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PMC:1435744 / 44340-46184 JSONTXT

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craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T26130 64-70 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T26131 71-75 SO_EXT:cDNA denotes cDNA
T26132 85-93 SO_EXT:sequence_coding_function denotes encoding
T26133 106-110 PR_EXT:000014441 denotes mr-s
T26134 129-132 PR_EXT:000014441 denotes mrs
T26135 137-141 SO_EXT:cDNA denotes cDNA
T26136 151-159 SO_EXT:sequence_coding_function denotes encoding
T26137 160-171 CHEBI_SO_EXT:amino_acid denotes amino acids
T26138 209-213 SO_EXT:cDNA denotes cDNA
T26139 223-231 SO_EXT:sequence_coding_function denotes encoding
T26140 232-243 CHEBI_SO_EXT:amino_acid denotes amino acids
T26141 307-317 GO:0010467 denotes expression
T26142 318-324 SO_EXT:0000440 denotes vector
T26143 355-363 GO_EXT:biological_direction_or_guidance denotes directed
T26144 364-371 SO_EXT:sequence_altered_entity denotes mutants
T26145 425-434 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T26146 465-469 PR_EXT:000014441 denotes mr-s
T26147 474-485 GO:0009294 denotes transfected
T26148 494-499 CL_GO_EXT:cell denotes cells
T26149 513-520 SO_EXT:0000155 denotes plasmid
T26150 521-524 CHEBI_SO_EXT:DNA denotes DNA
T26151 542-559 CHEBI:77635 denotes calcium phosphate
T26152 594-606 GO:0009294 denotes transfection
T26153 608-613 CL_GO_EXT:cell denotes cells
T26154 652-658 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T26155 666-670 CHEBI:9754 denotes Tris
T26156 671-674 CHEBI:17883 denotes HCl
T26157 690-694 CHEBI_EXT:EDTA denotes EDTA
T26158 701-706 CHEBI:6636 denotes MgCl2
T26159 715-719 CHEBI:26710 denotes NaCl
T26160 726-755 CHEBI:8102 denotes phenylmethylsulfonyl fluoride
T26161 767-779 CHEBI:78708 denotes Nonidet P-40
T26162 785-793 CHEBI:17754 denotes glycerol
T26163 814-832 CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor denotes protease inhibitor
T26164 833-841 CHEBI:60004 denotes cocktail
T26165 886-890 CL_GO_EXT:cell denotes cell
T26166 933-941 GO:0042571 denotes antibody
T26167 970-977 CHEBI_PR_EXT:protein denotes protein
T26168 1039-1046 CHEBI_PR_EXT:protein denotes Protein
T26169 1087-1094 CHEBI_PR_EXT:protein denotes protein
T26170 1182-1188 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T26171 1227-1233 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T26172 1241-1245 CHEBI:9754 denotes Tris
T26173 1246-1249 CHEBI:17883 denotes HCl
T26174 1267-1271 CHEBI:26710 denotes NaCl
T26175 1279-1284 CHEBI:6636 denotes MgCl2
T26176 1287-1295 CHEBI_PR_EXT:protein denotes Proteins
T26177 1326-1329 CHEBI:8984 denotes SDS
T26178 1337-1343 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T26179 1348-1351 CHEBI:8984 denotes SDS
T26180 1358-1362 CHEBI:9754 denotes Tris
T26181 1379-1382 CHEBI:18320 denotes DTT
T26182 1387-1395 CHEBI:17754 denotes glycerol
T26183 1403-1413 CHEBI:8682 denotes pyronine Y
T26184 1454-1476 CHEBI:8984 denotes sodium dodecyl sulfate
T26185 1477-1491 CHEBI_EXT:polyacrylamide denotes polyacrylamide
T26186 1513-1516 CHEBI:8984 denotes SDS
T26187 1527-1538 GO_EXT:biological_movement_or_translocation_process denotes transferred
T26188 1546-1560 CHEBI:53325 denotes nitrocellulose
T26189 1582-1590 GO_EXT:biological_movement_or_translocation_process denotes Transfer
T26190 1645-1651 NCBITaxon:9986 denotes rabbit
T26191 1671-1679 GO:0042571 denotes antibody
T26192 1730-1741 NCBITaxon:3704 denotes horseradish
T26193 1742-1752 GO_EXT:0004601 denotes peroxidase
T26194 1754-1764 MOP:0000779 denotes conjugated
T26195 1765-1769 NCBITaxon:9925 denotes goat
T26196 1775-1781 NCBITaxon:9986 denotes rabbit
T26197 1782-1785 GO:0071735 denotes IgG

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T26092 106-110 PR:000014441 denotes mr-s
T26093 129-132 PR:000014441 denotes mrs
T26094 307-317 GO:0010467 denotes expression
T26095 318-324 SO:0000440 denotes vector
T26096 465-469 PR:000014441 denotes mr-s
T26097 474-485 GO:0009294 denotes transfected
T26098 513-520 SO:0000155 denotes plasmid
T26099 542-559 CHEBI:77635 denotes calcium phosphate
T26100 594-606 GO:0009294 denotes transfection
T26101 666-670 CHEBI:9754 denotes Tris
T26102 671-674 CHEBI:17883 denotes HCl
T26103 701-706 CHEBI:6636 denotes MgCl2
T26104 715-719 CHEBI:26710 denotes NaCl
T26105 726-755 CHEBI:8102 denotes phenylmethylsulfonyl fluoride
T26106 767-779 CHEBI:78708 denotes Nonidet P-40
T26107 785-793 CHEBI:17754 denotes glycerol
T26108 833-841 CHEBI:60004 denotes cocktail
T26109 933-941 GO:0042571 denotes antibody
T26110 1241-1245 CHEBI:9754 denotes Tris
