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PMC:1435744 / 47111-48657 JSONTXT

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craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T27828 652-658 SO_EXT:0000440 denotes vector
T27829 0-10 GO_EXT:0045289 denotes Luciferase
T27830 21-36 GO_EXT:transcription denotes transcriptional
T27831 58-59 CHEBI_SO_EXT:base denotes b
T27832 60-68 SO_EXT:0000167 denotes promoter
T27833 79-83 PR_EXT:000014441 denotes mr-s
T27834 111-118 SO_EXT:0000155 denotes plasmid
T27835 150-151 CHEBI_SO_EXT:base denotes b
T27836 152-160 SO_EXT:sequence_upstreamness denotes upstream
T27837 161-168 SO_EXT:0001026 denotes genomic
T27838 169-180 _FRAGMENT denotes fragment of
T27839 186-190 SO_EXT:0000997 denotes gene
T27840 181-185 PR_EXT:000014441 denotes mr-s
T27841 203-213 GO_EXT:0045289 denotes luciferase
T27842 223-230 SO_EXT:0000155 denotes plasmid
T27843 246-256 GO:0010467 denotes expression
T27844 257-264 SO_EXT:0000440 denotes vectors
T27845 308-311 PR_EXT:000005904 denotes Crx
T27846 313-317 PR_EXT:000012086 denotes Otx2
T27847 319-322 PR_EXT:000011432 denotes Nrl
T27848 323-328 SO_EXT:0000704 denotes genes
T27849 341-351 GO:0010467 denotes expression
T27850 352-358 SO_EXT:0000440 denotes vector
T27851 422-428 SO_EXT:0000440 denotes vector
T27852 434-437 PR_EXT:000005904 denotes Crx
T27853 438-445 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T27854 438-450 SO_EXT:0000409 denotes binding site
T27855 464-466 SO_EXT:0000028 denotes bp
T27856 480-487 SO_EXT:sequence_alteration_process denotes mutated
T27857 491-500 SO_EXT:sequence_substitution_process denotes replacing
T27858 538-544 SO_EXT:0000440 denotes vector
T27859 550-553 PR_EXT:000005904 denotes Crx
T27860 554-561 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T27861 554-566 SO_EXT:0000409 denotes binding site
T27862 579-581 SO_EXT:0000028 denotes bp
T27863 595-602 SO_EXT:sequence_alteration_process denotes mutated
T27864 606-615 SO_EXT:sequence_substitution_process denotes replacing
T27865 664-667 PR_EXT:000005904 denotes Crx
T27866 668-675 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T27867 668-680 SO_EXT:0000409 denotes binding site
T27868 692-694 SO_EXT:0000028 denotes bp
T27869 708-715 SO_EXT:sequence_alteration_process denotes mutated
T27870 719-728 SO_EXT:sequence_substitution_process denotes replacing
T27871 758-761 SO_EXT:sequence_altered_entity_or_alteration_process denotes mut
T27872 767-773 SO_EXT:0000440 denotes vector
T27873 788-791 PR_EXT:000005904 denotes Crx
T27874 792-799 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T27875 792-805 SO_EXT:0000409 denotes binding sites
T27876 811-818 SO_EXT:sequence_alteration_process denotes mutated
T27877 849-856 SO_EXT:0000155 denotes plasmid
T27878 878-884 SO_EXT:sequence_copy_entity denotes copies
T27879 892-896 PR_EXT:P04386 denotes GAL4
T27880 897-900 CHEBI_SO_EXT:DNA denotes DNA
T27881 913-921 SO_EXT:biological_sequence denotes sequence
T27882 945-953 SO_EXT:sequence_upstreamness denotes upstream
T27883 957-961 NCBITaxon:10633 denotes SV40
T27884 970-978 SO_EXT:0000167 denotes promoter
T27885 1047-1062 GO_EXT:transcription denotes transcriptional
T27886 1075-1079 PR_EXT:000014441 denotes mr-s
T27887 1098-1106 CHEBI_EXT:35224 denotes effector
T27888 1107-1115 SO_EXT:0000155 denotes plasmids
T27889 1125-1133 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T27890 1200-1206 SO_EXT:0000440 denotes vector
T27891 1261-1269 SO_EXT:biological_sequence denotes sequence
