Id |
Subject |
Object |
Predicate |
Lexical cue |
T11583 |
0-2 |
NN |
denotes |
ES |
T11584 |
3-7 |
NN |
denotes |
cell |
T11585 |
8-14 |
NNS |
denotes |
clones |
T11586 |
77-88 |
VBN |
denotes |
transfected |
T11587 |
15-22 |
VBG |
denotes |
bearing |
T11588 |
23-26 |
DT |
denotes |
the |
T11589 |
37-43 |
NN |
denotes |
allele |
T11590 |
27-36 |
NN |
denotes |
Atrx flox |
T11591 |
44-45 |
-LRB- |
denotes |
( |
T11592 |
53-58 |
NNS |
denotes |
cells |
T11593 |
45-46 |
CD |
denotes |
1 |
T11594 |
49-52 |
CD |
denotes |
107 |
T11595 |
47-48 |
SYM |
denotes |
× |
T11596 |
58-59 |
-RRB- |
denotes |
) |
T11597 |
60-64 |
VBD |
denotes |
were |
T11598 |
65-76 |
RB |
denotes |
transiently |
T11599 |
89-93 |
IN |
denotes |
with |
T11600 |
94-96 |
CD |
denotes |
50 |
T11601 |
97-99 |
NNS |
denotes |
μg |
T11602 |
100-102 |
IN |
denotes |
of |
T11603 |
103-108 |
JJ |
denotes |
uncut |
T11604 |
124-131 |
NN |
denotes |
plasmid |
T11605 |
109-112 |
NN |
denotes |
Cre |
T11606 |
113-123 |
NN |
denotes |
expression |
T11607 |
132-133 |
-LRB- |
denotes |
( |
T11608 |
133-139 |
NN |
denotes |
pCAGGS |
T11609 |
144-152 |
NN |
denotes |
IRESpuro |
T11610 |
139-140 |
HYPH |
denotes |
- |
T11611 |
140-143 |
NN |
denotes |
Cre |
T11612 |
143-144 |
HYPH |
denotes |
- |
T11613 |
152-153 |
-RRB- |
denotes |
) |
T11614 |
154-155 |
-LRB- |
denotes |
[ |
T11615 |
155-157 |
CD |
denotes |
40 |
T11616 |
157-158 |
-RRB- |
denotes |
] |
T11617 |
158-159 |
. |
denotes |
. |
T11618 |
159-311 |
sentence |
denotes |
Following transfection, cells were plated at a range of clonal densities in complete medium without G418, and isolated subclones were picked after 7 d. |
T11619 |
160-169 |
VBG |
denotes |
Following |
T11620 |
195-201 |
VBN |
denotes |
plated |
T11621 |
170-182 |
NN |
denotes |
transfection |
T11622 |
182-184 |
, |
denotes |
, |
T11623 |
184-189 |
NNS |
denotes |
cells |
T11624 |
190-194 |
VBD |
denotes |
were |
T11625 |
202-204 |
IN |
denotes |
at |
T11626 |
205-206 |
DT |
denotes |
a |
T11627 |
207-212 |
NN |
denotes |
range |
T11628 |
213-215 |
IN |
denotes |
of |
T11629 |
216-222 |
JJ |
denotes |
clonal |
T11630 |
223-232 |
NNS |
denotes |
densities |
T11631 |
233-235 |
IN |
denotes |
in |
T11632 |
236-244 |
JJ |
denotes |
complete |
T11633 |
245-251 |
NN |
denotes |
medium |
T11634 |
252-259 |
IN |
denotes |
without |
T11635 |
260-264 |
NN |
denotes |
G418 |
T11636 |
264-266 |
, |
denotes |
, |
T11637 |
266-269 |
CC |
denotes |
and |
T11638 |
270-278 |
VBN |
denotes |
isolated |
T11639 |
279-288 |
NNS |
denotes |
subclones |
T11640 |
294-300 |
VBN |
denotes |
picked |
T11641 |
289-293 |
VBD |
denotes |
were |
T11642 |
301-306 |
IN |
denotes |
after |
T11643 |
307-308 |
CD |
denotes |
7 |
T11644 |
309-310 |
NNS |
denotes |
d |
T11645 |
310-311 |
. |
denotes |
. |
T11646 |
311-556 |
sentence |
denotes |
Subclones were expanded and analysed for the presence of a recombinant locus initially by PCR, to detect deletion of the MC1neopA cassette, and then by Southern blot and hybridisation with the intron 17 probe described above (Figure 2A and 2C). |
T11647 |
312-321 |
NNS |
denotes |
Subclones |
T11648 |
327-335 |
VBN |
denotes |
expanded |
T11649 |
322-326 |
VBD |
denotes |
were |
T11650 |
336-339 |
CC |
denotes |
and |
T11651 |
340-348 |
VBN |
denotes |
analysed |
T11652 |
349-352 |
IN |
denotes |
for |
T11653 |
353-356 |
DT |
denotes |
the |
T11654 |
357-365 |
NN |
denotes |
presence |
T11655 |
366-368 |
IN |
denotes |
of |
T11656 |
369-370 |
DT |
denotes |
a |
T11657 |
383-388 |
NN |
denotes |
locus |
T11658 |
371-382 |
JJ |
denotes |
recombinant |
T11659 |
389-398 |
RB |
denotes |
initially |
T11660 |
399-401 |
IN |
denotes |
by |
T11661 |
402-405 |
NN |
denotes |
PCR |
T11662 |
405-407 |
, |
denotes |
, |
T11663 |
407-409 |
TO |
denotes |
to |
T11664 |
410-416 |
VB |
denotes |
detect |
T11665 |
417-425 |
NN |
denotes |
deletion |
T11666 |
426-428 |
IN |
denotes |
of |
T11667 |
429-432 |
DT |
denotes |
the |
T11668 |
442-450 |
NN |
denotes |
cassette |
T11669 |
433-441 |
NN |
denotes |
MC1neopA |
T11670 |
450-452 |
, |
denotes |
, |
T11671 |
452-455 |
CC |
denotes |
and |
T11672 |
456-460 |
RB |
denotes |
then |
T11673 |
461-463 |
IN |
denotes |
by |
T11674 |
464-472 |
NNP |
denotes |
Southern |
T11675 |
473-477 |
NN |
denotes |
blot |
T11676 |
478-481 |
CC |
denotes |
and |
T11677 |
482-495 |
NN |
denotes |
hybridisation |
T11678 |
496-500 |
IN |
denotes |
with |
T11679 |
501-504 |
DT |
denotes |
the |
T11680 |
515-520 |
NN |
denotes |
probe |
T11681 |
505-511 |
NN |
denotes |
intron |
T11682 |
512-514 |
CD |
denotes |
17 |
T11683 |
521-530 |
VBN |
denotes |
described |
T11684 |
531-536 |
RB |
denotes |
above |
T11685 |
537-538 |
-LRB- |
denotes |
( |
T11686 |
545-547 |
NN |
denotes |
2A |
T11687 |
538-544 |
NN |
denotes |
Figure |
T11688 |
548-551 |
CC |
denotes |
and |
T11689 |
552-554 |
NN |
denotes |
2C |
T11690 |
554-555 |
-RRB- |
denotes |
) |
T11691 |
555-556 |
. |
denotes |
. |
T11692 |
556-726 |
sentence |
denotes |