T26111 1246-1249 CHEBI:17883 denotes HCl
T26112 1267-1271 CHEBI:26710 denotes NaCl
T26113 1279-1284 CHEBI:6636 denotes MgCl2
T26114 1326-1329 CHEBI:8984 denotes SDS
T26115 1348-1351 CHEBI:8984 denotes SDS
T26116 1358-1362 CHEBI:9754 denotes Tris
T26117 1379-1382 CHEBI:18320 denotes DTT
T26118 1387-1395 CHEBI:17754 denotes glycerol
T26119 1403-1413 CHEBI:8682 denotes pyronine Y
T26120 1454-1476 CHEBI:8984 denotes sodium dodecyl sulfate
T26121 1513-1516 CHEBI:8984 denotes SDS
T26122 1546-1560 CHEBI:53325 denotes nitrocellulose
T26123 1645-1651 NCBITaxon:9986 denotes rabbit
T26124 1671-1679 GO:0042571 denotes antibody
T26125 1730-1741 NCBITaxon:3704 denotes horseradish
T26126 1754-1764 MOP:0000779 denotes conjugated
T26127 1765-1769 NCBITaxon:9925 denotes goat
T26128 1775-1781 NCBITaxon:9986 denotes rabbit
T26129 1782-1785 GO:0071735 denotes IgG

craft-sa-dev

Id Subject Object Predicate Lexical cue
T26201 0-3 IN denotes For
T26202 268-277 VBN denotes subcloned
T26203 4-23 NN denotes immunoprecipitation
T26204 24-29 NN denotes assay
T26205 29-31 , denotes ,
T26206 31-32 CD denotes 4
T26207 34-47 NN denotes hemagglutinin
T26208 32-33 SYM denotes ×
T26209 64-70 VBN denotes tagged
T26210 48-49 -LRB- denotes (
T26211 49-51 NN denotes HA
T26212 51-52 -RRB- denotes )
T26213 53-55 CC denotes or
T26214 56-57 CD denotes 3
T26215 59-63 NN denotes Flag
T26216 57-58 SYM denotes ×
T26217 76-84 NN denotes fragment
T26218 71-75 NN denotes cDNA
T26219 85-93 VBG denotes encoding
T26220 94-98 JJ denotes full
T26221 99-105 NN denotes length
T26222 98-99 HYPH denotes -
T26223 109-110 NN denotes s
T26224 106-108 NN denotes mr
T26225 108-109 HYPH denotes -
T26226 111-112 -LRB- denotes (
T26227 117-119 NN denotes HA
T26228 112-116 JJ denotes full
T26229 116-117 HYPH denotes -
T26230 120-123 CC denotes and
T26231 124-128 NN denotes Flag
T26232 129-132 NN denotes mrs
T26233 128-129 HYPH denotes -
T26234 132-133 -RRB- denotes )
T26235 133-135 , denotes ,
T26236 135-136 DT denotes a
T26237 142-150 NN denotes fragment
T26238 137-141 NN denotes cDNA
T26239 151-159 VBG denotes encoding
T26240 160-165 NN denotes amino
T26241 172-173 CD denotes 1
T26242 166-171 NNS denotes acids
T26243 173-174 SYM denotes –
T26244 174-177 CD denotes 400
T26245 178-179 -LRB- denotes (
T26246 184-186 NN denotes HA
T26247 179-183 NN denotes ΔSAM
T26248 183-184 HYPH denotes -
T26249 187-190 CC denotes and
T26250 191-195 NN denotes Flag
T26251 196-200 NN denotes ΔSAM
T26252 195-196 HYPH denotes -
T26253 200-201 -RRB- denotes )
T26254 201-203 , denotes ,
T26255 203-206 CC denotes and
T26256 207-208 DT denotes a
T26257 214-222 NN denotes fragment
T26258 209-213 NN denotes cDNA
T26259 223-231 VBG denotes encoding
T26260 232-237 NN denotes amino
T26261 244-247 CD denotes 400
T26262 238-243 NNS denotes acids
T26263 247-248 SYM denotes –
T26264 248-251 CD denotes 542
T26265 252-253 -LRB- denotes (
T26266 258-261 NN denotes SAM
T26267 253-257 NN denotes Flag
T26268 257-258 HYPH denotes -
T26269 261-262 -RRB- denotes )
T26270 263-267 VBD denotes were
T26271 278-282 IN denotes into
T26272 283-286 DT denotes the
T26273 318-324 NN denotes vector
T26274 287-293 NN denotes pcDNA3
T26275 294-295 -LRB- denotes (
T26276 295-305 NNP denotes Invitrogen
T26277 305-306 -RRB- denotes )
T26278 307-317 NN denotes expression
T26279 324-325 . denotes .
T26280 325-410 sentence denotes We also constructed two site-directed mutants, Flag-W404A and Flag-G453A, using PCR.
T26281 326-328 PRP denotes We
T26282 334-345 VBD denotes constructed
T26283 329-333 RB denotes also
T26284 346-349 CD denotes two
T26285 364-371 NNS denotes mutants
T26286 350-354 NN denotes site
T26287 355-363 VBN denotes directed
T26288 354-355 HYPH denotes -
T26289 371-373 , denotes ,
T26290 373-377 NN denotes Flag
T26291 378-383 NN denotes W404A
T26292 377-378 HYPH denotes -
T26293 384-387 CC denotes and
T26294 388-392 NN denotes Flag
T26295 393-398 NN denotes G453A
T26296 392-393 HYPH denotes -
T26297 398-400 , denotes ,
T26298 400-405 VBG denotes using
T26299 406-409 NN denotes PCR
T26300 409-410 . denotes .
T26301 410-470 sentence denotes Each of these mutations was also introduced into Flag-mr-s.
T26302 411-415 DT denotes Each
T26303 444-454 VBN denotes introduced
T26304 416-418 IN denotes of
T26305 419-424 DT denotes these
T26306 425-434 NNS denotes mutations
T26307 435-438 VBD denotes was
T26308 439-443 RB denotes also
T26309 455-459 IN denotes into
T26310 460-464 NN denotes Flag
T26311 464-465 HYPH denotes -
T26312 465-467 NN denotes mr
T26313 468-469 NN denotes s
T26314 467-468 HYPH denotes -
T26315 469-470 . denotes .
