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PMC:1447668 / 51538-52559 JSONTXT

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bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T22680 54-60 Protein denotes CREB-1
T22681 65-70 Protein denotes ATF-2
T22682 302-306 Protein denotes EGFP
T22683 311-316 Protein denotes Foxp3
T22684 317-327 Gene_expression denotes expression
T22685 317-327 Gene_expression denotes expression
T22686 683-686 Protein denotes ATF
T22687 687-691 Protein denotes CREB
R17272 T22682 T22684 themeOf EGFP,expression
R17273 T22683 T22685 themeOf Foxp3,expression

sentences

Id Subject Object Predicate Lexical cue
T22264 0-53 Sentence denotes Transcription factor DNA-binding analysis (TF-ELISA).
T22265 54-215 Sentence denotes CREB-1 and ATF-2 DNA-binding activity was analyzed with the TransFactor Profiling (Inflammation 1) Kit (BD Biosciences) according to the manufacturer's protocol.
T22266 216-456 Sentence denotes Nuclear extracts were prepared from HEK 293T cells transfected with a control vector (EGFP) or Foxp3 expression vector (1,000 ng) in the presence or absence of forskolin (10 μM for 4 h) using the TransFactor Extraction Kit (BD Biosciences).
T22267 457-546 Sentence denotes Protein concentration was determined using a Biophotometer (Eppendorf, Hamburg, Germany).
T22268 547-724 Sentence denotes Nuclear extracts (20 μg) were incubated in preblocked wells containing plate-bound double-stranded oligonucleotides corresponding to an ATF/CREB consensus sequence (…TGACATCA…).
T22269 725-905 Sentence denotes Wells were washed, incubated with the appropriate primary antibody, washed, incubated with secondary antibody (HRP-labeled), washed again, and finally developed with TMB substrate.
T22270 906-1021 Sentence denotes Colorimetric reactions were read using a VersaMax microplate reader (Molecular Devices) at an absorbance of 655 nm.
T294 0-53 Sentence denotes Transcription factor DNA-binding analysis (TF-ELISA).
T295 54-215 Sentence denotes CREB-1 and ATF-2 DNA-binding activity was analyzed with the TransFactor Profiling (Inflammation 1) Kit (BD Biosciences) according to the manufacturer's protocol.
T296 216-456 Sentence denotes Nuclear extracts were prepared from HEK 293T cells transfected with a control vector (EGFP) or Foxp3 expression vector (1,000 ng) in the presence or absence of forskolin (10 μM for 4 h) using the TransFactor Extraction Kit (BD Biosciences).
T297 457-546 Sentence denotes Protein concentration was determined using a Biophotometer (Eppendorf, Hamburg, Germany).
T298 547-724 Sentence denotes Nuclear extracts (20 μg) were incubated in preblocked wells containing plate-bound double-stranded oligonucleotides corresponding to an ATF/CREB consensus sequence (…TGACATCA…).
T299 725-905 Sentence denotes Wells were washed, incubated with the appropriate primary antibody, washed, incubated with secondary antibody (HRP-labeled), washed again, and finally developed with TMB substrate.
T300 906-1021 Sentence denotes Colorimetric reactions were read using a VersaMax microplate reader (Molecular Devices) at an absorbance of 655 nm.

