Id |
Subject |
Object |
Predicate |
Lexical cue |
T8105 |
0-5 |
NN |
denotes |
Assay |
T8106 |
6-8 |
IN |
denotes |
of |
T8107 |
9-14 |
NN |
denotes |
BCKDH |
T8108 |
15-23 |
NN |
denotes |
activity |
T8109 |
23-92 |
sentence |
denotes |
Livers were removed, frozen in liquid nitrogen, and stored at -80°C. |
T8110 |
24-30 |
NNS |
denotes |
Livers |
T8111 |
36-43 |
VBN |
denotes |
removed |
T8112 |
31-35 |
VBD |
denotes |
were |
T8113 |
43-45 |
, |
denotes |
, |
T8114 |
45-51 |
VBN |
denotes |
frozen |
T8115 |
52-54 |
IN |
denotes |
in |
T8116 |
55-61 |
JJ |
denotes |
liquid |
T8117 |
62-70 |
NN |
denotes |
nitrogen |
T8118 |
70-72 |
, |
denotes |
, |
T8119 |
72-75 |
CC |
denotes |
and |
T8120 |
76-82 |
VBN |
denotes |
stored |
T8121 |
83-85 |
IN |
denotes |
at |
T8122 |
86-87 |
HYPH |
denotes |
- |
T8123 |
87-89 |
CD |
denotes |
80 |
T8124 |
89-91 |
NN |
denotes |
°C |
T8125 |
91-92 |
. |
denotes |
. |
T8126 |
92-211 |
sentence |
denotes |
At the time of enzyme assay, livers were thawed, and homogenized (1:9, w/v) in 0.25 M sucrose, 10 mM Tris-HC1, pH 7.4. |
T8127 |
93-95 |
IN |
denotes |
At |
T8128 |
134-140 |
VBN |
denotes |
thawed |
T8129 |
96-99 |
DT |
denotes |
the |
T8130 |
100-104 |
NN |
denotes |
time |
T8131 |
105-107 |
IN |
denotes |
of |
T8132 |
108-114 |
NN |
denotes |
enzyme |
T8133 |
115-120 |
NN |
denotes |
assay |
T8134 |
120-122 |
, |
denotes |
, |
T8135 |
122-128 |
NNS |
denotes |
livers |
T8136 |
129-133 |
VBD |
denotes |
were |
T8137 |
140-142 |
, |
denotes |
, |
T8138 |
142-145 |
CC |
denotes |
and |
T8139 |
146-157 |
VBN |
denotes |
homogenized |
T8140 |
158-159 |
-LRB- |
denotes |
( |
T8141 |
166-167 |
NN |
denotes |
v |
T8142 |
159-160 |
CD |
denotes |
1 |
T8143 |
161-162 |
CD |
denotes |
9 |
T8144 |
160-161 |
SYM |
denotes |
: |
T8145 |
162-164 |
, |
denotes |
, |
T8146 |
164-165 |
NN |
denotes |
w |
T8147 |
165-166 |
HYPH |
denotes |
/ |
T8148 |
167-168 |
-RRB- |
denotes |
) |
T8149 |
169-171 |
IN |
denotes |
in |
T8150 |
172-176 |
CD |
denotes |
0.25 |
T8151 |
177-178 |
NN |
denotes |
M |
T8152 |
179-186 |
NN |
denotes |
sucrose |
T8153 |
186-188 |
, |
denotes |
, |
T8154 |
188-190 |
CD |
denotes |
10 |
T8155 |
191-193 |
NN |
denotes |
mM |
T8156 |
199-202 |
NN |
denotes |
HC1 |
T8157 |
194-198 |
NN |
denotes |
Tris |
T8158 |
198-199 |
HYPH |
denotes |
- |
T8159 |
202-204 |
, |
denotes |
, |
T8160 |
204-206 |
NN |
denotes |
pH |
T8161 |
207-210 |
CD |
denotes |
7.4 |
T8162 |
210-211 |
. |
denotes |
. |
T8163 |
211-347 |
sentence |
denotes |
Liver homogenates were centrifuged at 600 × g for 10 min at 4°C and the supernatant fraction was saved to determine the BCKDH activity. |