Northern blots were carried out according to standard techniques using 20 μg of total RNA isolated using TRI Reagent (Sigma-Aldrich, St. Louis, Missouri, United States). |
T11693 |
557-565 |
NNP |
denotes |
Northern |
T11694 |
566-571 |
NNS |
denotes |
blots |
T11695 |
577-584 |
VBN |
denotes |
carried |
T11696 |
572-576 |
VBD |
denotes |
were |
T11697 |
585-588 |
RP |
denotes |
out |
T11698 |
589-598 |
VBG |
denotes |
according |
T11699 |
599-601 |
IN |
denotes |
to |
T11700 |
602-610 |
JJ |
denotes |
standard |
T11701 |
611-621 |
NNS |
denotes |
techniques |
T11702 |
622-627 |
VBG |
denotes |
using |
T11703 |
628-630 |
CD |
denotes |
20 |
T11704 |
631-633 |
NNS |
denotes |
μg |
T11705 |
634-636 |
IN |
denotes |
of |
T11706 |
637-642 |
JJ |
denotes |
total |
T11707 |
643-646 |
NN |
denotes |
RNA |
T11708 |
647-655 |
VBN |
denotes |
isolated |
T11709 |
656-661 |
VBG |
denotes |
using |
T11710 |
662-665 |
NN |
denotes |
TRI |
T11711 |
666-673 |
NN |
denotes |
Reagent |
T11712 |
674-675 |
-LRB- |
denotes |
( |
T11713 |
681-688 |
NNP |
denotes |
Aldrich |
T11714 |
675-680 |
NNP |
denotes |
Sigma |
T11715 |
680-681 |
HYPH |
denotes |
- |
T11716 |
688-690 |
, |
denotes |
, |
T11717 |
690-693 |
NNP |
denotes |
St. |
T11718 |
694-699 |
NNP |
denotes |
Louis |
T11719 |
699-701 |
, |
denotes |
, |
T11720 |
701-709 |
NNP |
denotes |
Missouri |
T11721 |
709-711 |
, |
denotes |
, |
T11722 |
711-717 |
NNP |
denotes |
United |
T11723 |
718-724 |
NNP |
denotes |
States |
T11724 |
724-725 |
-RRB- |
denotes |
) |
T11725 |
725-726 |
. |
denotes |
. |
T11726 |
726-854 |
sentence |
denotes |
The blot was hybridised with a probe from within exon 10 of the Atrx gene (generated with primers Mxnp4 and Mxnp28 [Table S1]). |
T11727 |
727-730 |
DT |
denotes |
The |
T11728 |
731-735 |
NN |
denotes |
blot |
T11729 |
740-750 |
VBN |
denotes |
hybridised |
T11730 |
736-739 |
VBD |
denotes |
was |
T11731 |
751-755 |
IN |
denotes |
with |
T11732 |
756-757 |
DT |
denotes |
a |
T11733 |
758-763 |
NN |
denotes |
probe |
T11734 |
764-768 |
IN |
denotes |
from |
T11735 |
769-775 |
IN |
denotes |
within |
T11736 |
776-780 |
NN |
denotes |
exon |
T11737 |
781-783 |
CD |
denotes |
10 |
T11738 |
784-786 |
IN |
denotes |
of |
T11739 |
787-790 |
DT |
denotes |
the |
T11740 |
796-800 |
NN |
denotes |
gene |
T11741 |
791-795 |
NN |
denotes |
Atrx |
T11742 |
801-802 |
-LRB- |
denotes |
( |
T11743 |
802-811 |
VBN |
denotes |
generated |
T11744 |
812-816 |
IN |
denotes |
with |
T11745 |
817-824 |
NNS |
denotes |
primers |
T11746 |
825-830 |
NN |
denotes |
Mxnp4 |
T11747 |
831-834 |
CC |
denotes |
and |
T11748 |
835-841 |
NN |
denotes |
Mxnp28 |
T11749 |
842-843 |
-LRB- |
denotes |
[ |
T11750 |
849-851 |
NN |
denotes |
S1 |
T11751 |
843-848 |
NN |
denotes |
Table |
T11752 |
851-852 |
-RRB- |
denotes |
] |
T11753 |
852-853 |
-RRB- |
denotes |
) |
T11754 |
853-854 |
. |
denotes |
. |
T11755 |
854-981 |
sentence |
denotes |
After it was stripped, the membrane was hybridised with a β-actin cDNA probe (Clontech, Palo Alto, California, United States). |
T11756 |
855-860 |
IN |
denotes |
After |
T11757 |
868-876 |
VBN |
denotes |
stripped |
T11758 |
861-863 |
PRP |
denotes |
it |
T11759 |
864-867 |
VBD |
denotes |
was |
T11760 |
895-905 |
VBN |
denotes |
hybridised |
T11761 |
876-878 |
, |
denotes |
, |
T11762 |
878-881 |
DT |
denotes |
the |
T11763 |
882-890 |
NN |
denotes |
membrane |
T11764 |
891-894 |
VBD |
denotes |
was |
T11765 |
906-910 |
IN |
denotes |
with |
T11766 |
911-912 |
DT |
denotes |
a |
T11767 |
926-931 |
NN |
denotes |
probe |
T11768 |
913-914 |
NN |
denotes |
β |
T11769 |
915-920 |
NN |
denotes |
actin |
T11770 |
914-915 |
HYPH |
denotes |
- |
T11771 |
921-925 |
NN |
denotes |
cDNA |
T11772 |
932-933 |
-LRB- |
denotes |
( |
T11773 |
933-941 |
NNP |
denotes |
Clontech |
T11774 |
941-943 |
, |
denotes |
, |
T11775 |
943-947 |
NNP |
denotes |
Palo |
T11776 |
948-952 |
NNP |
denotes |
Alto |
T11777 |
952-954 |
, |
denotes |
, |
T11778 |
954-964 |
NNP |
denotes |
California |
T11779 |
964-966 |
, |
denotes |
, |
T11780 |
966-972 |
NNP |
denotes |
United |
T11781 |
973-979 |
NNP |
denotes |
States |
T11782 |
979-980 |
-RRB- |
denotes |
) |
T11783 |
980-981 |
. |
denotes |
. |
T11784 |
981-1141 |
sentence |
denotes |
Protein extraction and detection of Atrx by Western blotting was performed as described previously [4], using the mouse monoclonal anti-ATRX antibody 23C [15]. |
T11785 |
982-989 |
NN |
denotes |
Protein |
T11786 |
990-1000 |
NN |
denotes |
extraction |
T11787 |
1047-1056 |
VBN |
denotes |
performed |
T11788 |
1001-1004 |
CC |
denotes |
and |
T11789 |
1005-1014 |
NN |
denotes |
detection |
T11790 |
1015-1017 |
IN |
denotes |
of |
T11791 |
1018-1022 |
NN |
denotes |
Atrx |
T11792 |
1023-1025 |
IN |
denotes |
by |
T11793 |
1026-1033 |
NNP |
denotes |
Western |
T11794 |
1034-1042 |
NN |
denotes |
blotting |
T11795 |
1043-1046 |
VBD |
denotes |
was |
T11796 |
1057-1059 |
IN |
denotes |
as |
T11797 |
1060-1069 |
VBN |
denotes |
described |
T11798 |
1070-1080 |
RB |
denotes |
previously |
T11799 |
1081-1082 |
-LRB- |
denotes |
[ |
