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PMC:1447668 / 49570-51536 JSONTXT

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bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T22237 23-31 Protein denotes HEK 293T
T22238 459-472 Protein denotes Mini Protease
T22239 955-960 Protein denotes Foxp3
T22240 987-1023 Protein denotes anti-human Foxp3 polyclonal antibody
T22241 1082-1120 Protein denotes anti-rabbit IgG-HRP secondary antibody
T22242 1189-1194 Protein denotes NF-κB
T22243 1195-1198 Protein denotes p65
T22244 1203-1209 Protein denotes CREB-1
T22245 1332-1336 Protein denotes Beta
T22246 1337-1342 Protein denotes actin

sentences

Id Subject Object Predicate Lexical cue
T21405 0-22 Sentence denotes Western blot analysis.
T21406 23-245 Sentence denotes HEK 293T cells were plated at a density of 5 × 105 cells/well in six-well culture plates (BD Biosciences) 1 d prior to transfection with the appropriate plasmid DNA (2 μg total) using FuGene 6 transfection reagent (Roche).
T21407 246-492 Sentence denotes Cells were harvested 24 h posttransfection for whole-cell lysates in RIPA buffer (50 mM Tris-HCl [pH 7.4], 150 mM NaCl, 1% Igepal (NP-40), 0.5% sodium deoxycholate, 1 mM EDTA, 1 mM DTT, 1 mM PMSF, and 1× Complete Mini Protease Inhibitor [Roche]).
T21408 493-749 Sentence denotes Protein concentration was determined by Lowry assay (Bio-Rad, Hercules, California, United States) and colorimetric reactions were read using a VersaMax microplate reader (Molecular Devices, Sunnyvale, California, United States) at an absorbance of 750 nm.
T21409 750-954 Sentence denotes Size fractionation was performed on 20 μg of protein/sample by SDS-PAGE, and the protein was transferred to nitrocellulose or PVDF membranes and subjected to immunoblotting using the indicated antibodies.
T21410 955-1188 Sentence denotes Foxp3 was detected using rabbit anti-human Foxp3 polyclonal antibody (ab4728 or ab10563; Abcam, Cambridge, United Kingdom) and anti-rabbit IgG-HRP secondary antibody (Cell Signaling Technology, Beverly, Massachusetts, United States).
T21411 1189-1331 Sentence denotes NF-κB p65 and CREB-1 were detected using rabbit anti-human polyclonal (p65) or monoclonal antibody (CREB-1; 48H2) (Cell Signaling Technology).
T21412 1332-1441 Sentence denotes Beta-actin was detected using a mouse monoclonal antibody (AC-15; Sigma, St. Louis, Missouri, United States).
T21413 1442-1770 Sentence denotes For coimmunoprecipitation analysis, cell lysates were precleared with 30 μl of protein A/G plus-agarose beads (Santa Cruz Biotechnology, Santa Cruz, California, United States) and then incubated with mouse monoclonal anti-HA antibody (6E2; 1:100; Cell Signaling Technology) and 30 μl of protein A/G plus-agarose beads overnight.
T21414 1771-1899 Sentence denotes The immunoprecipitates were washed four times with RIPA buffer, resuspended in SDS sample buffer, and heated at 95 °C for 5 min.
T21415 1900-1966 Sentence denotes Proteins were then treated as described for Western blot analysis.
T283 0-22 Sentence denotes Western blot analysis.
T284 23-245 Sentence denotes HEK 293T cells were plated at a density of 5 × 105 cells/well in six-well culture plates (BD Biosciences) 1 d prior to transfection with the appropriate plasmid DNA (2 μg total) using FuGene 6 transfection reagent (Roche).
T285 246-492 Sentence denotes Cells were harvested 24 h posttransfection for whole-cell lysates in RIPA buffer (50 mM Tris-HCl [pH 7.4], 150 mM NaCl, 1% Igepal (NP-40), 0.5% sodium deoxycholate, 1 mM EDTA, 1 mM DTT, 1 mM PMSF, and 1× Complete Mini Protease Inhibitor [Roche]).
T286 493-749 Sentence denotes Protein concentration was determined by Lowry assay (Bio-Rad, Hercules, California, United States) and colorimetric reactions were read using a VersaMax microplate reader (Molecular Devices, Sunnyvale, California, United States) at an absorbance of 750 nm.
T287 750-954 Sentence denotes Size fractionation was performed on 20 μg of protein/sample by SDS-PAGE, and the protein was transferred to nitrocellulose or PVDF membranes and subjected to immunoblotting using the indicated antibodies.
T288 955-1188 Sentence denotes Foxp3 was detected using rabbit anti-human Foxp3 polyclonal antibody (ab4728 or ab10563; Abcam, Cambridge, United Kingdom) and anti-rabbit IgG-HRP secondary antibody (Cell Signaling Technology, Beverly, Massachusetts, United States).
T289 1189-1331 Sentence denotes NF-κB p65 and CREB-1 were detected using rabbit anti-human polyclonal (p65) or monoclonal antibody (CREB-1; 48H2) (Cell Signaling Technology).
T290 1332-1441 Sentence denotes Beta-actin was detected using a mouse monoclonal antibody (AC-15; Sigma, St. Louis, Missouri, United States).
T291 1442-1770 Sentence denotes For coimmunoprecipitation analysis, cell lysates were precleared with 30 μl of protein A/G plus-agarose beads (Santa Cruz Biotechnology, Santa Cruz, California, United States) and then incubated with mouse monoclonal anti-HA antibody (6E2; 1:100; Cell Signaling Technology) and 30 μl of protein A/G plus-agarose beads overnight.
T292 1771-1899 Sentence denotes The immunoprecipitates were washed four times with RIPA buffer, resuspended in SDS sample buffer, and heated at 95 °C for 5 min.
T293 1900-1966 Sentence denotes Proteins were then treated as described for Western blot analysis.

