| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T90 |
0-58 |
Sentence |
denotes |
Figure 3 Foxp3 Inhibits Basal Activation of the HIV-1 LTR |
| T91 |
59-442 |
Sentence |
denotes |
(A and B) HEK 293T cells (5 × 105) (A) were seeded into six-well plates 1 d prior to transfection or Jurkat T cells (1 × 106) (B) were seeded into six-well plates on the day of transfection with an HIV-1 LTR luciferase reporter vector (200 ng) in the presence or absence of a Foxp3, ΔFKH, or control expression vector (1,000 ng) and internal reference plasmid (pGL4-TKhRluc2; 50 ng). |
| T92 |
443-526 |
Sentence |
denotes |
Cells were harvested at 24 h posttransfection and analyzed for luciferase activity. |
| T93 |
527-639 |
Sentence |
denotes |
(C) Schematic representation of HIV-1 LTR luciferase reporter constructs pHIV-1 LTR-luc and pHIV-1 Δ-κB LTR-luc. |
| T94 |
640-917 |
Sentence |
denotes |
(D and E) CD4+ T cells (2 × 106) from two healthy donors were nucleofected with either parental HIV-1 LTR (D) or HIV-1 LTR lacking NF-κB binding sites (at −102 to −81) (E) (1,000 ng), Foxp3 or control expression vectors (2,000 ng), and an internal reference plasmid (1,000 ng). |
| T95 |
918-998 |
Sentence |
denotes |
Cells were harvested 24 h posttransfection and analyzed for luciferase activity. |
| T96 |
999-1101 |
Sentence |
denotes |
Relative luciferase activity shown in A, B, D, and E was normalized to the internal reference control. |