T11800 |
1082-1083 |
CD |
denotes |
4 |
T11801 |
1083-1084 |
-RRB- |
denotes |
] |
T11802 |
1084-1086 |
, |
denotes |
, |
T11803 |
1086-1091 |
VBG |
denotes |
using |
T11804 |
1092-1095 |
DT |
denotes |
the |
T11805 |
1123-1131 |
NN |
denotes |
antibody |
T11806 |
1096-1101 |
NN |
denotes |
mouse |
T11807 |
1102-1112 |
JJ |
denotes |
monoclonal |
T11808 |
1113-1122 |
JJ |
denotes |
anti-ATRX |
T11809 |
1132-1135 |
NN |
denotes |
23C |
T11810 |
1136-1137 |
-LRB- |
denotes |
[ |
T11811 |
1137-1139 |
CD |
denotes |
15 |
T11812 |
1139-1140 |
-RRB- |
denotes |
] |
T11813 |
1140-1141 |
. |
denotes |
. |
T11814 |
1141-1208 |
sentence |
denotes |
Analyses of cell cycle and apoptosis are described in Protocol S1. |
T11815 |
1142-1150 |
NNS |
denotes |
Analyses |
T11816 |
1183-1192 |
VBN |
denotes |
described |
T11817 |
1151-1153 |
IN |
denotes |
of |
T11818 |
1154-1158 |
NN |
denotes |
cell |
T11819 |
1159-1164 |
NN |
denotes |
cycle |
T11820 |
1165-1168 |
CC |
denotes |
and |
T11821 |
1169-1178 |
NN |
denotes |
apoptosis |
T11822 |
1179-1182 |
VBP |
denotes |
are |
T11823 |
1193-1195 |
IN |
denotes |
in |
T11824 |
1196-1204 |
NN |
denotes |
Protocol |
T11825 |
1205-1207 |
NN |
denotes |
S1 |
T11826 |
1207-1208 |
. |
denotes |
. |
T11827 |
1208-1370 |
sentence |
denotes |
Methylation of rDNA was analysed in DNA from ES cell clones or from embryoid bodies recovered after 7 d of in vitro differentiation as described previously [41]. |
T11828 |
1209-1220 |
NN |
denotes |
Methylation |
T11829 |
1233-1241 |
VBN |
denotes |
analysed |
T11830 |
1221-1223 |
IN |
denotes |
of |
T11831 |
1224-1228 |
NN |
denotes |
rDNA |
T11832 |
1229-1232 |
VBD |
denotes |
was |
T11833 |
1242-1244 |
IN |
denotes |
in |
T11834 |
1245-1248 |
NN |
denotes |
DNA |
T11835 |
1249-1253 |
IN |
denotes |
from |
T11836 |
1254-1256 |
NN |
denotes |
ES |
T11837 |
1257-1261 |
NN |
denotes |
cell |
T11838 |
1262-1268 |
NNS |
denotes |
clones |
T11839 |
1269-1271 |
CC |
denotes |
or |
T11840 |
1272-1276 |
IN |
denotes |
from |
T11841 |
1277-1285 |
JJ |
denotes |
embryoid |
T11842 |
1286-1292 |
NNS |
denotes |
bodies |
T11843 |
1293-1302 |
VBN |
denotes |
recovered |
T11844 |
1303-1308 |
IN |
denotes |
after |
T11845 |
1309-1310 |
CD |
denotes |
7 |
T11846 |
1311-1312 |
NNS |
denotes |
d |
T11847 |
1313-1315 |
IN |
denotes |
of |
T11848 |
1316-1318 |
FW |
denotes |
in |
T11849 |
1319-1324 |
FW |
denotes |
vitro |
T11850 |
1325-1340 |
NN |
denotes |
differentiation |
T11851 |
1341-1343 |
IN |
denotes |
as |
T11852 |
1344-1353 |
VBN |
denotes |
described |
T11853 |
1354-1364 |
RB |
denotes |
previously |
T11854 |
1365-1366 |
-LRB- |
denotes |
[ |
T11855 |
1366-1368 |
CD |
denotes |
41 |
T11856 |
1368-1369 |
-RRB- |
denotes |
] |
T11857 |
1369-1370 |
. |
denotes |
. |
T11858 |
1370-1490 |
sentence |
denotes |
Genomic DNA was digested with methylation-sensitive restriction enzymes as described and analysed by Southern blotting. |
T11859 |
1371-1378 |
JJ |
denotes |
Genomic |
T11860 |
1379-1382 |
NN |
denotes |
DNA |
T11861 |
1387-1395 |
VBN |
denotes |
digested |
T11862 |
1383-1386 |
VBD |
denotes |
was |
T11863 |
1396-1400 |
IN |
denotes |
with |
T11864 |
1401-1412 |
NN |
denotes |
methylation |
T11865 |
1413-1422 |
JJ |
denotes |
sensitive |
T11866 |
1412-1413 |
HYPH |
denotes |
- |
T11867 |
1435-1442 |
NNS |
denotes |
enzymes |
T11868 |
1423-1434 |
NN |
denotes |
restriction |
T11869 |
1443-1445 |
IN |
denotes |
as |
T11870 |
1446-1455 |
VBN |
denotes |
described |
T11871 |
1456-1459 |
CC |
denotes |
and |
T11872 |
1460-1468 |
VBN |
denotes |
analysed |
T11873 |
1469-1471 |
IN |
denotes |
by |
T11874 |
1472-1480 |
NNP |
denotes |
Southern |
T11875 |
1481-1489 |
NN |
denotes |
blotting |
T11876 |
1489-1490 |
. |
denotes |
. |
T11877 |
1490-1634 |
sentence |
denotes |
The RIB3 and RIB4 probes (which were amplified from human DNA, but cross-react with the mouse rDNA repeat) have been described previously [11]. |
T11878 |
1491-1494 |
DT |
denotes |
The |
T11879 |
1509-1515 |
NNS |
denotes |
probes |
T11880 |
1495-1499 |
NN |
denotes |
RIB3 |
T11881 |
1500-1503 |
CC |
denotes |
and |
T11882 |
1504-1508 |
NN |
denotes |
RIB4 |
T11883 |
1608-1617 |
VBN |
denotes |
described |
T11884 |
1516-1517 |
-LRB- |
denotes |
( |
T11885 |
1517-1522 |
WDT |
denotes |
which |
T11886 |
1528-1537 |
VBN |
denotes |
amplified |
T11887 |
1523-1527 |
VBD |
denotes |
were |
T11888 |
1538-1542 |
IN |
denotes |
from |
T11889 |
1543-1548 |
JJ |
denotes |
human |
T11890 |
1549-1552 |
NN |
denotes |
DNA |
T11891 |
1552-1554 |
, |
denotes |
, |
T11892 |
1554-1557 |
CC |
denotes |
but |
T11893 |
1558-1569 |
VBP |
denotes |
cross-react |
T11894 |
1570-1574 |
IN |
denotes |
with |
T11895 |
1575-1578 |
DT |
denotes |
the |
T11896 |
1590-1596 |
NN |
denotes |
repeat |
T11897 |
1579-1584 |
NN |
denotes |
mouse |
T11898 |
1585-1589 |
NN |
denotes |
rDNA |
T11899 |
1596-1597 |
-RRB- |
denotes |
) |
T11900 |
1598-1602 |
VBP |
denotes |
have |
T11901 |
1603-1607 |
VBN |
denotes |
been |
T11902 |
1618-1628 |
RB |
denotes |
previously |
T11903 |