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T21783 0-7 NN denotes Western
T21784 8-12 NN denotes blot
T21785 13-21 NN denotes analysis
T21786 23-26 NN denotes HEK
T21787 27-31 NN denotes 293T
T21788 32-37 NN denotes cells
T21789 38-42 VB denotes were
T21790 43-49 VB denotes plated
T21791 50-52 IN denotes at
T21792 53-54 DT denotes a
T21793 55-62 NN denotes density
T21794 63-65 IN denotes of
T21795 66-67 CD denotes 5
T21796 68-69 IN denotes ×
T21797 70-73 CD denotes 105
T21798 74-84 NN denotes cells/well
T21799 85-87 IN denotes in
T21800 88-96 JJ denotes six-well
T21801 97-104 NN denotes culture
T21802 105-111 NN denotes plates
T21803 112-113 -LRB- denotes (
T21804 113-115 NN denotes BD
T21805 116-127 NNP denotes Biosciences
T21806 127-128 -RRB- denotes )
T21807 129-130 CD denotes 1
T21808 131-132 NN denotes d
T21809 133-138 JJ denotes prior
T21810 139-141 TO denotes to
T21811 142-154 NN denotes transfection
T21812 155-159 IN denotes with
T21813 160-163 DT denotes the
T21814 164-175 JJ denotes appropriate
T21815 176-183 NN denotes plasmid
T21816 184-187 NN denotes DNA
T21817 188-189 -LRB- denotes (
T21818 189-190 CD denotes 2
T21819 191-193 NN denotes μg
T21820 194-199 NN denotes total
T21821 199-200 -RRB- denotes )
T21822 201-206 VB denotes using
T21823 207-213 NN denotes FuGene
T21824 214-215 CD denotes 6
T21825 216-228 NN denotes transfection
T21826 229-236 NN denotes reagent
T21827 237-238 -LRB- denotes (
T21828 238-243 NN denotes Roche
T21829 243-244 -RRB- denotes )
T21830 246-251 NN denotes Cells
T21831 252-256 VB denotes were
T21832 257-266 VB denotes harvested
T21833 267-269 CD denotes 24
T21834 270-271 NN denotes h
T21835 272-288 NN denotes posttransfection
T21836 289-292 IN denotes for
T21837 293-303 JJ denotes whole-cell
T21838 304-311 NN denotes lysates
T21839 312-314 IN denotes in
T21840 315-319 NN denotes RIPA
T21841 320-326 NN denotes buffer
T21842 327-328 -LRB- denotes (
T21843 328-330 CD denotes 50
T21844 331-333 NN denotes mM
T21845 334-342 NN denotes Tris-HCl
T21846 343-344 -LRB- denotes [
T21847 344-346 NN denotes pH
T21848 347-350 CD denotes 7.4
T21849 350-351 -RRB- denotes ]
T21850 351-352 -COMMA- denotes ,
T21851 353-356 CD denotes 150
T21852 357-359 NN denotes mM
T21853 360-364 NN denotes NaCl
T21854 364-365 -COMMA- denotes ,
T21855 366-367 CD denotes 1
T21856 367-368 NN denotes %
T21857 369-375 NN denotes Igepal
T21858 376-377 -LRB- denotes (
T21859 377-382 NN denotes NP-40
T21860 382-383 -RRB- denotes )
T21861 383-384 -COMMA- denotes ,
T21862 385-388 CD denotes 0.5
T21863 388-389 NN denotes %
T21864 390-396 NN denotes sodium
T21865 397-409 NN denotes deoxycholate
T21866 409-410 -COMMA- denotes ,
T21867 411-412 CD denotes 1
T21868 413-415 NN denotes mM
T21869 416-420 NN denotes EDTA
T21870 420-421 -COMMA- denotes ,
T21871 422-423 CD denotes 1
T21872 424-426 NN denotes mM
T21873 427-430 NN denotes DTT
T21874 430-431 -COMMA- denotes ,
T21875 432-433 CD denotes 1
T21876 434-436 NN denotes mM
T21877 437-441 NN denotes PMSF
T21878 441-442 -COMMA- denotes ,
T21879 443-446 CC denotes and
T21880 447-449 CD denotes 1×
T21881 450-458 NNP denotes Complete
T21882 459-463 NNP denotes Mini
T21883 464-472 NNP denotes Protease
T21884 473-482 NNP denotes Inhibitor
T21885 483-484 -LRB- denotes [
T21886 484-489 NNP denotes Roche
T21887 489-490 -RRB- denotes ]
T21888 490-491 -RRB- denotes )
T21889 493-500 NN denotes Protein
T21890 501-514 NN denotes concentration
T21891 515-518 VB denotes was
T21892 519-529 VB denotes determined
T21893 530-532 IN denotes by
T21894 533-538 NN denotes Lowry
T21895 539-544 NN denotes assay
T21896 545-546 -LRB- denotes (
T21897 546-553 NNP denotes Bio-Rad
T21898 553-554 -COMMA- denotes ,
T21899 555-563 NNP denotes Hercules
T21900 563-564 -COMMA- denotes ,
T21901 565-575 NNP denotes California
T21902 575-576 -COMMA- denotes ,
T21903 577-583 NNP denotes United
T21904 584-590 NNP denotes States
T21905 590-591 -RRB- denotes )
T21906 592-595 CC denotes and
T21907 596-608 JJ denotes colorimetric
T21908 609-618 NN denotes reactions
T21909 619-623 VB denotes were
T21910 624-628 VB denotes read
T21911 629-634 VB denotes using
T21912 635-636 DT denotes a
T21913 637-645 NN denotes VersaMax
T21914 646-656 NN denotes microplate
T21915 657-663 NN denotes reader
T21916 664-665 -LRB- denotes (
T21917 665-674 NNP denotes Molecular
T21918 675-682 NNP denotes Devices
T21919 682-683 -COMMA- denotes ,
T21920 684-693 NNP denotes Sunnyvale
T21921 693-694 -COMMA- denotes ,
T21922 695-705 NNP denotes California
T21923 705-706 -COMMA- denotes ,
T21924 707-713 NNP denotes United
T21925 714-720 NNP denotes States
T21926 720-721 -RRB- denotes )
T21927 722-724 IN denotes at
T21928 725-727 DT denotes an
T21929 728-738 NN denotes absorbance
T21930 739-741 IN denotes of
T21931 742-745 CD denotes 750
T21932 746-748 NN denotes nm
T21933 750-754 NN denotes Size
T21934 755-768 NN denotes fractionation
T21935 769-772 VB denotes was
T21936 773-782 VB denotes performed
T21937 783-785 IN denotes on
T21938 786-788 CD denotes 20
T21939 789-791 NN denotes μg
T21940 792-794 IN denotes of
T21941 795-809 NN denotes protein/sample
T21942 810-812 IN denotes by
T21943 813-821 NN denotes SDS-PAGE
T21944 821-822 -COMMA- denotes ,
T21945 823-826 CC denotes and
T21946 827-830 DT denotes the
T21947 831-838 NN denotes protein
T21948 839-842 VB denotes was
T21949 843-854 VB denotes transferred
T21950 855-857 TO denotes to
T21951 858-872 NN denotes nitrocellulose
T21952 873-875 CC denotes or
T21953 876-880 NN denotes PVDF
T21954 881-890 NN denotes membranes
T21955 891-894 CC denotes and
T21956 895-904 VB denotes subjected
T21957 905-907 TO denotes to
T21958 908-922 NN denotes immunoblotting
T21959 923-928 VB denotes using
T21960 929-932 DT denotes the
T21961 933-942 JJ denotes indicated
T21962 943-953 NN denotes antibodies
T21963 955-960 NN denotes Foxp3
T21964 961-964 VB denotes was
T21965 965-973 VB denotes detected
T21966 974-979 VB denotes using
T21967 980-986 NN denotes rabbit
T21968 987-997 JJ denotes anti-human
T21969 998-1003 NN denotes Foxp3
T21970 1004-1014 JJ denotes polyclonal
T21971 1015-1023 NN denotes antibody
T21972 1024-1025 -LRB- denotes (
T21973 1025-1031 NN denotes ab4728
T21974 1032-1034 CC denotes or
T21975 1035-1042 NN denotes ab10563
T21976 1042-1043 -COLON- denotes ;
T21977 1044-1049 NNP denotes Abcam
T21978 1049-1050 -COMMA- denotes ,
T21979 1051-1060 NNP denotes Cambridge
T21980 1060-1061 -COMMA- denotes ,
T21981 1062-1068 NNP denotes United
T21982 1069-1076 NNP denotes Kingdom
T21983 1076-1077 -RRB- denotes )
T21984 1078-1081 CC denotes and
T21985 1082-1093 JJ denotes anti-rabbit
T21986 1094-1101 NN denotes IgG-HRP
T21987 1102-1111 JJ denotes secondary
T21988 1112-1120 NN denotes antibody
T21989 1121-1122 -LRB- denotes (
T21990 1122-1126 NNP denotes Cell
T21991 1127-1136 NNP denotes Signaling
T21992 1137-1147 NNP denotes Technology
T21993 1147-1148 -COMMA- denotes ,
T21994 1149-1156 NNP denotes Beverly
T21995 1156-1157 -COMMA- denotes ,
T21996 1158-1171 NNP denotes Massachusetts
T21997 1171-1172 -COMMA- denotes ,
T21998 1173-1179 NNP denotes United
T21999 1180-1186 NNP denotes States
T22000 1186-1187 -RRB- denotes )
T22001 1189-1194 NN denotes NF-κB
T22002 1195-1198 NN denotes p65
T22003 1199-1202 CC denotes and
T22004 1203-1209 NN denotes CREB-1
T22005 1210-1214 VB denotes were
T22006 1215-1223 VB denotes detected
T22007 1224-1229 VB denotes using
T22008 1230-1236 NN denotes rabbit
T22009 1237-1247 JJ denotes anti-human
T22010 1248-1258 JJ denotes polyclonal
T22011 1259-1260 -LRB- denotes (
T22012 1260-1263 NN denotes p65
T22013 1263-1264 -RRB- denotes )
T22014 1265-1267 CC denotes or
T22015 1268-1278 JJ denotes monoclonal
T22016 1279-1287 NN denotes antibody
T22017 1288-1289 -LRB- denotes (
T22018 1289-1295 NN denotes CREB-1
T22019 1295-1296 -COLON- denotes ;
T22020 1297-1301 NN denotes 48H2
T22021 1301-1302 -RRB- denotes )
T22022 1303-1304 -LRB- denotes (
T22023 1304-1308 NNP denotes Cell
T22024 1309-1318 NNP denotes Signaling
T22025 1319-1329 NNP denotes Technology
T22026 1329-1330 -RRB- denotes )
T22027 1332-1342 NN denotes Beta-actin
T22028 1343-1346 VB denotes was
T22029 1347-1355 VB denotes detected
T22030 1356-1361 VB denotes using
T22031 1362-1363 DT denotes a
T22032 1364-1369 NN denotes mouse
T22033 1370-1380 JJ denotes monoclonal
T22034 1381-1389 NN denotes antibody
T22035 1390-1391 -LRB- denotes (
T22036 1391-1396 NN denotes AC-15
T22037 1396-1397 -COLON- denotes ;
T22038 1398-1403 NNP denotes Sigma
T22039 1403-1404 -COMMA- denotes ,
T22040 1405-1408 NNP denotes St.
T22041 1409-1414 NNP denotes Louis
T22042 1414-1415 -COMMA- denotes ,
T22043 1416-1424 NNP denotes Missouri
T22044 1424-1425 -COMMA- denotes ,
T22045 1426-1432 NNP denotes United
T22046 1433-1439 NNP denotes States
T22047 1439-1440 -RRB- denotes )
T22048 1442-1445 IN denotes For
T22049 1446-1467 NN denotes coimmunoprecipitation