T26316 470-567 sentence denotes We transfected HEK293T cells with 5 μg of plasmid DNA per 6 cm dish by calcium phosphate method.
T26317 471-473 PRP denotes We
T26318 474-485 VBD denotes transfected
T26319 486-493 NN denotes HEK293T
T26320 494-499 NNS denotes cells
T26321 500-504 IN denotes with
T26322 505-506 CD denotes 5
T26323 507-509 NN denotes μg
T26324 510-512 IN denotes of
T26325 513-520 NN denotes plasmid
T26326 521-524 NN denotes DNA
T26327 525-528 IN denotes per
T26328 529-530 CD denotes 6
T26329 531-533 NN denotes cm
T26330 534-538 NN denotes dish
T26331 539-541 IN denotes by
T26332 542-549 NN denotes calcium
T26333 550-559 NN denotes phosphate
T26334 560-566 NN denotes method
T26335 566-567 . denotes .
T26336 567-858 sentence denotes Approximately 48 hr after transfection, cells were harvested in immunoprecipitation buffer (50 mM Tris-HCl [pH 7.5], 1 mM EDTA, 2 mM MgCl2, 150 mM NaCl, 1 mM phenylmethylsulfonyl fluoride [Wako], 1% Nonidet P-40, 10% glycerol) in the presence of protease inhibitor cocktail tablets (Roche).
T26337 568-581 RB denotes Approximately
T26338 582-584 CD denotes 48
T26339 585-587 NN denotes hr
T26340 588-593 IN denotes after
T26341 619-628 VBN denotes harvested
T26342 594-606 NN denotes transfection
T26343 606-608 , denotes ,
T26344 608-613 NNS denotes cells
T26345 614-618 VBD denotes were
T26346 629-631 IN denotes in
T26347 632-651 NN denotes immunoprecipitation
T26348 652-658 NN denotes buffer
T26349 659-660 -LRB- denotes (
T26350 671-674 NN denotes HCl
T26351 660-662 CD denotes 50
T26352 663-665 NN denotes mM
T26353 666-670 NN denotes Tris
T26354 670-671 HYPH denotes -
T26355 675-676 -LRB- denotes [
T26356 676-678 NN denotes pH
T26357 679-682 CD denotes 7.5
T26358 682-683 -RRB- denotes ]
T26359 683-685 , denotes ,
T26360 685-686 CD denotes 1
T26361 687-689 NN denotes mM
T26362 690-694 NN denotes EDTA
T26363 694-696 , denotes ,
T26364 696-697 CD denotes 2
T26365 698-700 NN denotes mM
T26366 701-706 NN denotes MgCl2
T26367 706-708 , denotes ,
T26368 708-711 CD denotes 150
T26369 712-714 NN denotes mM
T26370 715-719 NN denotes NaCl
T26371 719-721 , denotes ,
T26372 721-722 CD denotes 1
T26373 723-725 NN denotes mM
T26374 747-755 NN denotes fluoride
T26375 726-746 NN denotes phenylmethylsulfonyl
T26376 756-757 -LRB- denotes [
T26377 757-761 NN denotes Wako
T26378 761-762 -RRB- denotes ]
T26379 762-764 , denotes ,
T26380 764-765 CD denotes 1
T26381 765-766 NN denotes %
T26382 775-776 NN denotes P
T26383 767-774 NN denotes Nonidet
T26384 776-777 HYPH denotes -
T26385 777-779 CD denotes 40
T26386 779-781 , denotes ,
T26387 781-783 CD denotes 10
T26388 783-784 NN denotes %
T26389 785-793 NN denotes glycerol
T26390 793-794 -RRB- denotes )
T26391 795-797 IN denotes in
T26392 798-801 DT denotes the
T26393 802-810 NN denotes presence
T26394 811-813 IN denotes of
T26395 814-822 NN denotes protease
T26396 823-832 NN denotes inhibitor
T26397 842-849 NNS denotes tablets
T26398 833-841 NN denotes cocktail
T26399 850-851 -LRB- denotes (
T26400 851-856 NNP denotes Roche
T26401 856-857 -RRB- denotes )
T26402 857-858 . denotes .
T26403 858-1038 sentence denotes For each reaction, 1 mg of cell lysate was mixed with 0.5 μg of anti-Flag antibody (SIGMA, F3165) and 15 μl of protein G-Sepharose (Amersham) on a rotating wheel at 4°C for 2 hrs.
T26404 859-862 IN denotes For
T26405 902-907 VBN denotes mixed
T26406 863-867 DT denotes each
T26407 868-876 NN denotes reaction
T26408 876-878 , denotes ,
T26409 878-879 CD denotes 1
T26410 880-882 NN denotes mg
T26411 883-885 IN denotes of
T26412 886-890 NN denotes cell
T26413 891-897 NN denotes lysate
T26414 898-901 VBD denotes was
T26415 908-912 IN denotes with
T26416 913-916 CD denotes 0.5
T26417 917-919 NN denotes μg
T26418 920-922 IN denotes of
T26419 923-932 JJ denotes anti-Flag
T26420 933-941 NN denotes antibody
T26421 942-943 -LRB- denotes (
T26422 950-955 NN denotes F3165
T26423 943-948 NNP denotes SIGMA
T26424 948-950 , denotes ,
T26425 955-956 -RRB- denotes )
T26426 957-960 CC denotes and
T26427 961-963 CD denotes 15
T26428 964-966 NN denotes μl
T26429 967-969 IN denotes of
T26430 970-977 NN denotes protein
T26431 980-989 NN denotes Sepharose
T26432 978-979 NN denotes G
T26433 979-980 HYPH denotes -
T26434 990-991 -LRB- denotes (
T26435 991-999 NNP denotes Amersham
T26436 999-1000 -RRB- denotes )
T26437 1001-1003 IN denotes on
T26438 1004-1005 DT denotes a
T26439 1015-1020 NN denotes wheel
T26440 1006-1014 VBG denotes rotating
T26441 1021-1023 IN denotes at
T26442 1024-1025 CD denotes 4
T26443 1025-1027 NN denotes °C
T26444 1028-1031 IN denotes for
T26445 1032-1033 CD denotes 2
T26446 1034-1037 NNS denotes hrs
T26447 1037-1038 . denotes .
