Id |
Subject |
Object |
Predicate |
Lexical cue |
T3964 |
0-2 |
TO |
denotes |
To |
T3965 |
3-14 |
VB |
denotes |
investigate |
T3966 |
83-94 |
VBD |
denotes |
established |
T3967 |
15-18 |
DT |
denotes |
the |
T3968 |
19-23 |
NN |
denotes |
role |
T3969 |
24-26 |
IN |
denotes |
of |
T3970 |
27-30 |
DT |
denotes |
the |
T3971 |
36-43 |
NN |
denotes |
protein |
T3972 |
31-35 |
NN |
denotes |
Atrx |
T3973 |
44-50 |
IN |
denotes |
during |
T3974 |
51-56 |
NN |
denotes |
mouse |
T3975 |
57-68 |
NN |
denotes |
development |
T3976 |
68-70 |
, |
denotes |
, |
T3977 |
70-72 |
PRP |
denotes |
we |
T3978 |
73-82 |
RB |
denotes |
initially |
T3979 |
95-100 |
NNS |
denotes |
lines |
T3980 |
101-103 |
IN |
denotes |
of |
T3981 |
104-108 |
NNS |
denotes |
mice |
T3982 |
109-116 |
VBG |
denotes |
bearing |
T3983 |
117-120 |
DT |
denotes |
the |
T3984 |
131-137 |
NN |
denotes |
allele |
T3985 |
121-130 |
NN |
denotes |
Atrx flox |
T3986 |
137-138 |
. |
denotes |
. |
T3987 |
138-337 |
sentence |
denotes |
Two independently targeted Atrx flox ES cell clones with normal male karyotype were injected into C57BL/6 blastocysts to produce chimaeric mice, which were then used to obtain germline transmission. |
T3988 |
139-142 |
CD |
denotes |
Two |
T3989 |
184-190 |
NNS |
denotes |
clones |
T3990 |
143-156 |
RB |
denotes |
independently |
T3991 |
157-165 |
VBN |
denotes |
targeted |
T3992 |
166-175 |
NN |
denotes |
Atrx flox |
T3993 |
176-178 |
NN |
denotes |
ES |
T3994 |
179-183 |
NN |
denotes |
cell |
T3995 |
223-231 |
VBN |
denotes |
injected |
T3996 |
191-195 |
IN |
denotes |
with |
T3997 |
196-202 |
JJ |
denotes |
normal |
T3998 |
208-217 |
NN |
denotes |
karyotype |
T3999 |
203-207 |
JJ |
denotes |
male |
T4000 |
218-222 |
VBD |
denotes |
were |
T4001 |
232-236 |
IN |
denotes |
into |
T4002 |
237-242 |
NN |
denotes |
C57BL |
T4003 |
245-256 |
NNS |
denotes |
blastocysts |
T4004 |
242-243 |
HYPH |
denotes |
/ |
T4005 |
243-244 |
CD |
denotes |
6 |
T4006 |
257-259 |
TO |
denotes |
to |
T4007 |
260-267 |
VB |
denotes |
produce |
T4008 |
268-277 |
JJ |
denotes |
chimaeric |
T4009 |
278-282 |
NNS |
denotes |
mice |
T4010 |
282-284 |
, |
denotes |
, |
T4011 |
284-289 |
WDT |
denotes |
which |
T4012 |
300-304 |
VBN |
denotes |
used |
T4013 |
290-294 |
VBD |
denotes |
were |
T4014 |
295-299 |
RB |
denotes |
then |
T4015 |
305-307 |
TO |
denotes |
to |
T4016 |
308-314 |
VB |
denotes |
obtain |
T4017 |
315-323 |
NN |
denotes |
germline |
T4018 |
324-336 |
NN |
denotes |
transmission |
T4019 |
336-337 |
. |
denotes |
. |
T4020 |
337-522 |
sentence |
denotes |
Intercrosses between males hemizygous (Atrx flox/Y) and females heterozygous (Atrx WT/flox) for the floxed allele were also carried out to generate homozygous females (Atrx flox/flox). |
T4021 |
338-350 |
NNS |
denotes |
Intercrosses |
T4022 |
462-469 |
VBN |
denotes |
carried |
T4023 |
351-358 |
IN |
denotes |
between |
T4024 |
359-364 |
NNS |
denotes |
males |
T4025 |
365-375 |
JJ |
denotes |
hemizygous |
T4026 |
376-377 |
-LRB- |
denotes |
( |
T4027 |
387-388 |
NN |
denotes |
Y |
T4028 |
377-386 |
NN |
denotes |
Atrx flox |
T4029 |
386-387 |
HYPH |
denotes |
/ |
T4030 |
388-389 |
-RRB- |
denotes |
) |
T4031 |
390-393 |
