PMC:1440874 / 53393-53999 JSONTXT

{"project":"craft-sa-dev","denotations":[{"id":"T12092","span":{"begin":0,"end":8},"obj":"VBN"},{"id":"T12093","span":{"begin":27,"end":33},"obj":"NNS"},{"id":"T12094","span":{"begin":9,"end":18},"obj":"NN"},{"id":"T12095","span":{"begin":22,"end":26},"obj":"NN"},{"id":"T12096","span":{"begin":19,"end":21},"obj":"NN"},{"id":"T12097","span":{"begin":39,"end":47},"obj":"VBN"},{"id":"T12098","span":{"begin":34,"end":38},"obj":"VBD"},{"id":"T12099","span":{"begin":48,"end":52},"obj":"IN"},{"id":"T12100","span":{"begin":53,"end":58},"obj":"NN"},{"id":"T12101","span":{"begin":61,"end":72},"obj":"NNS"},{"id":"T12102","span":{"begin":58,"end":59},"obj":"HYPH"},{"id":"T12103","span":{"begin":59,"end":60},"obj":"CD"},{"id":"T12104","span":{"begin":73,"end":76},"obj":"CC"},{"id":"T12105","span":{"begin":77,"end":88},"obj":"VBN"},{"id":"T12106","span":{"begin":89,"end":93},"obj":"IN"},{"id":"T12107","span":{"begin":94,"end":97},"obj":"CD"},{"id":"T12108","span":{"begin":98,"end":101},"obj":"NN"},{"id":"T12109","span":{"begin":117,"end":127},"obj":"NNS"},{"id":"T12110","span":{"begin":102,"end":116},"obj":"JJ"},{"id":"T12111","span":{"begin":128,"end":130},"obj":"IN"},{"id":"T12112","span":{"begin":131,"end":139},"obj":"JJ"},{"id":"T12113","span":{"begin":140,"end":150},"obj":"NNS"},{"id":"T12114","span":{"begin":150,"end":151},"obj":"."},{"id":"T12115","span":{"begin":151,"end":230},"obj":"sentence"},{"id":"T12116","span":{"begin":152,"end":161},"obj":"VBG"},{"id":"T12117","span":{"begin":162,"end":170},"obj":"NNS"},{"id":"T12118","span":{"begin":176,"end":181},"obj":"VBN"},{"id":"T12119","span":{"begin":171,"end":175},"obj":"VBD"},{"id":"T12120","span":{"begin":182,"end":186},"obj":"IN"},{"id":"T12121","span":{"begin":187,"end":192},"obj":"NN"},{"id":"T12122","span":{"begin":192,"end":193},"obj":"HYPH"},{"id":"T12123","span":{"begin":193,"end":194},"obj":"CD"},{"id":"T12124","span":{"begin":195,"end":197},"obj":"TO"},{"id":"T12125","span":{"begin":198,"end":207},"obj":"VB"},{"id":"T12126","span":{"begin":208,"end":216},"obj":"NN"},{"id":"T12127","span":{"begin":217,"end":229},"obj":"NN"},{"id":"T12128","span":{"begin":229,"end":230},"obj":"."},{"id":"T12129","span":{"begin":230,"end":385},"obj":"sentence"},{"id":"T12130","span":{"begin":231,"end":240},"obj":"NN"},{"id":"T12131","span":{"begin":272,"end":282},"obj":"VBN"},{"id":"T12132","span":{"begin":241,"end":245},"obj":"IN"},{"id":"T12133","span":{"begin":246,"end":249},"obj":"DT"},{"id":"T12134","span":{"begin":260,"end":266},"obj":"NN"},{"id":"T12135","span":{"begin":250,"end":259},"obj":"NN"},{"id":"T12136","span":{"begin":267,"end":271},"obj":"VBD"},{"id":"T12137","span":{"begin":283,"end":285},"obj":"IN"},{"id":"T12138","span":{"begin":286,"end":294},"obj":"NNP"},{"id":"T12139","span":{"begin":295,"end":303},"obj":"NN"},{"id":"T12140","span":{"begin":304,"end":306},"obj":"IN"},{"id":"T12141","span":{"begin":307,"end":311},"obj":"NN"},{"id":"T12142","span":{"begin":312,"end":320},"obj":"VBN"},{"id":"T12143","span":{"begin":311,"end":312},"obj":"HYPH"},{"id":"T12144","span":{"begin":326,"end":329},"obj":"NN"},{"id":"T12145","span":{"begin":321,"end":325},"obj":"NN"},{"id":"T12146","span":{"begin":330,"end":335},"obj":"VBG"},{"id":"T12147","span":{"begin":336,"end":339},"obj":"DT"},{"id":"T12148","span":{"begin":350,"end":355},"obj":"NN"},{"id":"T12149","span":{"begin":340,"end":346},"obj":"NN"},{"id":"T12150","span":{"begin":347,"end":349},"obj":"CD"},{"id":"T12151","span":{"begin":356,"end":358},"obj":"IN"},{"id":"T12152","span":{"begin":359,"end":364},"obj":"VBN"},{"id":"T12153","span":{"begin":365,"end":367},"obj":"IN"},{"id":"T12154","span":{"begin":368,"end":374},"obj":"NN"},{"id":"T12155","span":{"begin":375,"end":377},"obj":"NN"},{"id":"T12156","span":{"begin":378,"end":381},"obj":"CC"},{"id":"T12157","span":{"begin":382,"end":384},"obj":"NN"},{"id":"T12158","span":{"begin":384,"end":385},"obj":"."