Id |
Subject |
Object |
Predicate |
Lexical cue |
T12080 |
0-10 |
NN |
denotes |
Generation |
T12081 |
11-13 |
IN |
denotes |
of |
T12082 |
14-22 |
NNS |
denotes |
chimeras |
T12083 |
22-24 |
, |
denotes |
, |
T12084 |
24-30 |
VBN |
denotes |
floxed |
T12085 |
31-35 |
NNS |
denotes |
mice |
T12086 |
35-37 |
, |
denotes |
, |
T12087 |
37-40 |
CC |
denotes |
and |
T12088 |
41-47 |
NN |
denotes |
mutant |
T12089 |
48-52 |
NNS |
denotes |
mice |
T12090 |
52-53 |
. |
denotes |
. |
T12091 |
53-205 |
sentence |
denotes |
Targeted Atrx flox ES cell clones were injected into C57BL/6 blastocysts and transferred into 2.5 dpc pseudopregnant recipients by standard techniques. |
T12092 |
54-62 |
VBN |
denotes |
Targeted |
T12093 |
81-87 |
NNS |
denotes |
clones |
T12094 |
63-72 |
NN |
denotes |
Atrx flox |
T12095 |
76-80 |
NN |
denotes |
cell |
T12096 |
73-75 |
NN |
denotes |
ES |
T12097 |
93-101 |
VBN |
denotes |
injected |
T12098 |
88-92 |
VBD |
denotes |
were |
T12099 |
102-106 |
IN |
denotes |
into |
T12100 |
107-112 |
NN |
denotes |
C57BL |
T12101 |
115-126 |
NNS |
denotes |
blastocysts |
T12102 |
112-113 |
HYPH |
denotes |
/ |
T12103 |
113-114 |
CD |
denotes |
6 |
T12104 |
127-130 |
CC |
denotes |
and |
T12105 |
131-142 |
VBN |
denotes |
transferred |
T12106 |
143-147 |
IN |
denotes |
into |
T12107 |
148-151 |
CD |
denotes |
2.5 |
T12108 |
152-155 |
NN |
denotes |
dpc |
T12109 |
171-181 |
NNS |
denotes |
recipients |
T12110 |
156-170 |
JJ |
denotes |
pseudopregnant |
T12111 |
182-184 |
IN |
denotes |
by |
T12112 |
185-193 |
JJ |
denotes |
standard |
T12113 |
194-204 |
NNS |
denotes |
techniques |
T12114 |
204-205 |
. |
denotes |
. |
T12115 |
205-284 |
sentence |
denotes |
Resulting chimeras were mated with C57BL/6 to establish germline transmission. |
T12116 |
206-215 |
VBG |
denotes |
Resulting |
T12117 |
216-224 |
NNS |
denotes |
chimeras |
T12118 |
230-235 |
VBN |
denotes |
mated |
T12119 |
225-229 |
VBD |
denotes |
were |
T12120 |
236-240 |
IN |
denotes |
with |
T12121 |
241-246 |
NN |
denotes |
C57BL |
T12122 |
246-247 |
HYPH |
denotes |
/ |
T12123 |
247-248 |
CD |
denotes |
6 |
T12124 |
249-251 |
TO |
denotes |
to |
T12125 |
252-261 |
VB |
denotes |
establish |
T12126 |
262-270 |
NN |
denotes |
germline |
T12127 |
271-283 |
NN |
denotes |
transmission |
T12128 |
283-284 |
. |
denotes |
. |
T12129 |
284-439 |
sentence |
denotes |
Offspring with the Atrx flox allele were identified by Southern blotting of SacI-digested tail DNA using the intron 17 probe as shown in Figure 2A and 2C. |
T12130 |
285-294 |
NN |
denotes |
Offspring |
T12131 |
326-336 |
VBN |
denotes |
identified |
T12132 |
295-299 |
IN |
denotes |
with |
T12133 |
300-303 |
DT |
denotes |
the |
T12134 |
314-320 |
NN |
denotes |
allele |
T12135 |
304-313 |
NN |
denotes |