T8164 |
212-217 |
NN |
denotes |
Liver |
T8165 |
218-229 |
NNS |
denotes |
homogenates |
T8166 |
235-246 |
VBN |
denotes |
centrifuged |
T8167 |
230-234 |
VBD |
denotes |
were |
T8168 |
247-249 |
IN |
denotes |
at |
T8169 |
250-253 |
CD |
denotes |
600 |
T8170 |
256-257 |
NN |
denotes |
g |
T8171 |
254-255 |
SYM |
denotes |
× |
T8172 |
258-261 |
IN |
denotes |
for |
T8173 |
262-264 |
CD |
denotes |
10 |
T8174 |
265-268 |
NN |
denotes |
min |
T8175 |
269-271 |
IN |
denotes |
at |
T8176 |
272-273 |
CD |
denotes |
4 |
T8177 |
273-275 |
NN |
denotes |
°C |
T8178 |
276-279 |
CC |
denotes |
and |
T8179 |
280-283 |
DT |
denotes |
the |
T8180 |
296-304 |
NN |
denotes |
fraction |
T8181 |
284-295 |
NN |
denotes |
supernatant |
T8182 |
309-314 |
VBN |
denotes |
saved |
T8183 |
305-308 |
VBD |
denotes |
was |
T8184 |
315-317 |
TO |
denotes |
to |
T8185 |
318-327 |
VB |
denotes |
determine |
T8186 |
328-331 |
DT |
denotes |
the |
T8187 |
338-346 |
NN |
denotes |
activity |
T8188 |
332-337 |
NN |
denotes |
BCKDH |
T8189 |
346-347 |
. |
denotes |
. |
T8190 |
347-471 |
sentence |
denotes |
The use of tissue homogenates was necessitated by the limited availability of liver tissue, particularly from newborn pups. |
T8191 |
348-351 |
DT |
denotes |
The |
T8192 |
352-355 |
NN |
denotes |
use |
T8193 |
382-394 |
VBN |
denotes |
necessitated |
T8194 |
356-358 |
IN |
denotes |
of |
T8195 |
359-365 |
NN |
denotes |
tissue |
T8196 |
366-377 |
NNS |
denotes |
homogenates |
T8197 |
378-381 |
VBD |
denotes |
was |
T8198 |
395-397 |
IN |
denotes |
by |
T8199 |
398-401 |
DT |
denotes |
the |
T8200 |
410-422 |
NN |
denotes |
availability |
T8201 |
402-409 |
JJ |
denotes |
limited |
T8202 |
423-425 |
IN |
denotes |
of |
T8203 |
426-431 |
NN |
denotes |
liver |
T8204 |
432-438 |
NN |
denotes |
tissue |
T8205 |
438-440 |
, |
denotes |
, |
T8206 |
440-452 |
RB |
denotes |
particularly |
T8207 |
453-457 |
IN |
denotes |
from |
T8208 |
458-465 |
JJ |
denotes |
newborn |
T8209 |
466-470 |
NNS |
denotes |
pups |
T8210 |
470-471 |
. |
denotes |
. |
T8211 |
471-597 |
sentence |
denotes |
BCKDH activity was determined by measuring the release of 14CO2 from α-keto [1-14C] isocaproate as described previously [31]. |
T8212 |
472-477 |
NN |
denotes |
BCKDH |
T8213 |
478-486 |
NN |
denotes |
activity |
T8214 |
491-501 |
VBN |
denotes |
determined |
T8215 |
487-490 |
VBD |
denotes |
was |
T8216 |
502-504 |
IN |
denotes |
by |
T8217 |
505-514 |
VBG |
denotes |
measuring |
T8218 |
515-518 |
DT |
denotes |
the |
T8219 |
519-526 |
NN |
denotes |
release |
T8220 |
527-529 |
IN |
denotes |
of |
T8221 |
530-535 |
NN |
denotes |
14CO2 |
T8222 |
536-540 |
IN |
