Id |
Subject |
Object |
Predicate |
Lexical cue |
T8817 |
0-7 |
NNP |
denotes |
Western |
T8818 |
8-12 |
NN |
denotes |
blot |
T8819 |
13-21 |
NN |
denotes |
analysis |
T8820 |
21-164 |
sentence |
denotes |
Protein extracts were isolated from homogenized liver (freshly harvested and flash frozen) of wildtype, Line 525A, and Line A transgenic mice. |
T8821 |
22-29 |
NN |
denotes |
Protein |
T8822 |
30-38 |
NNS |
denotes |
extracts |
T8823 |
44-52 |
VBN |
denotes |
isolated |
T8824 |
39-43 |
VBD |
denotes |
were |
T8825 |
53-57 |
IN |
denotes |
from |
T8826 |
58-69 |
VBN |
denotes |
homogenized |
T8827 |
70-75 |
NN |
denotes |
liver |
T8828 |
76-77 |
-LRB- |
denotes |
( |
T8829 |
77-84 |
RB |
denotes |
freshly |
T8830 |
85-94 |
VBN |
denotes |
harvested |
T8831 |
95-98 |
CC |
denotes |
and |
T8832 |
99-104 |
NN |
denotes |
flash |
T8833 |
105-111 |
VBN |
denotes |
frozen |
T8834 |
111-112 |
-RRB- |
denotes |
) |
T8835 |
113-115 |
IN |
denotes |
of |
T8836 |
116-124 |
NN |
denotes |
wildtype |
T8837 |
148-158 |
JJ |
denotes |
transgenic |
T8838 |
124-126 |
, |
denotes |
, |
T8839 |
126-130 |
NN |
denotes |
Line |
T8840 |
131-135 |
NN |
denotes |
525A |
T8841 |
135-137 |
, |
denotes |
, |
T8842 |
137-140 |
CC |
denotes |
and |
T8843 |
141-145 |
NN |
denotes |
Line |
T8844 |
146-147 |
NN |
denotes |
A |
T8845 |
159-163 |
NNS |
denotes |
mice |
T8846 |
163-164 |
. |
denotes |
. |
T8847 |
164-351 |
sentence |
denotes |
Protein (25 μg per sample) was analyzed by electrophoresis on a 10% SDS-PAGE Ready Gel (Bio-Rad, Hercules, CA) and transferred to PVDF membrane (Sequi-Blot; Bio-Rad) via electroblotting. |
T8848 |
165-172 |
NN |
denotes |
Protein |
T8849 |
196-204 |
VBN |
denotes |
analyzed |
T8850 |
173-174 |
-LRB- |
denotes |
( |
T8851 |
177-179 |
NN |
denotes |
μg |
T8852 |
174-176 |
CD |
denotes |
25 |
T8853 |
180-183 |
IN |
denotes |
per |
T8854 |
184-190 |
NN |
denotes |
sample |
T8855 |
190-191 |
-RRB- |
denotes |
) |
T8856 |
192-195 |
VBD |
denotes |
was |
T8857 |
205-207 |
IN |
denotes |
by |
T8858 |
208-223 |
NN |
denotes |
electrophoresis |
T8859 |
224-226 |
IN |
denotes |
on |
T8860 |
227-228 |
DT |
denotes |
a |
T8861 |
248-251 |
NN |
denotes |
Gel |
T8862 |
229-231 |
CD |
denotes |
10 |
T8863 |
231-232 |
NN |
denotes |
% |
T8864 |
233-236 |
NN |
denotes |
SDS |
T8865 |
237-241 |
NN |
denotes |
PAGE |
T8866 |
236-237 |
HYPH |
denotes |
- |
T8867 |
242-247 |
JJ |
denotes |
Ready |
T8868 |
252-253 |
-LRB- |
denotes |
( |
T8869 |
257-260 |
NNP |
denotes |
Rad |
T8870 |
253-256 |
NNP |
denotes |
Bio |
T8871 |
256-257 |
HYPH |
denotes |
- |
T8872 |
260-262 |
, |
denotes |
, |
T8873 |
262-270 |
NNP |
denotes |
Hercules |
T8874 |
270-272 |
, |
denotes |
