PMC:1448208 / 10268-11493
Annnotations
craft-sa-dev
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T5872 | 0-10 | NN | denotes | Production |
T5873 | 11-13 | IN | denotes | of |
T5874 | 14-24 | JJ | denotes | transgenic |
T5875 | 25-29 | NNS | denotes | mice |
T5876 | 29-116 | sentence | denotes | Standard molecular techniques were used to assemble the transgenic construct, pTRE-E2. |
T5877 | 30-38 | JJ | denotes | Standard |
T5878 | 49-59 | NNS | denotes | techniques |
T5879 | 39-48 | JJ | denotes | molecular |
T5880 | 65-69 | VBN | denotes | used |
T5881 | 60-64 | VBD | denotes | were |
T5882 | 70-72 | TO | denotes | to |
T5883 | 73-81 | VB | denotes | assemble |
T5884 | 82-85 | DT | denotes | the |
T5885 | 97-106 | NN | denotes | construct |
T5886 | 86-96 | JJ | denotes | transgenic |
T5887 | 106-108 | , | denotes | , |
T5888 | 108-112 | NN | denotes | pTRE |
T5889 | 113-115 | NN | denotes | E2 |
T5890 | 112-113 | HYPH | denotes | - |
T5891 | 115-116 | . | denotes | . |
T5892 | 116-740 | sentence | denotes | This transgene contains the tetracycline responsive hCMV*-1 promoter [consisting of the tetracycline responsive element (TRE) and a minimal hCMV promoter] [25] from pTRE2 (Clontech Inc., Mt. View, CA), a chimeric intron from pCI (Promega) to increase message stability and expression [26,27], a Kozak consensus sequence at the initiation codon to optimize translation [28], the human E2 cDNA which has been modified to contain a 4× alanine linker followed by a c-myc epitope tag at the carboxy terminus to facilitate detection, and an SV40 late polyadenylation sequence from pCI for enhanced mRNA stability and translation. |
T5893 | 117-121 | DT | denotes | This |
T5894 | 122-131 | NN | denotes | transgene |
T5895 | 132-140 | VBZ | denotes | contains |
T5896 | 141-144 | DT | denotes | the |
T5897 | 177-185 | NN | denotes | promoter |
T5898 | 145-157 | NN | denotes | tetracycline |
T5899 | 158-168 | JJ | denotes | responsive |
T5900 | 169-173 | NN | denotes | hCMV |
T5901 | 173-174 | SYM | denotes | * |
T5902 | 174-175 | HYPH | denotes | - |
T5903 | 175-176 | CD | denotes | 1 |
T5904 | 186-187 | -LRB- | denotes | [ |
T5905 | 187-197 | VBG | denotes | consisting |
T5906 | 198-200 | IN | denotes | of |
T5907 | 201-204 | DT | denotes | the |
T5908 | 229-236 | NN | denotes | element |
T5909 | 205-217 | NN | denotes | tetracycline |
T5910 | 218-228 | JJ | denotes | responsive |
T5911 | 237-238 | -LRB- | denotes | ( |
T5912 | 238-241 | NN | denotes | TRE |
T5913 | 241-242 | -RRB- | denotes | ) |
T5914 | 243-246 | CC | denotes | and |
T5915 | 247-248 | DT | denotes | a |
T5916 | 262-270 | NN | denotes | promoter |
T5917 | 249-256 | JJ | denotes | minimal |
T5918 | 257-261 | NN | denotes | hCMV |
T5919 | 270-271 | -RRB- | denotes | ] |
T5920 | 272-273 | -LRB- | denotes | [ |
T5921 | 273-275 | CD | denotes | 25 |
T5922 | 275-276 | -RRB- | denotes | ] |
T5923 | 277-281 | IN | denotes | from |
T5924 | 282-287 | NN | denotes | pTRE2 |
T5925 | 288-289 | -LRB- | denotes | ( |
T5926 | 298-302 | NNP | denotes | Inc. |
T5927 | 289-297 | NNP | denotes | Clontech |
T5928 | 302-304 | , | denotes | , |
T5929 | 304-307 | NNP | denotes | Mt. |
T5930 | 308-312 | NNP | denotes | View |
T5931 | 312-314 | , | denotes | , |
T5932 | 314-316 | NNP | denotes | CA |
T5933 | 316-317 | -RRB- | denotes | ) |
T5934 | 317-319 | , | denotes | , |
