PMC:1448208 / 16335-32301 JSON TXT

Annnotations TAB JSON ListView MergeView

craft-sa-dev

Id	Subject	Object	Predicate	Lexical cue
T24960	12902-12903	.	denotes	.
T24959	12899-12902	NN	denotes	SEM
T24958	12897-12898	SYM	denotes	-
T24957	12896-12897	HYPH	denotes	/
T24956	12895-12896	SYM	denotes	+
T24955	12890-12894	NN	denotes	mean
T24954	12886-12889	VBP	denotes	are
T24953	12879-12885	NNS	denotes	values
T24952	12875-12878	DT	denotes	All
T24951	12874-12903	sentence	denotes	All values are mean +/- SEM.
T24950	12873-12874	.	denotes	.
T24949	12870-12873	NN	denotes	age
T24948	12867-12869	IN	denotes	of
T24947	12861-12866	NNS	denotes	weeks
T24946	12858-12859	SYM	denotes	–
T24945	12859-12860	CD	denotes	6
T24944	12857-12858	CD	denotes	4
T24943	12852-12856	VBD	denotes	were
T24942	12847-12851	WDT	denotes	that
T24941	12842-12846	NNS	denotes	mice
T24497	11226-11229	NN	denotes	LAP
T24496	11222-11225	DT	denotes	the
T24495	11245-11255	NNS	denotes	transgenes
T24494	11217-11221	CC	denotes	both
T24493	11213-11216	IN	denotes	for
T24492	11204-11212	JJ	denotes	positive
T24491	11200-11203	CC	denotes	and
T24490	11197-11199	NN	denotes	E2
T24489	11186-11196	JJ	denotes	endogenous
T24482	11152-11153	-LRB-	denotes	(
T24481	11136-11142	NN	denotes	rescue
T24480	11143-11151	NN	denotes	genotype
T24479	11132-11135	DT	denotes	the
T24478	11128-11131	VBD	denotes	had
T24477	11123-11127	WDT	denotes	that
T24476	11116-11122	VBN	denotes	tested
T24475	11100-11110	JJ	denotes	transgenic
T24474	11111-11115	NN	denotes	line
T24473	11095-11099	DT	denotes	each
T24472	11090-11094	IN	denotes	from
T24471	11082-11089	VBG	denotes	weaning
T24470	11079-11081	IN	denotes	at
T24469	11073-11078	JJ	denotes	alive
T24468	11068-11072	NNS	denotes	mice
T24467	11065-11067	IN	denotes	of
T24466	11053-11064	NNS	denotes	percentages
T24465	11049-11052	VBP	denotes	are
T24464	11039-11048	VBN	denotes	Presented
T24463	11038-11257	sentence	denotes	Presented are percentages of mice alive at weaning from each transgenic line tested that had the rescue genotype (i.e., homozygous for knockout of endogenous E2 and positive for both the LAP-tTA and TRE-E2 transgenes).
T24462	11037-11038	.	denotes	.
T24461	11025-11031	NN	denotes	rescue
T24460	11032-11037	NNS	denotes	lines
T24459	11014-11024	JJ	denotes	transgenic
T24458	11011-11013	IN	denotes	of
T24457	10993-11001	NN	denotes	Survival
T24456	10991-10993	,	denotes	,
T24455	11002-11010	NN	denotes	analysis
T24454	10990-10991	LS	denotes	A
T24453	10989-11038	sentence	denotes	A, Survival analysis of transgenic rescue lines.
T24452	10988-10989	.	denotes	.
T24451	10976-10982	JJ	denotes	murine
T24450	10971-10975	NN	denotes	MSUD
T24449	10958-10970	JJ	denotes	intermediate
T24448	10983-10988	NN	denotes	model
T24447	10954-10957	DT	denotes	the
T24446	10951-10953	IN	denotes	of
T24445	10934-10950	NN	denotes	Characterization
T24940	12837-12841	IN	denotes	from
T24939	12822-12826	VBD	denotes	were
T24938	12827-12836	VBN	denotes	collected
T24937	12814-12821	NNS	denotes	Samples
T24936	12813-12874	sentence	denotes	Samples were collected from mice that were 4–6 weeks of age.
T24935	12812-12813	.	denotes	.
T24934	12795-12799	NN	denotes	mass
T24933	12800-12812	NN	denotes	spectrometry
T24932	12788-12794	JJ	denotes	tandem
T24931	12785-12787	IN	denotes	by
T24930	12774-12784	VBN	denotes	determined
T24929	12771-12773	IN	denotes	as
T24928	12762-12770	NNS	denotes	controls
T24927	12751-12761	NN	denotes	littermate
T24926	12747-12750	CC	denotes	and
T24925	12736-12740	NN	denotes	MSUD
T24924	12723-12735	JJ	denotes	intermediate
T24923	12741-12746	NN	denotes	model
T24922	12719-12722	DT	denotes	the
T24921	12716-12718	IN	denotes	in
T24920	12704-12708	NN	denotes	acid
T24919	12698-12703	NN	denotes	amino
T24918	12692-12697	NN	denotes	blood
T24917	12709-12715	NNS	denotes	levels
T24916	12681-12691	JJ	denotes	additional
T24915	12678-12680	IN	denotes	of
T24914	12670-12677	NN	denotes	Summary
T24680	12046-12056	VBN	denotes	sacrificed
T24679	12033-12045	RB	denotes	subsequently
T24678	12029-12032	CC	denotes	and
T24677	12020-12028	JJ	denotes	moribund
T24676	12010-12014	PRP	denotes	they
T24675	12015-12019	VBD	denotes	were
T24674	12004-12009	IN	denotes	until
T24673	11989-11993	VBD	denotes	were
T24672	11981-11988	NN	denotes	weaning
T24671	11978-11980	IN	denotes	at
T24670	11972-11977	JJ	denotes	alive
T24669	11994-12003	VBN	denotes	monitored
T24668	11967-11971	NNS	denotes	mice
T24667	11960-11966	NN	denotes	Rescue
T24666	11959-12091	sentence	denotes	Rescue mice alive at weaning were monitored until they were moribund and subsequently sacrificed or were found dead in their cages.
T24665	11958-11959	.	denotes	.
T24664	11954-11958	NN	denotes	525A
T24663	11950-11953	CC	denotes	and
T24662	11948-11949	NN	denotes	A
T24661	11942-11947	NNS	denotes	Lines
T24660	11937-11941	IN	denotes	from
T24659	11932-11936	NNS	denotes	mice
T24658	11928-11931	IN	denotes	for
T24657	11912-11920	NN	denotes	Survival
T24656	11910-11912	,	denotes	,
T24655	11921-11927	NNS	denotes	curves
T24654	11909-11910	LS	denotes	D
T24653	11908-11959	sentence	denotes	D, Survival curves for mice from Lines A and 525A.
T24652	11907-11908	.	denotes	.
T24651	11899-11900	SYM	denotes	≤
T24650	11897-11898	NN	denotes	P
T24649	11895-11897	,	denotes	,
T24648	11893-11895	SYM	denotes	**
T24647	11891-11892	:	denotes	;
T24646	11901-11907	CD	denotes	0.0001
T24645	11884-11885	SYM	denotes	≤
T24644	11882-11883	NN	denotes	P
T24643	11880-11882	,	denotes	,
T24642	11886-11891	CD	denotes	0.001
T24641	11879-11880	SYM	denotes	*
T24640	11878-11908	sentence	denotes	, P ≤ 0.001; *, P ≤ 0.0001.
T24639	11877-11878	.	denotes	.
T24638	11868-11872	NN	denotes	525A
T24637	11864-11867	CC	denotes	and
T24636	11862-11863	NN	denotes	A
T24635	11856-11861	NNS	denotes	Lines
T24634	11852-11855	CC	denotes	and
T24633	11873-11877	NNS	denotes	mice
T24632	11844-11851	NN	denotes	control
T24631	11841-11843	IN	denotes	of
T24630	11835-11840	NN	denotes	liver
T24629	11832-11834	IN	denotes	in
T24628	11816-11822	NN	denotes	enzyme
T24627	11810-11815	NN	denotes	BCKDH
T24626	11808-11810	,	denotes	,
T24625	11823-11831	NN	denotes	activity
T24624	11807-11808	LS	denotes	C
T24623	11806-11878	sentence	denotes	C, BCKDH enzyme activity in liver of control and Lines A and 525A mice.
T24622	11805-11806	.	denotes	.
T24621	11798-11799	SYM	denotes	<
T24620	11796-11797	NN	denotes	P
T24619	11794-11796	,	denotes	,
T24618	11792-11794	SYM	denotes	**
T24617	11790-11791	:	denotes	;
T24616	11800-11805	CD	denotes	0.005
T24615	11784-11785	SYM	denotes	≤
T24614	11782-11783	NN	denotes	P
T24613	11780-11782	,	denotes	,
T24612	11786-11790	CD	denotes	0.01
T24611	11779-11780	SYM	denotes	*
T24610	11778-11806	sentence	denotes	, P ≤ 0.01; *, P < 0.005.
T24609	11777-11778	.	denotes	.
T24608	11769-11777	VBN	denotes	analyzed
T24607	11761-11768	NNS	denotes	samples
T24606	11758-11760	IN	denotes	of
T24605	11750-11757	NNS	denotes	numbers
T24604	11746-11749	DT	denotes	the
T24603	11731-11735	NNS	denotes	bars
T24602	11727-11730	DT	denotes	the
T24601	11724-11726	IN	denotes	on
T24600	11736-11745	VBZ	denotes	indicates
T24599	11716-11723	NNS	denotes	numbers
T24598	11712-11715	DT	denotes	The
T24597	11711-11778	sentence	denotes	The numbers on the bars indicates the numbers of samples analyzed.
T24596	11710-11711	.	denotes	.
T24595	11704-11710	VBN	denotes	tested
T24594	11699-11703	NNS	denotes	ages
T24593	11695-11698	DT	denotes	all
T24592	11692-11694	IN	denotes	at
T24591	11683-11691	NNS	denotes	controls
T24590	11680-11682	IN	denotes	to
T24589	11671-11679	VBN	denotes	compared
T24588	11666-11670	NN	denotes	525A
T24587	11662-11665	CC	denotes	and
T24586	11660-11661	NN	denotes	A
T24585	11654-11659	NNS	denotes	Lines
T24584	11650-11653	IN	denotes	for
T24583	11642-11649	JJR	denotes	greater
T24582	11628-11641	RB	denotes	significantly
T24581	11623-11627	VBD	denotes	were
T24580	11616-11622	NNS	denotes	values
T24579	11607-11608	HYPH	denotes	/
T24578	11608-11615	NN	denotes	alanine
T24577	11603-11607	NN	denotes	BCAA
T24576	11602-11711	sentence	denotes	BCAA/alanine values were significantly greater for Lines A and 525A compared to controls at all ages tested.
T24575	11601-11602	.	denotes	.
T24574	11595-11601	NN	denotes	valine
T24573	11591-11594	CC	denotes	and
T24572	11589-11591	,	denotes	,
T24571	11579-11589	NN	denotes	isoleucine
T24570	11577-11579	,	denotes	,
T24569	11570-11577	NN	denotes	leucine
T24568	11567-11569	IN	denotes	of
T24567	11563-11566	NN	denotes	sum
T24566	11559-11562	DT	denotes	the
T24565	11549-11558	VBP	denotes	represent
T24564	11544-11548	NN	denotes	BCAA
T24563	11538-11543	JJ	denotes	Total
T24562	11537-11602	sentence	denotes	Total BCAA represent the sum of leucine, isoleucine, and valine.
T24561	11536-11537	.	denotes	.
T24560	11532-11536	NN	denotes	525A
T24559	11528-11531	CC	denotes	and
T24558	11526-11527	NN	denotes	A
T24557	11520-11525	NNS	denotes	Lines
T24556	11516-11519	CC	denotes	and
T24555	11507-11515	NNS	denotes	controls
T24554	11502-11506	IN	denotes	from
T24553	11497-11501	NNS	denotes	mice
T24552	11494-11496	IN	denotes	of
T24551	11488-11493	NN	denotes	blood
T24550	11485-11487	IN	denotes	in
T24549	11477-11484	NN	denotes	alanine
T24548	11474-11476	IN	denotes	to
T24547	11469-11473	NN	denotes	BCAA
T24546	11463-11468	JJ	denotes	total
T24545	11460-11462	IN	denotes	of
T24544	11452-11454	,	denotes	,
T24543	11454-11459	NN	denotes	Ratio
T24542	11451-11452	LS	denotes	B
T24541	11450-11537	sentence	denotes	B, Ratio of total BCAA to alanine in blood of mice from controls and Lines A and 525A.
T24540	11449-11450	.	denotes	.
T24539	11445-11449	NN	denotes	line
T24538	11440-11444	DT	denotes	each
T24537	11436-11439	IN	denotes	for
T24536	11423-11435	NNS	denotes	observations
T24535	11420-11422	IN	denotes	of
T24534	11412-11419	NNS	denotes	numbers
T24533	11408-11411	DT	denotes	the
T24532	11393-11397	NNS	denotes	bars
T24531	11389-11392	DT	denotes	the
T24530	11386-11388	IN	denotes	on
T24529	11398-11407	VBZ	denotes	indicates
T24528	11378-11385	NNS	denotes	numbers
T24527	11374-11377	DT	denotes	The
T24526	11373-11450	sentence	denotes	The numbers on the bars indicates the numbers of observations for each line.
T24525	11372-11373	.	denotes	.
T24524	11365-11372	NNS	denotes	animals
T24523	11362-11364	IN	denotes	of
T24522	11351-11361	NN	denotes	population
T24521	11346-11350	DT	denotes	this
T24520	11343-11345	IN	denotes	in
T24519	11331-11333	VBZ	denotes	is
T24518	11322-11330	NN	denotes	genotype
T24517	11317-11321	DT	denotes	this
T24516	11311-11316	WDT	denotes	which
T24515	11334-11342	VBN	denotes	expected
T24514	11308-11310	IN	denotes	at
T24513	11290-11297	JJ	denotes	maximum
T24512	11278-11289	JJ	denotes	theoretical
T24511	11298-11307	NN	denotes	frequency
T24510	11274-11277	DT	denotes	the
T24509	11263-11270	VBN	denotes	plotted
T24508	11271-11273	VBZ	denotes	is
T24507	11258-11262	RB	denotes	Also
T24506	11257-11373	sentence	denotes	Also plotted is the theoretical maximum frequency at which this genotype is expected in this population of animals.
T24505	11256-11257	.	denotes	.
T24504	11255-11256	-RRB-	denotes	)
T24503	11241-11242	HYPH	denotes	-
T24502	11242-11244	NN	denotes	E2
T24501	11238-11241	NN	denotes	TRE
T24500	11234-11237	CC	denotes	and
T24499	11229-11230	HYPH	denotes	-
T24498	11230-11233	NN	denotes	tTA
T24688	12090-12091	.	denotes	.
T23927	8776-8778	VB	denotes	be
T23926	8773-8775	TO	denotes	to
T23925	8768-8772	NN	denotes	lane
T23924	8763-8767	DT	denotes	each
T23923	8760-8762	IN	denotes	in
T23922	8753-8759	VBN	denotes	loaded
T23921	8745-8752	NN	denotes	protein
T23920	8742-8744	IN	denotes	of
T23919	8779-8787	VBN	denotes	compared
T23918	8735-8741	NN	denotes	amount
T23917	8729-8734	VB	denotes	allow
T23916	8726-8728	TO	denotes	to
T23915	8711-8716	NN	denotes	actin
T23914	8717-8725	NN	denotes	antibody
T23913	8708-8710	DT	denotes	an
T23912	8703-8707	IN	denotes	with
T23911	8688-8692	VBD	denotes	were
T23910	8693-8702	VBN	denotes	re-probed
T23909	8682-8687	NNS	denotes	blots
T23908	8678-8681	DT	denotes	All
T23907	8677-8788	sentence	denotes	All blots were re-probed with an actin antibody to allow amount of protein loaded in each lane to be compared.
T23906	8676-8677	.	denotes	.
T23905	8669-8676	NNS	denotes	tissues
T23904	8663-8668	DT	denotes	those
T23903	8660-8662	IN	denotes	in
T23902	8657-8659	NN	denotes	E2
T23901	8649-8656	VBN	denotes	derived
T23900	8639-8648	NN	denotes	transgene
T23899	8636-8638	IN	denotes	of
T23898	8628-8635	NNS	denotes	amounts
T23897	8617-8627	JJ	denotes	negligible
T23896	8606-8607	-RRB-	denotes	)
T23895	8605-8606	NN	denotes	E
T23894	8604-8605	-LRB-	denotes	(
T23893	8597-8603	NN	denotes	muscle
T23892	8593-8596	CC	denotes	and
T23891	8591-8593	,	denotes	,
T23890	8590-8591	-RRB-	denotes	)
T23889	8589-8590	NN	denotes	D
T23888	8588-8589	-LRB-	denotes	(
T23887	8581-8587	NN	denotes	kidney
T23886	8579-8581	,	denotes	,
T23885	8578-8579	-RRB-	denotes	)
T23884	8577-8578	NN	denotes	C
T23883	8576-8577	-LRB-	denotes	(
T23882	8570-8575	NN	denotes	brain
T23881	8567-8569	IN	denotes	of
T23880	8608-8616	VBD	denotes	revealed
T23879	8558-8566	NN	denotes	analysis
T23878	8553-8557	NN	denotes	blot
T23877	8545-8552	NNP	denotes	Western
T23876	8544-8677	sentence	denotes	Western blot analysis of brain (C), kidney (D), and muscle (E) revealed negligible amounts of transgene derived E2 in those tissues.
T23875	8543-8544	.	denotes	.
T23874	8535-8543	NNS	denotes	controls
T23873	8532-8534	IN	denotes	in
T23872	8528-8531	RB	denotes	not
T23871	8524-8527	CC	denotes	but
T23870	8519-8523	NNS	denotes	mice
T23869	8508-8518	JJ	denotes	transgenic
T23868	8505-8507	IN	denotes	in
T23867	8496-8501	JJ	denotes	human
T23866	8494-8496	,	denotes	,
T23865	8488-8494	VBN	denotes	tagged
T23864	8483-8484	HYPH	denotes	-
T23863	8484-8487	NN	denotes	myc
T23862	8482-8483	NN	denotes	c
T23861	8480-8482	,	denotes	,
T23860	8473-8480	VBN	denotes	derived
T23859	8463-8472	NN	denotes	transgene
T23858	8502-8504	NN	denotes	E2
T23857	8459-8462	DT	denotes	the
T23856	8456-8458	IN	denotes	of
T23855	8447-8455	NN	denotes	presence
T23854	8443-8446	DT	denotes	the
T23853	8420-8423	NN	denotes	tag
T23852	8415-8416	HYPH	denotes	-
T23851	8416-8419	NN	denotes	myc
T23850	8414-8415	NN	denotes	c
T23849	8424-8432	NN	denotes	antibody
T23848	8412-8413	DT	denotes	a
T23847	8407-8411	IN	denotes	with
T23846	8433-8442	VBD	denotes	confirmed
T23845	8398-8399	HYPH	denotes	-
T23844	8399-8406	NN	denotes	probing
T23843	8396-8398	NN	denotes	Re
T23842	8395-8544	sentence	denotes	Re-probing with a c-myc tag antibody confirmed the presence of the transgene derived, c-myc tagged, human E2 in transgenic mice but not in controls.
T23841	8394-8395	.	denotes	.
T23840	8387-8394	NNS	denotes	animals
T23839	8379-8386	NN	denotes	control
T23838	8376-8378	IN	denotes	in
T23837	8374-8375	-RRB-	denotes	)
T23836	8369-8371	CD	denotes	47
T23835	8368-8369	SYM	denotes	~
T23834	8367-8368	SYM	denotes	=
T23833	8365-8367	NN	denotes	MW
T23832	8372-8374	NN	denotes	Kd
T23831	8364-8365	-LRB-	denotes	(
T23830	8361-8363	NN	denotes	E2
T23829	8355-8360	NN	denotes	mouse
T23828	8352-8354	IN	denotes	of
T23827	8345-8351	NN	denotes	amount
T23826	8341-8344	DT	denotes	the
T23825	8338-8340	IN	denotes	to
T23824	8330-8337	JJ	denotes	similar
T23823	8319-8325	NN	denotes	amount
T23822	8315-8318	DT	denotes	the
T23821	8307-8314	NNS	denotes	animals
T23820	8303-8306	DT	denotes	the
T23819	8300-8302	IN	denotes	of
T23818	8295-8299	JJ	denotes	many
T23817	8326-8329	VBD	denotes	was
T23816	8292-8294	IN	denotes	in
T23815	8288-8291	CC	denotes	but
T23814	8279-8287	JJ	denotes	variable
T23813	8273-8274	NN	denotes	A
T23812	8269-8272	CD	denotes	525
T23811	8265-8268	CC	denotes	and
T23810	8263-8264	NN	denotes	A
T23809	8257-8262	NNS	denotes	Lines
T23808	8252-8256	IN	denotes	from
T23807	8247-8251	NNS	denotes	mice
T23806	8244-8246	IN	denotes	in
T23805	8242-8243	-RRB-	denotes	)
T23804	8240-8242	NN	denotes	Kd
T23803	8237-8239	CD	denotes	54
T23802	8236-8237	SYM	denotes	~
T23801	8223-8232	VBN	denotes	predicted
T23800	8233-8235	NN	denotes	MW
T23799	8222-8223	-LRB-	denotes	(
T23798	8211-8213	NN	denotes	E2
T23797	8214-8221	NN	denotes	protein
T23796	8205-8210	JJ	denotes	human
T23795	8202-8204	IN	denotes	of
T23794	8195-8201	NN	denotes	amount
T23793	8191-8194	DT	denotes	the
T23792	8275-8278	VBD	denotes	was
T23791	8186-8190	IN	denotes	that
T23790	8181-8185	VB	denotes	Note
T23789	8180-8395	sentence	denotes	Note that the amount of human E2 protein (predicted MW ~54 Kd) in mice from Lines A and 525 A was variable but in many of the animals the amount was similar to the amount of mouse E2 (MW=~47 Kd) in control animals.
T23788	8179-8180	.	denotes	.
T23787	8170-8174	NN	denotes	MSUD
T23786	8157-8169	JJ	denotes	intermediate
T23785	8153-8156	CC	denotes	and
T23784	8175-8179	NNS	denotes	mice
T23783	8145-8152	NN	denotes	control
T23782	8142-8144	IN	denotes	of
T23781	8136-8141	NN	denotes	liver
T23780	8133-8135	IN	denotes	in
T23779	8125-8132	NN	denotes	protein
T23778	8122-8124	NN	denotes	E2
T23777	8119-8121	IN	denotes	of
T23776	8105-8109	NN	denotes	blot
T23775	8097-8104	NNP	denotes	Western
T23774	8095-8097	,	denotes	,
T23773	8110-8118	NN	denotes	analysis
T23772	8094-8095	LS	denotes	B
T23771	8093-8180	sentence	denotes	B, Western blot analysis of E2 protein in liver of control and intermediate MSUD mice.
T23770	8092-8093	.	denotes	.
T23769	8088-8092	NNS	denotes	mice
T23768	8077-8087	JJ	denotes	transgenic
T23767	8074-8076	IN	denotes	of
T23766	8068-8073	NNS	denotes	lines
T23765	8060-8067	JJ	denotes	several
T23764	8053-8059	VB	denotes	create
T23763	8050-8052	TO	denotes	to
T23762	8041-8044	VBD	denotes	was
T23761	8045-8049	VBN	denotes	used
T23760	8031-8040	NN	denotes	construct
T23759	8026-8030	DT	denotes	This
T23758	8025-8093	sentence	denotes	This construct was used to create several lines of transgenic mice.
T23757	8024-8025	.	denotes	.
T23756	8000-8015	NN	denotes	polyadenylation
T23755	8016-8024	NN	denotes	sequence
T23754	7992-7999	VBN	denotes	derived
T23753	7987-7991	NN	denotes	SV40
T23752	7983-7986	CC	denotes	and
T23751	7981-7983	,	denotes	,
T23750	7970-7977	NN	denotes	epitope
T23749	7965-7966	HYPH	denotes	-
T23748	7966-7969	NN	denotes	myc
T23747	7964-7965	NN	denotes	c
T23746	7978-7981	NN	denotes	tag
T23745	7962-7963	DT	denotes	a
T23744	7960-7962	,	denotes	,
T23743	7946-7953	NN	denotes	alanine
T23742	7954-7960	NN	denotes	spacer
T23741	7943-7945	DT	denotes	an
T23740	7941-7943	,	denotes	,
T23739	7934-7936	NN	denotes	E2
T23738	7928-7933	JJ	denotes	human
T23737	7937-7941	NN	denotes	cDNA
T23736	7924-7927	DT	denotes	the
T23735	7922-7924	,	denotes	,
T23734	7921-7922	-RRB-	denotes	)
T23733	7912-7916	JJ	denotes	thin
T23732	7917-7921	NN	denotes	line
T23731	7911-7912	-LRB-	denotes	(
T23730	7894-7903	JJ	denotes	synthetic
T23729	7904-7910	NN	denotes	intron
T23728	7892-7893	DT	denotes	a
T23727	7890-7892	,	denotes	,
T23726	7882-7890	NN	denotes	promoter
T23725	7878-7881	DT	denotes	the
T23724	7875-7877	IN	denotes	of
T23723	7870-7874	NN	denotes	part
T23722	7867-7869	IN	denotes	as
T23721	7865-7866	-RRB-	denotes	)
T23720	7862-7865	NN	denotes	TRE
T23719	7861-7862	-LRB-	denotes	(
T23718	7844-7852	NN	denotes	response
T23717	7831-7843	NN	denotes	tetracycline
T23716	7853-7860	NN	denotes	element
T23715	7827-7830	DT	denotes	the
T23714	7818-7826	VBZ	denotes	contains
T23713	7804-7805	HYPH	denotes	-
T23712	7805-7807	NN	denotes	E2
T23711	7801-7804	NN	denotes	TRE
T23710	7808-7817	NN	denotes	transgene
T23709	7797-7800	DT	denotes	The
T23708	7796-8025	sentence	denotes	The TRE-E2 transgene contains the tetracycline response element (TRE) as part of the promoter, a synthetic intron (thin line), the human E2 cDNA, an alanine spacer, a c-myc epitope tag, and SV40 derived polyadenylation sequence.
T23707	7795-7796	.	denotes	.
T23706	7783-7786	NN	denotes	LAP
T23705	7774-7782	JJ	denotes	specific
T23704	7768-7773	NN	denotes	liver
T23703	7787-7795	NN	denotes	promoter
T23702	7764-7767	DT	denotes	the
T23701	7759-7763	IN	denotes	from
T23700	7757-7758	-RRB-	denotes	)
T23699	7754-7757	NN	denotes	tTA
T23698	7753-7754	-LRB-	denotes	(
T23697	7726-7727	HYPH	denotes	-
T23696	7727-7737	VBN	denotes	controlled
T23695	7714-7726	NN	denotes	tetracycline
T23694	7738-7752	NN	denotes	transactivator
T23693	7710-7713	DT	denotes	the
T23692	7702-7709	VBP	denotes	express
T23691	7697-7701	NNS	denotes	mice
T23690	7691-7696	DT	denotes	These
T23689	7690-7796	sentence	denotes	These mice express the tetracycline-controlled transactivator (tTA) from the liver specific LAP promoter.
T23688	7689-7690	.	denotes	.
T23687	7688-7689	-RRB-	denotes	]
T23686	7686-7688	CD	denotes	34
T23685	7685-7686	-LRB-	denotes	[
T23684	7664-7674	RB	denotes	previously
T23683	7659-7663	VBN	denotes	been
T23682	7654-7658	VBP	denotes	have
T23681	7675-7684	VBN	denotes	described
T23680	7638-7648	JJ	denotes	transgenic
T23679	7649-7653	NNS	denotes	mice
T23678	7633-7634	HYPH	denotes	-
T23677	7634-7637	NN	denotes	tTA
T23676	7630-7633	NN	denotes	LAP
T23675	7629-7690	sentence	denotes	LAP-tTA transgenic mice have been previously described [34].
T23674	7628-7629	.	denotes	.
T23673	7626-7628	NN	denotes	E2
T23672	7620-7625	JJ	denotes	human
T23671	7612-7619	VBP	denotes	express
T23670	7607-7611	WDT	denotes	that
T23669	7602-7606	NNS	denotes	mice
T23668	7594-7601	VB	denotes	produce
T23667	7591-7593	TO	denotes	to
T23666	7586-7590	VBN	denotes	used
T23665	7566-7576	JJ	denotes	Transgenic
T23664	7564-7566	,	denotes	,
T23663	7577-7585	NN	denotes	strategy
T23662	7563-7564	LS	denotes	A
T23661	7562-7629	sentence	denotes	A, Transgenic strategy used to produce mice that express human E2.
T23660	7561-7562	.	denotes	.
T23659	7545-7561	NN	denotes	characterization
T23658	7541-7544	CC	denotes	and
T23657	7524-7529	NN	denotes	mouse
T23656	7530-7540	NN	denotes	production
T23655	7513-7523	JJ	denotes	Transgenic
T16742	14637-14638	-LRB-	denotes	[
T16825	15014-15015	.	denotes	.
T16824	15007-15014	NNS	denotes	tissues
T16823	15001-15006	DT	denotes	those
T16822	14998-15000	IN	denotes	in
T16821	14987-14997	JJ	denotes	negligible
T16820	14981-14983	NN	denotes	E2
T16819	14978-14980	IN	denotes	of
T16818	14967-14977	NN	denotes	expression
T16817	14965-14967	,	denotes	,
T16816	14953-14965	RB	denotes	respectively
T16815	14950-14952	NN	denotes	3E
T16814	14946-14949	CC	denotes	and
T16813	14944-14945	NN	denotes	D
T16812	14942-14944	,	denotes	,
T16811	14940-14942	NN	denotes	3C
T16810	14933-14939	NN	denotes	Figure
T16809	14930-14932	IN	denotes	in
T16808	14984-14986	VBZ	denotes	is
T16807	14924-14929	VBN	denotes	shown
T16806	14921-14923	IN	denotes	As
T16805	14920-15015	sentence	denotes	As shown in Figure 3C, D and 3E respectively, expression of E2 is negligible in those tissues.
T16804	14919-14920	.	denotes	.
T16803	14913-14919	NN	denotes	muscle
T16802	14909-14912	CC	denotes	and
T16801	14902-14908	NN	denotes	kidney
T16800	14900-14902	,	denotes	,
T16799	14895-14900	NN	denotes	brain
T16798	14892-14894	IN	denotes	in
T16797	14879-14881	NN	denotes	E2
T16796	14882-14891	NN	denotes	transgene
T16795	14875-14878	DT	denotes	the
T16794	14872-14874	IN	denotes	of
T16793	14861-14871	NN	denotes	expression
T16792	14853-14860	VB	denotes	analyze
T16791	14850-14852	TO	denotes	to
T16790	14841-14849	NN	denotes	analysis
T16789	14836-14840	NN	denotes	blot
T16788	14828-14835	NNP	denotes	western
T16787	14818-14822	RB	denotes	also
T16786	14823-14827	VBD	denotes	used
T16785	14815-14817	PRP	denotes	We
T16784	14814-14920	sentence	denotes	We also used western blot analysis to analyze expression of the E2 transgene in brain, kidney and muscle.
T16783	14813-14814	.	denotes	.
T16782	14803-14807	NN	denotes	acid
T16781	14797-14802	NN	denotes	amino
T16780	14808-14813	NN	denotes	level
T16779	14793-14796	DT	denotes	the
T16778	14790-14792	IN	denotes	at
T16777	14787-14789	NN	denotes	E2
T16776	14781-14786	NN	denotes	mouse
T16775	14778-14780	IN	denotes	to
T16774	14769-14777	NN	denotes	identity
T16773	14767-14768	NN	denotes	%
T16772	14765-14767	CD	denotes	88
T16771	14764-14765	SYM	denotes	~
T16770	14757-14763	VBZ	denotes	shares
T16769	14754-14756	NN	denotes	E2
T16768	14748-14753	JJ	denotes	Human
T16767	14747-14814	sentence	denotes	Human E2 shares ~88% identity to mouse E2 at the amino acid level.
T16766	14746-14747	.	denotes	.
T16765	14738-14746	NNS	denotes	subunits
T16764	14735-14737	NN	denotes	E3
T16763	14731-14734	CC	denotes	and
T16762	14728-14730	NN	denotes	E1
T16761	14722-14727	NN	denotes	mouse
T16760	14717-14721	IN	denotes	with
T16759	14707-14716	VBN	denotes	complexed
T16758	14702-14706	WRB	denotes	when
T16757	14691-14701	JJ	denotes	functional
T16756	14685-14690	RB	denotes	fully
T16755	14681-14684	RB	denotes	not
T16754	14674-14676	NN	denotes	E2
T16753	14668-14673	JJ	denotes	human
T16752	14677-14680	VBD	denotes	was
T16751	14663-14667	IN	denotes	that
T16750	14654-14662	JJ	denotes	possible
T16749	14649-14653	RB	denotes	also
T16748	14646-14648	VBZ	denotes	is
T16747	14643-14645	PRP	denotes	It
T16746	14642-14747	sentence	denotes	It is also possible that human E2 was not fully functional when complexed with mouse E1 and E3 subunits.
T16745	14641-14642	.	denotes	.
T16744	14640-14641	-RRB-	denotes	]
T16743	14638-14640	CD	denotes	36
T16741	14620-14627	NN	denotes	subunit
T16740	14628-14636	NN	denotes	assembly
T16739	14613-14619	JJ	denotes	normal
T16738	14608-14612	IN	denotes	with
T16737	14584-14588	FW	denotes	coli
T16736	14581-14583	FW	denotes	E.
T16735	14571-14580	JJ	denotes	analogous
T16734	14589-14596	NN	denotes	subunit
T16733	14568-14570	DT	denotes	an
T16732	14565-14567	IN	denotes	of
T16731	14548-14555	NN	denotes	carboxy
T16730	14556-14564	NN	denotes	terminus
T16729	14544-14547	DT	denotes	the
T16728	14541-14543	IN	denotes	on
T16727	14531-14536	NN	denotes	Hisx6
T16726	14537-14540	NN	denotes	tag
T16725	14529-14530	DT	denotes	a
T16724	14526-14528	IN	denotes	of
T16723	14597-14607	VBD	denotes	interfered
T16722	14516-14525	NN	denotes	insertion
T16721	14512-14515	CC	denotes	and
T16720	14510-14511	-RRB-	denotes	]
T16719	14508-14510	CD	denotes	35
T16718	14507-14508	-LRB-	denotes	[
T16717	14494-14506	NNS	denotes	interactions
T16716	14486-14493	NN	denotes	subunit
T16715	14482-14485	IN	denotes	for
T16714	14472-14481	JJ	denotes	essential
T16713	14447-14460	NN	denotes	dehydrogenase
T16712	14438-14446	NN	denotes	pyruvate
T16711	14461-14468	NN	denotes	complex
T16710	14434-14437	DT	denotes	the
T16709	14431-14433	IN	denotes	of
T16708	14413-14422	JJ	denotes	analogous
T16707	14423-14430	NN	denotes	subunit
T16706	14410-14412	DT	denotes	an
T16705	14407-14409	IN	denotes	of
T16704	14390-14397	NN	denotes	carboxy
T16703	14398-14406	NN	denotes	terminus
T16702	14386-14389	DT	denotes	the
T16701	14469-14471	VBZ	denotes	is
T16700	14381-14385	IN	denotes	that
T16699	14367-14371	VBP	denotes	have
T16698	14372-14380	VBN	denotes	revealed
T16697	14361-14366	WDT	denotes	which
T16696	14353-14360	NNS	denotes	studies
T16695	14344-14352	JJ	denotes	previous
T16694	14339-14343	IN	denotes	with
T16693	14328-14338	JJ	denotes	consistent
T16692	14325-14327	VBZ	denotes	is
T16691	14310-14324	NN	denotes	interpretation
T16690	14305-14309	DT	denotes	This
T16689	14304-14642	sentence	denotes	This interpretation is consistent with previous studies which have revealed that the carboxy terminus of an analogous subunit of the pyruvate dehydrogenase complex is essential for subunit interactions [35] and insertion of a Hisx6 tag on the carboxy terminus of an analogous E. coli subunit interfered with normal subunit assembly [36].
T16688	14303-14304	.	denotes	.
T16687	14295-14303	NN	denotes	activity
T16686	14285-14294	JJ	denotes	enzymatic
T16685	14280-14284	IN	denotes	with
T16684	14260-14265	JJ	denotes	human
T16683	14252-14259	VBN	denotes	derived
T16682	14242-14251	NN	denotes	transgene
T16681	14266-14268	NN	denotes	E2
T16680	14238-14241	DT	denotes	the
T16679	14235-14237	IN	denotes	of
T16678	14218-14225	NN	denotes	carboxy
T16677	14226-14234	NN	denotes	terminus
T16676	14214-14217	DT	denotes	the
T16675	14211-14213	IN	denotes	at
T16674	14202-14203	HYPH	denotes	-
T16673	14203-14206	NN	denotes	myc
T16672	14201-14202	NN	denotes	c
T16671	14207-14210	NN	denotes	tag
T16670	14197-14200	DT	denotes	the
T16669	14269-14279	VBD	denotes	interfered
T16668	14192-14196	IN	denotes	that
T16667	14183-14191	JJ	denotes	probable
T16666	14180-14182	VBZ	denotes	is
T16665	14177-14179	PRP	denotes	It
T16664	14176-14304	sentence	denotes	It is probable that the c-myc tag at the carboxy terminus of the transgene derived human E2 interfered with enzymatic activity.
T16663	14175-14176	.	denotes	.
T16662	14159-14169	JJ	denotes	suboptimal
T16661	14170-14175	NN	denotes	level
T16660	14157-14158	DT	denotes	a
T16659	14154-14156	IN	denotes	at
T16658	14139-14141	VBZ	denotes	is
T16657	14128-14135	VBN	denotes	derived
T16656	14136-14138	NN	denotes	E2
T16655	14118-14127	NN	denotes	transgene
T16654	14142-14153	VBG	denotes	functioning
T16653	14113-14117	IN	denotes	that
T16652	14089-14095	NN	denotes	enzyme
T16651	14083-14088	NN	denotes	BCKDH
T16650	14096-14104	NN	denotes	activity
T16649	14076-14082	JJ	denotes	normal
T16648	14073-14075	IN	denotes	of
T16647	14071-14072	NN	denotes	%
T16646	14069-14070	SYM	denotes	–
T16645	14068-14069	CD	denotes	5
T16644	14067-14068	SYM	denotes	~
T16643	14070-14071	CD	denotes	6
T16642	14062-14066	RB	denotes	only
T16641	14059-14061	IN	denotes	in
T16640	14044-14051	NN	denotes	protein
T16639	14041-14043	NN	denotes	E2
T16638	14038-14040	IN	denotes	of
T16637	14023-14029	JJ	denotes	normal
T16636	14018-14022	RB	denotes	near
T16635	14030-14037	NNS	denotes	amounts
T16634	14012-14017	DT	denotes	these
T16633	14052-14058	VBP	denotes	result
T16632	14007-14011	IN	denotes	that
T16631	14105-14112	VBP	denotes	suggest
T16630	13994-14006	NNS	denotes	observations
T16629	13990-13993	DT	denotes	The
T16628	13989-14176	sentence	denotes	The observations that these near normal amounts of E2 protein result in only ~5–6% of normal BCKDH enzyme activity suggest that transgene derived E2 is functioning at a suboptimal level.
T16627	13988-13989	.	denotes	.
T16626	13976-13983	NN	denotes	control
T16625	13984-13988	NNS	denotes	mice
T16624	13962-13975	JJ	denotes	nontransgenic
T16623	13959-13961	IN	denotes	of
T16622	13953-13958	NN	denotes	liver
T16621	13950-13952	IN	denotes	in
T16620	13941-13949	VBN	denotes	produced
T16619	13939-13940	-RRB-	denotes	)
T16618	13934-13936	CD	denotes	47
T16617	13933-13934	SYM	denotes	~
T16616	13937-13939	NN	denotes	Kd
T16615	13932-13933	-LRB-	denotes	(
T16614	13929-13931	NN	denotes	E2
T16613	13923-13928	NN	denotes	mouse
T16612	13920-13922	IN	denotes	of
T16611	13913-13919	NN	denotes	amount
T16610	13909-13912	DT	denotes	the
T16529	13542-13545	VBD	denotes	was
T16528	13537-13541	NN	denotes	525A
T16527	13533-13536	CC	denotes	and
T16526	13531-13532	NN	denotes	A
T16525	13525-13530	NNS	denotes	Lines
T16524	13522-13524	IN	denotes	of
T16523	13516-13521	NN	denotes	liver
T16522	13513-13515	IN	denotes	in
T16521	13502-13504	NN	denotes	E2
T16520	13505-13512	NN	denotes	protein
T16519	13496-13501	JJ	denotes	human
T16518	13493-13495	IN	denotes	of
T16517	13482-13492	NN	denotes	production
T16516	13478-13481	CC	denotes	and
T16515	13462-13468	NN	denotes	enzyme
T16514	13469-13477	NN	denotes	activity
T16513	13456-13461	NN	denotes	BCKDH
T16512	13454-13456	,	denotes	,
T16511	13453-13454	-RRB-	denotes	]
T16510	13451-13453	CD	denotes	34
T16509	13450-13451	-LRB-	denotes	[
T16508	13439-13449	NN	denotes	expression
T16507	13430-13438	JJ	denotes	specific
T16506	13424-13429	NN	denotes	liver
T16505	13416-13423	VB	denotes	produce
T16504	13413-13415	TO	denotes	to
T16503	13546-13554	VBN	denotes	examined
T16502	13395-13399	VBN	denotes	been
T16482	12660-12661	sentence	denotes
T16481	12659-12660	.	denotes	.
T16480	12658-12659	-RRB-	denotes	)
T16479	12657-12658	CD	denotes	2
T16478	12651-12656	NN	denotes	Table
T16477	12650-12651	-LRB-	denotes	(
T16476	12641-12649	NNS	denotes	controls
T16475	12638-12640	IN	denotes	to
T16474	12629-12637	VBN	denotes	compared
T16473	12627-12629	,	denotes	,
T17014	15913-15917	RB	denotes	very
T17013	15925-15930	NN	denotes	model
T17012	15911-15912	DT	denotes	a
T17011	15889-15895	NN	denotes	rescue
T17010	15896-15900	NNS	denotes	mice
T17009	15883-15888	DT	denotes	these
T17008	15881-15883	,	denotes	,
T17007	15901-15910	VBP	denotes	represent
T17006	15877-15881	RB	denotes	Thus
T17005	15876-15966	sentence	denotes	Thus, these rescue mice represent a very useful model of the intermediate MSUD phenotype.
T17004	15875-15876	.	denotes	.
T17003	15874-15875	-RRB-	denotes	]
T17002	15873-15874	CD	denotes	1
T17001	15872-15873	-LRB-	denotes	[
T17000	15864-15871	NN	denotes	disease
T16999	15860-15863	DT	denotes	the
T16998	15857-15859	IN	denotes	of
T16997	15839-15851	JJ	denotes	intermediate
T16996	15852-15856	NN	denotes	form
T16995	15835-15838	DT	denotes	the
T16994	15830-15834	IN	denotes	with
T16993	15821-15829	NNS	denotes	patients
T16992	15816-15820	NN	denotes	MSUD
T16991	15813-15815	IN	denotes	in
T16990	15804-15812	VBN	denotes	observed
T16989	15785-15793	JJ	denotes	clinical
T16988	15794-15803	NN	denotes	phenotype
T16987	15781-15784	DT	denotes	the
T16986	15778-15780	IN	denotes	to
T16985	15770-15777	JJ	denotes	similar
T16984	15759-15769	RB	denotes	remarkably
T16983	15755-15758	VBP	denotes	are
T16982	15731-15741	JJ	denotes	phenotypic
T16981	15742-15754	NNS	denotes	observations
T16980	15725-15730	DT	denotes	These
T16979	15724-15876	sentence	denotes	These phenotypic observations are remarkably similar to the clinical phenotype observed in MSUD patients with the intermediate form of the disease [1].
T16978	15723-15724	.	denotes	.
T16977	15718-15723	NN	denotes	liver
T16976	15714-15717	DT	denotes	the
T16975	15711-15713	IN	denotes	in
T16974	15695-15701	NN	denotes	enzyme
T16973	15689-15694	NN	denotes	BCKDH
T16972	15702-15710	NN	denotes	activity
T16971	15682-15688	JJ	denotes	normal
T16970	15679-15681	IN	denotes	of
T16969	15677-15678	NN	denotes	%
T16968	15675-15676	SYM	denotes	–
T16967	15674-15675	CD	denotes	5
T16966	15676-15677	CD	denotes	6
T16965	15673-15674	SYM	denotes	~
T16964	15663-15672	VBD	denotes	expressed
T16963	15658-15662	PRP	denotes	they
T16962	15654-15657	CC	denotes	and
T16961	15644-15653	NNS	denotes	knockouts
T16960	15640-15643	CC	denotes	and
T16959	15631-15639	NNS	denotes	controls
T16958	15623-15630	IN	denotes	between
T16957	15610-15622	JJ	denotes	intermediate
T16956	15605-15609	VBD	denotes	were
T16955	15600-15604	WDT	denotes	that
T16954	15593-15599	NNS	denotes	ratios
T16953	15584-15585	HYPH	denotes	/
T16952	15585-15592	NN	denotes	alanine
T16951	15580-15584	NN	denotes	BCAA
T16950	15561-15570	VBG	denotes	surviving
T16949	15571-15575	NNS	denotes	mice
T16948	15555-15560	DT	denotes	these
T16947	15553-15555	,	denotes	,
T16946	15546-15553	NN	denotes	summary
T16945	15576-15579	VBD	denotes	had
T16944	15543-15545	IN	denotes	In
T16943	15542-15724	sentence	denotes	In summary, these surviving mice had BCAA/alanine ratios that were intermediate between controls and knockouts and they expressed ~5–6% of normal BCKDH enzyme activity in the liver.
T16942	15541-15542	.	denotes	.
T16941	15538-15541	NN	denotes	age
T16940	15535-15537	IN	denotes	of
T16939	15526-15528	CD	denotes	60
T16938	15522-15525	CC	denotes	and
T16937	15529-15534	NNS	denotes	weeks
T16936	15519-15521	CD	denotes	40
T16935	15516-15518	IN	denotes	at
T16934	15511-15515	VBD	denotes	died
T16933	15496-15500	JJ	denotes	rare
T16932	15492-15495	CD	denotes	two
T16931	15501-15510	NNS	denotes	survivors
T16930	15486-15491	DT	denotes	These
T16929	15485-15542	sentence	denotes	These two rare survivors died at 40 and 60 weeks of age.
T16928	15484-15485	.	denotes	.
T16927	15479-15484	JJ	denotes	alive
T16926	15473-15478	RB	denotes	still
T16925	15466-15467	-RRB-	denotes	)
T16924	15464-15466	CD	denotes	16
T16923	15461-15463	IN	denotes	of
T16922	15459-15460	CD	denotes	2
T16921	15458-15459	-LRB-	denotes	(
T16920	15453-15457	NNS	denotes	mice
T16919	15450-15452	IN	denotes	of
T16918	15448-15449	NN	denotes	%
T16917	15446-15448	CD	denotes	12
T16916	15445-15446	SYM	denotes	~
T16915	15443-15445	,	denotes	,
T16914	15440-15443	NN	denotes	age
T16913	15437-15439	IN	denotes	of
T16912	15431-15436	NNS	denotes	weeks
T16911	15428-15430	CD	denotes	20
T16910	15468-15472	VBD	denotes	were
T16909	15425-15427	IN	denotes	At
T16908	15424-15485	sentence	denotes	At 20 weeks of age, ~12% of mice (2 of 16) were still alive.
T16907	15423-15424	.	denotes	.
T16906	15417-15423	JJR	denotes	better
T16905	15408-15416	RB	denotes	somewhat
T16904	15405-15407	VB	denotes	be
T16903	15402-15404	TO	denotes	to
T16902	15388-15392	NN	denotes	525A
T16901	15383-15387	NN	denotes	Line
T16900	15380-15382	IN	denotes	of
T16899	15393-15401	VBD	denotes	appeared
T16898	15371-15379	NN	denotes	Survival
T16897	15370-15424	sentence	denotes	Survival of Line 525A appeared to be somewhat better.
T16896	15369-15370	.	denotes	.
