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PMC:1435744 / 43325-44312 JSONTXT

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craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T25085 15-20 NCBITaxon_EXT:yeast denotes yeast
T25086 65-69 PR_EXT:P04386 denotes GAL4
T25087 145-151 SO_EXT:sequence_cloning_process denotes cloned
T25088 168-172 PR_EXT:000014441 denotes mr-s
T25089 189-195 SO_EXT:0000440 denotes vector
T25090 231-236 NCBITaxon:10088 denotes mouse
T25091 243-250 UBERON:0000966 denotes retinal
T25092 251-256 SO_EXT:cDNA denotes cDNAs
T25093 271-277 SO_EXT:0000440 denotes vector
T25094 279-292 GO_EXT:transfectant_or_tranformant denotes Transformants
T25095 308-314 GO_EXT:biological_growth_entity_or_process denotes growth
T25096 404-411 SO_EXT:0000155 denotes Plasmid
T25097 412-415 CHEBI_SO_EXT:DNA denotes DNA
T25098 434-439 NCBITaxon_EXT:yeast denotes yeast
T25099 450-455 NCBITaxon_EXT:yeast denotes Yeast
T25100 456-463 SO_EXT:0000155 denotes Plasmid
T25101 514-521 CHEBI_PR_EXT:protein denotes protein
T25102 568-573 GO:0040007 denotes grown
T25103 623-630 CHEBI_SO_EXT:leucine denotes leucine
T25104 632-642 CHEBI_SO_EXT:tryptophan denotes tryptophan
T25105 658-663 CHEBI:75055 denotes X-gal
T25106 681-686 CHEBI:75055 denotes X-gal
T25107 696-702 SO_EXT:sequence_cloned_entity denotes clones
T25108 759-763 PR_EXT:000014441 denotes mr-s
T25109 795-805 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T25110 807-818 CHEBI_SO_EXT:amino_acid denotes amino acids
T25111 830-840 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminus
T25112 842-853 CHEBI_SO_EXT:amino_acid denotes amino acids
T25113 866-870 PR_EXT:000014441 denotes mr-s
T25114 894-901 SO_EXT:0000440 denotes vectors
T25115 981-986 CHEBI:75055 denotes X-gal

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T25068 65-69 PR:P04386 denotes GAL4
T25069 168-172 PR:000014441 denotes mr-s
T25070 189-195 SO:0000440 denotes vector
T25071 231-236 NCBITaxon:10088 denotes mouse
T25072 243-250 UBERON:0000966 denotes retinal
T25073 271-277 SO:0000440 denotes vector
T25074 404-411 SO:0000155 denotes Plasmid
T25075 456-463 SO:0000155 denotes Plasmid
T25076 568-573 GO:0040007 denotes grown
T25077 658-663 CHEBI:75055 denotes X-gal
T25078 681-686 CHEBI:75055 denotes X-gal
T25079 759-763 PR:000014441 denotes mr-s
T25080 866-870 PR:000014441 denotes mr-s
T25081 894-901 SO:0000440 denotes vectors
T25082 981-986 CHEBI:75055 denotes X-gal

craft-sa-dev

Id Subject Object Predicate Lexical cue
T25125 0-2 PRP denotes We
T25126 3-10 VBD denotes carried
T25127 11-14 RP denotes out
T25128 15-20 NN denotes yeast
T25129 32-43 NNS denotes experiments
T25130 21-24 CD denotes two
T25131 25-31 NN denotes hybrid
T25132 24-25 HYPH denotes -
T25133 44-49 VBG denotes using
T25134 50-53 DT denotes the
T25135 81-87 NN denotes system
T25136 54-64 NNP denotes MATCHMAKER
T25137 65-69 NN denotes GAL4
T25138 70-73 CD denotes two
T25139 74-80 NN denotes hybrid
T25140 73-74 HYPH denotes -
T25141 88-89 CD denotes 3
T25142 90-91 -LRB- denotes (
T25143 94-104 NNP denotes Bioscience
T25144 91-93 NNP denotes BD
T25145 104-105 -RRB- denotes )
T25146 106-108 IN denotes as
T25147 109-120 VBN denotes recommended
T25148 121-123 IN denotes by
T25149 124-127 DT denotes the
T25150 128-140 NN denotes manufacturer
T25151 140-141 . denotes .
T25152 141-278 sentence denotes We cloned the full-length mr-s into the pGBKT7 vector and used it to screen a library of mouse P0-P3 retinal cDNAs in the pGADT7 vector.