T22050 1468-1476 NN denotes analysis
T22051 1476-1477 -COMMA- denotes ,
T22052 1478-1482 NN denotes cell
T22053 1483-1490 NN denotes lysates
T22054 1491-1495 VB denotes were
T22055 1496-1506 VB denotes precleared
T22056 1507-1511 IN denotes with
T22057 1512-1514 CD denotes 30
T22058 1515-1517 NN denotes μl
T22059 1518-1520 IN denotes of
T22060 1521-1528 NN denotes protein
T22061 1529-1532 NN denotes A/G
T22062 1533-1545 JJ denotes plus-agarose
T22063 1546-1551 NN denotes beads
T22064 1552-1553 -LRB- denotes (
T22065 1553-1558 NNP denotes Santa
T22066 1559-1563 NNP denotes Cruz
T22067 1564-1577 NNP denotes Biotechnology
T22068 1577-1578 -COMMA- denotes ,
T22069 1579-1584 NNP denotes Santa
T22070 1585-1589 NNP denotes Cruz
T22071 1589-1590 -COMMA- denotes ,
T22072 1591-1601 NNP denotes California
T22073 1601-1602 -COMMA- denotes ,
T22074 1603-1609 NNP denotes United
T22075 1610-1616 NNP denotes States
T22076 1616-1617 -RRB- denotes )
T22077 1618-1621 CC denotes and
T22078 1622-1626 RB denotes then
T22079 1627-1636 VB denotes incubated
T22080 1637-1641 IN denotes with
T22081 1642-1647 NN denotes mouse
T22082 1648-1658 JJ denotes monoclonal
T22083 1659-1666 JJ denotes anti-HA
T22084 1667-1675 NN denotes antibody
T22085 1676-1677 -LRB- denotes (
T22086 1677-1680 NN denotes 6E2
T22087 1680-1681 -COLON- denotes ;
T22088 1682-1683 CD denotes 1
T22089 1683-1684 -COLON- denotes :
T22090 1684-1687 CD denotes 100
T22091 1687-1688 -COLON- denotes ;
T22092 1689-1693 NNP denotes Cell
T22093 1694-1703 NNP denotes Signaling
T22094 1704-1714 NNP denotes Technology
T22095 1714-1715 -RRB- denotes )
T22096 1716-1719 CC denotes and
T22097 1720-1722 CD denotes 30
T22098 1723-1725 NN denotes μl
T22099 1726-1728 IN denotes of
T22100 1729-1736 NN denotes protein
T22101 1737-1740 NN denotes A/G
T22102 1741-1753 JJ denotes plus-agarose
T22103 1754-1759 NN denotes beads
T22104 1760-1769 RB denotes overnight
T22105 1771-1774 DT denotes The
T22106 1775-1793 NN denotes immunoprecipitates
T22107 1794-1798 VB denotes were
T22108 1799-1805 VB denotes washed
T22109 1806-1810 CD denotes four
T22110 1811-1816 NN denotes times
T22111 1817-1821 IN denotes with
T22112 1822-1826 NN denotes RIPA
T22113 1827-1833 NN denotes buffer
T22114 1833-1834 -COMMA- denotes ,
T22115 1835-1846 VB denotes resuspended
T22116 1847-1849 IN denotes in
T22117 1850-1853 NN denotes SDS
T22118 1854-1860 NN denotes sample
T22119 1861-1867 NN denotes buffer
T22120 1867-1868 -COMMA- denotes ,
T22121 1869-1872 CC denotes and
T22122 1873-1879 VB denotes heated
T22123 1880-1882 IN denotes at
T22124 1883-1885 CD denotes 95
T22125 1886-1888 NN denotes °C
T22126 1889-1892 IN denotes for
T22127 1893-1894 CD denotes 5
T22128 1895-1898 NN denotes min
T22129 1900-1908 NN denotes Proteins
T22130 1909-1913 VB denotes were
T22131 1914-1918 RB denotes then
T22132 1919-1926 VB denotes treated
T22133 1927-1929 IN denotes as
T22134 1930-1939 VB denotes described
T22135 1940-1943 IN denotes for
T22136 1944-1951 JJ denotes Western
T22137 1952-1956 NN denotes blot
T22138 1957-1965 NN denotes analysis
R16533 T21785 T21783 arg1Of analysis,Western
R16534 T21785 T21784 arg1Of analysis,blot
R16535 T21788 T21786 arg1Of cells,HEK
R16536 T21788 T21787 arg1Of cells,293T
R16537 T21788 T21789 arg1Of cells,were
R16538 T21788 T21790 arg2Of cells,plated
R16539 T21788 T21822 arg1Of cells,using
R16540 T21790 T21789 arg2Of plated,were
R16541 T21790 T21791 arg1Of plated,at
R16542 T21790 T21822 modOf plated,using
R16543 T21793 T21791 arg2Of density,at
R16544 T21793 T21792 arg1Of density,a
R16545 T21793 T21794 arg1Of density,of
R16546 T21793 T21799 arg1Of density,in
R16547 T21797 T21796 arg1Of 105,×
R16548 T21798 T21794 arg2Of cells/well,of
R16549 T21798 T21795 arg1Of cells/well,5
R16550 T21798 T21797 arg1Of cells/well,105
R16551 T21802 T21799 arg2Of plates,in
R16552 T21802 T21800 arg1Of plates,six-well
R16553 T21802 T21801 arg1Of plates,culture
R16554 T21802 T21803 arg1Of plates,(
R16555 T21805 T21803 arg2Of Biosciences,(
R16556 T21805 T21804 arg1Of Biosciences,BD
R16557 T21806 T21803 arg3Of ),(
R16558 T21808 T21791 arg3Of d,at
R16559 T21808 T21807 arg1Of d,1
R16560 T21808 T21809 arg1Of d,prior
R16561 T21808 T21812 arg1Of d,with
R16562 T21809 T21810 arg1Of prior,to
R16563 T21811 T21810 arg2Of transfection,to
R16564 T21816 T21812 arg2Of DNA,with
R16565 T21816 T21813 arg1Of DNA,the
R16566 T21816 T21814 arg1Of DNA,appropriate
R16567 T21816 T21815 arg1Of DNA,plasmid
R16568 T21816 T21817 arg1Of DNA,(
R16569 T21820 T21817 arg2Of total,(
R16570 T21820 T21818 arg1Of total,2
R16571 T21820 T21819 arg1Of total,μg
R16572 T21821 T21817 arg3Of ),(
R16573 T21826 T21822 arg2Of reagent,using
R16574 T21826 T21823 arg1Of reagent,FuGene
R16575 T21826 T21824 arg1Of reagent,6
R16576 T21826 T21825 arg1Of reagent,transfection
R16577 T21826 T21827 arg1Of reagent,(
R16578 T21828 T21827 arg2Of Roche,(
R16579 T21829 T21827 arg3Of ),(
R16580 T21830 T21831 arg1Of Cells,were
R16581 T21830 T21832 arg2Of Cells,harvested
R16582 T21832 T21831 arg2Of harvested,were
R16583 T21835 T21832 arg3Of posttransfection,harvested
R16584 T21835 T21833 arg1Of posttransfection,24
R16585 T21835 T21834 arg1Of posttransfection,h
R16586 T21835 T21836 arg1Of posttransfection,for
R16587 T21835 T21839 arg1Of posttransfection,in
R16588 T21835 T21842 arg1Of posttransfection,(
R16589 T21838 T21836 arg2Of lysates,for
R16590 T21838 T21837 arg1Of lysates,whole-cell
R16591 T21841 T21839 arg2Of buffer,in
R16592 T21841 T21840 arg1Of buffer,RIPA
R16593 T21843 T21844 arg1Of 50,mM
R16594 T21845 T21843 arg1Of Tris-HCl,50
R16595 T21845 T21846 arg1Of Tris-HCl,[
R16596 T21845 T21850 arg1Of Tris-HCl,","
R16597 T21847 T21846 arg2Of pH,[
R16598 T21847 T21848 arg1Of pH,7.4
R16599 T21849 T21846 arg3Of ],[
R16600 T21850 T21854 arg1Of ",",","
R16601 T21851 T21852 arg1Of 150,mM
R16602 T21853 T21850 arg2Of NaCl,","
R16603 T21853 T21851 arg1Of NaCl,150
R16604 T21854 T21861 arg1Of ",",","
R16605 T21855 T21856 arg1Of 1,%
R16606 T21857 T21854 arg2Of Igepal,","
R16607 T21857 T21855 arg1Of Igepal,1
R16608 T21857 T21858 arg1Of Igepal,(
R16609 T21859 T21858 arg2Of NP-40,(
R16610 T21860 T21858 arg3Of ),(
R16611 T21861 T21842 arg2Of ",",(
R16612 T21861 T21866 arg1Of ",",","
R16613 T21862 T21863 arg1Of 0.5,%
R16614 T21865 T21861 arg2Of deoxycholate,","
R16615 T21865 T21862 arg1Of deoxycholate,0.5
R16616 T21865 T21864 arg1Of deoxycholate,sodium
R16617 T21867 T21868 arg1Of 1,mM
R16618 T21869 T21867 arg1Of EDTA,1
R16619 T21869 T21870 arg1Of EDTA,","
R16620 T21870 T21874 arg1Of ",",","
R16621 T21871 T21872 arg1Of 1,mM
R16622 T21873 T21870 arg2Of DTT,","
R16623 T21873 T21871 arg1Of DTT,1
R16624 T21874 T21879 arg1Of ",",and
R16625 T21875 T21876 arg1Of 1,mM
R16626 T21877 T21874 arg2Of PMSF,","
R16627 T21877 T21875 arg1Of PMSF,1
R16628 T21879 T21866 arg2Of and,","
R16629 T21879 T21878 arg1Of and,","
R16630 T21884 T21879 arg2Of Inhibitor,and
R16631 T21884 T21880 arg1Of Inhibitor,1×
R16632 T21884 T21881 arg1Of Inhibitor,Complete
R16633 T21884 T21882 arg1Of Inhibitor,Mini
R16634 T21884 T21883 arg1Of Inhibitor,Protease
R16635 T21884 T21885 arg1Of Inhibitor,[
R16636 T21886 T21885 arg2Of Roche,[
R16637 T21887 T21885 arg3Of ],[
R16638 T21888 T21842 arg3Of ),(
R16639 T21890 T21889 arg1Of concentration,Protein
R16640 T21890 T21891 arg1Of concentration,was
R16641 T21890 T21892 arg2Of concentration,determined
R16642 T21892 T21891 arg2Of determined,was
R16643 T21892 T21896 arg1Of determined,(
R16644 T21892 T21906 arg1Of determined,and
R16645 T21895 T21892 arg1Of assay,determined
R16646 T21895 T21893 arg2Of assay,by
R16647 T21895 T21894 arg1Of assay,Lowry
R16648 T21897 T21898 arg1Of Bio-Rad,","
R16649 T21898 T21900 arg1Of ",",","
R16650 T21899 T21898 arg2Of Hercules,","
R16651 T21900 T21902 arg1Of ",",","
R16652 T21901 T21900 arg2Of California,","
R16653 T21902 T21896 arg2Of ",",(
R16654 T21904 T21902 arg2Of States,","
R16655 T21904 T21903 arg1Of States,United
R16656 T21905 T21896 arg3Of ),(
R16657 T21906 T21911 modOf and,using
R16658 T21908 T21907 arg1Of reactions,colorimetric
R16659 T21908 T21909 arg1Of reactions,were
R16660 T21908 T21910 arg2Of reactions,read
R16661 T21910 T21906 arg2Of read,and
R16662 T21910 T21909 arg2Of read,were
R16663 T21911 T21927 arg1Of using,at
R16664 T21915 T21911 arg2Of reader,using
R16665 T21915 T21912 arg1Of reader,a
R16666 T21915 T21913 arg1Of reader,VersaMax
R16667 T21915 T21914 arg1Of reader,microplate
R16668 T21915 T21916 arg1Of reader,(
R16669 T21918 T21917 arg1Of Devices,Molecular
R16670 T21918 T21919 arg1Of Devices,","
R16671 T21919 T21921 arg1Of ",",","
R16672 T21920 T21919 arg2Of Sunnyvale,","
R16673 T21921 T21923 arg1Of ",",","
R16674 T21922 T21921 arg2Of California,","
R16675 T21923 T21916 arg2Of ",",(
R16676 T21925 T21923 arg2Of States,","
R16677 T21925 T21924 arg1Of States,United
R16678 T21926 T21916 arg3Of ),(
R16679 T21929 T21927 arg2Of absorbance,at
