PMC:1448208 / 22948-32301 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T24960	6289-6290	.	denotes	.
T24959	6286-6289	NN	denotes	SEM
T24958	6284-6285	SYM	denotes	-
T24957	6283-6284	HYPH	denotes	/
T24956	6282-6283	SYM	denotes	+
T24955	6277-6281	NN	denotes	mean
T24954	6273-6276	VBP	denotes	are
T24953	6266-6272	NNS	denotes	values
T24952	6262-6265	DT	denotes	All
T24951	6261-6290	sentence	denotes	All values are mean +/- SEM.
T24950	6260-6261	.	denotes	.
T24949	6257-6260	NN	denotes	age
T24948	6254-6256	IN	denotes	of
T24947	6248-6253	NNS	denotes	weeks
T24946	6245-6246	SYM	denotes	–
T24945	6246-6247	CD	denotes	6
T24944	6244-6245	CD	denotes	4
T24943	6239-6243	VBD	denotes	were
T24942	6234-6238	WDT	denotes	that
T24941	6229-6233	NNS	denotes	mice
T24497	4613-4616	NN	denotes	LAP
T24496	4609-4612	DT	denotes	the
T24495	4632-4642	NNS	denotes	transgenes
T24494	4604-4608	CC	denotes	both
T24493	4600-4603	IN	denotes	for
T24492	4591-4599	JJ	denotes	positive
T24491	4587-4590	CC	denotes	and
T24490	4584-4586	NN	denotes	E2
T24489	4573-4583	JJ	denotes	endogenous
T24482	4539-4540	-LRB-	denotes	(
T24481	4523-4529	NN	denotes	rescue
T24480	4530-4538	NN	denotes	genotype
T24479	4519-4522	DT	denotes	the
T24478	4515-4518	VBD	denotes	had
T24477	4510-4514	WDT	denotes	that
T24476	4503-4509	VBN	denotes	tested
T24475	4487-4497	JJ	denotes	transgenic
T24474	4498-4502	NN	denotes	line
T24473	4482-4486	DT	denotes	each
T24472	4477-4481	IN	denotes	from
T24471	4469-4476	VBG	denotes	weaning
T24470	4466-4468	IN	denotes	at
T24469	4460-4465	JJ	denotes	alive
T24468	4455-4459	NNS	denotes	mice
T24467	4452-4454	IN	denotes	of
T24466	4440-4451	NNS	denotes	percentages
T24465	4436-4439	VBP	denotes	are
T24464	4426-4435	VBN	denotes	Presented
T24463	4425-4644	sentence	denotes	Presented are percentages of mice alive at weaning from each transgenic line tested that had the rescue genotype (i.e., homozygous for knockout of endogenous E2 and positive for both the LAP-tTA and TRE-E2 transgenes).
T24462	4424-4425	.	denotes	.
T24461	4412-4418	NN	denotes	rescue
T24460	4419-4424	NNS	denotes	lines
T24459	4401-4411	JJ	denotes	transgenic
T24458	4398-4400	IN	denotes	of
T24457	4380-4388	NN	denotes	Survival
T24456	4378-4380	,	denotes	,
T24455	4389-4397	NN	denotes	analysis
T24454	4377-4378	LS	denotes	A
T24453	4376-4425	sentence	denotes	A, Survival analysis of transgenic rescue lines.
T24452	4375-4376	.	denotes	.
T24451	4363-4369	JJ	denotes	murine
T24450	4358-4362	NN	denotes	MSUD
T24449	4345-4357	JJ	denotes	intermediate
T24448	4370-4375	NN	denotes	model
T24447	4341-4344	DT	denotes	the
T24446	4338-4340	IN	denotes	of
T24445	4321-4337	NN	denotes	Characterization
T24940	6224-6228	IN	denotes	from
T24939	6209-6213	VBD	denotes	were
T24938	6214-6223	VBN	denotes	collected
T24937	6201-6208	NNS	denotes	Samples
T24936	6200-6261	sentence	denotes	Samples were collected from mice that were 4–6 weeks of age.
T24935	6199-6200	.	denotes	.
T24934	6182-6186	NN	denotes	mass
T24933	6187-6199	NN	denotes	spectrometry
T24932	6175-6181	JJ	denotes	tandem
T24931	6172-6174	IN	denotes	by
T24930	6161-6171	VBN	denotes	determined
T24929	6158-6160	IN	denotes	as
T24928	6149-6157	NNS	denotes	controls
T24927	6138-6148	NN	denotes	littermate
T24926	6134-6137	CC	denotes	and
T24925	6123-6127	NN	denotes	MSUD
T24924	6110-6122	JJ	denotes	intermediate
T24923	6128-6133	NN	denotes	model
T24922	6106-6109	DT	denotes	the
T24921	6103-6105	IN	denotes	in
T24920	6091-6095	NN	denotes	acid
T24919	6085-6090	NN	denotes	amino
T24918	6079-6084	NN	denotes	blood
T24917	6096-6102	NNS	denotes	levels
T24916	6068-6078	JJ	denotes	additional
T24915	6065-6067	IN	denotes	of
T24914	6057-6064	NN	denotes	Summary
T24680	5433-5443	VBN	denotes	sacrificed
T24679	5420-5432	RB	denotes	subsequently
T24678	5416-5419	CC	denotes	and
T24677	5407-5415	JJ	denotes	moribund
T24676	5397-5401	PRP	denotes	they
T24675	5402-5406	VBD	denotes	were
T24674	5391-5396	IN	denotes	until
T24673	5376-5380	VBD	denotes	were
T24672	5368-5375	NN	denotes	weaning
T24671	5365-5367	IN	denotes	at
T24670	5359-5364	JJ	denotes	alive
T24669	5381-5390	VBN	denotes	monitored
T24668	5354-5358	NNS	denotes	mice
T24667	5347-5353	NN	denotes	Rescue
T24666	5346-5478	sentence	denotes	Rescue mice alive at weaning were monitored until they were moribund and subsequently sacrificed or were found dead in their cages.
T24665	5345-5346	.	denotes	.
T24664	5341-5345	NN	denotes	525A
T24663	5337-5340	CC	denotes	and
T24662	5335-5336	NN	denotes	A
T24661	5329-5334	NNS	denotes	Lines
T24660	5324-5328	IN	denotes	from
T24659	5319-5323	NNS	denotes	mice
T24658	5315-5318	IN	denotes	for
T24657	5299-5307	NN	denotes	Survival
T24656	5297-5299	,	denotes	,
T24655	5308-5314	NNS	denotes	curves
T24654	5296-5297	LS	denotes	D
T24653	5295-5346	sentence	denotes	D, Survival curves for mice from Lines A and 525A.
T24652	5294-5295	.	denotes	.
T24651	5286-5287	SYM	denotes	≤
T24650	5284-5285	NN	denotes	P
T24649	5282-5284	,	denotes	,
T24648	5280-5282	SYM	denotes	**
T24647	5278-5279	:	denotes	;
T24646	5288-5294	CD	denotes	0.0001
T24645	5271-5272	SYM	denotes	≤
T24644	5269-5270	NN	denotes	P
T24643	5267-5269	,	denotes	,
T24642	5273-5278	CD	denotes	0.001
T24641	5266-5267	SYM	denotes	*
T24640	5265-5295	sentence	denotes	, P ≤ 0.001; *, P ≤ 0.0001.
T24639	5264-5265	.	denotes	.
T24638	5255-5259	NN	denotes	525A
T24637	5251-5254	CC	denotes	and
T24636	5249-5250	NN	denotes	A
T24635	5243-5248	NNS	denotes	Lines
T24634	5239-5242	CC	denotes	and
T24633	5260-5264	NNS	denotes	mice
T24632	5231-5238	NN	denotes	control
T24631	5228-5230	IN	denotes	of
T24630	5222-5227	NN	denotes	liver
T24629	5219-5221	IN	denotes	in
T24628	5203-5209	NN	denotes	enzyme
T24627	5197-5202	NN	denotes	BCKDH
T24626	5195-5197	,	denotes	,
T24625	5210-5218	NN	denotes	activity
T24624	5194-5195	LS	denotes	C
T24623	5193-5265	sentence	denotes	C, BCKDH enzyme activity in liver of control and Lines A and 525A mice.
T24622	5192-5193	.	denotes	.
T24621	5185-5186	SYM	denotes	<
T24620	5183-5184	NN	denotes	P
T24619	5181-5183	,	denotes	,
T24618	5179-5181	SYM	denotes	**
T24617	5177-5178	:	denotes	;
T24616	5187-5192	CD	denotes	0.005
T24615	5171-5172	SYM	denotes	≤
T24614	5169-5170	NN	denotes	P
T24613	5167-5169	,	denotes	,
T24612	5173-5177	CD	denotes	0.01
T24611	5166-5167	SYM	denotes	*
T24610	5165-5193	sentence	denotes	, P ≤ 0.01; *, P < 0.005.
T24609	5164-5165	.	denotes	.
T24608	5156-5164	VBN	denotes	analyzed
T24607	5148-5155	NNS	denotes	samples
T24606	5145-5147	IN	denotes	of
T24605	5137-5144	NNS	denotes	numbers
T24604	5133-5136	DT	denotes	the
T24603	5118-5122	NNS	denotes	bars
T24602	5114-5117	DT	denotes	the
T24601	5111-5113	IN	denotes	on
T24600	5123-5132	VBZ	denotes	indicates
T24599	5103-5110	NNS	denotes	numbers
T24598	5099-5102	DT	denotes	The
T24597	5098-5165	sentence	denotes	The numbers on the bars indicates the numbers of samples analyzed.
T24596	5097-5098	.	denotes	.
T24595	5091-5097	VBN	denotes	tested
T24594	5086-5090	NNS	denotes	ages
T24593	5082-5085	DT	denotes	all
T24592	5079-5081	IN	denotes	at
T24591	5070-5078	NNS	denotes	controls
T24590	5067-5069	IN	denotes	to
T24589	5058-5066	VBN	denotes	compared
T24588	5053-5057	NN	denotes	525A
T24587	5049-5052	CC	denotes	and
T24586	5047-5048	NN	denotes	A
T24585	5041-5046	NNS	denotes	Lines
T24584	5037-5040	IN	denotes	for
T24583	5029-5036	JJR	denotes	greater
T24582	5015-5028	RB	denotes	significantly
T24581	5010-5014	VBD	denotes	were
T24580	5003-5009	NNS	denotes	values
T24579	4994-4995	HYPH	denotes	/
T24578	4995-5002	NN	denotes	alanine
T24577	4990-4994	NN	denotes	BCAA
T24576	4989-5098	sentence	denotes	BCAA/alanine values were significantly greater for Lines A and 525A compared to controls at all ages tested.
T24575	4988-4989	.	denotes	.
T24574	4982-4988	NN	denotes	valine
T24573	4978-4981	CC	denotes	and
T24572	4976-4978	,	denotes	,
T24571	4966-4976	NN	denotes	isoleucine
T24570	4964-4966	,	denotes	,
T24569	4957-4964	NN	denotes	leucine
T24568	4954-4956	IN	denotes	of
T24567	4950-4953	NN	denotes	sum
T24566	4946-4949	DT	denotes	the
T24565	4936-4945	VBP	denotes	represent
T24564	4931-4935	NN	denotes	BCAA
T24563	4925-4930	JJ	denotes	Total
T24562	4924-4989	sentence	denotes	Total BCAA represent the sum of leucine, isoleucine, and valine.
T24561	4923-4924	.	denotes	.
T24560	4919-4923	NN	denotes	525A
T24559	4915-4918	CC	denotes	and
T24558	4913-4914	NN	denotes	A
T24557	4907-4912	NNS	denotes	Lines
T24556	4903-4906	CC	denotes	and
T24555	4894-4902	NNS	denotes	controls
T24554	4889-4893	IN	denotes	from
T24553	4884-4888	NNS	denotes	mice
T24552	4881-4883	IN	denotes	of
T24551	4875-4880	NN	denotes	blood
T24550	4872-4874	IN	denotes	in
T24549	4864-4871	NN	denotes	alanine
T24548	4861-4863	IN	denotes	to
T24547	4856-4860	NN	denotes	BCAA
T24546	4850-4855	JJ	denotes	total
T24545	4847-4849	IN	denotes	of
T24544	4839-4841	,	denotes	,
T24543	4841-4846	NN	denotes	Ratio
T24542	4838-4839	LS	denotes	B
T24541	4837-4924	sentence	denotes	B, Ratio of total BCAA to alanine in blood of mice from controls and Lines A and 525A.
T24540	4836-4837	.	denotes	.
T24539	4832-4836	NN	denotes	line
T24538	4827-4831	DT	denotes	each
T24537	4823-4826	IN	denotes	for
T24536	4810-4822	NNS	denotes	observations
T24535	4807-4809	IN	denotes	of
T24534	4799-4806	NNS	denotes	numbers
T24533	4795-4798	DT	denotes	the
T24532	4780-4784	NNS	denotes	bars
T24531	4776-4779	DT	denotes	the
T24530	4773-4775	IN	denotes	on
T24529	4785-4794	VBZ	denotes	indicates
T24528	4765-4772	NNS	denotes	numbers
T24527	4761-4764	DT	denotes	The
T24526	4760-4837	sentence	denotes	The numbers on the bars indicates the numbers of observations for each line.
T24525	4759-4760	.	denotes	.
T24524	4752-4759	NNS	denotes	animals
T24523	4749-4751	IN	denotes	of
T24522	4738-4748	NN	denotes	population
T24521	4733-4737	DT	denotes	this
T24520	4730-4732	IN	denotes	in
T24519	4718-4720	VBZ	denotes	is
T24518	4709-4717	NN	denotes	genotype
T24517	4704-4708	DT	denotes	this
T24516	4698-4703	WDT	denotes	which
T24515	4721-4729	VBN	denotes	expected
T24514	4695-4697	IN	denotes	at
T24513	4677-4684	JJ	denotes	maximum
T24512	4665-4676	JJ	denotes	theoretical
T24511	4685-4694	NN	denotes	frequency
T24510	4661-4664	DT	denotes	the
T24509	4650-4657	VBN	denotes	plotted
T24508	4658-4660	VBZ	denotes	is
T24507	4645-4649	RB	denotes	Also
T24506	4644-4760	sentence	denotes	Also plotted is the theoretical maximum frequency at which this genotype is expected in this population of animals.
T24505	4643-4644	.	denotes	.
T24504	4642-4643	-RRB-	denotes	)
T24503	4628-4629	HYPH	denotes	-
T24502	4629-4631	NN	denotes	E2
T24501	4625-4628	NN	denotes	TRE
T24500	4621-4624	CC	denotes	and
T24499	4616-4617	HYPH	denotes	-
T24498	4617-4620	NN	denotes	tTA
T24688	5477-5478	.	denotes	.
T23927	2163-2165	VB	denotes	be
T23926	2160-2162	TO	denotes	to
T23925	2155-2159	NN	denotes	lane
T23924	2150-2154	DT	denotes	each
T23923	2147-2149	IN	denotes	in
T23922	2140-2146	VBN	denotes	loaded
T23921	2132-2139	NN	denotes	protein
T23920	2129-2131	IN	denotes	of
T23919	2166-2174	VBN	denotes	compared
T23918	2122-2128	NN	denotes	amount
T23917	2116-2121	VB	denotes	allow
T23916	2113-2115	TO	denotes	to
T23915	2098-2103	NN	denotes	actin
T23914	2104-2112	NN	denotes	antibody
T23913	2095-2097	DT	denotes	an
T23912	2090-2094	IN	denotes	with
T23911	2075-2079	VBD	denotes	were
T23910	2080-2089	VBN	denotes	re-probed
T23909	2069-2074	NNS	denotes	blots
T23908	2065-2068	DT	denotes	All
T23907	2064-2175	sentence	denotes	All blots were re-probed with an actin antibody to allow amount of protein loaded in each lane to be compared.
T23906	2063-2064	.	denotes	.
T23905	2056-2063	NNS	denotes	tissues
T23904	2050-2055	DT	denotes	those
T23903	2047-2049	IN	denotes	in
T23902	2044-2046	NN	denotes	E2
T23901	2036-2043	VBN	denotes	derived
T23900	2026-2035	NN	denotes	transgene
T23899	2023-2025	IN	denotes	of
T23898	2015-2022	NNS	denotes	amounts
T23897	2004-2014	JJ	denotes	negligible
T23896	1993-1994	-RRB-	denotes	)
T23895	1992-1993	NN	denotes	E
T23894	1991-1992	-LRB-	denotes	(
T23893	1984-1990	NN	denotes	muscle
T23892	1980-1983	CC	denotes	and
T23891	1978-1980	,	denotes	,
T23890	1977-1978	-RRB-	denotes	)
T23889	1976-1977	NN	denotes	D
T23888	1975-1976	-LRB-	denotes	(
T23887	1968-1974	NN	denotes	kidney
T23886	1966-1968	,	denotes	,
T23885	1965-1966	-RRB-	denotes	)
T23884	1964-1965	NN	denotes	C
T23883	1963-1964	-LRB-	denotes	(
T23882	1957-1962	NN	denotes	brain
T23881	1954-1956	IN	denotes	of
T23880	1995-2003	VBD	denotes	revealed
T23879	1945-1953	NN	denotes	analysis
T23878	1940-1944	NN	denotes	blot
T23877	1932-1939	NNP	denotes	Western
T23876	1931-2064	sentence	denotes	Western blot analysis of brain (C), kidney (D), and muscle (E) revealed negligible amounts of transgene derived E2 in those tissues.
T23875	1930-1931	.	denotes	.
T23874	1922-1930	NNS	denotes	controls
T23873	1919-1921	IN	denotes	in
T23872	1915-1918	RB	denotes	not
T23871	1911-1914	CC	denotes	but
T23870	1906-1910	NNS	denotes	mice
T23869	1895-1905	JJ	denotes	transgenic
T23868	1892-1894	IN	denotes	in
T23867	1883-1888	JJ	denotes	human
T23866	1881-1883	,	denotes	,
T23865	1875-1881	VBN	denotes	tagged
T23864	1870-1871	HYPH	denotes	-
T23863	1871-1874	NN	denotes	myc
T23862	1869-1870	NN	denotes	c
T23861	1867-1869	,	denotes	,
T23860	1860-1867	VBN	denotes	derived
T23859	1850-1859	NN	denotes	transgene
T23858	1889-1891	NN	denotes	E2
T23857	1846-1849	DT	denotes	the
T23856	1843-1845	IN	denotes	of
T23855	1834-1842	NN	denotes	presence
T23854	1830-1833	DT	denotes	the
T23853	1807-1810	NN	denotes	tag
T23852	1802-1803	HYPH	denotes	-
T23851	1803-1806	NN	denotes	myc
T23850	1801-1802	NN	denotes	c
T23849	1811-1819	NN	denotes	antibody
T23848	1799-1800	DT	denotes	a
T23847	1794-1798	IN	denotes	with
T23846	1820-1829	VBD	denotes	confirmed
T23845	1785-1786	HYPH	denotes	-
T23844	1786-1793	NN	denotes	probing
T23843	1783-1785	NN	denotes	Re
T23842	1782-1931	sentence	denotes	Re-probing with a c-myc tag antibody confirmed the presence of the transgene derived, c-myc tagged, human E2 in transgenic mice but not in controls.
T23841	1781-1782	.	denotes	.
T23840	1774-1781	NNS	denotes	animals
T23839	1766-1773	NN	denotes	control
T23838	1763-1765	IN	denotes	in
T23837	1761-1762	-RRB-	denotes	)
T23836	1756-1758	CD	denotes	47
T23835	1755-1756	SYM	denotes	~
T23834	1754-1755	SYM	denotes	=
T23833	1752-1754	NN	denotes	MW
T23832	1759-1761	NN	denotes	Kd
T23831	1751-1752	-LRB-	denotes	(
T23830	1748-1750	NN	denotes	E2
T23829	1742-1747	NN	denotes	mouse
T23828	1739-1741	IN	denotes	of
T23827	1732-1738	NN	denotes	amount
T23826	1728-1731	DT	denotes	the
T23825	1725-1727	IN	denotes	to
T23824	1717-1724	JJ	denotes	similar
T23823	1706-1712	NN	denotes	amount
T23822	1702-1705	DT	denotes	the
T23821	1694-1701	NNS	denotes	animals
T23820	1690-1693	DT	denotes	the
T23819	1687-1689	IN	denotes	of
T23818	1682-1686	JJ	denotes	many
T23817	1713-1716	VBD	denotes	was
T23816	1679-1681	IN	denotes	in
T23815	1675-1678	CC	denotes	but
T23814	1666-1674	JJ	denotes	variable
T23813	1660-1661	NN	denotes	A
T23812	1656-1659	CD	denotes	525
T23811	1652-1655	CC	denotes	and
T23810	1650-1651	NN	denotes	A
T23809	1644-1649	NNS	denotes	Lines
T23808	1639-1643	IN	denotes	from
T23807	1634-1638	NNS	denotes	mice
T23806	1631-1633	IN	denotes	in
T23805	1629-1630	-RRB-	denotes	)
T23804	1627-1629	NN	denotes	Kd
T23803	1624-1626	CD	denotes	54
T23802	1623-1624	SYM	denotes	~
T23801	1610-1619	VBN	denotes	predicted
T23800	1620-1622	NN	denotes	MW
T23799	1609-1610	-LRB-	denotes	(
T23798	1598-1600	NN	denotes	E2
T23797	1601-1608	NN	denotes	protein
T23796	1592-1597	JJ	denotes	human
T23795	1589-1591	IN	denotes	of
T23794	1582-1588	NN	denotes	amount
T23793	1578-1581	DT	denotes	the
T23792	1662-1665	VBD	denotes	was
T23791	1573-1577	IN	denotes	that
T23790	1568-1572	VB	denotes	Note
T23789	1567-1782	sentence	denotes	Note that the amount of human E2 protein (predicted MW ~54 Kd) in mice from Lines A and 525 A was variable but in many of the animals the amount was similar to the amount of mouse E2 (MW=~47 Kd) in control animals.
T23788	1566-1567	.	denotes	.
T23787	1557-1561	NN	denotes	MSUD
T23786	1544-1556	JJ	denotes	intermediate
T23785	1540-1543	CC	denotes	and
T23784	1562-1566	NNS	denotes	mice
T23783	1532-1539	NN	denotes	control
T23782	1529-1531	IN	denotes	of
T23781	1523-1528	NN	denotes	liver
T23780	1520-1522	IN	denotes	in
T23779	1512-1519	NN	denotes	protein
T23778	1509-1511	NN	denotes	E2
T23777	1506-1508	IN	denotes	of
T23776	1492-1496	NN	denotes	blot
T23775	1484-1491	NNP	denotes	Western
T23774	1482-1484	,	denotes	,
T23773	1497-1505	NN	denotes	analysis
T23772	1481-1482	LS	denotes	B
T23771	1480-1567	sentence	denotes	B, Western blot analysis of E2 protein in liver of control and intermediate MSUD mice.
T23770	1479-1480	.	denotes	.
T23769	1475-1479	NNS	denotes	mice
T23768	1464-1474	JJ	denotes	transgenic
T23767	1461-1463	IN	denotes	of
T23766	1455-1460	NNS	denotes	lines
T23765	1447-1454	JJ	denotes	several
T23764	1440-1446	VB	denotes	create
T23763	1437-1439	TO	denotes	to
T23762	1428-1431	VBD	denotes	was
T23761	1432-1436	VBN	denotes	used
T23760	1418-1427	NN	denotes	construct
T23759	1413-1417	DT	denotes	This
T23758	1412-1480	sentence	denotes	This construct was used to create several lines of transgenic mice.
T23757	1411-1412	.	denotes	.
T23756	1387-1402	NN	denotes	polyadenylation
T23755	1403-1411	NN	denotes	sequence
T23754	1379-1386	VBN	denotes	derived
T23753	1374-1378	NN	denotes	SV40
T23752	1370-1373	CC	denotes	and
T23751	1368-1370	,	denotes	,
T23750	1357-1364	NN	denotes	epitope
T23749	1352-1353	HYPH	denotes	-
T23748	1353-1356	NN	denotes	myc
T23747	1351-1352	NN	denotes	c
T23746	1365-1368	NN	denotes	tag
T23745	1349-1350	DT	denotes	a
T23744	1347-1349	,	denotes	,
T23743	1333-1340	NN	denotes	alanine
T23742	1341-1347	NN	denotes	spacer
T23741	1330-1332	DT	denotes	an
T23740	1328-1330	,	denotes	,
T23739	1321-1323	NN	denotes	E2
T23738	1315-1320	JJ	denotes	human
T23737	1324-1328	NN	denotes	cDNA
T23736	1311-1314	DT	denotes	the
T23735	1309-1311	,	denotes	,
T23734	1308-1309	-RRB-	denotes	)
T23733	1299-1303	JJ	denotes	thin
T23732	1304-1308	NN	denotes	line
T23731	1298-1299	-LRB-	denotes	(
T23730	1281-1290	JJ	denotes	synthetic
T23729	1291-1297	NN	denotes	intron
T23728	1279-1280	DT	denotes	a
T23727	1277-1279	,	denotes	,
T23726	1269-1277	NN	denotes	promoter
T23725	1265-1268	DT	denotes	the
T23724	1262-1264	IN	denotes	of
T23723	1257-1261	NN	denotes	part
T23722	1254-1256	IN	denotes	as
T23721	1252-1253	-RRB-	denotes	)
T23720	1249-1252	NN	denotes	TRE
T23719	1248-1249	-LRB-	denotes	(
T23718	1231-1239	NN	denotes	response
T23717	1218-1230	NN	denotes	tetracycline
T23716	1240-1247	NN	denotes	element
T23715	1214-1217	DT	denotes	the
T23714	1205-1213	VBZ	denotes	contains
T23713	1191-1192	HYPH	denotes	-
T23712	1192-1194	NN	denotes	E2
T23711	1188-1191	NN	denotes	TRE
T23710	1195-1204	NN	denotes	transgene
T23709	1184-1187	DT	denotes	The
T23708	1183-1412	sentence	denotes	The TRE-E2 transgene contains the tetracycline response element (TRE) as part of the promoter, a synthetic intron (thin line), the human E2 cDNA, an alanine spacer, a c-myc epitope tag, and SV40 derived polyadenylation sequence.
T23707	1182-1183	.	denotes	.
T23706	1170-1173	NN	denotes	LAP
T23705	1161-1169	JJ	denotes	specific
T23704	1155-1160	NN	denotes	liver
T23703	1174-1182	NN	denotes	promoter
T23702	1151-1154	DT	denotes	the
T23701	1146-1150	IN	denotes	from
T23700	1144-1145	-RRB-	denotes	)
T23699	1141-1144	NN	denotes	tTA
T23698	1140-1141	-LRB-	denotes	(
T23697	1113-1114	HYPH	denotes	-
T23696	1114-1124	VBN	denotes	controlled
T23695	1101-1113	NN	denotes	tetracycline
T23694	1125-1139	NN	denotes	transactivator
T23693	1097-1100	DT	denotes	the
T23692	1089-1096	VBP	denotes	express
T23691	1084-1088	NNS	denotes	mice
T23690	1078-1083	DT	denotes	These
T23689	1077-1183	sentence	denotes	These mice express the tetracycline-controlled transactivator (tTA) from the liver specific LAP promoter.
T23688	1076-1077	.	denotes	.