Atrx flox |
T12136 |
321-325 |
VBD |
denotes |
were |
T12137 |
337-339 |
IN |
denotes |
by |
T12138 |
340-348 |
NNP |
denotes |
Southern |
T12139 |
349-357 |
NN |
denotes |
blotting |
T12140 |
358-360 |
IN |
denotes |
of |
T12141 |
361-365 |
NN |
denotes |
SacI |
T12142 |
366-374 |
VBN |
denotes |
digested |
T12143 |
365-366 |
HYPH |
denotes |
- |
T12144 |
380-383 |
NN |
denotes |
DNA |
T12145 |
375-379 |
NN |
denotes |
tail |
T12146 |
384-389 |
VBG |
denotes |
using |
T12147 |
390-393 |
DT |
denotes |
the |
T12148 |
404-409 |
NN |
denotes |
probe |
T12149 |
394-400 |
NN |
denotes |
intron |
T12150 |
401-403 |
CD |
denotes |
17 |
T12151 |
410-412 |
IN |
denotes |
as |
T12152 |
413-418 |
VBN |
denotes |
shown |
T12153 |
419-421 |
IN |
denotes |
in |
T12154 |
422-428 |
NN |
denotes |
Figure |
T12155 |
429-431 |
NN |
denotes |
2A |
T12156 |
432-435 |
CC |
denotes |
and |
T12157 |
436-438 |
NN |
denotes |
2C |
T12158 |
438-439 |
. |
denotes |
. |
T12159 |
439-547 |
sentence |
denotes |
For Cre-recombination, Atrx flox mice were crossed with GATA1-Cre transgenic mice as described in the text. |
T12160 |
440-443 |
IN |
denotes |
For |
T12161 |
483-490 |
VBN |
denotes |
crossed |
T12162 |
444-447 |
NN |
denotes |
Cre |
T12163 |
448-461 |
NN |
denotes |
recombination |
T12164 |
447-448 |
HYPH |
denotes |
- |
T12165 |
461-463 |
, |
denotes |
, |
T12166 |
463-472 |
NN |
denotes |
Atrx flox |
T12167 |
473-477 |
NNS |
denotes |
mice |
T12168 |
478-482 |
VBD |
denotes |
were |
T12169 |
491-495 |
IN |
denotes |
with |
T12170 |
496-501 |
NN |
denotes |
GATA1 |
T12171 |
502-505 |
NN |
denotes |
Cre |
T12172 |
501-502 |
HYPH |
denotes |
- |
T12173 |
506-516 |
JJ |
denotes |
transgenic |
T12174 |
517-521 |
NNS |
denotes |
mice |
T12175 |
522-524 |
IN |
denotes |
as |
T12176 |
525-534 |
VBN |
denotes |
described |
T12177 |
535-537 |
IN |
denotes |
in |
T12178 |
538-541 |
DT |
denotes |
the |
T12179 |
542-546 |
NN |
denotes |
text |
T12180 |
546-547 |
. |
denotes |
. |
T12181 |
547-660 |
sentence |
denotes |
Recombinant alleles were detected by Southern blotting as described above or by PCR as described in Protocol S1. |
T12182 |
548-559 |
JJ |
denotes |
Recombinant |
T12183 |
560-567 |
NNS |
denotes |
alleles |
T12184 |
573-581 |
VBN |
denotes |
detected |
T12185 |
568-572 |
VBD |
denotes |
were |
T12186 |
582-584 |
IN |
denotes |
by |
T12187 |
585-593 |
NNP |
denotes |
Southern |
T12188 |
594-602 |
NN |
denotes |
blotting |
T12189 |
603-605 |
IN |
denotes |
as |
T12190 |
606-615 |
VBN |
denotes |
described |
T12191 |
616-621 |
RB |
denotes |
above |
T12192 |
622-624 |
CC |
denotes |
or |
T12193 |
625-627 |
IN |
denotes |
by |
T12194 |
628-631 |
NN |
denotes |
PCR |
T12195 |
632-634 |
IN |
denotes |
as |
T12196 |
635-644 |
VBN |
denotes |
described |