},{"id":"T12159","span":{"begin":385,"end":493},"obj":"sentence"},{"id":"T12160","span":{"begin":386,"end":389},"obj":"IN"},{"id":"T12161","span":{"begin":429,"end":436},"obj":"VBN"},{"id":"T12162","span":{"begin":390,"end":393},"obj":"NN"},{"id":"T12163","span":{"begin":394,"end":407},"obj":"NN"},{"id":"T12164","span":{"begin":393,"end":394},"obj":"HYPH"},{"id":"T12165","span":{"begin":407,"end":409},"obj":","},{"id":"T12166","span":{"begin":409,"end":418},"obj":"NN"},{"id":"T12167","span":{"begin":419,"end":423},"obj":"NNS"},{"id":"T12168","span":{"begin":424,"end":428},"obj":"VBD"},{"id":"T12169","span":{"begin":437,"end":441},"obj":"IN"},{"id":"T12170","span":{"begin":442,"end":447},"obj":"NN"},{"id":"T12171","span":{"begin":448,"end":451},"obj":"NN"},{"id":"T12172","span":{"begin":447,"end":448},"obj":"HYPH"},{"id":"T12173","span":{"begin":452,"end":462},"obj":"JJ"},{"id":"T12174","span":{"begin":463,"end":467},"obj":"NNS"},{"id":"T12175","span":{"begin":468,"end":470},"obj":"IN"},{"id":"T12176","span":{"begin":471,"end":480},"obj":"VBN"},{"id":"T12177","span":{"begin":481,"end":483},"obj":"IN"},{"id":"T12178","span":{"begin":484,"end":487},"obj":"DT"},{"id":"T12179","span":{"begin":488,"end":492},"obj":"NN"},{"id":"T12180","span":{"begin":492,"end":493},"obj":"."},{"id":"T12181","span":{"begin":493,"end":606},"obj":"sentence"},{"id":"T12182","span":{"begin":494,"end":505},"obj":"JJ"},{"id":"T12183","span":{"begin":506,"end":513},"obj":"NNS"},{"id":"T12184","span":{"begin":519,"end":527},"obj":"VBN"},{"id":"T12185","span":{"begin":514,"end":518},"obj":"VBD"},{"id":"T12186","span":{"begin":528,"end":530},"obj":"IN"},{"id":"T12187","span":{"begin":531,"end":539},"obj":"NNP"},{"id":"T12188","span":{"begin":540,"end":548},"obj":"NN"},{"id":"T12189","span":{"begin":549,"end":551},"obj":"IN"},{"id":"T12190","span":{"begin":552,"end":561},"obj":"VBN"},{"id":"T12191","span":{"begin":562,"end":567},"obj":"RB"},{"id":"T12192","span":{"begin":568,"end":570},"obj":"CC"},{"id":"T12193","span":{"begin":571,"end":573},"obj":"IN"},{"id":"T12194","span":{"begin":574,"end":577},"obj":"NN"},{"id":"T12195","span":{"begin":578,"end":580},"obj":"IN"},{"id":"T12196","span":{"begin":581,"end":590},"obj":"VBN"},{"id":"T12197","span":{"begin":591,"end":593},"obj":"IN"},{"id":"T12198","span":{"begin":594,"end":602},"obj":"NN"},{"id":"T12199","span":{"begin":603,"end":605},"obj":"NN"},{"id":"T12200","span":{"begin":605,"end":606},"obj":"."}],"relations":[{"id":"R7562","pred":"amod","subj":"T12092","obj":"T12093"},{"id":"R7563","pred":"nsubjpass","subj":"T12093","obj":"T12097"},{"id":"R7564","pred":"compound","subj":"T12094","obj":"T12095"},{"id":"R7565","pred":"compound","subj":"T12095","obj":"T12093"},{"id":"R7566","pred":"compound","subj":"T12096","obj":"T12095"},{"id":"R7567","pred":"auxpass","subj":"T12098","obj":"T12097"},{"id":"R7568","pred":"prep","subj":"T12099","obj":"T12097"},{"id":"R7569","pred":"nmod","subj":"T12100","obj":"T12101"},{"id":"