T23687	1075-1076	-RRB-	denotes	]
T23686	1073-1075	CD	denotes	34
T23685	1072-1073	-LRB-	denotes	[
T23684	1051-1061	RB	denotes	previously
T23683	1046-1050	VBN	denotes	been
T23682	1041-1045	VBP	denotes	have
T23681	1062-1071	VBN	denotes	described
T23680	1025-1035	JJ	denotes	transgenic
T23679	1036-1040	NNS	denotes	mice
T23678	1020-1021	HYPH	denotes	-
T23677	1021-1024	NN	denotes	tTA
T23676	1017-1020	NN	denotes	LAP
T23675	1016-1077	sentence	denotes	LAP-tTA transgenic mice have been previously described [34].
T23674	1015-1016	.	denotes	.
T23673	1013-1015	NN	denotes	E2
T23672	1007-1012	JJ	denotes	human
T23671	999-1006	VBP	denotes	express
T23670	994-998	WDT	denotes	that
T23669	989-993	NNS	denotes	mice
T23668	981-988	VB	denotes	produce
T23667	978-980	TO	denotes	to
T23666	973-977	VBN	denotes	used
T23665	953-963	JJ	denotes	Transgenic
T23664	951-953	,	denotes	,
T23663	964-972	NN	denotes	strategy
T23662	950-951	LS	denotes	A
T23661	949-1016	sentence	denotes	A, Transgenic strategy used to produce mice that express human E2.
T23660	948-949	.	denotes	.
T23659	932-948	NN	denotes	characterization
T23658	928-931	CC	denotes	and
T23657	911-916	NN	denotes	mouse
T23656	917-927	NN	denotes	production
T23655	900-910	JJ	denotes	Transgenic
T16742	8024-8025	-LRB-	denotes	[
T16825	8401-8402	.	denotes	.
T16824	8394-8401	NNS	denotes	tissues
T16823	8388-8393	DT	denotes	those
T16822	8385-8387	IN	denotes	in
T16821	8374-8384	JJ	denotes	negligible
T16820	8368-8370	NN	denotes	E2
T16819	8365-8367	IN	denotes	of
T16818	8354-8364	NN	denotes	expression
T16817	8352-8354	,	denotes	,
T16816	8340-8352	RB	denotes	respectively
T16815	8337-8339	NN	denotes	3E
T16814	8333-8336	CC	denotes	and
T16813	8331-8332	NN	denotes	D
T16812	8329-8331	,	denotes	,
T16811	8327-8329	NN	denotes	3C
T16810	8320-8326	NN	denotes	Figure
T16809	8317-8319	IN	denotes	in
T16808	8371-8373	VBZ	denotes	is
T16807	8311-8316	VBN	denotes	shown
T16806	8308-8310	IN	denotes	As
T16805	8307-8402	sentence	denotes	As shown in Figure 3C, D and 3E respectively, expression of E2 is negligible in those tissues.
T16804	8306-8307	.	denotes	.
T16803	8300-8306	NN	denotes	muscle
T16802	8296-8299	CC	denotes	and
T16801	8289-8295	NN	denotes	kidney
T16800	8287-8289	,	denotes	,
T16799	8282-8287	NN	denotes	brain
T16798	8279-8281	IN	denotes	in
T16797	8266-8268	NN	denotes	E2
T16796	8269-8278	NN	denotes	transgene
T16795	8262-8265	DT	denotes	the
T16794	8259-8261	IN	denotes	of
T16793	8248-8258	NN	denotes	expression
T16792	8240-8247	VB	denotes	analyze
T16791	8237-8239	TO	denotes	to
T16790	8228-8236	NN	denotes	analysis
T16789	8223-8227	NN	denotes	blot
T16788	8215-8222	NNP	denotes	western
T16787	8205-8209	RB	denotes	also
T16786	8210-8214	VBD	denotes	used
T16785	8202-8204	PRP	denotes	We
T16784	8201-8307	sentence	denotes	We also used western blot analysis to analyze expression of the E2 transgene in brain, kidney and muscle.
T16783	8200-8201	.	denotes	.
T16782	8190-8194	NN	denotes	acid
T16781	8184-8189	NN	denotes	amino
T16780	8195-8200	NN	denotes	level
T16779	8180-8183	DT	denotes	the
T16778	8177-8179	IN	denotes	at
T16777	8174-8176	NN	denotes	E2
T16776	8168-8173	NN	denotes	mouse
T16775	8165-8167	IN	denotes	to
T16774	8156-8164	NN	denotes	identity
T16773	8154-8155	NN	denotes	%
T16772	8152-8154	CD	denotes	88
T16771	8151-8152	SYM	denotes	~
T16770	8144-8150	VBZ	denotes	shares
T16769	8141-8143	NN	denotes	E2
T16768	8135-8140	JJ	denotes	Human
T16767	8134-8201	sentence	denotes	Human E2 shares ~88% identity to mouse E2 at the amino acid level.
T16766	8133-8134	.	denotes	.
T16765	8125-8133	NNS	denotes	subunits
T16764	8122-8124	NN	denotes	E3
T16763	8118-8121	CC	denotes	and
T16762	8115-8117	NN	denotes	E1
T16761	8109-8114	NN	denotes	mouse
T16760	8104-8108	IN	denotes	with
T16759	8094-8103	VBN	denotes	complexed
T16758	8089-8093	WRB	denotes	when
T16757	8078-8088	JJ	denotes	functional
T16756	8072-8077	RB	denotes	fully
T16755	8068-8071	RB	denotes	not
T16754	8061-8063	NN	denotes	E2
T16753	8055-8060	JJ	denotes	human
T16752	8064-8067	VBD	denotes	was
T16751	8050-8054	IN	denotes	that
T16750	8041-8049	JJ	denotes	possible
T16749	8036-8040	RB	denotes	also
T16748	8033-8035	VBZ	denotes	is
T16747	8030-8032	PRP	denotes	It
T16746	8029-8134	sentence	denotes	It is also possible that human E2 was not fully functional when complexed with mouse E1 and E3 subunits.
T16745	8028-8029	.	denotes	.
T16744	8027-8028	-RRB-	denotes	]
T16743	8025-8027	CD	denotes	36
T16741	8007-8014	NN	denotes	subunit
T16740	8015-8023	NN	denotes	assembly
T16739	8000-8006	JJ	denotes	normal
T16738	7995-7999	IN	denotes	with
T16737	7971-7975	FW	denotes	coli
T16736	7968-7970	FW	denotes	E.
T16735	7958-7967	JJ	denotes	analogous
T16734	7976-7983	NN	denotes	subunit
T16733	7955-7957	DT	denotes	an
T16732	7952-7954	IN	denotes	of
T16731	7935-7942	NN	denotes	carboxy
T16730	7943-7951	NN	denotes	terminus
T16729	7931-7934	DT	denotes	the
T16728	7928-7930	IN	denotes	on
T16727	7918-7923	NN	denotes	Hisx6
T16726	7924-7927	NN	denotes	tag
T16725	7916-7917	DT	denotes	a
T16724	7913-7915	IN	denotes	of
T16723	7984-7994	VBD	denotes	interfered
T16722	7903-7912	NN	denotes	insertion
T16721	7899-7902	CC	denotes	and
T16720	7897-7898	-RRB-	denotes	]
T16719	7895-7897	CD	denotes	35
T16718	7894-7895	-LRB-	denotes	[
T16717	7881-7893	NNS	denotes	interactions
T16716	7873-7880	NN	denotes	subunit
T16715	7869-7872	IN	denotes	for
T16714	7859-7868	JJ	denotes	essential
T16713	7834-7847	NN	denotes	dehydrogenase
T16712	7825-7833	NN	denotes	pyruvate
T16711	7848-7855	NN	denotes	complex
T16710	7821-7824	DT	denotes	the
T16709	7818-7820	IN	denotes	of
T16708	7800-7809	JJ	denotes	analogous
T16707	7810-7817	NN	denotes	subunit
T16706	7797-7799	DT	denotes	an
T16705	7794-7796	IN	denotes	of
T16704	7777-7784	NN	denotes	carboxy
T16703	7785-7793	NN	denotes	terminus
T16702	7773-7776	DT	denotes	the
T16701	7856-7858	VBZ	denotes	is
T16700	7768-7772	IN	denotes	that
T16699	7754-7758	VBP	denotes	have
T16698	7759-7767	VBN	denotes	revealed
T16697	7748-7753	WDT	denotes	which
T16696	7740-7747	NNS	denotes	studies
T16695	7731-7739	JJ	denotes	previous
T16694	7726-7730	IN	denotes	with
T16693	7715-7725	JJ	denotes	consistent
T16692	7712-7714	VBZ	denotes	is
T16691	7697-7711	NN	denotes	interpretation
T16690	7692-7696	DT	denotes	This
T16689	7691-8029	sentence	denotes	This interpretation is consistent with previous studies which have revealed that the carboxy terminus of an analogous subunit of the pyruvate dehydrogenase complex is essential for subunit interactions [35] and insertion of a Hisx6 tag on the carboxy terminus of an analogous E. coli subunit interfered with normal subunit assembly [36].
T16688	7690-7691	.	denotes	.
T16687	7682-7690	NN	denotes	activity
T16686	7672-7681	JJ	denotes	enzymatic
T16685	7667-7671	IN	denotes	with
T16684	7647-7652	JJ	denotes	human
T16683	7639-7646	VBN	denotes	derived
T16682	7629-7638	NN	denotes	transgene
T16681	7653-7655	NN	denotes	E2
T16680	7625-7628	DT	denotes	the
T16679	7622-7624	IN	denotes	of
T16678	7605-7612	NN	denotes	carboxy
T16677	7613-7621	NN	denotes	terminus
T16676	7601-7604	DT	denotes	the
T16675	7598-7600	IN	denotes	at
T16674	7589-7590	HYPH	denotes	-
T16673	7590-7593	NN	denotes	myc
T16672	7588-7589	NN	denotes	c
T16671	7594-7597	NN	denotes	tag
T16670	7584-7587	DT	denotes	the
T16669	7656-7666	VBD	denotes	interfered
T16668	7579-7583	IN	denotes	that
T16667	7570-7578	JJ	denotes	probable
T16666	7567-7569	VBZ	denotes	is
T16665	7564-7566	PRP	denotes	It
T16664	7563-7691	sentence	denotes	It is probable that the c-myc tag at the carboxy terminus of the transgene derived human E2 interfered with enzymatic activity.
T16663	7562-7563	.	denotes	.
T16662	7546-7556	JJ	denotes	suboptimal
T16661	7557-7562	NN	denotes	level
T16660	7544-7545	DT	denotes	a
T16659	7541-7543	IN	denotes	at
T16658	7526-7528	VBZ	denotes	is
T16657	7515-7522	VBN	denotes	derived
T16656	7523-7525	NN	denotes	E2
T16655	7505-7514	NN	denotes	transgene
T16654	7529-7540	VBG	denotes	functioning
T16653	7500-7504	IN	denotes	that
T16652	7476-7482	NN	denotes	enzyme
T16651	7470-7475	NN	denotes	BCKDH
T16650	7483-7491	NN	denotes	activity
T16649	7463-7469	JJ	denotes	normal
T16648	7460-7462	IN	denotes	of
T16647	7458-7459	NN	denotes	%
T16646	7456-7457	SYM	denotes	–
T16645	7455-7456	CD	denotes	5
T16644	7454-7455	SYM	denotes	~
T16643	7457-7458	CD	denotes	6
T16642	7449-7453	RB	denotes	only
T16641	7446-7448	IN	denotes	in
T16640	7431-7438	NN	denotes	protein
T16639	7428-7430	NN	denotes	E2
T16638	7425-7427	IN	denotes	of
T16637	7410-7416	JJ	denotes	normal
T16636	7405-7409	RB	denotes	near
T16635	7417-7424	NNS	denotes	amounts
T16634	7399-7404	DT	denotes	these
T16633	7439-7445	VBP	denotes	result
T16632	7394-7398	IN	denotes	that
T16631	7492-7499	VBP	denotes	suggest
T16630	7381-7393	NNS	denotes	observations
T16629	7377-7380	DT	denotes	The
T16628	7376-7563	sentence	denotes	The observations that these near normal amounts of E2 protein result in only ~5–6% of normal BCKDH enzyme activity suggest that transgene derived E2 is functioning at a suboptimal level.
T16627	7375-7376	.	denotes	.
T16626	7363-7370	NN	denotes	control
T16625	7371-7375	NNS	denotes	mice
T16624	7349-7362	JJ	denotes	nontransgenic
T16623	7346-7348	IN	denotes	of
T16622	7340-7345	NN	denotes	liver
T16621	7337-7339	IN	denotes	in
T16620	7328-7336	VBN	denotes	produced
T16619	7326-7327	-RRB-	denotes	)
T16618	7321-7323	CD	denotes	47
T16617	7320-7321	SYM	denotes	~
T16616	7324-7326	NN	denotes	Kd
T16615	7319-7320	-LRB-	denotes	(
T16614	7316-7318	NN	denotes	E2
T16613	7310-7315	NN	denotes	mouse
T16612	7307-7309	IN	denotes	of
T16611	7300-7306	NN	denotes	amount
T16610	7296-7299	DT	denotes	the
T16529	6929-6932	VBD	denotes	was
T16528	6924-6928	NN	denotes	525A
T16527	6920-6923	CC	denotes	and
T16526	6918-6919	NN	denotes	A
T16525	6912-6917	NNS	denotes	Lines
T16524	6909-6911	IN	denotes	of
T16523	6903-6908	NN	denotes	liver
T16522	6900-6902	IN	denotes	in
T16521	6889-6891	NN	denotes	E2
T16520	6892-6899	NN	denotes	protein
T16519	6883-6888	JJ	denotes	human
T16518	6880-6882	IN	denotes	of
T16517	6869-6879	NN	denotes	production
T16516	6865-6868	CC	denotes	and
T16515	6849-6855	NN	denotes	enzyme
T16514	6856-6864	NN	denotes	activity
T16513	6843-6848	NN	denotes	BCKDH
T16512	6841-6843	,	denotes	,
T16511	6840-6841	-RRB-	denotes	]
T16510	6838-6840	CD	denotes	34
T16509	6837-6838	-LRB-	denotes	[
T16508	6826-6836	NN	denotes	expression
T16507	6817-6825	JJ	denotes	specific
T16506	6811-6816	NN	denotes	liver
T16505	6803-6810	VB	denotes	produce
T16504	6800-6802	TO	denotes	to
T16503	6933-6941	VBN	denotes	examined
T16502	6782-6786	VBN	denotes	been
T16482	6047-6048	sentence	denotes
T16481	6046-6047	.	denotes	.
T16480	6045-6046	-RRB-	denotes	)
T16479	6044-6045	CD	denotes	2
T16478	6038-6043	NN	denotes	Table
T16477	6037-6038	-LRB-	denotes	(
T16476	6028-6036	NNS	denotes	controls
T16475	6025-6027	IN	denotes	to
T16474	6016-6024	VBN	denotes	compared
T16473	6014-6016	,	denotes	,
T17014	9300-9304	RB	denotes	very
T17013	9312-9317	NN	denotes	model
T17012	9298-9299	DT	denotes	a
T17011	9276-9282	NN	denotes	rescue
T17010	9283-9287	NNS	denotes	mice
T17009	9270-9275	DT	denotes	these
T17008	9268-9270	,	denotes	,
T17007	9288-9297	VBP	denotes	represent
T17006	9264-9268	RB	denotes	Thus
T17005	9263-9353	sentence	denotes	Thus, these rescue mice represent a very useful model of the intermediate MSUD phenotype.
T17004	9262-9263	.	denotes	.
T17003	9261-9262	-RRB-	denotes	]
T17002	9260-9261	CD	denotes	1
T17001	9259-9260	-LRB-	denotes	[
T17000	9251-9258	NN	denotes	disease
T16999	9247-9250	DT	denotes	the
T16998	9244-9246	IN	denotes	of
T16997	9226-9238	JJ	denotes	intermediate
T16996	9239-9243	NN	denotes	form
T16995	9222-9225	DT	denotes	the
T16994	9217-9221	IN	denotes	with
T16993	9208-9216	NNS	denotes	patients
T16992	9203-9207	NN	denotes	MSUD
T16991	9200-9202	IN	denotes	in
T16990	9191-9199	VBN	denotes	observed
T16989	9172-9180	JJ	denotes	clinical
T16988	9181-9190	NN	denotes	phenotype
T16987	9168-9171	DT	denotes	the
T16986	9165-9167	IN	denotes	to
T16985	9157-9164	JJ	denotes	similar
T16984	9146-9156	RB	denotes	remarkably
T16983	9142-9145	VBP	denotes	are
T16982	9118-9128	JJ	denotes	phenotypic
T16981	9129-9141	NNS	denotes	observations
T16980	9112-9117	DT	denotes	These
T16979	9111-9263	sentence	denotes	These phenotypic observations are remarkably similar to the clinical phenotype observed in MSUD patients with the intermediate form of the disease [1].
T16978	9110-9111	.	denotes	.
T16977	9105-9110	NN	denotes	liver
T16976	9101-9104	DT	denotes	the
T16975	9098-9100	IN	denotes	in
T16974	9082-9088	NN	denotes	enzyme
T16973	9076-9081	NN	denotes	BCKDH
T16972	9089-9097	NN	denotes	activity
T16971	9069-9075	JJ	denotes	normal
T16970	9066-9068	IN	denotes	of
T16969	9064-9065	NN	denotes	%
T16968	9062-9063	SYM	denotes	–
T16967	9061-9062	CD	denotes	5
T16966	9063-9064	CD	denotes	6
T16965	9060-9061	SYM	denotes	~
T16964	9050-9059	VBD	denotes	expressed
T16963	9045-9049	PRP	denotes	they
T16962	9041-9044	CC	denotes	and
T16961	9031-9040	NNS	denotes	knockouts
T16960	9027-9030	CC	denotes	and
T16959	9018-9026	NNS	denotes	controls
T16958	9010-9017	IN	denotes	between
T16957	8997-9009	JJ	denotes	intermediate
T16956	8992-8996	VBD	denotes	were
T16955	8987-8991	WDT	denotes	that
T16954	8980-8986	NNS	denotes	ratios
T16953	8971-8972	HYPH	denotes	/
T16952	8972-8979	NN	denotes	alanine
T16951	8967-8971	NN	denotes	BCAA
T16950	8948-8957	VBG	denotes	surviving
T16949	8958-8962	NNS	denotes	mice
T16948	8942-8947	DT	denotes	these
T16947	8940-8942	,	denotes	,
T16946	8933-8940	NN	denotes	summary
T16945	8963-8966	VBD	denotes	had
T16944	8930-8932	IN	denotes	In
T16943	8929-9111	sentence	denotes	In summary, these surviving mice had BCAA/alanine ratios that were intermediate between controls and knockouts and they expressed ~5–6% of normal BCKDH enzyme activity in the liver.
T16942	8928-8929	.	denotes	.
T16941	8925-8928	NN	denotes	age
T16940	8922-8924	IN	denotes	of
T16939	8913-8915	CD	denotes	60
T16938	8909-8912	CC	denotes	and
T16937	8916-8921	NNS	denotes	weeks
T16936	8906-8908	CD	denotes	40
T16935	8903-8905	IN	denotes	at
T16934	8898-8902	VBD	denotes	died
T16933	8883-8887	JJ	denotes	rare
T16932	8879-8882	CD	denotes	two
T16931	8888-8897	NNS	denotes	survivors
T16930	8873-8878	DT	denotes	These
T16929	8872-8929	sentence	denotes	These two rare survivors died at 40 and 60 weeks of age.
T16928	8871-8872	.	denotes	.
T16927	8866-8871	JJ	denotes	alive
T16926	8860-8865	RB	denotes	still
T16925	8853-8854	-RRB-	denotes	)
T16924	8851-8853	CD	denotes	16
T16923	8848-8850	IN	denotes	of
T16922	8846-8847	CD	denotes	2
T16921	8845-8846	-LRB-	denotes	(
T16920	8840-8844	NNS	denotes	mice
T16919	8837-8839	IN	denotes	of
T16918	8835-8836	NN	denotes	%
T16917	8833-8835	CD	denotes	12
T16916	8832-8833	SYM	denotes	~
T16915	8830-8832	,	denotes	,
T16914	8827-8830	NN	denotes	age
T16913	8824-8826	IN	denotes	of
T16912	8818-8823	NNS	denotes	weeks
T16911	8815-8817	CD	denotes	20
T16910	8855-8859	VBD	denotes	were
T16909	8812-8814	IN	denotes	At
T16908	8811-8872	sentence	denotes	At 20 weeks of age, ~12% of mice (2 of 16) were still alive.
T16907	8810-8811	.	denotes	.
T16906	8804-8810	JJR	denotes	better
T16905	8795-8803	RB	denotes	somewhat
T16904	8792-8794	VB	denotes	be
T16903	8789-8791	TO	denotes	to
T16902	8775-8779	NN	denotes	525A
T16901	8770-8774	NN	denotes	Line
T16900	8767-8769	IN	denotes	of
T16899	8780-8788	VBD	denotes	appeared
T16898	8758-8766	NN	denotes	Survival
T16897	8757-8811	sentence	denotes	Survival of Line 525A appeared to be somewhat better.
T16896	8756-8757	.	denotes	.
T16895	8752-8756	NN	denotes	cage
T16894	8746-8751	PRP$	denotes	their
T16893	8743-8745	IN	denotes	in
T16892	8738-8742	JJ	denotes	dead
T16891	8732-8737	VBN	denotes	found
T16890	8727-8731	VBD	denotes	were
T16889	8724-8726	CC	denotes	or
T16888	8722-8724	,	denotes	,
T16887	8712-8722	VBN	denotes	sacrificed
T16886	8703-8711	RB	denotes	humanely
T16885	8699-8702	CC	denotes	and
T16884	8690-8698	JJ	denotes	moribund
T16883	8683-8684	NN	denotes	A
T16882	8678-8682	NN	denotes	Line
T16881	8675-8677	IN	denotes	of
T16880	8670-8674	NNS	denotes	mice
T16879	8666-8669	DT	denotes	all
T16878	8664-8666	,	denotes	,
T16877	8659-8664	NNS	denotes	weeks
T16876	8656-8658	CD	denotes	16
T16875	8685-8689	VBD	denotes	were
T16874	8653-8655	IN	denotes	By
T16873	8652-8757	sentence	denotes	By 16 weeks, all mice of Line A were moribund and humanely sacrificed, or were found dead in their cage.
T16872	8651-8652	.	denotes	.
T16871	8636-8639	VBD	denotes	was
T16870	8624-8630	NN	denotes	rescue
T16869	8631-8635	NNS	denotes	mice
T16868	8620-8623	DT	denotes	the
T16867	8617-8619	IN	denotes	of
T16866	8608-8616	NN	denotes	survival
T16865	8640-8651	VBN	denotes	compromised
T16864	8603-8607	IN	denotes	that
T16863	8594-8602	JJ	denotes	apparent
T16862	8586-8593	RB	denotes	readily
T16861	8580-8582	PRP	denotes	it
T16860	8578-8580	,	denotes	,
T16859	8583-8585	VBZ	denotes	is
T16858	8565-8568	RB	denotes	not
T16857	8561-8564	VBD	denotes	was
T16856	8553-8560	NNS	denotes	animals
T16855	8545-8552	NN	denotes	control
T16854	8541-8544	IN	denotes	for
T16853	8536-8540	NNS	denotes	data
T16852	8527-8535	NN	denotes	survival
T16851	8569-8578	VBN	denotes	collected
T16850	8518-8526	IN	denotes	Although
T16849	8517-8652	sentence	denotes	Although survival data for control animals was not collected, it is readily apparent that survival of the rescue mice was compromised.
T16848	8516-8517	.	denotes	.
T16847	8514-8516	NN	denotes	4D
T16846	8509-8513	NN	denotes	Fig.
T16845	8506-8508	IN	denotes	in
T16844	8495-8497	VBZ	denotes	is
T16843	8487-8494	NN	denotes	weaning
T16842	8480-8486	IN	denotes	beyond
T16841	8471-8479	VBD	denotes	survived
T16840	8466-8470	WDT	denotes	that
T16839	8449-8459	JJ	denotes	transgenic
T16838	8445-8448	CD	denotes	two
T16837	8460-8465	NNS	denotes	lines
T16836	8439-8444	DT	denotes	these
T16835	8434-8438	IN	denotes	from
T16834	8429-8433	NNS	denotes	mice
T16833	8425-8428	DT	denotes	the
T16832	8422-8424	IN	denotes	of
T16831	8498-8505	VBN	denotes	plotted
T16830	8413-8421	NN	denotes	survival
T16829	8407-8408	HYPH	denotes	-
T16828	8408-8412	NN	denotes	term
T16827	8403-8407	JJ	denotes	Long
T16826	8402-8517	sentence	denotes	Long-term survival of the mice from these two transgenic lines that survived beyond weaning is plotted in Fig. 4D.
T16472	6010-6014	NNS	denotes	mice
T16471	6005-6009	NN	denotes	525A
T16470	6000-6004	NN	denotes	Line
T16469	5997-5999	IN	denotes	in
T16468	5987-5996	NN	denotes	glutamate
T16467	5983-5986	VBD	denotes	was
T16466	5980-5982	IN	denotes	as
T16465	5978-5980	,	denotes	,
T16464	5974-5978	NNS	denotes	mice
T16463	5972-5973	NN	denotes	A
T16462	5967-5971	NN	denotes	Line
T16461	5964-5966	IN	denotes	in
T16460	5951-5955	VBD	denotes	were
T16459	5941-5950	NN	denotes	glutamine
T16458	5937-5940	CC	denotes	and
T16457	5935-5937	,	denotes	,
T16456	5926-5935	NN	denotes	glutamate
T16455	5924-5926	,	denotes	,
T16454	5917-5924	NN	denotes	alanine
T16453	5914-5916	IN	denotes	of
T16452	5956-5963	VBN	denotes	reduced
T16451	5907-5913	NNS	denotes	levels
T16450	5901-5906	NN	denotes	Blood
T16449	5900-6047	sentence	denotes	Blood levels of alanine, glutamate, and glutamine were reduced in Line A mice, as was glutamate in Line 525A mice, compared to controls (Table 2).
T16448	5899-5900	.	denotes	.
T16447	5898-5899	-RRB-	denotes	)
T16446	5896-5898	NN	denotes	2C
T16445	5889-5895	NN	denotes	Figure
T16444	5885-5888	VB	denotes	see
T16443	5884-5885	-LRB-	denotes	(
T16442	5874-5883	NNS	denotes	knockouts
T16441	5870-5873	CC	denotes	and
T16440	5861-5869	NNS	denotes	controls
T16439	5853-5860	IN	denotes	between
T16438	5840-5852	JJ	denotes	intermediate
T16437	5820-5830	JJ	denotes	transgenic
T16436	5831-5835	NNS	denotes	mice
T16435	5816-5819	DT	denotes	the
T16434	5812-5815	IN	denotes	for
T16433	5796-5797	HYPH	denotes	/
T16432	5797-5804	NN	denotes	alanine
T16431	5792-5796	NN	denotes	BCAA
T16430	5805-5811	NNS	denotes	values
T16429	5788-5791	DT	denotes	the
T16428	5836-5839	VBP	denotes	are
T16427	5783-5787	IN	denotes	that
T16426	5778-5782	VB	denotes	Note
T16425	5777-5900	sentence	denotes	Note that the BCAA/alanine values for the transgenic mice are intermediate between controls and knockouts (see Figure 2C).
