Id |
Subject |
Object |
Predicate |
Lexical cue |
T23927 |
1263-1265 |
VB |
denotes |
be |
T23926 |
1260-1262 |
TO |
denotes |
to |
T23925 |
1255-1259 |
NN |
denotes |
lane |
T23924 |
1250-1254 |
DT |
denotes |
each |
T23923 |
1247-1249 |
IN |
denotes |
in |
T23922 |
1240-1246 |
VBN |
denotes |
loaded |
T23921 |
1232-1239 |
NN |
denotes |
protein |
T23920 |
1229-1231 |
IN |
denotes |
of |
T23919 |
1266-1274 |
VBN |
denotes |
compared |
T23918 |
1222-1228 |
NN |
denotes |
amount |
T23917 |
1216-1221 |
VB |
denotes |
allow |
T23916 |
1213-1215 |
TO |
denotes |
to |
T23915 |
1198-1203 |
NN |
denotes |
actin |
T23914 |
1204-1212 |
NN |
denotes |
antibody |
T23913 |
1195-1197 |
DT |
denotes |
an |
T23912 |
1190-1194 |
IN |
denotes |
with |
T23911 |
1175-1179 |
VBD |
denotes |
were |
T23910 |
1180-1189 |
VBN |
denotes |
re-probed |
T23909 |
1169-1174 |
NNS |
denotes |
blots |
T23908 |
1165-1168 |
DT |
denotes |
All |
T23907 |
1164-1275 |
sentence |
denotes |
All blots were re-probed with an actin antibody to allow amount of protein loaded in each lane to be compared. |
T23906 |
1163-1164 |
. |
denotes |
. |
T23905 |
1156-1163 |
NNS |
denotes |
tissues |
T23904 |
1150-1155 |
DT |
denotes |
those |
T23903 |
1147-1149 |
IN |
denotes |
in |
T23902 |
1144-1146 |
NN |
denotes |
E2 |
T23901 |
1136-1143 |
VBN |
denotes |
derived |
T23900 |
1126-1135 |
NN |
denotes |
transgene |
T23899 |
1123-1125 |
IN |
denotes |
of |
T23898 |
1115-1122 |
NNS |
denotes |
amounts |
T23897 |
1104-1114 |
JJ |
denotes |
negligible |
T23896 |
1093-1094 |
-RRB- |
denotes |
) |
T23895 |
1092-1093 |
NN |
denotes |
E |
T23894 |
1091-1092 |
-LRB- |
denotes |
( |
T23893 |
1084-1090 |
NN |
denotes |
muscle |
T23892 |
1080-1083 |
CC |
denotes |
and |
T23891 |
1078-1080 |
, |
denotes |
, |
T23890 |
1077-1078 |
-RRB- |
denotes |
) |
T23889 |
1076-1077 |
NN |
denotes |
D |
T23888 |
1075-1076 |
-LRB- |
denotes |
( |
T23887 |
1068-1074 |
NN |
denotes |
kidney |
T23886 |
1066-1068 |
, |
denotes |
, |
T23885 |
1065-1066 |
-RRB- |
denotes |
) |
T23884 |
1064-1065 |
NN |
denotes |
C |
T23883 |
1063-1064 |
-LRB- |
denotes |
( |
T23882 |
1057-1062 |
NN |
denotes |
brain |
T23881 |
1054-1056 |
IN |
denotes |
of |
T23880 |
1095-1103 |
VBD |
denotes |
revealed |
T23879 |
1045-1053 |
NN |
denotes |
analysis |
T23878 |
1040-1044 |
NN |
denotes |
blot |
T23877 |
1032-1039 |
NNP |
denotes |
Western |
T23876 |
1031-1164 |
sentence |
denotes |
Western blot analysis of brain (C), kidney (D), and muscle (E) revealed negligible amounts of transgene derived E2 in those tissues. |
T23875 |
1030-1031 |
. |
denotes |
. |
T23874 |
1022-1030 |
NNS |
denotes |
controls |
T23873 |
1019-1021 |
IN |
denotes |
in |
T23872 |
1015-1018 |
RB |
denotes |
not |
T23871 |
1011-1014 |
CC |
denotes |
but |
T23870 |
1006-1010 |
NNS |
denotes |
mice |
T23869 |
995-1005 |
JJ |
denotes |
transgenic |
T23868 |
992-994 |
IN |
denotes |
in |
T23867 |
983-988 |
JJ |
denotes |
human |
T23866 |
981-983 |
, |
denotes |
, |
T23865 |
975-981 |
VBN |
denotes |
tagged |
T23864 |
970-971 |
HYPH |
denotes |
- |
T23863 |
971-974 |