T5935 | 319-320 | DT | denotes | a |
T5936 | 330-336 | NN | denotes | intron |
T5937 | 321-329 | JJ | denotes | chimeric |
T5938 | 337-341 | IN | denotes | from |
T5939 | 342-345 | NN | denotes | pCI |
T5940 | 346-347 | -LRB- | denotes | ( |
T5941 | 347-354 | NNP | denotes | Promega |
T5942 | 354-355 | -RRB- | denotes | ) |
T5943 | 356-358 | TO | denotes | to |
T5944 | 359-367 | VB | denotes | increase |
T5945 | 368-375 | NN | denotes | message |
T5946 | 376-385 | NN | denotes | stability |
T5947 | 386-389 | CC | denotes | and |
T5948 | 390-400 | NN | denotes | expression |
T5949 | 401-402 | -LRB- | denotes | [ |
T5950 | 405-407 | CD | denotes | 27 |
T5951 | 402-404 | CD | denotes | 26 |
T5952 | 404-405 | , | denotes | , |
T5953 | 407-408 | -RRB- | denotes | ] |
T5954 | 408-410 | , | denotes | , |
T5955 | 410-411 | DT | denotes | a |
T5956 | 428-436 | NN | denotes | sequence |
T5957 | 412-417 | NN | denotes | Kozak |
T5958 | 418-427 | NN | denotes | consensus |
T5959 | 437-439 | IN | denotes | at |
T5960 | 440-443 | DT | denotes | the |
T5961 | 455-460 | NN | denotes | codon |
T5962 | 444-454 | NN | denotes | initiation |
T5963 | 461-463 | TO | denotes | to |
T5964 | 464-472 | VB | denotes | optimize |
T5965 | 473-484 | NN | denotes | translation |
T5966 | 485-486 | -LRB- | denotes | [ |
T5967 | 486-488 | CD | denotes | 28 |
T5968 | 488-489 | -RRB- | denotes | ] |
T5969 | 489-491 | , | denotes | , |
T5970 | 491-494 | DT | denotes | the |
T5971 | 504-508 | NN | denotes | cDNA |
T5972 | 495-500 | JJ | denotes | human |
T5973 | 501-503 | NN | denotes | E2 |
T5974 | 509-514 | WDT | denotes | which |
T5975 | 524-532 | VBN | denotes | modified |
T5976 | 515-518 | VBZ | denotes | has |
T5977 | 519-523 | VBN | denotes | been |
T5978 | 533-535 | TO | denotes | to |
T5979 | 536-543 | VB | denotes | contain |
T5980 | 544-545 | DT | denotes | a |
T5981 | 557-563 | NN | denotes | linker |
T5982 | 546-547 | CD | denotes | 4 |
T5983 | 547-548 | SYM | denotes | × |
T5984 | 549-556 | NN | denotes | alanine |
T5985 | 564-572 | VBN | denotes | followed |
T5986 | 573-575 | IN | denotes | by |
T5987 | 576-577 | DT | denotes | a |
T5988 | 592-595 | NN | denotes | tag |
T5989 | 578-579 | NN | denotes | c |
T5990 | 580-583 | NN | denotes | myc |
T5991 | 579-580 | HYPH | denotes | - |
T5992 | 584-591 | NN | denotes | epitope |
T5993 | 596-598 | IN | denotes | at |
T5994 | 599-602 | DT | denotes | the |
T5995 | 611-619 | NN | denotes | terminus |
T5996 | 603-610 | NN | denotes | carboxy |
T5997 | 620-622 | TO | denotes | to |
T5998 | 623-633 | VB | denotes | facilitate |
T5999 | 634-643 | NN | denotes | detection |
T6000 | 643-645 | , | denotes | , |
T6001 | 645-648 | CC | denotes | and |
T6002 | 649-651 | DT | denotes | an |
T6003 | 678-686 | NN | denotes | sequence |
T6004 | 652-656 | NN | denotes | SV40 |
T6005 | 657-661 | JJ | denotes | late |
T6006 | 662-677 | NN | denotes | polyadenylation |
T6007 | 687-691 | IN | denotes | from |
T6008 | 692-695 | NN | denotes | pCI |
T6009 | 696-699 | IN | denotes | for |
T6010 | 700-708 | VBN | denotes | enhanced |
T6011 | 714-723 | NN | denotes | stability |
T6012 | 709-713 | NN | denotes | mRNA |
T6013 | 724-727 | CC | denotes | and |
T6014 | 728-739 | NN | denotes | translation |
T6015 | 739-740 | . | denotes | . |