T16424	5776-5777	.	denotes	.
T16423	5768-5776	VBN	denotes	analyzed
T16422	5756-5760	NN	denotes	time
T16421	5761-5767	NNS	denotes	points
T16420	5752-5755	DT	denotes	the
T16419	5749-5751	IN	denotes	of
T16418	5745-5748	DT	denotes	all
T16417	5741-5744	IN	denotes	for
T16416	5732-5740	NNS	denotes	controls
T16415	5729-5731	IN	denotes	to
T16414	5720-5728	VBN	denotes	compared
T16413	5711-5719	JJ	denotes	elevated
T16412	5697-5710	RB	denotes	significantly
T16411	5686-5691	NNS	denotes	lines
T16410	5681-5685	DT	denotes	both
T16409	5676-5680	IN	denotes	from
T16408	5671-5675	NNS	denotes	mice
T16407	5667-5670	IN	denotes	for
T16406	5692-5696	VBD	denotes	were
T16405	5651-5652	HYPH	denotes	/
T16404	5652-5659	NN	denotes	alanine
T16403	5647-5651	NN	denotes	BCAA
T16402	5660-5666	NNS	denotes	values
T16401	5643-5646	DT	denotes	The
T16400	5642-5777	sentence	denotes	The BCAA/alanine values for mice from both lines were significantly elevated compared to controls for all of the time points analyzed.
T16399	5641-5642	.	denotes	.
T16398	5634-5641	NN	denotes	alanine
T16397	5631-5633	IN	denotes	to
T16396	5626-5630	NN	denotes	BCAA
T16395	5620-5625	JJ	denotes	total
T16394	5617-5619	IN	denotes	of
T16393	5611-5616	NN	denotes	ratio
T16392	5609-5610	DT	denotes	a
T16391	5606-5608	IN	denotes	as
T16390	5596-5605	VBN	denotes	presented
T16389	5582-5586	NN	denotes	acid
T16388	5576-5581	NN	denotes	amino
T16387	5587-5595	NN	denotes	analysis
T16386	5570-5575	NN	denotes	blood
T16385	5567-5569	IN	denotes	of
T16384	5559-5566	NNS	denotes	results
T16383	5555-5558	DT	denotes	the
T16337	5479-5480	sentence	denotes	L
T16336	4310-4311	sentence	denotes
T16335	4309-4310	.	denotes	.
T16334	4305-4309	NNS	denotes	pups
T16333	4298-4304	VBN	denotes	weaned
T16332	4295-4297	IN	denotes	of
T16331	4292-4293	CD	denotes	5
T16330	4288-4291	CC	denotes	and
T16329	4293-4294	NN	denotes	%
T16328	4286-4287	CD	denotes	1
T16327	4278-4285	IN	denotes	between
T16326	4268-4277	NN	denotes	frequency
T16325	4266-4267	DT	denotes	a
T16324	4263-4265	IN	denotes	at
T16323	4248-4254	NN	denotes	rescue
T16322	4255-4262	NNS	denotes	animals
T16277	4004-4005	.	denotes	.
T16276	3989-3995	NN	denotes	rescue
T16275	3996-4004	NN	denotes	genotype
T16274	3985-3988	DT	denotes	the
T16273	3976-3980	NN	denotes	line
T16272	3971-3975	DT	denotes	this
T16271	3966-3970	IN	denotes	from
T16270	3951-3960	VBG	denotes	surviving
T16269	3949-3951	,	denotes	,
T16268	3961-3965	NNS	denotes	pups
T16267	3943-3949	VBN	denotes	weaned
T16266	3940-3942	IN	denotes	of
T16265	3981-3984	VBD	denotes	had
T16264	3938-3939	NN	denotes	%
T16263	3936-3938	CD	denotes	10
T16262	3922-3935	RB	denotes	Approximately
T16261	3921-4005	sentence	denotes	Approximately 10% of weaned, surviving pups from this line had the rescue genotype.
T16260	3920-3921	.	denotes	.
T16259	3915-3918	CD	denotes	525
T16258	3919-3920	NN	denotes	A
T16257	3910-3914	NN	denotes	line
T16256	3898-3905	NN	denotes	weaning
T16255	3895-3897	IN	denotes	at
T16254	3890-3894	NNS	denotes	mice
T16253	3883-3889	NN	denotes	rescue
T16252	3880-3882	IN	denotes	of
T16251	3861-3868	JJS	denotes	highest
T16250	3869-3879	NN	denotes	percentage
T16249	3857-3860	DT	denotes	the
T16248	3852-3856	IN	denotes	with
T16247	3906-3909	VBD	denotes	was
T16246	3847-3851	NN	denotes	line
T16245	3843-3846	DT	denotes	The
T16244	3842-3921	sentence	denotes	The line with the highest percentage of rescue mice at weaning was line 525 A.
T16243	3841-3842	.	denotes	.
T16242	3836-3841	NNS	denotes	lines
T16241	3828-3835	IN	denotes	between
T16240	3815-3818	VBD	denotes	was
T16239	3819-3827	VBN	denotes	observed
T16238	3803-3814	NN	denotes	variability
T16237	3790-3802	JJ	denotes	Considerable
T16236	3789-3842	sentence	denotes	Considerable variability was observed between lines.
T16235	3788-3789	.	denotes	.
T16234	3782-3788	VBN	denotes	tested
T16233	3766-3776	JJ	denotes	transgenic
T16232	3777-3781	NN	denotes	line
T16231	3761-3765	DT	denotes	each
T16230	3756-3760	IN	denotes	from
T16229	3740-3746	NN	denotes	rescue
T16220	3695-3705	NN	denotes	percentage
T17021	9352-9353	.	denotes	.
T17020	9338-9342	NN	denotes	MSUD
T17019	9325-9337	JJ	denotes	intermediate
T17018	9343-9352	NN	denotes	phenotype
T17017	9321-9324	DT	denotes	the
T17016	9318-9320	IN	denotes	of
T16609	7293-7295	IN	denotes	to
T16608	7287-7292	JJ	denotes	equal
T16607	7273-7286	RB	denotes	approximately
T16606	7266-7268	NN	denotes	E2
T16605	7260-7265	JJ	denotes	human
T16604	7257-7259	IN	denotes	of
T16603	7251-7256	NN	denotes	level
T16602	7247-7250	DT	denotes	the
T16601	7245-7247	,	denotes	,
T16600	7230-7240	JJ	denotes	transgenic
T16599	7241-7245	NNS	denotes	mice
T16598	7224-7229	DT	denotes	these
T16597	7221-7223	IN	denotes	of
T16596	7216-7220	DT	denotes	some
T16595	7269-7272	VBD	denotes	was
T16594	7213-7215	IN	denotes	In
T16593	7212-7376	sentence	denotes	In some of these transgenic mice, the level of human E2 was approximately equal to the amount of mouse E2 (~47 Kd) produced in liver of nontransgenic control mice.
T16592	7211-7212	.	denotes	.
T16591	7207-7211	NNS	denotes	mice
T16590	7199-7206	IN	denotes	between
T16589	7190-7198	JJ	denotes	variable
T16588	7184-7189	RB	denotes	quite
T16587	7164-7174	JJ	denotes	transgenic
T16586	7175-7179	NNS	denotes	mice
T16585	7158-7163	DT	denotes	these
T16584	7155-7157	IN	denotes	in
T16583	7153-7154	-RRB-	denotes	)
T16582	7148-7150	NN	denotes	54
T16581	7147-7148	SYM	denotes	~
T16580	7145-7147	NN	denotes	MW
T16579	7135-7144	VBN	denotes	predicted
T16578	7151-7153	NN	denotes	Kd
T16577	7134-7135	-LRB-	denotes	(
T16576	7131-7133	NN	denotes	E2
T16575	7125-7130	JJ	denotes	human
T16574	7122-7124	IN	denotes	of
T16573	7115-7121	NN	denotes	amount
T16572	7111-7114	DT	denotes	the
T16571	7109-7111	,	denotes	,
T16570	7107-7109	NN	denotes	3B
T16569	7102-7106	NNP	denotes	Fig.
T16568	7099-7101	IN	denotes	in
T16567	7180-7183	VBD	denotes	was
T16566	7093-7098	VBN	denotes	shown
T16565	7090-7092	IN	denotes	As
T16564	7089-7212	sentence	denotes	As shown in Fig. 3B, the amount of human E2 (predicted MW~54 Kd) in these transgenic mice was quite variable between mice.
T16563	7088-7089	.	denotes	.
T16562	7087-7088	-RRB-	denotes	)
T16561	7080-7084	NN	denotes	Fig.
T16560	7085-7087	NN	denotes	4C
T16559	7079-7080	-LRB-	denotes	(
T16558	7073-7078	NN	denotes	liver
T16557	7065-7072	NN	denotes	control
T16556	7062-7064	IN	denotes	in
T16555	7054-7061	JJ	denotes	present
T16554	7035-7044	JJ	denotes	enzymatic
T16553	7045-7053	NN	denotes	activity
T16552	7031-7034	DT	denotes	the
T16551	7028-7030	IN	denotes	of
T16550	7026-7028	,	denotes	,
T16549	7014-7026	RB	denotes	respectively
T16548	7012-7014	,	denotes	,
T16547	7010-7011	CD	denotes	5
T16546	7006-7009	CC	denotes	and
T16545	7011-7012	NN	denotes	%
T16544	7004-7005	CD	denotes	6
T16543	7003-7004	SYM	denotes	~
T16542	6994-6998	NN	denotes	525A
T16541	6990-6993	CC	denotes	and
T16540	6988-6989	NN	denotes	A
T16539	6982-6987	NNS	denotes	Lines
T16538	6977-6981	IN	denotes	from
T16537	6971-6976	NN	denotes	liver
T16536	6968-6970	IN	denotes	in
T16535	6999-7002	VBD	denotes	was
T16534	6949-6958	JJ	denotes	enzymatic
T16533	6959-6967	NN	denotes	activity
T16532	6943-6948	NN	denotes	BCKDH
T16531	6942-7089	sentence	denotes	BCKDH enzymatic activity in liver from Lines A and 525A was ~6 and 5%, respectively, of the enzymatic activity present in control liver (Fig. 4C).
T16530	6941-6942	.	denotes	.
T16228	3747-3755	NN	denotes	genotype
T16227	3736-3739	DT	denotes	the
T16226	3731-3735	IN	denotes	with
T16225	3723-3730	NN	denotes	weaning
T16224	3720-3722	IN	denotes	at
T16223	3714-3719	JJ	denotes	alive
T16222	3709-3713	NNS	denotes	mice
T16221	3706-3708	IN	denotes	of
T16219	3691-3694	DT	denotes	the
T16218	3685-3690	VBZ	denotes	shows
T16217	3682-3684	NN	denotes	4A
T16216	3677-3681	NN	denotes	Fig.
T16215	3676-3789	sentence	denotes	Fig. 4A shows the percentage of mice alive at weaning with the rescue genotype from each transgenic line tested.
T16214	3675-3676	.	denotes	.
T16213	3674-3675	NN	denotes	%
T16212	3672-3674	CD	denotes	14
T16211	3669-3671	IN	denotes	of
T16210	3667-3668	-RRB-	denotes	)
T16209	3657-3667	NNS	denotes	transgenes
T16208	3652-3656	DT	denotes	both
T16207	3648-3651	IN	denotes	for
T16206	3639-3647	JJ	denotes	positive
T16205	3635-3638	CC	denotes	and
T16204	3623-3625	NN	denotes	E2
T16203	3612-3622	JJ	denotes	homozygous
T16202	3610-3612	,	denotes	,
T16201	3606-3610	FW	denotes	i.e.
T16200	3626-3634	NN	denotes	knockout
T16199	3605-3606	-LRB-	denotes	(
T16198	3589-3595	NN	denotes	rescue
T16197	3596-3604	NN	denotes	genotype
T16196	3585-3588	DT	denotes	the
T16195	3580-3584	IN	denotes	with
T16194	3572-3579	NNS	denotes	animals
T16193	3569-3571	IN	denotes	of
T16192	3549-3560	JJ	denotes	theoretical
T16191	3561-3568	NN	denotes	maximum
T16190	3547-3548	DT	denotes	a
T16189	3544-3546	IN	denotes	in
T16188	3537-3543	VB	denotes	result
T16187	3534-3536	TO	denotes	to
T16186	3522-3524	VBZ	denotes	is
T16185	3525-3533	VBN	denotes	expected
T16184	3504-3512	VBG	denotes	breeding
T16183	3513-3521	NN	denotes	strategy
T16182	3499-3503	DT	denotes	This
T16181	3498-3676	sentence	denotes	This breeding strategy is expected to result in a theoretical maximum of animals with the rescue genotype (i.e., homozygous E2 knockout and positive for both transgenes) of 14%.
T16180	3497-3498	.	denotes	.
T16179	3490-3497	NN	denotes	weaning
T16178	3487-3489	IN	denotes	at
T16177	3479-3486	NNS	denotes	litters
T16176	3466-3468	PRP	denotes	we
T16175	3464-3466	,	denotes	,
T16174	3455-3464	NN	denotes	lethality
T16173	3446-3454	JJ	denotes	neonatal
T16172	3441-3445	IN	denotes	from
T16171	3432-3440	NN	denotes	knockout
T16170	3428-3431	DT	denotes	the
T16169	3421-3427	VB	denotes	rescue
T16168	3418-3420	TO	denotes	to
T16167	3407-3417	NNS	denotes	transgenes
T16166	3403-3406	DT	denotes	the
T16165	3400-3402	IN	denotes	of
T16164	3392-3399	NN	denotes	ability
T16163	3388-3391	DT	denotes	the
T16162	3384-3387	IN	denotes	for
T16161	3469-3478	VBD	denotes	genotyped
T16160	3365-3376	RB	denotes	efficiently
T16159	3377-3383	VB	denotes	screen
T16158	3362-3364	TO	denotes	To
T16157	3361-3498	sentence	denotes	To efficiently screen for the ability of the transgenes to rescue the knockout from neonatal lethality, we genotyped litters at weaning.
T16156	3360-3361	.	denotes	.
T16155	3349-3351	NN	denotes	E2
T16154	3352-3360	NN	denotes	knockout
T16153	3345-3348	DT	denotes	the
T16152	3341-3344	CC	denotes	and
T16151	3339-3341	,	denotes	,
T16150	3326-3327	HYPH	denotes	-
T16149	3327-3329	NN	denotes	E2
T16148	3323-3326	NN	denotes	TRE
T16147	3330-3339	NN	denotes	transgene
T16146	3319-3322	DT	denotes	the
T16145	3317-3319	,	denotes	,
T16144	3303-3304	HYPH	denotes	-
T16143	3304-3307	NN	denotes	tTA
T16142	3300-3303	NN	denotes	LAP
T16141	3308-3317	NN	denotes	transgene
T16140	3296-3299	DT	denotes	the
T16139	3292-3295	IN	denotes	for
T16138	3279-3291	JJ	denotes	heterozygous
T16137	3269-3273	NNS	denotes	mice
T16136	3265-3268	DT	denotes	the
T16135	3259-3264	WDT	denotes	which
T16134	3274-3278	VBD	denotes	were
T16133	3256-3258	IN	denotes	in
T16132	3246-3250	VBD	denotes	were
T16131	3240-3245	NNS	denotes	pairs
T16130	3231-3239	VBG	denotes	breeding
T16129	3229-3231	,	denotes	,
T16128	3251-3255	VBN	denotes	used
T16127	3219-3229	RB	denotes	Ultimately
T16126	3218-3361	sentence	denotes	Ultimately, breeding pairs were used in which the mice were heterozygous for the LAP-tTA transgene, the TRE-E2 transgene, and the E2 knockout.
T16125	3217-3218	.	denotes	.
T16124	3206-3208	NN	denotes	E2
T16123	3209-3217	NN	denotes	knockout
T16122	3202-3205	DT	denotes	the
T16121	3198-3201	IN	denotes	for
T16120	3185-3197	JJ	denotes	heterozygous
T16119	3180-3184	VBD	denotes	were
T16118	3176-3179	CC	denotes	and
T16117	3161-3162	HYPH	denotes	-
T16116	3162-3165	NN	denotes	tTA
T16115	3158-3161	NN	denotes	LAP
T16114	3166-3175	NN	denotes	transgene
T16113	3154-3157	DT	denotes	the
T16112	3150-3153	IN	denotes	for
T16111	3141-3149	JJ	denotes	positive
T16110	3136-3140	VBD	denotes	were
T16109	3131-3135	WDT	denotes	that
T16108	3126-3130	NNS	denotes	mice
T16107	3123-3125	IN	denotes	to
T16106	3110-3114	VBD	denotes	were
T16105	3108-3109	-RRB-	denotes	)
T16104	3096-3098	NN	denotes	F1
T16103	3093-3095	CC	denotes	or
T16102	3084-3092	NNS	denotes	founders
T16101	3077-3083	CC	denotes	either
T16100	3099-3108	NN	denotes	offspring
T16099	3076-3077	-LRB-	denotes	(
T16098	3071-3075	NN	denotes	line
T16097	3066-3070	DT	denotes	each
T16096	3061-3065	IN	denotes	from
T16095	3115-3122	VBN	denotes	crossed
T16094	3052-3053	HYPH	denotes	-
T16093	3053-3055	NN	denotes	E2
T16092	3049-3052	NN	denotes	TRE
T16091	3056-3060	NNS	denotes	mice
T16090	3038-3048	JJ	denotes	Transgenic
T16089	3037-3218	sentence	denotes	Transgenic TRE-E2 mice from each line (either founders or F1 offspring) were crossed to mice that were positive for the LAP-tTA transgene and were heterozygous for the E2 knockout.
T16088	3036-3037	.	denotes	.
T16087	3035-3036	-RRB-	denotes	)
T16086	3026-3029	RB	denotes	not
T16085	3021-3025	NNS	denotes	data
T16084	3030-3035	VBN	denotes	shown
T16083	3020-3021	-LRB-	denotes	(
T16082	3016-3019	NN	denotes	DNA
T16081	3011-3015	NN	denotes	tail
T16080	3008-3010	IN	denotes	of
T16079	2999-3007	NN	denotes	analysis
T16078	2994-2998	NN	denotes	blot
T16077	2985-2993	NNP	denotes	Southern
T16076	2982-2984	IN	denotes	by
T16075	2973-2981	VBN	denotes	compared
T16074	2970-2972	IN	denotes	as
T16073	2958-2962	NN	denotes	copy
T16072	2963-2969	NN	denotes	number
T16071	2948-2957	NN	denotes	transgene
T16070	2945-2947	IN	denotes	in
T15888	848-855	JJ	denotes	minimal
T15887	837-847	VBG	denotes	containing
T15886	833-836	NN	denotes	TRE
T15885	831-832	DT	denotes	a
T15884	826-830	IN	denotes	from
T15883	818-820	NN	denotes	E2
T15882	812-817	JJ	denotes	human
T15881	821-825	NN	denotes	cDNA
T15880	810-811	DT	denotes	a
T15879	802-809	VB	denotes	express
T15878	799-801	TO	denotes	to
T15877	786-789	VBD	denotes	was
T15876	790-798	VBN	denotes	designed
T15875	772-773	HYPH	denotes	-
T15874	773-775	NN	denotes	E2
T15873	769-772	NN	denotes	TRE
T15872	776-785	NN	denotes	transgene
T15871	765-768	DT	denotes	The
T15870	764-889	sentence	denotes	The TRE-E2 transgene was designed to express a human E2 cDNA from a TRE containing minimal promoter upon stimulation by tTA.
T15869	763-764	.	denotes	.
T15868	762-763	-RRB-	denotes	)
T15867	759-762	NN	denotes	TRE
T15866	758-759	-LRB-	denotes	(
T15865	741-749	NN	denotes	response
T15864	726-740	NN	denotes	transactivator
T15863	750-757	NN	denotes	element
T15862	724-725	DT	denotes	a
T15861	717-723	VBP	denotes	harbor
T15860	712-716	WDT	denotes	that
T15859	702-711	NNS	denotes	promoters
T15858	699-701	IN	denotes	of
T15857	688-698	NN	denotes	expression
T15856	677-687	VBZ	denotes	stimulates
T15855	672-676	WDT	denotes	that
T15854	651-664	NN	denotes	transcription
T15853	665-671	NN	denotes	factor
T15852	649-650	DT	denotes	a
T15851	647-649	,	denotes	,
T15850	646-647	-RRB-	denotes	)
T15849	643-646	NN	denotes	tTA
T15848	642-643	-LRB-	denotes	(
T15847	615-616	HYPH	denotes	-
T15846	616-626	VBN	denotes	controlled
T15845	603-615	NN	denotes	tetracycline
T15844	627-641	NN	denotes	transactivator
T15843	599-602	DT	denotes	the
T15842	596-598	IN	denotes	of
T15841	585-595	NN	denotes	expression
T15840	576-584	JJ	denotes	specific
T15839	570-575	NN	denotes	liver
T15838	567-569	IN	denotes	of
T15837	560-566	NNS	denotes	levels
T15836	555-559	JJ	denotes	high
T15835	545-546	-RRB-	denotes	]
T15834	543-545	CD	denotes	34
T15833	542-543	-LRB-	denotes	[
T15832	547-554	VBZ	denotes	directs
T15831	527-528	HYPH	denotes	-
T15830	528-531	NN	denotes	tTA
T15829	524-527	NN	denotes	LAP
T15828	532-541	NN	denotes	transgene
T15827	520-523	DT	denotes	The
T15826	519-764	sentence	denotes	The LAP-tTA transgene [34] directs high levels of liver specific expression of the tetracycline-controlled transactivator (tTA), a transcription factor that stimulates expression of promoters that harbor a transactivator response element (TRE).
T15825	518-519	.	denotes	.
T15824	517-518	-RRB-	denotes	)
T15823	510-514	NN	denotes	Fig.
T15822	515-517	NN	denotes	3A
T15821	509-510	-LRB-	denotes	(
T15820	495-496	HYPH	denotes	-
T15819	496-498	NN	denotes	E2
T15818	492-495	NN	denotes	TRE
T15817	499-508	NN	denotes	transgene
T15816	490-491	DT	denotes	a
T15815	486-489	CC	denotes	and
T15814	471-472	HYPH	denotes	-
T15813	472-475	NN	denotes	tTA
T15812	468-471	NN	denotes	LAP
T15811	476-485	NN	denotes	transgene
T15810	466-467	DT	denotes	a
T15809	463-465	IN	denotes	of
T15808	453-462	VBD	denotes	consisted
T15807	432-445	JJ	denotes	bi-transgenic
T15806	446-452	NN	denotes	system
T15805	427-431	DT	denotes	This
T15804	426-519	sentence	denotes	This bi-transgenic system consisted of a LAP-tTA transgene and a TRE-E2 transgene (Fig. 3A).
T15803	425-426	.	denotes	.
T15802	406-414	NN	denotes	knockout
T15801	403-405	NN	denotes	E2
T15800	415-425	NN	denotes	background
T15799	399-402	DT	denotes	the
T15798	396-398	IN	denotes	on
T15797	390-395	NN	denotes	liver
T15796	387-389	IN	denotes	in
T15795	384-386	NN	denotes	E2
T15794	378-383	JJ	denotes	human
T15793	370-377	VB	denotes	express
T15792	367-369	TO	denotes	to
T15791	349-359	JJ	denotes	transgenic
T15790	344-348	NN	denotes	part
T15789	340-343	CD	denotes	two
T15788	360-366	NN	denotes	system
T15787	338-339	DT	denotes	a
T15786	335-337	IN	denotes	of
T15785	322-325	VBD	denotes	was
T15784	326-334	VBN	denotes	composed
T15783	313-321	NN	denotes	strategy
T15782	309-312	PRP$	denotes	Our
T15781	308-426	sentence	denotes	Our strategy was composed of a two part transgenic system to express human E2 in liver on the E2 knockout background.
T15780	307-308	.	denotes	.
T15779	296-301	NN	denotes	mouse
T15778	291-295	NN	denotes	MSUD
T15777	283-290	JJ	denotes	classic
T15776	302-307	NN	denotes	model
T15775	279-282	DT	denotes	the
T15774	276-278	IN	denotes	in
T15773	269-275	VBZ	denotes	occurs
T15772	264-268	WDT	denotes	that
T15771	254-263	NN	denotes	lethality
T15770	245-253	JJ	denotes	neonatal
T15769	241-244	CC	denotes	and
T15768	236-240	NN	denotes	BCAA
T15767	233-235	IN	denotes	of
T15766	216-222	JJ	denotes	severe
T15765	223-232	NN	denotes	elevation
T15764	212-215	DT	denotes	the
T15763	205-211	VB	denotes	rescue
T15762	202-204	TO	denotes	to
T15761	182-192	JJ	denotes	transgenic
T15760	193-201	NN	denotes	strategy
T15759	180-181	DT	denotes	a
T15758	172-174	PRP	denotes	we
T15757	170-172	,	denotes	,
T15756	166-170	NN	denotes	MSUD
T15755	163-165	IN	denotes	of
T15754	150-157	NN	denotes	variant
T15753	137-149	JJ	denotes	intermediate
T15752	158-162	NN	denotes	form
T15751	133-136	DT	denotes	the
T15750	130-132	IN	denotes	of
T15749	117-123	JJ	denotes	murine
T15748	124-129	NN	denotes	model
T15747	115-116	DT	denotes	a
T15746	175-179	VBD	denotes	used
T15745	108-114	VB	denotes	create
T15744	105-107	TO	denotes	To
T15743	104-308	sentence	denotes	To create a murine model of the intermediate variant form of MSUD, we used a transgenic strategy to rescue the severe elevation of BCAA and neonatal lethality that occurs in the classic MSUD mouse model.
T15742	100-104	NN	denotes	MSUD
T15741	87-99	JJ	denotes	intermediate
T15740	80-86	VBZ	denotes	mimics
T15739	75-79	WDT	denotes	that
T15738	70-74	NN	denotes	MSUD
T15737	67-69	IN	denotes	of
T15736	54-61	JJ	denotes	variant
T15735	62-66	NN	denotes	form
T15734	52-53	DT	denotes	a
T15733	33-35	NN	denotes	E2
T15732	27-32	JJ	denotes	human
T15731	36-45	NN	denotes	transgene
T15730	25-26	DT	denotes	a
T15729	14-24	VBG	denotes	expressing
T15728	46-51	VBP	denotes	model
T15727	9-13	NNS	denotes	mice
T15726	0-8	NN	denotes	Knockout
T16069	2931-2944	RB	denotes	substantially
T16068	2922-2930	VBD	denotes	differed
T16067	2916-2921	NNS	denotes	lines
T16066	2910-2915	DT	denotes	These
T16065	2909-3037	sentence	denotes	These lines differed substantially in transgene copy number as compared by Southern blot analysis of tail DNA (data not shown).
T16064	2908-2909	.	denotes	.
