
PMC:1435744 / 46219-46495
Annnotations
craft-ca-core-ex-dev
Below, discontinuous spans are shown in the chain model. You can change it to the bag model.
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T27000 | 4-15 | GO:0009294 | denotes | transfected |
T27001 | 20-27 | SO_EXT:0000155 | denotes | plasmid |
T27002 | 28-36 | SO_EXT:sequence_coding_function | denotes | encoding |
T27003 | 40-46 | CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process | denotes | tagged |
T27004 | 59-63 | PR_EXT:000014441 | denotes | mr-s |
T27005 | 77-82 | CL_GO_EXT:cell | denotes | cells |
T27006 | 91-96 | CL_GO_EXT:cell | denotes | cells |
T27007 | 123-131 | CHEBI_PR_EXT:collagen | denotes | collagen |
T27008 | 144-156 | GO:0009294 | denotes | transfection |
T27009 | 172-188 | CHEBI_EXT:paraformaldehye | denotes | paraformaldehyde |
T27010 | 192-201 | CHEBI_EXT:phosphate | denotes | phosphate |
T27011 | 202-210 | CHEBI_CHMO_EXT:buffer_process | denotes | buffered |
T27012 | 211-217 | CHEBI_EXT:saline_solution | denotes | saline |
craft-ca-core-dev
Below, discontinuous spans are shown in the chain model. You can change it to the bag model.
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T26982 | 4-15 | GO:0009294 | denotes | transfected |
T26983 | 20-27 | SO:0000155 | denotes | plasmid |
T26984 | 59-63 | PR:000014441 | denotes | mr-s |
T26985 | 144-156 | GO:0009294 | denotes | transfection |
craft-sa-dev
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T27031 | 0-276 | sentence | denotes | We transfected the plasmid encoding HA-tagged full-length mr-s into HEK293T cells, seeded cells on coverslips coated with collagen 48 hr after transfection, fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) at room temperature for 20 min, and washed with PBS. |
T27032 | 1-3 | PRP | denotes | We |
T27033 | 4-15 | VBD | denotes | transfected |
T27034 | 16-19 | DT | denotes | the |
T27035 | 20-27 | NN | denotes | plasmid |
T27036 | 28-36 | VBG | denotes | encoding |
T27037 | 37-39 | NN | denotes | HA |
T27038 | 40-46 | VBN | denotes | tagged |
T27039 | 39-40 | HYPH | denotes | - |
T27040 | 62-63 | NN | denotes | s |
T27041 | 47-51 | JJ | denotes | full |
T27042 | 52-58 | NN | denotes | length |
T27043 | 51-52 | HYPH | denotes | - |
T27044 | 59-61 | NN | denotes | mr |
T27045 | 61-62 | HYPH | denotes | - |
T27046 | 64-68 | IN | denotes | into |
T27047 | 69-76 | NN | denotes | HEK293T |
T27048 | 77-82 | NNS | denotes | cells |
T27049 | 82-84 | , | denotes | , |
T27050 | 84-90 | VBD | denotes | seeded |
T27051 | 91-96 | NNS | denotes | cells |
T27052 | 97-99 | IN | denotes | on |
T27053 | 100-110 | NNS | denotes | coverslips |
T27054 | 111-117 | VBN | denotes | coated |
T27055 | 118-122 | IN | denotes | with |
T27056 | 123-131 | NN | denotes | collagen |
T27057 | 132-134 | CD | denotes | 48 |
T27058 | 135-137 | NN | denotes | hr |
T27059 | 138-143 | IN | denotes | after |
T27060 | 144-156 | NN | denotes | transfection |
T27061 | 156-158 | , | denotes | , |
T27062 | 158-163 | VBN | denotes | fixed |
T27063 | 164-168 | IN | denotes | with |
T27064 | 169-170 | CD | denotes | 4 |
T27065 | 170-171 | NN | denotes | % |
T27066 | 172-188 | NN | denotes | paraformaldehyde |
T27067 | 189-191 | IN | denotes | in |
T27068 | 192-201 | NN | denotes | phosphate |