T27892 1277-1284 SO_EXT:sequence_coding_function denotes encodes
T27893 1285-1289 PR_EXT:P04386 denotes GAL4
T27894 1290-1293 CHEBI_SO_EXT:DNA denotes DNA
T27895 1294-1301 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T27896 1302-1308 SO_EXT:0000417 denotes domain
T27897 1314-1322 SO_EXT:sequence_insertion_process denotes inserted
T27898 1339-1350 GO:0009294 denotes transfected
T27899 1370-1377 SO_EXT:0000155 denotes plasmid
T27900 1378-1381 CHEBI_SO_EXT:DNA denotes DNA
T27901 1398-1408 GO:0010467 denotes expression
T27902 1409-1415 SO_EXT:0000440 denotes vector
T27903 1416-1419 CHEBI_SO_EXT:DNA denotes DNA
T27904 1447-1452 CL_GO_EXT:cell denotes cells
T27905 1467-1479 GO:0009294 denotes transfection
T27906 1480-1487 CHEBI_EXT:33893 denotes reagent
T27907 1501-1511 GO_EXT:0045289 denotes luciferase
T27908 1533-1545 GO:0009294 denotes transfection
R7869 T27839 T27838 _lexicallyChainedTo gene,fragment of

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T27780 60-68 SO:0000167 denotes promoter
T27781 79-83 PR:000014441 denotes mr-s
T27782 111-118 SO:0000155 denotes plasmid
T27783 161-168 SO:0001026 denotes genomic
T27784 169-180 _FRAGMENT denotes fragment of
T27785 186-190 SO:0000997 denotes gene
T27786 181-185 PR:000014441 denotes mr-s
T27787 223-230 SO:0000155 denotes plasmid
T27788 246-256 GO:0010467 denotes expression
T27789 257-264 SO:0000440 denotes vectors
T27790 308-311 PR:000005904 denotes Crx
T27791 313-317 PR:000012086 denotes Otx2
T27792 319-322 PR:000011432 denotes Nrl
T27793 323-328 SO:0000704 denotes genes
T27794 341-351 GO:0010467 denotes expression
T27795 352-358 SO:0000440 denotes vector
T27796 422-428 SO:0000440 denotes vector
T27797 434-437 PR:000005904 denotes Crx
T27798 438-450 SO:0000409 denotes binding site
T27799 464-466 SO:0000028 denotes bp
T27800 538-544 SO:0000440 denotes vector
T27801 550-553 PR:000005904 denotes Crx
T27802 554-566 SO:0000409 denotes binding site
T27803 579-581 SO:0000028 denotes bp
T27804 652-658 SO:0000440 denotes vector
T27805 664-667 PR:000005904 denotes Crx
T27806 668-680 SO:0000409 denotes binding site
T27807 692-694 SO:0000028 denotes bp
T27808 767-773 SO:0000440 denotes vector
T27809 788-791 PR:000005904 denotes Crx
T27810 792-805 SO:0000409 denotes binding sites
T27811 849-856 SO:0000155 denotes plasmid
T27812 892-896 PR:P04386 denotes GAL4
T27813 957-961 NCBITaxon:10633 denotes SV40
T27814 970-978 SO:0000167 denotes promoter
T27815 1075-1079 PR:000014441 denotes mr-s
T27816 1098-1106 CHEBI:35224 denotes effector
T27817 1107-1115 SO:0000155 denotes plasmids
T27818 1200-1206 SO:0000440 denotes vector
T27819 1285-1289 PR:P04386 denotes GAL4
T27820 1302-1308 SO:0000417 denotes domain
T27821 1339-1350 GO:0009294 denotes transfected
T27822 1370-1377 SO:0000155 denotes plasmid
T27823 1398-1408 GO:0010467 denotes expression
T27824 1409-1415 SO:0000440 denotes vector
T27825 1467-1479 GO:0009294 denotes transfection
T27826 1480-1487 CHEBI:33893 denotes reagent
T27827 1533-1545 GO:0009294 denotes transfection
R7868 T27785 T27784 _lexicallyChainedTo gene,fragment of

craft-sa-dev

Id Subject Object Predicate Lexical cue
T27909 0-10 NN denotes Luciferase
T27910 11-16 NN denotes assay
T27911 16-241 sentence denotes For transcriptional analysis of the 1.2-kb promoter region of mr-s, we constructed a reporter plasmid (pro1.2k) by subcloning a 1.2-kb upstream genomic fragment of mr-s gene into a pGL3 luciferase reporter plasmid (Promega).
