 4525-4530 NN denotes Stock
T6464 4510-4511 -LRB- denotes (
T6465 4511-4517 NN denotes tTALap
T6466 4517-4518 -RRB- denotes )
T6467 4518-4521 NN denotes Bjd
T6468 4522-4523 NN denotes J
T6469 4521-4522 HYPH denotes /
T6470 4523-4524 : denotes ;
T6471 4531-4535 CD denotes 3272
T6472 4535-4536 : denotes ;
T6473 4537-4540 DT denotes The
T6474 4549-4559 NNP denotes Laboratory
T6475 4541-4548 NNP denotes Jackson
T6476 4559-4561 , denotes ,
T6477 4561-4564 NNP denotes Bar
T6478 4565-4571 NNP denotes Harbor
T6479 4571-4573 , denotes ,
T6480 4573-4575 NNP denotes ME
T6481 4575-4576 : denotes ;
T6482 4577-4581 NN denotes NMRI
T6483 4582-4583 SYM denotes ×
T6484 4584-4587 NN denotes FVB
T6485 4588-4589 SYM denotes ×
T6486 4590-4595 NN denotes C57BL
T6487 4596-4598 NN denotes 6J
T6488 4595-4596 HYPH denotes /
T6489 4609-4610 -RRB- denotes ]
T6490 4610-4611 . denotes .
T6491 4611-4912 sentence denotes Interbreeding of animals that were heterozygous for both transgenes and the knockout resulted in the production of mice with a variety of genotypes including some animals that were homozygous for the knockout and were positive for both transgenes (we refer to this genotype as the "rescue" genotype).
T6492 4612-4625 NN denotes Interbreeding
T6493 4697-4705 VBD denotes resulted
T6494 4626-4628 IN denotes of
T6495 4629-4636 NNS denotes animals
T6496 4637-4641 WDT denotes that
T6497 4642-4646 VBD denotes were
T6498 4647-4659 JJ denotes heterozygous
T6499 4660-4663 IN denotes for
T6500 4664-4668 DT denotes both
T6501 4669-4679 NNS denotes transgenes
T6502 4680-4683 CC denotes and
T6503 4684-4687 DT denotes the
T6504 4688-4696 NN denotes knockout
T6505 4706-4708 IN denotes in
T6506 4709-4712 DT denotes the
T6507 4713-4723 NN denotes production
T6508 4724-4726 IN denotes of
T6509 4727-4731 NNS denotes mice
T6510 4732-4736 IN denotes with
T6511 4737-4738 DT denotes a
T6512 4739-4746 NN denotes variety
T6513 4747-4749 IN denotes of
T6514 4750-4759 NNS denotes genotypes
T6515 4760-4769 VBG denotes including
T6516 4770-4774 DT denotes some
T6517 4775-4782 NNS denotes animals
T6518 4783-4787 WDT denotes that
T6519 4788-4792 VBD denotes were
T6520 4793-4803 JJ denotes homozygous
T6521 4804-4807 IN denotes for
T6522 4808-4811 DT denotes the
T6523 4812-4820 NN denotes knockout
T6524 4821-4824 CC denotes and
T6525 4825-4829 VBD denotes were
T6526 4830-4838 JJ denotes positive
T6527 4839-4842 IN denotes for
T6528 4843-4847 DT denotes both
T6529 4848-4858 NNS denotes transgenes
T6530 4859-4860 -LRB- denotes (
T6531 4863-4868 VBP denotes refer
T6532 4860-4862 PRP denotes we
T6533 4869-4871 IN denotes to
T6534 4872-4876 DT denotes this
T6535 4877-4885 NN denotes genotype
T6536 4886-4888 IN denotes as
T6537 4889-4892 DT denotes the
T6538 4902-4910 NN denotes genotype
T6539 4893-4894 `` denotes "
T6540 4894-4900 NN denotes rescue
T6541 4900-4901 '' denotes "
T6542 4910-4911 -RRB- denotes )
T6543 4911-4912 . denotes .
T6544 4912-5110 sentence denotes If our strategy for rescuing the neonatal lethal phenotype of the knockout were successful, then those homozygous knockout animals that had both transgenes would survive beyond the neonatal period.
T6545 4913-4915 IN denotes If
T6546 4988-4992 VBD denotes were
T6547 4916-4919 PRP$ denotes our
T6548 4920-4928 NN denotes strategy
T6549 4929-4932 IN denotes for
T6550 4933-4941 VBG denotes rescuing
T6551 4942-4945 DT denotes the
T6552 4962-4971 NN denotes phenotype
T6553 4946-4954 JJ denotes neonatal
T6554 4955-4961 JJ denotes lethal
T6555 4972-4974 IN denotes of
T6556 4975-4978 DT denotes the
T6557 4979-4987 NN denotes knockout
T6558 5075-5082 VB denotes survive
T6559 4993-5003 JJ denotes successful
T6560 5003-5005 , denotes ,
T6561 5005-5009 RB denotes then
T6562 5010-5015 DT denotes those
T6563 5036-5043 NNS denotes animals
T6564 5016-5026 JJ denotes homozygous
T6565 5027-5035 NN denotes knockout
T6566 5044-5048 WDT denotes that
T6567 5049-5052 VBD denotes had
T6568 5053-5057 DT denotes both
T6569 5058-5068 NNS denotes transgenes
T6570 5069-5074 MD denotes would
T6571 5083-5089 IN denotes beyond
T6572 5090-5093 DT denotes the
T6573 5103-5109 NN denotes period
T6574 5094-5102 JJ denotes neonatal
T6575 5109-5110 . denotes .
T6670 5112-5122 NN denotes Genotyping
T6671 5122-5173 sentence denotes All mice were genotyped by Southern blot analysis.
T6672 5123-5126 DT denotes All
T6673 5127-5131 NNS denotes mice
T6674 5137-5146 VBN denotes genotyped
T6675 5132-5136 VBD denotes were
T6676 5147-5149 IN denotes by
T6677 5150-5158 NNP denotes Southern
T6678 5159-5163 NN denotes blot
T6679 5164-5172 NN denotes analysis
T6680 5172-5173 . denotes .
T6681 5173-5371 sentence denotes Genomic DNAs prepared from tail snips were digested with an appropriate restriction enzyme, size fractionated by agarose gel electrophoresis, blotted to nylon, and probed using standard procedures.
T6682 5174-5181 JJ denotes Genomic
T6683 5182-5186 NNS denotes DNAs
T6684 5217-5225 VBN denotes digested
T6685 5187-5195 VBN denotes prepared
T6686 5196-5200 IN denotes from
T6687 5201-5205 NN denotes tail
T6688 5206-5211 NNS denotes snips
T6689 5212-5216 VBD denotes were
T6690 5226-5230 IN denotes with
T6691 5231-5233 DT denotes an
T6692 5258-5264 NN denotes enzyme
T6693 5234-5245 JJ denotes appropriate
T6694 5246-5257 NN denotes restriction
T6695 5264-5266 , denotes ,
T6696 5266-5270 NN denotes size
T6697 5271-5283 VBN denotes fractionated
T6698 5284-5286 IN denotes by
T6699 5287-5294 NN denotes agarose
T6700 5295-5298 NN denotes gel
T6701 5299-5314 NN denotes electrophoresis
T6702 5314-5316 , denotes ,
T6703 5316-5323 VBN denotes blotted
T6704 5324-5326 IN denotes to
T6705 5327-5332 NN denotes nylon
T6706 5332-5334 , denotes ,
T6707 5334-5337 CC denotes and
T6708 5338-5344 VBN denotes probed
T6709 5345-5350 VBG denotes using
T6710 5351-5359 JJ denotes standard
T6711 5360-5370 NNS denotes procedures
T6712 5370-5371 . denotes .
T7183 5373-5393 NN denotes Immunohistochemistry
T7184 5393-5500 sentence denotes Primary mouse embryonic fibroblasts were prepared from embryonic day ~16.5–18.5 fetuses as described [29].
T7185 5394-5401 JJ denotes Primary
T7186 5418-5429 NNS denotes fibroblasts
T7187 5402-5407 NN denotes mouse
T7188 5408-5417 JJ denotes embryonic
T7189 5435-5443 VBN denotes prepared
T7190 5430-5434 VBD denotes were
T7191 5444-5448 IN denotes from
T7192 5449-5458 JJ denotes embryonic
T7193 5474-5481 NNS denotes fetuses
T7194 5459-5462 NN denotes day
T7195 5464-5468 CD denotes 16.5
T7196 5463-5464 SYM denotes ~
T7197 5468-5469 SYM denotes –
T7198 5469-5473 CD denotes 18.5
T7199 5482-5484 IN denotes as
T7200 5485-5494 VBN denotes described
T7201 5495-5496 -LRB- denotes [
T7202 5496-5498 CD denotes 29
T7203 5498-5499 -RRB- denotes ]
T7204 5499-5500 . denotes .
T7205 5500-5756 sentence denotes Fibroblasts were passed onto glass, fixed in 2% paraformaldehyde in PBS for 10 minutes, permeabilized in 2% paraformaldehyde containing 0.1% Triton X100 for 10 minutes and washed three times in PBS containing 0.5% BSA and 0.15% glycine, pH 7.4 (Buffer A).
T7206 5501-5512 NNS denotes Fibroblasts
T7207 5518-5524 VBN denotes passed
T7208 5513-5517 VBD denotes were
T7209 5525-5529 IN denotes onto
T7210 5530-5535 NN denotes glass
T7211 5535-5537 , denotes ,
T7212 5537-5542 VBN denotes fixed
T7213 5543-5545 IN denotes in
T7214 5546-5547 CD denotes 2
T7215 5547-5548 NN denotes %
T7216 5549-5565 NN denotes paraformaldehyde
T7217 5566-5568 IN denotes in
T7218 5569-5572 NN denotes PBS
T7219 5573-5576 IN denotes for
T7220 5577-5579 CD denotes 10
T7221 5580-5587 NNS denotes minutes
T7222 5587-5589 , denotes ,
T7223 5589-5602 VBN denotes permeabilized
T7224 5603-5605 IN denotes in
T7225 5606-5607 CD denotes 2
T7226 5607-5608 NN denotes %
T7227 5609-5625 NN denotes paraformaldehyde
T7228 5626-5636 VBG denotes containing
T7229 5637-5640 CD denotes 0.1
T7230 5640-5641 NN denotes %
T7231 5649-5653 NN denotes X100
T7232 5642-5648 NN denotes Triton
T7233 5654-5657 IN denotes for
T7234 5658-5660 CD denotes 10
T7235 5661-5668 NNS denotes minutes
T7236 5669-5672 CC denotes and
T7237 5673-5679 VBD denotes washed
T7238 5680-5685 CD denotes three
T7239 5686-5691 NNS denotes times
T7240 5692-5694 IN denotes in
T7241 5695-5698 NN denotes PBS
T7242 5699-5709 VBG denotes containing
T7243 5710-5713 CD denotes 0.5
T7244 5713-5714 NN denotes %
T7245 5715-5718 NN denotes BSA
T7246 5719-5722 CC denotes and
T7247 5723-5727 CD denotes 0.15
T7248 5727-5728 NN denotes %
T7249 5729-5736 NN denotes glycine
T7250 5736-5738 , denotes ,
T7251 5738-5740 NN denotes pH
T7252 5741-5744 CD denotes 7.4
T7253 5745-5746 -LRB- denotes (
T7254 5753-5754 NN denotes A
T7255 5746-5752 NN denotes Buffer
T7256 5754-5755 -RRB- denotes )
T7257 5755-5756 . denotes .
T7258 5756-6063 sentence denotes Following a 30 min incubation with purified goat IgG (50 (μg/ml) at 25°C and three additional washes with Buffer A, cells were incubated for 60 min with E2-specific antiserum [30] at 1 μg/ml followed by three washes in Buffer A and 60 minute incubation in fluorescently labeled second antibody (1–2 μg/ml).
T7259 5757-5766 VBG denotes Following
T7260 5884-5893 VBN denotes incubated
T7261 5767-5768 DT denotes a
T7262 5776-5786 NN denotes incubation
T7263 5769-5771 CD denotes 30
T7264 5772-5775 NN denotes min
T7265 5787-5791 IN denotes with
T7266 5792-5800 VBN denotes purified
T7267 5806-5809 NN denotes IgG
T7268 5801-5805 NN denotes goat
T7269 5810-5811 -LRB- denotes (
T7270 5815-5817 NN denotes μg
T7271 5811-5813 CD denotes 50
T7272 5814-5815 -LRB- denotes (
T7273 5817-5818 SYM denotes /
T7274 5818-5820 NN denotes ml
T7275 5820-5821 -RRB- denotes )
T7276 5822-5824 IN denotes at
T7277 5825-5827 CD denotes 25
T7278 5827-5829 NN denotes °C
T7279 5830-5833 CC denotes and
T7280 5834-5839 CD denotes three
T7281 5851-5857 NNS denotes washes
T7282 5840-5850 JJ denotes additional
T7283 5858-5862 IN denotes with
T7284 5863-5869 NN denotes Buffer
T7285 5870-5871 NN denotes A
T7286 5871-5873 , denotes ,
T7287 5873-5878 NNS denotes cells
T7288 5879-5883 VBD denotes were
T7289 5894-5897 IN denotes for
T7290 5898-5900 CD denotes 60
T7291 5901-5904 NN denotes min
T7292 5905-5909 IN denotes with
T7293 5910-5912 NN denotes E2
T7294 5913-5921 JJ denotes specific
T7295 5912-5913 HYPH denotes -
T7296 5922-5931 NN denotes antiserum
T7297 5932-5933 -LRB- denotes [
T7298 5933-5935 CD denotes 30
T7299 5935-5936 -RRB- denotes ]
T7300 5937-5939 IN denotes at
T7301 5940-5941 CD denotes 1
T7302 5942-5944 NN denotes μg
T7303 5944-5945 SYM denotes /
T7304 5945-5947 NN denotes ml
T7305 5948-5956 VBN denotes followed
T7306 5957-5959 IN denotes by
T7307 5960-5965 CD denotes three
T7308 5966-5972 NNS denotes washes
T7309 5973-5975 IN denotes in
T7310 5976-5982 NN denotes Buffer
T7311 5983-5984 NN denotes A
T7312 5985-5988 CC denotes and
T7313 5989-5991 CD denotes 60
T7314 5992-5998 NN denotes minute
T7315 5999-6009 NN denotes incubation
T7316 6010-6012 IN denotes in
T7317 6013-6026 RB denotes fluorescently
T7318 6027-6034 VBN denotes labeled
T7319 6042-6050 NN denotes antibody
T7320 6035-6041 JJ denotes second
T7321 6051-6052 -LRB- denotes (
T7322 6056-6058 NN denotes μg
T7323 6052-6053 CD denotes 1
T7324 6054-6055 CD denotes 2
T7325 6053-6054 SYM denotes –
T7326 6058-6059 SYM denotes /
T7327 6059-6061 NN denotes ml
T7328 6061-6062 -RRB- denotes )
T7329 6062-6063 . denotes .
T7330 6063-6176 sentence denotes The cells were then washed six times (5 min/wash) in Buffer A and then mounted in gelvatol (Monsanto, St Louis).
T7331 6064-6067 DT denotes The
T7332 6068-6073 NNS denotes cells
T7333 6084-6090 VBN denotes washed
T7334 6074-6078 VBD denotes were
T7335 6079-6083 RB denotes then
T7336 6091-6094 CD denotes six
T7337 6095-6100 NNS denotes times
T7338 6101-6102 -LRB- denotes (
T7339 6104-6107 NN denotes min
T7340 6102-6103 CD denotes 5
T7341 6107-6108 SYM denotes /
T7342 6108-6112 NN denotes wash
T7343 6112-6113 -RRB- denotes )
T7344 6114-6116 IN denotes in
T7345 6117-6123 NN denotes Buffer
T7346 6124-6125 NN denotes A
T7347 6126-6129 CC denotes and
T7348 6130-6134 RB denotes then
T7349 6135-6142 VBN denotes mounted
T7350 6143-6145 IN denotes in
T7351 6146-6154 NN denotes gelvatol
T7352 6155-6156 -LRB- denotes (
T7353 6156-6164 NNP denotes Monsanto
T7354 6164-6166 , denotes ,
T7355 6166-6168 NNP denotes St
T7356 6169-6174 NNP denotes Louis
T7357 6174-6175 -RRB- denotes )
T7358 6175-6176 . denotes .
T7359 6176-6381 sentence denotes When livers from newborn pups were examined, fixation was by immersion in 2% paraformaldehyde followed by cryoprotection and shock freezing in liquid nitrogen cooled isopentane and sectioning (5 microns).
T7360 6177-6181 WRB denotes When
T7361 6212-6220 VBN denotes examined
T7362 6182-6188 NNS denotes livers
T7363 6189-6193 IN denotes from
T7364 6194-6201 JJ denotes newborn
T7365 6202-6206 NNS denotes pups
T7366 6207-6211 VBD denotes were
T7367 6231-6234 VBD denotes was
T7368 6220-6222 , denotes ,
T7369 6222-6230 NN denotes fixation
T7370 6235-6237 IN denotes by
T7371 6238-6247 NN denotes immersion
T7372 6248-6250 IN denotes in
T7373 6251-6252 CD denotes 2
T7374 6252-6253 NN denotes %
T7375 6254-6270 NN denotes paraformaldehyde
T7376 6271-6279 VBN denotes followed
T7377 6280-6282 IN denotes by
T7378 6283-6297 NN denotes cryoprotection
T7379 6298-6301 CC denotes and
T7380 6302-6307 NN denotes shock
T7381 6308-6316 NN denotes freezing
T7382 6317-6319 IN denotes in
T7383 6320-6326 JJ denotes liquid
T7384 6327-6335 NN denotes nitrogen
T7385 6336-6342 VBN denotes cooled
T7386 6343-6353 NN denotes isopentane
T7387 6354-6357 CC denotes and
T7388 6358-6368 NN denotes sectioning
T7389 6369-6370 -LRB- denotes (
T7390 6372-6379 NNS denotes microns
T7391 6370-6371 CD denotes 5
T7392 6379-6380 -RRB- denotes )
T7393 6380-6381 . denotes .
T7394 6381-6479 sentence denotes Otherwise processing was as for the cells above (without the fixation and permabilization steps).
T7395 6382-6391 RB denotes Otherwise
T7396 6403-6406 VBD denotes was
T7397 6392-6402 NN denotes processing
T7398 6407-6409 IN denotes as
T7399 6410-6413 IN denotes for
T7400 6414-6417 DT denotes the
T7401 6418-6423 NNS denotes cells
T7402 6424-6429 RB denotes above
T7403 6430-6431 -LRB- denotes (
T7404 6431-6438 IN denotes without
T7405 6439-6442 DT denotes the
T7406 6472-6477 NNS denotes steps
T7407 6443-6451 NN denotes fixation
T7408 6452-6455 CC denotes and
T7409 6456-6471 NN denotes permabilization
T7410 6477-6478 -RRB- denotes )
T7411 6478-6479 . denotes .
T7545 6481-6486 NN denotes Amino
T7546 6487-6491 NN denotes acid
T7547 6492-6500 NN denotes analysis
T7548 6500-6668 sentence denotes Blood was collected from the retroorbital sinus or tail vein of mice and spotted on a filter paper routinely used for blood amino acid analysis for prenatal screening.
T7549 6501-6506 NN denotes Blood
T7550 6511-6520 VBN denotes collected
T7551 6507-6510 VBD denotes was
T7552 6521-6525 IN denotes from
T7553 6526-6529 DT denotes the
T7554 6557-6561 NN denotes vein
T7555 6530-6542 JJ denotes retroorbital
T7556 6543-6548 NN denotes sinus
T7557 6549-6551 CC denotes or
T7558 6552-6556 NN denotes tail
T7559 6562-6564 IN denotes of
T7560 6565-6569 NNS denotes mice
T7561 6570-6573 CC denotes and
T7562 6574-6581 VBN denotes spotted
T7563 6582-6584 IN denotes on
T7564 6585-6586 DT denotes a
T7565 6594-6599 NN denotes paper
T7566 6587-6593 NN denotes filter
T7567 6600-6609 RB denotes routinely
T7568 6610-6614 VBN denotes used
T7569 6615-6618 IN denotes for
T7570 6619-6624 NN denotes blood
T7571 6636-6644 NN denotes analysis
T7572 6625-6630 NN denotes amino
T7573 6631-6635 NN denotes acid
T7574 6645-6648 IN denotes for
T7575 6649-6657 JJ denotes prenatal
T7576 6658-6667 NN denotes screening
T7577 6667-6668 . denotes .
T7578 6668-6806 sentence denotes Concentrations of BCAA and other amino acids in blood were determined by tandem mass spectrometry (Pediatrix Screening, Bridgeville, PA).
T7579 6669-6683 NNS denotes Concentrations
T7580 6728-6738 VBN denotes determined
T7581 6684-6686 IN denotes of
T7582 6687-6691 NN denotes BCAA
T7583 6692-6695 CC denotes and
T7584 6696-6701 JJ denotes other
T7585 6708-6713 NNS denotes acids
T7586 6702-6707 NN denotes amino
T7587 6714-6716 IN denotes in
T7588 6717-6722 NN denotes blood
T7589 6723-6727 VBD denotes were
T7590 6739-6741 IN denotes by
T7591 6742-6748 JJ denotes tandem
T7592 6754-6766 NN denotes spectrometry
T7593 6749-6753 NN denotes mass
T7594 6767-6768 -LRB- denotes (
T7595 6778-6787 NNP denotes Screening
T7596 6768-6777 NNP denotes Pediatrix
T7597 6787-6789 , denotes ,
T7598 6789-6800 NNP denotes Bridgeville
T7599 6800-6802 , denotes ,
T7600 6802-6804 NNP denotes PA
T7601 6804-6805 -RRB- denotes )
T7602 6805-6806 . denotes .
T8105 6808-6813 NN denotes Assay
T8106 6814-6816 IN denotes of
T8107 6817-6822 NN denotes BCKDH
T8108 6823-6831 NN denotes activity
T8109 6831-6900 sentence denotes Livers were removed, frozen in liquid nitrogen, and stored at -80°C.
T8110 6832-6838 NNS denotes Livers
T8111 6844-6851 VBN denotes removed
T8112 6839-6843 VBD denotes were
T8113 6851-6853 , denotes ,
T8114 6853-6859 VBN denotes frozen
T8115 6860-6862 IN denotes in
T8116 6863-6869 JJ denotes liquid
T8117 6870-6878 NN denotes nitrogen
T8118 6878-6880 , denotes ,
T8119 6880-6883 CC denotes and
T8120 6884-6890 VBN denotes stored
T8121 6891-6893 IN denotes at
T8122 6894-6895 HYPH denotes -
T8123 6895-6897 CD denotes 80
T8124 6897-6899 NN denotes °C
T8125 6899-6900 . denotes .
T8126 6900-7019 sentence denotes At the time of enzyme assay, livers were thawed, and homogenized (1:9, w/v) in 0.25 M sucrose, 10 mM Tris-HC1, pH 7.4.