denotes |
from |
T8223 |
541-542 |
NN |
denotes |
α |
T8224 |
543-547 |
NN |
denotes |
keto |
T8225 |
542-543 |
HYPH |
denotes |
- |
T8226 |
556-567 |
NN |
denotes |
isocaproate |
T8227 |
548-549 |
-LRB- |
denotes |
[ |
T8228 |
549-550 |
CD |
denotes |
1 |
T8229 |
550-551 |
SYM |
denotes |
- |
T8230 |
551-554 |
NN |
denotes |
14C |
T8231 |
554-555 |
-RRB- |
denotes |
] |
T8232 |
568-570 |
IN |
denotes |
as |
T8233 |
571-580 |
VBN |
denotes |
described |
T8234 |
581-591 |
RB |
denotes |
previously |
T8235 |
592-593 |
-LRB- |
denotes |
[ |
T8236 |
593-595 |
CD |
denotes |
31 |
T8237 |
595-596 |
-RRB- |
denotes |
] |
T8238 |
596-597 |
. |
denotes |
. |
T8239 |
597-922 |
sentence |
denotes |
The complete reaction mixture contained (final volume 1 ml) 30 mM potassium phosphate buffer, pH 7.4, 0.20 mM α-ketoisocaproate, 0.40 mM CoASH, 0.40 mM thiamin pyrophosphate, 2 mM NAD+, 2 mM dithiothreitol, 5 mM Mg2+, approximately 250,000 DPM of α-keto [1-14C] isocaproate, and 0.10 ml of liver homogenate (2–3 mg protein). |
T8240 |
598-601 |
DT |
denotes |
The |
T8241 |
620-627 |
NN |
denotes |
mixture |
T8242 |
602-610 |
JJ |
denotes |
complete |
T8243 |
611-619 |
NN |
denotes |
reaction |
T8244 |
628-637 |
VBD |
denotes |
contained |
T8245 |
638-639 |
-LRB- |
denotes |
( |
T8246 |
645-651 |
NN |
denotes |
volume |
T8247 |
639-644 |
JJ |
denotes |
final |
T8248 |
652-653 |
CD |
denotes |
1 |
T8249 |
654-656 |
NN |
denotes |
ml |
T8250 |
656-657 |
-RRB- |
denotes |
) |
T8251 |
658-660 |
CD |
denotes |
30 |
T8252 |
661-663 |
NN |
denotes |
mM |
T8253 |
684-690 |
NN |
denotes |
buffer |
T8254 |
664-673 |
NN |
denotes |
potassium |
T8255 |
674-683 |
NN |
denotes |
phosphate |
T8256 |
690-692 |
, |
denotes |
, |
T8257 |
692-694 |
NN |
denotes |
pH |
T8258 |
695-698 |
CD |
denotes |
7.4 |
T8259 |
698-700 |
, |
denotes |
, |
T8260 |
700-704 |
CD |
denotes |
0.20 |
T8261 |
705-707 |
NN |
denotes |
mM |
T8262 |
710-725 |
NN |
denotes |
ketoisocaproate |
T8263 |
708-709 |
NN |
denotes |
α |
T8264 |
709-710 |
HYPH |
denotes |
- |
T8265 |
725-727 |
, |
denotes |
, |
T8266 |
727-731 |
CD |
denotes |
0.40 |
T8267 |
732-734 |
NN |
denotes |
mM |
T8268 |
735-740 |
NN |
denotes |
CoASH |
T8269 |
740-742 |
, |
denotes |
, |
T8270 |
742-746 |
CD |
denotes |
0.40 |
T8271 |
747-749 |
NN |
denotes |
mM |
T8272 |
758-771 |
NN |
denotes |
pyrophosphate |
T8273 |
750-757 |
NN |
denotes |
thiamin |
T8274 |
771-773 |
, |
denotes |
, |
T8275 |
773-774 |
CD |
denotes |
2 |
T8276 |
775-777 |
NN |
denotes |
mM |
T8277 |
778-781 |
NN |
denotes |
NAD |
T8278 |
781-782 |
SYM |
denotes |
+ |
T8279 |
782-784 |
, |
denotes |
, |
T8280 |
784-785 |
CD |
denotes |