T16895	15365-15369	NN	denotes	cage
T16894	15359-15364	PRP$	denotes	their
T16893	15356-15358	IN	denotes	in
T16892	15351-15355	JJ	denotes	dead
T16891	15345-15350	VBN	denotes	found
T16890	15340-15344	VBD	denotes	were
T16889	15337-15339	CC	denotes	or
T16888	15335-15337	,	denotes	,
T16887	15325-15335	VBN	denotes	sacrificed
T16886	15316-15324	RB	denotes	humanely
T16885	15312-15315	CC	denotes	and
T16884	15303-15311	JJ	denotes	moribund
T16883	15296-15297	NN	denotes	A
T16882	15291-15295	NN	denotes	Line
T16881	15288-15290	IN	denotes	of
T16880	15283-15287	NNS	denotes	mice
T16879	15279-15282	DT	denotes	all
T16878	15277-15279	,	denotes	,
T16877	15272-15277	NNS	denotes	weeks
T16876	15269-15271	CD	denotes	16
T16875	15298-15302	VBD	denotes	were
T16874	15266-15268	IN	denotes	By
T16873	15265-15370	sentence	denotes	By 16 weeks, all mice of Line A were moribund and humanely sacrificed, or were found dead in their cage.
T16872	15264-15265	.	denotes	.
T16871	15249-15252	VBD	denotes	was
T16870	15237-15243	NN	denotes	rescue
T16869	15244-15248	NNS	denotes	mice
T16868	15233-15236	DT	denotes	the
T16867	15230-15232	IN	denotes	of
T16866	15221-15229	NN	denotes	survival
T16865	15253-15264	VBN	denotes	compromised
T16864	15216-15220	IN	denotes	that
T16863	15207-15215	JJ	denotes	apparent
T16862	15199-15206	RB	denotes	readily
T16861	15193-15195	PRP	denotes	it
T16860	15191-15193	,	denotes	,
T16859	15196-15198	VBZ	denotes	is
T16858	15178-15181	RB	denotes	not
T16857	15174-15177	VBD	denotes	was
T16856	15166-15173	NNS	denotes	animals
T16855	15158-15165	NN	denotes	control
T16854	15154-15157	IN	denotes	for
T16853	15149-15153	NNS	denotes	data
T16852	15140-15148	NN	denotes	survival
T16851	15182-15191	VBN	denotes	collected
T16850	15131-15139	IN	denotes	Although
T16849	15130-15265	sentence	denotes	Although survival data for control animals was not collected, it is readily apparent that survival of the rescue mice was compromised.
T16848	15129-15130	.	denotes	.
T16847	15127-15129	NN	denotes	4D
T16846	15122-15126	NN	denotes	Fig.
T16845	15119-15121	IN	denotes	in
T16844	15108-15110	VBZ	denotes	is
T16843	15100-15107	NN	denotes	weaning
T16842	15093-15099	IN	denotes	beyond
T16841	15084-15092	VBD	denotes	survived
T16840	15079-15083	WDT	denotes	that
T16839	15062-15072	JJ	denotes	transgenic
T16838	15058-15061	CD	denotes	two
T16837	15073-15078	NNS	denotes	lines
T16836	15052-15057	DT	denotes	these
T16835	15047-15051	IN	denotes	from
T16834	15042-15046	NNS	denotes	mice
T16833	15038-15041	DT	denotes	the
T16832	15035-15037	IN	denotes	of
T16831	15111-15118	VBN	denotes	plotted
T16830	15026-15034	NN	denotes	survival
T16829	15020-15021	HYPH	denotes	-
T16828	15021-15025	NN	denotes	term
T16827	15016-15020	JJ	denotes	Long
T16826	15015-15130	sentence	denotes	Long-term survival of the mice from these two transgenic lines that survived beyond weaning is plotted in Fig. 4D.
T16472	12623-12627	NNS	denotes	mice
T16471	12618-12622	NN	denotes	525A
T16470	12613-12617	NN	denotes	Line
T16469	12610-12612	IN	denotes	in
T16468	12600-12609	NN	denotes	glutamate
T16467	12596-12599	VBD	denotes	was
T16466	12593-12595	IN	denotes	as
T16465	12591-12593	,	denotes	,
T16464	12587-12591	NNS	denotes	mice
T16463	12585-12586	NN	denotes	A
T16462	12580-12584	NN	denotes	Line
T16461	12577-12579	IN	denotes	in
T16460	12564-12568	VBD	denotes	were
T16459	12554-12563	NN	denotes	glutamine
T16458	12550-12553	CC	denotes	and
T16457	12548-12550	,	denotes	,
T16456	12539-12548	NN	denotes	glutamate
T16455	12537-12539	,	denotes	,
T16454	12530-12537	NN	denotes	alanine
T16453	12527-12529	IN	denotes	of
T16452	12569-12576	VBN	denotes	reduced
T16451	12520-12526	NNS	denotes	levels
T16450	12514-12519	NN	denotes	Blood
T16449	12513-12660	sentence	denotes	Blood levels of alanine, glutamate, and glutamine were reduced in Line A mice, as was glutamate in Line 525A mice, compared to controls (Table 2).
T16448	12512-12513	.	denotes	.
T16447	12511-12512	-RRB-	denotes	)
T16446	12509-12511	NN	denotes	2C
T16445	12502-12508	NN	denotes	Figure
T16444	12498-12501	VB	denotes	see
T16443	12497-12498	-LRB-	denotes	(
T16442	12487-12496	NNS	denotes	knockouts
T16441	12483-12486	CC	denotes	and
T16440	12474-12482	NNS	denotes	controls
T16439	12466-12473	IN	denotes	between
T16438	12453-12465	JJ	denotes	intermediate
T16437	12433-12443	JJ	denotes	transgenic
T16436	12444-12448	NNS	denotes	mice
T16435	12429-12432	DT	denotes	the
T16434	12425-12428	IN	denotes	for
T16433	12409-12410	HYPH	denotes	/
T16432	12410-12417	NN	denotes	alanine
T16431	12405-12409	NN	denotes	BCAA
T16430	12418-12424	NNS	denotes	values
T16429	12401-12404	DT	denotes	the
T16428	12449-12452	VBP	denotes	are
T16427	12396-12400	IN	denotes	that
T16426	12391-12395	VB	denotes	Note
T16425	12390-12513	sentence	denotes	Note that the BCAA/alanine values for the transgenic mice are intermediate between controls and knockouts (see Figure 2C).
T16424	12389-12390	.	denotes	.
T16423	12381-12389	VBN	denotes	analyzed
T16422	12369-12373	NN	denotes	time
T16421	12374-12380	NNS	denotes	points
T16420	12365-12368	DT	denotes	the
T16419	12362-12364	IN	denotes	of
T16418	12358-12361	DT	denotes	all
T16417	12354-12357	IN	denotes	for
T16416	12345-12353	NNS	denotes	controls
T16415	12342-12344	IN	denotes	to
T16414	12333-12341	VBN	denotes	compared
T16413	12324-12332	JJ	denotes	elevated
T16412	12310-12323	RB	denotes	significantly
T16411	12299-12304	NNS	denotes	lines
T16410	12294-12298	DT	denotes	both
T16409	12289-12293	IN	denotes	from
T16408	12284-12288	NNS	denotes	mice
T16407	12280-12283	IN	denotes	for
T16406	12305-12309	VBD	denotes	were
T16405	12264-12265	HYPH	denotes	/
T16404	12265-12272	NN	denotes	alanine
T16403	12260-12264	NN	denotes	BCAA
T16402	12273-12279	NNS	denotes	values
T16401	12256-12259	DT	denotes	The
T16400	12255-12390	sentence	denotes	The BCAA/alanine values for mice from both lines were significantly elevated compared to controls for all of the time points analyzed.
T16399	12254-12255	.	denotes	.
T16398	12247-12254	NN	denotes	alanine
T16397	12244-12246	IN	denotes	to
T16396	12239-12243	NN	denotes	BCAA
T16395	12233-12238	JJ	denotes	total
T16394	12230-12232	IN	denotes	of
T16393	12224-12229	NN	denotes	ratio
T16392	12222-12223	DT	denotes	a
T16391	12219-12221	IN	denotes	as
T16390	12209-12218	VBN	denotes	presented
T16389	12195-12199	NN	denotes	acid
T16388	12189-12194	NN	denotes	amino
T16387	12200-12208	NN	denotes	analysis
T16386	12183-12188	NN	denotes	blood
T16385	12180-12182	IN	denotes	of
T16384	12172-12179	NNS	denotes	results
T16383	12168-12171	DT	denotes	the
T16337	12092-12093	sentence	denotes	L
T16336	10923-10924	sentence	denotes
T16335	10922-10923	.	denotes	.
T16334	10918-10922	NNS	denotes	pups
T16333	10911-10917	VBN	denotes	weaned
T16332	10908-10910	IN	denotes	of
T16331	10905-10906	CD	denotes	5
T16330	10901-10904	CC	denotes	and
T16329	10906-10907	NN	denotes	%
T16328	10899-10900	CD	denotes	1
T16327	10891-10898	IN	denotes	between
T16326	10881-10890	NN	denotes	frequency
T16325	10879-10880	DT	denotes	a
T16324	10876-10878	IN	denotes	at
T16323	10861-10867	NN	denotes	rescue
T16322	10868-10875	NNS	denotes	animals
T16277	10617-10618	.	denotes	.
T16276	10602-10608	NN	denotes	rescue
T16275	10609-10617	NN	denotes	genotype
T16274	10598-10601	DT	denotes	the
T16273	10589-10593	NN	denotes	line
T16272	10584-10588	DT	denotes	this
T16271	10579-10583	IN	denotes	from
T16270	10564-10573	VBG	denotes	surviving
T16269	10562-10564	,	denotes	,
T16268	10574-10578	NNS	denotes	pups
T16267	10556-10562	VBN	denotes	weaned
T16266	10553-10555	IN	denotes	of
T16265	10594-10597	VBD	denotes	had
T16264	10551-10552	NN	denotes	%
T16263	10549-10551	CD	denotes	10
T16262	10535-10548	RB	denotes	Approximately
T16261	10534-10618	sentence	denotes	Approximately 10% of weaned, surviving pups from this line had the rescue genotype.
T16260	10533-10534	.	denotes	.
T16259	10528-10531	CD	denotes	525
T16258	10532-10533	NN	denotes	A
T16257	10523-10527	NN	denotes	line
T16256	10511-10518	NN	denotes	weaning
T16255	10508-10510	IN	denotes	at
T16254	10503-10507	NNS	denotes	mice
T16253	10496-10502	NN	denotes	rescue
T16252	10493-10495	IN	denotes	of
T16251	10474-10481	JJS	denotes	highest
T16250	10482-10492	NN	denotes	percentage
T16249	10470-10473	DT	denotes	the
T16248	10465-10469	IN	denotes	with
T16247	10519-10522	VBD	denotes	was
T16246	10460-10464	NN	denotes	line
T16245	10456-10459	DT	denotes	The
T16244	10455-10534	sentence	denotes	The line with the highest percentage of rescue mice at weaning was line 525 A.
T16243	10454-10455	.	denotes	.
T16242	10449-10454	NNS	denotes	lines
T16241	10441-10448	IN	denotes	between
T16240	10428-10431	VBD	denotes	was
T16239	10432-10440	VBN	denotes	observed
T16238	10416-10427	NN	denotes	variability
T16237	10403-10415	JJ	denotes	Considerable
T16236	10402-10455	sentence	denotes	Considerable variability was observed between lines.
T16235	10401-10402	.	denotes	.
T16234	10395-10401	VBN	denotes	tested
T16233	10379-10389	JJ	denotes	transgenic
T16232	10390-10394	NN	denotes	line
T16231	10374-10378	DT	denotes	each
T16230	10369-10373	IN	denotes	from
T16229	10353-10359	NN	denotes	rescue
T16220	10308-10318	NN	denotes	percentage
T17021	15965-15966	.	denotes	.
T17020	15951-15955	NN	denotes	MSUD
T17019	15938-15950	JJ	denotes	intermediate
T17018	15956-15965	NN	denotes	phenotype
T17017	15934-15937	DT	denotes	the
T17016	15931-15933	IN	denotes	of
T16609	13906-13908	IN	denotes	to
T16608	13900-13905	JJ	denotes	equal
T16607	13886-13899	RB	denotes	approximately
T16606	13879-13881	NN	denotes	E2
T16605	13873-13878	JJ	denotes	human
T16604	13870-13872	IN	denotes	of
T16603	13864-13869	NN	denotes	level
T16602	13860-13863	DT	denotes	the
T16601	13858-13860	,	denotes	,
T16600	13843-13853	JJ	denotes	transgenic
T16599	13854-13858	NNS	denotes	mice
T16598	13837-13842	DT	denotes	these
T16597	13834-13836	IN	denotes	of
T16596	13829-13833	DT	denotes	some
T16595	13882-13885	VBD	denotes	was
T16594	13826-13828	IN	denotes	In
T16593	13825-13989	sentence	denotes	In some of these transgenic mice, the level of human E2 was approximately equal to the amount of mouse E2 (~47 Kd) produced in liver of nontransgenic control mice.
T16592	13824-13825	.	denotes	.
T16591	13820-13824	NNS	denotes	mice
T16590	13812-13819	IN	denotes	between
T16589	13803-13811	JJ	denotes	variable
T16588	13797-13802	RB	denotes	quite
T16587	13777-13787	JJ	denotes	transgenic
T16586	13788-13792	NNS	denotes	mice
T16585	13771-13776	DT	denotes	these
T16584	13768-13770	IN	denotes	in
T16583	13766-13767	-RRB-	denotes	)
T16582	13761-13763	NN	denotes	54
T16581	13760-13761	SYM	denotes	~
T16580	13758-13760	NN	denotes	MW
T16579	13748-13757	VBN	denotes	predicted
T16578	13764-13766	NN	denotes	Kd
T16577	13747-13748	-LRB-	denotes	(
T16576	13744-13746	NN	denotes	E2
T16575	13738-13743	JJ	denotes	human
T16574	13735-13737	IN	denotes	of
T16573	13728-13734	NN	denotes	amount
T16572	13724-13727	DT	denotes	the
T16571	13722-13724	,	denotes	,
T16570	13720-13722	NN	denotes	3B
T16569	13715-13719	NNP	denotes	Fig.
T16568	13712-13714	IN	denotes	in
T16567	13793-13796	VBD	denotes	was
T16566	13706-13711	VBN	denotes	shown
T16565	13703-13705	IN	denotes	As
T16564	13702-13825	sentence	denotes	As shown in Fig. 3B, the amount of human E2 (predicted MW~54 Kd) in these transgenic mice was quite variable between mice.
T16563	13701-13702	.	denotes	.
T16562	13700-13701	-RRB-	denotes	)
T16561	13693-13697	NN	denotes	Fig.
T16560	13698-13700	NN	denotes	4C
T16559	13692-13693	-LRB-	denotes	(
T16558	13686-13691	NN	denotes	liver
T16557	13678-13685	NN	denotes	control
T16556	13675-13677	IN	denotes	in
T16555	13667-13674	JJ	denotes	present
T16554	13648-13657	JJ	denotes	enzymatic
T16553	13658-13666	NN	denotes	activity
T16552	13644-13647	DT	denotes	the
T16551	13641-13643	IN	denotes	of
T16550	13639-13641	,	denotes	,
T16549	13627-13639	RB	denotes	respectively
T16548	13625-13627	,	denotes	,
T16547	13623-13624	CD	denotes	5
T16546	13619-13622	CC	denotes	and
T16545	13624-13625	NN	denotes	%
T16544	13617-13618	CD	denotes	6
T16543	13616-13617	SYM	denotes	~
T16542	13607-13611	NN	denotes	525A
T16541	13603-13606	CC	denotes	and
T16540	13601-13602	NN	denotes	A
T16539	13595-13600	NNS	denotes	Lines
T16538	13590-13594	IN	denotes	from
T16537	13584-13589	NN	denotes	liver
T16536	13581-13583	IN	denotes	in
T16535	13612-13615	VBD	denotes	was
T16534	13562-13571	JJ	denotes	enzymatic
T16533	13572-13580	NN	denotes	activity
T16532	13556-13561	NN	denotes	BCKDH
T16531	13555-13702	sentence	denotes	BCKDH enzymatic activity in liver from Lines A and 525A was ~6 and 5%, respectively, of the enzymatic activity present in control liver (Fig. 4C).
T16530	13554-13555	.	denotes	.
T16228	10360-10368	NN	denotes	genotype
T16227	10349-10352	DT	denotes	the
T16226	10344-10348	IN	denotes	with
T16225	10336-10343	NN	denotes	weaning
T16224	10333-10335	IN	denotes	at
T16223	10327-10332	JJ	denotes	alive
T16222	10322-10326	NNS	denotes	mice
T16221	10319-10321	IN	denotes	of
T16219	10304-10307	DT	denotes	the
T16218	10298-10303	VBZ	denotes	shows
T16217	10295-10297	NN	denotes	4A
T16216	10290-10294	NN	denotes	Fig.
T16215	10289-10402	sentence	denotes	Fig. 4A shows the percentage of mice alive at weaning with the rescue genotype from each transgenic line tested.
T16214	10288-10289	.	denotes	.
T16213	10287-10288	NN	denotes	%
T16212	10285-10287	CD	denotes	14
T16211	10282-10284	IN	denotes	of
T16210	10280-10281	-RRB-	denotes	)
T16209	10270-10280	NNS	denotes	transgenes
T16208	10265-10269	DT	denotes	both
T16207	10261-10264	IN	denotes	for
T16206	10252-10260	JJ	denotes	positive
T16205	10248-10251	CC	denotes	and
T16204	10236-10238	NN	denotes	E2
T16203	10225-10235	JJ	denotes	homozygous
T16202	10223-10225	,	denotes	,
T16201	10219-10223	FW	denotes	i.e.
T16200	10239-10247	NN	denotes	knockout
T16199	10218-10219	-LRB-	denotes	(
T16198	10202-10208	NN	denotes	rescue
T16197	10209-10217	NN	denotes	genotype
T16196	10198-10201	DT	denotes	the
T16195	10193-10197	IN	denotes	with
T16194	10185-10192	NNS	denotes	animals
T16193	10182-10184	IN	denotes	of
T16192	10162-10173	JJ	denotes	theoretical
T16191	10174-10181	NN	denotes	maximum
T16190	10160-10161	DT	denotes	a
T16189	10157-10159	IN	denotes	in
T16188	10150-10156	VB	denotes	result
T16187	10147-10149	TO	denotes	to
T16186	10135-10137	VBZ	denotes	is
T16185	10138-10146	VBN	denotes	expected
T16184	10117-10125	VBG	denotes	breeding
T16183	10126-10134	NN	denotes	strategy
T16182	10112-10116	DT	denotes	This
T16181	10111-10289	sentence	denotes	This breeding strategy is expected to result in a theoretical maximum of animals with the rescue genotype (i.e., homozygous E2 knockout and positive for both transgenes) of 14%.
T16180	10110-10111	.	denotes	.
T16179	10103-10110	NN	denotes	weaning
T16178	10100-10102	IN	denotes	at
T16177	10092-10099	NNS	denotes	litters
T16176	10079-10081	PRP	denotes	we
T16175	10077-10079	,	denotes	,
T16174	10068-10077	NN	denotes	lethality
T16173	10059-10067	JJ	denotes	neonatal
T16172	10054-10058	IN	denotes	from
T16171	10045-10053	NN	denotes	knockout
T16170	10041-10044	DT	denotes	the
T16169	10034-10040	VB	denotes	rescue
T16168	10031-10033	TO	denotes	to
T16167	10020-10030	NNS	denotes	transgenes
T16166	10016-10019	DT	denotes	the
T16165	10013-10015	IN	denotes	of
T16164	10005-10012	NN	denotes	ability
T16163	10001-10004	DT	denotes	the
T16162	9997-10000	IN	denotes	for
T16161	10082-10091	VBD	denotes	genotyped
T16160	9978-9989	RB	denotes	efficiently
T16159	9990-9996	VB	denotes	screen
T16158	9975-9977	TO	denotes	To
T16157	9974-10111	sentence	denotes	To efficiently screen for the ability of the transgenes to rescue the knockout from neonatal lethality, we genotyped litters at weaning.
T16156	9973-9974	.	denotes	.
T16155	9962-9964	NN	denotes	E2
T16154	9965-9973	NN	denotes	knockout
T16153	9958-9961	DT	denotes	the
T16152	9954-9957	CC	denotes	and
T16151	9952-9954	,	denotes	,
T16150	9939-9940	HYPH	denotes	-
T16149	9940-9942	NN	denotes	E2
T16148	9936-9939	NN	denotes	TRE
T16147	9943-9952	NN	denotes	transgene
T16146	9932-9935	DT	denotes	the
T16145	9930-9932	,	denotes	,
T16144	9916-9917	HYPH	denotes	-
T16143	9917-9920	NN	denotes	tTA
T16142	9913-9916	NN	denotes	LAP
T16141	9921-9930	NN	denotes	transgene
T16140	9909-9912	DT	denotes	the
T16139	9905-9908	IN	denotes	for
T16138	9892-9904	JJ	denotes	heterozygous
T16137	9882-9886	NNS	denotes	mice
T16136	9878-9881	DT	denotes	the
T16135	9872-9877	WDT	denotes	which
T16134	9887-9891	VBD	denotes	were
T16133	9869-9871	IN	denotes	in
T16132	9859-9863	VBD	denotes	were
T16131	9853-9858	NNS	denotes	pairs
T16130	9844-9852	VBG	denotes	breeding
T16129	9842-9844	,	denotes	,
T16128	9864-9868	VBN	denotes	used
T16127	9832-9842	RB	denotes	Ultimately
T16126	9831-9974	sentence	denotes	Ultimately, breeding pairs were used in which the mice were heterozygous for the LAP-tTA transgene, the TRE-E2 transgene, and the E2 knockout.
T16125	9830-9831	.	denotes	.
T16124	9819-9821	NN	denotes	E2
T16123	9822-9830	NN	denotes	knockout
T16122	9815-9818	DT	denotes	the
T16121	9811-9814	IN	denotes	for
T16120	9798-9810	JJ	denotes	heterozygous
T16119	9793-9797	VBD	denotes	were
T16118	9789-9792	CC	denotes	and
T16117	9774-9775	HYPH	denotes	-
T16116	9775-9778	NN	denotes	tTA
T16115	9771-9774	NN	denotes	LAP
T16114	9779-9788	NN	denotes	transgene
T16113	9767-9770	DT	denotes	the
T16112	9763-9766	IN	denotes	for
T16111	9754-9762	JJ	denotes	positive
T16110	9749-9753	VBD	denotes	were
T16109	9744-9748	WDT	denotes	that
T16108	9739-9743	NNS	denotes	mice
T16107	9736-9738	IN	denotes	to
T16106	9723-9727	VBD	denotes	were
T16105	9721-9722	-RRB-	denotes	)
T16104	9709-9711	NN	denotes	F1
T16103	9706-9708	CC	denotes	or
T16102	9697-9705	NNS	denotes	founders
T16101	9690-9696	CC	denotes	either
T16100	9712-9721	NN	denotes	offspring
T16099	9689-9690	-LRB-	denotes	(
T16098	9684-9688	NN	denotes	line
T16097	9679-9683	DT	denotes	each
T16096	9674-9678	IN	denotes	from
T16095	9728-9735	VBN	denotes	crossed
T16094	9665-9666	HYPH	denotes	-
T16093	9666-9668	NN	denotes	E2
T16092	9662-9665	NN	denotes	TRE
T16091	9669-9673	NNS	denotes	mice
T16090	9651-9661	JJ	denotes	Transgenic
T16089	9650-9831	sentence	denotes	Transgenic TRE-E2 mice from each line (either founders or F1 offspring) were crossed to mice that were positive for the LAP-tTA transgene and were heterozygous for the E2 knockout.
T16088	9649-9650	.	denotes	.
T16087	9648-9649	-RRB-	denotes	)
T16086	9639-9642	RB	denotes	not
T16085	9634-9638	NNS	denotes	data
T16084	9643-9648	VBN	denotes	shown
T16083	9633-9634	-LRB-	denotes	(
T16082	9629-9632	NN	denotes	DNA
T16081	9624-9628	NN	denotes	tail
T16080	9621-9623	IN	denotes	of
T16079	9612-9620	NN	denotes	analysis
T16078	9607-9611	NN	denotes	blot
T16077	9598-9606	NNP	denotes	Southern
T16076	9595-9597	IN	denotes	by
T16075	9586-9594	VBN	denotes	compared
T16074	9583-9585	IN	denotes	as
T16073	9571-9575	NN	denotes	copy
T16072	9576-9582	NN	denotes	number
T16071	9561-9570	NN	denotes	transgene
T16070	9558-9560	IN	denotes	in
T15888	7461-7468	JJ	denotes	minimal
T15887	7450-7460	VBG	denotes	containing
T15886	7446-7449	NN	denotes	TRE
T15885	7444-7445	DT	denotes	a
T15884	7439-7443	IN	denotes	from
T15883	7431-7433	NN	denotes	E2
T15882	7425-7430	JJ	denotes	human
T15881	7434-7438	NN	denotes	cDNA
T15880	7423-7424	DT	denotes	a
T15879	7415-7422	VB	denotes	express
T15878	7412-7414	TO	denotes	to
T15877	7399-7402	VBD	denotes	was
T15876	7403-7411	VBN	denotes	designed
T15875	7385-7386	HYPH	denotes	-
T15874	7386-7388	NN	denotes	E2
T15873	7382-7385	NN	denotes	TRE
T15872	7389-7398	NN	denotes	transgene
T15871	7378-7381	DT	denotes	The
T15870	7377-7502	sentence	denotes	The TRE-E2 transgene was designed to express a human E2 cDNA from a TRE containing minimal promoter upon stimulation by tTA.
T15869	7376-7377	.	denotes	.
T15868	7375-7376	-RRB-	denotes	)
T15867	7372-7375	NN	denotes	TRE
T15866	7371-7372	-LRB-	denotes	(
T15865	7354-7362	NN	denotes	response
T15864	7339-7353	NN	denotes	transactivator
T15863	7363-7370	NN	denotes	element
T15862	7337-7338	DT	denotes	a
T15861	7330-7336	VBP	denotes	harbor
T15860	7325-7329	WDT	denotes	that
T15859	7315-7324	NNS	denotes	promoters
T15858	7312-7314	IN	denotes	of
T15857	7301-7311	NN	denotes	expression
T15856	7290-7300	VBZ	denotes	stimulates
T15855	7285-7289	WDT	denotes	that
T15854	7264-7277	NN	denotes	transcription
T15853	7278-7284	NN	denotes	factor
T15852	7262-7263	DT	denotes	a
T15851	7260-7262	,	denotes	,
T15850	7259-7260	-RRB-	denotes	)
T15849	7256-7259	NN	denotes	tTA
T15848	7255-7256	-LRB-	denotes	(
T15847	7228-7229	HYPH	denotes	-
T15846	7229-7239	VBN	denotes	controlled
T15845	7216-7228	NN	denotes	tetracycline
T15844	7240-7254	NN	denotes	transactivator
T15843	7212-7215	DT	denotes	the
T15842	7209-7211	IN	denotes	of
T15841	7198-7208	NN	denotes	expression
T15840	7189-7197	JJ	denotes	specific
T15839	7183-7188	NN	denotes	liver
T15838	7180-7182	IN	denotes	of
T15837	7173-7179	NNS	denotes	levels
T15836	7168-7172	JJ	denotes	high
T15835	7158-7159	-RRB-	denotes	]
T15834	7156-7158	CD	denotes	34
T15833	7155-7156	-LRB-	denotes	[
T15832	7160-7167	VBZ	denotes	directs
T15831	7140-7141	HYPH	denotes	-
T15830	7141-7144	NN	denotes	tTA
T15829	7137-7140	NN	denotes	LAP
T15828	7145-7154	NN	denotes	transgene
T15827	7133-7136	DT	denotes	The
T15826	7132-7377	sentence	denotes	The LAP-tTA transgene [34] directs high levels of liver specific expression of the tetracycline-controlled transactivator (tTA), a transcription factor that stimulates expression of promoters that harbor a transactivator response element (TRE).
T15825	7131-7132	.	denotes	.
T15824	7130-7131	-RRB-	denotes	)
T15823	7123-7127	NN	denotes	Fig.
T15822	7128-7130	NN	denotes	3A
T15821	7122-7123	-LRB-	denotes	(
T15820	7108-7109	HYPH	denotes	-
T15819	7109-7111	NN	denotes	E2
T15818	7105-7108	NN	denotes	TRE
T15817	7112-7121	NN	denotes	transgene
T15816	7103-7104	DT	denotes	a
T15815	7099-7102	CC	denotes	and
T15814	7084-7085	HYPH	denotes	-
T15813	7085-7088	NN	denotes	tTA
T15812	7081-7084	NN	denotes	LAP
T15811	7089-7098	NN	denotes	transgene
T15810	7079-7080	DT	denotes	a
T15809	7076-7078	IN	denotes	of
T15808	7066-7075	VBD	denotes	consisted
T15807	7045-7058	JJ	denotes	bi-transgenic
T15806	7059-7065	NN	denotes	system
T15805	7040-7044	DT	denotes	This
T15804	7039-7132	sentence	denotes	This bi-transgenic system consisted of a LAP-tTA transgene and a TRE-E2 transgene (Fig. 3A).
T15803	7038-7039	.	denotes	.
T15802	7019-7027	NN	denotes	knockout
T15801	7016-7018	NN	denotes	E2
T15800	7028-7038	NN	denotes	background
T15799	7012-7015	DT	denotes	the
T15798	7009-7011	IN	denotes	on
T15797	7003-7008	NN	denotes	liver
T15796	7000-7002	IN	denotes	in
T15795	6997-6999	NN	denotes	E2
T15794	6991-6996	JJ	denotes	human
T15793	6983-6990	VB	denotes	express
T15792	6980-6982	TO	denotes	to
T15791	6962-6972	JJ	denotes	transgenic
T15790	6957-6961	NN	denotes	part
T15789	6953-6956	CD	denotes	two
T15788	6973-6979	NN	denotes	system
T15787	6951-6952	DT	denotes	a
T15786	6948-6950	IN	denotes	of
T15785	6935-6938	VBD	denotes	was
T15784	6939-6947	VBN	denotes	composed
T15783	6926-6934	NN	denotes	strategy
T15782	6922-6925	PRP$	denotes	Our
T15781	6921-7039	sentence	denotes	Our strategy was composed of a two part transgenic system to express human E2 in liver on the E2 knockout background.
T15780	6920-6921	.	denotes	.
T15779	6909-6914	NN	denotes	mouse
T15778	6904-6908	NN	denotes	MSUD
T15777	6896-6903	JJ	denotes	classic
T15776	6915-6920	NN	denotes	model
T15775	6892-6895	DT	denotes	the
T15774	6889-6891	IN	denotes	in
T15773	6882-6888	VBZ	denotes	occurs
T15772	6877-6881	WDT	denotes	that
T15771	6867-6876	NN	denotes	lethality
T15770	6858-6866	JJ	denotes	neonatal
T15769	6854-6857	CC	denotes	and
T15768	6849-6853	NN	denotes	BCAA
T15767	6846-6848	IN	denotes	of
T15766	6829-6835	JJ	denotes	severe
T15765	6836-6845	NN	denotes	elevation
T15764	6825-6828	DT	denotes	the
T15763	6818-6824	VB	denotes	rescue
T15762	6815-6817	TO	denotes	to
T15761	6795-6805	JJ	denotes	transgenic
T15760	6806-6814	NN	denotes	strategy
T15759	6793-6794	DT	denotes	a
T15758	6785-6787	PRP	denotes	we
T15757	6783-6785	,	denotes	,
T15756	6779-6783	NN	denotes	MSUD
T15755	6776-6778	IN	denotes	of
T15754	6763-6770	NN	denotes	variant
T15753	6750-6762	JJ	denotes	intermediate
T15752	6771-6775	NN	denotes	form
T15751	6746-6749	DT	denotes	the
T15750	6743-6745	IN	denotes	of
T15749	6730-6736	JJ	denotes	murine
T15748	6737-6742	NN	denotes	model
T15747	6728-6729	DT	denotes	a
T15746	6788-6792	VBD	denotes	used
T15745	6721-6727	VB	denotes	create
T15744	6718-6720	TO	denotes	To
T15743	6717-6921	sentence	denotes	To create a murine model of the intermediate variant form of MSUD, we used a transgenic strategy to rescue the severe elevation of BCAA and neonatal lethality that occurs in the classic MSUD mouse model.
T15742	6713-6717	NN	denotes	MSUD
T15741	6700-6712	JJ	denotes	intermediate
T15740	6693-6699	VBZ	denotes	mimics
T15739	6688-6692	WDT	denotes	that
T15738	6683-6687	NN	denotes	MSUD
T15737	6680-6682	IN	denotes	of
T15736	6667-6674	JJ	denotes	variant
T15735	6675-6679	NN	denotes	form
T15734	6665-6666	DT	denotes	a
T15733	6646-6648	NN	denotes	E2
T15732	6640-6645	JJ	denotes	human
T15731	6649-6658	NN	denotes	transgene
T15730	6638-6639	DT	denotes	a
T15729	6627-6637	VBG	denotes	expressing
T15728	6659-6664	VBP	denotes	model
T15727	6622-6626	NNS	denotes	mice
T15726	6613-6621	NN	denotes	Knockout
T16069	9544-9557	RB	denotes	substantially
T16068	9535-9543	VBD	denotes	differed
T16067	9529-9534	NNS	denotes	lines
T16066	9523-9528	DT	denotes	These
T16065	9522-9650	sentence	denotes	These lines differed substantially in transgene copy number as compared by Southern blot analysis of tail DNA (data not shown).
T16064	9521-9522	.	denotes	.
T16063	9516-9521	NNS	denotes	lines
T16062	9510-9515	DT	denotes	these
T16061	9507-9509	IN	denotes	of
T16060	9505-9506	CD	denotes	9
T16059	9502-9504	IN	denotes	of
T16058	9496-9501	NN	denotes	total
T16057	9494-9495	DT	denotes	a
T16056	9491-9493	IN	denotes	on
T16055	9480-9484	RB	denotes	only
T16054	9477-9479	TO	denotes	to
T16053	9485-9490	VB	denotes	focus
T16052	9474-9476	PRP	denotes	us
T16051	9466-9473	VBD	denotes	allowed
T16050	9456-9465	NNS	denotes	resources
T16049	9448-9455	VBN	denotes	Limited
T16048	9447-9522	sentence	denotes	Limited resources allowed us to only focus on a total of 9 of these lines.
T16047	9446-9447	.	denotes	.
T16046	9438-9446	NNS	denotes	founders
T16045	9432-9437	DT	denotes	these
T16044	9427-9431	IN	denotes	from
T16043	9410-9420	JJ	denotes	transgenic
T16042	9400-9409	JJ	denotes	different
T16041	9421-9426	NNS	denotes	lines
T16040	9397-9399	CD	denotes	15
T16039	9394-9396	IN	denotes	of
T16038	9388-9393	NN	denotes	total
T16037	9386-9387	DT	denotes	a
T16036	9374-9385	VBD	denotes	established
T16035	9371-9373	PRP	denotes	We
T16034	9370-9447	sentence	denotes	We established a total of 15 different transgenic lines from these founders.
T16033	9369-9370	.	denotes	.
T16032	9359-9369	VBD	denotes	segregated
T16031	9354-9358	WDT	denotes	that
T16030	9338-9347	NN	denotes	insertion
T16029	9328-9337	NN	denotes	transgene
T16028	9348-9353	NNS	denotes	sites
T16027	9319-9327	JJ	denotes	multiple
T16026	9307-9314	NNS	denotes	animals
T16025	9303-9306	DT	denotes	the
T16024	9300-9302	IN	denotes	of
T16023	9295-9299	JJ	denotes	many
T16022	9315-9318	VBD	denotes	had
T16021	9290-9294	IN	denotes	that
T16020	9272-9280	NNS	denotes	founders
T16019	9266-9271	DT	denotes	these
T16018	9263-9265	IN	denotes	of
T16017	9281-9289	VBD	denotes	revealed
T16016	9254-9262	NN	denotes	breeding
T16015	9243-9253	JJ	denotes	Subsequent
T16014	9242-9370	sentence	denotes	Subsequent breeding of these founders revealed that many of the animals had multiple transgene insertion sites that segregated.
T16013	9241-9242	.	denotes	.
T16012	9224-9232	JJ	denotes	positive
T16011	9214-9223	NN	denotes	transgene
T16010	9233-9241	NNS	denotes	founders
T16009	9212-9213	CD	denotes	8
T16008	9200-9202	PRP	denotes	we
T16007	9198-9200	,	denotes	,
T16006	9188-9198	NNP	denotes	Cincinnati
T16005	9185-9187	IN	denotes	of
T16004	9174-9184	NNP	denotes	University
T16003	9170-9173	DT	denotes	the
T16002	9167-9169	IN	denotes	at
T16001	9156-9166	NNS	denotes	injections
T16000	9152-9155	DT	denotes	the
T15999	9203-9211	VBD	denotes	obtained
T15998	9147-9151	IN	denotes	From
T15997	9146-9242	sentence	denotes	From the injections at the University of Cincinnati, we obtained 8 transgene positive founders.
T15996	9145-9146	.	denotes	.
T15995	9144-9145	-RRB-	denotes	)
T15994	9143-9144	NN	denotes	D
T15993	9141-9142	CC	denotes	&
T15992	9139-9141	,	denotes	,
T15991	9138-9139	NN	denotes	B
T15990	9136-9138	,	denotes	,
T15989	9129-9134	NNS	denotes	Lines
T15988	9135-9136	NN	denotes	A
T15987	9128-9129	-LRB-	denotes	(
T15986	9111-9121	JJ	denotes	transgenic
T15985	9101-9110	JJ	denotes	different
T15984	9122-9127	NNS	denotes	lines
T15983	9099-9100	CD	denotes	3
T15982	9096-9098	IN	denotes	of
T15981	9085-9095	NN	denotes	generation
T15980	9081-9084	DT	denotes	the
T15979	9078-9080	IN	denotes	to
T15978	9074-9077	VBD	denotes	led
T15977	9069-9073	WDT	denotes	that
T15976	9053-9057	VBD	denotes	were
T15975	9035-9043	JJ	denotes	positive
T15974	9025-9034	NN	denotes	transgene
T15973	9044-9052	NNS	denotes	founders
T15972	9023-9024	CD	denotes	2
T15971	9021-9023	,	denotes	,
T15970	9011-9021	NNP	denotes	Pittsburgh
T15969	9008-9010	IN	denotes	of
T15968	8997-9007	NNP	denotes	University
T15967	8993-8996	DT	denotes	the
T15966	8990-8992	IN	denotes	at
T15965	8979-8989	NNS	denotes	injections
T15964	9058-9068	VBN	denotes	identified
T15963	8974-8978	IN	denotes	From
T15962	8973-9146	sentence	denotes	From injections at the University of Pittsburgh, 2 transgene positive founders were identified that led to the generation of 3 different transgenic lines (Lines A, B, & D).
T15961	8972-8973	.	denotes	.
T15960	8959-8960	HYPH	denotes	-
T12772	6503-6505	IN	denotes	to
T12771	6495-6502	JJ	denotes	similar
T12794	6610-6611	.	denotes	.
T12793	6606-6610	NN	denotes	MSUD
T12792	6598-6605	JJ	denotes	classic
T12791	6584-6591	RB	denotes	closely
T12790	6570-6578	NN	denotes	knockout
T12789	6579-6583	NNS	denotes	mice
T12788	6567-6569	NN	denotes	E2
T12787	6565-6567	,	denotes	,
T12786	6592-6597	VBP	denotes	model
T12785	6561-6565	RB	denotes	Thus
T12784	6560-6611	sentence	denotes	Thus, E2 knockout mice closely model classic MSUD.
T12783	6559-6560	.	denotes	.
T12782	6555-6559	NN	denotes	MSUD
T12781	6552-6554	IN	denotes	of
T12780	6539-6546	JJ	denotes	classic
T12779	6547-6551	NN	denotes	form
T12778	6535-6538	DT	denotes	the
T12777	6530-6534	IN	denotes	with
T12776	6523-6529	NNS	denotes	humans
T12775	6520-6522	IN	denotes	in
T12774	6511-6519	VBN	denotes	observed
T12773	6506-6510	DT	denotes	that
T12770	6484-6494	RB	denotes	remarkably
T12769	6480-6483	VBP	denotes	are
T12768	6469-6479	NNS	denotes	phenotypes
T12767	6463-6468	DT	denotes	These
T12766	6462-6560	sentence	denotes	These phenotypes are remarkably similar to that observed in humans with the classic form of MSUD.
T12765	6461-6462	.	denotes	.
T12764	6452-6461	NN	denotes	lethality
T12763	6443-6451	JJ	denotes	neonatal
T12762	6440-6442	IN	denotes	in
T12761	6422-6432	RB	denotes	ultimately
T12760	6433-6439	VBP	denotes	result
T12759	6399-6408	JJ	denotes	metabolic
T12758	6409-6421	NNS	denotes	derangements
T12757	6393-6398	DT	denotes	These
T12756	6392-6462	sentence	denotes	These metabolic derangements ultimately result in neonatal lethality.
T12755	6391-6392	.	denotes	.
T12754	6386-6391	NN	denotes	urine
T12753	6382-6385	CC	denotes	and
T12752	6376-6381	NN	denotes	blood
T12751	6372-6375	DT	denotes	the
T12750	6369-6371	IN	denotes	in
T12749	6364-6368	NN	denotes	BCAA
T12748	6361-6363	IN	denotes	of
T12747	6354-6360	NNS	denotes	levels
T12746	6345-6353	VBN	denotes	elevated
T12745	6336-6344	RB	denotes	markedly
T12744	6331-6335	VBP	denotes	have
T12743	6327-6330	CC	denotes	and
T12742	6325-6327	,	denotes	,
T12741	6309-6325	NN	denotes	immunoreactivity
T12740	6306-6308	NN	denotes	E2
T12739	6304-6306	,	denotes	,
T12738	6286-6295	JJ	denotes	enzymatic
T10447	401-404	CD	denotes	522
T10367	9-19	NN	denotes	Production
T10368	20-22	IN	denotes	of
T10369	23-25	NN	denotes	E2
T10370	40-44	NNS	denotes	mice
T10371	26-30	NN	denotes	gene
T10372	31-39	NN	denotes	knockout
T10373	44-137	sentence	denotes	To create E2 knockout mice, we used gene targeting in mouse ES cells to disrupt the E2 gene.
T10374	45-47	TO	denotes	To
T10375	48-54	VB	denotes	create
T10376	76-80	VBD	denotes	used
T10377	55-57	NN	denotes	E2
T10378	67-71	NNS	denotes	mice
T10379	58-66	NN	denotes	knockout
T10380	71-73	,	denotes	,
T10381	73-75	PRP	denotes	we
T10382	81-85	NN	denotes	gene
T10383	86-95	NN	denotes	targeting
T10384	96-98	IN	denotes	in
T10385	99-104	NN	denotes	mouse
T10386	108-113	NNS	denotes	cells
T10387	105-107	NN	denotes	ES
T10388	114-116	TO	denotes	to
T10389	117-124	VB	denotes	disrupt
T10390	125-128	DT	denotes	the
T10391	132-136	NN	denotes	gene
T10392	129-131	NN	denotes	E2
T10393	136-137	.	denotes	.
T10394	137-212	sentence	denotes	The overall strategy for disrupting the E2 gene is illustrated in Fig. 1A.
T10395	138-141	DT	denotes	The
T10396	150-158	NN	denotes	strategy
T10397	142-149	JJ	denotes	overall
T10398	189-200	VBN	denotes	illustrated
T10399	159-162	IN	denotes	for
T10400	163-173	VBG	denotes	disrupting
T10401	174-177	DT	denotes	the
T10402	181-185	NN	denotes	gene
T10403	178-180	NN	denotes	E2
T10404	186-188	VBZ	denotes	is
T10405	201-203	IN	denotes	in
T10406	204-208	NN	denotes	Fig.
T10407	209-211	NN	denotes	1A
T10408	211-212	.	denotes	.
T10409	212-397	sentence	denotes	The gene targeting construct was designed to replace a 1.67 kb EcoRV/Smal genomic DNA fragment encompassing part of Exon 4 and all of Exon 5 with the PGKneo selectable marker cassette.
T10410	213-216	DT	denotes	The
T10411	232-241	NN	denotes	construct
T10412	217-221	NN	denotes	gene
T10413	222-231	NN	denotes	targeting
T10414	246-254	VBN	denotes	designed
T10415	242-245	VBD	denotes	was
T10416	255-257	TO	denotes	to
T10417	258-265	VB	denotes	replace
T10418	266-267	DT	denotes	a
T10419	299-307	NN	denotes	fragment
T10420	268-272	CD	denotes	1.67
T10421	273-275	NN	denotes	kb
T10422	276-281	NN	denotes	EcoRV
T10423	282-286	NN	denotes	Smal
T10424	281-282	HYPH	denotes	/
T10425	287-294	JJ	denotes	genomic
T10426	295-298	NN	denotes	DNA
T10427	308-320	VBG	denotes	encompassing
T10428	321-325	NN	denotes	part
T10429	326-328	IN	denotes	of
T10430	329-333	NN	denotes	Exon
T10431	334-335	CD	denotes	4
T10432	336-339	CC	denotes	and
T10433	340-343	DT	denotes	all
T10434	344-346	IN	denotes	of
T10435	347-351	NN	denotes	Exon
T10436	352-353	CD	denotes	5
T10437	354-358	IN	denotes	with
T10438	359-362	DT	denotes	the
T10439	388-396	NN	denotes	cassette
T10440	363-369	NN	denotes	PGKneo
T10441	370-380	JJ	denotes	selectable
T10442	381-387	NN	denotes	marker
T10443	396-397	.	denotes	.
T10444	397-589	sentence	denotes	Of 522 ES cell clones screened for targeting by Southern blot analysis, 29 (5%) displayed the predicted restriction fragment length polymorphisms indicative of gene targeting at the E2 locus.
T10445	398-400	IN	denotes	Of
T10446	478-487	VBD	denotes	displayed
T10448	413-419	NNS	denotes	clones
T10449	405-407	NN	denotes	ES
T10450	408-412	NN	denotes	cell
T10451	420-428	VBN	denotes	screened
T10452	429-432	IN	denotes	for
T10453	433-442	NN	denotes	targeting
T10454	443-445	IN	denotes	by
T10455	446-454	NNP	denotes	Southern
T10456	455-459	NN	denotes	blot
T10457	460-468	NN	denotes	analysis
T10458	468-470	,	denotes	,
T10459	470-472	CD	denotes	29
T10460	473-474	-LRB-	denotes	(
T10461	475-476	NN	denotes	%
T10462	474-475	CD	denotes	5
T10463	476-477	-RRB-	denotes	)
T10464	488-491	DT	denotes	the
T10465	530-543	NNS	denotes	polymorphisms
T10466	492-501	VBN	denotes	predicted
T10467	502-513	NN	denotes	restriction
T10468	523-529	NN	denotes	length
T10469	514-522	NN	denotes	fragment
T10470	544-554	JJ	denotes	indicative
T10471	555-557	IN	denotes	of
T10472	558-562	NN	denotes	gene
T10473	563-572	VBG	denotes	targeting
T10474	573-575	IN	denotes	at
T10475	576-579	DT	denotes	the
T10476	583-588	NN	denotes	locus
T10477	580-582	NN	denotes	E2
T10478	588-589	.	denotes	.
T10479	589-811	sentence	denotes	As illustrated in Fig. 1A and 1B, an E2 Exon 6 specific probe, which is external to the targeting construct, hybridizes to only a ~16 kb BglI restriction fragment from the wild type allele in the parental R1 ES cell line.
T10480	590-592	IN	denotes	As
T10481	593-604	VBN	denotes	illustrated
T10482	699-709	VBZ	denotes	hybridizes
T10483	605-607	IN	denotes	in
T10484	608-612	NN	denotes	Fig.
