PMC:1440874 / 57695-58664 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1440874","sourcedb":"PMC","sourceid":"1440874","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1440874","text":"Normal DNA Methylation at Mouse Repetitive Elements in Atrxnull ES Cells\nDNA from Atrx-positive (bearing either an Atrx WT or Atrx flox allele) or Atrxnull (bearing the Atrx Δ18Δneo allele) ES cells was digested with either a CpG-methylation-sensitive enzyme HpaII (H) or its methylation-insensitive isoschizomer MspI (M) as indicated, and digested DNA was analysed by Southern blotting. Membranes were hybridised with probes specific for the mouse Line 1 (A), Sine B1 (B), minor satellite (C), and IAP (D) repeat elements. No significant loss of CpG-methylation was observed at any of these repetitive elements in Atrxnull ES cells. As a positive control for loss of methylation, DNA from ES cells lacking either the Dnmt1 (Dnmt1−/−) or both the Dnmt3a and Dnmt3b (Dnmt3a3b−/−) DNA methyltransferases was included in the analysis of Line 1 and Sine B1 repeats. Loss of CpG-methylation was clearly observed at these repetitive elements in these cell lines.\n(1.8 MB PDF)","divisions":[{"label":"title","span":{"begin":0,"end":72}},{"label":"p","span":{"begin":73,"end":956}}],"tracks":[]}