T12197 |
645-647 |
IN |
denotes |
in |
T12198 |
648-656 |
NN |
denotes |
Protocol |
T12199 |
657-659 |
NN |
denotes |
S1 |
T12200 |
659-660 |
. |
denotes |
. |
R7552 |
T12081 |
T12080 |
prep |
of,Generation |
R7553 |
T12082 |
T12081 |
pobj |
chimeras,of |
R7554 |
T12083 |
T12082 |
punct |
", ",chimeras |
R7555 |
T12084 |
T12085 |
amod |
floxed,mice |
R7556 |
T12085 |
T12082 |
conj |
mice,chimeras |
R7557 |
T12086 |
T12085 |
punct |
", ",mice |
R7558 |
T12087 |
T12085 |
cc |
and,mice |
R7559 |
T12088 |
T12089 |
compound |
mutant,mice |
R7560 |
T12089 |
T12085 |
conj |
mice,mice |
R7561 |
T12090 |
T12080 |
punct |
.,Generation |
R7562 |
T12092 |
T12093 |
amod |
Targeted,clones |
R7563 |
T12093 |
T12097 |
nsubjpass |
clones,injected |
R7564 |
T12094 |
T12095 |
compound |
Atrx flox,cell |
R7565 |
T12095 |
T12093 |
compound |
cell,clones |
R7566 |
T12096 |
T12095 |
compound |
ES,cell |
R7567 |
T12098 |
T12097 |
auxpass |
were,injected |
R7568 |
T12099 |
T12097 |
prep |
into,injected |
R7569 |
T12100 |
T12101 |
nmod |
C57BL,blastocysts |
R7570 |
T12101 |
T12099 |
pobj |
blastocysts,into |
R7571 |
T12102 |
T12100 |
punct |
/,C57BL |
R7572 |
T12103 |
T12100 |
nummod |
6,C57BL |
R7573 |
T12104 |
T12097 |
cc |
and,injected |
R7574 |
T12105 |
T12097 |
conj |
transferred,injected |
R7575 |
T12106 |
T12105 |
prep |
into,transferred |
R7576 |
T12107 |
T12108 |
nummod |
2.5,dpc |
R7577 |
T12108 |
T12109 |
nmod |
dpc,recipients |
R7578 |
T12109 |
T12106 |
pobj |
recipients,into |
R7579 |
T12110 |
T12109 |
amod |
pseudopregnant,recipients |
R7580 |
T12111 |
T12097 |
prep |
by,injected |
R7581 |
T12112 |
T12113 |
amod |
standard,techniques |
R7582 |
T12113 |
T12111 |
pobj |
techniques,by |
R7583 |
T12114 |
T12097 |
punct |
.,injected |
R7584 |
T12116 |
T12117 |
amod |
Resulting,chimeras |
R7585 |
T12117 |
T12118 |
nsubjpass |
chimeras,mated |
R7586 |
T12119 |
T12118 |
auxpass |
were,mated |
R7587 |
T12120 |
T12118 |
prep |
with,mated |
R7588 |
T12121 |
T12120 |
pobj |
C57BL,with |
R7589 |
T12122 |
T12121 |
punct |
/,C57BL |
R7590 |
T12123 |
T12121 |
nummod |
6,C57BL |
R7591 |
T12124 |
T12125 |
aux |
to,establish |
R7592 |
T12125 |
T12118 |
advcl |
establish,mated |
R7593 |
T12126 |
T12127 |
compound |
germline,transmission |
R7594 |
T12127 |
T12125 |
dobj |
transmission,establish |
R7595 |
T12128 |
T12118 |
punct |
.,mated |
R7596 |
T12130 |
T12131 |
nsubjpass |
Offspring,identified |
R7597 |
T12132 |
T12130 |
prep |
with,Offspring |
R7598 |
T12133 |
T12134 |
det |
the,allele |
R7599 |
T12134 |
T12132 |
pobj |
allele,with |
R7600 |
T12135 |
T12134 |
compound |
Atrx flox,allele |
R7601 |
T12136 |
T12131 |
auxpass |
were,identified |
R7602 |
T12137 |
T12131 |
prep |
by,identified |
R7603 |
T12138 |
T12139 |
compound |
Southern,blotting |
R7604 |
T12139 |