T6016 | 740-1028 | sentence | denotes | The 2.48 kb TRE-E2 transgene was purified from vector sequences following digestion with XhoI and BamHI and injected into C57BL/6J or C57BL/6J × Strain 129SvEv mouse embryos at the transgenic core facilities of the University of Pittsburgh and the University of Cincinnati, respectively. |
T6017 | 741-744 | DT | denotes | The |
T6018 | 760-769 | NN | denotes | transgene |
T6019 | 745-749 | CD | denotes | 2.48 |
T6020 | 750-752 | NN | denotes | kb |
T6021 | 753-756 | NN | denotes | TRE |
T6022 | 757-759 | NN | denotes | E2 |
T6023 | 756-757 | HYPH | denotes | - |
T6024 | 774-782 | VBN | denotes | purified |
T6025 | 770-773 | VBD | denotes | was |
T6026 | 783-787 | IN | denotes | from |
T6027 | 788-794 | NN | denotes | vector |
T6028 | 795-804 | NNS | denotes | sequences |
T6029 | 805-814 | VBG | denotes | following |
T6030 | 815-824 | NN | denotes | digestion |
T6031 | 825-829 | IN | denotes | with |
T6032 | 830-834 | NN | denotes | XhoI |
T6033 | 835-838 | CC | denotes | and |
T6034 | 839-844 | NN | denotes | BamHI |
T6035 | 845-848 | CC | denotes | and |
T6036 | 849-857 | VBN | denotes | injected |
T6037 | 858-862 | IN | denotes | into |
T6038 | 863-868 | NN | denotes | C57BL |
T6039 | 869-871 | NN | denotes | 6J |
T6040 | 868-869 | HYPH | denotes | / |
T6041 | 886-892 | NN | denotes | Strain |
T6042 | 872-874 | CC | denotes | or |
T6043 | 875-880 | NN | denotes | C57BL |
T6044 | 881-883 | NN | denotes | 6J |
T6045 | 880-881 | HYPH | denotes | / |
T6046 | 884-885 | SYM | denotes | × |
T6047 | 907-914 | NNS | denotes | embryos |
T6048 | 893-900 | NN | denotes | 129SvEv |
T6049 | 901-906 | NN | denotes | mouse |
T6050 | 915-917 | IN | denotes | at |
T6051 | 918-921 | DT | denotes | the |
T6052 | 938-948 | NNS | denotes | facilities |
T6053 | 922-932 | JJ | denotes | transgenic |
T6054 | 933-937 | NN | denotes | core |
T6055 | 949-951 | IN | denotes | of |
T6056 | 952-955 | DT | denotes | the |
T6057 | 956-966 | NNP | denotes | University |
T6058 | 967-969 | IN | denotes | of |
T6059 | 970-980 | NNP | denotes | Pittsburgh |
T6060 | 981-984 | CC | denotes | and |
T6061 | 985-988 | DT | denotes | the |
T6062 | 989-999 | NNP | denotes | University |
T6063 | 1000-1002 | IN | denotes | of |
T6064 | 1003-1013 | NNP | denotes | Cincinnati |
T6065 | 1013-1015 | , | denotes | , |
T6066 | 1015-1027 | RB | denotes | respectively |
T6067 | 1027-1028 | . | denotes | . |
T6068 | 1028-1225 | sentence | denotes | Genomic DNA from the tail of mice was screened by Southern blot analysis following digestion with EcoRI and hybridization to an ~400 bp probe derived from the SV40 portion of the TRE-E2 transgene. |
T6069 | 1029-1036 | JJ | denotes | Genomic |
T6070 | 1037-1040 | NN | denotes | DNA |
T6071 | 1067-1075 | VBN | denotes | screened |
T6072 | 1041-1045 | IN | denotes | from |
T6073 | 1046-1049 | DT | denotes | the |
T6074 | 1050-1054 | NN | denotes | tail |
T6075 | 1055-1057 | IN | denotes | of |
T6076 | 1058-1062 | NNS | denotes | mice |
T6077 | 1063-1066 | VBD | denotes | was |
T6078 | 1076-1078 | IN | denotes | by |
T6079 | 1079-1087 | NNP | denotes | Southern |
T6080 | 1088-1092 | NN | denotes | blot |
T6081 | 1093-1101 | NN | denotes | analysis |
T6082 | 1102-1111 | VBG | denotes | following |
T6083 | 1112-1121 | NN | denotes | digestion |
T6084 | 1122-1126 | IN | denotes | with |
T6085 | 1127-1132 | NN | denotes | EcoRI |