2 |
T8281 |
786-788 |
NN |
denotes |
mM |
T8282 |
789-803 |
NN |
denotes |
dithiothreitol |
T8283 |
803-805 |
, |
denotes |
, |
T8284 |
805-806 |
CD |
denotes |
5 |
T8285 |
807-809 |
NN |
denotes |
mM |
T8286 |
810-813 |
NN |
denotes |
Mg2 |
T8287 |
813-814 |
SYM |
denotes |
+ |
T8288 |
814-816 |
, |
denotes |
, |
T8289 |
816-829 |
RB |
denotes |
approximately |
T8290 |
830-837 |
CD |
denotes |
250,000 |
T8291 |
838-841 |
NN |
denotes |
DPM |
T8292 |
842-844 |
IN |
denotes |
of |
T8293 |
845-846 |
NN |
denotes |
α |
T8294 |
847-851 |
NN |
denotes |
keto |
T8295 |
846-847 |
HYPH |
denotes |
- |
T8296 |
860-871 |
NN |
denotes |
isocaproate |
T8297 |
852-853 |
-LRB- |
denotes |
[ |
T8298 |
853-854 |
CD |
denotes |
1 |
T8299 |
854-855 |
SYM |
denotes |
- |
T8300 |
855-858 |
NN |
denotes |
14C |
T8301 |
858-859 |
-RRB- |
denotes |
] |
T8302 |
871-873 |
, |
denotes |
, |
T8303 |
873-876 |
CC |
denotes |
and |
T8304 |
877-881 |
CD |
denotes |
0.10 |
T8305 |
882-884 |
NN |
denotes |
ml |
T8306 |
885-887 |
IN |
denotes |
of |
T8307 |
888-893 |
NN |
denotes |
liver |
T8308 |
894-904 |
NN |
denotes |
homogenate |
T8309 |
905-906 |
-LRB- |
denotes |
( |
T8310 |
913-920 |
NN |
denotes |
protein |
T8311 |
906-907 |
CD |
denotes |
2 |
T8312 |
908-909 |
CD |
denotes |
3 |
T8313 |
907-908 |
SYM |
denotes |
– |
T8314 |
910-912 |
NN |
denotes |
mg |
T8315 |
920-921 |
-RRB- |
denotes |
) |
T8316 |
921-922 |
. |
denotes |
. |
T8317 |
922-1080 |
sentence |
denotes |
Assays were carried out for 15 min at 37°C, 14CO2 was trapped in hydroxide of Hyamine, and radioactivity was determined by liquid scintillation spectrometry. |
T8318 |
923-929 |
NNS |
denotes |
Assays |
T8319 |
935-942 |
VBN |
denotes |
carried |
T8320 |
930-934 |
VBD |
denotes |
were |
T8321 |
943-946 |
RP |
denotes |
out |
T8322 |
947-950 |
IN |
denotes |
for |
T8323 |
951-953 |
CD |
denotes |
15 |
T8324 |
954-957 |
NN |
denotes |
min |
T8325 |
958-960 |
IN |
denotes |
at |
T8326 |
961-963 |
CD |
denotes |
37 |
T8327 |
963-965 |
NN |
denotes |
°C |
T8328 |
965-967 |
, |
denotes |
, |
T8329 |
967-972 |
NN |
denotes |
14CO2 |
T8330 |
977-984 |
VBN |
denotes |
trapped |
T8331 |
973-976 |
VBD |
denotes |
was |
T8332 |
985-987 |
IN |
denotes |
in |
T8333 |
988-997 |
NN |
denotes |
hydroxide |
T8334 |
998-1000 |
IN |
denotes |
of |
T8335 |
1001-1008 |
NN |
denotes |
Hyamine |
T8336 |
1008-1010 |
, |
denotes |
, |
T8337 |
1010-1013 |
CC |
denotes |
and |
T8338 |
1014-1027 |
NN |
denotes |
radioactivity |
T8339 |
1032-1042 |
VBN |
denotes |
determined |
T8340 |
1028-1031 |
VBD |
denotes |
was |
T8341 |
1043-1045 |
IN |
denotes |
by |
T8342 |
1046-1052 |
JJ |
denotes |
