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PMC:1435744 JSONTXT

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craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T895 0-7 SO_EXT:sequence_cloning_process denotes Cloning
T896 32-36 PR_EXT:000014441 denotes mr-s
T897 50-56 SO_EXT:0000417 denotes domain
T898 57-64 CHEBI_PR_EXT:protein denotes protein
T899 80-89 GO:0010467 denotes expressed
T900 93-100 UBERON:0000966 denotes retinal
T901 93-120 CL:0010009 denotes retinal photoreceptor cells
T902 115-120 CL_GO_EXT:cell denotes cells
T903 156-161 SO_EXT:sequence_or_structure_motif denotes motif
T904 168-175 SO_EXT:0000417 denotes domains
T905 184-192 CHEBI_EXT:residue denotes residues
T906 231-238 CHEBI_PR_EXT:protein denotes protein
T907 239-246 CHEBI_PR_EXT:protein denotes protein
T908 259-266 SO_EXT:0000417 denotes modules
T909 273-279 SO_EXT:0000417 denotes domain
T910 310-318 CHEBI_PR_EXT:protein denotes proteins
T911 351-359 CHEBI_PR_EXT:protein denotes proteins
T912 375-388 GO_EXT:transcription denotes transcription
T913 375-396 GO_EXT:transcription_factor denotes transcription factors
T914 426-433 SO_EXT:sequence_cloning_process denotes cloning
T915 481-487 SO_EXT:0000417 denotes domain
T916 499-506 CHEBI_PR_EXT:protein denotes protein
T917 531-540 GO:0010467 denotes expressed
T918 544-551 UBERON:0000966 denotes retinal
T919 544-566 CL:0010009 denotes retinal photoreceptors
T920 575-587 UBERON:0001905 denotes pineal gland
T921 606-611 NCBITaxon:10088 denotes mouse
T922 612-616 PR_EXT:000014441 denotes mr-s
T923 618-650 PR_EXT:000014441 denotes major retinal SAM domain protein
T924 624-631 UBERON:0000966 denotes retinal
T925 636-642 SO_EXT:0000417 denotes domain
T926 643-650 CHEBI_PR_EXT:protein denotes protein
T927 662-666 PR_EXT:000014441 denotes mr-s
T928 670-694 SO_EXT:biological_conservation_process_or_quality denotes evolutionarily conserved
T929 700-709 NCBITaxon:7955 denotes zebrafish
T930 718-723 NCBITaxon:9606 denotes human
T931 725-734 NCBITaxon:1 denotes organisms
T932 766-779 CL:0000210 denotes photoreceptor
T933 766-791 GO:0042461 denotes photoreceptor development
T934 807-816 SO_EXT:biological_conservation_process_or_quality denotes conserved
T935 862-868 SO_EXT:0000417 denotes domain
T936 872-876 PR_EXT:000014441 denotes mr-s
T937 906-911 NCBITaxon:10088 denotes mouse
T938 930-938 SO_EXT:0000855 denotes ortholog
T939 940-944 PR_EXT:Q64028 denotes Mph1
T940 945-950 PR_EXT:000012633 denotes Rae28
T941 973-983 SO_EXT:epigenetic_modification_entity_or_process denotes epigenetic
T942 984-992 CHEBI:36357 denotes molecule
T943 1005-1014 GO:0000785 denotes chromatin
T944 1005-1028 GO:0016568 denotes chromatin modifications
T945 1015-1028 SO_EXT:sequence_alteration_entity_or_process denotes modifications
T946 1074-1078 PR_EXT:000014441 denotes mr-s
T947 1107-1117 GO:0065007 denotes regulating
T948 1118-1122 SO_EXT:0000704 denotes gene
T949 1118-1133 GO:0010467 denotes gene expression
T950 1137-1150 CL:0000210 denotes photoreceptor
T951 1137-1162 GO:0042461 denotes photoreceptor development
T952 1164-1168 PR_EXT:000014441 denotes mr-s
T953 1187-1196 GO:0010467 denotes expressed
T954 1204-1218 CL:0000210 denotes photoreceptors
T955 1226-1231 GO:0007567 denotes natal
T956 1253-1267 CL:0000210 denotes photoreceptors
T957 1285-1300 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T958 1313-1318 UBERON:0007023 denotes adult
T959 1319-1331 UBERON:0001905 denotes pineal gland
T960 1333-1346 GO_EXT:transcription denotes Transcription
T961 1350-1354 PR_EXT:000014441 denotes mr-s
T962 1367-1376 GO:0065007 denotes regulated
T963 1384-1388 CL:0000573 denotes cone
T964 1389-1392 CL:0000604 denotes rod
T965 1405-1412 CHEBI_PR_EXT:protein denotes protein
T966 1413-1416 PR_EXT:000005904 denotes Crx
T967 1460-1464 PR_EXT:000014441 denotes mr-s
T968 1465-1472 CHEBI_PR_EXT:protein denotes protein
T969 1512-1518 SO_EXT:0000417 denotes domain
T970 1556-1560 PR_EXT:000014441 denotes mr-s
T971 1561-1568 CHEBI_PR_EXT:protein denotes protein
T972 1569-1578 GO_PATO_EXT:biological_localization_process_or_quality denotes localizes
T973 1593-1600 GO:0005634 denotes nucleus
T974 1607-1611 PR_EXT:000014441 denotes mr-s
T975 1619-1628 GO:0010467 denotes expressed
T976 1640-1645 CL_GO_EXT:cell denotes cells
T977 1664-1674 GO_EXT:0045289 denotes luciferase
T978 1697-1701 PR_EXT:000014441 denotes mr-s
T979 1702-1709 CHEBI_PR_EXT:protein denotes protein
T980 1710-1715 SO_EXT:sequence_fusion_process denotes fused
T981 1719-1723 PR_EXT:P04386 denotes GAL4
T982 1724-1727 CHEBI_SO_EXT:DNA denotes DNA
T983 1728-1735 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T984 1736-1742 SO_EXT:0000417 denotes domain
T985 1758-1773 GO_EXT:transcription denotes transcriptional
T986 1829-1833 PR_EXT:000014441 denotes mr-s
T987 1889-1895 SO_EXT:0000417 denotes domain
T988 1907-1917 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T989 1960-1964 SO_EXT:0000704 denotes gene
T990 1966-1970 PR_EXT:000014441 denotes mr-s
T991 1995-2004 GO:0010467 denotes expressed
T992 2008-2015 UBERON:0000966 denotes retinal
T993 2008-2030 CL:0010009 denotes retinal photoreceptors
T994 2035-2047 UBERON:0001905 denotes pineal gland
T995 2062-2072 GO:0010467 denotes expression
T996 2085-2096 CHEBI_EXT:biochemical denotes biochemical
T997 2123-2127 PR_EXT:000014441 denotes mr-s
T998 2146-2161 GO_EXT:transcription denotes transcriptional
T999 2175-2194 CL:0000210 denotes photoreceptor cells
T1000 2189-2194 CL_GO_EXT:cell denotes cells
T1001 2202-2214 CL:0000652 denotes pinealocytes
T1002 2222-2234 UBERON:0001905 denotes pineal gland
T3386 2835-2843 CHEBI_PR_EXT:protein denotes proteins
T3387 2845-2854 PR_EXT:000001245 denotes rhodopsin
T3388 2858-2862 CL:0000604 denotes rods
T3389 2867-2871 CL:0000573 denotes cone
T3390 2872-2878 PR_EXT:000001119 denotes opsins
T3391 2882-2887 CL:0000573 denotes cones
T3392 2893-2901 CHEBI_PR_EXT:protein denotes proteins
T3393 2917-2934 GO:0007602 denotes phototransduction
T3394 2986-2996 GO:0005886 denotes membranous
T3395 3012-3025 GO:0001750 denotes outer segment
T3396 3031-3044 GO:0001750 denotes outer segment
T3397 3095-3103 GO_EXT:reaction_or_response denotes response
T3398 3133-3140 SO_EXT:0000704 denotes genetic
T3399 3141-3154 SO_EXT:sequence_alteration_entity_or_process denotes perturbations
T3400 3163-3169 NCBITaxon:9989 denotes rodent
T3401 3170-3176 UBERON:0000966 denotes retina
T3402 3205-3209 CL_GO_EXT:cell denotes cell
T3403 3272-3289 CL:0000604 denotes Rod photoreceptor
T3404 3272-3300 GO:0046548 denotes Rod photoreceptor generation
T3405 3326-3331 GO:0007567 denotes birth
T3406 3333-3352 CL:0000573 denotes Cone photoreceptors
T3407 3354-3362 UBERON:0000045 denotes ganglion
T3408 3354-3368 CL:0000740 denotes ganglion cells
T3409 3363-3368 CL_GO_EXT:cell denotes cells
T3410 3370-3386 CL:0000745 denotes horizontal cells
T3411 3381-3386 CL_GO_EXT:cell denotes cells
T3412 3391-3405 CL:0000561 denotes amacrine cells
T3413 3400-3405 CL_GO_EXT:cell denotes cells
T3414 3435-3446 CL:0011107 denotes Müller glia
T3415 3451-3464 CL:0000103 denotes bipolar cells
T3416 3459-3464 CL_GO_EXT:cell denotes cells
T3417 3515-3519 CL_GO_EXT:cell denotes cell
T3418 3622-3627 CL_GO_EXT:cell denotes cells
T3419 3644-3657 GO_EXT:transcription denotes transcription
T3420 3661-3670 GO:0000785 denotes chromatin
T3421 3661-3683 GO:0016568 denotes chromatin modification
T3422 3671-3683 SO_EXT:sequence_alteration_entity_or_process denotes modification
T3423 3729-3742 GO_EXT:transcription denotes transcription
T3424 3729-3750 GO_EXT:transcription_factor denotes transcription factors
T3425 3754-3767 CL:0000210 denotes photoreceptor
T3426 3754-3779 GO:0042461 denotes photoreceptor development
T3427 3791-3794 PR_EXT:OTX denotes Otx
T3428 3811-3816 SO_EXT:0000704 denotes genes
T3429 3818-3822 PR_EXT:000012086 denotes Otx2
T3430 3827-3830 PR_EXT:000005904 denotes Crx
T3431 3862-3866 CL_GO_EXT:cell denotes cell
T3432 3862-3885 _FRAGMENT denotes cell fate determination
T3433 3915-3932 GO:0043703 denotes of photoreceptors
T3434 3899-3914 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T3435 3918-3932 CL:0000210 denotes photoreceptors
T3436 3960-3964 PR_EXT:000012086 denotes Otx2
T3437 3975-3990 GO_RO_EXT:developmental_differentiation_process denotes differentiating
T3438 3991-4010 CL:0000210 denotes photoreceptor cells
T3439 4005-4010 CL_GO_EXT:cell denotes cells
T3440 4028-4036 CL:0000561 denotes amacrine
T3441 4042-4049 CL:0000540 denotes neurons
T3442 4055-4058 PR_EXT:000005904 denotes Crx
T3443 4083-4087 PR_EXT:000012086 denotes Otx2
T3444 4089-4097 GO:0065007 denotes controls
T3445 4102-4115 GO_EXT:transcription denotes transcription
T3446 4127-4145 CL:0000210 denotes photoreceptor cell
T3447 4141-4145 CL_GO_EXT:cell denotes cell
T3448 4155-4160 SO_EXT:0000704 denotes genes
T3449 4199-4213 GO:0001750 denotes outer segments
T3450 4215-4223 GO:0045202 denotes synaptic
T3451 4224-4233 GO_UBERON_EXT:nerve_ending_or_neuron_projection_terminus denotes terminals
T3452 4239-4256 GO:0007602 denotes phototransduction
T3453 4274-4277 PR_EXT:000005904 denotes Crx
T3454 4278-4289 SO_EXT:0000673 denotes transcripts
T3455 4302-4311 GO:0010467 denotes expressed
T3456 4326-4340 CL:0000210 denotes photoreceptors
T3457 4344-4353 UBERON:0000922 denotes embryonic
T3458 4378-4383 NCBITaxon:10088 denotes mouse
T3459 4397-4409 GO_EXT:positive_regulation denotes upregulation
T3460 4413-4416 PR_EXT:000005904 denotes Crx
T3461 4417-4430 GO_EXT:transcription denotes transcription
T3462 4454-4469 GO_RO_EXT:developmental_differentiation_process denotes differentiating
T3463 4470-4484 CL:0000210 denotes photoreceptors
T3464 4492-4497 GO:0007567 denotes natal
T3465 4510-4529 CL:0000210 denotes Photoreceptor cells
T3466 4524-4529 CL_GO_EXT:cell denotes cells
T3467 4537-4540 PR_EXT:000005904 denotes Crx
T3468 4555-4559 NCBITaxon:10088 denotes mice
T3469 4597-4610 CL:0000210 denotes photoreceptor
T3470 4611-4620 CHEBI:36357 denotes molecules
T3471 4631-4637 GO:0007601 denotes visual
T3472 4638-4646 CHEBI_EXT:26130 denotes pigments
T3473 4667-4680 CL:0000210 denotes photoreceptor
T3474 4667-4695 GO:0001750 denotes photoreceptor outer segments
T3475 4702-4710 GO:0045202 denotes synaptic
T3476 4711-4719 GO_UBERON_EXT:nerve_ending_or_neuron_projection_terminus denotes terminus
T3477 4741-4750 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T3478 4754-4759 NCBITaxon:9606 denotes human
T3479 4760-4763 PR_EXT:000005904 denotes CRX
T3480 4824-4837 CL:0000210 denotes photoreceptor
T3481 4848-4857 GO:0030849 denotes autosomal
T3482 4867-4871 CL:0000573 denotes cone
T3483 4872-4875 CL:0000604 denotes rod
T3484 4889-4898 GO:0030849 denotes autosomal
T3485 4989-4993 PR_EXT:000012086 denotes Otx2
T3486 4998-5001 PR_EXT:000005904 denotes Crx
T3487 5002-5009 GO:0065007 denotes control
T3488 5018-5031 CL:0000210 denotes photoreceptor
T3489 5018-5043 GO:0042461 denotes photoreceptor development
T3490 5057-5070 GO_EXT:transcription denotes transcription
T3491 5057-5078 GO_EXT:transcription_factor denotes transcription factors
T3492 5080-5084 PR_EXT:000016321 denotes TRβ2
T3493 5086-5089 PR_EXT:000011432 denotes Nrl
T3494 5095-5100 PR_EXT:000011403 denotes Nr2e3
T3495 5101-5109 GO:0065007 denotes regulate
T3496 5131-5149 CL:0000210 denotes photoreceptor cell
T3497 5145-5149 CL_GO_EXT:cell denotes cell
T3498 5169-5176 SO_EXT:0000417 denotes domains
T3499 5213-5220 SO_EXT:0000417 denotes domains
T3500 5230-5238 CHEBI_EXT:residue denotes residues
T3501 5277-5284 CHEBI_PR_EXT:protein denotes protein
T3502 5285-5292 CHEBI_PR_EXT:protein denotes protein
T3503 5305-5312 SO_EXT:0000417 denotes modules
T3504 5327-5333 SO_EXT:0000417 denotes domain
T3505 5364-5372 CHEBI_PR_EXT:protein denotes proteins
T3506 5405-5413 CHEBI_PR_EXT:protein denotes proteins
T3507 5420-5426 CHEBI_SO_EXT:serine denotes serine
T3508 5420-5444 GO_EXT:0004674 denotes serine threonine kinases
T3509 5427-5436 CHEBI_SO_EXT:threonine denotes threonine
T3510 5451-5470 GO_PR_EXT:ephrin_receptor denotes Eph family receptor
T3511 5451-5487 GO_PR_EXT:ephrin_receptor denotes Eph family receptor tyrosine kinases
T3512 5462-5487 GO_EXT:0004714 denotes receptor tyrosine kinases
T3513 5471-5479 CHEBI_SO_EXT:tyrosine denotes tyrosine
T3514 5498-5501 PR_EXT:000016568 denotes p73
T3515 5529-5532 CHEBI_SO_EXT:RNA denotes RNA
T3516 5533-5540 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T3517 5541-5548 CHEBI_PR_EXT:protein denotes protein
T3518 5549-5554 PR_EXT:Q23972 denotes Smaug
T3519 5561-5575 CHEBI:18035 denotes diacylglycerol
T3520 5561-5583 GO_EXT:0004143 denotes diacylglycerol kinases
T3521 5593-5598 NCBITaxon_EXT:yeast denotes yeast
T3522 5599-5605 GO:0007618 denotes mating
T3523 5611-5620 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling
T3524 5621-5629 CHEBI_PR_EXT:protein denotes proteins
T3525 5653-5666 GO_EXT:transcription denotes transcription