1629-1630 |
-LRB- |
denotes |
[ |
T11904 |
1630-1632 |
CD |
denotes |
11 |
T11905 |
1632-1633 |
-RRB- |
denotes |
] |
T11906 |
1633-1634 |
. |
denotes |
. |
T11907 |
1634-1731 |
sentence |
denotes |
Oligonucleotide probes to detect Line 1 and Sine B1 repeats have been described previously [42]. |
T11908 |
1635-1650 |
NN |
denotes |
Oligonucleotide |
T11909 |
1651-1657 |
NNS |
denotes |
probes |
T11910 |
1705-1714 |
VBN |
denotes |
described |
T11911 |
1658-1660 |
TO |
denotes |
to |
T11912 |
1661-1667 |
VB |
denotes |
detect |
T11913 |
1668-1672 |
NN |
denotes |
Line |
T11914 |
1687-1694 |
NNS |
denotes |
repeats |
T11915 |
1673-1674 |
CD |
denotes |
1 |
T11916 |
1675-1678 |
CC |
denotes |
and |
T11917 |
1679-1683 |
NN |
denotes |
Sine |
T11918 |
1684-1686 |
NN |
denotes |
B1 |
T11919 |
1695-1699 |
VBP |
denotes |
have |
T11920 |
1700-1704 |
VBN |
denotes |
been |
T11921 |
1715-1725 |
RB |
denotes |
previously |
T11922 |
1726-1727 |
-LRB- |
denotes |
[ |
T11923 |
1727-1729 |
CD |
denotes |
42 |
T11924 |
1729-1730 |
-RRB- |
denotes |
] |
T11925 |
1730-1731 |
. |
denotes |
. |
T11926 |
1731-1796 |
sentence |
denotes |
The minor satellite probe was a 27-mer oligonucleotide (mCENT2). |
T11927 |
1732-1735 |
DT |
denotes |
The |
T11928 |
1752-1757 |
NN |
denotes |
probe |
T11929 |
1736-1741 |
JJ |
denotes |
minor |
T11930 |
1742-1751 |
NN |
denotes |
satellite |
T11931 |
1758-1761 |
VBD |
denotes |
was |
T11932 |
1762-1763 |
DT |
denotes |
a |
T11933 |
1771-1786 |
NN |
denotes |
oligonucleotide |
T11934 |
1764-1766 |
CD |
denotes |
27 |
T11935 |
1767-1770 |
NN |
denotes |
mer |
T11936 |
1766-1767 |
HYPH |
denotes |
- |
T11937 |
1787-1788 |
-LRB- |
denotes |
( |
T11938 |
1788-1794 |
NN |
denotes |
mCENT2 |
T11939 |
1794-1795 |
-RRB- |
denotes |
) |
T11940 |
1795-1796 |
. |
denotes |
. |
T11941 |
1796-1859 |
sentence |
denotes |
The major satellite probe was a 27-mer oligonucleotide (DG27). |
T11942 |
1797-1800 |
DT |
denotes |
The |
T11943 |
1817-1822 |
NN |
denotes |
probe |
T11944 |
1801-1806 |
JJ |
denotes |
major |
T11945 |
1807-1816 |
NN |
denotes |
satellite |
T11946 |
1823-1826 |
VBD |
denotes |
was |
T11947 |
1827-1828 |
DT |
denotes |
a |
T11948 |
1836-1851 |
NN |
denotes |
oligonucleotide |
T11949 |
1829-1831 |
CD |
denotes |
27 |
T11950 |
1832-1835 |
NN |
denotes |
mer |
T11951 |
1831-1832 |
HYPH |
denotes |
- |
T11952 |
1852-1853 |
-LRB- |
denotes |
( |
T11953 |
1853-1857 |
NN |
denotes |
DG27 |
T11954 |
1857-1858 |
-RRB- |
denotes |
) |
T11955 |
1858-1859 |
. |
denotes |
. |
T11956 |
1859-2036 |
sentence |
denotes |
The IAP probe was an ~400 bp PCR product (primers 14A and 13K) amplified from an IAP inserted into the mouse agouti gene [43] and was a gift from Peter Warnecke and Tim Bestor. |
T11957 |
1860-1863 |
DT |
denotes |
The |
T11958 |
1868-1873 |
NN |
denotes |
probe |
T11959 |
1864-1867 |
NN |
denotes |
IAP |
T11960 |
1874-1877 |
VBD |
denotes |
was |
T11961 |
1878-1880 |
DT |
denotes |
an |
T11962 |
1893-1900 |
NN |
denotes |
product |
T11963 |
1881-1882 |
SYM |
denotes |
~ |
T11964 |
1882-1885 |
CD |
denotes |
400 |
T11965 |
1886-1888 |
NN |
denotes |
bp |
T11966 |
1889-1892 |
NN |
denotes |
PCR |
T11967 |
1901-1902 |
-LRB- |
denotes |
( |
T11968 |
1902-1909 |
NNS |
denotes |
primers |
T11969 |
1910-1913 |
NN |
denotes |
14A |
T11970 |
1914-1917 |
CC |
denotes |
and |
T11971 |
1918-1921 |
NN |
denotes |
13K |
T11972 |
1921-1922 |
-RRB- |
denotes |
) |
T11973 |
1923-1932 |
VBN |
denotes |
amplified |
T11974 |
1933-1937 |
IN |
denotes |
from |
T11975 |
1938-1940 |
DT |
denotes |
an |
T11976 |
1941-1944 |
NN |
denotes |
IAP |
T11977 |
1945-1953 |
VBN |
denotes |
inserted |
T11978 |
1954-1958 |
IN |
denotes |
into |
T11979 |
1959-1962 |
DT |
denotes |
the |
T11980 |
1976-1980 |
NN |
denotes |
gene |
T11981 |
1963-1968 |
NN |
denotes |
mouse |
T11982 |
1969-1975 |
NN |
denotes |
agouti |
T11983 |
1981-1982 |
-LRB- |
denotes |
[ |
T11984 |
1982-1984 |
CD |
denotes |
43 |
T11985 |
1984-1985 |
-RRB- |
denotes |
] |
T11986 |
1986-1989 |
CC |
denotes |
and |
T11987 |
1990-1993 |
VBD |
denotes |
was |
T11988 |
1994-1995 |
DT |
denotes |
a |
T11989 |
1996-2000 |
NN |
denotes |
gift |
T11990 |
2001-2005 |
IN |
denotes |
from |
T11991 |
2006-2011 |
NNP |
denotes |
Peter |
T11992 |
2012-2020 |
NNP |
denotes |
Warnecke |
T11993 |
2021-2024 |
CC |
denotes |
and |
T11994 |
2025-2028 |
NNP |
denotes |
Tim |
T11995 |
2029-2035 |
NNP |
denotes |
Bestor |
T11996 |
2035-2036 |
. |
denotes |
. |
T11997 |
2036-2091 |
sentence |
denotes |
The PCR product included the entire 5′ LTR of the IAP. |
T11998 |
2037-2040 |
DT |
denotes |
The |
T11999 |
2045-2052 |
NN |
denotes |
product |
T12000 |
2041-2044 |
NN |
denotes |
PCR |
T12001 |
2053-2061 |
VBD |
denotes |
included |
T12002 |
2062-2065 |
DT |
denotes |
the |
T12003 |
2076-2079 |
NN |
denotes |
LTR |
T12004 |
2066-2072 |
JJ |
denotes |
entire |
T12005 |
2073-2074 |
CD |
denotes |
5 |
T12006 |
2074-2075 |
SYM |
denotes |
′ |
T12007 |
2080-2082 |
IN |
denotes |
of |
T12008 |
2083-2086 |
DT |
denotes |
the |
T12009 |
