Id |
Subject |
Object |
Predicate |
Lexical cue |
T20585 |
898-899 |
. |
denotes |
. |
T20584 |
886-888 |
VB |
denotes |
be |
T20583 |
881-885 |
RB |
denotes |
also |
T20582 |
889-898 |
VBN |
denotes |
regulated |
T20581 |
877-880 |
MD |
denotes |
can |
T20580 |
873-876 |
CC |
denotes |
and |
T20579 |
864-872 |
NN |
denotes |
survival |
T20578 |
860-863 |
IN |
denotes |
for |
T20577 |
854-859 |
VBP |
denotes |
allow |
T20576 |
849-853 |
WDT |
denotes |
that |
T20575 |
843-848 |
NNS |
denotes |
lines |
T20574 |
838-842 |
VB |
denotes |
find |
T20573 |
835-837 |
TO |
denotes |
to |
T20572 |
825-827 |
VB |
denotes |
be |
T20571 |
821-824 |
MD |
denotes |
may |
T20570 |
815-820 |
NNS |
denotes |
lines |
T20569 |
804-814 |
JJ |
denotes |
additional |
T20568 |
801-803 |
IN |
denotes |
of |
T20567 |
828-834 |
VBN |
denotes |
needed |
T20566 |
791-800 |
NN |
denotes |
Screening |
T20565 |
790-899 |
sentence |
denotes |
Screening of additional lines may be needed to find lines that allow for survival and can also be regulated. |
T20564 |
789-790 |
. |
denotes |
. |
T20563 |
779-789 |
NN |
denotes |
expression |
T20562 |
769-778 |
VBN |
denotes |
regulated |
T20561 |
765-768 |
IN |
denotes |
for |
T20560 |
754-764 |
JJ |
denotes |
permissive |
T20559 |
750-753 |
RB |
denotes |
not |
T20558 |
735-745 |
NNS |
denotes |
transgenes |
T20557 |
731-734 |
DT |
denotes |
the |
T20556 |
728-730 |
IN |
denotes |
of |
T20555 |
711-722 |
NN |
denotes |
integration |
T20554 |
723-727 |
NN |
denotes |
site |
T20553 |
707-710 |
DT |
denotes |
the |
T20552 |
746-749 |
VBD |
denotes |
was |
T20551 |
702-706 |
IN |
denotes |
that |
T20550 |
690-701 |
JJ |
denotes |
conceivable |
T20549 |
687-689 |
VBZ |
denotes |
is |
T20548 |
684-686 |
PRP |
denotes |
It |
T20547 |
683-790 |
sentence |
denotes |
It is conceivable that the integration site of the transgenes was not permissive for regulated expression. |
T20546 |
682-683 |
. |
denotes |
. |
T20545 |
675-682 |
JJ |
denotes |
unknown |
T20544 |
660-671 |
NN |
denotes |
doxycycline |
T20543 |
657-659 |
IN |
denotes |
to |
T20542 |
644-656 |
JJ |
denotes |
unresponsive |
T20541 |
639-643 |
VBD |
denotes |
were |
T20540 |
629-632 |
CD |
denotes |
two |
T20539 |
633-638 |
NNS |
denotes |
lines |
T20538 |
623-628 |
DT |
denotes |
these |
T20537 |
672-674 |
VBZ |
denotes |
is |
T20536 |
616-622 |
NN |
denotes |
reason |
T20535 |
612-615 |
DT |
denotes |
The |
T20534 |
611-683 |
sentence |
denotes |
The reason these two lines were unresponsive to doxycycline is unknown. |
T20533 |
610-611 |
. |
denotes |
. |
T20532 |
609-610 |
-RRB- |
denotes |
) |
T20531 |
600-603 |
RB |
denotes |
not |
T20530 |
595-599 |
NNS |
denotes |
data |
T20529 |
604-609 |
VBN |
denotes |
shown |
T20528 |
594-595 |
-LRB- |
denotes |
( |
T20527 |
587-593 |
VBN |
denotes |
tested |
T20526 |
570-580 |
JJ |
denotes |
transgenic |
T20525 |
566-569 |
CD |
denotes |
two |
T20524 |
581-586 |
NNS |
denotes |