T3526 5653-5674 GO_EXT:transcription_factor denotes transcription factors
T3527 5692-5700 CHEBI_PR_EXT:protein denotes proteins
T3528 5705-5720 GO_EXT:transcription denotes transcriptional
T3529 5746-5750 SO_EXT:0000704 denotes gene
T3530 5746-5760 GO:0016458 denotes gene silencing
T3531 5792-5799 NCBITaxon:40674 denotes mammals
T3532 5805-5813 CHEBI_PR_EXT:protein denotes proteins
T3533 5841-5845 SO_EXT:0000704 denotes gene
T3534 5846-5856 GO:0065007 denotes regulation
T3535 5864-5874 CHEBI_GO_EXT:biological_process_or_quality_or_role denotes biological
T3536 5899-5911 _FRAGMENT denotes silencing of
T3537 5929-5934 GO:0016458 denotes genes
T3538 5929-5934 SO_EXT:0000704 denotes genes
T3539 5943-5952 GO:0000785 denotes chromatin
T3540 5943-5966 GO:0016568 denotes chromatin modifications
T3541 5953-5966 SO_EXT:sequence_alteration_entity_or_process denotes modifications
T3542 5980-6009 GO:0035102 denotes polycomb repressive complex 1
T3543 6011-6015 GO:0035102 denotes PRC1
T3544 6037-6043 SO_EXT:0000417 denotes domain
T3545 6051-6061 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T3546 6067-6079 GO:0035102 denotes PRC1 complex
T3547 6097-6104 SO_EXT:0001114 denotes helical
T3548 6119-6127 CHEBI:33839 denotes polymers
T3549 6144-6150 SO_EXT:0000417 denotes domain
T3550 6163-6172 CHEBI:33839 denotes polymeric
T3551 6224-6233 GO:0000785 denotes chromatin
T3552 6294-6299 SO_EXT:0000704 denotes genes
T3553 6338-6342 SO_EXT:0000704 denotes gene
T3554 6344-6348 PR_EXT:000014441 denotes mr-s
T3555 6356-6363 SO_EXT:sequence_coding_function denotes encodes
T3556 6370-6376 SO_EXT:0000417 denotes domain
T3557 6388-6395 CHEBI_PR_EXT:protein denotes protein
T3558 6405-6411 SO_EXT:0000417 denotes domain
T3559 6415-6419 PR_EXT:000014441 denotes mr-s
T3560 6458-6463 NCBITaxon:10088 denotes mouse
T3561 6464-6472 SO_EXT:0000855 denotes ortholog
T3562 6474-6478 PR_EXT:Q64028 denotes MPH1
T3563 6479-6484 PR_EXT:000012633 denotes Rae28
T3564 6486-6490 PR_EXT:000014441 denotes mr-s
T3565 6508-6517 GO:0010467 denotes expressed
T3566 6521-6528 UBERON:0000966 denotes retinal
T3567 6521-6543 CL:0010009 denotes retinal photoreceptors
T3568 6571-6586 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T3569 6592-6597 UBERON:0007023 denotes adult
T3570 6598-6610 UBERON:0001905 denotes pineal gland
T3571 6616-6626 GO:0010467 denotes expression
T3572 6630-6634 PR_EXT:000014441 denotes mr-s
T3573 6647-6656 GO:0065007 denotes regulated
T3574 6660-6663 PR_EXT:000005904 denotes Crx
T3575 6675-6679 PR_EXT:000014441 denotes mr-s
T3576 6683-6692 GO_PATO_EXT:biological_localization_process_or_quality denotes localized
T3577 6700-6707 GO:0005634 denotes nucleus
T3578 6747-6753 SO_EXT:0000417 denotes domain
T3579 6792-6796 PR_EXT:000014441 denotes mr-s
T3580 6797-6804 CHEBI_PR_EXT:protein denotes protein
T3581 6805-6810 SO_EXT:sequence_fusion_process denotes fused
T3582 6814-6818 PR_EXT:P04386 denotes GAL4
T3583 6819-6822 CHEBI_SO_EXT:DNA denotes DNA
T3584 6823-6830 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T3585 6831-6837 SO_EXT:0000417 denotes domain
T3586 6853-6868 GO_EXT:transcription denotes transcriptional
T3587 6908-6912 PR_EXT:000014441 denotes mr-s
T3588 6940-6955 GO_EXT:transcription denotes transcriptional
T3589 6940-6973 GO:0017053 denotes transcriptional repressor complex
T3590 6977-6984 UBERON:0000966 denotes retinal
T3591 6977-6998 CL:0010009 denotes retinal photoreceptor
T3592 6985-7010 GO:0042461 denotes photoreceptor development
T5440 7022-7029 SO_EXT:sequence_cloning_process denotes Cloning
T5441 7033-7038 NCBITaxon:10088 denotes mouse
T5442 7039-7043 PR_EXT:000014441 denotes mr-s
T5443 7071-7076 NCBITaxon:10088 denotes mouse
T5444 7077-7082 SO_EXT:0000704 denotes genes
T5445 7098-7107 GO:0010467 denotes expressed
T5446 7126-7132 UBERON:0000966 denotes retina
T5447 7276-7279 SO_EXT:0000345 denotes EST
T5448 7322-7327 NCBITaxon:10088 denotes mouse
T5449 7328-7335 UBERON:0000966 denotes retinal
T5450 7336-7340 SO_EXT:cDNA denotes cDNA
T5451 7370-7375 SO_EXT:sequence_cloned_entity denotes clone
T5452 7385-7390 SO_EXT:cDNA denotes cDNAs
T5453 7391-7398 SO_EXT:sequence_coding_function denotes encodes
T5454 7401-7408 CHEBI_PR_EXT:protein denotes protein
T5455 7426-7432 SO_EXT:0000417 denotes domain
T5456 7465-7472 CHEBI_PR_EXT:protein denotes protein
T5457 7511-7516 NCBITaxon:10088 denotes mouse
T5458 7517-7522 SO_EXT:sequence_cloned_entity denotes clone
T5459 7544-7549 NCBITaxon:10088 denotes mouse
T5460 7556-7563 UBERON:0000966 denotes retinal
T5461 7564-7568 SO_EXT:cDNA denotes cDNA
T5462 7601-7611 SO_EXT:0000317 denotes cDNA clone
T5463 7613-7621 SO_EXT:biological_sequence denotes Sequence
T5464 7648-7652 SO_EXT:cDNA denotes cDNA
T5465 7665-7669 SO_EXT:0000704 denotes gene
T5466 7670-7678 SO_EXT:sequence_coding_function denotes encoding
T5467 7685-7691 SO_EXT:0000417 denotes domain
T5468 7703-7710 CHEBI_PR_EXT:protein denotes protein
T5469 7732-7739 CHEBI_PR_EXT:protein denotes protein
T5470 7743-7747 PR_EXT:000014441 denotes mr-s
T5471 7749-7781 PR_EXT:000014441 denotes major retinal SAM domain protein
T5472 7755-7762 UBERON:0000966 denotes retinal
T5473 7767-7773 SO_EXT:0000417 denotes domain
T5474 7774-7781 CHEBI_PR_EXT:protein denotes protein
T5475 7807-7829 GO:0006413 denotes translation initiation
T5476 7807-7835 SO_EXT:0000318 denotes translation initiation codon
T5477 7859-7877 SO_EXT:0000236 denotes open reading frame
T5478 7889-7895 SO_EXT:0000417 denotes domain
T5479 7902-7917 SO_EXT:0000318 denotes initiation site
T5480 7942-7951 SO:0000993 denotes consensus
T5481 7952-7960 SO_EXT:biological_sequence denotes sequence
T5482 8021-8030 SO_EXT:biological_conservation_process_or_quality denotes conserved
T5483 8031-8037 CHEBI_EXT:purine denotes purine
T5484 8058-8068 SO_EXT:0000319 denotes stop codon
T5485 8086-8090 PR_EXT:000014441 denotes mr-s
T5486 8091-8098 CHEBI_PR_EXT:protein denotes protein
T5487 8133-8143 CHEBI_SO_EXT:amino_acid denotes amino acid
T5488 8144-8152 SO_EXT:biological_sequence denotes sequence
T5489 8164-8170 SO_EXT:0000417 denotes domain
T5490 8174-8178 PR_EXT:000014441 denotes mr-s
T5491 8179-8186 CHEBI_PR_EXT:protein denotes protein
T5492 8203-8211 SO_EXT:biological_sequence denotes sequence
T5493 8222-8230 SO:0000857 denotes homology
T5494 8240-8247 SO_EXT:0000417 denotes domains
T5495 8251-8256 PR_EXT:000007130 denotes EphB2
T5496 8258-8263 PR_EXT:000007124 denotes EphA4
T5497 8265-8269 PR_EXT:Q64028 denotes MPH1
T5498 8271-8274 PR_EXT:000007229 denotes TEL
T5499 8279-8284 PR_EXT:Q23972 denotes Smaug
T5500 8330-8336 SO_EXT:0000417 denotes domain
T5501 8340-8344 PR_EXT:000014441 denotes mr-s
T5502 8380-8384 PR_EXT:Q64028 denotes Mph1
T5503 8385-8390 PR_EXT:000012633 denotes Rae28
T5504 8394-8399 NCBITaxon:10088 denotes mouse
T5505 8400-8407 SO_EXT:0000853 denotes homolog
T5506 8425-8430 NCBITaxon:10088 denotes Mouse
T5507 8431-8435 PR_EXT:000014441 denotes mr-s
T5508 8436-8443 CHEBI_PR_EXT:protein denotes protein
T5509 8447-8456 SO_EXT:biological_conservation_process_or_quality denotes conserved
T5510 8460-8463 NCBITaxon:10114 denotes rat
T5511 8465-8470 NCBITaxon:9606 denotes human
T5512 8472-8477 NCBITaxon_UBERON_EXT:chick denotes chick
T5513 8482-8491 NCBITaxon:7955 denotes zebrafish
T5514 8543-8548 NCBITaxon:10088 denotes mouse
T5515 8549-8553 PR_EXT:000014441 denotes mr-s
T5516 8587-8594 SO_EXT:0000417 denotes domains
T5517 8598-8601 NCBITaxon:10114 denotes rat
T5518 8603-8608 NCBITaxon:9606 denotes human
T5519 8610-8615 NCBITaxon_UBERON_EXT:chick denotes chick
T5520 8620-8629 NCBITaxon:7955 denotes zebrafish
T5521 8630-8634 PR_EXT:000014441 denotes mr-s
T5522 8635-8642 CHEBI_PR_EXT:protein denotes protein
T5523 8654-8663 SO_EXT:biological_conservation_process_or_quality denotes conserved
T5524 8720-8726 SO_EXT:0000417 denotes domain
T5525 8730-8735 NCBITaxon:10088 denotes mouse
T5526 8736-8740 PR_EXT:000014441 denotes mr-s
T5527 8741-8748 CHEBI_PR_EXT:protein denotes protein
T5528 8754-8765 GO_SO_EXT:chromosome denotes chromosomal
T5529 8766-8779 GO_PATO_EXT:biological_localization_process_or_quality denotes localizations
T5530 8783-8788 NCBITaxon:10088 denotes mouse
T5531 8793-8798 NCBITaxon:9606 denotes human
T5532 8799-8803 PR_EXT:000014441 denotes mr-s
T5533 8804-8809 SO_EXT:0000704 denotes genes
T5534 8843-8848 NCBITaxon:10088 denotes mouse
T5535 8853-8858 NCBITaxon:9606 denotes human
T5536 8859-8865 SO_EXT:0001026 denotes genome
T5537 8898-8903 NCBITaxon:10088 denotes Mouse
T5538 8904-8908 PR_EXT:000014441 denotes mr-s
T5539 8922-8932 GO_SO_EXT:chromosome denotes chromosome
T5540 8942-8947 NCBITaxon:9606 denotes human
T5541 8948-8952 PR_EXT:000014441 denotes MR-S
T5542 8966-8976 GO_SO_EXT:chromosome denotes chromosome
T5543 9028-9037 UBERON_EXT:childhood denotes childhood
T5544 9049-9054 NCBITaxon:9606 denotes Human
T5545 9055-9059 PR_EXT:000014441 denotes MR-S
T7242 10257-10267 GO:0010467 denotes Expression
T7243 10271-10275 PR_EXT:000014441 denotes mr-s
T7244 10294-10300 UBERON:0000966 denotes retina
T7245 10309-10321 UBERON:0001905 denotes pineal gland
T7246 10337-10341 PR_EXT:000014441 denotes mr-s
T7247 10342-10352 GO:0010467 denotes expression
T7248 10393-10406 GO:0097617 denotes hybridization
T7249 10410-10415 NCBITaxon:10088 denotes mouse
T7250 10416-10423 UBERON:0000922 denotes embryos
T7251 10428-10441 GO:0097617 denotes hybridization
T7252 10495-10499 PR_EXT:000014441 denotes mr-s
T7253 10500-10505 CHEBI_SO_EXT:molecular_probe denotes probe
T7254 10549-10559 GO:0010467 denotes expression
T7255 10567-10571 PR_EXT:000014441 denotes mr-s
T7256 10572-10576 SO_EXT:0000704 denotes gene
T7257 10595-10601 UBERON:0000966 denotes retina
T7258 10621-10634 GO:0097617 denotes hybridization
T7259 10701-10707 UBERON:0000966 denotes retina
T7260 10790-10802 CL:0000031 denotes neuroblastic
T7261 10830-10843 CL:0000210 denotes photoreceptor
T7262 10830-10849 UBERON:0001787 denotes photoreceptor layer
T7263 10885-10889 PR_EXT:000014441 denotes mr-s
T7301 12498-12503 UBERON:0007023 denotes adult
T7302 12504-12511 UBERON:0000479 denotes tissues
T7303 12537-12550 GO:0097617 denotes hybridization
T7304 12579-12586 UBERON:0000966 denotes retinal
T7305 12587-12590 CHEBI_SO_EXT:RNA denotes RNA
T7306 12634-12635 CHEBI_SO_EXT:base denotes b
T7307 12642-12643 CHEBI_SO_EXT:base denotes b
T7308 12650-12651 CHEBI_SO_EXT:base denotes b
T7309 12661-12662 CHEBI_SO_EXT:base denotes b
T7310 12683-12689 UBERON:0000966 denotes retina
T7311 12700-12701 CHEBI_SO_EXT:base denotes b
T7312 12726-12730 SO_EXT:cDNA denotes cDNA
T7313 12787-12806 GO:0000380 denotes alternative spliced
T7314 12787-12818 SO_EXT:1001187 denotes alternative spliced transcripts
T7315 12857-12861 PR_EXT:000014441 denotes mr-s
T7316 12862-12867 CHEBI_SO_EXT:molecular_probe denotes probe
T7317 12897-12902 UBERON:0007023 denotes adult
T7318 12903-12910 UBERON:0000479 denotes tissues
T7319 12943-12950 UBERON:0000479 denotes tissues
T7320 12958-12965 GO:0010467 denotes express
T7321 12966-12970 PR_EXT:000014441 denotes mr-s
T7322 13019-13025 UBERON:0000966 denotes retina
T7323 13063-13076 CL:0000210 denotes photoreceptor
T7324 13086-13091 SO_EXT:0000704 denotes genes
T7325 13101-13110 GO:0010467 denotes expressed
T7326 13118-13130 UBERON:0001905 denotes pineal gland
T7327 13153-13163 GO:0010467 denotes expression
T7328 13167-13171 PR_EXT:000014441 denotes mr-s
T7329 13172-13183 SO_EXT:0000673 denotes transcripts
T7330 13197-13203 UBERON:0000922 denotes embryo
T7331 13205-13215 UBERON:0000468 denotes whole body
T7332 13217-13223 UBERON:0000966 denotes retina
T7333 13225-13237 UBERON:0001905 denotes pineal gland
T7334 13239-13244 UBERON:0000955 denotes brain
T7335 13246-13251 UBERON:0002107 denotes liver
T7336 13262-13268 UBERON:0000062 denotes organs
T7337 13290-13292 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T7338 13344-13353 _FRAGMENT denotes fragments
T7339 13393-13402 SO_EXT:0000997 denotes for genes
T7340 13361-13363 SO_EXT:0000028 denotes bp
T7341 13372-13374 SO_EXT:0000028 denotes bp
T7342 13380-13386 SO_EXT:0000112 denotes primer
T7343 13403-13411 SO_EXT:sequence_coding_function denotes encoding
T7344 13412-13417 NCBITaxon:10088 denotes mouse
T7345 13418-13422 PR_EXT:000014441 denotes mr-s
T7346 13427-13432 GO_PR_EXT:glyceraldehyde_3_phosphate_dehydrogenase denotes G3PDH
T7347 13461-13467 UBERON:0000922 denotes embryo
T7348 13475-13485 UBERON:0000468 denotes whole body
T7349 13502-13505 UBERON:0000970 denotes eye
T7350 13511-13515 PR_EXT:000014441 denotes mr-s
T7351 13567-13571 PR_EXT:000014441 denotes mr-s
T7352 13575-13584 GO:0010467 denotes expressed
T7353 13599-13604 UBERON:0007023 denotes adult
T7354 13605-13617 UBERON:0001905 denotes pineal gland
T7355 13633-13638 UBERON:0007023 denotes adult
T7356 13639-13644 UBERON:0000955 denotes brain
T7357 13646-13651 UBERON:0002107 denotes liver
T7358 13670-13676 UBERON:0000062 denotes organs