liquid |
T8343 |
1053-1066 |
NN |
denotes |
scintillation |
T8344 |
1067-1079 |
NN |
denotes |
spectrometry |
T8345 |
1079-1080 |
. |
denotes |
. |
R2282 |
T8106 |
T8105 |
prep |
of,Assay |
R2283 |
T8107 |
T8108 |
compound |
BCKDH,activity |
R2284 |
T8108 |
T8106 |
pobj |
activity,of |
R2285 |
T8110 |
T8111 |
nsubjpass |
Livers,removed |
R2286 |
T8112 |
T8111 |
auxpass |
were,removed |
R2287 |
T8113 |
T8111 |
punct |
", ",removed |
R2288 |
T8114 |
T8111 |
conj |
frozen,removed |
R2289 |
T8115 |
T8114 |
prep |
in,frozen |
R2290 |
T8116 |
T8117 |
amod |
liquid,nitrogen |
R2291 |
T8117 |
T8115 |
pobj |
nitrogen,in |
R2292 |
T8118 |
T8114 |
punct |
", ",frozen |
R2293 |
T8119 |
T8114 |
cc |
and,frozen |
R2294 |
T8120 |
T8114 |
conj |
stored,frozen |
R2295 |
T8121 |
T8120 |
prep |
at,stored |
R2296 |
T8122 |
T8123 |
punct |
-,80 |
R2297 |
T8123 |
T8124 |
nummod |
80,°C |
R2298 |
T8124 |
T8121 |
pobj |
°C,at |
R2299 |
T8125 |
T8111 |
punct |
.,removed |
R2300 |
T8127 |
T8128 |
prep |
At,thawed |
R2301 |
T8129 |
T8130 |
det |
the,time |
R2302 |
T8130 |
T8127 |
pobj |
time,At |
R2303 |
T8131 |
T8130 |
prep |
of,time |
R2304 |
T8132 |
T8133 |
compound |
enzyme,assay |
R2305 |
T8133 |
T8131 |
pobj |
assay,of |
R2306 |
T8134 |
T8128 |
punct |
", ",thawed |
R2307 |
T8135 |
T8128 |
nsubjpass |
livers,thawed |
R2308 |
T8136 |
T8128 |
auxpass |
were,thawed |
R2309 |
T8137 |
T8128 |
punct |
", ",thawed |
R2310 |
T8138 |
T8128 |
cc |
and,thawed |
R2311 |
T8139 |
T8128 |
conj |
homogenized,thawed |
R2312 |
T8140 |
T8141 |
punct |
(,v |
R2313 |
T8141 |
T8139 |
parataxis |
v,homogenized |
R2314 |
T8142 |
T8143 |
nummod |
1,9 |
R2315 |
T8143 |
T8141 |
dep |
9,v |
R2316 |
T8144 |
T8143 |
punct |
:,9 |
R2317 |
T8145 |
T8141 |
punct |
", ",v |
R2318 |
T8146 |
T8141 |
compound |
w,v |
R2319 |
T8147 |
T8141 |
punct |
/,v |
R2320 |
T8148 |
T8141 |
punct |
),v |
R2321 |
T8149 |
T8139 |
prep |
in,homogenized |
R2322 |
T8150 |
T8151 |
nummod |
0.25,M |
R2323 |
T8151 |
T8152 |
compound |
M,sucrose |
R2324 |
T8152 |
T8149 |
pobj |
sucrose,in |
R2325 |
T8153 |
T8152 |
punct |
", ",sucrose |
R2326 |
T8154 |
T8155 |
nummod |
10,mM |
R2327 |
T8155 |
T8156 |
compound |
mM,HC1 |
R2328 |
T8156 |
T8152 |
appos |
HC1,sucrose |
R2329 |
T8157 |
T8156 |
compound |
Tris,HC1 |
R2330 |
T8158 |
T8156 |
punct |
-,HC1 |
R2331 |
T8159 |
T8156 |
punct |
", ",HC1 |
R2332 |
T8160 |
T8156 |
npadvmod |
pH,HC1 |
R2333 |
T8161 |
T8160 |
nummod |
7.4,pH |
R2334 |
T8162 |
T8128 |
punct |
.,thawed |
R2335 |
T8164 |
T8165 |
compound |
Liver,homogenates |
R2336 |
T8165 |
T8166 |
nsubjpass |
homogenates,centrifuged |
R2337 |
T8167 |
T8166 |
auxpass |
were,centrifuged |