T27912 17-20 IN denotes For
T27913 88-99 VBD denotes constructed
T27914 21-36 JJ denotes transcriptional
T27915 37-45 NN denotes analysis
T27916 46-48 IN denotes of
T27917 49-52 DT denotes the
T27918 69-75 NN denotes region
T27919 53-56 CD denotes 1.2
T27920 57-59 NN denotes kb
T27921 56-57 HYPH denotes -
T27922 60-68 NN denotes promoter
T27923 76-78 IN denotes of
T27924 79-81 NN denotes mr
T27925 82-83 NN denotes s
T27926 81-82 HYPH denotes -
T27927 83-85 , denotes ,
T27928 85-87 PRP denotes we
T27929 100-101 DT denotes a
T27930 111-118 NN denotes plasmid
T27931 102-110 NN denotes reporter
T27932 119-120 -LRB- denotes (
T27933 120-127 NN denotes pro1.2k
T27934 127-128 -RRB- denotes )
T27935 129-131 IN denotes by
T27936 132-142 VBG denotes subcloning
T27937 143-144 DT denotes a
T27938 169-177 NN denotes fragment
T27939 145-148 CD denotes 1.2
T27940 149-151 NN denotes kb
T27941 148-149 HYPH denotes -
T27942 152-160 JJ denotes upstream
T27943 161-168 JJ denotes genomic
T27944 178-180 IN denotes of
T27945 181-183 NN denotes mr
T27946 184-185 NN denotes s
T27947 183-184 HYPH denotes -
T27948 186-190 NN denotes gene
T27949 191-195 IN denotes into
T27950 196-197 DT denotes a
T27951 223-230 NN denotes plasmid
T27952 198-202 NN denotes pGL3
T27953 214-222 NN denotes reporter
T27954 203-213 NN denotes luciferase
T27955 231-232 -LRB- denotes (
T27956 232-239 NNP denotes Promega
T27957 239-240 -RRB- denotes )
T27958 240-241 . denotes .
T27959 241-403 sentence denotes The expression vectors were constructed by subcloning full-length Crx, Otx2, Nrl genes into a pMIK expression vector (a gift from Dr. K. Maruyama), respectively.
T27960 242-245 DT denotes The
T27961 257-264 NNS denotes vectors
T27962 246-256 NN denotes expression
T27963 270-281 VBN denotes constructed
T27964 265-269 VBD denotes were
T27965 282-284 IN denotes by
T27966 285-295 VBG denotes subcloning
T27967 296-300 JJ denotes full
T27968 301-307 NN denotes length
T27969 300-301 HYPH denotes -
T27970 323-328 NNS denotes genes
T27971 308-311 NN denotes Crx
T27972 319-322 NN denotes Nrl
T27973 311-313 , denotes ,
T27974 313-317 NN denotes Otx2
T27975 317-319 , denotes ,
T27976 329-333 IN denotes into
T27977 334-335 DT denotes a
T27978 352-358 NN denotes vector
T27979 336-340 NN denotes pMIK
T27980 341-351 NN denotes expression
T27981 359-360 -LRB- denotes (
T27982 362-366 NN denotes gift
T27983 360-361 DT denotes a
T27984 367-371 IN denotes from
T27985 372-375 NNP denotes Dr.
T27986 379-387 NNP denotes Maruyama
T27987 376-378 NNP denotes K.
T27988 387-388 -RRB- denotes )
T27989 388-390 , denotes ,
T27990 390-402 RB denotes respectively
T27991 402-403 . denotes .
T27992 403-520 sentence denotes For the "mut1259" vector, the Crx binding site at the -1259 bp position was mutated by replacing GGATTA with AGATCT.
T27993 404-407 IN denotes For
T27994 480-487 VBN denotes mutated
T27995 408-411 DT denotes the
T27996 422-428 NN denotes vector
T27997 412-413 `` denotes "
T27998 413-420 NN denotes mut1259
T27999 420-421 '' denotes "
T28000 428-430 , denotes ,
T28001 430-433 DT denotes the
T28002 446-450 NN denotes site
T28003 434-437 NN denotes Crx
T28004 438-445 NN denotes binding
T28005 451-453 IN denotes at
T28006 454-457 DT denotes the
T28007 467-475 NN denotes position
T28008 458-459 SYM denotes -
T28009 459-463 CD denotes 1259
T28010 464-466 NN denotes bp
T28011 476-479 VBD denotes was
T28012 488-490 IN denotes by
T28013 491-500 VBG denotes replacing
T28014 501-507 NN denotes GGATTA
T28015 508-512 IN denotes with
T28016 513-519 NN denotes AGATCT
T28017 519-520 . denotes .
T28018 520-635 sentence denotes For the "mut198" vector, the Crx binding site at the -198 bp position was mutated by replacing TAATCC with GAATTC.
T28019 521-524 IN denotes For
T28020 595-602 VBN denotes mutated
T28021 525-528 DT denotes the
T28022 538-544 NN denotes vector
T28023 529-530 `` denotes "
T28024 530-536 NN denotes mut198
T28025 536-537 '' denotes "
T28026 544-546 , denotes ,
T28027 546-549 DT denotes the
T28028 562-566 NN denotes site
T28029 550-553 NN denotes Crx
T28030 554-561 NN denotes binding
T28031 567-569 IN denotes at
T28032 570-573 DT denotes the
T28033 582-590 NN denotes position
T28034 574-575 SYM denotes -
T28035 575-578 CD denotes 198
T28036 579-581 NN denotes bp
T28037 591-594 VBD denotes was
T28038 603-605 IN denotes by
T28039 606-615 VBG denotes replacing
T28040 616-622 NN denotes TAATCC
T28041 623-627 IN denotes with
T28042 628-634 NN denotes GAATTC
T28043 634-635 . denotes .