CC |
denotes |
and |
T4032 |
394-401 |
NNS |
denotes |
females |
T4033 |
402-414 |
JJ |
denotes |
heterozygous |
T4034 |
415-416 |
-LRB- |
denotes |
( |
T4035 |
424-428 |
NN |
denotes |
flox |
T4036 |
416-423 |
NN |
denotes |
Atrx WT |
T4037 |
423-424 |
HYPH |
denotes |
/ |
T4038 |
428-429 |
-RRB- |
denotes |
) |
T4039 |
430-433 |
IN |
denotes |
for |
T4040 |
434-437 |
DT |
denotes |
the |
T4041 |
445-451 |
NN |
denotes |
allele |
T4042 |
438-444 |
VBN |
denotes |
floxed |
T4043 |
452-456 |
VBD |
denotes |
were |
T4044 |
457-461 |
RB |
denotes |
also |
T4045 |
470-473 |
RP |
denotes |
out |
T4046 |
474-476 |
TO |
denotes |
to |
T4047 |
477-485 |
VB |
denotes |
generate |
T4048 |
486-496 |
JJ |
denotes |
homozygous |
T4049 |
497-504 |
NNS |
denotes |
females |
T4050 |
505-506 |
-LRB- |
denotes |
( |
T4051 |
516-520 |
NN |
denotes |
flox |
T4052 |
506-515 |
NN |
denotes |
Atrx flox |
T4053 |
515-516 |
HYPH |
denotes |
/ |
T4054 |
520-521 |
-RRB- |
denotes |
) |
T4055 |
521-522 |
. |
denotes |
. |
T4056 |
522-729 |
sentence |
denotes |
Males hemizygous and females heterozygous or homozygous for the Atrx flox allele were viable, appeared healthy, and bred normally, suggesting that, as expected, the Atrx flox allele was functionally normal. |
T4057 |
523-528 |
NNS |
denotes |
Males |
T4058 |
604-608 |
VBD |
denotes |
were |
T4059 |
529-539 |
JJ |
denotes |
hemizygous |
T4060 |
540-543 |
CC |
denotes |
and |
T4061 |
544-551 |
NNS |
denotes |
females |
T4062 |
552-564 |
JJ |
denotes |
heterozygous |
T4063 |
565-567 |
CC |
denotes |
or |
T4064 |
568-578 |
JJ |
denotes |
homozygous |
T4065 |
579-582 |
IN |
denotes |
for |
T4066 |
583-586 |
DT |
denotes |
the |
T4067 |
597-603 |
NN |
denotes |
allele |
T4068 |
587-596 |
NN |
denotes |
Atrx flox |
T4069 |
609-615 |
JJ |
denotes |
viable |
T4070 |
615-617 |
, |
denotes |
, |
T4071 |
617-625 |
VBD |
denotes |
appeared |
T4072 |
626-633 |
JJ |
denotes |
healthy |
T4073 |
633-635 |
, |
denotes |
, |
T4074 |
635-638 |
CC |
denotes |
and |
T4075 |
639-643 |
VBD |
denotes |
bred |
T4076 |
644-652 |
RB |
denotes |
normally |
T4077 |
652-654 |
, |
denotes |
, |
T4078 |
654-664 |
VBG |
denotes |
suggesting |
T4079 |
665-669 |
IN |
denotes |
that |
T4080 |
705-708 |
VBD |
denotes |
was |
T4081 |
669-671 |
, |
denotes |
, |
T4082 |
671-673 |
IN |
denotes |
as |
T4083 |
674-682 |
VBN |
denotes |
expected |
T4084 |
682-684 |
, |
denotes |
, |
T4085 |
684-687 |
DT |
denotes |
the |
T4086 |
698-704 |
NN |
denotes |
allele |
T4087 |
688-697 |
NN |
denotes |
Atrx flox |
T4088 |
709-721 |
RB |
denotes |
functionally |
T4089 |
722-728 |
JJ |
denotes |
normal |
T4090 |
728-729 |
. |
denotes |
. |
T4091 |
729-980 |
sentence |
denotes |
To generate Atrxnull mice by Cre-mediated recombination, the Atrx flox mice were crossed with mice harboring a transgene in which the Cre recombinase is expressed under the control of the regulatory elements of the mouse GATA-1 gene (GATA1-cre) [14]. |
T4092 |
730-732 |
TO |
denotes |
To |
T4093 |
733-741 |
VB |
denotes |
generate |
T4094 |
811-818 |
VBN |
denotes |
crossed |
T4095 |
742-750 |
JJ |
denotes |
Atrxnull |
T4096 |
751-755 |
NNS |
denotes |
mice |
T4097 |
756-758 |
IN |
denotes |
by |