T7359 13682-13684 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T7360 13707-13711 PR_EXT:000014441 denotes mr-s
T7361 13780-13784 PR_EXT:000014441 denotes mr-s
T7362 13802-13811 GO:0010467 denotes expressed
T7363 13826-13840 CL:0000210 denotes photoreceptors
T7364 13849-13861 UBERON:0001905 denotes pineal gland
T9532 13864-13874 GO:0065007 denotes Regulation
T9533 13878-13882 PR_EXT:000014441 denotes mr-s
T9534 13883-13896 GO_EXT:transcription denotes transcription
T9535 13900-13903 PR_EXT:000005904 denotes Crx
T9536 13916-13923 CHEBI_PR_EXT:protein denotes protein
T9537 13924-13937 GO_EXT:transcription denotes Transcription
T9538 13924-13945 GO_EXT:transcription_factor denotes Transcription factors
T9539 13946-13950 PR_EXT:000012086 denotes Otx2
T9540 13955-13958 PR_EXT:000005904 denotes Crx
T9541 13979-13989 GO_EXT:regulator denotes regulators
T9542 13993-14000 UBERON:0000966 denotes retinal
T9543 13993-14014 CL:0010009 denotes retinal photoreceptor
T9544 14001-14026 GO:0042461 denotes photoreceptor development
T9545 14050-14054 PR_EXT:000012086 denotes Otx2
T9546 14059-14062 PR_EXT:000005904 denotes Crx
T9547 14067-14076 GO:0010467 denotes expressed
T9548 14091-14110 CL:0000210 denotes photoreceptor cells
T9549 14105-14110 CL_GO_EXT:cell denotes cells
T9550 14118-14127 UBERON:0000922 denotes embryonic
T9551 14128-14134 UBERON:0000966 denotes retina
T9552 14141-14144 PR_EXT:000005904 denotes Crx
T9553 14155-14164 GO:0010467 denotes expressed
T9554 14176-14181 GO:0007567 denotes natal
T9555 14182-14196 CL:0000210 denotes photoreceptors
T9582 14695-14699 PR_EXT:000014441 denotes mr-s
T9583 14707-14716 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9584 14721-14724 PR_EXT:000005904 denotes Crx
T9585 14728-14740 UBERON:0001905 denotes pineal gland
T9586 14778-14782 PR_EXT:000014441 denotes mr-s
T9587 14783-14796 GO_EXT:transcription denotes transcription
T9588 14815-14828 GO_EXT:negative_regulation denotes downregulated
T9589 14836-14839 PR_EXT:000005904 denotes Crx
T9590 14843-14855 UBERON:0001905 denotes pineal gland
T9591 14898-14902 PR_EXT:000014441 denotes mr-s
T9592 14903-14916 GO_EXT:transcription denotes transcription
T9593 14920-14929 GO:0065007 denotes regulated
T9594 14933-14936 PR_EXT:000005904 denotes Crx
T9595 14955-14969 CL:0000210 denotes photoreceptors
T9596 14974-14986 UBERON:0001905 denotes pineal gland
T9597 16030-16031 GO_EXT:transcription denotes o
T9598 16033-16034 GO:0065007 denotes u
T9599 16034-16035 PR_EXT:000005904 denotes r
T9600 16036-16037 GO:0097617 denotes h
T9601 16037-16038 CHEBI_SO_EXT:molecular_probe denotes e
T9602 16040-16041 NCBITaxon:10088 denotes e
T9603 16041-16042 PR_EXT:000005904 denotes x
T9604 16042-16043 UBERON:0000966 denotes a
T9605 16043-16044 PR_EXT:000014441 denotes m
T9606 16044-16046 GO:0010467 denotes in
T9607 16048-16049 SO_EXT:wild_type_entity_or_quality denotes w
T9608 16051-16052 SO_EXT:0000006 denotes t
T9609 16054-16055 SO_EXT:0000112 denotes r
T9610 16058-16059 PR_EXT:000005904 denotes x
T9611 16062-16063 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes g
T9612 16062-16063 SO_EXT:0000409 denotes g
T9613 16063-16064 GO_EXT:0045289 denotes u
T9614 16064-16065 PR_EXT:000011432 denotes l
T9624 16215-16219 SO_EXT:0000704 denotes gene
T9625 16225-16235 GO_EXT:0045289 denotes luciferase
T9626 16272-16275 PR_EXT:000005904 denotes Crx
T9627 16277-16281 PR_EXT:000012086 denotes Otx2
T9628 16283-16286 PR_EXT:000011432 denotes Nrl
T9629 16287-16297 GO:0010467 denotes expression
T9630 16298-16305 SO_EXT:0000440 denotes vectors
T9631 16331-16332 CHEBI_SO_EXT:base denotes b
T9632 16347-16351 PR_EXT:000014441 denotes mr-s
T9633 16352-16360 SO_EXT:sequence_upstreamness denotes upstream
T9634 16361-16369 SO_EXT:biological_sequence denotes sequence
T9635 16385-16388 PR_EXT:000005904 denotes Crx
T9636 16389-16396 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T9637 16397-16406 SO:0000993 denotes consensus
T9638 16407-16416 SO_EXT:biological_sequence denotes sequences
T9639 16443-16453 GO_EXT:0045289 denotes luciferase
T9640 16481-16492 GO_EXT:positive_regulation denotes upregulated
T9641 16502-16505 PR_EXT:000005904 denotes Crx
T9642 16509-16513 PR_EXT:000012086 denotes Otx2
T9643 16514-16524 GO:0010467 denotes expression
T9644 16525-16531 SO_EXT:0000440 denotes vector
T9645 16576-16581 CL_GO_EXT:cell denotes cells
T9646 16593-16603 GO_EXT:0045289 denotes luciferase
T9647 16638-16641 PR_EXT:000011432 denotes Nrl
T9648 16642-16652 GO:0010467 denotes expression
T9649 16653-16659 SO_EXT:0000440 denotes vector
T9650 16716-16731 GO_EXT:transcription denotes transcriptional
T9651 16744-16747 PR_EXT:000005904 denotes Crx
T9652 16766-16769 PR_EXT:000011432 denotes Nrl
T9653 16779-16788 PR_EXT:000001245 denotes rhodopsin
T9654 16789-16797 SO_EXT:0000167 denotes promoter
T9655 16821-16825 SO_EXT:0000704 denotes gene
T9656 16883-16893 GO_EXT:0045289 denotes luciferase
T9657 16894-16898 SO_EXT:0000704 denotes gene
T9658 16894-16909 GO:0010467 denotes gene expression
T9659 16918-16929 GO_EXT:positive_regulation denotes upregulated
T9660 16940-16943 PR_EXT:000005904 denotes Crx
T9661 16948-16951 PR_EXT:000011432 denotes Nrl
T9662 16952-16962 GO:0010467 denotes expression
T9663 16963-16970 SO_EXT:0000440 denotes vectors
T9664 17037-17040 PR_EXT:000005904 denotes Crx
T9665 17046-17056 GO:0010467 denotes expression
T9666 17057-17063 SO_EXT:0000440 denotes vector
T9667 17099-17103 CL_GO_EXT:cell denotes cell
T9668 17131-17135 CL_GO_EXT:cell denotes cell
T9669 17144-17151 UBERON:0000966 denotes retinal
T9670 17152-17158 UBERON:0001905 denotes pineal
T9671 17186-17196 GO_EXT:0045289 denotes luciferase
T9672 17246-17250 PR_EXT:000012086 denotes Otx2
T9673 17287-17294 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T9674 17295-17304 SO_EXT:0000994 denotes consensus
T9675 17308-17311 PR_EXT:000005904 denotes Crx
T9676 17333-17337 PR_EXT:000014441 denotes mr-s
T9677 17338-17348 GO:0010467 denotes expression
T9678 17377-17387 GO:0010467 denotes expression
T9679 17399-17403 PR_EXT:000014441 denotes mr-s
T9680 17428-17431 PR_EXT:000005904 denotes Crx
T9681 17450-17461 SO_EXT:0000673 denotes transcripts
T9682 17465-17469 PR_EXT:000012086 denotes Otx2
T9683 17497-17510 CL:0000210 denotes photoreceptor
T9684 17497-17516 UBERON:0001787 denotes photoreceptor layer
T9685 17520-17536 UBERON_EXT:embryonic_stage_or_stage_part denotes embryonic stages
T9686 17567-17571 PR_EXT:000014441 denotes mr-s
T9687 17572-17585 GO_EXT:transcription denotes transcription
T9688 17598-17607 GO:0065007 denotes regulated
T9689 17618-17621 PR_EXT:000005904 denotes Crx
T9690 17652-17659 SO_EXT:0000440 denotes vectors
T9691 17669-17678 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T9692 17708-17711 PR_EXT:000005904 denotes Crx
T9693 17712-17719 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T9694 17712-17725 SO_EXT:0000409 denotes binding sites
T9695 17760-17763 SO_EXT:sequence_altered_entity_or_alteration_process denotes mut
T9696 17779-17782 PR_EXT:000005904 denotes Crx
T9697 17783-17793 GO:0010467 denotes expression
T9698 17794-17800 SO_EXT:0000440 denotes vector
T9699 17851-17854 SO_EXT:sequence_altered_entity_or_alteration_process denotes mut
T9700 17915-17918 PR_EXT:000005904 denotes Crx
T9701 18018-18029 GO:0009294 denotes transfected
T9702 18063-18066 PR_EXT:000005904 denotes Crx
T9703 18115-18118 PR_EXT:000005904 denotes Crx
T9704 18119-18126 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T9705 18119-18132 SO_EXT:0000409 denotes binding sites
T9706 18136-18138 SO_EXT:0000028 denotes bp
T9707 18147-18149 SO_EXT:0000028 denotes bp
T9708 18150-18158 SO_EXT:sequence_upstreamness denotes upstream
T9709 18168-18181 GO_EXT:transcription denotes transcription
T9710 18168-18197 SO_EXT:0000315 denotes transcription initiation site
T9711 18225-18235 GO:0065007 denotes regulation
T9712 18239-18243 PR_EXT:000014441 denotes mr-s
T9713 18244-18257 GO_EXT:transcription denotes transcription
T9714 18261-18264 PR_EXT:000005904 denotes Crx
T12279 18287-18291 PR_EXT:000014441 denotes mr-s
T12280 18292-18299 CHEBI_PR_EXT:protein denotes protein
T12281 18304-18311 SO_EXT:0000417 denotes domains
T12282 18337-18344 CHEBI_PR_EXT:protein denotes protein
T12283 18345-18352 CHEBI_PR_EXT:protein denotes protein
T12284 18365-18372 SO_EXT:0000417 denotes modules
T12285 18395-18402 SO_EXT:0000417 denotes domains
T12286 18407-18411 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes bind
T12287 18431-18437 SO_EXT:0000417 denotes domain
T12288 18449-18457 CHEBI_PR_EXT:protein denotes proteins
T12289 18488-18494 SO_EXT:0000417 denotes domain
T12290 18559-18565 SO_EXT:0000417 denotes domain
T12291 18573-18577 PR_EXT:000014441 denotes mr-s
T12292 18578-18585 CHEBI_PR_EXT:protein denotes protein
T12293 18609-18616 CHEBI_PR_EXT:protein denotes protein
T12294 18617-18624 CHEBI_PR_EXT:protein denotes protein
T12295 18637-18643 SO_EXT:0000417 denotes module
T12296 18658-18663 NCBITaxon_EXT:yeast denotes yeast
T12297 18703-18707 PR_EXT:000014441 denotes mr-s
T12298 18708-18715 CHEBI_PR_EXT:protein denotes protein
T12299 18762-18777 GO_EXT:transcription denotes transcriptional
T12300 18788-18794 SO_EXT:sequence_fusion_entity_or_process denotes fusion
T12301 18795-18802 CHEBI_PR_EXT:protein denotes protein
T12302 18820-18825 NCBITaxon:10088 denotes mouse
T12303 18832-18839 UBERON:0000966 denotes retinal
T12304 18840-18845 SO_EXT:cDNA denotes cDNAs
T12305 18851-18856 SO_EXT:sequence_fusion_process denotes fused
T12306 18864-18868 PR_EXT:P04386 denotes GAL4
T12307 18880-18886 SO_EXT:0000417 denotes domain
T12308 18915-18921 SO_EXT:sequence_cloned_entity denotes clones
T12309 18941-18945 SO_EXT:cDNA denotes cDNA
T12310 18975-18981 SO_EXT:0000417 denotes domain
T12311 18985-18989 PR_EXT:000014441 denotes mr-s
T12312 19036-19040 PR_EXT:000014441 denotes mr-s
T12313 19041-19048 CHEBI_PR_EXT:protein denotes protein
T12314 19077-19083 SO_EXT:0000417 denotes domain
T12315 19153-19157 PR_EXT:000014441 denotes mr-s
T12316 19158-19165 CHEBI_PR_EXT:protein denotes protein
T12317 19169-19174 NCBITaxon_EXT:yeast denotes yeast
T12318 19179-19184 SO_EXT:sequence_fusion_process denotes fused
T12319 19200-19209 SO_EXT:sequence_truncation_process denotes truncated
T12320 19226-19230 PR_EXT:000014441 denotes mr-s
T12321 19231-19238 CHEBI_PR_EXT:protein denotes protein
T12322 19246-19249 CHEBI_SO_EXT:DNA denotes DNA
T12323 19250-19257 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T12324 19258-19264 SO_EXT:0000417 denotes domain
T12325 19272-19277 NCBITaxon_EXT:yeast denotes yeast
T12326 19278-19291 GO_EXT:transcription denotes transcription
T12327 19278-19298 GO_EXT:transcription_factor denotes transcription factor
T12328 19299-19303 PR_EXT:P04386 denotes GAL4
T12329 19317-19327 SO_EXT:engineered_biological_sequence denotes constructs
T12330 19332-19337 SO_EXT:sequence_fusion_process denotes fused
T12331 19353-19362 SO_EXT:sequence_truncation_process denotes truncated
T12332 19379-19383 PR_EXT:000014441 denotes mr-s
T12333 19384-19391 CHEBI_PR_EXT:protein denotes protein
T12334 19399-19403 PR_EXT:P04386 denotes GAL4
T12335 19404-19419 GO_EXT:transcription denotes transcriptional
T12336 19431-19437 SO_EXT:0000417 denotes domain
T12337 19451-19461 SO_EXT:engineered_biological_sequence denotes constructs
T12338 19479-19489 SO_EXT:engineered_biological_sequence denotes constructs
T12339 19495-19506 GO:0009294 denotes transformed
T12366 19986-19996 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T12367 20001-20003 CHEBI_SO_EXT:amino_acid denotes aa
T12368 20015-20019 PR_EXT:000014441 denotes mr-s
T12369 20051-20061 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T12370 20066-20068 CHEBI_SO_EXT:amino_acid denotes aa
T12371 20069-20073 PR_EXT:000014441 denotes mr-s
T12372 20074-20081 CHEBI_PR_EXT:protein denotes protein
T12373 20099-20103 PR_EXT:000033987 denotes lacZ
T12374 20104-20117 GO_EXT:transcription denotes transcription
T12375 20132-20142 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T12376 20182-20186 PR_EXT:000033987 denotes lacZ
T12377 20187-20197 GO:0010467 denotes expression
T12378 20218-20228 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminus
T12379 20237-20245 SO_EXT:sequence_coding_function denotes encoding
T12380 20258-20260 CHEBI_SO_EXT:amino_acid denotes aa
T12381 20272-20276 PR_EXT:000014441 denotes mr-s
T12382 20309-20313 PR_EXT:P04386 denotes GAL4
T12383 20367-20371 PR_EXT:000014441 denotes mr-s
T12384 20453-20460 SO_EXT:0000417 denotes domains
T12385 20523-20529 SO_EXT:sequence_fusion_entity_or_process denotes fusion
T12386 20530-20538 CHEBI_PR_EXT:protein denotes proteins
T12387 20552-20557 NCBITaxon_EXT:yeast denotes yeast
T12388 20558-20565 GO:0005634 denotes nucleus
T12389 20577-20581 PR_EXT:P04386 denotes GAL4
T12390 20582-20589 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T12391 20582-20595 SO_EXT:0000409 denotes binding sites
