PMC:1447668 / 9290-10544 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/1447668","sourcedb":"PMC","sourceid":"1447668","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1447668","text":"Figure 1 Foxp3 Suppresses NF-κB-Dependent Transcription\n(A and B) HEK 293T cells (5 × 105) were seeded into six-well plates 1 d prior to transfection with an NF-κB luciferase reporter vector (200 ng) in the presence or absence of indicated concentrations of a Foxp3 expression vector or a control vector and internal reference plasmid (pGL4-TKhRluc2; 50 ng). Cells were harvested 24 h posttransfection and analyzed for luciferase activity (A) and Foxp3 mRNA expression (B).\n(C) HEK 293T cells (5 × 105) were seeded into six-well plates 1 d prior to transfection with an NF-κB luciferase reporter vector (200 ng) in the presence or absence of a Foxp3 expression vector or a control vector (1,500 ng) and internal reference plasmid (pGL4-TKhRluc2; 50 ng). Cells were harvested at 24, 48, 72, and 96 h posttransfection and analyzed for luciferase activity.\n(D) CD4+ T cells (2 × 106) from three healthy donors were nucleofected with an NF-κB luciferase reporter vector (1,000 ng) and Foxp3 or control expression vectors (2,000 ng) and internal reference plasmid (1,000 ng). Cells were harvested 24 h posttransfection and analyzed for luciferase activity. Relative luciferase activity shown in A, C, and D was normalized to the internal reference control.\n\n","divisions":[{"label":"Title","span":{"begin":10,"end":56}}],"tracks":[{"project":"sentences","denotations":[{"id":"T46","span":{"begin":0,"end":56},"obj":"Sentence"},{"id":"T47","span":{"begin":57,"end":359},"obj":"Sentence"},{"id":"T48","span":{"begin":360,"end":474},"obj":"Sentence"},{"id":"T49","span":{"begin":475,"end":754},"obj":"Sentence"},{"id":"T50","span":{"begin":755,"end":854},"obj":"Sentence"},{"id":"T51","span":{"begin":855,"end":1071},"obj":"Sentence"},{"id":"T52","span":{"begin":1072,"end":1152},"obj":"Sentence"},{"id":"T53","span":{"begin":1153,"end":1252},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T46","pred":"source","obj":"sentences"},{"subj":"T47","pred":"source","obj":"sentences"},{"subj":"T48","pred":"source","obj":"sentences"},{"subj":"T49","pred":"source","obj":"sentences"},{"subj":"T50","pred":"source","obj":"sentences"},{"subj":"T51","pred":"source","obj":"sentences"},{"subj":"T52","pred":"source","obj":"sentences"},{"subj":"T53","pred":"source","obj":"sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#d993ec","default":true}]}]}}