T28044 635-748 sentence denotes For the "mut72" vector, the Crx binding site at the -72 bp position was mutated by replacing GGATTA with GAATTC.
T28045 636-639 IN denotes For
T28046 708-715 VBN denotes mutated
T28047 640-643 DT denotes the
T28048 652-658 NN denotes vector
T28049 644-645 `` denotes "
T28050 645-650 NN denotes mut72
T28051 650-651 '' denotes "
T28052 658-660 , denotes ,
T28053 660-663 DT denotes the
T28054 676-680 NN denotes site
T28055 664-667 NN denotes Crx
T28056 668-675 NN denotes binding
T28057 681-683 IN denotes at
T28058 684-687 DT denotes the
T28059 695-703 NN denotes position
T28060 688-689 SYM denotes -
T28061 689-691 CD denotes 72
T28062 692-694 NN denotes bp
T28063 704-707 VBD denotes was
T28064 716-718 IN denotes by
T28065 719-728 VBG denotes replacing
T28066 729-735 NN denotes GGATTA
T28067 736-740 IN denotes with
T28068 741-747 NN denotes GAATTC
T28069 747-748 . denotes .
T28070 748-819 sentence denotes For the "mut all" vector, all of three Crx binding sites were mutated.
T28071 749-752 IN denotes For
T28072 811-818 VBN denotes mutated
T28073 753-756 DT denotes the
T28074 767-773 NN denotes vector
T28075 757-758 `` denotes "
T28076 758-761 NN denotes mut
T28077 762-765 NN denotes all
T28078 765-766 '' denotes "
T28079 773-775 , denotes ,
T28080 775-778 DT denotes all
T28081 779-781 IN denotes of
T28082 782-787 CD denotes three
T28083 800-805 NNS denotes sites
T28084 788-791 NN denotes Crx
T28085 792-799 NN denotes binding
T28086 806-810 VBD denotes were
T28087 818-819 . denotes .
T28088 819-1085 sentence denotes 5xGAL4-pGL3 Control reporter plasmid, which contains five copies of the GAL4 DNA recognition sequence positioned immediately upstream of SV40 minimal promoter, was kindly gifted from Dr. T. Noguchi and used for analysis of the transcriptional activity of mr-s [50].
T28089 820-826 NN denotes 5xGAL4
T28090 827-831 NN denotes pGL3
T28091 826-827 HYPH denotes -
T28092 849-856 NN denotes plasmid
T28093 832-839 NN denotes Control
T28094 840-848 NN denotes reporter
T28095 991-997 VBN denotes gifted
T28096 856-858 , denotes ,
T28097 858-863 WDT denotes which
T28098 864-872 VBZ denotes contains
T28099 873-877 CD denotes five
T28100 878-884 NNS denotes copies
T28101 885-887 IN denotes of
T28102 888-891 DT denotes the
T28103 913-921 NN denotes sequence
T28104 892-896 NN denotes GAL4
T28105 897-900 NN denotes DNA
T28106 901-912 NN denotes recognition
T28107 922-932 VBN denotes positioned
T28108 933-944 RB denotes immediately
T28109 945-953 IN denotes upstream
T28110 954-956 IN denotes of
T28111 957-961 NN denotes SV40
T28112 970-978 NN denotes promoter
T28113 962-969 JJ denotes minimal
T28114 978-980 , denotes ,
T28115 980-983 VBD denotes was
T28116 984-990 RB denotes kindly
T28117 998-1002 IN denotes from
T28118 1003-1006 NNP denotes Dr.
T28119 1010-1017 NNP denotes Noguchi
T28120 1007-1009 NNP denotes T.
T28121 1018-1021 CC denotes and
T28122 1022-1026 VBN denotes used
T28123 1027-1030 IN denotes for
T28124 1031-1039 NN denotes analysis
T28125 1040-1042 IN denotes of
T28126 1043-1046 DT denotes the
T28127 1063-1071 NN denotes activity
T28128 1047-1062 JJ denotes transcriptional
T28129 1072-1074 IN denotes of
T28130 1075-1077 NN denotes mr
T28131 1078-1079 NN denotes s
T28132 1077-1078 HYPH denotes -
T28133 1080-1081 -LRB- denotes [
T28134 1081-1083 CD denotes 50
T28135 1083-1084 -RRB- denotes ]
T28136 1084-1085 . denotes .
T28137 1085-1221 sentence denotes To generate effector plasmids, various deletion fragments were produced by PCR reaction and subcloned into pGBKT7 vector, respectively.