T8127 6901-6903 IN denotes At
T8128 6942-6948 VBN denotes thawed
T8129 6904-6907 DT denotes the
T8130 6908-6912 NN denotes time
T8131 6913-6915 IN denotes of
T8132 6916-6922 NN denotes enzyme
T8133 6923-6928 NN denotes assay
T8134 6928-6930 , denotes ,
T8135 6930-6936 NNS denotes livers
T8136 6937-6941 VBD denotes were
T8137 6948-6950 , denotes ,
T8138 6950-6953 CC denotes and
T8139 6954-6965 VBN denotes homogenized
T8140 6966-6967 -LRB- denotes (
T8141 6974-6975 NN denotes v
T8142 6967-6968 CD denotes 1
T8143 6969-6970 CD denotes 9
T8144 6968-6969 SYM denotes :
T8145 6970-6972 , denotes ,
T8146 6972-6973 NN denotes w
T8147 6973-6974 HYPH denotes /
T8148 6975-6976 -RRB- denotes )
T8149 6977-6979 IN denotes in
T8150 6980-6984 CD denotes 0.25
T8151 6985-6986 NN denotes M
T8152 6987-6994 NN denotes sucrose
T8153 6994-6996 , denotes ,
T8154 6996-6998 CD denotes 10
T8155 6999-7001 NN denotes mM
T8156 7007-7010 NN denotes HC1
T8157 7002-7006 NN denotes Tris
T8158 7006-7007 HYPH denotes -
T8159 7010-7012 , denotes ,
T8160 7012-7014 NN denotes pH
T8161 7015-7018 CD denotes 7.4
T8162 7018-7019 . denotes .
T8163 7019-7155 sentence denotes Liver homogenates were centrifuged at 600 × g for 10 min at 4°C and the supernatant fraction was saved to determine the BCKDH activity.
T8164 7020-7025 NN denotes Liver
T8165 7026-7037 NNS denotes homogenates
T8166 7043-7054 VBN denotes centrifuged
T8167 7038-7042 VBD denotes were
T8168 7055-7057 IN denotes at
T8169 7058-7061 CD denotes 600
T8170 7064-7065 NN denotes g
T8171 7062-7063 SYM denotes ×
T8172 7066-7069 IN denotes for
T8173 7070-7072 CD denotes 10
T8174 7073-7076 NN denotes min
T8175 7077-7079 IN denotes at
T8176 7080-7081 CD denotes 4
T8177 7081-7083 NN denotes °C
T8178 7084-7087 CC denotes and
T8179 7088-7091 DT denotes the
T8180 7104-7112 NN denotes fraction
T8181 7092-7103 NN denotes supernatant
T8182 7117-7122 VBN denotes saved
T8183 7113-7116 VBD denotes was
T8184 7123-7125 TO denotes to
T8185 7126-7135 VB denotes determine
T8186 7136-7139 DT denotes the
T8187 7146-7154 NN denotes activity
T8188 7140-7145 NN denotes BCKDH
T8189 7154-7155 . denotes .
T8190 7155-7279 sentence denotes The use of tissue homogenates was necessitated by the limited availability of liver tissue, particularly from newborn pups.
T8191 7156-7159 DT denotes The
T8192 7160-7163 NN denotes use
T8193 7190-7202 VBN denotes necessitated
T8194 7164-7166 IN denotes of
T8195 7167-7173 NN denotes tissue
T8196 7174-7185 NNS denotes homogenates
T8197 7186-7189 VBD denotes was
T8198 7203-7205 IN denotes by
T8199 7206-7209 DT denotes the
T8200 7218-7230 NN denotes availability
T8201 7210-7217 JJ denotes limited
T8202 7231-7233 IN denotes of
T8203 7234-7239 NN denotes liver
T8204 7240-7246 NN denotes tissue
T8205 7246-7248 , denotes ,
T8206 7248-7260 RB denotes particularly
T8207 7261-7265 IN denotes from
T8208 7266-7273 JJ denotes newborn
T8209 7274-7278 NNS denotes pups
T8210 7278-7279 . denotes .
T8211 7279-7405 sentence denotes BCKDH activity was determined by measuring the release of 14CO2 from α-keto [1-14C] isocaproate as described previously [31].
T8212 7280-7285 NN denotes BCKDH
T8213 7286-7294 NN denotes activity
T8214 7299-7309 VBN denotes determined
T8215 7295-7298 VBD denotes was
T8216 7310-7312 IN denotes by
T8217 7313-7322 VBG denotes measuring
T8218 7323-7326 DT denotes the
T8219 7327-7334 NN denotes release
T8220 7335-7337 IN denotes of
T8221 7338-7343 NN denotes 14CO2
T8222 7344-7348 IN denotes from
T8223 7349-7350 NN denotes α
T8224 7351-7355 NN denotes keto
T8225 7350-7351 HYPH denotes -
T8226 7364-7375 NN denotes isocaproate
T8227 7356-7357 -LRB- denotes [
T8228 7357-7358 CD denotes 1
T8229 7358-7359 SYM denotes -
T8230 7359-7362 NN denotes 14C
T8231 7362-7363 -RRB- denotes ]
T8232 7376-7378 IN denotes as
T8233 7379-7388 VBN denotes described
T8234 7389-7399 RB denotes previously
T8235 7400-7401 -LRB- denotes [
T8236 7401-7403 CD denotes 31
T8237 7403-7404 -RRB- denotes ]
T8238 7404-7405 . denotes .
T8239 7405-7730 sentence denotes The complete reaction mixture contained (final volume 1 ml) 30 mM potassium phosphate buffer, pH 7.4, 0.20 mM α-ketoisocaproate, 0.40 mM CoASH, 0.40 mM thiamin pyrophosphate, 2 mM NAD+, 2 mM dithiothreitol, 5 mM Mg2+, approximately 250,000 DPM of α-keto [1-14C] isocaproate, and 0.10 ml of liver homogenate (2–3 mg protein).
T8240 7406-7409 DT denotes The
T8241 7428-7435 NN denotes mixture
T8242 7410-7418 JJ denotes complete
T8243 7419-7427 NN denotes reaction
T8244 7436-7445 VBD denotes contained
T8245 7446-7447 -LRB- denotes (
T8246 7453-7459 NN denotes volume
T8247 7447-7452 JJ denotes final
T8248 7460-7461 CD denotes 1
T8249 7462-7464 NN denotes ml
T8250 7464-7465 -RRB- denotes )
T8251 7466-7468 CD denotes 30
T8252 7469-7471 NN denotes mM
T8253 7492-7498 NN denotes buffer
T8254 7472-7481 NN denotes potassium
T8255 7482-7491 NN denotes phosphate
T8256 7498-7500 , denotes ,
T8257 7500-7502 NN denotes pH
T8258 7503-7506 CD denotes 7.4
T8259 7506-7508 , denotes ,
T8260 7508-7512 CD denotes 0.20
T8261 7513-7515 NN denotes mM
T8262 7518-7533 NN denotes ketoisocaproate
T8263 7516-7517 NN denotes α
T8264 7517-7518 HYPH denotes -
T8265 7533-7535 , denotes ,
T8266 7535-7539 CD denotes 0.40
T8267 7540-7542 NN denotes mM
T8268 7543-7548 NN denotes CoASH
T8269 7548-7550 , denotes ,
T8270 7550-7554 CD denotes 0.40
T8271 7555-7557 NN denotes mM
T8272 7566-7579 NN denotes pyrophosphate
T8273 7558-7565 NN denotes thiamin
T8274 7579-7581 , denotes ,
T8275 7581-7582 CD denotes 2
T8276 7583-7585 NN denotes mM
T8277 7586-7589 NN denotes NAD
T8278 7589-7590 SYM denotes +
T8279 7590-7592 , denotes ,
T8280 7592-7593 CD denotes 2
T8281 7594-7596 NN denotes mM
T8282 7597-7611 NN denotes dithiothreitol
T8283 7611-7613 , denotes ,
T8284 7613-7614 CD denotes 5
T8285 7615-7617 NN denotes mM
T8286 7618-7621 NN denotes Mg2
T8287 7621-7622 SYM denotes +
T8288 7622-7624 , denotes ,
T8289 7624-7637 RB denotes approximately
T8290 7638-7645 CD denotes 250,000
T8291 7646-7649 NN denotes DPM
T8292 7650-7652 IN denotes of
T8293 7653-7654 NN denotes α
T8294 7655-7659 NN denotes keto
T8295 7654-7655 HYPH denotes -
T8296 7668-7679 NN denotes isocaproate
T8297 7660-7661 -LRB- denotes [
T8298 7661-7662 CD denotes 1
T8299 7662-7663 SYM denotes -
T8300 7663-7666 NN denotes 14C
T8301 7666-7667 -RRB- denotes ]
T8302 7679-7681 , denotes ,
T8303 7681-7684 CC denotes and
T8304 7685-7689 CD denotes 0.10
T8305 7690-7692 NN denotes ml
T8306 7693-7695 IN denotes of
T8307 7696-7701 NN denotes liver
T8308 7702-7712 NN denotes homogenate
T8309 7713-7714 -LRB- denotes (
T8310 7721-7728 NN denotes protein
T8311 7714-7715 CD denotes 2
T8312 7716-7717 CD denotes 3
T8313 7715-7716 SYM denotes –
T8314 7718-7720 NN denotes mg
T8315 7728-7729 -RRB- denotes )
T8316 7729-7730 . denotes .
T8317 7730-7888 sentence denotes Assays were carried out for 15 min at 37°C, 14CO2 was trapped in hydroxide of Hyamine, and radioactivity was determined by liquid scintillation spectrometry.
T8318 7731-7737 NNS denotes Assays
T8319 7743-7750 VBN denotes carried
T8320 7738-7742 VBD denotes were
T8321 7751-7754 RP denotes out
T8322 7755-7758 IN denotes for
T8323 7759-7761 CD denotes 15
T8324 7762-7765 NN denotes min
T8325 7766-7768 IN denotes at
T8326 7769-7771 CD denotes 37
T8327 7771-7773 NN denotes °C
T8328 7773-7775 , denotes ,
T8329 7775-7780 NN denotes 14CO2
T8330 7785-7792 VBN denotes trapped
T8331 7781-7784 VBD denotes was
T8332 7793-7795 IN denotes in
T8333 7796-7805 NN denotes hydroxide
T8334 7806-7808 IN denotes of
T8335 7809-7816 NN denotes Hyamine
T8336 7816-7818 , denotes ,
T8337 7818-7821 CC denotes and
T8338 7822-7835 NN denotes radioactivity
T8339 7840-7850 VBN denotes determined
T8340 7836-7839 VBD denotes was
T8341 7851-7853 IN denotes by
T8342 7854-7860 JJ denotes liquid
T8343 7861-7874 NN denotes scintillation
T8344 7875-7887 NN denotes spectrometry
T8345 7887-7888 . denotes .
T8817 7890-7897 NNP denotes Western
T8818 7898-7902 NN denotes blot
T8819 7903-7911 NN denotes analysis
T8820 7911-8054 sentence denotes Protein extracts were isolated from homogenized liver (freshly harvested and flash frozen) of wildtype, Line 525A, and Line A transgenic mice.
T8821 7912-7919 NN denotes Protein
T8822 7920-7928 NNS denotes extracts
T8823 7934-7942 VBN denotes isolated
T8824 7929-7933 VBD denotes were
T8825 7943-7947 IN denotes from
T8826 7948-7959 VBN denotes homogenized
T8827 7960-7965 NN denotes liver
T8828 7966-7967 -LRB- denotes (
T8829 7967-7974 RB denotes freshly
T8830 7975-7984 VBN denotes harvested
T8831 7985-7988 CC denotes and
T8832 7989-7994 NN denotes flash
T8833 7995-8001 VBN denotes frozen
T8834 8001-8002 -RRB- denotes )
T8835 8003-8005 IN denotes of
T8836 8006-8014 NN denotes wildtype
T8837 8038-8048 JJ denotes transgenic
T8838 8014-8016 , denotes ,
T8839 8016-8020 NN denotes Line
T8840 8021-8025 NN denotes 525A
T8841 8025-8027 , denotes ,
T8842 8027-8030 CC denotes and
T8843 8031-8035 NN denotes Line
T8844 8036-8037 NN denotes A
T8845 8049-8053 NNS denotes mice
T8846 8053-8054 . denotes .
T8847 8054-8241 sentence denotes Protein (25 μg per sample) was analyzed by electrophoresis on a 10% SDS-PAGE Ready Gel (Bio-Rad, Hercules, CA) and transferred to PVDF membrane (Sequi-Blot; Bio-Rad) via electroblotting.
T8848 8055-8062 NN denotes Protein
T8849 8086-8094 VBN denotes analyzed
T8850 8063-8064 -LRB- denotes (
T8851 8067-8069 NN denotes μg
T8852 8064-8066 CD denotes 25
T8853 8070-8073 IN denotes per
T8854 8074-8080 NN denotes sample
T8855 8080-8081 -RRB- denotes )
T8856 8082-8085 VBD denotes was
T8857 8095-8097 IN denotes by
T8858 8098-8113 NN denotes electrophoresis
T8859 8114-8116 IN denotes on
T8860 8117-8118 DT denotes a
T8861 8138-8141 NN denotes Gel
T8862 8119-8121 CD denotes 10
T8863 8121-8122 NN denotes %
T8864 8123-8126 NN denotes SDS
T8865 8127-8131 NN denotes PAGE
T8866 8126-8127 HYPH denotes -
T8867 8132-8137 JJ denotes Ready
T8868 8142-8143 -LRB- denotes (
T8869 8147-8150 NNP denotes Rad
T8870 8143-8146 NNP denotes Bio
T8871 8146-8147 HYPH denotes -
T8872 8150-8152 , denotes ,
T8873 8152-8160 NNP denotes Hercules
T8874 8160-8162 , denotes ,
T8875 8162-8164 NNP denotes CA
T8876 8164-8165 -RRB- denotes )
T8877 8166-8169 CC denotes and
T8878 8170-8181 VBN denotes transferred
T8879 8182-8184 IN denotes to
T8880 8185-8189 NN denotes PVDF
T8881 8190-8198 NN denotes membrane
T8882 8199-8200 -LRB- denotes (
T8883 8216-8219 NNP denotes Rad
T8884 8200-8205 NNP denotes Sequi
T8885 8206-8210 NNP denotes Blot
T8886 8205-8206 HYPH denotes -
T8887 8210-8211 : denotes ;
T8888 8212-8215 NNP denotes Bio
T8889 8215-8216 HYPH denotes -
T8890 8219-8220 -RRB- denotes )
T8891 8221-8224 IN denotes via
T8892 8225-8240 NN denotes electroblotting
T8893 8240-8241 . denotes .
T8894 8241-8450 sentence denotes All blots were probed for E2 protein using polyclonal rabbit E2 antisera (1:5,000), which detects both mouse (~47 kD) and human (~54 kD) E2 subunits ([30]; gift from Dr. Susan Hutson, Wake Forest University).
T8895 8242-8245 DT denotes All
T8896 8246-8251 NNS denotes blots
T8897 8257-8263 VBN denotes probed
T8898 8252-8256 VBD denotes were
T8899 8264-8267 IN denotes for
T8900 8268-8270 NN denotes E2
T8901 8271-8278 NN denotes protein
T8902 8279-8284 VBG denotes using
T8903 8285-8295 JJ denotes polyclonal
T8904 8306-8314 NNS denotes antisera
T8905 8296-8302 NN denotes rabbit
T8906 8303-8305 NN denotes E2
T8907 8315-8316 -LRB- denotes (
T8908 8318-8323 CD denotes 5,000
T8909 8316-8317 CD denotes 1
T8910 8317-8318 SYM denotes :
T8911 8323-8324 -RRB- denotes )
T8912 8324-8326 , denotes ,
T8913 8326-8331 WDT denotes which
T8914 8332-8339 VBZ denotes detects
T8915 8340-8344 CC denotes both
T8916 8382-8390 NNS denotes subunits
T8917 8345-8350 NN denotes mouse
T8918 8351-8352 -LRB- denotes (
T8919 8356-8358 NN denotes kD
T8920 8352-8353 SYM denotes ~
T8921 8353-8355 CD denotes 47
T8922 8358-8359 -RRB- denotes )
T8923 8360-8363 CC denotes and
T8924 8364-8369 JJ denotes human
T8925 8370-8371 -LRB- denotes (
T8926 8375-8377 NN denotes kD
T8927 8371-8372 SYM denotes ~
T8928 8372-8374 CD denotes 54
T8929 8377-8378 -RRB- denotes )
T8930 8379-8381 NN denotes E2
T8931 8391-8392 -LRB- denotes (
T8932 8398-8402 NN denotes gift
T8933 8392-8393 -LRB- denotes [
T8934 8393-8395 CD denotes 30
T8935 8395-8396 -RRB- denotes ]
T8936 8396-8397 : denotes ;
T8937 8403-8407 IN denotes from
T8938 8408-8411 NNP denotes Dr.
T8939 8418-8424 NNP denotes Hutson
T8940 8412-8417 NNP denotes Susan
T8941 8424-8426 , denotes ,
T8942 8426-8430 NNP denotes Wake
T8943 8431-8437 NNP denotes Forest
T8944 8438-8448 NNP denotes University
T8945 8448-8449 -RRB- denotes )
T8946 8449-8450 . denotes .
T8947 8450-8561 sentence denotes Blots were re-probed with a rabbit anti-c-myc tag antibody (1:10,000; abCam, Cambridge, MA; cat.# ab9106-100).
T8948 8451-8456 NNS denotes Blots
T8949 8462-8471 VBN denotes re-probed
T8950 8457-8461 VBD denotes were
T8951 8472-8476 IN denotes with
T8952 8477-8478 DT denotes a
T8953 8501-8509 NN denotes antibody
T8954 8479-8485 NN denotes rabbit
T8955 8486-8492 JJ denotes anti-c
T8956 8493-8496 NN denotes myc
T8957 8492-8493 HYPH denotes -
T8958 8497-8500 NN denotes tag
T8959 8510-8511 -LRB- denotes (
T8960 8549-8555 NN denotes ab9106
T8961 8511-8512 CD denotes 1
T8962 8513-8519 CD denotes 10,000
T8963 8512-8513 SYM denotes :
T8964 8519-8520 : denotes ;
T8965 8521-8526 NNP denotes abCam
T8966 8526-8528 , denotes ,
T8967 8528-8537 NNP denotes Cambridge
T8968 8537-8539 , denotes ,
T8969 8539-8541 NNP denotes MA
T8970 8541-8542 : denotes ;
T8971 8543-8547 NN denotes cat.
T8972 8547-8548 SYM denotes #
T8973 8555-8556 HYPH denotes -
T8974 8556-8559 CD denotes 100
T8975 8559-8560 -RRB- denotes )
T8976 8560-8561 . denotes .
T8977 8561-8692 sentence denotes Blots were also re-probed with an antibody for β-actin (43 kD; 1:10,000; abCam; cat.# ab8227-50) to allow for loading comparisons.
T8978 8562-8567 NNS denotes Blots
T8979 8578-8587 VBN denotes re-probed
T8980 8568-8572 VBD denotes were
T8981 8573-8577 RB denotes also
T8982 8588-8592 IN denotes with
T8983 8593-8595 DT denotes an
T8984 8596-8604 NN denotes antibody
T8985 8605-8608 IN denotes for
T8986 8609-8610 NN denotes β
T8987 8611-8616 NN denotes actin
T8988 8610-8611 HYPH denotes -
T8989 8617-8618 -LRB- denotes (
T8990 8648-8654 NN denotes ab8227
T8991 8618-8620 CD denotes 43
T8992 8621-8623 NN denotes kD
T8993 8623-8624 : denotes ;
T8994 8625-8626 CD denotes 1
T8995 8627-8633 CD denotes 10,000
T8996 8626-8627 SYM denotes :
T8997 8633-8634 : denotes ;
T8998 8635-8640 NN denotes abCam
T8999 8640-8641 : denotes ;
T9000 8642-8646 NN denotes cat.
T9001 8646-8647 SYM denotes #
T9002 8654-8655 HYPH denotes -
T9003 8655-8657 CD denotes 50
T9004 8657-8658 -RRB- denotes )
T9005 8659-8661 TO denotes to
T9006 8662-8667 VB denotes allow
T9007 8668-8671 IN denotes for
T9008 8672-8679 NN denotes loading
T9009 8680-8691 NNS denotes comparisons
T9010 8691-8692 . denotes .
T9011 8692-8946 sentence denotes A goat anti-rabbit secondary antibody conjugated to horseradish peroxidase (1:10,000; Novus, Littleton, CO; cat.# NB730-H) was used for detection using the Western Lightning chemiluminescence reagent (Perkin Elmer, Boston, MA) and exposed to X-ray film.
T9012 8693-8694 DT denotes A
T9013 8722-8730 NN denotes antibody
T9014 8695-8699 NN denotes goat
T9015 8700-8711 JJ denotes anti-rabbit
T9016 8712-8721 JJ denotes secondary
T9017 8820-8824 VBN denotes used
T9018 8731-8741 VBN denotes conjugated
T9019 8742-8744 IN denotes to
T9020 8745-8756 NN denotes horseradish
T9021 8757-8767 NN denotes peroxidase
T9022 8768-8769 -LRB- denotes (
T9023 8813-8814 NN denotes H
T9024 8769-8770 CD denotes 1
T9025 8771-8777 CD denotes 10,000
T9026 8770-8771 SYM denotes :
T9027 8777-8778 : denotes ;
T9028 8779-8784 NNP denotes Novus
T9029 8784-8786 , denotes ,
T9030 8786-8795 NNP denotes Littleton
T9031 8795-8797 , denotes ,
T9032 8797-8799 NNP denotes CO
T9033 8799-8800 : denotes ;
T9034 8801-8805 NN denotes cat.
T9035 8805-8806 SYM denotes #
T9036 8807-8812 NN denotes NB730
T9037 8812-8813 HYPH denotes -
T9038 8814-8815 -RRB- denotes )
T9039 8816-8819 VBD denotes was
T9040 8825-8828 IN denotes for
T9041 8829-8838 NN denotes detection
T9042 8839-8844 VBG denotes using
T9043 8845-8848 DT denotes the
T9044 8885-8892 NN denotes reagent
T9045 8849-8856 NNP denotes Western
T9046 8857-8866 NNP denotes Lightning
T9047 8867-8884 NN denotes chemiluminescence
T9048 8893-8894 -LRB- denotes (
T9049 8901-8906 NNP denotes Elmer
T9050 8894-8900 NNP denotes Perkin
T9051 8906-8908 , denotes ,
T9052 8908-8914 NNP denotes Boston
T9053 8914-8916 , denotes ,
T9054 8916-8918 NNP denotes MA
T9055 8918-8919 -RRB- denotes )
T9056 8920-8923 CC denotes and
T9057 8924-8931 VBN denotes exposed
T9058 8932-8934 IN denotes to
T9059 8935-8940 NN denotes X-ray
T9060 8941-8945 NN denotes film
T9061 8945-8946 . denotes .
T9141 8948-8959 JJ denotes Statistical
T9142 8960-8968 NNS denotes analyses
T9143 8968-9079 sentence denotes All BCAA and BCKDH enzyme activity data are presented as the mean +/- the standard error of the mean (S.E.M.).
T9144 8969-8972 DT denotes All
T9145 9004-9008 NNS denotes data
T9146 8973-8977 NN denotes BCAA
T9147 8978-8981 CC denotes and
T9148 8982-8987 NN denotes BCKDH
T9149 8988-8994 NN denotes enzyme
T9150 8995-9003 NN denotes activity
T9151 9013-9022 VBN denotes presented
T9152 9009-9012 VBP denotes are
T9153 9023-9025 IN denotes as
T9154 9026-9029 DT denotes the
T9155 9030-9034 NN denotes mean
T9156 9035-9036 SYM denotes +
T9157 9036-9037 HYPH denotes /
T9158 9037-9038 SYM denotes -
T9159 9039-9042 DT denotes the
T9160 9052-9057 NN denotes error
T9161 9043-9051 JJ denotes standard
T9162 9058-9060 IN denotes of
T9163 9061-9064 DT denotes the
T9164 9065-9069 NN denotes mean
T9165 9070-9071 -LRB- denotes (
T9166 9071-9077 NN denotes S.E.M.