lines |
T20523 |
562-565 |
DT |
denotes |
the |
T20522 |
559-561 |
IN |
denotes |
of |
T20521 |
552-558 |
DT |
denotes |
either |
T20520 |
549-551 |
IN |
denotes |
in |
T20519 |
537-548 |
NN |
denotes |
doxycycline |
T20518 |
535-537 |
, |
denotes |
, |
T20517 |
514-526 |
NN |
denotes |
tetracycline |
T20516 |
507-513 |
JJ |
denotes |
potent |
T20515 |
527-535 |
NN |
denotes |
analogue |
T20514 |
503-506 |
DT |
denotes |
the |
T20513 |
500-502 |
IN |
denotes |
by |
T20512 |
480-490 |
NN |
denotes |
expression |
T20511 |
477-479 |
NN |
denotes |
E2 |
T20510 |
471-476 |
JJ |
denotes |
human |
T20509 |
491-499 |
NN |
denotes |
cassette |
T20508 |
467-470 |
DT |
denotes |
the |
T20507 |
463-466 |
RP |
denotes |
off |
T20506 |
445-457 |
RB |
denotes |
consistently |
T20505 |
458-462 |
VB |
denotes |
turn |
T20504 |
442-444 |
TO |
denotes |
to |
T20503 |
435-441 |
JJ |
denotes |
unable |
T20502 |
427-429 |
PRP |
denotes |
we |
T20501 |
425-427 |
, |
denotes |
, |
T20500 |
430-434 |
VBD |
denotes |
were |
T20499 |
418-425 |
RB |
denotes |
However |
T20498 |
417-611 |
sentence |
denotes |
However, we were unable to consistently turn off the human E2 expression cassette by the potent tetracycline analogue, doxycycline in either of the two transgenic lines tested (data not shown). |
T20497 |
416-417 |
. |
denotes |
. |
T20496 |
398-406 |
VBG |
denotes |
rescuing |
T20495 |
407-416 |
NN |
denotes |
transgene |
T20494 |
394-397 |
DT |
denotes |
the |
T20493 |
390-393 |
RP |
denotes |
off |
T20492 |
382-389 |
VBG |
denotes |
turning |
T20491 |
379-381 |
IN |
denotes |
by |
T20490 |
365-367 |
POS |
denotes |
's |
T20489 |
368-378 |
NN |
denotes |
discretion |
T20488 |
353-365 |
NN |
denotes |
investigator |
T20487 |
349-352 |
DT |
denotes |
the |
T20486 |
346-348 |
IN |
denotes |
at |
T20485 |
328-335 |
JJ |
denotes |
classic |
T20484 |
336-345 |
NN |
denotes |
phenotype |
T20483 |
324-327 |
DT |
denotes |
the |
T20482 |
321-323 |
IN |
denotes |
to |
T20481 |
308-310 |
VB |
denotes |
be |
T20480 |
302-307 |
MD |
denotes |
could |
T20479 |
311-320 |
VBN |
denotes |
converted |
T20478 |
297-301 |
WDT |
denotes |
that |
T20477 |
282-286 |
NN |
denotes |
MSUD |
T20476 |
269-281 |
JJ |
denotes |
intermediate |
T20475 |
287-296 |
NN |
denotes |
phenotype |
T20474 |
266-268 |
DT |
denotes |
an |
T20473 |
261-265 |
IN |
denotes |
with |
T20472 |
256-260 |
NNS |
denotes |
mice |
T20471 |
249-255 |
VB |
denotes |
create |
T20470 |
246-248 |
TO |
denotes |
to |
T20469 |
233-241 |
NN |
denotes |
approach |
T20468 |
229-232 |
PRP$ |
denotes |
our |
T20467 |
222-228 |
IN |
denotes |
behind |
T20466 |
242-245 |
VBD |
denotes |
was |
T20465 |
213-221 |
NN |
denotes |
strategy |
T20464 |
209-212 |
DT |
denotes |
The |
T20463 |
208-417 |
sentence |
denotes |
The strategy behind our approach was to create mice with an intermediate MSUD phenotype that could be converted to the classic phenotype at the investigator's discretion by turning off the rescuing transgene. |