T6086 | 1133-1136 | CC | denotes | and |
T6087 | 1137-1150 | NN | denotes | hybridization |
T6088 | 1151-1153 | IN | denotes | to |
T6089 | 1154-1156 | DT | denotes | an |
T6090 | 1165-1170 | NN | denotes | probe |
T6091 | 1157-1158 | SYM | denotes | ~ |
T6092 | 1158-1161 | CD | denotes | 400 |
T6093 | 1162-1164 | NN | denotes | bp |
T6094 | 1171-1178 | VBN | denotes | derived |
T6095 | 1179-1183 | IN | denotes | from |
T6096 | 1184-1187 | DT | denotes | the |
T6097 | 1193-1200 | NN | denotes | portion |
T6098 | 1188-1192 | NN | denotes | SV40 |
T6099 | 1201-1203 | IN | denotes | of |
T6100 | 1204-1207 | DT | denotes | the |
T6101 | 1215-1224 | NN | denotes | transgene |
T6102 | 1208-1211 | NN | denotes | TRE |
T6103 | 1212-1214 | NN | denotes | E2 |
T6104 | 1211-1212 | HYPH | denotes | - |
T6105 | 1224-1225 | . | denotes | . |
R1603 | T5873 | T5872 | prep | of,Production |
R1604 | T5874 | T5875 | amod | transgenic,mice |
R1605 | T5875 | T5873 | pobj | mice,of |
R1606 | T5877 | T5878 | amod | Standard,techniques |
R1607 | T5878 | T5880 | nsubjpass | techniques,used |
R1608 | T5879 | T5878 | amod | molecular,techniques |
R1609 | T5881 | T5880 | auxpass | were,used |
R1610 | T5882 | T5883 | aux | to,assemble |
R1611 | T5883 | T5880 | advcl | assemble,used |
R1612 | T5884 | T5885 | det | the,construct |
R1613 | T5885 | T5883 | dobj | construct,assemble |
R1614 | T5886 | T5885 | amod | transgenic,construct |
R1615 | T5887 | T5885 | punct | ", ",construct |
R1616 | T5888 | T5889 | compound | pTRE,E2 |
R1617 | T5889 | T5885 | appos | E2,construct |
R1618 | T5890 | T5889 | punct | -,E2 |
R1619 | T5891 | T5880 | punct | .,used |
R1620 | T5893 | T5894 | det | This,transgene |
R1621 | T5894 | T5895 | nsubj | transgene,contains |
R1622 | T5896 | T5897 | det | the,promoter |
R1623 | T5897 | T5895 | dobj | promoter,contains |
R1624 | T5898 | T5897 | nmod | tetracycline,promoter |
R1625 | T5899 | T5897 | amod | responsive,promoter |
R1626 | T5900 | T5897 | nmod | hCMV,promoter |
R1627 | T5901 | T5900 | punct | *,hCMV |
R1628 | T5902 | T5900 | punct | -,hCMV |
R1629 | T5903 | T5900 | nummod | 1,hCMV |
R1630 | T5904 | T5897 | punct | [,promoter |
R1631 | T5905 | T5897 | acl | consisting,promoter |
R1632 | T5906 | T5905 | prep | of,consisting |
R1633 | T5907 | T5908 | det | the,element |
R1634 | T5908 | T5906 | pobj | element,of |
R1635 | T5909 | T5908 | nmod | tetracycline,element |
R1636 | T5910 | T5908 | amod | responsive,element |
R1637 | T5911 | T5908 | punct | (,element |
R1638 | T5912 | T5908 | appos | TRE,element |
R1639 | T5913 | T5908 | punct | ),element |
R1640 | T5914 | T5908 | cc | and,element |
R1641 | T5915 | T5916 | det | a,promoter |
R1642 | T5916 | T5908 | conj | promoter,element |
R1643 | T5917 | T5916 | amod | minimal,promoter |
R1644 | T5918 | T5916 | compound | hCMV,promoter |
R1645 | T5919 | T5916 | punct | ],promoter |
R1646 | T5920 | T5921 | punct | [,25 |
R1647 | T5921 | T5916 | parataxis | 25,promoter |
R1648 | T5922 | T5921 | punct | ],25 |
R1649 | T5923 | T5916 | prep | from,promoter |
R1650 | T5924 | T5923 | pobj | pTRE2,from |
R1651 | T5925 | T5926 | punct | (,Inc. |
R1652 | T5926 | T5916 | parataxis | Inc.,promoter |
R1653 | T5927 | T5926 | compound | Clontech,Inc. |
R1654 | T5928 | T5926 | punct | ", ",Inc. |
R1655 | T5929 | T5930 | compound | Mt.,View |
R1656 | T5930 | T5926 | npadvmod | View,Inc. |