T10485	613-615	NN	denotes	1A
T10486	616-619	CC	denotes	and
T10487	620-622	NN	denotes	1B
T10488	622-624	,	denotes	,
T10489	624-626	DT	denotes	an
T10490	646-651	NN	denotes	probe
T10491	627-629	NN	denotes	E2
T10492	630-634	NN	denotes	Exon
T10493	635-636	CD	denotes	6
T10494	637-645	JJ	denotes	specific
T10495	651-653	,	denotes	,
T10496	653-658	WDT	denotes	which
T10497	659-661	VBZ	denotes	is
T10498	662-670	JJ	denotes	external
T10499	671-673	IN	denotes	to
T10500	674-677	DT	denotes	the
T10501	688-697	NN	denotes	construct
T10502	678-687	NN	denotes	targeting
T10503	697-699	,	denotes	,
T10504	710-712	IN	denotes	to
T10505	713-717	RB	denotes	only
T10506	744-752	NN	denotes	fragment
T10507	718-719	DT	denotes	a
T10508	720-721	SYM	denotes	~
T10509	721-723	CD	denotes	16
T10510	724-726	NN	denotes	kb
T10511	727-731	NN	denotes	BglI
T10512	732-743	NN	denotes	restriction
T10513	753-757	IN	denotes	from
T10514	758-761	DT	denotes	the
T10515	772-778	NN	denotes	allele
T10516	762-766	JJ	denotes	wild
T10517	767-771	NN	denotes	type
T10518	779-781	IN	denotes	in
T10519	782-785	DT	denotes	the
T10520	806-810	NN	denotes	line
T10521	786-794	JJ	denotes	parental
T10522	795-797	NN	denotes	R1
T10523	798-800	NN	denotes	ES
T10524	801-805	NN	denotes	cell
T10525	810-811	.	denotes	.
T10526	811-909	sentence	denotes	In correctly targeted ES cells, this probe also hybridizes to a ~11 kb BglI restriction fragment.
T10527	812-814	IN	denotes	In
T10528	860-870	VBZ	denotes	hybridizes
T10529	815-824	RB	denotes	correctly
T10530	825-833	VBN	denotes	targeted
T10531	837-842	NNS	denotes	cells
T10532	834-836	NN	denotes	ES
T10533	842-844	,	denotes	,
T10534	844-848	DT	denotes	this
T10535	849-854	NN	denotes	probe
T10536	855-859	RB	denotes	also
T10537	871-873	IN	denotes	to
T10538	874-875	DT	denotes	a
T10539	900-908	NN	denotes	fragment
T10540	876-877	SYM	denotes	~
T10541	877-879	CD	denotes	11
T10542	880-882	NN	denotes	kb
T10543	883-887	NN	denotes	BglI
T10544	888-899	NN	denotes	restriction
T10545	908-909	.	denotes	.
T10546	909-1006	sentence	denotes	Targeting was confirmed with several additional restriction enzymes and probes (data not shown).
T10547	910-919	NN	denotes	Targeting
T10548	924-933	VBN	denotes	confirmed
T10549	920-923	VBD	denotes	was
T10550	934-938	IN	denotes	with
T10551	939-946	JJ	denotes	several
T10552	970-977	NNS	denotes	enzymes
T10553	947-957	JJ	denotes	additional
T10554	958-969	NN	denotes	restriction
T10555	978-981	CC	denotes	and
T10556	982-988	NNS	denotes	probes
T10557	989-990	-LRB-	denotes	(
T10558	999-1004	VBN	denotes	shown
T10559	990-994	NNS	denotes	data
T10560	995-998	RB	denotes	not
T10561	1004-1005	-RRB-	denotes	)
T10562	1005-1006	.	denotes	.
T10563	1006-1096	sentence	denotes	Correctly targeted ES cells were microinjected into blastocysts to produce chimeric mice.
T10564	1007-1016	RB	denotes	Correctly
T10565	1017-1025	VBN	denotes	targeted
T10566	1029-1034	NNS	denotes	cells
T10567	1026-1028	NN	denotes	ES
T10568	1040-1053	VBN	denotes	microinjected
T10569	1035-1039	VBD	denotes	were
T10570	1054-1058	IN	denotes	into
T10571	1059-1070	NNS	denotes	blastocysts
T10572	1071-1073	TO	denotes	to
T10573	1074-1081	VB	denotes	produce
T10574	1082-1090	JJ	denotes	chimeric
T10575	1091-1095	NNS	denotes	mice
T10576	1095-1096	.	denotes	.
T10577	1096-1134	sentence	denotes	Chimerics were bred to C57BL/6J mice.
T10578	1097-1106	NNS	denotes	Chimerics
T10579	1112-1116	VBN	denotes	bred
T10580	1107-1111	VBD	denotes	were
T10581	1117-1119	IN	denotes	to
T10582	1120-1125	NN	denotes	C57BL
T10583	1126-1128	NN	denotes	6J
T10584	1125-1126	HYPH	denotes	/
T10585	1129-1133	NNS	denotes	mice
T10586	1133-1134	.	denotes	.
T10587	1134-1300	sentence	denotes	Following germline transmission of the targeted allele, heterozygous mice were interbred to produce wild type (+/+), heterozygous (+/-) and homozygous (-/-) animals.
T10588	1135-1144	VBG	denotes	Following
T10589	1214-1223	VBN	denotes	interbred
T10590	1145-1153	NN	denotes	germline
T10591	1154-1166	NN	denotes	transmission
T10592	1167-1169	IN	denotes	of
T10593	1170-1173	DT	denotes	the
T10594	1183-1189	NN	denotes	allele
T10595	1174-1182	VBN	denotes	targeted
T10596	1189-1191	,	denotes	,
T10597	1191-1203	JJ	denotes	heterozygous
T10598	1204-1208	NNS	denotes	mice
T10599	1209-1213	VBD	denotes	were
T10600	1224-1226	TO	denotes	to
T10601	1227-1234	VB	denotes	produce
T10602	1235-1239	JJ	denotes	wild
T10603	1240-1244	NN	denotes	type
T10604	1292-1299	NNS	denotes	animals
T10605	1245-1246	-LRB-	denotes	(
T10606	1248-1249	SYM	denotes	+
T10607	1246-1247	SYM	denotes	+
T10608	1247-1248	HYPH	denotes	/
T10609	1249-1250	-RRB-	denotes	)
T10610	1250-1252	,	denotes	,
T10611	1252-1264	JJ	denotes	heterozygous
T10612	1265-1266	-LRB-	denotes	(
T10613	1268-1269	SYM	denotes	-
T10614	1266-1267	SYM	denotes	+
T10615	1267-1268	HYPH	denotes	/
T10616	1269-1270	-RRB-	denotes	)
T10617	1271-1274	CC	denotes	and
T10618	1275-1285	JJ	denotes	homozygous
T10619	1286-1287	-LRB-	denotes	(
T10620	1289-1290	SYM	denotes	-
T10621	1287-1288	SYM	denotes	-
T10622	1288-1289	HYPH	denotes	/
T10623	1290-1291	-RRB-	denotes	)
T10624	1299-1300	.	denotes	.
T10625	1300-1347	sentence	denotes	Mice were genotyped by Southern blot analysis.
T10626	1301-1305	NNS	denotes	Mice
T10627	1311-1320	VBN	denotes	genotyped
T10628	1306-1310	VBD	denotes	were
T10629	1321-1323	IN	denotes	by
T10630	1324-1332	NNP	denotes	Southern
T10631	1333-1337	NN	denotes	blot
T10632	1338-1346	NN	denotes	analysis
T10633	1346-1347	.	denotes	.
T10634	1347-1530	sentence	denotes	The Exon 6 specific probe hybridized to only a ~16 kb BglI restriction fragment in +/+ mice, a ~11 kb BglI fragment in -/- mice, and to both of these fragments in +/- mice (Fig. 1B).
T10635	1348-1351	DT	denotes	The
T10636	1368-1373	NN	denotes	probe
T10637	1352-1356	NN	denotes	Exon
T10638	1359-1367	JJ	denotes	specific
T10639	1357-1358	CD	denotes	6
T10640	1374-1384	VBN	denotes	hybridized
T10641	1385-1387	IN	denotes	to
T10642	1388-1392	RB	denotes	only
T10643	1419-1427	NN	denotes	fragment
T10644	1393-1394	DT	denotes	a
T10645	1395-1396	SYM	denotes	~
T10646	1396-1398	CD	denotes	16
T10647	1399-1401	NN	denotes	kb
T10648	1402-1406	NN	denotes	BglI
T10649	1407-1418	NN	denotes	restriction
T10650	1428-1430	IN	denotes	in
T10651	1431-1432	SYM	denotes	+
T10652	1433-1434	SYM	denotes	+
T10653	1432-1433	HYPH	denotes	/
T10654	1435-1439	NNS	denotes	mice
T10655	1439-1441	,	denotes	,
T10656	1441-1442	DT	denotes	a
T10657	1455-1463	NN	denotes	fragment
T10658	1443-1444	SYM	denotes	~
T10659	1444-1446	CD	denotes	11
T10660	1447-1449	NN	denotes	kb
T10661	1450-1454	NN	denotes	BglI
T10662	1464-1466	IN	denotes	in
T10663	1467-1468	SYM	denotes	-
T10664	1469-1470	SYM	denotes	-
T10665	1468-1469	HYPH	denotes	/
T10666	1471-1475	NNS	denotes	mice
T10667	1475-1477	,	denotes	,
T10668	1477-1480	CC	denotes	and
T10669	1481-1483	IN	denotes	to
T10670	1484-1488	DT	denotes	both
T10671	1489-1491	IN	denotes	of
T10672	1492-1497	DT	denotes	these
T10673	1498-1507	NNS	denotes	fragments
T10674	1508-1510	IN	denotes	in
T10675	1511-1512	SYM	denotes	+
T10676	1513-1514	SYM	denotes	-
T10677	1512-1513	HYPH	denotes	/
T10678	1515-1519	NNS	denotes	mice
T10679	1520-1521	-LRB-	denotes	(
T10680	1526-1528	NN	denotes	1B
T10681	1521-1525	NN	denotes	Fig.
T10682	1528-1529	-RRB-	denotes	)
T10683	1529-1530	.	denotes	.
T10684	1530-1603	sentence	denotes	Mice homozygous for the E2 mutation were born at the expected frequency.
T10685	1531-1535	NNS	denotes	Mice
T10686	1572-1576	VBN	denotes	born
T10687	1536-1546	JJ	denotes	homozygous
T10688	1547-1550	IN	denotes	for
T10689	1551-1554	DT	denotes	the
T10690	1558-1566	NN	denotes	mutation
T10691	1555-1557	NN	denotes	E2
T10692	1567-1571	VBD	denotes	were
T10693	1577-1579	IN	denotes	at
T10694	1580-1583	DT	denotes	the
T10695	1593-1602	NN	denotes	frequency
T10696	1584-1592	VBN	denotes	expected
T10697	1602-1603	.	denotes	.
T10698	1603-1751	sentence	denotes	Genotype analysis of pups derived from +/- by +/- matings revealed that +/+, +/-, and -/- mice were present at nearly the expected 1:2:1 frequency.
T10699	1604-1612	NN	denotes	Genotype
T10700	1613-1621	NN	denotes	analysis
T10701	1662-1670	VBD	denotes	revealed
T10702	1622-1624	IN	denotes	of
T10703	1625-1629	NNS	denotes	pups
T10704	1630-1637	VBN	denotes	derived
T10705	1638-1642	IN	denotes	from
T10706	1643-1644	SYM	denotes	+
T10707	1645-1646	SYM	denotes	-
T10708	1644-1645	HYPH	denotes	/
T10709	1654-1661	NNS	denotes	matings
T10710	1647-1649	IN	denotes	by
T10711	1650-1651	SYM	denotes	+
T10712	1652-1653	SYM	denotes	-
T10713	1651-1652	HYPH	denotes	/
T10714	1671-1675	IN	denotes	that
T10715	1699-1703	VBD	denotes	were
T10716	1676-1677	SYM	denotes	+
T10717	1678-1679	SYM	denotes	+
T10718	1677-1678	HYPH	denotes	/
T10719	1694-1698	NNS	denotes	mice
T10720	1679-1681	,	denotes	,
T10721	1681-1682	SYM	denotes	+
T10722	1683-1684	SYM	denotes	-
T10723	1682-1683	HYPH	denotes	/
T10724	1684-1686	,	denotes	,
T10725	1686-1689	CC	denotes	and
T10726	1690-1691	SYM	denotes	-
T10727	1692-1693	SYM	denotes	-
T10728	1691-1692	HYPH	denotes	/
T10729	1704-1711	JJ	denotes	present
T10730	1712-1714	IN	denotes	at
T10731	1715-1721	RB	denotes	nearly
T10732	1741-1750	NN	denotes	frequency
T10733	1722-1725	DT	denotes	the
T10734	1726-1734	VBN	denotes	expected
T10735	1735-1736	CD	denotes	1
T10736	1739-1740	CD	denotes	1
T10737	1736-1737	SYM	denotes	:
T10738	1737-1738	CD	denotes	2
T10739	1738-1739	SYM	denotes	:
T10740	1750-1751	.	denotes	.
T10741	1751-1849	sentence	denotes	Of the initial 60 animals genotyped, 19 (32%) were +/+, 27 (45%) were +/-, and 14 (23%) were -/-.
T10742	1752-1754	IN	denotes	Of
T10743	1798-1802	VBD	denotes	were
T10744	1755-1758	DT	denotes	the
T10745	1770-1777	NNS	denotes	animals
T10746	1759-1766	JJ	denotes	initial
T10747	1767-1769	CD	denotes	60
T10748	1778-1787	VBN	denotes	genotyped
T10749	1787-1789	,	denotes	,
T10750	1789-1791	CD	denotes	19
T10751	1792-1793	-LRB-	denotes	(
T10752	1795-1796	NN	denotes	%
T10753	1793-1795	CD	denotes	32
T10754	1796-1797	-RRB-	denotes	)
T10755	1803-1804	SYM	denotes	+
T10756	1805-1806	SYM	denotes	+
T10757	1804-1805	HYPH	denotes	/
T10758	1806-1808	,	denotes	,
T10759	1808-1810	CD	denotes	27
T10760	1817-1821	VBD	denotes	were
T10761	1811-1812	-LRB-	denotes	(
T10762	1814-1815	NN	denotes	%
T10763	1812-1814	CD	denotes	45
T10764	1815-1816	-RRB-	denotes	)
T10765	1822-1823	SYM	denotes	+
T10766	1824-1825	SYM	denotes	-
T10767	1823-1824	HYPH	denotes	/
T10768	1825-1827	,	denotes	,
T10769	1827-1830	CC	denotes	and
T10770	1831-1833	CD	denotes	14
T10771	1840-1844	VBD	denotes	were
T10772	1834-1835	-LRB-	denotes	(
T10773	1837-1838	NN	denotes	%
T10774	1835-1837	CD	denotes	23
T10775	1838-1839	-RRB-	denotes	)
T10776	1845-1846	SYM	denotes	-
T10777	1847-1848	SYM	denotes	-
T10778	1846-1847	HYPH	denotes	/
T10779	1848-1849	.	denotes	.
T10780	1849-1917	sentence	denotes	Thus, the E2 gene was dispensable for normal embryonic development.
T10781	1850-1854	RB	denotes	Thus
T10782	1868-1871	VBD	denotes	was
T10783	1854-1856	,	denotes	,
T10784	1856-1859	DT	denotes	the
T10785	1863-1867	NN	denotes	gene
T10786	1860-1862	NN	denotes	E2
T10787	1872-1883	JJ	denotes	dispensable
T10788	1884-1887	IN	denotes	for
T10789	1888-1894	JJ	denotes	normal
T10790	1905-1916	NN	denotes	development
T10791	1895-1904	JJ	denotes	embryonic
T10792	1916-1917	.	denotes	.
T10793	1917-1996	sentence	denotes	However, as expected, nearly all homozygous mice died in the perinatal period.
T10794	1918-1925	RB	denotes	However
T10795	1967-1971	VBD	denotes	died
T10796	1925-1927	,	denotes	,
T10797	1927-1929	IN	denotes	as
T10798	1930-1938	VBN	denotes	expected
T10799	1938-1940	,	denotes	,
T10800	1940-1946	RB	denotes	nearly
T10801	1962-1966	NNS	denotes	mice
T10802	1947-1950	DT	denotes	all
T10803	1951-1961	JJ	denotes	homozygous
T10804	1972-1974	IN	denotes	in
T10805	1975-1978	DT	denotes	the
T10806	1989-1995	NN	denotes	period
T10807	1979-1988	JJ	denotes	perinatal
T10808	1995-1996	.	denotes	.
T10809	1996-2147	sentence	denotes	Immediately following birth, homozygous pups were indistinguishable from their +/+ and +/- littermates; they were vigorous, active and able to suckle.
T10810	1997-2008	RB	denotes	Immediately
T10811	2009-2018	VBG	denotes	following
T10812	2042-2046	VBD	denotes	were
T10813	2019-2024	NN	denotes	birth
T10814	2024-2026	,	denotes	,
T10815	2026-2036	JJ	denotes	homozygous
T10816	2037-2041	NNS	denotes	pups
T10817	2106-2110	VBD	denotes	were
T10818	2047-2064	JJ	denotes	indistinguishable
T10819	2065-2069	IN	denotes	from
T10820	2070-2075	PRP$	denotes	their
T10821	2088-2099	NNS	denotes	littermates
T10822	2076-2077	SYM	denotes	+
T10823	2078-2079	SYM	denotes	+
T10824	2077-2078	HYPH	denotes	/
T10825	2080-2083	CC	denotes	and
T10826	2084-2085	SYM	denotes	+
T10827	2086-2087	SYM	denotes	-
T10828	2085-2086	HYPH	denotes	/
T10829	2099-2100	:	denotes	;
T10830	2101-2105	PRP	denotes	they
T10831	2111-2119	JJ	denotes	vigorous
T10832	2119-2121	,	denotes	,
T10833	2121-2127	JJ	denotes	active
T10834	2128-2131	CC	denotes	and
T10835	2132-2136	JJ	denotes	able
T10836	2137-2139	TO	denotes	to
T10837	2140-2146	VB	denotes	suckle
T10838	2146-2147	.	denotes	.
T10839	2147-2323	sentence	denotes	By mid to late day on postnatal day one, most -/- pups became moribund and were readily identifiable as they were lethargic, pale, and exhibited gasping respiratory movements.
T10840	2148-2150	IN	denotes	By
T10841	2203-2209	VBD	denotes	became
T10842	2151-2154	JJ	denotes	mid
T10843	2163-2166	NN	denotes	day
T10844	2155-2157	IN	denotes	to
T10845	2158-2162	JJ	denotes	late
T10846	2167-2169	IN	denotes	on
T10847	2170-2179	JJ	denotes	postnatal
T10848	2180-2183	NN	denotes	day
T10849	2184-2187	CD	denotes	one
T10850	2187-2189	,	denotes	,
T10851	2189-2193	RBS	denotes	most
T10852	2198-2202	NNS	denotes	pups
T10853	2194-2195	SYM	denotes	-
T10854	2196-2197	SYM	denotes	-
T10855	2195-2196	HYPH	denotes	/
T10856	2210-2218	JJ	denotes	moribund
T10857	2219-2222	CC	denotes	and
T10858	2223-2227	VBD	denotes	were
T10859	2228-2235	RB	denotes	readily
T10860	2236-2248	JJ	denotes	identifiable
T10861	2249-2251	IN	denotes	as
T10862	2257-2261	VBD	denotes	were
T10863	2252-2256	PRP	denotes	they
T10864	2262-2271	JJ	denotes	lethargic
T10865	2273-2277	JJ	denotes	pale
T10866	2271-2273	,	denotes	,
T10867	2277-2279	,	denotes	,
T10868	2279-2282	CC	denotes	and
T10869	2283-2292	VBD	denotes	exhibited
T10870	2293-2300	VBG	denotes	gasping
T10871	2313-2322	NNS	denotes	movements
T10872	2301-2312	JJ	denotes	respiratory
T10873	2322-2323	.	denotes	.
T10874	2323-2384	sentence	denotes	With few exceptions, -/- pups died within 72 hours of birth.
T10875	2324-2328	IN	denotes	With
T10876	2354-2358	VBD	denotes	died
T10877	2329-2332	JJ	denotes	few
T10878	2333-2343	NNS	denotes	exceptions
T10879	2343-2345	,	denotes	,
T10880	2345-2346	SYM	denotes	-
T10881	2347-2348	SYM	denotes	-
T10882	2346-2347	HYPH	denotes	/
T10883	2349-2353	NNS	denotes	pups
T10884	2359-2365	IN	denotes	within
T10885	2366-2368	CD	denotes	72
T10886	2369-2374	NNS	denotes	hours
T10887	2375-2377	IN	denotes	of
T10888	2378-2383	NN	denotes	birth
T10889	2383-2384	.	denotes	.
T10890	2384-2453	sentence	denotes	We have observed one rare -/- pup that survived to postnatal day 13.
T10891	2385-2387	PRP	denotes	We
T10892	2393-2401	VBN	denotes	observed
T10893	2388-2392	VBP	denotes	have
T10894	2402-2405	CD	denotes	one
T10895	2415-2418	NN	denotes	pup
T10896	2406-2410	JJ	denotes	rare
T10897	2411-2412	SYM	denotes	-
T10898	2413-2414	SYM	denotes	-
T10899	2412-2413	HYPH	denotes	/
T10900	2419-2423	WDT	denotes	that
T10901	2424-2432	VBD	denotes	survived
T10902	2433-2435	IN	denotes	to
T10903	2436-2445	JJ	denotes	postnatal
T10904	2446-2449	NN	denotes	day
T10905	2450-2452	CD	denotes	13
T10906	2452-2453	.	denotes	.
T10907	2453-2515	sentence	denotes	The reason for the prolonged survival of this pup is unknown.
T10908	2454-2457	DT	denotes	The
T10909	2458-2464	NN	denotes	reason
T10910	2504-2506	VBZ	denotes	is
T10911	2465-2468	IN	denotes	for
T10912	2469-2472	DT	denotes	the
T10913	2483-2491	NN	denotes	survival
T10914	2473-2482	VBN	denotes	prolonged
T10915	2492-2494	IN	denotes	of
T10916	2495-2499	DT	denotes	this
T10917	2500-2503	NN	denotes	pup
T10918	2507-2514	JJ	denotes	unknown
T10919	2514-2515	.	denotes	.
T12206	2517-2519	NN	denotes	E2
T12207	2520-2529	JJ	denotes	deficient
T12208	2530-2534	NNS	denotes	mice
T12209	2546-2551	VBP	denotes	model
T12210	2535-2545	RB	denotes	accurately
T12211	2552-2559	JJ	denotes	classic
T12212	2560-2564	NN	denotes	MSUD
T12213	2564-2758	sentence	denotes	To demonstrate that the gene targeting event created a true E2 null allele, we determined BCKDH activity in liver homogenates of postnatal day 1 mouse pups derived from +/- by +/- mating pairs.
T12214	2565-2567	TO	denotes	To
T12215	2568-2579	VB	denotes	demonstrate
T12216	2644-2654	VBD	denotes	determined
T12217	2580-2584	IN	denotes	that
T12218	2610-2617	VBD	denotes	created
T12219	2585-2588	DT	denotes	the
T12220	2604-2609	NN	denotes	event
T12221	2589-2593	NN	denotes	gene
T12222	2594-2603	NN	denotes	targeting
T12223	2618-2619	DT	denotes	a
T12224	2633-2639	NN	denotes	allele
T12225	2620-2624	JJ	denotes	true
T12226	2625-2627	NN	denotes	E2
T12227	2628-2632	JJ	denotes	null
T12228	2639-2641	,	denotes	,
T12229	2641-2643	PRP	denotes	we
T12230	2655-2660	NN	denotes	BCKDH
T12231	2661-2669	NN	denotes	activity
T12232	2670-2672	IN	denotes	in
T12233	2673-2678	NN	denotes	liver
T12234	2679-2690	NNS	denotes	homogenates
T12235	2691-2693	IN	denotes	of
T12236	2694-2703	JJ	denotes	postnatal
T12237	2704-2707	NN	denotes	day
T12238	2716-2720	NNS	denotes	pups
T12239	2708-2709	CD	denotes	1
T12247	2738-2740	IN	denotes	by
T12248	2741-2742	SYM	denotes	+
T12249	2743-2744	SYM	denotes	-
T12250	2742-2743	HYPH	denotes	/
T12251	2745-2751	NN	denotes	mating
T12252	2757-2758	.	denotes	.
T12253	2758-2836	sentence	denotes	As shown in Figure 2A, the BCKDH activity in +/+ mice was readily detectable.
T12254	2759-2761	IN	denotes	As
T12255	2762-2767	VBN	denotes	shown
T12256	2813-2816	VBD	denotes	was
T12257	2768-2770	IN	denotes	in
T12258	2771-2777	NN	denotes	Figure
T12259	2778-2780	NN	denotes	2A
T12260	2780-2782	,	denotes	,
T12261	2782-2785	DT	denotes	the
T12262	2792-2800	NN	denotes	activity
T12263	2786-2791	NN	denotes	BCKDH
T12264	2801-2803	IN	denotes	in
T12265	2804-2805	SYM	denotes	+
T12266	2806-2807	SYM	denotes	+
T12267	2805-2806	HYPH	denotes	/
T12268	2808-2812	NNS	denotes	mice
T12269	2817-2824	RB	denotes	readily
T12270	2825-2835	JJ	denotes	detectable
T12271	2835-2836	.	denotes	.
T12272	2836-2914	sentence	denotes	In marked contrast, BCKDH activity was completely absent in -/- mouse livers.
T12273	2837-2839	IN	denotes	In
T12274	2872-2875	VBD	denotes	was
T12275	2840-2846	JJ	denotes	marked
T12276	2847-2855	NN	denotes	contrast
T12277	2855-2857	,	denotes	,
T12278	2857-2862	NN	denotes	BCKDH
T12279	2863-2871	NN	denotes	activity
T12280	2876-2886	RB	denotes	completely
T12281	2887-2893	JJ	denotes	absent
T12282	2894-2896	IN	denotes	in
T12283	2897-2898	SYM	denotes	-
T12284	2899-2900	SYM	denotes	-
T12285	2898-2899	HYPH	denotes	/
T12286	2907-2913	NNS	denotes	livers
T12287	2901-2906	NN	denotes	mouse
T12288	2913-2914	.	denotes	.
T12289	2914-3027	sentence	denotes	As expected, homogenates from +/- mice had approximately half the activity of their +/+ littermates (Figure 2A).
T12290	2915-2917	IN	denotes	As
T12291	2918-2926	VBN	denotes	expected
T12292	2954-2957	VBD	denotes	had
T12293	2926-2928	,	denotes	,
T12294	2928-2939	NNS	denotes	homogenates
T12295	2940-2944	IN	denotes	from
T12296	2945-2946	SYM	denotes	+
T12297	2947-2948	SYM	denotes	-
T12298	2946-2947	HYPH	denotes	/
T12299	2949-2953	NNS	denotes	mice
T12300	2958-2971	RB	denotes	approximately
T12301	2972-2976	PDT	denotes	half
T12302	2981-2989	NN	denotes	activity
T12303	2977-2980	DT	denotes	the
T12304	2990-2992	IN	denotes	of
T12305	2993-2998	PRP$	denotes	their
T12306	3003-3014	NNS	denotes	littermates
T12307	2999-3000	SYM	denotes	+
T12308	3001-3002	SYM	denotes	+
T12309	3000-3001	HYPH	denotes	/
T12310	3015-3016	-LRB-	denotes	(
T12311	3023-3025	NN	denotes	2A
T12312	3016-3022	NN	denotes	Figure
T12313	3025-3026	-RRB-	denotes	)
T12314	3026-3027	.	denotes	.
T12315	3027-3115	sentence	denotes	The results from -/- mice are similar to that observed in humans with classic MSUD [1].
T12316	3028-3031	DT	denotes	The
T12317	3032-3039	NNS	denotes	results
T12318	3054-3057	VBP	denotes	are
T12319	3040-3044	IN	denotes	from
T12320	3045-3046	SYM	denotes	-
T12321	3047-3048	SYM	denotes	-
T12322	3046-3047	HYPH	denotes	/
T12323	3049-3053	NNS	denotes	mice
T12324	3058-3065	JJ	denotes	similar
T12325	3066-3068	IN	denotes	to
T12326	3069-3073	DT	denotes	that
T12327	3074-3082	VBN	denotes	observed
T12328	3083-3085	IN	denotes	in
T12329	3086-3092	NNS	denotes	humans
T12330	3093-3097	IN	denotes	with
T12331	3098-3105	JJ	denotes	classic
T12332	3106-3110	NN	denotes	MSUD
T12333	3111-3112	-LRB-	denotes	[
T12334	3112-3113	CD	denotes	1
T12335	3113-3114	-RRB-	denotes	]
T12336	3114-3115	.	denotes	.
T12337	3115-3116	sentence	denotes
T12346	4004-4007	VBD	denotes	was
T12347	4013-4015	TO	denotes	to
T12348	4016-4023	VB	denotes	examine
T12349	4024-4026	NN	denotes	E2
T12350	4027-4034	NN	denotes	protein
T12351	4035-4037	IN	denotes	in
T12352	4038-4041	DT	denotes	the
T12353	4042-4046	NNS	denotes	mice
T12354	4046-4047	.	denotes	.
T12355	4047-4163	sentence	denotes	As shown in Figure 1C and 1D, immunoreactive E2 protein was abundant in liver and embryonic fibroblasts of+/+ mice.
T12356	4048-4050	IN	denotes	As
T12357	4051-4056	VBN	denotes	shown
T12358	4104-4107	VBD	denotes	was
T12359	4057-4059	IN	denotes	in
T12360	4060-4066	NN	denotes	Figure
T12361	4067-4069	NN	denotes	1C
T12362	4070-4073	CC	denotes	and
T12363	4074-4076	NN	denotes	1D
T12364	4076-4078	,	denotes	,
T12365	4078-4092	JJ	denotes	immunoreactive
T12366	4096-4103	NN	denotes	protein
T12367	4093-4095	NN	denotes	E2
T12368	4108-4116	JJ	denotes	abundant
T12369	4117-4119	IN	denotes	in
T12370	4120-4125	NN	denotes	liver
T12371	4126-4129	CC	denotes	and
T12372	4130-4139	JJ	denotes	embryonic
T12373	4140-4151	NNS	denotes	fibroblasts
T12374	4152-4154	IN	denotes	of
T12375	4154-4155	SYM	denotes	+
T12376	4156-4157	SYM	denotes	+
T12377	4155-4156	HYPH	denotes	/
T12378	4158-4162	NNS	denotes	mice
T12379	4162-4163	.	denotes	.
T12380	4163-4255	sentence	denotes	In marked contrast, immunoreactive E2 protein was absent in these same tissues of -/- mice.
T12381	4164-4166	IN	denotes	In
T12382	4210-4213	VBD	denotes	was
T12383	4167-4173	JJ	denotes	marked
T12384	4174-4182	NN	denotes	contrast
T12385	4182-4184	,	denotes	,
T12386	4184-4198	JJ	denotes	immunoreactive
T12387	4202-4209	NN	denotes	protein
T12388	4199-4201	NN	denotes	E2
T12389	4214-4220	JJ	denotes	absent
T12390	4221-4223	IN	denotes	in
T12391	4224-4229	DT	denotes	these
T12392	4235-4242	NNS	denotes	tissues
T12393	4230-4234	JJ	denotes	same
T12394	4243-4245	IN	denotes	of
T12395	4246-4247	SYM	denotes	-
T12396	4248-4249	SYM	denotes	-
T12397	4247-4248	HYPH	denotes	/
T12398	4250-4254	NNS	denotes	mice
T12399	4254-4255	.	denotes	.
T12400	4255-4454	sentence	denotes	Homozygous E2 knockout mice had a nearly 3-fold increase in blood (Figure 2B) and urine (data not shown) levels of BCAA (sum of leucine, isoleucine, and valine) as compared to their +/+ littermates.
T12401	4256-4266	JJ	denotes	Homozygous
T12402	4270-4278	NN	denotes	knockout
T12403	4267-4269	NN	denotes	E2
T12404	4279-4283	NNS	denotes	mice
T12405	4284-4287	VBD	denotes	had
T12406	4288-4289	DT	denotes	a
T12407	4304-4312	NN	denotes	increase
T12408	4290-4296	RB	denotes	nearly
T12409	4297-4298	CD	denotes	3
T12410	4299-4303	JJ	denotes	fold
T12411	4298-4299	HYPH	denotes	-
T12412	4313-4315	IN	denotes	in
T12413	4316-4321	NN	denotes	blood
T12414	4361-4367	NNS	denotes	levels
T12415	4322-4323	-LRB-	denotes	(
T12416	4330-4332	NN	denotes	2B
T12417	4323-4329	NN	denotes	Figure
T12418	4332-4333	-RRB-	denotes	)
T12419	4334-4337	CC	denotes	and
T12420	4338-4343	NN	denotes	urine
T12421	4344-4345	-LRB-	denotes	(
T12422	4354-4359	VBN	denotes	shown
T12423	4345-4349	NNS	denotes	data
T12424	4350-4353	RB	denotes	not
T12425	4359-4360	-RRB-	denotes	)
T12426	4368-4370	IN	denotes	of
T12427	4371-4375	NN	denotes	BCAA
T12428	4376-4377	-LRB-	denotes	(
T12429	4377-4380	NN	denotes	sum
T12430	4381-4383	IN	denotes	of
T12431	4384-4391	NN	denotes	leucine
T12432	4391-4393	,	denotes	,
T12433	4393-4403	NN	denotes	isoleucine
T12434	4403-4405	,	denotes	,
T12435	4405-4408	CC	denotes	and
T12436	4409-4415	NN	denotes	valine
T12437	4415-4416	-RRB-	denotes	)
T12438	4417-4419	IN	denotes	as
T12439	4420-4428	VBN	denotes	compared
T12440	4429-4431	IN	denotes	to
T12441	4432-4437	PRP$	denotes	their
T12442	4442-4453	NNS	denotes	littermates
T12443	4438-4439	SYM	denotes	+
T12444	4440-4441	SYM	denotes	+
T12445	4439-4440	HYPH	denotes	/
T12446	4453-4454	.	denotes	.
T12447	4454-4630	sentence	denotes	Because amino acids were analyzed by tandem mass spectrometry, the sum of BCAA shown in Figure 2B also may include alloisoleucine that may have been produced in -/- MSUD mice.
T12448	4455-4462	IN	denotes	Because
T12449	4480-4488	VBN	denotes	analyzed
T12450	4463-4468	NN	denotes	amino
T12451	4469-4474	NNS	denotes	acids
T12452	4475-4479	VBD	denotes	were
T12453	4562-4569	VB	denotes	include
T12454	4489-4491	IN	denotes	by
T12455	4492-4498	NN	denotes	tandem
T12456	4504-4516	NN	denotes	spectrometry
T12457	4499-4503	NN	denotes	mass
T12458	4516-4518	,	denotes	,
T12459	4518-4521	DT	denotes	the
T12460	4522-4525	NN	denotes	sum
T12461	4526-4528	IN	denotes	of
T12462	4529-4533	NN	denotes	BCAA
T12463	4534-4539	VBN	denotes	shown
T12464	4540-4542	IN	denotes	in
T12465	4543-4549	NN	denotes	Figure
T12466	4550-4552	NN	denotes	2B
T12467	4553-4557	RB	denotes	also
T12468	4558-4561	MD	denotes	may
T12469	4570-4584	NN	denotes	alloisoleucine
T12470	4585-4589	WDT	denotes	that
T12471	4604-4612	VBN	denotes	produced
T12472	4590-4593	MD	denotes	may
T12473	4594-4598	VB	denotes	have
T12474	4599-4603	VBN	denotes	been
T12475	4613-4615	IN	denotes	in
T12476	4616-4617	SYM	denotes	-
T12477	4618-4619	SYM	denotes	-
T12478	4617-4618	HYPH	denotes	/
T12479	4625-4629	NNS	denotes	mice
T12480	4620-4624	NN	denotes	MSUD
T12481	4629-4630	.	denotes	.
T12482	4630-4725	sentence	denotes	The metabolism of BCAA is linked with the synthesis of alanine, glutamate, and glutamine [33].
T12483	4631-4634	DT	denotes	The
T12484	4635-4645	NN	denotes	metabolism
T12485	4657-4663	VBN	denotes	linked
T12486	4646-4648	IN	denotes	of
T12487	4649-4653	NN	denotes	BCAA
T12488	4654-4656	VBZ	denotes	is
T12489	4664-4668	IN	denotes	with
T12490	4669-4672	DT	denotes	the
T12491	4673-4682	NN	denotes	synthesis
T12492	4683-4685	IN	denotes	of
T12493	4686-4693	NN	denotes	alanine
T12494	4693-4695	,	denotes	,
T12495	4695-4704	NN	denotes	glutamate
T12496	4704-4706	,	denotes	,
T12497	4706-4709	CC	denotes	and
T12498	4710-4719	NN	denotes	glutamine
T12499	4720-4721	-LRB-	denotes	[
T12500	4721-4723	CD	denotes	33
T12501	4723-4724	-RRB-	denotes	]
T12502	4724-4725	.	denotes	.
T12503	4725-4918	sentence	denotes	Because of impaired metabolism of BCAA in MSUD mice, and to further characterize the abnormal biochemistry in this model, we analyzed the blood levels of the alanine, glutamate, and glutamine.
T12504	4726-4733	IN	denotes	Because
T12505	4851-4859	VBD	denotes	analyzed
T12506	4734-4736	IN	denotes	of
T12507	4737-4745	JJ	denotes	impaired
T12508	4746-4756	NN	denotes	metabolism
T12509	4757-4759	IN	denotes	of
T12510	4760-4764	NN	denotes	BCAA
T12511	4765-4767	IN	denotes	in
T12512	4768-4772	NN	denotes	MSUD
T12513	4773-4777	NNS	denotes	mice
T12514	4777-4779	,	denotes	,
T12515	4779-4782	CC	denotes	and
T12516	4783-4785	TO	denotes	to
T12517	4794-4806	VB	denotes	characterize
T12518	4786-4793	RB	denotes	further
T12519	4807-4810	DT	denotes	the
T12520	4820-4832	NN	denotes	biochemistry
T12521	4811-4819	JJ	denotes	abnormal
T12522	4833-4835	IN	denotes	in
T12523	4836-4840	DT	denotes	this
T12524	4841-4846	NN	denotes	model
T12525	4846-4848	,	denotes	,
T12526	4848-4850	PRP	denotes	we
T12527	4860-4863	DT	denotes	the
T12528	4870-4876	NNS	denotes	levels
T12529	4864-4869	NN	denotes	blood
T12530	4877-4879	IN	denotes	of
T12531	4880-4883	DT	denotes	the
T12532	4884-4891	NN	denotes	alanine
T12533	4891-4893	,	denotes	,
T12534	4893-4902	NN	denotes	glutamate
T12535	4902-4904	,	denotes	,
T12536	4904-4907	CC	denotes	and
T12537	4908-4917	NN	denotes	glutamine
T12538	4917-4918	.	denotes	.
T12539	4918-5050	sentence	denotes	As shown in Table 1, the levels of all three amino acids in homozygous mice were markedly lower than the levels in +/+ or +/- mice.
T12540	4919-4921	IN	denotes	As
T12541	4922-4927	VBN	denotes	shown
T12542	4995-4999	VBD	denotes	were
T12543	4928-4930	IN	denotes	in
T12544	4931-4936	NN	denotes	Table
T12545	4937-4938	CD	denotes	1
T12546	4938-4940	,	denotes	,
T12547	4940-4943	DT	denotes	the
T12548	4944-4950	NNS	denotes	levels
T12549	4951-4953	IN	denotes	of
T12550	4954-4957	DT	denotes	all
T12551	4970-4975	NNS	denotes	acids
T12552	4958-4963	CD	denotes	three
T12553	4964-4969	NN	denotes	amino
T12554	4976-4978	IN	denotes	in
T12555	4979-4989	JJ	denotes	homozygous
T12556	4990-4994	NNS	denotes	mice
T12557	5000-5008	RB	denotes	markedly
T12558	5009-5014	JJR	denotes	lower
T12559	5015-5019	IN	denotes	than
T12560	5020-5023	DT	denotes	the
T12561	5024-5030	NNS	denotes	levels
T12562	5031-5033	IN	denotes	in
T12563	5034-5035	SYM	denotes	+
T12564	5036-5037	SYM	denotes	+
T12565	5035-5036	HYPH	denotes	/
T12566	5045-5049	NNS	denotes	mice
T12567	5038-5040	CC	denotes	or
T12568	5041-5042	SYM	denotes	+
T12569	5043-5044	SYM	denotes	-
T12570	5042-5043	HYPH	denotes	/
T12571	5049-5050	.	denotes	.
T12572	5050-5146	sentence	denotes	The levels of these amino acids in the +/- mice were comparable to those in +/+ mice (Table 1).
T12573	5051-5054	DT	denotes	The
T12574	5055-5061	NNS	denotes	levels
T12575	5099-5103	VBD	denotes	were
T12576	5062-5064	IN	denotes	of
T12577	5065-5070	DT	denotes	these
T12578	5077-5082	NNS	denotes	acids
T12579	5071-5076	NN	denotes	amino
T12580	5083-5085	IN	denotes	in
T12581	5086-5089	DT	denotes	the
T12582	5094-5098	NNS	denotes	mice
T12583	5090-5091	SYM	denotes	+
T12584	5092-5093	SYM	denotes	-
T12585	5091-5092	HYPH	denotes	/
T12586	5104-5114	JJ	denotes	comparable
T12587	5115-5117	IN	denotes	to
T12588	5118-5123	DT	denotes	those
T12589	5124-5126	IN	denotes	in
T12590	5127-5128	SYM	denotes	+
T12591	5129-5130	SYM	denotes	+
T12592	5128-5129	HYPH	denotes	/
T12593	5131-5135	NNS	denotes	mice
T12594	5136-5137	-LRB-	denotes	(
T12595	5137-5142	NN	denotes	Table
T12596	5143-5144	CD	denotes	1
T12597	5144-5145	-RRB-	denotes	)
T12598	5145-5146	.	denotes	.
T12599	5146-5452	sentence	denotes	Because of the abnormal decrease in the blood alanine level and marked rise in blood BCAA levels that are characteristic of MSUD, a recent report on MSUD patients has suggested that the ratio of BCAA/alanine provides a more sensitive measure of the abnormal biochemistry of MSUD than BCAA level alone [8].
T12600	5147-5154	IN	denotes	Because
T12601	5314-5323	VBN	denotes	suggested
T12602	5155-5157	IN	denotes	of
T12603	5158-5161	DT	denotes	the
T12604	5171-5179	NN	denotes	decrease
T12605	5162-5170	JJ	denotes	abnormal
T12606	5180-5182	IN	denotes	in
T12607	5183-5186	DT	denotes	the
T12608	5201-5206	NN	denotes	level
T12609	5187-5192	NN	denotes	blood
T12610	5193-5200	NN	denotes	alanine
T12611	5207-5210	CC	denotes	and
T12612	5211-5217	JJ	denotes	marked
T12613	5218-5222	NN	denotes	rise
T12614	5223-5225	IN	denotes	in
T12615	5226-5231	NN	denotes	blood
T12616	5237-5243	NNS	denotes	levels
T12617	5232-5236	NN	denotes	BCAA
T12618	5244-5248	WDT	denotes	that
T12619	5249-5252	VBP	denotes	are
T12620	5253-5267	JJ	denotes	characteristic
T12621	5268-5270	IN	denotes	of
T12622	5271-5275	NN	denotes	MSUD
T12623	5275-5277	,	denotes	,
T12624	5277-5278	DT	denotes	a
T12625	5286-5292	NN	denotes	report
T12626	5279-5285	JJ	denotes	recent
T12627	5293-5295	IN	denotes	on
T12628	5296-5300	NN	denotes	MSUD
T12629	5301-5309	NNS	denotes	patients
T12630	5310-5313	VBZ	denotes	has
T12631	5324-5328	IN	denotes	that
T12632	5355-5363	VBZ	denotes	provides
T12633	5329-5332	DT	denotes	the
T12634	5333-5338	NN	denotes	ratio
T12635	5339-5341	IN	denotes	of
T12636	5342-5346	NN	denotes	BCAA
T12637	5347-5354	NN	denotes	alanine
T12638	5346-5347	HYPH	denotes	/
T12639	5364-5365	DT	denotes	a
T12640	5381-5388	NN	denotes	measure
T12641	5366-5370	RBR	denotes	more
T12642	5371-5380	JJ	denotes	sensitive
T12643	5389-5391	IN	denotes	of
T12644	5392-5395	DT	denotes	the
T12645	5405-5417	NN	denotes	biochemistry
T12646	5396-5404	JJ	denotes	abnormal
T12647	5418-5420	IN	denotes	of
T12648	5421-5425	NN	denotes	MSUD
T12649	5426-5430	IN	denotes	than
T12650	5431-5435	NN	denotes	BCAA
T12651	5436-5441	NN	denotes	level
T12652	5442-5447	RB	denotes	alone
T12653	5448-5449	-LRB-	denotes	[
T12654	5449-5450	CD	denotes	8
T12655	5450-5451	-RRB-	denotes	]
T12656	5451-5452	.	denotes	.
T12657	5452-5527	sentence	denotes	Therefore, we also expressed the amino acid results as BCAA/alanine ratio.
T12658	5453-5462	RB	denotes	Therefore
T12659	5472-5481	VBD	denotes	expressed
T12660	5462-5464	,	denotes	,
T12661	5464-5466	PRP	denotes	we
T12662	5467-5471	RB	denotes	also
T12701	5616-5617	SYM	denotes	-
T12702	5615-5616	HYPH	denotes	/
T12703	5629-5630	.	denotes	.
T12704	5630-5737	sentence	denotes	These blood amino acid results are consistent with the concentrations seen in patients with MSUD [1,8,15].
T12705	5631-5636	DT	denotes	These
T12706	5654-5661	NNS	denotes	results
T12707	5637-5642	NN	denotes	blood
T12708	5643-5648	NN	denotes	amino
T12709	5649-5653	NN	denotes	acid
T12710	5662-5665	VBP	denotes	are
T12711	5666-5676	JJ	denotes	consistent
T12712	5677-5681	IN	denotes	with
T12713	5682-5685	DT	denotes	the
T12714	5686-5700	NNS	denotes	concentrations
T12715	5701-5705	VBN	denotes	seen
T12716	5706-5708	IN	denotes	in
T12717	5709-5717	NNS	denotes	patients
T12718	5718-5722	IN	denotes	with
T12719	5723-5727	NN	denotes	MSUD
T12720	5728-5729	-LRB-	denotes	[
T12721	5733-5735	CD	denotes	15
T12722	5729-5730	CD	denotes	1
T12723	5730-5731	,	denotes	,
T12724	5731-5732	CD	denotes	8
T12725	5732-5733	,	denotes	,
T12726	5735-5736	-RRB-	denotes	]
T12727	5736-5737	.	denotes	.
T17015	15918-15924	JJ	denotes	useful
T22807	3126-3137	JJ	denotes	Biochemical
T22808	3138-3154	NN	denotes	characterization
T22809	3155-3157	IN	denotes	of
T22810	3158-3161	DT	denotes	the
T22811	3182-3187	NN	denotes	model
T22812	3162-3169	JJ	denotes	classic
T22813	3170-3174	NN	denotes	MSUD
T22814	3175-3181	JJ	denotes	murine
T22815	3187-3188	.	denotes	.
T22816	3188-3314	sentence	denotes	A, BCKDH enzyme activity in liver of newborn wild type control (+/+), heterozygous (+/-), and homozygous (-/-) knockout mice.
T22817	3189-3190	LS	denotes	A
T22818	3205-3213	NN	denotes	activity
T22819	3190-3192	,	denotes	,
T22820	3192-3197	NN	denotes	BCKDH
T22821	3198-3204	NN	denotes	enzyme
T22822	3214-3216	IN	denotes	in
T22823	3217-3222	NN	denotes	liver
T22824	3223-3225	IN	denotes	of
T22825	3226-3233	JJ	denotes	newborn
T22826	3309-3313	NNS	denotes	mice
T22827	3234-3238	JJ	denotes	wild
T22828	3239-3243	NN	denotes	type
T22829	3244-3251	NN	denotes	control
T22830	3300-3308	NN	denotes	knockout
T22831	3252-3253	-LRB-	denotes	(
T22832	3255-3256	SYM	denotes	+
T22833	3253-3254	SYM	denotes	+
T22834	3254-3255	HYPH	denotes	/
T22835	3256-3257	-RRB-	denotes	)
T22836	3257-3259	,	denotes	,
T22837	3259-3271	JJ	denotes	heterozygous
T22838	3272-3273	-LRB-	denotes	(
T22839	3275-3276	SYM	denotes	-
T22840	3273-3274	SYM	denotes	+
T22841	3274-3275	HYPH	denotes	/
T22842	3276-3277	-RRB-	denotes	)
T22843	3277-3279	,	denotes	,
T22844	3279-3282	CC	denotes	and
T22845	3283-3293	JJ	denotes	homozygous
T22846	3294-3295	-LRB-	denotes	(
T22847	3297-3298	SYM	denotes	-
T22848	3295-3296	SYM	denotes	-
T22849	3296-3297	HYPH	denotes	/
T22850	3298-3299	-RRB-	denotes	)
T22851	3313-3314	.	denotes	.