NN |
denotes |
myc |
T23862 |
969-970 |
NN |
denotes |
c |
T23861 |
967-969 |
, |
denotes |
, |
T23860 |
960-967 |
VBN |
denotes |
derived |
T23859 |
950-959 |
NN |
denotes |
transgene |
T23858 |
989-991 |
NN |
denotes |
E2 |
T23857 |
946-949 |
DT |
denotes |
the |
T23856 |
943-945 |
IN |
denotes |
of |
T23855 |
934-942 |
NN |
denotes |
presence |
T23854 |
930-933 |
DT |
denotes |
the |
T23853 |
907-910 |
NN |
denotes |
tag |
T23852 |
902-903 |
HYPH |
denotes |
- |
T23851 |
903-906 |
NN |
denotes |
myc |
T23850 |
901-902 |
NN |
denotes |
c |
T23849 |
911-919 |
NN |
denotes |
antibody |
T23848 |
899-900 |
DT |
denotes |
a |
T23847 |
894-898 |
IN |
denotes |
with |
T23846 |
920-929 |
VBD |
denotes |
confirmed |
T23845 |
885-886 |
HYPH |
denotes |
- |
T23844 |
886-893 |
NN |
denotes |
probing |
T23843 |
883-885 |
NN |
denotes |
Re |
T23842 |
882-1031 |
sentence |
denotes |
Re-probing with a c-myc tag antibody confirmed the presence of the transgene derived, c-myc tagged, human E2 in transgenic mice but not in controls. |
T23841 |
881-882 |
. |
denotes |
. |
T23840 |
874-881 |
NNS |
denotes |
animals |
T23839 |
866-873 |
NN |
denotes |
control |
T23838 |
863-865 |
IN |
denotes |
in |
T23837 |
861-862 |
-RRB- |
denotes |
) |
T23836 |
856-858 |
CD |
denotes |
47 |
T23835 |
855-856 |
SYM |
denotes |
~ |
T23834 |
854-855 |
SYM |
denotes |
= |
T23833 |
852-854 |
NN |
denotes |
MW |
T23832 |
859-861 |
NN |
denotes |
Kd |
T23831 |
851-852 |
-LRB- |
denotes |
( |
T23830 |
848-850 |
NN |
denotes |
E2 |
T23829 |
842-847 |
NN |
denotes |
mouse |
T23828 |
839-841 |
IN |
denotes |
of |
T23827 |
832-838 |
NN |
denotes |
amount |
T23826 |
828-831 |
DT |
denotes |
the |
T23825 |
825-827 |
IN |
denotes |
to |
T23824 |
817-824 |
JJ |
denotes |
similar |
T23823 |
806-812 |
NN |
denotes |
amount |
T23822 |
802-805 |
DT |
denotes |
the |
T23821 |
794-801 |
NNS |
denotes |
animals |
T23820 |
790-793 |
DT |
denotes |
the |
T23819 |
787-789 |
IN |
denotes |
of |
T23818 |
782-786 |
JJ |
denotes |
many |
T23817 |
813-816 |
VBD |
denotes |
was |
T23816 |
779-781 |
IN |
denotes |
in |
T23815 |
775-778 |
CC |
denotes |
but |
T23814 |
766-774 |
JJ |
denotes |
variable |
T23813 |
760-761 |
NN |
denotes |
A |
T23812 |
756-759 |
CD |
denotes |
525 |
T23811 |
752-755 |
CC |
denotes |
and |
T23810 |
750-751 |
NN |
denotes |
A |
T23809 |
744-749 |
NNS |
denotes |
Lines |
T23808 |
739-743 |
IN |
denotes |
from |
T23807 |
734-738 |
NNS |
denotes |
mice |
T23806 |
731-733 |
IN |
denotes |
in |
T23805 |
729-730 |
-RRB- |
denotes |
) |
T23804 |
727-729 |
NN |
denotes |
Kd |
T23803 |
724-726 |
CD |
denotes |
54 |
T23802 |
723-724 |
SYM |
denotes |
~ |
T23801 |
710-719 |
VBN |
denotes |
predicted |
T23800 |
720-722 |
NN |
denotes |
MW |
T23799 |
709-710 |
-LRB- |
denotes |
( |
T23798 |
698-700 |
NN |
denotes |
E2 |
T23797 |
701-708 |
NN |
denotes |
protein |
T23796 |
692-697 |
JJ |
denotes |
human |
T23795 |
689-691 |
IN |
denotes |
of |
T23794 |
682-688 |
NN |
denotes |
amount |
T23793 |
678-681 |
DT |
denotes |
the |
T23792 |
762-765 |
VBD |
denotes |
was |
T23791 |
673-677 |