T4098 |
759-762 |
NN |
denotes |
Cre |
T4099 |
763-771 |
VBN |
denotes |
mediated |
T4100 |
762-763 |
HYPH |
denotes |
- |
T4101 |
772-785 |
NN |
denotes |
recombination |
T4102 |
785-787 |
, |
denotes |
, |
T4103 |
787-790 |
DT |
denotes |
the |
T4104 |
801-805 |
NNS |
denotes |
mice |
T4105 |
791-800 |
NN |
denotes |
Atrx flox |
T4106 |
806-810 |
VBD |
denotes |
were |
T4107 |
819-823 |
IN |
denotes |
with |
T4108 |
824-828 |
NNS |
denotes |
mice |
T4109 |
829-838 |
VBG |
denotes |
harboring |
T4110 |
839-840 |
DT |
denotes |
a |
T4111 |
841-850 |
NN |
denotes |
transgene |
T4112 |
851-853 |
IN |
denotes |
in |
T4113 |
883-892 |
VBN |
denotes |
expressed |
T4114 |
854-859 |
WDT |
denotes |
which |
T4115 |
860-863 |
DT |
denotes |
the |
T4116 |
868-879 |
NN |
denotes |
recombinase |
T4117 |
864-867 |
NN |
denotes |
Cre |
T4118 |
880-882 |
VBZ |
denotes |
is |
T4119 |
893-898 |
IN |
denotes |
under |
T4120 |
899-902 |
DT |
denotes |
the |
T4121 |
903-910 |
NN |
denotes |
control |
T4122 |
911-913 |
IN |
denotes |
of |
T4123 |
914-917 |
DT |
denotes |
the |
T4124 |
929-937 |
NNS |
denotes |
elements |
T4125 |
918-928 |
JJ |
denotes |
regulatory |
T4126 |
938-940 |
IN |
denotes |
of |
T4127 |
941-944 |
DT |
denotes |
the |
T4128 |
958-962 |
NN |
denotes |
gene |
T4129 |
945-950 |
NN |
denotes |
mouse |
T4130 |
951-955 |
NN |
denotes |
GATA |
T4131 |
955-956 |
HYPH |
denotes |
- |
T4132 |
956-957 |
CD |
denotes |
1 |
T4133 |
963-964 |
-LRB- |
denotes |
( |
T4134 |
964-969 |
NN |
denotes |
GATA1 |
T4135 |
970-973 |
NN |
denotes |
cre |
T4136 |
969-970 |
HYPH |
denotes |
- |
T4137 |
973-974 |
-RRB- |
denotes |
) |
T4138 |
975-976 |
-LRB- |
denotes |
[ |
T4139 |
976-978 |
CD |
denotes |
14 |
T4140 |
978-979 |
-RRB- |
denotes |
] |
T4141 |
979-980 |
. |
denotes |
. |
T4142 |
980-1084 |
sentence |
denotes |
Widespread expression of the GATA1-cre transgene has been demonstrated during early embryogenesis [14]. |
T4143 |
981-991 |
JJ |
denotes |
Widespread |
T4144 |
992-1002 |
NN |
denotes |
expression |
T4145 |
1039-1051 |
VBN |
denotes |
demonstrated |
T4146 |
1003-1005 |
IN |
denotes |
of |
T4147 |
1006-1009 |
DT |
denotes |
the |
T4148 |
1020-1029 |
NN |
denotes |
transgene |
T4149 |
1010-1015 |
NN |
denotes |
GATA1 |
T4150 |
1016-1019 |
NN |
denotes |
cre |
T4151 |
1015-1016 |
HYPH |
denotes |
- |
T4152 |
1030-1033 |
VBZ |
denotes |
has |
T4153 |
1034-1038 |
VBN |
denotes |
been |
T4154 |
1052-1058 |
IN |
denotes |
during |
T4155 |
1059-1064 |
JJ |
denotes |
early |
T4156 |
1065-1078 |
NN |
denotes |
embryogenesis |
T4157 |
1079-1080 |
-LRB- |
denotes |
[ |
T4158 |
1080-1082 |
CD |
denotes |
14 |
T4159 |
1082-1083 |
-RRB- |
denotes |
] |
T4160 |
1083-1084 |
. |
denotes |
. |
T4161 |
1084-1348 |
sentence |
denotes |
We more accurately defined the onset of GATA1-cre expression using a ROSA26 reporter strain, in which a β-galactosidase/neor fusion reporter gene is expressed only after Cre-mediated excision of loxP-flanked transcription and translation termination signals [14]. |
T4162 |
1085-1087 |
PRP |
denotes |
We |
T4163 |
1104-1111 |
VBN |
denotes |
defined |
T4164 |
1088-1092 |
RBR |
denotes |
more |
T4165 |
1093-1103 |
RB |
denotes |
accurately |