T22852	3314-3437	sentence	denotes	Enzyme activity was significantly reduced in +/- liver compared to +/+, and was below the level of detection in -/- liver.
T22853	3315-3321	NN	denotes	Enzyme
T22854	3322-3330	NN	denotes	activity
T22855	3331-3334	VBD	denotes	was
T22856	3335-3348	RB	denotes	significantly
T22857	3349-3356	JJ	denotes	reduced
T22858	3357-3359	IN	denotes	in
T22859	3360-3361	SYM	denotes	+
T22860	3362-3363	SYM	denotes	-
T22861	3361-3362	HYPH	denotes	/
T22862	3364-3369	NN	denotes	liver
T22863	3370-3378	VBN	denotes	compared
T22864	3379-3381	IN	denotes	to
T22865	3382-3383	SYM	denotes	+
T22866	3384-3385	SYM	denotes	+
T22867	3383-3384	HYPH	denotes	/
T22868	3385-3387	,	denotes	,
T22869	3387-3390	CC	denotes	and
T22870	3391-3394	VBD	denotes	was
T22871	3395-3400	IN	denotes	below
T22872	3401-3404	DT	denotes	the
T22873	3405-3410	NN	denotes	level
T22874	3411-3413	IN	denotes	of
T22875	3414-3423	NN	denotes	detection
T22876	3424-3426	IN	denotes	in
T22877	3427-3428	SYM	denotes	-
T22878	3429-3430	SYM	denotes	-
T22879	3428-3429	HYPH	denotes	/
T22880	3431-3436	NN	denotes	liver
T22881	3436-3437	.	denotes	.
T22882	3437-3484	sentence	denotes	B, Total BCAA concentrations in blood of mice.
T22883	3438-3439	LS	denotes	B
T22884	3452-3466	NNS	denotes	concentrations
T22885	3439-3441	,	denotes	,
T22886	3441-3446	JJ	denotes	Total
T22887	3447-3451	NN	denotes	BCAA
T22888	3467-3469	IN	denotes	in
T22889	3470-3475	NN	denotes	blood
T22890	3476-3478	IN	denotes	of
T22891	3479-3483	NNS	denotes	mice
T22892	3483-3484	.	denotes	.
T22893	3484-3549	sentence	denotes	Total BCAA represent the sum of leucine, isoleucine, and valine.
T22894	3485-3490	JJ	denotes	Total
T22895	3491-3495	NN	denotes	BCAA
T22896	3496-3505	VBP	denotes	represent
T22897	3506-3509	DT	denotes	the
T22898	3510-3513	NN	denotes	sum
T22899	3514-3516	IN	denotes	of
T22900	3517-3524	NN	denotes	leucine
T22901	3524-3526	,	denotes	,
T22902	3526-3536	NN	denotes	isoleucine
T22903	3536-3538	,	denotes	,
T22904	3538-3541	CC	denotes	and
T22905	3542-3548	NN	denotes	valine
T22906	3548-3549	.	denotes	.
T22907	3549-3651	sentence	denotes	Total BCAA concentrations in blood from -/- mice were significantly elevated compared to +/+ and +/-.
T22908	3550-3555	JJ	denotes	Total
T22909	3561-3575	NNS	denotes	concentrations
T22910	3556-3560	NN	denotes	BCAA
T22911	3599-3603	VBD	denotes	were
T22912	3576-3578	IN	denotes	in
T22913	3579-3584	NN	denotes	blood
T22914	3585-3589	IN	denotes	from
T22915	3590-3591	SYM	denotes	-
T22916	3592-3593	SYM	denotes	-
T22917	3591-3592	HYPH	denotes	/
T22918	3594-3598	NNS	denotes	mice
T22919	3604-3617	RB	denotes	significantly
T22920	3618-3626	JJ	denotes	elevated
T22921	3627-3635	VBN	denotes	compared
T22922	3636-3638	IN	denotes	to
T22923	3639-3640	SYM	denotes	+
T22924	3641-3642	SYM	denotes	+
T22925	3640-3641	HYPH	denotes	/
T22926	3643-3646	CC	denotes	and
T22927	3647-3648	SYM	denotes	+
T22928	3649-3650	SYM	denotes	-
T22929	3648-3649	HYPH	denotes	/
T22930	3650-3651	.	denotes	.
T22931	3651-3703	sentence	denotes	C, Ratio of total BCAA to alanine in blood of mice.
T22932	3652-3653	LS	denotes	C
T22933	3655-3660	NN	denotes	Ratio
T22934	3653-3655	,	denotes	,
T22935	3661-3663	IN	denotes	of
T22936	3664-3669	JJ	denotes	total
T22937	3670-3674	NN	denotes	BCAA
T22938	3675-3677	IN	denotes	to
T22939	3678-3685	NN	denotes	alanine
T22940	3686-3688	IN	denotes	in
T22941	3689-3694	NN	denotes	blood
T22942	3695-3697	IN	denotes	of
T22943	3698-3702	NNS	denotes	mice
T22944	3702-3703	.	denotes	.
T22945	3703-3783	sentence	denotes	This ratio was significantly elevated in -/- mice compared to +/+ and +/- mice.
T22946	3704-3708	DT	denotes	This
T22947	3709-3714	NN	denotes	ratio
T22948	3715-3718	VBD	denotes	was
T22949	3719-3732	RB	denotes	significantly
T22950	3733-3741	JJ	denotes	elevated
T22951	3742-3744	IN	denotes	in
T22952	3745-3746	SYM	denotes	-
T22953	3747-3748	SYM	denotes	-
T22954	3746-3747	HYPH	denotes	/
T22955	3749-3753	NNS	denotes	mice
T22956	3754-3762	VBN	denotes	compared
T22957	3763-3765	IN	denotes	to
T22958	3766-3767	SYM	denotes	+
T22959	3768-3769	SYM	denotes	+
T22960	3767-3768	HYPH	denotes	/
T22961	3778-3782	NNS	denotes	mice
T22962	3770-3773	CC	denotes	and
T22963	3774-3775	SYM	denotes	+
T22964	3776-3777	SYM	denotes	-
T22965	3775-3776	HYPH	denotes	/
T22966	3782-3783	.	denotes	.
T22967	3783-3846	sentence	denotes	The numbers on the bars indicates the number of mice analyzed.
T22968	3784-3787	DT	denotes	The
T22969	3788-3795	NNS	denotes	numbers
T22970	3808-3817	VBZ	denotes	indicates
T22971	3796-3798	IN	denotes	on
T22972	3799-3802	DT	denotes	the
T22973	3803-3807	NNS	denotes	bars
T22974	3818-3821	DT	denotes	the
T22975	3822-3828	NN	denotes	number
T22976	3829-3831	IN	denotes	of
T22977	3832-3836	NNS	denotes	mice
T22978	3837-3845	VBN	denotes	analyzed
T22979	3845-3846	.	denotes	.
T22980	3846-3953	sentence	denotes	, Significantly different from +/+ (P < 0.001); *, significantly different from +/+ and +/- (P < 0.001).
T22981	3847-3848	SYM	denotes	*
T22982	3896-3898	SYM	denotes	**
T22983	3848-3850	,	denotes	,
T22984	3850-3863	RB	denotes	Significantly
T22985	3864-3873	JJ	denotes	different
T22986	3874-3878	IN	denotes	from
T22987	3879-3880	SYM	denotes	+
T22988	3881-3882	SYM	denotes	+
T22989	3880-3881	HYPH	denotes	/
T22990	3883-3884	-LRB-	denotes	(
T22991	3888-3893	CD	denotes	0.001
T22992	3884-3885	NN	denotes	P
T22993	3886-3887	SYM	denotes	<
T22994	3893-3894	-RRB-	denotes	)
T22995	3894-3895	:	denotes	;
T22996	3898-3900	,	denotes	,
T22997	3900-3913	RB	denotes	significantly
T22998	3914-3923	JJ	denotes	different
T22999	3924-3928	IN	denotes	from
T23000	3929-3930	SYM	denotes	+
T23001	3931-3932	SYM	denotes	+
T23002	3930-3931	HYPH	denotes	/
T23003	3933-3936	CC	denotes	and
T23004	3937-3938	SYM	denotes	+
T23005	3939-3940	SYM	denotes	-
T23006	3938-3939	HYPH	denotes	/
T23007	3941-3942	-LRB-	denotes	(
T23008	3946-3951	CD	denotes	0.001
T23009	3942-3943	NN	denotes	P
T23010	3944-3945	SYM	denotes	<
T23011	3951-3952	-RRB-	denotes	)
T23012	3952-3953	.	denotes	.
T23928	8787-8788	.	denotes	.
T24488	11183-11185	IN	denotes	of
T24487	11174-11182	NN	denotes	knockout
T24486	11170-11173	IN	denotes	for
T24485	11157-11159	,	denotes	,
T24484	11153-11157	FW	denotes	i.e.
T24483	11159-11169	JJ	denotes	homozygous
T24687	12085-12090	NNS	denotes	cages
T24686	12079-12084	PRP$	denotes	their
T24685	12076-12078	IN	denotes	in
T24684	12071-12075	JJ	denotes	dead
T24683	12065-12070	VBN	denotes	found
T24682	12060-12064	VBD	denotes	were
T24681	12057-12059	CC	denotes	or
T24774	5747-5754	NN	denotes	Summary
T24775	5755-5757	IN	denotes	of
T24776	5758-5768	JJ	denotes	additional
T24777	5786-5792	NNS	denotes	levels
T24778	5769-5774	NN	denotes	blood
T24779	5775-5780	NN	denotes	amino
T24780	5781-5785	NN	denotes	acid
T24781	5793-5795	IN	denotes	in
T24782	5796-5799	DT	denotes	the
T24783	5813-5818	NN	denotes	model
T24784	5800-5807	JJ	denotes	classic
T24785	5808-5812	NN	denotes	MSUD
T24786	5819-5822	CC	denotes	and
T24787	5823-5830	NN	denotes	control
T24788	5831-5842	NNS	denotes	littermates
T24789	5843-5845	IN	denotes	as
T24790	5846-5856	VBN	denotes	determined
T24791	5857-5859	IN	denotes	by
T24792	5860-5866	JJ	denotes	tandem
T24793	5872-5884	NN	denotes	spectrometry
T24794	5867-5871	NN	denotes	mass
T24795	5884-5885	.	denotes	.
T24796	5885-5933	sentence	denotes	All samples were collected on the day of birth.
T24797	5886-5889	DT	denotes	All
T24798	5890-5897	NNS	denotes	samples
T24799	5903-5912	VBN	denotes	collected
T24800	5898-5902	VBD	denotes	were
T24801	5913-5915	IN	denotes	on
T24802	5916-5919	DT	denotes	the
T24803	5920-5923	NN	denotes	day
T24804	5924-5926	IN	denotes	of
T24805	5927-5932	NN	denotes	birth
T24806	5932-5933	.	denotes	.
T24807	5933-5962	sentence	denotes	All values are mean +/- SEM.
T24808	5934-5937	DT	denotes	All
T24809	5938-5944	NNS	denotes	values
T24810	5945-5948	VBP	denotes	are
T24811	5949-5953	NN	denotes	mean
T24812	5954-5955	SYM	denotes	+
T24813	5955-5956	HYPH	denotes	/
T24814	5956-5957	SYM	denotes	-
T24815	5958-5961	NN	denotes	SEM
T24816	5961-5962	.	denotes	.
T12690	5583-5587	IN	denotes	than
T12691	5590-5594	RB	denotes	fold
T12692	5589-5590	HYPH	denotes	-
T12693	5595-5601	JJR	denotes	higher
T12694	5602-5606	IN	denotes	than
T12695	5607-5608	SYM	denotes	+
T12696	5609-5610	SYM	denotes	+
T12697	5608-5609	HYPH	denotes	/
T12240	2710-2715	NN	denotes	mouse
T12241	2721-2728	VBN	denotes	derived
T12242	2729-2733	IN	denotes	from
T12243	2734-2735	SYM	denotes	+
T12244	2736-2737	SYM	denotes	-
T12245	2735-2736	HYPH	denotes	/
T12246	2752-2757	NNS	denotes	pairs
T12338	3954-4047	sentence	denotes	Immunohistochemistry with an E2 specific antibody was used to examine E2 protein in the mice.
T12339	3954-3974	NN	denotes	Immunohistochemistry
T12340	4008-4012	VBN	denotes	used
T12341	3975-3979	IN	denotes	with
T12342	3980-3982	DT	denotes	an
T12343	3995-4003	NN	denotes	antibody
T12344	3983-3985	NN	denotes	E2
T12345	3986-3994	JJ	denotes	specific
T12698	5618-5629	NNS	denotes	littermates
T12663	5482-5485	DT	denotes	the
T12664	5497-5504	NNS	denotes	results
T12665	5486-5491	NN	denotes	amino
T12666	5492-5496	NN	denotes	acid
T12667	5505-5507	IN	denotes	as
T12668	5508-5512	NN	denotes	BCAA
T12669	5513-5520	NN	denotes	alanine
T12670	5512-5513	HYPH	denotes	/
T12671	5521-5526	NN	denotes	ratio
T12672	5526-5527	.	denotes	.
T12673	5527-5630	sentence	denotes	As shown in Figure 2C, this ratio in -/- pups was more than 6-fold higher than +/+ or +/- littermates.
T12674	5528-5530	IN	denotes	As
T12675	5531-5536	VBN	denotes	shown
T12676	5574-5577	VBD	denotes	was
T12677	5537-5539	IN	denotes	in
T12678	5540-5546	NN	denotes	Figure
T12679	5547-5549	NN	denotes	2C
T12680	5549-5551	,	denotes	,
T12681	5551-5555	DT	denotes	this
T12682	5556-5561	NN	denotes	ratio
T12683	5562-5564	IN	denotes	in
T12684	5565-5566	SYM	denotes	-
T12685	5567-5568	SYM	denotes	-
T12686	5566-5567	HYPH	denotes	/
T12687	5569-5573	NNS	denotes	pups
T12688	5578-5582	RBR	denotes	more
T12689	5588-5589	CD	denotes	6
T12699	5611-5613	CC	denotes	or
T12700	5614-5615	SYM	denotes	+
T12737	6296-6304	NN	denotes	activity
T12736	6280-6285	NN	denotes	BCKDH
T12735	6261-6269	NN	denotes	knockout
T12734	6270-6274	NNS	denotes	mice
T12733	6258-6260	NN	denotes	E2
T12732	6256-6258	,	denotes	,
T12731	6249-6256	NN	denotes	summary
T12730	6275-6279	VBP	denotes	lack
T12729	6246-6248	IN	denotes	In
T12728	5737-6392	sentence	denotes	Table 1 Summary of additional blood amino acid levels in the classic MSUD model and control littermates as determined by tandem mass spectrometry. All samples were collected on the day of birth. All values are mean +/- SEM. MSUD Genotype Alanine Glutamate Glutamine (μmole/L) +/+ 196.1 ± 23.1 (6) 229.0 ± 12.0 (6) 76.8 ± 3.0 (6) +/- 167.6 ± 9.9 (13) 251.0 ± 12.8 (13) 72.3 ± 1.6 (13) -/- 94.6 ± 11.6* (5) 107.8 ± 4.8* (5) 50.4 ± 2.9* (5) *: Significantly different from +/+ and +/- (P < 0.001). In summary, E2 knockout mice lack BCKDH enzymatic activity, E2 immunoreactivity, and have markedly elevated levels of BCAA in the blood and urine.
T15938	8848-8851	DT	denotes	the
T15937	8845-8847	IN	denotes	by
T15936	8841-8844	VBN	denotes	zed
T15935	8840-8841	NNS	denotes	i
T15934	8840-8875	sentence	denotes	ized by the Bujard laboratory [34].
T15900	8797-8801	NNS	denotes	mice
T15899	8792-8793	HYPH	denotes	-
T15898	8793-8796	NN	denotes	tTA
T15897	8789-8792	NN	denotes	LAP
T15896	8789-8802	sentence	denotes	LAP-tTA mice
T15895	7502-7503	sentence	denotes
T15894	7501-7502	.	denotes	.
T15893	7498-7501	NN	denotes	tTA
T15892	7495-7497	IN	denotes	by
T15891	7483-7494	NN	denotes	stimulation
T15890	7478-7482	IN	denotes	upon
T15889	7469-7477	NN	denotes	promoter
T15911	8813-8814	NN	denotes	u
T15910	8812-8813	VB	denotes	o
T15909	8812-8818	sentence	denotes	ously
T15908	8811-8812	JJ	denotes	i
T15907	8810-8811	NN	denotes	v
T15906	8807-8810	NN	denotes	pre
T15905	8807-8812	sentence	denotes	previ
T15904	8804-8806	VB	denotes	re
T15903	8802-8803	VBD	denotes	w
T15902	8803-8804	VBN	denotes	e
T15901	8802-8807	sentence	denotes	were
T15917	8821-8822	NN	denotes	d
T15916	8818-8821	NN	denotes	pro
T15915	8818-8825	sentence	denotes	produce
T15914	8816-8817	JJ	denotes	y
T15913	8815-8816	JJ	denotes	l
T15912	8814-8815	NNS	denotes	s
T15959	8960-8962	NN	denotes	E2
T15958	8956-8959	NN	denotes	TRE
T15957	8963-8972	NN	denotes	transgene
T15956	8952-8955	DT	denotes	the
T15955	8943-8951	VBD	denotes	harbored
T15954	8938-8942	WDT	denotes	that
T15953	8933-8937	NNS	denotes	mice
T15952	8922-8932	JJ	denotes	transgenic
T15951	8914-8921	VB	denotes	produce
T15950	8911-8913	TO	denotes	to
T15949	8902-8905	VBD	denotes	was
T15948	8906-8910	VBN	denotes	used
T15947	8887-8901	NN	denotes	microinjection
T15946	8876-8886	JJ	denotes	Pronuclear
T15945	8875-8973	sentence	denotes	Pronuclear microinjection was used to produce transgenic mice that harbored the TRE-E2 transgene.
T15944	8874-8875	.	denotes	.
T15943	8873-8874	-RRB-	denotes	]
T15942	8871-8873	CD	denotes	34
T15941	8870-8871	-LRB-	denotes	[
T15940	8852-8858	NNP	denotes	Bujard
T15939	8859-8869	NN	denotes	laboratory
T15933	8838-8839	VB	denotes	e
T15932	8837-8838	TO	denotes	t
T15931	8836-8837	DT	denotes	c
T15930	8835-8836	VBN	denotes	a
T15929	8834-8835	NN	denotes	r
T15928	8839-8840	VBN	denotes	r
T15927	8833-8834	DT	denotes	a
T15926	8833-8840	sentence	denotes	aracter
T15925	8832-8833	DT	denotes	h
T15924	8831-8832	NN	denotes	c
T15923	8827-8830	CC	denotes	and
T15922	8825-8826	NN	denotes	d
T15921	8825-8833	sentence	denotes	d and ch
T15920	8824-8825	NNS	denotes	e
T15919	8823-8824	JJ	denotes	c
T15918	8822-8823	JJ	denotes	u
T16321	10851-10860	VBG	denotes	surviving
T16320	10835-10841	VBN	denotes	tested
T16319	10842-10850	VBD	denotes	produced
T16318	10823-10828	JJ	denotes	other
T16317	10829-10834	NNS	denotes	lines
T16316	10819-10822	DT	denotes	All
T16315	10818-10923	sentence	denotes	All other lines tested produced surviving rescue animals at a frequency between 1 and 5% of weaned pups.
T16314	10817-10818	.	denotes	.
T16285	10652-10654	TO	denotes	to
T16284	10629-10633	NN	denotes	525A
T16283	10634-10643	NN	denotes	transgene
T16282	10625-10628	DT	denotes	the
T16281	10623-10625	,	denotes	,
T16280	10644-10651	VBZ	denotes	appears
T16279	10619-10623	RB	denotes	Thus
T16278	10618-10736	sentence	denotes	Thus, the 525A transgene appears to be highly effective at rescuing the neonatal lethal phenotype of the E2 knockout.
T16313	10801-10807	JJ	denotes	lethal
T16312	10792-10800	JJ	denotes	neonatal
T16311	10808-10817	NN	denotes	phenotype
T16310	10788-10791	DT	denotes	the
T16309	10781-10787	VB	denotes	rescue
T16308	10778-10780	TO	denotes	to
T16307	10760-10770	RB	denotes	completely
T16306	10755-10759	NN	denotes	520B
T16305	10750-10754	NN	denotes	line
T16304	10748-10750	,	denotes	,
T16303	10740-10748	NN	denotes	contrast
T16302	10771-10777	VBD	denotes	failed
T16301	10737-10739	IN	denotes	In
T16300	10736-10818	sentence	denotes	In contrast, line 520B completely failed to rescue the neonatal lethal phenotype.
T16299	10735-10736	.	denotes	.
T16298	10724-10726	NN	denotes	E2
T16297	10727-10735	NN	denotes	knockout
T16296	10720-10723	DT	denotes	the
T16295	10717-10719	IN	denotes	of
T16294	10700-10706	JJ	denotes	lethal
T16293	10691-10699	JJ	denotes	neonatal
T16292	10707-10716	NN	denotes	phenotype
T16291	10687-10690	DT	denotes	the
T16290	10678-10686	VBG	denotes	rescuing
T16289	10675-10677	IN	denotes	at
T16288	10665-10674	JJ	denotes	effective
T16287	10658-10664	RB	denotes	highly
T16286	10655-10657	VB	denotes	be
T16355	12117-12118	NNS	denotes	e
T16354	12116-12117	NNS	denotes	l
T16353	12115-12116	JJR	denotes	e
T16352	12114-12115	RB	denotes	s
T16351	12109-12113	VBD	denotes	were
T16350	12109-12118	sentence	denotes	were sele
T16349	12108-12109	sentence	denotes
T16348	12104-12108	NN	denotes	525A
T16347	12102-12103	NN	denotes	d
T16346	12100-12102	NN	denotes	an
T16345	12098-12099	NN	denotes	A
T16344	12098-12108	sentence	denotes	A and 525A
T16343	12096-12097	VBZ	denotes	s
T16342	12095-12096	NN	denotes	e
T16341	12094-12095	NN	denotes	n
T16340	12093-12094	VBZ	denotes	i
T16339	12093-12098	sentence	denotes	ines
T16338	12092-12093	NN	denotes	L
T16379	12153-12255	sentence	denotes	Fig. 4B shows the results of blood amino acid analysis presented as a ratio of total BCAA to alanine.
T16378	12152-12153	.	denotes	.
T16377	12141-12152	NN	denotes	cterization
T16376	12140-12141	VBN	denotes	a
T16375	12139-12140	VBD	denotes	r
T16374	12139-12153	sentence	denotes	racterization.
T16373	12138-12139	NNS	denotes	a
T16372	12137-12138	PRP$	denotes	h
T16371	12136-12137	VBN	denotes	c
T16370	12134-12135	JJ	denotes	d
T16369	12133-12134	PRP	denotes	e
T16368	12132-12133	IN	denotes	l
T16367	12130-12132	NN	denotes	ai
T16366	12129-12130	IN	denotes	t
T16365	12129-12139	sentence	denotes	tailed cha
T16364	12128-12129	NNS	denotes	e
T16363	12127-12128	CC	denotes	d
T16362	12124-12126	NN	denotes	or
T16361	12123-12124	IN	denotes	f
T16360	12123-12129	sentence	denotes	for de
T16359	12122-12123	sentence	denotes
T16358	12121-12122	VBN	denotes	d
T16357	12118-12121	VBZ	denotes	cte
T16356	12118-12122	sentence	denotes	cted
T16382	12162-12167	VBZ	denotes	shows
T16381	12159-12161	NN	denotes	4B
T16380	12154-12158	NN	denotes	Fig.
T16501	13390-13394	VBP	denotes	have
T16500	13381-13386	JJ	denotes	human
T16499	13387-13389	NN	denotes	E2
T16498	13370-13380	JJ	denotes	transgenic
T16497	13367-13369	IN	denotes	of
T16496	13356-13366	NN	denotes	expression
T16495	13350-13355	VB	denotes	drive
T16494	13347-13349	TO	denotes	to
T16493	13337-13341	VBD	denotes	were
T16492	13342-13346	VBN	denotes	used
T16491	13332-13336	WDT	denotes	that
T16490	13322-13323	HYPH	denotes	-
T16489	13323-13326	NN	denotes	tTA
T16488	13319-13322	NN	denotes	LAP
T16487	13327-13331	NNS	denotes	mice
T16486	13315-13318	DT	denotes	the
T16485	13400-13412	VBN	denotes	demonstrated
T16484	13307-13314	IN	denotes	Because
T16483	13307-13555	sentence	denotes	Because the LAP-tTA mice that were used to drive expression of transgenic human E2 have been demonstrated to produce liver specific expression [34], BCKDH enzyme activity and production of human E2 protein in liver of Lines A and 525A was examined.
R2778	T10368	T10367	prep	of,Production
R2779	T10369	T10370	compound	E2,mice
R2780	T10370	T10368	pobj	mice,of
R2781	T10371	T10372	compound	gene,knockout
R2782	T10372	T10370	compound	knockout,mice
R2783	T10374	T10375	aux	To,create
R2784	T10375	T10376	advcl	create,used
R2785	T10377	T10378	compound	E2,mice
R2786	T10378	T10375	dobj	mice,create
R2787	T10379	T10378	compound	knockout,mice
R2788	T10380	T10376	punct	", ",used
R2789	T10381	T10376	nsubj	we,used
R2790	T10382	T10383	compound	gene,targeting
R2791	T10383	T10376	dobj	targeting,used
R2792	T10384	T10376	prep	in,used
R2793	T10385	T10386	compound	mouse,cells
R2794	T10386	T10384	pobj	cells,in
R2795	T10387	T10386	compound	ES,cells
R2796	T10388	T10389	aux	to,disrupt
R2797	T10389	T10376	advcl	disrupt,used
R2798	T10390	T10391	det	the,gene
R2799	T10391	T10389	dobj	gene,disrupt
R2800	T10392	T10391	compound	E2,gene
R2801	T10393	T10376	punct	.,used
R2802	T10395	T10396	det	The,strategy
R2803	T10396	T10398	nsubjpass	strategy,illustrated
R2804	T10397	T10396	amod	overall,strategy
R2805	T10399	T10396	prep	for,strategy
R2806	T10400	T10399	pcomp	disrupting,for
R2807	T10401	T10402	det	the,gene
R2808	T10402	T10400	dobj	gene,disrupting
R2809	T10403	T10402	compound	E2,gene
R2810	T10404	T10398	auxpass	is,illustrated
R2811	T10405	T10398	prep	in,illustrated
R2812	T10406	T10407	compound	Fig.,1A
R2813	T10407	T10405	pobj	1A,in
R2814	T10408	T10398	punct	.,illustrated
R2815	T10410	T10411	det	The,construct
R2816	T10411	T10414	nsubjpass	construct,designed
R2817	T10412	T10413	compound	gene,targeting
R2818	T10413	T10411	compound	targeting,construct
R2819	T10415	T10414	auxpass	was,designed
R2820	T10416	T10417	aux	to,replace
R2821	T10417	T10414	advcl	replace,designed
R2822	T10418	T10419	det	a,fragment
R2823	T10419	T10417	dobj	fragment,replace
R2824	T10420	T10421	nummod	1.67,kb
R2825	T10421	T10419	nmod	kb,fragment
R2826	T10422	T10423	nmod	EcoRV,Smal
R2827	T10423	T10419	nmod	Smal,fragment
R2828	T10424	T10423	punct	/,Smal
R2829	T10425	T10419	amod	genomic,fragment
R2830	T10426	T10419	compound	DNA,fragment
R2831	T10427	T10419	acl	encompassing,fragment
R2832	T10428	T10427	dobj	part,encompassing
R2833	T10429	T10428	prep	of,part
R2834	T10430	T10429	pobj	Exon,of
R2835	T10431	T10430	nummod	4,Exon
R2836	T10432	T10428	cc	and,part
R2837	T10433	T10428	conj	all,part
R2838	T10434	T10433	prep	of,all
R2839	T10435	T10434	pobj	Exon,of
R2840	T10436	T10435	nummod	5,Exon
R2841	T10437	T10417	prep	with,replace
R2842	T10438	T10439	det	the,cassette
R2843	T10439	T10437	pobj	cassette,with
R2844	T10440	T10439	nmod	PGKneo,cassette
R2845	T10441	T10439	amod	selectable,cassette
R2846	T10442	T10439	compound	marker,cassette
R2847	T10443	T10414	punct	.,designed
R2848	T10445	T10446	prep	Of,displayed
R2849	T10447	T10448	nummod	522,clones
R2850	T10448	T10445	pobj	clones,Of
R2851	T10449	T10450	compound	ES,cell
R2852	T10450	T10448	compound	cell,clones
R2853	T10451	T10448	acl	screened,clones
R2854	T10452	T10451	prep	for,screened
R2855	T10453	T10452	pobj	targeting,for
R2856	T10454	T10451	prep	by,screened
R2857	T10455	T10456	compound	Southern,blot
R2858	T10456	T10457	compound	blot,analysis
R2859	T10457	T10454	pobj	analysis,by
R2860	T10458	T10446	punct	", ",displayed
R2861	T10459	T10446	nsubj	29,displayed
R2862	T10460	T10461	punct	(,%
R2863	T10461	T10459	parataxis	%,29
R2864	T10462	T10461	nummod	5,%
R2865	T10463	T10461	punct	),%
R2866	T10464	T10465	det	the,polymorphisms
R2867	T10465	T10446	dobj	polymorphisms,displayed
R2868	T10466	T10465	amod	predicted,polymorphisms
R2869	T10467	T10468	compound	restriction,length
R2870	T10468	T10465	compound	length,polymorphisms
R2871	T10469	T10468	compound	fragment,length
R2872	T10470	T10465	amod	indicative,polymorphisms
R2873	T10471	T10470	prep	of,indicative
R2874	T10472	T10471	pobj	gene,of
R2875	T10473	T10472	amod	targeting,gene
R2876	T10474	T10446	prep	at,displayed
R2877	T10475	T10476	det	the,locus
R2878	T10476	T10474	pobj	locus,at
R2879	T10477	T10476	compound	E2,locus
R2880	T10478	T10446	punct	.,displayed
R2881	T10480	T10481	mark	As,illustrated
R2882	T10481	T10482	advcl	illustrated,hybridizes
R2883	T10483	T10481	prep	in,illustrated
R2884	T10484	T10485	compound	Fig.,1A
R2885	T10485	T10483	pobj	1A,in
R2886	T10486	T10485	cc	and,1A
R2887	T10487	T10485	conj	1B,1A
R2888	T10488	T10482	punct	", ",hybridizes
R2889	T10489	T10490	det	an,probe
R2890	T10490	T10482	nsubj	probe,hybridizes
R2891	T10491	T10490	nmod	E2,probe
R2892	T10492	T10490	nmod	Exon,probe
R2893	T10493	T10492	nummod	6,Exon
R2894	T10494	T10490	amod	specific,probe
R2895	T10495	T10490	punct	", ",probe
R2896	T10496	T10497	dep	which,is
R2897	T10497	T10490	relcl	is,probe
R2898	T10498	T10497	acomp	external,is
R2899	T10499	T10498	prep	to,external
R2900	T10500	T10501	det	the,construct
R2901	T10501	T10499	pobj	construct,to
R2902	T10502	T10501	compound	targeting,construct
R2903	T10503	T10482	punct	", ",hybridizes
R2904	T10504	T10482	prep	to,hybridizes
R2905	T10505	T10506	advmod	only,fragment
R2906	T10506	T10504	pobj	fragment,to
R2907	T10507	T10506	det	a,fragment
R2908	T10508	T10509	punct	~,16
R2909	T10509	T10510	nummod	16,kb
R2910	T10510	T10506	compound	kb,fragment
R2911	T10511	T10506	compound	BglI,fragment
R2912	T10512	T10506	compound	restriction,fragment
R2913	T10513	T10506	prep	from,fragment
R2914	T10514	T10515	det	the,allele
R2915	T10515	T10513	pobj	allele,from
R2916	T10516	T10517	amod	wild,type
R2917	T10517	T10515	compound	type,allele
R2918	T10518	T10515	prep	in,allele
R2919	T10519	T10520	det	the,line
R2920	T10520	T10518	pobj	line,in
R2921	T10521	T10520	amod	parental,line
R2922	T10522	T10520	compound	R1,line
R2923	T10523	T10524	compound	ES,cell
R2924	T10524	T10520	compound	cell,line
R2925	T10525	T10482	punct	.,hybridizes
R2926	T10527	T10528	prep	In,hybridizes
R2927	T10529	T10530	advmod	correctly,targeted
R2928	T10530	T10531	amod	targeted,cells
R2929	T10531	T10527	pobj	cells,In
R2930	T10532	T10531	compound	ES,cells
R2931	T10533	T10528	punct	", ",hybridizes
R2932	T10534	T10535	det	this,probe
R2933	T10535	T10528	nsubj	probe,hybridizes
R2934	T10536	T10528	advmod	also,hybridizes
R2935	T10537	T10528	prep	to,hybridizes
R2936	T10538	T10539	det	a,fragment
R2937	T10539	T10537	pobj	fragment,to
R2938	T10540	T10541	punct	~,11
R2939	T10541	T10542	nummod	11,kb
R2940	T10542	T10539	compound	kb,fragment
R2941	T10543	T10539	compound	BglI,fragment
R2942	T10544	T10539	compound	restriction,fragment
R2943	T10545	T10528	punct	.,hybridizes
R2944	T10547	T10548	nsubjpass	Targeting,confirmed
R2945	T10549	T10548	auxpass	was,confirmed
R2946	T10550	T10548	prep	with,confirmed
R2947	T10551	T10552	amod	several,enzymes
R2948	T10552	T10550	pobj	enzymes,with
R2949	T10553	T10552	amod	additional,enzymes
R2950	T10554	T10552	compound	restriction,enzymes
R2951	T10555	T10552	cc	and,enzymes
R2952	T10556	T10552	conj	probes,enzymes
R2953	T10557	T10558	punct	(,shown
R2954	T10558	T10548	parataxis	shown,confirmed
R2955	T10559	T10558	nsubj	data,shown
R2956	T10560	T10558	neg	not,shown
R2957	T10561	T10558	punct	),shown
R2958	T10562	T10548	punct	.,confirmed
R2959	T10564	T10565	advmod	Correctly,targeted
R2960	T10565	T10566	amod	targeted,cells
R2961	T10566	T10568	nsubjpass	cells,microinjected
R2962	T10567	T10566	compound	ES,cells
R2963	T10569	T10568	auxpass	were,microinjected
R2964	T10570	T10568	prep	into,microinjected
R2965	T10571	T10570	pobj	blastocysts,into
R2966	T10572	T10573	aux	to,produce
R2967	T10573	T10568	advcl	produce,microinjected
R2968	T10574	T10575	amod	chimeric,mice
R2969	T10575	T10573	dobj	mice,produce
R2970	T10576	T10568	punct	.,microinjected
R2971	T10578	T10579	nsubjpass	Chimerics,bred
R2972	T10580	T10579	auxpass	were,bred
R2973	T10581	T10579	prep	to,bred
R2974	T10582	T10583	compound	C57BL,6J
R2975	T10583	T10585	compound	6J,mice
R2976	T10584	T10583	punct	/,6J
R2977	T10585	T10581	pobj	mice,to
R2978	T10586	T10579	punct	.,bred
R2979	T10588	T10589	prep	Following,interbred
R2980	T10590	T10591	compound	germline,transmission
R2981	T10591	T10588	pobj	transmission,Following
R2982	T10592	T10591	prep	of,transmission
R2983	T10593	T10594	det	the,allele
R2984	T10594	T10592	pobj	allele,of
R2985	T10595	T10594	amod	targeted,allele
R2986	T10596	T10589	punct	", ",interbred
R2987	T10597	T10598	amod	heterozygous,mice
R2988	T10598	T10589	nsubjpass	mice,interbred
R2989	T10599	T10589	auxpass	were,interbred
R2990	T10600	T10601	aux	to,produce
R2991	T10601	T10589	advcl	produce,interbred
R2992	T10602	T10603	amod	wild,type
R2993	T10603	T10604	nmod	type,animals
R2994	T10604	T10601	dobj	animals,produce
R2995	T10605	T10606	punct	(,+
R2996	T10606	T10603	punct	+,type
R2997	T10607	T10606	punct	+,+
R2998	T10608	T10606	punct	/,+
R2999	T10609	T10606	punct	),+
R3000	T10610	T10603	punct	", ",type
R3001	T10611	T10603	conj	heterozygous,type
R3002	T10612	T10613	punct	(,-
R3003	T10613	T10611	punct	-,heterozygous
R3004	T10614	T10613	punct	+,-
R3005	T10615	T10613	punct	/,-
R3006	T10616	T10613	punct	),-
R3007	T10617	T10611	cc	and,heterozygous
R3008	T10618	T10611	conj	homozygous,heterozygous
R3009	T10619	T10620	punct	(,-
R3010	T10620	T10618	punct	-,homozygous
R3011	T10621	T10620	punct	-,-
R3012	T10622	T10620	punct	/,-
R3013	T10623	T10620	punct	),-
R3014	T10624	T10589	punct	.,interbred
R3015	T10626	T10627	nsubjpass	Mice,genotyped
R3016	T10628	T10627	auxpass	were,genotyped
R3017	T10629	T10627	prep	by,genotyped
R3018	T10630	T10631	compound	Southern,blot
R3019	T10631	T10632	compound	blot,analysis
R3020	T10632	T10629	pobj	analysis,by
R3021	T10633	T10627	punct	.,genotyped
R3022	T10635	T10636	det	The,probe
R3023	T10636	T10640	nsubj	probe,hybridized
R3024	T10637	T10638	npadvmod	Exon,specific
R3025	T10638	T10636	amod	specific,probe
R3026	T10639	T10637	nummod	6,Exon
R3027	T10641	T10640	prep	to,hybridized
R3028	T10642	T10643	advmod	only,fragment
R3029	T10643	T10641	pobj	fragment,to
R3030	T10644	T10643	det	a,fragment
R3031	T10645	T10646	punct	~,16
R3032	T10646	T10647	nummod	16,kb
R3033	T10647	T10643	compound	kb,fragment
R3034	T10648	T10643	compound	BglI,fragment
R3035	T10649	T10643	compound	restriction,fragment
R3036	T10650	T10643	prep	in,fragment
R3037	T10651	T10652	punct	+,+
R3038	T10652	T10654	punct	+,mice
R3039	T10653	T10652	punct	/,+
R3040	T10654	T10650	pobj	mice,in
R3041	T10655	T10643	punct	", ",fragment
R3042	T10656	T10657	det	a,fragment
R3043	T10657	T10643	appos	fragment,fragment
R3044	T10658	T10659	punct	~,11
R3045	T10659	T10660	nummod	11,kb
R3046	T10660	T10657	compound	kb,fragment
R3047	T10661	T10657	compound	BglI,fragment
R3048	T10662	T10657	prep	in,fragment
R3049	T10663	T10664	punct	-,-
R3050	T10664	T10666	punct	-,mice
R3051	T10665	T10664	punct	/,-
R3052	T10666	T10662	pobj	mice,in
R3053	T10667	T10641	punct	", ",to
R3054	T10668	T10641	cc	and,to
R3055	T10669	T10641	conj	to,to
R3056	T10670	T10669	pobj	both,to
R3057	T10671	T10670	prep	of,both
R3058	T10672	T10673	det	these,fragments
R3059	T10673	T10671	pobj	fragments,of
R3060	T10674	T10670	prep	in,both
R3061	T10675	T10676	punct	+,-
R3062	T10676	T10678	punct	-,mice
R3063	T10677	T10676	punct	/,-
R3064	T10678	T10674	pobj	mice,in
R3065	T10679	T10680	punct	(,1B
R3066	T10680	T10640	parataxis	1B,hybridized
R3067	T10681	T10680	compound	Fig.,1B
R3068	T10682	T10680	punct	),1B
R3069	T10683	T10640	punct	.,hybridized
R3070	T10685	T10686	nsubjpass	Mice,born
R3071	T10687	T10685	amod	homozygous,Mice
R3072	T10688	T10687	prep	for,homozygous
R3073	T10689	T10690	det	the,mutation
R3074	T10690	T10688	pobj	mutation,for
R3075	T10691	T10690	compound	E2,mutation
R3076	T10692	T10686	auxpass	were,born
R3077	T10693	T10686	prep	at,born
R3078	T10694	T10695	det	the,frequency
R3079	T10695	T10693	pobj	frequency,at
R3080	T10696	T10695	amod	expected,frequency
R3081	T10697	T10686	punct	.,born
R3082	T10699	T10700	compound	Genotype,analysis
R3083	T10700	T10701	nsubj	analysis,revealed
R3084	T10702	T10700	prep	of,analysis
R3085	T10703	T10702	pobj	pups,of
R3086	T10704	T10703	acl	derived,pups
R3087	T10705	T10704	prep	from,derived
R3088	T10706	T10707	punct	+,-
R3089	T10707	T10709	punct	-,matings
R3090	T10708	T10707	punct	/,-
R3091	T10709	T10705	pobj	matings,from
R3092	T10710	T10707	prep	by,-
R3093	T10711	T10712	punct	+,-
R3094	T10712	T10710	punct	-,by
R3095	T10713	T10712	punct	/,-
R3096	T10714	T10715	mark	that,were
R3097	T10715	T10701	ccomp	were,revealed
R3098	T10716	T10717	punct	+,+
R3099	T10717	T10719	punct	+,mice
R3100	T10718	T10717	punct	/,+
R3101	T10719	T10715	nsubj	mice,were
R3102	T10720	T10717	punct	", ",+
R3103	T10721	T10722	punct	+,-
R3104	T10722	T10717	conj	-,+
R3105	T10723	T10722	punct	/,-
R3106	T10724	T10722	punct	", ",-
R3107	T10725	T10722	cc	and,-
R3108	T10726	T10727	punct	-,-
R3109	T10727	T10722	conj	-,-
R3110	T10728	T10727	punct	/,-
R3111	T10729	T10715	acomp	present,were
R3112	T10730	T10715	prep	at,were
R3113	T10731	T10732	advmod	nearly,frequency
R3114	T10732	T10730	pobj	frequency,at
R3115	T10733	T10732	det	the,frequency
R3116	T10734	T10732	amod	expected,frequency
R3117	T10735	T10736	nummod	1,1
R3118	T10736	T10732	nummod	1,frequency
R3119	T10737	T10736	punct	:,1
R3120	T10738	T10736	nummod	2,1
R3121	T10739	T10736	punct	:,1
R3122	T10740	T10701	punct	.,revealed
R3123	T10742	T10743	prep	Of,were
R3124	T10744	T10745	det	the,animals
R3125	T10745	T10742	pobj	animals,Of
R3126	T10746	T10745	amod	initial,animals
R3127	T10747	T10745	nummod	60,animals
R3128	T10748	T10745	acl	genotyped,animals
R3129	T10749	T10743	punct	", ",were
R3130	T10750	T10743	nsubj	19,were
R3131	T10751	T10752	punct	(,%
R3132	T10752	T10750	parataxis	%,19
R3133	T10753	T10752	nummod	32,%
R3134	T10754	T10752	punct	),%
R3135	T10755	T10756	punct	+,+
R3136	T10756	T10743	attr	+,were
R3137	T10757	T10756	punct	/,+
R3138	T10758	T10743	punct	", ",were
R3139	T10759	T10760	nsubj	27,were
R3140	T10760	T10743	conj	were,were
R3141	T10761	T10762	punct	(,%
R3142	T10762	T10759	parataxis	%,27
R3143	T10763	T10762	nummod	45,%
R3144	T10764	T10762	punct	),%
R3145	T10765	T10766	punct	+,-
R3146	T10766	T10760	attr	-,were
R3147	T10767	T10766	punct	/,-
R3148	T10768	T10760	punct	", ",were
R3149	T10769	T10760	cc	and,were
R3150	T10770	T10771	nsubj	14,were
R3151	T10771	T10760	conj	were,were
R3152	T10772	T10773	punct	(,%
R3153	T10773	T10770	parataxis	%,14
R3154	T10774	T10773	nummod	23,%
R3155	T10775	T10773	punct	),%
R3156	T10776	T10777	punct	-,-
R3157	T10777	T10771	attr	-,were
R3158	T10778	T10777	punct	/,-
R3159	T10779	T10743	punct	.,were
R3160	T10781	T10782	advmod	Thus,was
R3161	T10783	T10782	punct	", ",was
R3162	T10784	T10785	det	the,gene
R3163	T10785	T10782	nsubj	gene,was
R3164	T10786	T10785	compound	E2,gene
R3165	T10787	T10782	acomp	dispensable,was
R3166	T10788	T10787	prep	for,dispensable
R3167	T10789	T10790	amod	normal,development
R3168	T10790	T10788	pobj	development,for
R3169	T10791	T10790	amod	embryonic,development
R3170	T10792	T10782	punct	.,was
R3171	T10794	T10795	advmod	However,died
R3172	T10796	T10795	punct	", ",died
R3173	T10797	T10798	mark	as,expected
R3174	T10798	T10795	advcl	expected,died
R3175	T10799	T10795	punct	", ",died
R3176	T10800	T10801	advmod	nearly,mice
R3177	T10801	T10795	nsubj	mice,died
R3178	T10802	T10801	det	all,mice
R3179	T10803	T10801	amod	homozygous,mice
R3180	T10804	T10795	prep	in,died
R3181	T10805	T10806	det	the,period
R3182	T10806	T10804	pobj	period,in
R3183	T10807	T10806	amod	perinatal,period
R3184	T10808	T10795	punct	.,died
R3185	T10810	T10811	advmod	Immediately,following
R3186	T10811	T10812	prep	following,were
R3187	T10812	T10817	ccomp	were,were
R3188	T10813	T10811	pobj	birth,following
R3189	T10814	T10812	punct	", ",were
R3190	T10815	T10816	amod	homozygous,pups
R3191	T10816	T10812	nsubj	pups,were
R3192	T10818	T10812	acomp	indistinguishable,were
R3193	T10819	T10818	prep	from,indistinguishable
R3194	T10820	T10821	poss	their,littermates
R3195	T10821	T10819	pobj	littermates,from
R3196	T10822	T10823	punct	+,+
R3197	T10823	T10821	punct	+,littermates
R3198	T10824	T10823	punct	/,+
R3199	T10825	T10823	cc	and,+
R3200	T10826	T10827	punct	+,-
R3201	T10827	T10823	conj	-,+
R3202	T10828	T10827	punct	/,-
R3203	T10829	T10817	punct	;,were
R3204	T10830	T10817	nsubj	they,were
R3205	T10831	T10817	acomp	vigorous,were
R3206	T10832	T10831	punct	", ",vigorous
R3207	T10833	T10831	conj	active,vigorous
R3208	T10834	T10833	cc	and,active
R3209	T10835	T10833	conj	able,active
R3210	T10836	T10837	aux	to,suckle
R3211	T10837	T10835	xcomp	suckle,able
R3212	T10838	T10817	punct	.,were
R3213	T10840	T10841	prep	By,became
R3214	T10842	T10843	amod	mid,day
R3215	T10843	T10840	pobj	day,By
R3216	T10844	T10842	prep	to,mid
R3217	T10845	T10844	amod	late,to
R3218	T10846	T10843	prep	on,day
R3219	T10847	T10848	amod	postnatal,day
R3220	T10848	T10846	pobj	day,on
R3221	T10849	T10848	nummod	one,day
R3222	T10850	T10841	punct	", ",became
R3223	T10851	T10852	advmod	most,pups
R3224	T10852	T10841	nsubj	pups,became
R3225	T10853	T10854	punct	-,-
R3226	T10854	T10852	punct	-,pups
R3227	T10855	T10854	punct	/,-
R3228	T10856	T10841	acomp	moribund,became
R3229	T10857	T10841	cc	and,became
R3230	T10858	T10841	conj	were,became
R3231	T10859	T10860	advmod	readily,identifiable
R3232	T10860	T10858	acomp	identifiable,were
R3233	T10861	T10862	mark	as,were
R3234	T10862	T10860	advcl	were,identifiable
R3235	T10863	T10862	nsubj	they,were
R3236	T10864	T10865	amod	lethargic,pale
R3237	T10865	T10862	acomp	pale,were
R3238	T10866	T10865	punct	", ",pale
R3239	T10867	T10862	punct	", ",were
R3240	T10868	T10862	cc	and,were
R3241	T10869	T10862	conj	exhibited,were
R3242	T10870	T10871	amod	gasping,movements
R3243	T10871	T10869	dobj	movements,exhibited
R3244	T10872	T10871	amod	respiratory,movements
R3245	T10873	T10841	punct	.,became
R3246	T10875	T10876	prep	With,died
R3247	T10877	T10878	amod	few,exceptions
R3248	T10878	T10875	pobj	exceptions,With
R3249	T10879	T10876	punct	", ",died
R3250	T10880	T10881	punct	-,-
R3251	T10881	T10883	punct	-,pups
R3252	T10882	T10881	punct	/,-
R3253	T10883	T10876	nsubj	pups,died
R3254	T10884	T10876	prep	within,died
R3255	T10885	T10886	nummod	72,hours
R3256	T10886	T10884	pobj	hours,within
R3257	T10887	T10886	prep	of,hours
R3258	T10888	T10887	pobj	birth,of
R3259	T10889	T10876	punct	.,died