R1657 | T5931 | T5926 | punct | ", ",Inc. |
R1658 | T5932 | T5926 | npadvmod | CA,Inc. |
R1659 | T5933 | T5926 | punct | ),Inc. |
R1660 | T5934 | T5897 | punct | ", ",promoter |
R1661 | T5935 | T5936 | det | a,intron |
R1662 | T5936 | T5897 | conj | intron,promoter |
R1663 | T5937 | T5936 | amod | chimeric,intron |
R1664 | T5938 | T5936 | prep | from,intron |
R1665 | T5939 | T5938 | pobj | pCI,from |
R1666 | T5940 | T5941 | punct | (,Promega |
R1667 | T5941 | T5936 | parataxis | Promega,intron |
R1668 | T5942 | T5941 | punct | ),Promega |
R1669 | T5943 | T5944 | aux | to,increase |
R1670 | T5944 | T5936 | advcl | increase,intron |
R1671 | T5945 | T5946 | compound | message,stability |
R1672 | T5946 | T5944 | dobj | stability,increase |
R1673 | T5947 | T5946 | cc | and,stability |
R1674 | T5948 | T5946 | conj | expression,stability |
R1675 | T5949 | T5950 | punct | [,27 |
R1676 | T5950 | T5944 | parataxis | 27,increase |
R1677 | T5951 | T5950 | nummod | 26,27 |
R1678 | T5952 | T5950 | punct | ",",27 |
R1679 | T5953 | T5950 | punct | ],27 |
R1680 | T5954 | T5936 | punct | ", ",intron |
R1681 | T5955 | T5956 | det | a,sequence |
R1682 | T5956 | T5936 | conj | sequence,intron |
R1683 | T5957 | T5956 | compound | Kozak,sequence |
R1684 | T5958 | T5956 | compound | consensus,sequence |
R1685 | T5959 | T5956 | prep | at,sequence |
R1686 | T5960 | T5961 | det | the,codon |
R1687 | T5961 | T5959 | pobj | codon,at |
R1688 | T5962 | T5961 | compound | initiation,codon |
R1689 | T5963 | T5964 | aux | to,optimize |
R1690 | T5964 | T5956 | advcl | optimize,sequence |
R1691 | T5965 | T5964 | dobj | translation,optimize |
R1692 | T5966 | T5967 | punct | [,28 |
R1693 | T5967 | T5964 | parataxis | 28,optimize |
R1694 | T5968 | T5967 | punct | ],28 |
R1695 | T5969 | T5956 | punct | ", ",sequence |
R1696 | T5970 | T5971 | det | the,cDNA |
R1697 | T5971 | T5956 | conj | cDNA,sequence |
R1698 | T5972 | T5971 | amod | human,cDNA |
R1699 | T5973 | T5971 | compound | E2,cDNA |
R1700 | T5974 | T5975 | dep | which,modified |
R1701 | T5975 | T5971 | relcl | modified,cDNA |
R1702 | T5976 | T5975 | aux | has,modified |
R1703 | T5977 | T5975 | auxpass | been,modified |
R1704 | T5978 | T5979 | aux | to,contain |
R1705 | T5979 | T5975 | advcl | contain,modified |
R1706 | T5980 | T5981 | det | a,linker |
R1707 | T5981 | T5979 | dobj | linker,contain |
R1708 | T5982 | T5981 | nummod | 4,linker |
R1709 | T5983 | T5982 | punct | ×,4 |
R1710 | T5984 | T5981 | compound | alanine,linker |
R1711 | T5985 | T5981 | acl | followed,linker |
R1712 | T5986 | T5985 | agent | by,followed |
R1713 | T5987 | T5988 | det | a,tag |
R1714 | T5988 | T5986 | pobj | tag,by |
R1715 | T5989 | T5990 | compound | c,myc |
R1716 | T5990 | T5988 | compound | myc,tag |
R1717 | T5991 | T5990 | punct | -,myc |
R1718 | T5992 | T5988 | compound | epitope,tag |
R1719 | T5993 | T5988 | prep | at,tag |
R1720 | T5994 | T5995 | det | the,terminus |
R1721 | T5995 | T5993 | pobj | terminus,at |
R1722 | T5996 | T5995 | compound | carboxy,terminus |
R1723 | T5997 | T5998 | aux | to,facilitate |
R1724 | T5998 | T5988 | advcl | facilitate,tag |
R1725 | T5999 | T5998 | dobj | detection,facilitate |
R1726 | T6000 | T5971 | punct | ", ",cDNA |
R1727 | T6001 | T5971 | cc | and,cDNA |
R1728 | T6002 | T6003 | det | an,sequence |
R1729 | T6003 | T5971 | conj | sequence,cDNA |