T4166 |
1112-1115 |
DT |
denotes |
the |
T4167 |
1116-1121 |
NN |
denotes |
onset |
T4168 |
1122-1124 |
IN |
denotes |
of |
T4169 |
1125-1130 |
NN |
denotes |
GATA1 |
T4170 |
1131-1134 |
NN |
denotes |
cre |
T4171 |
1130-1131 |
HYPH |
denotes |
- |
T4172 |
1135-1145 |
NN |
denotes |
expression |
T4173 |
1146-1151 |
VBG |
denotes |
using |
T4174 |
1152-1153 |
DT |
denotes |
a |
T4175 |
1170-1176 |
NN |
denotes |
strain |
T4176 |
1154-1160 |
NN |
denotes |
ROSA26 |
T4177 |
1161-1169 |
NN |
denotes |
reporter |
T4178 |
1176-1178 |
, |
denotes |
, |
T4179 |
1178-1180 |
IN |
denotes |
in |
T4180 |
1234-1243 |
VBN |
denotes |
expressed |
T4181 |
1181-1186 |
WDT |
denotes |
which |
T4182 |
1187-1188 |
DT |
denotes |
a |
T4183 |
1226-1230 |
NN |
denotes |
gene |
T4184 |
1189-1190 |
NN |
denotes |
β |
T4185 |
1191-1204 |
NN |
denotes |
galactosidase |
T4186 |
1190-1191 |
HYPH |
denotes |
- |
T4187 |
1210-1216 |
NN |
denotes |
fusion |
T4188 |
1204-1205 |
HYPH |
denotes |
/ |
T4189 |
1205-1209 |
NN |
denotes |
neor |
T4190 |
1217-1225 |
NN |
denotes |
reporter |
T4191 |
1231-1233 |
VBZ |
denotes |
is |
T4192 |
1244-1248 |
RB |
denotes |
only |
T4193 |
1249-1254 |
IN |
denotes |
after |
T4194 |
1255-1258 |
NN |
denotes |
Cre |
T4195 |
1259-1267 |
VBN |
denotes |
mediated |
T4196 |
1258-1259 |
HYPH |
denotes |
- |
T4197 |
1268-1276 |
NN |
denotes |
excision |
T4198 |
1277-1279 |
IN |
denotes |
of |
T4199 |
1280-1284 |
NN |
denotes |
loxP |
T4200 |
1285-1292 |
VBN |
denotes |
flanked |
T4201 |
1284-1285 |
HYPH |
denotes |
- |
T4202 |
1293-1306 |
NN |
denotes |
transcription |
T4203 |
1307-1310 |
CC |
denotes |
and |
T4204 |
1311-1322 |
NN |
denotes |
translation |
T4205 |
1323-1334 |
NN |
denotes |
termination |
T4206 |
1335-1342 |
NNS |
denotes |
signals |
T4207 |
1343-1344 |
-LRB- |
denotes |
[ |
T4208 |
1344-1346 |
CD |
denotes |
14 |
T4209 |
1346-1347 |
-RRB- |
denotes |
] |
T4210 |
1347-1348 |
. |
denotes |
. |
T4211 |
1348-1489 |
sentence |
denotes |
We found that the GATA1-cre transgene was already active at the 16-cell morula stage of development (0.5 days postcoitus [dpc]) (Figure 4A). |
T4212 |
1349-1351 |
PRP |
denotes |
We |
T4213 |
1352-1357 |
VBD |
denotes |
found |
T4214 |
1358-1362 |
IN |
denotes |
that |
T4215 |
1387-1390 |
VBD |
denotes |
was |
T4216 |
1363-1366 |
DT |
denotes |
the |
T4217 |
1377-1386 |
NN |
denotes |
transgene |
T4218 |
1367-1372 |
NN |
denotes |
GATA1 |
T4219 |
1373-1376 |
NN |
denotes |
cre |
T4220 |
1372-1373 |
HYPH |
denotes |
- |
T4221 |
1391-1398 |
RB |
denotes |
already |
T4222 |
1399-1405 |
JJ |
denotes |
active |
T4223 |
1406-1408 |
IN |
denotes |
at |
T4224 |
1409-1412 |
DT |
denotes |
the |
T4225 |
1428-1433 |
NN |
denotes |
stage |
T4226 |
1413-1415 |
CD |
denotes |
16 |
T4227 |
1416-1420 |
NN |
denotes |
cell |
T4228 |
1415-1416 |
HYPH |
denotes |
- |
T4229 |
1421-1427 |
NN |
denotes |
morula |
T4230 |
1434-1436 |
IN |
denotes |
of |
T4231 |
1437-1448 |
NN |
denotes |
development |
T4232 |
1449-1450 |
-LRB- |
denotes |
( |
T4233 |
1450-1453 |
CD |
denotes |
0.5 |
T4234 |
1454-1458 |
NNS |
denotes |
days |
T4235 |
1459-1469 |
RB |
denotes |
postcoitus |
T4236 |
1470-1471 |
-LRB- |
denotes |
[ |
T4237 |
1471-1474 |