T12392 20620-20626 SO_EXT:0000417 denotes domain
T12393 20685-20692 CHEBI_PR_EXT:protein denotes protein
T12419 22187-22199 GO:0009294 denotes transfection
T12420 22220-22226 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12421 22227-22237 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T12422 22249-22253 PR_EXT:000014441 denotes mr-s
T12423 22306-22312 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12424 22313-22321 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T12425 22322-22329 SO_EXT:sequence_altered_entity denotes mutants
T12426 22338-22349 GO:0009294 denotes transfected
T12427 22395-22398 PR_EXT:000014441 denotes mrs
T12428 23299-23303 PR_EXT:000014441 denotes mr-s
T12429 23304-23311 CHEBI_PR_EXT:protein denotes protein
T12430 23351-23357 SO_EXT:0000417 denotes domain
T12431 23368-23376 GO_EXT:biological_direction_or_guidance denotes directed
T12432 23377-23386 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T12433 23413-23419 SO_EXT:0000417 denotes domain
T12434 23423-23427 PR_EXT:000014441 denotes mr-s
T12435 23453-23462 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T12436 23463-23468 SO_EXT:sequence_alteration_process denotes alter
T12437 23469-23477 CHEBI_EXT:residue denotes residues
T12438 23487-23496 SO_EXT:biological_conservation_process_or_quality denotes conserved
T12439 23508-23514 SO_EXT:0000417 denotes domain
T12440 23562-23571 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T12441 23613-23620 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T12442 23647-23653 SO_EXT:0000417 denotes domain
T12443 23665-23672 CHEBI_PR_EXT:protein denotes protein
T12444 23674-23692 PR_EXT:Q9VHA0 denotes Sex comb on midleg
T12445 23694-23697 PR_EXT:Q9VHA0 denotes Scm
T12446 23759-23767 GO_EXT:biological_direction_or_guidance denotes directed
T12447 23768-23777 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T12448 23803-23812 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T12449 23830-23837 CHEBI_PR_EXT:protein denotes protein
T12450 23849-23855 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12451 23868-23872 PR_EXT:000014441 denotes mr-s
T12452 23927-23934 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T12453 23985-23992 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T12454 24045-24048 PR_EXT:000014441 denotes mrs
T12455 24089-24094 NCBITaxon_EXT:yeast denotes yeast
T12456 24106-24110 PR_EXT:P04386 denotes GAL4
T12457 24136-24140 PR_EXT:000014441 denotes mr-s
T12458 24141-24148 CHEBI_PR_EXT:protein denotes protein
T12459 24190-24196 SO_EXT:0000417 denotes domain
T12460 24220-24230 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T12461 24251-24257 SO_EXT:0000417 denotes domain
T13814 24264-24275 GO_UBERON_EXT:cellular_component_or_cell_part denotes subcellular
T13816 24276-24288 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T13817 24292-24296 PR_EXT:000014441 denotes mr-s
T13818 24297-24304 CHEBI_PR_EXT:protein denotes protein
T13819 24308-24317 NCBITaxon:40674 denotes mammalian
T13820 24318-24323 CL_GO_EXT:cell denotes cells
T13821 24337-24344 GO:0005634 denotes nuclear
T13822 24337-24364 SO_EXT:0001528 denotes nuclear localization signal
T13823 24345-24357 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T13824 24372-24382 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T13825 24390-24394 PR_EXT:000014441 denotes mr-s
T13826 24395-24402 CHEBI_PR_EXT:protein denotes protein
T13827 24443-24447 PR_EXT:000014441 denotes mr-s
T13828 24448-24455 CHEBI_PR_EXT:protein denotes protein
T13829 24456-24465 GO_PATO_EXT:biological_localization_process_or_quality denotes localizes
T13830 24473-24480 GO:0005634 denotes nucleus
T13831 24499-24510 GO_UBERON_EXT:cellular_component_or_cell_part denotes subcellular
T13832 24502-24510 CL_GO_EXT:cell denotes cellular
T13833 24511-24523 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T13834 24527-24531 PR_EXT:000014441 denotes mr-s
T13835 24535-24544 NCBITaxon:40674 denotes mammalian
T13836 24545-24550 CL_GO_EXT:cell denotes cells
T13837 24572-24578 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T13838 24591-24595 PR_EXT:000014441 denotes mr-s
T13839 24596-24603 SO_EXT:0000155 denotes plasmid
T13840 24617-24622 CL_GO_EXT:cell denotes cells
T13841 24660-24664 PR_EXT:000014441 denotes mr-s
T13842 24665-24672 CHEBI_PR_EXT:protein denotes protein
T13843 24673-24682 GO_PATO_EXT:biological_localization_process_or_quality denotes localized
T13844 24697-24707 _FRAGMENT denotes nucleus of
T13845 24716-24721 GO:0005634 denotes cells
T13846 24716-24721 CL_GO_EXT:cell denotes cells
T13847 24759-24771 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T13848 24779-24783 PR_EXT:000014441 denotes mr-s
T13849 24784-24791 CHEBI_PR_EXT:protein denotes protein
T13850 24805-24809 PR_EXT:000014441 denotes mr-s
T13851 24836-24840 CHEBI:51231 denotes DAPI
T13852 24881-24885 PR_EXT:000014441 denotes mr-s
T13853 24886-24893 CHEBI_PR_EXT:protein denotes protein
T13854 24894-24903 GO_PATO_EXT:biological_localization_process_or_quality denotes localizes
T13855 24926-24933 GO:0005634 denotes nucleus
T13856 24937-24946 NCBITaxon:40674 denotes mammalian
T13857 24947-24952 CL_GO_EXT:cell denotes cells
T13858 24983-24987 PR_EXT:000014441 denotes mr-s
T13859 24988-24995 CHEBI_PR_EXT:protein denotes protein
T13860 25011-25026 GO_EXT:transcription denotes transcriptional
T13861 25027-25037 GO:0065007 denotes regulation
T13862 25055-25058 PR_EXT:000007229 denotes TEL
T15900 25362-25366 PR_EXT:P04386 denotes GAL4
T15901 25367-25371 PR_EXT:000014441 denotes mr-s
T15902 25372-25378 SO_EXT:sequence_fusion_entity_or_process denotes fusion
T15903 25379-25386 CHEBI_PR_EXT:protein denotes protein
T15904 25400-25415 GO_EXT:transcription denotes transcriptional
T15905 25442-25450 CHEBI_PR_EXT:protein denotes proteins
T15906 25484-25492 SO_EXT:biological_sequence denotes sequence
T15907 25502-25505 CHEBI_SO_EXT:DNA denotes DNA
T15908 25506-25513 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T15909 25514-25519 SO_EXT:sequence_or_structure_motif denotes motif
T15910 25544-25559 GO_EXT:transcription denotes transcriptional
T15911 25582-25596 MOP:0000629 denotes polymerization
T15912 25601-25608 CHEBI_PR_EXT:protein denotes protein
T15913 25609-25616 CHEBI_PR_EXT:protein denotes protein
T15914 25640-25648 SO_EXT:biological_sequence denotes sequence
T15915 25658-25673 GO_EXT:transcription denotes transcriptional
T15916 25716-25725 GO:0000785 denotes chromatin
T15917 25741-25745 PR_EXT:000014441 denotes mr-s
T15918 25746-25753 CHEBI_PR_EXT:protein denotes protein
T15919 25784-25787 CHEBI_SO_EXT:DNA denotes DNA
T15920 25788-25795 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T15921 25796-25802 SO_EXT:0000417 denotes domain
T15922 25824-25835 CHEBI_EXT:biochemical denotes biochemical
T15923 25848-25852 PR_EXT:000014441 denotes mr-s
T15924 25874-25884 GO_EXT:0045289 denotes luciferase
T15925 25905-25913 CHEBI_EXT:35224 denotes effector
T15926 25914-25922 SO_EXT:0000155 denotes plasmids
T15927 25930-25937 GO:0010467 denotes express
T15928 25946-25954 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T15929 25955-25965 SO_EXT:engineered_biological_sequence denotes constructs
T15930 25969-25973 PR_EXT:000014441 denotes mr-s
T15931 25974-25979 SO_EXT:sequence_fusion_process denotes fused
T15932 25987-25991 PR_EXT:P04386 denotes GAL4
T15933 25992-25995 CHEBI_SO_EXT:DNA denotes DNA
T15934 25996-26003 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T15935 26004-26010 SO_EXT:0000417 denotes domain
T15936 26058-26062 PR_EXT:000014441 denotes mr-s
T15937 26063-26068 SO_EXT:sequence_fusion_process denotes fused
T15938 26072-26076 PR_EXT:P04386 denotes GAL4
T15939 26077-26080 CHEBI_SO_EXT:DNA denotes DNA
T15940 26081-26088 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T15941 26089-26095 SO_EXT:0000417 denotes domain
T15942 26101-26104 PR_EXT:000014441 denotes mrs
T15943 26132-26140 SO_EXT:0000167 denotes promoter
T15944 26141-26148 SO_EXT:0000155 denotes plasmid
T15945 26157-26161 PR_EXT:P04386 denotes GAL4
T15946 26162-26169 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T15947 26162-26175 SO_EXT:0000409 denotes binding sites
T15948 26224-26231 SO_EXT:0000155 denotes plasmid
T15949 26239-26250 GO:0009294 denotes transfected
T15950 26256-26259 PR_EXT:000014441 denotes mrs
T15951 26270-26280 GO_EXT:0045289 denotes luciferase
T15952 26355-26359 PR_EXT:P04386 denotes GAL4
T15953 26360-26363 CHEBI_SO_EXT:DNA denotes DNA
T15954 26364-26371 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T15955 26372-26378 SO_EXT:0000417 denotes domain
T15956 26439-26446 SO_EXT:0000155 denotes plasmid
T15957 26491-26495 PR_EXT:000014441 denotes mr-s
T15958 26504-26508 PR_EXT:P04386 denotes GAL4
T15959 26548-26555 SO_EXT:0000155 denotes plasmid
T15960 26591-26599 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T15961 26600-26610 SO_EXT:engineered_biological_sequence denotes constructs
T15962 26624-26634 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T15963 26639-26641 CHEBI_SO_EXT:amino_acid denotes aa
T15964 26653-26657 PR_EXT:000014441 denotes mr-s
T15965 26659-26670 CHEBI_SO_EXT:amino_acid denotes amino acids
T15966 26688-26698 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminus
T15967 26708-26719 CHEBI_SO_EXT:amino_acid denotes amino acids
T15968 26737-26742 SO_EXT:sequence_fusion_process denotes fused
T15969 26750-26754 PR_EXT:P04386 denotes GAL4
T15970 26773-26774 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N
T15971 26780-26781 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C
T15972 26794-26795 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N
T15973 26852-26853 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C
T15974 26864-26874 GO_EXT:0045289 denotes luciferase
T15975 26875-26879 SO_EXT:0000704 denotes gene
T15976 26875-26890 GO:0010467 denotes gene expression
T15977 26958-26961 PR_EXT:000014441 denotes mrs
T15978 26969-26984 GO_EXT:transcription denotes transcriptional
T15979 27042-27048 SO_EXT:0000417 denotes domain
T15980 27103-27107 PR_EXT:000014441 denotes mr-s
T15981 27124-27139 GO_EXT:transcription denotes transcriptional
T15982 27161-27169 GO_EXT:biological_direction_or_guidance denotes directed
T15983 27170-27177 SO_EXT:sequence_altered_entity denotes mutants
T15984 27236-27243 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T15985 27294-27297 PR_EXT:000014441 denotes mrs
T15986 27398-27402 PR_EXT:000014441 denotes mr-s
T15987 27446-27461 GO_EXT:transcription denotes transcriptional
T15988 27489-27496 SO_EXT:sequence_altered_entity denotes mutants
T15989 27538-27551 GO_EXT:transcription denotes transcription
T15990 27592-27596 PR_EXT:000014441 denotes mr-s
T15991 27609-27624 GO_EXT:transcription denotes transcriptional
T15992 27656-27666 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminus
T15993 27717-27722 SO_EXT:sequence_fusion_process denotes fused
T15994 27730-27734 PR_EXT:P04386 denotes GAL4
T15995 27796-27807 CHEBI_SO_EXT:amino_acid denotes amino acids
T15996 27881-27888 SO_EXT:0000155 denotes plasmid
T15997 27909-27919 GO_EXT:0045289 denotes luciferase
T15998 27965-27976 CHEBI_SO_EXT:amino_acid denotes amino acids
T15999 27996-28007 GO:0009294 denotes transfected
T16000 28027-28034 SO_EXT:0000155 denotes plasmid
T16001 28060-28075 GO_EXT:transcription denotes transcriptional
T16002 28099-28103 PR_EXT:000014441 denotes mr-s
T16003 28107-28112 CL_GO_EXT:cell denotes cells
T16004 28116-28123 UBERON:0000966 denotes retinal
T16005 28171-28176 NCBITaxon:9606 denotes human
T16006 28196-28201 CL_GO_EXT:cell denotes cells
T16007 28234-28237 PR_EXT:000014441 denotes mrs
T16008 28251-28261 GO_EXT:0045289 denotes luciferase
T16009 28307-28312 CL_GO_EXT:cell denotes cells
T16010 28333-28343 GO_EXT:0045289 denotes luciferase
T16011 28387-28392 CL_GO_EXT:cell denotes cells
T16012 28435-28440 CL_GO_EXT:cell denotes cells
T16013 28481-28493 GO:0009294 denotes transfection
T16014 28519-28523 CL_GO_EXT:cell denotes cell
T16015 28559-28572 GO:0005622 denotes intracellular
T16016 28564-28572 CL_GO_EXT:cell denotes cellular
T16017 28592-28597 CL_GO_EXT:cell denotes cells
T16018 28616-28620 CL_GO_EXT:cell denotes cell
T16019 28673-28687 CL:0000210 denotes photoreceptors
T16020 28784-28788 PR_EXT:000014441 denotes mr-s
T16021 28789-28804 GO_EXT:transcription denotes transcriptional
T16022 28883-28888 CL_GO_EXT:cell denotes cells
T16023 28926-28930 PR_EXT:000014441 denotes mr-s
T16024 28946-28961 GO_EXT:transcription denotes transcriptional
T16025 28986-29000 CL:0000210 denotes photoreceptors
T16026 29033-29043 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T16027 29054-29058 PR_EXT:000014441 denotes mr-s
T16028 29060-29071 CHEBI_SO_EXT:amino_acid denotes amino acids
T16029 29100-29115 GO_EXT:transcription denotes transcriptional
T16030 29130-29134 PR_EXT:000014441 denotes mr-s