R1730 | T6004 | T6003 | nmod | SV40,sequence |
R1731 | T6005 | T6003 | amod | late,sequence |
R1732 | T6006 | T6003 | compound | polyadenylation,sequence |
R1733 | T6007 | T6003 | prep | from,sequence |
R1734 | T6008 | T6007 | pobj | pCI,from |
R1735 | T6009 | T6003 | prep | for,sequence |
R1736 | T6010 | T6011 | amod | enhanced,stability |
R1737 | T6011 | T6009 | pobj | stability,for |
R1738 | T6012 | T6011 | compound | mRNA,stability |
R1739 | T6013 | T6011 | cc | and,stability |
R1740 | T6014 | T6011 | conj | translation,stability |
R1741 | T6015 | T5895 | punct | .,contains |
R1742 | T6017 | T6018 | det | The,transgene |
R1743 | T6018 | T6024 | nsubj | transgene,purified |
R1744 | T6019 | T6020 | nummod | 2.48,kb |
R1745 | T6020 | T6018 | compound | kb,transgene |
R1746 | T6021 | T6022 | compound | TRE,E2 |
R1747 | T6022 | T6018 | compound | E2,transgene |
R1748 | T6023 | T6022 | punct | -,E2 |
R1749 | T6025 | T6024 | aux | was,purified |
R1750 | T6026 | T6024 | prep | from,purified |
R1751 | T6027 | T6028 | compound | vector,sequences |
R1752 | T6028 | T6026 | pobj | sequences,from |
R1753 | T6029 | T6024 | prep | following,purified |
R1754 | T6030 | T6029 | pobj | digestion,following |
R1755 | T6031 | T6030 | prep | with,digestion |
R1756 | T6032 | T6031 | pobj | XhoI,with |
R1757 | T6033 | T6032 | cc | and,XhoI |
R1758 | T6034 | T6032 | conj | BamHI,XhoI |
R1759 | T6035 | T6024 | cc | and,purified |
R1760 | T6036 | T6024 | conj | injected,purified |
R1761 | T6037 | T6036 | prep | into,injected |
R1762 | T6038 | T6039 | nmod | C57BL,6J |
R1763 | T6039 | T6041 | nmod | 6J,Strain |
R1764 | T6040 | T6039 | punct | /,6J |
R1765 | T6041 | T6047 | compound | Strain,embryos |
R1766 | T6042 | T6039 | cc | or,6J |
R1767 | T6043 | T6044 | compound | C57BL,6J |
R1768 | T6044 | T6039 | conj | 6J,6J |
R1769 | T6045 | T6044 | punct | /,6J |
R1770 | T6046 | T6041 | punct | ×,Strain |
R1771 | T6047 | T6037 | pobj | embryos,into |
R1772 | T6048 | T6047 | compound | 129SvEv,embryos |
R1773 | T6049 | T6047 | compound | mouse,embryos |
R1774 | T6050 | T6024 | prep | at,purified |
R1775 | T6051 | T6052 | det | the,facilities |
R1776 | T6052 | T6050 | pobj | facilities,at |
R1777 | T6053 | T6052 | amod | transgenic,facilities |
R1778 | T6054 | T6052 | compound | core,facilities |
R1779 | T6055 | T6052 | prep | of,facilities |
R1780 | T6056 | T6057 | det | the,University |
R1781 | T6057 | T6055 | pobj | University,of |
R1782 | T6058 | T6057 | prep | of,University |
R1783 | T6059 | T6058 | pobj | Pittsburgh,of |
R1784 | T6060 | T6057 | cc | and,University |
R1785 | T6061 | T6062 | det | the,University |
R1786 | T6062 | T6057 | conj | University,University |
R1787 | T6063 | T6062 | prep | of,University |
R1788 | T6064 | T6063 | pobj | Cincinnati,of |
R1789 | T6065 | T6024 | punct | ", ",purified |
R1790 | T6066 | T6024 | advmod | respectively,purified |
R1791 | T6067 | T6024 | punct | .,purified |
R1792 | T6069 | T6070 | amod | Genomic,DNA |
R1793 | T6070 | T6071 | nsubjpass | DNA,screened |
R1794 | T6072 | T6070 | prep | from,DNA |
R1795 | T6073 | T6074 | det | the,tail |
R1796 | T6074 | T6072 | pobj | tail,from |
R1797 | T6075 | T6074 | prep | of,tail |
R1798 | T6076 | T6075 | pobj | mice,of |
R1799 | T6077 | T6071 | auxpass | was,screened |
R1800 | T6078 | T6071 | prep | by,screened |
R1801 | T6079 | T6080 | compound | Southern,blot |