R7570","pred":"pobj","subj":"T12101","obj":"T12099"},{"id":"R7571","pred":"punct","subj":"T12102","obj":"T12100"},{"id":"R7572","pred":"nummod","subj":"T12103","obj":"T12100"},{"id":"R7573","pred":"cc","subj":"T12104","obj":"T12097"},{"id":"R7574","pred":"conj","subj":"T12105","obj":"T12097"},{"id":"R7575","pred":"prep","subj":"T12106","obj":"T12105"},{"id":"R7576","pred":"nummod","subj":"T12107","obj":"T12108"},{"id":"R7577","pred":"nmod","subj":"T12108","obj":"T12109"},{"id":"R7578","pred":"pobj","subj":"T12109","obj":"T12106"},{"id":"R7579","pred":"amod","subj":"T12110","obj":"T12109"},{"id":"R7580","pred":"prep","subj":"T12111","obj":"T12097"},{"id":"R7581","pred":"amod","subj":"T12112","obj":"T12113"},{"id":"R7582","pred":"pobj","subj":"T12113","obj":"T12111"},{"id":"R7583","pred":"punct","subj":"T12114","obj":"T12097"},{"id":"R7584","pred":"amod","subj":"T12116","obj":"T12117"},{"id":"R7585","pred":"nsubjpass","subj":"T12117","obj":"T12118"},{"id":"R7586","pred":"auxpass","subj":"T12119","obj":"T12118"},{"id":"R7587","pred":"prep","subj":"T12120","obj":"T12118"},{"id":"R7588","pred":"pobj","subj":"T12121","obj":"T12120"},{"id":"R7589","pred":"punct","subj":"T12122","obj":"T12121"},{"id":"R7590","pred":"nummod","subj":"T12123","obj":"T12121"},{"id":"R7591","pred":"aux","subj":"T12124","obj":"T12125"},{"id":"R7592","pred":"advcl","subj":"T12125","obj":"T12118"},{"id":"R7593","pred":"compound","subj":"T12126","obj":"T12127"},{"id":"R7594","pred":"dobj","subj":"T12127","obj":"T12125"},{"id":"R7595","pred":"punct","subj":"T12128","obj":"T12118"},{"id":"R7596","pred":"nsubjpass","subj":"T12130","obj":"T12131"},{"id":"R7597","pred":"prep","subj":"T12132","obj":"T12130"},{"id":"R7598","pred":"det","subj":"T12133","obj":"T12134"},{"id":"R7599","pred":"pobj","subj":"T12134","obj":"T12132"},{"id":"R7600","pred":"compound","subj":"T12135","obj":"T12134"},{"id":"R7601","pred":"auxpass","subj":"T12136","obj":"T12131"},{"id":"R7602","pred":"prep","subj":"T12137","obj":"T12131"},{"id":"R7603","pred":"compound","subj":"T12138","obj":"T12139"},{"id":"R7604","pred":"pobj","subj":"T12139","obj":"T12137"},{"id":"R7605","pred":"prep","subj":"T12140","obj":"T12139"},{"id":"R7606","pred":"npadvmod","subj":"T12141","obj":"T12142"},{"id":"R7607","pred":"amod","subj":"T12142","obj":"T12144"},{"id":"R7608","pred":"punct","subj":"T12143","obj":"T12142"},{"id":"R7609","pred":"pobj","subj":"T12144","obj":"T12140"},{"id":"R7610","pred":"compound","subj":"T12145","obj":"T12144"},{"id":"R7611","pred":"advcl","subj":"T12146","obj":"T12131"},{"id":"R7612","pred":"det","subj":"T12147","obj":"T12148"},{"id":"R7613","pred":"dobj","subj":"T12148","obj":"T12146"},{"id":"R7614","pred":"nmod","subj":"T12149","obj":"T12148"},{"id":"R7615","pred":"nummod","subj":"T12150","obj":"T12149"},{"id":"R7616","pred":"mark","subj":"T12151","obj":"T12152"},{"id":"R7617","pred":"advcl","subj":"T12152","obj":"T12146"},{"id":"R7618","pred":"prep","subj":"T12153","obj":"T12152"},{"id":"R7619","pred":"compound","subj":"T12154","obj":"T12155"},{"id":"R7620","pred":"pobj","subj":"T12155","obj":"T12153"},{"id":"R7621","pred":"cc","subj":"T12156","obj":"T12155"},{"id":"R7622","pred":"conj","subj":"T12157","obj":"T12155"},{"id":"R7623","pred":"punct","subj":"T12158","obj":"T12131"},{"id":"R7624","pred":"prep","subj":"T12160","obj":"T12161"},{"id":"R7625","pred":"compound","subj":"T12162","obj":"T12163"},{"id":"R7626","pred":"pobj","subj":"T12163","obj":"T12160"},{"