NN |
denotes |
dpc |
T4238 |
1474-1475 |
-RRB- |
denotes |
] |
T4239 |
1475-1476 |
-RRB- |
denotes |
) |
T4240 |
1477-1478 |
-LRB- |
denotes |
( |
T4241 |
1485-1487 |
NN |
denotes |
4A |
T4242 |
1478-1484 |
NN |
denotes |
Figure |
T4243 |
1487-1488 |
-RRB- |
denotes |
) |
T4244 |
1488-1489 |
. |
denotes |
. |
R2111 |
T3964 |
T3965 |
aux |
To,investigate |
R2112 |
T3965 |
T3966 |
advcl |
investigate,established |
R2113 |
T3967 |
T3968 |
det |
the,role |
R2114 |
T3968 |
T3965 |
dobj |
role,investigate |
R2115 |
T3969 |
T3968 |
prep |
of,role |
R2116 |
T3970 |
T3971 |
det |
the,protein |
R2117 |
T3971 |
T3969 |
pobj |
protein,of |
R2118 |
T3972 |
T3971 |
compound |
Atrx,protein |
R2119 |
T3973 |
T3968 |
prep |
during,role |
R2120 |
T3974 |
T3975 |
compound |
mouse,development |
R2121 |
T3975 |
T3973 |
pobj |
development,during |
R2122 |
T3976 |
T3966 |
punct |
", ",established |
R2123 |
T3977 |
T3966 |
nsubj |
we,established |
R2124 |
T3978 |
T3966 |
advmod |
initially,established |
R2125 |
T3979 |
T3966 |
dobj |
lines,established |
R2126 |
T3980 |
T3979 |
prep |
of,lines |
R2127 |
T3981 |
T3980 |
pobj |
mice,of |
R2128 |
T3982 |
T3979 |
acl |
bearing,lines |
R2129 |
T3983 |
T3984 |
det |
the,allele |
R2130 |
T3984 |
T3982 |
dobj |
allele,bearing |
R2131 |
T3985 |
T3984 |
compound |
Atrx flox,allele |
R2132 |
T3986 |
T3966 |
punct |
.,established |
R2133 |
T3988 |
T3989 |
nummod |
Two,clones |
R2134 |
T3989 |
T3995 |
nsubjpass |
clones,injected |
R2135 |
T3990 |
T3991 |
advmod |
independently,targeted |
R2136 |
T3991 |
T3989 |
amod |
targeted,clones |
R2137 |
T3992 |
T3989 |
compound |
Atrx flox,clones |
R2138 |
T3993 |
T3994 |
compound |
ES,cell |
R2139 |
T3994 |
T3989 |
compound |
cell,clones |
R2140 |
T3996 |
T3989 |
prep |
with,clones |
R2141 |
T3997 |
T3998 |
amod |
normal,karyotype |
R2142 |
T3998 |
T3996 |
pobj |
karyotype,with |
R2143 |
T3999 |
T3998 |
amod |
male,karyotype |
R2144 |
T4000 |
T3995 |
auxpass |
were,injected |
R2145 |
T4001 |
T3995 |
prep |
into,injected |
R2146 |
T4002 |
T4003 |
nmod |
C57BL,blastocysts |
R2147 |
T4003 |
T4001 |
pobj |
blastocysts,into |
R2148 |
T4004 |
T4002 |
punct |
/,C57BL |
R2149 |
T4005 |
T4002 |
nummod |
6,C57BL |
R2150 |
T4006 |
T4007 |
aux |
to,produce |
R2151 |
T4007 |
T3995 |
advcl |
produce,injected |
R2152 |
T4008 |
T4009 |
amod |
chimaeric,mice |
R2153 |
T4009 |
T4007 |
dobj |
mice,produce |
R2154 |
T4010 |
T4009 |
punct |
", ",mice |
R2155 |
T4011 |
T4012 |
dep |
which,used |
R2156 |
T4012 |
T4009 |
relcl |
used,mice |
R2157 |
T4013 |
T4012 |
auxpass |
were,used |
R2158 |
T4014 |
T4012 |
advmod |
then,used |
R2159 |
T4015 |
T4016 |
aux |
to,obtain |
R2160 |
T4016 |
T4012 |
advcl |
obtain,used |
R2161 |
T4017 |
T4018 |
compound |
germline,transmission |
R2162 |
T4018 |
T4016 |
dobj |
transmission,obtain |
R2163 |
T4019 |
T3995 |
punct |
.,injected |
R2164 |
T4021 |
T4022 |
nsubjpass |
Intercrosses,carried |
R2165 |
T4023 |
T4021 |
prep |
between,Intercrosses |
R2166 |
T4024 |
T4023 |
pobj |
males,between |
R2167 |
T4025 |
T4024 |
amod |
hemizygous,males |
R2168 |
T4026 |
T4027 |
punct |
(,Y |
R2169 |
T4027 |
T4025 |
parataxis |
Y,hemizygous |
R2170 |
T4028 |
T4027 |
compound |
Atrx flox,Y |
R2171 |