T16063	2903-2908	NNS	denotes	lines
T16062	2897-2902	DT	denotes	these
T16061	2894-2896	IN	denotes	of
T16060	2892-2893	CD	denotes	9
T16059	2889-2891	IN	denotes	of
T16058	2883-2888	NN	denotes	total
T16057	2881-2882	DT	denotes	a
T16056	2878-2880	IN	denotes	on
T16055	2867-2871	RB	denotes	only
T16054	2864-2866	TO	denotes	to
T16053	2872-2877	VB	denotes	focus
T16052	2861-2863	PRP	denotes	us
T16051	2853-2860	VBD	denotes	allowed
T16050	2843-2852	NNS	denotes	resources
T16049	2835-2842	VBN	denotes	Limited
T16048	2834-2909	sentence	denotes	Limited resources allowed us to only focus on a total of 9 of these lines.
T16047	2833-2834	.	denotes	.
T16046	2825-2833	NNS	denotes	founders
T16045	2819-2824	DT	denotes	these
T16044	2814-2818	IN	denotes	from
T16043	2797-2807	JJ	denotes	transgenic
T16042	2787-2796	JJ	denotes	different
T16041	2808-2813	NNS	denotes	lines
T16040	2784-2786	CD	denotes	15
T16039	2781-2783	IN	denotes	of
T16038	2775-2780	NN	denotes	total
T16037	2773-2774	DT	denotes	a
T16036	2761-2772	VBD	denotes	established
T16035	2758-2760	PRP	denotes	We
T16034	2757-2834	sentence	denotes	We established a total of 15 different transgenic lines from these founders.
T16033	2756-2757	.	denotes	.
T16032	2746-2756	VBD	denotes	segregated
T16031	2741-2745	WDT	denotes	that
T16030	2725-2734	NN	denotes	insertion
T16029	2715-2724	NN	denotes	transgene
T16028	2735-2740	NNS	denotes	sites
T16027	2706-2714	JJ	denotes	multiple
T16026	2694-2701	NNS	denotes	animals
T16025	2690-2693	DT	denotes	the
T16024	2687-2689	IN	denotes	of
T16023	2682-2686	JJ	denotes	many
T16022	2702-2705	VBD	denotes	had
T16021	2677-2681	IN	denotes	that
T16020	2659-2667	NNS	denotes	founders
T16019	2653-2658	DT	denotes	these
T16018	2650-2652	IN	denotes	of
T16017	2668-2676	VBD	denotes	revealed
T16016	2641-2649	NN	denotes	breeding
T16015	2630-2640	JJ	denotes	Subsequent
T16014	2629-2757	sentence	denotes	Subsequent breeding of these founders revealed that many of the animals had multiple transgene insertion sites that segregated.
T16013	2628-2629	.	denotes	.
T16012	2611-2619	JJ	denotes	positive
T16011	2601-2610	NN	denotes	transgene
T16010	2620-2628	NNS	denotes	founders
T16009	2599-2600	CD	denotes	8
T16008	2587-2589	PRP	denotes	we
T16007	2585-2587	,	denotes	,
T16006	2575-2585	NNP	denotes	Cincinnati
T16005	2572-2574	IN	denotes	of
T16004	2561-2571	NNP	denotes	University
T16003	2557-2560	DT	denotes	the
T16002	2554-2556	IN	denotes	at
T16001	2543-2553	NNS	denotes	injections
T16000	2539-2542	DT	denotes	the
T15999	2590-2598	VBD	denotes	obtained
T15998	2534-2538	IN	denotes	From
T15997	2533-2629	sentence	denotes	From the injections at the University of Cincinnati, we obtained 8 transgene positive founders.
T15996	2532-2533	.	denotes	.
T15995	2531-2532	-RRB-	denotes	)
T15994	2530-2531	NN	denotes	D
T15993	2528-2529	CC	denotes	&
T15992	2526-2528	,	denotes	,
T15991	2525-2526	NN	denotes	B
T15990	2523-2525	,	denotes	,
T15989	2516-2521	NNS	denotes	Lines
T15988	2522-2523	NN	denotes	A
T15987	2515-2516	-LRB-	denotes	(
T15986	2498-2508	JJ	denotes	transgenic
T15985	2488-2497	JJ	denotes	different
T15984	2509-2514	NNS	denotes	lines
T15983	2486-2487	CD	denotes	3
T15982	2483-2485	IN	denotes	of
T15981	2472-2482	NN	denotes	generation
T15980	2468-2471	DT	denotes	the
T15979	2465-2467	IN	denotes	to
T15978	2461-2464	VBD	denotes	led
T15977	2456-2460	WDT	denotes	that
T15976	2440-2444	VBD	denotes	were
T15975	2422-2430	JJ	denotes	positive
T15974	2412-2421	NN	denotes	transgene
T15973	2431-2439	NNS	denotes	founders
T15972	2410-2411	CD	denotes	2
T15971	2408-2410	,	denotes	,
T15970	2398-2408	NNP	denotes	Pittsburgh
T15969	2395-2397	IN	denotes	of
T15968	2384-2394	NNP	denotes	University
T15967	2380-2383	DT	denotes	the
T15966	2377-2379	IN	denotes	at
T15965	2366-2376	NNS	denotes	injections
T15964	2445-2455	VBN	denotes	identified
T15963	2361-2365	IN	denotes	From
T15962	2360-2533	sentence	denotes	From injections at the University of Pittsburgh, 2 transgene positive founders were identified that led to the generation of 3 different transgenic lines (Lines A, B, & D).
T15961	2359-2360	.	denotes	.
T15960	2346-2347	HYPH	denotes	-
T17015	9305-9311	JJ	denotes	useful
T23928	2174-2175	.	denotes	.
T24488	4570-4572	IN	denotes	of
T24487	4561-4569	NN	denotes	knockout
T24486	4557-4560	IN	denotes	for
T24485	4544-4546	,	denotes	,
T24484	4540-4544	FW	denotes	i.e.
T24483	4546-4556	JJ	denotes	homozygous
T24687	5472-5477	NNS	denotes	cages
T24686	5466-5471	PRP$	denotes	their
T24685	5463-5465	IN	denotes	in
T24684	5458-5462	JJ	denotes	dead
T24683	5452-5457	VBN	denotes	found
T24682	5447-5451	VBD	denotes	were
T24681	5444-5446	CC	denotes	or
T15938	2235-2238	DT	denotes	the
T15937	2232-2234	IN	denotes	by
T15936	2228-2231	VBN	denotes	zed
T15935	2227-2228	NNS	denotes	i
T15934	2227-2262	sentence	denotes	ized by the Bujard laboratory [34].
T15900	2184-2188	NNS	denotes	mice
T15899	2179-2180	HYPH	denotes	-
T15898	2180-2183	NN	denotes	tTA
T15897	2176-2179	NN	denotes	LAP
T15896	2176-2189	sentence	denotes	LAP-tTA mice
T15895	889-890	sentence	denotes
T15894	888-889	.	denotes	.
T15893	885-888	NN	denotes	tTA
T15892	882-884	IN	denotes	by
T15891	870-881	NN	denotes	stimulation
T15890	865-869	IN	denotes	upon
T15889	856-864	NN	denotes	promoter
T15911	2200-2201	NN	denotes	u
T15910	2199-2200	VB	denotes	o
T15909	2199-2205	sentence	denotes	ously
T15908	2198-2199	JJ	denotes	i
T15907	2197-2198	NN	denotes	v
T15906	2194-2197	NN	denotes	pre
T15905	2194-2199	sentence	denotes	previ
T15904	2191-2193	VB	denotes	re
T15903	2189-2190	VBD	denotes	w
T15902	2190-2191	VBN	denotes	e
T15901	2189-2194	sentence	denotes	were
T15917	2208-2209	NN	denotes	d
T15916	2205-2208	NN	denotes	pro
T15915	2205-2212	sentence	denotes	produce
T15914	2203-2204	JJ	denotes	y
T15913	2202-2203	JJ	denotes	l
T15912	2201-2202	NNS	denotes	s
T15959	2347-2349	NN	denotes	E2
T15958	2343-2346	NN	denotes	TRE
T15957	2350-2359	NN	denotes	transgene
T15956	2339-2342	DT	denotes	the
T15955	2330-2338	VBD	denotes	harbored
T15954	2325-2329	WDT	denotes	that
T15953	2320-2324	NNS	denotes	mice
T15952	2309-2319	JJ	denotes	transgenic
T15951	2301-2308	VB	denotes	produce
T15950	2298-2300	TO	denotes	to
T15949	2289-2292	VBD	denotes	was
T15948	2293-2297	VBN	denotes	used
T15947	2274-2288	NN	denotes	microinjection
T15946	2263-2273	JJ	denotes	Pronuclear
T15945	2262-2360	sentence	denotes	Pronuclear microinjection was used to produce transgenic mice that harbored the TRE-E2 transgene.
T15944	2261-2262	.	denotes	.
T15943	2260-2261	-RRB-	denotes	]
T15942	2258-2260	CD	denotes	34
T15941	2257-2258	-LRB-	denotes	[
T15940	2239-2245	NNP	denotes	Bujard
T15939	2246-2256	NN	denotes	laboratory
T15933	2225-2226	VB	denotes	e
T15932	2224-2225	TO	denotes	t
T15931	2223-2224	DT	denotes	c
T15930	2222-2223	VBN	denotes	a
T15929	2221-2222	NN	denotes	r
T15928	2226-2227	VBN	denotes	r
T15927	2220-2221	DT	denotes	a
T15926	2220-2227	sentence	denotes	aracter
T15925	2219-2220	DT	denotes	h
T15924	2218-2219	NN	denotes	c
T15923	2214-2217	CC	denotes	and
T15922	2212-2213	NN	denotes	d
T15921	2212-2220	sentence	denotes	d and ch
T15920	2211-2212	NNS	denotes	e
T15919	2210-2211	JJ	denotes	c
T15918	2209-2210	JJ	denotes	u
T16321	4238-4247	VBG	denotes	surviving
T16320	4222-4228	VBN	denotes	tested
T16319	4229-4237	VBD	denotes	produced
T16318	4210-4215	JJ	denotes	other
T16317	4216-4221	NNS	denotes	lines
T16316	4206-4209	DT	denotes	All
T16315	4205-4310	sentence	denotes	All other lines tested produced surviving rescue animals at a frequency between 1 and 5% of weaned pups.
T16314	4204-4205	.	denotes	.
T16285	4039-4041	TO	denotes	to
T16284	4016-4020	NN	denotes	525A
T16283	4021-4030	NN	denotes	transgene
T16282	4012-4015	DT	denotes	the
T16281	4010-4012	,	denotes	,
T16280	4031-4038	VBZ	denotes	appears
T16279	4006-4010	RB	denotes	Thus
T16278	4005-4123	sentence	denotes	Thus, the 525A transgene appears to be highly effective at rescuing the neonatal lethal phenotype of the E2 knockout.
T16313	4188-4194	JJ	denotes	lethal
T16312	4179-4187	JJ	denotes	neonatal
T16311	4195-4204	NN	denotes	phenotype
T16310	4175-4178	DT	denotes	the
T16309	4168-4174	VB	denotes	rescue
T16308	4165-4167	TO	denotes	to
T16307	4147-4157	RB	denotes	completely
T16306	4142-4146	NN	denotes	520B
T16305	4137-4141	NN	denotes	line
T16304	4135-4137	,	denotes	,
T16303	4127-4135	NN	denotes	contrast
T16302	4158-4164	VBD	denotes	failed
T16301	4124-4126	IN	denotes	In
T16300	4123-4205	sentence	denotes	In contrast, line 520B completely failed to rescue the neonatal lethal phenotype.
T16299	4122-4123	.	denotes	.
T16298	4111-4113	NN	denotes	E2
T16297	4114-4122	NN	denotes	knockout
T16296	4107-4110	DT	denotes	the
T16295	4104-4106	IN	denotes	of
T16294	4087-4093	JJ	denotes	lethal
T16293	4078-4086	JJ	denotes	neonatal
T16292	4094-4103	NN	denotes	phenotype
T16291	4074-4077	DT	denotes	the
T16290	4065-4073	VBG	denotes	rescuing
T16289	4062-4064	IN	denotes	at
T16288	4052-4061	JJ	denotes	effective
T16287	4045-4051	RB	denotes	highly
T16286	4042-4044	VB	denotes	be
T16355	5504-5505	NNS	denotes	e
T16354	5503-5504	NNS	denotes	l
T16353	5502-5503	JJR	denotes	e
T16352	5501-5502	RB	denotes	s
T16351	5496-5500	VBD	denotes	were
T16350	5496-5505	sentence	denotes	were sele
T16349	5495-5496	sentence	denotes
T16348	5491-5495	NN	denotes	525A
T16347	5489-5490	NN	denotes	d
T16346	5487-5489	NN	denotes	an
T16345	5485-5486	NN	denotes	A
T16344	5485-5495	sentence	denotes	A and 525A
T16343	5483-5484	VBZ	denotes	s
T16342	5482-5483	NN	denotes	e
T16341	5481-5482	NN	denotes	n
T16340	5480-5481	VBZ	denotes	i
T16339	5480-5485	sentence	denotes	ines
T16338	5479-5480	NN	denotes	L
T16379	5540-5642	sentence	denotes	Fig. 4B shows the results of blood amino acid analysis presented as a ratio of total BCAA to alanine.
T16378	5539-5540	.	denotes	.
T16377	5528-5539	NN	denotes	cterization
T16376	5527-5528	VBN	denotes	a
T16375	5526-5527	VBD	denotes	r
T16374	5526-5540	sentence	denotes	racterization.
T16373	5525-5526	NNS	denotes	a
T16372	5524-5525	PRP$	denotes	h
T16371	5523-5524	VBN	denotes	c
T16370	5521-5522	JJ	denotes	d
T16369	5520-5521	PRP	denotes	e
T16368	5519-5520	IN	denotes	l
T16367	5517-5519	NN	denotes	ai
T16366	5516-5517	IN	denotes	t
T16365	5516-5526	sentence	denotes	tailed cha
T16364	5515-5516	NNS	denotes	e
T16363	5514-5515	CC	denotes	d
T16362	5511-5513	NN	denotes	or
T16361	5510-5511	IN	denotes	f
T16360	5510-5516	sentence	denotes	for de
T16359	5509-5510	sentence	denotes
T16358	5508-5509	VBN	denotes	d
T16357	5505-5508	VBZ	denotes	cte
T16356	5505-5509	sentence	denotes	cted
T16382	5549-5554	VBZ	denotes	shows
T16381	5546-5548	NN	denotes	4B
T16380	5541-5545	NN	denotes	Fig.
T16501	6777-6781	VBP	denotes	have
T16500	6768-6773	JJ	denotes	human
T16499	6774-6776	NN	denotes	E2
T16498	6757-6767	JJ	denotes	transgenic
T16497	6754-6756	IN	denotes	of
T16496	6743-6753	NN	denotes	expression
T16495	6737-6742	VB	denotes	drive
T16494	6734-6736	TO	denotes	to
T16493	6724-6728	VBD	denotes	were
T16492	6729-6733	VBN	denotes	used
T16491	6719-6723	WDT	denotes	that
T16490	6709-6710	HYPH	denotes	-
T16489	6710-6713	NN	denotes	tTA
T16488	6706-6709	NN	denotes	LAP
T16487	6714-6718	NNS	denotes	mice
T16486	6702-6705	DT	denotes	the
T16485	6787-6799	VBN	denotes	demonstrated
T16484	6694-6701	IN	denotes	Because
T16483	6694-6942	sentence	denotes	Because the LAP-tTA mice that were used to drive expression of transgenic human E2 have been demonstrated to produce liver specific expression [34], BCKDH enzyme activity and production of human E2 protein in liver of Lines A and 525A was examined.
R3835	T15726	T15727	compound	Knockout,mice
R3836	T15727	T15728	nsubj	mice,model
R3837	T15729	T15727	acl	expressing,mice
R3838	T15730	T15731	det	a,transgene
R3839	T15731	T15729	dobj	transgene,expressing
R3840	T15732	T15731	amod	human,transgene
R3841	T15733	T15731	compound	E2,transgene
R3842	T15734	T15735	det	a,form
R3843	T15735	T15728	dobj	form,model
R3844	T15736	T15735	amod	variant,form
R3845	T15737	T15735	prep	of,form
R3846	T15738	T15737	pobj	MSUD,of
R3847	T15739	T15740	dep	that,mimics
R3848	T15740	T15735	relcl	mimics,form
R3849	T15741	T15742	amod	intermediate,MSUD
R3850	T15742	T15740	dobj	MSUD,mimics
R3851	T15744	T15745	aux	To,create
R3852	T15745	T15746	advcl	create,used
R3853	T15747	T15748	det	a,model
R3854	T15748	T15745	dobj	model,create
R3855	T15749	T15748	amod	murine,model
R3856	T15750	T15748	prep	of,model
R3857	T15751	T15752	det	the,form
R3858	T15752	T15750	pobj	form,of
R3859	T15753	T15752	amod	intermediate,form
R3860	T15754	T15752	compound	variant,form
R3861	T15755	T15752	prep	of,form
R3862	T15756	T15755	pobj	MSUD,of
R3863	T15757	T15746	punct	", ",used
R3864	T15758	T15746	nsubj	we,used
R3865	T15759	T15760	det	a,strategy
R3866	T15760	T15746	dobj	strategy,used
R3867	T15761	T15760	amod	transgenic,strategy
R3868	T15762	T15763	aux	to,rescue
R3869	T15763	T15746	advcl	rescue,used
R3870	T15764	T15765	det	the,elevation
R3871	T15765	T15763	dobj	elevation,rescue
R3872	T15766	T15765	amod	severe,elevation
R3873	T15767	T15765	prep	of,elevation
R3874	T15768	T15767	pobj	BCAA,of
R3875	T15769	T15765	cc	and,elevation
R3876	T15770	T15771	amod	neonatal,lethality
R3877	T15771	T15765	conj	lethality,elevation
R3878	T15772	T15773	dep	that,occurs
R3879	T15773	T15771	relcl	occurs,lethality
R3880	T15774	T15773	prep	in,occurs
R3881	T15775	T15776	det	the,model
R3882	T15776	T15774	pobj	model,in
R3883	T15777	T15776	amod	classic,model
R3884	T15778	T15776	compound	MSUD,model
R3885	T15779	T15776	compound	mouse,model
R3886	T15780	T15746	punct	.,used
R3887	T15782	T15783	poss	Our,strategy
R3888	T15783	T15784	nsubjpass	strategy,composed
R3889	T15785	T15784	auxpass	was,composed
R3890	T15786	T15784	prep	of,composed
R3891	T15787	T15788	det	a,system
R3892	T15788	T15786	pobj	system,of
R3893	T15789	T15790	nummod	two,part
R3894	T15790	T15788	nmod	part,system
R3895	T15791	T15788	amod	transgenic,system
R3896	T15792	T15793	aux	to,express
R3897	T15793	T15788	advcl	express,system
R3898	T15794	T15795	amod	human,E2
R3899	T15795	T15793	dobj	E2,express
R3900	T15796	T15793	prep	in,express
R3901	T15797	T15796	pobj	liver,in
R3902	T15798	T15793	prep	on,express
R3903	T15799	T15800	det	the,background
R3904	T15800	T15798	pobj	background,on
R3905	T15801	T15802	compound	E2,knockout
R3906	T15802	T15800	compound	knockout,background
R3907	T15803	T15784	punct	.,composed
R3908	T15805	T15806	det	This,system
R3909	T15806	T15808	nsubj	system,consisted
R3910	T15807	T15806	amod	bi-transgenic,system
R3911	T15809	T15808	prep	of,consisted
R3912	T15810	T15811	det	a,transgene
R3913	T15811	T15809	pobj	transgene,of
R3914	T15812	T15813	compound	LAP,tTA
R3915	T15813	T15811	compound	tTA,transgene
R3916	T15814	T15813	punct	-,tTA
R3917	T15815	T15811	cc	and,transgene
R3918	T15816	T15817	det	a,transgene
R3919	T15817	T15811	conj	transgene,transgene
R3920	T15818	T15819	compound	TRE,E2
R3921	T15819	T15817	compound	E2,transgene
R3922	T15820	T15819	punct	-,E2
R3923	T15821	T15822	punct	(,3A
R3924	T15822	T15808	parataxis	3A,consisted
R3925	T15823	T15822	compound	Fig.,3A
R3926	T15824	T15822	punct	),3A
R3927	T15825	T15808	punct	.,consisted
R3944	T15844	T15842	pobj	transactivator,of
R3945	T15845	T15846	npadvmod	tetracycline,controlled
R3946	T15846	T15844	amod	controlled,transactivator
R3947	T15847	T15846	punct	-,controlled
R3948	T15848	T15844	punct	(,transactivator
R3949	T15849	T15844	appos	tTA,transactivator
R3950	T15850	T15844	punct	),transactivator
R3951	T15851	T15844	punct	", ",transactivator
R3952	T15852	T15853	det	a,factor
R3953	T15853	T15844	appos	factor,transactivator
R3954	T15854	T15853	compound	transcription,factor
R3955	T15855	T15856	dep	that,stimulates
R3956	T15856	T15853	relcl	stimulates,factor
R3957	T15857	T15856	dobj	expression,stimulates
R3958	T15858	T15857	prep	of,expression
R3959	T15859	T15858	pobj	promoters,of
R3960	T15860	T15861	dep	that,harbor
R3961	T15861	T15859	relcl	harbor,promoters
R3962	T15862	T15863	det	a,element
R3963	T15863	T15861	dobj	element,harbor
R3964	T15864	T15863	compound	transactivator,element
R3965	T15865	T15863	compound	response,element
R3966	T15866	T15863	punct	(,element
R3967	T15867	T15863	appos	TRE,element
R3968	T15868	T15832	punct	),directs
R3969	T15869	T15832	punct	.,directs
R3970	T15871	T15872	det	The,transgene
R3971	T15872	T15876	nsubjpass	transgene,designed
R3972	T15873	T15874	compound	TRE,E2
R3973	T15874	T15872	compound	E2,transgene
R3974	T15875	T15874	punct	-,E2
R3975	T15877	T15876	auxpass	was,designed
R3976	T15878	T15879	aux	to,express
R3977	T15879	T15876	advcl	express,designed
R3978	T15880	T15881	det	a,cDNA
R3979	T15881	T15879	dobj	cDNA,express
R3980	T15882	T15881	amod	human,cDNA
R3981	T15883	T15881	compound	E2,cDNA
R3982	T15884	T15879	prep	from,express
R3983	T15885	T15886	det	a,TRE
R3984	T15886	T15884	pobj	TRE,from
R3985	T15887	T15886	acl	containing,TRE
R3986	T15888	T15889	amod	minimal,promoter
R3987	T15889	T15887	dobj	promoter,containing
R3988	T15890	T15879	prep	upon,express
R3989	T15891	T15890	pobj	stimulation,upon
R3990	T15892	T15891	prep	by,stimulation
R3991	T15893	T15892	pobj	tTA,by
R3992	T15894	T15876	punct	.,designed
R4008	T15941	T15942	punct	[,34
R4009	T15942	T15936	parataxis	34,zed
R4010	T15943	T15942	punct	],34
R4011	T15946	T15947	amod	Pronuclear,microinjection
R4012	T15947	T15948	nsubjpass	microinjection,used
R4013	T15949	T15948	auxpass	was,used
R4014	T15950	T15951	aux	to,produce
R4015	T15951	T15948	advcl	produce,used
R4016	T15952	T15953	amod	transgenic,mice
R4017	T15953	T15951	dobj	mice,produce
R4018	T15954	T15955	dep	that,harbored
R4019	T15955	T15953	relcl	harbored,mice
R4020	T15956	T15957	det	the,transgene
R4021	T15957	T15955	dobj	transgene,harbored
R4022	T15958	T15959	compound	TRE,E2
R4023	T15959	T15957	compound	E2,transgene
R4024	T15960	T15959	punct	-,E2
R4025	T15961	T15948	punct	.,used
R4026	T15963	T15964	prep	From,identified
R4027	T15965	T15963	pobj	injections,From
R4028	T15966	T15965	prep	at,injections
R4029	T15967	T15968	det	the,University
R4030	T15968	T15966	pobj	University,at
R4031	T15969	T15968	prep	of,University
R4032	T15970	T15969	pobj	Pittsburgh,of
R4033	T15971	T15964	punct	", ",identified
R4034	T15972	T15973	nummod	2,founders
R4035	T15973	T15964	nsubjpass	founders,identified
R4036	T15974	T15975	npadvmod	transgene,positive
R4037	T15975	T15973	amod	positive,founders
R4038	T15976	T15964	auxpass	were,identified
R4039	T15977	T15978	dep	that,led
R4040	T15978	T15964	ccomp	led,identified
R4041	T15979	T15978	prep	to,led
R4042	T15980	T15981	det	the,generation
R4043	T15981	T15979	pobj	generation,to
R4044	T15982	T15981	prep	of,generation
R4045	T15983	T15984	nummod	3,lines
R4046	T15984	T15982	pobj	lines,of
R4047	T15985	T15984	amod	different,lines
R4048	T15986	T15984	amod	transgenic,lines
R4049	T15987	T15988	punct	(,A
R4050	T15988	T15984	parataxis	A,lines
R4051	T15989	T15988	compound	Lines,A
R4052	T15990	T15988	punct	", ",A
R4053	T15991	T15988	conj	B,A
R4054	T15992	T15991	punct	", ",B
R4055	T15993	T15991	cc	&,B
R4056	T15994	T15991	conj	D,B
R4057	T15995	T15988	punct	),A
R4058	T15996	T15964	punct	.,identified
R4059	T15998	T15999	prep	From,obtained
R4060	T16000	T16001	det	the,injections
R4061	T16001	T15998	pobj	injections,From
R4062	T16002	T16001	prep	at,injections
R4063	T16003	T16004	det	the,University
R4064	T16004	T16002	pobj	University,at
R4065	T16005	T16004	prep	of,University
R4066	T16006	T16005	pobj	Cincinnati,of
R4067	T16007	T15999	punct	", ",obtained
R4068	T16008	T15999	nsubj	we,obtained
R4069	T16009	T16010	nummod	8,founders
R4070	T16010	T15999	dobj	founders,obtained
R4071	T16011	T16012	npadvmod	transgene,positive
R4072	T16012	T16010	amod	positive,founders
R4073	T16013	T15999	punct	.,obtained
R4074	T16015	T16016	amod	Subsequent,breeding
R4075	T16016	T16017	nsubj	breeding,revealed
R4076	T16018	T16016	prep	of,breeding
R4077	T16019	T16020	det	these,founders
R4078	T16020	T16018	pobj	founders,of
R4079	T16021	T16022	mark	that,had
R4080	T16022	T16017	ccomp	had,revealed
R4081	T16023	T16022	nsubj	many,had
R4082	T16024	T16023	prep	of,many
R4083	T16025	T16026	det	the,animals
R4084	T16026	T16024	pobj	animals,of
R4085	T16027	T16028	amod	multiple,sites
R4086	T16028	T16022	dobj	sites,had
R4087	T16029	T16028	compound	transgene,sites
R4088	T16030	T16028	compound	insertion,sites