T25153 142-144 PRP denotes We
T25154 145-151 VBD denotes cloned
T25155 152-155 DT denotes the
T25156 171-172 NN denotes s
T25157 156-160 JJ denotes full
T25158 161-167 NN denotes length
T25159 160-161 HYPH denotes -
T25160 168-170 NN denotes mr
T25161 170-171 HYPH denotes -
T25162 173-177 IN denotes into
T25163 178-181 DT denotes the
T25164 189-195 NN denotes vector
T25165 182-188 NN denotes pGBKT7
T25166 196-199 CC denotes and
T25167 200-204 VBD denotes used
T25168 205-207 PRP denotes it
T25169 208-210 TO denotes to
T25170 211-217 VB denotes screen
T25171 218-219 DT denotes a
T25172 220-227 NN denotes library
T25173 228-230 IN denotes of
T25174 231-236 NN denotes mouse
T25175 251-256 NNS denotes cDNAs
T25176 237-239 NN denotes P0
T25177 239-240 SYM denotes -
T25178 240-242 NN denotes P3
T25179 243-250 JJ denotes retinal
T25180 257-259 IN denotes in
T25181 260-263 DT denotes the
T25182 271-277 NN denotes vector
T25183 264-270 NN denotes pGADT7
T25184 277-278 . denotes .
T25185 278-403 sentence denotes Transformants that conferred growth were picked, isolated and re-introduced with the bait into AH109 to confirm interaction.
T25186 279-292 NNS denotes Transformants
T25187 320-326 VBN denotes picked
T25188 293-297 WDT denotes that
T25189 298-307 VBD denotes conferred
T25190 308-314 NN denotes growth
T25191 315-319 VBD denotes were
T25192 326-328 , denotes ,
T25193 328-336 VBN denotes isolated
T25194 337-340 CC denotes and
T25195 341-354 VBN denotes re-introduced
T25196 355-359 IN denotes with
T25197 360-363 DT denotes the
T25198 364-368 NN denotes bait
T25199 369-373 IN denotes into
T25200 374-379 NN denotes AH109
T25201 380-382 TO denotes to
T25202 383-390 VB denotes confirm
T25203 391-402 NN denotes interaction
T25204 402-403 . denotes .
T25205 403-489 sentence denotes Plasmid DNA was isolated from yeast using RPM Yeast Plasmid Isolation Kit (Qbiogene).
T25206 404-411 NN denotes Plasmid
T25207 412-415 NN denotes DNA
T25208 420-428 VBN denotes isolated
T25209 416-419 VBD denotes was
T25210 429-433 IN denotes from
T25211 434-439 NN denotes yeast
T25212 440-445 VBG denotes using
T25213 446-449 NN denotes RPM
T25214 474-477 NN denotes Kit
T25215 450-455 NN denotes Yeast
T25216 456-463 NN denotes Plasmid
T25217 464-473 NN denotes Isolation
T25218 478-479 -LRB- denotes (
T25219 479-487 NNP denotes Qbiogene
T25220 487-488 -RRB- denotes )
T25221 488-489 . denotes .
T25222 489-664 sentence denotes In order to confirm the protein interactions, single colonies were picked and grown individually in synthetic complete media lacking leucine, tryptophan and containing X-gal.
T25223 490-492 IN denotes In
T25224 557-563 VBN denotes picked
T25225 493-498 NN denotes order
T25226 499-501 TO denotes to
T25227 502-509 VB denotes confirm
T25228 510-513 DT denotes the
T25229 522-534 NNS denotes interactions
T25230 514-521 NN denotes protein
T25231 534-536 , denotes ,
T25232 536-542 JJ denotes single
T25233 543-551 NNS denotes colonies
T25234 552-556 VBD denotes were
T25235 564-567 CC denotes and
T25236 568-573 VBN denotes grown
T25237 574-586 RB denotes individually
T25238 587-589 IN denotes in
T25239 590-599 JJ denotes synthetic
T25240 609-614 NNS denotes media
T25241 600-608 JJ denotes complete
T25242 615-622 VBG denotes lacking
T25243 623-630 NN denotes leucine
T25244 632-642 NN denotes tryptophan
T25245 630-632 , denotes ,
T25246 643-646 CC denotes and
T25247 647-657 VBG denotes containing
T25248 658-659 NN denotes X
T25249 660-663 NN denotes gal
T25250 659-660 HYPH denotes -
T25251 663-664 . denotes .
T25252 664-730 sentence denotes After 4–5 days, X-gal positive clones were selected and analyzed.