T28138 1086-1088 TO denotes To
T28139 1089-1097 VB denotes generate
T28140 1149-1157 VBN denotes produced
T28141 1098-1106 NN denotes effector
T28142 1107-1115 NNS denotes plasmids
T28143 1115-1117 , denotes ,
T28144 1117-1124 JJ denotes various
T28145 1134-1143 NNS denotes fragments
T28146 1125-1133 NN denotes deletion
T28147 1144-1148 VBD denotes were
T28148 1158-1160 IN denotes by
T28149 1161-1164 NN denotes PCR
T28150 1165-1173 NN denotes reaction
T28151 1174-1177 CC denotes and
T28152 1178-1187 VBN denotes subcloned
T28153 1188-1192 IN denotes into
T28154 1193-1199 NN denotes pGBKT7
T28155 1200-1206 NN denotes vector
T28156 1206-1208 , denotes ,
T28157 1208-1220 RB denotes respectively
T28158 1220-1221 . denotes .
T28159 1221-1335 sentence denotes These fragments were digested with the sequence, which encodes GAL4 DNA binding domain, and inserted into pcDNA3.
T28160 1222-1227 DT denotes These
T28161 1228-1237 NNS denotes fragments
T28162 1243-1251 VBN denotes digested
T28163 1238-1242 VBD denotes were
T28164 1252-1256 IN denotes with
T28165 1257-1260 DT denotes the
T28166 1261-1269 NN denotes sequence
T28167 1269-1271 , denotes ,
T28168 1271-1276 WDT denotes which
T28169 1277-1284 VBZ denotes encodes
T28170 1285-1289 NN denotes GAL4
T28171 1302-1308 NN denotes domain
T28172 1290-1293 NN denotes DNA
T28173 1294-1301 NN denotes binding
T28174 1308-1310 , denotes ,
T28175 1310-1313 CC denotes and
T28176 1314-1322 VBN denotes inserted
T28177 1323-1327 IN denotes into
T28178 1328-1334 NN denotes pcDNA3
T28179 1334-1335 . denotes .
T28180 1335-1488 sentence denotes We transfected 0.1 μg of reporter plasmid DNA and 2 μg of the expression vector DNA per 6 cm dish into HEK293T cells using Fugene6 transfection reagent.
T28181 1336-1338 PRP denotes We
T28182 1339-1350 VBD denotes transfected
T28183 1351-1354 CD denotes 0.1
T28184 1355-1357 NN denotes μg
T28185 1358-1360 IN denotes of
T28186 1361-1369 NN denotes reporter
T28187 1378-1381 NN denotes DNA
T28188 1370-1377 NN denotes plasmid
T28189 1382-1385 CC denotes and
T28190 1386-1387 CD denotes 2
T28191 1388-1390 NN denotes μg
T28192 1391-1393 IN denotes of
T28193 1394-1397 DT denotes the
T28194 1416-1419 NN denotes DNA
T28195 1398-1408 NN denotes expression
T28196 1409-1415 NN denotes vector
T28197 1420-1423 IN denotes per
T28198 1424-1425 CD denotes 6
T28199 1426-1428 NN denotes cm
T28200 1429-1433 NN denotes dish
T28201 1434-1438 IN denotes into
T28202 1439-1446 NN denotes HEK293T
T28203 1447-1452 NNS denotes cells
T28204 1453-1458 VBG denotes using
T28205 1459-1466 NN denotes Fugene6
T28206 1480-1487 NN denotes reagent
T28207 1467-1479 NN denotes transfection
T28208 1487-1488 . denotes .
T28209 1488-1546 sentence denotes We analyzed luciferase activity 48 hr after transfection.
T28210 1489-1491 PRP denotes We
T28211 1492-1500 VBD denotes analyzed
T28212 1501-1511 NN denotes luciferase
T28213 1512-1520 NN denotes activity
T28214 1521-1523 CD denotes 48
T28215 1524-1526 NN denotes hr
T28216 1527-1532 IN denotes after
T28217 1533-1545 NN denotes transfection
T28218 1545-1546 . denotes .