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T22446 0-13 NN denotes Transcription
T22447 14-20 NN denotes factor
T22448 21-32 JJ denotes DNA-binding
T22449 33-41 NN denotes analysis
T22450 42-43 -LRB- denotes (
T22451 43-51 NN denotes TF-ELISA
T22452 51-52 -RRB- denotes )
T22453 54-60 NN denotes CREB-1
T22454 61-64 CC denotes and
T22455 65-70 NN denotes ATF-2
T22456 71-82 JJ denotes DNA-binding
T22457 83-91 NN denotes activity
T22458 92-95 VB denotes was
T22459 96-104 VB denotes analyzed
T22460 105-109 IN denotes with
T22461 110-113 DT denotes the
T22462 114-125 NN denotes TransFactor
T22463 126-135 NN denotes Profiling
T22464 136-137 -LRB- denotes (
T22465 137-149 NN denotes Inflammation
T22466 150-151 CD denotes 1
T22467 151-152 -RRB- denotes )
T22468 153-156 NN denotes Kit
T22469 157-158 -LRB- denotes (
T22470 158-160 NN denotes BD
T22471 161-172 NNP denotes Biosciences
T22472 172-173 -RRB- denotes )
T22473 174-183 VB denotes according
T22474 184-186 TO denotes to
T22475 187-190 DT denotes the
T22476 191-203 NN denotes manufacturer
T22477 203-205 POS denotes 's
T22478 206-214 NN denotes protocol
T22479 216-223 JJ denotes Nuclear
T22480 224-232 NN denotes extracts
T22481 233-237 VB denotes were
T22482 238-246 VB denotes prepared
T22483 247-251 IN denotes from
T22484 252-255 NN denotes HEK
T22485 256-260 NN denotes 293T
T22486 261-266 NN denotes cells
T22487 267-278 VB denotes transfected
T22488 279-283 IN denotes with
T22489 284-285 DT denotes a
T22490 286-293 NN denotes control
T22491 294-300 NN denotes vector
T22492 301-302 -LRB- denotes (
T22493 302-306 NN denotes EGFP
T22494 306-307 -RRB- denotes )
T22495 308-310 CC denotes or
T22496 311-316 NN denotes Foxp3
T22497 317-327 NN denotes expression
T22498 328-334 NN denotes vector
T22499 335-336 -LRB- denotes (
T22500 336-341 CD denotes 1,000
T22501 342-344 NN denotes ng
T22502 344-345 -RRB- denotes )
T22503 346-348 IN denotes in
T22504 349-352 DT denotes the
T22505 353-361 NN denotes presence
T22506 362-364 CC denotes or
T22507 365-372 NN denotes absence
T22508 373-375 IN denotes of
T22509 376-385 NN denotes forskolin
T22510 386-387 -LRB- denotes (
T22511 387-389 CD denotes 10
T22512 390-392 NN denotes μM
T22513 393-396 IN denotes for
T22514 397-398 CD denotes 4
T22515 399-400 NN denotes h
T22516 400-401 -RRB- denotes )
T22517 402-407 VB denotes using
T22518 408-411 DT denotes the
T22519 412-423 NNP denotes TransFactor
T22520 424-434 NN denotes Extraction
T22521 435-438 NN denotes Kit
T22522 439-440 -LRB- denotes (
T22523 440-442 NN denotes BD
T22524 443-454 NNP denotes Biosciences
T22525 454-455 -RRB- denotes )
T22526 457-464 NN denotes Protein
T22527 465-478 NN denotes concentration
T22528 479-482 VB denotes was
T22529 483-493 VB denotes determined
T22530 494-499 VB denotes using
T22531 500-501 DT denotes a
T22532 502-515 NNP denotes Biophotometer
T22533 516-517 -LRB- denotes (
T22534 517-526 NNP denotes Eppendorf
T22535 526-527 -COMMA- denotes ,
T22536 528-535 NNP denotes Hamburg
T22537 535-536 -COMMA- denotes ,
T22538 537-544 NNP denotes Germany
T22539 544-545 -RRB- denotes )
T22540 547-554 JJ denotes Nuclear
T22541 555-563 NN denotes extracts
T22542 564-565 -LRB- denotes (
T22543 565-567 CD denotes 20
T22544 568-570 NN denotes μg
T22545 570-571 -RRB- denotes )
T22546 572-576 VB denotes were
T22547 577-586 VB denotes incubated
T22548 587-589 IN denotes in
T22549 590-600 VB denotes preblocked
T22550 601-606 NN denotes wells
T22551 607-617 VB denotes containing
T22552 618-629 JJ denotes plate-bound
T22553 630-645 JJ denotes double-stranded
T22554 646-662 NN denotes oligonucleotides
T22555 663-676 VB denotes corresponding
T22556 677-679 TO denotes to
T22557 680-682 DT denotes an
T22558 683-691 NN denotes ATF/CREB
T22559 692-701 NN denotes consensus
T22560 702-710 NN denotes sequence
T22561 711-712 -LRB- denotes (
T22562 712-722 NN denotes …TGACATCA…
T22563 722-723 -RRB- denotes )
T22564 725-730 NN denotes Wells
T22565 731-735 VB denotes were
T22566 736-742 VB denotes washed
T22567 742-743 -COMMA- denotes ,
T22568 744-753 VB denotes incubated
T22569 754-758 IN denotes with
T22570 759-762 DT denotes the
T22571 763-774 JJ denotes appropriate