id":"R7627","pred":"punct","subj":"T12164","obj":"T12163"},{"id":"R7628","pred":"punct","subj":"T12165","obj":"T12161"},{"id":"R7629","pred":"compound","subj":"T12166","obj":"T12167"},{"id":"R7630","pred":"nsubjpass","subj":"T12167","obj":"T12161"},{"id":"R7631","pred":"auxpass","subj":"T12168","obj":"T12161"},{"id":"R7632","pred":"prep","subj":"T12169","obj":"T12161"},{"id":"R7633","pred":"compound","subj":"T12170","obj":"T12171"},{"id":"R7634","pred":"npadvmod","subj":"T12171","obj":"T12173"},{"id":"R7635","pred":"punct","subj":"T12172","obj":"T12171"},{"id":"R7636","pred":"amod","subj":"T12173","obj":"T12174"},{"id":"R7637","pred":"pobj","subj":"T12174","obj":"T12169"},{"id":"R7638","pred":"mark","subj":"T12175","obj":"T12176"},{"id":"R7639","pred":"advcl","subj":"T12176","obj":"T12161"},{"id":"R7640","pred":"prep","subj":"T12177","obj":"T12176"},{"id":"R7641","pred":"det","subj":"T12178","obj":"T12179"},{"id":"R7642","pred":"pobj","subj":"T12179","obj":"T12177"},{"id":"R7643","pred":"punct","subj":"T12180","obj":"T12161"},{"id":"R7644","pred":"amod","subj":"T12182","obj":"T12183"},{"id":"R7645","pred":"nsubjpass","subj":"T12183","obj":"T12184"},{"id":"R7646","pred":"auxpass","subj":"T12185","obj":"T12184"},{"id":"R7647","pred":"prep","subj":"T12186","obj":"T12184"},{"id":"R7648","pred":"compound","subj":"T12187","obj":"T12188"},{"id":"R7649","pred":"pobj","subj":"T12188","obj":"T12186"},{"id":"R7650","pred":"mark","subj":"T12189","obj":"T12190"},{"id":"R7651","pred":"advcl","subj":"T12190","obj":"T12188"},{"id":"R7652","pred":"advmod","subj":"T12191","obj":"T12190"},{"id":"R7653","pred":"cc","subj":"T12192","obj":"T12186"},{"id":"R7654","pred":"conj","subj":"T12193","obj":"T12186"},{"id":"R7655","pred":"pobj","subj":"T12194","obj":"T12193"},{"id":"R7656","pred":"mark","subj":"T12195","obj":"T12196"},{"id":"R7657","pred":"advcl","subj":"T12196","obj":"T12184"},{"id":"R7658","pred":"prep","subj":"T12197","obj":"T12196"},{"id":"R7659","pred":"compound","subj":"T12198","obj":"T12199"},{"id":"R7660","pred":"pobj","subj":"T12199","obj":"T12197"},{"id":"R7661","pred":"punct","subj":"T12200","obj":"T12184"}],"text":"Targeted Atrx flox ES cell clones were injected into C57BL/6 blastocysts and transferred into 2.5 dpc pseudopregnant recipients by standard techniques. Resulting chimeras were mated with C57BL/6 to establish germline transmission. Offspring with the Atrx flox allele were identified by Southern blotting of SacI-digested tail DNA using the intron 17 probe as shown in Figure 2A and 2C. For Cre-recombination, Atrx flox mice were crossed with GATA1-Cre transgenic mice as described in the text. Recombinant alleles were detected by Southern blotting as described above or by PCR as described in Protocol S1."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T12056","span":{"begin":9,"end":13},"obj":"PR_EXT:000004503"},{"id":"T12057","span":{"begin":14,"end":18},"obj":"SO:0000359"},{"id":"T12058","span":{"begin":19,"end":26},"obj":"CL:0002322"},{"id":"T12059","span":{"begin":22,"end":26},"obj":"CL_GO_EXT:cell"},{"id":"T12060","span":{"begin":27,"end":33},"obj":"SO_EXT:sequence_cloned_entity"},{"id":"T12061","span":{"begin":61,"end":72},"obj":"UBERON:0000358"},{"id":"T12062","span":{"begin":77,"end":88},"obj":"GO_EXT:biological_movement_or_translocation_process"},{"id":"T12063","span":{"begin":176,"end":181},"obj":"GO:0007618"},{"id":"T12064","span":{"begin":250,"end":254},"obj":"PR_EXT:000004503"},{"id":"T