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PMC:1440874 / 50138-51110 JSONTXT

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craft-sa-dev

Id Subject Object Predicate Lexical cue
T11199 0-7 RB denotes Briefly
T11200 51-57 VBZ denotes places
T11201 7-9 , denotes ,
T11202 9-12 DT denotes the
T11203 23-29 NN denotes vector
T11204 13-22 NN denotes targeting
T11205 30-31 -LRB- denotes (
T11206 31-36 VBN denotes shown
T11207 37-39 IN denotes in
T11208 40-46 NN denotes Figure
T11209 47-49 NN denotes 2A
T11210 49-50 -RRB- denotes )
T11211 58-59 DT denotes a
T11212 65-69 NN denotes site
T11213 60-64 NN denotes loxP
T11214 70-76 IN denotes within
T11215 77-83 NN denotes intron
T11216 84-86 CD denotes 18
T11217 87-90 CC denotes and
T11218 91-92 DT denotes a
T11219 125-133 NN denotes cassette
T11220 93-97 NN denotes loxP
T11221 98-105 VBN denotes flanked
T11222 97-98 HYPH denotes -
T11223 106-114 NN denotes MC1neopA
T11224 115-124 NN denotes selection
T11225 134-136 IN denotes in
T11226 137-143 NN denotes intron
T11227 144-146 CD denotes 17
T11228 147-149 IN denotes of
T11229 150-153 DT denotes the
T11230 159-163 NN denotes gene
T11231 154-158 NN denotes Atrx
T11232 163-164 . denotes .
T11233 164-235 sentence denotes A detailed description of the targeting construct is provided in [20].
T11234 165-166 DT denotes A
T11235 176-187 NN denotes description
T11236 167-175 VBN denotes detailed
T11237 218-226 VBN denotes provided
T11238 188-190 IN denotes of
T11239 191-194 DT denotes the
T11240 205-214 NN denotes construct
T11241 195-204 NN denotes targeting
T11242 215-217 VBZ denotes is
T11243 227-229 IN denotes in
T11244 230-231 -LRB- denotes [
T11245 231-233 CD denotes 20
T11246 233-234 -RRB- denotes ]
T11247 234-235 . denotes .
T11248 235-375 sentence denotes Linearised plasmid (150 μg) was electroporated into 1 × 108 E14Tg2a ES cells, and colonies resistant to G418 and ganciclovir were isolated.
T11249 236-246 VBN denotes Linearised
T11250 247-254 NN denotes plasmid
T11251 268-282 VBN denotes electroporated
T11252 255-256 -LRB- denotes (
T11253 260-262 NNS denotes μg
T11254 256-259 CD denotes 150
T11255 262-263 -RRB- denotes )
T11256 264-267 VBD denotes was
T11257 283-287 IN denotes into
T11258 288-289 CD denotes 1
T11259 292-295 CD denotes 108
T11260 290-291 SYM denotes ×
T11261 307-312 NNS denotes cells
T11262 296-303 NN denotes E14Tg2a
T11263 304-306 NN denotes ES
T11264 312-314 , denotes ,
T11265 314-317 CC denotes and
T11266 318-326 NNS denotes colonies
T11267 366-374 VBN denotes isolated
T11268 327-336 JJ denotes resistant
T11269 337-339 IN denotes to
T11270 340-344 NN denotes G418
T11271 345-348 CC denotes and
T11272 349-360 NN denotes ganciclovir
T11273 361-365 VBD denotes were
T11274 374-375 . denotes .
T11275 375-609 sentence denotes Homologous targeting events were identified by Southern blot of EcoRI-digested DNA and hybridisation with a 5′ probe (generated with primers PPS1.20 and PPS1.27) and a 3′ probe (a 0.9-kb HaeIII fragment) as shown in Figure 2A and 2B.