, |
T8875 |
272-274 |
NNP |
denotes |
CA |
T8876 |
274-275 |
-RRB- |
denotes |
) |
T8877 |
276-279 |
CC |
denotes |
and |
T8878 |
280-291 |
VBN |
denotes |
transferred |
T8879 |
292-294 |
IN |
denotes |
to |
T8880 |
295-299 |
NN |
denotes |
PVDF |
T8881 |
300-308 |
NN |
denotes |
membrane |
T8882 |
309-310 |
-LRB- |
denotes |
( |
T8883 |
326-329 |
NNP |
denotes |
Rad |
T8884 |
310-315 |
NNP |
denotes |
Sequi |
T8885 |
316-320 |
NNP |
denotes |
Blot |
T8886 |
315-316 |
HYPH |
denotes |
- |
T8887 |
320-321 |
: |
denotes |
; |
T8888 |
322-325 |
NNP |
denotes |
Bio |
T8889 |
325-326 |
HYPH |
denotes |
- |
T8890 |
329-330 |
-RRB- |
denotes |
) |
T8891 |
331-334 |
IN |
denotes |
via |
T8892 |
335-350 |
NN |
denotes |
electroblotting |
T8893 |
350-351 |
. |
denotes |
. |
T8894 |
351-560 |
sentence |
denotes |
All blots were probed for E2 protein using polyclonal rabbit E2 antisera (1:5,000), which detects both mouse (~47 kD) and human (~54 kD) E2 subunits ([30]; gift from Dr. Susan Hutson, Wake Forest University). |
T8895 |
352-355 |
DT |
denotes |
All |
T8896 |
356-361 |
NNS |
denotes |
blots |
T8897 |
367-373 |
VBN |
denotes |
probed |
T8898 |
362-366 |
VBD |
denotes |
were |
T8899 |
374-377 |
IN |
denotes |
for |
T8900 |
378-380 |
NN |
denotes |
E2 |
T8901 |
381-388 |
NN |
denotes |
protein |
T8902 |
389-394 |
VBG |
denotes |
using |
T8903 |
395-405 |
JJ |
denotes |
polyclonal |
T8904 |
416-424 |
NNS |
denotes |
antisera |
T8905 |
406-412 |
NN |
denotes |
rabbit |
T8906 |
413-415 |
NN |
denotes |
E2 |
T8907 |
425-426 |
-LRB- |
denotes |
( |
T8908 |
428-433 |
CD |
denotes |
5,000 |
T8909 |
426-427 |
CD |
denotes |
1 |
T8910 |
427-428 |
SYM |
denotes |
: |
T8911 |
433-434 |
-RRB- |
denotes |
) |
T8912 |
434-436 |
, |
denotes |
, |
T8913 |
436-441 |
WDT |
denotes |
which |
T8914 |
442-449 |
VBZ |
denotes |
detects |
T8915 |
450-454 |
CC |
denotes |
both |
T8916 |
492-500 |
NNS |
denotes |
subunits |
T8917 |
455-460 |
NN |
denotes |
mouse |
T8918 |
461-462 |
-LRB- |
denotes |
( |
T8919 |
466-468 |
NN |
denotes |
kD |
T8920 |
462-463 |
SYM |
denotes |
~ |
T8921 |
463-465 |
CD |
denotes |
47 |
T8922 |
468-469 |
-RRB- |
denotes |
) |
T8923 |
470-473 |
CC |
denotes |
and |
T8924 |
474-479 |
JJ |
denotes |
human |
T8925 |
480-481 |
-LRB- |
denotes |
( |
T8926 |
485-487 |
NN |
denotes |
kD |
T8927 |
481-482 |
SYM |
denotes |
~ |
T8928 |
482-484 |
CD |
denotes |
54 |
T8929 |
487-488 |
-RRB- |
denotes |
) |
T8930 |
489-491 |
NN |
denotes |
E2 |
T8931 |
501-502 |
-LRB- |
denotes |
( |
T8932 |
508-512 |
NN |
denotes |
gift |
T8933 |
502-503 |
-LRB- |
denotes |
[ |
T8934 |
503-505 |
CD |
denotes |
30 |
T8935 |
505-506 |
-RRB- |
denotes |
] |
T8936 |
506-507 |
: |