2087-2090 |
NN |
denotes |
IAP |
T12010 |
2090-2091 |
. |
denotes |
. |
T12011 |
2091-2144 |
sentence |
denotes |
All oligonucleotide sequences are shown in Table S1. |
T12012 |
2092-2095 |
DT |
denotes |
All |
T12013 |
2112-2121 |
NNS |
denotes |
sequences |
T12014 |
2096-2111 |
NN |
denotes |
oligonucleotide |
T12015 |
2126-2131 |
VBN |
denotes |
shown |
T12016 |
2122-2125 |
VBP |
denotes |
are |
T12017 |
2132-2134 |
IN |
denotes |
in |
T12018 |
2135-2140 |
NN |
denotes |
Table |
T12019 |
2141-2143 |
NN |
denotes |
S1 |
T12020 |
2143-2144 |
. |
denotes |
. |
R7147 |
T11583 |
T11584 |
compound |
ES,cell |
R7148 |
T11584 |
T11585 |
compound |
cell,clones |
R7149 |
T11585 |
T11586 |
nsubjpass |
clones,transfected |
R7150 |
T11587 |
T11585 |
acl |
bearing,clones |
R7151 |
T11588 |
T11589 |
det |
the,allele |
R7152 |
T11589 |
T11587 |
dobj |
allele,bearing |
R7153 |
T11590 |
T11589 |
compound |
Atrx flox,allele |
R7154 |
T11591 |
T11592 |
punct |
(,cells |
R7155 |
T11592 |
T11585 |
parataxis |
cells,clones |
R7156 |
T11593 |
T11594 |
quantmod |
1,107 |
R7157 |
T11594 |
T11592 |
nummod |
107,cells |
R7158 |
T11595 |
T11594 |
punct |
×,107 |
R7159 |
T11596 |
T11592 |
punct |
),cells |
R7160 |
T11597 |
T11586 |
auxpass |
were,transfected |
R7161 |
T11598 |
T11586 |
advmod |
transiently,transfected |
R7162 |
T11599 |
T11586 |
prep |
with,transfected |
R7163 |
T11600 |
T11601 |
nummod |
50,μg |
R7164 |
T11601 |
T11599 |
pobj |
μg,with |
R7165 |
T11602 |
T11601 |
prep |
of,μg |
R7166 |
T11603 |
T11604 |
amod |
uncut,plasmid |
R7167 |
T11604 |
T11602 |
pobj |
plasmid,of |
R7168 |
T11605 |
T11606 |
compound |
Cre,expression |
R7169 |
T11606 |
T11604 |
compound |
expression,plasmid |
R7170 |
T11607 |
T11604 |
punct |
(,plasmid |
R7171 |
T11608 |
T11609 |
compound |
pCAGGS,IRESpuro |
R7172 |
T11609 |
T11604 |
appos |
IRESpuro,plasmid |
R7173 |
T11610 |
T11609 |
punct |
-,IRESpuro |
R7174 |
T11611 |
T11609 |
compound |
Cre,IRESpuro |
R7175 |
T11612 |
T11609 |
punct |
-,IRESpuro |
R7176 |
T11613 |
T11586 |
punct |
),transfected |
R7177 |
T11614 |
T11615 |
punct |
[,40 |
R7178 |
T11615 |
T11586 |
parataxis |
40,transfected |
R7179 |
T11616 |
T11615 |
punct |
],40 |
R7180 |
T11617 |
T11586 |
punct |
.,transfected |
R7181 |
T11619 |
T11620 |
prep |
Following,plated |
R7182 |
T11621 |
T11619 |
pobj |
transfection,Following |
R7183 |
T11622 |
T11620 |
punct |
", ",plated |
R7184 |
T11623 |
T11620 |
nsubjpass |
cells,plated |
R7185 |
T11624 |
T11620 |
auxpass |
were,plated |
R7186 |
T11625 |
T11620 |
prep |
at,plated |
R7187 |
T11626 |
T11627 |
det |
a,range |
R7188 |
T11627 |
T11625 |
pobj |
range,at |
R7189 |
T11628 |
T11627 |
prep |
of,range |
R7190 |
T11629 |
T11630 |
amod |
clonal,densities |
R7191 |
T11630 |
T11628 |
pobj |
densities,of |
R7192 |
T11631 |
T11620 |
prep |
in,plated |
R7193 |
T11632 |
T11633 |
amod |
complete,medium |
R7194 |
T11633 |
T11631 |
pobj |
medium,in |
R7195 |
T11634 |
T11633 |
prep |
without,medium |
R7196 |
T11635 |
T11634 |
pobj |
G418,without |
R7197 |
T11636 |
T11620 |
punct |
", ",plated |
R7198 |
T11637 |
T11620 |
cc |
and,plated |
R7199 |
T11638 |
T11639 |
amod |
isolated,subclones |
R7200 |
T11639 |
T11640 |
nsubjpass |
subclones,picked |
R7201 |
T11640 |
T11620 |
conj |
picked,plated |
R7202 |
T11641 |
T11640 |
auxpass |
were,picked |
R7203 |
T11642 |
T11640 |
prep |
after,picked |
R7204 |
T11643 |
T11644 |
nummod |
7,d |
R7205 |
T11644 |
T11642 |
pobj |
d,after |
R7206 |
T11645 |
T11620 |
punct |
.,plated |
R7207 |
T11647 |
T11648 |
nsubjpass |
Subclones,expanded |
R7208 |
T11649 |
T11648 |
auxpass |
were,expanded |
R7209 |
T11650 |
T11648 |
cc |
and,expanded |
R7210 |
T11651 |
T11648 |
conj |
analysed,expanded |
R7211 |
T11652 |
T11651 |
prep |
for,analysed |
R7212 |
T11653 |
T11654 |
det |
the,presence |
R7213 |
T11654 |
T11652 |
pobj |
presence,for |
R7214 |
T11655 |
T11654 |
prep |
of,presence |
R7215 |
T11656 |
T11657 |
det |
a,locus |
R7216 |
T11657 |
T11655 |
pobj |
locus,of |
R7217 |
T11658 |
T11657 |
amod |
recombinant,locus |
R7218 |
T11659 |
T11660 |
advmod |
initially,by |
R7219 |
T11660 |
T11651 |
prep |
by,analysed |
R7220 |
T11661 |
T11660 |
pobj |
PCR,by |
R7221 |
T11662 |
T11660 |
punct |
", ",by |
R7222 |
T11663 |
T11664 |
aux |
to,detect |
R7223 |
T11664 |
T11660 |
advcl |
detect,by |
R7224 |
T11665 |
T11664 |
dobj |
deletion,detect |
R7225 |
T11666 |
T11665 |
prep |
of,deletion |
R7226 |
T11667 |
T11668 |
det |
the,cassette |
R7227 |
T11668 |
T11666 |
pobj |
cassette,of |
R7228 |
T11669 |
T11668 |
compound |
MC1neopA,cassette |
R7229 |
T11670 |
T11660 |
punct |
", ",by |
R7230 |
T11671 |
T11660 |
cc |
and,by |
R7231 |
T11672 |
T11673 |
advmod |
then,by |
R7232 |
T11673 |
T11660 |
conj |
by,by |
R7233 |
T11674 |
T11675 |
compound |
Southern,blot |
R7234 |
T11675 |
T11673 |
pobj |
blot,by |
R7235 |
T11676 |
T11675 |
cc |
and,blot |
R7236 |
T11677 |
T11675 |
conj |
hybridisation,blot |
R7237 |
T11678 |
T11675 |
prep |
with,blot |
R7238 |
T11679 |
T11680 |
det |
the,probe |
R7239 |
T11680 |
T11678 |
pobj |
probe,with |