T22572 775-782 JJ denotes primary
T22573 783-791 NN denotes antibody
T22574 791-792 -COMMA- denotes ,
T22575 793-799 VB denotes washed
T22576 799-800 -COMMA- denotes ,
T22577 801-810 VB denotes incubated
T22578 811-815 IN denotes with
T22579 816-825 JJ denotes secondary
T22580 826-834 NN denotes antibody
T22581 835-836 -LRB- denotes (
T22582 836-847 JJ denotes HRP-labeled
T22583 847-848 -RRB- denotes )
T22584 848-849 -COMMA- denotes ,
T22585 850-856 VB denotes washed
T22586 857-862 RB denotes again
T22587 862-863 -COMMA- denotes ,
T22588 864-867 CC denotes and
T22589 868-875 RB denotes finally
T22590 876-885 VB denotes developed
T22591 886-890 IN denotes with
T22592 891-894 NN denotes TMB
T22593 895-904 NN denotes substrate
T22594 906-918 JJ denotes Colorimetric
T22595 919-928 NN denotes reactions
T22596 929-933 VB denotes were
T22597 934-938 VB denotes read
T22598 939-944 VB denotes using
T22599 945-946 DT denotes a
T22600 947-955 NN denotes VersaMax
T22601 956-966 NN denotes microplate
T22602 967-973 NN denotes reader
T22603 974-975 -LRB- denotes (
T22604 975-984 NNP denotes Molecular
T22605 985-992 NNP denotes Devices
T22606 992-993 -RRB- denotes )
T22607 994-996 IN denotes at
T22608 997-999 DT denotes an
T22609 1000-1010 NN denotes absorbance
T22610 1011-1013 IN denotes of
T22611 1014-1017 CD denotes 655
T22612 1018-1020 NN denotes nm
R17082 T22449 T22446 arg1Of analysis,Transcription
R17083 T22449 T22447 arg1Of analysis,factor
R17084 T22449 T22448 arg1Of analysis,DNA-binding
R17085 T22449 T22450 arg1Of analysis,(
R17086 T22451 T22450 arg2Of TF-ELISA,(
R17087 T22452 T22450 arg3Of ),(
R17088 T22453 T22454 arg1Of CREB-1,and
R17089 T22455 T22454 arg2Of ATF-2,and
R17090 T22457 T22453 arg1Of activity,CREB-1
R17091 T22457 T22455 arg1Of activity,ATF-2
R17092 T22457 T22456 arg1Of activity,DNA-binding
R17093 T22457 T22458 arg1Of activity,was
R17094 T22457 T22459 arg2Of activity,analyzed
R17095 T22459 T22458 arg2Of analyzed,was
R17096 T22459 T22460 arg1Of analyzed,with
R17097 T22459 T22473 arg1Of analyzed,according
R17098 T22465 T22464 arg2Of Inflammation,(
R17099 T22465 T22466 arg1Of Inflammation,1
R17100 T22467 T22464 arg3Of ),(
R17101 T22468 T22460 arg2Of Kit,with
R17102 T22468 T22461 arg1Of Kit,the
R17103 T22468 T22462 arg1Of Kit,TransFactor
R17104 T22468 T22463 arg1Of Kit,Profiling
R17105 T22468 T22464 arg1Of Kit,(
R17106 T22468 T22469 arg1Of Kit,(
R17107 T22470 T22469 arg2Of BD,(
R17108 T22470 T22471 arg1Of BD,Biosciences
R17109 T22472 T22469 arg3Of ),(
R17110 T22474 T22473 arg2Of to,according
R17111 T22476 T22475 arg1Of manufacturer,the
R17112 T22476 T22477 arg2Of manufacturer,'s
R17113 T22478 T22474 arg2Of protocol,to
R17114 T22478 T22477 arg1Of protocol,'s
R17115 T22480 T22479 arg1Of extracts,Nuclear
R17116 T22480 T22481 arg1Of extracts,were
R17117 T22480 T22482 arg2Of extracts,prepared
R17118 T22480 T22517 arg1Of extracts,using
R17119 T22482 T22481 arg2Of prepared,were
R17120 T22482 T22483 arg1Of prepared,from
R17121 T22482 T22517 modOf prepared,using
R17122 T22486 T22484 arg1Of cells,HEK
R17123 T22486 T22485 arg1Of cells,293T
R17124 T22486 T22487 arg2Of cells,transfected
R17125 T22486 T22495 arg1Of cells,or
R17126 T22487 T22488 arg1Of transfected,with
R17127 T22491 T22488 arg2Of vector,with
R17128 T22491 T22489 arg1Of vector,a
R17129 T22491 T22490 arg1Of vector,control
R17130 T22491 T22492 arg1Of vector,(
R17131 T22493 T22492 arg2Of EGFP,(
R17132 T22494 T22492 arg3Of ),(
R17133 T22495 T22483 arg2Of or,from
R17134 T22495 T22503 arg1Of or,in
R17135 T22495 T22510 arg1Of or,(
R17136 T22498 T22495 arg2Of vector,or
R17137 T22498 T22496 arg1Of vector,Foxp3
R17138 T22498 T22497 arg1Of vector,expression
R17139 T22498 T22499 arg1Of vector,(
R17140 T22501 T22499 arg2Of ng,(
R17141 T22501 T22500 arg1Of ng,"1,000"
R17142 T22502 T22499 arg3Of ),(
R17143 T22505 T22506 arg1Of presence,or
R17144 T22506 T22503 arg2Of or,in
R17145 T22506 T22504 arg1Of or,the
R17146 T22506 T22508 arg1Of or,of
R17147 T22507 T22506 arg2Of absence,or
R17148 T22509 T22508 arg2Of forskolin,of
R17149 T22512 T22510 arg2Of μM,(
R17150 T22512 T22511 arg1Of μM,10
R17151 T22512 T22513 arg1Of μM,for
R17152 T22515 T22513 arg2Of h,for