R1802 | T6080 | T6081 | compound | blot,analysis |
R1803 | T6081 | T6078 | pobj | analysis,by |
R1804 | T6082 | T6071 | prep | following,screened |
R1805 | T6083 | T6082 | pobj | digestion,following |
R1806 | T6084 | T6083 | prep | with,digestion |
R1807 | T6085 | T6084 | pobj | EcoRI,with |
R1808 | T6086 | T6083 | cc | and,digestion |
R1809 | T6087 | T6083 | conj | hybridization,digestion |
R1810 | T6088 | T6087 | prep | to,hybridization |
R1811 | T6089 | T6090 | det | an,probe |
R1812 | T6090 | T6088 | pobj | probe,to |
R1813 | T6091 | T6092 | punct | ~,400 |
R1814 | T6092 | T6093 | nummod | 400,bp |
R1815 | T6093 | T6090 | compound | bp,probe |
R1816 | T6094 | T6090 | acl | derived,probe |
R1817 | T6095 | T6094 | prep | from,derived |
R1818 | T6096 | T6097 | det | the,portion |
R1819 | T6097 | T6095 | pobj | portion,from |
R1820 | T6098 | T6097 | compound | SV40,portion |
R1821 | T6099 | T6097 | prep | of,portion |
R1822 | T6100 | T6101 | det | the,transgene |
R1823 | T6101 | T6099 | pobj | transgene,of |
R1824 | T6102 | T6103 | compound | TRE,E2 |
R1825 | T6103 | T6101 | compound | E2,transgene |
R1826 | T6104 | T6103 | punct | -,E2 |
R1827 | T6105 | T6071 | punct | .,screened |
craft-ca-core-ex-dev
Below, discontinuous spans are shown in the chain model. You can change it to the bag model.
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T5818 | 14-24 | SO_EXT:transgenic_entity | denotes | transgenic |
T5819 | 25-29 | NCBITaxon:10088 | denotes | mice |
T5820 | 39-48 | CHEBI_EXT:polyatomic_entity_or_group | denotes | molecular |
T5821 | 86-96 | SO_EXT:transgenic_entity | denotes | transgenic |
T5822 | 97-106 | SO_EXT:engineered_biological_sequence | denotes | construct |
T5823 | 113-115 | PR_EXT:000006300 | denotes | E2 |
T5824 | 122-131 | SO_EXT:0000902 | denotes | transgene |
T5825 | 145-157 | CHEBI:27902 | denotes | tetracycline |
T5826 | 169-173 | NCBITaxon:10359 | denotes | hCMV |
T5827 | 177-185 | SO_EXT:0000167 | denotes | promoter |
T5828 | 205-217 | CHEBI:27902 | denotes | tetracycline |
T5829 | 257-261 | NCBITaxon:10359 | denotes | hCMV |
T5830 | 262-270 | SO_EXT:0000167 | denotes | promoter |
T5831 | 330-336 | SO_EXT:0000188 | denotes | intron |
T5832 | 368-375 | SO_EXT:0000673 | denotes | message |
T5833 | 368-375 | CHEBI:33695 | denotes | message |
T5834 | 390-400 | GO:0010467 | denotes | expression |
T5835 | 418-427 | SO:0000993 | denotes | consensus |
T5836 | 428-436 | SO_EXT:biological_sequence | denotes | sequence |
T5837 | 444-460 | SO_EXT:0000318 | denotes | initiation codon |
T5838 | 473-484 | GO:0006412 | denotes | translation |
T5839 | 495-500 | NCBITaxon:9606 | denotes | human |
T5840 | 501-503 | PR_EXT:000006300 | denotes | E2 |
T5841 | 504-508 | SO_EXT:cDNA | denotes | cDNA |
T5842 | 524-532 | SO_EXT:sequence_alteration_process | denotes | modified |
T5843 | 549-556 | CHEBI_SO_EXT:alanine | denotes | alanine |
T5844 | 578-583 | PR_EXT:000000084 | denotes | c-myc |
T5845 | 584-591 | CHEBI_SO_EXT:epitope | denotes | epitope |
T5846 | 592-595 | CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag | denotes | tag |
T5847 | 603-619 | CHEBI_SO_EXT:C_terminus_or_C_terminal_region | denotes | carboxy terminus |
T5848 | 652-656 | NCBITaxon:10633 | denotes | SV40 |
T5849 | 662-677 | GO:0043631 | denotes | polyadenylation |
T5850 | 662-686 | SO_EXT:0000610 | denotes | polyadenylation sequence |