T9167 9077-9078 -RRB- denotes )
T9168 9078-9079 . denotes .
T9169 9079-9148 sentence denotes Differences between genotypes were compared by Student's t test[32].
T9170 9080-9091 NNS denotes Differences
T9171 9115-9123 VBN denotes compared
T9172 9092-9099 IN denotes between
T9173 9100-9109 NNS denotes genotypes
T9174 9110-9114 VBD denotes were
T9175 9124-9126 IN denotes by
T9176 9127-9134 NNP denotes Student
T9177 9139-9143 NN denotes test
T9178 9134-9136 POS denotes 's
T9179 9137-9138 NN denotes t
T9180 9143-9144 -LRB- denotes [
T9181 9144-9146 CD denotes 32
T9182 9146-9147 -RRB- denotes ]
T9183 9147-9148 . denotes .
T22055 1612-1614 NN denotes E2
T22056 1635-1645 NN denotes production
T22057 1615-1619 NN denotes gene
T22058 1620-1628 NN denotes knockout
T22059 1629-1634 NN denotes mouse
T22060 1645-1646 . denotes .
T22061 1646-1724 sentence denotes A, Gene targeting strategy used for targeting the E2 locus in mouse ES cells.
T22062 1647-1648 LS denotes A
T22063 1665-1673 NN denotes strategy
T22064 1648-1650 , denotes ,
T22065 1650-1654 NN denotes Gene
T22066 1655-1664 NN denotes targeting
T22067 1674-1678 VBN denotes used
T22068 1679-1682 IN denotes for
T22069 1683-1692 VBG denotes targeting
T22070 1693-1696 DT denotes the
T22071 1700-1705 NN denotes locus
T22072 1697-1699 NN denotes E2
T22073 1706-1708 IN denotes in
T22074 1709-1714 NN denotes mouse
T22075 1718-1723 NNS denotes cells
T22076 1715-1717 NN denotes ES
T22077 1723-1724 . denotes .
T22078 1724-1852 sentence denotes The targeting construct was designed to delete 1.67 kb of sequence between an EcoRV site in Exon 4 and a Smal site in intron 5.
T22079 1725-1728 DT denotes The
T22080 1739-1748 NN denotes construct
T22081 1729-1738 NN denotes targeting
T22082 1753-1761 VBN denotes designed
T22083 1749-1752 VBD denotes was
T22084 1762-1764 TO denotes to
T22085 1765-1771 VB denotes delete
T22086 1772-1776 CD denotes 1.67
T22087 1777-1779 NN denotes kb
T22088 1780-1782 IN denotes of
T22089 1783-1791 NN denotes sequence
T22090 1792-1799 IN denotes between
T22091 1800-1802 DT denotes an
T22092 1809-1813 NN denotes site
T22093 1803-1808 NN denotes EcoRV
T22094 1814-1816 IN denotes in
T22095 1817-1821 NN denotes Exon
T22096 1822-1823 CD denotes 4
T22097 1824-1827 CC denotes and
T22098 1828-1829 DT denotes a
T22099 1835-1839 NN denotes site
T22100 1830-1834 NN denotes Smal
T22101 1840-1842 IN denotes in
T22102 1843-1849 NN denotes intron
T22103 1850-1851 CD denotes 5
T22104 1851-1852 . denotes .
T22105 1852-1965 sentence denotes The wild type E2 gene contains an ~16 kb BglI restriction fragment that hybridizes to the Exon 6 specific probe.
T22106 1853-1856 DT denotes The
T22107 1870-1874 NN denotes gene
T22108 1857-1861 JJ denotes wild
T22109 1862-1866 NN denotes type
T22110 1867-1869 NN denotes E2
T22111 1875-1883 VBZ denotes contains
T22112 1884-1886 DT denotes an
T22113 1911-1919 NN denotes fragment
T22114 1887-1888 SYM denotes ~
T22115 1888-1890 CD denotes 16
T22116 1891-1893 NN denotes kb
T22117 1894-1898 NN denotes BglI
T22118 1899-1910 NN denotes restriction
T22119 1920-1924 WDT denotes that
T22120 1925-1935 VBZ denotes hybridizes
T22121 1936-1938 IN denotes to
T22122 1939-1942 DT denotes the
T22123 1959-1964 NN denotes probe
T22124 1943-1947 NN denotes Exon
T22125 1950-1958 JJ denotes specific
T22126 1948-1949 CD denotes 6
T22127 1964-1965 . denotes .
T22128 1965-2074 sentence denotes A correctly targeted E2 locus harbors an ~11 kb BglI restriction fragment that hybridizes to the same probe.
T22129 1966-1967 DT denotes A
T22130 1990-1995 NN denotes locus
T22131 1968-1977 RB denotes correctly
T22132 1978-1986 VBN denotes targeted
T22133 1987-1989 NN denotes E2
T22134 1996-2003 VBZ denotes harbors
T22135 2004-2006 DT denotes an
T22136 2031-2039 NN denotes fragment
T22137 2007-2008 SYM denotes ~
T22138 2008-2010 CD denotes 11
T22139 2011-2013 NN denotes kb
T22140 2014-2018 NN denotes BglI
T22141 2019-2030 NN denotes restriction
T22142 2040-2044 WDT denotes that
T22143 2045-2055 VBZ denotes hybridizes
T22144 2056-2058 IN denotes to
T22145 2059-2062 DT denotes the
T22146 2068-2073 NN denotes probe
T22147 2063-2067 JJ denotes same
T22148 2073-2074 . denotes .
T22149 2074-2201 sentence denotes Note that the probe will not detect random integration of the targeting vector because it is external to the targeting vector.
T22150 2075-2079 VB denotes Note
T22151 2080-2084 IN denotes that
T22152 2104-2110 VB denotes detect
T22153 2085-2088 DT denotes the
T22154 2089-2094 NN denotes probe
T22155 2095-2099 MD denotes will
T22156 2100-2103 RB denotes not
T22157 2111-2117 JJ denotes random
T22158 2118-2129 NN denotes integration
T22159 2130-2132 IN denotes of
T22160 2133-2136 DT denotes the
T22161 2147-2153 NN denotes vector
T22162 2137-2146 NN denotes targeting
T22163 2154-2161 IN denotes because
T22164 2165-2167 VBZ denotes is
T22165 2162-2164 PRP denotes it
T22166 2168-2176 JJ denotes external
T22167 2177-2179 IN denotes to
T22168 2180-2183 DT denotes the
T22169 2194-2200 NN denotes vector
T22170 2184-2193 NN denotes targeting
T22171 2200-2201 . denotes .
T22172 2201-2436 sentence denotes B, Southern blot analysis of Bgll digested genomic DNA derived from the parental wild type ES cell line (R1), a heterozygous targeted ES cell line (362), and from wild type (+/+), heterozygous (+/-) and homozygous knockout (-/-) mice.
T22173 2202-2203 LS denotes B
T22174 2219-2227 NN denotes analysis
T22175 2203-2205 , denotes ,
T22176 2205-2213 NNP denotes Southern
T22177 2214-2218 NN denotes blot
T22178 2228-2230 IN denotes of
T22179 2231-2235 NN denotes Bgll
T22180 2236-2244 VBN denotes digested
T22181 2253-2256 NN denotes DNA
T22182 2245-2252 JJ denotes genomic
T22183 2257-2264 VBN denotes derived
T22184 2265-2269 IN denotes from
T22185 2270-2273 DT denotes the
T22186 2301-2305 NN denotes line
T22187 2274-2282 JJ denotes parental
T22188 2283-2287 JJ denotes wild
T22189 2288-2292 NN denotes type
T22190 2293-2295 NN denotes ES
T22191 2296-2300 NN denotes cell
T22192 2306-2307 -LRB- denotes (
T22193 2307-2309 NN denotes R1
T22194 2309-2310 -RRB- denotes )
T22195 2310-2312 , denotes ,
T22196 2312-2313 DT denotes a
T22197 2344-2348 NN denotes line
T22198 2314-2326 JJ denotes heterozygous
T22199 2327-2335 VBN denotes targeted
T22200 2336-2338 NN denotes ES
T22201 2339-2343 NN denotes cell
T22202 2349-2350 -LRB- denotes (
T22203 2350-2353 CD denotes 362
T22204 2353-2354 -RRB- denotes )
T22205 2354-2356 , denotes ,
T22206 2356-2359 CC denotes and
T22207 2360-2364 IN denotes from
T22208 2365-2369 JJ denotes wild
T22209 2370-2374 NN denotes type
T22210 2416-2424 NN denotes knockout
T22211 2375-2376 -LRB- denotes (
T22212 2378-2379 SYM denotes +
T22213 2376-2377 SYM denotes +
T22214 2377-2378 HYPH denotes /
T22215 2379-2380 -RRB- denotes )
T22216 2380-2382 , denotes ,
T22217 2382-2394 JJ denotes heterozygous
T22218 2395-2396 -LRB- denotes (
T22219 2398-2399 SYM denotes -
T22220 2396-2397 SYM denotes +
T22221 2397-2398 HYPH denotes /
T22222 2399-2400 -RRB- denotes )
T22223 2401-2404 CC denotes and
T22224 2405-2415 JJ denotes homozygous
T22225 2431-2435 NNS denotes mice
T22226 2425-2426 -LRB- denotes (
T22227 2428-2429 SYM denotes -
T22228 2426-2427 SYM denotes -
T22229 2427-2428 HYPH denotes /
T22230 2429-2430 -RRB- denotes )
T22231 2435-2436 . denotes .
T22232 2436-2491 sentence denotes The blot was hybridized with an Exon 6 specific probe.
T22233 2437-2440 DT denotes The
T22234 2441-2445 NN denotes blot
T22235 2450-2460 VBN denotes hybridized
T22236 2446-2449 VBD denotes was
T22237 2461-2465 IN denotes with
T22238 2466-2468 DT denotes an
T22239 2485-2490 NN denotes probe
T22240 2469-2473 NN denotes Exon
T22241 2476-2484 JJ denotes specific
T22242 2474-2475 CD denotes 6
T22243 2490-2491 . denotes .
T22244 2491-2623 sentence denotes C, Immunohistochemical analysis of fresh frozen liver sections from control (+/+) and E2 knockout (-/-) postnatal day 1 mouse pups.
T22245 2492-2493 LS denotes C
T22246 2515-2523 NN denotes analysis
T22247 2493-2495 , denotes ,
T22248 2495-2514 JJ denotes Immunohistochemical
T22249 2524-2526 IN denotes of
T22250 2527-2532 JJ denotes fresh
T22251 2533-2539 VBN denotes frozen
T22252 2546-2554 NNS denotes sections
T22253 2540-2545 NN denotes liver
T22254 2555-2559 IN denotes from
T22255 2560-2567 NN denotes control
T22256 2581-2589 NN denotes knockout
T22257 2568-2569 -LRB- denotes (
T22258 2571-2572 SYM denotes +
T22259 2569-2570 SYM denotes +
T22260 2570-2571 HYPH denotes /
T22261 2572-2573 -RRB- denotes )
T22262 2574-2577 CC denotes and
T22263 2578-2580 NN denotes E2
T22264 2618-2622 NNS denotes pups
T22265 2590-2591 -LRB- denotes (
T22266 2593-2594 SYM denotes -
T22267 2591-2592 SYM denotes -
T22268 2592-2593 HYPH denotes /
T22269 2594-2595 -RRB- denotes )
T22270 2596-2605 JJ denotes postnatal
T22271 2606-2609 NN denotes day
T22272 2610-2611 CD denotes 1
T22273 2612-2617 NN denotes mouse
T22274 2622-2623 . denotes .
T22275 2623-2718 sentence denotes Sections were stained for E2 using an E2 specific antibody (green) and a nuclear stain (blue).
T22276 2624-2632 NNS denotes Sections
T22277 2638-2645 VBN denotes stained
T22278 2633-2637 VBD denotes were
T22279 2646-2649 IN denotes for
T22280 2650-2652 NN denotes E2
T22281 2653-2658 VBG denotes using
T22282 2659-2661 DT denotes an
T22283 2674-2682 NN denotes antibody
T22284 2662-2664 NN denotes E2
T22285 2665-2673 JJ denotes specific
T22286 2683-2684 -LRB- denotes (
T22287 2684-2689 JJ denotes green
T22288 2689-2690 -RRB- denotes )
T22289 2691-2694 CC denotes and
T22290 2695-2696 DT denotes a
T22291 2705-2710 NN denotes stain
T22292 2697-2704 JJ denotes nuclear
T22293 2711-2712 -LRB- denotes (
T22294 2712-2716 JJ denotes blue
T22295 2716-2717 -RRB- denotes )
T22296 2717-2718 . denotes .
T22297 2718-2807 sentence denotes Note the complete absence of E2 immunoreactivity in the section from the knockout mouse.
T22298 2719-2723 VB denotes Note
T22299 2724-2727 DT denotes the
T22300 2737-2744 NN denotes absence
T22301 2728-2736 JJ denotes complete
T22302 2745-2747 IN denotes of
T22303 2748-2750 NN denotes E2
T22304 2751-2767 NN denotes immunoreactivity
T22305 2768-2770 IN denotes in
T22306 2771-2774 DT denotes the
T22307 2775-2782 NN denotes section
T22308 2783-2787 IN denotes from
T22309 2788-2791 DT denotes the
T22310 2801-2806 NN denotes mouse
T22311 2792-2800 NN denotes knockout
T22312 2806-2807 . denotes .
T22313 2807-2921 sentence denotes D, Similar results were observed upon immunohistochemical analysis of primary mouse embryonic fibroblasts (MEFs).
T22314 2808-2809 LS denotes D
T22315 2832-2840 VBN denotes observed
T22316 2809-2811 , denotes ,
T22317 2811-2818 JJ denotes Similar
T22318 2819-2826 NNS denotes results
T22319 2827-2831 VBD denotes were
T22320 2841-2845 IN denotes upon
T22321 2846-2865 JJ denotes immunohistochemical
T22322 2866-2874 NN denotes analysis
T22323 2875-2877 IN denotes of
T22324 2878-2885 JJ denotes primary
T22325 2902-2913 NNS denotes fibroblasts
T22326 2886-2891 NN denotes mouse
T22327 2892-2901 JJ denotes embryonic
T22328 2914-2915 -LRB- denotes (
T22329 2915-2919 NNS denotes MEFs
T22330 2919-2920 -RRB- denotes )
T22331 2920-2921 . denotes .
T22332 2921-3081 sentence denotes Note that the readily detectable signal for E2 in the control cells was present in a pattern characteristic of mitochondria, the subcellular location of BCKDH.
T22333 2922-2926 VB denotes Note
T22334 2927-2931 IN denotes that
T22335 2990-2993 VBD denotes was
T22336 2932-2935 DT denotes the
T22337 2955-2961 NN denotes signal
T22338 2936-2943 RB denotes readily
T22339 2944-2954 JJ denotes detectable
T22340 2962-2965 IN denotes for
T22341 2966-2968 NN denotes E2
T22342 2969-2971 IN denotes in
T22343 2972-2975 DT denotes the
T22344 2984-2989 NNS denotes cells
T22345 2976-2983 NN denotes control
T22346 2994-3001 JJ denotes present
T22347 3002-3004 IN denotes in
T22348 3005-3006 DT denotes a
T22349 3007-3014 NN denotes pattern
T22350 3015-3029 JJ denotes characteristic
T22351 3030-3032 IN denotes of
T22352 3033-3045 NNS denotes mitochondria
T22353 3045-3047 , denotes ,
T22354 3047-3050 DT denotes the
T22355 3063-3071 NN denotes location
T22356 3051-3062 JJ denotes subcellular
T22357 3072-3074 IN denotes of
T22358 3075-3080 NN denotes BCKDH
T22359 3080-3081 . denotes .
R1293 T4359 T4360 det All,studies
R1294 T4360 T4361 nsubjpass studies,reviewed
R1295 T4362 T4360 acl involving,studies
R1296 T4363 T4362 dobj animals,involving
R1297 T4364 T4361 auxpass were,reviewed
R1298 T4365 T4361 cc and,reviewed
R1299 T4366 T4361 conj approved,reviewed
R1300 T4367 T4366 agent by,approved
R1301 T4368 T4369 det the,University
R1302 T4369 T4367 pobj University,by
R1303 T4370 T4369 prep of,University
R1304 T4371 T4372 poss Pittsburgh,Committee
R1305 T4372 T4370 pobj Committee,of
R1306 T4373 T4371 case 's,Pittsburgh
R1307 T4374 T4375 nmod Institutional,Animal
R1308 T4375 T4376 nmod Animal,Care
R1309 T4376 T4372 nmod Care,Committee
R1310 T4377 T4376 cc and,Care
R1311 T4378 T4376 conj Use,Care
R1312 T4379 T4361 punct .,reviewed
R1313 T5027 T5028 compound Gene,knockout
R1314 T5028 T5029 compound knockout,production
R1315 T5031 T5032 det A,subclone
R1316 T5032 T5035 nsubjpass subclone,obtained
R1317 T5033 T5032 amod genomic,subclone
R1318 T5034 T5032 compound DNA,subclone
R1319 T5036 T5032 prep of,subclone
R1320 T5037 T5038 nmod Strain,DNA
R1321 T5038 T5036 pobj DNA,of
R1322 T5039 T5040 nummod 129,SvJ
R1323 T5040 T5038 compound SvJ,DNA
R1324 T5041 T5040 punct /,SvJ
R1325 T5042 T5043 dep that,contained
R1326 T5043 T5032 relcl contained,subclone
R1327 T5044 T5045 det a,portion
R1328 T5045 T5043 dobj portion,contained
R1329 T5046 T5045 prep of,portion
R1330 T5047 T5048 nmod Exons,4
R1331 T5048 T5046 pobj 4,of
R1332 T5049 T5048 cc and,4
R1333 T5050 T5048 conj 5,4
R1334 T5051 T5048 prep of,4
R1335 T5052 T5053 det the,gene
R1336 T5053 T5051 pobj gene,of
R1337 T5054 T5053 compound E2,gene
R1338 T5055 T5053 cc and,gene
R1339 T5056 T5057 compound flanking,DNA
R1340 T5057 T5053 conj DNA,gene
R1341 T5058 T5035 auxpass was,obtained
R1342 T5059 T5035 prep from,obtained
R1343 T5060 T5061 det a,library
R1344 T5061 T5059 pobj library,from
R1345 T5062 T5063 compound P1,phage
R1346 T5063 T5061 compound phage,library
R1347 T5064 T5061 prep from,library
R1348 T5065 T5066 nmod Genome,Inc.
R1349 T5066 T5064 pobj Inc.,from
R1350 T5067 T5066 nmod Systems,Inc.
R1351 T5068 T5066 punct ", ",Inc.
R1352 T5069 T5066 punct ", ",Inc.
R1353 T5070 T5071 punct (,Library
R1354 T5071 T5066 poss Library,Inc.
R1355 T5072 T5073 compound St.,Louis
R1356 T5073 T5071 dep Louis,Library
R1357 T5074 T5073 punct ", ",Louis
R1358 T5075 T5073 npadvmod MO,Louis
R1359 T5076 T5071 punct ;,Library
R1360 T5077 T5071 punct ',Library
R1361 T5078 T5079 nummod 3,Hit
R1362 T5079 T5071 compound Hit,Library
R1363 T5080 T5079 punct -,Hit
R1364 T5081 T5082 compound Mouse,ES
R1365 T5082 T5071 compound ES,Library
R1366 T5083 T5071 dep ',Library
R1367 T5084 T5071 punct ),Library
R1368 T5085 T5035 punct .,obtained
R1369 T5087 T5088 det A,vector
R1370 T5088 T5096 nsubjpass vector,created
R1371 T5089 T5090 amod positive,negative
R1372 T5090 T5088 amod negative,vector
R1373 T5091 T5090 punct /,negative
R1374 T5092 T5093 compound replacement,type
R1375 T5093 T5088 compound type,vector
R1376 T5094 T5095 compound gene,targeting
R1377 T5095 T5088 compound targeting,vector
R1378 T5097 T5098 punct [,21
R1379 T5098 T5088 parataxis 21,vector
R1380 T5099 T5098 punct ],21
R1381 T5100 T5096 auxpass was,created
R1382 T5101 T5096 prep by,created
R1383 T5102 T5101 pcomp replacing,by
R1384 T5103 T5104 det a,fragment
R1385 T5104 T5102 dobj fragment,replacing
R1386 T5105 T5106 nummod 1.67,kb
R1387 T5106 T5104 compound kb,fragment
R1388 T5107 T5108 compound EcoRV,Smal
R1389 T5108 T5104 compound Smal,fragment
R1390 T5109 T5108 punct -,Smal
R1391 T5110 T5111 dep that,included
R1392 T5111 T5104 relcl included,fragment
R1393 T5112 T5113 det a,portion
R1394 T5113 T5111 dobj portion,included
R1395 T5114 T5113 prep of,portion
R1396 T5115 T5114 pobj Exon,of
R1397 T5116 T5115 nummod 4,Exon
R1398 T5117 T5113 cc and,portion
R1399 T5118 T5113 conj all,portion
R1400 T5119 T5118 prep of,all
R1401 T5120 T5119 pobj Exon,of
R1402 T5121 T5120 nummod 5,Exon
R1403 T5122 T5113 prep with,portion
R1404 T5123 T5124 det the,gene
R1405 T5124 T5122 pobj gene,with
R1406 T5125 T5124 compound PGKneo,gene
R1407 T5126 T5124 compound marker,gene
R1408 T5127 T5128 punct (,See
R1409 T5128 T5124 parataxis See,gene
R1410 T5129 T5130 compound Fig.,1A
R1411 T5130 T5128 dobj 1A,See
R1412 T5131 T5128 punct ),See
R1413 T5132 T5124 prep from,gene
R1414 T5133 T5134 det the,vector
R1415 T5134 T5132 pobj vector,from
R1416 T5135 T5134 compound pPNT,vector
R1417 T5136 T5137 punct [,22
R1418 T5137 T5111 parataxis 22,included
R1419 T5138 T5137 punct ],22
R1420 T5139 T5096 punct .,created
R1421 T5141 T5142 det The,construct
R1422 T5142 T5144 nsubjpass construct,linearized
R1423 T5143 T5142 compound targeting,construct
R1424 T5145 T5144 auxpass was,linearized
R1425 T5146 T5144 prep with,linearized
R1426 T5147 T5146 pobj NotI,with
R1427 T5148 T5144 cc and,linearized
R1428 T5149 T5144 conj electroporated,linearized
R1429 T5150 T5149 prep into,electroporated
R1430 T5151 T5152 compound R1,cells
R1431 T5152 T5150 pobj cells,into
R1432 T5153 T5152 compound ES,cells
R1433 T5154 T5155 punct [,23
R1434 T5155 T5152 parataxis 23,cells
R1435 T5156 T5155 punct ],23
R1436 T5157 T5149 prep under,electroporated
R1437 T5158 T5157 pobj conditions,under
R1438 T5159 T5158 acl described,conditions
R1439 T5160 T5159 advmod previously,described
R1440 T5161 T5162 punct [,24
R1441 T5162 T5159 parataxis 24,described
R1442 T5163 T5162 punct ],24
R1443 T5164 T5144 punct .,linearized
R1444 T5166 T5167 compound ES,cells
R1445 T5167 T5168 nsubjpass cells,selected
R1446 T5169 T5168 auxpass were,selected
R1447 T5170 T5168 prep with,selected
R1448 T5171 T5170 pobj G418,with
R1449 T5172 T5173 punct (,Inc.