denotes |
; |
T8937 |
513-517 |
IN |
denotes |
from |
T8938 |
518-521 |
NNP |
denotes |
Dr. |
T8939 |
528-534 |
NNP |
denotes |
Hutson |
T8940 |
522-527 |
NNP |
denotes |
Susan |
T8941 |
534-536 |
, |
denotes |
, |
T8942 |
536-540 |
NNP |
denotes |
Wake |
T8943 |
541-547 |
NNP |
denotes |
Forest |
T8944 |
548-558 |
NNP |
denotes |
University |
T8945 |
558-559 |
-RRB- |
denotes |
) |
T8946 |
559-560 |
. |
denotes |
. |
T8947 |
560-671 |
sentence |
denotes |
Blots were re-probed with a rabbit anti-c-myc tag antibody (1:10,000; abCam, Cambridge, MA; cat.# ab9106-100). |
T8948 |
561-566 |
NNS |
denotes |
Blots |
T8949 |
572-581 |
VBN |
denotes |
re-probed |
T8950 |
567-571 |
VBD |
denotes |
were |
T8951 |
582-586 |
IN |
denotes |
with |
T8952 |
587-588 |
DT |
denotes |
a |
T8953 |
611-619 |
NN |
denotes |
antibody |
T8954 |
589-595 |
NN |
denotes |
rabbit |
T8955 |
596-602 |
JJ |
denotes |
anti-c |
T8956 |
603-606 |
NN |
denotes |
myc |
T8957 |
602-603 |
HYPH |
denotes |
- |
T8958 |
607-610 |
NN |
denotes |
tag |
T8959 |
620-621 |
-LRB- |
denotes |
( |
T8960 |
659-665 |
NN |
denotes |
ab9106 |
T8961 |
621-622 |
CD |
denotes |
1 |
T8962 |
623-629 |
CD |
denotes |
10,000 |
T8963 |
622-623 |
SYM |
denotes |
: |
T8964 |
629-630 |
: |
denotes |
; |
T8965 |
631-636 |
NNP |
denotes |
abCam |
T8966 |
636-638 |
, |
denotes |
, |
T8967 |
638-647 |
NNP |
denotes |
Cambridge |
T8968 |
647-649 |
, |
denotes |
, |
T8969 |
649-651 |
NNP |
denotes |
MA |
T8970 |
651-652 |
: |
denotes |
; |
T8971 |
653-657 |
NN |
denotes |
cat. |
T8972 |
657-658 |
SYM |
denotes |
# |
T8973 |
665-666 |
HYPH |
denotes |
- |
T8974 |
666-669 |
CD |
denotes |
100 |
T8975 |
669-670 |
-RRB- |
denotes |
) |
T8976 |
670-671 |
. |
denotes |
. |
T8977 |
671-802 |
sentence |
denotes |
Blots were also re-probed with an antibody for β-actin (43 kD; 1:10,000; abCam; cat.# ab8227-50) to allow for loading comparisons. |
T8978 |
672-677 |
NNS |
denotes |
Blots |
T8979 |
688-697 |
VBN |
denotes |
re-probed |
T8980 |
678-682 |
VBD |
denotes |
were |
T8981 |
683-687 |
RB |
denotes |
also |
T8982 |
698-702 |
IN |
denotes |
with |
T8983 |
703-705 |
DT |
denotes |
an |
T8984 |
706-714 |
NN |
denotes |
antibody |
T8985 |
715-718 |
IN |
denotes |
for |
T8986 |
719-720 |
NN |
denotes |
β |
T8987 |
721-726 |
NN |
denotes |
actin |
T8988 |
720-721 |
HYPH |
denotes |
- |
T8989 |
727-728 |
-LRB- |
denotes |
( |
T8990 |
758-764 |
NN |
denotes |
ab8227 |
T8991 |
728-730 |
CD |
denotes |
43 |
T8992 |
731-733 |
NN |
denotes |
kD |
T8993 |
733-734 |
: |
denotes |
; |
T8994 |
735-736 |
CD |
denotes |
1 |
T8995 |
737-743 |
CD |
denotes |
10,000 |
T8996 |
736-737 |
SYM |
denotes |
: |
T8997 |
743-744 |
: |
denotes |
; |
T8998 |
