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PMC:1435744 / 43283-44312 JSONTXT

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craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T25083 0-5 NCBITaxon_EXT:yeast denotes Yeast
T25084 31-35 PR_EXT:P04386 denotes GAL4
T25085 57-62 NCBITaxon_EXT:yeast denotes yeast
T25086 107-111 PR_EXT:P04386 denotes GAL4
T25087 187-193 SO_EXT:sequence_cloning_process denotes cloned
T25088 210-214 PR_EXT:000014441 denotes mr-s
T25089 231-237 SO_EXT:0000440 denotes vector
T25090 273-278 NCBITaxon:10088 denotes mouse
T25091 285-292 UBERON:0000966 denotes retinal
T25092 293-298 SO_EXT:cDNA denotes cDNAs
T25093 313-319 SO_EXT:0000440 denotes vector
T25094 321-334 GO_EXT:transfectant_or_tranformant denotes Transformants
T25095 350-356 GO_EXT:biological_growth_entity_or_process denotes growth
T25096 446-453 SO_EXT:0000155 denotes Plasmid
T25097 454-457 CHEBI_SO_EXT:DNA denotes DNA
T25098 476-481 NCBITaxon_EXT:yeast denotes yeast
T25099 492-497 NCBITaxon_EXT:yeast denotes Yeast
T25100 498-505 SO_EXT:0000155 denotes Plasmid
T25101 556-563 CHEBI_PR_EXT:protein denotes protein
T25102 610-615 GO:0040007 denotes grown
T25103 665-672 CHEBI_SO_EXT:leucine denotes leucine
T25104 674-684 CHEBI_SO_EXT:tryptophan denotes tryptophan
T25105 700-705 CHEBI:75055 denotes X-gal
T25106 723-728 CHEBI:75055 denotes X-gal
T25107 738-744 SO_EXT:sequence_cloned_entity denotes clones
T25108 801-805 PR_EXT:000014441 denotes mr-s
T25109 837-847 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T25110 849-860 CHEBI_SO_EXT:amino_acid denotes amino acids
T25111 872-882 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminus
T25112 884-895 CHEBI_SO_EXT:amino_acid denotes amino acids
T25113 908-912 PR_EXT:000014441 denotes mr-s
T25114 936-943 SO_EXT:0000440 denotes vectors
T25115 1023-1028 CHEBI:75055 denotes X-gal

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T25067 31-35 PR:P04386 denotes GAL4
T25068 107-111 PR:P04386 denotes GAL4
T25069 210-214 PR:000014441 denotes mr-s
T25070 231-237 SO:0000440 denotes vector
T25071 273-278 NCBITaxon:10088 denotes mouse
T25072 285-292 UBERON:0000966 denotes retinal
T25073 313-319 SO:0000440 denotes vector
T25074 446-453 SO:0000155 denotes Plasmid
T25075 498-505 SO:0000155 denotes Plasmid
T25076 610-615 GO:0040007 denotes grown
T25077 700-705 CHEBI:75055 denotes X-gal
T25078 723-728 CHEBI:75055 denotes X-gal
T25079 801-805 PR:000014441 denotes mr-s
T25080 908-912 PR:000014441 denotes mr-s
T25081 936-943 SO:0000440 denotes vectors
T25082 1023-1028 CHEBI:75055 denotes X-gal

craft-sa-dev

Id Subject Object Predicate Lexical cue
T25116 0-5 NN denotes Yeast
T25117 17-26 NN denotes screening
T25118 6-9 CD denotes two
T25119 10-16 NN denotes hybrid
T25120 9-10 HYPH denotes -
T25121 27-30 CC denotes and
T25122 31-35 NN denotes GAL4
T25123 36-41 NN denotes assay
T25124 41-183 sentence denotes We carried out yeast two-hybrid experiments using the MATCHMAKER GAL4 two-hybrid system 3 (BD Bioscience) as recommended by the manufacturer.