T25253 665-670 IN denotes After
T25254 708-716 VBN denotes selected
T25255 671-672 CD denotes 4
T25256 673-674 CD denotes 5
T25257 672-673 SYM denotes –
T25258 675-679 NNS denotes days
T25259 679-681 , denotes ,
T25260 681-682 NN denotes X
T25261 683-686 NN denotes gal
T25262 682-683 NN denotes -
T25263 687-695 JJ denotes positive
T25264 696-702 NNS denotes clones
T25265 703-707 VBD denotes were
T25266 717-720 CC denotes and
T25267 721-729 VBN denotes analyzed
T25268 729-730 . denotes .
T25269 730-902 sentence denotes To test self-interaction of mr-s, we subcloned the full-length, N-terminus (amino acids 1–400) and C-terminus (amino acids 391–542) of mr-s into pGBKT7 and pGADT7 vectors.
T25270 731-733 TO denotes To
T25271 734-738 VB denotes test
T25272 768-777 VBD denotes subcloned
T25273 739-743 NN denotes self
T25274 744-755 NN denotes interaction
T25275 743-744 HYPH denotes -
T25276 756-758 IN denotes of
T25277 759-761 NN denotes mr
T25278 762-763 NN denotes s
T25279 761-762 HYPH denotes -
T25280 763-765 , denotes ,
T25281 765-767 PRP denotes we
T25282 778-781 DT denotes the
T25283 787-793 NN denotes length
T25284 782-786 JJ denotes full
T25285 786-787 HYPH denotes -
T25286 793-795 , denotes ,
T25287 795-796 NN denotes N
T25288 797-805 NN denotes terminus
T25289 796-797 HYPH denotes -
T25290 806-807 -LRB- denotes (
T25291 819-820 CD denotes 1
T25292 807-812 NN denotes amino
T25293 813-818 NNS denotes acids
T25294 820-821 SYM denotes –
T25295 821-824 CD denotes 400
T25296 824-825 -RRB- denotes )
T25297 826-829 CC denotes and
T25298 830-831 NN denotes C
T25299 832-840 NN denotes terminus
T25300 831-832 HYPH denotes -
T25301 841-842 -LRB- denotes (
T25302 854-857 CD denotes 391
T25303 842-847 NN denotes amino
T25304 848-853 NNS denotes acids
T25305 857-858 SYM denotes –
T25306 858-861 CD denotes 542
T25307 861-862 -RRB- denotes )
T25308 863-865 IN denotes of
T25309 866-868 NN denotes mr
T25310 869-870 NN denotes s
T25311 868-869 HYPH denotes -
T25312 871-875 IN denotes into
T25313 876-882 NN denotes pGBKT7
T25314 894-901 NNS denotes vectors
T25315 883-886 CC denotes and
T25316 887-893 NN denotes pGADT7
T25317 901-902 . denotes .
T25318 902-987 sentence denotes We assessed interactions by scoring blue color on plates of medium containing X-gal.
T25319 903-905 PRP denotes We
T25320 906-914 VBD denotes assessed
T25321 915-927 NNS denotes interactions
T25322 928-930 IN denotes by
T25323 931-938 VBG denotes scoring
T25324 939-943 JJ denotes blue
T25325 944-949 NN denotes color
T25326 950-952 IN denotes on
T25327 953-959 NNS denotes plates
T25328 960-962 IN denotes of
T25329 963-969 NN denotes medium
T25330 970-980 VBG denotes containing
T25331 981-982 NN denotes X
T25332 983-986 NN denotes gal
T25333 982-983 HYPH denotes -
T25334 986-987 . denotes .