R4089	T16031	T16032	dep	that,segregated
R4090	T16032	T16028	relcl	segregated,sites
R4091	T16033	T16017	punct	.,revealed
R4092	T16035	T16036	nsubj	We,established
R4093	T16037	T16038	det	a,total
R4094	T16038	T16036	dobj	total,established
R4095	T16039	T16038	prep	of,total
R4096	T16040	T16041	nummod	15,lines
R4097	T16041	T16039	pobj	lines,of
R4098	T16042	T16041	amod	different,lines
R4099	T16043	T16041	amod	transgenic,lines
R4100	T16044	T16036	prep	from,established
R4101	T16045	T16046	det	these,founders
R4102	T16046	T16044	pobj	founders,from
R4103	T16047	T16036	punct	.,established
R4104	T16049	T16050	amod	Limited,resources
R4105	T16050	T16051	nsubj	resources,allowed
R4106	T16052	T16053	nsubj	us,focus
R4107	T16053	T16051	ccomp	focus,allowed
R4108	T16054	T16053	aux	to,focus
R4109	T16055	T16053	advmod	only,focus
R4110	T16056	T16053	prep	on,focus
R4111	T16057	T16058	det	a,total
R4112	T16058	T16056	pobj	total,on
R4113	T16059	T16058	prep	of,total
R4114	T16060	T16059	pobj	9,of
R4115	T16061	T16060	prep	of,9
R4116	T16062	T16063	det	these,lines
R4117	T16063	T16061	pobj	lines,of
R4118	T16064	T16051	punct	.,allowed
R4119	T16066	T16067	det	These,lines
R4120	T16067	T16068	nsubj	lines,differed
R4121	T16069	T16068	advmod	substantially,differed
R4122	T16070	T16068	prep	in,differed
R4123	T16071	T16072	compound	transgene,number
R4124	T16072	T16070	pobj	number,in
R4125	T16073	T16072	compound	copy,number
R4126	T16074	T16075	mark	as,compared
R4127	T16075	T16068	advcl	compared,differed
R4128	T16076	T16075	prep	by,compared
R4129	T16077	T16078	compound	Southern,blot
R4130	T16078	T16079	compound	blot,analysis
R4131	T16079	T16076	pobj	analysis,by
R4132	T16080	T16079	prep	of,analysis
R4133	T16081	T16082	compound	tail,DNA
R4134	T16082	T16080	pobj	DNA,of
R4135	T16083	T16084	punct	(,shown
R4136	T16084	T16068	parataxis	shown,differed
R4137	T16085	T16084	nsubj	data,shown
R4138	T16086	T16084	neg	not,shown
R4139	T16087	T16084	punct	),shown
R4140	T16088	T16068	punct	.,differed
R4141	T16090	T16091	amod	Transgenic,mice
R4142	T16091	T16095	nsubjpass	mice,crossed
R4143	T16092	T16093	compound	TRE,E2
R4144	T16093	T16091	compound	E2,mice
R4145	T16094	T16093	punct	-,E2
R4146	T16096	T16091	prep	from,mice
R4147	T16097	T16098	det	each,line
R4148	T16098	T16096	pobj	line,from
R4149	T16099	T16100	punct	(,offspring
R4150	T16100	T16098	parataxis	offspring,line
R4151	T16101	T16100	preconj	either,offspring
R4152	T16102	T16100	nmod	founders,offspring
R4153	T16103	T16102	cc	or,founders
R4154	T16104	T16102	conj	F1,founders
R4155	T16105	T16100	punct	),offspring
R4156	T16106	T16095	auxpass	were,crossed
R4157	T16107	T16095	prep	to,crossed
R4158	T16108	T16107	pobj	mice,to
R4159	T16109	T16110	dep	that,were
R4160	T16110	T16108	relcl	were,mice
R4161	T16111	T16110	acomp	positive,were
R4162	T16112	T16111	prep	for,positive
R4163	T16113	T16114	det	the,transgene
R4164	T16114	T16112	pobj	transgene,for
R4165	T16115	T16116	compound	LAP,tTA
R4166	T16116	T16114	compound	tTA,transgene
R4167	T16117	T16116	punct	-,tTA
R4168	T16118	T16110	cc	and,were
R4169	T16119	T16110	conj	were,were
R4170	T16120	T16119	acomp	heterozygous,were
R4171	T16121	T16120	prep	for,heterozygous
R4172	T16122	T16123	det	the,knockout
R4173	T16123	T16121	pobj	knockout,for
R4174	T16124	T16123	compound	E2,knockout
R4175	T16125	T16095	punct	.,crossed
R4176	T16127	T16128	advmod	Ultimately,used
R4177	T16129	T16128	punct	", ",used
R4178	T16130	T16131	amod	breeding,pairs
R4179	T16131	T16128	nsubjpass	pairs,used
R4180	T16132	T16128	auxpass	were,used
R4181	T16133	T16134	prep	in,were
R4182	T16134	T16128	ccomp	were,used
R4183	T16135	T16133	pobj	which,in
R4184	T16136	T16137	det	the,mice
R4185	T16137	T16134	nsubj	mice,were
R4186	T16138	T16134	acomp	heterozygous,were
R4187	T16139	T16138	prep	for,heterozygous
R4188	T16140	T16141	det	the,transgene
R4189	T16141	T16139	pobj	transgene,for
R4190	T16142	T16143	compound	LAP,tTA
R4191	T16143	T16141	compound	tTA,transgene
R4192	T16144	T16143	punct	-,tTA
R4193	T16145	T16141	punct	", ",transgene
R4194	T16146	T16147	det	the,transgene
R4195	T16147	T16141	conj	transgene,transgene
R4196	T16148	T16149	compound	TRE,E2
R4197	T16149	T16147	compound	E2,transgene
R4198	T16150	T16149	punct	-,E2
R4199	T16151	T16147	punct	", ",transgene
R4200	T16152	T16147	cc	and,transgene
R4201	T16153	T16154	det	the,knockout
R4202	T16154	T16147	conj	knockout,transgene
R4203	T16155	T16154	compound	E2,knockout
R4204	T16156	T16128	punct	.,used
R4205	T16158	T16159	aux	To,screen
R4206	T16159	T16161	advcl	screen,genotyped
R4207	T16160	T16159	advmod	efficiently,screen
R4208	T16162	T16159	prep	for,screen
R4209	T16163	T16164	det	the,ability
R4210	T16164	T16162	pobj	ability,for
R4211	T16165	T16164	prep	of,ability
R4212	T16166	T16167	det	the,transgenes
R4213	T16167	T16165	pobj	transgenes,of
R4214	T16168	T16169	aux	to,rescue
R4215	T16169	T16164	acl	rescue,ability
R4216	T16170	T16171	det	the,knockout
R4217	T16171	T16169	dobj	knockout,rescue
R4218	T16172	T16169	prep	from,rescue
R4219	T16173	T16174	amod	neonatal,lethality
R4220	T16174	T16172	pobj	lethality,from
R4221	T16175	T16161	punct	", ",genotyped
R4222	T16176	T16161	nsubj	we,genotyped
R4223	T16177	T16161	dobj	litters,genotyped
R4224	T16178	T16161	prep	at,genotyped
R4225	T16179	T16178	pobj	weaning,at
R4226	T16180	T16161	punct	.,genotyped
R4227	T16182	T16183	det	This,strategy
R4228	T16183	T16185	nsubjpass	strategy,expected
R4229	T16184	T16183	amod	breeding,strategy
R4230	T16186	T16185	auxpass	is,expected
R4231	T16187	T16188	aux	to,result
R4232	T16188	T16185	xcomp	result,expected
R4233	T16189	T16188	prep	in,result
R4234	T16190	T16191	det	a,maximum
R4235	T16191	T16189	pobj	maximum,in
R4236	T16192	T16191	amod	theoretical,maximum
R4237	T16193	T16191	prep	of,maximum
R4238	T16194	T16193	pobj	animals,of
R4239	T16195	T16194	prep	with,animals
R4240	T16196	T16197	det	the,genotype
R4241	T16197	T16195	pobj	genotype,with
R4242	T16198	T16197	compound	rescue,genotype
R4243	T16199	T16200	punct	(,knockout
R4244	T16200	T16197	parataxis	knockout,genotype
R4245	T16201	T16200	advmod	i.e.,knockout
R4246	T16202	T16200	punct	", ",knockout
R4247	T16203	T16200	amod	homozygous,knockout
R4248	T16204	T16200	compound	E2,knockout
R4249	T16205	T16200	cc	and,knockout
R4250	T16206	T16200	conj	positive,knockout
R4251	T16207	T16206	prep	for,positive
R4252	T16208	T16209	det	both,transgenes
R4253	T16209	T16207	pobj	transgenes,for
R4254	T16210	T16200	punct	),knockout
R4255	T16211	T16191	prep	of,maximum
R4256	T16212	T16213	nummod	14,%
R4257	T16213	T16211	pobj	%,of
R4258	T16214	T16185	punct	.,expected
R4259	T16216	T16217	compound	Fig.,4A
R4260	T16217	T16218	nsubj	4A,shows
R4261	T16219	T16220	det	the,percentage
R4262	T16220	T16218	dobj	percentage,shows
R4263	T16221	T16220	prep	of,percentage
R4264	T16222	T16221	pobj	mice,of
R4265	T16223	T16222	amod	alive,mice
R4266	T16224	T16223	prep	at,alive
R4267	T16225	T16224	pobj	weaning,at
R4268	T16226	T16220	prep	with,percentage
R4269	T16227	T16228	det	the,genotype
R4270	T16228	T16226	pobj	genotype,with
R4271	T16229	T16228	compound	rescue,genotype
R4272	T16230	T16228	prep	from,genotype
R4273	T16231	T16232	det	each,line
R4274	T16232	T16230	pobj	line,from
R4275	T16279	T16280	advmod	Thus,appears
R4276	T16233	T16232	amod	transgenic,line
R4277	T16281	T16280	punct	", ",appears
R4278	T16234	T16232	acl	tested,line
R4279	T16282	T16283	det	the,transgene
R4280	T16283	T16280	nsubj	transgene,appears
R4281	T16235	T16218	punct	.,shows
R4282	T16284	T16283	compound	525A,transgene
R4283	T16285	T16286	aux	to,be
R4284	T16237	T16238	amod	Considerable,variability
R4285	T16286	T16280	xcomp	be,appears
R4286	T16287	T16288	advmod	highly,effective
R4287	T16288	T16286	acomp	effective,be
R4288	T16289	T16288	prep	at,effective
R4289	T16238	T16239	nsubjpass	variability,observed
R4290	T16240	T16239	auxpass	was,observed
R4293	T16241	T16239	prep	between,observed
R4297	T16242	T16241	pobj	lines,between
R4302	T16243	T16239	punct	.,observed
R4305	T16245	T16246	det	The,line
R4309	T16246	T16247	nsubj	line,was
R4311	T16248	T16246	prep	with,line
R4316	T16249	T16250	det	the,percentage
R4318	T16250	T16248	pobj	percentage,with
R4322	T16251	T16250	amod	highest,percentage
R4326	T16252	T16250	prep	of,percentage
R4328	T16253	T16254	compound	rescue,mice
R4332	T16254	T16252	pobj	mice,of
R4336	T16255	T16250	prep	at,percentage
R4340	T16256	T16255	pobj	weaning,at
R4344	T16257	T16258	nmod	line,A
R4345	T16333	T16334	amod	weaned,pups
R4346	T16334	T16332	pobj	pups,of
R4347	T16335	T16319	punct	.,produced
R4348	T16258	T16247	attr	A,was
R4349	T16259	T16258	nummod	525,A
R4350	T16260	T16247	punct	.,was
R4351	T16262	T16263	advmod	Approximately,10
R4352	T16263	T16264	nummod	10,%
R4354	T16264	T16265	nsubj	%,had
R4355	T16266	T16264	prep	of,%
R4356	T16267	T16268	amod	weaned,pups
R4357	T16268	T16266	pobj	pups,of
R4358	T16269	T16268	punct	", ",pups
R4359	T16270	T16268	amod	surviving,pups
R4362	T16271	T16264	prep	from,%
R4363	T16272	T16273	det	this,line
R4364	T16273	T16271	pobj	line,from
R4365	T16274	T16275	det	the,genotype
R4366	T16275	T16265	dobj	genotype,had
R4367	T16276	T16275	compound	rescue,genotype
R4368	T16277	T16265	punct	.,had
R4406	T16399	T16382	punct	.,shows
R4410	T16401	T16402	det	The,values
R4412	T16402	T16406	nsubj	values,were
R4413	T16403	T16404	compound	BCAA,alanine
R4414	T16404	T16402	compound	alanine,values
R4416	T16405	T16404	punct	/,alanine
R4420	T16407	T16402	prep	for,values
R4424	T16408	T16407	pobj	mice,for
R4428	T16409	T16408	prep	from,mice
R4432	T16410	T16411	det	both,lines
R4436	T16411	T16409	pobj	lines,from
R4439	T16521	T16520	compound	E2,protein
R4440	T16412	T16413	advmod	significantly,elevated
R4441	T16522	T16514	prep	in,activity
R4442	T16523	T16522	pobj	liver,in
R4443	T16524	T16523	prep	of,liver
R4444	T16413	T16406	acomp	elevated,were
R4445	T16525	T16526	compound	Lines,A
R4446	T16526	T16524	pobj	A,of
R4447	T16527	T16526	cc	and,A
R4448	T16414	T16406	prep	compared,were
R4449	T16528	T16526	conj	525A,A
R4450	T16529	T16503	auxpass	was,examined
R4451	T16415	T16414	prep	to,compared
R4452	T16530	T16503	punct	.,examined
R4453	T16416	T16415	pobj	controls,to
R4454	T16417	T16416	prep	for,controls
R4455	T16532	T16533	nmod	BCKDH,activity
R4456	T16533	T16535	nsubj	activity,was
R4457	T16534	T16533	amod	enzymatic,activity
R4458	T16418	T16417	pobj	all,for
R4459	T16536	T16533	prep	in,activity
R4460	T16537	T16536	pobj	liver,in
R4461	T16538	T16537	prep	from,liver
R4462	T16539	T16540	compound	Lines,A
R4463	T16419	T16418	prep	of,all
R4464	T16540	T16538	pobj	A,from
R4465	T16541	T16540	cc	and,A
R4466	T16420	T16421	det	the,points
R4467	T16542	T16540	conj	525A,A
R4468	T16543	T16544	punct	~,6
R4469	T16544	T16545	nummod	6,%
R4470	T16421	T16419	pobj	points,of
R4471	T16545	T16535	attr	%,was
R4472	T16546	T16544	cc	and,6
R4473	T16422	T16421	compound	time,points
R4474	T16547	T16544	conj	5,6
R4475	T16548	T16535	punct	", ",was
R4476	T16549	T16535	advmod	respectively,was
R4477	T16423	T16421	acl	analyzed,points
R4478	T16550	T16535	punct	", ",was
R4479	T16551	T16535	prep	of,was
R4480	T16552	T16553	det	the,activity
R4481	T16424	T16406	punct	.,were
R4482	T16553	T16551	pobj	activity,of
R4483	T16554	T16553	amod	enzymatic,activity
R4484	T16555	T16553	amod	present,activity
R4485	T16427	T16428	mark	that,are
R4486	T16556	T16555	prep	in,present
R4487	T16557	T16558	compound	control,liver
R4488	T16558	T16556	pobj	liver,in
R4489	T16559	T16560	punct	(,4C
R4490	T16560	T16535	parataxis	4C,was
R4491	T16428	T16426	ccomp	are,Note
R4492	T16561	T16560	compound	Fig.,4C
R4493	T16562	T16560	punct	),4C
R4494	T16563	T16535	punct	.,was
R4495	T16429	T16430	det	the,values
R4496	T16565	T16566	mark	As,shown
R4497	T16430	T16428	nsubj	values,are
R4498	T16566	T16567	advcl	shown,was
R4499	T16568	T16566	prep	in,shown
R4500	T16431	T16432	compound	BCAA,alanine
R4501	T16569	T16570	compound	Fig.,3B
R4502	T16570	T16568	pobj	3B,in
R4503	T16571	T16567	punct	", ",was
R4504	T16432	T16430	compound	alanine,values
R4505	T16572	T16573	det	the,amount
R4506	T16573	T16567	nsubj	amount,was
R4507	T16574	T16573	prep	of,amount
R4508	T16575	T16576	amod	human,E2
R4509	T16576	T16574	pobj	E2,of
R4510	T16433	T16432	punct	/,alanine
R4511	T16577	T16578	punct	(,Kd
R4512	T16578	T16576	parataxis	Kd,E2
R4513	T16579	T16580	amod	predicted,MW
R4514	T16580	T16578	nsubj	MW,Kd
R4515	T16434	T16430	prep	for,values
R4516	T16581	T16582	punct	~,54
R4517	T16582	T16578	nummod	54,Kd
R4518	T16583	T16578	punct	),Kd
R4519	T16435	T16436	det	the,mice
R4520	T16584	T16573	prep	in,amount
R4521	T16585	T16586	det	these,mice
R4522	T16586	T16584	pobj	mice,in
R4523	T16436	T16434	pobj	mice,for
R4524	T16587	T16586	amod	transgenic,mice
R4525	T16588	T16589	advmod	quite,variable
R4526	T16589	T16567	acomp	variable,was
R4527	T16437	T16436	amod	transgenic,mice
R4528	T16590	T16589	prep	between,variable
R4529	T16438	T16428	acomp	intermediate,are
R4530	T16439	T16428	prep	between,are
R4531	T16591	T16590	pobj	mice,between
R4532	T16592	T16567	punct	.,was
R4534	T16594	T16595	prep	In,was
R4535	T16596	T16594	pobj	some,In
R4536	T16597	T16596	prep	of,some
R4538	T16598	T16599	det	these,mice
R4539	T16599	T16597	pobj	mice,of
R4541	T16600	T16599	amod	transgenic,mice
R4542	T16601	T16595	punct	", ",was
R4543	T16602	T16603	det	the,level
R4545	T16603	T16595	nsubj	level,was
R4546	T16604	T16603	prep	of,level
R4547	T16605	T16606	amod	human,E2
R4549	T16606	T16604	pobj	E2,of
R4550	T16607	T16608	advmod	approximately,equal
R4554	T16608	T16595	acomp	equal,was
R4555	T16609	T16608	prep	to,equal
R4557	T16610	T16611	det	the,amount
R4558	T16611	T16609	pobj	amount,to
R4559	T16612	T16611	prep	of,amount
R4560	T16613	T16614	compound	mouse,E2
R4561	T16614	T16612	pobj	E2,of
R4562	T16615	T16616	punct	(,Kd
R4564	T16616	T16614	parataxis	Kd,E2
R4566	T16617	T16618	punct	~,47
R4567	T16618	T16616	nummod	47,Kd
R4569	T16619	T16616	punct	),Kd
R4570	T16620	T16611	acl	produced,amount
R4571	T16621	T16620	prep	in,produced
R4572	T16622	T16621	pobj	liver,in
R4573	T16623	T16622	prep	of,liver
R4575	T16624	T16625	amod	nontransgenic,mice
R4576	T16625	T16623	pobj	mice,of
R4578	T16626	T16625	compound	control,mice
R4579	T16627	T16595	punct	.,was
R4581	T16629	T16630	det	The,observations
R4582	T16630	T16631	nsubj	observations,suggest
R4584	T16632	T16633	mark	that,result
R4585	T16633	T16630	acl	result,observations
R4587	T16634	T16635	det	these,amounts
R4588	T16635	T16633	nsubj	amounts,result
R4589	T16636	T16637	advmod	near,normal
R4591	T16637	T16635	amod	normal,amounts
R4592	T16638	T16635	prep	of,amounts
R4594	T16639	T16640	compound	E2,protein
R4595	T16640	T16638	pobj	protein,of
R4596	T16641	T16633	prep	in,result
R4598	T16642	T16643	advmod	only,6
R4599	T16643	T16647	nummod	6,%
R4600	T16644	T16643	punct	~,6
R4602	T16645	T16643	quantmod	5,6
R4603	T16646	T16643	punct	–,6
R4604	T16647	T16641	pobj	%,in
R4606	T16648	T16647	prep	of,%
R4607	T16649	T16650	amod	normal,activity
R4608	T16650	T16648	pobj	activity,of
R4610	T16651	T16650	compound	BCKDH,activity
R4611	T16652	T16650	compound	enzyme,activity
R4612	T16653	T16654	mark	that,functioning
R4613	T16654	T16631	ccomp	functioning,suggest
R4614	T16655	T16656	nmod	transgene,E2
R4615	T16656	T16654	nsubj	E2,functioning
R4617	T16657	T16656	amod	derived,E2
R4618	T16658	T16654	aux	is,functioning
R4619	T16659	T16654	prep	at,functioning
R4621	T16660	T16661	det	a,level
R4622	T16661	T16659	pobj	level,at
R4624	T16662	T16661	amod	suboptimal,level
R4625	T16663	T16631	punct	.,suggest
R4627	T16665	T16666	nsubj	It,is
R4628	T16667	T16666	acomp	probable,is
R4630	T16668	T16669	mark	that,interfered
R4631	T16669	T16666	ccomp	interfered,is
R4632	T16670	T16671	det	the,tag
R4634	T16671	T16669	nsubj	tag,interfered
R4635	T16672	T16673	compound	c,myc
R4637	T16673	T16671	compound	myc,tag
R4638	T16674	T16673	punct	-,myc
R4639	T16675	T16671	prep	at,tag
R4641	T16676	T16677	det	the,terminus
R4642	T16677	T16675	pobj	terminus,at
R4643	T16678	T16677	compound	carboxy,terminus
R4645	T16679	T16677	prep	of,terminus
R4646	T16680	T16681	det	the,E2
R4647	T16681	T16679	pobj	E2,of
R4649	T16682	T16683	npadvmod	transgene,derived
R4650	T16683	T16681	amod	derived,E2
R4651	T16684	T16681	amod	human,E2
R4653	T16685	T16669	prep	with,interfered
R4654	T16686	T16687	amod	enzymatic,activity
R4655	T16687	T16685	pobj	activity,with
R4657	T16688	T16666	punct	.,is
R4659	T16690	T16691	det	This,interpretation
R4660	T16691	T16692	nsubj	interpretation,is
R4662	T16693	T16692	acomp	consistent,is
R4663	T16694	T16693	prep	with,consistent
R4664	T16695	T16696	amod	previous,studies
R4665	T16696	T16694	pobj	studies,with
R4666	T16697	T16698	dep	which,revealed
R4668	T16698	T16696	relcl	revealed,studies
R4669	T16699	T16698	aux	have,revealed
R4670	T16700	T16701	mark	that,is
R4673	T16701	T16698	ccomp	is,revealed
R4674	T16702	T16703	det	the,terminus
R4675	T16714	T16701	acomp	essential,is
R4676	T16703	T16701	nsubj	terminus,is
R4677	T16704	T16703	compound	carboxy,terminus
R4678	T16705	T16703	prep	of,terminus
R4679	T16706	T16707	det	an,subunit
R4680	T16707	T16705	pobj	subunit,of
R4681	T16708	T16707	amod	analogous,subunit
R4682	T16715	T16714	prep	for,essential
R4683	T16709	T16707	prep	of,subunit
R4684	T16710	T16711	det	the,complex
R4685	T16711	T16709	pobj	complex,of
R4686	T16716	T16717	compound	subunit,interactions
R4687	T16712	T16711	compound	pyruvate,complex
R4688	T16713	T16711	compound	dehydrogenase,complex
R4689	T16717	T16715	pobj	interactions,for
R4690	T16718	T16719	punct	[,35
R4691	T16719	T16701	parataxis	35,is
R4692	T16820	T16819	pobj	E2,of
R4693	T16821	T16808	acomp	negligible,is
R4694	T16720	T16719	punct	],35
R4695	T16822	T16808	prep	in,is
R4696	T16823	T16824	det	those,tissues
R4697	T16824	T16822	pobj	tissues,in
R4698	T16721	T16701	cc	and,is
R4699	T16825	T16808	punct	.,is
R4700	T16722	T16723	nsubj	insertion,interfered
R4701	T16827	T16828	amod	Long,term
R4702	T16828	T16830	compound	term,survival
R4703	T16829	T16828	punct	-,term
R4704	T16723	T16701	conj	interfered,is
R4705	T16830	T16831	nsubjpass	survival,plotted
R4706	T16724	T16722	prep	of,insertion
R4707	T16832	T16830	prep	of,survival
R4708	T16833	T16834	det	the,mice
R4709	T16834	T16832	pobj	mice,of
R4710	T16725	T16726	det	a,tag
R4711	T16835	T16834	prep	from,mice
R4712	T16836	T16837	det	these,lines
R4713	T16837	T16835	pobj	lines,from
R4714	T16838	T16837	nummod	two,lines
R4715	T16839	T16837	amod	transgenic,lines
R4716	T16840	T16841	dep	that,survived
R4717	T16726	T16724	pobj	tag,of
R4718	T16841	T16837	relcl	survived,lines
R4719	T16842	T16841	prep	beyond,survived
R4720	T16843	T16842	pobj	weaning,beyond
R4721	T16727	T16726	compound	Hisx6,tag
R4722	T16844	T16831	auxpass	is,plotted
R4723	T16845	T16831	prep	in,plotted
R4724	T16846	T16847	compound	Fig.,4D
R4725	T16728	T16722	prep	on,insertion
R4726	T16847	T16845	pobj	4D,in
R4727	T16848	T16831	punct	.,plotted
R4728	T16729	T16730	det	the,terminus
R4729	T16850	T16851	mark	Although,collected
R4730	T16730	T16728	pobj	terminus,on
R4731	T16851	T16859	advcl	collected,is
R4732	T16852	T16853	compound	survival,data
R4733	T16853	T16851	nsubjpass	data,collected
R4734	T16854	T16853	prep	for,data
R4735	T16731	T16730	compound	carboxy,terminus
R4736	T16855	T16856	compound	control,animals
R4737	T16856	T16854	pobj	animals,for
R4738	T16857	T16851	auxpass	was,collected
R4739	T16732	T16730	prep	of,terminus
R4740	T16858	T16851	neg	not,collected
R4741	T16860	T16859	punct	", ",is
R4742	T16733	T16734	det	an,subunit
R4743	T16861	T16859	nsubj	it,is
R4744	T16862	T16863	advmod	readily,apparent
R4745	T16734	T16732	pobj	subunit,of
R4746	T16863	T16859	acomp	apparent,is
R4747	T16864	T16865	mark	that,compromised
R4748	T16865	T16859	ccomp	compromised,is
R4749	T16735	T16734	amod	analogous,subunit
R4750	T16866	T16865	nsubjpass	survival,compromised
R4751	T16867	T16866	prep	of,survival
R4752	T16868	T16869	det	the,mice
R4753	T16736	T16737	nmod	E.,coli
R4754	T16869	T16867	pobj	mice,of
R4755	T16870	T16869	compound	rescue,mice
R4756	T16871	T16865	auxpass	was,compromised
R4757	T16737	T16734	nmod	coli,subunit
R4758	T16872	T16859	punct	.,is
R4759	T16874	T16875	prep	By,were
R4760	T16738	T16723	prep	with,interfered
R4761	T16876	T16877	nummod	16,weeks