IN |
denotes |
that |
T23790 |
668-672 |
VB |
denotes |
Note |
T23789 |
667-882 |
sentence |
denotes |
Note that the amount of human E2 protein (predicted MW ~54 Kd) in mice from Lines A and 525 A was variable but in many of the animals the amount was similar to the amount of mouse E2 (MW=~47 Kd) in control animals. |
T23788 |
666-667 |
. |
denotes |
. |
T23787 |
657-661 |
NN |
denotes |
MSUD |
T23786 |
644-656 |
JJ |
denotes |
intermediate |
T23785 |
640-643 |
CC |
denotes |
and |
T23784 |
662-666 |
NNS |
denotes |
mice |
T23783 |
632-639 |
NN |
denotes |
control |
T23782 |
629-631 |
IN |
denotes |
of |
T23781 |
623-628 |
NN |
denotes |
liver |
T23780 |
620-622 |
IN |
denotes |
in |
T23779 |
612-619 |
NN |
denotes |
protein |
T23778 |
609-611 |
NN |
denotes |
E2 |
T23777 |
606-608 |
IN |
denotes |
of |
T23776 |
592-596 |
NN |
denotes |
blot |
T23775 |
584-591 |
NNP |
denotes |
Western |
T23774 |
582-584 |
, |
denotes |
, |
T23773 |
597-605 |
NN |
denotes |
analysis |
T23772 |
581-582 |
LS |
denotes |
B |
T23771 |
580-667 |
sentence |
denotes |
B, Western blot analysis of E2 protein in liver of control and intermediate MSUD mice. |
T23770 |
579-580 |
. |
denotes |
. |
T23769 |
575-579 |
NNS |
denotes |
mice |
T23768 |
564-574 |
JJ |
denotes |
transgenic |
T23767 |
561-563 |
IN |
denotes |
of |
T23766 |
555-560 |
NNS |
denotes |
lines |
T23765 |
547-554 |
JJ |
denotes |
several |
T23764 |
540-546 |
VB |
denotes |
create |
T23763 |
537-539 |
TO |
denotes |
to |
T23762 |
528-531 |
VBD |
denotes |
was |
T23761 |
532-536 |
VBN |
denotes |
used |
T23760 |
518-527 |
NN |
denotes |
construct |
T23759 |
513-517 |
DT |
denotes |
This |
T23758 |
512-580 |
sentence |
denotes |
This construct was used to create several lines of transgenic mice. |
T23757 |
511-512 |
. |
denotes |
. |
T23756 |
487-502 |
NN |
denotes |
polyadenylation |
T23755 |
503-511 |
NN |
denotes |
sequence |
T23754 |
479-486 |
VBN |
denotes |
derived |
T23753 |
474-478 |
NN |
denotes |
SV40 |
T23752 |
470-473 |
CC |
denotes |
and |
T23751 |
468-470 |
, |
denotes |
, |
T23750 |
457-464 |
NN |
denotes |
epitope |
T23749 |
452-453 |
HYPH |
denotes |
- |
T23748 |
453-456 |
NN |
denotes |
myc |
T23747 |
451-452 |
NN |
denotes |
c |
T23746 |
465-468 |
NN |
denotes |
tag |
T23745 |
449-450 |
DT |
denotes |
a |
T23744 |
447-449 |
, |
denotes |
, |
T23743 |
433-440 |
NN |
denotes |
alanine |
T23742 |
441-447 |
NN |
denotes |
spacer |
T23741 |
430-432 |
DT |
denotes |
an |
T23740 |
428-430 |
, |
denotes |
, |
T23739 |
421-423 |
NN |
denotes |
E2 |
T23738 |
415-420 |
JJ |
denotes |
human |
T23737 |
424-428 |
NN |
denotes |
cDNA |
T23736 |
411-414 |
DT |
denotes |
the |
T23735 |
409-411 |
, |
denotes |
, |
T23734 |
408-409 |
-RRB- |
denotes |
) |
T23733 |
399-403 |
JJ |
denotes |
thin |
T23732 |
404-408 |
NN |
denotes |
line |
T23731 |
398-399 |
-LRB- |
denotes |
( |
T23730 |
381-390 |
JJ |
denotes |
synthetic |
T23729 |
391-397 |
NN |
denotes |
intron |
T23728 |
379-380 |
DT |
denotes |
a |
T23727 |
377-379 |
, |
denotes |
, |
T23726 |
369-377 |
NN |
denotes |
promoter |
T23725 |