R7240 |
T11681 |
T11680 |
nmod |
intron,probe |
R7241 |
T11682 |
T11681 |
nummod |
17,intron |
R7242 |
T11683 |
T11680 |
acl |
described,probe |
R7243 |
T11684 |
T11683 |
advmod |
above,described |
R7244 |
T11685 |
T11686 |
punct |
(,2A |
R7245 |
T11686 |
T11651 |
parataxis |
2A,analysed |
R7246 |
T11687 |
T11686 |
compound |
Figure,2A |
R7247 |
T11688 |
T11686 |
cc |
and,2A |
R7248 |
T11689 |
T11686 |
conj |
2C,2A |
R7249 |
T11690 |
T11686 |
punct |
),2A |
R7250 |
T11691 |
T11648 |
punct |
.,expanded |
R7251 |
T11693 |
T11694 |
compound |
Northern,blots |
R7252 |
T11694 |
T11695 |
nsubjpass |
blots,carried |
R7253 |
T11696 |
T11695 |
auxpass |
were,carried |
R7254 |
T11697 |
T11695 |
prt |
out,carried |
R7255 |
T11698 |
T11695 |
prep |
according,carried |
R7256 |
T11699 |
T11698 |
prep |
to,according |
R7257 |
T11700 |
T11701 |
amod |
standard,techniques |
R7258 |
T11701 |
T11699 |
pobj |
techniques,to |
R7259 |
T11702 |
T11695 |
advcl |
using,carried |
R7260 |
T11703 |
T11704 |
nummod |
20,μg |
R7261 |
T11704 |
T11702 |
dobj |
μg,using |
R7262 |
T11705 |
T11704 |
prep |
of,μg |
R7263 |
T11706 |
T11707 |
amod |
total,RNA |
R7264 |
T11707 |
T11705 |
pobj |
RNA,of |
R7265 |
T11708 |
T11707 |
acl |
isolated,RNA |
R7266 |
T11709 |
T11708 |
advcl |
using,isolated |
R7267 |
T11710 |
T11711 |
compound |
TRI,Reagent |
R7268 |
T11711 |
T11709 |
dobj |
Reagent,using |
R7269 |
T11712 |
T11713 |
punct |
(,Aldrich |
R7270 |
T11713 |
T11711 |
parataxis |
Aldrich,Reagent |
R7271 |
T11714 |
T11713 |
compound |
Sigma,Aldrich |
R7272 |
T11715 |
T11713 |
punct |
-,Aldrich |
R7273 |
T11716 |
T11713 |
punct |
", ",Aldrich |
R7274 |
T11717 |
T11718 |
compound |
St.,Louis |
R7275 |
T11718 |
T11713 |
npadvmod |
Louis,Aldrich |
R7276 |
T11719 |
T11713 |
punct |
", ",Aldrich |
R7277 |
T11720 |
T11713 |
npadvmod |
Missouri,Aldrich |
R7278 |
T11721 |
T11713 |
punct |
", ",Aldrich |
R7279 |
T11722 |
T11723 |
compound |
United,States |
R7280 |
T11723 |
T11713 |
npadvmod |
States,Aldrich |
R7281 |
T11724 |
T11713 |
punct |
),Aldrich |
R7282 |
T11725 |
T11695 |
punct |
.,carried |
R7283 |
T11727 |
T11728 |
det |
The,blot |
R7284 |
T11728 |
T11729 |
nsubjpass |
blot,hybridised |
R7285 |
T11730 |
T11729 |
auxpass |
was,hybridised |
R7286 |
T11731 |
T11729 |
prep |
with,hybridised |
R7287 |
T11732 |
T11733 |
det |
a,probe |
R7288 |
T11733 |
T11731 |
pobj |
probe,with |
R7289 |
T11734 |
T11733 |
prep |
from,probe |
R7290 |
T11735 |
T11734 |
prep |
within,from |
R7291 |
T11736 |
T11735 |
pobj |
exon,within |
R7292 |
T11737 |
T11736 |
nummod |
10,exon |
R7293 |
T11738 |
T11736 |
prep |
of,exon |
R7294 |
T11739 |
T11740 |
det |
the,gene |
R7295 |
T11740 |
T11738 |
pobj |
gene,of |
R7296 |
T11741 |
T11740 |
compound |
Atrx,gene |
R7297 |
T11742 |
T11733 |
punct |
(,probe |
R7298 |
T11743 |
T11733 |
acl |
generated,probe |
R7299 |
T11744 |
T11743 |
prep |
with,generated |
R7300 |
T11745 |
T11746 |
compound |
primers,Mxnp4 |
R7301 |
T11746 |
T11744 |
pobj |
Mxnp4,with |
R7302 |
T11747 |
T11746 |
cc |
and,Mxnp4 |
R7303 |
T11748 |
T11746 |
conj |
Mxnp28,Mxnp4 |
R7304 |
T11749 |
T11750 |
punct |
[,S1 |
R7305 |
T11750 |
T11729 |
parataxis |
S1,hybridised |
R7306 |
T11751 |
T11750 |
compound |
Table,S1 |
R7307 |
T11752 |
T11750 |
punct |
],S1 |
R7308 |
T11753 |
T11729 |
punct |
),hybridised |
R7309 |
T11754 |
T11729 |
punct |
.,hybridised |
R7310 |
T11756 |
T11757 |
mark |
After,stripped |
R7311 |
T11757 |
T11760 |
advcl |
stripped,hybridised |
R7312 |
T11758 |
T11757 |
nsubjpass |
it,stripped |
R7313 |
T11759 |
T11757 |
auxpass |
was,stripped |
R7314 |
T11761 |
T11760 |
punct |
", ",hybridised |
R7315 |
T11762 |
T11763 |
det |
the,membrane |
R7316 |
T11763 |
T11760 |
nsubjpass |
membrane,hybridised |
R7317 |
T11764 |
T11760 |
auxpass |
was,hybridised |
R7318 |
T11765 |
T11760 |
prep |
with,hybridised |
R7319 |
T11766 |
T11767 |
det |
a,probe |
R7320 |
T11767 |
T11765 |
pobj |
probe,with |
R7321 |
T11768 |
T11769 |
compound |
β,actin |
R7322 |
T11769 |
T11767 |
compound |
actin,probe |
R7323 |
T11770 |
T11769 |
punct |
-,actin |
R7324 |
T11771 |
T11767 |
compound |
cDNA,probe |
R7325 |
T11772 |
T11773 |
punct |
(,Clontech |
R7326 |
T11773 |
T11767 |
parataxis |
Clontech,probe |
R7327 |
T11774 |
T11773 |
punct |
", ",Clontech |
R7328 |
T11775 |
T11776 |
compound |
Palo,Alto |
R7329 |
T11776 |
T11773 |
npadvmod |
Alto,Clontech |
R7330 |
T11777 |
T11773 |
punct |
", ",Clontech |
R7331 |
T11778 |
T11773 |
npadvmod |
California,Clontech |
R7332 |
T11779 |
T11773 |
punct |
", ",Clontech |
R7333 |
T11780 |
T11781 |
compound |
United,States |
R7334 |
T11781 |
T11773 |
npadvmod |
States,Clontech |
R7335 |
T11782 |
T11773 |
punct |
),Clontech |
R7336 |
T11783 |
T11760 |
punct |
.,hybridised |
R7337 |
T11785 |
T11786 |
compound |
Protein,extraction |
R7338 |
T11786 |
T11787 |
nsubjpass |
extraction,performed |
R7339 |
T11788 |
T11786 |
cc |
and,extraction |
R7340 |
T11789 |
T11786 |
conj |
detection,extraction |
R7341 |
T11790 |
T11789 |
prep |
of,detection |
R7342 |
T11791 |
T11790 |
pobj |
Atrx,of |
R7343 |