R3260	T10891	T10892	nsubj	We,observed
R3261	T10893	T10892	aux	have,observed
R3262	T10894	T10895	nummod	one,pup
R3263	T10895	T10892	dobj	pup,observed
R3264	T10896	T10895	amod	rare,pup
R3265	T10897	T10898	punct	-,-
R3266	T10898	T10895	punct	-,pup
R3267	T10899	T10898	punct	/,-
R3268	T10900	T10901	dep	that,survived
R3269	T10901	T10895	relcl	survived,pup
R3270	T10902	T10901	prep	to,survived
R3271	T10903	T10904	amod	postnatal,day
R3272	T10904	T10902	pobj	day,to
R3273	T10905	T10904	nummod	13,day
R3274	T10906	T10892	punct	.,observed
R3275	T10908	T10909	det	The,reason
R3276	T10909	T10910	nsubj	reason,is
R3277	T10911	T10909	prep	for,reason
R3278	T10912	T10913	det	the,survival
R3279	T10913	T10911	pobj	survival,for
R3280	T10914	T10913	amod	prolonged,survival
R3281	T10915	T10913	prep	of,survival
R3282	T10916	T10917	det	this,pup
R3283	T10917	T10915	pobj	pup,of
R3284	T10918	T10910	acomp	unknown,is
R3285	T10919	T10910	punct	.,is
R3290	T12206	T12207	npadvmod	E2,deficient
R3291	T12207	T12208	amod	deficient,mice
R3292	T12208	T12209	nsubj	mice,model
R3293	T12210	T12209	advmod	accurately,model
R3294	T12211	T12212	amod	classic,MSUD
R3295	T12212	T12209	dobj	MSUD,model
R3296	T12214	T12215	aux	To,demonstrate
R3297	T12215	T12216	advcl	demonstrate,determined
R3298	T12217	T12218	mark	that,created
R3299	T12218	T12215	ccomp	created,demonstrate
R3300	T12219	T12220	det	the,event
R3301	T12220	T12218	nsubj	event,created
R3302	T12221	T12222	compound	gene,targeting
R3303	T12222	T12220	compound	targeting,event
R3304	T12223	T12224	det	a,allele
R3305	T12224	T12218	dobj	allele,created
R3306	T12225	T12224	amod	true,allele
R3307	T12226	T12227	npadvmod	E2,null
R3308	T12227	T12224	amod	null,allele
R3309	T12228	T12216	punct	", ",determined
R3310	T12229	T12216	nsubj	we,determined
R3311	T12230	T12231	compound	BCKDH,activity
R3312	T12231	T12216	dobj	activity,determined
R3313	T12232	T12216	prep	in,determined
R3314	T12233	T12234	compound	liver,homogenates
R3315	T12234	T12232	pobj	homogenates,in
R3316	T12235	T12234	prep	of,homogenates
R3317	T12236	T12237	amod	postnatal,day
R3318	T12237	T12238	nmod	day,pups
R3319	T12238	T12235	pobj	pups,of
R3320	T12239	T12237	nummod	1,day
R3321	T12240	T12238	compound	mouse,pups
R3322	T12241	T12238	acl	derived,pups
R3323	T12242	T12241	prep	from,derived
R3324	T12243	T12244	punct	+,-
R3325	T12244	T12246	punct	-,pairs
R3326	T12245	T12244	punct	/,-
R3327	T12246	T12242	pobj	pairs,from
R3328	T12247	T12244	prep	by,-
R3329	T12248	T12249	punct	+,-
R3330	T12249	T12247	punct	-,by
R3331	T12250	T12249	punct	/,-
R3332	T12251	T12246	compound	mating,pairs
R3333	T12252	T12216	punct	.,determined
R3334	T12254	T12255	mark	As,shown
R3335	T12255	T12256	advcl	shown,was
R3336	T12257	T12255	prep	in,shown
R3337	T12258	T12259	compound	Figure,2A
R3338	T12259	T12257	pobj	2A,in
R3339	T12260	T12256	punct	", ",was
R3340	T12261	T12262	det	the,activity
R3341	T12262	T12256	nsubj	activity,was
R3342	T12263	T12262	compound	BCKDH,activity
R3343	T12264	T12262	prep	in,activity
R3344	T12265	T12266	punct	+,+
R3346	T12267	T12266	punct	/,+
R3363	T12286	T12282	pobj	livers,in
R3364	T12287	T12286	compound	mouse,livers
R3365	T12288	T12274	punct	.,was
R3366	T12290	T12291	mark	As,expected
R3367	T12291	T12292	advcl	expected,had
R3368	T12293	T12292	punct	", ",had
R3369	T12294	T12292	nsubj	homogenates,had
R3370	T12295	T12294	prep	from,homogenates
R3371	T12296	T12297	punct	+,-
R3372	T12297	T12299	punct	-,mice
R3373	T12298	T12297	punct	/,-
R3374	T12299	T12295	pobj	mice,from
R3375	T12300	T12301	advmod	approximately,half
R3376	T12301	T12302	nummod	half,activity
R3377	T12302	T12292	dobj	activity,had
R3378	T12303	T12302	det	the,activity
R3379	T12304	T12302	prep	of,activity
R3380	T12305	T12306	poss	their,littermates
R3381	T12306	T12304	pobj	littermates,of
R3382	T12307	T12308	punct	+,+
R3383	T12308	T12306	punct	+,littermates
R3384	T12309	T12308	punct	/,+
R3385	T12310	T12311	punct	(,2A
R3386	T12311	T12292	parataxis	2A,had
R3387	T12312	T12311	compound	Figure,2A
R3388	T12313	T12311	punct	),2A
R3389	T12314	T12292	punct	.,had
R3390	T12316	T12317	det	The,results
R3391	T12317	T12318	nsubj	results,are
R3392	T12319	T12317	prep	from,results
R3393	T12320	T12321	punct	-,-
R3394	T12321	T12323	punct	-,mice
R3395	T12322	T12321	punct	/,-
R3396	T12323	T12319	pobj	mice,from
R3397	T12324	T12318	acomp	similar,are
R3398	T12325	T12324	prep	to,similar
R3399	T12326	T12325	pobj	that,to
R3400	T12327	T12326	acl	observed,that
R3401	T12328	T12327	prep	in,observed
R3402	T12329	T12328	pobj	humans,in
R3403	T12330	T12329	prep	with,humans
R3404	T12331	T12332	amod	classic,MSUD
R3405	T12332	T12330	pobj	MSUD,with
R3406	T12333	T12334	punct	[,1
R3407	T12334	T12318	parataxis	1,are
R3408	T12335	T12334	punct	],1
R3409	T12336	T12318	punct	.,are
R3420	T12350	T12348	dobj	protein,examine
R3421	T12351	T12348	prep	in,examine
R3422	T12352	T12353	det	the,mice
R3423	T12353	T12351	pobj	mice,in
R3424	T12354	T12340	punct	.,used
R3425	T12356	T12357	mark	As,shown
R3426	T12357	T12358	advcl	shown,was
R3427	T12359	T12357	prep	in,shown
R3428	T12360	T12361	compound	Figure,1C
R3429	T12361	T12359	pobj	1C,in
R3430	T12362	T12361	cc	and,1C
R3431	T12363	T12361	conj	1D,1C
R3432	T12364	T12358	punct	", ",was
R3433	T12365	T12366	amod	immunoreactive,protein
R3434	T12366	T12358	nsubj	protein,was
R3435	T12367	T12366	compound	E2,protein
R3436	T12368	T12358	acomp	abundant,was
R3437	T12369	T12358	prep	in,was
R3438	T12370	T12369	pobj	liver,in
R3439	T12371	T12370	cc	and,liver
R3440	T12372	T12373	amod	embryonic,fibroblasts
R3441	T12373	T12370	conj	fibroblasts,liver
R3442	T12374	T12370	prep	of,liver
R3443	T12375	T12376	punct	+,+
R3444	T12376	T12378	punct	+,mice
R3445	T12377	T12376	punct	/,+
R3446	T12378	T12374	pobj	mice,of
R3447	T12379	T12358	punct	.,was
R3448	T12381	T12382	prep	In,was
R3449	T12383	T12384	amod	marked,contrast
R3450	T12384	T12381	pobj	contrast,In
R3451	T12385	T12382	punct	", ",was
R3452	T12386	T12387	amod	immunoreactive,protein
R3453	T12387	T12382	nsubj	protein,was
R3454	T12388	T12387	compound	E2,protein
R3455	T12389	T12382	acomp	absent,was
R3456	T12390	T12382	prep	in,was
R3457	T12391	T12392	det	these,tissues
R3458	T12392	T12390	pobj	tissues,in
R3459	T12393	T12392	amod	same,tissues
R3460	T12394	T12392	prep	of,tissues
R3461	T12395	T12396	punct	-,-
R3462	T12396	T12398	punct	-,mice
R3463	T12397	T12396	punct	/,-
R3464	T12398	T12394	pobj	mice,of
R3465	T12399	T12382	punct	.,was
R3466	T12401	T12402	amod	Homozygous,knockout
R3467	T12402	T12404	compound	knockout,mice
R3468	T12403	T12402	compound	E2,knockout
R3469	T12404	T12405	nsubj	mice,had
R3470	T12406	T12407	det	a,increase
R3471	T12407	T12405	dobj	increase,had
R3472	T12408	T12409	advmod	nearly,3
R3473	T12409	T12410	quantmod	3,fold
R3474	T12410	T12407	nummod	fold,increase
R3475	T12411	T12410	punct	-,fold
R3476	T12412	T12407	prep	in,increase
R3477	T12413	T12414	nmod	blood,levels
R3478	T12414	T12412	pobj	levels,in
R3479	T12415	T12416	punct	(,2B
R3480	T12416	T12413	parataxis	2B,blood
R3481	T12417	T12416	compound	Figure,2B
R3482	T12418	T12416	punct	),2B
R3483	T12419	T12413	cc	and,blood
R3484	T12420	T12413	conj	urine,blood
R3485	T12421	T12422	punct	(,shown
R3486	T12422	T12420	parataxis	shown,urine
R3487	T12423	T12422	nsubj	data,shown
R3488	T12424	T12422	neg	not,shown
R3489	T12425	T12422	punct	),shown
R3490	T12426	T12414	prep	of,levels
R3491	T12427	T12426	pobj	BCAA,of
R3492	T12428	T12429	punct	(,sum
R3493	T12429	T12427	parataxis	sum,BCAA
R3494	T12430	T12429	prep	of,sum
R3495	T12431	T12430	pobj	leucine,of
R3496	T12432	T12431	punct	", ",leucine
R3497	T12433	T12431	conj	isoleucine,leucine
R3498	T12434	T12433	punct	", ",isoleucine
R3499	T12435	T12433	cc	and,isoleucine
R3500	T12436	T12433	conj	valine,isoleucine
R3501	T12437	T12429	punct	),sum
R3502	T12438	T12407	prep	as,increase
R3503	T12439	T12438	prep	compared,as
R3504	T12440	T12439	prep	to,compared
R3505	T12441	T12442	poss	their,littermates
R3506	T12442	T12440	pobj	littermates,to
R3507	T12443	T12444	punct	+,+
R3508	T12444	T12442	punct	+,littermates
R3509	T12445	T12444	punct	/,+
R3510	T12446	T12405	punct	.,had
R3511	T12448	T12449	mark	Because,analyzed
R3512	T12449	T12453	advcl	analyzed,include
R3513	T12450	T12451	compound	amino,acids
R3514	T12451	T12449	nsubjpass	acids,analyzed
R3515	T12452	T12449	auxpass	were,analyzed
R3516	T12454	T12449	prep	by,analyzed
R3517	T12455	T12456	compound	tandem,spectrometry
R3518	T12456	T12454	pobj	spectrometry,by
R3519	T12457	T12456	compound	mass,spectrometry
R3520	T12458	T12453	punct	", ",include
R3521	T12459	T12460	det	the,sum
R3522	T12460	T12453	nsubj	sum,include
R3523	T12461	T12460	prep	of,sum
R3524	T12462	T12461	pobj	BCAA,of
R3525	T12463	T12460	acl	shown,sum
R3526	T12464	T12463	prep	in,shown
R3527	T12465	T12466	compound	Figure,2B
R3528	T12466	T12464	pobj	2B,in
R3529	T12467	T12453	advmod	also,include
R3530	T12468	T12453	aux	may,include
R3531	T12469	T12453	dobj	alloisoleucine,include
R3532	T12470	T12471	dep	that,produced
R3533	T12471	T12469	relcl	produced,alloisoleucine
R3534	T12472	T12471	aux	may,produced
R3535	T12473	T12471	aux	have,produced
R3536	T12474	T12471	auxpass	been,produced
R3537	T12475	T12471	prep	in,produced
R3538	T12476	T12477	punct	-,-
R3539	T12477	T12479	punct	-,mice
R3540	T12478	T12477	punct	/,-
R3541	T12479	T12475	pobj	mice,in
R3542	T12480	T12479	compound	MSUD,mice
R3543	T12481	T12453	punct	.,include
R3544	T12483	T12484	det	The,metabolism
R3545	T12484	T12485	nsubjpass	metabolism,linked
R3546	T12486	T12484	prep	of,metabolism
R3547	T12487	T12486	pobj	BCAA,of
R3548	T12488	T12485	auxpass	is,linked
R3549	T12489	T12485	prep	with,linked
R3550	T12490	T12491	det	the,synthesis
R3551	T12491	T12489	pobj	synthesis,with
R3552	T12492	T12491	prep	of,synthesis
R3553	T12493	T12492	pobj	alanine,of
R3554	T12494	T12493	punct	", ",alanine
R3555	T12495	T12493	conj	glutamate,alanine
R3556	T12496	T12495	punct	", ",glutamate
R3557	T12497	T12495	cc	and,glutamate
R3558	T12498	T12495	conj	glutamine,glutamate
R3559	T12499	T12500	punct	[,33
R3560	T12500	T12485	parataxis	33,linked
R3561	T12501	T12500	punct	],33
R3562	T12502	T12485	punct	.,linked
R3563	T12504	T12505	prep	Because,analyzed
R3564	T12506	T12504	pcomp	of,Because
R3565	T12507	T12508	amod	impaired,metabolism
R3566	T12508	T12504	pobj	metabolism,Because
R3567	T12509	T12508	prep	of,metabolism
R3568	T12510	T12509	pobj	BCAA,of
R3569	T12511	T12508	prep	in,metabolism
R3570	T12512	T12513	compound	MSUD,mice
R3571	T12513	T12511	pobj	mice,in
R3572	T12514	T12504	punct	", ",Because
R3573	T12515	T12504	cc	and,Because
R3574	T12516	T12517	aux	to,characterize
R3575	T12517	T12504	conj	characterize,Because
R3576	T12518	T12517	advmod	further,characterize
R3577	T12519	T12520	det	the,biochemistry
R3578	T12520	T12517	dobj	biochemistry,characterize
R3579	T12521	T12520	amod	abnormal,biochemistry
R3580	T12522	T12517	prep	in,characterize
R3581	T12523	T12524	det	this,model
R3582	T12524	T12522	pobj	model,in
R3583	T12525	T12505	punct	", ",analyzed
R3584	T12526	T12505	nsubj	we,analyzed
R3585	T12527	T12528	det	the,levels
R3586	T12528	T12505	dobj	levels,analyzed
R3587	T12529	T12528	compound	blood,levels
R3588	T12530	T12528	prep	of,levels
R3589	T12531	T12532	det	the,alanine
R3590	T12532	T12530	pobj	alanine,of
R3591	T12533	T12532	punct	", ",alanine
R3592	T12534	T12532	conj	glutamate,alanine
R3593	T12535	T12534	punct	", ",glutamate
R3594	T12536	T12534	cc	and,glutamate
R3595	T12537	T12534	conj	glutamine,glutamate
R3596	T12538	T12505	punct	.,analyzed
R3597	T12540	T12541	mark	As,shown
R3598	T12541	T12542	advcl	shown,were
R3599	T12543	T12541	prep	in,shown
R3600	T12544	T12543	pobj	Table,in
R3601	T12545	T12544	nummod	1,Table
R3602	T12546	T12542	punct	", ",were
R3603	T12547	T12548	det	the,levels
R3604	T12548	T12542	nsubj	levels,were
R3605	T12549	T12548	prep	of,levels
R3606	T12550	T12551	det	all,acids
R3607	T12551	T12549	pobj	acids,of
R3608	T12552	T12551	nummod	three,acids
R3609	T12553	T12551	compound	amino,acids
R3610	T12554	T12548	prep	in,levels
R3611	T12555	T12556	amod	homozygous,mice
R3612	T12556	T12554	pobj	mice,in
R3613	T12557	T12558	advmod	markedly,lower
R3614	T12558	T12542	acomp	lower,were
R3615	T12559	T12558	prep	than,lower
R3616	T12560	T12561	det	the,levels
R3617	T12561	T12559	pobj	levels,than
R3618	T12562	T12561	prep	in,levels
R3619	T12563	T12564	punct	+,+
R3620	T12564	T12566	punct	+,mice
R3621	T12565	T12564	punct	/,+
R3622	T12566	T12562	pobj	mice,in
R3623	T12567	T12564	cc	or,+
R3624	T12568	T12569	punct	+,-
R3625	T12569	T12564	conj	-,+
R3626	T12570	T12569	punct	/,-
R3627	T12571	T12542	punct	.,were
R3628	T12573	T12574	det	The,levels
R3629	T12574	T12575	nsubj	levels,were
R3630	T12576	T12574	prep	of,levels
R3631	T12577	T12578	det	these,acids
R3632	T12578	T12576	pobj	acids,of
R3633	T12579	T12578	compound	amino,acids
R3634	T12580	T12574	prep	in,levels
R3635	T12581	T12582	det	the,mice
R3636	T12582	T12580	pobj	mice,in
R3637	T12583	T12584	punct	+,-
R3638	T12584	T12582	punct	-,mice
R3639	T12585	T12584	punct	/,-
R3640	T12586	T12575	acomp	comparable,were
R3641	T12587	T12586	prep	to,comparable
R3642	T12588	T12587	pobj	those,to
R3643	T12589	T12588	prep	in,those
R3644	T12590	T12591	punct	+,+
R3645	T12591	T12593	punct	+,mice
R3646	T12592	T12591	punct	/,+
R3647	T12593	T12589	pobj	mice,in
R3648	T12594	T12595	punct	(,Table
R3649	T12595	T12575	parataxis	Table,were
R3650	T12596	T12595	nummod	1,Table
R3651	T12597	T12595	punct	),Table
R3652	T12598	T12575	punct	.,were
R3653	T12600	T12601	prep	Because,suggested
R3654	T12602	T12600	pcomp	of,Because
R3655	T12603	T12604	det	the,decrease
R3656	T12604	T12600	pobj	decrease,Because
R3657	T12605	T12604	amod	abnormal,decrease
R3658	T12606	T12604	prep	in,decrease
R3659	T12607	T12608	det	the,level
R3660	T12608	T12606	pobj	level,in
R3661	T12609	T12608	compound	blood,level
R3662	T12610	T12608	compound	alanine,level
R3663	T12611	T12604	cc	and,decrease
R3664	T12612	T12613	amod	marked,rise
R3665	T12613	T12604	conj	rise,decrease
R3666	T12614	T12613	prep	in,rise
R3667	T12615	T12616	compound	blood,levels
R3668	T12616	T12614	pobj	levels,in
R3669	T12617	T12616	compound	BCAA,levels
R3670	T12618	T12619	dep	that,are
R3671	T12619	T12604	relcl	are,decrease
R3672	T12620	T12619	acomp	characteristic,are
R3673	T12621	T12620	prep	of,characteristic
R3674	T12622	T12621	pobj	MSUD,of
R3675	T12623	T12601	punct	", ",suggested
R3676	T12624	T12625	det	a,report
R3677	T12625	T12601	nsubj	report,suggested
R3678	T12626	T12625	amod	recent,report
R3679	T12627	T12625	prep	on,report
R3680	T12628	T12629	compound	MSUD,patients
R3681	T12629	T12627	pobj	patients,on
R3682	T12630	T12601	aux	has,suggested
R3683	T12631	T12632	mark	that,provides
R3684	T12632	T12601	ccomp	provides,suggested
R3685	T12633	T12634	det	the,ratio
R3686	T12634	T12632	nsubj	ratio,provides
R3687	T12635	T12634	prep	of,ratio
R3688	T12636	T12637	compound	BCAA,alanine
R3689	T12637	T12635	pobj	alanine,of
R3690	T12638	T12637	punct	/,alanine
R3691	T12639	T12640	det	a,measure
R3692	T12640	T12632	dobj	measure,provides
R3693	T12641	T12642	advmod	more,sensitive
R3694	T12642	T12640	amod	sensitive,measure
R3695	T12643	T12640	prep	of,measure
R3696	T12644	T12645	det	the,biochemistry
R3697	T12645	T12643	pobj	biochemistry,of
R3698	T12646	T12645	amod	abnormal,biochemistry
R3699	T12647	T12645	prep	of,biochemistry
R3700	T12648	T12647	pobj	MSUD,of
R3701	T12649	T12640	prep	than,measure
R3702	T12650	T12651	compound	BCAA,level
R3703	T12651	T12649	pobj	level,than
R3704	T12652	T12651	advmod	alone,level
R3705	T12653	T12654	punct	[,8
R3706	T12654	T12601	parataxis	8,suggested
R3707	T12655	T12654	punct	],8
R3708	T12656	T12601	punct	.,suggested
R3709	T12658	T12659	advmod	Therefore,expressed
R3710	T12660	T12659	punct	", ",expressed
R3711	T12661	T12659	nsubj	we,expressed
R3712	T12662	T12659	advmod	also,expressed
R3713	T12663	T12664	det	the,results
R3714	T12664	T12659	dobj	results,expressed
R3715	T12665	T12666	compound	amino,acid
R3716	T12666	T12664	compound	acid,results
R3717	T12667	T12659	prep	as,expressed
R3718	T12668	T12669	compound	BCAA,alanine
R3719	T12669	T12671	compound	alanine,ratio
R3720	T12670	T12669	punct	/,alanine
R3748	T12700	T12701	punct	+,-
R3749	T12701	T12696	conj	-,+
R3750	T12702	T12701	punct	/,-
R3751	T12703	T12676	punct	.,was
R3752	T12705	T12706	det	These,results
R3753	T12706	T12710	nsubj	results,are
R3754	T12707	T12706	compound	blood,results
R3755	T12708	T12709	compound	amino,acid
R3756	T12709	T12706	compound	acid,results
R3757	T12711	T12710	acomp	consistent,are
R3758	T12712	T12711	prep	with,consistent
R3759	T12713	T12714	det	the,concentrations
R3760	T12714	T12712	pobj	concentrations,with
R3761	T12715	T12714	acl	seen,concentrations
R3762	T12716	T12715	prep	in,seen
R3763	T12717	T12716	pobj	patients,in
R3764	T12718	T12717	prep	with,patients
R3765	T12719	T12718	pobj	MSUD,with
R3766	T12720	T12721	punct	[,15
R3767	T12721	T12710	parataxis	15,are
R3768	T12722	T12721	nummod	1,15
R3769	T12723	T12721	punct	",",15
R3770	T12724	T12721	nummod	8,15
R3771	T12725	T12721	punct	",",15
R3772	T12726	T12721	punct	],15
R3773	T12727	T12710	punct	.,are
R3803	T12761	T12760	advmod	ultimately,result
R3804	T12762	T12760	prep	in,result
R3805	T12763	T12764	amod	neonatal,lethality
R3806	T12764	T12762	pobj	lethality,in
R3807	T12765	T12760	punct	.,result
R3808	T12767	T12768	det	These,phenotypes
R3809	T12768	T12769	nsubj	phenotypes,are
R3810	T12770	T12771	advmod	remarkably,similar
R3811	T12771	T12769	acomp	similar,are
R3812	T12772	T12771	prep	to,similar
R3813	T12773	T12772	pobj	that,to
R3814	T12774	T12773	acl	observed,that
R3815	T12775	T12774	prep	in,observed
R3816	T12776	T12775	pobj	humans,in
R3817	T12777	T12776	prep	with,humans
R3818	T12778	T12779	det	the,form
R3819	T12779	T12777	pobj	form,with
R3820	T12780	T12779	amod	classic,form
R3821	T12781	T12779	prep	of,form
R3822	T12782	T12781	pobj	MSUD,of
R3823	T12783	T12769	punct	.,are
R3824	T12785	T12786	advmod	Thus,model
R3825	T12787	T12786	punct	", ",model
R3826	T12788	T12789	compound	E2,mice
R3827	T12789	T12786	nsubj	mice,model
R3828	T12790	T12789	compound	knockout,mice
R3829	T12791	T12786	advmod	closely,model
R3830	T12792	T12793	amod	classic,MSUD
R3831	T12793	T12786	dobj	MSUD,model
R3832	T12794	T12786	punct	.,model
R3835	T15726	T15727	compound	Knockout,mice
R3836	T15727	T15728	nsubj	mice,model
R3837	T15729	T15727	acl	expressing,mice
R3838	T15730	T15731	det	a,transgene
R3839	T15731	T15729	dobj	transgene,expressing
R3840	T15732	T15731	amod	human,transgene
R3841	T15733	T15731	compound	E2,transgene
R3842	T15734	T15735	det	a,form
R3843	T15735	T15728	dobj	form,model
R3844	T15736	T15735	amod	variant,form
R3845	T15737	T15735	prep	of,form
R3846	T15738	T15737	pobj	MSUD,of
R3847	T15739	T15740	dep	that,mimics
R3848	T15740	T15735	relcl	mimics,form
R3849	T15741	T15742	amod	intermediate,MSUD
R3850	T15742	T15740	dobj	MSUD,mimics
R3851	T15744	T15745	aux	To,create
R3852	T15745	T15746	advcl	create,used
R3853	T15747	T15748	det	a,model
R3854	T15748	T15745	dobj	model,create
R3855	T15749	T15748	amod	murine,model
R3856	T15750	T15748	prep	of,model
R3857	T15751	T15752	det	the,form
R3858	T15752	T15750	pobj	form,of
R3859	T15753	T15752	amod	intermediate,form
R3860	T15754	T15752	compound	variant,form
R3861	T15755	T15752	prep	of,form
R3862	T15756	T15755	pobj	MSUD,of
R3863	T15757	T15746	punct	", ",used
R3864	T15758	T15746	nsubj	we,used
R3865	T15759	T15760	det	a,strategy
R3866	T15760	T15746	dobj	strategy,used
R3867	T15761	T15760	amod	transgenic,strategy
R3868	T15762	T15763	aux	to,rescue
R3869	T15763	T15746	advcl	rescue,used
R3870	T15764	T15765	det	the,elevation
R3871	T15765	T15763	dobj	elevation,rescue
R3872	T15766	T15765	amod	severe,elevation
R3873	T15767	T15765	prep	of,elevation
R3874	T15768	T15767	pobj	BCAA,of
R3875	T15769	T15765	cc	and,elevation
R3876	T15770	T15771	amod	neonatal,lethality
R3877	T15771	T15765	conj	lethality,elevation
R3878	T15772	T15773	dep	that,occurs
R3879	T15773	T15771	relcl	occurs,lethality
R3880	T15774	T15773	prep	in,occurs
R3881	T15775	T15776	det	the,model
R3882	T15776	T15774	pobj	model,in
R3883	T15777	T15776	amod	classic,model
R3884	T15778	T15776	compound	MSUD,model
R3885	T15779	T15776	compound	mouse,model
R3886	T15780	T15746	punct	.,used
R3887	T15782	T15783	poss	Our,strategy
R3888	T15783	T15784	nsubjpass	strategy,composed
R3889	T15785	T15784	auxpass	was,composed
R3890	T15786	T15784	prep	of,composed
R3891	T15787	T15788	det	a,system
R3892	T15788	T15786	pobj	system,of
R3893	T15789	T15790	nummod	two,part
R3894	T15790	T15788	nmod	part,system
R3895	T15791	T15788	amod	transgenic,system
R3896	T15792	T15793	aux	to,express
R3897	T15793	T15788	advcl	express,system
R3898	T15794	T15795	amod	human,E2
R3899	T15795	T15793	dobj	E2,express
R3900	T15796	T15793	prep	in,express
R3901	T15797	T15796	pobj	liver,in
R3902	T15798	T15793	prep	on,express
R3903	T15799	T15800	det	the,background
R3904	T15800	T15798	pobj	background,on
R3905	T15801	T15802	compound	E2,knockout
R3906	T15802	T15800	compound	knockout,background
R3907	T15803	T15784	punct	.,composed
R3908	T15805	T15806	det	This,system
R3909	T15806	T15808	nsubj	system,consisted
R3910	T15807	T15806	amod	bi-transgenic,system
R3911	T15809	T15808	prep	of,consisted
R3912	T15810	T15811	det	a,transgene
R3913	T15811	T15809	pobj	transgene,of
R3914	T15812	T15813	compound	LAP,tTA
R3915	T15813	T15811	compound	tTA,transgene
R3916	T15814	T15813	punct	-,tTA
R3917	T15815	T15811	cc	and,transgene
R3918	T15816	T15817	det	a,transgene
R3919	T15817	T15811	conj	transgene,transgene
R3920	T15818	T15819	compound	TRE,E2
R3921	T15819	T15817	compound	E2,transgene
R3922	T15820	T15819	punct	-,E2
R3923	T15821	T15822	punct	(,3A
R3924	T15822	T15808	parataxis	3A,consisted
R3925	T15823	T15822	compound	Fig.,3A
R3926	T15824	T15822	punct	),3A
R3927	T15825	T15808	punct	.,consisted
R3944	T15844	T15842	pobj	transactivator,of
R3945	T15845	T15846	npadvmod	tetracycline,controlled
R3946	T15846	T15844	amod	controlled,transactivator
R3947	T15847	T15846	punct	-,controlled
R3948	T15848	T15844	punct	(,transactivator
R3949	T15849	T15844	appos	tTA,transactivator
R3950	T15850	T15844	punct	),transactivator
R3951	T15851	T15844	punct	", ",transactivator
R3952	T15852	T15853	det	a,factor
R3953	T15853	T15844	appos	factor,transactivator
R3954	T15854	T15853	compound	transcription,factor
R3955	T15855	T15856	dep	that,stimulates
R3956	T15856	T15853	relcl	stimulates,factor
R3957	T15857	T15856	dobj	expression,stimulates
R3958	T15858	T15857	prep	of,expression
R3959	T15859	T15858	pobj	promoters,of
R3960	T15860	T15861	dep	that,harbor
R3961	T15861	T15859	relcl	harbor,promoters
R3962	T15862	T15863	det	a,element
R3963	T15863	T15861	dobj	element,harbor
R3964	T15864	T15863	compound	transactivator,element
R3965	T15865	T15863	compound	response,element
R3966	T15866	T15863	punct	(,element
R3967	T15867	T15863	appos	TRE,element
R3968	T15868	T15832	punct	),directs
R3969	T15869	T15832	punct	.,directs
R3970	T15871	T15872	det	The,transgene
R3971	T15872	T15876	nsubjpass	transgene,designed
R3972	T15873	T15874	compound	TRE,E2
R3973	T15874	T15872	compound	E2,transgene
R3974	T15875	T15874	punct	-,E2
R3975	T15877	T15876	auxpass	was,designed
R3976	T15878	T15879	aux	to,express
R3977	T15879	T15876	advcl	express,designed
R3978	T15880	T15881	det	a,cDNA
R3979	T15881	T15879	dobj	cDNA,express
R3980	T15882	T15881	amod	human,cDNA
R3981	T15883	T15881	compound	E2,cDNA
R3982	T15884	T15879	prep	from,express
R3983	T15885	T15886	det	a,TRE
R3984	T15886	T15884	pobj	TRE,from
R3985	T15887	T15886	acl	containing,TRE
R3986	T15888	T15889	amod	minimal,promoter
R3987	T15889	T15887	dobj	promoter,containing
R3988	T15890	T15879	prep	upon,express
R3989	T15891	T15890	pobj	stimulation,upon
R3990	T15892	T15891	prep	by,stimulation
R3991	T15893	T15892	pobj	tTA,by
R3992	T15894	T15876	punct	.,designed
R4008	T15941	T15942	punct	[,34
R4009	T15942	T15936	parataxis	34,zed
R4010	T15943	T15942	punct	],34
R4011	T15946	T15947	amod	Pronuclear,microinjection
R4012	T15947	T15948	nsubjpass	microinjection,used
R4013	T15949	T15948	auxpass	was,used
R4014	T15950	T15951	aux	to,produce
R4015	T15951	T15948	advcl	produce,used
R4016	T15952	T15953	amod	transgenic,mice
R4017	T15953	T15951	dobj	mice,produce
R4018	T15954	T15955	dep	that,harbored
R4019	T15955	T15953	relcl	harbored,mice
R4020	T15956	T15957	det	the,transgene
R4021	T15957	T15955	dobj	transgene,harbored
R4022	T15958	T15959	compound	TRE,E2
R4023	T15959	T15957	compound	E2,transgene
R4024	T15960	T15959	punct	-,E2
R4025	T15961	T15948	punct	.,used
R4026	T15963	T15964	prep	From,identified
R4027	T15965	T15963	pobj	injections,From
R4028	T15966	T15965	prep	at,injections
R4029	T15967	T15968	det	the,University
R4030	T15968	T15966	pobj	University,at
R4031	T15969	T15968	prep	of,University
R4032	T15970	T15969	pobj	Pittsburgh,of
R4033	T15971	T15964	punct	", ",identified
R4034	T15972	T15973	nummod	2,founders
R4035	T15973	T15964	nsubjpass	founders,identified
R4036	T15974	T15975	npadvmod	transgene,positive
R4037	T15975	T15973	amod	positive,founders
R4038	T15976	T15964	auxpass	were,identified
R4039	T15977	T15978	dep	that,led
R4040	T15978	T15964	ccomp	led,identified
R4041	T15979	T15978	prep	to,led
R4042	T15980	T15981	det	the,generation
R4043	T15981	T15979	pobj	generation,to
R4044	T15982	T15981	prep	of,generation
R4045	T15983	T15984	nummod	3,lines
R4046	T15984	T15982	pobj	lines,of
R4047	T15985	T15984	amod	different,lines
R4048	T15986	T15984	amod	transgenic,lines
R4049	T15987	T15988	punct	(,A
R4050	T15988	T15984	parataxis	A,lines
R4051	T15989	T15988	compound	Lines,A
R4052	T15990	T15988	punct	", ",A
R4053	T15991	T15988	conj	B,A
R4054	T15992	T15991	punct	", ",B
R4055	T15993	T15991	cc	&,B
R4056	T15994	T15991	conj	D,B
R4057	T15995	T15988	punct	),A
R4058	T15996	T15964	punct	.,identified
R4059	T15998	T15999	prep	From,obtained
R4060	T16000	T16001	det	the,injections
R4061	T16001	T15998	pobj	injections,From
R4062	T16002	T16001	prep	at,injections
R4063	T16003	T16004	det	the,University
R4064	T16004	T16002	pobj	University,at
R4065	T16005	T16004	prep	of,University
R4066	T16006	T16005	pobj	Cincinnati,of
R4067	T16007	T15999	punct	", ",obtained
R4068	T16008	T15999	nsubj	we,obtained
R4069	T16009	T16010	nummod	8,founders
R4070	T16010	T15999	dobj	founders,obtained
R4071	T16011	T16012	npadvmod	transgene,positive
R4072	T16012	T16010	amod	positive,founders
R4073	T16013	T15999	punct	.,obtained
R4074	T16015	T16016	amod	Subsequent,breeding
R4075	T16016	T16017	nsubj	breeding,revealed
R4076	T16018	T16016	prep	of,breeding
R4077	T16019	T16020	det	these,founders
R4078	T16020	T16018	pobj	founders,of
R4079	T16021	T16022	mark	that,had
R4080	T16022	T16017	ccomp	had,revealed
R4081	T16023	T16022	nsubj	many,had
R4082	T16024	T16023	prep	of,many
R4083	T16025	T16026	det	the,animals
R4084	T16026	T16024	pobj	animals,of
R4085	T16027	T16028	amod	multiple,sites
R4086	T16028	T16022	dobj	sites,had
R4087	T16029	T16028	compound	transgene,sites
R4088	T16030	T16028	compound	insertion,sites
R4089	T16031	T16032	dep	that,segregated
R4090	T16032	T16028	relcl	segregated,sites
R4091	T16033	T16017	punct	.,revealed
R4092	T16035	T16036	nsubj	We,established
R4093	T16037	T16038	det	a,total
R4094	T16038	T16036	dobj	total,established
R4095	T16039	T16038	prep	of,total
R4096	T16040	T16041	nummod	15,lines
R4097	T16041	T16039	pobj	lines,of
R4098	T16042	T16041	amod	different,lines
R4099	T16043	T16041	amod	transgenic,lines
R4100	T16044	T16036	prep	from,established
R4101	T16045	T16046	det	these,founders
R4102	T16046	T16044	pobj	founders,from
R4103	T16047	T16036	punct	.,established
R4104	T16049	T16050	amod	Limited,resources
R4105	T16050	T16051	nsubj	resources,allowed
R4106	T16052	T16053	nsubj	us,focus
R4107	T16053	T16051	ccomp	focus,allowed
R4108	T16054	T16053	aux	to,focus
R4109	T16055	T16053	advmod	only,focus
R4110	T16056	T16053	prep	on,focus
R4111	T16057	T16058	det	a,total
R4112	T16058	T16056	pobj	total,on
R4113	T16059	T16058	prep	of,total
R4114	T16060	T16059	pobj	9,of
R4115	T16061	T16060	prep	of,9
R4116	T16062	T16063	det	these,lines
R4117	T16063	T16061	pobj	lines,of
R4118	T16064	T16051	punct	.,allowed
R4119	T16066	T16067	det	These,lines
R4120	T16067	T16068	nsubj	lines,differed
R4121	T16069	T16068	advmod	substantially,differed
R4122	T16070	T16068	prep	in,differed
R4123	T16071	T16072	compound	transgene,number
R4124	T16072	T16070	pobj	number,in
R4125	T16073	T16072	compound	copy,number
R4126	T16074	T16075	mark	as,compared
R4127	T16075	T16068	advcl	compared,differed
R4128	T16076	T16075	prep	by,compared
R4129	T16077	T16078	compound	Southern,blot
R4130	T16078	T16079	compound	blot,analysis
R4131	T16079	T16076	pobj	analysis,by
R4132	T16080	T16079	prep	of,analysis
R4133	T16081	T16082	compound	tail,DNA
R4134	T16082	T16080	pobj	DNA,of
R4135	T16083	T16084	punct	(,shown
R4136	T16084	T16068	parataxis	shown,differed
R4137	T16085	T16084	nsubj	data,shown
R4138	T16086	T16084	neg	not,shown
R4139	T16087	T16084	punct	),shown
R4140	T16088	T16068	punct	.,differed
R4141	T16090	T16091	amod	Transgenic,mice
R4142	T16091	T16095	nsubjpass	mice,crossed
R4143	T16092	T16093	compound	TRE,E2
R4144	T16093	T16091	compound	E2,mice
R4145	T16094	T16093	punct	-,E2
R4146	T16096	T16091	prep	from,mice
R4147	T16097	T16098	det	each,line
R4148	T16098	T16096	pobj	line,from
R4149	T16099	T16100	punct	(,offspring
R4150	T16100	T16098	parataxis	offspring,line
R4151	T16101	T16100	preconj	either,offspring
R4152	T16102	T16100	nmod	founders,offspring
R4153	T16103	T16102	cc	or,founders
R4154	T16104	T16102	conj	F1,founders
R4155	T16105	T16100	punct	),offspring
R4156	T16106	T16095	auxpass	were,crossed
R4157	T16107	T16095	prep	to,crossed
R4158	T16108	T16107	pobj	mice,to
R4159	T16109	T16110	dep	that,were
R4160	T16110	T16108	relcl	were,mice
R4161	T16111	T16110	acomp	positive,were
R4162	T16112	T16111	prep	for,positive
R4163	T16113	T16114	det	the,transgene
R4164	T16114	T16112	pobj	transgene,for
R4165	T16115	T16116	compound	LAP,tTA
R4166	T16116	T16114	compound	tTA,transgene
R4167	T16117	T16116	punct	-,tTA
R4168	T16118	T16110	cc	and,were
R4169	T16119	T16110	conj	were,were
R4170	T16120	T16119	acomp	heterozygous,were
R4171	T16121	T16120	prep	for,heterozygous
R4172	T16122	T16123	det	the,knockout
R4173	T16123	T16121	pobj	knockout,for
R4174	T16124	T16123	compound	E2,knockout
R4175	T16125	T16095	punct	.,crossed
R4176	T16127	T16128	advmod	Ultimately,used
R4177	T16129	T16128	punct	", ",used
R4178	T16130	T16131	amod	breeding,pairs
R4179	T16131	T16128	nsubjpass	pairs,used
R4180	T16132	T16128	auxpass	were,used
R4181	T16133	T16134	prep	in,were
R4182	T16134	T16128	ccomp	were,used
R4183	T16135	T16133	pobj	which,in
R4184	T16136	T16137	det	the,mice
R4185	T16137	T16134	nsubj	mice,were
R4186	T16138	T16134	acomp	heterozygous,were
R4187	T16139	T16138	prep	for,heterozygous
R4188	T16140	T16141	det	the,transgene
R4189	T16141	T16139	pobj	transgene,for
R4190	T16142	T16143	compound	LAP,tTA
R4191	T16143	T16141	compound	tTA,transgene
R4192	T16144	T16143	punct	-,tTA
R4193	T16145	T16141	punct	", ",transgene
R4194	T16146	T16147	det	the,transgene
R4195	T16147	T16141	conj	transgene,transgene
R4196	T16148	T16149	compound	TRE,E2
R4197	T16149	T16147	compound	E2,transgene
R4198	T16150	T16149	punct	-,E2
R4199	T16151	T16147	punct	", ",transgene
R4200	T16152	T16147	cc	and,transgene
R4201	T16153	T16154	det	the,knockout
R4202	T16154	T16147	conj	knockout,transgene
R4203	T16155	T16154	compound	E2,knockout
R4204	T16156	T16128	punct	.,used
R4205	T16158	T16159	aux	To,screen
R4206	T16159	T16161	advcl	screen,genotyped
R4207	T16160	T16159	advmod	efficiently,screen
R4208	T16162	T16159	prep	for,screen
R4209	T16163	T16164	det	the,ability
R4210	T16164	T16162	pobj	ability,for
R4211	T16165	T16164	prep	of,ability
R4212	T16166	T16167	det	the,transgenes
R4213	T16167	T16165	pobj	transgenes,of
R4214	T16168	T16169	aux	to,rescue
R4215	T16169	T16164	acl	rescue,ability
R4216	T16170	T16171	det	the,knockout
R4217	T16171	T16169	dobj	knockout,rescue
R4218	T16172	T16169	prep	from,rescue
R4219	T16173	T16174	amod	neonatal,lethality
R4220	T16174	T16172	pobj	lethality,from
R4221	T16175	T16161	punct	", ",genotyped
R4222	T16176	T16161	nsubj	we,genotyped
R4223	T16177	T16161	dobj	litters,genotyped
R4224	T16178	T16161	prep	at,genotyped
R4225	T16179	T16178	pobj	weaning,at
R4226	T16180	T16161	punct	.,genotyped
R4227	T16182	T16183	det	This,strategy
R4228	T16183	T16185	nsubjpass	strategy,expected
R4229	T16184	T16183	amod	breeding,strategy
R4230	T16186	T16185	auxpass	is,expected
R4231	T16187	T16188	aux	to,result
R4232	T16188	T16185	xcomp	result,expected
R4233	T16189	T16188	prep	in,result
R4234	T16190	T16191	det	a,maximum
R4235	T16191	T16189	pobj	maximum,in
R4236	T16192	T16191	amod	theoretical,maximum
R4237	T16193	T16191	prep	of,maximum
R4238	T16194	T16193	pobj	animals,of
R4239	T16195	T16194	prep	with,animals
R4240	T16196	T16197	det	the,genotype
R4241	T16197	T16195	pobj	genotype,with
R4242	T16198	T16197	compound	rescue,genotype
R4243	T16199	T16200	punct	(,knockout
R4244	T16200	T16197	parataxis	knockout,genotype
R4245	T16201	T16200	advmod	i.e.,knockout
R4246	T16202	T16200	punct	", ",knockout
R4247	T16203	T16200	amod	homozygous,knockout
R4248	T16204	T16200	compound	E2,knockout
R4249	T16205	T16200	cc	and,knockout
R4250	T16206	T16200	conj	positive,knockout
R4251	T16207	T16206	prep	for,positive
R4252	T16208	T16209	det	both,transgenes
R4253	T16209	T16207	pobj	transgenes,for
R4254	T16210	T16200	punct	),knockout
R4255	T16211	T16191	prep	of,maximum
R4256	T16212	T16213	nummod	14,%
R4257	T16213	T16211	pobj	%,of
R4258	T16214	T16185	punct	.,expected
R4259	T16216	T16217	compound	Fig.,4A
R4260	T16217	T16218	nsubj	4A,shows
R4261	T16219	T16220	det	the,percentage
R4262	T16220	T16218	dobj	percentage,shows
R4263	T16221	T16220	prep	of,percentage
R4264	T16222	T16221	pobj	mice,of
R4265	T16223	T16222	amod	alive,mice
R4266	T16224	T16223	prep	at,alive
R4267	T16225	T16224	pobj	weaning,at
R4268	T16226	T16220	prep	with,percentage
R4269	T16227	T16228	det	the,genotype
R4270	T16228	T16226	pobj	genotype,with
R4271	T16229	T16228	compound	rescue,genotype
R4272	T16230	T16228	prep	from,genotype
R4273	T16231	T16232	det	each,line
R4274	T16232	T16230	pobj	line,from
R4275	T16279	T16280	advmod	Thus,appears
R4276	T16233	T16232	amod	transgenic,line
R4277	T16281	T16280	punct	", ",appears
R4278	T16234	T16232	acl	tested,line
R4279	T16282	T16283	det	the,transgene
R4280	T16283	T16280	nsubj	transgene,appears
R4281	T16235	T16218	punct	.,shows
R4282	T16284	T16283	compound	525A,transgene
R4283	T16285	T16286	aux	to,be
R4284	T16237	T16238	amod	Considerable,variability
R4285	T16286	T16280	xcomp	be,appears
R4286	T16287	T16288	advmod	highly,effective
R4287	T16288	T16286	acomp	effective,be
R4288	T16289	T16288	prep	at,effective
R4289	T16238	T16239	nsubjpass	variability,observed
R4290	T16240	T16239	auxpass	was,observed
R4293	T16241	T16239	prep	between,observed
R4297	T16242	T16241	pobj	lines,between
R4302	T16243	T16239	punct	.,observed
R4305	T16245	T16246	det	The,line
R4309	T16246	T16247	nsubj	line,was
R4311	T16248	T16246	prep	with,line
R4316	T16249	T16250	det	the,percentage
R4318	T16250	T16248	pobj	percentage,with
R4322	T16251	T16250	amod	highest,percentage
R4326	T16252	T16250	prep	of,percentage
R4328	T16253	T16254	compound	rescue,mice
R4332	T16254	T16252	pobj	mice,of
R4336	T16255	T16250	prep	at,percentage
R4340	T16256	T16255	pobj	weaning,at
R4344	T16257	T16258	nmod	line,A
R4345	T16333	T16334	amod	weaned,pups
R4346	T16334	T16332	pobj	pups,of
R4347	T16335	T16319	punct	.,produced
R4348	T16258	T16247	attr	A,was
R4349	T16259	T16258	nummod	525,A
R4350	T16260	T16247	punct	.,was
R4351	T16262	T16263	advmod	Approximately,10
R4352	T16263	T16264	nummod	10,%
R4354	T16264	T16265	nsubj	%,had
R4355	T16266	T16264	prep	of,%
R4356	T16267	T16268	amod	weaned,pups
R4357	T16268	T16266	pobj	pups,of
R4358	T16269	T16268	punct	", ",pups
R4359	T16270	T16268	amod	surviving,pups
R4362	T16271	T16264	prep	from,%
R4363	T16272	T16273	det	this,line
R4364	T16273	T16271	pobj	line,from
R4365	T16274	T16275	det	the,genotype
R4366	T16275	T16265	dobj	genotype,had
R4367	T16276	T16275	compound	rescue,genotype
R4368	T16277	T16265	punct	.,had
R4406	T16399	T16382	punct	.,shows
R4410	T16401	T16402	det	The,values
R4412	T16402	T16406	nsubj	values,were
R4413	T16403	T16404	compound	BCAA,alanine