R16680 T21929 T21928 arg1Of absorbance,an
R16681 T21929 T21930 arg1Of absorbance,of
R16682 T21932 T21930 arg2Of nm,of
R16683 T21932 T21931 arg1Of nm,750
R16684 T21934 T21933 arg1Of fractionation,Size
R16685 T21934 T21935 arg1Of fractionation,was
R16686 T21934 T21936 arg2Of fractionation,performed
R16687 T21936 T21935 arg2Of performed,was
R16688 T21936 T21937 arg1Of performed,on
R16689 T21936 T21945 arg1Of performed,and
R16690 T21939 T21937 arg2Of μg,on
R16691 T21939 T21938 arg1Of μg,20
R16692 T21939 T21940 arg1Of μg,of
R16693 T21939 T21942 arg1Of μg,by
R16694 T21941 T21940 arg2Of protein/sample,of
R16695 T21943 T21942 arg2Of SDS-PAGE,by
R16696 T21945 T21944 arg1Of and,","
R16697 T21947 T21946 arg1Of protein,the
R16698 T21947 T21948 arg1Of protein,was
R16699 T21947 T21949 arg2Of protein,transferred
R16700 T21947 T21956 arg2Of protein,subjected
R16701 T21949 T21950 arg1Of transferred,to
R16702 T21949 T21955 arg1Of transferred,and
R16703 T21951 T21952 arg1Of nitrocellulose,or
R16704 T21953 T21952 arg2Of PVDF,or
R16705 T21954 T21950 arg2Of membranes,to
R16706 T21954 T21951 arg1Of membranes,nitrocellulose
R16707 T21954 T21953 arg1Of membranes,PVDF
R16708 T21955 T21945 arg2Of and,and
R16709 T21955 T21948 arg2Of and,was
R16710 T21956 T21955 arg2Of subjected,and
R16711 T21956 T21957 arg1Of subjected,to
R16712 T21956 T21959 modOf subjected,using
R16713 T21958 T21957 arg2Of immunoblotting,to
R16714 T21962 T21959 arg2Of antibodies,using
R16715 T21962 T21960 arg1Of antibodies,the
R16716 T21962 T21961 arg1Of antibodies,indicated
R16717 T21963 T21964 arg1Of Foxp3,was
R16718 T21963 T21965 arg2Of Foxp3,detected
R16719 T21965 T21964 arg2Of detected,was
R16720 T21966 T21965 arg3Of using,detected
R16721 T21968 T21967 arg1Of anti-human,rabbit
R16722 T21971 T21968 arg1Of antibody,anti-human
R16723 T21971 T21969 arg1Of antibody,Foxp3
R16724 T21971 T21970 arg1Of antibody,polyclonal
R16725 T21971 T21972 arg1Of antibody,(
R16726 T21971 T21984 arg1Of antibody,and
R16727 T21973 T21974 arg1Of ab4728,or
R16728 T21974 T21972 arg2Of or,(
R16729 T21974 T21976 arg1Of or,;
R16730 T21975 T21974 arg2Of ab10563,or
R16731 T21977 T21976 arg2Of Abcam,;
R16732 T21977 T21978 arg1Of Abcam,","
R16733 T21977 T21980 arg1Of Abcam,","
R16734 T21979 T21978 arg2Of Cambridge,","
R16735 T21982 T21980 arg2Of Kingdom,","
R16736 T21982 T21981 arg1Of Kingdom,United
R16737 T21983 T21972 arg3Of ),(
R16738 T21984 T21966 arg2Of and,using
R16739 T21984 T21989 arg1Of and,(
R16740 T21988 T21984 arg2Of antibody,and
R16741 T21988 T21985 arg1Of antibody,anti-rabbit
R16742 T21988 T21986 arg1Of antibody,IgG-HRP
R16743 T21988 T21987 arg1Of antibody,secondary
R16744 T21992 T21990 arg1Of Technology,Cell
R16745 T21992 T21991 arg1Of Technology,Signaling
R16746 T21992 T21993 arg1Of Technology,","
R16747 T21993 T21995 arg1Of ",",","
R16748 T21994 T21993 arg2Of Beverly,","
R16749 T21995 T21997 arg1Of ",",","
R16750 T21996 T21995 arg2Of Massachusetts,","
R16751 T21998 T21997 arg2Of United,","
R16752 T21999 T21989 arg2Of States,(
R16753 T21999 T21992 arg1Of States,Technology
R16754 T21999 T21994 arg1Of States,Beverly
R16755 T21999 T21996 arg1Of States,Massachusetts
R16756 T21999 T21998 arg1Of States,United
R16757 T22000 T21989 arg3Of ),(
R16758 T22002 T22003 arg1Of p65,and
R16759 T22003 T22001 arg1Of and,NF-κB
R16760 T22003 T22005 arg1Of and,were
R16761 T22003 T22006 arg2Of and,detected
R16762 T22004 T22003 arg2Of CREB-1,and
R16763 T22006 T22005 arg2Of detected,were
R16764 T22007 T22006 arg3Of using,detected
R16765 T22010 T22008 arg1Of polyclonal,rabbit
R16766 T22010 T22009 arg1Of polyclonal,anti-human
R16767 T22012 T22010 arg1Of p65,polyclonal
R16768 T22012 T22011 arg2Of p65,(
R16769 T22012 T22014 arg1Of p65,or
R16770 T22013 T22011 arg3Of ),(
R16771 T22014 T22007 arg2Of or,using
R16772 T22014 T22022 arg1Of or,(
R16773 T22016 T22014 arg2Of antibody,or
R16774 T22016 T22015 arg1Of antibody,monoclonal
R16775 T22016 T22017 arg1Of antibody,(
R16776 T22018 T22017 arg2Of CREB-1,(
R16777 T22018 T22019 arg1Of CREB-1,;
R16778 T22020 T22019 arg2Of 48H2,;
R16779 T22021 T22017 arg3Of ),(
R16780 T22025 T22022 arg2Of Technology,(
R16781 T22025 T22023 arg1Of Technology,Cell
R16782 T22025 T22024 arg1Of Technology,Signaling
R16783 T22026 T22022 arg3Of ),(
R16784 T22027 T22028 arg1Of Beta-actin,was
R16785 T22027 T22029 arg2Of Beta-actin,detected
R16786 T22027 T22030 arg1Of Beta-actin,using
R16787 T22029 T22028 arg2Of detected,was
R16788 T22030 T22029 arg3Of using,detected
R16789 T22034 T22030 arg2Of antibody,using
R16790 T22034 T22031 arg1Of antibody,a
R16791 T22034 T22032 arg1Of antibody,mouse
R16792 T22034 T22033 arg1Of antibody,monoclonal
R16793 T22034 T22035 arg1Of antibody,(
R16794 T22036 T22035 arg2Of AC-15,(
R16795 T22036 T22037 arg1Of AC-15,;
R16796 T22038 T22039 arg1Of Sigma,","
R16797 T22039 T22042 arg1Of ",",","
R16798 T22041 T22039 arg2Of Louis,","
R16799 T22041 T22040 arg1Of Louis,St.
R16800 T22042 T22044 arg1Of ",",","
R16801 T22043 T22042 arg2Of Missouri,","
R16802 T22044 T22037 arg2Of ",",;
R16803 T22046 T22044 arg2Of States,","
R16804 T22046 T22045 arg1Of States,United
R16805 T22047 T22035 arg3Of ),(
R16806 T22050 T22048 arg2Of analysis,For
R16807 T22050 T22049 arg1Of analysis,coimmunoprecipitation
R16808 T22053 T22052 arg1Of lysates,cell
R16809 T22053 T22054 arg1Of lysates,were
R16810 T22053 T22055 arg2Of lysates,precleared
R16811 T22053 T22079 arg2Of lysates,incubated
R16812 T22055 T22056 arg1Of precleared,with
R16813 T22055 T22077 arg1Of precleared,and
R16814 T22058 T22056 arg2Of μl,with
R16815 T22058 T22057 arg1Of μl,30
R16816 T22058 T22059 arg1Of μl,of
R16817 T22063 T22059 arg2Of beads,of
R16818 T22063 T22060 arg1Of beads,protein
R16819 T22063 T22061 arg1Of beads,A/G
R16820 T22063 T22062 arg1Of beads,plus-agarose
R16821 T22063 T22064 arg1Of beads,(
R16822 T22067 T22065 arg1Of Biotechnology,Santa
R16823 T22067 T22066 arg1Of Biotechnology,Cruz
R16824 T22067 T22068 arg1Of Biotechnology,","
R16825 T22068 T22071 arg1Of ",",","
R16826 T22070 T22068 arg2Of Cruz,","
R16827 T22070 T22069 arg1Of Cruz,Santa
R16828 T22071 T22064 arg2Of ",",(
R16829 T22071 T22073 arg1Of ",",","
R16830 T22072 T22071 arg2Of California,","
R16831 T22075 T22073 arg2Of States,","
R16832 T22075 T22074 arg1Of States,United
R16833 T22076 T22064 arg3Of ),(
R16834 T22077 T22048 arg1Of and,For
R16835 T22077 T22051 arg1Of and,","
R16836 T22077 T22054 arg2Of and,were
R16837 T22079 T22077 arg2Of incubated,and
R16838 T22079 T22078 arg1Of incubated,then
R16839 T22079 T22080 arg1Of incubated,with
R16840 T22084 T22081 arg1Of antibody,mouse
R16841 T22084 T22082 arg1Of antibody,monoclonal
R16842 T22084 T22083 arg1Of antibody,anti-HA
R16843 T22084 T22085 arg1Of antibody,(
R16844 T22084 T22096 arg1Of antibody,and
R16845 T22086 T22087 arg1Of 6E2,;
R16846 T22087 T22085 arg2Of ;,(
R16847 T22087 T22089 arg1Of ;,:
R16848 T22088 T22087 arg2Of 1,;
R16849 T22090 T22091 arg1Of 100,;
R16850 T22091 T22089 arg2Of ;,:
R16851 T22094 T22091 arg2Of Technology,;
R16852 T22094 T22092 arg1Of Technology,Cell
R16853 T22094 T22093 arg1Of Technology,Signaling
R16854 T22095 T22085 arg3Of ),(
R16855 T22096 T22080 arg2Of and,with
R16856 T22098 T22096 arg2Of μl,and
R16857 T22098 T22097 arg1Of μl,30
R16858 T22098 T22099 arg1Of μl,of
R16859 T22103 T22099 arg2Of beads,of
R16860 T22103 T22100 arg1Of beads,protein
R16861 T22103 T22101 arg1Of beads,A/G
R16862 T22103 T22102 arg1Of beads,plus-agarose
R16863 T22103 T22104 arg1Of beads,overnight
R16864 T22106 T22105 arg1Of immunoprecipitates,The
R16865 T22106 T22107 arg1Of immunoprecipitates,were
R16866 T22106 T22108 arg2Of immunoprecipitates,washed
R16867 T22106 T22122 arg2Of immunoprecipitates,heated
R16868 T22108 T22121 arg1Of washed,and
R16869 T22110 T22108 arg3Of times,washed
R16870 T22110 T22109 arg1Of times,four
R16871 T22110 T22111 arg1Of times,with
R16872 T22110 T22115 arg2Of times,resuspended
R16873 T22113 T22111 arg2Of buffer,with
R16874 T22113 T22112 arg1Of buffer,RIPA
R16875 T22115 T22114 arg1Of resuspended,","
R16876 T22115 T22116 arg1Of resuspended,in
R16877 T22119 T22116 arg2Of buffer,in
R16878 T22119 T22117 arg1Of buffer,SDS
R16879 T22119 T22118 arg1Of buffer,sample
R16880 T22121 T22107 arg2Of and,were
R16881 T22121 T22120 arg1Of and,","
R16882 T22122 T22121 arg2Of heated,and
R16883 T22122 T22123 arg1Of heated,at
R16884 T22122 T22126 arg1Of heated,for
R16885 T22125 T22123 arg2Of °C,at
R16886 T22125 T22124 arg1Of °C,95
R16887 T22128 T22126 arg2Of min,for
R16888 T22128 T22127 arg1Of min,5
R16889 T22129 T22130 arg1Of Proteins,were
R16890 T22129 T22132 arg2Of Proteins,treated
R16891 T22132 T22130 arg2Of treated,were
R16892 T22132 T22131 arg1Of treated,then
R16893 T22132 T22133 arg1Of treated,as
R16894 T22134 T22133 arg2Of described,as
R16895 T22134 T22135 arg1Of described,for
R16896 T22138 T22135 arg2Of analysis,for
R16897 T22138 T22136 arg1Of analysis,Western
R16898 T22138 T22137 arg1Of analysis,blot