R2338 |
T8168 |
T8166 |
prep |
at,centrifuged |
R2339 |
T8169 |
T8170 |
nummod |
600,g |
R2340 |
T8170 |
T8168 |
pobj |
g,at |
R2341 |
T8171 |
T8169 |
punct |
×,600 |
R2342 |
T8172 |
T8166 |
prep |
for,centrifuged |
R2343 |
T8173 |
T8174 |
nummod |
10,min |
R2344 |
T8174 |
T8172 |
pobj |
min,for |
R2345 |
T8175 |
T8166 |
prep |
at,centrifuged |
R2346 |
T8176 |
T8177 |
nummod |
4,°C |
R2347 |
T8177 |
T8175 |
pobj |
°C,at |
R2348 |
T8178 |
T8166 |
cc |
and,centrifuged |
R2349 |
T8179 |
T8180 |
det |
the,fraction |
R2350 |
T8180 |
T8182 |
nsubjpass |
fraction,saved |
R2351 |
T8181 |
T8180 |
compound |
supernatant,fraction |
R2352 |
T8182 |
T8166 |
conj |
saved,centrifuged |
R2353 |
T8183 |
T8182 |
auxpass |
was,saved |
R2354 |
T8184 |
T8185 |
aux |
to,determine |
R2355 |
T8185 |
T8182 |
advcl |
determine,saved |
R2356 |
T8186 |
T8187 |
det |
the,activity |
R2357 |
T8187 |
T8185 |
dobj |
activity,determine |
R2358 |
T8188 |
T8187 |
compound |
BCKDH,activity |
R2359 |
T8189 |
T8182 |
punct |
.,saved |
R2360 |
T8191 |
T8192 |
det |
The,use |
R2361 |
T8192 |
T8193 |
nsubjpass |
use,necessitated |
R2362 |
T8194 |
T8192 |
prep |
of,use |
R2363 |
T8195 |
T8196 |
compound |
tissue,homogenates |
R2364 |
T8196 |
T8194 |
pobj |
homogenates,of |
R2365 |
T8197 |
T8193 |
auxpass |
was,necessitated |
R2366 |
T8198 |
T8193 |
agent |
by,necessitated |
R2367 |
T8199 |
T8200 |
det |
the,availability |
R2368 |
T8200 |
T8198 |
pobj |
availability,by |
R2369 |
T8201 |
T8200 |
amod |
limited,availability |
R2370 |
T8202 |
T8200 |
prep |
of,availability |
R2371 |
T8203 |
T8204 |
compound |
liver,tissue |
R2372 |
T8204 |
T8202 |
pobj |
tissue,of |
R2373 |
T8205 |
T8204 |
punct |
", ",tissue |
R2374 |
T8206 |
T8207 |
advmod |
particularly,from |
R2375 |
T8207 |
T8204 |
prep |
from,tissue |
R2376 |
T8208 |
T8209 |
amod |
newborn,pups |
R2377 |
T8209 |
T8207 |
pobj |
pups,from |
R2378 |
T8210 |
T8193 |
punct |
.,necessitated |
R2379 |
T8212 |
T8213 |
compound |
BCKDH,activity |
R2380 |
T8213 |
T8214 |
nsubjpass |
activity,determined |
R2381 |
T8215 |
T8214 |
auxpass |
was,determined |
R2382 |
T8216 |
T8214 |
prep |
by,determined |
R2383 |
T8217 |
T8216 |
pcomp |
measuring,by |
R2384 |
T8218 |
T8219 |
det |
the,release |
R2385 |
T8219 |
T8217 |
dobj |
release,measuring |
R2386 |
T8220 |
T8219 |
prep |
of,release |
R2387 |
T8221 |
T8220 |
pobj |
14CO2,of |
R2388 |
T8222 |
T8217 |
prep |
from,measuring |
R2389 |
T8223 |
T8224 |
nmod |
α,keto |
R2390 |
T8224 |
T8226 |
nmod |
keto,isocaproate |
R2391 |
T8225 |
T8224 |
punct |
-,keto |
R2392 |
T8226 |
T8222 |
pobj |
isocaproate,from |
R2393 |
T8227 |
T8228 |
punct |
[,1 |
R2394 |
T8228 |