R17153 T22515 T22514 arg1Of h,4
R17154 T22516 T22510 arg3Of ),(
R17155 T22521 T22517 arg2Of Kit,using
R17156 T22521 T22518 arg1Of Kit,the
R17157 T22521 T22519 arg1Of Kit,TransFactor
R17158 T22521 T22520 arg1Of Kit,Extraction
R17159 T22521 T22522 arg1Of Kit,(
R17160 T22524 T22522 arg2Of Biosciences,(
R17161 T22524 T22523 arg1Of Biosciences,BD
R17162 T22525 T22522 arg3Of ),(
R17163 T22527 T22526 arg1Of concentration,Protein
R17164 T22527 T22528 arg1Of concentration,was
R17165 T22527 T22529 arg2Of concentration,determined
R17166 T22529 T22528 arg2Of determined,was
R17167 T22530 T22529 arg3Of using,determined
R17168 T22532 T22530 arg2Of Biophotometer,using
R17169 T22532 T22531 arg1Of Biophotometer,a
R17170 T22532 T22533 arg1Of Biophotometer,(
R17171 T22534 T22535 arg1Of Eppendorf,","
R17172 T22535 T22533 arg2Of ",",(
R17173 T22535 T22537 arg1Of ",",","
R17174 T22536 T22535 arg2Of Hamburg,","
R17175 T22538 T22537 arg2Of Germany,","
R17176 T22539 T22533 arg3Of ),(
R17177 T22541 T22540 arg1Of extracts,Nuclear
R17178 T22541 T22542 arg1Of extracts,(
R17179 T22541 T22546 arg1Of extracts,were
R17180 T22541 T22547 arg2Of extracts,incubated
R17181 T22544 T22542 arg2Of μg,(
R17182 T22544 T22543 arg1Of μg,20
R17183 T22545 T22542 arg3Of ),(
R17184 T22547 T22546 arg2Of incubated,were
R17185 T22547 T22548 arg1Of incubated,in
R17186 T22550 T22548 arg2Of wells,in
R17187 T22550 T22549 arg2Of wells,preblocked
R17188 T22550 T22551 arg1Of wells,containing
R17189 T22554 T22551 arg2Of oligonucleotides,containing
R17190 T22554 T22552 arg1Of oligonucleotides,plate-bound
R17191 T22554 T22553 arg1Of oligonucleotides,double-stranded
R17192 T22554 T22555 arg1Of oligonucleotides,corresponding
R17193 T22555 T22556 arg1Of corresponding,to
R17194 T22560 T22556 arg2Of sequence,to
R17195 T22560 T22557 arg1Of sequence,an
R17196 T22560 T22558 arg1Of sequence,ATF/CREB
R17197 T22560 T22559 arg1Of sequence,consensus
R17198 T22560 T22561 arg1Of sequence,(
R17199 T22562 T22561 arg2Of …TGACATCA…,(
R17200 T22563 T22561 arg3Of ),(
R17201 T22564 T22565 arg1Of Wells,were
R17202 T22564 T22566 arg2Of Wells,washed
R17203 T22564 T22568 arg2Of Wells,incubated
R17204 T22564 T22577 arg2Of Wells,incubated
R17205 T22564 T22585 arg1Of Wells,washed
R17206 T22564 T22590 arg2Of Wells,developed
R17207 T22566 T22567 arg1Of washed,","
R17208 T22567 T22576 arg1Of ",",","
R17209 T22568 T22567 arg2Of incubated,","
R17210 T22568 T22569 arg1Of incubated,with
R17211 T22573 T22569 arg2Of antibody,with
R17212 T22573 T22570 arg1Of antibody,the
R17213 T22573 T22571 arg1Of antibody,appropriate
R17214 T22573 T22572 arg1Of antibody,primary
R17215 T22573 T22574 arg1Of antibody,","
R17216 T22573 T22575 arg2Of antibody,washed
R17217 T22576 T22565 arg2Of ",",were
R17218 T22576 T22584 arg1Of ",",","
R17219 T22577 T22576 arg2Of incubated,","
R17220 T22577 T22578 arg1Of incubated,with
R17221 T22580 T22578 arg2Of antibody,with
R17222 T22580 T22579 arg1Of antibody,secondary
R17223 T22580 T22581 arg1Of antibody,(
R17224 T22582 T22581 arg2Of HRP-labeled,(
R17225 T22583 T22581 arg3Of ),(
R17226 T22584 T22587 arg1Of ",",","
R17227 T22584 T22588 arg1Of ",",and
R17228 T22585 T22584 arg2Of washed,","
R17229 T22585 T22586 arg1Of washed,again
R17230 T22590 T22588 arg2Of developed,and
R17231 T22590 T22589 arg1Of developed,finally
R17232 T22590 T22591 arg1Of developed,with
R17233 T22593 T22591 arg2Of substrate,with
R17234 T22593 T22592 arg1Of substrate,TMB
R17235 T22595 T22594 arg1Of reactions,Colorimetric
R17236 T22595 T22596 arg1Of reactions,were
R17237 T22595 T22597 arg2Of reactions,read
R17238 T22597 T22596 arg2Of read,were
R17239 T22597 T22598 modOf read,using
R17240 T22598 T22607 arg1Of using,at
R17241 T22602 T22598 arg2Of reader,using
R17242 T22602 T22599 arg1Of reader,a
R17243 T22602 T22600 arg1Of reader,VersaMax
R17244 T22602 T22601 arg1Of reader,microplate
R17245 T22602 T22603 arg1Of reader,(
R17246 T22605 T22603 arg2Of Devices,(
R17247 T22605 T22604 arg1Of Devices,Molecular
R17248 T22606 T22603 arg3Of ),(
R17249 T22609 T22607 arg2Of absorbance,at
R17250 T22609 T22608 arg1Of absorbance,an
R17251 T22609 T22610 arg1Of absorbance,of
R17252 T22612 T22610 arg2Of nm,of
R17253 T22612 T22611 arg1Of nm,655