12065","span":{"begin":255,"end":259},"obj":"SO:0000359"},{"id":"T12066","span":{"begin":260,"end":266},"obj":"SO_EXT:0001023"},{"id":"T12067","span":{"begin":321,"end":325},"obj":"UBERON:0002415"},{"id":"T12068","span":{"begin":326,"end":329},"obj":"CHEBI_SO_EXT:DNA"},{"id":"T12069","span":{"begin":340,"end":346},"obj":"SO_EXT:0000188"},{"id":"T12070","span":{"begin":350,"end":355},"obj":"CHEBI_SO_EXT:molecular_probe"},{"id":"T12071","span":{"begin":394,"end":407},"obj":"GO_SO_EXT:sequence_rearrangement_process"},{"id":"T12072","span":{"begin":409,"end":413},"obj":"PR_EXT:000004503"},{"id":"T12073","span":{"begin":414,"end":418},"obj":"SO:0000359"},{"id":"T12074","span":{"begin":419,"end":423},"obj":"NCBITaxon:10088"},{"id":"T12075","span":{"begin":442,"end":447},"obj":"PR_EXT:000007857"},{"id":"T12076","span":{"begin":452,"end":462},"obj":"SO_EXT:transgenic_entity"},{"id":"T12077","span":{"begin":463,"end":467},"obj":"NCBITaxon:10088"},{"id":"T12078","span":{"begin":494,"end":505},"obj":"GO_SO_EXT:sequence_rearrangement_process"},{"id":"T12079","span":{"begin":506,"end":513},"obj":"SO_EXT:0001023"}],"text":"Targeted Atrx flox ES cell clones were injected into C57BL/6 blastocysts and transferred into 2.5 dpc pseudopregnant recipients by standard techniques. Resulting chimeras were mated with C57BL/6 to establish germline transmission. Offspring with the Atrx flox allele were identified by Southern blotting of SacI-digested tail DNA using the intron 17 probe as shown in Figure 2A and 2C. For Cre-recombination, Atrx flox mice were crossed with GATA1-Cre transgenic mice as described in the text. Recombinant alleles were detected by Southern blotting as described above or by PCR as described in Protocol S1."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T12036","span":{"begin":9,"end":13},"obj":"PR:000004503"},{"id":"T12037","span":{"begin":14,"end":18},"obj":"SO:0000359"},{"id":"T12038","span":{"begin":19,"end":26},"obj":"CL:0002322"},{"id":"T12039","span":{"begin":61,"end":72},"obj":"UBERON:0000358"},{"id":"T12040","span":{"begin":176,"end":181},"obj":"GO:0007618"},{"id":"T12041","span":{"begin":250,"end":254},"obj":"PR:000004503"},{"id":"T12042","span":{"begin":255,"end":259},"obj":"SO:0000359"},{"id":"T12043","span":{"begin":260,"end":266},"obj":"SO:0001023"},{"id":"T12044","span":{"begin":321,"end":325},"obj":"UBERON:0002415"},{"id":"T12045","span":{"begin":340,"end":346},"obj":"SO:0000188"},{"id":"T12046","span":{"begin":409,"end":413},"obj":"PR:000004503"},{"id":"T12047","span":{"begin":414,"end":418},"obj":"SO:0000359"},{"id":"T12048","span":{"begin":419,"end":423},"obj":"NCBITaxon:10088"},{"id":"T12049","span":{"begin":442,"end":447},"obj":"PR:000007857"},{"id":"T12050","span":{"begin":463,"end":467},"obj":"NCBITaxon:10088"},{"id":"T12051","span":{"begin":506,"end":513},"obj":"SO:0001023"}],"text":"Targeted Atrx flox ES cell clones were injected into C57BL/6 blastocysts and transferred into 2.5 dpc pseudopregnant recipients by standard techniques. Resulting chimeras were mated with C57BL/6 to establish germline transmission. Offspring with the Atrx flox allele were identified by Southern blotting of SacI-digested tail DNA using the intron 17 probe as shown in Figure 2A and 2C. For Cre-recombination, Atrx flox mice were crossed with GATA1-Cre transgenic mice as described in the text. Recombinant alleles were detected by Southern blotting as described above or by PCR as described in Protocol S1."}