R4762	T16739	T16740	amod	normal,assembly
R4763	T16877	T16874	pobj	weeks,By
R4764	T16878	T16875	punct	", ",were
R4765	T16879	T16880	det	all,mice
R4766	T16740	T16738	pobj	assembly,with
R4767	T16880	T16875	nsubj	mice,were
R4768	T16881	T16880	prep	of,mice
R4769	T16741	T16740	compound	subunit,assembly
R4770	T16882	T16883	compound	Line,A
R4771	T16883	T16881	pobj	A,of
R4772	T16884	T16875	acomp	moribund,were
R4773	T16742	T16743	punct	[,36
R4774	T16885	T16884	cc	and,moribund
R4775	T16886	T16887	advmod	humanely,sacrificed
R4776	T16887	T16884	conj	sacrificed,moribund
R4777	T16743	T16723	parataxis	36,interfered
R4778	T16888	T16875	punct	", ",were
R4779	T16889	T16875	cc	or,were
R4780	T16744	T16743	punct	],36
R4781	T16890	T16891	auxpass	were,found
R4782	T16891	T16875	conj	found,were
R4783	T16892	T16891	advcl	dead,found
R4784	T16745	T16692	punct	.,is
R4785	T16893	T16891	prep	in,found
R4786	T16894	T16895	poss	their,cage
R4787	T16747	T16748	nsubj	It,is
R4788	T16895	T16893	pobj	cage,in
R4789	T16896	T16875	punct	.,were
R4790	T16898	T16899	nsubj	Survival,appeared
R4791	T16749	T16748	advmod	also,is
R4792	T16900	T16898	prep	of,Survival
R4793	T16901	T16902	compound	Line,525A
R4794	T16902	T16900	pobj	525A,of
R4795	T16750	T16748	acomp	possible,is
R4796	T16903	T16904	aux	to,be
R4797	T16904	T16899	xcomp	be,appeared
R4798	T16905	T16906	advmod	somewhat,better
R4799	T16751	T16752	mark	that,was
R4800	T16906	T16904	acomp	better,be
R4801	T16907	T16899	punct	.,appeared
R4802	T16752	T16748	ccomp	was,is
R4803	T16909	T16910	prep	At,were
R4804	T16753	T16754	amod	human,E2
R4805	T16911	T16912	nummod	20,weeks
R4806	T16912	T16909	pobj	weeks,At
R4807	T16913	T16912	prep	of,weeks
R4808	T16754	T16752	nsubj	E2,was
R4809	T16914	T16913	pobj	age,of
R4810	T16915	T16910	punct	", ",were
R4811	T16916	T16917	punct	~,12
R4812	T16755	T16752	neg	not,was
R4813	T16917	T16918	nummod	12,%
R4814	T16918	T16910	nsubj	%,were
R4815	T16919	T16918	prep	of,%
R4816	T16756	T16757	advmod	fully,functional
R4817	T16920	T16919	pobj	mice,of
R4818	T16921	T16922	punct	(,2
R4819	T16922	T16920	parataxis	2,mice
R4820	T16923	T16922	prep	of,2
R4821	T16924	T16923	pobj	16,of
R4822	T16757	T16752	acomp	functional,was
R4823	T16925	T16922	punct	),2
R4824	T16758	T16759	advmod	when,complexed
R4825	T16759	T16752	advcl	complexed,was
R4826	T16760	T16759	prep	with,complexed
R4827	T16926	T16910	advmod	still,were
R4828	T16927	T16910	acomp	alive,were
R4829	T16761	T16762	compound	mouse,E1
R4830	T16928	T16910	punct	.,were
R4831	T16762	T16760	pobj	E1,with
R4832	T16930	T16931	det	These,survivors
R4833	T16931	T16934	nsubj	survivors,died
R4834	T16932	T16931	nummod	two,survivors
R4835	T16763	T16762	cc	and,E1
R4836	T16933	T16931	amod	rare,survivors
R4837	T16764	T16765	compound	E3,subunits
R4838	T16935	T16934	prep	at,died
R4839	T16936	T16937	nummod	40,weeks
R4840	T16937	T16935	pobj	weeks,at
R4841	T16765	T16762	conj	subunits,E1
R4842	T16938	T16936	cc	and,40
R4843	T16766	T16748	punct	.,is
R4844	T16939	T16936	conj	60,40
R4845	T16940	T16937	prep	of,weeks
R4846	T16941	T16940	pobj	age,of
R4847	T16942	T16934	punct	.,died
R4848	T16768	T16769	amod	Human,E2
R4849	T16944	T16945	prep	In,had
R4850	T16946	T16944	pobj	summary,In
R4851	T16769	T16770	nsubj	E2,shares
R4852	T16947	T16945	punct	", ",had
R4853	T16948	T16949	det	these,mice
R4854	T16771	T16772	punct	~,88
R4855	T16949	T16945	nsubj	mice,had
R4856	T16950	T16949	amod	surviving,mice
R4857	T16951	T16952	compound	BCAA,alanine
R4858	T16952	T16954	compound	alanine,ratios
R4859	T16772	T16773	nummod	88,%
R4860	T16953	T16952	punct	/,alanine
R4861	T16954	T16945	dobj	ratios,had
R4862	T16955	T16956	dep	that,were
R4863	T16773	T16774	compound	%,identity
R4864	T16956	T16954	relcl	were,ratios
R4865	T16957	T16956	acomp	intermediate,were
R4866	T16958	T16957	prep	between,intermediate
R4867	T16774	T16770	dobj	identity,shares
R4868	T16959	T16958	pobj	controls,between
R4869	T16960	T16959	cc	and,controls
R4870	T16961	T16959	conj	knockouts,controls
R4871	T16962	T16945	cc	and,had
R4872	T16963	T16964	nsubj	they,expressed
R4873	T16775	T16774	prep	to,identity
R4874	T16964	T16945	conj	expressed,had
R4875	T16965	T16966	punct	~,6
R4876	T16776	T16777	compound	mouse,E2
R4877	T16777	T16775	pobj	E2,to
R4878	T16966	T16969	nummod	6,%
R4879	T16778	T16770	prep	at,shares
R4880	T16967	T16966	quantmod	5,6
R4881	T16968	T16966	punct	–,6
R4882	T16969	T16964	dobj	%,expressed
R4883	T16779	T16780	det	the,level
R4884	T16970	T16969	prep	of,%
R4885	T16971	T16972	amod	normal,activity
R4886	T16780	T16778	pobj	level,at
R4887	T16972	T16970	pobj	activity,of
R4888	T16973	T16972	compound	BCKDH,activity
R4889	T16974	T16972	compound	enzyme,activity
R4890	T16781	T16782	compound	amino,acid
R4891	T16975	T16972	prep	in,activity
R4892	T16976	T16977	det	the,liver
R4893	T16782	T16780	compound	acid,level
R4894	T16977	T16975	pobj	liver,in
R4895	T16978	T16945	punct	.,had
R4896	T16783	T16770	punct	.,shares
R4897	T16980	T16981	det	These,observations
R4898	T16785	T16786	nsubj	We,used
R4899	T16981	T16983	nsubj	observations,are
R4900	T16982	T16981	amod	phenotypic,observations
R4901	T16984	T16985	advmod	remarkably,similar
R4902	T16985	T16983	acomp	similar,are
R4903	T16787	T16786	advmod	also,used
R4904	T16986	T16985	prep	to,similar
R4905	T16987	T16988	det	the,phenotype
R4906	T16988	T16986	pobj	phenotype,to
R4907	T16788	T16789	compound	western,blot
R4908	T16789	T16790	compound	blot,analysis
R4909	T16989	T16988	amod	clinical,phenotype
R4910	T16790	T16786	dobj	analysis,used
R4911	T16990	T16988	acl	observed,phenotype
R4912	T16991	T16990	prep	in,observed
R4913	T16992	T16993	compound	MSUD,patients
R4914	T16791	T16792	aux	to,analyze
R4915	T16993	T16991	pobj	patients,in
R4916	T16994	T16993	prep	with,patients
R4917	T16995	T16996	det	the,form
R4918	T16792	T16786	advcl	analyze,used
R4919	T16996	T16994	pobj	form,with
R4920	T16997	T16996	amod	intermediate,form
R4921	T16998	T16996	prep	of,form
R4922	T16999	T17000	det	the,disease
R4923	T17000	T16998	pobj	disease,of
R4924	T17001	T17002	punct	[,1
R4925	T16793	T16792	dobj	expression,analyze
R4926	T17002	T16983	parataxis	1,are
R4927	T17003	T17002	punct	],1
R4928	T17004	T16983	punct	.,are
R4929	T16794	T16793	prep	of,expression
R4930	T17006	T17007	advmod	Thus,represent
R4931	T16795	T16796	det	the,transgene
R4932	T17008	T17007	punct	", ",represent
R4933	T17009	T17010	det	these,mice
R4934	T17010	T17007	nsubj	mice,represent
R4935	T16796	T16794	pobj	transgene,of
R4936	T17011	T17010	compound	rescue,mice
R4937	T17012	T17013	det	a,model
R4938	T17013	T17007	dobj	model,represent
R4939	T16797	T16796	compound	E2,transgene
R4940	T17014	T17015	advmod	very,useful
R4941	T17015	T17013	amod	useful,model
R4942	T16798	T16792	prep	in,analyze
R4943	T17016	T17013	prep	of,model
R4944	T17017	T17018	det	the,phenotype
R4945	T17018	T17016	pobj	phenotype,of
R4946	T16799	T16798	pobj	brain,in
R4947	T17019	T17018	amod	intermediate,phenotype
R4948	T17020	T17018	compound	MSUD,phenotype
R4949	T17021	T17007	punct	.,represent
R4950	T16800	T16799	punct	", ",brain
R4951	T16801	T16799	conj	kidney,brain
R4952	T16802	T16801	cc	and,kidney
R4953	T16803	T16801	conj	muscle,kidney
R4954	T16804	T16786	punct	.,used
R4955	T16806	T16807	mark	As,shown
R4956	T16807	T16808	advcl	shown,is
R4957	T16809	T16807	prep	in,shown
R4958	T16810	T16811	compound	Figure,3C
R4959	T16811	T16809	pobj	3C,in
R4960	T16812	T16811	punct	", ",3C
R4961	T16813	T16811	conj	D,3C
R4962	T16814	T16813	cc	and,D
R4963	T16815	T16813	conj	3E,D
R4964	T16816	T16807	advmod	respectively,shown
R4965	T16817	T16808	punct	", ",is
R4966	T16818	T16808	nsubj	expression,is
R4967	T16819	T16818	prep	of,expression
R6739	T23655	T23656	amod	Transgenic,production
R6740	T23657	T23656	compound	mouse,production
R6741	T23658	T23656	cc	and,production
R6742	T23659	T23656	conj	characterization,production
R6743	T23660	T23656	punct	.,production
R6744	T23662	T23663	meta	A,strategy
R6745	T23664	T23663	punct	", ",strategy
R6746	T23665	T23663	amod	Transgenic,strategy
R6747	T23666	T23663	acl	used,strategy
R6748	T23667	T23668	aux	to,produce
R6749	T23668	T23666	advcl	produce,used
R6750	T23669	T23668	dobj	mice,produce
R6751	T23670	T23671	dep	that,express
R6752	T23671	T23669	relcl	express,mice
R6753	T23672	T23673	amod	human,E2
R6754	T23673	T23671	dobj	E2,express
R6755	T23674	T23663	punct	.,strategy
R6756	T23676	T23677	nmod	LAP,tTA
R6757	T23677	T23679	nmod	tTA,mice
R6758	T23678	T23677	punct	-,tTA
R6759	T23679	T23681	nsubjpass	mice,described
R6760	T23680	T23679	amod	transgenic,mice
R6761	T23682	T23681	aux	have,described
R6762	T23683	T23681	auxpass	been,described
R6763	T23684	T23681	advmod	previously,described
R6764	T23685	T23686	punct	[,34
R6765	T23686	T23681	parataxis	34,described
R6766	T23687	T23686	punct	],34
R6767	T23688	T23681	punct	.,described
R6768	T23690	T23691	det	These,mice
R6769	T23691	T23692	nsubj	mice,express
R6770	T23693	T23694	det	the,transactivator
R6771	T23694	T23692	dobj	transactivator,express
R6772	T23695	T23696	npadvmod	tetracycline,controlled
R6773	T23696	T23694	amod	controlled,transactivator
R6774	T23697	T23696	punct	-,controlled
R6775	T23698	T23694	punct	(,transactivator
R6776	T23699	T23694	appos	tTA,transactivator
R6777	T23700	T23694	punct	),transactivator
R6778	T23701	T23694	prep	from,transactivator
R6779	T23702	T23703	det	the,promoter
R6780	T23703	T23701	pobj	promoter,from
R6781	T23704	T23705	npadvmod	liver,specific
R6782	T23705	T23703	amod	specific,promoter
R6783	T23706	T23703	compound	LAP,promoter
R6784	T23707	T23692	punct	.,express
R6785	T23709	T23710	det	The,transgene
R6786	T23710	T23714	nsubj	transgene,contains
R6787	T23711	T23712	compound	TRE,E2
R6788	T23712	T23710	compound	E2,transgene
R6789	T23713	T23712	punct	-,E2
R6790	T23715	T23716	det	the,element
R6791	T23716	T23714	dobj	element,contains
R6792	T23717	T23716	compound	tetracycline,element
R6793	T23718	T23716	compound	response,element
R6794	T23719	T23716	punct	(,element
R6795	T23720	T23716	appos	TRE,element
R6796	T23721	T23716	punct	),element
R6797	T23722	T23716	prep	as,element
R6798	T23723	T23722	pobj	part,as
R6799	T23724	T23723	prep	of,part
R6800	T23725	T23726	det	the,promoter
R6801	T23726	T23724	pobj	promoter,of
R6802	T23727	T23716	punct	", ",element
R6803	T23728	T23729	det	a,intron
R6804	T23729	T23716	conj	intron,element
R6805	T23730	T23729	amod	synthetic,intron
R6806	T23731	T23732	punct	(,line
R6807	T23732	T23729	parataxis	line,intron
R6808	T23733	T23732	amod	thin,line
R6809	T23734	T23732	punct	),line
R6810	T23735	T23729	punct	", ",intron
R6811	T23736	T23737	det	the,cDNA
R6812	T23737	T23729	conj	cDNA,intron
R6813	T23738	T23737	amod	human,cDNA
R6814	T23739	T23737	compound	E2,cDNA
R6815	T23740	T23737	punct	", ",cDNA
R6816	T23741	T23742	det	an,spacer
R6817	T23742	T23737	conj	spacer,cDNA
R6818	T23743	T23742	compound	alanine,spacer
R6819	T23744	T23742	punct	", ",spacer
R6820	T23745	T23746	det	a,tag
R6821	T23746	T23742	conj	tag,spacer
R6822	T23747	T23748	compound	c,myc
R6823	T23748	T23746	compound	myc,tag
R6824	T23749	T23748	punct	-,myc
R6825	T23750	T23746	compound	epitope,tag
R6826	T23751	T23746	punct	", ",tag
R6827	T23752	T23746	cc	and,tag
R6828	T23753	T23754	npadvmod	SV40,derived
R6829	T23754	T23755	amod	derived,sequence
R6830	T23755	T23746	conj	sequence,tag
R6831	T23756	T23755	compound	polyadenylation,sequence
R6832	T23757	T23714	punct	.,contains
R6833	T23759	T23760	det	This,construct
R6834	T23760	T23761	nsubjpass	construct,used
R6835	T23762	T23761	auxpass	was,used
R6836	T23763	T23764	aux	to,create
R6837	T23764	T23761	advcl	create,used
R6838	T23765	T23766	amod	several,lines
R6839	T23766	T23764	dobj	lines,create
R6840	T23767	T23766	prep	of,lines
R6841	T23768	T23769	amod	transgenic,mice
R6842	T23769	T23767	pobj	mice,of
R6843	T23770	T23761	punct	.,used
R6844	T23772	T23773	meta	B,analysis
R6845	T23774	T23773	punct	", ",analysis
R6846	T23775	T23776	compound	Western,blot
R6847	T23776	T23773	compound	blot,analysis
R6848	T23777	T23773	prep	of,analysis
R6849	T23778	T23779	compound	E2,protein
R6850	T23779	T23777	pobj	protein,of
R6851	T23780	T23779	prep	in,protein
R6852	T23781	T23780	pobj	liver,in
R6853	T23782	T23781	prep	of,liver
R6854	T23783	T23784	nmod	control,mice
R6855	T23784	T23782	pobj	mice,of
R6856	T23785	T23783	cc	and,control
R6857	T23786	T23783	conj	intermediate,control
R6858	T23787	T23784	compound	MSUD,mice
R6859	T23788	T23773	punct	.,analysis
R6860	T23791	T23792	mark	that,was
R6861	T23792	T23790	ccomp	was,Note
R6862	T23793	T23794	det	the,amount
R6863	T23794	T23792	nsubj	amount,was
R6864	T23795	T23794	prep	of,amount
R6865	T23796	T23797	amod	human,protein
R6866	T23797	T23795	pobj	protein,of
R6867	T23798	T23797	compound	E2,protein
R6868	T23799	T23800	punct	(,MW
R6869	T23800	T23797	parataxis	MW,protein
R6870	T23801	T23800	amod	predicted,MW
R6871	T23802	T23803	punct	~,54
R6872	T23803	T23804	nummod	54,Kd
R6873	T23804	T23800	appos	Kd,MW
R6874	T23805	T23800	punct	),MW
R6875	T23806	T23794	prep	in,amount
R6876	T23807	T23806	pobj	mice,in
R6877	T23808	T23807	prep	from,mice
R6878	T23809	T23810	compound	Lines,A
R6879	T23810	T23808	pobj	A,from
R6880	T23811	T23810	cc	and,A
R6881	T23812	T23813	nummod	525,A
R6882	T23813	T23810	conj	A,A
R6883	T23814	T23792	acomp	variable,was
R6884	T23815	T23792	cc	but,was
R6885	T23816	T23817	prep	in,was
R6886	T23817	T23792	conj	was,was
R6887	T23818	T23816	pobj	many,in
R6888	T23819	T23818	prep	of,many
R6889	T23820	T23821	det	the,animals
R6890	T23821	T23819	pobj	animals,of
R6891	T23822	T23823	det	the,amount
R6892	T23823	T23817	nsubj	amount,was
R6893	T23824	T23817	acomp	similar,was
R6894	T23825	T23824	prep	to,similar
R6895	T23826	T23827	det	the,amount
R6896	T23827	T23825	pobj	amount,to
R6897	T23828	T23827	prep	of,amount
R6898	T23829	T23830	compound	mouse,E2
R6899	T23830	T23828	pobj	E2,of
R6900	T23831	T23832	punct	(,Kd
R6901	T23832	T23830	parataxis	Kd,E2
R6902	T23833	T23832	nsubj	MW,Kd
R6903	T23834	T23832	punct	=,Kd
R6904	T23835	T23836	punct	~,47
R6905	T23836	T23832	nummod	47,Kd
R6906	T23837	T23832	punct	),Kd
R6907	T23838	T23827	prep	in,amount
R6908	T23839	T23840	compound	control,animals
R6909	T23840	T23838	pobj	animals,in
R6910	T23841	T23790	punct	.,Note
R6911	T23843	T23844	compound	Re,probing
R6912	T23844	T23846	nsubj	probing,confirmed
R6913	T23845	T23844	punct	-,probing
R6914	T23847	T23844	prep	with,probing
R6915	T23848	T23849	det	a,antibody
R6916	T23849	T23847	pobj	antibody,with
R6917	T23850	T23851	compound	c,myc
R6918	T23851	T23849	compound	myc,antibody
R6919	T23852	T23851	punct	-,myc
R6920	T23853	T23849	compound	tag,antibody
R6921	T23854	T23855	det	the,presence
R6922	T23855	T23846	dobj	presence,confirmed
R6923	T23856	T23855	prep	of,presence
R6924	T23857	T23858	det	the,E2
R6925	T23858	T23856	pobj	E2,of
R6926	T23859	T23860	npadvmod	transgene,derived
R6927	T23860	T23858	amod	derived,E2
R6928	T23861	T23858	punct	", ",E2
R6929	T23862	T23863	compound	c,myc
R6930	T23863	T23865	npadvmod	myc,tagged
R6931	T23864	T23863	punct	-,myc
R6932	T23865	T23858	amod	tagged,E2
R6933	T23866	T23858	punct	", ",E2
R6934	T23867	T23858	amod	human,E2
R6935	T23868	T23846	prep	in,confirmed
R6936	T23869	T23870	amod	transgenic,mice
R6937	T23870	T23868	pobj	mice,in
R6938	T23871	T23868	cc	but,in
R6939	T23872	T23871	neg	not,but
R6940	T23873	T23868	conj	in,in
R6941	T23874	T23873	pobj	controls,in
R6942	T23875	T23846	punct	.,confirmed
R6943	T23877	T23878	compound	Western,blot
R6944	T23878	T23879	compound	blot,analysis
R6945	T23879	T23880	nsubj	analysis,revealed
R6946	T23881	T23879	prep	of,analysis
R6947	T23882	T23881	pobj	brain,of
R6948	T23883	T23884	punct	(,C
R6949	T23884	T23882	parataxis	C,brain
R6950	T23885	T23884	punct	),C
R6951	T23886	T23882	punct	", ",brain
R6952	T23887	T23882	conj	kidney,brain
R6953	T23888	T23889	punct	(,D
R6954	T23889	T23887	parataxis	D,kidney
R6955	T23890	T23889	punct	),D
R6956	T23891	T23887	punct	", ",kidney
R6957	T23892	T23887	cc	and,kidney
R6958	T23893	T23887	conj	muscle,kidney
R6959	T23894	T23895	punct	(,E
R6960	T23895	T23893	parataxis	E,muscle
R6961	T23896	T23895	punct	),E
R6962	T23897	T23898	amod	negligible,amounts
R6963	T23898	T23880	dobj	amounts,revealed
R6964	T23899	T23898	prep	of,amounts
R6965	T23900	T23901	npadvmod	transgene,derived
R6966	T23901	T23902	amod	derived,E2
R6967	T23902	T23899	pobj	E2,of
R6968	T23903	T23880	prep	in,revealed
R6969	T23904	T23905	det	those,tissues
R6970	T23905	T23903	pobj	tissues,in
R6971	T23906	T23880	punct	.,revealed
R6972	T23908	T23909	det	All,blots
R6973	T23909	T23910	nsubjpass	blots,re-probed
R6974	T23911	T23910	auxpass	were,re-probed
R6975	T23912	T23910	prep	with,re-probed
R6976	T23913	T23914	det	an,antibody
R6977	T23914	T23912	pobj	antibody,with
R6978	T23915	T23914	compound	actin,antibody
R6979	T23916	T23917	aux	to,allow
R6980	T23917	T23910	advcl	allow,re-probed
R6981	T23918	T23919	nsubjpass	amount,compared
R6982	T23919	T23917	ccomp	compared,allow
R6983	T23920	T23918	prep	of,amount
R6984	T23921	T23920	pobj	protein,of
R6985	T23922	T23921	acl	loaded,protein
R6986	T23923	T23922	prep	in,loaded
R6987	T23924	T23925	det	each,lane
R6988	T23925	T23923	pobj	lane,in
R6989	T23926	T23919	aux	to,compared
R6990	T23927	T23919	auxpass	be,compared
R6991	T23928	T23910	punct	.,re-probed
R6992	T24446	T24445	prep	of,Characterization
R6993	T24447	T24448	det	the,model
R6994	T24448	T24446	pobj	model,of
R6995	T24449	T24448	amod	intermediate,model
R6996	T24450	T24448	nmod	MSUD,model
R6997	T24451	T24448	amod	murine,model
R6998	T24452	T24445	punct	.,Characterization
R6999	T24454	T24455	meta	A,analysis
R7000	T24456	T24455	punct	", ",analysis
R7001	T24457	T24455	compound	Survival,analysis
R7002	T24458	T24455	prep	of,analysis
R7003	T24459	T24460	amod	transgenic,lines
R7004	T24460	T24458	pobj	lines,of
R7005	T24461	T24460	compound	rescue,lines
R7006	T24462	T24455	punct	.,analysis
R7007	T24464	T24465	dep	Presented,are
R7008	T24466	T24465	nsubj	percentages,are
R7009	T24467	T24466	prep	of,percentages
R7010	T24468	T24467	pobj	mice,of
R7011	T24469	T24468	amod	alive,mice
R7012	T24470	T24469	prep	at,alive
R7013	T24471	T24470	pobj	weaning,at
R7014	T24472	T24468	prep	from,mice
R7015	T24473	T24474	det	each,line
R7016	T24474	T24472	pobj	line,from
R7017	T24475	T24474	amod	transgenic,line
R7018	T24476	T24474	acl	tested,line
R7019	T24477	T24478	dep	that,had
R7020	T24478	T24476	ccomp	had,tested
R7021	T24479	T24480	det	the,genotype
R7022	T24480	T24478	dobj	genotype,had
R7023	T24481	T24480	compound	rescue,genotype
R7024	T24482	T24483	punct	(,homozygous
R7025	T24483	T24478	parataxis	homozygous,had
R7026	T24484	T24483	advmod	i.e.,homozygous
R7027	T24485	T24483	punct	", ",homozygous
R7028	T24486	T24483	prep	for,homozygous
R7029	T24487	T24486	pobj	knockout,for
R7030	T24488	T24487	prep	of,knockout
R7031	T24489	T24490	amod	endogenous,E2
R7032	T24490	T24488	pobj	E2,of
R7033	T24491	T24483	cc	and,homozygous
R7034	T24492	T24483	conj	positive,homozygous
R7035	T24493	T24492	prep	for,positive
R7036	T24494	T24495	preconj	both,transgenes
R7037	T24495	T24493	pobj	transgenes,for
R7038	T24496	T24495	det	the,transgenes
R7039	T24497	T24498	nmod	LAP,tTA
R7040	T24498	T24495	nmod	tTA,transgenes
R7041	T24499	T24498	punct	-,tTA
R7042	T24500	T24498	cc	and,tTA
R7043	T24501	T24502	compound	TRE,E2
R7044	T24502	T24498	conj	E2,tTA
R7045	T24503	T24502	punct	-,E2
R7046	T24504	T24483	punct	),homozygous
R7047	T24505	T24465	punct	.,are
R7048	T24507	T24508	advmod	Also,is
R7049	T24509	T24508	dep	plotted,is
R7050	T24510	T24511	det	the,frequency
R7051	T24511	T24508	nsubj	frequency,is
R7052	T24512	T24511	amod	theoretical,frequency
R7053	T24513	T24511	amod	maximum,frequency
R7054	T24514	T24515	prep	at,expected
R7055	T24515	T24511	relcl	expected,frequency
R7056	T24516	T24514	pobj	which,at
R7057	T24517	T24518	det	this,genotype
R7058	T24518	T24515	nsubjpass	genotype,expected
R7059	T24519	T24515	auxpass	is,expected
R7060	T24520	T24515	prep	in,expected
R7061	T24521	T24522	det	this,population
R7062	T24522	T24520	pobj	population,in
R7063	T24523	T24522	prep	of,population
R7064	T24524	T24523	pobj	animals,of
R7065	T24525	T24508	punct	.,is
R7066	T24527	T24528	det	The,numbers
R7067	T24528	T24529	nsubj	numbers,indicates
R7068	T24530	T24528	prep	on,numbers
R7069	T24531	T24532	det	the,bars