T26448 1038-1117 sentence denotes Protein concentration was determined by the BCA protein assay system (Pierce).
T26449 1039-1046 NN denotes Protein
T26450 1047-1060 NN denotes concentration
T26451 1065-1075 VBN denotes determined
T26452 1061-1064 VBD denotes was
T26453 1076-1078 IN denotes by
T26454 1079-1082 DT denotes the
T26455 1101-1107 NN denotes system
T26456 1083-1086 NN denotes BCA
T26457 1095-1100 NN denotes assay
T26458 1087-1094 NN denotes protein
T26459 1108-1109 -LRB- denotes (
T26460 1109-1115 NNP denotes Pierce
T26461 1115-1116 -RRB- denotes )
T26462 1116-1117 . denotes .
T26463 1117-1286 sentence denotes The beads were then washed three times with immunoprecipitation buffer and followed by three times with wash buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 20 mM MgCl2).
T26464 1118-1121 DT denotes The
T26465 1122-1127 NNS denotes beads
T26466 1138-1144 VBN denotes washed
T26467 1128-1132 VBD denotes were
T26468 1133-1137 RB denotes then
T26469 1145-1150 CD denotes three
T26470 1151-1156 NNS denotes times
T26471 1157-1161 IN denotes with
T26472 1162-1181 NN denotes immunoprecipitation
T26473 1182-1188 NN denotes buffer
T26474 1189-1192 CC denotes and
T26475 1193-1201 VBN denotes followed
T26476 1202-1204 IN denotes by
T26477 1205-1210 CD denotes three
T26478 1211-1216 NNS denotes times
T26479 1217-1221 IN denotes with
T26480 1222-1226 NN denotes wash
T26481 1227-1233 NN denotes buffer
T26482 1234-1235 -LRB- denotes (
T26483 1246-1249 NN denotes HCl
T26484 1235-1237 CD denotes 50
T26485 1238-1240 NN denotes mM
T26486 1241-1245 NN denotes Tris
T26487 1245-1246 HYPH denotes -
T26488 1250-1251 -LRB- denotes [
T26489 1251-1253 NN denotes pH
T26490 1254-1257 CD denotes 7.5
T26491 1257-1258 -RRB- denotes ]
T26492 1258-1260 , denotes ,
T26493 1260-1263 CD denotes 150
T26494 1264-1266 NN denotes mM
T26495 1267-1271 NN denotes NaCl
T26496 1271-1273 , denotes ,
T26497 1273-1275 CD denotes 20
T26498 1276-1278 NN denotes mM
T26499 1279-1284 NN denotes MgCl2
T26500 1284-1285 -RRB- denotes )
T26501 1285-1286 . denotes .
T26502 1286-1415 sentence denotes Proteins were then boiled for 5 min in SDS sample buffer (1% SDS, 1 mM Tris [pH 6.8], 40 mM DTT, 4% glycerol, 0.01% pyronine Y).
T26503 1287-1295 NNS denotes Proteins
T26504 1306-1312 VBN denotes boiled
T26505 1296-1300 VBD denotes were
T26506 1301-1305 RB denotes then
T26507 1313-1316 IN denotes for
T26508 1317-1318 CD denotes 5
T26509 1319-1322 NN denotes min
T26510 1323-1325 IN denotes in
T26511 1326-1329 NN denotes SDS
T26512 1337-1343 NN denotes buffer
T26513 1330-1336 NN denotes sample
T26514 1344-1345 -LRB- denotes (
T26515 1348-1351 NN denotes SDS
T26516 1345-1346 CD denotes 1
T26517 1346-1347 NN denotes %
T26518 1351-1353 , denotes ,
T26519 1353-1354 CD denotes 1
T26520 1355-1357 NN denotes mM
T26521 1358-1362 NN denotes Tris
T26522 1363-1364 -LRB- denotes [
T26523 1364-1366 NN denotes pH
T26524 1367-1370 CD denotes 6.8
T26525 1370-1371 -RRB- denotes ]
T26526 1371-1373 , denotes ,
T26527 1373-1375 CD denotes 40
T26528 1376-1378 NN denotes mM
T26529 1379-1382 NN denotes DTT
T26530 1382-1384 , denotes ,
T26531 1384-1385 CD denotes 4
T26532 1385-1386 NN denotes %
T26533 1387-1395 NN denotes glycerol
T26534 1395-1397 , denotes ,
T26535 1397-1401 CD denotes 0.01
T26536 1401-1402 NN denotes %
T26537 1412-1413 NN denotes Y
T26538 1403-1411 NN denotes pyronine
T26539 1413-1414 -RRB- denotes )
T26540 1414-1415 . denotes .
T26541 1415-1608 sentence denotes The supernatants were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to the nitrocellulose membrane (Trans-Blot Transfer Medium, Bio-Rad).
T26542 1416-1419 DT denotes The
T26543 1420-1432 NNS denotes supernatants
T26544 1438-1450 VBN denotes fractionated
T26545 1433-1437 VBD denotes were
T26546 1451-1453 IN denotes by
T26547 1454-1460 NN denotes sodium
T26548 1469-1476 NN denotes sulfate
T26549 1461-1468 NN denotes dodecyl
T26550 1492-1495 NN denotes gel
T26551 1476-1477 HYPH denotes -
T26552 1477-1491 NN denotes polyacrylamide
T26553 1496-1511 NN denotes electrophoresis
T26554 1512-1513 -LRB- denotes (
T26555 1517-1521 NN denotes PAGE
T26556 1513-1516 NN denotes SDS
T26557 1516-1517 HYPH denotes -
T26558 1521-1522 -RRB- denotes )
T26559 1523-1526 CC denotes and
T26560 1527-1538 VBN denotes transferred
T26561 1539-1541 IN denotes to
T26562 1542-1545 DT denotes the
T26563 1561-1569 NN denotes membrane
T26564 1546-1560 NN denotes nitrocellulose
T26565 1570-1571 -LRB- denotes (
T26566 1591-1597 NN denotes Medium
T26567 1571-1576 NN denotes Trans
T26568 1577-1581 NN denotes Blot
T26569 1576-1577 HYPH denotes -
T26570 1582-1590 NN denotes Transfer
T26571 1597-1599 , denotes ,
T26572 1599-1602 NNP denotes Bio
T26573 1603-1606 NNP denotes Rad
T26574 1602-1603 HYPH denotes -
T26575 1606-1607 -RRB- denotes )
T26576 1607-1608 . denotes .