R7095 T25125 T25126 nsubj We,carried
R7096 T25127 T25126 prt out,carried
R7097 T25128 T25129 nmod yeast,experiments
R7098 T25129 T25126 dobj experiments,carried
R7099 T25130 T25131 nummod two,hybrid
R7100 T25131 T25129 compound hybrid,experiments
R7101 T25132 T25131 punct -,hybrid
R7102 T25133 T25126 advcl using,carried
R7103 T25134 T25135 det the,system
R7104 T25135 T25133 dobj system,using
R7105 T25136 T25135 nmod MATCHMAKER,system
R7106 T25137 T25135 nmod GAL4,system
R7107 T25138 T25139 nummod two,hybrid
R7108 T25139 T25135 compound hybrid,system
R7109 T25140 T25139 punct -,hybrid
R7110 T25141 T25135 nummod 3,system
R7111 T25142 T25143 punct (,Bioscience
R7112 T25143 T25135 parataxis Bioscience,system
R7113 T25144 T25143 compound BD,Bioscience
R7114 T25145 T25143 punct ),Bioscience
R7115 T25146 T25147 mark as,recommended
R7116 T25147 T25126 advcl recommended,carried
R7117 T25148 T25147 agent by,recommended
R7118 T25149 T25150 det the,manufacturer
R7119 T25150 T25148 pobj manufacturer,by
R7120 T25151 T25126 punct .,carried
R7121 T25153 T25154 nsubj We,cloned
R7122 T25155 T25156 det the,s
R7123 T25156 T25154 dobj s,cloned
R7124 T25157 T25158 amod full,length
R7125 T25158 T25156 compound length,s
R7126 T25159 T25158 punct -,length
R7127 T25160 T25156 compound mr,s
R7128 T25161 T25156 punct -,s
R7129 T25162 T25154 prep into,cloned
R7130 T25163 T25164 det the,vector
R7131 T25164 T25162 pobj vector,into
R7132 T25165 T25164 compound pGBKT7,vector
R7133 T25166 T25154 cc and,cloned
R7134 T25167 T25154 conj used,cloned
R7135 T25168 T25167 dobj it,used
R7136 T25169 T25170 aux to,screen
R7137 T25170 T25167 advcl screen,used
R7138 T25171 T25172 det a,library
R7139 T25172 T25170 dobj library,screen
R7140 T25173 T25172 prep of,library
R7141 T25174 T25175 nmod mouse,cDNAs
R7142 T25175 T25173 pobj cDNAs,of
R7143 T25176 T25175 nmod P0,cDNAs
R7144 T25177 T25178 punct -,P3
R7145 T25178 T25176 prep P3,P0
R7146 T25179 T25175 amod retinal,cDNAs
R7147 T25180 T25175 prep in,cDNAs
R7148 T25181 T25182 det the,vector
R7149 T25182 T25180 pobj vector,in
R7150 T25183 T25182 compound pGADT7,vector
R7151 T25184 T25154 punct .,cloned
R7152 T25186 T25187 nsubj Transformants,picked
R7153 T25188 T25189 dep that,conferred
R7154 T25189 T25186 relcl conferred,Transformants
R7155 T25190 T25189 dobj growth,conferred
R7156 T25191 T25187 aux were,picked
R7157 T25192 T25187 punct ", ",picked
R7158 T25193 T25187 conj isolated,picked
R7159 T25194 T25193 cc and,isolated
R7160 T25195 T25193 conj re-introduced,isolated
R7161 T25196 T25195 prep with,re-introduced
R7162 T25197 T25198 det the,bait
R7163 T25198 T25196 pobj bait,with
R7164 T25199 T25195 prep into,re-introduced
R7165 T25200 T25199 pobj AH109,into
R7166 T25201 T25202 aux to,confirm
R7167 T25202 T25187 advcl confirm,picked
R7168 T25203 T25202 dobj interaction,confirm
R7169 T25204 T25187 punct .,picked
R7170 T25206 T25207 compound Plasmid,DNA
R7171 T25207 T25208 nsubjpass DNA,isolated
R7172 T25209 T25208 auxpass was,isolated
R7173 T25210 T25208 prep from,isolated
R7174 T25211 T25210 pobj yeast,from
R7175 T25212 T25208 advcl using,isolated
R7176 T25213 T25214 compound RPM,Kit
R7177 T25214 T25212 dobj Kit,using
R7178 T25215 T25216 compound Yeast,Plasmid
R7179 T25216 T25217 compound Plasmid,Isolation
R7180 T25217 T25214 compound Isolation,Kit
R7181 T25218 T25219 punct (,Qbiogene
R7182 T25219 T25214 parataxis Qbiogene,Kit
R7183 T25220 T25219 punct ),Qbiogene
R7184 T25221 T25208 punct .,isolated
R7185 T25223 T25224 prep In,picked
R7186 T25225 T25223 pobj order,In
R7187 T25226 T25227 aux to,confirm
R7188 T25227 T25225 acl confirm,order
R7189 T25228 T25229 det the,interactions
R7190 T25229 T25227 dobj interactions,confirm