T27069 | 202-210 | VBN | denotes | buffered |
T27070 | 201-202 | HYPH | denotes | - |
T27071 | 211-217 | NN | denotes | saline |
T27072 | 218-219 | -LRB- | denotes | ( |
T27073 | 219-222 | NN | denotes | PBS |
T27074 | 222-223 | -RRB- | denotes | ) |
T27075 | 224-226 | IN | denotes | at |
T27076 | 227-231 | NN | denotes | room |
T27077 | 232-243 | NN | denotes | temperature |
T27078 | 244-247 | IN | denotes | for |
T27079 | 248-250 | CD | denotes | 20 |
T27080 | 251-254 | NN | denotes | min |
T27081 | 254-256 | , | denotes | , |
T27082 | 256-259 | CC | denotes | and |
T27083 | 260-266 | VBN | denotes | washed |
T27084 | 267-271 | IN | denotes | with |
T27085 | 272-275 | NN | denotes | PBS |
T27086 | 275-276 | . | denotes | . |
R7689 | T27032 | T27033 | nsubj | We,transfected |
R7690 | T27034 | T27035 | det | the,plasmid |
R7691 | T27035 | T27033 | dobj | plasmid,transfected |
R7692 | T27036 | T27035 | acl | encoding,plasmid |
R7693 | T27037 | T27038 | npadvmod | HA,tagged |
R7694 | T27038 | T27040 | amod | tagged,s |
R7695 | T27039 | T27038 | punct | -,tagged |
R7696 | T27040 | T27036 | dobj | s,encoding |
R7697 | T27041 | T27042 | amod | full,length |
R7698 | T27042 | T27040 | compound | length,s |
R7699 | T27043 | T27042 | punct | -,length |
R7700 | T27044 | T27040 | compound | mr,s |
R7701 | T27045 | T27040 | punct | -,s |
R7702 | T27046 | T27033 | prep | into,transfected |
R7703 | T27047 | T27048 | compound | HEK293T,cells |
R7704 | T27048 | T27046 | pobj | cells,into |
R7705 | T27049 | T27033 | punct | ", ",transfected |
R7706 | T27050 | T27033 | conj | seeded,transfected |
R7707 | T27051 | T27050 | dobj | cells,seeded |
R7708 | T27052 | T27050 | prep | on,seeded |
R7709 | T27053 | T27052 | pobj | coverslips,on |
R7710 | T27054 | T27053 | acl | coated,coverslips |
R7711 | T27055 | T27054 | prep | with,coated |
R7712 | T27056 | T27055 | pobj | collagen,with |
R7713 | T27057 | T27058 | nummod | 48,hr |
R7714 | T27058 | T27059 | npadvmod | hr,after |
R7715 | T27059 | T27050 | prep | after,seeded |
R7716 | T27060 | T27059 | pobj | transfection,after |
R7717 | T27061 | T27050 | punct | ", ",seeded |
R7718 | T27062 | T27050 | conj | fixed,seeded |
R7719 | T27063 | T27062 | prep | with,fixed |
R7720 | T27064 | T27065 | nummod | 4,% |
R7721 | T27065 | T27066 | compound | %,paraformaldehyde |
R7722 | T27066 | T27063 | pobj | paraformaldehyde,with |
R7723 | T27067 | T27066 | prep | in,paraformaldehyde |
R7724 | T27068 | T27069 | npadvmod | phosphate,buffered |
R7725 | T27069 | T27071 | amod | buffered,saline |
R7726 | T27070 | T27069 | punct | -,buffered |
R7727 | T27071 | T27067 | pobj | saline,in |
R7728 | T27072 | T27071 | punct | (,saline |
R7729 | T27073 | T27071 | appos | PBS,saline |
R7730 | T27074 | T27062 | punct | ),fixed |
R7731 | T27075 | T27062 | prep | at,fixed |
R7732 | T27076 | T27077 | compound | room,temperature |
R7733 | T27077 | T27075 | pobj | temperature,at |
R7734 | T27078 | T27062 | prep | for,fixed |
R7735 | T27079 | T27080 | nummod | 20,min |
R7736 | T27080 | T27078 | pobj | min,for |
R7737 | T27081 | T27062 | punct | ", ",fixed |
R7738 | T27082 | T27062 | cc | and,fixed |
R7739 | T27083 | T27062 | conj | washed,fixed |
R7740 | T27084 | T27083 | prep | with,washed |
R7741 | T27085 | T27084 | pobj | PBS,with |
R7742 | T27086 | T27033 | punct | .,transfected |