R7070	T24532	T24530	pobj	bars,on
R7071	T24533	T24534	det	the,numbers
R7072	T24534	T24529	dobj	numbers,indicates
R7073	T24535	T24534	prep	of,numbers
R7074	T24536	T24535	pobj	observations,of
R7075	T24537	T24534	prep	for,numbers
R7076	T24538	T24539	det	each,line
R7077	T24539	T24537	pobj	line,for
R7078	T24540	T24529	punct	.,indicates
R7079	T24542	T24543	meta	B,Ratio
R7080	T24544	T24543	punct	", ",Ratio
R7081	T24545	T24543	prep	of,Ratio
R7082	T24546	T24547	amod	total,BCAA
R7083	T24547	T24545	pobj	BCAA,of
R7084	T24548	T24543	prep	to,Ratio
R7085	T24549	T24548	pobj	alanine,to
R7086	T24550	T24543	prep	in,Ratio
R7087	T24551	T24550	pobj	blood,in
R7088	T24552	T24551	prep	of,blood
R7089	T24553	T24552	pobj	mice,of
R7090	T24554	T24543	prep	from,Ratio
R7091	T24555	T24554	pobj	controls,from
R7092	T24556	T24555	cc	and,controls
R7093	T24557	T24558	compound	Lines,A
R7094	T24558	T24555	conj	A,controls
R7095	T24559	T24558	cc	and,A
R7096	T24560	T24558	conj	525A,A
R7097	T24561	T24543	punct	.,Ratio
R7098	T24563	T24564	amod	Total,BCAA
R7099	T24564	T24565	nsubj	BCAA,represent
R7100	T24566	T24567	det	the,sum
R7101	T24567	T24565	dobj	sum,represent
R7102	T24568	T24567	prep	of,sum
R7103	T24569	T24568	pobj	leucine,of
R7104	T24570	T24569	punct	", ",leucine
R7105	T24571	T24569	conj	isoleucine,leucine
R7106	T24572	T24571	punct	", ",isoleucine
R7107	T24573	T24571	cc	and,isoleucine
R7108	T24574	T24571	conj	valine,isoleucine
R7109	T24575	T24565	punct	.,represent
R7110	T24577	T24578	compound	BCAA,alanine
R7111	T24578	T24580	compound	alanine,values
R7112	T24579	T24578	punct	/,alanine
R7113	T24580	T24581	nsubj	values,were
R7114	T24582	T24583	advmod	significantly,greater
R7115	T24583	T24581	acomp	greater,were
R7116	T24584	T24583	prep	for,greater
R7117	T24585	T24586	compound	Lines,A
R7118	T24586	T24584	pobj	A,for
R7119	T24587	T24586	cc	and,A
R7120	T24588	T24586	conj	525A,A
R7121	T24589	T24583	prep	compared,greater
R7122	T24590	T24589	prep	to,compared
R7123	T24591	T24590	pobj	controls,to
R7124	T24592	T24581	prep	at,were
R7125	T24593	T24594	det	all,ages
R7126	T24594	T24592	pobj	ages,at
R7127	T24595	T24594	acl	tested,ages
R7128	T24596	T24581	punct	.,were
R7129	T24598	T24599	det	The,numbers
R7130	T24599	T24600	nsubj	numbers,indicates
R7131	T24601	T24599	prep	on,numbers
R7132	T24602	T24603	det	the,bars
R7133	T24603	T24601	pobj	bars,on
R7134	T24604	T24605	det	the,numbers
R7135	T24605	T24600	dobj	numbers,indicates
R7136	T24606	T24605	prep	of,numbers
R7137	T24607	T24606	pobj	samples,of
R7138	T24608	T24607	acl	analyzed,samples
R7139	T24609	T24600	punct	.,indicates
R7140	T24611	T24612	punct	*,0.01
R7141	T24612	T24616	ccomp	0.01,0.005
R7142	T24613	T24612	punct	", ",0.01
R7143	T24614	T24612	nsubj	P,0.01
R7144	T24615	T24612	punct	≤,0.01
R7145	T24617	T24616	punct	;,0.005
R7146	T24618	T24616	punct	**,0.005
R7147	T24619	T24616	punct	", ",0.005
R7148	T24620	T24616	nsubj	P,0.005
R7149	T24621	T24616	punct	<,0.005
R7150	T24622	T24616	punct	.,0.005
R7151	T24624	T24625	meta	C,activity
R7152	T24626	T24625	punct	", ",activity
R7153	T24627	T24625	compound	BCKDH,activity
R7154	T24628	T24625	compound	enzyme,activity
R7155	T24629	T24625	prep	in,activity
R7156	T24630	T24629	pobj	liver,in
R7157	T24631	T24630	prep	of,liver
R7158	T24632	T24633	nmod	control,mice
R7159	T24633	T24631	pobj	mice,of
R7160	T24634	T24632	cc	and,control
R7161	T24635	T24636	compound	Lines,A
R7162	T24636	T24632	conj	A,control
R7163	T24637	T24636	cc	and,A
R7164	T24638	T24636	conj	525A,A
R7165	T24639	T24625	punct	.,activity
R7166	T24641	T24642	punct	*,0.001
R7167	T24642	T24646	ccomp	0.001,0.0001
R7168	T24643	T24642	punct	", ",0.001
R7169	T24644	T24642	nsubj	P,0.001
R7170	T24645	T24642	punct	≤,0.001
R7171	T24647	T24646	punct	;,0.0001
R7172	T24648	T24646	punct	**,0.0001
R7173	T24649	T24646	punct	", ",0.0001
R7174	T24650	T24646	nsubj	P,0.0001
R7175	T24651	T24646	punct	≤,0.0001
R7176	T24652	T24646	punct	.,0.0001
R7177	T24654	T24655	meta	D,curves
R7178	T24656	T24655	punct	", ",curves
R7179	T24657	T24655	compound	Survival,curves
R7180	T24658	T24655	prep	for,curves
R7181	T24659	T24658	pobj	mice,for
R7182	T24660	T24659	prep	from,mice
R7183	T24661	T24662	compound	Lines,A
R7184	T24662	T24660	pobj	A,from
R7185	T24663	T24662	cc	and,A
R7186	T24664	T24662	conj	525A,A
R7187	T24665	T24655	punct	.,curves
R7188	T24667	T24668	compound	Rescue,mice
R7189	T24668	T24669	nsubjpass	mice,monitored
R7190	T24670	T24668	amod	alive,mice
R7191	T24671	T24670	prep	at,alive
R7192	T24672	T24671	pobj	weaning,at
R7193	T24673	T24669	auxpass	were,monitored
R7194	T24674	T24675	mark	until,were
R7195	T24675	T24669	advcl	were,monitored
R7196	T24676	T24675	nsubj	they,were
R7197	T24677	T24675	acomp	moribund,were
R7198	T24678	T24677	cc	and,moribund
R7199	T24679	T24680	advmod	subsequently,sacrificed
R7200	T24680	T24677	conj	sacrificed,moribund
R7201	T24681	T24675	cc	or,were
R7202	T24682	T24683	auxpass	were,found
R7203	T24683	T24675	conj	found,were
R7204	T24684	T24683	advcl	dead,found
R7205	T24685	T24683	prep	in,found
R7206	T24686	T24687	poss	their,cages
R7207	T24687	T24685	pobj	cages,in
R7208	T24688	T24669	punct	.,monitored
R7247	T24915	T24914	prep	of,Summary
R7248	T24916	T24917	amod	additional,levels
R7249	T24917	T24915	pobj	levels,of
R7250	T24918	T24917	compound	blood,levels
R7251	T24919	T24920	compound	amino,acid
R7252	T24920	T24917	compound	acid,levels
R7253	T24921	T24917	prep	in,levels
R7254	T24922	T24923	det	the,model
R7255	T24923	T24921	pobj	model,in
R7256	T24924	T24923	amod	intermediate,model
R7257	T24925	T24923	compound	MSUD,model
R7258	T24926	T24923	cc	and,model
R7259	T24927	T24928	compound	littermate,controls
R7260	T24928	T24923	conj	controls,model
R7261	T24929	T24930	mark	as,determined
R7262	T24930	T24917	advcl	determined,levels
R7263	T24931	T24930	prep	by,determined
R7264	T24932	T24933	amod	tandem,spectrometry
R7265	T24933	T24931	pobj	spectrometry,by
R7266	T24934	T24933	compound	mass,spectrometry
R7267	T24935	T24914	punct	.,Summary
R7268	T24937	T24938	nsubjpass	Samples,collected
R7269	T24939	T24938	auxpass	were,collected
R7270	T24940	T24938	prep	from,collected
R7271	T24941	T24940	pobj	mice,from
R7272	T24942	T24943	dep	that,were
R7273	T24943	T24941	relcl	were,mice
R7274	T24944	T24945	quantmod	4,6
R7275	T24945	T24947	nummod	6,weeks
R7276	T24946	T24945	punct	–,6
R7277	T24947	T24943	attr	weeks,were
R7278	T24948	T24947	prep	of,weeks
R7279	T24949	T24948	pobj	age,of
R7280	T24950	T24938	punct	.,collected
R7281	T24952	T24953	det	All,values
R7282	T24953	T24954	nsubj	values,are
R7283	T24955	T24954	attr	mean,are
R7284	T24956	T24955	cc	+,mean
R7285	T24957	T24956	punct	/,+
R7286	T24958	T24956	punct	-,+
R7287	T24959	T24955	conj	SEM,mean
R7288	T24960	T24954	punct	.,are
R3928	T15827	T15828	det	The,transgene
R3929	T15828	T15832	nsubj	transgene,directs
R3930	T15829	T15830	compound	LAP,tTA
R3931	T15830	T15828	compound	tTA,transgene
R3932	T15831	T15830	punct	-,tTA
R3933	T15833	T15834	punct	[,34
R3934	T15834	T15828	parataxis	34,transgene
R3935	T15835	T15834	punct	],34
R3936	T15836	T15837	amod	high,levels
R3937	T15837	T15832	dobj	levels,directs
R3938	T15838	T15837	prep	of,levels
R3939	T15839	T15840	npadvmod	liver,specific
R3940	T15840	T15841	amod	specific,expression
R3941	T15841	T15838	pobj	expression,of
R3942	T15842	T15841	prep	of,expression
R3943	T15843	T15844	det	the,transactivator
R3993	T15897	T15898	nmod	LAP,tTA
R3994	T15898	T15900	nmod	tTA,mice
R3995	T15899	T15898	punct	-,tTA
R3996	T15903	T15902	auxpass	w,e
R3997	T15904	T15902	advcl	re,e
R3998	T15919	T15920	amod	c,e
R3999	T15923	T15922	cc	and,d
R4000	T15924	T15922	conj	c,d
R4001	T15930	T15929	acl	a,r
R4002	T15932	T15928	aux	t,r
R4003	T15933	T15928	auxpass	e,r
R4004	T15937	T15936	agent	by,zed
R4005	T15938	T15939	det	the,laboratory
R4006	T15939	T15937	pobj	laboratory,by
R4007	T15940	T15939	compound	Bujard,laboratory
R4291	T16290	T16289	pcomp	rescuing,at
R4292	T16291	T16292	det	the,phenotype
R4294	T16292	T16290	dobj	phenotype,rescuing
R4295	T16293	T16292	amod	neonatal,phenotype
R4296	T16294	T16292	amod	lethal,phenotype
R4298	T16295	T16292	prep	of,phenotype
R4299	T16296	T16297	det	the,knockout
R4300	T16297	T16295	pobj	knockout,of
R4301	T16298	T16297	compound	E2,knockout
R4303	T16299	T16280	punct	.,appears
R4304	T16301	T16302	prep	In,failed
R4306	T16303	T16301	pobj	contrast,In
R4307	T16304	T16302	punct	", ",failed
R4308	T16305	T16306	compound	line,520B
R4310	T16306	T16302	nsubj	520B,failed
R4312	T16307	T16302	advmod	completely,failed
R4313	T16308	T16309	aux	to,rescue
R4314	T16309	T16302	xcomp	rescue,failed
R4315	T16310	T16311	det	the,phenotype
R4317	T16311	T16309	dobj	phenotype,rescue
R4319	T16312	T16311	amod	neonatal,phenotype
R4320	T16313	T16311	amod	lethal,phenotype
R4321	T16314	T16302	punct	.,failed
R4323	T16316	T16317	det	All,lines
R4324	T16317	T16319	nsubj	lines,produced
R4325	T16318	T16317	amod	other,lines
R4327	T16320	T16317	acl	tested,lines
R4329	T16321	T16322	amod	surviving,animals
R4330	T16322	T16319	dobj	animals,produced
R4331	T16323	T16322	compound	rescue,animals
R4333	T16324	T16319	prep	at,produced
R4334	T16325	T16326	det	a,frequency
R4335	T16326	T16324	pobj	frequency,at
R4337	T16327	T16326	prep	between,frequency
R4338	T16328	T16329	nummod	1,%
R4339	T16329	T16327	pobj	%,between
R4341	T16330	T16328	cc	and,1
R4342	T16331	T16328	conj	5,1
R4343	T16332	T16329	prep	of,%
R4353	T16341	T16340	nsubj	n,i
R4360	T16352	T16353	advmod	s,e
R4361	T16353	T16351	acomp	e,were
R4372	T16363	T16362	cc	d,or
R4369	T16380	T16381	compound	Fig.,4B
R4370	T16381	T16382	nsubj	4B,shows
R4371	T16383	T16384	det	the,results
R4373	T16384	T16382	dobj	results,shows
R4374	T16385	T16384	prep	of,results
R4375	T16367	T16366	pobj	ai,t
R4376	T16386	T16387	compound	blood,analysis
R4377	T16371	T16370	conj	c,d
R4378	T16387	T16385	pobj	analysis,of
R4379	T16372	T16373	poss	h,a
R4380	T16388	T16389	compound	amino,acid
R4381	T16389	T16387	compound	acid,analysis
R4382	T16376	T16377	amod	a,cterization
R4383	T16390	T16384	acl	presented,results
R4384	T16391	T16390	prep	as,presented
R4385	T16392	T16393	det	a,ratio
R4386	T16393	T16391	pobj	ratio,as
R4387	T16394	T16393	prep	of,ratio
R4389	T16395	T16396	amod	total,BCAA
R4392	T16396	T16394	pobj	BCAA,of
R4396	T16397	T16393	prep	to,ratio
R4400	T16398	T16397	pobj	alanine,to
R4533	T16440	T16439	pobj	controls,between
R4537	T16441	T16440	cc	and,controls
R4540	T16442	T16440	conj	knockouts,controls
R4544	T16443	T16444	punct	(,see
R4548	T16444	T16428	parataxis	see,are
R4551	T16445	T16446	compound	Figure,2C
R4552	T16446	T16444	dobj	2C,see
R4553	T16447	T16444	punct	),see
R4556	T16448	T16426	punct	.,Note
R4563	T16450	T16451	compound	Blood,levels
R4565	T16451	T16452	nsubjpass	levels,reduced
R4568	T16453	T16451	prep	of,levels
R4574	T16454	T16453	pobj	alanine,of
R4577	T16455	T16454	punct	", ",alanine
R4580	T16456	T16454	conj	glutamate,alanine
R4583	T16457	T16456	punct	", ",glutamate
R4586	T16458	T16456	cc	and,glutamate
R4590	T16459	T16456	conj	glutamine,glutamate
R4593	T16460	T16452	auxpass	were,reduced
R4597	T16461	T16452	prep	in,reduced
R4601	T16462	T16463	compound	Line,A
R4605	T16463	T16464	compound	A,mice
R4609	T16464	T16461	pobj	mice,in
R4616	T16465	T16452	punct	", ",reduced
R4620	T16466	T16467	mark	as,was
R4623	T16467	T16452	advcl	was,reduced
R4626	T16468	T16467	nsubj	glutamate,was
R4629	T16469	T16468	prep	in,glutamate
R4633	T16470	T16471	compound	Line,525A
R4636	T16471	T16472	compound	525A,mice
R4640	T16472	T16469	pobj	mice,in
R4644	T16473	T16468	punct	", ",glutamate
R4648	T16474	T16468	prep	compared,glutamate
R4652	T16475	T16474	prep	to,compared
R4656	T16476	T16475	pobj	controls,to
R4658	T16477	T16478	punct	(,Table
R4661	T16478	T16467	parataxis	Table,was
R4667	T16479	T16478	nummod	2,Table
R4671	T16480	T16478	punct	),Table
R4672	T16481	T16452	punct	.,reduced
R4388	T16484	T16485	mark	Because,demonstrated
R4390	T16485	T16503	advcl	demonstrated,examined
R4391	T16486	T16487	det	the,mice
R4393	T16487	T16485	nsubjpass	mice,demonstrated
R4394	T16488	T16489	compound	LAP,tTA
R4395	T16489	T16487	compound	tTA,mice
R4397	T16490	T16489	punct	-,tTA
R4398	T16491	T16492	dep	that,used
R4399	T16492	T16487	relcl	used,mice
R4401	T16493	T16492	auxpass	were,used
R4402	T16494	T16495	aux	to,drive
R4403	T16495	T16492	advcl	drive,used
R4404	T16496	T16495	dobj	expression,drive
R4405	T16497	T16496	prep	of,expression
R4407	T16498	T16499	amod	transgenic,E2
R4408	T16499	T16497	pobj	E2,of
R4409	T16500	T16499	amod	human,E2
R4411	T16501	T16485	aux	have,demonstrated
R4415	T16502	T16485	auxpass	been,demonstrated
R4417	T16504	T16505	aux	to,produce
R4418	T16505	T16485	xcomp	produce,demonstrated
R4419	T16506	T16507	npadvmod	liver,specific
R4421	T16507	T16508	amod	specific,expression
R4422	T16508	T16505	dobj	expression,produce
R4423	T16509	T16510	punct	[,34
R4425	T16510	T16485	parataxis	34,demonstrated
R4426	T16511	T16510	punct	],34
R4427	T16512	T16503	punct	", ",examined
R4429	T16513	T16514	compound	BCKDH,activity
R4430	T16514	T16503	nsubjpass	activity,examined
R4431	T16515	T16514	compound	enzyme,activity
R4433	T16516	T16514	cc	and,activity
R4434	T16517	T16514	conj	production,activity
R4435	T16518	T16517	prep	of,production
R4437	T16519	T16520	amod	human,protein
R4438	T16520	T16518	pobj	protein,of

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T24441	5319-5323	NCBITaxon:10088	denotes	mice
T24444	5433-5443	GO_EXT:killing	denotes	sacrificed
T24443	5354-5358	NCBITaxon:10088	denotes	mice
T24442	5347-5353	SO_EXT:sequence_rescue_process	denotes	Rescue
T24913	6229-6233	NCBITaxon:10088	denotes	mice
T24912	6085-6095	CHEBI_SO_EXT:amino_acid	denotes	amino acid
T24911	6079-6084	UBERON:0000178	denotes	blood
T24440	5260-5264	NCBITaxon:10088	denotes	mice
T24439	5222-5227	UBERON:0002107	denotes	liver
T24438	5203-5209	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T24437	4995-5002	CHEBI_SO_EXT:alanine	denotes	alanine
T24436	4990-4994	CHEBI:22918	denotes	BCAA
T24435	4982-4988	CHEBI_SO_EXT:valine	denotes	valine
T24434	4966-4976	CHEBI_SO_EXT:isoleucine	denotes	isoleucine
T24433	4957-4964	CHEBI_SO_EXT:leucine	denotes	leucine
T24432	4931-4935	CHEBI:22918	denotes	BCAA
T24431	4884-4888	NCBITaxon:10088	denotes	mice
T24430	4875-4880	UBERON:0000178	denotes	blood
T24429	4864-4871	CHEBI_SO_EXT:alanine	denotes	alanine
T24428	4856-4860	CHEBI:22918	denotes	BCAA
T24427	4752-4759	NCBITaxon:33208	denotes	animals
T24426	4709-4717	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T24425	4632-4642	SO_EXT:0000902	denotes	transgenes
T24424	4629-4631	PR_EXT:000006300	denotes	E2
T24423	4613-4616	PR_EXT:000005308	denotes	LAP
T24422	4584-4586	PR_EXT:000006300	denotes	E2
T24421	4530-4538	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T24420	4523-4529	SO_EXT:sequence_rescue_process	denotes	rescue
T24419	4487-4497	SO_EXT:transgenic_entity	denotes	transgenic
T24418	4455-4459	NCBITaxon:10088	denotes	mice
T24417	4412-4418	SO_EXT:sequence_rescue_process	denotes	rescue
T24416	4401-4411	SO_EXT:transgenic_entity	denotes	transgenic
T24415	4363-4369	NCBITaxon:10088	denotes	murine
T23645	1906-1910	NCBITaxon:10088	denotes	mice
T23644	1895-1905	SO_EXT:transgenic_entity	denotes	transgenic
T23643	1889-1891	PR_EXT:000006300	denotes	E2
T23642	1883-1888	NCBITaxon:9606	denotes	human
T23641	1875-1881	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	tagged
T23640	1869-1874	PR_EXT:000000084	denotes	c-myc
T23639	1850-1859	SO_EXT:0000902	denotes	transgene
T23638	1811-1819	GO:0042571	denotes	antibody
T23637	1807-1810	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	tag
T23636	1801-1806	PR_EXT:000000084	denotes	c-myc
T23635	1774-1781	NCBITaxon:33208	denotes	animals
T23634	1748-1750	PR_EXT:000006300	denotes	E2
T23633	1742-1747	NCBITaxon:10088	denotes	mouse
T23632	1694-1701	NCBITaxon:33208	denotes	animals
T23631	1634-1638	NCBITaxon:10088	denotes	mice
T23630	1601-1608	CHEBI_PR_EXT:protein	denotes	protein
T23629	1598-1600	PR_EXT:000006300	denotes	E2
T23628	1592-1597	NCBITaxon:9606	denotes	human
T23627	1562-1566	NCBITaxon:10088	denotes	mice
T23626	1523-1528	UBERON:0002107	denotes	liver
T23625	1512-1519	CHEBI_PR_EXT:protein	denotes	protein
T23624	1509-1511	PR_EXT:000006300	denotes	E2
T23623	1475-1479	NCBITaxon:10088	denotes	mice
T23622	1464-1474	SO_EXT:transgenic_entity	denotes	transgenic
T23621	1418-1427	SO_EXT:engineered_biological_sequence	denotes	construct
T23620	1387-1411	SO_EXT:0000610	denotes	polyadenylation sequence
T23619	1387-1402	GO:0043631	denotes	polyadenylation
T23618	1374-1378	NCBITaxon:10633	denotes	SV40
T23617	1365-1368	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	tag
T23616	1357-1364	CHEBI_SO_EXT:epitope	denotes	epitope
T23615	1351-1356	PR_EXT:000000084	denotes	c-myc
T23614	1333-1340	CHEBI_SO_EXT:alanine	denotes	alanine
T23613	1324-1328	SO_EXT:cDNA	denotes	cDNA
T23612	1321-1323	PR_EXT:000006300	denotes	E2
T23611	1315-1320	NCBITaxon:9606	denotes	human
T23610	1291-1297	SO_EXT:0000188	denotes	intron
T23609	1269-1277	SO_EXT:0000167	denotes	promoter
T23608	1231-1239	GO_EXT:reaction_or_response	denotes	response
T23607	1218-1230	CHEBI:27902	denotes	tetracycline
T23606	1195-1204	SO_EXT:0000902	denotes	transgene
T23605	1192-1194	PR_EXT:000006300	denotes	E2
T23604	1174-1182	SO_EXT:0000167	denotes	promoter
T23603	1170-1173	PR_EXT:000005308	denotes	LAP
T23602	1155-1160	UBERON:0002107	denotes	liver
T23601	1114-1124	GO:0065007	denotes	controlled
T23600	1101-1113	CHEBI:27902	denotes	tetracycline
T23599	1089-1096	GO:0010467	denotes	express
T23598	1084-1088	NCBITaxon:10088	denotes	mice
T23597	1036-1040	NCBITaxon:10088	denotes	mice
T23596	1025-1035	SO_EXT:transgenic_entity	denotes	transgenic
T23595	1017-1020	PR_EXT:000005308	denotes	LAP
T23594	1013-1015	PR_EXT:000006300	denotes	E2
T23593	1007-1012	NCBITaxon:9606	denotes	human
T23592	999-1006	GO:0010467	denotes	express
T23591	989-993	NCBITaxon:10088	denotes	mice
T23590	953-963	SO_EXT:transgenic_entity	denotes	Transgenic
T23589	911-916	NCBITaxon:10088	denotes	mouse
T23588	900-910	SO_EXT:transgenic_entity	denotes	Transgenic
T23654	2132-2139	CHEBI_PR_EXT:protein	denotes	protein
T23653	2104-2112	GO:0042571	denotes	antibody
T23652	2098-2103	PR_EXT:000003676	denotes	actin
T23651	2056-2063	UBERON:0000479	denotes	tissues
T23650	2044-2046	PR_EXT:000006300	denotes	E2
T23649	2026-2035	SO_EXT:0000902	denotes	transgene
T23648	1984-1990	UBERON_EXT:muscle_structure_or_tissue	denotes	muscle
T23647	1968-1974	UBERON:0002113	denotes	kidney
T23646	1957-1962	UBERON:0000955	denotes	brain
T15725	9283-9287	NCBITaxon:10088	denotes	mice
T15724	9276-9282	SO_EXT:sequence_rescue_process	denotes	rescue
T15723	9105-9110	UBERON:0002107	denotes	liver
T15722	9082-9088	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T15721	9050-9059	GO:0010467	denotes	expressed
T15720	8972-8979	CHEBI_SO_EXT:alanine	denotes	alanine
T15718	8958-8962	NCBITaxon:10088	denotes	mice
T15717	8898-8902	GO:0016265	denotes	died
T15716	8840-8844	NCBITaxon:10088	denotes	mice
T15715	8712-8722	GO_EXT:killing	denotes	sacrificed
T15714	8670-8674	NCBITaxon:10088	denotes	mice
T15713	8631-8635	NCBITaxon:10088	denotes	mice
T15712	8624-8630	SO_EXT:sequence_rescue_process	denotes	rescue
T15711	8553-8560	NCBITaxon:33208	denotes	animals
T15710	8449-8459	SO_EXT:transgenic_entity	denotes	transgenic
T15709	8429-8433	NCBITaxon:10088	denotes	mice
T15708	8394-8401	UBERON:0000479	denotes	tissues
T15707	8368-8370	PR_EXT:000006300	denotes	E2
T15706	8354-8364	GO:0010467	denotes	expression
T15705	8300-8306	UBERON_EXT:muscle_structure_or_tissue	denotes	muscle
T15704	8289-8295	UBERON:0002113	denotes	kidney
T15703	8282-8287	UBERON:0000955	denotes	brain
T15702	8269-8278	SO_EXT:0000902	denotes	transgene
T15701	8266-8268	PR_EXT:000006300	denotes	E2
T15700	8248-8258	GO:0010467	denotes	expression
T15699	8184-8194	CHEBI_SO_EXT:amino_acid	denotes	amino acid
T15698	8174-8176	PR_EXT:000006300	denotes	E2
T15697	8168-8173	NCBITaxon:10088	denotes	mouse
T15696	8141-8143	PR_EXT:000006300	denotes	E2
T15673	7523-7525	PR_EXT:000006300	denotes	E2
T15672	7505-7514	SO_EXT:0000902	denotes	transgene
T15671	7476-7482	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T15670	7431-7438	CHEBI_PR_EXT:protein	denotes	protein