365-368 |
DT |
denotes |
the |
T23724 |
362-364 |
IN |
denotes |
of |
T23723 |
357-361 |
NN |
denotes |
part |
T23722 |
354-356 |
IN |
denotes |
as |
T23721 |
352-353 |
-RRB- |
denotes |
) |
T23720 |
349-352 |
NN |
denotes |
TRE |
T23719 |
348-349 |
-LRB- |
denotes |
( |
T23718 |
331-339 |
NN |
denotes |
response |
T23717 |
318-330 |
NN |
denotes |
tetracycline |
T23716 |
340-347 |
NN |
denotes |
element |
T23715 |
314-317 |
DT |
denotes |
the |
T23714 |
305-313 |
VBZ |
denotes |
contains |
T23713 |
291-292 |
HYPH |
denotes |
- |
T23712 |
292-294 |
NN |
denotes |
E2 |
T23711 |
288-291 |
NN |
denotes |
TRE |
T23710 |
295-304 |
NN |
denotes |
transgene |
T23709 |
284-287 |
DT |
denotes |
The |
T23708 |
283-512 |
sentence |
denotes |
The TRE-E2 transgene contains the tetracycline response element (TRE) as part of the promoter, a synthetic intron (thin line), the human E2 cDNA, an alanine spacer, a c-myc epitope tag, and SV40 derived polyadenylation sequence. |
T23707 |
282-283 |
. |
denotes |
. |
T23706 |
270-273 |
NN |
denotes |
LAP |
T23705 |
261-269 |
JJ |
denotes |
specific |
T23704 |
255-260 |
NN |
denotes |
liver |
T23703 |
274-282 |
NN |
denotes |
promoter |
T23702 |
251-254 |
DT |
denotes |
the |
T23701 |
246-250 |
IN |
denotes |
from |
T23700 |
244-245 |
-RRB- |
denotes |
) |
T23699 |
241-244 |
NN |
denotes |
tTA |
T23698 |
240-241 |
-LRB- |
denotes |
( |
T23697 |
213-214 |
HYPH |
denotes |
- |
T23696 |
214-224 |
VBN |
denotes |
controlled |
T23695 |
201-213 |
NN |
denotes |
tetracycline |
T23694 |
225-239 |
NN |
denotes |
transactivator |
T23693 |
197-200 |
DT |
denotes |
the |
T23692 |
189-196 |
VBP |
denotes |
express |
T23691 |
184-188 |
NNS |
denotes |
mice |
T23690 |
178-183 |
DT |
denotes |
These |
T23689 |
177-283 |
sentence |
denotes |
These mice express the tetracycline-controlled transactivator (tTA) from the liver specific LAP promoter. |
T23688 |
176-177 |
. |
denotes |
. |
T23687 |
175-176 |
-RRB- |
denotes |
] |
T23686 |
173-175 |
CD |
denotes |
34 |
T23685 |
172-173 |
-LRB- |
denotes |
[ |
T23684 |
151-161 |
RB |
denotes |
previously |
T23683 |
146-150 |
VBN |
denotes |
been |
T23682 |
141-145 |
VBP |
denotes |
have |
T23681 |
162-171 |
VBN |
denotes |
described |
T23680 |
125-135 |
JJ |
denotes |
transgenic |
T23679 |
136-140 |
NNS |
denotes |
mice |
T23678 |
120-121 |
HYPH |
denotes |
- |
T23677 |
121-124 |
NN |
denotes |
tTA |
T23676 |
117-120 |
NN |
denotes |
LAP |
T23675 |
116-177 |
sentence |
denotes |
LAP-tTA transgenic mice have been previously described [34]. |
T23674 |
115-116 |
. |
denotes |
. |
T23673 |
113-115 |
NN |
denotes |
E2 |
T23672 |
107-112 |
JJ |
denotes |
human |
T23671 |
99-106 |
VBP |
denotes |
express |
T23670 |
94-98 |
WDT |
denotes |
that |
T23669 |
89-93 |
NNS |
denotes |
mice |
T23668 |
81-88 |
VB |
denotes |
produce |
T23667 |
78-80 |
TO |
denotes |
to |
T23666 |
73-77 |
VBN |
denotes |
used |
T23665 |
53-63 |
JJ |
denotes |
Transgenic |
T23664 |
51-53 |
, |
denotes |
, |
T23663 |
64-72 |
NN |
denotes |
strategy |
T23662 |
50-51 |
LS |
denotes |
A |
T23661 |
49-116 |
sentence |