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T21416 0-7 JJ denotes Western
T21417 8-12 NN denotes blot
T21418 13-21 NN denotes analysis
T21419 21-22 . denotes .
T21420 23-26 NNP denotes HEK
T21421 27-31 CD denotes 293T
T21422 32-37 NNS denotes cells
T21423 38-42 VBD denotes were
T21424 43-49 VBN denotes plated
T21425 50-52 IN denotes at
T21426 53-54 DT denotes a
T21427 55-62 NN denotes density
T21428 63-65 IN denotes of
T21429 66-67 CD denotes 5
T21430 68-69 CD denotes ×
T21431 70-73 CD denotes 105
T21432 74-84 NN denotes cells/well
T21433 85-87 IN denotes in
T21434 88-96 JJ denotes six-well
T21435 97-104 NN denotes culture
T21436 105-111 NNS denotes plates
T21437 112-113 -LRB- denotes (
T21438 113-115 NNP denotes BD
T21439 116-127 NNPS denotes Biosciences
T21440 127-128 -RRB- denotes )
T21441 129-130 CD denotes 1
T21442 131-132 LS denotes d
T21443 133-138 RB denotes prior
T21444 139-141 TO denotes to
T21445 142-154 NN denotes transfection
T21446 155-159 IN denotes with
T21447 160-163 DT denotes the
T21448 164-175 JJ denotes appropriate
T21449 176-183 NN denotes plasmid
T21450 184-187 NN denotes DNA
T21451 188-189 -LRB- denotes (
T21452 189-190 CD denotes 2
T21453 191-193 NN denotes μg
T21454 194-199 NN denotes total
T21455 199-200 -RRB- denotes )
T21456 201-206 VBG denotes using
T21457 207-213 NNP denotes FuGene
T21458 214-215 CD denotes 6
T21459 216-228 NN denotes transfection
T21460 229-236 NN denotes reagent
T21461 237-238 -LRB- denotes (
T21462 238-243 NNP denotes Roche
T21463 243-244 -RRB- denotes )
T21464 244-245 . denotes .
T21465 246-251 NNS denotes Cells
T21466 252-256 VBD denotes were
T21467 257-266 VBN denotes harvested
T21468 267-269 CD denotes 24
T21469 270-271 NN denotes h
T21470 272-288 NN denotes posttransfection
T21471 289-292 IN denotes for
T21472 293-303 JJ denotes whole-cell
T21473 304-311 NNS denotes lysates
T21474 312-314 IN denotes in
T21475 315-319 NNP denotes RIPA
T21476 320-326 NN denotes buffer
T21477 327-328 -LRB- denotes (
T21478 328-330 CD denotes 50
T21479 331-333 NNP denotes mM
T21480 334-342 NNP denotes Tris-HCl
T21481 343-344 NNP denotes [
T21482 344-346 NNP denotes pH
T21483 347-350 CD denotes 7.4
T21484 350-351 NNP denotes ]
T21485 351-352 , denotes ,
T21486 353-356 CD denotes 150
T21487 357-359 NNP denotes mM
T21488 360-364 NNP denotes NaCl
T21489 364-365 , denotes ,
T21490 366-367 CD denotes 1
T21491 367-368 NN denotes %
T21492 369-375 NNP denotes Igepal
T21493 376-377 -LRB- denotes (
T21494 377-382 NNP denotes NP-40
T21495 382-383 -RRB- denotes )
T21496 383-384 , denotes ,
T21497 385-388 CD denotes 0.5
T21498 388-389 NN denotes %
T21499 390-396 NN denotes sodium
T21500 397-409 NN denotes deoxycholate
T21501 409-410 , denotes ,
T21502 411-412 CD denotes 1
T21503 413-415 NNP denotes mM
T21504 416-420 NNP denotes EDTA
T21505 420-421 , denotes ,
T21506 422-423 CD denotes 1
T21507 424-426 NNP denotes mM
T21508 427-430 NNP denotes DTT
T21509 430-431 , denotes ,
T21510 432-433 CD denotes 1
T21511 434-436 NNP denotes mM
T21512 437-441 NNP denotes PMSF
T21513 441-442 , denotes ,
T21514 443-446 CC denotes and
T21515 447-448 CD denotes 1
T21516 448-449 CD denotes ×
T21517 450-458 JJ denotes Complete
T21518 459-463 NNP denotes Mini
T21519 464-472 NNP denotes Protease
T21520 473-482 NNP denotes Inhibitor
T21521 483-484 NNP denotes [
T21522 484-489 NNP denotes Roche
T21523 489-490 NNP denotes ]
T21524 490-491 -RRB- denotes )
T21525 491-492 . denotes .
T21526 493-500 NN denotes Protein
T21527 501-514 NN denotes concentration
T21528 515-518 VBD denotes was
T21529 519-529 VBN denotes determined
T21530 530-532 IN denotes by
T21531 533-538 NNP denotes Lowry
T21532 539-544 NN denotes assay
T21533 545-546 -LRB- denotes (
T21534 546-553 NNP denotes Bio-Rad
T21535 553-554 , denotes ,
T21536 555-563 NNP denotes Hercules
T21537 563-564 , denotes ,
T21538 565-575 NNP denotes California
T21539 575-576 , denotes ,
T21540 577-583 NNP denotes United
T21541 584-590 NNPS denotes States
T21542 590-591 -RRB- denotes )
T21543 592-595 CC denotes and
T21544 596-608 JJ denotes colorimetric
T21545 609-618 NNS denotes reactions
T21546 619-623 VBD denotes were
T21547 624-628 VBN denotes read
T21548 629-634 VBG denotes using
T21549 635-636 DT denotes a
T21550 637-645 NNP denotes VersaMax
T21551 646-656 NN denotes microplate
T21552 657-663 NN denotes reader
T21553 664-665 -LRB- denotes (
T21554 665-674 JJ denotes Molecular
T21555 675-682 NNPS denotes Devices
T21556 682-683 , denotes ,
T21557 684-693 NNP denotes Sunnyvale
T21558 693-694 , denotes ,
T21559 695-705 NNP denotes California
T21560 705-706 , denotes ,
T21561 707-713 NNP denotes United
T21562 714-720 NNPS denotes States
T21563 720-721 -RRB- denotes )
T21564 722-724 IN denotes at
T21565 725-727 DT denotes an
T21566 728-738 NN denotes absorbance
T21567 739-741 IN denotes of
T21568 742-745 CD denotes 750
T21569 746-748 NN denotes nm
T21570 748-749 . denotes .
T21571 750-754 NN denotes Size
T21572 755-768 NN denotes fractionation
T21573 769-772 VBD denotes was
T21574 773-782 VBN denotes performed
T21575 783-785 IN denotes on
T21576 786-788 CD denotes 20
T21577 789-791 NN denotes μg
T21578 792-794 IN denotes of
T21579 795-809 NN denotes protein/sample
T21580 810-812 IN denotes by
T21581 813-821 NNP denotes SDS-PAGE
T21582 821-822 , denotes ,
T21583 823-826 CC denotes and
T21584 827-830 DT denotes the
T21585 831-838 NN denotes protein
T21586 839-842 VBD denotes was
T21587 843-854 VBN denotes transferred
T21588 855-857 TO denotes to
T21589 858-872 JJ denotes nitrocellulose
T21590 873-875 CC denotes or
T21591 876-880 NNP denotes PVDF
T21592 881-890 NNS denotes membranes
T21593 891-894 CC denotes and
T21594 895-904 VBN denotes subjected
T21595 905-907 TO denotes to
T21596 908-922 VBG denotes immunoblotting
T21597 923-928 VBG denotes using
T21598 929-932 DT denotes the
T21599 933-942 VBN denotes indicated
T21600 943-953 NNS denotes antibodies
T21601 953-954 . denotes .
T21602 955-960 CD denotes Foxp3
T21603 961-964 VBD denotes was
T21604 965-973 VBN denotes detected
T21605 974-979 VBG denotes using
T21606 980-986 NN denotes rabbit
T21607 987-997 JJ denotes anti-human
T21608 998-1003 JJ denotes Foxp3
T21609 1004-1014 NN denotes polyclonal
T21610 1015-1023 NN denotes antibody
T21611 1024-1025 -LRB- denotes (
T21612 1025-1031 CD denotes ab4728
T21613 1032-1034 CC denotes or
T21614 1035-1042 CD denotes ab10563
T21615 1042-1043 : denotes ;
T21616 1044-1049 NNP denotes Abcam
T21617 1049-1050 , denotes ,
T21618 1051-1060 NNP denotes Cambridge
T21619 1060-1061 , denotes ,
T21620 1062-1068 NNP denotes United
T21621 1069-1076 NNP denotes Kingdom
T21622 1076-1077 -RRB- denotes )
T21623 1078-1081 CC denotes and
T21624 1082-1093 JJ denotes anti-rabbit
T21625 1094-1101 JJ denotes IgG-HRP
T21626 1102-1111 JJ denotes secondary
T21627 1112-1120 NN denotes antibody
T21628 1121-1122 -LRB- denotes (
T21629 1122-1126 NNP denotes Cell
T21630 1127-1136 NNP denotes Signaling
T21631 1137-1147 NNP denotes Technology
T21632 1147-1148 , denotes ,
T21633 1149-1156 NNP denotes Beverly
T21634 1156-1157 , denotes ,
T21635 1158-1171 NNP denotes Massachusetts
T21636 1171-1172 , denotes ,
T21637 1173-1179 NNP denotes United
T21638 1180-1186 NNPS denotes States
T21639 1186-1187 -RRB- denotes )
T21640 1187-1188 . denotes .
T21641 1189-1194 JJ denotes NF-κB
T21642 1195-1198 NNS denotes p65
T21643 1199-1202 CC denotes and
T21644 1203-1209 NN denotes CREB-1
T21645 1210-1214 VBD denotes were
T21646 1215-1223 VBN denotes detected
T21647 1224-1229 VBG denotes using
T21648 1230-1236 NN denotes rabbit
T21649 1237-1247 JJ denotes anti-human
T21650 1248-1258 NN denotes polyclonal
T21651 1259-1260 -LRB- denotes (
T21652 1260-1263 NNS denotes p65
T21653 1263-1264 -RRB- denotes )
T21654 1265-1267 CC denotes or
T21655 1268-1278 JJ denotes monoclonal
T21656 1279-1287 NN denotes antibody
T21657 1288-1289 -LRB- denotes (
T21658 1289-1295 NN denotes CREB-1
T21659 1295-1296 : denotes ;
T21660 1297-1301 NNP denotes 48H2
T21661 1301-1302 -RRB- denotes )
T21662 1303-1304 -LRB- denotes (
T21663 1304-1308 NNP denotes Cell
T21664 1309-1318 NNP denotes Signaling
T21665 1319-1329 NNP denotes Technology
T21666 1329-1330 -RRB- denotes )
T21667 1330-1331 . denotes .
T21668 1332-1342 NN denotes Beta-actin
T21669 1343-1346 VBD denotes was
T21670 1347-1355 VBN denotes detected
T21671 1356-1361 VBG denotes using
T21672 1362-1363 DT denotes a
T21673 1364-1369 NN denotes mouse
T21674 1370-1380 NN denotes monoclonal
T21675 1381-1389 NN denotes antibody
T21676 1390-1391 -LRB- denotes (
T21677 1391-1396 NN denotes AC-15
T21678 1396-1397 : denotes ;
T21679 1398-1403 NNP denotes Sigma
T21680 1403-1404 , denotes ,
T21681 1405-1408 NNP denotes St.
T21682 1409-1414 NNP denotes Louis
T21683 1414-1415 , denotes ,
T21684 1416-1424 NNP denotes Missouri
T21685 1424-1425 , denotes ,
T21686 1426-1432 NNP denotes United
T21687 1433-1439 NNPS denotes States
T21688 1439-1440 -RRB- denotes )
T21689 1440-1441 . denotes .
T21690 1442-1445 IN denotes For