R7191 T25230 T25229 compound protein,interactions
R7192 T25231 T25224 punct ", ",picked
R7193 T25232 T25233 amod single,colonies
R7194 T25233 T25224 nsubjpass colonies,picked
R7195 T25234 T25224 auxpass were,picked
R7196 T25235 T25224 cc and,picked
R7197 T25236 T25224 conj grown,picked
R7198 T25237 T25236 advmod individually,grown
R7199 T25238 T25236 prep in,grown
R7200 T25239 T25240 amod synthetic,media
R7201 T25240 T25238 pobj media,in
R7202 T25241 T25240 amod complete,media
R7203 T25242 T25240 acl lacking,media
R7204 T25243 T25244 nmod leucine,tryptophan
R7205 T25244 T25242 dobj tryptophan,lacking
R7206 T25245 T25244 punct ", ",tryptophan
R7207 T25246 T25242 cc and,lacking
R7208 T25247 T25242 conj containing,lacking
R7209 T25248 T25249 compound X,gal
R7210 T25249 T25247 dobj gal,containing
R7211 T25250 T25249 punct -,gal
R7212 T25251 T25224 punct .,picked
R7213 T25253 T25254 prep After,selected
R7214 T25255 T25256 quantmod 4,5
R7215 T25256 T25258 nummod 5,days
R7216 T25257 T25256 punct –,5
R7217 T25258 T25253 pobj days,After
R7218 T25259 T25254 punct ", ",selected
R7219 T25260 T25261 compound X,gal
R7220 T25261 T25263 npadvmod gal,positive
R7221 T25262 T25261 compound -,gal
R7222 T25263 T25264 amod positive,clones
R7223 T25264 T25254 nsubjpass clones,selected
R7224 T25265 T25254 auxpass were,selected
R7225 T25266 T25254 cc and,selected
R7226 T25267 T25254 conj analyzed,selected
R7227 T25268 T25254 punct .,selected
R7228 T25270 T25271 aux To,test
R7229 T25271 T25272 advcl test,subcloned
R7230 T25273 T25274 compound self,interaction
R7231 T25274 T25271 dobj interaction,test
R7232 T25275 T25274 punct -,interaction
R7233 T25276 T25274 prep of,interaction
R7234 T25277 T25278 compound mr,s
R7235 T25278 T25276 pobj s,of
R7236 T25279 T25278 punct -,s
R7237 T25280 T25272 punct ", ",subcloned
R7238 T25281 T25272 nsubj we,subcloned
R7239 T25282 T25283 det the,length
R7240 T25283 T25272 dobj length,subcloned
R7241 T25284 T25283 amod full,length
R7242 T25285 T25283 punct -,length
R7243 T25286 T25283 punct ", ",length
R7244 T25287 T25288 compound N,terminus
R7245 T25288 T25283 conj terminus,length
R7246 T25289 T25288 punct -,terminus
R7247 T25290 T25291 punct (,1
R7248 T25291 T25288 parataxis 1,terminus
R7249 T25292 T25291 nmod amino,1
R7250 T25293 T25291 nmod acids,1
R7251 T25294 T25295 punct –,400
R7252 T25295 T25291 prep 400,1
R7253 T25296 T25291 punct ),1
R7254 T25297 T25288 cc and,terminus
R7255 T25298 T25299 compound C,terminus
R7256 T25299 T25288 conj terminus,terminus
R7257 T25300 T25299 punct -,terminus
R7258 T25301 T25302 punct (,391
R7259 T25302 T25299 parataxis 391,terminus
R7260 T25303 T25302 nmod amino,391
R7261 T25304 T25302 nmod acids,391
R7262 T25305 T25306 punct –,542
R7263 T25306 T25302 prep 542,391
R7264 T25307 T25302 punct ),391
R7265 T25308 T25283 prep of,length
R7266 T25309 T25310 compound mr,s
R7267 T25310 T25308 pobj s,of
R7268 T25311 T25310 punct -,s
R7269 T25312 T25272 prep into,subcloned
R7270 T25313 T25314 nmod pGBKT7,vectors
R7271 T25314 T25312 pobj vectors,into
R7272 T25315 T25313 cc and,pGBKT7
R7273 T25316 T25313 conj pGADT7,pGBKT7
R7274 T25317 T25272 punct .,subcloned
R7275 T25319 T25320 nsubj We,assessed
R7276 T25321 T25320 dobj interactions,assessed
R7277 T25322 T25320 prep by,assessed
R7278 T25323 T25322 pcomp scoring,by
R7279 T25324 T25325 amod blue,color
R7280 T25325 T25323 dobj color,scoring
R7281 T25326 T25323 prep on,scoring
R7282 T25327 T25326 pobj plates,on
R7283 T25328 T25327 prep of,plates
R7284 T25329 T25328 pobj medium,of
R7285 T25330 T25327 acl containing,plates
R7286 T25331 T25332 compound X,gal
R7287 T25332 T25330 dobj gal,containing
R7288 T25333 T25332 punct -,gal
R7289 T25334 T25320 punct .,assessed