Id |
Subject |
Object |
Predicate |
Lexical cue |
T24541 |
0-5 |
JJ |
denotes |
Total |
T24542 |
6-9 |
NN |
denotes |
RNA |
T24543 |
14-22 |
VBN |
denotes |
isolated |
T24544 |
10-13 |
VBD |
denotes |
was |
T24545 |
23-27 |
IN |
denotes |
from |
T24546 |
28-32 |
DT |
denotes |
each |
T24547 |
33-39 |
NN |
denotes |
tissue |
T24548 |
40-45 |
VBG |
denotes |
using |
T24549 |
46-52 |
NN |
denotes |
Trizol |
T24550 |
52-53 |
. |
denotes |
. |
T24551 |
53-131 |
sentence |
denotes |
A 1 μg of total RNA was reverse transcribed using SuperscriptII (Invitrogen). |
T24552 |
54-55 |
DT |
denotes |
A |
T24553 |
58-60 |
NN |
denotes |
μg |
T24554 |
56-57 |
CD |
denotes |
1 |
T24555 |
86-97 |
VBN |
denotes |
transcribed |
T24556 |
61-63 |
IN |
denotes |
of |
T24557 |
64-69 |
JJ |
denotes |
total |
T24558 |
70-73 |
NN |
denotes |
RNA |
T24559 |
74-77 |
VBD |
denotes |
was |
T24560 |
78-85 |
JJ |
denotes |
reverse |
T24561 |
98-103 |
VBG |
denotes |
using |
T24562 |
104-117 |
NNP |
denotes |
SuperscriptII |
T24563 |
118-119 |
-LRB- |
denotes |
( |
T24564 |
119-129 |
NNP |
denotes |
Invitrogen |
T24565 |
129-130 |
-RRB- |
denotes |
) |
T24566 |
130-131 |
. |
denotes |
. |
T24567 |
131-272 |
sentence |
denotes |
RT-PCR primers, which span introns, for detection of mr-s cDNA were 5'-TGTCCAGCCCAGCCAACCCAAGGAGACGACA-3' and 5'-TGTGGTCTCCTCATCAGTGAAGA-3'. |
T24568 |
132-134 |
NN |
denotes |
RT |
T24569 |
135-138 |
NN |
denotes |
PCR |
T24570 |
134-135 |
HYPH |
denotes |
- |
T24571 |
139-146 |
NNS |
denotes |
primers |
T24572 |
195-199 |
VBD |
denotes |
were |
T24573 |
146-148 |
, |
denotes |
, |
T24574 |
148-153 |
WDT |
denotes |
which |
T24575 |
154-158 |
VBP |
denotes |
span |
T24576 |
159-166 |
NNS |
denotes |
introns |
T24577 |
166-168 |
, |
denotes |
, |
T24578 |
168-171 |
IN |
denotes |
for |
T24579 |
172-181 |
NN |
denotes |
detection |
T24580 |
182-184 |
IN |
denotes |
of |
T24581 |
185-187 |
NN |
denotes |
mr |
T24582 |
188-189 |
NN |
denotes |
s |
T24583 |
187-188 |
HYPH |
denotes |
- |
T24584 |
190-194 |
NN |
denotes |
cDNA |
T24585 |
200-201 |
CD |
denotes |
5 |
T24586 |
203-234 |
NN |
denotes |
TGTCCAGCCCAGCCAACCCAAGGAGACGACA |
T24587 |
201-202 |
SYM |
denotes |
' |
T24588 |
202-203 |
HYPH |
denotes |
- |
T24589 |
234-235 |
HYPH |
denotes |
- |
T24590 |
235-236 |
CD |
denotes |
3 |
T24591 |
236-237 |
SYM |
denotes |
' |
T24592 |
238-241 |
CC |
denotes |
and |
T24593 |
242-243 |
CD |
denotes |
5 |
T24594 |
245-268 |
NN |
denotes |
TGTGGTCTCCTCATCAGTGAAGA |
T24595 |
243-244 |
SYM |
denotes |
' |
T24596 |
244-245 |
HYPH |
denotes |
- |
T24597 |
268-269 |
HYPH |
denotes |
- |
T24598 |
269-270 |
CD |
denotes |
3 |
T24599 |
270-271 |
SYM |
denotes |
' |
T24600 |
271-272 |
. |
denotes |
. |
T24601 |
272-356 |
sentence |
denotes |
Product size was 292 bp (positions 965–1256 of Genbank Accession Number #AY458844). |