R7870 T27909 T27910 compound Luciferase,assay
R7871 T27912 T27913 prep For,constructed
R7872 T27914 T27915 amod transcriptional,analysis
R7873 T27915 T27912 pobj analysis,For
R7874 T27916 T27915 prep of,analysis
R7875 T27917 T27918 det the,region
R7876 T27918 T27916 pobj region,of
R7877 T27919 T27920 nummod 1.2,kb
R7878 T27920 T27918 compound kb,region
R7879 T27921 T27920 punct -,kb
R7880 T27922 T27918 compound promoter,region
R7881 T27923 T27918 prep of,region
R7882 T27924 T27925 compound mr,s
R7883 T27925 T27923 pobj s,of
R7884 T27926 T27925 punct -,s
R7885 T27927 T27913 punct ", ",constructed
R7886 T27928 T27913 nsubj we,constructed
R7887 T27929 T27930 det a,plasmid
R7888 T27930 T27913 dobj plasmid,constructed
R7889 T27931 T27930 compound reporter,plasmid
R7890 T27932 T27933 punct (,pro1.2k
R7891 T27933 T27930 parataxis pro1.2k,plasmid
R7892 T27934 T27933 punct ),pro1.2k
R7893 T27935 T27913 prep by,constructed
R7894 T27936 T27935 pcomp subcloning,by
R7895 T27937 T27938 det a,fragment
R7896 T27938 T27936 dobj fragment,subcloning
R7897 T27939 T27940 nummod 1.2,kb
R7898 T27940 T27938 nmod kb,fragment
R7899 T27941 T27940 punct -,kb
R7900 T27942 T27938 amod upstream,fragment
R7901 T27943 T27938 amod genomic,fragment
R7902 T27944 T27938 prep of,fragment
R7903 T27945 T27946 compound mr,s
R7904 T27946 T27948 compound s,gene
R7905 T27947 T27946 punct -,s
R7906 T27948 T27944 pobj gene,of
R7907 T27949 T27936 prep into,subcloning
R7908 T27950 T27951 det a,plasmid
R7909 T27951 T27949 pobj plasmid,into
R7910 T27952 T27953 compound pGL3,reporter
R7911 T27953 T27951 compound reporter,plasmid
R7912 T27954 T27953 compound luciferase,reporter
R7913 T27955 T27956 punct (,Promega
R7914 T27956 T27951 parataxis Promega,plasmid
R7915 T27957 T27956 punct ),Promega
R7916 T27958 T27913 punct .,constructed
R7917 T27960 T27961 det The,vectors
R7918 T27961 T27963 nsubjpass vectors,constructed
R7919 T27962 T27961 compound expression,vectors
R7920 T27964 T27963 auxpass were,constructed
R7921 T27965 T27963 prep by,constructed
R7922 T27966 T27965 pcomp subcloning,by
R7923 T27967 T27968 amod full,length
R7924 T27968 T27970 nmod length,genes
R7925 T27969 T27968 punct -,length
R7926 T27970 T27966 dobj genes,subcloning
R7927 T27971 T27972 nmod Crx,Nrl
R7928 T27972 T27970 compound Nrl,genes
R7929 T27973 T27972 punct ", ",Nrl
R7930 T27974 T27972 nmod Otx2,Nrl
R7931 T27975 T27972 punct ", ",Nrl
R7932 T27976 T27966 prep into,subcloning
R7933 T27977 T27978 det a,vector
R7934 T27978 T27976 pobj vector,into
R7935 T27979 T27978 compound pMIK,vector
R7936 T27980 T27978 compound expression,vector
R7937 T27981 T27982 punct (,gift
R7938 T27982 T27978 parataxis gift,vector
R7939 T27983 T27982 det a,gift
R7940 T27984 T27982 prep from,gift
R7941 T27985 T27986 compound Dr.,Maruyama
R7942 T27986 T27984 pobj Maruyama,from
R7943 T27987 T27986 compound K.,Maruyama
R7944 T27988 T27982 punct ),gift
R7945 T27989 T27966 punct ", ",subcloning
R7946 T27990 T27966 advmod respectively,subcloning
R7947 T27991 T27963 punct .,constructed
R7948 T27993 T27994 prep For,mutated
R7949 T27995 T27996 det the,vector
R7950 T27996 T27993 pobj vector,For
R7951 T27997 T27996 punct """",vector
R7952 T27998 T27996 nmod mut1259,vector
R7953 T27999 T27996 punct """",vector
R7954 T28000 T27994 punct ", ",mutated
R7955 T28001 T28002 det the,site
R7956 T28002 T27994 nsubjpass site,mutated
R7957 T28003 T28004 compound Crx,binding
R7958 T28004 T28002 compound binding,site
R7959 T28005 T28002 prep at,site
R7960 T28006 T28007 det the,position
R7961 T28007 T28005 pobj position,at
R7962 T28008 T28009 punct -,1259
R7963 T28009 T28010 nummod 1259,bp
R7964 T28010 T28007 compound bp,position
R7965 T28011 T27994 auxpass was,mutated