745-750 |
NN |
denotes |
abCam |
T8999 |
750-751 |
: |
denotes |
; |
T9000 |
752-756 |
NN |
denotes |
cat. |
T9001 |
756-757 |
SYM |
denotes |
# |
T9002 |
764-765 |
HYPH |
denotes |
- |
T9003 |
765-767 |
CD |
denotes |
50 |
T9004 |
767-768 |
-RRB- |
denotes |
) |
T9005 |
769-771 |
TO |
denotes |
to |
T9006 |
772-777 |
VB |
denotes |
allow |
T9007 |
778-781 |
IN |
denotes |
for |
T9008 |
782-789 |
NN |
denotes |
loading |
T9009 |
790-801 |
NNS |
denotes |
comparisons |
T9010 |
801-802 |
. |
denotes |
. |
T9011 |
802-1056 |
sentence |
denotes |
A goat anti-rabbit secondary antibody conjugated to horseradish peroxidase (1:10,000; Novus, Littleton, CO; cat.# NB730-H) was used for detection using the Western Lightning chemiluminescence reagent (Perkin Elmer, Boston, MA) and exposed to X-ray film. |
T9012 |
803-804 |
DT |
denotes |
A |
T9013 |
832-840 |
NN |
denotes |
antibody |
T9014 |
805-809 |
NN |
denotes |
goat |
T9015 |
810-821 |
JJ |
denotes |
anti-rabbit |
T9016 |
822-831 |
JJ |
denotes |
secondary |
T9017 |
930-934 |
VBN |
denotes |
used |
T9018 |
841-851 |
VBN |
denotes |
conjugated |
T9019 |
852-854 |
IN |
denotes |
to |
T9020 |
855-866 |
NN |
denotes |
horseradish |
T9021 |
867-877 |
NN |
denotes |
peroxidase |
T9022 |
878-879 |
-LRB- |
denotes |
( |
T9023 |
923-924 |
NN |
denotes |
H |
T9024 |
879-880 |
CD |
denotes |
1 |
T9025 |
881-887 |
CD |
denotes |
10,000 |
T9026 |
880-881 |
SYM |
denotes |
: |
T9027 |
887-888 |
: |
denotes |
; |
T9028 |
889-894 |
NNP |
denotes |
Novus |
T9029 |
894-896 |
, |
denotes |
, |
T9030 |
896-905 |
NNP |
denotes |
Littleton |
T9031 |
905-907 |
, |
denotes |
, |
T9032 |
907-909 |
NNP |
denotes |
CO |
T9033 |
909-910 |
: |
denotes |
; |
T9034 |
911-915 |
NN |
denotes |
cat. |
T9035 |
915-916 |
SYM |
denotes |
# |
T9036 |
917-922 |
NN |
denotes |
NB730 |
T9037 |
922-923 |
HYPH |
denotes |
- |
T9038 |
924-925 |
-RRB- |
denotes |
) |
T9039 |
926-929 |
VBD |
denotes |
was |
T9040 |
935-938 |
IN |
denotes |
for |
T9041 |
939-948 |
NN |
denotes |
detection |
T9042 |
949-954 |
VBG |
denotes |
using |
T9043 |
955-958 |
DT |
denotes |
the |
T9044 |
995-1002 |
NN |
denotes |
reagent |
T9045 |
959-966 |
NNP |
denotes |
Western |
T9046 |
967-976 |
NNP |
denotes |
Lightning |
T9047 |
977-994 |
NN |
denotes |
chemiluminescence |
T9048 |
1003-1004 |
-LRB- |
denotes |
( |
T9049 |
1011-1016 |
NNP |
denotes |
Elmer |
T9050 |
1004-1010 |
NNP |
denotes |
Perkin |
T9051 |
1016-1018 |
, |
denotes |
, |
T9052 |
1018-1024 |
NNP |
denotes |
Boston |
T9053 |
1024-1026 |
, |
denotes |
, |
T9054 |
1026-1028 |
NNP |
denotes |
MA |
T9055 |
1028-1029 |
-RRB- |
denotes |
) |
T9056 |
1030-1033 |
CC |
denotes |
and |
T9057 |
1034-1041 |
VBN |