T11792 |
T11789 |
prep |
by,detection |
R7344 |
T11793 |
T11794 |
compound |
Western,blotting |
R7345 |
T11794 |
T11792 |
pobj |
blotting,by |
R7346 |
T11795 |
T11787 |
auxpass |
was,performed |
R7347 |
T11796 |
T11797 |
mark |
as,described |
R7348 |
T11797 |
T11787 |
advcl |
described,performed |
R7349 |
T11798 |
T11797 |
advmod |
previously,described |
R7350 |
T11799 |
T11800 |
punct |
[,4 |
R7351 |
T11800 |
T11797 |
parataxis |
4,described |
R7352 |
T11801 |
T11800 |
punct |
],4 |
R7353 |
T11802 |
T11787 |
punct |
", ",performed |
R7354 |
T11803 |
T11787 |
advcl |
using,performed |
R7355 |
T11804 |
T11805 |
det |
the,antibody |
R7356 |
T11805 |
T11803 |
dobj |
antibody,using |
R7357 |
T11806 |
T11805 |
nmod |
mouse,antibody |
R7358 |
T11807 |
T11805 |
amod |
monoclonal,antibody |
R7359 |
T11808 |
T11805 |
amod |
anti-ATRX,antibody |
R7360 |
T11809 |
T11805 |
appos |
23C,antibody |
R7361 |
T11810 |
T11811 |
punct |
[,15 |
R7362 |
T11811 |
T11787 |
parataxis |
15,performed |
R7363 |
T11812 |
T11811 |
punct |
],15 |
R7364 |
T11813 |
T11787 |
punct |
.,performed |
R7365 |
T11815 |
T11816 |
nsubjpass |
Analyses,described |
R7366 |
T11817 |
T11815 |
prep |
of,Analyses |
R7367 |
T11818 |
T11819 |
compound |
cell,cycle |
R7368 |
T11819 |
T11817 |
pobj |
cycle,of |
R7369 |
T11820 |
T11819 |
cc |
and,cycle |
R7370 |
T11821 |
T11819 |
conj |
apoptosis,cycle |
R7371 |
T11822 |
T11816 |
auxpass |
are,described |
R7372 |
T11823 |
T11816 |
prep |
in,described |
R7373 |
T11824 |
T11825 |
compound |
Protocol,S1 |
R7374 |
T11825 |
T11823 |
pobj |
S1,in |
R7375 |
T11826 |
T11816 |
punct |
.,described |
R7376 |
T11828 |
T11829 |
nsubjpass |
Methylation,analysed |
R7377 |
T11830 |
T11828 |
prep |
of,Methylation |
R7378 |
T11831 |
T11830 |
pobj |
rDNA,of |
R7379 |
T11832 |
T11829 |
auxpass |
was,analysed |
R7380 |
T11833 |
T11829 |
prep |
in,analysed |
R7381 |
T11834 |
T11833 |
pobj |
DNA,in |
R7382 |
T11835 |
T11834 |
prep |
from,DNA |
R7383 |
T11836 |
T11837 |
compound |
ES,cell |
R7384 |
T11837 |
T11838 |
compound |
cell,clones |
R7385 |
T11838 |
T11835 |
pobj |
clones,from |
R7386 |
T11839 |
T11835 |
cc |
or,from |
R7387 |
T11840 |
T11835 |
conj |
from,from |
R7388 |
T11841 |
T11842 |
amod |
embryoid,bodies |
R7389 |
T11842 |
T11840 |
pobj |
bodies,from |
R7390 |
T11843 |
T11842 |
acl |
recovered,bodies |
R7391 |
T11844 |
T11843 |
prep |
after,recovered |
R7392 |
T11845 |
T11846 |
nummod |
7,d |
R7393 |
T11846 |
T11844 |
pobj |
d,after |
R7394 |
T11847 |
T11846 |
prep |
of,d |
R7395 |
T11848 |
T11849 |
advmod |
in,vitro |
R7396 |
T11849 |
T11850 |
amod |
vitro,differentiation |
R7397 |
T11850 |
T11847 |
pobj |
differentiation,of |
R7398 |
T11851 |
T11852 |
mark |
as,described |
R7399 |
T11852 |
T11829 |
advcl |
described,analysed |
R7400 |
T11853 |
T11852 |
advmod |
previously,described |
R7401 |
T11854 |
T11855 |
punct |
[,41 |
R7402 |
T11855 |
T11829 |
parataxis |
41,analysed |
R7403 |
T11856 |
T11855 |
punct |
],41 |
R7404 |
T11857 |
T11829 |
punct |
.,analysed |
R7405 |
T11859 |
T11860 |
amod |
Genomic,DNA |
R7406 |
T11860 |
T11861 |
nsubjpass |
DNA,digested |
R7407 |
T11862 |
T11861 |
auxpass |
was,digested |
R7408 |
T11863 |
T11861 |
prep |
with,digested |
R7409 |
T11864 |
T11865 |
npadvmod |
methylation,sensitive |
R7410 |
T11865 |
T11867 |
amod |
sensitive,enzymes |
R7411 |
T11866 |
T11865 |
punct |
-,sensitive |
R7412 |
T11867 |
T11863 |
pobj |
enzymes,with |
R7413 |
T11868 |
T11867 |
compound |
restriction,enzymes |
R7414 |
T11869 |
T11870 |
mark |
as,described |
R7415 |
T11870 |
T11861 |
advcl |
described,digested |
R7416 |
T11871 |
T11861 |
cc |
and,digested |
R7417 |
T11872 |
T11861 |
conj |
analysed,digested |
R7418 |
T11873 |
T11872 |
prep |
by,analysed |
R7419 |
T11874 |
T11875 |
compound |
Southern,blotting |
R7420 |
T11875 |
T11873 |
pobj |
blotting,by |
R7421 |
T11876 |
T11861 |
punct |
.,digested |
R7422 |
T11878 |
T11879 |
det |
The,probes |
R7423 |
T11879 |
T11883 |
nsubjpass |
probes,described |
R7424 |
T11880 |
T11879 |
nmod |
RIB3,probes |
R7425 |
T11881 |
T11880 |
cc |
and,RIB3 |
R7426 |
T11882 |
T11880 |
conj |
RIB4,RIB3 |
R7427 |
T11884 |
T11879 |
punct |
(,probes |
R7428 |
T11885 |
T11886 |
dep |
which,amplified |
R7429 |
T11886 |
T11879 |
relcl |
amplified,probes |
R7430 |
T11887 |
T11886 |
auxpass |
were,amplified |
R7431 |
T11888 |
T11886 |
prep |
from,amplified |
R7432 |
T11889 |
T11890 |
amod |
human,DNA |
R7433 |
T11890 |
T11888 |
pobj |
DNA,from |
R7434 |
T11891 |
T11886 |
punct |
", ",amplified |
R7435 |
T11892 |
T11886 |
cc |
but,amplified |
R7436 |
T11893 |
T11886 |
conj |
cross-react,amplified |
R7437 |
T11894 |
T11893 |
prep |
with,cross-react |
R7438 |
T11895 |
T11896 |
det |
the,repeat |
R7439 |
T11896 |
T11894 |
pobj |
repeat,with |
R7440 |
T11897 |
T11898 |
compound |
mouse,rDNA |
R7441 |
T11898 |
T11896 |
compound |
rDNA,repeat |
R7442 |
T11899 |
T11879 |
punct |
),probes |
R7443 |
T11900 |
T11883 |
aux |
have,described |
R7444 |
T11901 |
T11883 |
auxpass |
been,described |
R7445 |
T11902 |
T11883 |
advmod |