denotes |
A, Transgenic strategy used to produce mice that express human E2. |
T23660 |
48-49 |
. |
denotes |
. |
T23659 |
32-48 |
NN |
denotes |
characterization |
T23658 |
28-31 |
CC |
denotes |
and |
T23657 |
11-16 |
NN |
denotes |
mouse |
T23656 |
17-27 |
NN |
denotes |
production |
T23655 |
0-10 |
JJ |
denotes |
Transgenic |
T23928 |
1274-1275 |
. |
denotes |
. |
R6739 |
T23655 |
T23656 |
amod |
Transgenic,production |
R6740 |
T23657 |
T23656 |
compound |
mouse,production |
R6741 |
T23658 |
T23656 |
cc |
and,production |
R6742 |
T23659 |
T23656 |
conj |
characterization,production |
R6743 |
T23660 |
T23656 |
punct |
.,production |
R6744 |
T23662 |
T23663 |
meta |
A,strategy |
R6745 |
T23664 |
T23663 |
punct |
", ",strategy |
R6746 |
T23665 |
T23663 |
amod |
Transgenic,strategy |
R6747 |
T23666 |
T23663 |
acl |
used,strategy |
R6748 |
T23667 |
T23668 |
aux |
to,produce |
R6749 |
T23668 |
T23666 |
advcl |
produce,used |
R6750 |
T23669 |
T23668 |
dobj |
mice,produce |
R6751 |
T23670 |
T23671 |
dep |
that,express |
R6752 |
T23671 |
T23669 |
relcl |
express,mice |
R6753 |
T23672 |
T23673 |
amod |
human,E2 |
R6754 |
T23673 |
T23671 |
dobj |
E2,express |
R6755 |
T23674 |
T23663 |
punct |
.,strategy |
R6756 |
T23676 |
T23677 |
nmod |
LAP,tTA |
R6757 |
T23677 |
T23679 |
nmod |
tTA,mice |
R6758 |
T23678 |
T23677 |
punct |
-,tTA |
R6759 |
T23679 |
T23681 |
nsubjpass |
mice,described |
R6760 |
T23680 |
T23679 |
amod |
transgenic,mice |
R6761 |
T23682 |
T23681 |
aux |
have,described |
R6762 |
T23683 |
T23681 |
auxpass |
been,described |
R6763 |
T23684 |
T23681 |
advmod |
previously,described |
R6764 |
T23685 |
T23686 |
punct |
[,34 |
R6765 |
T23686 |
T23681 |
parataxis |
34,described |
R6766 |
T23687 |
T23686 |
punct |
],34 |
R6767 |
T23688 |
T23681 |
punct |
.,described |
R6768 |
T23690 |
T23691 |
det |
These,mice |
R6769 |
T23691 |
T23692 |
nsubj |
mice,express |
R6770 |
T23693 |
T23694 |
det |
the,transactivator |
R6771 |
T23694 |
T23692 |
dobj |
transactivator,express |
R6772 |
T23695 |
T23696 |
npadvmod |
tetracycline,controlled |
R6773 |
T23696 |
T23694 |
amod |
controlled,transactivator |
R6774 |
T23697 |
T23696 |
punct |
-,controlled |
R6775 |
T23698 |
T23694 |
punct |
(,transactivator |
R6776 |
T23699 |
T23694 |
appos |
tTA,transactivator |
R6777 |
T23700 |
T23694 |
punct |
),transactivator |
R6778 |
T23701 |
T23694 |
prep |
from,transactivator |
R6779 |
T23702 |
T23703 |
det |
the,promoter |
R6780 |
T23703 |
T23701 |
pobj |
promoter,from |
R6781 |
T23704 |
T23705 |
npadvmod |
liver,specific |
R6782 |
T23705 |
T23703 |
amod |
specific,promoter |
R6783 |
T23706 |
T23703 |
compound |
LAP,promoter |
R6784 |
T23707 |
T23692 |
punct |
.,express |
R6785 |
T23709 |
T23710 |
det |
The,transgene |
R6786 |
T23710 |
T23714 |
nsubj |
transgene,contains |
R6787 |
T23711 |
T23712 |
compound |
TRE,E2 |
R6788 |
T23712 |
T23710 |
compound |
E2,transgene |
R6789 |
T23713 |
T23712 |
punct |
-,E2 |
R6790 |
T23715 |
T23716 |
det |
the,element |
R6791 |
T23716 |
T23714 |
dobj |
element,contains |
R6792 |
T23717 |
T23716 |
compound |
tetracycline,element |