T16031 29147-29153 SO_EXT:0000417 denotes domain
T16032 29215-29225 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T16033 29236-29241 NCBITaxon:10088 denotes mouse
T16034 29242-29246 PR_EXT:000014441 denotes mr-s
T16035 29257-29266 SO_EXT:biological_conservation_process_or_quality denotes conserved
T16036 29273-29280 NCBITaxon:species denotes species
T16037 29296-29304 SO_EXT:biological_sequence denotes sequence
T16038 29358-29361 NCBITaxon:10114 denotes rat
T16039 29363-29368 NCBITaxon:9606 denotes human
T16040 29370-29375 NCBITaxon_UBERON_EXT:chick denotes chick
T16041 29380-29389 NCBITaxon:7955 denotes zebrafish
T16042 29447-29457 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T16043 29468-29473 NCBITaxon:10088 denotes mouse
T16044 29474-29478 PR_EXT:000014441 denotes mr-s
T16045 29494-29509 GO_EXT:transcription denotes transcriptional
T16046 29521-29527 SO_EXT:0000417 denotes domain
T16047 29531-29544 CL:0000210 denotes photoreceptor
T16048 29531-29556 GO:0042461 denotes photoreceptor development
T20363 31492-31496 SO_EXT:0000704 denotes gene
T20364 31498-31502 PR_EXT:000014441 denotes mr-s
T20365 31527-31536 GO:0010467 denotes expressed
T20366 31540-31547 UBERON:0000966 denotes retinal
T20367 31540-31562 CL:0010009 denotes retinal photoreceptors
T20368 31571-31583 UBERON:0001905 denotes pineal gland
T20369 31597-31601 PR_EXT:000014441 denotes mr-s
T20370 31602-31612 GO:0010467 denotes expression
T20371 31631-31637 UBERON:0000966 denotes retina
T20372 31657-31667 GO:0010467 denotes expression
T20373 31714-31717 PR_EXT:000005904 denotes Crx
T20374 31719-31728 PR_EXT:000001245 denotes rhodopsin
T20375 31739-31752 CL:0000210 denotes photoreceptor
T20376 31753-31758 SO_EXT:0000704 denotes genes
T20377 31788-31809 UBERON:0001790 denotes outer plexiform layer
T20378 31840-31855 UBERON:0001781 denotes layer of retina
T20379 31883-31886 UBERON:0001789 denotes ONL
T20380 31891-31894 UBERON:0001791 denotes INL
T20381 31914-31928 CL:0000210 denotes photoreceptors
T20382 31955-31970 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T20383 31984-31997 GO:0001750 denotes outer segment
T20384 32030-32034 PR_EXT:000014441 denotes mr-s
T20385 32044-32052 CHEBI:36357 denotes molecule
T20386 32065-32094 GO:0042461 denotes development of photoreceptors
T20387 32080-32094 CL:0000210 denotes photoreceptors
T20388 32124-32128 PR_EXT:000012086 denotes Otx2
T20389 32133-32136 PR_EXT:000005904 denotes Crx
T20390 32161-32174 CL:0000210 denotes photoreceptor
T20391 32161-32186 GO:0042461 denotes photoreceptor development
T20392 32196-32200 PR_EXT:000012086 denotes Otx2
T20393 32210-32219 GO:0065007 denotes regulates
T20394 32220-32223 PR_EXT:000005904 denotes Crx
T20395 32224-32237 GO_EXT:transcription denotes transcription
T20396 32253-32266 GO:0097617 denotes hybridization
T20397 32271-32273 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T20398 32310-32314 PR_EXT:000014441 denotes mr-s
T20399 32329-32332 PR_EXT:000005904 denotes Crx
T20400 32336-32342 UBERON:0000966 denotes retina
T20401 32347-32359 UBERON:0001905 denotes pineal gland
T20402 32378-32388 GO_EXT:0045289 denotes luciferase
T20403 32413-32417 PR_EXT:000012086 denotes Otx2
T20404 32422-32425 PR_EXT:000005904 denotes Crx
T20405 32439-32449 GO_EXT:positive_regulation denotes upregulate
T20406 32454-32467 GO_EXT:transcription denotes transcription
T20407 32471-32475 PR_EXT:000014441 denotes mr-s
T20408 32479-32488 NCBITaxon:40674 denotes mammalian
T20409 32489-32494 CL_GO_EXT:cell denotes cells
T20410 32499-32506 UBERON:0000966 denotes retinal
T20411 32499-32526 CL:0010009 denotes retinal photoreceptor cells
T20412 32521-32526 CL_GO_EXT:cell denotes cells
T20413 32532-32536 PR_EXT:000012086 denotes Otx2
T20414 32537-32548 SO_EXT:0000673 denotes transcripts
T20415 32564-32573 GO:0010467 denotes expressed
T20416 32594-32597 PR_EXT:000005904 denotes Crx
T20417 32598-32609 SO_EXT:0000673 denotes transcripts
T20418 32688-32692 PR_EXT:000014441 denotes mr-s
T20419 32693-32706 GO_EXT:transcription denotes transcription
T20420 32719-32728 GO:0065007 denotes regulated
T20421 32732-32735 PR_EXT:000005904 denotes Crx
T20422 32759-32762 PR_EXT:000011432 denotes Nrl
T20423 32766-32779 CL:0000210 denotes photoreceptor
T20424 32789-32802 GO_EXT:transcription denotes transcription
T20425 32789-32809 GO_EXT:transcription_factor denotes transcription factor
T20426 32825-32834 GO:0010467 denotes expressed
T20427 32838-32852 CL:0000210 denotes photoreceptors
T20428 32864-32869 GO:0007567 denotes natal
T20429 32896-32909 GO_EXT:transcription denotes transcription
T20430 32913-32917 PR_EXT:000014441 denotes mr-s
T20431 32980-32983 PR_EXT:000011432 denotes Nrl
T20432 32987-32992 NCBITaxon:10088 denotes mouse
T20433 33031-33041 GO:0010467 denotes expression
T20434 33054-33063 SO_EXT:wild_type_entity_or_quality denotes wild-type
T20435 33068-33071 PR_EXT:000011432 denotes Nrl
T20436 33075-33082 UBERON:0000966 denotes retinas
T20437 33125-33129 PR_EXT:000014441 denotes mr-s
T20438 33169-33178 GO:0010467 denotes expressed
T20439 33179-33184 SO_EXT:0000704 denotes genes
T20440 33192-33195 PR_EXT:000011432 denotes Nrl
T20441 33199-33205 UBERON:0000966 denotes retina
T20442 33247-33253 SO_EXT:0000417 denotes domain
T20443 33259-33266 CHEBI_PR_EXT:protein denotes protein
T20444 33267-33274 CHEBI_PR_EXT:protein denotes protein
T20445 33287-33293 SO_EXT:0000417 denotes module
T20446 33303-33309 SO_EXT:0000417 denotes domain
T20447 33317-33321 PR_EXT:000014441 denotes mr-s
T20448 33322-33329 CHEBI_PR_EXT:protein denotes protein
T20449 33367-33370 PR_EXT:000007229 denotes TEL
T20450 33382-33389 SO_EXT:0000417 denotes domains
T20451 33401-33408 SO_EXT:0001114 denotes helical
T20452 33423-33432 CHEBI:33839 denotes polymeric
T20453 33487-33496 GO:0000785 denotes chromatin
T20454 33528-33539 CHEBI_EXT:biochemical denotes biochemical
T20455 33552-33556 PR_EXT:000014441 denotes mr-s
T20456 33571-33576 NCBITaxon_EXT:yeast denotes yeast
T20457 33616-33620 PR_EXT:000014441 denotes mr-s
T20458 33674-33680 SO_EXT:sequence_cloned_entity denotes clones
T20459 33714-33718 SO_EXT:cDNA denotes cDNA
T20460 33748-33754 SO_EXT:0000417 denotes domain
T20461 33758-33762 PR_EXT:000014441 denotes mr-s
T20462 33792-33796 PR_EXT:000014441 denotes mr-s
T20463 33829-33835 SO_EXT:0000417 denotes domain
T20464 33882-33890 GO_EXT:biological_direction_or_guidance denotes directed
T20465 33891-33898 SO_EXT:sequence_altered_entity denotes mutants
T20466 33910-33916 SO_EXT:0000417 denotes domain
T20467 33920-33924 PR_EXT:000014441 denotes mr-s
T20468 33948-33952 PR_EXT:000014441 denotes mr-s
T20469 33953-33960 CHEBI_PR_EXT:protein denotes protein
T20470 33996-34002 SO_EXT:0000417 denotes domain
T20471 34006-34015 NCBITaxon:40674 denotes mammalian
T20472 34016-34021 CL_GO_EXT:cell denotes cells
T20473 34077-34083 SO_EXT:0000417 denotes domain
T20474 34087-34091 PR_EXT:000014441 denotes mr-s
T20475 34100-34109 CHEBI:33839 denotes polymeric
T20476 34175-34181 SO_EXT:0000417 denotes domain
T20477 34185-34189 PR_EXT:000014441 denotes mr-s
T20478 34204-34210 SO_EXT:0000417 denotes domain
T20479 34222-34231 CHEBI:36357 denotes molecules
T20480 34246-34250 PR_EXT:000014441 denotes mr-s
T20481 34273-34280 CHEBI:33839 denotes polymer
T20482 34293-34297 SO_EXT:0000704 denotes gene
T20483 34293-34307 GO:0016458 denotes gene silencing
T20484 34332-34341 GO:0032991 denotes complexes
T20485 34352-34361 GO:0000785 denotes chromatin
T20486 34383-34386 PR_EXT:000007229 denotes TEL
T20487 34464-34474 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminal
T20488 34475-34485 SO_EXT:engineered_biological_sequence denotes constructs
T20489 34500-34506 SO_EXT:0000417 denotes domain
T20490 34586-34593 CHEBI:33839 denotes polymer
T20491 34654-34661 CHEBI_PR_EXT:protein denotes protein
T20492 34662-34669 CHEBI_PR_EXT:protein denotes protein
T20493 34685-34689 PR_EXT:000014441 denotes mr-s
T20494 34716-34726 GO:0010467 denotes expression
T20495 34767-34771 PR_EXT:000014441 denotes mr-s
T20496 34780-34787 CHEBI:33839 denotes polymer
T20497 34845-34848 PR_EXT:000007229 denotes TEL
T20498 34860-34868 SO_EXT:biological_sequence denotes sequence
T20499 34878-34881 CHEBI_SO_EXT:DNA denotes DNA
T20500 34882-34889 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T20501 34890-34896 SO_EXT:0000417 denotes domain
T20502 34913-34919 SO_EXT:0000417 denotes domain
T20503 34925-34930 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binds
T20504 34961-34967 SO_EXT:0000417 denotes domain
T20505 34974-34977 PR_EXT:000007229 denotes TEL
T20506 35004-35011 CHEBI:33839 denotes polymer
T20507 35035-35050 GO:0051259 denotes oligomerization
T20508 35062-35068 SO_EXT:0000417 denotes domain
T20509 35085-35088 PR_EXT:000007229 denotes TEL
T20510 35121-35129 SO_EXT:biological_sequence denotes sequence
T20511 35139-35142 CHEBI_SO_EXT:DNA denotes DNA
T20512 35143-35150 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T20513 35151-35156 SO_EXT:sequence_or_structure_motif denotes motif
T20514 35186-35193 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T20515 35222-35229 CHEBI_PR_EXT:protein denotes protein
T20516 35230-35237 CHEBI_PR_EXT:protein denotes protein
T20517 35262-35270 SO_EXT:biological_sequence denotes sequence
T20518 35280-35295 GO_EXT:transcription denotes transcriptional
T20519 35325-35329 SO_EXT:0000704 denotes gene
T20520 35330-35338 SO_EXT:sequence_coding_function denotes encoding
T20521 35339-35354 GO_EXT:transcription denotes transcriptional
T20522 35374-35383 PR_EXT:P05084 denotes Hunchback
T20523 35399-35409 GO_EXT:biological_recruitment denotes recruiting
T20524 35414-35420 SO_EXT:0000417 denotes domain
T20525 35436-35444 CHEBI_PR_EXT:protein denotes proteins
T20526 35486-35500 MOP:0000629 denotes polymerization
T20527 35513-35517 PR_EXT:000014441 denotes mr-s
T20528 35543-35546 CHEBI_SO_EXT:DNA denotes DNA
T20529 35547-35554 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T20530 35555-35561 SO_EXT:sequence_or_structure_motif denotes motifs
T20531 35590-35598 SO_EXT:biological_sequence denotes sequence
T20532 35608-35621 GO_EXT:transcription denotes transcription
T20533 35608-35631 GO_EXT:transcription_factor denotes transcription factor(s)
T20534 35653-35657 PR_EXT:000014441 denotes mr-s
T20535 35688-35701 GO_EXT:transcription denotes transcription
T20536 35688-35709 GO_EXT:transcription_factor denotes transcription factors
T20537 35725-35730 NCBITaxon_EXT:yeast denotes yeast
T20538 35784-35788 PR_EXT:000014441 denotes mr-s
T20539 35789-35794 SO_EXT:sequence_fusion_process denotes fused
T20540 35802-35806 PR_EXT:P04386 denotes GAL4
T20541 35807-35810 CHEBI_SO_EXT:DNA denotes DNA
T20542 35811-35818 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T20543 35819-35825 SO_EXT:0000417 denotes domain
T20544 35831-35834 PR_EXT:000014441 denotes mrs
T20545 35851-35866 GO_EXT:transcription denotes transcriptional
T20546 35909-35913 PR_EXT:000014441 denotes mr-s
T20547 35940-35949 GO:0032991 denotes complexes
T20548 35971-35977 SO_EXT:0000417 denotes domain
T20549 35989-35997 CHEBI_PR_EXT:protein denotes proteins
T20550 36057-36061 PR_EXT:000014441 denotes mr-s
T20551 36078-36084 SO_EXT:0000417 denotes domain
T20552 36110-36125 GO_EXT:transcription denotes transcriptional
T20553 36147-36161 MOP:0000629 denotes Polymerization
T20554 36173-36179 SO_EXT:0000417 denotes domain
T20555 36264-36267 PR_EXT:000007229 denotes TEL
T20556 36296-36301 NCBITaxon:9606 denotes human
T20557 36302-36308 GO_EXT:fatality_or_lethality denotes lethal
T20558 36302-36333 PR_EXT:000009640 denotes lethal(3) malignant brain tumor
T20559 36322-36327 UBERON:0000955 denotes brain
T20560 36337-36344 PR_EXT:000009640 denotes L(3)MBT
T20561 36346-36353 CHEBI_PR_EXT:protein denotes protein
T20562 36381-36387 SO_EXT:0000417 denotes domain
T20563 36395-36405 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminus
T20564 36425-36440 GO_EXT:transcription denotes transcriptional
T20565 36481-36482 NCBITaxon:9606 denotes H
T20566 36483-36490 PR_EXT:000009640 denotes L(3)MBT
T20567 36531-36538 SO_EXT:sequence_repeat_unit_or_region denotes repeats
T20568 36555-36561 SO_EXT:0000417 denotes domain
T20569 36585-36600 GO_EXT:transcription denotes transcriptional
T20570 36625-36629 PR_EXT:000014441 denotes mr-s
T20571 36630-36637 CHEBI_PR_EXT:protein denotes protein
T20572 36654-36664 GO_EXT:0045289 denotes luciferase
T20573 36682-36690 GO_EXT:biological_direction_or_guidance denotes directed
T20574 36691-36698 SO_EXT:sequence_altered_entity denotes mutants
T20575 36761-36768 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T20576 36823-36838 GO_EXT:transcription denotes transcriptional
T20577 36862-36866 PR_EXT:000014441 denotes mr-s
T20578 36972-36978 SO_EXT:0000417 denotes domain
T20579 37028-37035 SO_EXT:0000155 denotes plasmid
T20580 37079-37090 CHEBI_SO_EXT:amino_acid denotes amino acids