T8224 |
parataxis |
1,keto |
R2395 |
T8229 |
T8230 |
punct |
-,14C |
R2396 |
T8230 |
T8228 |
prep |
14C,1 |
R2397 |
T8231 |
T8228 |
punct |
],1 |
R2398 |
T8232 |
T8233 |
mark |
as,described |
R2399 |
T8233 |
T8214 |
advcl |
described,determined |
R2400 |
T8234 |
T8233 |
advmod |
previously,described |
R2401 |
T8235 |
T8236 |
punct |
[,31 |
R2402 |
T8236 |
T8233 |
parataxis |
31,described |
R2403 |
T8237 |
T8236 |
punct |
],31 |
R2404 |
T8238 |
T8214 |
punct |
.,determined |
R2405 |
T8240 |
T8241 |
det |
The,mixture |
R2406 |
T8241 |
T8244 |
nsubj |
mixture,contained |
R2407 |
T8242 |
T8241 |
amod |
complete,mixture |
R2408 |
T8243 |
T8241 |
compound |
reaction,mixture |
R2409 |
T8245 |
T8246 |
punct |
(,volume |
R2410 |
T8246 |
T8244 |
parataxis |
volume,contained |
R2411 |
T8247 |
T8246 |
amod |
final,volume |
R2412 |
T8248 |
T8249 |
nummod |
1,ml |
R2413 |
T8249 |
T8246 |
appos |
ml,volume |
R2414 |
T8250 |
T8246 |
punct |
),volume |
R2415 |
T8251 |
T8252 |
nummod |
30,mM |
R2416 |
T8252 |
T8253 |
compound |
mM,buffer |
R2417 |
T8253 |
T8244 |
dobj |
buffer,contained |
R2418 |
T8254 |
T8255 |
compound |
potassium,phosphate |
R2419 |
T8255 |
T8253 |
compound |
phosphate,buffer |
R2420 |
T8256 |
T8253 |
punct |
", ",buffer |
R2421 |
T8257 |
T8253 |
npadvmod |
pH,buffer |
R2422 |
T8258 |
T8257 |
nummod |
7.4,pH |
R2423 |
T8259 |
T8253 |
punct |
", ",buffer |
R2424 |
T8260 |
T8261 |
nummod |
0.20,mM |
R2425 |
T8261 |
T8262 |
compound |
mM,ketoisocaproate |
R2426 |
T8262 |
T8253 |
conj |
ketoisocaproate,buffer |
R2427 |
T8263 |
T8262 |
compound |
α,ketoisocaproate |
R2428 |
T8264 |
T8262 |
punct |
-,ketoisocaproate |
R2429 |
T8265 |
T8262 |
punct |
", ",ketoisocaproate |
R2430 |
T8266 |
T8267 |
nummod |
0.40,mM |
R2431 |
T8267 |
T8268 |
compound |
mM,CoASH |
R2432 |
T8268 |
T8262 |
conj |
CoASH,ketoisocaproate |
R2433 |
T8269 |
T8268 |
punct |
", ",CoASH |
R2434 |
T8270 |
T8271 |
nummod |
0.40,mM |
R2435 |
T8271 |
T8272 |
compound |
mM,pyrophosphate |
R2436 |
T8272 |
T8268 |
conj |
pyrophosphate,CoASH |
R2437 |
T8273 |
T8272 |
compound |
thiamin,pyrophosphate |
R2438 |
T8274 |
T8272 |
punct |
", ",pyrophosphate |
R2439 |
T8275 |
T8276 |
nummod |
2,mM |
R2440 |
T8276 |
T8277 |
compound |
mM,NAD |
R2441 |
T8277 |
T8272 |
conj |
NAD,pyrophosphate |
R2442 |
T8278 |
T8277 |
punct |
+,NAD |
R2443 |
T8279 |
T8277 |
punct |
", ",NAD |
R2444 |
T8280 |
T8281 |
nummod |
2,mM |
R2445 |
T8281 |
T8282 |
compound |
mM,dithiothreitol |
R2446 |
T8282 |
T8277 |
conj |
dithiothreitol,NAD |
R2447 |
T8283 |
T8282 |
punct |
", ",dithiothreitol |
R2448 |
T8284 |
T8285 |
nummod |
5,mM |
R2449 |
T8285 |
T8286 |
compound |
mM,Mg2 |