R6793 |
T23718 |
T23716 |
compound |
response,element |
R6794 |
T23719 |
T23716 |
punct |
(,element |
R6795 |
T23720 |
T23716 |
appos |
TRE,element |
R6796 |
T23721 |
T23716 |
punct |
),element |
R6797 |
T23722 |
T23716 |
prep |
as,element |
R6798 |
T23723 |
T23722 |
pobj |
part,as |
R6799 |
T23724 |
T23723 |
prep |
of,part |
R6800 |
T23725 |
T23726 |
det |
the,promoter |
R6801 |
T23726 |
T23724 |
pobj |
promoter,of |
R6802 |
T23727 |
T23716 |
punct |
", ",element |
R6803 |
T23728 |
T23729 |
det |
a,intron |
R6804 |
T23729 |
T23716 |
conj |
intron,element |
R6805 |
T23730 |
T23729 |
amod |
synthetic,intron |
R6806 |
T23731 |
T23732 |
punct |
(,line |
R6807 |
T23732 |
T23729 |
parataxis |
line,intron |
R6808 |
T23733 |
T23732 |
amod |
thin,line |
R6809 |
T23734 |
T23732 |
punct |
),line |
R6810 |
T23735 |
T23729 |
punct |
", ",intron |
R6811 |
T23736 |
T23737 |
det |
the,cDNA |
R6812 |
T23737 |
T23729 |
conj |
cDNA,intron |
R6813 |
T23738 |
T23737 |
amod |
human,cDNA |
R6814 |
T23739 |
T23737 |
compound |
E2,cDNA |
R6815 |
T23740 |
T23737 |
punct |
", ",cDNA |
R6816 |
T23741 |
T23742 |
det |
an,spacer |
R6817 |
T23742 |
T23737 |
conj |
spacer,cDNA |
R6818 |
T23743 |
T23742 |
compound |
alanine,spacer |
R6819 |
T23744 |
T23742 |
punct |
", ",spacer |
R6820 |
T23745 |
T23746 |
det |
a,tag |
R6821 |
T23746 |
T23742 |
conj |
tag,spacer |
R6822 |
T23747 |
T23748 |
compound |
c,myc |
R6823 |
T23748 |
T23746 |
compound |
myc,tag |
R6824 |
T23749 |
T23748 |
punct |
-,myc |
R6825 |
T23750 |
T23746 |
compound |
epitope,tag |
R6826 |
T23751 |
T23746 |
punct |
", ",tag |
R6827 |
T23752 |
T23746 |
cc |
and,tag |
R6828 |
T23753 |
T23754 |
npadvmod |
SV40,derived |
R6829 |
T23754 |
T23755 |
amod |
derived,sequence |
R6830 |
T23755 |
T23746 |
conj |
sequence,tag |
R6831 |
T23756 |
T23755 |
compound |
polyadenylation,sequence |
R6832 |
T23757 |
T23714 |
punct |
.,contains |
R6833 |
T23759 |
T23760 |
det |
This,construct |
R6834 |
T23760 |
T23761 |
nsubjpass |
construct,used |
R6835 |
T23762 |
T23761 |
auxpass |
was,used |
R6836 |
T23763 |
T23764 |
aux |
to,create |
R6837 |
T23764 |
T23761 |
advcl |
create,used |
R6838 |
T23765 |
T23766 |
amod |
several,lines |
R6839 |
T23766 |
T23764 |
dobj |
lines,create |
R6840 |
T23767 |
T23766 |
prep |
of,lines |
R6841 |
T23768 |
T23769 |
amod |
transgenic,mice |
R6842 |
T23769 |
T23767 |
pobj |
mice,of |
R6843 |
T23770 |
T23761 |
punct |
.,used |
R6844 |
T23772 |
T23773 |
meta |
B,analysis |
R6845 |
T23774 |
T23773 |
punct |
", ",analysis |
R6846 |
T23775 |
T23776 |
compound |
Western,blot |
R6847 |
T23776 |
T23773 |
compound |
blot,analysis |
R6848 |
T23777 |
T23773 |
prep |
of,analysis |
R6849 |
T23778 |
T23779 |
compound |
E2,protein |
R6850 |
T23779 |
T23777 |
pobj |
protein,of |
R6851 |
T23780 |
T23779 |
prep |
in,protein |
R6852 |
T23781 |
T23780 |
pobj |
liver,in |
R6853 |
T23782 |
T23781 |
prep |
of,liver |
R6854 |
T23783 |
T23784 |
nmod |
control,mice |
R6855 |
T23784 |
T23782 |
pobj |
mice,of |
R6856 |
T23785 |
T23783 |
cc |
and,control |
R6857 |
T23786 |
T23783 |
conj |
intermediate,control |
R6858 |