T20581 37166-37170 PR_EXT:000014441 denotes mr-s
T20582 37172-37197 SO_EXT:biological_conservation_process_or_quality denotes Evolutionary conservation
T20583 37205-37215 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T20584 37219-37221 CHEBI_SO_EXT:amino_acid denotes aa
T20585 37232-37236 PR_EXT:000014441 denotes mr-s
T20586 37242-37251 NCBITaxon:7955 denotes zebrafish
T20587 37260-37265 NCBITaxon:9606 denotes human
T20588 37339-37346 CHEBI_PR_EXT:protein denotes protein
T20589 37347-37360 GO:0031519 denotes PcG complexes
T20590 37362-37366 GO:0035102 denotes PRC1
T20591 37371-37375 GO:0035098 denotes PRC2
T20592 37399-37403 GO:0035098 denotes PRC2
T20593 37437-37446 GO:0016458 denotes silencing
T20594 37460-37467 PR_EXT:000043452 denotes histone
T20595 37460-37467 CHEBI:15358 denotes histone
T20596 37460-37480 GO_EXT:0004407 denotes histone deacetylases
T20597 37482-37487 GO_EXT:0004407 denotes HDACs
T20598 37493-37500 PR_EXT:000043452 denotes histone
T20599 37493-37500 CHEBI:15358 denotes histone
T20600 37493-37519 GO_EXT:0042054 denotes histone methyltransferases
T20601 37531-37540 GO_MOP_EXT:methylation denotes methylate
T20602 37541-37548 CHEBI:15358 denotes histone
T20603 37541-37551 PR_EXT:000027594 denotes histone H3
T20604 37552-37558 CHEBI_SO_EXT:lysine denotes lysine
T20605 37569-37574 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marks
T20606 37578-37586 GO:0016458 denotes silenced
T20607 37578-37596 GO:0035328 denotes silenced chromatin
T20608 37598-37602 GO:0035102 denotes PRC1
T20609 37633-37640 CHEBI:15358 denotes histone
T20610 37633-37643 PR_EXT:000027594 denotes histone H3
T20611 37644-37650 CHEBI_SO_EXT:lysine denotes lysine
T20612 37654-37658 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes mark
T20613 37666-37670 GO:0035098 denotes PRC2
T20614 37703-37707 SO_EXT:0000704 denotes gene
T20615 37728-37732 GO:0035102 denotes PRC1
T20616 37740-37749 GO:0000785 denotes chromatin
T20617 37740-37760 GO:0006338 denotes chromatin remodeling
T20618 37792-37807 GO:0016514 denotes SWI-SNF complex
T20619 37859-37863 GO:0035098 denotes PRC2
T20620 37881-37885 GO_EXT:0004407 denotes HDAC
T20621 37933-37937 GO:0035102 denotes PRC1
T20622 37952-37956 GO_EXT:0004407 denotes HDAC
T20623 37974-37984 GO_EXT:0045289 denotes luciferase
T20624 38008-38023 GO_EXT:transcription denotes transcriptional
T20625 38042-38045 PR_EXT:000014441 denotes mrs
T20626 38108-38122 CHEBI:46024 denotes trichostatin A
T20627 38133-38147 CHEBI_GO_EXT:histone_deacetylase_inhibitor denotes HDAC inhibitor
T20628 38226-38230 PR_EXT:000014441 denotes mr-s
T20629 38238-38242 GO_EXT:0004407 denotes HDAC
T20630 38283-38295 GO:0035102 denotes PRC1 complex
T20631 38323-38334 CHEBI_EXT:biochemical denotes biochemical
T20632 38368-38372 PR_EXT:000014441 denotes mr-s
T20633 38373-38380 CHEBI_PR_EXT:protein denotes protein
T20634 38396-38411 GO_EXT:transcription denotes transcriptional
T20635 38435-38449 GO_EXT:negative_regulation denotes down-regulates
T20636 38475-38485 GO:0010467 denotes expression
T20637 38500-38505 SO_EXT:0000704 denotes genes
T20638 38513-38520 UBERON:0000966 denotes retinal
T20639 38513-38534 CL:0010009 denotes retinal photoreceptor
T20640 38521-38546 GO:0042461 denotes photoreceptor development
T20641 38603-38607 PR_EXT:000014441 denotes mr-s
T20642 38629-38639 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T20643 38648-38659 CHEBI_SO_EXT:amino_acid denotes amino acids
T20644 38704-38708 PR_EXT:000014441 denotes mr-s
T20645 38739-38752 GO:0097617 denotes hybridization
T20646 38777-38781 PR_EXT:000014441 denotes mr-s
T20647 38782-38792 GO:0010467 denotes expression
T20648 38812-38819 UBERON:0000966 denotes retinal
T20649 38812-38834 CL:0010009 denotes retinal photoreceptors
T20650 38852-38867 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T20651 38900-38905 SO_EXT:0000704 denotes genes
T20652 38909-38913 PR_EXT:000014441 denotes mr-s
T20653 38927-38940 CL:0000210 denotes photoreceptor
T20654 38941-38946 SO_EXT:0000704 denotes genes
T20655 38962-38966 PR_EXT:000014441 denotes mr-s
T20656 38984-38994 GO:0010467 denotes expression
T20657 39002-39015 CL:0000210 denotes photoreceptor
T20658 39016-39021 SO_EXT:0000704 denotes genes
T20659 39025-39028 _FRAGMENT denotes rod
T20660 39038-39052 CL:0000604 denotes photoreceptors
T20661 39033-39052 CL:0000573 denotes cone photoreceptors
T20662 39092-39096 PR_EXT:000014441 denotes mr-s
T20663 39112-39116 CL_GO_EXT:cell denotes cell
T20664 39112-39138 _FRAGMENT denotes cell fate determination of
T20665 39143-39157 GO:0043703 denotes photoreceptors
T20666 39165-39184 GO:0042671 denotes cone photoreceptors
T20667 39139-39157 CL:0000604 denotes rod photoreceptors
T20668 39165-39184 CL:0000573 denotes cone photoreceptors
T20669 39192-39211 CL:0000573 denotes cone photoreceptors
T20670 39232-39247 UBERON:0019248 denotes early embryonic
T20671 39238-39254 UBERON_EXT:embryonic_stage_or_stage_part denotes embryonic stages
T20672 39258-39263 NCBITaxon:10088 denotes mouse
T20673 39264-39277 GO:0060041 denotes retinogenesis
T20674 39279-39297 CL:0000604 denotes rod photoreceptors
T20675 39324-39338 _FRAGMENT denotes late embryonic
T20676 39359-39365 UBERON:0007220 denotes period
T22642 39772-39777 NCBITaxon:10088 denotes mouse
T22643 39778-39782 PR_EXT:000014441 denotes mr-s
T22644 39807-39816 GO:0010467 denotes expressed
T22645 39820-39827 UBERON:0000966 denotes retinal
T22646 39820-39842 CL:0010009 denotes retinal photoreceptors
T22647 39851-39863 UBERON:0001905 denotes pineal gland
T22648 39865-39869 PR_EXT:000014441 denotes mr-s
T22649 39873-39897 SO_EXT:biological_conservation_process_or_quality denotes evolutionarily conserved
T22650 39903-39912 NCBITaxon:7955 denotes zebrafish
T22651 39924-39929 NCBITaxon:9606 denotes human
T22652 39964-39968 PR_EXT:000014441 denotes mr-s
T22653 39972-39985 CL:0000210 denotes photoreceptor
T22654 39972-39997 GO:0042461 denotes photoreceptor development
T22655 40029-40033 PR_EXT:000014441 denotes mr-s
T22656 40034-40041 CHEBI_PR_EXT:protein denotes protein
T22657 40042-40051 GO_PATO_EXT:biological_localization_process_or_quality denotes localizes
T22658 40059-40066 GO:0005634 denotes nucleus
T22659 40114-40120 SO_EXT:0000417 denotes domain
T22660 40132-40136 PR_EXT:000014441 denotes mr-s
T22661 40137-40144 CHEBI_PR_EXT:protein denotes protein
T22662 40145-40150 SO_EXT:sequence_fusion_process denotes fused
T22663 40154-40158 PR_EXT:P04386 denotes GAL4
T22664 40178-40193 GO_EXT:transcription denotes transcriptional
T22665 40232-40236 PR_EXT:000014441 denotes mr-s
T22666 40251-40261 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T22667 40270-40280 CHEBI_SO_EXT:amino_acid denotes amino acid
T22668 40310-40314 PR_EXT:000014441 denotes mr-s
T22669 40336-40344 CHEBI:36357 denotes molecule
T22670 40370-40384 _FRAGMENT denotes development of
T22671 40393-40407 GO:0042461 denotes photoreceptors
T22672 40412-40424 GO:0021982 denotes pineal gland
T22673 40385-40392 UBERON:0000966 denotes retinal
T22674 40385-40407 CL:0010009 denotes retinal photoreceptors
T22675 40412-40424 UBERON:0001905 denotes pineal gland
T23178 40449-40454 NCBITaxon:10088 denotes mouse
T23179 40455-40459 PR_EXT:000014441 denotes mr-s
T23180 40460-40464 SO_EXT:cDNA denotes cDNA
T23181 40560-40565 NCBITaxon:10088 denotes mouse
T23182 40566-40571 SO_EXT:0000704 denotes genes
T23183 40572-40581 GO:0010467 denotes expressed
T23184 40604-40610 UBERON:0000966 denotes retina
T23185 40674-40679 NCBITaxon:10088 denotes mouse
T23186 40680-40687 UBERON:0000966 denotes retinal
T23187 40688-40693 SO_EXT:cDNA denotes cDNAs
T23188 40699-40704 SO_EXT:sequence_cloned_entity denotes clone
T23189 40725-40728 NCBITaxon:10090 denotes Mm.
T23190 40748-40756 SO:0000857 denotes homology
T23191 40786-40791 SO_EXT:0000704 denotes genes
T23192 40799-40801 SO_EXT:0000028 denotes bp
T23193 40802-40806 SO_EXT:cDNA denotes cDNA
T23194 40824-40829 SO_EXT:sequence_cloned_entity denotes clone
T23195 40831-40839 SO_EXT:sequence_coding_function denotes encoding
T23196 40840-40851 CHEBI_SO_EXT:amino_acid denotes amino acids
T23197 40877-40879 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T23198 40889-40894 NCBITaxon:10088 denotes mouse
T23199 40898-40905 UBERON:0000966 denotes retinal
T23200 40906-40910 SO_EXT:cDNA denotes cDNA
T23201 40942-40947 CHEBI_SO_EXT:molecular_probe denotes probe
T23202 40979-40992 GO:0097617 denotes hybridization
T23203 41006-41019 GO:0097617 denotes hybridization
T23204 41023-41028 NCBITaxon:10088 denotes mouse
T23205 41035-41042 UBERON:0000966 denotes retinal
T23206 41043-41047 SO_EXT:cDNA denotes cDNA
T23207 41080-41085 NCBITaxon:10088 denotes mouse
T23208 41086-41090 SO_EXT:cDNA denotes cDNA
T23209 41124-41130 SO_EXT:sequence_cloned_entity denotes clones
T23210 41165-41169 PR_EXT:000014441 denotes mr-s
T23211 41183-41191 SO_EXT:sequence_insertion_process denotes inserted
T23212 41225-41228 CHEBI_SO_EXT:DNA denotes DNA
T23213 41306-41316 CHEBI_SO_EXT:nucleotide denotes nucleotide
T23214 41317-41325 SO_EXT:biological_sequence denotes sequence
T23215 41330-41334 PR_EXT:000014441 denotes mr-s
T23216 41335-41339 SO_EXT:0000704 denotes gene
T23500 41434-41447 GO:0097617 denotes hybridization
T23501 41456-41458 SO_EXT:0000028 denotes bp
T23502 41459-41463 SO_EXT:cDNA denotes cDNA
T23503 41476-41480 PR_EXT:000014441 denotes mr-s
T23504 41494-41496 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T23505 41515-41520 CHEBI_SO_EXT:molecular_probe denotes probe
T23506 41533-41546 GO:0097617 denotes hybridization
T23507 41556-41569 GO:0097617 denotes hybridization
T23785 41615-41619 CL_GO_EXT:cell denotes Cell
T23786 41632-41644 GO:0009294 denotes transfection
T23787 41653-41658 CL_GO_EXT:cell denotes cells
T23788 41756-41762 NCBITaxon:27592 denotes bovine
T23789 41763-41768 UBERON:0001977 denotes serum
T23790 41788-41798 CHEBI:17334 denotes penicillin
T23791 41813-41825 CHEBI:17076 denotes streptomycin
T23792 41837-41849 GO:0009294 denotes transfection
T23793 41861-41866 CL_GO_EXT:cell denotes cells
T23794 41889-41906 CHEBI:77635 denotes calcium phosphate
T23795 41925-41937 GO:0009294 denotes transfection
T23796 41938-41945 CHEBI_EXT:33893 denotes reagent
T23797 41974-41979 CL_GO_EXT:cell denotes cells
T23798 42045-42056 CHEBI_EXT:L_glutamine denotes L-glutamine
T23799 42047-42056 CHEBI_SO_EXT:glutamine denotes glutamine
T23800 42085-42103 CHEBI:32139 denotes sodium bicarbonate
T23801 42124-42136 GO:0009294 denotes transfection
T24148 42204-42207 CHEBI_SO_EXT:RNA denotes RNA
T24149 42231-42238 UBERON:0000479 denotes tissues
T24150 42242-42247 UBERON:0007023 denotes adult
T24151 42248-42252 NCBITaxon:10088 denotes mice
T24152 42296-42299 CHEBI_SO_EXT:RNA denotes RNA
T24153 42330-42337 CHEBI:2511 denotes agarose
T24154 42338-42350 CHEBI:16842 denotes formaldehyde
T24155 42359-42370 GO_EXT:biological_movement_or_translocation_process denotes transferred
T24156 42376-42381 CHEBI:25614 denotes nylon
T24157 42425-42427 SO_EXT:0000028 denotes bp
T24158 42428-42432 SO_EXT:cDNA denotes cDNA
T24159 42442-42450 SO_EXT:sequence_coding_function denotes encoding
T24160 42459-42465 SO_EXT:0000417 denotes domain
T24161 42469-42473 PR_EXT:000014441 denotes mr-s
T24162 42488-42493 CHEBI_SO_EXT:molecular_probe denotes probe
T24163 42498-42511 GO:0097617 denotes hybridization
T24164 42513-42526 GO:0097617 denotes Hybridization
T24165 42673-42679 CHEBI:24866 denotes saline
T24166 42680-42687 CHEBI_EXT:citrate denotes citrate
T24167 42693-42715 CHEBI:8984 denotes sodium dodecyl sulfate
T24168 42717-42720 CHEBI:8984 denotes SDS
T24521 42724-42726 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T24522 42746-42749 CHEBI_SO_EXT:RNA denotes RNA
T24523 42773-42779 UBERON:0000479 denotes tissue
T24524 42810-42813 CHEBI_SO_EXT:RNA denotes RNA
T24525 42818-42837 GO:0001171 denotes reverse transcribed
T24526 42872-42874 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T24527 42879-42886 SO_EXT:0000112 denotes primers
T24528 42899-42906 SO_EXT:0000188 denotes introns
T24529 42925-42929 PR_EXT:000014441 denotes mr-s
T24530 42930-42934 SO_EXT:cDNA denotes cDNA
T24531 43034-43036 SO_EXT:0000028 denotes bp
T24532 43097-43103 SO_EXT:0000112 denotes Primer
T24533 43114-43119 NCBITaxon:10088 denotes mouse
T24534 43120-43125 GO_PR_EXT:glyceraldehyde_3_phosphate_dehydrogenase denotes G3PDH
T24535 43212-43214 SO_EXT:0000028 denotes bp
T25083 43283-43288 NCBITaxon_EXT:yeast denotes Yeast
T25084 43314-43318 PR_EXT:P04386 denotes GAL4
T25085 43340-43345 NCBITaxon_EXT:yeast denotes yeast
T25086 43390-43394 PR_EXT:P04386 denotes GAL4
T25087 43470-43476 SO_EXT:sequence_cloning_process denotes cloned
T25088 43493-43497 PR_EXT:000014441 denotes mr-s
T25089 43514-43520 SO_EXT:0000440 denotes vector
T25090 43556-43561 NCBITaxon:10088 denotes mouse
T25091 43568-43575 UBERON:0000966 denotes retinal
T25092 43576-43581 SO_EXT:cDNA denotes cDNAs