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T22272 0-13 NNP denotes Transcription
T22273 14-20 NN denotes factor
T22274 21-32 JJ denotes DNA-binding
T22275 33-41 NN denotes analysis
T22276 42-43 -LRB- denotes (
T22277 43-51 NNP denotes TF-ELISA
T22278 51-52 -RRB- denotes )
T22279 52-53 . denotes .
T22280 54-60 CD denotes CREB-1
T22281 61-64 CC denotes and
T22282 65-70 NNP denotes ATF-2
T22283 71-82 JJ denotes DNA-binding
T22284 83-91 NN denotes activity
T22285 92-95 VBD denotes was
T22286 96-104 VBN denotes analyzed
T22287 105-109 IN denotes with
T22288 110-113 DT denotes the
T22289 114-125 NNP denotes TransFactor
T22290 126-135 NNP denotes Profiling
T22291 136-137 -LRB- denotes (
T22292 137-149 NNP denotes Inflammation
T22293 150-151 CD denotes 1
T22294 151-152 -RRB- denotes )
T22295 153-156 NN denotes Kit
T22296 157-158 -LRB- denotes (
T22297 158-160 NNP denotes BD
T22298 161-172 NNPS denotes Biosciences
T22299 172-173 -RRB- denotes )
T22300 174-183 VBG denotes according
T22301 184-186 TO denotes to
T22302 187-190 DT denotes the
T22303 191-203 NN denotes manufacturer
T22304 203-205 POS denotes 's
T22305 206-214 NN denotes protocol
T22306 214-215 . denotes .
T22307 216-223 NNP denotes Nuclear
T22308 224-232 NNS denotes extracts
T22309 233-237 VBD denotes were
T22310 238-246 VBN denotes prepared
T22311 247-251 IN denotes from
T22312 252-255 NNP denotes HEK
T22313 256-260 CD denotes 293T
T22314 261-266 NNS denotes cells
T22315 267-278 VBN denotes transfected
T22316 279-283 IN denotes with
T22317 284-285 DT denotes a
T22318 286-293 NN denotes control
T22319 294-300 NN denotes vector
T22320 301-302 -LRB- denotes (
T22321 302-306 NNP denotes EGFP
T22322 306-307 -RRB- denotes )
T22323 308-310 CC denotes or
T22324 311-316 JJ denotes Foxp3
T22325 317-327 NN denotes expression
T22326 328-334 NN denotes vector
T22327 335-336 -LRB- denotes (
T22328 336-341 CD denotes 1,000
T22329 342-344 NN denotes ng
T22330 344-345 -RRB- denotes )
T22331 346-348 IN denotes in
T22332 349-352 DT denotes the
T22333 353-361 NN denotes presence
T22334 362-364 CC denotes or
T22335 365-372 NN denotes absence
T22336 373-375 IN denotes of
T22337 376-385 NN denotes forskolin
T22338 386-387 -LRB- denotes (
T22339 387-389 CD denotes 10
T22340 390-392 NN denotes μM
T22341 393-396 IN denotes for
T22342 397-398 CD denotes 4
T22343 399-400 NN denotes h
T22344 400-401 -RRB- denotes )
T22345 402-407 VBG denotes using
T22346 408-411 DT denotes the
T22347 412-423 NNP denotes TransFactor
T22348 424-434 NNP denotes Extraction
T22349 435-438 NNP denotes Kit
T22350 439-440 -LRB- denotes (
T22351 440-442 NNP denotes BD
T22352 443-454 NNPS denotes Biosciences
T22353 454-455 -RRB- denotes )
T22354 455-456 . denotes .
T22355 457-464 NN denotes Protein
T22356 465-478 NN denotes concentration
T22357 479-482 VBD denotes was
T22358 483-493 VBN denotes determined
T22359 494-499 VBG denotes using
T22360 500-501 DT denotes a
T22361 502-515 NNP denotes Biophotometer
T22362 516-517 -LRB- denotes (
T22363 517-526 NNP denotes Eppendorf
T22364 526-527 , denotes ,
T22365 528-535 NNP denotes Hamburg
T22366 535-536 , denotes ,
T22367 537-544 NNP denotes Germany
T22368 544-545 -RRB- denotes )
T22369 545-546 . denotes .
T22370 547-554 NNP denotes Nuclear
T22371 555-563 NNS denotes extracts
T22372 564-565 -LRB- denotes (
T22373 565-567 CD denotes 20
T22374 568-570 NN denotes μg
T22375 570-571 -RRB- denotes )
T22376 572-576 VBD denotes were
T22377 577-586 VBN denotes incubated
T22378 587-589 IN denotes in
T22379 590-600 JJ denotes preblocked
T22380 601-606 NNS denotes wells
T22381 607-617 VBG denotes containing
T22382 618-629 JJ denotes plate-bound
T22383 630-645 JJ denotes double-stranded
T22384 646-662 NNS denotes oligonucleotides
T22385 663-676 JJ denotes corresponding
T22386 677-679 TO denotes to
T22387 680-682 DT denotes an
T22388 683-691 NNP denotes ATF/CREB
T22389 692-701 NN denotes consensus
T22390 702-710 NN denotes sequence
T22391 711-712 -LRB- denotes (
T22392 713-721 NNP denotes TGACATCA
T22393 722-723 -RRB- denotes )
T22394 723-724 . denotes .
T22395 725-730 NNP denotes Wells
T22396 731-735 VBD denotes were
T22397 736-742 VBN denotes washed
T22398 742-743 , denotes ,
T22399 744-753 VBN denotes incubated
T22400 754-758 IN denotes with
T22401 759-762 DT denotes the