R1450 T5173 T5171 parataxis Inc.,G418
R1451 T5174 T5175 nummod 300,μg
R1452 T5175 T5173 dep μg,Inc.
R1453 T5176 T5177 punct /,ml
R1454 T5177 T5175 prep ml,μg
R1455 T5178 T5173 punct ;,Inc.
R1456 T5179 T5173 compound Life,Inc.
R1457 T5180 T5173 compound Technologies,Inc.
R1458 T5181 T5173 punct ", ",Inc.
R1459 T5182 T5173 npadvmod Gaithersburg,Inc.
R1460 T5183 T5173 punct ", ",Inc.
R1461 T5184 T5173 npadvmod MD,Inc.
R1462 T5185 T5173 punct ),Inc.
R1463 T5186 T5171 cc and,G418
R1464 T5187 T5171 conj gancyclovir,G418
R1465 T5188 T5187 punct (,gancyclovir
R1466 T5189 T5190 nummod 2,μM
R1467 T5190 T5187 nmod μM,gancyclovir
R1468 T5191 T5187 punct ;,gancyclovir
R1469 T5192 T5187 nmod gift,gancyclovir
R1470 T5193 T5192 prep of,gift
R1471 T5194 T5193 pobj Syntex,of
R1472 T5195 T5194 punct ", ",Syntex
R1473 T5196 T5197 compound Palo,Alto
R1474 T5197 T5194 npadvmod Alto,Syntex
R1475 T5198 T5194 punct ", ",Syntex
R1476 T5199 T5194 npadvmod CA,Syntex
R1477 T5200 T5187 punct ),gancyclovir
R1478 T5201 T5168 punct .,selected
R1479 T5203 T5204 advmod Doubly,resistant
R1480 T5204 T5205 amod resistant,clones
R1481 T5205 T5206 nsubjpass clones,screened
R1482 T5207 T5206 auxpass were,screened
R1483 T5208 T5206 prep for,screened
R1484 T5209 T5210 compound gene,targeting
R1485 T5210 T5208 pobj targeting,for
R1486 T5211 T5206 prep by,screened
R1487 T5212 T5213 compound Southern,blot
R1488 T5213 T5214 compound blot,analysis
R1489 T5214 T5211 pobj analysis,by
R1490 T5215 T5214 prep of,analysis
R1491 T5216 T5217 nmod BglI,DNA
R1492 T5217 T5215 pobj DNA,of
R1493 T5218 T5217 amod digested,DNA
R1494 T5219 T5217 amod genomic,DNA
R1495 T5220 T5206 punct .,screened
R1496 T5222 T5223 nsubjpass Blots,hybridized
R1497 T5224 T5223 auxpass were,hybridized
R1498 T5225 T5223 prep with,hybridized
R1499 T5226 T5227 det an,probe
R1500 T5227 T5225 pobj probe,with
R1501 T5228 T5229 npadvmod Exon,specific
R1502 T5229 T5227 amod specific,probe
R1503 T5230 T5228 nummod 6,Exon
R1504 T5231 T5232 dep that,was
R1505 T5232 T5227 relcl was,probe
R1506 T5233 T5232 acomp external,was
R1507 T5234 T5233 prep to,external
R1508 T5235 T5236 det the,construct
R1509 T5236 T5234 pobj construct,to
R1510 T5237 T5238 compound gene,targeting
R1511 T5238 T5236 compound targeting,construct
R1512 T5239 T5223 punct .,hybridized
R1513 T5241 T5242 advmod Correctly,targeted
R1514 T5242 T5243 amod targeted,cells
R1515 T5243 T5245 nsubjpass cells,injected
R1516 T5244 T5243 compound ES,cells
R1517 T5246 T5245 auxpass were,injected
R1518 T5247 T5245 prep into,injected
R1519 T5248 T5249 compound C57BL,6J
R1520 T5249 T5251 compound 6J,blastocysts
R1521 T5250 T5249 punct /,6J
R1522 T5251 T5247 pobj blastocysts,into
R1523 T5252 T5253 aux to,produce
R1524 T5253 T5245 advcl produce,injected
R1525 T5254 T5255 amod chimeric,mice
R1526 T5255 T5253 dobj mice,produce
R1527 T5256 T5253 advcl using,produce
R1528 T5257 T5258 amod standard,procedures
R1529 T5258 T5256 dobj procedures,using
R1530 T5259 T5245 punct .,injected
R1531 T5261 T5262 amod Heterozygous,offspring
R1532 T5262 T5263 nsubjpass offspring,intercrossed
R1533 T5264 T5262 prep from,offspring
R1534 T5265 T5266 npadvmod germline,competent
R1535 T5266 T5267 amod competent,chimeras
R1536 T5267 T5264 pobj chimeras,from
R1537 T5268 T5263 auxpass were,intercrossed
R1538 T5269 T5270 aux to,produce
R1539 T5270 T5263 advcl produce,intercrossed
R1540 T5271 T5272 amod wild,type
R1541 T5272 T5273 nmod type,knockout
R1542 T5273 T5288 nmod knockout,mice
R1543 T5274 T5275 punct (,+
R1544 T5275 T5272 punct +,type
R1545 T5276 T5275 punct +,+
R1546 T5277 T5275 punct /,+
R1547 T5278 T5275 punct ),+
R1548 T5279 T5272 punct ", ",type
R1549 T5280 T5272 conj heterozygous,type
R1550 T5281 T5282 punct (,-
R1551 T5282 T5280 punct -,heterozygous
R1552 T5283 T5282 punct +,-
R1553 T5284 T5282 punct /,-
R1554 T5285 T5282 punct ),-
R1555 T5286 T5280 cc and,heterozygous
R1556 T5287 T5280 conj homozygous,heterozygous
R1557 T5288 T5270 dobj mice,produce
R1558 T5289 T5290 punct (,-
R1559 T5290 T5273 punct -,knockout
R1560 T5291 T5290 punct -,-
R1561 T5292 T5290 punct /,-
R1562 T5293 T5290 punct ),-
R1563 T5294 T5263 punct .,intercrossed
R1564 T5296 T5297 prep At,used
R1565 T5298 T5299 det all,generations
R1566 T5299 T5296 pobj generations,At
R1567 T5300 T5297 punct ", ",used
R1568 T5301 T5302 punct +,-
R1569 T5302 T5304 punct -,pairs
R1570 T5303 T5302 punct /,-
R1571 T5304 T5297 nsubjpass pairs,used
R1572 T5305 T5304 amod breeding,pairs
R1573 T5306 T5297 auxpass were,used
R1574 T5307 T5297 punct .,used
R1575 T5309 T5310 nsubj Results,are
R1576 T5311 T5309 acl presented,Results
R1577 T5312 T5311 advmod here,presented
R1578 T5313 T5310 prep from,are
R1579 T5314 T5313 pobj mice,from
R1580 T5315 T5314 acl derived,mice
R1581 T5316 T5315 prep from,derived
R1582 T5317 T5318 det the,generations
R1583 T5318 T5316 pobj generations,from
R1584 T5319 T5318 nmod F2,generations
R1585 T5320 T5319 punct +,F2
R1586 T5321 T5310 punct .,are
R1587 T5323 T5324 det All,animals
R1588 T5324 T5325 nsubj animals,were
R1589 T5326 T5325 prep of,were
R1590 T5327 T5328 det a,background
R1591 T5328 T5326 pobj background,of
R1592 T5329 T5328 amod mixed,background
R1593 T5330 T5331 nmod C57BL,6J
R1594 T5331 T5328 nmod 6J,background
R1595 T5332 T5331 punct /,6J
R1596 T5333 T5331 punct ×,6J
R1597 T5334 T5335 compound Strain,SvJ
R1598 T5335 T5331 appos SvJ,6J
R1599 T5336 T5335 compound 129Sv,SvJ
R1600 T5337 T5335 punct /,SvJ
R1601 T5338 T5328 amod genetic,background
R1602 T5339 T5325 punct .,were
R1603 T5873 T5872 prep of,Production
R1604 T5874 T5875 amod transgenic,mice
R1605 T5875 T5873 pobj mice,of
R1606 T5877 T5878 amod Standard,techniques
R1607 T5878 T5880 nsubjpass techniques,used
R1608 T5879 T5878 amod molecular,techniques
R1609 T5881 T5880 auxpass were,used
R1610 T5882 T5883 aux to,assemble
R1611 T5883 T5880 advcl assemble,used
R1612 T5884 T5885 det the,construct
R1613 T5885 T5883 dobj construct,assemble
R1614 T5886 T5885 amod transgenic,construct
R1615 T5887 T5885 punct ", ",construct
R1616 T5888 T5889 compound pTRE,E2
R1617 T5889 T5885 appos E2,construct
R1618 T5890 T5889 punct -,E2
R1619 T5891 T5880 punct .,used
R1620 T5893 T5894 det This,transgene
R1621 T5894 T5895 nsubj transgene,contains
R1622 T5896 T5897 det the,promoter
R1623 T5897 T5895 dobj promoter,contains
R1624 T5898 T5897 nmod tetracycline,promoter
R1625 T5899 T5897 amod responsive,promoter
R1626 T5900 T5897 nmod hCMV,promoter
R1627 T5901 T5900 punct *,hCMV
R1628 T5902 T5900 punct -,hCMV
R1629 T5903 T5900 nummod 1,hCMV
R1630 T5904 T5897 punct [,promoter
R1631 T5905 T5897 acl consisting,promoter
R1632 T5906 T5905 prep of,consisting
R1633 T5907 T5908 det the,element
R1634 T5908 T5906 pobj element,of
R1635 T5909 T5908 nmod tetracycline,element
R1636 T5910 T5908 amod responsive,element
R1637 T5911 T5908 punct (,element
R1638 T5912 T5908 appos TRE,element
R1639 T5913 T5908 punct ),element
R1640 T5914 T5908 cc and,element
R1641 T5915 T5916 det a,promoter
R1642 T5916 T5908 conj promoter,element
R1643 T5917 T5916 amod minimal,promoter
R1644 T5918 T5916 compound hCMV,promoter
R1645 T5919 T5916 punct ],promoter
R1646 T5920 T5921 punct [,25
R1647 T5921 T5916 parataxis 25,promoter
R1648 T5922 T5921 punct ],25
R1649 T5923 T5916 prep from,promoter
R1650 T5924 T5923 pobj pTRE2,from
R1651 T5925 T5926 punct (,Inc.
R1652 T5926 T5916 parataxis Inc.,promoter
R1653 T5927 T5926 compound Clontech,Inc.
R1654 T5928 T5926 punct ", ",Inc.
R1655 T5929 T5930 compound Mt.,View
R1656 T5930 T5926 npadvmod View,Inc.
R1657 T5931 T5926 punct ", ",Inc.
R1658 T5932 T5926 npadvmod CA,Inc.
R1659 T5933 T5926 punct ),Inc.
R1660 T5934 T5897 punct ", ",promoter
R1661 T5935 T5936 det a,intron
R1662 T5936 T5897 conj intron,promoter
R1663 T5937 T5936 amod chimeric,intron
R1664 T5938 T5936 prep from,intron
R1665 T5939 T5938 pobj pCI,from
R1666 T5940 T5941 punct (,Promega
R1667 T5941 T5936 parataxis Promega,intron
R1668 T5942 T5941 punct ),Promega
R1669 T5943 T5944 aux to,increase
R1670 T5944 T5936 advcl increase,intron
R1671 T5945 T5946 compound message,stability
R1672 T5946 T5944 dobj stability,increase
R1673 T5947 T5946 cc and,stability
R1674 T5948 T5946 conj expression,stability
R1675 T5949 T5950 punct [,27
R1676 T5950 T5944 parataxis 27,increase
R1677 T5951 T5950 nummod 26,27
R1678 T5952 T5950 punct ",",27
R1679 T5953 T5950 punct ],27
R1680 T5954 T5936 punct ", ",intron
R1681 T5955 T5956 det a,sequence
R1682 T5956 T5936 conj sequence,intron
R1683 T5957 T5956 compound Kozak,sequence
R1684 T5958 T5956 compound consensus,sequence
R1685 T5959 T5956 prep at,sequence
R1686 T5960 T5961 det the,codon
R1687 T5961 T5959 pobj codon,at
R1688 T5962 T5961 compound initiation,codon
R1689 T5963 T5964 aux to,optimize
R1690 T5964 T5956 advcl optimize,sequence
R1691 T5965 T5964 dobj translation,optimize
R1692 T5966 T5967 punct [,28
R1693 T5967 T5964 parataxis 28,optimize
R1694 T5968 T5967 punct ],28
R1695 T5969 T5956 punct ", ",sequence
R1696 T5970 T5971 det the,cDNA
R1697 T5971 T5956 conj cDNA,sequence
R1698 T5972 T5971 amod human,cDNA
R1699 T5973 T5971 compound E2,cDNA
R1700 T5974 T5975 dep which,modified
R1701 T5975 T5971 relcl modified,cDNA
R1702 T5976 T5975 aux has,modified
R1703 T5977 T5975 auxpass been,modified
R1704 T5978 T5979 aux to,contain
R1705 T5979 T5975 advcl contain,modified
R1706 T5980 T5981 det a,linker
R1707 T5981 T5979 dobj linker,contain
R1708 T5982 T5981 nummod 4,linker
R1709 T5983 T5982 punct ×,4
R1710 T5984 T5981 compound alanine,linker
R1711 T5985 T5981 acl followed,linker
R1712 T5986 T5985 agent by,followed
R1713 T5987 T5988 det a,tag
R1714 T5988 T5986 pobj tag,by
R1715 T5989 T5990 compound c,myc
R1716 T5990 T5988 compound myc,tag
R1717 T5991 T5990 punct -,myc
R1718 T5992 T5988 compound epitope,tag
R1719 T5993 T5988 prep at,tag
R1720 T5994 T5995 det the,terminus
R1721 T5995 T5993 pobj terminus,at
R1722 T5996 T5995 compound carboxy,terminus
R1723 T5997 T5998 aux to,facilitate
R1724 T5998 T5988 advcl facilitate,tag
R1725 T5999 T5998 dobj detection,facilitate
R1726 T6000 T5971 punct ", ",cDNA
R1727 T6001 T5971 cc and,cDNA
R1728 T6002 T6003 det an,sequence
R1729 T6003 T5971 conj sequence,cDNA
R1730 T6004 T6003 nmod SV40,sequence
R1731 T6005 T6003 amod late,sequence
R1732 T6006 T6003 compound polyadenylation,sequence
R1733 T6007 T6003 prep from,sequence
R1734 T6008 T6007 pobj pCI,from
R1735 T6009 T6003 prep for,sequence
R1736 T6010 T6011 amod enhanced,stability
R1737 T6011 T6009 pobj stability,for
R1738 T6012 T6011 compound mRNA,stability
R1739 T6013 T6011 cc and,stability
R1740 T6014 T6011 conj translation,stability
R1741 T6015 T5895 punct .,contains
R1742 T6017 T6018 det The,transgene
R1743 T6018 T6024 nsubj transgene,purified
R1744 T6019 T6020 nummod 2.48,kb
R1745 T6020 T6018 compound kb,transgene
R1746 T6021 T6022 compound TRE,E2
R1747 T6022 T6018 compound E2,transgene
R1748 T6023 T6022 punct -,E2
R1749 T6025 T6024 aux was,purified
R1750 T6026 T6024 prep from,purified
R1751 T6027 T6028 compound vector,sequences
R1752 T6028 T6026 pobj sequences,from
R1753 T6029 T6024 prep following,purified
R1754 T6030 T6029 pobj digestion,following
R1755 T6031 T6030 prep with,digestion
R1756 T6032 T6031 pobj XhoI,with
R1757 T6033 T6032 cc and,XhoI
R1758 T6034 T6032 conj BamHI,XhoI
R1759 T6035 T6024 cc and,purified
R1760 T6036 T6024 conj injected,purified
R1761 T6037 T6036 prep into,injected
R1762 T6038 T6039 nmod C57BL,6J
R1763 T6039 T6041 nmod 6J,Strain
R1764 T6040 T6039 punct /,6J
R1765 T6041 T6047 compound Strain,embryos
R1766 T6042 T6039 cc or,6J
R1767 T6043 T6044 compound C57BL,6J
R1768 T6044 T6039 conj 6J,6J
R1769 T6045 T6044 punct /,6J
R1770 T6046 T6041 punct ×,Strain
R1771 T6047 T6037 pobj embryos,into
R1772 T6048 T6047 compound 129SvEv,embryos
R1773 T6049 T6047 compound mouse,embryos
R1774 T6050 T6024 prep at,purified
R1775 T6051 T6052 det the,facilities
R1776 T6052 T6050 pobj facilities,at
R1777 T6053 T6052 amod transgenic,facilities
R1778 T6054 T6052 compound core,facilities
R1779 T6055 T6052 prep of,facilities
R1780 T6056 T6057 det the,University
R1781 T6057 T6055 pobj University,of
R1782 T6058 T6057 prep of,University
R1783 T6059 T6058 pobj Pittsburgh,of
R1784 T6060 T6057 cc and,University
R1785 T6061 T6062 det the,University
R1786 T6062 T6057 conj University,University
R1787 T6063 T6062 prep of,University
R1788 T6064 T6063 pobj Cincinnati,of
R1789 T6065 T6024 punct ", ",purified
R1790 T6066 T6024 advmod respectively,purified
R1791 T6067 T6024 punct .,purified
R1792 T6069 T6070 amod Genomic,DNA
R1793 T6070 T6071 nsubjpass DNA,screened
R1794 T6072 T6070 prep from,DNA
R1795 T6073 T6074 det the,tail
R1796 T6074 T6072 pobj tail,from
R1797 T6075 T6074 prep of,tail
R1798 T6076 T6075 pobj mice,of
R1799 T6077 T6071 auxpass was,screened
R1800 T6078 T6071 prep by,screened
R1801 T6079 T6080 compound Southern,blot
R1802 T6080 T6081 compound blot,analysis
R1803 T6081 T6078 pobj analysis,by
R1804 T6082 T6071 prep following,screened
R1805 T6083 T6082 pobj digestion,following
R1806 T6084 T6083 prep with,digestion
R1807 T6085 T6084 pobj EcoRI,with
R1808 T6086 T6083 cc and,digestion
R1809 T6087 T6083 conj hybridization,digestion
R1810 T6088 T6087 prep to,hybridization
R1811 T6089 T6090 det an,probe
R1812 T6090 T6088 pobj probe,to
R1813 T6091 T6092 punct ~,400
R1814 T6092 T6093 nummod 400,bp
R1815 T6093 T6090 compound bp,probe
R1816 T6094 T6090 acl derived,probe
R1817 T6095 T6094 prep from,derived
R1818 T6096 T6097 det the,portion
R1819 T6097 T6095 pobj portion,from
R1820 T6098 T6097 compound SV40,portion
R1821 T6099 T6097 prep of,portion
R1822 T6100 T6101 det the,transgene
R1823 T6101 T6099 pobj transgene,of
R1824 T6102 T6103 compound TRE,E2
R1825 T6103 T6101 compound E2,transgene
R1826 T6104 T6103 punct -,E2
R1827 T6105 T6071 punct .,screened
R1828 T6427 T6426 prep of,Production
R1829 T6428 T6429 amod intermediate,model
R1830 T6429 T6427 pobj model,of
R1831 T6430 T6429 nmod MSUD,model
R1832 T6431 T6429 amod murine,model
R1833 T6433 T6434 det The,lines
R1834 T6434 T6440 nsubjpass lines,mated
R1835 T6435 T6434 amod various,lines
R1836 T6436 T6437 nmod TRE,E2
R1837 T6437 T6434 nmod E2,lines
R1838 T6438 T6437 punct -,E2
R1839 T6439 T6434 amod transgenic,lines
R1840 T6441 T6434 acl produced,lines
R1841 T6442 T6440 auxpass were,mated
R1842 T6443 T6440 advmod independently,mated
R1843 T6444 T6440 prep to,mated
R1844 T6445 T6444 pobj mice,to
R1845 T6446 T6447 dep that,were
R1846 T6447 T6445 relcl were,mice
R1847 T6448 T6447 acomp heterozygous,were
R1848 T6449 T6448 prep for,heterozygous
R1849 T6450 T6451 preconj both,knockout
R1850 T6451 T6449 pobj knockout,for
R1851 T6452 T6451 det the,knockout
R1852 T6453 T6451 compound E2,knockout
R1853 T6454 T6451 cc and,knockout
R1854 T6455 T6456 det the,transgene
R1855 T6456 T6451 conj transgene,knockout
R1856 T6457 T6458 compound LAP,tTA
R1857 T6458 T6456 compound tTA,transgene
R1858 T6459 T6458 punct -,tTA
R1859 T6460 T6461 punct [,background
R1860 T6461 T6440 parataxis background,mated
R1861 T6462 T6463 nmod Tg,Stock
R1862 T6463 T6461 dep Stock,background
R1863 T6464 T6465 punct (,tTALap
R1864 T6465 T6463 parataxis tTALap,Stock
R1865 T6466 T6465 punct ),tTALap
R1866 T6467 T6468 nmod Bjd,J
R1867 T6468 T6463 nmod J,Stock
R1868 T6469 T6468 punct /,J
R1869 T6470 T6463 punct ;,Stock
R1870 T6471 T6463 nummod 3272,Stock
R1871 T6472 T6461 punct ;,background
R1872 T6473 T6474 det The,Laboratory
R1873 T6474 T6461 dep Laboratory,background
R1874 T6475 T6474 compound Jackson,Laboratory
R1875 T6476 T6474 punct ", ",Laboratory
R1876 T6477 T6478 compound Bar,Harbor
R1877 T6478 T6474 npadvmod Harbor,Laboratory
R1878 T6479 T6474 punct ", ",Laboratory
R1879 T6480 T6474 npadvmod ME,Laboratory
R1880 T6481 T6461 punct ;,background
R1881 T6482 T6461 nmod NMRI,background
R1882 T6483 T6482 punct ×,NMRI
R1883 T6484 T6482 appos FVB,NMRI
R1884 T6485 T6482 punct ×,NMRI
R1885 T6486 T6487 compound C57BL,6J
R1886 T6487 T6482 appos 6J,NMRI
R1887 T6488 T6487 punct /,6J
R1888 T6489 T6461 punct ],background
R1889 T6490 T6440 punct .,mated
R1890 T6492 T6493 nsubj Interbreeding,resulted
R1891 T6494 T6492 prep of,Interbreeding
R1892 T6495 T6494 pobj animals,of
R1893 T6496 T6497 dep that,were
R1894 T6497 T6495 relcl were,animals
R1895 T6498 T6497 acomp heterozygous,were
R1896 T6499 T6498 prep for,heterozygous
R1897 T6500 T6501 det both,transgenes
R1898 T6501 T6499 pobj transgenes,for
R1899 T6502 T6501 cc and,transgenes
R1900 T6503 T6504 det the,knockout
R1901 T6504 T6501 conj knockout,transgenes
R1902 T6505 T6493 prep in,resulted
R1903 T6506 T6507 det the,production
R1904 T6507 T6505 pobj production,in
R1905 T6508 T6507 prep of,production
R1906 T6509 T6508 pobj mice,of
R1907 T6510 T6509 prep with,mice
R1908 T6511 T6512 det a,variety
R1909 T6512 T6510 pobj variety,with
R1910 T6513 T6512 prep of,variety
R1911 T6514 T6513 pobj genotypes,of
R1912 T6515 T6509 prep including,mice
R1913 T6516 T6517 det some,animals
R1914 T6517 T6515 pobj animals,including
R1915 T6518 T6519 dep that,were
R1916 T6519 T6517 relcl were,animals