T20462 |
207-208 |
. |
denotes |
. |
T20461 |
206-207 |
-RRB- |
denotes |
] |
T20460 |
204-206 |
CD |
denotes |
34 |
T20459 |
203-204 |
-LRB- |
denotes |
[ |
T20448 |
140-143 |
VBZ |
denotes |
has |
T20447 |
149-153 |
VBN |
denotes |
used |
T20446 |
135-139 |
WDT |
denotes |
that |
T20445 |
121-127 |
NN |
denotes |
switch |
T20444 |
116-120 |
NN |
denotes |
gene |
T20443 |
106-115 |
VBN |
denotes |
regulated |
T20442 |
93-105 |
NN |
denotes |
tetracycline |
T20441 |
128-134 |
NN |
denotes |
system |
T20440 |
89-92 |
DT |
denotes |
the |
T20439 |
86-88 |
IN |
denotes |
on |
T20438 |
76-79 |
VBD |
denotes |
was |
T20437 |
65-69 |
NN |
denotes |
MSUD |
T20436 |
52-64 |
JJ |
denotes |
intermediate |
T20435 |
70-75 |
NN |
denotes |
model |
T20434 |
48-51 |
DT |
denotes |
the |
T20433 |
41-47 |
VB |
denotes |
create |
T20432 |
38-40 |
TO |
denotes |
to |
T20431 |
29-32 |
VBD |
denotes |
was |
T20430 |
33-37 |
VBN |
denotes |
used |
T20429 |
24-28 |
WDT |
denotes |
that |
T20428 |
80-85 |
VBN |
denotes |
based |
T20427 |
4-14 |
JJ |
denotes |
transgenic |
T20426 |
15-23 |
NN |
denotes |
approach |
T20425 |
0-3 |
DT |
denotes |
The |
T20458 |
195-202 |
NN |
denotes |
example |
T20457 |
191-194 |
IN |
denotes |
for |
T20456 |
189-191 |
, |
denotes |
, |
T20455 |
182-189 |
NNS |
denotes |
studies |
T20454 |
176-181 |
JJ |
denotes |
other |
T20453 |
173-175 |
IN |
denotes |
in |
T20452 |
165-172 |
NN |
denotes |
success |
T20451 |
159-164 |
JJ |
denotes |
great |
T20450 |
154-158 |
IN |
denotes |
with |
T20449 |
144-148 |
VBN |
denotes |
been |
R5517 |
T20425 |
T20426 |
det |
The,approach |
R5518 |
T20426 |
T20428 |
nsubjpass |
approach,based |
R5519 |
T20427 |
T20426 |
amod |
transgenic,approach |
R5520 |
T20429 |
T20430 |
dep |
that,used |
R5521 |
T20430 |
T20426 |
relcl |
used,approach |
R5522 |
T20431 |
T20430 |
auxpass |
was,used |
R5523 |
T20432 |
T20433 |
aux |
to,create |
R5524 |
T20433 |
T20430 |
advcl |
create,used |
R5525 |
T20434 |
T20435 |
det |
the,model |
R5526 |
T20435 |
T20433 |
dobj |
model,create |
R5527 |
T20436 |
T20435 |
amod |
intermediate,model |
R5528 |
T20437 |
T20435 |
compound |
MSUD,model |
R5529 |
T20438 |
T20428 |
auxpass |
was,based |
R5530 |
T20439 |
T20428 |
prep |
on,based |
R5531 |
T20440 |
T20441 |
det |
the,system |
R5532 |
T20441 |
T20439 |
pobj |
system,on |
R5533 |
T20442 |
T20443 |
npadvmod |
tetracycline,regulated |
R5534 |
T20443 |
T20441 |
amod |
regulated,system |
R5535 |
T20444 |
T20445 |
compound |
gene,switch |
R5536 |
T20445 |
T20441 |
compound |
switch,system |
R5537 |
T20446 |
T20447 |
dep |
that,used |
R5538 |
T20447 |
T20441 |
relcl |
used,system |
R5539 |
T20448 |
T20447 |
aux |
has,used |
R5540 |
T20449 |
T20447 |
auxpass |
been,used |
R5541 |
T20450 |
T20447 |
prep |
with,used |
R5542 |
T20451 |
T20452 |
amod |
great,success |
R5543 |
T20452 |
T20450 |
pobj |
success,with |
R5544 |
T20453 |