T26577 1608-1702 sentence denotes Western blotting was performed with rabbit polyclonal anti-HA antibody (Santa Cruz, #sc-805).
T26578 1609-1616 NNP denotes Western
T26579 1617-1625 NN denotes blotting
T26580 1630-1639 VBN denotes performed
T26581 1626-1629 VBD denotes was
T26582 1640-1644 IN denotes with
T26583 1645-1651 NN denotes rabbit
T26584 1671-1679 NN denotes antibody
T26585 1652-1662 JJ denotes polyclonal
T26586 1663-1670 JJ denotes anti-HA
T26587 1680-1681 -LRB- denotes (
T26588 1687-1691 NNP denotes Cruz
T26589 1681-1686 NNP denotes Santa
T26590 1691-1693 , denotes ,
T26591 1693-1694 SYM denotes #
T26592 1694-1696 NN denotes sc
T26593 1696-1697 HYPH denotes -
T26594 1697-1700 CD denotes 805
T26595 1700-1701 -RRB- denotes )
T26596 1701-1702 . denotes .
T26597 1702-1844 sentence denotes Signals were detected with horseradish peroxidase- conjugated goat anti-rabbit IgG and ECL plus Western Blotting Detection System (Amersham).
T26598 1703-1710 NNS denotes Signals
T26599 1716-1724 VBN denotes detected
T26600 1711-1715 VBD denotes were
T26601 1725-1729 IN denotes with
T26602 1730-1741 NN denotes horseradish
T26603 1742-1752 NN denotes peroxidase
T26604 1754-1764 VBN denotes conjugated
T26605 1752-1753 HYPH denotes -
T26606 1782-1785 NN denotes IgG
T26607 1765-1769 NN denotes goat
T26608 1770-1781 JJ denotes anti-rabbit
T26609 1786-1789 CC denotes and
T26610 1790-1793 NN denotes ECL
T26611 1826-1832 NN denotes System
T26612 1794-1798 CC denotes plus
T26613 1799-1806 NNP denotes Western
T26614 1807-1815 NN denotes Blotting
T26615 1816-1825 NN denotes Detection
T26616 1833-1834 -LRB- denotes (
T26617 1834-1842 NNP denotes Amersham
T26618 1842-1843 -RRB- denotes )
T26619 1843-1844 . denotes .
R7291 T26201 T26202 prep For,subcloned
R7292 T26203 T26204 compound immunoprecipitation,assay
R7293 T26204 T26201 pobj assay,For
R7294 T26205 T26202 punct ", ",subcloned
R7295 T26206 T26207 nummod 4,hemagglutinin
R7296 T26207 T26209 npadvmod hemagglutinin,tagged
R7297 T26208 T26206 punct ×,4
R7298 T26209 T26217 amod tagged,fragment
R7299 T26210 T26207 punct (,hemagglutinin
R7300 T26211 T26207 appos HA,hemagglutinin
R7301 T26212 T26207 punct ),hemagglutinin
R7302 T26213 T26207 cc or,hemagglutinin
R7303 T26214 T26215 nummod 3,Flag
R7304 T26215 T26207 conj Flag,hemagglutinin
R7305 T26216 T26214 punct ×,3
R7306 T26217 T26202 nsubjpass fragment,subcloned
R7307 T26218 T26217 compound cDNA,fragment
R7308 T26219 T26217 acl encoding,fragment
R7309 T26220 T26221 amod full,length
R7310 T26221 T26223 compound length,s
R7311 T26222 T26221 punct -,length
R7312 T26223 T26219 dobj s,encoding
R7313 T26224 T26223 compound mr,s
R7314 T26225 T26223 punct -,s
R7315 T26226 T26227 punct (,HA
R7316 T26227 T26223 parataxis HA,s
R7317 T26228 T26227 amod full,HA
R7318 T26229 T26227 punct -,HA
R7319 T26230 T26227 cc and,HA
R7320 T26231 T26232 compound Flag,mrs
R7321 T26232 T26227 conj mrs,HA
R7322 T26233 T26232 punct -,mrs
R7323 T26234 T26227 punct ),HA
R7324 T26235 T26223 punct ", ",s
R7325 T26236 T26237 det a,fragment
R7326 T26237 T26223 conj fragment,s
R7327 T26238 T26237 compound cDNA,fragment
R7328 T26239 T26237 acl encoding,fragment
R7329 T26240 T26241 nmod amino,1
R7330 T26241 T26239 dobj 1,encoding
R7331 T26242 T26241 nmod acids,1
R7332 T26243 T26244 punct –,400
R7333 T26244 T26241 prep 400,1
R7334 T26245 T26246 punct (,HA
R7335 T26246 T26237 parataxis HA,fragment
R7336 T26247 T26246 compound ΔSAM,HA
R7337 T26248 T26246 punct -,HA
R7338 T26249 T26246 cc and,HA
R7339 T26250 T26251 compound Flag,ΔSAM
R7340 T26251 T26246 conj ΔSAM,HA
R7341 T26252 T26251 punct -,ΔSAM
R7342 T26253 T26246 punct ),HA
R7343 T26254 T26237 punct ", ",fragment
R7344 T26255 T26237 cc and,fragment
R7345 T26256 T26257 det a,fragment
R7346 T26257 T26237 conj fragment,fragment
R7347 T26258 T26257 compound cDNA,fragment
R7348 T26259 T26257 acl encoding,fragment
R7349 T26260 T26261 nmod amino,400
R7350 T26261 T26259 dobj 400,encoding
R7351 T26262 T26261 nmod acids,400
R7352 T26263 T26264 punct –,542
R7353 T26264 T26261 prep 542,400
R7354 T26265 T26266 punct (,SAM
R7355 T26266 T26257 parataxis SAM,fragment
R7356 T26267 T26266 compound Flag,SAM