denotes |
exposed |
T9058 |
1042-1044 |
IN |
denotes |
to |
T9059 |
1045-1050 |
NN |
denotes |
X-ray |
T9060 |
1051-1055 |
NN |
denotes |
film |
T9061 |
1055-1056 |
. |
denotes |
. |
R2508 |
T8817 |
T8818 |
compound |
Western,blot |
R2509 |
T8818 |
T8819 |
compound |
blot,analysis |
R2510 |
T8821 |
T8822 |
compound |
Protein,extracts |
R2511 |
T8822 |
T8823 |
nsubjpass |
extracts,isolated |
R2512 |
T8824 |
T8823 |
auxpass |
were,isolated |
R2513 |
T8825 |
T8823 |
prep |
from,isolated |
R2514 |
T8826 |
T8827 |
amod |
homogenized,liver |
R2515 |
T8827 |
T8825 |
pobj |
liver,from |
R2516 |
T8828 |
T8827 |
punct |
(,liver |
R2517 |
T8829 |
T8830 |
advmod |
freshly,harvested |
R2518 |
T8830 |
T8827 |
acl |
harvested,liver |
R2519 |
T8831 |
T8830 |
cc |
and,harvested |
R2520 |
T8832 |
T8833 |
dep |
flash,frozen |
R2521 |
T8833 |
T8830 |
conj |
frozen,harvested |
R2522 |
T8834 |
T8827 |
punct |
),liver |
R2523 |
T8835 |
T8827 |
prep |
of,liver |
R2524 |
T8836 |
T8837 |
npadvmod |
wildtype,transgenic |
R2525 |
T8837 |
T8845 |
amod |
transgenic,mice |
R2526 |
T8838 |
T8836 |
punct |
", ",wildtype |
R2527 |
T8839 |
T8840 |
compound |
Line,525A |
R2528 |
T8840 |
T8836 |
conj |
525A,wildtype |
R2529 |
T8841 |
T8840 |
punct |
", ",525A |
R2530 |
T8842 |
T8840 |
cc |
and,525A |
R2531 |
T8843 |
T8844 |
compound |
Line,A |
R2532 |
T8844 |
T8840 |
conj |
A,525A |
R2533 |
T8845 |
T8835 |
pobj |
mice,of |
R2534 |
T8846 |
T8823 |
punct |
.,isolated |
R2535 |
T8848 |
T8849 |
nsubjpass |
Protein,analyzed |
R2536 |
T8850 |
T8851 |
punct |
(,μg |
R2537 |
T8851 |
T8848 |
parataxis |
μg,Protein |
R2538 |
T8852 |
T8851 |
nummod |
25,μg |
R2539 |
T8853 |
T8851 |
prep |
per,μg |
R2540 |
T8854 |
T8853 |
pobj |
sample,per |
R2541 |
T8855 |
T8851 |
punct |
),μg |
R2542 |
T8856 |
T8849 |
auxpass |
was,analyzed |
R2543 |
T8857 |
T8849 |
prep |
by,analyzed |
R2544 |
T8858 |
T8857 |
pobj |
electrophoresis,by |
R2545 |
T8859 |
T8849 |
prep |
on,analyzed |
R2546 |
T8860 |
T8861 |
det |
a,Gel |
R2547 |
T8861 |
T8859 |
pobj |
Gel,on |
R2548 |
T8862 |
T8863 |
nummod |
10,% |
R2549 |
T8863 |
T8861 |
nmod |
%,Gel |
R2550 |
T8864 |
T8865 |
nmod |
SDS,PAGE |
R2551 |
T8865 |
T8861 |
nmod |
PAGE,Gel |
R2552 |
T8866 |
T8865 |
punct |
-,PAGE |
R2553 |
T8867 |
T8861 |
amod |
Ready,Gel |
R2554 |
T8868 |
T8869 |
punct |
(,Rad |
R2555 |
T8869 |
T8861 |
parataxis |
Rad,Gel |
R2556 |
T8870 |
T8869 |
compound |
Bio,Rad |
R2557 |
T8871 |
T8869 |
punct |
-,Rad |
R2558 |
T8872 |
T8869 |
punct |
", ",Rad |
R2559 |
T8873 |
T8869 |
npadvmod |
Hercules,Rad |
R2560 |
T8874 |
T8869 |
punct |
", ",Rad |
R2561 |
T8875 |
T8869 |
npadvmod |
CA,Rad |
R2562 |
T8876 |
T8869 |
punct |
),Rad |
R2563 |
T8877 |
T8849 |
cc |