T25125 42-44 PRP denotes We
T25126 45-52 VBD denotes carried
T25127 53-56 RP denotes out
T25128 57-62 NN denotes yeast
T25129 74-85 NNS denotes experiments
T25130 63-66 CD denotes two
T25131 67-73 NN denotes hybrid
T25132 66-67 HYPH denotes -
T25133 86-91 VBG denotes using
T25134 92-95 DT denotes the
T25135 123-129 NN denotes system
T25136 96-106 NNP denotes MATCHMAKER
T25137 107-111 NN denotes GAL4
T25138 112-115 CD denotes two
T25139 116-122 NN denotes hybrid
T25140 115-116 HYPH denotes -
T25141 130-131 CD denotes 3
T25142 132-133 -LRB- denotes (
T25143 136-146 NNP denotes Bioscience
T25144 133-135 NNP denotes BD
T25145 146-147 -RRB- denotes )
T25146 148-150 IN denotes as
T25147 151-162 VBN denotes recommended
T25148 163-165 IN denotes by
T25149 166-169 DT denotes the
T25150 170-182 NN denotes manufacturer
T25151 182-183 . denotes .
T25152 183-320 sentence denotes We cloned the full-length mr-s into the pGBKT7 vector and used it to screen a library of mouse P0-P3 retinal cDNAs in the pGADT7 vector.
T25153 184-186 PRP denotes We
T25154 187-193 VBD denotes cloned
T25155 194-197 DT denotes the
T25156 213-214 NN denotes s
T25157 198-202 JJ denotes full
T25158 203-209 NN denotes length
T25159 202-203 HYPH denotes -
T25160 210-212 NN denotes mr
T25161 212-213 HYPH denotes -
T25162 215-219 IN denotes into
T25163 220-223 DT denotes the
T25164 231-237 NN denotes vector
T25165 224-230 NN denotes pGBKT7
T25166 238-241 CC denotes and
T25167 242-246 VBD denotes used
T25168 247-249 PRP denotes it
T25169 250-252 TO denotes to
T25170 253-259 VB denotes screen
T25171 260-261 DT denotes a
T25172 262-269 NN denotes library
T25173 270-272 IN denotes of
T25174 273-278 NN denotes mouse
T25175 293-298 NNS denotes cDNAs
T25176 279-281 NN denotes P0
T25177 281-282 SYM denotes -
T25178 282-284 NN denotes P3
T25179 285-292 JJ denotes retinal
T25180 299-301 IN denotes in
T25181 302-305 DT denotes the
T25182 313-319 NN denotes vector
T25183 306-312 NN denotes pGADT7
T25184 319-320 . denotes .
T25185 320-445 sentence denotes Transformants that conferred growth were picked, isolated and re-introduced with the bait into AH109 to confirm interaction.
T25186 321-334 NNS denotes Transformants
T25187 362-368 VBN denotes picked
T25188 335-339 WDT denotes that
T25189 340-349 VBD denotes conferred
T25190 350-356 NN denotes growth
T25191 357-361 VBD denotes were
T25192 368-370 , denotes ,
T25193 370-378 VBN denotes isolated
T25194 379-382 CC denotes and
T25195 383-396 VBN denotes re-introduced
T25196 397-401 IN denotes with
T25197 402-405 DT denotes the
T25198 406-410 NN denotes bait
T25199 411-415 IN denotes into
T25200 416-421 NN denotes AH109
T25201 422-424 TO denotes to
T25202 425-432 VB denotes confirm
T25203 433-444 NN denotes interaction
T25204 444-445 . denotes .
T25205 445-531 sentence denotes Plasmid DNA was isolated from yeast using RPM Yeast Plasmid Isolation Kit (Qbiogene).
T25206 446-453 NN denotes Plasmid
T25207 454-457 NN denotes DNA
T25208 462-470 VBN denotes isolated
T25209 458-461 VBD denotes was
T25210 471-475 IN denotes from
T25211 476-481 NN denotes yeast
T25212 482-487 VBG denotes using
T25213 488-491 NN denotes RPM
T25214 516-519 NN denotes Kit
T25215 492-497 NN denotes Yeast
T25216 498-505 NN denotes Plasmid
T25217 506-515 NN denotes Isolation
T25218 520-521 -LRB- denotes (
T25219 521-529 NNP denotes Qbiogene
T25220 529-530 -RRB- denotes )
T25221 530-531 . denotes .