T21691 1446-1467 NN denotes coimmunoprecipitation
T21692 1468-1476 NN denotes analysis
T21693 1476-1477 , denotes ,
T21694 1478-1482 NN denotes cell
T21695 1483-1490 NNS denotes lysates
T21696 1491-1495 VBD denotes were
T21697 1496-1506 VBN denotes precleared
T21698 1507-1511 IN denotes with
T21699 1512-1514 CD denotes 30
T21700 1515-1517 NN denotes μl
T21701 1518-1520 IN denotes of
T21702 1521-1528 NN denotes protein
T21703 1529-1532 NNP denotes A/G
T21704 1533-1545 JJ denotes plus-agarose
T21705 1546-1551 NNS denotes beads
T21706 1552-1553 -LRB- denotes (
T21707 1553-1558 NNP denotes Santa
T21708 1559-1563 NNP denotes Cruz
T21709 1564-1577 NNP denotes Biotechnology
T21710 1577-1578 , denotes ,
T21711 1579-1584 NNP denotes Santa
T21712 1585-1589 NNP denotes Cruz
T21713 1589-1590 , denotes ,
T21714 1591-1601 NNP denotes California
T21715 1601-1602 , denotes ,
T21716 1603-1609 NNP denotes United
T21717 1610-1616 NNPS denotes States
T21718 1616-1617 -RRB- denotes )
T21719 1618-1621 CC denotes and
T21720 1622-1626 RB denotes then
T21721 1627-1636 VBN denotes incubated
T21722 1637-1641 IN denotes with
T21723 1642-1647 NN denotes mouse
T21724 1648-1658 NN denotes monoclonal
T21725 1659-1666 JJ denotes anti-HA
T21726 1667-1675 NN denotes antibody
T21727 1676-1677 -LRB- denotes (
T21728 1677-1680 CD denotes 6E2
T21729 1680-1681 : denotes ;
T21730 1682-1687 CD denotes 1:100
T21731 1687-1688 : denotes ;
T21732 1689-1693 NNP denotes Cell
T21733 1694-1703 NNP denotes Signaling
T21734 1704-1714 NNP denotes Technology
T21735 1714-1715 -RRB- denotes )
T21736 1716-1719 CC denotes and
T21737 1720-1722 CD denotes 30
T21738 1723-1725 NN denotes μl
T21739 1726-1728 IN denotes of
T21740 1729-1736 NN denotes protein
T21741 1737-1740 NNP denotes A/G
T21742 1741-1753 JJ denotes plus-agarose
T21743 1754-1759 NNS denotes beads
T21744 1760-1769 JJ denotes overnight
T21745 1769-1770 . denotes .
T21746 1771-1774 DT denotes The
T21747 1775-1793 NNS denotes immunoprecipitates
T21748 1794-1798 VBD denotes were
T21749 1799-1805 VBN denotes washed
T21750 1806-1810 CD denotes four
T21751 1811-1816 NNS denotes times
T21752 1817-1821 IN denotes with
T21753 1822-1826 NNP denotes RIPA
T21754 1827-1833 NN denotes buffer
T21755 1833-1834 , denotes ,
T21756 1835-1846 VBN denotes resuspended
T21757 1847-1849 IN denotes in
T21758 1850-1853 NNP denotes SDS
T21759 1854-1860 NN denotes sample
T21760 1861-1867 NN denotes buffer
T21761 1867-1868 , denotes ,
T21762 1869-1872 CC denotes and
T21763 1873-1879 VBN denotes heated
T21764 1880-1882 IN denotes at
T21765 1883-1885 CD denotes 95
T21766 1886-1887 CD denotes °
T21767 1887-1888 NNP denotes C
T21768 1889-1892 IN denotes for
T21769 1893-1894 CD denotes 5
T21770 1895-1898 NN denotes min
T21771 1898-1899 . denotes .
T21772 1900-1908 NNPS denotes Proteins
T21773 1909-1913 VBD denotes were
T21774 1914-1918 RB denotes then
T21775 1919-1926 VBN denotes treated
T21776 1927-1929 IN denotes as
T21777 1930-1939 VBN denotes described
T21778 1940-1943 IN denotes for
T21779 1944-1951 JJ denotes Western
T21780 1952-1956 NN denotes blot
T21781 1957-1965 NN denotes analysis
T21782 1965-1966 . denotes .
R16166 T21416 T21418 amod Western,analysis
R16167 T21417 T21418 compound blot,analysis
R16168 T21418 T21418 ROOT analysis,analysis
R16169 T21419 T21418 punct .,analysis
R16170 T21420 T21424 nsubjpass HEK,plated
R16171 T21421 T21422 nummod 293T,cells
R16172 T21422 T21424 nsubjpass cells,plated
R16173 T21423 T21424 auxpass were,plated
R16174 T21424 T21424 ROOT plated,plated
R16175 T21425 T21424 prep at,plated
R16176 T21426 T21427 det a,density
R16177 T21427 T21425 pobj density,at
R16178 T21428 T21427 prep of,density
R16179 T21429 T21432 nummod 5,cells/well
R16180 T21430 T21432 nummod ×,cells/well
R16181 T21431 T21432 nummod 105,cells/well
R16182 T21432 T21428 pobj cells/well,of
R16183 T21433 T21432 prep in,cells/well
R16184 T21434 T21436 amod six-well,plates
R16185 T21435 T21436 compound culture,plates
R16186 T21436 T21433 pobj plates,in
R16187 T21437 T21439 punct (,Biosciences
R16188 T21438 T21439 compound BD,Biosciences
R16189 T21439 T21436 appos Biosciences,plates
R16190 T21440 T21439 punct ),Biosciences
R16191 T21441 T21442 meta 1,d
R16192 T21442 T21442 ROOT d,d
R16193 T21443 T21446 advmod prior,with
R16194 T21444 T21443 prep to,prior
R16195 T21445 T21444 pobj transfection,to
R16196 T21446 T21442 prep with,d
R16197 T21447 T21450 det the,DNA
R16198 T21448 T21450 amod appropriate,DNA
R16199 T21449 T21450 compound plasmid,DNA
R16200 T21450 T21446 pobj DNA,with
R16201 T21451 T21450 punct (,DNA
R16202 T21452 T21453 nummod 2,μg
R16203 T21453 T21454 amod μg,total
R16204 T21454 T21450 appos total,DNA
R16205 T21455 T21450 punct ),DNA
R16206 T21456 T21442 advcl using,d
R16207 T21457 T21456 dobj FuGene,using
R16208 T21458 T21460 nummod 6,reagent
R16209 T21459 T21460 compound transfection,reagent
R16210 T21460 T21457 amod reagent,FuGene
R16211 T21461 T21460 punct (,reagent
R16212 T21462 T21460 appos Roche,reagent
R16213 T21463 T21460 punct ),reagent
R16214 T21464 T21442 punct .,d
R16215 T21465 T21467 nsubjpass Cells,harvested
R16216 T21466 T21467 auxpass were,harvested
R16217 T21467 T21467 ROOT harvested,harvested
R16218 T21468 T21470 nummod 24,posttransfection
R16219 T21469 T21470 compound h,posttransfection
R16220 T21470 T21467 dobj posttransfection,harvested
R16221 T21471 T21470 prep for,posttransfection
R16222 T21472 T21473 amod whole-cell,lysates
R16223 T21473 T21471 pobj lysates,for
R16224 T21474 T21473 prep in,lysates
R16225 T21475 T21476 compound RIPA,buffer
R16226 T21476 T21474 pobj buffer,in
R16227 T21477 T21484 punct (,]
R16228 T21478 T21484 nummod 50,]
R16229 T21479 T21482 compound mM,pH
R16230 T21480 T21482 compound Tris-HCl,pH
R16231 T21481 T21482 compound [,pH
R16232 T21482 T21484 nmod pH,]
R16233 T21483 T21484 nummod 7.4,]
R16234 T21484 T21476 appos ],buffer
R16235 T21485 T21484 punct ",",]
R16236 T21486 T21488 nummod 150,NaCl
R16237 T21487 T21488 compound mM,NaCl
R16238 T21488 T21484 appos NaCl,]
R16239 T21489 T21488 punct ",",NaCl
R16240 T21490 T21491 nummod 1,%
R16241 T21491 T21492 compound %,Igepal
R16242 T21492 T21488 conj Igepal,NaCl
R16243 T21493 T21494 punct (,NP-40
R16244 T21494 T21492 appos NP-40,Igepal
R16245 T21495 T21492 punct ),Igepal
R16246 T21496 T21492 punct ",",Igepal
R16247 T21497 T21498 nummod 0.5,%
R16248 T21498 T21500 compound %,deoxycholate
R16249 T21499 T21500 compound sodium,deoxycholate
R16250 T21500 T21492 appos deoxycholate,Igepal
R16251 T21501 T21500 punct ",",deoxycholate
R16252 T21502 T21504 nummod 1,EDTA
R16253 T21503 T21504 compound mM,EDTA
R16254 T21504 T21500 appos EDTA,deoxycholate
R16255 T21505 T21504 punct ",",EDTA
R16256 T21506 T21508 nummod 1,DTT
R16257 T21507 T21508 compound mM,DTT
R16258 T21508 T21504 appos DTT,EDTA
R16259 T21509 T21508 punct ",",DTT
R16260 T21510 T21512 nummod 1,PMSF
R16261 T21511 T21512 compound mM,PMSF
R16262 T21512 T21508 conj PMSF,DTT
R16263 T21513 T21512 punct ",",PMSF
R16264 T21514 T21512 cc and,PMSF
R16265 T21515 T21523 nummod 1,]
R16266 T21516 T21523 nummod ×,]
R16267 T21517 T21519 amod Complete,Protease
R16268 T21518 T21519 compound Mini,Protease
R16269 T21519 T21523 compound Protease,]
R16270 T21520 T21523 compound Inhibitor,]
R16271 T21521 T21523 compound [,]
R16272 T21522 T21523 compound Roche,]
R16273 T21523 T21512 conj ],PMSF
R16274 T21524 T21504 punct ),EDTA
R16275 T21525 T21467 punct .,harvested
R16276 T21526 T21527 compound Protein,concentration
R16277 T21527 T21529 nsubjpass concentration,determined
R16278 T21528 T21529 auxpass was,determined
R16279 T21529 T21529 ROOT determined,determined
R16280 T21530 T21529 agent by,determined
R16281 T21531 T21532 compound Lowry,assay
R16282 T21532 T21530 pobj assay,by
R16283 T21533 T21534 punct (,Bio-Rad
R16284 T21534 T21532 appos Bio-Rad,assay
R16285 T21535 T21534 punct ",",Bio-Rad
R16286 T21536 T21534 conj Hercules,Bio-Rad
R16287 T21537 T21536 punct ",",Hercules
R16288 T21538 T21536 conj California,Hercules
R16289 T21539 T21538 punct ",",California
R16290 T21540 T21541 compound United,States
R16291 T21541 T21538 conj States,California
R16292 T21542 T21541 punct ),States
R16293 T21543 T21541 cc and,States
R16294 T21544 T21541 conj colorimetric,States
R16295 T21545 T21547 nsubjpass reactions,read
R16296 T21546 T21547 auxpass were,read
R16297 T21547 T21529 conj read,determined
R16298 T21548 T21547 advcl using,read
R16299 T21549 T21552 det a,reader
R16300 T21550 T21552 nmod VersaMax,reader
R16301 T21551 T21552 compound microplate,reader
R16302 T21552 T21548 dobj reader,using
R16303 T21553 T21552 punct (,reader
R16304 T21554 T21555 compound Molecular,Devices
R16305 T21555 T21552 appos Devices,reader
R16306 T21556 T21555 punct ",",Devices
R16307 T21557 T21555 conj Sunnyvale,Devices
R16308 T21558 T21557 punct ",",Sunnyvale
R16309 T21559 T21557 conj California,Sunnyvale
R16310 T21560 T21559 punct ",",California
R16348 T21598 T21600 det the,antibodies
R16349 T21599 T21600 amod indicated,antibodies
R16350 T21600 T21597 dobj antibodies,using