T11276 376-386 JJ denotes Homologous
T11277 397-403 NNS denotes events
T11278 387-396 NN denotes targeting
T11279 409-419 VBN denotes identified
T11280 404-408 VBD denotes were
T11281 420-422 IN denotes by
T11282 423-431 NNP denotes Southern
T11283 432-436 NN denotes blot
T11284 437-439 IN denotes of
T11285 440-445 NN denotes EcoRI
T11286 446-454 VBN denotes digested
T11287 445-446 HYPH denotes -
T11288 455-458 NN denotes DNA
T11289 459-462 CC denotes and
T11290 463-476 NN denotes hybridisation
T11291 477-481 IN denotes with
T11292 482-483 DT denotes a
T11293 487-492 NN denotes probe
T11294 484-485 CD denotes 5
T11295 485-486 SYM denotes ′
T11296 493-494 -LRB- denotes (
T11297 494-503 VBN denotes generated
T11298 504-508 IN denotes with
T11299 509-516 NNS denotes primers
T11300 517-524 NN denotes PPS1.20
T11301 525-528 CC denotes and
T11302 529-536 NN denotes PPS1.27
T11303 536-537 -RRB- denotes )
T11304 538-541 CC denotes and
T11305 542-543 DT denotes a
T11306 547-552 NN denotes probe
T11307 544-545 CD denotes 3
T11308 545-546 SYM denotes ′
T11309 553-554 -LRB- denotes (
T11310 554-555 DT denotes a
T11311 570-578 NN denotes fragment
T11312 556-559 CD denotes 0.9
T11313 560-562 NN denotes kb
T11314 559-560 HYPH denotes -
T11315 563-569 NN denotes HaeIII
T11316 578-579 -RRB- denotes )
T11317 580-582 IN denotes as
T11318 583-588 VBN denotes shown
T11319 589-591 IN denotes in
T11320 592-598 NN denotes Figure
T11321 599-601 NN denotes 2A
T11322 602-605 CC denotes and
T11323 606-608 NN denotes 2B
T11324 608-609 . denotes .
T11325 609-928 sentence denotes DNA from correctly targeted clones was also digested with SacI and analysed by Southern blot and hybridisation with a probe from within intron 17 (a PCR product generated with primers PPS1.15 and Xnp46) to confirm that the loxP site within intron 18 had been included within the crossed-over region (Figure 2A and 2C).
T11326 610-613 NN denotes DNA
T11327 654-662 VBN denotes digested
T11328 614-618 IN denotes from
T11329 619-628 RB denotes correctly
T11330 629-637 VBN denotes targeted
T11331 638-644 NNS denotes clones
T11332 645-648 VBD denotes was
T11333 649-653 RB denotes also
T11334 663-667 IN denotes with
T11335 668-672 NN denotes SacI
T11336 673-676 CC denotes and
T11337 677-685 VBN denotes analysed
T11338 686-688 IN denotes by
T11339 689-697 NNP denotes Southern
T11340 698-702 NN denotes blot
T11341 703-706 CC denotes and
T11342 707-720 NN denotes hybridisation
T11343 721-725 IN denotes with
T11344 726-727 DT denotes a
T11345 728-733 NN denotes probe
T11346 734-738 IN denotes from
T11347 739-745 IN denotes within
T11348 746-752 NN denotes intron
T11349 753-755 CD denotes 17
T11350 756-757 -LRB- denotes (
T11351 757-758 DT denotes a
T11352 763-770 NN denotes product
T11353 759-762 NN denotes PCR
T11354 771-780 VBN denotes generated
T11355 781-785 IN denotes with
T11356 786-793 NNS denotes primers
T11357 794-801 NN denotes PPS1.15
T11358 802-805 CC denotes and
T11359 806-811 NN denotes Xnp46
T11360 811-812 -RRB- denotes )
T11361 813-815 TO denotes to
T11362 816-823 VB denotes confirm
T11363 824-828 IN denotes that
T11364 869-877 VBN denotes included
T11365 829-832 DT denotes the
T11366 838-842 NN denotes site
T11367 833-837 NN denotes loxP
T11368 843-849 IN denotes within
T11369 850-856 NN denotes intron
T11370 857-859 CD denotes 18
T11371 860-863 VBD denotes had
T11372 864-868 VBN denotes been
T11373 878-884 IN denotes within
T11374 885-888 DT denotes the
T11375 902-908 NN denotes region
T11376 889-896 VBN denotes crossed
T11377 896-897 HYPH denotes -
T11378 897-901 RP denotes over
T11379 909-910 -LRB- denotes (
T11380 917-919 NN denotes 2A
T11381 910-916 NN denotes Figure
T11382 920-923 CC denotes and
T11383 924-926 NN denotes 2C
T11384 926-927 -RRB- denotes )
T11385 927-928 . denotes .
T11386 928-972 sentence denotes Sequences of primers are shown in Table S1.
T11387 929-938 NNS denotes Sequences
T11388 954-959 VBN denotes shown
T11389 939-941 IN denotes of
T11390 942-949 NNS denotes primers
T11391 950-953 VBP denotes are
T11392 960-962 IN denotes in
T11393 963-968 NN denotes Table
T11394 969-971 NN denotes S1
T11395 971-972 . denotes .