T25222 531-706 sentence denotes In order to confirm the protein interactions, single colonies were picked and grown individually in synthetic complete media lacking leucine, tryptophan and containing X-gal.
T25223 532-534 IN denotes In
T25224 599-605 VBN denotes picked
T25225 535-540 NN denotes order
T25226 541-543 TO denotes to
T25227 544-551 VB denotes confirm
T25228 552-555 DT denotes the
T25229 564-576 NNS denotes interactions
T25230 556-563 NN denotes protein
T25231 576-578 , denotes ,
T25232 578-584 JJ denotes single
T25233 585-593 NNS denotes colonies
T25234 594-598 VBD denotes were
T25235 606-609 CC denotes and
T25236 610-615 VBN denotes grown
T25237 616-628 RB denotes individually
T25238 629-631 IN denotes in
T25239 632-641 JJ denotes synthetic
T25240 651-656 NNS denotes media
T25241 642-650 JJ denotes complete
T25242 657-664 VBG denotes lacking
T25243 665-672 NN denotes leucine
T25244 674-684 NN denotes tryptophan
T25245 672-674 , denotes ,
T25246 685-688 CC denotes and
T25247 689-699 VBG denotes containing
T25248 700-701 NN denotes X
T25249 702-705 NN denotes gal
T25250 701-702 HYPH denotes -
T25251 705-706 . denotes .
T25252 706-772 sentence denotes After 4–5 days, X-gal positive clones were selected and analyzed.
T25253 707-712 IN denotes After
T25254 750-758 VBN denotes selected
T25255 713-714 CD denotes 4
T25256 715-716 CD denotes 5
T25257 714-715 SYM denotes –
T25258 717-721 NNS denotes days
T25259 721-723 , denotes ,
T25260 723-724 NN denotes X
T25261 725-728 NN denotes gal
T25262 724-725 NN denotes -
T25263 729-737 JJ denotes positive
T25264 738-744 NNS denotes clones
T25265 745-749 VBD denotes were
T25266 759-762 CC denotes and
T25267 763-771 VBN denotes analyzed
T25268 771-772 . denotes .
T25269 772-944 sentence denotes To test self-interaction of mr-s, we subcloned the full-length, N-terminus (amino acids 1–400) and C-terminus (amino acids 391–542) of mr-s into pGBKT7 and pGADT7 vectors.
T25270 773-775 TO denotes To
T25271 776-780 VB denotes test
T25272 810-819 VBD denotes subcloned
T25273 781-785 NN denotes self
T25274 786-797 NN denotes interaction
T25275 785-786 HYPH denotes -
T25276 798-800 IN denotes of
T25277 801-803 NN denotes mr
T25278 804-805 NN denotes s
T25279 803-804 HYPH denotes -
T25280 805-807 , denotes ,
T25281 807-809 PRP denotes we
T25282 820-823 DT denotes the
T25283 829-835 NN denotes length
T25284 824-828 JJ denotes full
T25285 828-829 HYPH denotes -
T25286 835-837 , denotes ,
T25287 837-838 NN denotes N
T25288 839-847 NN denotes terminus
T25289 838-839 HYPH denotes -
T25290 848-849 -LRB- denotes (
T25291 861-862 CD denotes 1
T25292 849-854 NN denotes amino
T25293 855-860 NNS denotes acids
T25294 862-863 SYM denotes –
T25295 863-866 CD denotes 400
T25296 866-867 -RRB- denotes )
T25297 868-871 CC denotes and
T25298 872-873 NN denotes C
T25299 874-882 NN denotes terminus
T25300 873-874 HYPH denotes -
T25301 883-884 -LRB- denotes (
T25302 896-899 CD denotes 391
T25303 884-889 NN denotes amino
T25304 890-895 NNS denotes acids
T25305 899-900 SYM denotes –
T25306 900-903 CD denotes 542
T25307 903-904 -RRB- denotes )
T25308 905-907 IN denotes of
T25309 908-910 NN denotes mr
T25310 911-912 NN denotes s
T25311 910-911 HYPH denotes -
T25312 913-917 IN denotes into
T25313 918-924 NN denotes pGBKT7
T25314 936-943 NNS denotes vectors
T25315 925-928 CC denotes and
T25316 929-935 NN denotes pGADT7
T25317 943-944 . denotes .