T24602 |
273-280 |
NN |
denotes |
Product |
T24603 |
281-285 |
NN |
denotes |
size |
T24604 |
286-289 |
VBD |
denotes |
was |
T24605 |
290-293 |
CD |
denotes |
292 |
T24606 |
294-296 |
NN |
denotes |
bp |
T24607 |
297-298 |
-LRB- |
denotes |
( |
T24608 |
308-311 |
CD |
denotes |
965 |
T24609 |
298-307 |
NNS |
denotes |
positions |
T24610 |
311-312 |
SYM |
denotes |
– |
T24611 |
312-316 |
CD |
denotes |
1256 |
T24612 |
317-319 |
IN |
denotes |
of |
T24613 |
320-327 |
NNP |
denotes |
Genbank |
T24614 |
346-354 |
NN |
denotes |
AY458844 |
T24615 |
328-337 |
NNP |
denotes |
Accession |
T24616 |
338-344 |
NNP |
denotes |
Number |
T24617 |
345-346 |
SYM |
denotes |
# |
T24618 |
354-355 |
-RRB- |
denotes |
) |
T24619 |
355-356 |
. |
denotes |
. |
T24620 |
356-541 |
sentence |
denotes |
Primer pairs for mouse G3PDH were 5'-ACCACAGTCCATGCCATCAC-3' and 5'-TCCACCACCCTGTTGCTGTA- 3' which amplified a 452-bp product (positions 587–1038 of Genbank Accession Number #BC85275). |
T24621 |
357-363 |
NN |
denotes |
Primer |
T24622 |
364-369 |
NNS |
denotes |
pairs |
T24623 |
386-390 |
VBD |
denotes |
were |
T24624 |
370-373 |
IN |
denotes |
for |
T24625 |
374-379 |
NN |
denotes |
mouse |
T24626 |
380-385 |
NN |
denotes |
G3PDH |
T24627 |
391-392 |
CD |
denotes |
5 |
T24628 |
394-414 |
NN |
denotes |
ACCACAGTCCATGCCATCAC |
T24629 |
392-393 |
SYM |
denotes |
' |
T24630 |
393-394 |
HYPH |
denotes |
- |
T24631 |
414-415 |
HYPH |
denotes |
- |
T24632 |
415-416 |
CD |
denotes |
3 |
T24633 |
416-417 |
SYM |
denotes |
' |
T24634 |
418-421 |
CC |
denotes |
and |
T24635 |
422-423 |
CD |
denotes |
5 |
T24636 |
425-445 |
NN |
denotes |
TCCACCACCCTGTTGCTGTA |
T24637 |
423-424 |
SYM |
denotes |
' |
T24638 |
424-425 |
HYPH |
denotes |
- |
T24639 |
445-446 |
HYPH |
denotes |
- |
T24640 |
447-448 |
CD |
denotes |
3 |
T24641 |
448-449 |
SYM |
denotes |
' |
T24642 |
450-455 |
WDT |
denotes |
which |
T24643 |
456-465 |
VBD |
denotes |
amplified |
T24644 |
466-467 |
DT |
denotes |
a |
T24645 |
475-482 |
NN |
denotes |
product |
T24646 |
468-471 |
CD |
denotes |
452 |
T24647 |
472-474 |
NN |
denotes |
bp |
T24648 |
471-472 |
HYPH |
denotes |
- |
T24649 |
483-484 |
-LRB- |
denotes |
( |
T24650 |
494-497 |
CD |
denotes |
587 |
T24651 |
484-493 |
NNS |
denotes |
positions |
T24652 |
497-498 |
SYM |
denotes |
– |
T24653 |
498-502 |
CD |
denotes |
1038 |
T24654 |
503-505 |
IN |
denotes |
of |
T24655 |
506-513 |
NNP |
denotes |
Genbank |
T24656 |
532-539 |
NN |
denotes |
BC85275 |
T24657 |
514-523 |
NN |
denotes |
Accession |
T24658 |
524-530 |
NN |
denotes |
Number |
T24659 |
531-532 |
SYM |
denotes |
# |
T24660 |
539-540 |
-RRB- |
denotes |
) |
T24661 |
540-541 |
. |
denotes |
. |
R6976 |
T24541 |
T24542 |
amod |
Total,RNA |
R6977 |
T24542 |
T24543 |
nsubjpass |
RNA,isolated |
R6978 |