and,analyzed |
R2564 |
T8878 |
T8849 |
conj |
transferred,analyzed |
R2565 |
T8879 |
T8878 |
prep |
to,transferred |
R2566 |
T8880 |
T8881 |
compound |
PVDF,membrane |
R2567 |
T8881 |
T8879 |
pobj |
membrane,to |
R2568 |
T8882 |
T8883 |
punct |
(,Rad |
R2569 |
T8883 |
T8881 |
parataxis |
Rad,membrane |
R2570 |
T8884 |
T8885 |
compound |
Sequi,Blot |
R2571 |
T8885 |
T8883 |
dep |
Blot,Rad |
R2572 |
T8886 |
T8885 |
punct |
-,Blot |
R2573 |
T8887 |
T8883 |
punct |
;,Rad |
R2574 |
T8888 |
T8883 |
compound |
Bio,Rad |
R2575 |
T8889 |
T8883 |
punct |
-,Rad |
R2576 |
T8890 |
T8883 |
punct |
),Rad |
R2577 |
T8891 |
T8878 |
prep |
via,transferred |
R2578 |
T8892 |
T8891 |
pobj |
electroblotting,via |
R2579 |
T8893 |
T8849 |
punct |
.,analyzed |
R2580 |
T8895 |
T8896 |
det |
All,blots |
R2581 |
T8896 |
T8897 |
nsubjpass |
blots,probed |
R2582 |
T8898 |
T8897 |
auxpass |
were,probed |
R2583 |
T8899 |
T8897 |
prep |
for,probed |
R2584 |
T8900 |
T8901 |
compound |
E2,protein |
R2585 |
T8901 |
T8899 |
pobj |
protein,for |
R2586 |
T8902 |
T8897 |
advcl |
using,probed |
R2587 |
T8903 |
T8904 |
amod |
polyclonal,antisera |
R2588 |
T8904 |
T8902 |
dobj |
antisera,using |
R2589 |
T8905 |
T8904 |
compound |
rabbit,antisera |
R2590 |
T8906 |
T8904 |
compound |
E2,antisera |
R2591 |
T8907 |
T8908 |
punct |
(,"5,000" |
R2592 |
T8908 |
T8904 |
parataxis |
"5,000",antisera |
R2593 |
T8909 |
T8908 |
nummod |
1,"5,000" |
R2594 |
T8910 |
T8908 |
punct |
:,"5,000" |
R2595 |
T8911 |
T8908 |
punct |
),"5,000" |
R2596 |
T8912 |
T8904 |
punct |
", ",antisera |
R2597 |
T8913 |
T8914 |
dep |
which,detects |
R2598 |
T8914 |
T8904 |
relcl |
detects,antisera |
R2599 |
T8915 |
T8916 |
preconj |
both,subunits |
R2600 |
T8916 |
T8914 |
dobj |
subunits,detects |
R2601 |
T8917 |
T8916 |
nmod |
mouse,subunits |
R2602 |
T8918 |
T8919 |
punct |
(,kD |
R2603 |
T8919 |
T8917 |
parataxis |
kD,mouse |
R2604 |
T8920 |
T8921 |
punct |
~,47 |
R2605 |
T8921 |
T8919 |
nummod |
47,kD |
R2606 |
T8922 |
T8919 |
punct |
),kD |
R2607 |
T8923 |
T8917 |
cc |
and,mouse |
R2608 |
T8924 |
T8917 |
conj |
human,mouse |
R2609 |
T8925 |
T8926 |
punct |
(,kD |
R2610 |
T8926 |
T8924 |
parataxis |
kD,human |
R2611 |
T8927 |
T8928 |
punct |
~,54 |
R2612 |
T8928 |
T8926 |
nummod |
54,kD |
R2613 |
T8929 |
T8926 |
punct |
),kD |
R2614 |
T8930 |
T8916 |
compound |
E2,subunits |
R2615 |
T8931 |
T8932 |
punct |
(,gift |
R2616 |
T8932 |
T8916 |
parataxis |
gift,subunits |
R2617 |
T8933 |
T8932 |
punct |
[,gift |
R2618 |
T8934 |
T8932 |
dep |
30,gift |
R2619 |
T8935 |
T8932 |
punct |
],gift |
R2620 |
T8936 |
T8932 |
punct |
;,gift |
R2621 |
T8937 |
T8932 |
prep |
from,gift |
R2622 |
T8938 |
T8939 |
compound |
Dr.,Hutson |
R2623 |