T20447 |
prep |
in,used |
R5545 |
T20454 |
T20455 |
amod |
other,studies |
R5546 |
T20455 |
T20453 |
pobj |
studies,in |
R5547 |
T20456 |
T20447 |
punct |
", ",used |
R5548 |
T20457 |
T20447 |
prep |
for,used |
R5549 |
T20458 |
T20457 |
pobj |
example,for |
R5550 |
T20459 |
T20460 |
punct |
[,34 |
R5551 |
T20460 |
T20428 |
parataxis |
34,based |
R5552 |
T20461 |
T20460 |
punct |
],34 |
R5553 |
T20462 |
T20428 |
punct |
.,based |
R5554 |
T20464 |
T20465 |
det |
The,strategy |
R5555 |
T20465 |
T20466 |
nsubj |
strategy,was |
R5556 |
T20467 |
T20465 |
prep |
behind,strategy |
R5557 |
T20468 |
T20469 |
poss |
our,approach |
R5558 |
T20469 |
T20467 |
pobj |
approach,behind |
R5559 |
T20470 |
T20471 |
aux |
to,create |
R5560 |
T20471 |
T20466 |
xcomp |
create,was |
R5561 |
T20472 |
T20471 |
dobj |
mice,create |
R5562 |
T20473 |
T20472 |
prep |
with,mice |
R5563 |
T20474 |
T20475 |
det |
an,phenotype |
R5564 |
T20475 |
T20473 |
pobj |
phenotype,with |
R5565 |
T20476 |
T20475 |
amod |
intermediate,phenotype |
R5566 |
T20477 |
T20475 |
compound |
MSUD,phenotype |
R5567 |
T20478 |
T20479 |
dep |
that,converted |
R5568 |
T20479 |
T20475 |
relcl |
converted,phenotype |
R5569 |
T20480 |
T20479 |
aux |
could,converted |
R5570 |
T20481 |
T20479 |
auxpass |
be,converted |
R5571 |
T20482 |
T20479 |
prep |
to,converted |
R5572 |
T20483 |
T20484 |
det |
the,phenotype |
R5573 |
T20484 |
T20482 |
pobj |
phenotype,to |
R5574 |
T20485 |
T20484 |
amod |
classic,phenotype |
R5575 |
T20486 |
T20479 |
prep |
at,converted |
R5576 |
T20487 |
T20488 |
det |
the,investigator |
R5577 |
T20488 |
T20489 |
poss |
investigator,discretion |
R5578 |
T20489 |
T20486 |
pobj |
discretion,at |
R5579 |
T20490 |
T20488 |
case |
's,investigator |
R5580 |
T20491 |
T20479 |
prep |
by,converted |
R5581 |
T20492 |
T20491 |
pcomp |
turning,by |
R5582 |
T20493 |
T20492 |
prt |
off,turning |
R5583 |
T20494 |
T20495 |
det |
the,transgene |
R5584 |
T20495 |
T20492 |
dobj |
transgene,turning |
R5585 |
T20496 |
T20495 |
amod |
rescuing,transgene |
R5586 |
T20497 |
T20466 |
punct |
.,was |
R5587 |
T20499 |
T20500 |
advmod |
However,were |
R5588 |
T20501 |
T20500 |
punct |
", ",were |
R5589 |
T20502 |
T20500 |
nsubj |
we,were |
R5590 |
T20503 |
T20500 |
acomp |
unable,were |
R5591 |
T20504 |
T20505 |
aux |
to,turn |
R5592 |
T20505 |
T20503 |
xcomp |
turn,unable |
R5593 |
T20506 |
T20505 |
advmod |
consistently,turn |
R5594 |
T20507 |
T20505 |
prt |
off,turn |
R5595 |
T20508 |
T20509 |
det |
the,cassette |
R5596 |
T20509 |
T20505 |
dobj |
cassette,turn |
R5597 |
T20510 |
T20509 |
amod |
human,cassette |
R5598 |
T20511 |
T20509 |
compound |
E2,cassette |
R5599 |
T20512 |
T20509 |
compound |
expression,cassette |
R5600 |
T20513 |
T20505 |
prep |
by,turn |
R5601 |
T20514 |
T20515 |
det |
the,analogue |
R5602 |
T20515 |
T20513 |
pobj |
analogue,by |
R5603 |
T20516 |
T20515 |
amod |
potent,analogue |
R5604 |
T20517 |
T20515 |
compound |
tetracycline,analogue |