R2450 |
T8286 |
T8282 |
conj |
Mg2,dithiothreitol |
R2451 |
T8287 |
T8286 |
punct |
+,Mg2 |
R2452 |
T8288 |
T8286 |
punct |
", ",Mg2 |
R2453 |
T8289 |
T8290 |
advmod |
approximately,"250,000" |
R2454 |
T8290 |
T8291 |
nummod |
"250,000",DPM |
R2455 |
T8291 |
T8286 |
conj |
DPM,Mg2 |
R2456 |
T8292 |
T8291 |
prep |
of,DPM |
R2457 |
T8293 |
T8294 |
nmod |
α,keto |
R2458 |
T8294 |
T8296 |
nmod |
keto,isocaproate |
R2459 |
T8295 |
T8294 |
punct |
-,keto |
R2460 |
T8296 |
T8292 |
pobj |
isocaproate,of |
R2461 |
T8297 |
T8298 |
punct |
[,1 |
R2462 |
T8298 |
T8294 |
parataxis |
1,keto |
R2463 |
T8299 |
T8300 |
punct |
-,14C |
R2464 |
T8300 |
T8298 |
prep |
14C,1 |
R2465 |
T8301 |
T8298 |
punct |
],1 |
R2466 |
T8302 |
T8291 |
punct |
", ",DPM |
R2467 |
T8303 |
T8291 |
cc |
and,DPM |
R2468 |
T8304 |
T8305 |
nummod |
0.10,ml |
R2469 |
T8305 |
T8291 |
conj |
ml,DPM |
R2470 |
T8306 |
T8305 |
prep |
of,ml |
R2471 |
T8307 |
T8308 |
compound |
liver,homogenate |
R2472 |
T8308 |
T8306 |
pobj |
homogenate,of |
R2473 |
T8309 |
T8310 |
punct |
(,protein |
R2474 |
T8310 |
T8308 |
parataxis |
protein,homogenate |
R2475 |
T8311 |
T8312 |
quantmod |
2,3 |
R2476 |
T8312 |
T8314 |
nummod |
3,mg |
R2477 |
T8313 |
T8312 |
punct |
–,3 |
R2478 |
T8314 |
T8310 |
compound |
mg,protein |
R2479 |
T8315 |
T8310 |
punct |
),protein |
R2480 |
T8316 |
T8244 |
punct |
.,contained |
R2481 |
T8318 |
T8319 |
nsubjpass |
Assays,carried |
R2482 |
T8320 |
T8319 |
auxpass |
were,carried |
R2483 |
T8321 |
T8319 |
prt |
out,carried |
R2484 |
T8322 |
T8319 |
prep |
for,carried |
R2485 |
T8323 |
T8324 |
nummod |
15,min |
R2486 |
T8324 |
T8322 |
pobj |
min,for |
R2487 |
T8325 |
T8319 |
prep |
at,carried |
R2488 |
T8326 |
T8327 |
nummod |
37,°C |
R2489 |
T8327 |
T8325 |
pobj |
°C,at |
R2490 |
T8328 |
T8319 |
punct |
", ",carried |
R2491 |
T8329 |
T8330 |
nsubjpass |
14CO2,trapped |
R2492 |
T8330 |
T8319 |
conj |
trapped,carried |
R2493 |
T8331 |
T8330 |
auxpass |
was,trapped |
R2494 |
T8332 |
T8330 |
prep |
in,trapped |
R2495 |
T8333 |
T8332 |
pobj |
hydroxide,in |
R2496 |
T8334 |
T8333 |
prep |
of,hydroxide |
R2497 |
T8335 |
T8334 |
pobj |
Hyamine,of |
R2498 |
T8336 |
T8330 |
punct |
", ",trapped |
R2499 |
T8337 |
T8330 |
cc |
and,trapped |
R2500 |
T8338 |
T8339 |
nsubjpass |
radioactivity,determined |
R2501 |
T8339 |
T8330 |
conj |
determined,trapped |
R2502 |
T8340 |
T8339 |
auxpass |
was,determined |
R2503 |
T8341 |
T8339 |
prep |
by,determined |
R2504 |
T8342 |
T8343 |
amod |
liquid,scintillation |
R2505 |
T8343 |
T8344 |
compound |
scintillation,spectrometry |
R2506 |
T8344 |
T8341 |
pobj |
spectrometry,by |
R2507 |
T8345 |
T8339 |
punct |
.,determined |