R16351 T21601 T21587 punct .,transferred
R16352 T21602 T21604 nsubjpass Foxp3,detected
R16353 T21603 T21604 auxpass was,detected
R16354 T21604 T21604 ROOT detected,detected
R16355 T21605 T21604 advcl using,detected
R16356 T21606 T21610 nmod rabbit,antibody
R16357 T21607 T21610 nmod anti-human,antibody
R16358 T21608 T21610 amod Foxp3,antibody
R16359 T21609 T21610 compound polyclonal,antibody
R16360 T21610 T21605 dobj antibody,using
R16361 T21611 T21610 punct (,antibody
R16362 T21612 T21610 appos ab4728,antibody
R16363 T21613 T21612 cc or,ab4728
R16364 T21614 T21612 conj ab10563,ab4728
R16365 T21615 T21610 punct ;,antibody
R16366 T21616 T21627 nmod Abcam,antibody
R16367 T21617 T21616 punct ",",Abcam
R16368 T21618 T21616 conj Cambridge,Abcam
R16369 T21619 T21618 punct ",",Cambridge
R16370 T21620 T21621 compound United,Kingdom
R16371 T21621 T21618 appos Kingdom,Cambridge
R16372 T21622 T21618 punct ),Cambridge
R16373 T21623 T21618 cc and,Cambridge
R16374 T21624 T21627 nmod anti-rabbit,antibody
R16375 T21625 T21627 amod IgG-HRP,antibody
R16376 T21626 T21627 amod secondary,antibody
R16377 T21627 T21610 appos antibody,antibody
R16378 T21628 T21631 punct (,Technology
R16379 T21629 T21631 compound Cell,Technology
R16380 T21630 T21631 compound Signaling,Technology
R16381 T21631 T21627 appos Technology,antibody
R16382 T21632 T21631 punct ",",Technology
R16383 T21633 T21631 conj Beverly,Technology
R16384 T21634 T21633 punct ",",Beverly
R16385 T21635 T21633 conj Massachusetts,Beverly
R16386 T21636 T21635 punct ",",Massachusetts
R16387 T21637 T21638 compound United,States
R16388 T21638 T21635 conj States,Massachusetts
R16389 T21639 T21635 punct ),Massachusetts
R16390 T21640 T21604 punct .,detected
R16391 T21641 T21642 amod NF-κB,p65
R16392 T21642 T21646 nsubjpass p65,detected
R16393 T21643 T21642 cc and,p65
R16394 T21644 T21642 conj CREB-1,p65
R16395 T21645 T21646 auxpass were,detected
R16396 T21646 T21660 ccomp detected,48H2
R16397 T21647 T21646 advcl using,detected
R16398 T21648 T21650 nmod rabbit,polyclonal
R16399 T21649 T21650 amod anti-human,polyclonal
R16400 T21650 T21647 dobj polyclonal,using
R16401 T21651 T21652 punct (,p65
R16402 T21652 T21658 nmod p65,CREB-1
R16403 T21653 T21652 punct ),p65
R16404 T21654 T21652 cc or,p65
R16405 T21655 T21656 amod monoclonal,antibody
R16406 T21656 T21652 conj antibody,p65
R16407 T21657 T21658 punct (,CREB-1
R16408 T21658 T21650 appos CREB-1,polyclonal
R16409 T21659 T21660 punct ;,48H2
R16410 T21660 T21660 ROOT 48H2,48H2
R16411 T21661 T21660 punct ),48H2
R16412 T21662 T21664 punct (,Signaling
R16413 T21663 T21664 compound Cell,Signaling
R16414 T21664 T21660 appos Signaling,48H2
R16415 T21665 T21664 pobj Technology,Signaling
R16416 T21666 T21664 punct ),Signaling
R16417 T21667 T21660 punct .,48H2
R16418 T21668 T21670 nsubjpass Beta-actin,detected
R16419 T21669 T21670 auxpass was,detected
R16420 T21670 T21670 ROOT detected,detected
R16421 T21671 T21670 advcl using,detected
R16422 T21672 T21675 det a,antibody
R16423 T21673 T21675 compound mouse,antibody
R16424 T21674 T21675 compound monoclonal,antibody
R16425 T21675 T21671 dobj antibody,using
R16426 T21676 T21675 punct (,antibody
R16427 T21677 T21675 appos AC-15,antibody
R16428 T21678 T21675 punct ;,antibody
R16429 T21679 T21675 conj Sigma,antibody
R16430 T21680 T21679 punct ",",Sigma
R16431 T21681 T21682 compound St.,Louis
R16432 T21682 T21679 conj Louis,Sigma
R16433 T21683 T21682 punct ",",Louis
R16434 T21684 T21682 conj Missouri,Louis
R16435 T21685 T21684 punct ",",Missouri
R16436 T21686 T21687 compound United,States
R16437 T21687 T21684 appos States,Missouri
R16438 T21688 T21684 punct ),Missouri
R16439 T21689 T21670 punct .,detected
R16440 T21690 T21697 prep For,precleared
R16441 T21691 T21692 compound coimmunoprecipitation,analysis
R16442 T21692 T21690 pobj analysis,For
R16443 T21693 T21697 punct ",",precleared
R16444 T21694 T21695 compound cell,lysates
R16445 T21695 T21697 nsubjpass lysates,precleared
R16446 T21696 T21697 auxpass were,precleared
R16447 T21697 T21697 ROOT precleared,precleared
R16448 T21698 T21697 prep with,precleared
R16449 T21699 T21700 nummod 30,μl
R16450 T21700 T21698 pobj μl,with
R16451 T21701 T21700 prep of,μl
R16452 T21702 T21705 nmod protein,beads
R16453 T21703 T21705 nmod A/G,beads
R16454 T21704 T21705 amod plus-agarose,beads
R16455 T21705 T21701 pobj beads,of
R16456 T21706 T21709 punct (,Biotechnology
R16457 T21707 T21708 compound Santa,Cruz
R16458 T21708 T21709 compound Cruz,Biotechnology
R16459 T21709 T21705 appos Biotechnology,beads
R16460 T21710 T21709 punct ",",Biotechnology
R16461 T21711 T21712 compound Santa,Cruz
R16462 T21712 T21709 conj Cruz,Biotechnology
R16463 T21713 T21712 punct ",",Cruz
R16464 T21714 T21712 conj California,Cruz
R16465 T21715 T21714 punct ",",California
R16466 T21716 T21717 compound United,States
R16467 T21717 T21714 conj States,California
R16468 T21718 T21717 punct ),States
R16469 T21719 T21697 cc and,precleared
R16470 T21720 T21721 advmod then,incubated
R16471 T21721 T21697 conj incubated,precleared
R16472 T21722 T21721 prep with,incubated
R16473 T21723 T21726 nmod mouse,antibody
R16474 T21724 T21726 nmod monoclonal,antibody
R16475 T21725 T21726 amod anti-HA,antibody
R16476 T21726 T21722 pobj antibody,with
R16477 T21727 T21726 punct (,antibody
R16478 T21728 T21726 appos 6E2,antibody
R16479 T21729 T21726 punct ;,antibody
R16480 T21730 T21726 conj 1:100,antibody
R16481 T21731 T21734 punct ;,Technology
R16482 T21732 T21734 compound Cell,Technology
R16483 T21733 T21734 compound Signaling,Technology
R16484 T21734 T21697 conj Technology,precleared
R16485 T21735 T21734 punct ),Technology
R16486 T21736 T21734 cc and,Technology
R16487 T21737 T21738 nummod 30,μl
R16488 T21738 T21744 npadvmod μl,overnight
R16489 T21739 T21738 prep of,μl
R16490 T21740 T21743 nmod protein,beads
R16491 T21741 T21743 nmod A/G,beads
R16492 T21742 T21743 amod plus-agarose,beads
R16493 T21743 T21739 pobj beads,of
R16494 T21744 T21734 conj overnight,Technology
R16495 T21745 T21697 punct .,precleared
R16496 T21746 T21747 det The,immunoprecipitates
R16497 T21747 T21749 nsubjpass immunoprecipitates,washed
R16498 T21748 T21749 auxpass were,washed
R16499 T21749 T21749 ROOT washed,washed
R16500 T21750 T21751 nummod four,times
R16501 T21751 T21749 npadvmod times,washed
R16502 T21752 T21749 prep with,washed
R16503 T21753 T21754 compound RIPA,buffer
R16504 T21754 T21752 pobj buffer,with
R16505 T21755 T21754 punct ",",buffer
R16506 T21756 T21749 conj resuspended,washed
R16507 T21757 T21756 prep in,resuspended
R16508 T21758 T21760 compound SDS,buffer
R16509 T21759 T21760 compound sample,buffer
R16510 T21760 T21757 pobj buffer,in
R16511 T21761 T21756 punct ",",resuspended
R16512 T21762 T21756 cc and,resuspended
R16513 T21763 T21756 conj heated,resuspended
R16514 T21764 T21763 prep at,heated
R16515 T21765 T21766 nummod 95,°
R16516 T21766 T21764 pobj °,at
R16517 T21767 T21763 conj C,heated
R16518 T21768 T21767 prep for,C
R16519 T21769 T21770 nummod 5,min
R16520 T21770 T21768 pobj min,for
R16521 T21771 T21749 punct .,washed
R16522 T21772 T21775 nsubjpass Proteins,treated
R16523 T21773 T21775 auxpass were,treated
R16524 T21774 T21775 advmod then,treated
R16525 T21775 T21775 ROOT treated,treated
R16526 T21776 T21777 mark as,described
R16527 T21777 T21775 advcl described,treated
R16528 T21778 T21777 prep for,described
R16529 T21779 T21781 amod Western,analysis
R16530 T21780 T21781 compound blot,analysis
R16531 T21781 T21778 pobj analysis,for
R16532 T21782 T21775 punct .,treated
R16311 T21561 T21562 compound United,States
R16312 T21562 T21559 conj States,California
R16313 T21563 T21559 punct ),California
R16314 T21564 T21548 prep at,using
R16315 T21565 T21566 det an,absorbance
R16316 T21566 T21564 pobj absorbance,at
R16317 T21567 T21566 prep of,absorbance
R16318 T21568 T21569 nummod 750,nm
R16319 T21569 T21567 pobj nm,of
R16320 T21570 T21547 punct .,read
R16321 T21571 T21572 compound Size,fractionation
R16322 T21572 T21574 nsubjpass fractionation,performed
R16323 T21573 T21574 auxpass was,performed
R16324 T21574 T21574 ROOT performed,performed
R16325 T21575 T21574 prep on,performed
R16326 T21576 T21577 nummod 20,μg
R16327 T21577 T21575 pobj μg,on
R16328 T21578 T21577 prep of,μg
R16329 T21579 T21578 pobj protein/sample,of
R16330 T21580 T21577 prep by,μg
R16331 T21581 T21580 pobj SDS-PAGE,by
R16332 T21582 T21574 punct ",",performed
R16333 T21583 T21574 cc and,performed
R16334 T21584 T21585 det the,protein
R16335 T21585 T21587 nsubjpass protein,transferred
R16336 T21586 T21587 auxpass was,transferred
R16337 T21587 T21574 conj transferred,performed
R16338 T21588 T21587 prep to,transferred
R16339 T21589 T21592 amod nitrocellulose,membranes
R16340 T21590 T21589 cc or,nitrocellulose
R16341 T21591 T21589 conj PVDF,nitrocellulose
R16342 T21592 T21588 pobj membranes,to
R16343 T21593 T21587 cc and,transferred
R16344 T21594 T21587 conj subjected,transferred
R16345 T21595 T21594 prep to,subjected
R16346 T21596 T21595 pcomp immunoblotting,to
R16347 T21597 T21594 advcl using,subjected