T15669	7428-7430	PR_EXT:000006300	denotes	E2
T15668	7371-7375	NCBITaxon:10088	denotes	mice
T15667	7352-7362	SO_EXT:transgenic_entity	denotes	transgenic
T15666	7340-7345	UBERON:0002107	denotes	liver
T15665	7316-7318	PR_EXT:000006300	denotes	E2
T15664	7310-7315	NCBITaxon:10088	denotes	mouse
T15663	7266-7268	PR_EXT:000006300	denotes	E2
T15662	7260-7265	NCBITaxon:9606	denotes	human
T15661	7241-7245	NCBITaxon:10088	denotes	mice
T15660	7230-7240	SO_EXT:transgenic_entity	denotes	transgenic
T15659	7207-7211	NCBITaxon:10088	denotes	mice
T15658	7175-7179	NCBITaxon:10088	denotes	mice
T15657	7131-7133	PR_EXT:000006300	denotes	E2
T15656	7125-7130	NCBITaxon:9606	denotes	human
T15655	7073-7078	UBERON:0002107	denotes	liver
T15654	7035-7044	CHEBI_GO_SO_EXT:enzyme	denotes	enzymatic
T15653	6971-6976	UBERON:0002107	denotes	liver
T15652	6949-6958	CHEBI_GO_SO_EXT:enzyme	denotes	enzymatic
T15651	6903-6908	UBERON:0002107	denotes	liver
T15650	6892-6899	CHEBI_PR_EXT:protein	denotes	protein
T15649	6889-6891	PR_EXT:000006300	denotes	E2
T15648	6883-6888	NCBITaxon:9606	denotes	human
T15647	6892-6899	GO:0006412	denotes	protein
T15646	6869-6882	_FRAGMENT	denotes	production of
T15645	6849-6855	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T15644	6826-6836	GO:0010467	denotes	expression
T15643	6811-6816	UBERON:0002107	denotes	liver
T15642	6774-6776	PR_EXT:000006300	denotes	E2
T15641	6768-6773	NCBITaxon:9606	denotes	human
T15640	6757-6767	SO_EXT:transgenic_entity	denotes	transgenic
T15639	6743-6753	GO:0010467	denotes	expression
T15638	6714-6718	NCBITaxon:10088	denotes	mice
T15637	6706-6709	PR_EXT:000005308	denotes	LAP
T15636	6010-6014	NCBITaxon:10088	denotes	mice
T15635	5987-5996	CHEBI_EXT:glutamate	denotes	glutamate
T15634	5974-5978	NCBITaxon:10088	denotes	mice
T15633	5941-5950	CHEBI_SO_EXT:glutamine	denotes	glutamine
T15632	5926-5935	CHEBI_EXT:glutamate	denotes	glutamate
T15631	5917-5924	CHEBI_SO_EXT:alanine	denotes	alanine
T15630	5901-5906	UBERON:0000178	denotes	Blood
T15629	5831-5835	NCBITaxon:10088	denotes	mice
T15628	5820-5830	SO_EXT:transgenic_entity	denotes	transgenic
T15627	5797-5804	CHEBI_SO_EXT:alanine	denotes	alanine
T15626	5792-5796	CHEBI:22918	denotes	BCAA
T15625	5671-5675	NCBITaxon:10088	denotes	mice
T15599	7164-7174	SO_EXT:transgenic_entity	denotes	transgenic
T15598	3961-3965	NCBITaxon_UBERON_EXT:pup	denotes	pups
T15597	3890-3894	NCBITaxon:10088	denotes	mice
T15596	3883-3889	SO_EXT:sequence_rescue_process	denotes	rescue
T15595	3766-3776	SO_EXT:transgenic_entity	denotes	transgenic
T15594	3747-3755	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T15593	3740-3746	SO_EXT:sequence_rescue_process	denotes	rescue
T15592	3709-3713	NCBITaxon:10088	denotes	mice
T15591	3657-3667	SO_EXT:0000902	denotes	transgenes
T15590	3623-3625	PR_EXT:000006300	denotes	E2
T15589	3596-3604	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T15588	3589-3595	SO_EXT:sequence_rescue_process	denotes	rescue
T15587	3572-3579	NCBITaxon:33208	denotes	animals
T15586	3504-3512	GO_EXT:breeding	denotes	breeding
T15585	3455-3464	GO_EXT:fatality_or_lethality	denotes	lethality
T15584	3446-3454	PATO_UBERON_EXT:neonate_or_newborn	denotes	neonatal
T15583	3421-3427	SO_EXT:sequence_rescue_process	denotes	rescue
T15582	3407-3417	SO_EXT:0000902	denotes	transgenes
T15581	3349-3351	PR_EXT:000006300	denotes	E2
T15580	3330-3339	SO_EXT:0000902	denotes	transgene
T15579	3327-3329	PR_EXT:000006300	denotes	E2
T15578	3308-3317	SO_EXT:0000902	denotes	transgene
T15577	3300-3303	PR_EXT:000005308	denotes	LAP
T15576	3269-3273	NCBITaxon:10088	denotes	mice
T15575	3231-3239	GO_EXT:breeding	denotes	breeding
T15574	3206-3208	PR_EXT:000006300	denotes	E2
T15573	3166-3175	SO_EXT:0000902	denotes	transgene
T15572	3158-3161	PR_EXT:000005308	denotes	LAP
T15571	3126-3130	NCBITaxon:10088	denotes	mice
T15570	3056-3060	NCBITaxon:10088	denotes	mice
T15569	3053-3055	PR_EXT:000006300	denotes	E2
T15568	3038-3048	SO_EXT:transgenic_entity	denotes	Transgenic
T15567	3016-3019	CHEBI_SO_EXT:DNA	denotes	DNA
T15566	3011-3015	UBERON:0002415	denotes	tail
T15540	2184-2188	NCBITaxon:10088	denotes	mice
T15539	2176-2179	PR_EXT:000005308	denotes	LAP
T15538	856-864	SO_EXT:0000167	denotes	promoter
T15537	821-825	SO_EXT:cDNA	denotes	cDNA
T15536	818-820	PR_EXT:000006300	denotes	E2
T15535	812-817	NCBITaxon:9606	denotes	human
T15534	802-809	GO:0010467	denotes	express
T15533	776-785	SO_EXT:0000902	denotes	transgene
T15532	773-775	PR_EXT:000006300	denotes	E2
T15531	741-749	GO_EXT:reaction_or_response	denotes	response
T15530	702-711	SO_EXT:0000167	denotes	promoters
T15529	688-698	GO:0010467	denotes	expression
T15528	651-671	GO_EXT:transcription_factor	denotes	transcription factor
T15527	651-664	GO_EXT:transcription	denotes	transcription
T15526	616-626	GO:0065007	denotes	controlled
T15525	603-615	CHEBI:27902	denotes	tetracycline
T15524	585-595	GO:0010467	denotes	expression
T15523	570-575	UBERON:0002107	denotes	liver
T15522	547-554	GO_EXT:biological_direction_or_guidance	denotes	directs
T15521	532-541	SO_EXT:0000902	denotes	transgene
T15520	524-527	PR_EXT:000005308	denotes	LAP
T15519	499-508	SO_EXT:0000902	denotes	transgene
T15518	496-498	PR_EXT:000006300	denotes	E2
T15517	476-485	SO_EXT:0000902	denotes	transgene
T15516	468-471	PR_EXT:000005308	denotes	LAP
T15515	435-445	SO_EXT:transgenic_entity	denotes	transgenic
T15514	403-405	PR_EXT:000006300	denotes	E2
T15513	390-395	UBERON:0002107	denotes	liver
T15695	8135-8140	NCBITaxon:9606	denotes	Human
T15694	8122-8133	PR_EXT:000006506	denotes	E3 subunits
T15693	8109-8114	NCBITaxon:10088	denotes	mouse
T15692	8094-8103	GO:0032991	denotes	complexed
T15691	8061-8063	PR_EXT:000006300	denotes	E2
T15690	8055-8060	NCBITaxon:9606	denotes	human
T15689	8007-8023	GO:0065003	denotes	subunit assembly
T15688	7968-7975	NCBITaxon:562	denotes	E. coli
T15687	7935-7951	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	carboxy terminus
T15686	7924-7927	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	tag
T15685	7903-7912	SO_EXT:sequence_insertion_entity_or_process	denotes	insertion
T15684	7825-7855	GO:0045254	denotes	pyruvate dehydrogenase complex
T15683	7825-7847	GO_EXT:0004738	denotes	pyruvate dehydrogenase
T15682	7825-7833	CHEBI:15361	denotes	pyruvate
T15681	7777-7793	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	carboxy terminus
T15680	7672-7681	CHEBI_GO_SO_EXT:enzyme	denotes	enzymatic
T15679	7653-7655	PR_EXT:000006300	denotes	E2
T15678	7647-7652	NCBITaxon:9606	denotes	human
T15677	7629-7638	SO_EXT:0000902	denotes	transgene
T15676	7605-7621	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	carboxy terminus
T15675	7594-7597	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	tag
T15674	7588-7593	PR_EXT:000000084	denotes	c-myc
T15719	8967-8971	CHEBI:22918	denotes	BCAA
T15512	384-386	PR_EXT:000006300	denotes	E2
T15511	378-383	NCBITaxon:9606	denotes	human
T15510	370-377	GO:0010467	denotes	express
T15509	349-359	SO_EXT:transgenic_entity	denotes	transgenic
T15508	296-301	NCBITaxon:10088	denotes	mouse
T15507	254-263	GO_EXT:fatality_or_lethality	denotes	lethality
T15506	245-253	PATO_UBERON_EXT:neonate_or_newborn	denotes	neonatal
T15505	236-240	CHEBI:22918	denotes	BCAA
T15504	205-211	SO_EXT:sequence_rescue_process	denotes	rescue
T15503	182-192	SO_EXT:transgenic_entity	denotes	transgenic
T15502	117-123	NCBITaxon:39107	denotes	murine
T15501	36-45	SO_EXT:0000902	denotes	transgene
T15500	33-35	PR_EXT:000006300	denotes	E2
T15499	27-32	NCBITaxon:9606	denotes	human
T15498	14-24	GO:0010467	denotes	expressing
T15497	9-13	NCBITaxon:10088	denotes	mice
T15565	2958-2962	SO_EXT:sequence_copy_entity	denotes	copy
T15564	2948-2957	SO_EXT:0000902	denotes	transgene
T15563	2797-2807	SO_EXT:transgenic_entity	denotes	transgenic
T15562	2725-2740	SO_EXT:0000366	denotes	insertion sites
T15561	2715-2724	SO_EXT:0000902	denotes	transgene
T15560	2694-2701	NCBITaxon:33208	denotes	animals
T15559	2641-2649	GO_EXT:breeding	denotes	breeding
T15558	2601-2610	SO_EXT:0000902	denotes	transgene
T15557	2498-2508	SO_EXT:transgenic_entity	denotes	transgenic
T15556	2412-2421	SO_EXT:0000902	denotes	transgene
T15555	2350-2359	SO_EXT:0000902	denotes	transgene
T15554	2347-2349	PR_EXT:000006300	denotes	E2
T15553	2320-2324	NCBITaxon:10088	denotes	mice
T15552	2309-2319	SO_EXT:transgenic_entity	denotes	transgenic
T15551	2263-2273	GO:0045120	denotes	Pronuclear
T15550	2227-2228	NCBITaxon:10088	denotes	i
T15549	2221-2222	CHEBI_PR_EXT:protein	denotes	r
T15548	2218-2219	UBERON_EXT:muscle_structure_or_tissue	denotes	c
T15547	2212-2213	UBERON:0002113	denotes	d
T15546	2211-2212	NCBITaxon:10088	denotes	e
T15545	2210-2211	SO_EXT:transgenic_entity	denotes	c
T15544	2209-2210	NCBITaxon:9606	denotes	u
T15543	2208-2209	GO:0042571	denotes	d
T15542	2197-2198	UBERON:0002107	denotes	v
T15541	2194-2197	CHEBI_PR_EXT:protein	denotes	pre
T15624	5652-5659	CHEBI_SO_EXT:alanine	denotes	alanine
T15623	5647-5651	CHEBI:22918	denotes	BCAA
T15622	5634-5641	CHEBI_SO_EXT:alanine	denotes	alanine
T15621	5626-5630	CHEBI:22918	denotes	BCAA
T15620	5576-5586	CHEBI_SO_EXT:amino_acid	denotes	amino acid
T15619	5570-5575	UBERON:0000178	denotes	blood
T15618	5523-5524	GO_EXT:killing	denotes	c
T15617	5489-5490	UBERON:0000178	denotes	d
T15616	5487-5489	CHEBI_SO_EXT:alanine	denotes	an
T15615	5485-5486	CHEBI:22918	denotes	A
T15614	5482-5483	SO_EXT:genotype_or_entity_with_genotype	denotes	e
T15613	5479-5480	PR_EXT:000005308	denotes	L
T15612	4305-4309	NCBITaxon_UBERON_EXT:pup	denotes	pups
T15611	4255-4262	NCBITaxon:33208	denotes	animals
T15610	4248-4254	SO_EXT:sequence_rescue_process	denotes	rescue
T15609	4188-4194	GO_EXT:fatality_or_lethality	denotes	lethal
T15608	4179-4187	PATO_UBERON_EXT:neonate_or_newborn	denotes	neonatal
T15607	4168-4174	SO_EXT:sequence_rescue_process	denotes	rescue
T15606	4111-4113	PR_EXT:000006300	denotes	E2
T15605	4087-4093	GO_EXT:fatality_or_lethality	denotes	lethal
T15604	4078-4086	PATO_UBERON_EXT:neonate_or_newborn	denotes	neonatal
T15603	4065-4073	SO_EXT:sequence_rescue_process	denotes	rescuing
T15602	4021-4030	SO_EXT:0000902	denotes	transgene
T15601	3996-4004	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T15600	3989-3995	SO_EXT:sequence_rescue_process	denotes	rescue
R3834	T15647	T15646	_lexicallyChainedTo	protein,production of

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T24910	6229-6233	NCBITaxon:10088	denotes	mice
T24909	6079-6084	UBERON:0000178	denotes	blood
T24414	5354-5358	NCBITaxon:10088	denotes	mice
T24413	5319-5323	NCBITaxon:10088	denotes	mice
T24412	5260-5264	NCBITaxon:10088	denotes	mice
T24411	5222-5227	UBERON:0002107	denotes	liver
T24410	4990-4994	CHEBI:22918	denotes	BCAA
T24409	4931-4935	CHEBI:22918	denotes	BCAA
T24408	4884-4888	NCBITaxon:10088	denotes	mice
T24407	4875-4880	UBERON:0000178	denotes	blood
T24406	4856-4860	CHEBI:22918	denotes	BCAA
T24405	4752-4759	NCBITaxon:33208	denotes	animals
T24404	4632-4642	SO:0000902	denotes	transgenes
T24403	4629-4631	PR:000006300	denotes	E2
T24402	4613-4616	PR:000005308	denotes	LAP
T24401	4584-4586	PR:000006300	denotes	E2
T24400	4455-4459	NCBITaxon:10088	denotes	mice
T24399	4363-4369	NCBITaxon:10088	denotes	murine
T23587	2104-2112	GO:0042571	denotes	antibody
T23586	2098-2103	PR:000003676	denotes	actin
T23585	2056-2063	UBERON:0000479	denotes	tissues
T23584	2044-2046	PR:000006300	denotes	E2
T23583	2026-2035	SO:0000902	denotes	transgene
T23582	1968-1974	UBERON:0002113	denotes	kidney
T23581	1957-1962	UBERON:0000955	denotes	brain
T23580	1906-1910	NCBITaxon:10088	denotes	mice
T23579	1889-1891	PR:000006300	denotes	E2
T23578	1883-1888	NCBITaxon:9606	denotes	human
T23577	1869-1874	PR:000000084	denotes	c-myc
T23576	1850-1859	SO:0000902	denotes	transgene
T23575	1811-1819	GO:0042571	denotes	antibody
T23574	1801-1806	PR:000000084	denotes	c-myc
T23573	1774-1781	NCBITaxon:33208	denotes	animals
T23572	1748-1750	PR:000006300	denotes	E2
T15496	9283-9287	NCBITaxon:10088	denotes	mice
T15495	9105-9110	UBERON:0002107	denotes	liver
T15494	9050-9059	GO:0010467	denotes	expressed
T15493	8967-8971	CHEBI:22918	denotes	BCAA
T15492	8958-8962	NCBITaxon:10088	denotes	mice
T15491	8898-8902	GO:0016265	denotes	died
T15490	8840-8844	NCBITaxon:10088	denotes	mice
T15489	8670-8674	NCBITaxon:10088	denotes	mice
T15488	8631-8635	NCBITaxon:10088	denotes	mice
T15487	8553-8560	NCBITaxon:33208	denotes	animals
T15486	8429-8433	NCBITaxon:10088	denotes	mice
T15485	8394-8401	UBERON:0000479	denotes	tissues
T15484	8368-8370	PR:000006300	denotes	E2
T15483	8354-8364	GO:0010467	denotes	expression
T15473	8122-8133	PR:000006506	denotes	E3 subunits
T15472	8109-8114	NCBITaxon:10088	denotes	mouse
T15471	8094-8103	GO:0032991	denotes	complexed
T15470	8061-8063	PR:000006300	denotes	E2
T15469	8055-8060	NCBITaxon:9606	denotes	human
T15468	8007-8023	GO:0065003	denotes	subunit assembly
T15467	7968-7975	NCBITaxon:562	denotes	E. coli
T15466	7825-7855	GO:0045254	denotes	pyruvate dehydrogenase complex
T15465	7825-7833	CHEBI:15361	denotes	pyruvate
T15464	7653-7655	PR:000006300	denotes	E2
T15463	7647-7652	NCBITaxon:9606	denotes	human
T15462	7629-7638	SO:0000902	denotes	transgene
T15461	7588-7593	PR:000000084	denotes	c-myc
T15460	7523-7525	PR:000006300	denotes	E2
T15459	7505-7514	SO:0000902	denotes	transgene
T15458	7428-7430	PR:000006300	denotes	E2
T15457	7371-7375	NCBITaxon:10088	denotes	mice
T15456	7340-7345	UBERON:0002107	denotes	liver
T15455	7316-7318	PR:000006300	denotes	E2
T15454	7310-7315	NCBITaxon:10088	denotes	mouse
T15453	7266-7268	PR:000006300	denotes	E2
T15452	7260-7265	NCBITaxon:9606	denotes	human
T15451	7241-7245	NCBITaxon:10088	denotes	mice
T15450	7207-7211	NCBITaxon:10088	denotes	mice
T15449	7175-7179	NCBITaxon:10088	denotes	mice
T15448	7131-7133	PR:000006300	denotes	E2
T15447	7125-7130	NCBITaxon:9606	denotes	human
T15446	7073-7078	UBERON:0002107	denotes	liver
T15445	6971-6976	UBERON:0002107	denotes	liver
T15444	6903-6908	UBERON:0002107	denotes	liver
T15443	6889-6891	PR:000006300	denotes	E2
T15442	6883-6888	NCBITaxon:9606	denotes	human
T15441	6892-6899	GO:0006412	denotes	protein
T15440	6869-6882	_FRAGMENT	denotes	production of
T15439	6826-6836	GO:0010467	denotes	expression
T15438	6811-6816	UBERON:0002107	denotes	liver
T15437	6774-6776	PR:000006300	denotes	E2
T15436	6768-6773	NCBITaxon:9606	denotes	human
T15435	6743-6753	GO:0010467	denotes	expression
T15434	6714-6718	NCBITaxon:10088	denotes	mice
T15433	6706-6709	PR:000005308	denotes	LAP
T15432	6010-6014	NCBITaxon:10088	denotes	mice
T15380	856-864	SO:0000167	denotes	promoter
T15379	818-820	PR:000006300	denotes	E2
T15378	812-817	NCBITaxon:9606	denotes	human
T15377	802-809	GO:0010467	denotes	express
T15376	776-785	SO:0000902	denotes	transgene
T15375	773-775	PR:000006300	denotes	E2
T15374	702-711	SO:0000167	denotes	promoters
T15373	688-698	GO:0010467	denotes	expression
T15372	616-626	GO:0065007	denotes	controlled
T15371	603-615	CHEBI:27902	denotes	tetracycline
T15370	585-595	GO:0010467	denotes	expression
T15369	570-575	UBERON:0002107	denotes	liver
T15368	532-541	SO:0000902	denotes	transgene
T15367	524-527	PR:000005308	denotes	LAP
T15366	499-508	SO:0000902	denotes	transgene
T15365	496-498	PR:000006300	denotes	E2
T15364	476-485	SO:0000902	denotes	transgene
T15363	468-471	PR:000005308	denotes	LAP
T15362	403-405	PR:000006300	denotes	E2
T15361	390-395	UBERON:0002107	denotes	liver
T15360	384-386	PR:000006300	denotes	E2
T15359	378-383	NCBITaxon:9606	denotes	human
T15358	370-377	GO:0010467	denotes	express
T15357	296-301	NCBITaxon:10088	denotes	mouse
T15356	236-240	CHEBI:22918	denotes	BCAA
T15355	117-123	NCBITaxon:39107	denotes	murine
T15354	36-45	SO:0000902	denotes	transgene
T15353	33-35	PR:000006300	denotes	E2
T15352	27-32	NCBITaxon:9606	denotes	human
T15351	14-24	GO:0010467	denotes	expressing
T15350	9-13	NCBITaxon:10088	denotes	mice
T15482	8289-8295	UBERON:0002113	denotes	kidney
T15481	8282-8287	UBERON:0000955	denotes	brain
T15480	8269-8278	SO:0000902	denotes	transgene
T15479	8266-8268	PR:000006300	denotes	E2
T15478	8248-8258	GO:0010467	denotes	expression
T15477	8174-8176	PR:000006300	denotes	E2
T15476	8168-8173	NCBITaxon:10088	denotes	mouse
T15475	8141-8143	PR:000006300	denotes	E2
T15474	8135-8140	NCBITaxon:9606	denotes	Human
T23571	1742-1747	NCBITaxon:10088	denotes	mouse
T23570	1694-1701	NCBITaxon:33208	denotes	animals
T23569	1634-1638	NCBITaxon:10088	denotes	mice
T23568	1598-1600	PR:000006300	denotes	E2
T23567	1592-1597	NCBITaxon:9606	denotes	human
T23566	1562-1566	NCBITaxon:10088	denotes	mice
T23565	1523-1528	UBERON:0002107	denotes	liver
T23564	1509-1511	PR:000006300	denotes	E2
T23563	1475-1479	NCBITaxon:10088	denotes	mice
T23562	1387-1411	SO:0000610	denotes	polyadenylation sequence
T23561	1387-1402	GO:0043631	denotes	polyadenylation
T23560	1374-1378	NCBITaxon:10633	denotes	SV40
T23559	1351-1356	PR:000000084	denotes	c-myc
T23558	1321-1323	PR:000006300	denotes	E2
T23557	1315-1320	NCBITaxon:9606	denotes	human
T23556	1291-1297	SO:0000188	denotes	intron
T23555	1269-1277	SO:0000167	denotes	promoter
T23554	1218-1230	CHEBI:27902	denotes	tetracycline
T23553	1195-1204	SO:0000902	denotes	transgene
T23552	1192-1194	PR:000006300	denotes	E2
T23551	1174-1182	SO:0000167	denotes	promoter
T23550	1170-1173	PR:000005308	denotes	LAP
T23549	1155-1160	UBERON:0002107	denotes	liver
T23548	1114-1124	GO:0065007	denotes	controlled
T23547	1101-1113	CHEBI:27902	denotes	tetracycline
T23546	1089-1096	GO:0010467	denotes	express
T23545	1084-1088	NCBITaxon:10088	denotes	mice
T23544	1036-1040	NCBITaxon:10088	denotes	mice
T23543	1017-1020	PR:000005308	denotes	LAP
T23542	1013-1015	PR:000006300	denotes	E2
T23541	1007-1012	NCBITaxon:9606	denotes	human
T23540	999-1006	GO:0010467	denotes	express
T23539	989-993	NCBITaxon:10088	denotes	mice
T23538	911-916	NCBITaxon:10088	denotes	mouse
T15423	5489-5490	UBERON:0000178	denotes	d
T15422	5485-5486	CHEBI:22918	denotes	A
T15421	5479-5480	PR:000005308	denotes	L
T15420	4255-4262	NCBITaxon:33208	denotes	animals
T15419	4111-4113	PR:000006300	denotes	E2
T15418	4021-4030	SO:0000902	denotes	transgene
T15417	3890-3894	NCBITaxon:10088	denotes	mice
T15416	3709-3713	NCBITaxon:10088	denotes	mice
T15415	3657-3667	SO:0000902	denotes	transgenes
T15414	3623-3625	PR:000006300	denotes	E2
T15413	3572-3579	NCBITaxon:33208	denotes	animals
T15412	3407-3417	SO:0000902	denotes	transgenes
T15411	3349-3351	PR:000006300	denotes	E2
T15410	3330-3339	SO:0000902	denotes	transgene
T15409	3327-3329	PR:000006300	denotes	E2
T15408	3308-3317	SO:0000902	denotes	transgene
T15407	3300-3303	PR:000005308	denotes	LAP
T15406	3269-3273	NCBITaxon:10088	denotes	mice
T15405	3206-3208	PR:000006300	denotes	E2
T15404	3166-3175	SO:0000902	denotes	transgene
T15403	3158-3161	PR:000005308	denotes	LAP
T15402	3126-3130	NCBITaxon:10088	denotes	mice
T15401	3056-3060	NCBITaxon:10088	denotes	mice
T15400	3053-3055	PR:000006300	denotes	E2
T15399	3011-3015	UBERON:0002415	denotes	tail
T15398	2948-2957	SO:0000902	denotes	transgene
T15397	2725-2740	SO:0000366	denotes	insertion sites
T15396	2715-2724	SO:0000902	denotes	transgene
T15395	2694-2701	NCBITaxon:33208	denotes	animals
T15394	2601-2610	SO:0000902	denotes	transgene
T15393	2412-2421	SO:0000902	denotes	transgene
T15392	2350-2359	SO:0000902	denotes	transgene
T15391	2347-2349	PR:000006300	denotes	E2
T15390	2320-2324	NCBITaxon:10088	denotes	mice
T15389	2263-2273	GO:0045120	denotes	Pronuclear
T15388	2227-2228	NCBITaxon:10088	denotes	i
T15387	2212-2213	UBERON:0002113	denotes	d
T15386	2211-2212	NCBITaxon:10088	denotes	e
T15385	2209-2210	NCBITaxon:9606	denotes	u
T15384	2208-2209	GO:0042571	denotes	d
T15383	2197-2198	UBERON:0002107	denotes	v
T15382	2184-2188	NCBITaxon:10088	denotes	mice
T15381	2176-2179	PR:000005308	denotes	LAP
T15431	5974-5978	NCBITaxon:10088	denotes	mice
T15430	5901-5906	UBERON:0000178	denotes	Blood
T15429	5831-5835	NCBITaxon:10088	denotes	mice
T15428	5792-5796	CHEBI:22918	denotes	BCAA
T15427	5671-5675	NCBITaxon:10088	denotes	mice
T15426	5647-5651	CHEBI:22918	denotes	BCAA
T15425	5626-5630	CHEBI:22918	denotes	BCAA
T15424	5570-5575	UBERON:0000178	denotes	blood
R3833	T15441	T15440	_lexicallyChainedTo	protein,production of

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