R7357 T26268 T26266 punct -,SAM
R7358 T26269 T26266 punct ),SAM
R7359 T26270 T26202 auxpass were,subcloned
R7360 T26271 T26202 prep into,subcloned
R7361 T26272 T26273 det the,vector
R7362 T26273 T26271 pobj vector,into
R7363 T26274 T26273 nmod pcDNA3,vector
R7364 T26275 T26276 punct (,Invitrogen
R7365 T26276 T26274 parataxis Invitrogen,pcDNA3
R7366 T26277 T26276 punct ),Invitrogen
R7367 T26278 T26273 compound expression,vector
R7368 T26279 T26202 punct .,subcloned
R7369 T26281 T26282 nsubj We,constructed
R7370 T26283 T26282 advmod also,constructed
R7371 T26284 T26285 nummod two,mutants
R7372 T26285 T26282 dobj mutants,constructed
R7373 T26286 T26287 npadvmod site,directed
R7374 T26287 T26285 amod directed,mutants
R7375 T26288 T26287 punct -,directed
R7376 T26289 T26285 punct ", ",mutants
R7377 T26290 T26291 compound Flag,W404A
R7378 T26291 T26285 appos W404A,mutants
R7379 T26292 T26291 punct -,W404A
R7380 T26293 T26291 cc and,W404A
R7381 T26294 T26295 compound Flag,G453A
R7382 T26295 T26291 conj G453A,W404A
R7383 T26296 T26295 punct -,G453A
R7384 T26297 T26282 punct ", ",constructed
R7385 T26298 T26282 advcl using,constructed
R7386 T26299 T26298 dobj PCR,using
R7387 T26300 T26282 punct .,constructed
R7388 T26302 T26303 nsubjpass Each,introduced
R7389 T26304 T26302 prep of,Each
R7390 T26305 T26306 det these,mutations
R7391 T26306 T26304 pobj mutations,of
R7392 T26307 T26303 auxpass was,introduced
R7393 T26308 T26303 advmod also,introduced
R7394 T26309 T26303 prep into,introduced
R7395 T26310 T26309 pobj Flag,into
R7396 T26311 T26310 punct -,Flag
R7397 T26312 T26313 compound mr,s
R7398 T26313 T26310 appos s,Flag
R7399 T26314 T26313 punct -,s
R7400 T26315 T26303 punct .,introduced
R7401 T26317 T26318 nsubj We,transfected
R7402 T26319 T26320 compound HEK293T,cells
R7403 T26320 T26318 dobj cells,transfected
R7404 T26321 T26318 prep with,transfected
R7405 T26322 T26323 nummod 5,μg
R7406 T26323 T26321 pobj μg,with
R7407 T26324 T26323 prep of,μg
R7408 T26325 T26326 compound plasmid,DNA
R7409 T26326 T26324 pobj DNA,of
R7410 T26327 T26323 prep per,μg
R7411 T26328 T26329 nummod 6,cm
R7412 T26329 T26330 compound cm,dish
R7413 T26330 T26327 pobj dish,per
R7414 T26331 T26318 prep by,transfected
R7415 T26332 T26333 compound calcium,phosphate
R7416 T26333 T26334 compound phosphate,method
R7417 T26334 T26331 pobj method,by
R7418 T26335 T26318 punct .,transfected
R7419 T26337 T26338 advmod Approximately,48
R7420 T26338 T26339 nummod 48,hr
R7421 T26339 T26340 npadvmod hr,after
R7422 T26340 T26341 prep after,harvested
R7423 T26342 T26340 pobj transfection,after
R7424 T26343 T26341 punct ", ",harvested
R7425 T26344 T26341 nsubjpass cells,harvested
R7426 T26345 T26341 auxpass were,harvested
R7427 T26346 T26341 prep in,harvested
R7428 T26347 T26348 compound immunoprecipitation,buffer
R7429 T26348 T26346 pobj buffer,in
R7430 T26349 T26350 punct (,HCl
R7431 T26350 T26341 parataxis HCl,harvested
R7432 T26351 T26352 nummod 50,mM
R7433 T26352 T26350 compound mM,HCl
R7434 T26353 T26350 compound Tris,HCl
R7435 T26354 T26350 punct -,HCl
R7436 T26355 T26356 punct [,pH
R7437 T26356 T26350 parataxis pH,HCl
R7438 T26357 T26356 nummod 7.5,pH
R7439 T26358 T26356 punct ],pH
R7440 T26359 T26350 punct ", ",HCl
R7441 T26360 T26361 nummod 1,mM
R7442 T26361 T26362 compound mM,EDTA
R7443 T26362 T26350 appos EDTA,HCl
R7444 T26363 T26350 punct ", ",HCl
R7445 T26364 T26365 nummod 2,mM
R7446 T26365 T26366 compound mM,MgCl2
R7447 T26366 T26350 appos MgCl2,HCl
R7448 T26367 T26350 punct ", ",HCl
R7449 T26368 T26369 nummod 150,mM
R7450 T26369 T26370 compound mM,NaCl
R7451 T26370 T26350 appos NaCl,HCl
R7452 T26371 T26350 punct ", ",HCl
R7453 T26372 T26373 nummod 1,mM
R7454 T26373 T26374 compound mM,fluoride
R7455 T26374 T26350 appos fluoride,HCl
R7456 T26375 T26374 compound phenylmethylsulfonyl,fluoride
R7457 T26376 T26377 punct [,Wako
R7458 T26377 T26374 parataxis Wako,fluoride
R7459 T26378 T26377 punct ],Wako
R7460 T26379 T26350 punct ", ",HCl
R7461 T26380 T26381 nummod 1,%
R7462 T26381 T26382 compound %,P
R7463 T26382 T26350 appos P,HCl
R7464 T26383 T26382 compound Nonidet,P
R7465 T26384 T26382 punct -,P
R7466 T26385 T26382 nummod 40,P