R6949 T11199 T11200 advmod Briefly,places
R6950 T11201 T11200 punct ", ",places
R6951 T11202 T11203 det the,vector
R6952 T11203 T11200 nsubj vector,places
R6953 T11204 T11203 compound targeting,vector
R6954 T11205 T11203 punct (,vector
R6955 T11206 T11203 acl shown,vector
R6956 T11207 T11206 prep in,shown
R6957 T11208 T11209 compound Figure,2A
R6958 T11209 T11207 pobj 2A,in
R6959 T11210 T11200 punct ),places
R6960 T11211 T11212 det a,site
R6961 T11212 T11200 dobj site,places
R6962 T11213 T11212 compound loxP,site
R6963 T11214 T11200 prep within,places
R6964 T11215 T11214 pobj intron,within
R6965 T11216 T11215 nummod 18,intron
R6966 T11217 T11200 cc and,places
R6967 T11218 T11219 det a,cassette
R6968 T11219 T11200 conj cassette,places
R6969 T11220 T11221 npadvmod loxP,flanked
R6970 T11221 T11219 amod flanked,cassette
R6971 T11222 T11221 punct -,flanked
R6972 T11223 T11224 compound MC1neopA,selection
R6973 T11224 T11219 compound selection,cassette
R6974 T11225 T11219 prep in,cassette
R6975 T11226 T11225 pobj intron,in
R6976 T11227 T11226 nummod 17,intron
R6977 T11228 T11226 prep of,intron
R6978 T11229 T11230 det the,gene
R6979 T11230 T11228 pobj gene,of
R6980 T11231 T11230 compound Atrx,gene
R6981 T11232 T11200 punct .,places
R6982 T11234 T11235 det A,description
R6983 T11235 T11237 nsubjpass description,provided
R6984 T11236 T11235 amod detailed,description
R6985 T11238 T11235 prep of,description
R6986 T11239 T11240 det the,construct
R6987 T11240 T11238 pobj construct,of
R6988 T11241 T11240 compound targeting,construct
R6989 T11242 T11237 auxpass is,provided
R6990 T11243 T11237 prep in,provided
R6991 T11244 T11243 punct [,in
R6992 T11245 T11243 pobj 20,in
R6993 T11246 T11237 punct ],provided
R6994 T11247 T11237 punct .,provided
R6995 T11249 T11250 amod Linearised,plasmid
R6996 T11250 T11251 nsubjpass plasmid,electroporated
R6997 T11252 T11253 punct (,μg
R6998 T11253 T11250 parataxis μg,plasmid
R6999 T11254 T11253 nummod 150,μg
R7000 T11255 T11253 punct ),μg
R7001 T11256 T11251 auxpass was,electroporated
R7002 T11257 T11251 prep into,electroporated
R7003 T11258 T11259 quantmod 1,108
R7004 T11259 T11261 nummod 108,cells
R7005 T11260 T11259 punct ×,108
R7006 T11261 T11257 pobj cells,into
R7007 T11262 T11261 compound E14Tg2a,cells
R7008 T11263 T11261 compound ES,cells
R7009 T11264 T11251 punct ", ",electroporated
R7010 T11265 T11251 cc and,electroporated
R7011 T11266 T11267 nsubjpass colonies,isolated
R7012 T11267 T11251 conj isolated,electroporated
R7013 T11268 T11266 amod resistant,colonies
R7014 T11269 T11268 prep to,resistant
R7015 T11270 T11269 pobj G418,to
R7016 T11271 T11270 cc and,G418
R7017 T11272 T11270 conj ganciclovir,G418
R7018 T11273 T11267 auxpass were,isolated
R7019 T11274 T11267 punct .,isolated
R7020 T11276 T11277 amod Homologous,events
R7021 T11277 T11279 nsubjpass events,identified
R7022 T11278 T11277 compound targeting,events
R7023 T11280 T11279 auxpass were,identified
R7024 T11281 T11279 prep by,identified
R7025 T11282 T11283 compound Southern,blot
R7026 T11283 T11281 pobj blot,by
R7027 T11284 T11283 prep of,blot
R7028 T11285 T11286 npadvmod EcoRI,digested
R7029 T11286 T11288 amod digested,DNA
R7030 T11287 T11286 punct -,digested
R7031 T11288 T11284 pobj DNA,of
R7032 T11289 T11283 cc and,blot
R7033 T11290 T11283 conj hybridisation,blot