T25318 944-1029 sentence denotes We assessed interactions by scoring blue color on plates of medium containing X-gal.
T25319 945-947 PRP denotes We
T25320 948-956 VBD denotes assessed
T25321 957-969 NNS denotes interactions
T25322 970-972 IN denotes by
T25323 973-980 VBG denotes scoring
T25324 981-985 JJ denotes blue
T25325 986-991 NN denotes color
T25326 992-994 IN denotes on
T25327 995-1001 NNS denotes plates
T25328 1002-1004 IN denotes of
T25329 1005-1011 NN denotes medium
T25330 1012-1022 VBG denotes containing
T25331 1023-1024 NN denotes X
T25332 1025-1028 NN denotes gal
T25333 1024-1025 HYPH denotes -
T25334 1028-1029 . denotes .
R7088 T25116 T25117 nmod Yeast,screening
R7089 T25118 T25119 nummod two,hybrid
R7090 T25119 T25117 compound hybrid,screening
R7091 T25120 T25119 punct -,hybrid
R7092 T25121 T25117 cc and,screening
R7093 T25122 T25123 compound GAL4,assay
R7094 T25123 T25117 conj assay,screening
R7095 T25125 T25126 nsubj We,carried
R7096 T25127 T25126 prt out,carried
R7097 T25128 T25129 nmod yeast,experiments
R7098 T25129 T25126 dobj experiments,carried
R7099 T25130 T25131 nummod two,hybrid
R7100 T25131 T25129 compound hybrid,experiments
R7101 T25132 T25131 punct -,hybrid
R7102 T25133 T25126 advcl using,carried
R7103 T25134 T25135 det the,system
R7104 T25135 T25133 dobj system,using
R7105 T25136 T25135 nmod MATCHMAKER,system
R7106 T25137 T25135 nmod GAL4,system
R7107 T25138 T25139 nummod two,hybrid
R7108 T25139 T25135 compound hybrid,system
R7109 T25140 T25139 punct -,hybrid
R7110 T25141 T25135 nummod 3,system
R7111 T25142 T25143 punct (,Bioscience
R7112 T25143 T25135 parataxis Bioscience,system
R7113 T25144 T25143 compound BD,Bioscience
R7114 T25145 T25143 punct ),Bioscience
R7115 T25146 T25147 mark as,recommended
R7116 T25147 T25126 advcl recommended,carried
R7117 T25148 T25147 agent by,recommended
R7118 T25149 T25150 det the,manufacturer
R7119 T25150 T25148 pobj manufacturer,by
R7120 T25151 T25126 punct .,carried
R7121 T25153 T25154 nsubj We,cloned
R7122 T25155 T25156 det the,s
R7123 T25156 T25154 dobj s,cloned
R7124 T25157 T25158 amod full,length
R7125 T25158 T25156 compound length,s
R7126 T25159 T25158 punct -,length
R7127 T25160 T25156 compound mr,s
R7128 T25161 T25156 punct -,s
R7129 T25162 T25154 prep into,cloned
R7130 T25163 T25164 det the,vector
R7131 T25164 T25162 pobj vector,into
R7132 T25165 T25164 compound pGBKT7,vector
R7133 T25166 T25154 cc and,cloned
R7134 T25167 T25154 conj used,cloned
R7135 T25168 T25167 dobj it,used
R7136 T25169 T25170 aux to,screen
R7137 T25170 T25167 advcl screen,used
R7138 T25171 T25172 det a,library
R7139 T25172 T25170 dobj library,screen
R7140 T25173 T25172 prep of,library
R7141 T25174 T25175 nmod mouse,cDNAs
R7142 T25175 T25173 pobj cDNAs,of
R7143 T25176 T25175 nmod P0,cDNAs