T25093 43596-43602 SO_EXT:0000440 denotes vector
T25094 43604-43617 GO_EXT:transfectant_or_tranformant denotes Transformants
T25095 43633-43639 GO_EXT:biological_growth_entity_or_process denotes growth
T25096 43729-43736 SO_EXT:0000155 denotes Plasmid
T25097 43737-43740 CHEBI_SO_EXT:DNA denotes DNA
T25098 43759-43764 NCBITaxon_EXT:yeast denotes yeast
T25099 43775-43780 NCBITaxon_EXT:yeast denotes Yeast
T25100 43781-43788 SO_EXT:0000155 denotes Plasmid
T25101 43839-43846 CHEBI_PR_EXT:protein denotes protein
T25102 43893-43898 GO:0040007 denotes grown
T25103 43948-43955 CHEBI_SO_EXT:leucine denotes leucine
T25104 43957-43967 CHEBI_SO_EXT:tryptophan denotes tryptophan
T25105 43983-43988 CHEBI:75055 denotes X-gal
T25106 44006-44011 CHEBI:75055 denotes X-gal
T25107 44021-44027 SO_EXT:sequence_cloned_entity denotes clones
T25108 44084-44088 PR_EXT:000014441 denotes mr-s
T25109 44120-44130 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T25110 44132-44143 CHEBI_SO_EXT:amino_acid denotes amino acids
T25111 44155-44165 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminus
T25112 44167-44178 CHEBI_SO_EXT:amino_acid denotes amino acids
T25113 44191-44195 PR_EXT:000014441 denotes mr-s
T25114 44219-44226 SO_EXT:0000440 denotes vectors
T25115 44306-44311 CHEBI:75055 denotes X-gal
T26130 44404-44410 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T26131 44411-44415 SO_EXT:cDNA denotes cDNA
T26132 44425-44433 SO_EXT:sequence_coding_function denotes encoding
T26133 44446-44450 PR_EXT:000014441 denotes mr-s
T26134 44469-44472 PR_EXT:000014441 denotes mrs
T26135 44477-44481 SO_EXT:cDNA denotes cDNA
T26136 44491-44499 SO_EXT:sequence_coding_function denotes encoding
T26137 44500-44511 CHEBI_SO_EXT:amino_acid denotes amino acids
T26138 44549-44553 SO_EXT:cDNA denotes cDNA
T26139 44563-44571 SO_EXT:sequence_coding_function denotes encoding
T26140 44572-44583 CHEBI_SO_EXT:amino_acid denotes amino acids
T26141 44647-44657 GO:0010467 denotes expression
T26142 44658-44664 SO_EXT:0000440 denotes vector
T26143 44695-44703 GO_EXT:biological_direction_or_guidance denotes directed
T26144 44704-44711 SO_EXT:sequence_altered_entity denotes mutants
T26145 44765-44774 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T26146 44805-44809 PR_EXT:000014441 denotes mr-s
T26147 44814-44825 GO:0009294 denotes transfected
T26148 44834-44839 CL_GO_EXT:cell denotes cells
T26149 44853-44860 SO_EXT:0000155 denotes plasmid
T26150 44861-44864 CHEBI_SO_EXT:DNA denotes DNA
T26151 44882-44899 CHEBI:77635 denotes calcium phosphate
T26152 44934-44946 GO:0009294 denotes transfection
T26153 44948-44953 CL_GO_EXT:cell denotes cells
T26154 44992-44998 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T26155 45006-45010 CHEBI:9754 denotes Tris
T26156 45011-45014 CHEBI:17883 denotes HCl
T26157 45030-45034 CHEBI_EXT:EDTA denotes EDTA
T26158 45041-45046 CHEBI:6636 denotes MgCl2
T26159 45055-45059 CHEBI:26710 denotes NaCl
T26160 45066-45095 CHEBI:8102 denotes phenylmethylsulfonyl fluoride
T26161 45107-45119 CHEBI:78708 denotes Nonidet P-40
T26162 45125-45133 CHEBI:17754 denotes glycerol
T26163 45154-45172 CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor denotes protease inhibitor
T26164 45173-45181 CHEBI:60004 denotes cocktail
T26165 45226-45230 CL_GO_EXT:cell denotes cell
T26166 45273-45281 GO:0042571 denotes antibody
T26167 45310-45317 CHEBI_PR_EXT:protein denotes protein
T26168 45379-45386 CHEBI_PR_EXT:protein denotes Protein
T26169 45427-45434 CHEBI_PR_EXT:protein denotes protein
T26170 45522-45528 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T26171 45567-45573 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T26172 45581-45585 CHEBI:9754 denotes Tris
T26173 45586-45589 CHEBI:17883 denotes HCl
T26174 45607-45611 CHEBI:26710 denotes NaCl
T26175 45619-45624 CHEBI:6636 denotes MgCl2
T26176 45627-45635 CHEBI_PR_EXT:protein denotes Proteins
T26177 45666-45669 CHEBI:8984 denotes SDS
T26178 45677-45683 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T26179 45688-45691 CHEBI:8984 denotes SDS
T26180 45698-45702 CHEBI:9754 denotes Tris
T26181 45719-45722 CHEBI:18320 denotes DTT
T26182 45727-45735 CHEBI:17754 denotes glycerol
T26183 45743-45753 CHEBI:8682 denotes pyronine Y
T26184 45794-45816 CHEBI:8984 denotes sodium dodecyl sulfate
T26185 45817-45831 CHEBI_EXT:polyacrylamide denotes polyacrylamide
T26186 45853-45856 CHEBI:8984 denotes SDS
T26187 45867-45878 GO_EXT:biological_movement_or_translocation_process denotes transferred
T26188 45886-45900 CHEBI:53325 denotes nitrocellulose
T26189 45922-45930 GO_EXT:biological_movement_or_translocation_process denotes Transfer
T26190 45985-45991 NCBITaxon:9986 denotes rabbit
T26191 46011-46019 GO:0042571 denotes antibody
T26192 46070-46081 NCBITaxon:3704 denotes horseradish
T26193 46082-46092 GO_EXT:0004601 denotes peroxidase
T26194 46094-46104 MOP:0000779 denotes conjugated
T26195 46105-46109 NCBITaxon:9925 denotes goat
T26196 46115-46121 NCBITaxon:9986 denotes rabbit
T26197 46122-46125 GO:0071735 denotes IgG
T26997 46186-46197 GO_UBERON_EXT:cellular_component_or_cell_part denotes Subcellular
T26998 46189-46197 CL_GO_EXT:cell denotes cellular
T26999 46198-46210 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T27000 46223-46234 GO:0009294 denotes transfected
T27001 46239-46246 SO_EXT:0000155 denotes plasmid
T27002 46247-46255 SO_EXT:sequence_coding_function denotes encoding
T27003 46259-46265 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T27004 46278-46282 PR_EXT:000014441 denotes mr-s
T27005 46296-46301 CL_GO_EXT:cell denotes cells
T27006 46310-46315 CL_GO_EXT:cell denotes cells
T27007 46342-46350 CHEBI_PR_EXT:collagen denotes collagen
T27008 46363-46375 GO:0009294 denotes transfection
T27009 46391-46407 CHEBI_EXT:paraformaldehye denotes paraformaldehyde
T27010 46411-46420 CHEBI_EXT:phosphate denotes phosphate
T27011 46421-46429 CHEBI_CHMO_EXT:buffer_process denotes buffered
T27012 46430-46436 CHEBI_EXT:saline_solution denotes saline
T27013 46518-46523 CL_GO_EXT:cell denotes cells
T27014 46547-46559 CHEBI:9750 denotes Triton X-100
T27015 46632-46638 NCBITaxon:27592 denotes bovine
T27016 46639-46644 UBERON:0001977 denotes serum
T27017 46639-46652 PR_EXT:000003918 denotes serum albumin
T27018 46704-46709 CL_GO_EXT:cell denotes cells
T27019 46723-46731 GO:0042571 denotes antibody
T27020 46814-46819 CL_GO_EXT:cell denotes cells
T27021 46862-46865 CHEBI:37987 denotes Cy3
T27022 46866-46876 MOP:0000779 denotes conjugated
T27023 46877-46881 NCBITaxon:9925 denotes goat
T27024 46882-46885 GO:0071735 denotes IgG
T27025 46894-46900 NCBITaxon:9986 denotes rabbit
T27026 46901-46904 GO:0071735 denotes IgG
T27027 46978-46983 CL_GO_EXT:cell denotes cells
T27828 47763-47769 SO_EXT:0000440 denotes vector
T27829 47111-47121 GO_EXT:0045289 denotes Luciferase
T27830 47132-47147 GO_EXT:transcription denotes transcriptional
T27831 47169-47170 CHEBI_SO_EXT:base denotes b
T27832 47171-47179 SO_EXT:0000167 denotes promoter
T27833 47190-47194 PR_EXT:000014441 denotes mr-s
T27834 47222-47229 SO_EXT:0000155 denotes plasmid
T27835 47261-47262 CHEBI_SO_EXT:base denotes b
T27836 47263-47271 SO_EXT:sequence_upstreamness denotes upstream
T27837 47272-47279 SO_EXT:0001026 denotes genomic
T27838 47280-47291 _FRAGMENT denotes fragment of
T27839 47297-47301 SO_EXT:0000997 denotes gene
T27840 47292-47296 PR_EXT:000014441 denotes mr-s
T27841 47314-47324 GO_EXT:0045289 denotes luciferase
T27842 47334-47341 SO_EXT:0000155 denotes plasmid
T27843 47357-47367 GO:0010467 denotes expression
T27844 47368-47375 SO_EXT:0000440 denotes vectors
T27845 47419-47422 PR_EXT:000005904 denotes Crx
T27846 47424-47428 PR_EXT:000012086 denotes Otx2
T27847 47430-47433 PR_EXT:000011432 denotes Nrl
T27848 47434-47439 SO_EXT:0000704 denotes genes
T27849 47452-47462 GO:0010467 denotes expression
T27850 47463-47469 SO_EXT:0000440 denotes vector
T27851 47533-47539 SO_EXT:0000440 denotes vector
T27852 47545-47548 PR_EXT:000005904 denotes Crx
T27853 47549-47556 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T27854 47549-47561 SO_EXT:0000409 denotes binding site
T27855 47575-47577 SO_EXT:0000028 denotes bp
T27856 47591-47598 SO_EXT:sequence_alteration_process denotes mutated
T27857 47602-47611 SO_EXT:sequence_substitution_process denotes replacing
T27858 47649-47655 SO_EXT:0000440 denotes vector
T27859 47661-47664 PR_EXT:000005904 denotes Crx
T27860 47665-47672 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T27861 47665-47677 SO_EXT:0000409 denotes binding site
T27862 47690-47692 SO_EXT:0000028 denotes bp
T27863 47706-47713 SO_EXT:sequence_alteration_process denotes mutated
T27864 47717-47726 SO_EXT:sequence_substitution_process denotes replacing
T27865 47775-47778 PR_EXT:000005904 denotes Crx
T27866 47779-47786 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T27867 47779-47791 SO_EXT:0000409 denotes binding site
T27868 47803-47805 SO_EXT:0000028 denotes bp
T27869 47819-47826 SO_EXT:sequence_alteration_process denotes mutated
T27870 47830-47839 SO_EXT:sequence_substitution_process denotes replacing
T27871 47869-47872 SO_EXT:sequence_altered_entity_or_alteration_process denotes mut
T27872 47878-47884 SO_EXT:0000440 denotes vector
T27873 47899-47902 PR_EXT:000005904 denotes Crx
T27874 47903-47910 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T27875 47903-47916 SO_EXT:0000409 denotes binding sites
T27876 47922-47929 SO_EXT:sequence_alteration_process denotes mutated
T27877 47960-47967 SO_EXT:0000155 denotes plasmid
T27878 47989-47995 SO_EXT:sequence_copy_entity denotes copies
T27879 48003-48007 PR_EXT:P04386 denotes GAL4
T27880 48008-48011 CHEBI_SO_EXT:DNA denotes DNA
T27881 48024-48032 SO_EXT:biological_sequence denotes sequence
T27882 48056-48064 SO_EXT:sequence_upstreamness denotes upstream
T27883 48068-48072 NCBITaxon:10633 denotes SV40
T27884 48081-48089 SO_EXT:0000167 denotes promoter
T27885 48158-48173 GO_EXT:transcription denotes transcriptional
T27886 48186-48190 PR_EXT:000014441 denotes mr-s
T27887 48209-48217 CHEBI_EXT:35224 denotes effector
T27888 48218-48226 SO_EXT:0000155 denotes plasmids
T27889 48236-48244 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T27890 48311-48317 SO_EXT:0000440 denotes vector
T27891 48372-48380 SO_EXT:biological_sequence denotes sequence
T27892 48388-48395 SO_EXT:sequence_coding_function denotes encodes
T27893 48396-48400 PR_EXT:P04386 denotes GAL4
T27894 48401-48404 CHEBI_SO_EXT:DNA denotes DNA
T27895 48405-48412 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T27896 48413-48419 SO_EXT:0000417 denotes domain
T27897 48425-48433 SO_EXT:sequence_insertion_process denotes inserted
T27898 48450-48461 GO:0009294 denotes transfected
T27899 48481-48488 SO_EXT:0000155 denotes plasmid
T27900 48489-48492 CHEBI_SO_EXT:DNA denotes DNA
T27901 48509-48519 GO:0010467 denotes expression
T27902 48520-48526 SO_EXT:0000440 denotes vector
T27903 48527-48530 CHEBI_SO_EXT:DNA denotes DNA
T27904 48558-48563 CL_GO_EXT:cell denotes cells
T27905 48578-48590 GO:0009294 denotes transfection
T27906 48591-48598 CHEBI_EXT:33893 denotes reagent
T27907 48612-48622 GO_EXT:0045289 denotes luciferase
T27908 48644-48656 GO:0009294 denotes transfection
T28655 9160-9164 PR_EXT:000014441 denotes mr-s
T28656 9165-9175 CHEBI_SO_EXT:nucleotide denotes nucleotide
T28657 9180-9190 CHEBI_SO_EXT:amino_acid denotes amino acid
T28658 9191-9200 SO_EXT:biological_sequence denotes sequences
T28659 9206-9210 PR_EXT:000014441 denotes mr-s
T28660 9211-9221 CHEBI_SO_EXT:nucleotide denotes nucleotide
T28661 9226-9237 CHEBI_SO_EXT:amino_acid denotes amino acids
T28662 9238-9247 SO_EXT:biological_sequence denotes sequences
T28663 9255-9266 CHEBI_SO_EXT:amino_acid denotes amino acids
T28664 9279-9285 SO_EXT:0000417 denotes domain
T28665 9286-9294 SO_EXT:biological_sequence denotes sequence
T28666 9335-9342 GO:0005634 denotes nuclear
T28667 9335-9362 SO_EXT:0001528 denotes nuclear localization signal
T28668 9343-9355 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T28669 9399-9414 GO:0043631 denotes polyadenylation
T28670 9399-9433 SO_EXT:0000551 denotes polyadenylation termination signal
T28671 9456-9462 SO_EXT:0000417 denotes domain
T28672 9463-9472 SO_EXT:biological_sequence denotes sequences
T28673 9481-9487 SO_EXT:0000417 denotes domain
T28674 9499-9507 CHEBI_PR_EXT:protein denotes proteins
T28675 9518-9531 SO_EXT:0001117 denotes alpha helices
T28676 9550-9559 SO_EXT:biological_conservation_process_or_quality denotes Conserved
T28677 9560-9570 CHEBI_SO_EXT:amino_acid denotes amino acid
T28678 9571-9579 CHEBI_EXT:residue denotes residues
T28679 9634-9642 CHEBI_EXT:residue denotes residues
T28680 9707-9718 SO_EXT:sequence_alteration_process denotes mutagenesis
T28681 9773-9779 SO_EXT:0000417 denotes domain
T28682 9791-9799 CHEBI_PR_EXT:protein denotes proteins
T28683 9801-9811 CHEBI_SO_EXT:amino_acid denotes Amino acid
T28684 9812-9821 SO_EXT:biological_sequence denotes sequences