T23787 |
T23784 |
compound |
MSUD,mice |
R6859 |
T23788 |
T23773 |
punct |
.,analysis |
R6860 |
T23791 |
T23792 |
mark |
that,was |
R6861 |
T23792 |
T23790 |
ccomp |
was,Note |
R6862 |
T23793 |
T23794 |
det |
the,amount |
R6863 |
T23794 |
T23792 |
nsubj |
amount,was |
R6864 |
T23795 |
T23794 |
prep |
of,amount |
R6865 |
T23796 |
T23797 |
amod |
human,protein |
R6866 |
T23797 |
T23795 |
pobj |
protein,of |
R6867 |
T23798 |
T23797 |
compound |
E2,protein |
R6868 |
T23799 |
T23800 |
punct |
(,MW |
R6869 |
T23800 |
T23797 |
parataxis |
MW,protein |
R6870 |
T23801 |
T23800 |
amod |
predicted,MW |
R6871 |
T23802 |
T23803 |
punct |
~,54 |
R6872 |
T23803 |
T23804 |
nummod |
54,Kd |
R6873 |
T23804 |
T23800 |
appos |
Kd,MW |
R6874 |
T23805 |
T23800 |
punct |
),MW |
R6875 |
T23806 |
T23794 |
prep |
in,amount |
R6876 |
T23807 |
T23806 |
pobj |
mice,in |
R6877 |
T23808 |
T23807 |
prep |
from,mice |
R6878 |
T23809 |
T23810 |
compound |
Lines,A |
R6879 |
T23810 |
T23808 |
pobj |
A,from |
R6880 |
T23811 |
T23810 |
cc |
and,A |
R6881 |
T23812 |
T23813 |
nummod |
525,A |
R6882 |
T23813 |
T23810 |
conj |
A,A |
R6883 |
T23814 |
T23792 |
acomp |
variable,was |
R6884 |
T23815 |
T23792 |
cc |
but,was |
R6885 |
T23816 |
T23817 |
prep |
in,was |
R6886 |
T23817 |
T23792 |
conj |
was,was |
R6887 |
T23818 |
T23816 |
pobj |
many,in |
R6888 |
T23819 |
T23818 |
prep |
of,many |
R6889 |
T23820 |
T23821 |
det |
the,animals |
R6890 |
T23821 |
T23819 |
pobj |
animals,of |
R6891 |
T23822 |
T23823 |
det |
the,amount |
R6892 |
T23823 |
T23817 |
nsubj |
amount,was |
R6893 |
T23824 |
T23817 |
acomp |
similar,was |
R6894 |
T23825 |
T23824 |
prep |
to,similar |
R6895 |
T23826 |
T23827 |
det |
the,amount |
R6896 |
T23827 |
T23825 |
pobj |
amount,to |
R6897 |
T23828 |
T23827 |
prep |
of,amount |
R6898 |
T23829 |
T23830 |
compound |
mouse,E2 |
R6899 |
T23830 |
T23828 |
pobj |
E2,of |
R6900 |
T23831 |
T23832 |
punct |
(,Kd |
R6901 |
T23832 |
T23830 |
parataxis |
Kd,E2 |
R6902 |
T23833 |
T23832 |
nsubj |
MW,Kd |
R6903 |
T23834 |
T23832 |
punct |
=,Kd |
R6904 |
T23835 |
T23836 |
punct |
~,47 |
R6905 |
T23836 |
T23832 |
nummod |
47,Kd |
R6906 |
T23837 |
T23832 |
punct |
),Kd |
R6907 |
T23838 |
T23827 |
prep |
in,amount |
R6908 |
T23839 |
T23840 |
compound |
control,animals |
R6909 |
T23840 |
T23838 |
pobj |
animals,in |
R6910 |
T23841 |
T23790 |
punct |
.,Note |
R6911 |
T23843 |
T23844 |
compound |
Re,probing |
R6912 |
T23844 |
T23846 |
nsubj |
probing,confirmed |
R6913 |
T23845 |
T23844 |
punct |
-,probing |
R6914 |
T23847 |
T23844 |
prep |
with,probing |
R6915 |
T23848 |
T23849 |
det |
a,antibody |
R6916 |
T23849 |
T23847 |
pobj |
antibody,with |
R6917 |
T23850 |
T23851 |
compound |
c,myc |
R6918 |
T23851 |
T23849 |
compound |
myc,antibody |
R6919 |
T23852 |
T23851 |
punct |
-,myc |
R6920 |
T23853 |
T23849 |
compound |
tag,antibody |
R6921 |
T23854 |
T23855 |
det |
the,presence |
R6922 |
T23855 |
T23846 |
dobj |
presence,confirmed |
R6923 |
T23856 |
T23855 |
prep |
of,presence |
R6924 |
T23857 |
T23858 |
det |
the,E2 |
R6925 |
T23858 |
T23856 |
pobj |
E2,of |
R6926 |
T23859 |