R4414	T16404	T16402	compound	alanine,values
R4416	T16405	T16404	punct	/,alanine
R4420	T16407	T16402	prep	for,values
R4424	T16408	T16407	pobj	mice,for
R4428	T16409	T16408	prep	from,mice
R4432	T16410	T16411	det	both,lines
R4436	T16411	T16409	pobj	lines,from
R4439	T16521	T16520	compound	E2,protein
R4440	T16412	T16413	advmod	significantly,elevated
R4441	T16522	T16514	prep	in,activity
R4442	T16523	T16522	pobj	liver,in
R4443	T16524	T16523	prep	of,liver
R4444	T16413	T16406	acomp	elevated,were
R4445	T16525	T16526	compound	Lines,A
R4446	T16526	T16524	pobj	A,of
R4447	T16527	T16526	cc	and,A
R4448	T16414	T16406	prep	compared,were
R4449	T16528	T16526	conj	525A,A
R4450	T16529	T16503	auxpass	was,examined
R4451	T16415	T16414	prep	to,compared
R4452	T16530	T16503	punct	.,examined
R4453	T16416	T16415	pobj	controls,to
R4454	T16417	T16416	prep	for,controls
R4455	T16532	T16533	nmod	BCKDH,activity
R4456	T16533	T16535	nsubj	activity,was
R4457	T16534	T16533	amod	enzymatic,activity
R4458	T16418	T16417	pobj	all,for
R4459	T16536	T16533	prep	in,activity
R4460	T16537	T16536	pobj	liver,in
R4461	T16538	T16537	prep	from,liver
R4462	T16539	T16540	compound	Lines,A
R4463	T16419	T16418	prep	of,all
R4464	T16540	T16538	pobj	A,from
R4465	T16541	T16540	cc	and,A
R4466	T16420	T16421	det	the,points
R4467	T16542	T16540	conj	525A,A
R4468	T16543	T16544	punct	~,6
R4469	T16544	T16545	nummod	6,%
R4470	T16421	T16419	pobj	points,of
R4471	T16545	T16535	attr	%,was
R4472	T16546	T16544	cc	and,6
R4473	T16422	T16421	compound	time,points
R4474	T16547	T16544	conj	5,6
R4475	T16548	T16535	punct	", ",was
R4476	T16549	T16535	advmod	respectively,was
R4477	T16423	T16421	acl	analyzed,points
R4478	T16550	T16535	punct	", ",was
R4479	T16551	T16535	prep	of,was
R4480	T16552	T16553	det	the,activity
R4481	T16424	T16406	punct	.,were
R4482	T16553	T16551	pobj	activity,of
R4483	T16554	T16553	amod	enzymatic,activity
R4484	T16555	T16553	amod	present,activity
R4485	T16427	T16428	mark	that,are
R4486	T16556	T16555	prep	in,present
R4487	T16557	T16558	compound	control,liver
R4488	T16558	T16556	pobj	liver,in
R4489	T16559	T16560	punct	(,4C
R4490	T16560	T16535	parataxis	4C,was
R4491	T16428	T16426	ccomp	are,Note
R4492	T16561	T16560	compound	Fig.,4C
R4493	T16562	T16560	punct	),4C
R4494	T16563	T16535	punct	.,was
R4495	T16429	T16430	det	the,values
R4496	T16565	T16566	mark	As,shown
R4497	T16430	T16428	nsubj	values,are
R4498	T16566	T16567	advcl	shown,was
R4499	T16568	T16566	prep	in,shown
R4500	T16431	T16432	compound	BCAA,alanine
R4501	T16569	T16570	compound	Fig.,3B
R4502	T16570	T16568	pobj	3B,in
R4503	T16571	T16567	punct	", ",was
R4504	T16432	T16430	compound	alanine,values
R4505	T16572	T16573	det	the,amount
R4506	T16573	T16567	nsubj	amount,was
R4507	T16574	T16573	prep	of,amount
R4508	T16575	T16576	amod	human,E2
R4509	T16576	T16574	pobj	E2,of
R4510	T16433	T16432	punct	/,alanine
R4511	T16577	T16578	punct	(,Kd
R4512	T16578	T16576	parataxis	Kd,E2
R4513	T16579	T16580	amod	predicted,MW
R4514	T16580	T16578	nsubj	MW,Kd
R4515	T16434	T16430	prep	for,values
R4516	T16581	T16582	punct	~,54
R4517	T16582	T16578	nummod	54,Kd
R4518	T16583	T16578	punct	),Kd
R4519	T16435	T16436	det	the,mice
R4520	T16584	T16573	prep	in,amount
R4521	T16585	T16586	det	these,mice
R4522	T16586	T16584	pobj	mice,in
R4523	T16436	T16434	pobj	mice,for
R4524	T16587	T16586	amod	transgenic,mice
R4525	T16588	T16589	advmod	quite,variable
R4526	T16589	T16567	acomp	variable,was
R4527	T16437	T16436	amod	transgenic,mice
R4528	T16590	T16589	prep	between,variable
R4529	T16438	T16428	acomp	intermediate,are
R4530	T16439	T16428	prep	between,are
R4531	T16591	T16590	pobj	mice,between
R4532	T16592	T16567	punct	.,was
R4534	T16594	T16595	prep	In,was
R4535	T16596	T16594	pobj	some,In
R4536	T16597	T16596	prep	of,some
R4538	T16598	T16599	det	these,mice
R4539	T16599	T16597	pobj	mice,of
R4541	T16600	T16599	amod	transgenic,mice
R4542	T16601	T16595	punct	", ",was
R4543	T16602	T16603	det	the,level
R4545	T16603	T16595	nsubj	level,was
R4546	T16604	T16603	prep	of,level
R4547	T16605	T16606	amod	human,E2
R4549	T16606	T16604	pobj	E2,of
R4550	T16607	T16608	advmod	approximately,equal
R4554	T16608	T16595	acomp	equal,was
R4555	T16609	T16608	prep	to,equal
R4557	T16610	T16611	det	the,amount
R4558	T16611	T16609	pobj	amount,to
R4559	T16612	T16611	prep	of,amount
R4560	T16613	T16614	compound	mouse,E2
R4561	T16614	T16612	pobj	E2,of
R4562	T16615	T16616	punct	(,Kd
R4564	T16616	T16614	parataxis	Kd,E2
R4566	T16617	T16618	punct	~,47
R4567	T16618	T16616	nummod	47,Kd
R4569	T16619	T16616	punct	),Kd
R4570	T16620	T16611	acl	produced,amount
R4571	T16621	T16620	prep	in,produced
R4572	T16622	T16621	pobj	liver,in
R4573	T16623	T16622	prep	of,liver
R4575	T16624	T16625	amod	nontransgenic,mice
R4576	T16625	T16623	pobj	mice,of
R4578	T16626	T16625	compound	control,mice
R4579	T16627	T16595	punct	.,was
R4581	T16629	T16630	det	The,observations
R4582	T16630	T16631	nsubj	observations,suggest
R4584	T16632	T16633	mark	that,result
R4585	T16633	T16630	acl	result,observations
R4587	T16634	T16635	det	these,amounts
R4588	T16635	T16633	nsubj	amounts,result
R4589	T16636	T16637	advmod	near,normal
R4591	T16637	T16635	amod	normal,amounts
R4592	T16638	T16635	prep	of,amounts
R4594	T16639	T16640	compound	E2,protein
R4595	T16640	T16638	pobj	protein,of
R4596	T16641	T16633	prep	in,result
R4598	T16642	T16643	advmod	only,6
R4599	T16643	T16647	nummod	6,%
R4600	T16644	T16643	punct	~,6
R4602	T16645	T16643	quantmod	5,6
R4603	T16646	T16643	punct	–,6
R4604	T16647	T16641	pobj	%,in
R4606	T16648	T16647	prep	of,%
R4607	T16649	T16650	amod	normal,activity
R4608	T16650	T16648	pobj	activity,of
R4610	T16651	T16650	compound	BCKDH,activity
R4611	T16652	T16650	compound	enzyme,activity
R4612	T16653	T16654	mark	that,functioning
R4613	T16654	T16631	ccomp	functioning,suggest
R4614	T16655	T16656	nmod	transgene,E2
R4615	T16656	T16654	nsubj	E2,functioning
R4617	T16657	T16656	amod	derived,E2
R4618	T16658	T16654	aux	is,functioning
R4619	T16659	T16654	prep	at,functioning
R4621	T16660	T16661	det	a,level
R4622	T16661	T16659	pobj	level,at
R4624	T16662	T16661	amod	suboptimal,level
R4625	T16663	T16631	punct	.,suggest
R4627	T16665	T16666	nsubj	It,is
R4628	T16667	T16666	acomp	probable,is
R4630	T16668	T16669	mark	that,interfered
R4631	T16669	T16666	ccomp	interfered,is
R4632	T16670	T16671	det	the,tag
R4634	T16671	T16669	nsubj	tag,interfered
R4635	T16672	T16673	compound	c,myc
R4637	T16673	T16671	compound	myc,tag
R4638	T16674	T16673	punct	-,myc
R4639	T16675	T16671	prep	at,tag
R4641	T16676	T16677	det	the,terminus
R4642	T16677	T16675	pobj	terminus,at
R4643	T16678	T16677	compound	carboxy,terminus
R4645	T16679	T16677	prep	of,terminus
R4646	T16680	T16681	det	the,E2
R4647	T16681	T16679	pobj	E2,of
R4649	T16682	T16683	npadvmod	transgene,derived
R4650	T16683	T16681	amod	derived,E2
R4651	T16684	T16681	amod	human,E2
R4653	T16685	T16669	prep	with,interfered
R4654	T16686	T16687	amod	enzymatic,activity
R4655	T16687	T16685	pobj	activity,with
R4657	T16688	T16666	punct	.,is
R4659	T16690	T16691	det	This,interpretation
R4660	T16691	T16692	nsubj	interpretation,is
R4662	T16693	T16692	acomp	consistent,is
R4663	T16694	T16693	prep	with,consistent
R4664	T16695	T16696	amod	previous,studies
R4665	T16696	T16694	pobj	studies,with
R4666	T16697	T16698	dep	which,revealed
R4668	T16698	T16696	relcl	revealed,studies
R4669	T16699	T16698	aux	have,revealed
R4670	T16700	T16701	mark	that,is
R4673	T16701	T16698	ccomp	is,revealed
R4674	T16702	T16703	det	the,terminus
R4675	T16714	T16701	acomp	essential,is
R4676	T16703	T16701	nsubj	terminus,is
R4677	T16704	T16703	compound	carboxy,terminus
R4678	T16705	T16703	prep	of,terminus
R4679	T16706	T16707	det	an,subunit
R4680	T16707	T16705	pobj	subunit,of
R4681	T16708	T16707	amod	analogous,subunit
R4682	T16715	T16714	prep	for,essential
R4683	T16709	T16707	prep	of,subunit
R4684	T16710	T16711	det	the,complex
R4685	T16711	T16709	pobj	complex,of
R4686	T16716	T16717	compound	subunit,interactions
R4687	T16712	T16711	compound	pyruvate,complex
R4688	T16713	T16711	compound	dehydrogenase,complex
R4689	T16717	T16715	pobj	interactions,for
R4690	T16718	T16719	punct	[,35
R4691	T16719	T16701	parataxis	35,is
R4692	T16820	T16819	pobj	E2,of
R4693	T16821	T16808	acomp	negligible,is
R4694	T16720	T16719	punct	],35
R4695	T16822	T16808	prep	in,is
R4696	T16823	T16824	det	those,tissues
R4697	T16824	T16822	pobj	tissues,in
R4698	T16721	T16701	cc	and,is
R4699	T16825	T16808	punct	.,is
R4700	T16722	T16723	nsubj	insertion,interfered
R4701	T16827	T16828	amod	Long,term
R4702	T16828	T16830	compound	term,survival
R4703	T16829	T16828	punct	-,term
R4704	T16723	T16701	conj	interfered,is
R4705	T16830	T16831	nsubjpass	survival,plotted
R4706	T16724	T16722	prep	of,insertion
R4707	T16832	T16830	prep	of,survival
R4708	T16833	T16834	det	the,mice
R4709	T16834	T16832	pobj	mice,of
R4710	T16725	T16726	det	a,tag
R4711	T16835	T16834	prep	from,mice
R4712	T16836	T16837	det	these,lines
R4713	T16837	T16835	pobj	lines,from
R4714	T16838	T16837	nummod	two,lines
R4715	T16839	T16837	amod	transgenic,lines
R4716	T16840	T16841	dep	that,survived
R4717	T16726	T16724	pobj	tag,of
R4718	T16841	T16837	relcl	survived,lines
R4719	T16842	T16841	prep	beyond,survived
R4720	T16843	T16842	pobj	weaning,beyond
R4721	T16727	T16726	compound	Hisx6,tag
R4722	T16844	T16831	auxpass	is,plotted
R4723	T16845	T16831	prep	in,plotted
R4724	T16846	T16847	compound	Fig.,4D
R4725	T16728	T16722	prep	on,insertion
R4726	T16847	T16845	pobj	4D,in
R4727	T16848	T16831	punct	.,plotted
R4728	T16729	T16730	det	the,terminus
R4729	T16850	T16851	mark	Although,collected
R4730	T16730	T16728	pobj	terminus,on
R4731	T16851	T16859	advcl	collected,is
R4732	T16852	T16853	compound	survival,data
R4733	T16853	T16851	nsubjpass	data,collected
R4734	T16854	T16853	prep	for,data
R4735	T16731	T16730	compound	carboxy,terminus
R4736	T16855	T16856	compound	control,animals
R4737	T16856	T16854	pobj	animals,for
R4738	T16857	T16851	auxpass	was,collected
R4739	T16732	T16730	prep	of,terminus
R4740	T16858	T16851	neg	not,collected
R4741	T16860	T16859	punct	", ",is
R4742	T16733	T16734	det	an,subunit
R4743	T16861	T16859	nsubj	it,is
R4744	T16862	T16863	advmod	readily,apparent
R4745	T16734	T16732	pobj	subunit,of
R4746	T16863	T16859	acomp	apparent,is
R4747	T16864	T16865	mark	that,compromised
R4748	T16865	T16859	ccomp	compromised,is
R4749	T16735	T16734	amod	analogous,subunit
R4750	T16866	T16865	nsubjpass	survival,compromised
R4751	T16867	T16866	prep	of,survival
R4752	T16868	T16869	det	the,mice
R4753	T16736	T16737	nmod	E.,coli
R4754	T16869	T16867	pobj	mice,of
R4755	T16870	T16869	compound	rescue,mice
R4756	T16871	T16865	auxpass	was,compromised
R4757	T16737	T16734	nmod	coli,subunit
R4758	T16872	T16859	punct	.,is
R4759	T16874	T16875	prep	By,were
R4760	T16738	T16723	prep	with,interfered
R4761	T16876	T16877	nummod	16,weeks
R4762	T16739	T16740	amod	normal,assembly
R4763	T16877	T16874	pobj	weeks,By
R4764	T16878	T16875	punct	", ",were
R4765	T16879	T16880	det	all,mice
R4766	T16740	T16738	pobj	assembly,with
R4767	T16880	T16875	nsubj	mice,were
R4768	T16881	T16880	prep	of,mice
R4769	T16741	T16740	compound	subunit,assembly
R4770	T16882	T16883	compound	Line,A
R4771	T16883	T16881	pobj	A,of
R4772	T16884	T16875	acomp	moribund,were
R4773	T16742	T16743	punct	[,36
R4774	T16885	T16884	cc	and,moribund
R4775	T16886	T16887	advmod	humanely,sacrificed
R4776	T16887	T16884	conj	sacrificed,moribund
R4777	T16743	T16723	parataxis	36,interfered
R4778	T16888	T16875	punct	", ",were
R4779	T16889	T16875	cc	or,were
R4780	T16744	T16743	punct	],36
R4781	T16890	T16891	auxpass	were,found
R4782	T16891	T16875	conj	found,were
R4783	T16892	T16891	advcl	dead,found
R4784	T16745	T16692	punct	.,is
R4785	T16893	T16891	prep	in,found
R4786	T16894	T16895	poss	their,cage
R4787	T16747	T16748	nsubj	It,is
R4788	T16895	T16893	pobj	cage,in
R4789	T16896	T16875	punct	.,were
R4790	T16898	T16899	nsubj	Survival,appeared
R4791	T16749	T16748	advmod	also,is
R4792	T16900	T16898	prep	of,Survival
R4793	T16901	T16902	compound	Line,525A
R4794	T16902	T16900	pobj	525A,of
R4795	T16750	T16748	acomp	possible,is
R4796	T16903	T16904	aux	to,be
R4797	T16904	T16899	xcomp	be,appeared
R4798	T16905	T16906	advmod	somewhat,better
R4799	T16751	T16752	mark	that,was
R4800	T16906	T16904	acomp	better,be
R4801	T16907	T16899	punct	.,appeared
R4802	T16752	T16748	ccomp	was,is
R4803	T16909	T16910	prep	At,were
R4804	T16753	T16754	amod	human,E2
R4805	T16911	T16912	nummod	20,weeks
R4806	T16912	T16909	pobj	weeks,At
R4807	T16913	T16912	prep	of,weeks
R4808	T16754	T16752	nsubj	E2,was
R4809	T16914	T16913	pobj	age,of
R4810	T16915	T16910	punct	", ",were
R4811	T16916	T16917	punct	~,12
R4812	T16755	T16752	neg	not,was
R4813	T16917	T16918	nummod	12,%
R4814	T16918	T16910	nsubj	%,were
R4815	T16919	T16918	prep	of,%
R4816	T16756	T16757	advmod	fully,functional
R4817	T16920	T16919	pobj	mice,of
R4818	T16921	T16922	punct	(,2
R4819	T16922	T16920	parataxis	2,mice
R4820	T16923	T16922	prep	of,2
R4821	T16924	T16923	pobj	16,of
R4822	T16757	T16752	acomp	functional,was
R4823	T16925	T16922	punct	),2
R4824	T16758	T16759	advmod	when,complexed
R4825	T16759	T16752	advcl	complexed,was
R4826	T16760	T16759	prep	with,complexed
R4827	T16926	T16910	advmod	still,were
R4828	T16927	T16910	acomp	alive,were
R4829	T16761	T16762	compound	mouse,E1
R4830	T16928	T16910	punct	.,were
R4831	T16762	T16760	pobj	E1,with
R4832	T16930	T16931	det	These,survivors
R4833	T16931	T16934	nsubj	survivors,died
R4834	T16932	T16931	nummod	two,survivors
R4835	T16763	T16762	cc	and,E1
R4836	T16933	T16931	amod	rare,survivors
R4837	T16764	T16765	compound	E3,subunits
R4838	T16935	T16934	prep	at,died
R4839	T16936	T16937	nummod	40,weeks
R4840	T16937	T16935	pobj	weeks,at
R4841	T16765	T16762	conj	subunits,E1
R4842	T16938	T16936	cc	and,40
R4843	T16766	T16748	punct	.,is
R4844	T16939	T16936	conj	60,40
R4845	T16940	T16937	prep	of,weeks
R4846	T16941	T16940	pobj	age,of
R4847	T16942	T16934	punct	.,died
R4848	T16768	T16769	amod	Human,E2
R4849	T16944	T16945	prep	In,had
R4850	T16946	T16944	pobj	summary,In
R4851	T16769	T16770	nsubj	E2,shares
R4852	T16947	T16945	punct	", ",had
R4853	T16948	T16949	det	these,mice
R4854	T16771	T16772	punct	~,88
R4855	T16949	T16945	nsubj	mice,had
R4856	T16950	T16949	amod	surviving,mice
R4857	T16951	T16952	compound	BCAA,alanine
R4858	T16952	T16954	compound	alanine,ratios
R4859	T16772	T16773	nummod	88,%
R4860	T16953	T16952	punct	/,alanine
R4861	T16954	T16945	dobj	ratios,had
R4862	T16955	T16956	dep	that,were
R4863	T16773	T16774	compound	%,identity
R4864	T16956	T16954	relcl	were,ratios
R4865	T16957	T16956	acomp	intermediate,were
R4866	T16958	T16957	prep	between,intermediate
R4867	T16774	T16770	dobj	identity,shares
R4868	T16959	T16958	pobj	controls,between
R4869	T16960	T16959	cc	and,controls
R4870	T16961	T16959	conj	knockouts,controls
R4871	T16962	T16945	cc	and,had
R4872	T16963	T16964	nsubj	they,expressed
R4873	T16775	T16774	prep	to,identity
R4874	T16964	T16945	conj	expressed,had
R4875	T16965	T16966	punct	~,6
R4876	T16776	T16777	compound	mouse,E2
R4877	T16777	T16775	pobj	E2,to
R4878	T16966	T16969	nummod	6,%
R4879	T16778	T16770	prep	at,shares
R4880	T16967	T16966	quantmod	5,6
R4881	T16968	T16966	punct	–,6
R4882	T16969	T16964	dobj	%,expressed
R4883	T16779	T16780	det	the,level
R4884	T16970	T16969	prep	of,%
R4885	T16971	T16972	amod	normal,activity
R4886	T16780	T16778	pobj	level,at
R4887	T16972	T16970	pobj	activity,of
R4888	T16973	T16972	compound	BCKDH,activity
R4889	T16974	T16972	compound	enzyme,activity
R4890	T16781	T16782	compound	amino,acid
R4891	T16975	T16972	prep	in,activity
R4892	T16976	T16977	det	the,liver
R4893	T16782	T16780	compound	acid,level
R4894	T16977	T16975	pobj	liver,in
R4895	T16978	T16945	punct	.,had
R4896	T16783	T16770	punct	.,shares
R4897	T16980	T16981	det	These,observations
R4898	T16785	T16786	nsubj	We,used
R4899	T16981	T16983	nsubj	observations,are
R4900	T16982	T16981	amod	phenotypic,observations
R4901	T16984	T16985	advmod	remarkably,similar
R4902	T16985	T16983	acomp	similar,are
R4903	T16787	T16786	advmod	also,used
R4904	T16986	T16985	prep	to,similar
R4905	T16987	T16988	det	the,phenotype
R4906	T16988	T16986	pobj	phenotype,to
R4907	T16788	T16789	compound	western,blot
R4908	T16789	T16790	compound	blot,analysis
R4909	T16989	T16988	amod	clinical,phenotype
R4910	T16790	T16786	dobj	analysis,used
R4911	T16990	T16988	acl	observed,phenotype
R4912	T16991	T16990	prep	in,observed
R4913	T16992	T16993	compound	MSUD,patients
R4914	T16791	T16792	aux	to,analyze
R4915	T16993	T16991	pobj	patients,in
R4916	T16994	T16993	prep	with,patients
R4917	T16995	T16996	det	the,form
R4918	T16792	T16786	advcl	analyze,used
R4919	T16996	T16994	pobj	form,with
R4920	T16997	T16996	amod	intermediate,form
R4921	T16998	T16996	prep	of,form
R4922	T16999	T17000	det	the,disease
R4923	T17000	T16998	pobj	disease,of
R4924	T17001	T17002	punct	[,1
R4925	T16793	T16792	dobj	expression,analyze
R4926	T17002	T16983	parataxis	1,are
R4927	T17003	T17002	punct	],1
R4928	T17004	T16983	punct	.,are
R4929	T16794	T16793	prep	of,expression
R4930	T17006	T17007	advmod	Thus,represent
R4931	T16795	T16796	det	the,transgene
R4932	T17008	T17007	punct	", ",represent
R4933	T17009	T17010	det	these,mice
R4934	T17010	T17007	nsubj	mice,represent
R4935	T16796	T16794	pobj	transgene,of
R4936	T17011	T17010	compound	rescue,mice
R4937	T17012	T17013	det	a,model
R4938	T17013	T17007	dobj	model,represent
R4939	T16797	T16796	compound	E2,transgene
R4940	T17014	T17015	advmod	very,useful
R4941	T17015	T17013	amod	useful,model
R4942	T16798	T16792	prep	in,analyze
R4943	T17016	T17013	prep	of,model
R4944	T17017	T17018	det	the,phenotype
R4945	T17018	T17016	pobj	phenotype,of
R4946	T16799	T16798	pobj	brain,in
R4947	T17019	T17018	amod	intermediate,phenotype
R4948	T17020	T17018	compound	MSUD,phenotype
R4949	T17021	T17007	punct	.,represent
R4950	T16800	T16799	punct	", ",brain
R4951	T16801	T16799	conj	kidney,brain
R4952	T16802	T16801	cc	and,kidney
R4953	T16803	T16801	conj	muscle,kidney
R4954	T16804	T16786	punct	.,used
R4955	T16806	T16807	mark	As,shown
R4956	T16807	T16808	advcl	shown,is
R4957	T16809	T16807	prep	in,shown
R4958	T16810	T16811	compound	Figure,3C
R4959	T16811	T16809	pobj	3C,in
R4960	T16812	T16811	punct	", ",3C
R4961	T16813	T16811	conj	D,3C
R4962	T16814	T16813	cc	and,D
R4963	T16815	T16813	conj	3E,D
R4964	T16816	T16807	advmod	respectively,shown
R4965	T16817	T16808	punct	", ",is
R4966	T16818	T16808	nsubj	expression,is
R4967	T16819	T16818	prep	of,expression
R6552	T22807	T22808	amod	Biochemical,characterization
R6553	T22809	T22808	prep	of,characterization
R6554	T22810	T22811	det	the,model
R6555	T22811	T22809	pobj	model,of
R6556	T22812	T22811	amod	classic,model
R6557	T22813	T22811	nmod	MSUD,model
R6558	T22814	T22811	amod	murine,model
R6559	T22815	T22808	punct	.,characterization
R6560	T22817	T22818	meta	A,activity
R6561	T22819	T22818	punct	", ",activity
R6562	T22820	T22818	compound	BCKDH,activity
R6563	T22821	T22818	compound	enzyme,activity
R6564	T22822	T22818	prep	in,activity
R6565	T22823	T22822	pobj	liver,in
R6566	T22824	T22823	prep	of,liver
R6567	T22825	T22826	amod	newborn,mice
R6568	T22826	T22824	pobj	mice,of
R6569	T22827	T22828	amod	wild,type
R6570	T22828	T22829	nmod	type,control
R6571	T22829	T22830	nmod	control,knockout
R6572	T22830	T22826	compound	knockout,mice
R6573	T22831	T22832	punct	(,+
R6574	T22832	T22829	punct	+,control
R6575	T22833	T22832	punct	+,+
R6576	T22834	T22832	punct	/,+
R6577	T22835	T22832	punct	),+
R6578	T22836	T22829	punct	", ",control
R6579	T22837	T22829	conj	heterozygous,control
R6580	T22838	T22839	punct	(,-
R6581	T22839	T22837	punct	-,heterozygous
R6582	T22840	T22839	punct	+,-
R6583	T22841	T22839	punct	/,-
R6584	T22842	T22839	punct	),-
R6585	T22843	T22837	punct	", ",heterozygous
R6586	T22844	T22837	cc	and,heterozygous
R6587	T22845	T22837	conj	homozygous,heterozygous
R6588	T22846	T22847	punct	(,-
R6589	T22847	T22845	punct	-,homozygous
R6590	T22848	T22847	punct	-,-
R6591	T22849	T22847	punct	/,-
R6592	T22850	T22847	punct	),-
R6593	T22851	T22818	punct	.,activity
R6594	T22853	T22854	compound	Enzyme,activity
R6595	T22854	T22855	nsubj	activity,was
R6596	T22856	T22857	advmod	significantly,reduced
R6597	T22857	T22855	acomp	reduced,was
R6598	T22858	T22855	prep	in,was
R6599	T22859	T22860	punct	+,-
R6600	T22860	T22862	punct	-,liver
R6601	T22861	T22860	punct	/,-
R6602	T22862	T22858	pobj	liver,in
R6603	T22863	T22855	prep	compared,was
R6604	T22864	T22863	prep	to,compared
R6605	T22865	T22866	punct	+,+
R6606	T22866	T22864	punct	+,to
R6607	T22867	T22866	punct	/,+
R6608	T22868	T22855	punct	", ",was
R6609	T22869	T22855	cc	and,was
R6610	T22870	T22855	conj	was,was
R6611	T22871	T22870	prep	below,was
R6612	T22872	T22873	det	the,level
R6613	T22873	T22871	pobj	level,below
R6614	T22874	T22873	prep	of,level
R6615	T22875	T22874	pobj	detection,of
R6616	T22876	T22870	prep	in,was
R6617	T22877	T22878	punct	-,-
R6618	T22878	T22880	punct	-,liver
R6619	T22879	T22878	punct	/,-
R6620	T22880	T22876	pobj	liver,in
R6621	T22881	T22855	punct	.,was
R6622	T22883	T22884	meta	B,concentrations
R6623	T22885	T22884	punct	", ",concentrations
R6624	T22886	T22884	amod	Total,concentrations
R6625	T22887	T22884	compound	BCAA,concentrations
R6626	T22888	T22884	prep	in,concentrations
R6627	T22889	T22888	pobj	blood,in
R6628	T22890	T22889	prep	of,blood
R6629	T22891	T22890	pobj	mice,of
R6630	T22892	T22884	punct	.,concentrations
R6631	T22894	T22895	amod	Total,BCAA
R6632	T22895	T22896	nsubj	BCAA,represent
R6633	T22897	T22898	det	the,sum
R6634	T22898	T22896	dobj	sum,represent
R6635	T22899	T22898	prep	of,sum
R6636	T22900	T22899	pobj	leucine,of
R6637	T22901	T22900	punct	", ",leucine
R6638	T22902	T22900	conj	isoleucine,leucine
R6639	T22903	T22902	punct	", ",isoleucine
R6640	T22904	T22902	cc	and,isoleucine
R6641	T22905	T22902	conj	valine,isoleucine
R6642	T22906	T22896	punct	.,represent
R6643	T22908	T22909	amod	Total,concentrations
R6644	T22909	T22911	nsubj	concentrations,were
R6645	T22910	T22909	compound	BCAA,concentrations
R6646	T22912	T22909	prep	in,concentrations
R6647	T22913	T22912	pobj	blood,in
R6648	T22914	T22913	prep	from,blood
R6649	T22915	T22916	punct	-,-
R6650	T22916	T22918	punct	-,mice
R6651	T22917	T22916	punct	/,-
R6652	T22918	T22914	pobj	mice,from
R6653	T22919	T22920	advmod	significantly,elevated
R6654	T22920	T22911	acomp	elevated,were
R6655	T22921	T22911	prep	compared,were
R6656	T22922	T22921	prep	to,compared
R6657	T22923	T22924	punct	+,+
R6658	T22924	T22922	punct	+,to
R6659	T22925	T22924	punct	/,+
R6660	T22926	T22924	cc	and,+
R6661	T22927	T22928	punct	+,-
R6662	T22928	T22924	conj	-,+
R6663	T22929	T22928	punct	/,-
R6664	T22930	T22911	punct	.,were
R6665	T22932	T22933	meta	C,Ratio
R6666	T22934	T22933	punct	", ",Ratio
R6667	T22935	T22933	prep	of,Ratio
R6668	T22936	T22937	amod	total,BCAA
R6669	T22937	T22935	pobj	BCAA,of
R6670	T22938	T22933	prep	to,Ratio
R6671	T22939	T22938	pobj	alanine,to
R6672	T22940	T22933	prep	in,Ratio
R6673	T22941	T22940	pobj	blood,in
R6674	T22942	T22941	prep	of,blood
R6675	T22943	T22942	pobj	mice,of
R6676	T22944	T22933	punct	.,Ratio
R6677	T22946	T22947	det	This,ratio
R6678	T22947	T22948	nsubj	ratio,was
R6679	T22949	T22950	advmod	significantly,elevated
R6680	T22950	T22948	acomp	elevated,was
R6681	T22951	T22948	prep	in,was
R6682	T22952	T22953	punct	-,-
R6683	T22953	T22955	punct	-,mice
R6684	T22954	T22953	punct	/,-
R6685	T22955	T22951	pobj	mice,in
R6686	T22956	T22948	prep	compared,was
R6687	T22957	T22956	prep	to,compared
R6688	T22958	T22959	punct	+,+
R6689	T22959	T22961	punct	+,mice
R6690	T22960	T22959	punct	/,+
R6691	T22961	T22957	pobj	mice,to
R6692	T22962	T22959	cc	and,+
R6693	T22963	T22964	punct	+,-
R6694	T22964	T22959	conj	-,+
R6695	T22965	T22964	punct	/,-
R6696	T22966	T22948	punct	.,was
R6697	T22968	T22969	det	The,numbers
R6698	T22969	T22970	nsubj	numbers,indicates
R6699	T22971	T22969	prep	on,numbers
R6700	T22972	T22973	det	the,bars
R6701	T22973	T22971	pobj	bars,on
R6702	T22974	T22975	det	the,number
R6703	T22975	T22970	dobj	number,indicates
R6704	T22976	T22975	prep	of,number
R6705	T22977	T22976	pobj	mice,of
R6706	T22978	T22977	acl	analyzed,mice
R6707	T22979	T22970	punct	.,indicates
R6708	T22981	T22982	punct	,*
R6709	T22983	T22981	punct	", ",*
R6710	T22984	T22985	advmod	Significantly,different
R6711	T22985	T22981	amod	different,*
R6712	T22986	T22985	prep	from,different
R6713	T22987	T22988	punct	+,+
R6714	T22988	T22986	punct	+,from
R6715	T22989	T22988	punct	/,+
R6716	T22990	T22991	punct	(,0.001
R6717	T22991	T22985	parataxis	0.001,different
R6718	T22992	T22991	nsubj	P,0.001
R6719	T22993	T22991	punct	<,0.001
R6720	T22994	T22991	punct	),0.001
R6721	T22995	T22982	punct	;,**
R6722	T22996	T22982	punct	", ",**
R6723	T22997	T22998	advmod	significantly,different
R6724	T22998	T22982	amod	different,**
R6725	T22999	T22998	prep	from,different
R6726	T23000	T23001	punct	+,+
R6727	T23001	T22999	punct	+,from
R6728	T23002	T23001	punct	/,+
R6729	T23003	T23001	cc	and,+
R6730	T23004	T23005	punct	+,-
R6731	T23005	T23001	conj	-,+
R6732	T23006	T23005	punct	/,-
R6733	T23007	T23008	punct	(,0.001
R6734	T23008	T22998	parataxis	0.001,different
R6735	T23009	T23008	nsubj	P,0.001
R6736	T23010	T23008	punct	<,0.001
R6737	T23011	T23008	punct	),0.001
R6738	T23012	T22982	punct	.,**
R6739	T23655	T23656	amod	Transgenic,production
R6740	T23657	T23656	compound	mouse,production
R6741	T23658	T23656	cc	and,production
R6742	T23659	T23656	conj	characterization,production
R6743	T23660	T23656	punct	.,production
R6744	T23662	T23663	meta	A,strategy
R6745	T23664	T23663	punct	", ",strategy
R6746	T23665	T23663	amod	Transgenic,strategy
R6747	T23666	T23663	acl	used,strategy
R6748	T23667	T23668	aux	to,produce
R6749	T23668	T23666	advcl	produce,used
R6750	T23669	T23668	dobj	mice,produce
R6751	T23670	T23671	dep	that,express
R6752	T23671	T23669	relcl	express,mice
R6753	T23672	T23673	amod	human,E2
R6754	T23673	T23671	dobj	E2,express
R6755	T23674	T23663	punct	.,strategy
R6756	T23676	T23677	nmod	LAP,tTA
R6757	T23677	T23679	nmod	tTA,mice
R6758	T23678	T23677	punct	-,tTA
R6759	T23679	T23681	nsubjpass	mice,described
R6760	T23680	T23679	amod	transgenic,mice
R6761	T23682	T23681	aux	have,described
R6762	T23683	T23681	auxpass	been,described
R6763	T23684	T23681	advmod	previously,described
R6764	T23685	T23686	punct	[,34
R6765	T23686	T23681	parataxis	34,described
R6766	T23687	T23686	punct	],34
R6767	T23688	T23681	punct	.,described
R6768	T23690	T23691	det	These,mice
R6769	T23691	T23692	nsubj	mice,express
R6770	T23693	T23694	det	the,transactivator
R6771	T23694	T23692	dobj	transactivator,express
R6772	T23695	T23696	npadvmod	tetracycline,controlled
R6773	T23696	T23694	amod	controlled,transactivator
R6774	T23697	T23696	punct	-,controlled
R6775	T23698	T23694	punct	(,transactivator
R6776	T23699	T23694	appos	tTA,transactivator
R6777	T23700	T23694	punct	),transactivator
R6778	T23701	T23694	prep	from,transactivator
R6779	T23702	T23703	det	the,promoter
R6780	T23703	T23701	pobj	promoter,from
R6781	T23704	T23705	npadvmod	liver,specific
R6782	T23705	T23703	amod	specific,promoter
R6783	T23706	T23703	compound	LAP,promoter
R6784	T23707	T23692	punct	.,express
R6785	T23709	T23710	det	The,transgene
R6786	T23710	T23714	nsubj	transgene,contains
R6787	T23711	T23712	compound	TRE,E2
R6788	T23712	T23710	compound	E2,transgene
R6789	T23713	T23712	punct	-,E2
R6790	T23715	T23716	det	the,element
R6791	T23716	T23714	dobj	element,contains
R6792	T23717	T23716	compound	tetracycline,element
R6793	T23718	T23716	compound	response,element
R6794	T23719	T23716	punct	(,element
R6795	T23720	T23716	appos	TRE,element
R6796	T23721	T23716	punct	),element
R6797	T23722	T23716	prep	as,element
R6798	T23723	T23722	pobj	part,as
R6799	T23724	T23723	prep	of,part
R6800	T23725	T23726	det	the,promoter
R6801	T23726	T23724	pobj	promoter,of
R6802	T23727	T23716	punct	", ",element
R6803	T23728	T23729	det	a,intron
R6804	T23729	T23716	conj	intron,element
R6805	T23730	T23729	amod	synthetic,intron
R6806	T23731	T23732	punct	(,line
R6807	T23732	T23729	parataxis	line,intron
R6808	T23733	T23732	amod	thin,line
R6809	T23734	T23732	punct	),line
R6810	T23735	T23729	punct	", ",intron
R6811	T23736	T23737	det	the,cDNA
R6812	T23737	T23729	conj	cDNA,intron
R6813	T23738	T23737	amod	human,cDNA
R6814	T23739	T23737	compound	E2,cDNA
R6815	T23740	T23737	punct	", ",cDNA
R6816	T23741	T23742	det	an,spacer
R6817	T23742	T23737	conj	spacer,cDNA
R6818	T23743	T23742	compound	alanine,spacer
R6819	T23744	T23742	punct	", ",spacer
R6820	T23745	T23746	det	a,tag
R6821	T23746	T23742	conj	tag,spacer
R6822	T23747	T23748	compound	c,myc
R6823	T23748	T23746	compound	myc,tag
R6824	T23749	T23748	punct	-,myc
R6825	T23750	T23746	compound	epitope,tag
R6826	T23751	T23746	punct	", ",tag
R6827	T23752	T23746	cc	and,tag
R6828	T23753	T23754	npadvmod	SV40,derived
R6829	T23754	T23755	amod	derived,sequence
R6830	T23755	T23746	conj	sequence,tag
R6831	T23756	T23755	compound	polyadenylation,sequence
R6832	T23757	T23714	punct	.,contains
R6833	T23759	T23760	det	This,construct
R6834	T23760	T23761	nsubjpass	construct,used
R6835	T23762	T23761	auxpass	was,used
R6836	T23763	T23764	aux	to,create
R6837	T23764	T23761	advcl	create,used
R6838	T23765	T23766	amod	several,lines
R6839	T23766	T23764	dobj	lines,create
R6840	T23767	T23766	prep	of,lines
R6841	T23768	T23769	amod	transgenic,mice
R6842	T23769	T23767	pobj	mice,of
R6843	T23770	T23761	punct	.,used
R6844	T23772	T23773	meta	B,analysis
R6845	T23774	T23773	punct	", ",analysis
R6846	T23775	T23776	compound	Western,blot
R6847	T23776	T23773	compound	blot,analysis
R6848	T23777	T23773	prep	of,analysis
R6849	T23778	T23779	compound	E2,protein
R6850	T23779	T23777	pobj	protein,of
R6851	T23780	T23779	prep	in,protein
R6852	T23781	T23780	pobj	liver,in
R6853	T23782	T23781	prep	of,liver
R6854	T23783	T23784	nmod	control,mice
R6855	T23784	T23782	pobj	mice,of
R6856	T23785	T23783	cc	and,control
R6857	T23786	T23783	conj	intermediate,control
R6858	T23787	T23784	compound	MSUD,mice
R6859	T23788	T23773	punct	.,analysis
R6860	T23791	T23792	mark	that,was
R6861	T23792	T23790	ccomp	was,Note
R6862	T23793	T23794	det	the,amount
R6863	T23794	T23792	nsubj	amount,was
R6864	T23795	T23794	prep	of,amount
R6865	T23796	T23797	amod	human,protein
R6866	T23797	T23795	pobj	protein,of
R6867	T23798	T23797	compound	E2,protein
R6868	T23799	T23800	punct	(,MW
R6869	T23800	T23797	parataxis	MW,protein
R6870	T23801	T23800	amod	predicted,MW
R6871	T23802	T23803	punct	~,54
R6872	T23803	T23804	nummod	54,Kd
R6873	T23804	T23800	appos	Kd,MW
R6874	T23805	T23800	punct	),MW
R6875	T23806	T23794	prep	in,amount
R6876	T23807	T23806	pobj	mice,in
R6877	T23808	T23807	prep	from,mice
R6878	T23809	T23810	compound	Lines,A
R6879	T23810	T23808	pobj	A,from
R6880	T23811	T23810	cc	and,A
R6881	T23812	T23813	nummod	525,A
R6882	T23813	T23810	conj	A,A
R6883	T23814	T23792	acomp	variable,was
R6884	T23815	T23792	cc	but,was
R6885	T23816	T23817	prep	in,was
R6886	T23817	T23792	conj	was,was
R6887	T23818	T23816	pobj	many,in
R6888	T23819	T23818	prep	of,many
R6889	T23820	T23821	det	the,animals
R6890	T23821	T23819	pobj	animals,of
R6891	T23822	T23823	det	the,amount
R6892	T23823	T23817	nsubj	amount,was
R6893	T23824	T23817	acomp	similar,was
R6894	T23825	T23824	prep	to,similar
R6895	T23826	T23827	det	the,amount
R6896	T23827	T23825	pobj	amount,to
R6897	T23828	T23827	prep	of,amount
R6898	T23829	T23830	compound	mouse,E2
R6899	T23830	T23828	pobj	E2,of
R6900	T23831	T23832	punct	(,Kd
R6901	T23832	T23830	parataxis	Kd,E2
R6902	T23833	T23832	nsubj	MW,Kd
R6903	T23834	T23832	punct	=,Kd
R6904	T23835	T23836	punct	~,47
R6905	T23836	T23832	nummod	47,Kd
R6906	T23837	T23832	punct	),Kd
R6907	T23838	T23827	prep	in,amount
R6908	T23839	T23840	compound	control,animals
R6909	T23840	T23838	pobj	animals,in
R6910	T23841	T23790	punct	.,Note
R6911	T23843	T23844	compound	Re,probing
R6912	T23844	T23846	nsubj	probing,confirmed
R6913	T23845	T23844	punct	-,probing
R6914	T23847	T23844	prep	with,probing
R6915	T23848	T23849	det	a,antibody
R6916	T23849	T23847	pobj	antibody,with
R6917	T23850	T23851	compound	c,myc
R6918	T23851	T23849	compound	myc,antibody
R6919	T23852	T23851	punct	-,myc
R6920	T23853	T23849	compound	tag,antibody
R6921	T23854	T23855	det	the,presence
R6922	T23855	T23846	dobj	presence,confirmed
R6923	T23856	T23855	prep	of,presence
R6924	T23857	T23858	det	the,E2
R6925	T23858	T23856	pobj	E2,of
R6926	T23859	T23860	npadvmod	transgene,derived
R6927	T23860	T23858	amod	derived,E2
R6928	T23861	T23858	punct	", ",E2
R6929	T23862	T23863	compound	c,myc
R6930	T23863	T23865	npadvmod	myc,tagged
R6931	T23864	T23863	punct	-,myc
R6932	T23865	T23858	amod	tagged,E2
R6933	T23866	T23858	punct	", ",E2
R6934	T23867	T23858	amod	human,E2
R6935	T23868	T23846	prep	in,confirmed
R6936	T23869	T23870	amod	transgenic,mice
R6937	T23870	T23868	pobj	mice,in
R6938	T23871	T23868	cc	but,in
R6939	T23872	T23871	neg	not,but
R6940	T23873	T23868	conj	in,in
R6941	T23874	T23873	pobj	controls,in
R6942	T23875	T23846	punct	.,confirmed
R6943	T23877	T23878	compound	Western,blot
R6944	T23878	T23879	compound	blot,analysis
R6945	T23879	T23880	nsubj	analysis,revealed
R6946	T23881	T23879	prep	of,analysis
R6947	T23882	T23881	pobj	brain,of
R6948	T23883	T23884	punct	(,C
R6949	T23884	T23882	parataxis	C,brain
R6950	T23885	T23884	punct	),C
R6951	T23886	T23882	punct	", ",brain
R6952	T23887	T23882	conj	kidney,brain
R6953	T23888	T23889	punct	(,D
R6954	T23889	T23887	parataxis	D,kidney
R6955	T23890	T23889	punct	),D
R6956	T23891	T23887	punct	", ",kidney
R6957	T23892	T23887	cc	and,kidney
R6958	T23893	T23887	conj	muscle,kidney
R6959	T23894	T23895	punct	(,E
R6960	T23895	T23893	parataxis	E,muscle
R6961	T23896	T23895	punct	),E
R6962	T23897	T23898	amod	negligible,amounts
R6963	T23898	T23880	dobj	amounts,revealed
R6964	T23899	T23898	prep	of,amounts
R6965	T23900	T23901	npadvmod	transgene,derived
R6966	T23901	T23902	amod	derived,E2
R6967	T23902	T23899	pobj	E2,of
R6968	T23903	T23880	prep	in,revealed
R6969	T23904	T23905	det	those,tissues
R6970	T23905	T23903	pobj	tissues,in
R6971	T23906	T23880	punct	.,revealed
R6972	T23908	T23909	det	All,blots
R6973	T23909	T23910	nsubjpass	blots,re-probed
R6974	T23911	T23910	auxpass	were,re-probed
R6975	T23912	T23910	prep	with,re-probed
R6976	T23913	T23914	det	an,antibody
R6977	T23914	T23912	pobj	antibody,with
R6978	T23915	T23914	compound	actin,antibody
R6979	T23916	T23917	aux	to,allow
R6980	T23917	T23910	advcl	allow,re-probed
R6981	T23918	T23919	nsubjpass	amount,compared
R6982	T23919	T23917	ccomp	compared,allow
R6983	T23920	T23918	prep	of,amount
R6984	T23921	T23920	pobj	protein,of
R6985	T23922	T23921	acl	loaded,protein
R6986	T23923	T23922	prep	in,loaded
R6987	T23924	T23925	det	each,lane
R6988	T23925	T23923	pobj	lane,in
R6989	T23926	T23919	aux	to,compared
R6990	T23927	T23919	auxpass	be,compared
R6991	T23928	T23910	punct	.,re-probed
R6992	T24446	T24445	prep	of,Characterization
R6993	T24447	T24448	det	the,model
R6994	T24448	T24446	pobj	model,of
R6995	T24449	T24448	amod	intermediate,model
R6996	T24450	T24448	nmod	MSUD,model
R6997	T24451	T24448	amod	murine,model
R6998	T24452	T24445	punct	.,Characterization
R6999	T24454	T24455	meta	A,analysis
R7000	T24456	T24455	punct	", ",analysis
R7001	T24457	T24455	compound	Survival,analysis
R7002	T24458	T24455	prep	of,analysis
R7003	T24459	T24460	amod	transgenic,lines
R7004	T24460	T24458	pobj	lines,of
R7005	T24461	T24460	compound	rescue,lines
R7006	T24462	T24455	punct	.,analysis
R7007	T24464	T24465	dep	Presented,are
R7008	T24466	T24465	nsubj	percentages,are
R7009	T24467	T24466	prep	of,percentages
R7010	T24468	T24467	pobj	mice,of
R7011	T24469	T24468	amod	alive,mice
R7012	T24470	T24469	prep	at,alive
R7013	T24471	T24470	pobj	weaning,at
R7014	T24472	T24468	prep	from,mice
R7015	T24473	T24474	det	each,line
R7016	T24474	T24472	pobj	line,from
R7017	T24475	T24474	amod	transgenic,line
R7018	T24476	T24474	acl	tested,line
R7019	T24477	T24478	dep	that,had
R7020	T24478	T24476	ccomp	had,tested
R7021	T24479	T24480	det	the,genotype
R7022	T24480	T24478	dobj	genotype,had
R7023	T24481	T24480	compound	rescue,genotype
R7024	T24482	T24483	punct	(,homozygous
R7025	T24483	T24478	parataxis	homozygous,had
R7026	T24484	T24483	advmod	i.e.,homozygous
R7027	T24485	T24483	punct	", ",homozygous