R7966 T28012 T27994 prep by,mutated
R7967 T28013 T28012 pcomp replacing,by
R7968 T28014 T28013 dobj GGATTA,replacing
R7969 T28015 T28013 prep with,replacing
R7970 T28016 T28015 pobj AGATCT,with
R7971 T28017 T27994 punct .,mutated
R7972 T28019 T28020 prep For,mutated
R7973 T28021 T28022 det the,vector
R7974 T28022 T28019 pobj vector,For
R7975 T28023 T28022 punct """",vector
R7976 T28024 T28022 nmod mut198,vector
R7977 T28025 T28022 punct """",vector
R7978 T28026 T28020 punct ", ",mutated
R7979 T28027 T28028 det the,site
R7980 T28028 T28020 nsubjpass site,mutated
R7981 T28029 T28030 compound Crx,binding
R7982 T28030 T28028 compound binding,site
R7983 T28031 T28028 prep at,site
R7984 T28032 T28033 det the,position
R7985 T28033 T28031 pobj position,at
R7986 T28034 T28035 punct -,198
R7987 T28035 T28036 nummod 198,bp
R7988 T28036 T28033 compound bp,position
R7989 T28037 T28020 auxpass was,mutated
R7990 T28038 T28020 prep by,mutated
R7991 T28039 T28038 pcomp replacing,by
R7992 T28040 T28039 dobj TAATCC,replacing
R7993 T28041 T28039 prep with,replacing
R7994 T28042 T28041 pobj GAATTC,with
R7995 T28043 T28020 punct .,mutated
R7996 T28045 T28046 prep For,mutated
R7997 T28047 T28048 det the,vector
R7998 T28048 T28045 pobj vector,For
R7999 T28049 T28048 punct """",vector
R8000 T28050 T28048 nmod mut72,vector
R8001 T28051 T28048 punct """",vector
R8002 T28052 T28046 punct ", ",mutated
R8003 T28053 T28054 det the,site
R8004 T28054 T28046 nsubjpass site,mutated
R8005 T28055 T28056 compound Crx,binding
R8006 T28056 T28054 compound binding,site
R8007 T28057 T28054 prep at,site
R8008 T28058 T28059 det the,position
R8009 T28059 T28057 pobj position,at
R8010 T28060 T28061 punct -,72
R8011 T28061 T28062 nummod 72,bp
R8012 T28062 T28059 compound bp,position
R8013 T28063 T28046 auxpass was,mutated
R8014 T28064 T28046 prep by,mutated
R8015 T28065 T28064 pcomp replacing,by
R8016 T28066 T28065 dobj GGATTA,replacing
R8017 T28067 T28065 prep with,replacing
R8018 T28068 T28067 pobj GAATTC,with
R8019 T28069 T28046 punct .,mutated
R8020 T28071 T28072 prep For,mutated
R8021 T28073 T28074 det the,vector
R8022 T28074 T28071 pobj vector,For
R8023 T28075 T28074 punct """",vector
R8024 T28076 T28077 nmod mut,all
R8025 T28077 T28074 nmod all,vector
R8026 T28078 T28074 punct """",vector
R8027 T28079 T28072 punct ", ",mutated
R8028 T28080 T28072 nsubjpass all,mutated
R8029 T28081 T28080 prep of,all
R8030 T28082 T28083 nummod three,sites
R8031 T28083 T28081 pobj sites,of
R8032 T28084 T28085 compound Crx,binding
R8033 T28085 T28083 compound binding,sites
R8034 T28086 T28072 auxpass were,mutated
R8035 T28087 T28072 punct .,mutated
R8036 T28089 T28090 compound 5xGAL4,pGL3
R8037 T28090 T28092 compound pGL3,plasmid
R8038 T28091 T28090 punct -,pGL3
R8039 T28092 T28095 nsubjpass plasmid,gifted
R8040 T28093 T28092 compound Control,plasmid
R8041 T28094 T28092 compound reporter,plasmid
R8042 T28096 T28092 punct ", ",plasmid
R8043 T28097 T28098 dep which,contains
R8044 T28098 T28092 relcl contains,plasmid
R8045 T28099 T28100 nummod five,copies
R8046 T28100 T28098 dobj copies,contains
R8047 T28101 T28100 prep of,copies
R8048 T28102 T28103 det the,sequence
R8049 T28103 T28101 pobj sequence,of
R8050 T28104 T28103 compound GAL4,sequence
R8051 T28105 T28106 compound DNA,recognition
R8052 T28106 T28103 compound recognition,sequence
R8053 T28107 T28100 acl positioned,copies
R8054 T28108 T28107 advmod immediately,positioned
R8055 T28109 T28108 advmod upstream,immediately
R8056 T28110 T28108 prep of,immediately
R8057 T28111 T28112 nmod SV40,promoter
R8058 T28112 T28110 pobj promoter,of
R8059 T28113 T28112 amod minimal,promoter
R8060 T28114 T28095 punct ", ",gifted
R8061 T28115 T28095 auxpass was,gifted
