PMC:1440874 / 56156-57184 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1440874","sourcedb":"PMC","sourceid":"1440874","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1440874","text":"Cell Cycle Analysis of Atrx-Positive and Atrxnull ES Cells\n(A) Representative FACS profiles of BrdU-pulsed ES cells bearing either functional (Atrx WT or Atrx flox) or null (Atrx Δ18Δneo) Atrx alleles showing BrdU-FITC (y-axis, logarithmic scale) against propidium iodide (x-axis, linear scale). The gated populations show cells in G1 (PIlow, BrdU-negative) (R1), S (BrdU-positive) (R2), and G2/M (PIhi, BrdU-negative) (R3) phases of the cell cycle. Below is shown the quantitation of gated populations, indicating the percentage of cells in G1, S, and G2/M cell-cycle phases in each culture.\n(B) FACS profiles of ES cells bearing either functional (Atrx WT or Atrx flox) or null (Atrx Δ18Δneo) Atrx alleles stained for the mitosis marker phosphorylated (Ser10) histone H3 (FITC, y-axis, logarithmic scale) against propidium iodide (x-axis, linear scale). The size of the mitotic population (phosphoH3S10-positive, PIhi)(gate R3) is indicated for each profile. The ES cell clone analysed in each trace is indicated.\n(3.2 MB PDF)","divisions":[{"label":"title","span":{"begin":0,"end":58}},{"label":"p","span":{"begin":59,"end":592}},{"label":"p","span":{"begin":593,"end":1015}}],"tracks":[]}