T4029 |
T4027 |
punct |
/,Y |
R2172 |
T4030 |
T4027 |
punct |
),Y |
R2173 |
T4031 |
T4024 |
cc |
and,males |
R2174 |
T4032 |
T4024 |
conj |
females,males |
R2175 |
T4033 |
T4032 |
amod |
heterozygous,females |
R2176 |
T4034 |
T4035 |
punct |
(,flox |
R2177 |
T4035 |
T4033 |
parataxis |
flox,heterozygous |
R2178 |
T4036 |
T4035 |
compound |
Atrx WT,flox |
R2179 |
T4037 |
T4035 |
punct |
/,flox |
R2180 |
T4038 |
T4035 |
punct |
),flox |
R2181 |
T4039 |
T4032 |
prep |
for,females |
R2182 |
T4040 |
T4041 |
det |
the,allele |
R2183 |
T4041 |
T4039 |
pobj |
allele,for |
R2184 |
T4042 |
T4041 |
amod |
floxed,allele |
R2185 |
T4043 |
T4022 |
auxpass |
were,carried |
R2186 |
T4044 |
T4022 |
advmod |
also,carried |
R2187 |
T4045 |
T4022 |
prt |
out,carried |
R2188 |
T4046 |
T4047 |
aux |
to,generate |
R2189 |
T4047 |
T4022 |
advcl |
generate,carried |
R2190 |
T4048 |
T4049 |
amod |
homozygous,females |
R2191 |
T4049 |
T4047 |
dobj |
females,generate |
R2192 |
T4050 |
T4051 |
punct |
(,flox |
R2193 |
T4051 |
T4049 |
parataxis |
flox,females |
R2194 |
T4052 |
T4051 |
compound |
Atrx flox,flox |
R2195 |
T4053 |
T4051 |
punct |
/,flox |
R2196 |
T4054 |
T4051 |
punct |
),flox |
R2197 |
T4055 |
T4022 |
punct |
.,carried |
R2198 |
T4057 |
T4058 |
nsubj |
Males,were |
R2199 |
T4059 |
T4057 |
amod |
hemizygous,Males |
R2200 |
T4060 |
T4057 |
cc |
and,Males |
R2201 |
T4061 |
T4057 |
conj |
females,Males |
R2202 |
T4062 |
T4061 |
amod |
heterozygous,females |
R2203 |
T4063 |
T4062 |
cc |
or,heterozygous |
R2204 |
T4064 |
T4062 |
conj |
homozygous,heterozygous |
R2205 |
T4065 |
T4061 |
prep |
for,females |
R2206 |
T4066 |
T4067 |
det |
the,allele |
R2207 |
T4067 |
T4065 |
pobj |
allele,for |
R2208 |
T4068 |
T4067 |
compound |
Atrx flox,allele |
R2209 |
T4069 |
T4058 |
acomp |
viable,were |
R2210 |
T4070 |
T4058 |
punct |
", ",were |
R2211 |
T4071 |
T4058 |
conj |
appeared,were |
R2212 |
T4072 |
T4071 |
oprd |
healthy,appeared |
R2213 |
T4073 |
T4071 |
punct |
", ",appeared |
R2214 |
T4074 |
T4071 |
cc |
and,appeared |
R2215 |
T4075 |
T4071 |
conj |
bred,appeared |
R2216 |
T4076 |
T4075 |
advmod |
normally,bred |
R2217 |
T4077 |
T4058 |
punct |
", ",were |
R2218 |
T4078 |
T4058 |
advcl |
suggesting,were |
R2219 |
T4079 |
T4080 |
mark |
that,was |
R2220 |
T4080 |
T4078 |
ccomp |
was,suggesting |
R2221 |
T4081 |
T4080 |
punct |
", ",was |
R2222 |
T4082 |
T4083 |
mark |
as,expected |
R2223 |
T4083 |
T4080 |
advcl |
expected,was |
R2224 |
T4084 |
T4080 |
punct |
", ",was |
R2225 |
T4085 |
T4086 |
det |
the,allele |
R2226 |
T4086 |
T4080 |
nsubj |
allele,was |
R2227 |
T4087 |
T4086 |
compound |
Atrx flox,allele |
R2228 |
T4088 |
T4089 |
advmod |
functionally,normal |
R2229 |
T4089 |
T4080 |
acomp |
normal,was |
R2230 |
T4090 |
T4058 |
punct |
.,were |
R2231 |
T4092 |
T4093 |
aux |
To,generate |
R2232 |
T4093 |
T4094 |
advcl |
generate,crossed |
R2233 |
T4095 |
T4096 |
amod |
Atrxnull,mice |
R2234 |
T4096 |
T4093 |
dobj |
mice,generate |
R2235 |
T4097 |
T4093 |
prep |
by,generate |
R2236 |
T4098 |
T4099 |
npadvmod |
Cre,mediated |
R2237 |
T4099 |
T4101 |
amod |
mediated,recombination |
R2238 |
T4100 |
T4099 |
punct |
-,mediated |
R2239 |
T4101 |
T4097 |
pobj |
recombination,by |