T22402 763-774 JJ denotes appropriate
T22403 775-782 JJ denotes primary
T22404 783-791 NN denotes antibody
T22405 791-792 , denotes ,
T22406 793-799 VBD denotes washed
T22407 799-800 , denotes ,
T22408 801-810 VBN denotes incubated
T22409 811-815 IN denotes with
T22410 816-825 JJ denotes secondary
T22411 826-834 NN denotes antibody
T22412 835-836 -LRB- denotes (
T22413 836-847 JJ denotes HRP-labeled
T22414 847-848 -RRB- denotes )
T22415 848-849 , denotes ,
T22416 850-856 VBD denotes washed
T22417 857-862 RB denotes again
T22418 862-863 , denotes ,
T22419 864-867 CC denotes and
T22420 868-875 RB denotes finally
T22421 876-885 VBN denotes developed
T22422 886-890 IN denotes with
T22423 891-894 NNP denotes TMB
T22424 895-904 NN denotes substrate
T22425 904-905 . denotes .
T22426 906-918 JJ denotes Colorimetric
T22427 919-928 NNS denotes reactions
T22428 929-933 VBD denotes were
T22429 934-938 VBN denotes read
T22430 939-944 VBG denotes using
T22431 945-946 DT denotes a
T22432 947-955 NNP denotes VersaMax
T22433 956-966 NN denotes microplate
T22434 967-973 NN denotes reader
T22435 974-975 -LRB- denotes (
T22436 975-984 JJ denotes Molecular
T22437 985-992 NNPS denotes Devices
T22438 992-993 -RRB- denotes )
T22439 994-996 IN denotes at
T22440 997-999 DT denotes an
T22441 1000-1010 NN denotes absorbance
T22442 1011-1013 IN denotes of
T22443 1014-1017 CD denotes 655
T22444 1018-1020 NN denotes nm
T22445 1020-1021 . denotes .
R16908 T22272 T22273 compound Transcription,factor
R16909 T22273 T22273 ROOT factor,factor
R16910 T22274 T22275 amod DNA-binding,analysis
R16911 T22275 T22273 appos analysis,factor
R16912 T22276 T22275 punct (,analysis
R16913 T22277 T22275 appos TF-ELISA,analysis
R16914 T22278 T22275 punct ),analysis
R16915 T22279 T22273 punct .,factor
R16916 T22280 T22284 nummod CREB-1,activity
R16917 T22281 T22280 cc and,CREB-1
R16918 T22282 T22284 nmod ATF-2,activity
R16919 T22283 T22284 amod DNA-binding,activity
R16920 T22284 T22286 nsubjpass activity,analyzed
R16921 T22285 T22286 auxpass was,analyzed
R16922 T22286 T22286 ROOT analyzed,analyzed
R16923 T22287 T22286 prep with,analyzed
R16924 T22288 T22290 det the,Profiling
R16925 T22289 T22290 compound TransFactor,Profiling
R16926 T22290 T22287 pobj Profiling,with
R16927 T22291 T22290 punct (,Profiling
R16928 T22292 T22290 appos Inflammation,Profiling
R16929 T22293 T22292 nummod 1,Inflammation
R16930 T22294 T22290 punct ),Profiling
R16931 T22295 T22300 npadvmod Kit,according
R16932 T22296 T22298 punct (,Biosciences
R16933 T22297 T22298 compound BD,Biosciences
R16934 T22298 T22295 appos Biosciences,Kit
R16935 T22299 T22298 punct ),Biosciences
R16936 T22300 T22286 prep according,analyzed
R16937 T22301 T22300 prep to,according
R16938 T22302 T22303 det the,manufacturer
R16939 T22303 T22305 poss manufacturer,protocol
R16940 T22304 T22303 case 's,manufacturer
R16941 T22305 T22301 pobj protocol,to
R16942 T22306 T22286 punct .,analyzed
R16943 T22307 T22308 compound Nuclear,extracts
R16944 T22308 T22310 nsubjpass extracts,prepared
R16945 T22309 T22310 auxpass were,prepared
R16946 T22310 T22310 ROOT prepared,prepared
R16947 T22311 T22310 prep from,prepared
R16948 T22312 T22311 pobj HEK,from
R16949 T22313 T22314 nummod 293T,cells
R16950 T22314 T22310 dobj cells,prepared
R16951 T22315 T22314 acl transfected,cells
R16952 T22316 T22315 prep with,transfected
R16953 T22317 T22319 det a,vector
R16954 T22318 T22319 compound control,vector
R16955 T22319 T22316 pobj vector,with
R16956 T22320 T22319 punct (,vector
R16957 T22321 T22319 appos EGFP,vector
R16958 T22322 T22319 punct ),vector
R16959 T22323 T22319 cc or,vector
R16960 T22324 T22325 amod Foxp3,expression
R16961 T22325 T22326 compound expression,vector
R16962 T22326 T22319 conj vector,vector
R16963 T22327 T22329 punct (,ng
R16964 T22328 T22329 nummod "1,000",ng
R16965 T22329 T22326 appos ng,vector
R16966 T22330 T22329 punct ),ng
R16967 T22331 T22319 prep in,vector
R16968 T22332 T22333 det the,presence
R16969 T22333 T22331 pobj presence,in
R16970 T22334 T22333 cc or,presence
R16971 T22335 T22333 conj absence,presence
R16972 T22336 T22335 prep of,absence
R16973 T22337 T22336 pobj forskolin,of
R16974 T22338 T22337 punct (,forskolin
R16975 T22339 T22340 nummod 10,μM
R16976 T22340 T22337 appos μM,forskolin
R16977 T22341 T22340 prep for,μM
R16978 T22342 T22343 nummod 4,h