R1917 T6520 T6519 acomp homozygous,were
R1918 T6521 T6520 prep for,homozygous
R1919 T6522 T6523 det the,knockout
R1920 T6523 T6521 pobj knockout,for
R1921 T6524 T6519 cc and,were
R1922 T6525 T6519 conj were,were
R1923 T6526 T6525 acomp positive,were
R1924 T6527 T6526 prep for,positive
R1925 T6528 T6529 det both,transgenes
R1926 T6529 T6527 pobj transgenes,for
R1927 T6530 T6531 punct (,refer
R1928 T6531 T6525 parataxis refer,were
R1929 T6532 T6531 nsubj we,refer
R1930 T6533 T6531 prep to,refer
R1931 T6534 T6535 det this,genotype
R1932 T6535 T6533 pobj genotype,to
R1933 T6536 T6531 prep as,refer
R1934 T6537 T6538 det the,genotype
R1935 T6538 T6536 pobj genotype,as
R1936 T6539 T6538 punct """",genotype
R1937 T6540 T6538 nmod rescue,genotype
R1938 T6541 T6538 punct """",genotype
R1939 T6542 T6531 punct ),refer
R1940 T6543 T6493 punct .,resulted
R1941 T6545 T6546 mark If,were
R1942 T6546 T6558 advcl were,survive
R1943 T6547 T6548 poss our,strategy
R1944 T6548 T6546 nsubj strategy,were
R1945 T6549 T6548 prep for,strategy
R1946 T6550 T6549 pcomp rescuing,for
R1947 T6551 T6552 det the,phenotype
R1948 T6552 T6550 dobj phenotype,rescuing
R1949 T6553 T6552 amod neonatal,phenotype
R1950 T6554 T6552 amod lethal,phenotype
R1951 T6555 T6552 prep of,phenotype
R1952 T6556 T6557 det the,knockout
R1953 T6557 T6555 pobj knockout,of
R1954 T6559 T6546 acomp successful,were
R1955 T6560 T6558 punct ", ",survive
R1956 T6561 T6558 advmod then,survive
R1957 T6562 T6563 det those,animals
R1958 T6563 T6558 nsubj animals,survive
R1959 T6564 T6563 amod homozygous,animals
R1960 T6565 T6563 compound knockout,animals
R1961 T6566 T6567 dep that,had
R1962 T6567 T6563 relcl had,animals
R1963 T6568 T6569 det both,transgenes
R1964 T6569 T6567 dobj transgenes,had
R1965 T6570 T6558 aux would,survive
R1966 T6571 T6558 prep beyond,survive
R1967 T6572 T6573 det the,period
R1968 T6573 T6571 pobj period,beyond
R1969 T6574 T6573 amod neonatal,period
R1970 T6575 T6558 punct .,survive
R1971 T6672 T6673 det All,mice
R1972 T6673 T6674 nsubjpass mice,genotyped
R1973 T6675 T6674 auxpass were,genotyped
R1974 T6676 T6674 prep by,genotyped
R1975 T6677 T6678 compound Southern,blot
R1976 T6678 T6679 compound blot,analysis
R1977 T6679 T6676 pobj analysis,by
R1978 T6680 T6674 punct .,genotyped
R1979 T6682 T6683 amod Genomic,DNAs
R1980 T6683 T6684 nsubjpass DNAs,digested
R1981 T6685 T6683 acl prepared,DNAs
R1982 T6686 T6685 prep from,prepared
R1983 T6687 T6688 compound tail,snips
R1984 T6688 T6686 pobj snips,from
R1985 T6689 T6684 auxpass were,digested
R1986 T6690 T6684 prep with,digested
R1987 T6691 T6692 det an,enzyme
R1988 T6692 T6690 pobj enzyme,with
R1989 T6693 T6692 amod appropriate,enzyme
R1990 T6694 T6692 compound restriction,enzyme
R1991 T6695 T6684 punct ", ",digested
R1992 T6696 T6697 dep size,fractionated
R1993 T6697 T6684 conj fractionated,digested
R1994 T6698 T6697 prep by,fractionated
R1995 T6699 T6700 compound agarose,gel
R1996 T6700 T6701 compound gel,electrophoresis
R1997 T6701 T6698 pobj electrophoresis,by
R1998 T6702 T6697 punct ", ",fractionated
R1999 T6703 T6697 conj blotted,fractionated
R2000 T6704 T6703 prep to,blotted
R2001 T6705 T6704 pobj nylon,to
R2002 T6706 T6703 punct ", ",blotted
R2003 T6707 T6703 cc and,blotted
R2004 T6708 T6703 conj probed,blotted
R2005 T6709 T6708 advcl using,probed
R2006 T6710 T6711 amod standard,procedures
R2007 T6711 T6709 dobj procedures,using
R2008 T6712 T6684 punct .,digested
R2009 T7185 T7186 amod Primary,fibroblasts
R2010 T7186 T7189 nsubjpass fibroblasts,prepared
R2011 T7187 T7186 nmod mouse,fibroblasts
R2012 T7188 T7186 amod embryonic,fibroblasts
R2013 T7190 T7189 auxpass were,prepared
R2014 T7191 T7189 prep from,prepared
R2015 T7192 T7193 amod embryonic,fetuses
R2016 T7193 T7191 pobj fetuses,from
R2017 T7194 T7195 nmod day,16.5
R2018 T7195 T7193 nummod 16.5,fetuses
R2019 T7196 T7195 punct ~,16.5
R2020 T7197 T7198 punct –,18.5
R2021 T7198 T7195 prep 18.5,16.5
R2022 T7199 T7200 mark as,described
R2023 T7200 T7189 advcl described,prepared
R2024 T7201 T7202 punct [,29
R2025 T7202 T7189 parataxis 29,prepared
R2026 T7203 T7202 punct ],29
R2027 T7204 T7189 punct .,prepared
R2028 T7206 T7207 nsubjpass Fibroblasts,passed
R2029 T7208 T7207 auxpass were,passed
R2030 T7209 T7207 prep onto,passed
R2031 T7210 T7209 pobj glass,onto
R2032 T7211 T7207 punct ", ",passed
R2033 T7212 T7207 conj fixed,passed
R2034 T7213 T7212 prep in,fixed
R2035 T7214 T7215 nummod 2,%
R2036 T7215 T7216 compound %,paraformaldehyde
R2037 T7216 T7213 pobj paraformaldehyde,in
R2038 T7217 T7212 prep in,fixed
R2039 T7218 T7217 pobj PBS,in
R2040 T7219 T7212 prep for,fixed
R2041 T7220 T7221 nummod 10,minutes
R2042 T7221 T7219 pobj minutes,for
R2043 T7222 T7212 punct ", ",fixed
R2044 T7223 T7212 conj permeabilized,fixed
R2045 T7224 T7223 prep in,permeabilized
R2046 T7225 T7226 nummod 2,%
R2047 T7226 T7227 compound %,paraformaldehyde
R2048 T7227 T7224 pobj paraformaldehyde,in
R2049 T7228 T7227 acl containing,paraformaldehyde
R2050 T7229 T7230 nummod 0.1,%
R2051 T7230 T7231 compound %,X100
R2052 T7231 T7228 dobj X100,containing
R2053 T7232 T7231 compound Triton,X100
R2054 T7233 T7223 prep for,permeabilized
R2055 T7234 T7235 nummod 10,minutes
R2056 T7235 T7233 pobj minutes,for
R2057 T7236 T7223 cc and,permeabilized
R2058 T7237 T7223 conj washed,permeabilized
R2059 T7238 T7239 nummod three,times
R2060 T7239 T7237 npadvmod times,washed
R2061 T7240 T7237 prep in,washed
R2062 T7241 T7240 pobj PBS,in
R2063 T7242 T7241 acl containing,PBS
R2064 T7243 T7244 nummod 0.5,%
R2065 T7244 T7245 compound %,BSA
R2066 T7245 T7242 dobj BSA,containing
R2067 T7246 T7245 cc and,BSA
R2068 T7247 T7248 nummod 0.15,%
R2069 T7248 T7249 compound %,glycine
R2070 T7249 T7245 conj glycine,BSA
R2071 T7250 T7242 punct ", ",containing
R2072 T7251 T7242 npadvmod pH,containing
R2073 T7252 T7251 nummod 7.4,pH
R2074 T7253 T7254 punct (,A
R2075 T7254 T7242 parataxis A,containing
R2076 T7255 T7254 compound Buffer,A
R2077 T7256 T7254 punct ),A
R2078 T7257 T7207 punct .,passed
R2079 T7259 T7260 prep Following,incubated
R2080 T7261 T7262 det a,incubation
R2081 T7262 T7259 pobj incubation,Following
R2082 T7263 T7264 nummod 30,min
R2083 T7264 T7262 compound min,incubation
R2084 T7265 T7262 prep with,incubation
R2085 T7266 T7267 amod purified,IgG
R2086 T7267 T7265 pobj IgG,with
R2087 T7268 T7267 compound goat,IgG
R2088 T7269 T7270 punct (,μg
R2089 T7270 T7267 parataxis μg,IgG
R2090 T7271 T7270 nummod 50,μg
R2091 T7272 T7270 punct (,μg
R2092 T7273 T7274 punct /,ml
R2093 T7274 T7270 prep ml,μg
R2094 T7275 T7270 punct ),μg
R2095 T7276 T7262 prep at,incubation
R2096 T7277 T7278 nummod 25,°C
R2097 T7278 T7276 pobj °C,at
R2098 T7279 T7262 cc and,incubation
R2099 T7280 T7281 nummod three,washes
R2100 T7281 T7262 conj washes,incubation
R2101 T7282 T7281 amod additional,washes
R2102 T7283 T7281 prep with,washes
R2103 T7284 T7285 compound Buffer,A
R2104 T7285 T7283 pobj A,with
R2105 T7286 T7260 punct ", ",incubated
R2106 T7287 T7260 nsubjpass cells,incubated
R2107 T7288 T7260 auxpass were,incubated
R2108 T7289 T7260 prep for,incubated
R2109 T7290 T7291 nummod 60,min
R2110 T7291 T7289 pobj min,for
R2111 T7292 T7260 prep with,incubated
R2112 T7293 T7294 npadvmod E2,specific
R2113 T7294 T7296 amod specific,antiserum
R2114 T7295 T7294 punct -,specific
R2115 T7296 T7292 pobj antiserum,with
R2116 T7297 T7298 punct [,30
R2117 T7298 T7296 parataxis 30,antiserum
R2118 T7299 T7298 punct ],30
R2119 T7300 T7296 prep at,antiserum
R2120 T7301 T7302 nummod 1,μg
R2121 T7302 T7300 pobj μg,at
R2122 T7303 T7304 punct /,ml
R2123 T7304 T7302 prep ml,μg
R2124 T7305 T7260 advcl followed,incubated
R2125 T7306 T7305 agent by,followed
R2126 T7307 T7308 nummod three,washes
R2127 T7308 T7306 pobj washes,by
R2128 T7309 T7308 prep in,washes
R2129 T7310 T7311 compound Buffer,A
R2130 T7311 T7309 pobj A,in
R2131 T7312 T7308 cc and,washes
R2132 T7313 T7314 nummod 60,minute
R2133 T7314 T7315 compound minute,incubation
R2134 T7315 T7308 conj incubation,washes
R2135 T7316 T7315 prep in,incubation
R2136 T7317 T7318 advmod fluorescently,labeled
R2137 T7318 T7319 amod labeled,antibody
R2138 T7319 T7316 pobj antibody,in
R2139 T7320 T7319 amod second,antibody
R2140 T7321 T7322 punct (,μg
R2141 T7322 T7260 parataxis μg,incubated
R2142 T7323 T7324 quantmod 1,2
R2143 T7324 T7322 nummod 2,μg
R2144 T7325 T7324 punct –,2
R2145 T7326 T7327 punct /,ml
R2146 T7327 T7322 prep ml,μg
R2147 T7328 T7322 punct ),μg
R2148 T7329 T7260 punct .,incubated
R2149 T7331 T7332 det The,cells
R2150 T7332 T7333 nsubjpass cells,washed
R2151 T7334 T7333 auxpass were,washed
R2152 T7335 T7333 advmod then,washed
R2153 T7336 T7337 nummod six,times
R2154 T7337 T7333 npadvmod times,washed
R2155 T7338 T7339 punct (,min
R2156 T7339 T7337 parataxis min,times
R2157 T7340 T7339 nummod 5,min
R2158 T7341 T7342 punct /,wash
R2159 T7342 T7339 prep wash,min
R2160 T7343 T7339 punct ),min
R2161 T7344 T7333 prep in,washed
R2162 T7345 T7346 compound Buffer,A
R2163 T7346 T7344 pobj A,in
R2164 T7347 T7333 cc and,washed
R2165 T7348 T7349 advmod then,mounted
R2166 T7349 T7333 conj mounted,washed
R2167 T7350 T7349 prep in,mounted
R2168 T7351 T7350 pobj gelvatol,in
R2169 T7352 T7353 punct (,Monsanto
R2170 T7353 T7351 parataxis Monsanto,gelvatol
R2171 T7354 T7353 punct ", ",Monsanto
R2172 T7355 T7356 compound St,Louis
R2173 T7356 T7353 npadvmod Louis,Monsanto
R2174 T7357 T7353 punct ),Monsanto
R2175 T7358 T7333 punct .,washed
R2176 T7360 T7361 advmod When,examined
R2177 T7361 T7367 advcl examined,was
R2178 T7362 T7361 nsubjpass livers,examined
R2179 T7363 T7362 prep from,livers
R2180 T7364 T7365 amod newborn,pups
R2181 T7365 T7363 pobj pups,from
R2182 T7366 T7361 auxpass were,examined
R2183 T7368 T7367 punct ", ",was
R2184 T7369 T7367 nsubj fixation,was
R2185 T7370 T7367 prep by,was
R2186 T7371 T7370 pobj immersion,by
R2187 T7372 T7371 prep in,immersion
R2188 T7373 T7374 nummod 2,%
R2189 T7374 T7375 compound %,paraformaldehyde
R2190 T7375 T7372 pobj paraformaldehyde,in
R2191 T7376 T7371 acl followed,immersion
R2192 T7377 T7376 agent by,followed
R2193 T7378 T7377 pobj cryoprotection,by
R2194 T7379 T7378 cc and,cryoprotection
R2195 T7380 T7381 compound shock,freezing
R2196 T7381 T7378 conj freezing,cryoprotection
R2197 T7382 T7378 prep in,cryoprotection
R2198 T7383 T7384 amod liquid,nitrogen
R2199 T7384 T7385 npadvmod nitrogen,cooled
R2200 T7385 T7386 amod cooled,isopentane
R2201 T7386 T7382 pobj isopentane,in
R2202 T7387 T7378 cc and,cryoprotection
R2203 T7388 T7378 conj sectioning,cryoprotection
R2204 T7389 T7390 punct (,microns
R2205 T7390 T7388 parataxis microns,sectioning
R2206 T7391 T7390 nummod 5,microns
R2207 T7392 T7390 punct ),microns
R2208 T7393 T7367 punct .,was
R2209 T7395 T7396 advmod Otherwise,was
R2210 T7397 T7396 nsubj processing,was
R2211 T7398 T7396 prep as,was
R2212 T7399 T7398 prep for,as
R2213 T7400 T7401 det the,cells
R2214 T7401 T7399 pobj cells,for
R2215 T7402 T7401 advmod above,cells
R2216 T7403 T7396 punct (,was
R2217 T7404 T7396 prep without,was
R2218 T7405 T7406 det the,steps
R2219 T7406 T7404 pobj steps,without
R2220 T7407 T7406 nmod fixation,steps
R2221 T7408 T7407 cc and,fixation
R2222 T7409 T7407 conj permabilization,fixation
R2223 T7410 T7396 punct ),was
R2224 T7411 T7396 punct .,was
R2225 T7545 T7546 compound Amino,acid
R2226 T7546 T7547 compound acid,analysis
R2227 T7549 T7550 nsubjpass Blood,collected
R2228 T7551 T7550 auxpass was,collected
R2229 T7552 T7550 prep from,collected
R2230 T7553 T7554 det the,vein
R2231 T7554 T7552 pobj vein,from
R2232 T7555 T7554 amod retroorbital,vein
R2233 T7556 T7554 nmod sinus,vein
R2234 T7557 T7556 cc or,sinus
R2235 T7558 T7556 conj tail,sinus
R2236 T7559 T7554 prep of,vein
R2237 T7560 T7559 pobj mice,of
R2238 T7561 T7550 cc and,collected
R2239 T7562 T7550 conj spotted,collected
R2240 T7563 T7562 prep on,spotted
R2241 T7564 T7565 det a,paper
R2242 T7565 T7563 pobj paper,on
R2243 T7566 T7565 compound filter,paper
R2244 T7567 T7568 advmod routinely,used
R2245 T7568 T7565 acl used,paper
R2246 T7569 T7568 prep for,used
R2247 T7570 T7571 compound blood,analysis
R2248 T7571 T7569 pobj analysis,for
R2249 T7572 T7573 compound amino,acid
R2250 T7573 T7571 compound acid,analysis
R2251 T7574 T7571 prep for,analysis
R2252 T7575 T7576 amod prenatal,screening
R2253 T7576 T7574 pobj screening,for
R2254 T7577 T7550 punct .,collected
R2255 T7579 T7580 nsubjpass Concentrations,determined
R2256 T7581 T7579 prep of,Concentrations
R2257 T7582 T7581 pobj BCAA,of
R2258 T7583 T7582 cc and,BCAA
R2259 T7584 T7585 amod other,acids
R2260 T7585 T7582 conj acids,BCAA
R2261 T7586 T7585 compound amino,acids
R2262 T7587 T7579 prep in,Concentrations
R2263 T7588 T7587 pobj blood,in
R2264 T7589 T7580 auxpass were,determined
R2265 T7590 T7580 prep by,determined
R2266 T7591 T7592 amod tandem,spectrometry
R2267 T7592 T7590 pobj spectrometry,by
R2268 T7593 T7592 compound mass,spectrometry
R2269 T7594 T7595 punct (,Screening
R2270 T7595 T7592 parataxis Screening,spectrometry
R2271 T7596 T7595 compound Pediatrix,Screening
R2272 T7597 T7595 punct ", ",Screening
R2273 T7598 T7595 npadvmod Bridgeville,Screening
R2274 T7599 T7595 punct ", ",Screening
R2275 T7600 T7595 npadvmod PA,Screening
R2276 T7601 T7595 punct ),Screening
R2277 T7602 T7580 punct .,determined
R2282 T8106 T8105 prep of,Assay
R2283 T8107 T8108 compound BCKDH,activity
R2284 T8108 T8106 pobj activity,of
R2285 T8110 T8111 nsubjpass Livers,removed
R2286 T8112 T8111 auxpass were,removed
R2287 T8113 T8111 punct ", ",removed
R2288 T8114 T8111 conj frozen,removed
R2289 T8115 T8114 prep in,frozen
R2290 T8116 T8117 amod liquid,nitrogen
R2291 T8117 T8115 pobj nitrogen,in
R2292 T8118 T8114 punct ", ",frozen
R2293 T8119 T8114 cc and,frozen
R2294 T8120 T8114 conj stored,frozen
R2295 T8121 T8120 prep at,stored
R2296 T8122 T8123 punct -,80
R2297 T8123 T8124 nummod 80,°C
R2298 T8124 T8121 pobj °C,at
R2299 T8125 T8111 punct .,removed
R2300 T8127 T8128 prep At,thawed
R2301 T8129 T8130 det the,time
R2302 T8130 T8127 pobj time,At
R2303 T8131 T8130 prep of,time
R2304 T8132 T8133 compound enzyme,assay
R2305 T8133 T8131 pobj assay,of
R2306 T8134 T8128 punct ", ",thawed
R2307 T8135 T8128 nsubjpass livers,thawed
R2308 T8136 T8128 auxpass were,thawed
R2309 T8137 T8128 punct ", ",thawed
R2310 T8138 T8128 cc and,thawed
R2311 T8139 T8128 conj homogenized,thawed
R2312 T8140 T8141 punct (,v
R2313 T8141 T8139 parataxis v,homogenized
R2314 T8142 T8143 nummod 1,9
R2315 T8143 T8141 dep 9,v
R2316 T8144 T8143 punct :,9
R2317 T8145 T8141 punct ", ",v
R2318 T8146 T8141 compound w,v
R2319 T8147 T8141 punct /,v
R2320 T8148 T8141 punct ),v
R2321 T8149 T8139 prep in,homogenized
R2322 T8150 T8151 nummod 0.25,M
R2323 T8151 T8152 compound M,sucrose
R2324 T8152 T8149 pobj sucrose,in
R2325 T8153 T8152 punct ", ",sucrose
R2326 T8154 T8155 nummod 10,mM
R2327 T8155 T8156 compound mM,HC1
R2328 T8156 T8152 appos HC1,sucrose
R2329 T8157 T8156 compound Tris,HC1
R2330 T8158 T8156 punct -,HC1
R2331 T8159 T8156 punct ", ",HC1
R2332 T8160 T8156 npadvmod pH,HC1
R2333 T8161 T8160 nummod 7.4,pH
R2334 T8162 T8128 punct .,thawed
R2335 T8164 T8165 compound Liver,homogenates
R2336 T8165 T8166 nsubjpass homogenates,centrifuged
R2337 T8167 T8166 auxpass were,centrifuged
R2338 T8168 T8166 prep at,centrifuged
R2339 T8169 T8170 nummod 600,g
R2340 T8170 T8168 pobj g,at
R2341 T8171 T8169 punct ×,600
R2342 T8172 T8166 prep for,centrifuged
R2343 T8173 T8174 nummod 10,min
R2344 T8174 T8172 pobj min,for
R2345 T8175 T8166 prep at,centrifuged
R2346 T8176 T8177 nummod 4,°C
R2347 T8177 T8175 pobj °C,at
R2348 T8178 T8166 cc and,centrifuged
R2349 T8179 T8180 det the,fraction
R2350 T8180 T8182 nsubjpass fraction,saved
R2351 T8181 T8180 compound supernatant,fraction
R2352 T8182 T8166 conj saved,centrifuged
R2353 T8183 T8182 auxpass was,saved
R2354 T8184 T8185 aux to,determine
R2355 T8185 T8182 advcl determine,saved
R2356 T8186 T8187 det the,activity
R2357 T8187 T8185 dobj activity,determine
R2358 T8188 T8187 compound BCKDH,activity
R2359 T8189 T8182 punct .,saved
R2360 T8191 T8192 det The,use
R2361 T8192 T8193 nsubjpass use,necessitated
R2362 T8194 T8192 prep of,use
R2363 T8195 T8196 compound tissue,homogenates
R2364 T8196 T8194 pobj homogenates,of
R2365 T8197 T8193 auxpass was,necessitated
R2366 T8198 T8193 agent by,necessitated
R2367 T8199 T8200 det the,availability
R2368 T8200 T8198 pobj availability,by
R2369 T8201 T8200 amod limited,availability
R2370 T8202 T8200 prep of,availability
R2371 T8203 T8204 compound liver,tissue
R2372 T8204 T8202 pobj tissue,of
R2373 T8205 T8204 punct ", ",tissue
R2374 T8206 T8207 advmod particularly,from
R2375 T8207 T8204 prep from,tissue
R2376 T8208 T8209 amod newborn,pups
R2377 T8209 T8207 pobj pups,from
R2378 T8210 T8193 punct .,necessitated
R2379 T8212 T8213 compound BCKDH,activity
R2380 T8213 T8214 nsubjpass activity,determined
R2381 T8215 T8214 auxpass was,determined
R2382 T8216 T8214 prep by,determined
R2383 T8217 T8216 pcomp measuring,by
R2384 T8218 T8219 det the,release