GO-MF

Id Subject Object Predicate Lexical cue
T22172 943-953 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T22173 1015-1023 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T22174 1112-1120 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T22175 1279-1287 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T22176 1381-1389 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T22177 1667-1675 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody

GO-CC

Id Subject Object Predicate Lexical cue
T22178 32-37 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T22179 74-79 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T22180 299-303 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T22181 1478-1482 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T22182 881-890 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T22183 943-953 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T22184 1015-1023 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T22185 1112-1120 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T22186 1279-1287 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T22187 1381-1389 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T22188 1667-1675 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T22189 943-953 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T22190 1015-1023 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T22191 1112-1120 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T22192 1279-1287 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T22193 1381-1389 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T22194 1667-1675 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody

events-check-again

Id Subject Object Predicate Lexical cue
T22230 955-960 Protein denotes Foxp3
T22231 998-1003 Protein denotes Foxp3
T22232 1195-1198 Protein denotes p65
T22233 1203-1209 Protein denotes CREB-1
T22234 1260-1263 Protein denotes p65
T22235 1289-1295 Protein denotes CREB-1
T22236 1332-1342 Protein denotes Beta-actin

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T21398 955-960 Protein denotes Foxp3
T21399 998-1003 Protein denotes Foxp3
T21400 1195-1198 Protein denotes p65
T21401 1203-1209 Protein denotes CREB-1
T21402 1260-1263 Protein denotes p65
T21403 1289-1295 Protein denotes CREB-1
T21404 1332-1342 Protein denotes Beta-actin

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T22139 344-346 P0A7Z4 denotes pH
T22140 955-960 Q9BZS1 denotes Foxp3
T22141 998-1003 Q9BZS1 denotes Foxp3
T22142 1195-1198 P21579 denotes p65
T22143 1195-1198 Q04206 denotes p65
T22144 1203-1207 P15336 denotes CREB
T22145 1203-1207 O43889 denotes CREB
T22146 1203-1207 Q8TEY5 denotes CREB
T22147 1203-1207 Q02930 denotes CREB
T22148 1203-1207 P18848 denotes CREB
T22149 1203-1207 P16220 denotes CREB
T22150 1203-1209 P16220 denotes CREB-1
T22151 1260-1263 P21579 denotes p65
T22152 1260-1263 Q04206 denotes p65
T22153 1289-1293 P15336 denotes CREB
T22154 1289-1293 P16220 denotes CREB
T22155 1289-1293 Q02930 denotes CREB
T22156 1289-1293 Q8TEY5 denotes CREB
T22157 1289-1293 P18848 denotes CREB
T22158 1289-1293 O43889 denotes CREB
T22159 1289-1295 P16220 denotes CREB-1
T22160 1332-1342 P60709 denotes Beta-actin

test2

Id Subject Object Predicate Lexical cue
T21391 955-960 Protein denotes Foxp3
T21392 998-1003 Protein denotes Foxp3
T21393 1195-1198 Protein denotes p65
T21394 1203-1209 Protein denotes CREB-1
T21395 1260-1263 Protein denotes p65
T21396 1289-1295 Protein denotes CREB-1
T21397 1332-1342 Protein denotes Beta-actin