R16979 T22343 T22341 pobj h,for
R16980 T22344 T22337 punct ),forskolin
R16981 T22345 T22315 advcl using,transfected
R16982 T22346 T22349 det the,Kit
R16983 T22347 T22349 compound TransFactor,Kit
R16984 T22348 T22349 compound Extraction,Kit
R16985 T22349 T22345 dobj Kit,using
R16986 T22350 T22352 punct (,Biosciences
R16987 T22351 T22352 compound BD,Biosciences
R16988 T22352 T22349 appos Biosciences,Kit
R16989 T22353 T22349 punct ),Kit
R16990 T22354 T22310 punct .,prepared
R16991 T22355 T22356 compound Protein,concentration
R17021 T22385 T22380 amod corresponding,wells
R17022 T22386 T22385 prep to,corresponding
R17023 T22387 T22390 det an,sequence
R17024 T22388 T22389 compound ATF/CREB,consensus
R17025 T22389 T22390 compound consensus,sequence
R17026 T22390 T22386 pobj sequence,to
R17027 T22391 T22390 punct (,sequence
R17028 T22392 T22390 appos TGACATCA,sequence
R17029 T22393 T22377 punct ),incubated
R17030 T22394 T22377 punct .,incubated
R17031 T22395 T22397 nsubjpass Wells,washed
R17032 T22396 T22397 auxpass were,washed
R17033 T22397 T22397 ROOT washed,washed
R17034 T22398 T22397 punct ",",washed
R17035 T22399 T22397 conj incubated,washed
R17036 T22400 T22399 prep with,incubated
R17037 T22401 T22404 det the,antibody
R17038 T22402 T22404 amod appropriate,antibody
R17039 T22403 T22404 amod primary,antibody
R17040 T22404 T22400 pobj antibody,with
R17041 T22405 T22406 punct ",",washed
R17042 T22406 T22397 conj washed,washed
R17043 T22407 T22406 punct ",",washed
R17044 T22408 T22406 intj incubated,washed
R17045 T22409 T22408 prep with,incubated
R17046 T22410 T22411 amod secondary,antibody
R17047 T22411 T22409 pobj antibody,with
R17048 T22412 T22413 punct (,HRP-labeled
R17049 T22413 T22411 appos HRP-labeled,antibody
R17050 T22414 T22408 punct ),incubated
R17051 T22415 T22406 punct ",",washed
R17052 T22416 T22406 conj washed,washed
R17053 T22417 T22416 advmod again,washed
R17054 T22418 T22416 punct ",",washed
R17055 T22419 T22416 cc and,washed
R17056 T22420 T22421 advmod finally,developed
R17057 T22421 T22416 conj developed,washed
R17058 T22422 T22421 prep with,developed
R17059 T22423 T22424 compound TMB,substrate
R17060 T22424 T22422 pobj substrate,with
R17061 T22425 T22406 punct .,washed
R17062 T22426 T22427 amod Colorimetric,reactions
R17063 T22427 T22429 nsubjpass reactions,read
R17064 T22428 T22429 auxpass were,read
R17065 T22429 T22429 ROOT read,read
R17066 T22430 T22429 advcl using,read
R17067 T22431 T22434 det a,reader
R17068 T22432 T22434 nmod VersaMax,reader
R17069 T22433 T22434 compound microplate,reader
R17070 T22434 T22430 dobj reader,using
R17071 T22435 T22434 punct (,reader
R17072 T22436 T22437 compound Molecular,Devices
R17073 T22437 T22434 appos Devices,reader
R17074 T22438 T22434 punct ),reader
R17075 T22439 T22430 prep at,using
R17076 T22440 T22441 det an,absorbance
R17077 T22441 T22439 pobj absorbance,at
R17078 T22442 T22441 prep of,absorbance
R17079 T22443 T22444 nummod 655,nm
R17080 T22444 T22442 pobj nm,of
R17081 T22445 T22429 punct .,read
R16992 T22356 T22358 nsubjpass concentration,determined
R16993 T22357 T22358 auxpass was,determined
R16994 T22358 T22358 ROOT determined,determined
R16995 T22359 T22358 advcl using,determined
R16996 T22360 T22361 det a,Biophotometer
R16997 T22361 T22359 dobj Biophotometer,using
R16998 T22362 T22363 punct (,Eppendorf
R16999 T22363 T22361 appos Eppendorf,Biophotometer
R17000 T22364 T22363 punct ",",Eppendorf
R17001 T22365 T22363 npadvmod Hamburg,Eppendorf
R17002 T22366 T22365 punct ",",Hamburg
R17003 T22367 T22365 appos Germany,Hamburg
R17004 T22368 T22365 punct ),Hamburg
R17005 T22369 T22358 punct .,determined
R17006 T22370 T22371 compound Nuclear,extracts
R17007 T22371 T22377 nsubjpass extracts,incubated
R17008 T22372 T22371 punct (,extracts
R17009 T22373 T22374 nummod 20,μg
R17010 T22374 T22371 appos μg,extracts
R17011 T22375 T22371 punct ),extracts
R17012 T22376 T22377 auxpass were,incubated
R17013 T22377 T22377 ROOT incubated,incubated
R17014 T22378 T22377 prep in,incubated
R17015 T22379 T22380 amod preblocked,wells
R17016 T22380 T22378 pobj wells,in
R17017 T22381 T22380 acl containing,wells
R17018 T22382 T22384 amod plate-bound,oligonucleotides
R17019 T22383 T22384 amod double-stranded,oligonucleotides
R17020 T22384 T22381 dobj oligonucleotides,containing