T8939 |
T8937 |
pobj |
Hutson,from |
R2624 |
T8940 |
T8939 |
compound |
Susan,Hutson |
R2625 |
T8941 |
T8939 |
punct |
", ",Hutson |
R2626 |
T8942 |
T8943 |
compound |
Wake,Forest |
R2627 |
T8943 |
T8944 |
compound |
Forest,University |
R2628 |
T8944 |
T8939 |
npadvmod |
University,Hutson |
R2629 |
T8945 |
T8932 |
punct |
),gift |
R2630 |
T8946 |
T8897 |
punct |
.,probed |
R2631 |
T8948 |
T8949 |
nsubjpass |
Blots,re-probed |
R2632 |
T8950 |
T8949 |
auxpass |
were,re-probed |
R2633 |
T8951 |
T8949 |
prep |
with,re-probed |
R2634 |
T8952 |
T8953 |
det |
a,antibody |
R2635 |
T8953 |
T8951 |
pobj |
antibody,with |
R2636 |
T8954 |
T8953 |
nmod |
rabbit,antibody |
R2637 |
T8955 |
T8956 |
amod |
anti-c,myc |
R2638 |
T8956 |
T8953 |
compound |
myc,antibody |
R2639 |
T8957 |
T8956 |
punct |
-,myc |
R2640 |
T8958 |
T8953 |
compound |
tag,antibody |
R2641 |
T8959 |
T8960 |
punct |
(,ab9106 |
R2642 |
T8960 |
T8953 |
parataxis |
ab9106,antibody |
R2643 |
T8961 |
T8962 |
nummod |
1,"10,000" |
R2644 |
T8962 |
T8960 |
dep |
"10,000",ab9106 |
R2645 |
T8963 |
T8962 |
punct |
:,"10,000" |
R2646 |
T8964 |
T8960 |
punct |
;,ab9106 |
R2647 |
T8965 |
T8960 |
dep |
abCam,ab9106 |
R2648 |
T8966 |
T8965 |
punct |
", ",abCam |
R2649 |
T8967 |
T8965 |
npadvmod |
Cambridge,abCam |
R2650 |
T8968 |
T8965 |
punct |
", ",abCam |
R2651 |
T8969 |
T8965 |
npadvmod |
MA,abCam |
R2652 |
T8970 |
T8960 |
punct |
;,ab9106 |
R2653 |
T8971 |
T8960 |
nmod |
cat.,ab9106 |
R2654 |
T8972 |
T8960 |
punct |
#,ab9106 |
R2655 |
T8973 |
T8960 |
punct |
-,ab9106 |
R2656 |
T8974 |
T8960 |
nummod |
100,ab9106 |
R2657 |
T8975 |
T8960 |
punct |
),ab9106 |
R2658 |
T8976 |
T8949 |
punct |
.,re-probed |
R2659 |
T8978 |
T8979 |
nsubjpass |
Blots,re-probed |
R2660 |
T8980 |
T8979 |
auxpass |
were,re-probed |
R2661 |
T8981 |
T8979 |
advmod |
also,re-probed |
R2662 |
T8982 |
T8979 |
prep |
with,re-probed |
R2663 |
T8983 |
T8984 |
det |
an,antibody |
R2664 |
T8984 |
T8982 |
pobj |
antibody,with |
R2665 |
T8985 |
T8984 |
prep |
for,antibody |
R2666 |
T8986 |
T8987 |
compound |
β,actin |
R2667 |
T8987 |
T8985 |
pobj |
actin,for |
R2668 |
T8988 |
T8987 |
punct |
-,actin |
R2669 |
T8989 |
T8990 |
punct |
(,ab8227 |
R2670 |
T8990 |
T8987 |
parataxis |
ab8227,actin |
R2671 |
T8991 |
T8992 |
nummod |
43,kD |
R2672 |
T8992 |
T8990 |
dep |
kD,ab8227 |
R2673 |
T8993 |
T8990 |
punct |
;,ab8227 |
R2674 |
T8994 |
T8995 |
nummod |
1,"10,000" |
R2675 |
T8995 |
T8990 |
dep |
"10,000",ab8227 |
R2676 |
T8996 |
T8995 |
punct |
:,"10,000" |
R2677 |
T8997 |
T8990 |
punct |
;,ab8227 |
R2678 |
T8998 |
T8990 |
dep |
abCam,ab8227 |
R2679 |
T8999 |
T8990 |
punct |
;,ab8227 |
R2680 |
T9000 |
T8990 |
nmod |
cat.,ab8227 |
R2681 |
T9001 |