R2385 T8219 T8217 dobj release,measuring
R2386 T8220 T8219 prep of,release
R2387 T8221 T8220 pobj 14CO2,of
R2388 T8222 T8217 prep from,measuring
R2389 T8223 T8224 nmod α,keto
R2390 T8224 T8226 nmod keto,isocaproate
R2391 T8225 T8224 punct -,keto
R2392 T8226 T8222 pobj isocaproate,from
R2393 T8227 T8228 punct [,1
R2394 T8228 T8224 parataxis 1,keto
R2395 T8229 T8230 punct -,14C
R2396 T8230 T8228 prep 14C,1
R2397 T8231 T8228 punct ],1
R2398 T8232 T8233 mark as,described
R2399 T8233 T8214 advcl described,determined
R2400 T8234 T8233 advmod previously,described
R2401 T8235 T8236 punct [,31
R2402 T8236 T8233 parataxis 31,described
R2403 T8237 T8236 punct ],31
R2404 T8238 T8214 punct .,determined
R2405 T8240 T8241 det The,mixture
R2406 T8241 T8244 nsubj mixture,contained
R2407 T8242 T8241 amod complete,mixture
R2408 T8243 T8241 compound reaction,mixture
R2409 T8245 T8246 punct (,volume
R2410 T8246 T8244 parataxis volume,contained
R2411 T8247 T8246 amod final,volume
R2412 T8248 T8249 nummod 1,ml
R2413 T8249 T8246 appos ml,volume
R2414 T8250 T8246 punct ),volume
R2415 T8251 T8252 nummod 30,mM
R2416 T8252 T8253 compound mM,buffer
R2417 T8253 T8244 dobj buffer,contained
R2418 T8254 T8255 compound potassium,phosphate
R2419 T8255 T8253 compound phosphate,buffer
R2420 T8256 T8253 punct ", ",buffer
R2421 T8257 T8253 npadvmod pH,buffer
R2422 T8258 T8257 nummod 7.4,pH
R2423 T8259 T8253 punct ", ",buffer
R2424 T8260 T8261 nummod 0.20,mM
R2425 T8261 T8262 compound mM,ketoisocaproate
R2426 T8262 T8253 conj ketoisocaproate,buffer
R2427 T8263 T8262 compound α,ketoisocaproate
R2428 T8264 T8262 punct -,ketoisocaproate
R2429 T8265 T8262 punct ", ",ketoisocaproate
R2430 T8266 T8267 nummod 0.40,mM
R2431 T8267 T8268 compound mM,CoASH
R2432 T8268 T8262 conj CoASH,ketoisocaproate
R2433 T8269 T8268 punct ", ",CoASH
R2434 T8270 T8271 nummod 0.40,mM
R2435 T8271 T8272 compound mM,pyrophosphate
R2436 T8272 T8268 conj pyrophosphate,CoASH
R2437 T8273 T8272 compound thiamin,pyrophosphate
R2438 T8274 T8272 punct ", ",pyrophosphate
R2439 T8275 T8276 nummod 2,mM
R2440 T8276 T8277 compound mM,NAD
R2441 T8277 T8272 conj NAD,pyrophosphate
R2442 T8278 T8277 punct +,NAD
R2443 T8279 T8277 punct ", ",NAD
R2444 T8280 T8281 nummod 2,mM
R2445 T8281 T8282 compound mM,dithiothreitol
R2446 T8282 T8277 conj dithiothreitol,NAD
R2447 T8283 T8282 punct ", ",dithiothreitol
R2448 T8284 T8285 nummod 5,mM
R2449 T8285 T8286 compound mM,Mg2
R2450 T8286 T8282 conj Mg2,dithiothreitol
R2451 T8287 T8286 punct +,Mg2
R2452 T8288 T8286 punct ", ",Mg2
R2453 T8289 T8290 advmod approximately,"250,000"
R2454 T8290 T8291 nummod "250,000",DPM
R2455 T8291 T8286 conj DPM,Mg2
R2456 T8292 T8291 prep of,DPM
R2457 T8293 T8294 nmod α,keto
R2458 T8294 T8296 nmod keto,isocaproate
R2459 T8295 T8294 punct -,keto
R2460 T8296 T8292 pobj isocaproate,of
R2461 T8297 T8298 punct [,1
R2462 T8298 T8294 parataxis 1,keto
R2463 T8299 T8300 punct -,14C
R2464 T8300 T8298 prep 14C,1
R2465 T8301 T8298 punct ],1
R2466 T8302 T8291 punct ", ",DPM
R2467 T8303 T8291 cc and,DPM
R2468 T8304 T8305 nummod 0.10,ml
R2469 T8305 T8291 conj ml,DPM
R2470 T8306 T8305 prep of,ml
R2471 T8307 T8308 compound liver,homogenate
R2472 T8308 T8306 pobj homogenate,of
R2473 T8309 T8310 punct (,protein
R2474 T8310 T8308 parataxis protein,homogenate
R2475 T8311 T8312 quantmod 2,3
R2476 T8312 T8314 nummod 3,mg
R2477 T8313 T8312 punct –,3
R2478 T8314 T8310 compound mg,protein
R2479 T8315 T8310 punct ),protein
R2480 T8316 T8244 punct .,contained
R2481 T8318 T8319 nsubjpass Assays,carried
R2482 T8320 T8319 auxpass were,carried
R2483 T8321 T8319 prt out,carried
R2484 T8322 T8319 prep for,carried
R2485 T8323 T8324 nummod 15,min
R2486 T8324 T8322 pobj min,for
R2487 T8325 T8319 prep at,carried
R2488 T8326 T8327 nummod 37,°C
R2489 T8327 T8325 pobj °C,at
R2490 T8328 T8319 punct ", ",carried
R2491 T8329 T8330 nsubjpass 14CO2,trapped
R2492 T8330 T8319 conj trapped,carried
R2493 T8331 T8330 auxpass was,trapped
R2494 T8332 T8330 prep in,trapped
R2495 T8333 T8332 pobj hydroxide,in
R2496 T8334 T8333 prep of,hydroxide
R2497 T8335 T8334 pobj Hyamine,of
R2498 T8336 T8330 punct ", ",trapped
R2499 T8337 T8330 cc and,trapped
R2500 T8338 T8339 nsubjpass radioactivity,determined
R2501 T8339 T8330 conj determined,trapped
R2502 T8340 T8339 auxpass was,determined
R2503 T8341 T8339 prep by,determined
R2504 T8342 T8343 amod liquid,scintillation
R2505 T8343 T8344 compound scintillation,spectrometry
R2506 T8344 T8341 pobj spectrometry,by
R2507 T8345 T8339 punct .,determined
R2508 T8817 T8818 compound Western,blot
R2509 T8818 T8819 compound blot,analysis
R2510 T8821 T8822 compound Protein,extracts
R2511 T8822 T8823 nsubjpass extracts,isolated
R2512 T8824 T8823 auxpass were,isolated
R2513 T8825 T8823 prep from,isolated
R2514 T8826 T8827 amod homogenized,liver
R2515 T8827 T8825 pobj liver,from
R2516 T8828 T8827 punct (,liver
R2517 T8829 T8830 advmod freshly,harvested
R2518 T8830 T8827 acl harvested,liver
R2519 T8831 T8830 cc and,harvested
R2520 T8832 T8833 dep flash,frozen
R2521 T8833 T8830 conj frozen,harvested
R2522 T8834 T8827 punct ),liver
R2523 T8835 T8827 prep of,liver
R2524 T8836 T8837 npadvmod wildtype,transgenic
R2525 T8837 T8845 amod transgenic,mice
R2526 T8838 T8836 punct ", ",wildtype
R2527 T8839 T8840 compound Line,525A
R2528 T8840 T8836 conj 525A,wildtype
R2529 T8841 T8840 punct ", ",525A
R2530 T8842 T8840 cc and,525A
R2531 T8843 T8844 compound Line,A
R2532 T8844 T8840 conj A,525A
R2533 T8845 T8835 pobj mice,of
R2534 T8846 T8823 punct .,isolated
R2535 T8848 T8849 nsubjpass Protein,analyzed
R2536 T8850 T8851 punct (,μg
R2537 T8851 T8848 parataxis μg,Protein
R2538 T8852 T8851 nummod 25,μg
R2539 T8853 T8851 prep per,μg
R2540 T8854 T8853 pobj sample,per
R2541 T8855 T8851 punct ),μg
R2542 T8856 T8849 auxpass was,analyzed
R2543 T8857 T8849 prep by,analyzed
R2544 T8858 T8857 pobj electrophoresis,by
R2545 T8859 T8849 prep on,analyzed
R2546 T8860 T8861 det a,Gel
R2547 T8861 T8859 pobj Gel,on
R2548 T8862 T8863 nummod 10,%
R2549 T8863 T8861 nmod %,Gel
R2550 T8864 T8865 nmod SDS,PAGE
R2551 T8865 T8861 nmod PAGE,Gel
R2552 T8866 T8865 punct -,PAGE
R2553 T8867 T8861 amod Ready,Gel
R2554 T8868 T8869 punct (,Rad
R2555 T8869 T8861 parataxis Rad,Gel
R2556 T8870 T8869 compound Bio,Rad
R2557 T8871 T8869 punct -,Rad
R2558 T8872 T8869 punct ", ",Rad
R2559 T8873 T8869 npadvmod Hercules,Rad
R2560 T8874 T8869 punct ", ",Rad
R2561 T8875 T8869 npadvmod CA,Rad
R2562 T8876 T8869 punct ),Rad
R2563 T8877 T8849 cc and,analyzed
R2564 T8878 T8849 conj transferred,analyzed
R2565 T8879 T8878 prep to,transferred
R2566 T8880 T8881 compound PVDF,membrane
R2567 T8881 T8879 pobj membrane,to
R2568 T8882 T8883 punct (,Rad
R2569 T8883 T8881 parataxis Rad,membrane
R2570 T8884 T8885 compound Sequi,Blot
R2571 T8885 T8883 dep Blot,Rad
R2572 T8886 T8885 punct -,Blot
R2573 T8887 T8883 punct ;,Rad
R2574 T8888 T8883 compound Bio,Rad
R2575 T8889 T8883 punct -,Rad
R2576 T8890 T8883 punct ),Rad
R2577 T8891 T8878 prep via,transferred
R2578 T8892 T8891 pobj electroblotting,via
R2579 T8893 T8849 punct .,analyzed
R2580 T8895 T8896 det All,blots
R2581 T8896 T8897 nsubjpass blots,probed
R2582 T8898 T8897 auxpass were,probed
R2583 T8899 T8897 prep for,probed
R2584 T8900 T8901 compound E2,protein
R2585 T8901 T8899 pobj protein,for
R2586 T8902 T8897 advcl using,probed
R2587 T8903 T8904 amod polyclonal,antisera
R2588 T8904 T8902 dobj antisera,using
R2589 T8905 T8904 compound rabbit,antisera
R2590 T8906 T8904 compound E2,antisera
R2591 T8907 T8908 punct (,"5,000"
R2592 T8908 T8904 parataxis "5,000",antisera
R2593 T8909 T8908 nummod 1,"5,000"
R2594 T8910 T8908 punct :,"5,000"
R2595 T8911 T8908 punct ),"5,000"
R2596 T8912 T8904 punct ", ",antisera
R2597 T8913 T8914 dep which,detects
R2598 T8914 T8904 relcl detects,antisera
R2599 T8915 T8916 preconj both,subunits
R2600 T8916 T8914 dobj subunits,detects
R2601 T8917 T8916 nmod mouse,subunits
R2602 T8918 T8919 punct (,kD
R2603 T8919 T8917 parataxis kD,mouse
R2604 T8920 T8921 punct ~,47
R2605 T8921 T8919 nummod 47,kD
R2606 T8922 T8919 punct ),kD
R2607 T8923 T8917 cc and,mouse
R2608 T8924 T8917 conj human,mouse
R2609 T8925 T8926 punct (,kD
R2610 T8926 T8924 parataxis kD,human
R2611 T8927 T8928 punct ~,54
R2612 T8928 T8926 nummod 54,kD
R2613 T8929 T8926 punct ),kD
R2614 T8930 T8916 compound E2,subunits
R2615 T8931 T8932 punct (,gift
R2616 T8932 T8916 parataxis gift,subunits
R2617 T8933 T8932 punct [,gift
R2618 T8934 T8932 dep 30,gift
R2619 T8935 T8932 punct ],gift
R2620 T8936 T8932 punct ;,gift
R2621 T8937 T8932 prep from,gift
R2622 T8938 T8939 compound Dr.,Hutson
R2623 T8939 T8937 pobj Hutson,from
R2624 T8940 T8939 compound Susan,Hutson
R2625 T8941 T8939 punct ", ",Hutson
R2626 T8942 T8943 compound Wake,Forest
R2627 T8943 T8944 compound Forest,University
R2628 T8944 T8939 npadvmod University,Hutson
R2629 T8945 T8932 punct ),gift
R2630 T8946 T8897 punct .,probed
R2631 T8948 T8949 nsubjpass Blots,re-probed
R2632 T8950 T8949 auxpass were,re-probed
R2633 T8951 T8949 prep with,re-probed
R2634 T8952 T8953 det a,antibody
R2635 T8953 T8951 pobj antibody,with
R2636 T8954 T8953 nmod rabbit,antibody
R2637 T8955 T8956 amod anti-c,myc
R2638 T8956 T8953 compound myc,antibody
R2639 T8957 T8956 punct -,myc
R2640 T8958 T8953 compound tag,antibody
R2641 T8959 T8960 punct (,ab9106
R2642 T8960 T8953 parataxis ab9106,antibody
R2643 T8961 T8962 nummod 1,"10,000"
R2644 T8962 T8960 dep "10,000",ab9106
R2645 T8963 T8962 punct :,"10,000"
R2646 T8964 T8960 punct ;,ab9106
R2647 T8965 T8960 dep abCam,ab9106
R2648 T8966 T8965 punct ", ",abCam
R2649 T8967 T8965 npadvmod Cambridge,abCam
R2650 T8968 T8965 punct ", ",abCam
R2651 T8969 T8965 npadvmod MA,abCam
R2652 T8970 T8960 punct ;,ab9106
R2653 T8971 T8960 nmod cat.,ab9106
R2654 T8972 T8960 punct #,ab9106
R2655 T8973 T8960 punct -,ab9106
R2656 T8974 T8960 nummod 100,ab9106
R2657 T8975 T8960 punct ),ab9106
R2658 T8976 T8949 punct .,re-probed
R2659 T8978 T8979 nsubjpass Blots,re-probed
R2660 T8980 T8979 auxpass were,re-probed
R2661 T8981 T8979 advmod also,re-probed
R2662 T8982 T8979 prep with,re-probed
R2663 T8983 T8984 det an,antibody
R2664 T8984 T8982 pobj antibody,with
R2665 T8985 T8984 prep for,antibody
R2666 T8986 T8987 compound β,actin
R2667 T8987 T8985 pobj actin,for
R2668 T8988 T8987 punct -,actin
R2669 T8989 T8990 punct (,ab8227
R2670 T8990 T8987 parataxis ab8227,actin
R2671 T8991 T8992 nummod 43,kD
R2672 T8992 T8990 dep kD,ab8227
R2673 T8993 T8990 punct ;,ab8227
R2674 T8994 T8995 nummod 1,"10,000"
R2675 T8995 T8990 dep "10,000",ab8227
R2676 T8996 T8995 punct :,"10,000"
R2677 T8997 T8990 punct ;,ab8227
R2678 T8998 T8990 dep abCam,ab8227
R2679 T8999 T8990 punct ;,ab8227
R2680 T9000 T8990 nmod cat.,ab8227
R2681 T9001 T8990 punct #,ab8227
R2682 T9002 T8990 punct -,ab8227
R2683 T9003 T8990 nummod 50,ab8227
R2684 T9004 T8990 punct ),ab8227
R2685 T9005 T9006 aux to,allow
R2686 T9006 T8979 advcl allow,re-probed
R2687 T9007 T9006 prep for,allow
R2688 T9008 T9009 compound loading,comparisons
R2689 T9009 T9007 pobj comparisons,for
R2690 T9010 T8979 punct .,re-probed
R2691 T9012 T9013 det A,antibody
R2692 T9013 T9017 nsubjpass antibody,used
R2693 T9014 T9013 nmod goat,antibody
R2694 T9015 T9013 amod anti-rabbit,antibody
R2695 T9016 T9013 amod secondary,antibody
R2696 T9018 T9013 acl conjugated,antibody
R2697 T9019 T9018 prep to,conjugated
R2698 T9020 T9021 compound horseradish,peroxidase
R2699 T9021 T9019 pobj peroxidase,to
R2700 T9022 T9023 punct (,H
R2701 T9023 T9021 parataxis H,peroxidase
R2702 T9024 T9025 nummod 1,"10,000"
R2703 T9025 T9023 dep "10,000",H
R2704 T9026 T9025 punct :,"10,000"
R2705 T9027 T9023 punct ;,H
R2706 T9028 T9023 dep Novus,H
R2707 T9029 T9028 punct ", ",Novus
R2708 T9030 T9028 npadvmod Littleton,Novus
R2709 T9031 T9028 punct ", ",Novus
R2710 T9032 T9028 npadvmod CO,Novus
R2711 T9033 T9023 punct ;,H
R2712 T9034 T9023 nmod cat.,H
R2713 T9035 T9023 punct #,H
R2714 T9036 T9023 compound NB730,H
R2715 T9037 T9023 punct -,H
R2716 T9038 T9023 punct ),H
R2717 T9039 T9017 auxpass was,used
R2718 T9040 T9017 prep for,used
R2719 T9041 T9040 pobj detection,for
R2720 T9042 T9017 advcl using,used
R2721 T9043 T9044 det the,reagent
R2722 T9044 T9042 dobj reagent,using
R2723 T9045 T9046 compound Western,Lightning
R2724 T9046 T9044 compound Lightning,reagent
R2725 T9047 T9044 compound chemiluminescence,reagent
R2726 T9048 T9049 punct (,Elmer
R2727 T9049 T9044 parataxis Elmer,reagent
R2728 T9050 T9049 compound Perkin,Elmer
R2729 T9051 T9049 punct ", ",Elmer
R2730 T9052 T9049 npadvmod Boston,Elmer
R2731 T9053 T9049 punct ", ",Elmer
R2732 T9054 T9049 npadvmod MA,Elmer
R2733 T9055 T9049 punct ),Elmer
R2734 T9056 T9017 cc and,used
R2735 T9057 T9017 conj exposed,used
R2736 T9058 T9057 prep to,exposed
R2737 T9059 T9060 compound X-ray,film
R2738 T9060 T9058 pobj film,to
R2739 T9061 T9017 punct .,used
R2740 T9141 T9142 amod Statistical,analyses
R2741 T9144 T9145 det All,data
R2742 T9145 T9151 nsubjpass data,presented
R2743 T9146 T9145 nmod BCAA,data
R2744 T9147 T9146 cc and,BCAA
R2745 T9148 T9146 conj BCKDH,BCAA
R2746 T9149 T9145 compound enzyme,data
R2747 T9150 T9145 compound activity,data
R2748 T9152 T9151 auxpass are,presented
R2749 T9153 T9151 prep as,presented
R2750 T9154 T9155 det the,mean
R2751 T9155 T9153 pobj mean,as
R2752 T9156 T9155 cc +,mean
R2753 T9157 T9156 punct /,+
R2754 T9158 T9156 punct -,+
R2755 T9159 T9160 det the,error
R2756 T9160 T9155 conj error,mean
R2757 T9161 T9160 amod standard,error
R2758 T9162 T9160 prep of,error
R2759 T9163 T9164 det the,mean
R2760 T9164 T9162 pobj mean,of
R2761 T9165 T9160 punct (,error
R2762 T9166 T9160 appos S.E.M.,error
R2763 T9167 T9151 punct ),presented
R2764 T9168 T9151 punct .,presented
R2765 T9170 T9171 nsubjpass Differences,compared
R2766 T9172 T9170 prep between,Differences
R2767 T9173 T9172 pobj genotypes,between
R2768 T9174 T9171 auxpass were,compared
R2769 T9175 T9171 prep by,compared
R2770 T9176 T9177 poss Student,test
R2771 T9177 T9175 pobj test,by
R2772 T9178 T9176 case 's,Student
R2773 T9179 T9177 compound t,test
R2774 T9180 T9181 punct [,32
R2775 T9181 T9171 parataxis 32,compared
R2776 T9182 T9181 punct ],32
R2777 T9183 T9171 punct .,compared
R6272 T22055 T22056 compound E2,production
R6273 T22057 T22058 compound gene,knockout
R6274 T22058 T22056 compound knockout,production
R6275 T22059 T22056 compound mouse,production
R6276 T22060 T22056 punct .,production
R6277 T22062 T22063 meta A,strategy
R6278 T22064 T22063 punct ", ",strategy
R6279 T22065 T22066 compound Gene,targeting
R6280 T22066 T22063 compound targeting,strategy
R6281 T22067 T22063 acl used,strategy
R6282 T22068 T22067 prep for,used
R6283 T22069 T22068 pcomp targeting,for
R6284 T22070 T22071 det the,locus
R6285 T22071 T22069 dobj locus,targeting
R6286 T22072 T22071 compound E2,locus
R6287 T22073 T22067 prep in,used
R6288 T22074 T22075 compound mouse,cells
R6289 T22075 T22073 pobj cells,in
R6290 T22076 T22075 compound ES,cells
R6291 T22077 T22063 punct .,strategy
R6292 T22079 T22080 det The,construct
R6293 T22080 T22082 nsubjpass construct,designed
R6294 T22081 T22080 compound targeting,construct
R6295 T22083 T22082 auxpass was,designed
R6296 T22084 T22085 aux to,delete
R6297 T22085 T22082 advcl delete,designed
R6298 T22086 T22087 nummod 1.67,kb
R6299 T22087 T22085 dobj kb,delete
R6300 T22088 T22087 prep of,kb
R6301 T22089 T22088 pobj sequence,of
R6302 T22090 T22085 prep between,delete
R6303 T22091 T22092 det an,site
R6304 T22092 T22090 pobj site,between
R6305 T22093 T22092 compound EcoRV,site
R6306 T22094 T22092 prep in,site
R6307 T22095 T22094 pobj Exon,in
R6308 T22096 T22095 nummod 4,Exon
R6309 T22097 T22092 cc and,site
R6310 T22098 T22099 det a,site
R6311 T22099 T22092 conj site,site
R6312 T22100 T22099 compound Smal,site
R6313 T22101 T22099 prep in,site
R6314 T22102 T22101 pobj intron,in
R6315 T22103 T22102 nummod 5,intron
R6316 T22104 T22082 punct .,designed
R6317 T22106 T22107 det The,gene
R6318 T22107 T22111 nsubj gene,contains
R6319 T22108 T22109 amod wild,type
R6320 T22109 T22107 compound type,gene
R6321 T22110 T22107 compound E2,gene
R6322 T22112 T22113 det an,fragment
R6323 T22113 T22111 dobj fragment,contains
R6324 T22114 T22115 punct ~,16
R6325 T22115 T22116 nummod 16,kb
R6326 T22116 T22113 compound kb,fragment
R6327 T22117 T22113 compound BglI,fragment
R6328 T22118 T22113 compound restriction,fragment
R6329 T22119 T22120 dep that,hybridizes
R6330 T22120 T22113 relcl hybridizes,fragment
R6331 T22121 T22120 prep to,hybridizes
R6332 T22122 T22123 det the,probe
R6333 T22123 T22121 pobj probe,to
R6334 T22124 T22125 npadvmod Exon,specific
R6335 T22125 T22123 amod specific,probe
R6336 T22126 T22124 nummod 6,Exon
R6337 T22127 T22111 punct .,contains
R6338 T22129 T22130 det A,locus
R6339 T22130 T22134 nsubj locus,harbors
R6340 T22131 T22132 advmod correctly,targeted
R6341 T22132 T22130 amod targeted,locus
R6342 T22133 T22130 compound E2,locus
R6343 T22135 T22136 det an,fragment
R6344 T22136 T22134 dobj fragment,harbors
R6345 T22137 T22138 punct ~,11