R7467 T26386 T26350 punct ", ",HCl
R7468 T26387 T26388 nummod 10,%
R7469 T26388 T26389 compound %,glycerol
R7470 T26389 T26350 appos glycerol,HCl
R7471 T26390 T26350 punct ),HCl
R7472 T26391 T26341 prep in,harvested
R7473 T26392 T26393 det the,presence
R7474 T26393 T26391 pobj presence,in
R7475 T26394 T26393 prep of,presence
R7476 T26395 T26396 compound protease,inhibitor
R7477 T26396 T26397 compound inhibitor,tablets
R7478 T26397 T26394 pobj tablets,of
R7479 T26398 T26397 compound cocktail,tablets
R7480 T26399 T26400 punct (,Roche
R7481 T26400 T26397 parataxis Roche,tablets
R7482 T26401 T26400 punct ),Roche
R7483 T26402 T26341 punct .,harvested
R7484 T26404 T26405 prep For,mixed
R7485 T26406 T26407 det each,reaction
R7486 T26407 T26404 pobj reaction,For
R7487 T26408 T26405 punct ", ",mixed
R7488 T26409 T26410 nummod 1,mg
R7489 T26410 T26405 nsubjpass mg,mixed
R7490 T26411 T26410 prep of,mg
R7491 T26412 T26413 compound cell,lysate
R7492 T26413 T26411 pobj lysate,of
R7493 T26414 T26405 auxpass was,mixed
R7494 T26415 T26405 prep with,mixed
R7495 T26416 T26417 nummod 0.5,μg
R7496 T26417 T26415 pobj μg,with
R7497 T26418 T26417 prep of,μg
R7498 T26419 T26420 amod anti-Flag,antibody
R7499 T26420 T26418 pobj antibody,of
R7500 T26421 T26422 punct (,F3165
R7501 T26422 T26420 parataxis F3165,antibody
R7502 T26423 T26422 nmod SIGMA,F3165
R7503 T26424 T26422 punct ", ",F3165
R7504 T26425 T26422 punct ),F3165
R7505 T26426 T26417 cc and,μg
R7506 T26427 T26428 nummod 15,μl
R7507 T26428 T26417 conj μl,μg
R7508 T26429 T26428 prep of,μl
R7509 T26430 T26431 compound protein,Sepharose
R7510 T26431 T26429 pobj Sepharose,of
R7511 T26432 T26431 compound G,Sepharose
R7512 T26433 T26431 punct -,Sepharose
R7513 T26434 T26435 punct (,Amersham
R7514 T26435 T26431 parataxis Amersham,Sepharose
R7515 T26436 T26435 punct ),Amersham
R7516 T26437 T26405 prep on,mixed
R7517 T26438 T26439 det a,wheel
R7518 T26439 T26437 pobj wheel,on
R7519 T26440 T26439 amod rotating,wheel
R7520 T26441 T26405 prep at,mixed
R7521 T26442 T26443 nummod 4,°C
R7522 T26443 T26441 pobj °C,at
R7523 T26444 T26405 prep for,mixed
R7524 T26445 T26446 nummod 2,hrs
R7525 T26446 T26444 pobj hrs,for
R7526 T26447 T26405 punct .,mixed
R7527 T26449 T26450 compound Protein,concentration
R7528 T26450 T26451 nsubjpass concentration,determined
R7529 T26452 T26451 auxpass was,determined
R7530 T26453 T26451 prep by,determined
R7531 T26454 T26455 det the,system
R7532 T26455 T26453 pobj system,by
R7533 T26456 T26457 compound BCA,assay
R7534 T26457 T26455 compound assay,system
R7535 T26458 T26457 compound protein,assay
R7536 T26459 T26460 punct (,Pierce
R7537 T26460 T26455 parataxis Pierce,system
R7538 T26461 T26460 punct ),Pierce
R7539 T26462 T26451 punct .,determined
R7540 T26464 T26465 det The,beads
R7541 T26465 T26466 nsubjpass beads,washed
R7542 T26467 T26466 auxpass were,washed
R7543 T26468 T26466 advmod then,washed
R7544 T26469 T26470 nummod three,times
R7545 T26470 T26466 npadvmod times,washed
R7546 T26471 T26466 prep with,washed
R7547 T26472 T26473 compound immunoprecipitation,buffer
R7548 T26473 T26471 pobj buffer,with
R7549 T26474 T26466 cc and,washed
R7550 T26475 T26466 conj followed,washed
R7551 T26476 T26475 agent by,followed
R7552 T26477 T26478 nummod three,times
R7553 T26478 T26476 pobj times,by
R7554 T26479 T26478 prep with,times
R7555 T26480 T26481 compound wash,buffer
R7556 T26481 T26479 pobj buffer,with
R7557 T26482 T26483 punct (,HCl
R7558 T26483 T26481 parataxis HCl,buffer
R7559 T26484 T26485 nummod 50,mM
R7560 T26485 T26483 compound mM,HCl
R7561 T26486 T26483 compound Tris,HCl
R7562 T26487 T26483 punct -,HCl
R7563 T26488 T26489 punct [,pH
R7564 T26489 T26483 parataxis pH,HCl
R7565 T26490 T26489 nummod 7.5,pH
R7566 T26491 T26489 punct ],pH
R7567 T26492 T26483 punct ", ",HCl
R7568 T26493 T26494 nummod 150,mM
R7569 T26494 T26495 compound mM,NaCl
R7570 T26495 T26483 appos NaCl,HCl
R7571 T26496 T26483 punct ", ",HCl
R7572 T26497 T26498 nummod 20,mM
R7573 T26498 T26499 compound mM,MgCl2
R7574 T26499 T26483 appos MgCl2,HCl
R7575 T26500 T26483 punct ),HCl
R7576 T26501 T26466 punct .,washed
R7577 T26503 T26504 nsubjpass Proteins,boiled
R7578 T26505 T26504 auxpass were,boiled