R7144 T25177 T25178 punct -,P3
R7145 T25178 T25176 prep P3,P0
R7146 T25179 T25175 amod retinal,cDNAs
R7147 T25180 T25175 prep in,cDNAs
R7148 T25181 T25182 det the,vector
R7149 T25182 T25180 pobj vector,in
R7150 T25183 T25182 compound pGADT7,vector
R7151 T25184 T25154 punct .,cloned
R7152 T25186 T25187 nsubj Transformants,picked
R7153 T25188 T25189 dep that,conferred
R7154 T25189 T25186 relcl conferred,Transformants
R7155 T25190 T25189 dobj growth,conferred
R7156 T25191 T25187 aux were,picked
R7157 T25192 T25187 punct ", ",picked
R7158 T25193 T25187 conj isolated,picked
R7159 T25194 T25193 cc and,isolated
R7160 T25195 T25193 conj re-introduced,isolated
R7161 T25196 T25195 prep with,re-introduced
R7162 T25197 T25198 det the,bait
R7163 T25198 T25196 pobj bait,with
R7164 T25199 T25195 prep into,re-introduced
R7165 T25200 T25199 pobj AH109,into
R7166 T25201 T25202 aux to,confirm
R7167 T25202 T25187 advcl confirm,picked
R7168 T25203 T25202 dobj interaction,confirm
R7169 T25204 T25187 punct .,picked
R7170 T25206 T25207 compound Plasmid,DNA
R7171 T25207 T25208 nsubjpass DNA,isolated
R7172 T25209 T25208 auxpass was,isolated
R7173 T25210 T25208 prep from,isolated
R7174 T25211 T25210 pobj yeast,from
R7175 T25212 T25208 advcl using,isolated
R7176 T25213 T25214 compound RPM,Kit
R7177 T25214 T25212 dobj Kit,using
R7178 T25215 T25216 compound Yeast,Plasmid
R7179 T25216 T25217 compound Plasmid,Isolation
R7180 T25217 T25214 compound Isolation,Kit
R7181 T25218 T25219 punct (,Qbiogene
R7182 T25219 T25214 parataxis Qbiogene,Kit
R7183 T25220 T25219 punct ),Qbiogene
R7184 T25221 T25208 punct .,isolated
R7185 T25223 T25224 prep In,picked
R7186 T25225 T25223 pobj order,In
R7187 T25226 T25227 aux to,confirm
R7188 T25227 T25225 acl confirm,order
R7189 T25228 T25229 det the,interactions
R7190 T25229 T25227 dobj interactions,confirm
R7191 T25230 T25229 compound protein,interactions
R7192 T25231 T25224 punct ", ",picked
R7193 T25232 T25233 amod single,colonies
R7194 T25233 T25224 nsubjpass colonies,picked
R7195 T25234 T25224 auxpass were,picked
R7196 T25235 T25224 cc and,picked
R7197 T25236 T25224 conj grown,picked
R7198 T25237 T25236 advmod individually,grown
R7199 T25238 T25236 prep in,grown
R7200 T25239 T25240 amod synthetic,media
R7201 T25240 T25238 pobj media,in
R7202 T25241 T25240 amod complete,media
R7203 T25242 T25240 acl lacking,media
R7204 T25243 T25244 nmod leucine,tryptophan
R7205 T25244 T25242 dobj tryptophan,lacking
R7206 T25245 T25244 punct ", ",tryptophan
R7207 T25246 T25242 cc and,lacking
R7208 T25247 T25242 conj containing,lacking
R7209 T25248 T25249 compound X,gal
R7210 T25249 T25247 dobj gal,containing
R7211 T25250 T25249 punct -,gal
R7212 T25251 T25224 punct .,picked
R7213 T25253 T25254 prep After,selected
R7214 T25255 T25256 quantmod 4,5