T23860 |
npadvmod |
transgene,derived |
R6927 |
T23860 |
T23858 |
amod |
derived,E2 |
R6928 |
T23861 |
T23858 |
punct |
", ",E2 |
R6929 |
T23862 |
T23863 |
compound |
c,myc |
R6930 |
T23863 |
T23865 |
npadvmod |
myc,tagged |
R6931 |
T23864 |
T23863 |
punct |
-,myc |
R6932 |
T23865 |
T23858 |
amod |
tagged,E2 |
R6933 |
T23866 |
T23858 |
punct |
", ",E2 |
R6934 |
T23867 |
T23858 |
amod |
human,E2 |
R6935 |
T23868 |
T23846 |
prep |
in,confirmed |
R6936 |
T23869 |
T23870 |
amod |
transgenic,mice |
R6937 |
T23870 |
T23868 |
pobj |
mice,in |
R6938 |
T23871 |
T23868 |
cc |
but,in |
R6939 |
T23872 |
T23871 |
neg |
not,but |
R6940 |
T23873 |
T23868 |
conj |
in,in |
R6941 |
T23874 |
T23873 |
pobj |
controls,in |
R6942 |
T23875 |
T23846 |
punct |
.,confirmed |
R6943 |
T23877 |
T23878 |
compound |
Western,blot |
R6944 |
T23878 |
T23879 |
compound |
blot,analysis |
R6945 |
T23879 |
T23880 |
nsubj |
analysis,revealed |
R6946 |
T23881 |
T23879 |
prep |
of,analysis |
R6947 |
T23882 |
T23881 |
pobj |
brain,of |
R6948 |
T23883 |
T23884 |
punct |
(,C |
R6949 |
T23884 |
T23882 |
parataxis |
C,brain |
R6950 |
T23885 |
T23884 |
punct |
),C |
R6951 |
T23886 |
T23882 |
punct |
", ",brain |
R6952 |
T23887 |
T23882 |
conj |
kidney,brain |
R6953 |
T23888 |
T23889 |
punct |
(,D |
R6954 |
T23889 |
T23887 |
parataxis |
D,kidney |
R6955 |
T23890 |
T23889 |
punct |
),D |
R6956 |
T23891 |
T23887 |
punct |
", ",kidney |
R6957 |
T23892 |
T23887 |
cc |
and,kidney |
R6958 |
T23893 |
T23887 |
conj |
muscle,kidney |
R6959 |
T23894 |
T23895 |
punct |
(,E |
R6960 |
T23895 |
T23893 |
parataxis |
E,muscle |
R6961 |
T23896 |
T23895 |
punct |
),E |
R6962 |
T23897 |
T23898 |
amod |
negligible,amounts |
R6963 |
T23898 |
T23880 |
dobj |
amounts,revealed |
R6964 |
T23899 |
T23898 |
prep |
of,amounts |
R6965 |
T23900 |
T23901 |
npadvmod |
transgene,derived |
R6966 |
T23901 |
T23902 |
amod |
derived,E2 |
R6967 |
T23902 |
T23899 |
pobj |
E2,of |
R6968 |
T23903 |
T23880 |
prep |
in,revealed |
R6969 |
T23904 |
T23905 |
det |
those,tissues |
R6970 |
T23905 |
T23903 |
pobj |
tissues,in |
R6971 |
T23906 |
T23880 |
punct |
.,revealed |
R6972 |
T23908 |
T23909 |
det |
All,blots |
R6973 |
T23909 |
T23910 |
nsubjpass |
blots,re-probed |
R6974 |
T23911 |
T23910 |
auxpass |
were,re-probed |
R6975 |
T23912 |
T23910 |
prep |
with,re-probed |
R6976 |
T23913 |
T23914 |
det |
an,antibody |
R6977 |
T23914 |
T23912 |
pobj |
antibody,with |
R6978 |
T23915 |
T23914 |
compound |
actin,antibody |
R6979 |
T23916 |
T23917 |
aux |
to,allow |
R6980 |
T23917 |
T23910 |
advcl |
allow,re-probed |
R6981 |
T23918 |
T23919 |
nsubjpass |
amount,compared |
R6982 |
T23919 |
T23917 |
ccomp |
compared,allow |
R6983 |
T23920 |
T23918 |
prep |
of,amount |
R6984 |
T23921 |
T23920 |
pobj |
protein,of |
R6985 |
T23922 |
T23921 |
acl |
loaded,protein |
R6986 |
T23923 |
T23922 |
prep |
in,loaded |
R6987 |
T23924 |
T23925 |
det |
each,lane |
R6988 |
T23925 |
T23923 |
pobj |
lane,in |
R6989 |
T23926 |
T23919 |
aux |
to,compared |
R6990 |
T23927 |
T23919 |
auxpass |
be,compared |
R6991 |
T23928 |
T23910 |
punct |
.,re-probed |