R7579 T26506 T26504 advmod then,boiled
R7580 T26507 T26504 prep for,boiled
R7581 T26508 T26509 nummod 5,min
R7582 T26509 T26507 pobj min,for
R7583 T26510 T26504 prep in,boiled
R7584 T26511 T26512 compound SDS,buffer
R7585 T26512 T26510 pobj buffer,in
R7586 T26513 T26512 compound sample,buffer
R7587 T26514 T26515 punct (,SDS
R7588 T26515 T26512 parataxis SDS,buffer
R7589 T26516 T26517 nummod 1,%
R7590 T26517 T26515 compound %,SDS
R7591 T26518 T26515 punct ", ",SDS
R7592 T26519 T26520 nummod 1,mM
R7593 T26520 T26521 compound mM,Tris
R7594 T26521 T26515 appos Tris,SDS
R7595 T26522 T26523 punct [,pH
R7596 T26523 T26521 parataxis pH,Tris
R7597 T26524 T26523 nummod 6.8,pH
R7598 T26525 T26523 punct ],pH
R7599 T26526 T26515 punct ", ",SDS
R7600 T26527 T26528 nummod 40,mM
R7601 T26528 T26529 compound mM,DTT
R7602 T26529 T26515 appos DTT,SDS
R7603 T26530 T26515 punct ", ",SDS
R7604 T26531 T26532 nummod 4,%
R7605 T26532 T26533 compound %,glycerol
R7606 T26533 T26515 appos glycerol,SDS
R7607 T26534 T26515 punct ", ",SDS
R7608 T26535 T26536 nummod 0.01,%
R7609 T26536 T26537 compound %,Y
R7610 T26537 T26515 appos Y,SDS
R7611 T26538 T26537 compound pyronine,Y
R7612 T26539 T26515 punct ),SDS
R7613 T26540 T26504 punct .,boiled
R7614 T26542 T26543 det The,supernatants
R7615 T26543 T26544 nsubjpass supernatants,fractionated
R7616 T26545 T26544 auxpass were,fractionated
R7617 T26546 T26544 prep by,fractionated
R7618 T26547 T26548 nmod sodium,sulfate
R7619 T26548 T26550 nmod sulfate,gel
R7620 T26549 T26548 nmod dodecyl,sulfate
R7621 T26550 T26553 compound gel,electrophoresis
R7622 T26551 T26548 punct -,sulfate
R7623 T26552 T26548 appos polyacrylamide,sulfate
R7624 T26553 T26546 pobj electrophoresis,by
R7625 T26554 T26555 punct (,PAGE
R7626 T26555 T26553 parataxis PAGE,electrophoresis
R7627 T26556 T26555 compound SDS,PAGE
R7628 T26557 T26555 punct -,PAGE
R7629 T26558 T26555 punct ),PAGE
R7630 T26559 T26544 cc and,fractionated
R7631 T26560 T26544 conj transferred,fractionated
R7632 T26561 T26560 prep to,transferred
R7633 T26562 T26563 det the,membrane
R7634 T26563 T26561 pobj membrane,to
R7635 T26564 T26563 compound nitrocellulose,membrane
R7636 T26565 T26566 punct (,Medium
R7637 T26566 T26563 parataxis Medium,membrane
R7638 T26567 T26568 compound Trans,Blot
R7639 T26568 T26566 compound Blot,Medium
R7640 T26569 T26568 punct -,Blot
R7641 T26570 T26566 compound Transfer,Medium
R7642 T26571 T26566 punct ", ",Medium
R7643 T26572 T26573 compound Bio,Rad
R7644 T26573 T26566 appos Rad,Medium
R7645 T26574 T26573 punct -,Rad
R7646 T26575 T26566 punct ),Medium
R7647 T26576 T26544 punct .,fractionated
R7648 T26578 T26579 compound Western,blotting
R7649 T26579 T26580 nsubjpass blotting,performed
R7650 T26581 T26580 auxpass was,performed
R7651 T26582 T26580 prep with,performed
R7652 T26583 T26584 nmod rabbit,antibody
R7653 T26584 T26582 pobj antibody,with
R7654 T26585 T26584 amod polyclonal,antibody
R7655 T26586 T26584 amod anti-HA,antibody
R7656 T26587 T26588 punct (,Cruz
R7657 T26588 T26584 parataxis Cruz,antibody
R7658 T26589 T26588 compound Santa,Cruz
R7659 T26590 T26588 punct ", ",Cruz
R7660 T26591 T26592 punct #,sc
R7661 T26592 T26588 appos sc,Cruz
R7662 T26593 T26592 punct -,sc
R7663 T26594 T26592 nummod 805,sc
R7664 T26595 T26588 punct ),Cruz
R7665 T26596 T26580 punct .,performed
R7666 T26598 T26599 nsubjpass Signals,detected
R7667 T26600 T26599 auxpass were,detected
R7668 T26601 T26599 prep with,detected
R7669 T26602 T26603 compound horseradish,peroxidase
R7670 T26603 T26604 npadvmod peroxidase,conjugated
R7671 T26604 T26606 amod conjugated,IgG
R7672 T26605 T26604 punct -,conjugated
R7673 T26606 T26601 pobj IgG,with
R7674 T26607 T26606 nmod goat,IgG
R7675 T26608 T26606 amod anti-rabbit,IgG
R7676 T26609 T26606 cc and,IgG
R7677 T26610 T26611 nmod ECL,System
R7678 T26611 T26606 conj System,IgG
R7679 T26612 T26610 cc plus,ECL
R7680 T26613 T26614 compound Western,Blotting
R7681 T26614 T26610 conj Blotting,ECL
R7682 T26615 T26611 compound Detection,System
R7683 T26616 T26617 punct (,Amersham
R7684 T26617 T26611 parataxis Amersham,System
R7685 T26618 T26617 punct ),Amersham
R7686 T26619 T26599 punct .,detected