R5605 |
T20518 |
T20515 |
punct |
", ",analogue |
R5606 |
T20519 |
T20515 |
appos |
doxycycline,analogue |
R5607 |
T20520 |
T20505 |
prep |
in,turn |
R5608 |
T20521 |
T20520 |
pobj |
either,in |
R5609 |
T20522 |
T20521 |
prep |
of,either |
R5610 |
T20523 |
T20524 |
det |
the,lines |
R5611 |
T20524 |
T20522 |
pobj |
lines,of |
R5612 |
T20525 |
T20524 |
nummod |
two,lines |
R5613 |
T20526 |
T20524 |
amod |
transgenic,lines |
R5614 |
T20527 |
T20524 |
acl |
tested,lines |
R5615 |
T20528 |
T20529 |
punct |
(,shown |
R5616 |
T20529 |
T20500 |
parataxis |
shown,were |
R5617 |
T20530 |
T20529 |
nsubj |
data,shown |
R5618 |
T20531 |
T20529 |
neg |
not,shown |
R5619 |
T20532 |
T20529 |
punct |
),shown |
R5620 |
T20533 |
T20500 |
punct |
.,were |
R5621 |
T20535 |
T20536 |
det |
The,reason |
R5622 |
T20536 |
T20537 |
nsubj |
reason,is |
R5623 |
T20538 |
T20539 |
det |
these,lines |
R5624 |
T20539 |
T20541 |
nsubj |
lines,were |
R5625 |
T20540 |
T20539 |
nummod |
two,lines |
R5626 |
T20541 |
T20536 |
advcl |
were,reason |
R5627 |
T20542 |
T20541 |
acomp |
unresponsive,were |
R5628 |
T20543 |
T20542 |
prep |
to,unresponsive |
R5629 |
T20544 |
T20543 |
pobj |
doxycycline,to |
R5630 |
T20545 |
T20537 |
acomp |
unknown,is |
R5631 |
T20546 |
T20537 |
punct |
.,is |
R5632 |
T20548 |
T20549 |
nsubj |
It,is |
R5633 |
T20550 |
T20549 |
acomp |
conceivable,is |
R5634 |
T20551 |
T20552 |
mark |
that,was |
R5635 |
T20552 |
T20549 |
ccomp |
was,is |
R5636 |
T20553 |
T20554 |
det |
the,site |
R5637 |
T20554 |
T20552 |
nsubj |
site,was |
R5638 |
T20555 |
T20554 |
compound |
integration,site |
R5639 |
T20556 |
T20554 |
prep |
of,site |
R5640 |
T20557 |
T20558 |
det |
the,transgenes |
R5641 |
T20558 |
T20556 |
pobj |
transgenes,of |
R5642 |
T20559 |
T20552 |
neg |
not,was |
R5643 |
T20560 |
T20552 |
acomp |
permissive,was |
R5644 |
T20561 |
T20560 |
prep |
for,permissive |
R5645 |
T20562 |
T20563 |
amod |
regulated,expression |
R5646 |
T20563 |
T20561 |
pobj |
expression,for |
R5647 |
T20564 |
T20549 |
punct |
.,is |
R5648 |
T20566 |
T20567 |
nsubjpass |
Screening,needed |
R5649 |
T20568 |
T20566 |
prep |
of,Screening |
R5650 |
T20569 |
T20570 |
amod |
additional,lines |
R5651 |
T20570 |
T20568 |
pobj |
lines,of |
R5652 |
T20571 |
T20567 |
aux |
may,needed |
R5653 |
T20572 |
T20567 |
auxpass |
be,needed |
R5654 |
T20573 |
T20574 |
aux |
to,find |
R5655 |
T20574 |
T20567 |
advcl |
find,needed |
R5656 |
T20575 |
T20574 |
dobj |
lines,find |
R5657 |
T20576 |
T20577 |
dep |
that,allow |
R5658 |
T20577 |
T20575 |
relcl |
allow,lines |
R5659 |
T20578 |
T20577 |
prep |
for,allow |
R5660 |
T20579 |
T20578 |
pobj |
survival,for |
R5661 |
T20580 |
T20577 |
cc |
and,allow |
R5662 |
T20581 |
T20582 |
aux |
can,regulated |
R5663 |
T20582 |
T20577 |
conj |
regulated,allow |
R5664 |
T20583 |
T20582 |
advmod |
also,regulated |
R5665 |
T20584 |
T20582 |
auxpass |
be,regulated |
R5666 |
T20585 |
T20567 |
punct |
.,needed |