T24544 |
T24543 |
auxpass |
was,isolated |
R6979 |
T24545 |
T24543 |
prep |
from,isolated |
R6980 |
T24546 |
T24547 |
det |
each,tissue |
R6981 |
T24547 |
T24545 |
pobj |
tissue,from |
R6982 |
T24548 |
T24543 |
advcl |
using,isolated |
R6983 |
T24549 |
T24548 |
dobj |
Trizol,using |
R6984 |
T24550 |
T24543 |
punct |
.,isolated |
R6985 |
T24552 |
T24553 |
det |
A,μg |
R6986 |
T24553 |
T24555 |
nsubjpass |
μg,transcribed |
R6987 |
T24554 |
T24553 |
nummod |
1,μg |
R6988 |
T24556 |
T24553 |
prep |
of,μg |
R6989 |
T24557 |
T24558 |
amod |
total,RNA |
R6990 |
T24558 |
T24556 |
pobj |
RNA,of |
R6991 |
T24559 |
T24555 |
auxpass |
was,transcribed |
R6992 |
T24560 |
T24555 |
amod |
reverse,transcribed |
R6993 |
T24561 |
T24555 |
advcl |
using,transcribed |
R6994 |
T24562 |
T24561 |
dobj |
SuperscriptII,using |
R6995 |
T24563 |
T24564 |
punct |
(,Invitrogen |
R6996 |
T24564 |
T24562 |
parataxis |
Invitrogen,SuperscriptII |
R6997 |
T24565 |
T24564 |
punct |
),Invitrogen |
R6998 |
T24566 |
T24555 |
punct |
.,transcribed |
R6999 |
T24568 |
T24569 |
compound |
RT,PCR |
R7000 |
T24569 |
T24571 |
compound |
PCR,primers |
R7001 |
T24570 |
T24569 |
punct |
-,PCR |
R7002 |
T24571 |
T24572 |
nsubj |
primers,were |
R7003 |
T24573 |
T24571 |
punct |
", ",primers |
R7004 |
T24574 |
T24575 |
dep |
which,span |
R7005 |
T24575 |
T24571 |
relcl |
span,primers |
R7006 |
T24576 |
T24575 |
dobj |
introns,span |
R7007 |
T24577 |
T24571 |
punct |
", ",primers |
R7008 |
T24578 |
T24571 |
prep |
for,primers |
R7009 |
T24579 |
T24578 |
pobj |
detection,for |
R7010 |
T24580 |
T24579 |
prep |
of,detection |
R7011 |
T24581 |
T24582 |
compound |
mr,s |
R7012 |
T24582 |
T24584 |
compound |
s,cDNA |
R7013 |
T24583 |
T24582 |
punct |
-,s |
R7014 |
T24584 |
T24580 |
pobj |
cDNA,of |
R7015 |
T24585 |
T24586 |
nummod |
5,TGTCCAGCCCAGCCAACCCAAGGAGACGACA |
R7016 |
T24586 |
T24572 |
attr |
TGTCCAGCCCAGCCAACCCAAGGAGACGACA,were |
R7017 |
T24587 |
T24585 |
punct |
',5 |
R7018 |
T24588 |
T24586 |
punct |
-,TGTCCAGCCCAGCCAACCCAAGGAGACGACA |
R7019 |
T24589 |
T24586 |
punct |
-,TGTCCAGCCCAGCCAACCCAAGGAGACGACA |
R7020 |
T24590 |
T24586 |
nummod |
3,TGTCCAGCCCAGCCAACCCAAGGAGACGACA |
R7021 |
T24591 |
T24586 |
punct |
',TGTCCAGCCCAGCCAACCCAAGGAGACGACA |
R7022 |
T24592 |
T24586 |
cc |
and,TGTCCAGCCCAGCCAACCCAAGGAGACGACA |
R7023 |
T24593 |
T24594 |
nummod |
5,TGTGGTCTCCTCATCAGTGAAGA |
R7024 |
T24594 |
T24586 |
conj |
TGTGGTCTCCTCATCAGTGAAGA,TGTCCAGCCCAGCCAACCCAAGGAGACGACA |
R7025 |
T24595 |
T24593 |
punct |
',5 |
R7026 |
T24596 |
T24594 |
punct |
-,TGTGGTCTCCTCATCAGTGAAGA |
R7027 |
T24597 |
T24594 |
punct |
-,TGTGGTCTCCTCATCAGTGAAGA |
R7028 |
T24598 |
T24594 |
nummod |
3,TGTGGTCTCCTCATCAGTGAAGA |
R7029 |
T24599 |
T24594 |
punct |
',TGTGGTCTCCTCATCAGTGAAGA |
R7030 |
T24600 |
T24572 |
punct |
.,were |
R7031 |
T24602 |
T24603 |