GO-BP

Id Subject Object Predicate Lexical cue
T22271 0-13 http://purl.obolibrary.org/obo/GO_0006351 denotes Transcription

GO-MF

Id Subject Object Predicate Lexical cue
T22635 21-32 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA-binding
T22636 71-82 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA-binding
T22637 25-32 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T22638 75-82 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T22639 783-791 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T22640 826-834 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody

GO-CC

Id Subject Object Predicate Lexical cue
T22641 261-266 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T22642 783-791 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T22643 826-834 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T22644 783-791 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T22645 826-834 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody

events-check-again

Id Subject Object Predicate Lexical cue Speculation
T22674 54-60 Protein denotes CREB-1
T22675 65-70 Protein denotes ATF-2
T22676 75-82 Binding denotes binding true
T22677 75-82 Binding denotes binding true
T22678 311-316 Protein denotes Foxp3
T22679 317-327 Gene_expression denotes expression true
R17269 T22674 T22677 themeOf CREB-1,binding
R17270 T22675 T22676 themeOf ATF-2,binding
R17271 T22678 T22679 themeOf Foxp3,expression

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Speculation
T22258 54-60 Protein denotes CREB-1
T22259 65-70 Protein denotes ATF-2
T22260 75-82 Binding denotes binding true
T22261 75-82 Binding denotes binding true
T22262 311-316 Protein denotes Foxp3
T22263 317-327 Gene_expression denotes expression true
R16905 T22258 T22261 themeOf CREB-1,binding
R16906 T22259 T22260 themeOf ATF-2,binding
R16907 T22262 T22263 themeOf Foxp3,expression

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T22613 54-58 P16220 denotes CREB
T22614 54-58 Q8TEY5 denotes CREB
T22615 54-58 Q02930 denotes CREB
T22616 54-58 P18848 denotes CREB
T22617 54-58 O43889 denotes CREB
T22618 54-58 P15336 denotes CREB
T22619 54-60 P16220 denotes CREB-1
T22620 65-70 P15336 denotes ATF-2
T22621 311-316 Q9BZS1 denotes Foxp3
T22622 687-691 P18848 denotes CREB
T22623 687-691 P16220 denotes CREB
T22624 687-691 P15336 denotes CREB
T22625 687-691 Q8TEY5 denotes CREB
T22626 687-691 Q02930 denotes CREB
T22627 687-691 O43889 denotes CREB

test2

Id Subject Object Predicate Lexical cue Speculation
T22253 54-60 Protein denotes CREB-1 true
T22254 65-70 Protein denotes ATF-2 true
T22255 75-82 Binding denotes binding true
T22256 311-316 Protein denotes Foxp3
T22257 317-327 Gene_expression denotes expression
R16902 T22253 T22255 themeOf CREB-1,binding
R16903 T22254 T22255 themeOf ATF-2,binding
R16904 T22256 T22257 themeOf Foxp3,expression