R6346 T22138 T22139 nummod 11,kb
R6347 T22139 T22136 compound kb,fragment
R6348 T22140 T22136 compound BglI,fragment
R6349 T22141 T22136 compound restriction,fragment
R6350 T22142 T22143 dep that,hybridizes
R6351 T22143 T22136 relcl hybridizes,fragment
R6352 T22144 T22143 prep to,hybridizes
R6353 T22145 T22146 det the,probe
R6354 T22146 T22144 pobj probe,to
R6355 T22147 T22146 amod same,probe
R6356 T22148 T22134 punct .,harbors
R6357 T22151 T22152 mark that,detect
R6358 T22152 T22150 ccomp detect,Note
R6359 T22153 T22154 det the,probe
R6360 T22154 T22152 nsubj probe,detect
R6361 T22155 T22152 aux will,detect
R6362 T22156 T22152 neg not,detect
R6363 T22157 T22158 amod random,integration
R6364 T22158 T22152 dobj integration,detect
R6365 T22159 T22158 prep of,integration
R6366 T22160 T22161 det the,vector
R6367 T22161 T22159 pobj vector,of
R6368 T22162 T22161 compound targeting,vector
R6369 T22163 T22164 mark because,is
R6370 T22164 T22152 advcl is,detect
R6371 T22165 T22164 nsubj it,is
R6372 T22166 T22164 acomp external,is
R6373 T22167 T22166 prep to,external
R6374 T22168 T22169 det the,vector
R6375 T22169 T22167 pobj vector,to
R6376 T22170 T22169 compound targeting,vector
R6377 T22171 T22150 punct .,Note
R6378 T22173 T22174 meta B,analysis
R6379 T22175 T22174 punct ", ",analysis
R6380 T22176 T22177 compound Southern,blot
R6381 T22177 T22174 compound blot,analysis
R6382 T22178 T22174 prep of,analysis
R6383 T22179 T22180 npadvmod Bgll,digested
R6384 T22180 T22181 amod digested,DNA
R6385 T22181 T22178 pobj DNA,of
R6386 T22182 T22181 amod genomic,DNA
R6387 T22183 T22181 acl derived,DNA
R6388 T22184 T22183 prep from,derived
R6389 T22185 T22186 det the,line
R6390 T22186 T22184 pobj line,from
R6391 T22187 T22186 amod parental,line
R6392 T22188 T22189 amod wild,type
R6393 T22189 T22186 compound type,line
R6394 T22190 T22191 compound ES,cell
R6395 T22191 T22186 compound cell,line
R6396 T22192 T22193 punct (,R1
R6397 T22193 T22186 parataxis R1,line
R6398 T22194 T22193 punct ),R1
R6399 T22195 T22186 punct ", ",line
R6400 T22196 T22197 det a,line
R6401 T22197 T22186 appos line,line
R6402 T22198 T22197 amod heterozygous,line
R6403 T22199 T22197 amod targeted,line
R6404 T22200 T22201 compound ES,cell
R6405 T22201 T22197 compound cell,line
R6406 T22202 T22203 punct (,362
R6407 T22203 T22197 parataxis 362,line
R6408 T22204 T22203 punct ),362
R6409 T22205 T22184 punct ", ",from
R6410 T22206 T22184 cc and,from
R6411 T22207 T22184 conj from,from
R6412 T22208 T22209 amod wild,type
R6413 T22209 T22210 nmod type,knockout
R6414 T22210 T22225 nmod knockout,mice
R6415 T22211 T22212 punct (,+
R6416 T22212 T22209 punct +,type
R6417 T22213 T22212 punct +,+
R6418 T22214 T22212 punct /,+
R6419 T22215 T22212 punct ),+
R6420 T22216 T22209 punct ", ",type
R6421 T22217 T22209 conj heterozygous,type
R6422 T22218 T22219 punct (,-
R6423 T22219 T22217 punct -,heterozygous
R6424 T22220 T22219 punct +,-
R6425 T22221 T22219 punct /,-
R6426 T22222 T22219 punct ),-
R6427 T22223 T22217 cc and,heterozygous
R6428 T22224 T22217 conj homozygous,heterozygous
R6429 T22225 T22207 pobj mice,from
R6430 T22226 T22227 punct (,-
R6431 T22227 T22210 punct -,knockout
R6432 T22228 T22227 punct -,-
R6433 T22229 T22227 punct /,-
R6434 T22230 T22227 punct ),-
R6435 T22231 T22174 punct .,analysis
R6436 T22233 T22234 det The,blot
R6437 T22234 T22235 nsubjpass blot,hybridized
R6438 T22236 T22235 auxpass was,hybridized
R6439 T22237 T22235 prep with,hybridized
R6440 T22238 T22239 det an,probe
R6441 T22239 T22237 pobj probe,with
R6442 T22240 T22241 npadvmod Exon,specific
R6443 T22241 T22239 amod specific,probe
R6444 T22242 T22240 nummod 6,Exon
R6445 T22243 T22235 punct .,hybridized
R6446 T22245 T22246 meta C,analysis
R6447 T22247 T22246 punct ", ",analysis
R6448 T22248 T22246 amod Immunohistochemical,analysis
R6449 T22249 T22246 prep of,analysis
R6450 T22250 T22251 amod fresh,frozen
R6451 T22251 T22252 amod frozen,sections
R6452 T22252 T22249 pobj sections,of
R6453 T22253 T22252 compound liver,sections
R6454 T22254 T22252 prep from,sections
R6455 T22255 T22256 nmod control,knockout
R6456 T22256 T22264 nmod knockout,pups
R6457 T22257 T22258 punct (,+
R6458 T22258 T22255 punct +,control
R6459 T22259 T22258 punct +,+
R6460 T22260 T22258 punct /,+
R6461 T22261 T22258 punct ),+
R6462 T22262 T22255 cc and,control
R6463 T22263 T22255 conj E2,control
R6464 T22264 T22254 pobj pups,from
R6465 T22265 T22266 punct (,-
R6466 T22266 T22256 punct -,knockout
R6467 T22267 T22266 punct -,-
R6468 T22268 T22266 punct /,-
R6469 T22269 T22266 punct ),-
R6470 T22270 T22271 amod postnatal,day
R6471 T22271 T22264 nmod day,pups
R6472 T22272 T22271 nummod 1,day
R6473 T22273 T22264 compound mouse,pups
R6474 T22274 T22246 punct .,analysis
R6475 T22276 T22277 nsubjpass Sections,stained
R6476 T22278 T22277 auxpass were,stained
R6477 T22279 T22277 prep for,stained
R6478 T22280 T22279 pobj E2,for
R6479 T22281 T22277 advcl using,stained
R6480 T22282 T22283 det an,antibody
R6481 T22283 T22281 dobj antibody,using
R6482 T22284 T22285 npadvmod E2,specific
R6483 T22285 T22283 amod specific,antibody
R6484 T22286 T22287 punct (,green
R6485 T22287 T22283 parataxis green,antibody
R6486 T22288 T22287 punct ),green
R6487 T22289 T22283 cc and,antibody
R6488 T22290 T22291 det a,stain
R6489 T22291 T22283 conj stain,antibody
R6490 T22292 T22291 amod nuclear,stain
R6491 T22293 T22294 punct (,blue
R6492 T22294 T22291 parataxis blue,stain
R6493 T22295 T22294 punct ),blue
R6494 T22296 T22277 punct .,stained
R6495 T22299 T22300 det the,absence
R6496 T22300 T22298 dobj absence,Note
R6497 T22301 T22300 amod complete,absence
R6498 T22302 T22300 prep of,absence
R6499 T22303 T22304 compound E2,immunoreactivity
R6500 T22304 T22302 pobj immunoreactivity,of
R6501 T22305 T22298 prep in,Note
R6502 T22306 T22307 det the,section
R6503 T22307 T22305 pobj section,in
R6504 T22308 T22307 prep from,section
R6505 T22309 T22310 det the,mouse
R6506 T22310 T22308 pobj mouse,from
R6507 T22311 T22310 compound knockout,mouse
R6508 T22312 T22298 punct .,Note
R6509 T22314 T22315 meta D,observed
R6510 T22316 T22315 punct ", ",observed
R6511 T22317 T22318 amod Similar,results
R6512 T22318 T22315 nsubjpass results,observed
R6513 T22319 T22315 auxpass were,observed
R6514 T22320 T22315 prep upon,observed
R6515 T22321 T22322 amod immunohistochemical,analysis
R6516 T22322 T22320 pobj analysis,upon
R6517 T22323 T22322 prep of,analysis
R6518 T22324 T22325 amod primary,fibroblasts
R6519 T22325 T22323 pobj fibroblasts,of
R6520 T22326 T22325 nmod mouse,fibroblasts
R6521 T22327 T22325 amod embryonic,fibroblasts
R6522 T22328 T22325 punct (,fibroblasts
R6523 T22329 T22325 appos MEFs,fibroblasts
R6524 T22330 T22315 punct ),observed
R6525 T22331 T22315 punct .,observed
R6526 T22334 T22335 mark that,was
R6527 T22335 T22333 ccomp was,Note
R6528 T22336 T22337 det the,signal
R6529 T22337 T22335 nsubj signal,was
R6530 T22338 T22339 advmod readily,detectable
R6531 T22339 T22337 amod detectable,signal
R6532 T22340 T22337 prep for,signal
R6533 T22341 T22340 pobj E2,for
R6534 T22342 T22337 prep in,signal
R6535 T22343 T22344 det the,cells
R6536 T22344 T22342 pobj cells,in
R6537 T22345 T22344 compound control,cells
R6538 T22346 T22335 acomp present,was
R6539 T22347 T22346 prep in,present
R6540 T22348 T22349 det a,pattern
R6541 T22349 T22347 pobj pattern,in
R6542 T22350 T22349 amod characteristic,pattern
R6543 T22351 T22350 prep of,characteristic
R6544 T22352 T22351 pobj mitochondria,of
R6545 T22353 T22352 punct ", ",mitochondria
R6546 T22354 T22355 det the,location
R6547 T22355 T22352 appos location,mitochondria
R6548 T22356 T22355 amod subcellular,location
R6549 T22357 T22355 prep of,location
R6550 T22358 T22357 pobj BCKDH,of
R6551 T22359 T22333 punct .,Note

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T4357 30-37 NCBITaxon:33208 denotes animals
T4358 113-119 NCBITaxon:33208 denotes Animal
T4968 145-149 SO_EXT:0000704 denotes Gene
T4969 172-183 SO_EXT:genomic_DNA denotes genomic DNA
T4970 180-183 CHEBI_SO_EXT:DNA denotes DNA
T4971 211-214 CHEBI_SO_EXT:DNA denotes DNA
T4972 232-248 SO_EXT:0000852 denotes portion of Exons
T4973 264-266 PR_EXT:000006300 denotes E2
T4974 267-271 SO_EXT:0000704 denotes gene
T4975 276-284 SO:0000357 denotes flanking
T4976 285-288 CHEBI_SO_EXT:DNA denotes DNA
T4977 309-317 NCBITaxon:10678 denotes P1 phage
T4978 331-337 SO_EXT:0001026 denotes Genome
T4979 376-381 NCBITaxon:10088 denotes Mouse
T4980 382-384 CL:0002322 denotes ES
T4981 416-427 SO_EXT:sequence_substitution_entity_or_process denotes replacement
T4982 433-437 SO_EXT:0000704 denotes gene
T4983 438-454 SO_EXT:0001644 denotes targeting vector
T4984 475-484 SO_EXT:sequence_substitution_process denotes replacing
T4985 493-494 CHEBI_SO_EXT:base denotes b
T4986 531-546 SO_EXT:0000852 denotes portion of Exon
T4987 560-564 SO_EXT:0000147 denotes Exon
T4988 583-589 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T4989 590-594 SO_EXT:0000704 denotes gene
T4990 623-629 SO_EXT:0000440 denotes vector
T4991 650-659 SO_EXT:engineered_biological_sequence denotes construct
T4992 664-674 SO_EXT:sequence_linearization denotes linearized
T4993 712-720 CL:0002322 denotes ES cells
T4994 715-720 CL_GO_EXT:cell denotes cells
T4995 770-778 CL:0002322 denotes ES cells
T4996 773-778 CL_GO_EXT:cell denotes cells
T4997 798-802 CHEBI:42768 denotes G418
T4998 815-819 UBERON:0000104 denotes Life
T4999 861-872 CHEBI:465284 denotes gancyclovir
T5000 929-935 SO_EXT:sequence_cloned_entity denotes clones
T5001 954-958 SO_EXT:0000704 denotes gene
T5002 1012-1023 SO_EXT:genomic_DNA denotes genomic DNA
T5003 1020-1023 CHEBI_SO_EXT:DNA denotes DNA
T5004 1036-1046 GO:0097617 denotes hybridized
T5005 1055-1059 SO_EXT:0000147 denotes Exon
T5006 1071-1076 CHEBI_SO_EXT:molecular_probe denotes probe
T5007 1102-1106 SO_EXT:0000704 denotes gene
T5008 1117-1126 SO_EXT:engineered_biological_sequence denotes construct
T5009 1147-1155 CL:0002322 denotes ES cells
T5010 1150-1155 CL_GO_EXT:cell denotes cells
T5011 1184-1195 UBERON:0000358 denotes blastocysts
T5012 1216-1220 NCBITaxon:10088 denotes mice
T5013 1333-1342 SO_EXT:wild_type_entity_or_quality denotes wild type
T5014 1344-1345 SO_EXT:normal_or_wild_type_or_present denotes +
T5015 1346-1347 SO_EXT:normal_or_wild_type_or_present denotes +
T5016 1364-1365 SO_EXT:normal_or_wild_type_or_present denotes +
T5017 1366-1367 SO_EXT:sequence_nullness_or_absence denotes -
T5018 1394-1395 SO_EXT:sequence_nullness_or_absence denotes -
T5019 1396-1397 SO_EXT:sequence_nullness_or_absence denotes -
T5020 1399-1403 NCBITaxon:10088 denotes mice
T5021 1425-1426 SO_EXT:normal_or_wild_type_or_present denotes +
T5022 1427-1428 SO_EXT:sequence_nullness_or_absence denotes -
T5023 1429-1437 GO_EXT:breeding denotes breeding
T5024 1487-1491 NCBITaxon:10088 denotes mice
T5025 1530-1537 NCBITaxon:33208 denotes animals
T5026 1582-1589 SO_EXT:0000704 denotes genetic
T5818 3097-3107 SO_EXT:transgenic_entity denotes transgenic
T5819 3108-3112 NCBITaxon:10088 denotes mice
T5820 3122-3131 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T5821 3169-3179 SO_EXT:transgenic_entity denotes transgenic
T5822 3180-3189 SO_EXT:engineered_biological_sequence denotes construct
T5823 3196-3198 PR_EXT:000006300 denotes E2
T5824 3205-3214 SO_EXT:0000902 denotes transgene
T5825 3228-3240 CHEBI:27902 denotes tetracycline
T5826 3252-3256 NCBITaxon:10359 denotes hCMV
T5827 3260-3268 SO_EXT:0000167 denotes promoter
T5828 3288-3300 CHEBI:27902 denotes tetracycline
T5829 3340-3344 NCBITaxon:10359 denotes hCMV
T5830 3345-3353 SO_EXT:0000167 denotes promoter
T5831 3413-3419 SO_EXT:0000188 denotes intron
T5832 3451-3458 SO_EXT:0000673 denotes message
T5833 3451-3458 CHEBI:33695 denotes message
T5834 3473-3483 GO:0010467 denotes expression
T5835 3501-3510 SO:0000993 denotes consensus
T5836 3511-3519 SO_EXT:biological_sequence denotes sequence
T5837 3527-3543 SO_EXT:0000318 denotes initiation codon
T5838 3556-3567 GO:0006412 denotes translation
T5839 3578-3583 NCBITaxon:9606 denotes human
T5840 3584-3586 PR_EXT:000006300 denotes E2
T5841 3587-3591 SO_EXT:cDNA denotes cDNA
T5842 3607-3615 SO_EXT:sequence_alteration_process denotes modified
T5843 3632-3639 CHEBI_SO_EXT:alanine denotes alanine
T5844 3661-3666 PR_EXT:000000084 denotes c-myc
T5845 3667-3674 CHEBI_SO_EXT:epitope denotes epitope
T5846 3675-3678 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes tag
T5847 3686-3702 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes carboxy terminus
T5848 3735-3739 NCBITaxon:10633 denotes SV40
T5849 3745-3760 GO:0043631 denotes polyadenylation
T5850 3745-3769 SO_EXT:0000610 denotes polyadenylation sequence
T5851 3792-3796 CHEBI_SO_EXT:mRNA denotes mRNA
T5852 3811-3822 GO:0006412 denotes translation
T5853 3834-3835 CHEBI_SO_EXT:base denotes b
T5854 3840-3842 PR_EXT:000006300 denotes E2
T5855 3843-3852 SO_EXT:0000902 denotes transgene
T5856 3871-3877 SO_EXT:0000440 denotes vector
T5857 3878-3887 SO_EXT:biological_sequence denotes sequences
T5858 3922-3927 PR_EXT:P23940 denotes BamHI
T5859 3984-3989 NCBITaxon:10088 denotes mouse
T5860 3990-3997 UBERON:0000922 denotes embryos
T5861 4005-4015 SO_EXT:transgenic_entity denotes transgenic
T5862 4112-4123 SO_EXT:genomic_DNA denotes Genomic DNA
T5863 4120-4123 CHEBI_SO_EXT:DNA denotes DNA
T5864 4133-4137 UBERON:0002415 denotes tail
T5865 4141-4145 NCBITaxon:10088 denotes mice
T5866 4220-4233 GO:0097617 denotes hybridization
T5867 4245-4247 SO_EXT:0000028 denotes bp
T5868 4248-4253 CHEBI_SO_EXT:molecular_probe denotes probe
T5869 4271-4275 NCBITaxon:10633 denotes SV40
T5870 4295-4297 PR_EXT:000006300 denotes E2
T5871 4298-4307 SO_EXT:0000902 denotes transgene
T6401 4342-4348 NCBITaxon:39107 denotes murine
T6402 4371-4373 PR_EXT:000006300 denotes E2
T6403 4374-4384 SO_EXT:transgenic_entity denotes transgenic
T6404 4405-4410 GO:0007618 denotes mated
T6405 4428-4432 NCBITaxon:10088 denotes mice
T6406 4469-4471 PR_EXT:000006300 denotes E2
T6407 4489-4492 PR_EXT:000005308 denotes LAP
T6408 4497-4506 SO_EXT:0000902 denotes transgene
T6409 4508-4510 SO_EXT:transgenic_entity denotes Tg
T6410 4617-4625 GO_EXT:breeding denotes breeding
T6411 4629-4636 NCBITaxon:33208 denotes animals
T6412 4669-4679 SO_EXT:0000902 denotes transgenes
T6413 4727-4731 NCBITaxon:10088 denotes mice
T6414 4750-4759 SO_EXT:genotype_or_entity_with_genotype denotes genotypes
T6415 4775-4782 NCBITaxon:33208 denotes animals
T6416 4848-4858 SO_EXT:0000902 denotes transgenes
T6417 4877-4885 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T6418 4894-4900 SO_EXT:sequence_rescue_process denotes rescue
T6419 4902-4910 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T6420 4933-4941 SO_EXT:sequence_rescue_process denotes rescuing
T6421 4946-4954 PATO_UBERON_EXT:neonate_or_newborn denotes neonatal
T6422 4955-4961 GO_EXT:fatality_or_lethality denotes lethal
T6423 5036-5043 NCBITaxon:33208 denotes animals
T6424 5058-5068 SO_EXT:0000902 denotes transgenes
T6425 5094-5109 UBERON:0007221 denotes neonatal period
T6662 5127-5131 NCBITaxon:10088 denotes mice
T6663 5174-5186 SO_EXT:genomic_DNA denotes Genomic DNAs
T6664 5182-5186 CHEBI_SO_EXT:DNA denotes DNAs
T6665 5201-5205 UBERON:0002415 denotes tail
T6666 5246-5264 GO_EXT:0015666 denotes restriction enzyme
T6667 5258-5264 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T6668 5287-5294 CHEBI:2511 denotes agarose
T6669 5327-5332 CHEBI:25614 denotes nylon
T7154 5402-5407 NCBITaxon:10088 denotes mouse
T7155 5408-5417 UBERON:0000922 denotes embryonic
T7156 5418-5429 CL:0000057 denotes fibroblasts
T7157 5449-5458 UBERON:0000922 denotes embryonic
T7158 5501-5512 CL:0000057 denotes Fibroblasts
T7159 5549-5565 CHEBI_EXT:paraformaldehye denotes paraformaldehyde
T7160 5609-5625 CHEBI_EXT:paraformaldehye denotes paraformaldehyde
T7161 5642-5653 CHEBI:9750 denotes Triton X100
T7162 5729-5736 CHEBI_SO_EXT:glycine denotes glycine
T7163 5746-5752 CHEBI_CHMO_EXT:buffer_solution denotes Buffer
T7164 5801-5805 NCBITaxon:9925 denotes goat
T7165 5806-5809 GO:0071735 denotes IgG
T7166 5863-5869 CHEBI_CHMO_EXT:buffer_solution denotes Buffer
T7167 5873-5878 CL_GO_EXT:cell denotes cells
T7168 5910-5912 PR_EXT:000006300 denotes E2
T7169 5926-5931 UBERON:0001977 denotes serum
T7170 5976-5982 CHEBI_CHMO_EXT:buffer_solution denotes Buffer
T7171 6027-6034 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T7172 6042-6050 GO:0042571 denotes antibody
T7173 6068-6073 CL_GO_EXT:cell denotes cells
T7174 6117-6123 CHEBI_CHMO_EXT:buffer_solution denotes Buffer
T7175 6146-6154 CHEBI_EXT:poly_vinyl_alcohol denotes gelvatol
T7176 6182-6188 UBERON:0002107 denotes livers
T7177 6194-6201 PATO_UBERON_EXT:neonate_or_newborn denotes newborn
T7178 6202-6206 NCBITaxon_UBERON_EXT:pup denotes pups
T7179 6254-6270 CHEBI_EXT:paraformaldehye denotes paraformaldehyde
T7180 6327-6335 CHEBI_EXT:nitrogen denotes nitrogen
T7181 6343-6353 CHEBI:30362 denotes isopentane
T7182 6418-6423 CL_GO_EXT:cell denotes cells