R7028	T24486	T24483	prep	for,homozygous
R7029	T24487	T24486	pobj	knockout,for
R7030	T24488	T24487	prep	of,knockout
R7031	T24489	T24490	amod	endogenous,E2
R7032	T24490	T24488	pobj	E2,of
R7033	T24491	T24483	cc	and,homozygous
R7034	T24492	T24483	conj	positive,homozygous
R7035	T24493	T24492	prep	for,positive
R7036	T24494	T24495	preconj	both,transgenes
R7037	T24495	T24493	pobj	transgenes,for
R7038	T24496	T24495	det	the,transgenes
R7039	T24497	T24498	nmod	LAP,tTA
R7040	T24498	T24495	nmod	tTA,transgenes
R7041	T24499	T24498	punct	-,tTA
R7042	T24500	T24498	cc	and,tTA
R7043	T24501	T24502	compound	TRE,E2
R7044	T24502	T24498	conj	E2,tTA
R7045	T24503	T24502	punct	-,E2
R7046	T24504	T24483	punct	),homozygous
R7047	T24505	T24465	punct	.,are
R7048	T24507	T24508	advmod	Also,is
R7049	T24509	T24508	dep	plotted,is
R7050	T24510	T24511	det	the,frequency
R7051	T24511	T24508	nsubj	frequency,is
R7052	T24512	T24511	amod	theoretical,frequency
R7053	T24513	T24511	amod	maximum,frequency
R7054	T24514	T24515	prep	at,expected
R7055	T24515	T24511	relcl	expected,frequency
R7056	T24516	T24514	pobj	which,at
R7057	T24517	T24518	det	this,genotype
R7058	T24518	T24515	nsubjpass	genotype,expected
R7059	T24519	T24515	auxpass	is,expected
R7060	T24520	T24515	prep	in,expected
R7061	T24521	T24522	det	this,population
R7062	T24522	T24520	pobj	population,in
R7063	T24523	T24522	prep	of,population
R7064	T24524	T24523	pobj	animals,of
R7065	T24525	T24508	punct	.,is
R7066	T24527	T24528	det	The,numbers
R7067	T24528	T24529	nsubj	numbers,indicates
R7068	T24530	T24528	prep	on,numbers
R7069	T24531	T24532	det	the,bars
R7070	T24532	T24530	pobj	bars,on
R7071	T24533	T24534	det	the,numbers
R7072	T24534	T24529	dobj	numbers,indicates
R7073	T24535	T24534	prep	of,numbers
R7074	T24536	T24535	pobj	observations,of
R7075	T24537	T24534	prep	for,numbers
R7076	T24538	T24539	det	each,line
R7077	T24539	T24537	pobj	line,for
R7078	T24540	T24529	punct	.,indicates
R7079	T24542	T24543	meta	B,Ratio
R7080	T24544	T24543	punct	", ",Ratio
R7081	T24545	T24543	prep	of,Ratio
R7082	T24546	T24547	amod	total,BCAA
R7083	T24547	T24545	pobj	BCAA,of
R7084	T24548	T24543	prep	to,Ratio
R7085	T24549	T24548	pobj	alanine,to
R7086	T24550	T24543	prep	in,Ratio
R7087	T24551	T24550	pobj	blood,in
R7088	T24552	T24551	prep	of,blood
R7089	T24553	T24552	pobj	mice,of
R7090	T24554	T24543	prep	from,Ratio
R7091	T24555	T24554	pobj	controls,from
R7092	T24556	T24555	cc	and,controls
R7093	T24557	T24558	compound	Lines,A
R7094	T24558	T24555	conj	A,controls
R7095	T24559	T24558	cc	and,A
R7096	T24560	T24558	conj	525A,A
R7097	T24561	T24543	punct	.,Ratio
R7098	T24563	T24564	amod	Total,BCAA
R7099	T24564	T24565	nsubj	BCAA,represent
R7100	T24566	T24567	det	the,sum
R7101	T24567	T24565	dobj	sum,represent
R7102	T24568	T24567	prep	of,sum
R7103	T24569	T24568	pobj	leucine,of
R7104	T24570	T24569	punct	", ",leucine
R7105	T24571	T24569	conj	isoleucine,leucine
R7106	T24572	T24571	punct	", ",isoleucine
R7107	T24573	T24571	cc	and,isoleucine
R7108	T24574	T24571	conj	valine,isoleucine
R7109	T24575	T24565	punct	.,represent
R7110	T24577	T24578	compound	BCAA,alanine
R7111	T24578	T24580	compound	alanine,values
R7112	T24579	T24578	punct	/,alanine
R7113	T24580	T24581	nsubj	values,were
R7114	T24582	T24583	advmod	significantly,greater
R7115	T24583	T24581	acomp	greater,were
R7116	T24584	T24583	prep	for,greater
R7117	T24585	T24586	compound	Lines,A
R7118	T24586	T24584	pobj	A,for
R7119	T24587	T24586	cc	and,A
R7120	T24588	T24586	conj	525A,A
R7121	T24589	T24583	prep	compared,greater
R7122	T24590	T24589	prep	to,compared
R7123	T24591	T24590	pobj	controls,to
R7124	T24592	T24581	prep	at,were
R7125	T24593	T24594	det	all,ages
R7126	T24594	T24592	pobj	ages,at
R7127	T24595	T24594	acl	tested,ages
R7128	T24596	T24581	punct	.,were
R7129	T24598	T24599	det	The,numbers
R7130	T24599	T24600	nsubj	numbers,indicates
R7131	T24601	T24599	prep	on,numbers
R7132	T24602	T24603	det	the,bars
R7133	T24603	T24601	pobj	bars,on
R7134	T24604	T24605	det	the,numbers
R7135	T24605	T24600	dobj	numbers,indicates
R7136	T24606	T24605	prep	of,numbers
R7137	T24607	T24606	pobj	samples,of
R7138	T24608	T24607	acl	analyzed,samples
R7139	T24609	T24600	punct	.,indicates
R7140	T24611	T24612	punct	*,0.01
R7141	T24612	T24616	ccomp	0.01,0.005
R7142	T24613	T24612	punct	", ",0.01
R7143	T24614	T24612	nsubj	P,0.01
R7144	T24615	T24612	punct	≤,0.01
R7145	T24617	T24616	punct	;,0.005
R7146	T24618	T24616	punct	**,0.005
R7147	T24619	T24616	punct	", ",0.005
R7148	T24620	T24616	nsubj	P,0.005
R7149	T24621	T24616	punct	<,0.005
R7150	T24622	T24616	punct	.,0.005
R7151	T24624	T24625	meta	C,activity
R7152	T24626	T24625	punct	", ",activity
R7153	T24627	T24625	compound	BCKDH,activity
R7154	T24628	T24625	compound	enzyme,activity
R7155	T24629	T24625	prep	in,activity
R7156	T24630	T24629	pobj	liver,in
R7157	T24631	T24630	prep	of,liver
R7158	T24632	T24633	nmod	control,mice
R7159	T24633	T24631	pobj	mice,of
R7160	T24634	T24632	cc	and,control
R7161	T24635	T24636	compound	Lines,A
R7162	T24636	T24632	conj	A,control
R7163	T24637	T24636	cc	and,A
R7164	T24638	T24636	conj	525A,A
R7165	T24639	T24625	punct	.,activity
R7166	T24641	T24642	punct	*,0.001
R7167	T24642	T24646	ccomp	0.001,0.0001
R7168	T24643	T24642	punct	", ",0.001
R7169	T24644	T24642	nsubj	P,0.001
R7170	T24645	T24642	punct	≤,0.001
R7171	T24647	T24646	punct	;,0.0001
R7172	T24648	T24646	punct	**,0.0001
R7173	T24649	T24646	punct	", ",0.0001
R7174	T24650	T24646	nsubj	P,0.0001
R7175	T24651	T24646	punct	≤,0.0001
R7176	T24652	T24646	punct	.,0.0001
R7177	T24654	T24655	meta	D,curves
R7178	T24656	T24655	punct	", ",curves
R7179	T24657	T24655	compound	Survival,curves
R7180	T24658	T24655	prep	for,curves
R7181	T24659	T24658	pobj	mice,for
R7182	T24660	T24659	prep	from,mice
R7183	T24661	T24662	compound	Lines,A
R7184	T24662	T24660	pobj	A,from
R7185	T24663	T24662	cc	and,A
R7186	T24664	T24662	conj	525A,A
R7187	T24665	T24655	punct	.,curves
R7188	T24667	T24668	compound	Rescue,mice
R7189	T24668	T24669	nsubjpass	mice,monitored
R7190	T24670	T24668	amod	alive,mice
R7191	T24671	T24670	prep	at,alive
R7192	T24672	T24671	pobj	weaning,at
R7193	T24673	T24669	auxpass	were,monitored
R7194	T24674	T24675	mark	until,were
R7195	T24675	T24669	advcl	were,monitored
R7196	T24676	T24675	nsubj	they,were
R7197	T24677	T24675	acomp	moribund,were
R7198	T24678	T24677	cc	and,moribund
R7199	T24679	T24680	advmod	subsequently,sacrificed
R7200	T24680	T24677	conj	sacrificed,moribund
R7201	T24681	T24675	cc	or,were
R7202	T24682	T24683	auxpass	were,found
R7203	T24683	T24675	conj	found,were
R7204	T24684	T24683	advcl	dead,found
R7205	T24685	T24683	prep	in,found
R7206	T24686	T24687	poss	their,cages
R7207	T24687	T24685	pobj	cages,in
R7208	T24688	T24669	punct	.,monitored
R7209	T24775	T24774	prep	of,Summary
R7210	T24776	T24777	amod	additional,levels
R7211	T24777	T24775	pobj	levels,of
R7212	T24778	T24777	compound	blood,levels
R7213	T24779	T24780	compound	amino,acid
R7214	T24780	T24777	compound	acid,levels
R7215	T24781	T24777	prep	in,levels
R7216	T24782	T24783	det	the,model
R7217	T24783	T24781	pobj	model,in
R7218	T24784	T24783	amod	classic,model
R7219	T24785	T24783	compound	MSUD,model
R7220	T24786	T24783	cc	and,model
R7221	T24787	T24788	compound	control,littermates
R7222	T24788	T24783	conj	littermates,model
R7223	T24789	T24790	mark	as,determined
R7224	T24790	T24777	advcl	determined,levels
R7225	T24791	T24790	prep	by,determined
R7226	T24792	T24793	amod	tandem,spectrometry
R7227	T24793	T24791	pobj	spectrometry,by
R7228	T24794	T24793	compound	mass,spectrometry
R7229	T24795	T24774	punct	.,Summary
R7230	T24797	T24798	det	All,samples
R7231	T24798	T24799	nsubjpass	samples,collected
R7232	T24800	T24799	auxpass	were,collected
R7233	T24801	T24799	prep	on,collected
R7234	T24802	T24803	det	the,day
R7235	T24803	T24801	pobj	day,on
R7236	T24804	T24803	prep	of,day
R7237	T24805	T24804	pobj	birth,of
R7238	T24806	T24799	punct	.,collected
R7239	T24808	T24809	det	All,values
R7240	T24809	T24810	nsubj	values,are
R7241	T24811	T24810	attr	mean,are
R7242	T24812	T24811	cc	+,mean
R7243	T24813	T24812	punct	/,+
R7244	T24814	T24812	punct	-,+
R7245	T24815	T24811	conj	SEM,mean
R7246	T24816	T24810	punct	.,are
R7247	T24915	T24914	prep	of,Summary
R7248	T24916	T24917	amod	additional,levels
R7249	T24917	T24915	pobj	levels,of
R7250	T24918	T24917	compound	blood,levels
R7251	T24919	T24920	compound	amino,acid
R7252	T24920	T24917	compound	acid,levels
R7253	T24921	T24917	prep	in,levels
R7254	T24922	T24923	det	the,model
R7255	T24923	T24921	pobj	model,in
R7256	T24924	T24923	amod	intermediate,model
R7257	T24925	T24923	compound	MSUD,model
R7258	T24926	T24923	cc	and,model
R7259	T24927	T24928	compound	littermate,controls
R7260	T24928	T24923	conj	controls,model
R7261	T24929	T24930	mark	as,determined
R7262	T24930	T24917	advcl	determined,levels
R7263	T24931	T24930	prep	by,determined
R7264	T24932	T24933	amod	tandem,spectrometry
R7265	T24933	T24931	pobj	spectrometry,by
R7266	T24934	T24933	compound	mass,spectrometry
R7267	T24935	T24914	punct	.,Summary
R7268	T24937	T24938	nsubjpass	Samples,collected
R7269	T24939	T24938	auxpass	were,collected
R7270	T24940	T24938	prep	from,collected
R7271	T24941	T24940	pobj	mice,from
R7272	T24942	T24943	dep	that,were
R7273	T24943	T24941	relcl	were,mice
R7274	T24944	T24945	quantmod	4,6
R7275	T24945	T24947	nummod	6,weeks
R7276	T24946	T24945	punct	–,6
R7277	T24947	T24943	attr	weeks,were
R7278	T24948	T24947	prep	of,weeks
R7279	T24949	T24948	pobj	age,of
R7280	T24950	T24938	punct	.,collected
R7281	T24952	T24953	det	All,values
R7282	T24953	T24954	nsubj	values,are
R7283	T24955	T24954	attr	mean,are
R7284	T24956	T24955	cc	+,mean
R7285	T24957	T24956	punct	/,+
R7286	T24958	T24956	punct	-,+
R7287	T24959	T24955	conj	SEM,mean
R7288	T24960	T24954	punct	.,are
R3345	T12266	T12268	punct	+,mice
R3347	T12268	T12264	pobj	mice,in
R3348	T12269	T12270	advmod	readily,detectable
R3349	T12270	T12256	acomp	detectable,was
R3350	T12271	T12256	punct	.,was
R3351	T12273	T12274	prep	In,was
R3352	T12275	T12276	amod	marked,contrast
R3353	T12276	T12273	pobj	contrast,In
R3354	T12277	T12274	punct	", ",was
R3355	T12278	T12279	compound	BCKDH,activity
R3356	T12279	T12274	nsubj	activity,was
R3357	T12280	T12281	advmod	completely,absent
R3358	T12281	T12274	acomp	absent,was
R3359	T12282	T12274	prep	in,was
R3360	T12283	T12284	punct	-,-
R3361	T12284	T12286	punct	-,livers
R3362	T12285	T12284	punct	/,-
R3410	T12339	T12340	nsubjpass	Immunohistochemistry,used
R3411	T12341	T12339	prep	with,Immunohistochemistry
R3412	T12342	T12343	det	an,antibody
R3413	T12343	T12341	pobj	antibody,with
R3414	T12344	T12345	npadvmod	E2,specific
R3415	T12345	T12343	amod	specific,antibody
R3416	T12346	T12340	auxpass	was,used
R3417	T12347	T12348	aux	to,examine
R3418	T12348	T12340	advcl	examine,used
R3419	T12349	T12350	compound	E2,protein
R3721	T12671	T12667	pobj	ratio,as
R3722	T12672	T12659	punct	.,expressed
R3723	T12674	T12675	mark	As,shown
R3724	T12675	T12676	advcl	shown,was
R3725	T12677	T12675	prep	in,shown
R3726	T12678	T12679	compound	Figure,2C
R3727	T12679	T12677	pobj	2C,in
R3728	T12680	T12676	punct	", ",was
R3729	T12681	T12682	det	this,ratio
R3730	T12682	T12676	nsubj	ratio,was
R3731	T12683	T12682	prep	in,ratio
R3732	T12684	T12685	punct	-,-
R3733	T12685	T12687	punct	-,pups
R3734	T12686	T12685	punct	/,-
R3735	T12687	T12683	pobj	pups,in
R3736	T12688	T12689	advmod	more,6
R3737	T12689	T12691	quantmod	6,fold
R3738	T12690	T12689	quantmod	than,6
R3739	T12691	T12693	advmod	fold,higher
R3740	T12692	T12691	punct	-,fold
R3741	T12693	T12676	acomp	higher,was
R3742	T12694	T12693	prep	than,higher
R3743	T12695	T12696	punct	+,+
R3744	T12696	T12698	punct	+,littermates
R3745	T12697	T12696	punct	/,+
R3746	T12698	T12694	pobj	littermates,than
R3747	T12699	T12696	cc	or,+
R3774	T12729	T12730	prep	In,lack
R3775	T12731	T12729	pobj	summary,In
R3776	T12732	T12730	punct	", ",lack
R3777	T12733	T12734	compound	E2,mice
R3778	T12734	T12730	nsubj	mice,lack
R3779	T12735	T12734	compound	knockout,mice
R3780	T12736	T12737	nmod	BCKDH,activity
R3781	T12737	T12730	dobj	activity,lack
R3782	T12738	T12737	amod	enzymatic,activity
R3783	T12739	T12737	punct	", ",activity
R3784	T12740	T12741	compound	E2,immunoreactivity
R3785	T12741	T12737	appos	immunoreactivity,activity
R3786	T12742	T12730	punct	", ",lack
R3787	T12743	T12730	cc	and,lack
R3788	T12744	T12730	conj	have,lack
R3789	T12745	T12746	advmod	markedly,elevated
R3790	T12746	T12747	amod	elevated,levels
R3791	T12747	T12744	dobj	levels,have
R3792	T12748	T12747	prep	of,levels
R3793	T12749	T12748	pobj	BCAA,of
R3794	T12750	T12744	prep	in,have
R3795	T12751	T12752	det	the,blood
R3796	T12752	T12750	pobj	blood,in
R3797	T12753	T12752	cc	and,blood
R3798	T12754	T12752	conj	urine,blood
R3799	T12755	T12730	punct	.,lack
R3800	T12757	T12758	det	These,derangements
R3801	T12758	T12760	nsubj	derangements,result
R3802	T12759	T12758	amod	metabolic,derangements
R3928	T15827	T15828	det	The,transgene
R3929	T15828	T15832	nsubj	transgene,directs
R3930	T15829	T15830	compound	LAP,tTA
R3931	T15830	T15828	compound	tTA,transgene
R3932	T15831	T15830	punct	-,tTA
R3933	T15833	T15834	punct	[,34
R3934	T15834	T15828	parataxis	34,transgene
R3935	T15835	T15834	punct	],34
R3936	T15836	T15837	amod	high,levels
R3937	T15837	T15832	dobj	levels,directs
R3938	T15838	T15837	prep	of,levels
R3939	T15839	T15840	npadvmod	liver,specific
R3940	T15840	T15841	amod	specific,expression
R3941	T15841	T15838	pobj	expression,of
R3942	T15842	T15841	prep	of,expression
R3943	T15843	T15844	det	the,transactivator
R3993	T15897	T15898	nmod	LAP,tTA
R3994	T15898	T15900	nmod	tTA,mice
R3995	T15899	T15898	punct	-,tTA
R3996	T15903	T15902	auxpass	w,e
R3997	T15904	T15902	advcl	re,e
R3998	T15919	T15920	amod	c,e
R3999	T15923	T15922	cc	and,d
R4000	T15924	T15922	conj	c,d
R4001	T15930	T15929	acl	a,r
R4002	T15932	T15928	aux	t,r
R4003	T15933	T15928	auxpass	e,r
R4004	T15937	T15936	agent	by,zed
R4005	T15938	T15939	det	the,laboratory
R4006	T15939	T15937	pobj	laboratory,by
R4007	T15940	T15939	compound	Bujard,laboratory
R4291	T16290	T16289	pcomp	rescuing,at
R4292	T16291	T16292	det	the,phenotype
R4294	T16292	T16290	dobj	phenotype,rescuing
R4295	T16293	T16292	amod	neonatal,phenotype
R4296	T16294	T16292	amod	lethal,phenotype
R4298	T16295	T16292	prep	of,phenotype
R4299	T16296	T16297	det	the,knockout
R4300	T16297	T16295	pobj	knockout,of
R4301	T16298	T16297	compound	E2,knockout
R4303	T16299	T16280	punct	.,appears
R4304	T16301	T16302	prep	In,failed
R4306	T16303	T16301	pobj	contrast,In
R4307	T16304	T16302	punct	", ",failed
R4308	T16305	T16306	compound	line,520B
R4310	T16306	T16302	nsubj	520B,failed
R4312	T16307	T16302	advmod	completely,failed
R4313	T16308	T16309	aux	to,rescue
R4314	T16309	T16302	xcomp	rescue,failed
R4315	T16310	T16311	det	the,phenotype
R4317	T16311	T16309	dobj	phenotype,rescue
R4319	T16312	T16311	amod	neonatal,phenotype
R4320	T16313	T16311	amod	lethal,phenotype
R4321	T16314	T16302	punct	.,failed
R4323	T16316	T16317	det	All,lines
R4324	T16317	T16319	nsubj	lines,produced
R4325	T16318	T16317	amod	other,lines
R4327	T16320	T16317	acl	tested,lines
R4329	T16321	T16322	amod	surviving,animals
R4330	T16322	T16319	dobj	animals,produced
R4331	T16323	T16322	compound	rescue,animals
R4333	T16324	T16319	prep	at,produced
R4334	T16325	T16326	det	a,frequency
R4335	T16326	T16324	pobj	frequency,at
R4337	T16327	T16326	prep	between,frequency
R4338	T16328	T16329	nummod	1,%
R4339	T16329	T16327	pobj	%,between
R4341	T16330	T16328	cc	and,1
R4342	T16331	T16328	conj	5,1
R4343	T16332	T16329	prep	of,%
R4353	T16341	T16340	nsubj	n,i
R4360	T16352	T16353	advmod	s,e
R4361	T16353	T16351	acomp	e,were
R4372	T16363	T16362	cc	d,or
R4369	T16380	T16381	compound	Fig.,4B
R4370	T16381	T16382	nsubj	4B,shows
R4371	T16383	T16384	det	the,results
R4373	T16384	T16382	dobj	results,shows
R4374	T16385	T16384	prep	of,results
R4375	T16367	T16366	pobj	ai,t
R4376	T16386	T16387	compound	blood,analysis
R4377	T16371	T16370	conj	c,d
R4378	T16387	T16385	pobj	analysis,of
R4379	T16372	T16373	poss	h,a
R4380	T16388	T16389	compound	amino,acid
R4381	T16389	T16387	compound	acid,analysis
R4382	T16376	T16377	amod	a,cterization
R4383	T16390	T16384	acl	presented,results
R4384	T16391	T16390	prep	as,presented
R4385	T16392	T16393	det	a,ratio
R4386	T16393	T16391	pobj	ratio,as
R4387	T16394	T16393	prep	of,ratio
R4389	T16395	T16396	amod	total,BCAA
R4392	T16396	T16394	pobj	BCAA,of
R4396	T16397	T16393	prep	to,ratio
R4400	T16398	T16397	pobj	alanine,to
R4533	T16440	T16439	pobj	controls,between
R4537	T16441	T16440	cc	and,controls
R4540	T16442	T16440	conj	knockouts,controls
R4544	T16443	T16444	punct	(,see
R4548	T16444	T16428	parataxis	see,are
R4551	T16445	T16446	compound	Figure,2C
R4552	T16446	T16444	dobj	2C,see
R4553	T16447	T16444	punct	),see
R4556	T16448	T16426	punct	.,Note
R4563	T16450	T16451	compound	Blood,levels
R4565	T16451	T16452	nsubjpass	levels,reduced
R4568	T16453	T16451	prep	of,levels
R4574	T16454	T16453	pobj	alanine,of
R4577	T16455	T16454	punct	", ",alanine
R4580	T16456	T16454	conj	glutamate,alanine
R4583	T16457	T16456	punct	", ",glutamate
R4586	T16458	T16456	cc	and,glutamate
R4590	T16459	T16456	conj	glutamine,glutamate
R4593	T16460	T16452	auxpass	were,reduced
R4597	T16461	T16452	prep	in,reduced
R4601	T16462	T16463	compound	Line,A
R4605	T16463	T16464	compound	A,mice
R4609	T16464	T16461	pobj	mice,in
R4616	T16465	T16452	punct	", ",reduced
R4620	T16466	T16467	mark	as,was
R4623	T16467	T16452	advcl	was,reduced
R4626	T16468	T16467	nsubj	glutamate,was
R4629	T16469	T16468	prep	in,glutamate
R4633	T16470	T16471	compound	Line,525A
R4636	T16471	T16472	compound	525A,mice
R4640	T16472	T16469	pobj	mice,in
R4644	T16473	T16468	punct	", ",glutamate
R4648	T16474	T16468	prep	compared,glutamate
R4652	T16475	T16474	prep	to,compared
R4656	T16476	T16475	pobj	controls,to
R4658	T16477	T16478	punct	(,Table
R4661	T16478	T16467	parataxis	Table,was
R4667	T16479	T16478	nummod	2,Table
R4671	T16480	T16478	punct	),Table
R4672	T16481	T16452	punct	.,reduced
R4388	T16484	T16485	mark	Because,demonstrated
R4390	T16485	T16503	advcl	demonstrated,examined
R4391	T16486	T16487	det	the,mice
R4393	T16487	T16485	nsubjpass	mice,demonstrated
R4394	T16488	T16489	compound	LAP,tTA
R4395	T16489	T16487	compound	tTA,mice
R4397	T16490	T16489	punct	-,tTA
R4398	T16491	T16492	dep	that,used
R4399	T16492	T16487	relcl	used,mice
R4401	T16493	T16492	auxpass	were,used
R4402	T16494	T16495	aux	to,drive
R4403	T16495	T16492	advcl	drive,used
R4404	T16496	T16495	dobj	expression,drive
R4405	T16497	T16496	prep	of,expression
R4407	T16498	T16499	amod	transgenic,E2
R4408	T16499	T16497	pobj	E2,of
R4409	T16500	T16499	amod	human,E2
R4411	T16501	T16485	aux	have,demonstrated
R4415	T16502	T16485	auxpass	been,demonstrated
R4417	T16504	T16505	aux	to,produce
R4418	T16505	T16485	xcomp	produce,demonstrated
R4419	T16506	T16507	npadvmod	liver,specific
R4421	T16507	T16508	amod	specific,expression
R4422	T16508	T16505	dobj	expression,produce
R4423	T16509	T16510	punct	[,34
R4425	T16510	T16485	parataxis	34,demonstrated
R4426	T16511	T16510	punct	],34
R4427	T16512	T16503	punct	", ",examined
R4429	T16513	T16514	compound	BCKDH,activity
R4430	T16514	T16503	nsubjpass	activity,examined
R4431	T16515	T16514	compound	enzyme,activity
R4433	T16516	T16514	cc	and,activity
R4434	T16517	T16514	conj	production,activity
R4435	T16518	T16517	prep	of,production
R4437	T16519	T16520	amod	human,protein
R4438	T16520	T16518	pobj	protein,of

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T24441	11932-11936	NCBITaxon:10088	denotes	mice
T24444	12046-12056	GO_EXT:killing	denotes	sacrificed
T24443	11967-11971	NCBITaxon:10088	denotes	mice
T24442	11960-11966	SO_EXT:sequence_rescue_process	denotes	Rescue
T24913	12842-12846	NCBITaxon:10088	denotes	mice
T24912	12698-12708	CHEBI_SO_EXT:amino_acid	denotes	amino acid
T24911	12692-12697	UBERON:0000178	denotes	blood
T24440	11873-11877	NCBITaxon:10088	denotes	mice
T24439	11835-11840	UBERON:0002107	denotes	liver
T24438	11816-11822	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T24437	11608-11615	CHEBI_SO_EXT:alanine	denotes	alanine
T24436	11603-11607	CHEBI:22918	denotes	BCAA
T24435	11595-11601	CHEBI_SO_EXT:valine	denotes	valine
T24434	11579-11589	CHEBI_SO_EXT:isoleucine	denotes	isoleucine
T24433	11570-11577	CHEBI_SO_EXT:leucine	denotes	leucine
T24432	11544-11548	CHEBI:22918	denotes	BCAA
T24431	11497-11501	NCBITaxon:10088	denotes	mice
T24430	11488-11493	UBERON:0000178	denotes	blood
T24429	11477-11484	CHEBI_SO_EXT:alanine	denotes	alanine
T24428	11469-11473	CHEBI:22918	denotes	BCAA
T24427	11365-11372	NCBITaxon:33208	denotes	animals
T24426	11322-11330	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T24425	11245-11255	SO_EXT:0000902	denotes	transgenes
T24424	11242-11244	PR_EXT:000006300	denotes	E2
T24423	11226-11229	PR_EXT:000005308	denotes	LAP
T24422	11197-11199	PR_EXT:000006300	denotes	E2
T24421	11143-11151	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T24420	11136-11142	SO_EXT:sequence_rescue_process	denotes	rescue
T24419	11100-11110	SO_EXT:transgenic_entity	denotes	transgenic
T24418	11068-11072	NCBITaxon:10088	denotes	mice
T24417	11025-11031	SO_EXT:sequence_rescue_process	denotes	rescue
T24416	11014-11024	SO_EXT:transgenic_entity	denotes	transgenic
T24415	10976-10982	NCBITaxon:10088	denotes	murine
T23645	8519-8523	NCBITaxon:10088	denotes	mice
T23644	8508-8518	SO_EXT:transgenic_entity	denotes	transgenic
T23643	8502-8504	PR_EXT:000006300	denotes	E2
T23642	8496-8501	NCBITaxon:9606	denotes	human
T23641	8488-8494	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	tagged
T23640	8482-8487	PR_EXT:000000084	denotes	c-myc
T23639	8463-8472	SO_EXT:0000902	denotes	transgene
T23638	8424-8432	GO:0042571	denotes	antibody
T23637	8420-8423	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	tag
T23636	8414-8419	PR_EXT:000000084	denotes	c-myc
T23635	8387-8394	NCBITaxon:33208	denotes	animals
T23634	8361-8363	PR_EXT:000006300	denotes	E2
T23633	8355-8360	NCBITaxon:10088	denotes	mouse
T23632	8307-8314	NCBITaxon:33208	denotes	animals
T23631	8247-8251	NCBITaxon:10088	denotes	mice
T23630	8214-8221	CHEBI_PR_EXT:protein	denotes	protein
T23629	8211-8213	PR_EXT:000006300	denotes	E2
T23628	8205-8210	NCBITaxon:9606	denotes	human
T23627	8175-8179	NCBITaxon:10088	denotes	mice
T23626	8136-8141	UBERON:0002107	denotes	liver
T23625	8125-8132	CHEBI_PR_EXT:protein	denotes	protein
T23624	8122-8124	PR_EXT:000006300	denotes	E2
T23623	8088-8092	NCBITaxon:10088	denotes	mice
T23622	8077-8087	SO_EXT:transgenic_entity	denotes	transgenic
T23621	8031-8040	SO_EXT:engineered_biological_sequence	denotes	construct
T23620	8000-8024	SO_EXT:0000610	denotes	polyadenylation sequence
T23619	8000-8015	GO:0043631	denotes	polyadenylation
T23618	7987-7991	NCBITaxon:10633	denotes	SV40
T23617	7978-7981	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	tag
T23616	7970-7977	CHEBI_SO_EXT:epitope	denotes	epitope
T23615	7964-7969	PR_EXT:000000084	denotes	c-myc
T23614	7946-7953	CHEBI_SO_EXT:alanine	denotes	alanine
T23613	7937-7941	SO_EXT:cDNA	denotes	cDNA
T23612	7934-7936	PR_EXT:000006300	denotes	E2
T23611	7928-7933	NCBITaxon:9606	denotes	human
T23610	7904-7910	SO_EXT:0000188	denotes	intron
T23609	7882-7890	SO_EXT:0000167	denotes	promoter
T23608	7844-7852	GO_EXT:reaction_or_response	denotes	response
T23607	7831-7843	CHEBI:27902	denotes	tetracycline
T23606	7808-7817	SO_EXT:0000902	denotes	transgene
T23605	7805-7807	PR_EXT:000006300	denotes	E2
T23604	7787-7795	SO_EXT:0000167	denotes	promoter
T23603	7783-7786	PR_EXT:000005308	denotes	LAP
T23602	7768-7773	UBERON:0002107	denotes	liver
T23601	7727-7737	GO:0065007	denotes	controlled
T23600	7714-7726	CHEBI:27902	denotes	tetracycline
T23599	7702-7709	GO:0010467	denotes	express
T23598	7697-7701	NCBITaxon:10088	denotes	mice
T23597	7649-7653	NCBITaxon:10088	denotes	mice
T23596	7638-7648	SO_EXT:transgenic_entity	denotes	transgenic
T23595	7630-7633	PR_EXT:000005308	denotes	LAP
T23594	7626-7628	PR_EXT:000006300	denotes	E2
T23593	7620-7625	NCBITaxon:9606	denotes	human
T23592	7612-7619	GO:0010467	denotes	express
T23591	7602-7606	NCBITaxon:10088	denotes	mice
T23590	7566-7576	SO_EXT:transgenic_entity	denotes	Transgenic
T23589	7524-7529	NCBITaxon:10088	denotes	mouse
T23588	7513-7523	SO_EXT:transgenic_entity	denotes	Transgenic
T23654	8745-8752	CHEBI_PR_EXT:protein	denotes	protein
T23653	8717-8725	GO:0042571	denotes	antibody
T23652	8711-8716	PR_EXT:000003676	denotes	actin
T23651	8669-8676	UBERON:0000479	denotes	tissues
T23650	8657-8659	PR_EXT:000006300	denotes	E2
T23649	8639-8648	SO_EXT:0000902	denotes	transgene
T23648	8597-8603	UBERON_EXT:muscle_structure_or_tissue	denotes	muscle
T23647	8581-8587	UBERON:0002113	denotes	kidney
T23646	8570-8575	UBERON:0000955	denotes	brain
T15725	15896-15900	NCBITaxon:10088	denotes	mice
T15724	15889-15895	SO_EXT:sequence_rescue_process	denotes	rescue
T15723	15718-15723	UBERON:0002107	denotes	liver
T15722	15695-15701	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T15721	15663-15672	GO:0010467	denotes	expressed
T15720	15585-15592	CHEBI_SO_EXT:alanine	denotes	alanine
T15718	15571-15575	NCBITaxon:10088	denotes	mice
T15717	15511-15515	GO:0016265	denotes	died
T15716	15453-15457	NCBITaxon:10088	denotes	mice
T15715	15325-15335	GO_EXT:killing	denotes	sacrificed
T15714	15283-15287	NCBITaxon:10088	denotes	mice
T15713	15244-15248	NCBITaxon:10088	denotes	mice
T15712	15237-15243	SO_EXT:sequence_rescue_process	denotes	rescue
T15711	15166-15173	NCBITaxon:33208	denotes	animals
T15710	15062-15072	SO_EXT:transgenic_entity	denotes	transgenic
T15709	15042-15046	NCBITaxon:10088	denotes	mice
T15708	15007-15014	UBERON:0000479	denotes	tissues
T15707	14981-14983	PR_EXT:000006300	denotes	E2
T15706	14967-14977	GO:0010467	denotes	expression
T15705	14913-14919	UBERON_EXT:muscle_structure_or_tissue	denotes	muscle
T15704	14902-14908	UBERON:0002113	denotes	kidney
T15703	14895-14900	UBERON:0000955	denotes	brain
T15702	14882-14891	SO_EXT:0000902	denotes	transgene
T15701	14879-14881	PR_EXT:000006300	denotes	E2
T15700	14861-14871	GO:0010467	denotes	expression
T15699	14797-14807	CHEBI_SO_EXT:amino_acid	denotes	amino acid
T15698	14787-14789	PR_EXT:000006300	denotes	E2
T15697	14781-14786	NCBITaxon:10088	denotes	mouse
T15696	14754-14756	PR_EXT:000006300	denotes	E2
T15673	14136-14138	PR_EXT:000006300	denotes	E2
T15672	14118-14127	SO_EXT:0000902	denotes	transgene
T15671	14089-14095	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T15670	14044-14051	CHEBI_PR_EXT:protein	denotes	protein
T15669	14041-14043	PR_EXT:000006300	denotes	E2
T15668	13984-13988	NCBITaxon:10088	denotes	mice
T15667	13965-13975	SO_EXT:transgenic_entity	denotes	transgenic
T15666	13953-13958	UBERON:0002107	denotes	liver
T15665	13929-13931	PR_EXT:000006300	denotes	E2
T15664	13923-13928	NCBITaxon:10088	denotes	mouse
T15663	13879-13881	PR_EXT:000006300	denotes	E2
T15662	13873-13878	NCBITaxon:9606	denotes	human
T15661	13854-13858	NCBITaxon:10088	denotes	mice
T15660	13843-13853	SO_EXT:transgenic_entity	denotes	transgenic
T15659	13820-13824	NCBITaxon:10088	denotes	mice
T15658	13788-13792	NCBITaxon:10088	denotes	mice
T15657	13744-13746	PR_EXT:000006300	denotes	E2
T15656	13738-13743	NCBITaxon:9606	denotes	human
T15655	13686-13691	UBERON:0002107	denotes	liver
T15654	13648-13657	CHEBI_GO_SO_EXT:enzyme	denotes	enzymatic
T15653	13584-13589	UBERON:0002107	denotes	liver
T15652	13562-13571	CHEBI_GO_SO_EXT:enzyme	denotes	enzymatic
T15651	13516-13521	UBERON:0002107	denotes	liver
T15650	13505-13512	CHEBI_PR_EXT:protein	denotes	protein
T15649	13502-13504	PR_EXT:000006300	denotes	E2
T15648	13496-13501	NCBITaxon:9606	denotes	human
T15647	13505-13512	GO:0006412	denotes	protein
T15646	13482-13495	_FRAGMENT	denotes	production of
T15645	13462-13468	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T15644	13439-13449	GO:0010467	denotes	expression
T15643	13424-13429	UBERON:0002107	denotes	liver
T15642	13387-13389	PR_EXT:000006300	denotes	E2
T15641	13381-13386	NCBITaxon:9606	denotes	human
T15640	13370-13380	SO_EXT:transgenic_entity	denotes	transgenic
T15639	13356-13366	GO:0010467	denotes	expression
T15638	13327-13331	NCBITaxon:10088	denotes	mice
T15637	13319-13322	PR_EXT:000005308	denotes	LAP
T15636	12623-12627	NCBITaxon:10088	denotes	mice
T15635	12600-12609	CHEBI_EXT:glutamate	denotes	glutamate
T15634	12587-12591	NCBITaxon:10088	denotes	mice
T15633	12554-12563	CHEBI_SO_EXT:glutamine	denotes	glutamine
T15632	12539-12548	CHEBI_EXT:glutamate	denotes	glutamate
T15631	12530-12537	CHEBI_SO_EXT:alanine	denotes	alanine
T15630	12514-12519	UBERON:0000178	denotes	Blood
T15629	12444-12448	NCBITaxon:10088	denotes	mice
T15628	12433-12443	SO_EXT:transgenic_entity	denotes	transgenic
T15627	12410-12417	CHEBI_SO_EXT:alanine	denotes	alanine
T15626	12405-12409	CHEBI:22918	denotes	BCAA
T15625	12284-12288	NCBITaxon:10088	denotes	mice
T15599	13777-13787	SO_EXT:transgenic_entity	denotes	transgenic
T15598	10574-10578	NCBITaxon_UBERON_EXT:pup	denotes	pups
T15597	10503-10507	NCBITaxon:10088	denotes	mice
T15596	10496-10502	SO_EXT:sequence_rescue_process	denotes	rescue
T15595	10379-10389	SO_EXT:transgenic_entity	denotes	transgenic
T15594	10360-10368	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T15593	10353-10359	SO_EXT:sequence_rescue_process	denotes	rescue
T15592	10322-10326	NCBITaxon:10088	denotes	mice
T15591	10270-10280	SO_EXT:0000902	denotes	transgenes
T15590	10236-10238	PR_EXT:000006300	denotes	E2
T15589	10209-10217	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T15588	10202-10208	SO_EXT:sequence_rescue_process	denotes	rescue
T15587	10185-10192	NCBITaxon:33208	denotes	animals
T15586	10117-10125	GO_EXT:breeding	denotes	breeding
T15585	10068-10077	GO_EXT:fatality_or_lethality	denotes	lethality
T15584	10059-10067	PATO_UBERON_EXT:neonate_or_newborn	denotes	neonatal
T15583	10034-10040	SO_EXT:sequence_rescue_process	denotes	rescue
T15582	10020-10030	SO_EXT:0000902	denotes	transgenes
T15581	9962-9964	PR_EXT:000006300	denotes	E2
T15580	9943-9952	SO_EXT:0000902	denotes	transgene
T15579	9940-9942	PR_EXT:000006300	denotes	E2
T15578	9921-9930	SO_EXT:0000902	denotes	transgene
T15577	9913-9916	PR_EXT:000005308	denotes	LAP
T15576	9882-9886	NCBITaxon:10088	denotes	mice
T15575	9844-9852	GO_EXT:breeding	denotes	breeding
T15574	9819-9821	PR_EXT:000006300	denotes	E2
T15573	9779-9788	SO_EXT:0000902	denotes	transgene
T15572	9771-9774	PR_EXT:000005308	denotes	LAP
T15571	9739-9743	NCBITaxon:10088	denotes	mice
T15570	9669-9673	NCBITaxon:10088	denotes	mice
T15569	9666-9668	PR_EXT:000006300	denotes	E2
T15568	9651-9661	SO_EXT:transgenic_entity	denotes	Transgenic
T15567	9629-9632	CHEBI_SO_EXT:DNA	denotes	DNA
T15566	9624-9628	UBERON:0002415	denotes	tail
T15540	8797-8801	NCBITaxon:10088	denotes	mice
T15539	8789-8792	PR_EXT:000005308	denotes	LAP
T15538	7469-7477	SO_EXT:0000167	denotes	promoter
T15537	7434-7438	SO_EXT:cDNA	denotes	cDNA
T15536	7431-7433	PR_EXT:000006300	denotes	E2
T15535	7425-7430	NCBITaxon:9606	denotes	human
T15534	7415-7422	GO:0010467	denotes	express
T15533	7389-7398	SO_EXT:0000902	denotes	transgene
T15532	7386-7388	PR_EXT:000006300	denotes	E2
T15531	7354-7362	GO_EXT:reaction_or_response	denotes	response
T15530	7315-7324	SO_EXT:0000167	denotes	promoters
T15529	7301-7311	GO:0010467	denotes	expression
T15528	7264-7284	GO_EXT:transcription_factor	denotes	transcription factor
T15527	7264-7277	GO_EXT:transcription	denotes	transcription
T15526	7229-7239	GO:0065007	denotes	controlled
T15525	7216-7228	CHEBI:27902	denotes	tetracycline
T15524	7198-7208	GO:0010467	denotes	expression
T15523	7183-7188	UBERON:0002107	denotes	liver
T15522	7160-7167	GO_EXT:biological_direction_or_guidance	denotes	directs
T15521	7145-7154	SO_EXT:0000902	denotes	transgene
T15520	7137-7140	PR_EXT:000005308	denotes	LAP
T15519	7112-7121	SO_EXT:0000902	denotes	transgene
T15518	7109-7111	PR_EXT:000006300	denotes	E2
T15517	7089-7098	SO_EXT:0000902	denotes	transgene
T15516	7081-7084	PR_EXT:000005308	denotes	LAP
T15515	7048-7058	SO_EXT:transgenic_entity	denotes	transgenic
T15514	7016-7018	PR_EXT:000006300	denotes	E2
T15513	7003-7008	UBERON:0002107	denotes	liver
T15695	14748-14753	NCBITaxon:9606	denotes	Human
T15694	14735-14746	PR_EXT:000006506	denotes	E3 subunits
T15693	14722-14727	NCBITaxon:10088	denotes	mouse
T15692	14707-14716	GO:0032991	denotes	complexed
T15691	14674-14676	PR_EXT:000006300	denotes	E2
T15690	14668-14673	NCBITaxon:9606	denotes	human
T15689	14620-14636	GO:0065003	denotes	subunit assembly
T15688	14581-14588	NCBITaxon:562	denotes	E. coli
T15687	14548-14564	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	carboxy terminus
T15686	14537-14540	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	tag
T15685	14516-14525	SO_EXT:sequence_insertion_entity_or_process	denotes	insertion
T15684	14438-14468	GO:0045254	denotes	pyruvate dehydrogenase complex
T15683	14438-14460	GO_EXT:0004738	denotes	pyruvate dehydrogenase
T15682	14438-14446	CHEBI:15361	denotes	pyruvate
T15681	14390-14406	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	carboxy terminus
T15680	14285-14294	CHEBI_GO_SO_EXT:enzyme	denotes	enzymatic
T15679	14266-14268	PR_EXT:000006300	denotes	E2
T15678	14260-14265	NCBITaxon:9606	denotes	human
T15677	14242-14251	SO_EXT:0000902	denotes	transgene
T15676	14218-14234	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	carboxy terminus
T15675	14207-14210	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	tag
T15674	14201-14206	PR_EXT:000000084	denotes	c-myc
T12205	6579-6583	NCBITaxon:10088	denotes	mice
T12204	6567-6569	PR_EXT:000006300	denotes	E2
T12203	6523-6529	NCBITaxon:9606	denotes	humans
T12202	6452-6461	GO_EXT:fatality_or_lethality	denotes	lethality
T12201	6443-6451	PATO_UBERON_EXT:neonate_or_newborn	denotes	neonatal
T12200	6399-6408	GO:0008152	denotes	metabolic
T12199	6386-6391	UBERON:0001088	denotes	urine
T12198	6376-6381	UBERON:0000178	denotes	blood
T12197	6364-6368	CHEBI:22918	denotes	BCAA
T12196	6306-6308	PR_EXT:000006300	denotes	E2
T12195	6286-6295	CHEBI_GO_SO_EXT:enzyme	denotes	enzymatic
T12194	6270-6274	NCBITaxon:10088	denotes	mice
T12193	6258-6260	PR_EXT:000006300	denotes	E2
T10230	23-25	PR_EXT:000006300	denotes	E2
T10231	26-30	SO_EXT:0000704	denotes	gene
T10232	40-44	NCBITaxon:10088	denotes	mice
T10233	55-57	PR_EXT:000006300	denotes	E2
T10234	67-71	NCBITaxon:10088	denotes	mice
T10235	81-85	SO_EXT:0000704	denotes	gene
T10236	99-104	NCBITaxon:10088	denotes	mouse
T10237	105-113	CL:0002322	denotes	ES cells
T10238	108-113	CL_GO_EXT:cell	denotes	cells
T10239	129-131	PR_EXT:000006300	denotes	E2
T10240	132-136	SO_EXT:0000704	denotes	gene
T10241	178-180	PR_EXT:000006300	denotes	E2
T10242	181-185	SO_EXT:0000704	denotes	gene
T10243	217-221	SO_EXT:0000704	denotes	gene
T10244	232-241	SO_EXT:engineered_biological_sequence	denotes	construct
T10245	258-265	SO_EXT:sequence_substitution_process	denotes	replace
T10246	274-275	CHEBI_SO_EXT:base	denotes	b
T10247	287-298	SO_EXT:genomic_DNA	denotes	genomic DNA
T10248	295-298	CHEBI_SO_EXT:DNA	denotes	DNA
T10249	321-333	SO_EXT:0000852	denotes	part of Exon
T10250	347-351	SO_EXT:0000147	denotes	Exon
T10251	381-387	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	marker
T10252	405-412	CL:0002322	denotes	ES cell
T10253	408-412	CL_GO_EXT:cell	denotes	cell
T10254	413-419	SO_EXT:sequence_cloned_entity	denotes	clones
T10255	502-543	SO_EXT:0000193	denotes	restriction fragment length polymorphisms
T10256	558-562	SO_EXT:0000704	denotes	gene
T10257	580-582	PR_EXT:000006300	denotes	E2
T10258	627-629	PR_EXT:000006300	denotes	E2
T10259	630-634	SO_EXT:0000147	denotes	Exon
T10260	646-651	CHEBI_SO_EXT:molecular_probe	denotes	probe
T10261	688-697	SO_EXT:engineered_biological_sequence	denotes	construct
T10262	699-709	GO:0097617	denotes	hybridizes
T10263	725-726	CHEBI_SO_EXT:base	denotes	b
T10264	732-752	SO_EXT:0000412	denotes	restriction fragment
T10265	762-771	SO_EXT:wild_type_entity_or_quality	denotes	wild type
T10266	772-778	SO_EXT:0001023	denotes	allele
T10267	798-805	CL:0002322	denotes	ES cell
T10268	801-805	CL_GO_EXT:cell	denotes	cell
T10269	834-842	CL:0002322	denotes	ES cells
T10270	837-842	CL_GO_EXT:cell	denotes	cells
T10271	849-854	CHEBI_SO_EXT:molecular_probe	denotes	probe