T12137 |
pobj |
blotting,by |
R7605 |
T12140 |
T12139 |
prep |
of,blotting |
R7606 |
T12141 |
T12142 |
npadvmod |
SacI,digested |
R7607 |
T12142 |
T12144 |
amod |
digested,DNA |
R7608 |
T12143 |
T12142 |
punct |
-,digested |
R7609 |
T12144 |
T12140 |
pobj |
DNA,of |
R7610 |
T12145 |
T12144 |
compound |
tail,DNA |
R7611 |
T12146 |
T12131 |
advcl |
using,identified |
R7612 |
T12147 |
T12148 |
det |
the,probe |
R7613 |
T12148 |
T12146 |
dobj |
probe,using |
R7614 |
T12149 |
T12148 |
nmod |
intron,probe |
R7615 |
T12150 |
T12149 |
nummod |
17,intron |
R7616 |
T12151 |
T12152 |
mark |
as,shown |
R7617 |
T12152 |
T12146 |
advcl |
shown,using |
R7618 |
T12153 |
T12152 |
prep |
in,shown |
R7619 |
T12154 |
T12155 |
compound |
Figure,2A |
R7620 |
T12155 |
T12153 |
pobj |
2A,in |
R7621 |
T12156 |
T12155 |
cc |
and,2A |
R7622 |
T12157 |
T12155 |
conj |
2C,2A |
R7623 |
T12158 |
T12131 |
punct |
.,identified |
R7624 |
T12160 |
T12161 |
prep |
For,crossed |
R7625 |
T12162 |
T12163 |
compound |
Cre,recombination |
R7626 |
T12163 |
T12160 |
pobj |
recombination,For |
R7627 |
T12164 |
T12163 |
punct |
-,recombination |
R7628 |
T12165 |
T12161 |
punct |
", ",crossed |
R7629 |
T12166 |
T12167 |
compound |
Atrx flox,mice |
R7630 |
T12167 |
T12161 |
nsubjpass |
mice,crossed |
R7631 |
T12168 |
T12161 |
auxpass |
were,crossed |
R7632 |
T12169 |
T12161 |
prep |
with,crossed |
R7633 |
T12170 |
T12171 |
compound |
GATA1,Cre |
R7634 |
T12171 |
T12173 |
npadvmod |
Cre,transgenic |
R7635 |
T12172 |
T12171 |
punct |
-,Cre |
R7636 |
T12173 |
T12174 |
amod |
transgenic,mice |
R7637 |
T12174 |
T12169 |
pobj |
mice,with |
R7638 |
T12175 |
T12176 |
mark |
as,described |
R7639 |
T12176 |
T12161 |
advcl |
described,crossed |
R7640 |
T12177 |
T12176 |
prep |
in,described |
R7641 |
T12178 |
T12179 |
det |
the,text |
R7642 |
T12179 |
T12177 |
pobj |
text,in |
R7643 |
T12180 |
T12161 |
punct |
.,crossed |
R7644 |
T12182 |
T12183 |
amod |
Recombinant,alleles |
R7645 |
T12183 |
T12184 |
nsubjpass |
alleles,detected |
R7646 |
T12185 |
T12184 |
auxpass |
were,detected |
R7647 |
T12186 |
T12184 |
prep |
by,detected |
R7648 |
T12187 |
T12188 |
compound |
Southern,blotting |
R7649 |
T12188 |
T12186 |
pobj |
blotting,by |
R7650 |
T12189 |
T12190 |
mark |
as,described |
R7651 |
T12190 |
T12188 |
advcl |
described,blotting |
R7652 |
T12191 |
T12190 |
advmod |
above,described |
R7653 |
T12192 |
T12186 |
cc |
or,by |
R7654 |
T12193 |
T12186 |
conj |
by,by |
R7655 |
T12194 |
T12193 |
pobj |
PCR,by |
R7656 |
T12195 |
T12196 |
mark |
as,described |
R7657 |
T12196 |
T12184 |
advcl |
described,detected |
R7658 |
T12197 |
T12196 |
prep |
in,described |
R7659 |
T12198 |
T12199 |
compound |
Protocol,S1 |
R7660 |
T12199 |
T12197 |
pobj |
S1,in |
R7661 |
T12200 |
T12184 |
punct |
.,detected |