R8062 T28116 T28095 advmod kindly,gifted
R8063 T28117 T28095 agent from,gifted
R8064 T28118 T28119 compound Dr.,Noguchi
R8065 T28119 T28117 pobj Noguchi,from
R8066 T28120 T28119 compound T.,Noguchi
R8067 T28121 T28095 cc and,gifted
R8068 T28122 T28095 conj used,gifted
R8069 T28123 T28122 prep for,used
R8070 T28124 T28123 pobj analysis,for
R8071 T28125 T28124 prep of,analysis
R8072 T28126 T28127 det the,activity
R8073 T28127 T28125 pobj activity,of
R8074 T28128 T28127 amod transcriptional,activity
R8075 T28129 T28127 prep of,activity
R8076 T28130 T28131 compound mr,s
R8077 T28131 T28129 pobj s,of
R8078 T28132 T28131 punct -,s
R8079 T28133 T28134 punct [,50
R8080 T28134 T28122 parataxis 50,used
R8081 T28135 T28134 punct ],50
R8082 T28136 T28095 punct .,gifted
R8083 T28138 T28139 aux To,generate
R8084 T28139 T28140 advcl generate,produced
R8085 T28141 T28142 compound effector,plasmids
R8086 T28142 T28139 dobj plasmids,generate
R8087 T28143 T28140 punct ", ",produced
R8088 T28144 T28145 amod various,fragments
R8089 T28145 T28140 nsubj fragments,produced
R8090 T28146 T28145 compound deletion,fragments
R8091 T28147 T28140 aux were,produced
R8092 T28148 T28140 prep by,produced
R8093 T28149 T28150 compound PCR,reaction
R8094 T28150 T28148 pobj reaction,by
R8095 T28151 T28140 cc and,produced
R8096 T28152 T28140 conj subcloned,produced
R8097 T28153 T28152 prep into,subcloned
R8098 T28154 T28155 compound pGBKT7,vector
R8099 T28155 T28153 pobj vector,into
R8100 T28156 T28140 punct ", ",produced
R8101 T28157 T28140 advmod respectively,produced
R8102 T28158 T28140 punct .,produced
R8103 T28160 T28161 det These,fragments
R8104 T28161 T28162 nsubjpass fragments,digested
R8105 T28163 T28162 auxpass were,digested
R8106 T28164 T28162 prep with,digested
R8107 T28165 T28166 det the,sequence
R8108 T28166 T28164 pobj sequence,with
R8109 T28167 T28166 punct ", ",sequence
R8110 T28168 T28169 dep which,encodes
R8111 T28169 T28166 relcl encodes,sequence
R8112 T28170 T28171 compound GAL4,domain
R8113 T28171 T28169 dobj domain,encodes
R8114 T28172 T28173 compound DNA,binding
R8115 T28173 T28171 compound binding,domain
R8116 T28174 T28162 punct ", ",digested
R8117 T28175 T28162 cc and,digested
R8118 T28176 T28162 conj inserted,digested
R8119 T28177 T28176 prep into,inserted
R8120 T28178 T28177 pobj pcDNA3,into
R8121 T28179 T28162 punct .,digested
R8122 T28181 T28182 nsubj We,transfected
R8123 T28183 T28184 nummod 0.1,μg
R8124 T28184 T28182 dobj μg,transfected
R8125 T28185 T28184 prep of,μg
R8126 T28186 T28187 compound reporter,DNA
R8127 T28187 T28185 pobj DNA,of
R8128 T28188 T28187 compound plasmid,DNA
R8129 T28189 T28184 cc and,μg
R8130 T28190 T28191 nummod 2,μg
R8131 T28191 T28184 conj μg,μg
R8132 T28192 T28191 prep of,μg
R8133 T28193 T28194 det the,DNA
R8134 T28194 T28192 pobj DNA,of
R8135 T28195 T28196 compound expression,vector
R8136 T28196 T28194 compound vector,DNA
R8137 T28197 T28184 prep per,μg
R8138 T28198 T28199 nummod 6,cm
R8139 T28199 T28200 compound cm,dish
R8140 T28200 T28197 pobj dish,per
R8141 T28201 T28182 prep into,transfected
R8142 T28202 T28203 compound HEK293T,cells
R8143 T28203 T28201 pobj cells,into
R8144 T28204 T28182 advcl using,transfected
R8145 T28205 T28206 compound Fugene6,reagent
R8146 T28206 T28204 dobj reagent,using
R8147 T28207 T28206 compound transfection,reagent
R8148 T28208 T28182 punct .,transfected
R8149 T28210 T28211 nsubj We,analyzed
R8150 T28212 T28213 compound luciferase,activity
R8151 T28213 T28211 dobj activity,analyzed
R8152 T28214 T28215 nummod 48,hr
R8153 T28215 T28216 npadvmod hr,after
R8154 T28216 T28211 prep after,analyzed
R8155 T28217 T28216 pobj transfection,after
R8156 T28218 T28211 punct .,analyzed