R7034 T11291 T11290 prep with,hybridisation
R7035 T11292 T11293 det a,probe
R7036 T11293 T11291 pobj probe,with
R7037 T11294 T11293 nummod 5,probe
R7038 T11295 T11294 punct ′,5
R7039 T11296 T11293 punct (,probe
R7040 T11297 T11293 acl generated,probe
R7041 T11298 T11297 prep with,generated
R7042 T11299 T11300 compound primers,PPS1.20
R7043 T11300 T11298 pobj PPS1.20,with
R7044 T11301 T11300 cc and,PPS1.20
R7045 T11302 T11300 conj PPS1.27,PPS1.20
R7046 T11303 T11293 punct ),probe
R7047 T11304 T11293 cc and,probe
R7048 T11305 T11306 det a,probe
R7049 T11306 T11293 conj probe,probe
R7050 T11307 T11306 nummod 3,probe
R7051 T11308 T11307 punct ′,3
R7052 T11309 T11306 punct (,probe
R7053 T11310 T11311 det a,fragment
R7054 T11311 T11306 appos fragment,probe
R7055 T11312 T11313 nummod 0.9,kb
R7056 T11313 T11311 compound kb,fragment
R7057 T11314 T11313 punct -,kb
R7058 T11315 T11311 compound HaeIII,fragment
R7059 T11316 T11279 punct ),identified
R7060 T11317 T11318 mark as,shown
R7061 T11318 T11279 advcl shown,identified
R7062 T11319 T11318 prep in,shown
R7063 T11320 T11321 compound Figure,2A
R7064 T11321 T11319 pobj 2A,in
R7065 T11322 T11321 cc and,2A
R7066 T11323 T11321 conj 2B,2A
R7067 T11324 T11279 punct .,identified
R7068 T11326 T11327 nsubj DNA,digested
R7069 T11328 T11326 prep from,DNA
R7070 T11329 T11330 advmod correctly,targeted
R7071 T11330 T11331 amod targeted,clones
R7072 T11331 T11328 pobj clones,from
R7073 T11332 T11327 aux was,digested
R7074 T11333 T11327 advmod also,digested
R7075 T11334 T11327 prep with,digested
R7076 T11335 T11334 pobj SacI,with
R7077 T11336 T11327 cc and,digested
R7078 T11337 T11327 conj analysed,digested
R7079 T11338 T11337 prep by,analysed
R7080 T11339 T11340 compound Southern,blot
R7081 T11340 T11338 pobj blot,by
R7082 T11341 T11340 cc and,blot
R7083 T11342 T11340 conj hybridisation,blot
R7084 T11343 T11342 prep with,hybridisation
R7085 T11344 T11345 det a,probe
R7086 T11345 T11343 pobj probe,with
R7087 T11346 T11345 prep from,probe
R7088 T11347 T11346 prep within,from
R7089 T11348 T11347 pobj intron,within
R7090 T11349 T11348 nummod 17,intron
R7091 T11350 T11345 punct (,probe
R7092 T11351 T11352 det a,product
R7093 T11352 T11345 appos product,probe
R7094 T11353 T11352 compound PCR,product
R7095 T11354 T11352 acl generated,product
R7096 T11355 T11354 prep with,generated
R7097 T11356 T11357 compound primers,PPS1.15
R7098 T11357 T11355 pobj PPS1.15,with
R7099 T11358 T11357 cc and,PPS1.15
R7100 T11359 T11357 conj Xnp46,PPS1.15
R7101 T11360 T11327 punct ),digested
R7102 T11361 T11362 aux to,confirm
R7103 T11362 T11327 advcl confirm,digested
R7104 T11363 T11364 mark that,included
R7105 T11364 T11362 ccomp included,confirm
R7106 T11365 T11366 det the,site
R7107 T11366 T11364 nsubjpass site,included
R7108 T11367 T11366 compound loxP,site
R7109 T11368 T11366 prep within,site
R7110 T11369 T11368 pobj intron,within
R7111 T11370 T11369 nummod 18,intron
R7112 T11371 T11364 aux had,included
R7113 T11372 T11364 auxpass been,included
R7114 T11373 T11364 prep within,included
R7115 T11374 T11375 det the,region
R7116 T11375 T11373 pobj region,within
R7117 T11376 T11375 amod crossed,region
R7118 T11377 T11376 punct -,crossed
R7119 T11378 T11376 prt over,crossed
R7120 T11379 T11380 punct (,2A
R7121 T11380 T11327 parataxis 2A,digested