T7534 6481-6491 CHEBI_SO_EXT:amino_acid denotes Amino acid
T7535 6501-6506 UBERON:0000178 denotes Blood
T7536 6543-6548 UBERON:0001825 denotes sinus
T7537 6552-6561 UBERON:0003481 denotes tail vein
T7538 6565-6569 NCBITaxon:10088 denotes mice
T7539 6619-6624 UBERON:0000178 denotes blood
T7540 6625-6635 CHEBI_SO_EXT:amino_acid denotes amino acid
T7541 6652-6657 GO:0007567 denotes natal
T7542 6687-6691 CHEBI:22918 denotes BCAA
T7543 6702-6713 CHEBI_SO_EXT:amino_acid denotes amino acids
T7544 6717-6722 UBERON:0000178 denotes blood
T8070 6832-6838 UBERON:0002107 denotes Livers
T8071 6870-6878 CHEBI_EXT:nitrogen denotes nitrogen
T8072 6916-6922 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T8073 6930-6936 UBERON:0002107 denotes livers
T8074 6987-6994 CHEBI:17992 denotes sucrose
T8075 7002-7006 CHEBI:9754 denotes Tris
T8076 7007-7010 CHEBI:17883 denotes HC1
T8077 7020-7025 UBERON:0002107 denotes Liver
T8078 7167-7173 UBERON:0000479 denotes tissue
T8079 7234-7239 UBERON:0002107 denotes liver
T8080 7240-7246 UBERON:0000479 denotes tissue
T8081 7266-7273 PATO_UBERON_EXT:neonate_or_newborn denotes newborn
T8082 7274-7278 NCBITaxon_UBERON_EXT:pup denotes pups
T8083 7327-7334 GO_EXT:biological_release denotes release
T8084 7340-7343 CHEBI:16526 denotes CO2
T8085 7349-7355 _FRAGMENT denotes α-keto
T8086 7364-7375 CHEBI:17865 denotes isocaproate
T8087 7359-7362 CHEBI:36927 denotes 14C
T8088 7428-7435 CHEBI:60004 denotes mixture
T8089 7472-7491 CHEBI_EXT:potassium_phosphate denotes potassium phosphate
T8090 7492-7498 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T8091 7516-7533 CHEBI:17865 denotes α-ketoisocaproate
T8092 7543-7548 CHEBI:15346 denotes CoASH
T8093 7558-7579 CHEBI:9532 denotes thiamin pyrophosphate
T8094 7586-7590 CHEBI:15846 denotes NAD+
T8095 7597-7611 CHEBI:18320 denotes dithiothreitol
T8096 7618-7622 CHEBI:18420 denotes Mg2+
T8097 7653-7659 _FRAGMENT denotes α-keto
T8098 7668-7679 CHEBI:17865 denotes isocaproate
T8099 7663-7666 CHEBI:36927 denotes 14C
T8100 7696-7701 UBERON:0002107 denotes liver
T8101 7721-7728 CHEBI_PR_EXT:protein denotes protein
T8102 7777-7780 CHEBI:16526 denotes CO2
T8103 7796-7805 CHEBI:24651 denotes hydroxide
T8104 7809-7816 CHEBI:31264 denotes Hyamine
T8787 7912-7919 CHEBI_PR_EXT:protein denotes Protein
T8788 7960-7965 UBERON:0002107 denotes liver
T8789 8006-8014 SO_EXT:wild_type_entity_or_quality denotes wildtype
T8790 8038-8048 SO_EXT:transgenic_entity denotes transgenic
T8791 8049-8053 NCBITaxon:10088 denotes mice
T8792 8055-8062 CHEBI_PR_EXT:protein denotes Protein
T8793 8123-8126 CHEBI:8984 denotes SDS
T8794 8170-8181 GO_EXT:biological_movement_or_translocation_process denotes transferred
T8795 8185-8189 CHEBI:53250 denotes PVDF
T8796 8268-8270 PR_EXT:000006300 denotes E2
T8797 8271-8278 CHEBI_PR_EXT:protein denotes protein
T8798 8296-8302 NCBITaxon:9986 denotes rabbit
T8799 8303-8305 PR_EXT:000006300 denotes E2
T8800 8310-8314 UBERON:0001977 denotes sera
T8801 8345-8350 NCBITaxon:10088 denotes mouse
T8802 8364-8369 NCBITaxon:9606 denotes human
T8803 8379-8390 PR_EXT:000006300 denotes E2 subunits
T8804 8479-8485 NCBITaxon:9986 denotes rabbit
T8805 8491-8496 PR_EXT:000000084 denotes c-myc
T8806 8497-8500 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes tag
T8807 8501-8509 GO:0042571 denotes antibody
T8808 8596-8604 GO:0042571 denotes antibody
T8809 8609-8616 PR_EXT:000003676 denotes β-actin
T8810 8695-8699 NCBITaxon:9925 denotes goat
T8811 8705-8711 NCBITaxon:9986 denotes rabbit
T8812 8722-8730 GO:0042571 denotes antibody
T8813 8731-8741 MOP:0000779 denotes conjugated
T8814 8745-8756 NCBITaxon:3704 denotes horseradish
T8815 8757-8767 GO_EXT:0004601 denotes peroxidase
T8816 8885-8892 CHEBI_EXT:33893 denotes reagent
T9138 8973-8977 CHEBI:22918 denotes BCAA
T9139 8988-8994 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T9140 9100-9109 SO_EXT:genotype_or_entity_with_genotype denotes genotypes
T21984 1612-1614 PR_EXT:000006300 denotes E2
T21985 1615-1619 SO_EXT:0000704 denotes gene
T21986 1629-1634 NCBITaxon:10088 denotes mouse
T21987 1650-1654 SO_EXT:0000704 denotes Gene
T21988 1697-1699 PR_EXT:000006300 denotes E2
T21989 1709-1714 NCBITaxon:10088 denotes mouse
T21990 1715-1723 CL:0002322 denotes ES cells
T21991 1718-1723 CL_GO_EXT:cell denotes cells
T21992 1739-1748 SO_EXT:engineered_biological_sequence denotes construct
T21993 1765-1771 SO_EXT:sequence_deletion_process denotes delete
T21994 1778-1779 CHEBI_SO_EXT:base denotes b
T21995 1783-1791 SO_EXT:biological_sequence denotes sequence
T21996 1809-1821 SO_EXT:0000852 denotes site in Exon
T21997 1843-1849 SO_EXT:0000188 denotes intron
T21998 1857-1866 SO_EXT:wild_type_entity_or_quality denotes wild type
T21999 1867-1869 PR_EXT:000006300 denotes E2
T22000 1870-1874 SO_EXT:0000704 denotes gene
T22001 1892-1893 CHEBI_SO_EXT:base denotes b
T22002 1899-1919 SO_EXT:0000412 denotes restriction fragment
T22003 1925-1935 GO:0097617 denotes hybridizes
T22004 1943-1947 SO_EXT:0000147 denotes Exon
T22005 1959-1964 CHEBI_SO_EXT:molecular_probe denotes probe
T22006 1987-1989 PR_EXT:000006300 denotes E2
T22007 2012-2013 CHEBI_SO_EXT:base denotes b
T22008 2019-2039 SO_EXT:0000412 denotes restriction fragment
T22009 2045-2055 GO:0097617 denotes hybridizes
T22010 2068-2073 CHEBI_SO_EXT:molecular_probe denotes probe
T22011 2089-2094 CHEBI_SO_EXT:molecular_probe denotes probe
T22012 2137-2153 SO_EXT:0001644 denotes targeting vector
T22013 2184-2200 SO_EXT:0001644 denotes targeting vector
T22014 2245-2256 SO_EXT:genomic_DNA denotes genomic DNA
T22015 2253-2256 CHEBI_SO_EXT:DNA denotes DNA
T22016 2283-2292 SO_EXT:wild_type_entity_or_quality denotes wild type
T22017 2293-2300 CL:0002322 denotes ES cell
T22018 2296-2300 CL_GO_EXT:cell denotes cell
T22019 2336-2343 CL:0002322 denotes ES cell
T22020 2339-2343 CL_GO_EXT:cell denotes cell
T22021 2365-2374 SO_EXT:wild_type_entity_or_quality denotes wild type
T22022 2376-2377 SO_EXT:normal_or_wild_type_or_present denotes +
T22023 2378-2379 SO_EXT:normal_or_wild_type_or_present denotes +
T22024 2396-2397 SO_EXT:normal_or_wild_type_or_present denotes +
T22025 2398-2399 SO_EXT:sequence_nullness_or_absence denotes -
T22026 2426-2427 SO_EXT:sequence_nullness_or_absence denotes -
T22027 2428-2429 SO_EXT:sequence_nullness_or_absence denotes -
T22028 2431-2435 NCBITaxon:10088 denotes mice
T22029 2450-2460 GO:0097617 denotes hybridized
T22030 2469-2473 SO_EXT:0000147 denotes Exon
T22031 2485-2490 CHEBI_SO_EXT:molecular_probe denotes probe
T22032 2540-2545 UBERON:0002107 denotes liver
T22033 2569-2570 SO_EXT:normal_or_wild_type_or_present denotes +
T22034 2571-2572 SO_EXT:normal_or_wild_type_or_present denotes +
T22035 2578-2580 PR_EXT:000006300 denotes E2
T22036 2591-2592 SO_EXT:sequence_nullness_or_absence denotes -
T22037 2593-2594 SO_EXT:sequence_nullness_or_absence denotes -
T22038 2600-2605 GO:0007567 denotes natal
T22039 2612-2617 NCBITaxon:10088 denotes mouse
T22040 2618-2622 NCBITaxon_UBERON_EXT:pup denotes pups
T22041 2650-2652 PR_EXT:000006300 denotes E2
T22042 2662-2664 PR_EXT:000006300 denotes E2
T22043 2674-2682 GO:0042571 denotes antibody
T22044 2697-2704 GO:0005634 denotes nuclear
T22045 2748-2750 PR_EXT:000006300 denotes E2
T22046 2801-2806 NCBITaxon:10088 denotes mouse
T22047 2886-2891 NCBITaxon:10088 denotes mouse
T22048 2892-2901 UBERON:0000922 denotes embryonic
T22049 2902-2913 CL:0000057 denotes fibroblasts
T22050 2966-2968 PR_EXT:000006300 denotes E2
T22051 2984-2989 CL_GO_EXT:cell denotes cells
T22052 3033-3045 GO:0005739 denotes mitochondria
T22053 3051-3062 GO_UBERON_EXT:cellular_component_or_cell_part denotes subcellular
T22054 3054-3062 CL_GO_EXT:cell denotes cellular
R2280 T8086 T8085 _lexicallyChainedTo isocaproate,α-keto
R2281 T8098 T8097 _lexicallyChainedTo isocaproate,α-keto

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T4355 30-37 NCBITaxon:33208 denotes animals
T4356 113-119 NCBITaxon:33208 denotes Animal
T4935 145-149 SO:0000704 denotes Gene
T4936 172-179 SO:0001026 denotes genomic
T4937 232-248 SO:0000852 denotes portion of Exons
T4938 264-266 PR:000006300 denotes E2
T4939 267-271 SO:0000704 denotes gene
T4940 276-284 SO:0000357 denotes flanking
T4941 309-317 NCBITaxon:10678 denotes P1 phage
T4942 331-337 SO:0001026 denotes Genome
T4943 376-381 NCBITaxon:10088 denotes Mouse
T4944 382-384 CL:0002322 denotes ES
T4945 433-437 SO:0000704 denotes gene
T4946 438-454 SO:0001644 denotes targeting vector
T4947 531-546 SO:0000852 denotes portion of Exon
T4948 560-564 SO:0000147 denotes Exon
T4949 590-594 SO:0000704 denotes gene
T4950 623-629 SO:0000440 denotes vector
T4951 712-720 CL:0002322 denotes ES cells
T4952 770-778 CL:0002322 denotes ES cells
T4953 798-802 CHEBI:42768 denotes G418
T4954 815-819 UBERON:0000104 denotes Life
T4955 861-872 CHEBI:465284 denotes gancyclovir
T4956 954-958 SO:0000704 denotes gene
T4957 1012-1019 SO:0001026 denotes genomic
T4958 1036-1046 GO:0097617 denotes hybridized
T4959 1055-1059 SO:0000147 denotes Exon
T4960 1102-1106 SO:0000704 denotes gene
T4961 1147-1155 CL:0002322 denotes ES cells
T4962 1184-1195 UBERON:0000358 denotes blastocysts
T4963 1216-1220 NCBITaxon:10088 denotes mice
T4964 1399-1403 NCBITaxon:10088 denotes mice
T4965 1487-1491 NCBITaxon:10088 denotes mice
T4966 1530-1537 NCBITaxon:33208 denotes animals
T4967 1582-1589 SO:0000704 denotes genetic
T5781 3108-3112 NCBITaxon:10088 denotes mice
T5782 3196-3198 PR:000006300 denotes E2
T5783 3205-3214 SO:0000902 denotes transgene
T5784 3228-3240 CHEBI:27902 denotes tetracycline
T5785 3252-3256 NCBITaxon:10359 denotes hCMV
T5786 3260-3268 SO:0000167 denotes promoter
T5787 3288-3300 CHEBI:27902 denotes tetracycline
T5788 3340-3344 NCBITaxon:10359 denotes hCMV
T5789 3345-3353 SO:0000167 denotes promoter
T5790 3413-3419 SO:0000188 denotes intron
T5791 3451-3458 SO:0000673 denotes message
T5792 3451-3458 CHEBI:33695 denotes message
T5793 3473-3483 GO:0010467 denotes expression
T5794 3501-3510 SO:0000993 denotes consensus
T5795 3527-3543 SO:0000318 denotes initiation codon
T5796 3556-3567 GO:0006412 denotes translation
T5797 3578-3583 NCBITaxon:9606 denotes human
T5798 3584-3586 PR:000006300 denotes E2
T5799 3661-3666 PR:000000084 denotes c-myc
T5800 3735-3739 NCBITaxon:10633 denotes SV40
T5801 3745-3760 GO:0043631 denotes polyadenylation
T5802 3745-3769 SO:0000610 denotes polyadenylation sequence
T5803 3811-3822 GO:0006412 denotes translation
T5804 3840-3842 PR:000006300 denotes E2
T5805 3843-3852 SO:0000902 denotes transgene
T5806 3871-3877 SO:0000440 denotes vector
T5807 3922-3927 PR:P23940 denotes BamHI
T5808 3984-3989 NCBITaxon:10088 denotes mouse
T5809 3990-3997 UBERON:0000922 denotes embryos
T5810 4112-4119 SO:0001026 denotes Genomic
T5811 4133-4137 UBERON:0002415 denotes tail
T5812 4141-4145 NCBITaxon:10088 denotes mice
T5813 4220-4233 GO:0097617 denotes hybridization
T5814 4245-4247 SO:0000028 denotes bp
T5815 4271-4275 NCBITaxon:10633 denotes SV40
T5816 4295-4297 PR:000006300 denotes E2
T5817 4298-4307 SO:0000902 denotes transgene
T6386 4342-4348 NCBITaxon:39107 denotes murine
T6387 4371-4373 PR:000006300 denotes E2
T6388 4405-4410 GO:0007618 denotes mated
T6389 4428-4432 NCBITaxon:10088 denotes mice
T6390 4469-4471 PR:000006300 denotes E2
T6391 4489-4492 PR:000005308 denotes LAP
T6392 4497-4506 SO:0000902 denotes transgene
T6393 4629-4636 NCBITaxon:33208 denotes animals
T6394 4669-4679 SO:0000902 denotes transgenes
T6395 4727-4731 NCBITaxon:10088 denotes mice
T6396 4775-4782 NCBITaxon:33208 denotes animals
T6397 4848-4858 SO:0000902 denotes transgenes
T6398 5036-5043 NCBITaxon:33208 denotes animals
T6399 5058-5068 SO:0000902 denotes transgenes
T6400 5094-5109 UBERON:0007221 denotes neonatal period
T6657 5127-5131 NCBITaxon:10088 denotes mice
T6658 5174-5181 SO:0001026 denotes Genomic
T6659 5201-5205 UBERON:0002415 denotes tail
T6660 5287-5294 CHEBI:2511 denotes agarose
T6661 5327-5332 CHEBI:25614 denotes nylon
T7141 5402-5407 NCBITaxon:10088 denotes mouse
T7142 5408-5417 UBERON:0000922 denotes embryonic
T7143 5418-5429 CL:0000057 denotes fibroblasts
T7144 5449-5458 UBERON:0000922 denotes embryonic
T7145 5501-5512 CL:0000057 denotes Fibroblasts
T7146 5642-5653 CHEBI:9750 denotes Triton X100
T7147 5801-5805 NCBITaxon:9925 denotes goat
T7148 5806-5809 GO:0071735 denotes IgG
T7149 5910-5912 PR:000006300 denotes E2
T7150 5926-5931 UBERON:0001977 denotes serum
T7151 6042-6050 GO:0042571 denotes antibody
T7152 6182-6188 UBERON:0002107 denotes livers
T7153 6343-6353 CHEBI:30362 denotes isopentane
T7526 6501-6506 UBERON:0000178 denotes Blood
T7527 6543-6548 UBERON:0001825 denotes sinus
T7528 6552-6561 UBERON:0003481 denotes tail vein
T7529 6565-6569 NCBITaxon:10088 denotes mice
T7530 6619-6624 UBERON:0000178 denotes blood
T7531 6652-6657 GO:0007567 denotes natal
T7532 6687-6691 CHEBI:22918 denotes BCAA
T7533 6717-6722 UBERON:0000178 denotes blood
T8043 6832-6838 UBERON:0002107 denotes Livers
T8044 6930-6936 UBERON:0002107 denotes livers
T8045 6987-6994 CHEBI:17992 denotes sucrose
T8046 7002-7006 CHEBI:9754 denotes Tris
T8047 7007-7010 CHEBI:17883 denotes HC1
T8048 7020-7025 UBERON:0002107 denotes Liver
T8049 7167-7173 UBERON:0000479 denotes tissue
T8050 7234-7239 UBERON:0002107 denotes liver
T8051 7240-7246 UBERON:0000479 denotes tissue
T8052 7340-7343 CHEBI:16526 denotes CO2
T8053 7349-7355 _FRAGMENT denotes α-keto
T8054 7364-7375 CHEBI:17865 denotes isocaproate
T8055 7359-7362 CHEBI:36927 denotes 14C
T8056 7428-7435 CHEBI:60004 denotes mixture
T8057 7516-7533 CHEBI:17865 denotes α-ketoisocaproate
T8058 7543-7548 CHEBI:15346 denotes CoASH
T8059 7558-7579 CHEBI:9532 denotes thiamin pyrophosphate
T8060 7586-7590 CHEBI:15846 denotes NAD+
T8061 7597-7611 CHEBI:18320 denotes dithiothreitol
T8062 7618-7622 CHEBI:18420 denotes Mg2+
T8063 7653-7659 _FRAGMENT denotes α-keto
T8064 7668-7679 CHEBI:17865 denotes isocaproate
T8065 7663-7666 CHEBI:36927 denotes 14C
T8066 7696-7701 UBERON:0002107 denotes liver
T8067 7777-7780 CHEBI:16526 denotes CO2
T8068 7796-7805 CHEBI:24651 denotes hydroxide
T8069 7809-7816 CHEBI:31264 denotes Hyamine
T8765 7960-7965 UBERON:0002107 denotes liver
T8766 8049-8053 NCBITaxon:10088 denotes mice
T8767 8123-8126 CHEBI:8984 denotes SDS
T8768 8185-8189 CHEBI:53250 denotes PVDF
T8769 8268-8270 PR:000006300 denotes E2
T8770 8296-8302 NCBITaxon:9986 denotes rabbit
T8771 8303-8305 PR:000006300 denotes E2
T8772 8310-8314 UBERON:0001977 denotes sera
T8773 8345-8350 NCBITaxon:10088 denotes mouse
T8774 8364-8369 NCBITaxon:9606 denotes human
T8775 8379-8390 PR:000006300 denotes E2 subunits
T8776 8479-8485 NCBITaxon:9986 denotes rabbit
T8777 8491-8496 PR:000000084 denotes c-myc
T8778 8501-8509 GO:0042571 denotes antibody
T8779 8596-8604 GO:0042571 denotes antibody
T8780 8609-8616 PR:000003676 denotes β-actin
T8781 8695-8699 NCBITaxon:9925 denotes goat
T8782 8705-8711 NCBITaxon:9986 denotes rabbit
T8783 8722-8730 GO:0042571 denotes antibody
T8784 8731-8741 MOP:0000779 denotes conjugated
T8785 8745-8756 NCBITaxon:3704 denotes horseradish
T8786 8885-8892 CHEBI:33893 denotes reagent
T9137 8973-8977 CHEBI:22918 denotes BCAA
T21944 1612-1614 PR:000006300 denotes E2
T21945 1615-1619 SO:0000704 denotes gene
T21946 1629-1634 NCBITaxon:10088 denotes mouse
T21947 1650-1654 SO:0000704 denotes Gene
T21948 1697-1699 PR:000006300 denotes E2
T21949 1709-1714 NCBITaxon:10088 denotes mouse
T21950 1715-1723 CL:0002322 denotes ES cells
T21951 1809-1821 SO:0000852 denotes site in Exon
T21952 1843-1849 SO:0000188 denotes intron
T21953 1867-1869 PR:000006300 denotes E2
T21954 1870-1874 SO:0000704 denotes gene
T21955 1899-1919 SO:0000412 denotes restriction fragment
T21956 1925-1935 GO:0097617 denotes hybridizes
T21957 1943-1947 SO:0000147 denotes Exon
T21958 1987-1989 PR:000006300 denotes E2
T21959 2019-2039 SO:0000412 denotes restriction fragment
T21960 2045-2055 GO:0097617 denotes hybridizes
T21961 2137-2153 SO:0001644 denotes targeting vector
T21962 2184-2200 SO:0001644 denotes targeting vector
T21963 2245-2252 SO:0001026 denotes genomic
T21964 2293-2300 CL:0002322 denotes ES cell
T21965 2336-2343 CL:0002322 denotes ES cell
T21966 2431-2435 NCBITaxon:10088 denotes mice
T21967 2450-2460 GO:0097617 denotes hybridized
T21968 2469-2473 SO:0000147 denotes Exon
T21969 2540-2545 UBERON:0002107 denotes liver
T21970 2578-2580 PR:000006300 denotes E2
T21971 2600-2605 GO:0007567 denotes natal
T21972 2612-2617 NCBITaxon:10088 denotes mouse
T21973 2650-2652 PR:000006300 denotes E2
T21974 2662-2664 PR:000006300 denotes E2
T21975 2674-2682 GO:0042571 denotes antibody
T21976 2697-2704 GO:0005634 denotes nuclear
T21977 2748-2750 PR:000006300 denotes E2
T21978 2801-2806 NCBITaxon:10088 denotes mouse
T21979 2886-2891 NCBITaxon:10088 denotes mouse
T21980 2892-2901 UBERON:0000922 denotes embryonic
T21981 2902-2913 CL:0000057 denotes fibroblasts
T21982 2966-2968 PR:000006300 denotes E2
T21983 3033-3045 GO:0005739 denotes mitochondria
R2278 T8054 T8053 _lexicallyChainedTo isocaproate,α-keto
R2279 T8064 T8063 _lexicallyChainedTo isocaproate,α-keto