R2240 |
T4102 |
T4094 |
punct |
", ",crossed |
R2241 |
T4103 |
T4104 |
det |
the,mice |
R2242 |
T4104 |
T4094 |
nsubjpass |
mice,crossed |
R2243 |
T4105 |
T4104 |
compound |
Atrx flox,mice |
R2244 |
T4106 |
T4094 |
auxpass |
were,crossed |
R2245 |
T4107 |
T4094 |
prep |
with,crossed |
R2246 |
T4108 |
T4107 |
pobj |
mice,with |
R2247 |
T4109 |
T4108 |
acl |
harboring,mice |
R2248 |
T4110 |
T4111 |
det |
a,transgene |
R2249 |
T4111 |
T4109 |
dobj |
transgene,harboring |
R2250 |
T4112 |
T4113 |
prep |
in,expressed |
R2251 |
T4113 |
T4111 |
relcl |
expressed,transgene |
R2252 |
T4114 |
T4112 |
pobj |
which,in |
R2253 |
T4115 |
T4116 |
det |
the,recombinase |
R2254 |
T4116 |
T4113 |
nsubjpass |
recombinase,expressed |
R2255 |
T4117 |
T4116 |
compound |
Cre,recombinase |
R2256 |
T4118 |
T4113 |
auxpass |
is,expressed |
R2257 |
T4119 |
T4113 |
prep |
under,expressed |
R2258 |
T4120 |
T4121 |
det |
the,control |
R2259 |
T4121 |
T4119 |
pobj |
control,under |
R2260 |
T4122 |
T4121 |
prep |
of,control |
R2261 |
T4123 |
T4124 |
det |
the,elements |
R2262 |
T4124 |
T4122 |
pobj |
elements,of |
R2263 |
T4125 |
T4124 |
amod |
regulatory,elements |
R2264 |
T4126 |
T4124 |
prep |
of,elements |
R2265 |
T4127 |
T4128 |
det |
the,gene |
R2266 |
T4128 |
T4126 |
pobj |
gene,of |
R2267 |
T4129 |
T4128 |
nmod |
mouse,gene |
R2268 |
T4130 |
T4128 |
nmod |
GATA,gene |
R2269 |
T4131 |
T4130 |
punct |
-,GATA |
R2270 |
T4132 |
T4130 |
nummod |
1,GATA |
R2271 |
T4133 |
T4128 |
punct |
(,gene |
R2272 |
T4134 |
T4135 |
compound |
GATA1,cre |
R2273 |
T4135 |
T4128 |
appos |
cre,gene |
R2274 |
T4136 |
T4135 |
punct |
-,cre |
R2275 |
T4137 |
T4094 |
punct |
),crossed |
R2276 |
T4138 |
T4139 |
punct |
[,14 |
R2277 |
T4139 |
T4094 |
parataxis |
14,crossed |
R2278 |
T4140 |
T4139 |
punct |
],14 |
R2279 |
T4141 |
T4094 |
punct |
.,crossed |
R2280 |
T4143 |
T4144 |
amod |
Widespread,expression |
R2281 |
T4144 |
T4145 |
nsubjpass |
expression,demonstrated |
R2282 |
T4146 |
T4144 |
prep |
of,expression |
R2283 |
T4147 |
T4148 |
det |
the,transgene |
R2284 |
T4148 |
T4146 |
pobj |
transgene,of |
R2285 |
T4149 |
T4150 |
compound |
GATA1,cre |
R2286 |
T4150 |
T4148 |
compound |
cre,transgene |
R2287 |
T4151 |
T4150 |
punct |
-,cre |
R2288 |
T4152 |
T4145 |
aux |
has,demonstrated |
R2289 |
T4153 |
T4145 |
auxpass |
been,demonstrated |
R2290 |
T4154 |
T4145 |
prep |
during,demonstrated |
R2291 |
T4155 |
T4156 |
amod |
early,embryogenesis |
R2292 |
T4156 |
T4154 |
pobj |
embryogenesis,during |
R2293 |
T4157 |
T4158 |
punct |
[,14 |
R2294 |
T4158 |
T4145 |
parataxis |
14,demonstrated |
R2295 |
T4159 |
T4158 |
punct |
],14 |
R2296 |
T4160 |
T4145 |
punct |
.,demonstrated |
R2297 |
T4162 |
T4163 |
nsubj |
We,defined |
R2298 |
T4164 |
T4165 |
advmod |
more,accurately |
R2299 |
T4165 |
T4163 |
advmod |
accurately,defined |
R2300 |
T4166 |
T4167 |
det |
the,onset |
R2301 |
T4167 |
T4163 |
dobj |
onset,defined |
R2302 |
T4168 |
T4167 |
prep |
of,onset |
R2303 |
T4169 |
T4170 |
compound |
GATA1,cre |
R2304 |
T4170 |
T4172 |
compound |
cre,expression |
R2305 |
T4171 |
T4170 |
punct |
-,cre |
R2306 |
T4172 |
T4168 |
pobj |
expression,of |
R2307 |
T4173 |
T4163 |
advcl |
using,defined |
R2308 |