compound |
Product,size |
R7032 |
T24603 |
T24604 |
nsubj |
size,was |
R7033 |
T24605 |
T24606 |
nummod |
292,bp |
R7034 |
T24606 |
T24604 |
attr |
bp,was |
R7035 |
T24607 |
T24608 |
punct |
(,965 |
R7036 |
T24608 |
T24604 |
parataxis |
965,was |
R7037 |
T24609 |
T24608 |
nmod |
positions,965 |
R7038 |
T24610 |
T24611 |
punct |
–,1256 |
R7039 |
T24611 |
T24608 |
prep |
1256,965 |
R7040 |
T24612 |
T24608 |
prep |
of,965 |
R7041 |
T24613 |
T24614 |
nmod |
Genbank,AY458844 |
R7042 |
T24614 |
T24612 |
pobj |
AY458844,of |
R7043 |
T24615 |
T24614 |
nmod |
Accession,AY458844 |
R7044 |
T24616 |
T24614 |
nmod |
Number,AY458844 |
R7045 |
T24617 |
T24614 |
punct |
#,AY458844 |
R7046 |
T24618 |
T24608 |
punct |
),965 |
R7047 |
T24619 |
T24604 |
punct |
.,was |
R7048 |
T24621 |
T24622 |
compound |
Primer,pairs |
R7049 |
T24622 |
T24623 |
nsubj |
pairs,were |
R7050 |
T24624 |
T24622 |
prep |
for,pairs |
R7051 |
T24625 |
T24626 |
compound |
mouse,G3PDH |
R7052 |
T24626 |
T24624 |
pobj |
G3PDH,for |
R7053 |
T24627 |
T24628 |
nummod |
5,ACCACAGTCCATGCCATCAC |
R7054 |
T24628 |
T24623 |
attr |
ACCACAGTCCATGCCATCAC,were |
R7055 |
T24629 |
T24627 |
punct |
',5 |
R7056 |
T24630 |
T24628 |
punct |
-,ACCACAGTCCATGCCATCAC |
R7057 |
T24631 |
T24628 |
punct |
-,ACCACAGTCCATGCCATCAC |
R7058 |
T24632 |
T24628 |
nummod |
3,ACCACAGTCCATGCCATCAC |
R7059 |
T24633 |
T24628 |
punct |
',ACCACAGTCCATGCCATCAC |
R7060 |
T24634 |
T24628 |
cc |
and,ACCACAGTCCATGCCATCAC |
R7061 |
T24635 |
T24636 |
nummod |
5,TCCACCACCCTGTTGCTGTA |
R7062 |
T24636 |
T24628 |
conj |
TCCACCACCCTGTTGCTGTA,ACCACAGTCCATGCCATCAC |
R7063 |
T24637 |
T24635 |
punct |
',5 |
R7064 |
T24638 |
T24636 |
punct |
-,TCCACCACCCTGTTGCTGTA |
R7065 |
T24639 |
T24636 |
punct |
-,TCCACCACCCTGTTGCTGTA |
R7066 |
T24640 |
T24636 |
nummod |
3,TCCACCACCCTGTTGCTGTA |
R7067 |
T24641 |
T24636 |
punct |
',TCCACCACCCTGTTGCTGTA |
R7068 |
T24642 |
T24643 |
dep |
which,amplified |
R7069 |
T24643 |
T24628 |
relcl |
amplified,ACCACAGTCCATGCCATCAC |
R7070 |
T24644 |
T24645 |
det |
a,product |
R7071 |
T24645 |
T24643 |
dobj |
product,amplified |
R7072 |
T24646 |
T24647 |
nummod |
452,bp |
R7073 |
T24647 |
T24645 |
compound |
bp,product |
R7074 |
T24648 |
T24647 |
punct |
-,bp |
R7075 |
T24649 |
T24650 |
punct |
(,587 |
R7076 |
T24650 |
T24643 |
parataxis |
587,amplified |
R7077 |
T24651 |
T24650 |
nmod |
positions,587 |
R7078 |
T24652 |
T24653 |
punct |
–,1038 |
R7079 |
T24653 |
T24650 |
prep |
1038,587 |
R7080 |
T24654 |
T24650 |
prep |
of,587 |
R7081 |
T24655 |
T24656 |
nmod |
Genbank,BC85275 |
R7082 |
T24656 |
T24654 |
pobj |
BC85275,of |
R7083 |
T24657 |
T24656 |
nmod |
Accession,BC85275 |
R7084 |
T24658 |
T24656 |
nmod |
Number,BC85275 |
R7085 |
T24659 |
T24656 |
punct |
#,BC85275 |
R7086 |
T24660 |
T24650 |
punct |
),587 |
R7087 |
T24661 |
T24623 |
punct |
.,were |