T10272	860-870	GO:0097617	denotes	hybridizes
T10273	881-882	CHEBI_SO_EXT:base	denotes	b
T10274	888-908	SO_EXT:0000412	denotes	restriction fragment
T10275	958-977	GO_EXT:0015666	denotes	restriction enzymes
T10276	970-977	CHEBI_GO_SO_EXT:enzyme	denotes	enzymes
T10277	982-988	CHEBI_SO_EXT:molecular_probe	denotes	probes
T10278	1026-1034	CL:0002322	denotes	ES cells
T10279	1029-1034	CL_GO_EXT:cell	denotes	cells
T10280	1059-1070	UBERON:0000358	denotes	blastocysts
T10281	1091-1095	NCBITaxon:10088	denotes	mice
T10282	1112-1116	GO_EXT:breeding	denotes	bred
T10283	1129-1133	NCBITaxon:10088	denotes	mice
T10284	1183-1189	SO_EXT:0001023	denotes	allele
T10285	1204-1208	NCBITaxon:10088	denotes	mice
T10286	1219-1223	GO_EXT:breeding	denotes	bred
T10287	1235-1244	SO_EXT:wild_type_entity_or_quality	denotes	wild type
T10288	1246-1247	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10289	1248-1249	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10290	1266-1267	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10291	1268-1269	SO_EXT:sequence_nullness_or_absence	denotes	-
T10292	1287-1288	SO_EXT:sequence_nullness_or_absence	denotes	-
T10293	1289-1290	SO_EXT:sequence_nullness_or_absence	denotes	-
T10294	1292-1299	NCBITaxon:33208	denotes	animals
T10295	1301-1305	NCBITaxon:10088	denotes	Mice
T10296	1352-1356	SO_EXT:0000147	denotes	Exon
T10297	1368-1373	CHEBI_SO_EXT:molecular_probe	denotes	probe
T10298	1374-1384	GO:0097617	denotes	hybridized
T10299	1400-1401	CHEBI_SO_EXT:base	denotes	b
T10300	1407-1427	SO_EXT:0000412	denotes	restriction fragment
T10301	1431-1432	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10302	1433-1434	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10303	1435-1439	NCBITaxon:10088	denotes	mice
T10304	1448-1449	CHEBI_SO_EXT:base	denotes	b
T10305	1467-1468	SO_EXT:sequence_nullness_or_absence	denotes	-
T10306	1469-1470	SO_EXT:sequence_nullness_or_absence	denotes	-
T10307	1471-1475	NCBITaxon:10088	denotes	mice
T10308	1511-1512	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10309	1513-1514	SO_EXT:sequence_nullness_or_absence	denotes	-
T10310	1515-1519	NCBITaxon:10088	denotes	mice
T10311	1531-1535	NCBITaxon:10088	denotes	Mice
T10312	1555-1557	PR_EXT:000006300	denotes	E2
T10313	1558-1566	SO_EXT:sequence_alteration_entity_or_process	denotes	mutation
T10314	1572-1576	GO:0007567	denotes	born
T10315	1604-1612	SO_EXT:genotype_or_entity_with_genotype	denotes	Genotype
T10316	1625-1629	NCBITaxon_UBERON_EXT:pup	denotes	pups
T10317	1643-1644	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10318	1645-1646	SO_EXT:sequence_nullness_or_absence	denotes	-
T10319	1650-1651	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10320	1652-1653	SO_EXT:sequence_nullness_or_absence	denotes	-
T10321	1654-1661	GO:0007618	denotes	matings
T10322	1676-1677	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10323	1678-1679	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10324	1681-1682	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10325	1683-1684	SO_EXT:sequence_nullness_or_absence	denotes	-
T10326	1690-1691	SO_EXT:sequence_nullness_or_absence	denotes	-
T10327	1692-1693	SO_EXT:sequence_nullness_or_absence	denotes	-
T10328	1694-1698	NCBITaxon:10088	denotes	mice
T10329	1770-1777	NCBITaxon:33208	denotes	animals
T10330	1803-1804	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10331	1805-1806	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10332	1822-1823	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10333	1824-1825	SO_EXT:sequence_nullness_or_absence	denotes	-
T10334	1845-1846	SO_EXT:sequence_nullness_or_absence	denotes	-
T10335	1847-1848	SO_EXT:sequence_nullness_or_absence	denotes	-
T10336	1860-1862	PR_EXT:000006300	denotes	E2
T10357	2345-2346	SO_EXT:sequence_nullness_or_absence	denotes	-
T10358	2347-2348	SO_EXT:sequence_nullness_or_absence	denotes	-
T10359	2349-2353	NCBITaxon_UBERON_EXT:pup	denotes	pups
T10360	2354-2358	GO:0016265	denotes	died
T10361	2378-2383	GO:0007567	denotes	birth
T10362	2411-2412	SO_EXT:sequence_nullness_or_absence	denotes	-
T10363	2413-2414	SO_EXT:sequence_nullness_or_absence	denotes	-
T10364	2415-2418	NCBITaxon_UBERON_EXT:pup	denotes	pup
T10365	2440-2445	GO:0007567	denotes	natal
T10366	2500-2503	NCBITaxon_UBERON_EXT:pup	denotes	pup
T12078	2517-2519	PR_EXT:000006300	denotes	E2
T12079	2530-2534	NCBITaxon:10088	denotes	mice
T12080	2589-2593	SO_EXT:0000704	denotes	gene
T12081	2625-2627	PR_EXT:000006300	denotes	E2
T12082	2628-2632	SO_EXT:sequence_nullness	denotes	null
T12083	2633-2639	SO_EXT:0001023	denotes	allele
T12084	2673-2678	UBERON:0002107	denotes	liver
T12085	2698-2703	GO:0007567	denotes	natal
T12086	2710-2715	NCBITaxon:10088	denotes	mouse
T12087	2716-2720	NCBITaxon_UBERON_EXT:pup	denotes	pups
T12088	2734-2735	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12089	2736-2737	SO_EXT:sequence_nullness_or_absence	denotes	-
T12090	2741-2742	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12091	2743-2744	SO_EXT:sequence_nullness_or_absence	denotes	-
T12092	2745-2751	GO:0007618	denotes	mating
T12093	2804-2805	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12094	2806-2807	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12095	2808-2812	NCBITaxon:10088	denotes	mice
T12096	2897-2898	SO_EXT:sequence_nullness_or_absence	denotes	-
T12097	2899-2900	SO_EXT:sequence_nullness_or_absence	denotes	-
T12098	2901-2906	NCBITaxon:10088	denotes	mouse
T12099	2907-2913	UBERON:0002107	denotes	livers
T12100	2945-2946	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12101	2947-2948	SO_EXT:sequence_nullness_or_absence	denotes	-
T12102	2949-2953	NCBITaxon:10088	denotes	mice
T12103	2999-3000	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12104	3001-3002	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12105	3045-3046	SO_EXT:sequence_nullness_or_absence	denotes	-
T12106	3047-3048	SO_EXT:sequence_nullness_or_absence	denotes	-
T12107	3049-3053	NCBITaxon:10088	denotes	mice
T12108	3086-3092	NCBITaxon:9606	denotes	humans
T12148	4695-4704	GO:0006537	denotes	glutamate
T12149	4710-4719	GO:0006542	denotes	glutamine
T12150	4686-4693	CHEBI_SO_EXT:alanine	denotes	alanine
T12151	4695-4704	CHEBI_EXT:glutamate	denotes	glutamate
T12152	4710-4719	CHEBI_SO_EXT:glutamine	denotes	glutamine
T12153	4746-4764	GO:0009081	denotes	metabolism of BCAA
T12154	4760-4764	CHEBI:22918	denotes	BCAA
T12155	4773-4777	NCBITaxon:10088	denotes	mice
T12156	4864-4869	UBERON:0000178	denotes	blood
T12157	4884-4891	CHEBI_SO_EXT:alanine	denotes	alanine
T12158	4893-4902	CHEBI_EXT:glutamate	denotes	glutamate
T12159	4908-4917	CHEBI_SO_EXT:glutamine	denotes	glutamine
T12160	4964-4975	CHEBI_SO_EXT:amino_acid	denotes	amino acids
T12161	4990-4994	NCBITaxon:10088	denotes	mice
T12162	5034-5035	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12163	5036-5037	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12164	5041-5042	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12165	5043-5044	SO_EXT:sequence_nullness_or_absence	denotes	-
T12166	5045-5049	NCBITaxon:10088	denotes	mice
T12167	5071-5082	CHEBI_SO_EXT:amino_acid	denotes	amino acids
T12168	5090-5091	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12169	5092-5093	SO_EXT:sequence_nullness_or_absence	denotes	-
T12170	5094-5098	NCBITaxon:10088	denotes	mice
T12171	5127-5128	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12172	5129-5130	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12173	5131-5135	NCBITaxon:10088	denotes	mice
T12174	5187-5192	UBERON:0000178	denotes	blood
T12175	5193-5200	CHEBI_SO_EXT:alanine	denotes	alanine
T12176	5226-5231	UBERON:0000178	denotes	blood
T12177	5232-5236	CHEBI:22918	denotes	BCAA
T12178	5342-5346	CHEBI:22918	denotes	BCAA
T12179	5347-5354	CHEBI_SO_EXT:alanine	denotes	alanine
T12180	5431-5435	CHEBI:22918	denotes	BCAA
T12181	5486-5496	CHEBI_SO_EXT:amino_acid	denotes	amino acid
T12182	5508-5512	CHEBI:22918	denotes	BCAA
T12183	5513-5520	CHEBI_SO_EXT:alanine	denotes	alanine
T12184	5565-5566	SO_EXT:sequence_nullness_or_absence	denotes	-
T12185	5567-5568	SO_EXT:sequence_nullness_or_absence	denotes	-
T12186	5569-5573	NCBITaxon_UBERON_EXT:pup	denotes	pups
T12187	5607-5608	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12188	5609-5610	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12189	5614-5615	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12190	5616-5617	SO_EXT:sequence_nullness_or_absence	denotes	-
T12191	5637-5642	UBERON:0000178	denotes	blood
T12192	5643-5653	CHEBI_SO_EXT:amino_acid	denotes	amino acid
T15719	15580-15584	CHEBI:22918	denotes	BCAA
T22750	3126-3137	CHEBI_EXT:biochemical	denotes	Biochemical
T22751	3175-3181	NCBITaxon:39107	denotes	murine
T22752	3198-3204	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T22753	3217-3222	UBERON:0002107	denotes	liver
T22754	3226-3233	PATO_UBERON_EXT:neonate_or_newborn	denotes	newborn
T22755	3234-3243	SO_EXT:wild_type_entity_or_quality	denotes	wild type
T22756	3253-3254	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22757	3255-3256	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22758	3273-3274	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22759	3275-3276	SO_EXT:sequence_nullness_or_absence	denotes	-
T22760	3295-3296	SO_EXT:sequence_nullness_or_absence	denotes	-
T22761	3297-3298	SO_EXT:sequence_nullness_or_absence	denotes	-
T22762	3309-3313	NCBITaxon:10088	denotes	mice
T22763	3315-3321	CHEBI_GO_SO_EXT:enzyme	denotes	Enzyme
T22764	3360-3361	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22765	3362-3363	SO_EXT:sequence_nullness_or_absence	denotes	-
T22766	3364-3369	UBERON:0002107	denotes	liver
T22767	3382-3383	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22768	3384-3385	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22769	3427-3428	SO_EXT:sequence_nullness_or_absence	denotes	-
T22770	3429-3430	SO_EXT:sequence_nullness_or_absence	denotes	-
T22771	3431-3436	UBERON:0002107	denotes	liver
T22772	3447-3451	CHEBI:22918	denotes	BCAA
T22773	3470-3475	UBERON:0000178	denotes	blood
T22774	3479-3483	NCBITaxon:10088	denotes	mice
T22775	3491-3495	CHEBI:22918	denotes	BCAA
T22776	3517-3524	CHEBI_SO_EXT:leucine	denotes	leucine
T22777	3526-3536	CHEBI_SO_EXT:isoleucine	denotes	isoleucine
T22778	3542-3548	CHEBI_SO_EXT:valine	denotes	valine
T22779	3556-3560	CHEBI:22918	denotes	BCAA
T22780	3579-3584	UBERON:0000178	denotes	blood
T22781	3590-3591	SO_EXT:sequence_nullness_or_absence	denotes	-
T22782	3592-3593	SO_EXT:sequence_nullness_or_absence	denotes	-
T22783	3594-3598	NCBITaxon:10088	denotes	mice
T22784	3639-3640	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22785	3641-3642	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22786	3647-3648	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22787	3649-3650	SO_EXT:sequence_nullness_or_absence	denotes	-
T22788	3670-3674	CHEBI:22918	denotes	BCAA
T22789	3678-3685	CHEBI_SO_EXT:alanine	denotes	alanine
T22790	3689-3694	UBERON:0000178	denotes	blood
T22791	3698-3702	NCBITaxon:10088	denotes	mice
T22792	3745-3746	SO_EXT:sequence_nullness_or_absence	denotes	-
T22793	3747-3748	SO_EXT:sequence_nullness_or_absence	denotes	-
T22794	3749-3753	NCBITaxon:10088	denotes	mice
T22795	3766-3767	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22796	3768-3769	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22797	3774-3775	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22798	3776-3777	SO_EXT:sequence_nullness_or_absence	denotes	-
T22799	3778-3782	NCBITaxon:10088	denotes	mice
T22800	3832-3836	NCBITaxon:10088	denotes	mice
T22801	3879-3880	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22802	3881-3882	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22803	3929-3930	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22804	3931-3932	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22805	3937-3938	SO_EXT:normal_or_wild_type_or_present	denotes	+
T22806	3939-3940	SO_EXT:sequence_nullness_or_absence	denotes	-
T24771	5769-5774	UBERON:0000178	denotes	blood
T24772	5775-5785	CHEBI_SO_EXT:amino_acid	denotes	amino acid
T24773	5927-5932	GO:0007567	denotes	birth
T10340	1962-1966	NCBITaxon:10088	denotes	mice
T10341	1967-1971	GO:0016265	denotes	died
T10342	1979-1995	UBERON:0012101	denotes	perinatal period
T10343	1983-1988	GO:0007567	denotes	natal
T10344	2019-2024	GO:0007567	denotes	birth
T12130	4316-4321	UBERON:0000178	denotes	blood
T12131	4338-4343	UBERON:0001088	denotes	urine
T12132	4371-4375	CHEBI:22918	denotes	BCAA
T10337	1863-1867	SO_EXT:0000704	denotes	gene
T10338	1895-1904	UBERON:0000922	denotes	embryonic
T10339	1895-1916	GO:0009790	denotes	embryonic development
T10345	2037-2041	NCBITaxon_UBERON_EXT:pup	denotes	pups
T10346	2076-2077	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10347	2078-2079	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10348	2084-2085	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10349	2086-2087	SO_EXT:sequence_nullness_or_absence	denotes	-
T10350	2140-2146	GO:0001967	denotes	suckle
T10351	2174-2179	GO:0007567	denotes	natal
T10352	2194-2195	SO_EXT:sequence_nullness_or_absence	denotes	-
T10353	2196-2197	SO_EXT:sequence_nullness_or_absence	denotes	-
T10354	2198-2202	NCBITaxon_UBERON_EXT:pup	denotes	pups
T10355	2301-2312	UBERON:0001004	denotes	respiratory
T10356	2313-2322	GO_EXT:biological_movement_or_translocation_process	denotes	movements
T12109	3983-3985	PR_EXT:000006300	denotes	E2
T12110	3995-4003	GO:0042571	denotes	antibody
T12111	4024-4026	PR_EXT:000006300	denotes	E2
T12112	4027-4034	CHEBI_PR_EXT:protein	denotes	protein
T12113	4042-4046	NCBITaxon:10088	denotes	mice
T12114	4093-4095	PR_EXT:000006300	denotes	E2
T12115	4096-4103	CHEBI_PR_EXT:protein	denotes	protein
T12116	4120-4125	UBERON:0002107	denotes	liver
T12117	4130-4139	UBERON:0000922	denotes	embryonic
T12118	4140-4151	CL:0000057	denotes	fibroblasts
T12119	4154-4155	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12120	4156-4157	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12121	4158-4162	NCBITaxon:10088	denotes	mice
T12122	4199-4201	PR_EXT:000006300	denotes	E2
T12123	4202-4209	CHEBI_PR_EXT:protein	denotes	protein
T12124	4235-4242	UBERON:0000479	denotes	tissues
T12125	4246-4247	SO_EXT:sequence_nullness_or_absence	denotes	-
T12126	4248-4249	SO_EXT:sequence_nullness_or_absence	denotes	-
T12127	4250-4254	NCBITaxon:10088	denotes	mice
T12128	4267-4269	PR_EXT:000006300	denotes	E2
T12129	4279-4283	NCBITaxon:10088	denotes	mice
T12133	4384-4391	CHEBI_SO_EXT:leucine	denotes	leucine
T12134	4393-4403	CHEBI_SO_EXT:isoleucine	denotes	isoleucine
T12135	4409-4415	CHEBI_SO_EXT:valine	denotes	valine
T12136	4438-4439	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12137	4440-4441	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12138	4463-4474	CHEBI_SO_EXT:amino_acid	denotes	amino acids
T12139	4529-4533	CHEBI:22918	denotes	BCAA
T12140	4570-4584	CHEBI_EXT:alloisoleucine	denotes	alloisoleucine
T12141	4616-4617	SO_EXT:sequence_nullness_or_absence	denotes	-
T12142	4618-4619	SO_EXT:sequence_nullness_or_absence	denotes	-
T12143	4625-4629	NCBITaxon:10088	denotes	mice
T12144	4635-4653	GO:0009081	denotes	metabolism of BCAA
T12145	4649-4653	CHEBI:22918	denotes	BCAA
T12146	4673-4693	GO:0006523	denotes	synthesis of alanine
T12147	4673-4685	_FRAGMENT	denotes	synthesis of
T15512	6997-6999	PR_EXT:000006300	denotes	E2
T15511	6991-6996	NCBITaxon:9606	denotes	human
T15510	6983-6990	GO:0010467	denotes	express
T15509	6962-6972	SO_EXT:transgenic_entity	denotes	transgenic
T15508	6909-6914	NCBITaxon:10088	denotes	mouse
T15507	6867-6876	GO_EXT:fatality_or_lethality	denotes	lethality
T15506	6858-6866	PATO_UBERON_EXT:neonate_or_newborn	denotes	neonatal
T15505	6849-6853	CHEBI:22918	denotes	BCAA
T15504	6818-6824	SO_EXT:sequence_rescue_process	denotes	rescue
T15503	6795-6805	SO_EXT:transgenic_entity	denotes	transgenic
T15502	6730-6736	NCBITaxon:39107	denotes	murine
T15501	6649-6658	SO_EXT:0000902	denotes	transgene
T15500	6646-6648	PR_EXT:000006300	denotes	E2
T15499	6640-6645	NCBITaxon:9606	denotes	human
T15498	6627-6637	GO:0010467	denotes	expressing
T15497	6622-6626	NCBITaxon:10088	denotes	mice
T15565	9571-9575	SO_EXT:sequence_copy_entity	denotes	copy
T15564	9561-9570	SO_EXT:0000902	denotes	transgene
T15563	9410-9420	SO_EXT:transgenic_entity	denotes	transgenic
T15562	9338-9353	SO_EXT:0000366	denotes	insertion sites
T15561	9328-9337	SO_EXT:0000902	denotes	transgene
T15560	9307-9314	NCBITaxon:33208	denotes	animals
T15559	9254-9262	GO_EXT:breeding	denotes	breeding
T15558	9214-9223	SO_EXT:0000902	denotes	transgene
T15557	9111-9121	SO_EXT:transgenic_entity	denotes	transgenic
T15556	9025-9034	SO_EXT:0000902	denotes	transgene
T15555	8963-8972	SO_EXT:0000902	denotes	transgene
T15554	8960-8962	PR_EXT:000006300	denotes	E2
T15553	8933-8937	NCBITaxon:10088	denotes	mice
T15552	8922-8932	SO_EXT:transgenic_entity	denotes	transgenic
T15551	8876-8886	GO:0045120	denotes	Pronuclear
T15550	8840-8841	NCBITaxon:10088	denotes	i
T15549	8834-8835	CHEBI_PR_EXT:protein	denotes	r
T15548	8831-8832	UBERON_EXT:muscle_structure_or_tissue	denotes	c
T15547	8825-8826	UBERON:0002113	denotes	d
T15546	8824-8825	NCBITaxon:10088	denotes	e
T15545	8823-8824	SO_EXT:transgenic_entity	denotes	c
T15544	8822-8823	NCBITaxon:9606	denotes	u
T15543	8821-8822	GO:0042571	denotes	d
T15542	8810-8811	UBERON:0002107	denotes	v
T15541	8807-8810	CHEBI_PR_EXT:protein	denotes	pre
T15624	12265-12272	CHEBI_SO_EXT:alanine	denotes	alanine
T15623	12260-12264	CHEBI:22918	denotes	BCAA
T15622	12247-12254	CHEBI_SO_EXT:alanine	denotes	alanine
T15621	12239-12243	CHEBI:22918	denotes	BCAA
T15620	12189-12199	CHEBI_SO_EXT:amino_acid	denotes	amino acid
T15619	12183-12188	UBERON:0000178	denotes	blood
T15618	12136-12137	GO_EXT:killing	denotes	c
T15617	12102-12103	UBERON:0000178	denotes	d
T15616	12100-12102	CHEBI_SO_EXT:alanine	denotes	an
T15615	12098-12099	CHEBI:22918	denotes	A
T15614	12095-12096	SO_EXT:genotype_or_entity_with_genotype	denotes	e
T15613	12092-12093	PR_EXT:000005308	denotes	L
T15612	10918-10922	NCBITaxon_UBERON_EXT:pup	denotes	pups
T15611	10868-10875	NCBITaxon:33208	denotes	animals
T15610	10861-10867	SO_EXT:sequence_rescue_process	denotes	rescue
T15609	10801-10807	GO_EXT:fatality_or_lethality	denotes	lethal
T15608	10792-10800	PATO_UBERON_EXT:neonate_or_newborn	denotes	neonatal
T15607	10781-10787	SO_EXT:sequence_rescue_process	denotes	rescue
T15606	10724-10726	PR_EXT:000006300	denotes	E2
T15605	10700-10706	GO_EXT:fatality_or_lethality	denotes	lethal
T15604	10691-10699	PATO_UBERON_EXT:neonate_or_newborn	denotes	neonatal
T15603	10678-10686	SO_EXT:sequence_rescue_process	denotes	rescuing
T15602	10634-10643	SO_EXT:0000902	denotes	transgene
T15601	10609-10617	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T15600	10602-10608	SO_EXT:sequence_rescue_process	denotes	rescue
R3288	T12148	T12147	_lexicallyChainedTo	glutamate,synthesis of
R3289	T12149	T12147	_lexicallyChainedTo	glutamine,synthesis of
R3834	T15647	T15646	_lexicallyChainedTo	protein,production of

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T24910	12842-12846	NCBITaxon:10088	denotes	mice
T24909	12692-12697	UBERON:0000178	denotes	blood
T24414	11967-11971	NCBITaxon:10088	denotes	mice
T24413	11932-11936	NCBITaxon:10088	denotes	mice
T24412	11873-11877	NCBITaxon:10088	denotes	mice
T24411	11835-11840	UBERON:0002107	denotes	liver
T24410	11603-11607	CHEBI:22918	denotes	BCAA
T24409	11544-11548	CHEBI:22918	denotes	BCAA
T24408	11497-11501	NCBITaxon:10088	denotes	mice
T24407	11488-11493	UBERON:0000178	denotes	blood
T24406	11469-11473	CHEBI:22918	denotes	BCAA
T24405	11365-11372	NCBITaxon:33208	denotes	animals
T24404	11245-11255	SO:0000902	denotes	transgenes
T24403	11242-11244	PR:000006300	denotes	E2
T24402	11226-11229	PR:000005308	denotes	LAP
T24401	11197-11199	PR:000006300	denotes	E2
T24400	11068-11072	NCBITaxon:10088	denotes	mice
T24399	10976-10982	NCBITaxon:10088	denotes	murine
T23587	8717-8725	GO:0042571	denotes	antibody
T23586	8711-8716	PR:000003676	denotes	actin
T23585	8669-8676	UBERON:0000479	denotes	tissues
T23584	8657-8659	PR:000006300	denotes	E2
T23583	8639-8648	SO:0000902	denotes	transgene
T23582	8581-8587	UBERON:0002113	denotes	kidney
T23581	8570-8575	UBERON:0000955	denotes	brain
T23580	8519-8523	NCBITaxon:10088	denotes	mice
T23579	8502-8504	PR:000006300	denotes	E2
T23578	8496-8501	NCBITaxon:9606	denotes	human
T23577	8482-8487	PR:000000084	denotes	c-myc
T23576	8463-8472	SO:0000902	denotes	transgene
T23575	8424-8432	GO:0042571	denotes	antibody
T23574	8414-8419	PR:000000084	denotes	c-myc
T23573	8387-8394	NCBITaxon:33208	denotes	animals
T23572	8361-8363	PR:000006300	denotes	E2
T15496	15896-15900	NCBITaxon:10088	denotes	mice
T15495	15718-15723	UBERON:0002107	denotes	liver
T15494	15663-15672	GO:0010467	denotes	expressed
T15493	15580-15584	CHEBI:22918	denotes	BCAA
T15492	15571-15575	NCBITaxon:10088	denotes	mice
T15491	15511-15515	GO:0016265	denotes	died
T15490	15453-15457	NCBITaxon:10088	denotes	mice
T15489	15283-15287	NCBITaxon:10088	denotes	mice
T15488	15244-15248	NCBITaxon:10088	denotes	mice
T15487	15166-15173	NCBITaxon:33208	denotes	animals
T15486	15042-15046	NCBITaxon:10088	denotes	mice
T15485	15007-15014	UBERON:0000479	denotes	tissues
T15484	14981-14983	PR:000006300	denotes	E2
T15483	14967-14977	GO:0010467	denotes	expression
T15473	14735-14746	PR:000006506	denotes	E3 subunits
T15472	14722-14727	NCBITaxon:10088	denotes	mouse
T15471	14707-14716	GO:0032991	denotes	complexed
T15470	14674-14676	PR:000006300	denotes	E2
T15469	14668-14673	NCBITaxon:9606	denotes	human
T15468	14620-14636	GO:0065003	denotes	subunit assembly
T15467	14581-14588	NCBITaxon:562	denotes	E. coli
T15466	14438-14468	GO:0045254	denotes	pyruvate dehydrogenase complex
T15465	14438-14446	CHEBI:15361	denotes	pyruvate
T15464	14266-14268	PR:000006300	denotes	E2
T15463	14260-14265	NCBITaxon:9606	denotes	human
T15462	14242-14251	SO:0000902	denotes	transgene
T15461	14201-14206	PR:000000084	denotes	c-myc
T15460	14136-14138	PR:000006300	denotes	E2
T15459	14118-14127	SO:0000902	denotes	transgene
T15458	14041-14043	PR:000006300	denotes	E2
T15457	13984-13988	NCBITaxon:10088	denotes	mice
T15456	13953-13958	UBERON:0002107	denotes	liver
T15455	13929-13931	PR:000006300	denotes	E2
T15454	13923-13928	NCBITaxon:10088	denotes	mouse
T15453	13879-13881	PR:000006300	denotes	E2
T15452	13873-13878	NCBITaxon:9606	denotes	human
T15451	13854-13858	NCBITaxon:10088	denotes	mice
T15450	13820-13824	NCBITaxon:10088	denotes	mice
T15449	13788-13792	NCBITaxon:10088	denotes	mice
T15448	13744-13746	PR:000006300	denotes	E2
T15447	13738-13743	NCBITaxon:9606	denotes	human
T15446	13686-13691	UBERON:0002107	denotes	liver
T15445	13584-13589	UBERON:0002107	denotes	liver
T15444	13516-13521	UBERON:0002107	denotes	liver
T15443	13502-13504	PR:000006300	denotes	E2
T15442	13496-13501	NCBITaxon:9606	denotes	human
T15441	13505-13512	GO:0006412	denotes	protein
T15440	13482-13495	_FRAGMENT	denotes	production of
T15439	13439-13449	GO:0010467	denotes	expression
T15438	13424-13429	UBERON:0002107	denotes	liver
T15437	13387-13389	PR:000006300	denotes	E2
T15436	13381-13386	NCBITaxon:9606	denotes	human
T15435	13356-13366	GO:0010467	denotes	expression
T15434	13327-13331	NCBITaxon:10088	denotes	mice
T15433	13319-13322	PR:000005308	denotes	LAP
T15432	12623-12627	NCBITaxon:10088	denotes	mice
T15380	7469-7477	SO:0000167	denotes	promoter
T15379	7431-7433	PR:000006300	denotes	E2
T15378	7425-7430	NCBITaxon:9606	denotes	human
T15377	7415-7422	GO:0010467	denotes	express
T15376	7389-7398	SO:0000902	denotes	transgene
T15375	7386-7388	PR:000006300	denotes	E2
T15374	7315-7324	SO:0000167	denotes	promoters
T15373	7301-7311	GO:0010467	denotes	expression
T15372	7229-7239	GO:0065007	denotes	controlled
T15371	7216-7228	CHEBI:27902	denotes	tetracycline
T15370	7198-7208	GO:0010467	denotes	expression
T15369	7183-7188	UBERON:0002107	denotes	liver
T15368	7145-7154	SO:0000902	denotes	transgene
T15367	7137-7140	PR:000005308	denotes	LAP
T15366	7112-7121	SO:0000902	denotes	transgene
T15365	7109-7111	PR:000006300	denotes	E2
T15364	7089-7098	SO:0000902	denotes	transgene
T15363	7081-7084	PR:000005308	denotes	LAP
T15362	7016-7018	PR:000006300	denotes	E2
T15361	7003-7008	UBERON:0002107	denotes	liver
T15360	6997-6999	PR:000006300	denotes	E2
T15359	6991-6996	NCBITaxon:9606	denotes	human
T15358	6983-6990	GO:0010467	denotes	express
T15357	6909-6914	NCBITaxon:10088	denotes	mouse
T15356	6849-6853	CHEBI:22918	denotes	BCAA
T15355	6730-6736	NCBITaxon:39107	denotes	murine
T15354	6649-6658	SO:0000902	denotes	transgene
T15353	6646-6648	PR:000006300	denotes	E2
T15352	6640-6645	NCBITaxon:9606	denotes	human
T15351	6627-6637	GO:0010467	denotes	expressing
T15350	6622-6626	NCBITaxon:10088	denotes	mice
T12076	6567-6569	PR:000006300	denotes	E2
T12075	6523-6529	NCBITaxon:9606	denotes	humans
T12074	6399-6408	GO:0008152	denotes	metabolic
T12073	6386-6391	UBERON:0001088	denotes	urine
T12072	6376-6381	UBERON:0000178	denotes	blood
T12071	6364-6368	CHEBI:22918	denotes	BCAA
T12070	6306-6308	PR:000006300	denotes	E2
T12069	6270-6274	NCBITaxon:10088	denotes	mice
T12068	6258-6260	PR:000006300	denotes	E2
T10166	23-25	PR:000006300	denotes	E2
T10167	26-30	SO:0000704	denotes	gene
T10168	40-44	NCBITaxon:10088	denotes	mice
T10169	55-57	PR:000006300	denotes	E2
T10170	67-71	NCBITaxon:10088	denotes	mice
T10171	81-85	SO:0000704	denotes	gene
T10172	99-104	NCBITaxon:10088	denotes	mouse
T10173	105-113	CL:0002322	denotes	ES cells
T10174	129-131	PR:000006300	denotes	E2
T10175	132-136	SO:0000704	denotes	gene
T10176	178-180	PR:000006300	denotes	E2
T10177	181-185	SO:0000704	denotes	gene
T10178	217-221	SO:0000704	denotes	gene
T10179	287-294	SO:0001026	denotes	genomic
T10180	321-333	SO:0000852	denotes	part of Exon
T10181	347-351	SO:0000147	denotes	Exon
T10182	405-412	CL:0002322	denotes	ES cell
T10183	502-543	SO:0000193	denotes	restriction fragment length polymorphisms
T10184	558-562	SO:0000704	denotes	gene
T10185	580-582	PR:000006300	denotes	E2
T10186	627-629	PR:000006300	denotes	E2
T10187	630-634	SO:0000147	denotes	Exon
T10188	699-709	GO:0097617	denotes	hybridizes
T10189	732-752	SO:0000412	denotes	restriction fragment
T10190	772-778	SO:0001023	denotes	allele
T10191	798-805	CL:0002322	denotes	ES cell
T10192	834-842	CL:0002322	denotes	ES cells
T10193	860-870	GO:0097617	denotes	hybridizes
T10194	888-908	SO:0000412	denotes	restriction fragment
T10195	1026-1034	CL:0002322	denotes	ES cells
T10196	1059-1070	UBERON:0000358	denotes	blastocysts
T10197	1091-1095	NCBITaxon:10088	denotes	mice
T10198	1129-1133	NCBITaxon:10088	denotes	mice
T10199	1183-1189	SO:0001023	denotes	allele
T10200	1204-1208	NCBITaxon:10088	denotes	mice
T10201	1292-1299	NCBITaxon:33208	denotes	animals
T10202	1301-1305	NCBITaxon:10088	denotes	Mice
T10203	1352-1356	SO:0000147	denotes	Exon
T10204	1374-1384	GO:0097617	denotes	hybridized
T10205	1407-1427	SO:0000412	denotes	restriction fragment
T10206	1435-1439	NCBITaxon:10088	denotes	mice
T10207	1471-1475	NCBITaxon:10088	denotes	mice
T10208	1515-1519	NCBITaxon:10088	denotes	mice
T10209	1531-1535	NCBITaxon:10088	denotes	Mice
T10210	1555-1557	PR:000006300	denotes	E2
T10211	1572-1576	GO:0007567	denotes	born
T10212	1654-1661	GO:0007618	denotes	matings
T10213	1694-1698	NCBITaxon:10088	denotes	mice
T10214	1770-1777	NCBITaxon:33208	denotes	animals
T10215	1860-1862	PR:000006300	denotes	E2
T10216	1863-1867	SO:0000704	denotes	gene
T10217	1895-1904	UBERON:0000922	denotes	embryonic
T10218	1895-1916	GO:0009790	denotes	embryonic development
T10219	1962-1966	NCBITaxon:10088	denotes	mice
T10220	1967-1971	GO:0016265	denotes	died
T10221	1979-1995	UBERON:0012101	denotes	perinatal period
T10222	1983-1988	GO:0007567	denotes	natal
T10223	2019-2024	GO:0007567	denotes	birth
T10224	2140-2146	GO:0001967	denotes	suckle
T10225	2174-2179	GO:0007567	denotes	natal
T10226	2301-2312	UBERON:0001004	denotes	respiratory
T10227	2354-2358	GO:0016265	denotes	died
T10228	2378-2383	GO:0007567	denotes	birth
T10229	2440-2445	GO:0007567	denotes	natal
T12013	2517-2519	PR:000006300	denotes	E2
T12014	2530-2534	NCBITaxon:10088	denotes	mice
T12015	2589-2593	SO:0000704	denotes	gene
T12016	2625-2627	PR:000006300	denotes	E2
T12017	2633-2639	SO:0001023	denotes	allele
T12018	2673-2678	UBERON:0002107	denotes	liver
T12019	2698-2703	GO:0007567	denotes	natal
T12020	2710-2715	NCBITaxon:10088	denotes	mouse
T12021	2745-2751	GO:0007618	denotes	mating
T12022	2808-2812	NCBITaxon:10088	denotes	mice
T12023	2901-2906	NCBITaxon:10088	denotes	mouse
T12024	2907-2913	UBERON:0002107	denotes	livers
T12025	2949-2953	NCBITaxon:10088	denotes	mice
T12026	3049-3053	NCBITaxon:10088	denotes	mice
T12027	3086-3092	NCBITaxon:9606	denotes	humans
T12028	3983-3985	PR:000006300	denotes	E2
T12029	3995-4003	GO:0042571	denotes	antibody
T12030	4024-4026	PR:000006300	denotes	E2
T12031	4042-4046	NCBITaxon:10088	denotes	mice
T12032	4093-4095	PR:000006300	denotes	E2
T12033	4120-4125	UBERON:0002107	denotes	liver
T12034	4130-4139	UBERON:0000922	denotes	embryonic
T12035	4140-4151	CL:0000057	denotes	fibroblasts
T12036	4158-4162	NCBITaxon:10088	denotes	mice
T12037	4199-4201	PR:000006300	denotes	E2
T12038	4235-4242	UBERON:0000479	denotes	tissues
T12039	4250-4254	NCBITaxon:10088	denotes	mice
T12040	4267-4269	PR:000006300	denotes	E2
T12041	4279-4283	NCBITaxon:10088	denotes	mice
T12042	4316-4321	UBERON:0000178	denotes	blood
T12043	4338-4343	UBERON:0001088	denotes	urine
T12044	4371-4375	CHEBI:22918	denotes	BCAA
T12045	4529-4533	CHEBI:22918	denotes	BCAA
T12046	4625-4629	NCBITaxon:10088	denotes	mice
T12047	4635-4653	GO:0009081	denotes	metabolism of BCAA
T12048	4649-4653	CHEBI:22918	denotes	BCAA
T12049	4673-4693	GO:0006523	denotes	synthesis of alanine
T12050	4673-4685	_FRAGMENT	denotes	synthesis of
T12051	4695-4704	GO:0006537	denotes	glutamate
T12052	4710-4719	GO:0006542	denotes	glutamine
T12053	4746-4764	GO:0009081	denotes	metabolism of BCAA
T12054	4760-4764	CHEBI:22918	denotes	BCAA
T12055	4773-4777	NCBITaxon:10088	denotes	mice
T12056	4864-4869	UBERON:0000178	denotes	blood
T12057	4990-4994	NCBITaxon:10088	denotes	mice
T12058	5045-5049	NCBITaxon:10088	denotes	mice
T12059	5094-5098	NCBITaxon:10088	denotes	mice
T12060	5131-5135	NCBITaxon:10088	denotes	mice
T12061	5187-5192	UBERON:0000178	denotes	blood
T12062	5226-5231	UBERON:0000178	denotes	blood
T12063	5232-5236	CHEBI:22918	denotes	BCAA
T12064	5342-5346	CHEBI:22918	denotes	BCAA
T12065	5431-5435	CHEBI:22918	denotes	BCAA
T12066	5508-5512	CHEBI:22918	denotes	BCAA
T12067	5637-5642	UBERON:0000178	denotes	blood
T12077	6579-6583	NCBITaxon:10088	denotes	mice
T15482	14902-14908	UBERON:0002113	denotes	kidney
T15481	14895-14900	UBERON:0000955	denotes	brain
T15480	14882-14891	SO:0000902	denotes	transgene
T15479	14879-14881	PR:000006300	denotes	E2
T15478	14861-14871	GO:0010467	denotes	expression
T15477	14787-14789	PR:000006300	denotes	E2
T15476	14781-14786	NCBITaxon:10088	denotes	mouse
T15475	14754-14756	PR:000006300	denotes	E2
T15474	14748-14753	NCBITaxon:9606	denotes	Human
T22732	3175-3181	NCBITaxon:39107	denotes	murine
T22733	3217-3222	UBERON:0002107	denotes	liver
T22734	3309-3313	NCBITaxon:10088	denotes	mice
T22735	3364-3369	UBERON:0002107	denotes	liver
T22736	3431-3436	UBERON:0002107	denotes	liver
T22737	3447-3451	CHEBI:22918	denotes	BCAA
T22738	3470-3475	UBERON:0000178	denotes	blood
T22739	3479-3483	NCBITaxon:10088	denotes	mice
T22740	3491-3495	CHEBI:22918	denotes	BCAA
T22741	3556-3560	CHEBI:22918	denotes	BCAA
T22742	3579-3584	UBERON:0000178	denotes	blood
T22743	3594-3598	NCBITaxon:10088	denotes	mice
T22744	3670-3674	CHEBI:22918	denotes	BCAA
T22745	3689-3694	UBERON:0000178	denotes	blood
T22746	3698-3702	NCBITaxon:10088	denotes	mice
T22747	3749-3753	NCBITaxon:10088	denotes	mice
T22748	3778-3782	NCBITaxon:10088	denotes	mice
T22749	3832-3836	NCBITaxon:10088	denotes	mice
T23571	8355-8360	NCBITaxon:10088	denotes	mouse
T23570	8307-8314	NCBITaxon:33208	denotes	animals
T23569	8247-8251	NCBITaxon:10088	denotes	mice
T23568	8211-8213	PR:000006300	denotes	E2
T23567	8205-8210	NCBITaxon:9606	denotes	human
T23566	8175-8179	NCBITaxon:10088	denotes	mice
T23565	8136-8141	UBERON:0002107	denotes	liver
T23564	8122-8124	PR:000006300	denotes	E2
T23563	8088-8092	NCBITaxon:10088	denotes	mice
T23562	8000-8024	SO:0000610	denotes	polyadenylation sequence
T23561	8000-8015	GO:0043631	denotes	polyadenylation
T23560	7987-7991	NCBITaxon:10633	denotes	SV40
T23559	7964-7969	PR:000000084	denotes	c-myc
T23558	7934-7936	PR:000006300	denotes	E2
T23557	7928-7933	NCBITaxon:9606	denotes	human
T23556	7904-7910	SO:0000188	denotes	intron
T23555	7882-7890	SO:0000167	denotes	promoter
T23554	7831-7843	CHEBI:27902	denotes	tetracycline
T23553	7808-7817	SO:0000902	denotes	transgene
T23552	7805-7807	PR:000006300	denotes	E2
T23551	7787-7795	SO:0000167	denotes	promoter
T23550	7783-7786	PR:000005308	denotes	LAP
T23549	7768-7773	UBERON:0002107	denotes	liver
T23548	7727-7737	GO:0065007	denotes	controlled
T23547	7714-7726	CHEBI:27902	denotes	tetracycline
T23546	7702-7709	GO:0010467	denotes	express
T23545	7697-7701	NCBITaxon:10088	denotes	mice
T23544	7649-7653	NCBITaxon:10088	denotes	mice
T23543	7630-7633	PR:000005308	denotes	LAP
T23542	7626-7628	PR:000006300	denotes	E2
T23541	7620-7625	NCBITaxon:9606	denotes	human
T23540	7612-7619	GO:0010467	denotes	express
T23539	7602-7606	NCBITaxon:10088	denotes	mice
T23538	7524-7529	NCBITaxon:10088	denotes	mouse
T24769	5769-5774	UBERON:0000178	denotes	blood
T24770	5927-5932	GO:0007567	denotes	birth
T15423	12102-12103	UBERON:0000178	denotes	d
T15422	12098-12099	CHEBI:22918	denotes	A
T15421	12092-12093	PR:000005308	denotes	L
T15420	10868-10875	NCBITaxon:33208	denotes	animals
T15419	10724-10726	PR:000006300	denotes	E2
T15418	10634-10643	SO:0000902	denotes	transgene
T15417	10503-10507	NCBITaxon:10088	denotes	mice
T15416	10322-10326	NCBITaxon:10088	denotes	mice
T15415	10270-10280	SO:0000902	denotes	transgenes
T15414	10236-10238	PR:000006300	denotes	E2
T15413	10185-10192	NCBITaxon:33208	denotes	animals
T15412	10020-10030	SO:0000902	denotes	transgenes
T15411	9962-9964	PR:000006300	denotes	E2
T15410	9943-9952	SO:0000902	denotes	transgene
T15409	9940-9942	PR:000006300	denotes	E2
T15408	9921-9930	SO:0000902	denotes	transgene
T15407	9913-9916	PR:000005308	denotes	LAP
T15406	9882-9886	NCBITaxon:10088	denotes	mice
T15405	9819-9821	PR:000006300	denotes	E2
T15404	9779-9788	SO:0000902	denotes	transgene
T15403	9771-9774	PR:000005308	denotes	LAP
T15402	9739-9743	NCBITaxon:10088	denotes	mice
T15401	9669-9673	NCBITaxon:10088	denotes	mice
T15400	9666-9668	PR:000006300	denotes	E2
T15399	9624-9628	UBERON:0002415	denotes	tail
T15398	9561-9570	SO:0000902	denotes	transgene
T15397	9338-9353	SO:0000366	denotes	insertion sites
T15396	9328-9337	SO:0000902	denotes	transgene
T15395	9307-9314	NCBITaxon:33208	denotes	animals
T15394	9214-9223	SO:0000902	denotes	transgene
T15393	9025-9034	SO:0000902	denotes	transgene
T15392	8963-8972	SO:0000902	denotes	transgene
T15391	8960-8962	PR:000006300	denotes	E2
T15390	8933-8937	NCBITaxon:10088	denotes	mice
T15389	8876-8886	GO:0045120	denotes	Pronuclear
T15388	8840-8841	NCBITaxon:10088	denotes	i
T15387	8825-8826	UBERON:0002113	denotes	d
T15386	8824-8825	NCBITaxon:10088	denotes	e
T15385	8822-8823	NCBITaxon:9606	denotes	u
T15384	8821-8822	GO:0042571	denotes	d
T15383	8810-8811	UBERON:0002107	denotes	v
T15382	8797-8801	NCBITaxon:10088	denotes	mice
T15381	8789-8792	PR:000005308	denotes	LAP
T15431	12587-12591	NCBITaxon:10088	denotes	mice
T15430	12514-12519	UBERON:0000178	denotes	Blood
T15429	12444-12448	NCBITaxon:10088	denotes	mice
T15428	12405-12409	CHEBI:22918	denotes	BCAA
T15427	12284-12288	NCBITaxon:10088	denotes	mice
T15426	12260-12264	CHEBI:22918	denotes	BCAA
T15425	12239-12243	CHEBI:22918	denotes	BCAA
T15424	12183-12188	UBERON:0000178	denotes	blood
R3286	T12051	T12050	_lexicallyChainedTo	glutamate,synthesis of
R3287	T12052	T12050	_lexicallyChainedTo	glutamine,synthesis of
R3833	T15441	T15440	_lexicallyChainedTo	protein,production of

PMC:1448208 / 16335-32301 JSONTXT

Annnotations TAB JSON ListView MergeView

craft-sa-dev

craft-ca-core-ex-dev

craft-ca-core-dev

PMC:1448208 / 16335-32301 JSON TXT