T5851 | 709-713 | CHEBI_SO_EXT:mRNA | denotes | mRNA |
T5852 | 728-739 | GO:0006412 | denotes | translation |
T5853 | 751-752 | CHEBI_SO_EXT:base | denotes | b |
T5854 | 757-759 | PR_EXT:000006300 | denotes | E2 |
T5855 | 760-769 | SO_EXT:0000902 | denotes | transgene |
T5856 | 788-794 | SO_EXT:0000440 | denotes | vector |
T5857 | 795-804 | SO_EXT:biological_sequence | denotes | sequences |
T5858 | 839-844 | PR_EXT:P23940 | denotes | BamHI |
T5859 | 901-906 | NCBITaxon:10088 | denotes | mouse |
T5860 | 907-914 | UBERON:0000922 | denotes | embryos |
T5861 | 922-932 | SO_EXT:transgenic_entity | denotes | transgenic |
T5862 | 1029-1040 | SO_EXT:genomic_DNA | denotes | Genomic DNA |
T5863 | 1037-1040 | CHEBI_SO_EXT:DNA | denotes | DNA |
T5864 | 1050-1054 | UBERON:0002415 | denotes | tail |
T5865 | 1058-1062 | NCBITaxon:10088 | denotes | mice |
T5866 | 1137-1150 | GO:0097617 | denotes | hybridization |
T5867 | 1162-1164 | SO_EXT:0000028 | denotes | bp |
T5868 | 1165-1170 | CHEBI_SO_EXT:molecular_probe | denotes | probe |
T5869 | 1188-1192 | NCBITaxon:10633 | denotes | SV40 |
T5870 | 1212-1214 | PR_EXT:000006300 | denotes | E2 |
T5871 | 1215-1224 | SO_EXT:0000902 | denotes | transgene |
craft-ca-core-dev
Below, discontinuous spans are shown in the chain model. You can change it to the bag model.
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T5781 | 25-29 | NCBITaxon:10088 | denotes | mice |
T5782 | 113-115 | PR:000006300 | denotes | E2 |
T5783 | 122-131 | SO:0000902 | denotes | transgene |
T5784 | 145-157 | CHEBI:27902 | denotes | tetracycline |
T5785 | 169-173 | NCBITaxon:10359 | denotes | hCMV |
T5786 | 177-185 | SO:0000167 | denotes | promoter |
T5787 | 205-217 | CHEBI:27902 | denotes | tetracycline |
T5788 | 257-261 | NCBITaxon:10359 | denotes | hCMV |
T5789 | 262-270 | SO:0000167 | denotes | promoter |
T5790 | 330-336 | SO:0000188 | denotes | intron |
T5791 | 368-375 | SO:0000673 | denotes | message |
T5792 | 368-375 | CHEBI:33695 | denotes | message |
T5793 | 390-400 | GO:0010467 | denotes | expression |
T5794 | 418-427 | SO:0000993 | denotes | consensus |
T5795 | 444-460 | SO:0000318 | denotes | initiation codon |
T5796 | 473-484 | GO:0006412 | denotes | translation |
T5797 | 495-500 | NCBITaxon:9606 | denotes | human |
T5798 | 501-503 | PR:000006300 | denotes | E2 |
T5799 | 578-583 | PR:000000084 | denotes | c-myc |
T5800 | 652-656 | NCBITaxon:10633 | denotes | SV40 |
T5801 | 662-677 | GO:0043631 | denotes | polyadenylation |
T5802 | 662-686 | SO:0000610 | denotes | polyadenylation sequence |
T5803 | 728-739 | GO:0006412 | denotes | translation |
T5804 | 757-759 | PR:000006300 | denotes | E2 |
T5805 | 760-769 | SO:0000902 | denotes | transgene |
T5806 | 788-794 | SO:0000440 | denotes | vector |
T5807 | 839-844 | PR:P23940 | denotes | BamHI |
T5808 | 901-906 | NCBITaxon:10088 | denotes | mouse |
T5809 | 907-914 | UBERON:0000922 | denotes | embryos |
T5810 | 1029-1036 | SO:0001026 | denotes | Genomic |
T5811 | 1050-1054 | UBERON:0002415 | denotes | tail |
T5812 | 1058-1062 | NCBITaxon:10088 | denotes | mice |
T5813 | 1137-1150 | GO:0097617 | denotes | hybridization |
T5814 | 1162-1164 | SO:0000028 | denotes | bp |
T5815 | 1188-1192 | NCBITaxon:10633 | denotes | SV40 |
T5816 | 1212-1214 | PR:000006300 | denotes | E2 |
T5817 | 1215-1224 | SO:0000902 | denotes | transgene |