T4174 |
T4175 |
det |
a,strain |
R2309 |
T4175 |
T4173 |
dobj |
strain,using |
R2310 |
T4176 |
T4175 |
compound |
ROSA26,strain |
R2311 |
T4177 |
T4175 |
compound |
reporter,strain |
R2312 |
T4178 |
T4175 |
punct |
", ",strain |
R2313 |
T4179 |
T4180 |
prep |
in,expressed |
R2314 |
T4180 |
T4175 |
relcl |
expressed,strain |
R2315 |
T4181 |
T4179 |
pobj |
which,in |
R2316 |
T4182 |
T4183 |
det |
a,gene |
R2317 |
T4183 |
T4180 |
nsubjpass |
gene,expressed |
R2318 |
T4184 |
T4185 |
nmod |
β,galactosidase |
R2319 |
T4185 |
T4187 |
nmod |
galactosidase,fusion |
R2320 |
T4186 |
T4185 |
punct |
-,galactosidase |
R2321 |
T4187 |
T4183 |
compound |
fusion,gene |
R2322 |
T4188 |
T4185 |
punct |
/,galactosidase |
R2323 |
T4189 |
T4185 |
appos |
neor,galactosidase |
R2324 |
T4190 |
T4183 |
compound |
reporter,gene |
R2325 |
T4191 |
T4180 |
auxpass |
is,expressed |
R2326 |
T4192 |
T4193 |
advmod |
only,after |
R2327 |
T4193 |
T4180 |
prep |
after,expressed |
R2328 |
T4194 |
T4195 |
npadvmod |
Cre,mediated |
R2329 |
T4195 |
T4197 |
amod |
mediated,excision |
R2330 |
T4196 |
T4195 |
punct |
-,mediated |
R2331 |
T4197 |
T4193 |
pobj |
excision,after |
R2332 |
T4198 |
T4197 |
prep |
of,excision |
R2333 |
T4199 |
T4200 |
npadvmod |
loxP,flanked |
R2334 |
T4200 |
T4202 |
amod |
flanked,transcription |
R2335 |
T4201 |
T4200 |
punct |
-,flanked |
R2336 |
T4202 |
T4198 |
pobj |
transcription,of |
R2337 |
T4203 |
T4197 |
cc |
and,excision |
R2338 |
T4204 |
T4205 |
compound |
translation,termination |
R2339 |
T4205 |
T4206 |
compound |
termination,signals |
R2340 |
T4206 |
T4197 |
conj |
signals,excision |
R2341 |
T4207 |
T4208 |
punct |
[,14 |
R2342 |
T4208 |
T4163 |
parataxis |
14,defined |
R2343 |
T4209 |
T4208 |
punct |
],14 |
R2344 |
T4210 |
T4163 |
punct |
.,defined |
R2345 |
T4212 |
T4213 |
nsubj |
We,found |
R2346 |
T4214 |
T4215 |
mark |
that,was |
R2347 |
T4215 |
T4213 |
ccomp |
was,found |
R2348 |
T4216 |
T4217 |
det |
the,transgene |
R2349 |
T4217 |
T4215 |
nsubj |
transgene,was |
R2350 |
T4218 |
T4219 |
compound |
GATA1,cre |
R2351 |
T4219 |
T4217 |
compound |
cre,transgene |
R2352 |
T4220 |
T4219 |
punct |
-,cre |
R2353 |
T4221 |
T4215 |
advmod |
already,was |
R2354 |
T4222 |
T4215 |
acomp |
active,was |
R2355 |
T4223 |
T4215 |
prep |
at,was |
R2356 |
T4224 |
T4225 |
det |
the,stage |
R2357 |
T4225 |
T4223 |
pobj |
stage,at |
R2358 |
T4226 |
T4227 |
nummod |
16,cell |
R2359 |
T4227 |
T4225 |
compound |
cell,stage |
R2360 |
T4228 |
T4227 |
punct |
-,cell |
R2361 |
T4229 |
T4225 |
compound |
morula,stage |
R2362 |
T4230 |
T4225 |
prep |
of,stage |
R2363 |
T4231 |
T4230 |
pobj |
development,of |
R2364 |
T4232 |
T4225 |
punct |
(,stage |
R2365 |
T4233 |
T4234 |
nummod |
0.5,days |
R2366 |
T4234 |
T4235 |
npadvmod |
days,postcoitus |
R2367 |
T4235 |
T4225 |
advmod |
postcoitus,stage |
R2368 |
T4236 |
T4237 |
punct |
[,dpc |
R2369 |
T4237 |
T4235 |
parataxis |
dpc,postcoitus |
R2370 |
T4238 |
T4237 |
punct |
],dpc |
R2371 |
T4239 |
T4213 |
punct |
),found |
R2372 |
T4240 |
T4241 |
punct |
(,4A |
R2373 |
T4241 |
T4213 |
parataxis |
4A,found |
R2374 |
T4242 |
T4241 |
compound |
Figure,4A |
R2375 |
T4243 |
T4241 |
punct |
),4A |
R2376 |
T4244 |
T4213 |
punct |
.,found |