R7122 T11381 T11380 compound Figure,2A
R7123 T11382 T11380 cc and,2A
R7124 T11383 T11380 conj 2C,2A
R7125 T11384 T11380 punct ),2A
R7126 T11385 T11327 punct .,digested
R7127 T11387 T11388 nsubjpass Sequences,shown
R7128 T11389 T11387 prep of,Sequences
R7129 T11390 T11389 pobj primers,of
R7130 T11391 T11388 auxpass are,shown
R7131 T11392 T11388 prep in,shown
R7132 T11393 T11394 compound Table,S1
R7133 T11394 T11392 pobj S1,in
R7134 T11395 T11388 punct .,shown

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T11156 13-29 SO_EXT:0001644 denotes targeting vector
T11157 60-69 SO_EXT:0000346 denotes loxP site
T11158 77-83 SO_EXT:0000188 denotes intron
T11159 93-105 SO:0000359 denotes loxP-flanked
T11160 125-133 SO_EXT:0005853 denotes cassette
T11161 137-143 SO_EXT:0000188 denotes intron
T11162 154-158 PR_EXT:000004503 denotes Atrx
T11163 159-163 SO_EXT:0000704 denotes gene
T11164 205-214 SO_EXT:engineered_biological_sequence denotes construct
T11165 236-246 SO_EXT:sequence_linearization denotes Linearised
T11166 247-254 SO_EXT:0000155 denotes plasmid
T11167 304-312 CL:0002322 denotes ES cells
T11168 307-312 CL_GO_EXT:cell denotes cells
T11169 340-344 CHEBI:42768 denotes G418
T11170 349-360 CHEBI:465284 denotes ganciclovir
T11171 455-458 CHEBI_SO_EXT:DNA denotes DNA
T11172 463-476 GO:0097617 denotes hybridisation
T11173 487-492 CHEBI_SO_EXT:molecular_probe denotes probe
T11174 509-516 SO_EXT:0000112 denotes primers
T11175 547-552 CHEBI_SO_EXT:molecular_probe denotes probe
T11176 561-562 CHEBI_SO_EXT:base denotes b
T11177 610-613 CHEBI_SO_EXT:DNA denotes DNA
T11178 638-644 SO_EXT:sequence_cloned_entity denotes clones
T11179 707-720 GO:0097617 denotes hybridisation
T11180 728-733 CHEBI_SO_EXT:molecular_probe denotes probe
T11181 746-752 SO_EXT:0000188 denotes intron
T11182 759-770 SO_EXT:0000006 denotes PCR product
T11183 786-793 SO_EXT:0000112 denotes primers
T11184 833-842 SO_EXT:0000346 denotes loxP site
T11185 850-856 SO_EXT:0000188 denotes intron
T11186 889-901 GO:0035825 denotes crossed-over
T11187 929-938 SO_EXT:biological_sequence denotes Sequences
T11188 942-949 SO_EXT:0000112 denotes primers

2_test

Id Subject Object Predicate Lexical cue
16628246-15668733-85799784 231-233 15668733 denotes 20
T64563 231-233 15668733 denotes 20

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T11127 13-29 SO:0001644 denotes targeting vector
T11128 60-69 SO:0000346 denotes loxP site
T11129 77-83 SO:0000188 denotes intron
T11130 93-105 SO:0000359 denotes loxP-flanked
T11131 125-133 SO:0005853 denotes cassette
T11132 137-143 SO:0000188 denotes intron
T11133 154-158 PR:000004503 denotes Atrx
T11134 159-163 SO:0000704 denotes gene
T11135 247-254 SO:0000155 denotes plasmid
T11136 304-312 CL:0002322 denotes ES cells
T11137 340-344 CHEBI:42768 denotes G418
T11138 349-360 CHEBI:465284 denotes ganciclovir
T11139 463-476 GO:0097617 denotes hybridisation
T11140 509-516 SO:0000112 denotes primers
T11141 707-720 GO:0097617 denotes hybridisation
T11142 746-752 SO:0000188 denotes intron
T11143 759-770 SO:0000006 denotes PCR product
T11144 786-793 SO:0000112 denotes primers
T11145 833-842 SO:0000346 denotes loxP site
T11146 850-856 SO:0000188 denotes intron
T11147 889-901 GO:0035825 denotes crossed-over
T11148 942-949 SO:0000112 denotes primers