T8990 |
punct |
#,ab8227 |
R2682 |
T9002 |
T8990 |
punct |
-,ab8227 |
R2683 |
T9003 |
T8990 |
nummod |
50,ab8227 |
R2684 |
T9004 |
T8990 |
punct |
),ab8227 |
R2685 |
T9005 |
T9006 |
aux |
to,allow |
R2686 |
T9006 |
T8979 |
advcl |
allow,re-probed |
R2687 |
T9007 |
T9006 |
prep |
for,allow |
R2688 |
T9008 |
T9009 |
compound |
loading,comparisons |
R2689 |
T9009 |
T9007 |
pobj |
comparisons,for |
R2690 |
T9010 |
T8979 |
punct |
.,re-probed |
R2691 |
T9012 |
T9013 |
det |
A,antibody |
R2692 |
T9013 |
T9017 |
nsubjpass |
antibody,used |
R2693 |
T9014 |
T9013 |
nmod |
goat,antibody |
R2694 |
T9015 |
T9013 |
amod |
anti-rabbit,antibody |
R2695 |
T9016 |
T9013 |
amod |
secondary,antibody |
R2696 |
T9018 |
T9013 |
acl |
conjugated,antibody |
R2697 |
T9019 |
T9018 |
prep |
to,conjugated |
R2698 |
T9020 |
T9021 |
compound |
horseradish,peroxidase |
R2699 |
T9021 |
T9019 |
pobj |
peroxidase,to |
R2700 |
T9022 |
T9023 |
punct |
(,H |
R2701 |
T9023 |
T9021 |
parataxis |
H,peroxidase |
R2702 |
T9024 |
T9025 |
nummod |
1,"10,000" |
R2703 |
T9025 |
T9023 |
dep |
"10,000",H |
R2704 |
T9026 |
T9025 |
punct |
:,"10,000" |
R2705 |
T9027 |
T9023 |
punct |
;,H |
R2706 |
T9028 |
T9023 |
dep |
Novus,H |
R2707 |
T9029 |
T9028 |
punct |
", ",Novus |
R2708 |
T9030 |
T9028 |
npadvmod |
Littleton,Novus |
R2709 |
T9031 |
T9028 |
punct |
", ",Novus |
R2710 |
T9032 |
T9028 |
npadvmod |
CO,Novus |
R2711 |
T9033 |
T9023 |
punct |
;,H |
R2712 |
T9034 |
T9023 |
nmod |
cat.,H |
R2713 |
T9035 |
T9023 |
punct |
#,H |
R2714 |
T9036 |
T9023 |
compound |
NB730,H |
R2715 |
T9037 |
T9023 |
punct |
-,H |
R2716 |
T9038 |
T9023 |
punct |
),H |
R2717 |
T9039 |
T9017 |
auxpass |
was,used |
R2718 |
T9040 |
T9017 |
prep |
for,used |
R2719 |
T9041 |
T9040 |
pobj |
detection,for |
R2720 |
T9042 |
T9017 |
advcl |
using,used |
R2721 |
T9043 |
T9044 |
det |
the,reagent |
R2722 |
T9044 |
T9042 |
dobj |
reagent,using |
R2723 |
T9045 |
T9046 |
compound |
Western,Lightning |
R2724 |
T9046 |
T9044 |
compound |
Lightning,reagent |
R2725 |
T9047 |
T9044 |
compound |
chemiluminescence,reagent |
R2726 |
T9048 |
T9049 |
punct |
(,Elmer |
R2727 |
T9049 |
T9044 |
parataxis |
Elmer,reagent |
R2728 |
T9050 |
T9049 |
compound |
Perkin,Elmer |
R2729 |
T9051 |
T9049 |
punct |
", ",Elmer |
R2730 |
T9052 |
T9049 |
npadvmod |
Boston,Elmer |
R2731 |
T9053 |
T9049 |
punct |
", ",Elmer |
R2732 |
T9054 |
T9049 |
npadvmod |
MA,Elmer |
R2733 |
T9055 |
T9049 |
punct |
),Elmer |
R2734 |
T9056 |
T9017 |
cc |
and,used |
R2735 |
T9057 |
T9017 |
conj |
exposed,used |
R2736 |
T9058 |
T9057 |
prep |
to,exposed |
R2737 |
T9059 |
T9060 |
compound |
X-ray,film |
R2738 |
T9060 |
T9058 |
pobj |
film,to |
R2739 |
T9061 |
T9017 |
punct |
.,used |