R7215 T25256 T25258 nummod 5,days
R7216 T25257 T25256 punct –,5
R7217 T25258 T25253 pobj days,After
R7218 T25259 T25254 punct ", ",selected
R7219 T25260 T25261 compound X,gal
R7220 T25261 T25263 npadvmod gal,positive
R7221 T25262 T25261 compound -,gal
R7222 T25263 T25264 amod positive,clones
R7223 T25264 T25254 nsubjpass clones,selected
R7224 T25265 T25254 auxpass were,selected
R7225 T25266 T25254 cc and,selected
R7226 T25267 T25254 conj analyzed,selected
R7227 T25268 T25254 punct .,selected
R7228 T25270 T25271 aux To,test
R7229 T25271 T25272 advcl test,subcloned
R7230 T25273 T25274 compound self,interaction
R7231 T25274 T25271 dobj interaction,test
R7232 T25275 T25274 punct -,interaction
R7233 T25276 T25274 prep of,interaction
R7234 T25277 T25278 compound mr,s
R7235 T25278 T25276 pobj s,of
R7236 T25279 T25278 punct -,s
R7237 T25280 T25272 punct ", ",subcloned
R7238 T25281 T25272 nsubj we,subcloned
R7239 T25282 T25283 det the,length
R7240 T25283 T25272 dobj length,subcloned
R7241 T25284 T25283 amod full,length
R7242 T25285 T25283 punct -,length
R7243 T25286 T25283 punct ", ",length
R7244 T25287 T25288 compound N,terminus
R7245 T25288 T25283 conj terminus,length
R7246 T25289 T25288 punct -,terminus
R7247 T25290 T25291 punct (,1
R7248 T25291 T25288 parataxis 1,terminus
R7249 T25292 T25291 nmod amino,1
R7250 T25293 T25291 nmod acids,1
R7251 T25294 T25295 punct –,400
R7252 T25295 T25291 prep 400,1
R7253 T25296 T25291 punct ),1
R7254 T25297 T25288 cc and,terminus
R7255 T25298 T25299 compound C,terminus
R7256 T25299 T25288 conj terminus,terminus
R7257 T25300 T25299 punct -,terminus
R7258 T25301 T25302 punct (,391
R7259 T25302 T25299 parataxis 391,terminus
R7260 T25303 T25302 nmod amino,391
R7261 T25304 T25302 nmod acids,391
R7262 T25305 T25306 punct –,542
R7263 T25306 T25302 prep 542,391
R7264 T25307 T25302 punct ),391
R7265 T25308 T25283 prep of,length
R7266 T25309 T25310 compound mr,s
R7267 T25310 T25308 pobj s,of
R7268 T25311 T25310 punct -,s
R7269 T25312 T25272 prep into,subcloned
R7270 T25313 T25314 nmod pGBKT7,vectors
R7271 T25314 T25312 pobj vectors,into
R7272 T25315 T25313 cc and,pGBKT7
R7273 T25316 T25313 conj pGADT7,pGBKT7
R7274 T25317 T25272 punct .,subcloned
R7275 T25319 T25320 nsubj We,assessed
R7276 T25321 T25320 dobj interactions,assessed
R7277 T25322 T25320 prep by,assessed
R7278 T25323 T25322 pcomp scoring,by
R7279 T25324 T25325 amod blue,color
R7280 T25325 T25323 dobj color,scoring
R7281 T25326 T25323 prep on,scoring
R7282 T25327 T25326 pobj plates,on
R7283 T25328 T25327 prep of,plates
R7284 T25329 T25328 pobj medium,of
R7285 T25330 T25327 acl containing,plates
R7286 T25331 T25332 compound X,gal
R7287 T25332 T25330 dobj gal,containing
R7288 T25333 T25332 punct -,gal
R7289 T25334 T25320 punct .,assessed