previously,described |
R7446 |
T11903 |
T11904 |
punct |
[,11 |
R7447 |
T11904 |
T11883 |
parataxis |
11,described |
R7448 |
T11905 |
T11904 |
punct |
],11 |
R7449 |
T11906 |
T11883 |
punct |
.,described |
R7450 |
T11908 |
T11909 |
compound |
Oligonucleotide,probes |
R7451 |
T11909 |
T11910 |
nsubjpass |
probes,described |
R7452 |
T11911 |
T11912 |
aux |
to,detect |
R7453 |
T11912 |
T11909 |
advcl |
detect,probes |
R7454 |
T11913 |
T11914 |
nmod |
Line,repeats |
R7455 |
T11914 |
T11912 |
dobj |
repeats,detect |
R7456 |
T11915 |
T11913 |
nummod |
1,Line |
R7457 |
T11916 |
T11913 |
cc |
and,Line |
R7458 |
T11917 |
T11918 |
compound |
Sine,B1 |
R7459 |
T11918 |
T11913 |
conj |
B1,Line |
R7460 |
T11919 |
T11910 |
aux |
have,described |
R7461 |
T11920 |
T11910 |
auxpass |
been,described |
R7462 |
T11921 |
T11910 |
advmod |
previously,described |
R7463 |
T11922 |
T11923 |
punct |
[,42 |
R7464 |
T11923 |
T11910 |
parataxis |
42,described |
R7465 |
T11924 |
T11923 |
punct |
],42 |
R7466 |
T11925 |
T11910 |
punct |
.,described |
R7467 |
T11927 |
T11928 |
det |
The,probe |
R7468 |
T11928 |
T11931 |
nsubj |
probe,was |
R7469 |
T11929 |
T11930 |
amod |
minor,satellite |
R7470 |
T11930 |
T11928 |
compound |
satellite,probe |
R7471 |
T11932 |
T11933 |
det |
a,oligonucleotide |
R7472 |
T11933 |
T11931 |
attr |
oligonucleotide,was |
R7473 |
T11934 |
T11935 |
nummod |
27,mer |
R7474 |
T11935 |
T11933 |
compound |
mer,oligonucleotide |
R7475 |
T11936 |
T11935 |
punct |
-,mer |
R7476 |
T11937 |
T11933 |
punct |
(,oligonucleotide |
R7477 |
T11938 |
T11933 |
appos |
mCENT2,oligonucleotide |
R7478 |
T11939 |
T11931 |
punct |
),was |
R7479 |
T11940 |
T11931 |
punct |
.,was |
R7480 |
T11942 |
T11943 |
det |
The,probe |
R7481 |
T11943 |
T11946 |
nsubj |
probe,was |
R7482 |
T11944 |
T11945 |
amod |
major,satellite |
R7483 |
T11945 |
T11943 |
compound |
satellite,probe |
R7484 |
T11947 |
T11948 |
det |
a,oligonucleotide |
R7485 |
T11948 |
T11946 |
attr |
oligonucleotide,was |
R7486 |
T11949 |
T11950 |
nummod |
27,mer |
R7487 |
T11950 |
T11948 |
compound |
mer,oligonucleotide |
R7488 |
T11951 |
T11950 |
punct |
-,mer |
R7489 |
T11952 |
T11948 |
punct |
(,oligonucleotide |
R7490 |
T11953 |
T11948 |
appos |
DG27,oligonucleotide |
R7491 |
T11954 |
T11946 |
punct |
),was |
R7492 |
T11955 |
T11946 |
punct |
.,was |
R7493 |
T11957 |
T11958 |
det |
The,probe |
R7494 |
T11958 |
T11960 |
nsubj |
probe,was |
R7495 |
T11959 |
T11958 |
compound |
IAP,probe |
R7496 |
T11961 |
T11962 |
det |
an,product |
R7497 |
T11962 |
T11960 |
attr |
product,was |
R7498 |
T11963 |
T11964 |
punct |
~,400 |
R7499 |
T11964 |
T11965 |
nummod |
400,bp |
R7500 |
T11965 |
T11966 |
compound |
bp,PCR |
R7501 |
T11966 |
T11962 |
compound |
PCR,product |
R7502 |
T11967 |
T11962 |
punct |
(,product |
R7503 |
T11968 |
T11969 |
compound |
primers,14A |
R7504 |
T11969 |
T11962 |
appos |
14A,product |
R7505 |
T11970 |
T11969 |
cc |
and,14A |
R7506 |
T11971 |
T11969 |
conj |
13K,14A |
R7507 |
T11972 |
T11962 |
punct |
),product |
R7508 |
T11973 |
T11962 |
acl |
amplified,product |
R7509 |
T11974 |
T11973 |
prep |
from,amplified |
R7510 |
T11975 |
T11976 |
det |
an,IAP |
R7511 |
T11976 |
T11974 |
pobj |
IAP,from |
R7512 |
T11977 |
T11976 |
acl |
inserted,IAP |
R7513 |
T11978 |
T11977 |
prep |
into,inserted |
R7514 |
T11979 |
T11980 |
det |
the,gene |
R7515 |
T11980 |
T11978 |
pobj |
gene,into |
R7516 |
T11981 |
T11982 |
compound |
mouse,agouti |
R7517 |
T11982 |
T11980 |
compound |
agouti,gene |
R7518 |
T11983 |
T11984 |
punct |
[,43 |
R7519 |
T11984 |
T11960 |
parataxis |
43,was |
R7520 |
T11985 |
T11984 |
punct |
],43 |
R7521 |
T11986 |
T11960 |
cc |
and,was |
R7522 |
T11987 |
T11960 |
conj |
was,was |
R7523 |
T11988 |
T11989 |
det |
a,gift |
R7524 |
T11989 |
T11987 |
attr |
gift,was |
R7525 |
T11990 |
T11989 |
prep |
from,gift |
R7526 |
T11991 |
T11992 |
compound |
Peter,Warnecke |
R7527 |
T11992 |
T11990 |
pobj |
Warnecke,from |
R7528 |
T11993 |
T11992 |
cc |
and,Warnecke |
R7529 |
T11994 |
T11995 |
compound |
Tim,Bestor |
R7530 |
T11995 |
T11992 |
conj |
Bestor,Warnecke |
R7531 |
T11996 |
T11960 |
punct |
.,was |
R7532 |
T11998 |
T11999 |
det |
The,product |
R7533 |
T11999 |
T12001 |
nsubj |
product,included |
R7534 |
T12000 |
T11999 |
compound |
PCR,product |
R7535 |
T12002 |
T12003 |
det |
the,LTR |
R7536 |
T12003 |
T12001 |
dobj |
LTR,included |
R7537 |
T12004 |
T12003 |
amod |
entire,LTR |
R7538 |
T12005 |
T12003 |
nummod |
5,LTR |
R7539 |
T12006 |
T12005 |
punct |
′,5 |
R7540 |
T12007 |
T12003 |
prep |
of,LTR |
R7541 |
T12008 |
T12009 |
det |
the,IAP |
R7542 |
T12009 |
T12007 |
pobj |
IAP,of |
R7543 |
T12010 |
T12001 |
punct |
.,included |
R7544 |
T12012 |
T12013 |
det |
All,sequences |
R7545 |
T12013 |
T12015 |
nsubjpass |
sequences,shown |
R7546 |
T12014 |
T12013 |
compound |
oligonucleotide,sequences |
R7547 |
T12016 |
T12015 |
auxpass |
are,shown |
R7548 |
T12017 |
T12015 |
prep |
in,shown |
R7549 |
T12018 |
T12019 |
compound |
Table,S1 |
R7550 |
T12019 |
T12017 |
pobj |
S1,in |
R7551 |
T12020 |
T12015 |
punct |
.,shown |