T28685 9927-9940 SO_EXT:sequence_substitution_entity_or_process denotes substitutions
T28686 9983-9993 CHEBI_SO_EXT:amino_acid denotes amino acid
T28687 9994-10003 SO_EXT:biological_sequence denotes sequences
T28688 10008-10013 NCBITaxon:10088 denotes mouse
T28689 10015-10018 NCBITaxon:10114 denotes rat
T28690 10020-10025 NCBITaxon:9606 denotes human
T28691 10027-10032 NCBITaxon_UBERON_EXT:chick denotes chick
T28692 10037-10046 NCBITaxon:7955 denotes zebrafish
T28693 10047-10051 PR_EXT:000014441 denotes mr-s
T28694 10052-10060 CHEBI_PR_EXT:protein denotes proteins
T28695 10096-10103 SO_EXT:0000417 denotes domains
T28696 10158-10163 NCBITaxon:10088 denotes mouse
T28697 10164-10171 CHEBI_PR_EXT:protein denotes protein
T28698 10190-10198 SO_EXT:biological_sequence denotes sequence
T28699 10219-10224 NCBITaxon:10088 denotes mouse
T28700 10225-10232 CHEBI_PR_EXT:protein denotes protein
T29398 11423-11427 PR_EXT:000014441 denotes mr-s
T29399 11428-11438 GO:0010467 denotes expression
T29400 11453-11458 NCBITaxon:10088 denotes mouse
T29401 11459-11465 UBERON:0000966 denotes retina
T29402 11470-11482 UBERON:0001905 denotes pineal gland
T29403 11490-11494 PR_EXT:000014441 denotes mr-s
T29404 11495-11505 GO:0010467 denotes expression
T29405 11513-11527 _FRAGMENT denotes development of
T29406 11538-11544 GO:0060041 denotes retina
T29407 11532-11537 NCBITaxon:10088 denotes mouse
T29408 11538-11544 UBERON:0000966 denotes retina
T29409 11558-11571 GO:0097617 denotes hybridization
T29410 11582-11586 PR_EXT:000014441 denotes mr-s
T29411 11702-11706 PR_EXT:000014441 denotes mr-s
T29412 11736-11744 _FRAGMENT denotes layer of
T29413 11749-11755 UBERON:0001781 denotes retina
T29414 11803-11808 UBERON:0007023 denotes adult
T29415 11809-11815 UBERON:0000966 denotes retina
T29416 11846-11851 CHEBI_SO_EXT:molecular_probe denotes probe
T29417 11859-11865 UBERON:0000966 denotes retina
T29418 11929-11933 PR_EXT:000014441 denotes mr-s
T29419 11934-11944 GO:0010467 denotes expression
T29420 11948-11953 UBERON:0007023 denotes adult
T29421 11954-11959 NCBITaxon:10088 denotes mouse
T29422 11960-11966 UBERON:0000062 denotes organs
T29423 11999-12003 PR_EXT:000014441 denotes mr-s
T29424 12004-12014 SO_EXT:0000673 denotes transcript
T29425 12020-12022 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T29426 12045-12049 CHEBI_SO_EXT:RNA denotes RNAs
T29427 12075-12081 UBERON:0000922 denotes embryo
T29428 12086-12096 UBERON:0000468 denotes whole body
T29429 12113-12116 UBERON:0000970 denotes eye
T29430 12122-12128 UBERON:0000966 denotes retina
T29431 12133-12145 UBERON:0001905 denotes pineal gland
T29432 12150-12155 UBERON:0000955 denotes brain
T29433 12160-12165 UBERON:0002107 denotes liver
T29434 12167-12172 UBERON:0007023 denotes adult
T29435 12173-12179 UBERON:0000966 denotes retina
T29436 12181-12186 UBERON:0007023 denotes adult
T29437 12187-12199 UBERON:0001905 denotes pineal gland
T29438 12201-12206 UBERON:0007023 denotes adult
T29439 12207-12212 UBERON:0000955 denotes brain
T29440 12217-12222 UBERON:0007023 denotes adult
T29441 12223-12228 UBERON:0002107 denotes liver
T29442 12244-12247 UBERON:0001782 denotes RPE
T29443 12249-12275 UBERON:0001782 denotes retinal pigment epithelium
T29444 12257-12264 CHEBI_EXT:26130 denotes pigment
T29445 12277-12279 UBERON:0003902 denotes NR
T29446 12281-12294 UBERON:0003902 denotes neural retina
T29447 12301-12313 CL:0000031 denotes neuroblastic
T29448 12321-12324 UBERON:0001792 denotes GCL
T29449 12326-12339 CL:0000740 denotes ganglion cell
T29450 12326-12345 UBERON:0001792 denotes ganglion cell layer
T29451 12335-12339 CL_GO_EXT:cell denotes cell
T29452 12347-12350 UBERON:0001789 denotes ONL
T29453 12352-12371 UBERON:0001789 denotes outer nuclear layer
T29454 12358-12365 GO:0005634 denotes nuclear
T29455 12373-12376 UBERON:0001791 denotes INL
T29456 12378-12397 UBERON:0001791 denotes inner nuclear layer
T29457 12384-12391 GO:0005634 denotes nuclear
T30199 15002-15015 GO_EXT:transcription denotes transcription
T30200 15019-15023 PR_EXT:000014441 denotes mr-s
T30201 15027-15036 GO:0065007 denotes regulated
T30202 15040-15043 PR_EXT:000005904 denotes Crx
T30203 15060-15073 GO:0097617 denotes hybridization
T30204 15082-15087 CHEBI_SO_EXT:molecular_probe denotes probe
T30205 15092-15097 NCBITaxon:10088 denotes mouse
T30206 15098-15102 PR_EXT:000014441 denotes mr-s
T30207 15124-15133 SO_EXT:wild_type_entity_or_quality denotes wild-type
T30208 15142-15145 PR_EXT:000005904 denotes Crx
T30209 15149-15156 UBERON:0000966 denotes retinas
T30210 15168-15172 PR_EXT:000014441 denotes mr-s
T30211 15173-15183 GO:0010467 denotes expression
T30212 15215-15218 PR_EXT:000005904 denotes Crx
T30213 15222-15228 UBERON:0000966 denotes retina
T30214 15257-15259 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T30215 15282-15286 CHEBI_SO_EXT:RNA denotes RNAs
T30216 15306-15319 UBERON:0001905 denotes pineal glands
T30217 15326-15335 SO_EXT:wild_type_entity_or_quality denotes wild-type
T30218 15340-15343 PR_EXT:000005904 denotes Crx
T30219 15347-15352 NCBITaxon:10088 denotes mouse
T30220 15385-15397 SO_EXT:0000006 denotes PCR products
T30221 15415-15421 SO_EXT:0000112 denotes primer
T30222 15441-15445 PR_EXT:000014441 denotes mr-s
T30223 15450-15455 GO_PR_EXT:glyceraldehyde_3_phosphate_dehydrogenase denotes G3PDH
T30224 15456-15461 SO_EXT:cDNA denotes cDNAs
T30225 15477-15482 CHEBI:15377 denotes Water
T30226 15504-15506 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T30227 15527-15530 PR_EXT:000005904 denotes Crx
T30228 15546-15550 PR_EXT:000014441 denotes mr-s
T30229 15551-15564 GO_EXT:transcription denotes transcription
T30230 15575-15583 SO_EXT:0000155 denotes plasmids
T30231 15592-15602 GO_EXT:0045289 denotes luciferase
T30232 15641-15644 PR_EXT:000005904 denotes Crx
T30233 15645-15652 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T30234 15645-15658 SO_EXT:0000409 denotes binding sites
T30235 15689-15699 GO_EXT:0045289 denotes luciferase
T30236 15733-15744 GO:0009294 denotes transfected
T30237 15750-15753 PR_EXT:000005904 denotes Crx
T30238 15755-15759 PR_EXT:000012086 denotes Otx2
T30239 15761-15764 PR_EXT:000011432 denotes Nrl
T30240 15770-15773 PR_EXT:000005904 denotes Crx
T30241 15774-15777 PR_EXT:000011432 denotes Nrl
T30242 15867-15870 SO_EXT:sequence_altered_entity_or_alteration_process denotes mut
T30243 15883-15894 GO:0009294 denotes transfected
T30244 15904-15907 PR_EXT:000005904 denotes Crx
T30245 15908-15918 GO:0010467 denotes expression
T30246 15919-15925 SO_EXT:0000440 denotes vector
T30247 15927-15930 PR_EXT:000005904 denotes Crx
T30248 15946-15952 SO_EXT:0000440 denotes vector
T30249 15954-15957 PR_EXT:000005904 denotes Crx
T30250 15973-15978 CL_GO_EXT:cell denotes cells
T30864 20751-20756 NCBITaxon_EXT:yeast denotes yeast
T30865 20782-20786 PR_EXT:P04386 denotes GAL4
T30866 20810-20814 PR_EXT:000014441 denotes mr-s
T30867 20837-20842 NCBITaxon_EXT:yeast denotes yeast
T30868 20877-20883 SO_EXT:sequence_cloned_entity denotes clones
T30869 20921-20925 PR_EXT:000014441 denotes mr-s
T30870 20926-20932 SO_EXT:sequence_cloned_entity denotes clones
T30871 20973-20980 SO_EXT:0000417 denotes domains
T30872 21011-21015 PR_EXT:000014441 denotes mr-s
T30873 21016-21022 SO_EXT:sequence_fusion_entity_or_process denotes fusion
T30874 21023-21031 CHEBI_PR_EXT:protein denotes proteins
T30875 21044-21049 NCBITaxon_EXT:yeast denotes yeast
T30876 21050-21054 PR_EXT:P04386 denotes GAL4
T30877 21104-21111 SO_EXT:0000417 denotes domains
T30878 21132-21136 PR_EXT:000033987 denotes LacZ
T30879 21137-21147 GO:0010467 denotes expression
T30880 21334-21341 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T30881 21342-21348 SO_EXT:0000417 denotes domain
T30882 21365-21371 SO_EXT:0000417 denotes domain
T30883 21391-21395 PR_EXT:000014441 denotes mr-s
T30884 21397-21398 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N
T30885 21400-21410 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminal
T30886 21422-21426 PR_EXT:000014441 denotes mr-s
T30887 21428-21439 CHEBI_SO_EXT:amino_acid denotes amino acids
T30888 21451-21452 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C
T30889 21454-21464 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T30890 21476-21480 PR_EXT:000014441 denotes mr-s
T30891 21482-21493 CHEBI_SO_EXT:amino_acid denotes amino acids
T31382 22535-22539 PR_EXT:000014441 denotes mr-s
T31383 22540-22547 CHEBI_PR_EXT:protein denotes protein
T31384 22570-22579 NCBITaxon:40674 denotes mammalian
T31385 22580-22585 CL_GO_EXT:cell denotes cells
T31386 22616-22626 SO_EXT:engineered_biological_sequence denotes constructs
T31387 22666-22672 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T31388 22681-22687 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T31389 22701-22712 CHEBI_SO_EXT:amino_acid denotes amino acids
T31390 22730-22741 CHEBI_SO_EXT:amino_acid denotes amino acids
T31391 22761-22772 CHEBI_SO_EXT:amino_acid denotes amino acids
T31392 22798-22806 SO_EXT:sequence_insertion_process denotes inserted
T31393 22819-22825 SO_EXT:0000440 denotes vector
T31394 22849-22859 SO_EXT:engineered_biological_sequence denotes constructs
T31395 22881-22892 GO:0009294 denotes transfected
T31396 22906-22911 CL_GO_EXT:cell denotes cells
T31397 22962-22970 GO:0042571 denotes antibody
T31398 22995-23003 GO:0042571 denotes antibody
T31399 23011-23018 CHEBI_PR_EXT:protein denotes protein
T31400 23067-23073 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T31401 23083-23091 GO_EXT:biological_direction_or_guidance denotes directed
T31402 23092-23096 PR_EXT:000014441 denotes mr-s
T31403 23097-23104 SO_EXT:sequence_altered_entity denotes mutants
T31404 23154-23165 GO:0009294 denotes transfected
T31405 23227-23235 GO:0042571 denotes antibody
T31406 23262-23270 GO:0042571 denotes antibody
T31716 25070-25081 GO_UBERON_EXT:cellular_component_or_cell_part denotes Subcellular
T31717 25073-25081 CL_GO_EXT:cell denotes cellular
T31718 25082-25094 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T31719 25098-25102 PR_EXT:000014441 denotes mr-s
T31720 25106-25115 NCBITaxon:40674 denotes mammalian
T31721 25116-25121 CL_GO_EXT:cell denotes cells
T31722 25133-25139 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T31723 25152-25156 PR_EXT:000014441 denotes mr-s
T31724 25161-25172 GO:0009294 denotes transfected
T31725 25214-25222 GO:0042571 denotes antibody
T31726 25260-25265 CL_GO_EXT:cell denotes cells
T31727 25293-25301 GO:0042571 denotes antibody
T31728 25307-25311 CHEBI:51231 denotes DAPI
T32395 29763-29767 PR_EXT:000014441 denotes mr-s
T32396 29768-29773 SO_EXT:sequence_fusion_process denotes fused
T32397 29777-29781 PR_EXT:P04386 denotes GAL4
T32398 29782-29785 CHEBI_SO_EXT:DNA denotes DNA
T32399 29786-29793 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T32400 29794-29800 SO_EXT:0000417 denotes domain
T32401 29816-29831 GO_EXT:transcription denotes transcriptional
T32402 29853-29858 CL_GO_EXT:cell denotes cells
T32403 29889-29899 SO_EXT:engineered_biological_sequence denotes constructs
T32404 29913-29923 GO_EXT:0045289 denotes luciferase
T32405 29952-29959 SO_EXT:0000155 denotes plasmid
T32406 29967-29978 GO:0009294 denotes transfected
T32407 29992-29997 CL_GO_EXT:cell denotes cells
T32408 30003-30011 CHEBI_EXT:35224 denotes effector
T32409 30012-30020 SO_EXT:0000155 denotes plasmids
T32410 30021-30031 GO:0010467 denotes expressing
T32411 30040-30048 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T32412 30049-30056 SO_EXT:sequence_altered_entity denotes mutants
T32413 30057-30062 SO_EXT:sequence_fusion_process denotes fused
T32414 30066-30070 PR_EXT:P04386 denotes GAL4
T32415 30108-30111 PR_EXT:000014441 denotes mrs
T32416 30117-30118 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N
T32417 30126-30127 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C
T32418 30128-30136 SO_EXT:0000155 denotes plasmids
T32419 30142-30153 GO:0009294 denotes transfected
T32420 30190-30197 SO_EXT:0000155 denotes plasmid
T32421 30251-30257 SO_EXT:0000440 denotes vector
T32422 30369-30380 GO:0009294 denotes transfected
T32423 30394-30399 CL_GO_EXT:cell denotes cells
T32424 30426-30433 SO_EXT:0000155 denotes plasmid
T32425 30490-30500 GO_EXT:0045289 denotes luciferase
T32426 30528-30531 PR_EXT:000014441 denotes mrs
T32427 30622-30633 GO:0009294 denotes transfected
T32428 30660-30667 SO_EXT:0000155 denotes plasmid
T32429 30732-30735 PR_EXT:000014441 denotes mrs
T32430 30750-30761 GO:0009294 denotes transfected
T32431 30786-30791 CL_GO_EXT:cell denotes cells
T32432 30828-30835 SO_EXT:0000155 denotes plasmid
T32433 31050-31060 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T32434 31073-31078 NCBITaxon:10088 denotes mouse
T32435 31080-31083 NCBITaxon:10114 denotes rat
T32436 31085-31090 NCBITaxon:9606 denotes human
T32437 31092-31097 NCBITaxon_UBERON_EXT:chick denotes chick
T32438 31102-31111 NCBITaxon:7955 denotes zebrafish
T32439 31112-31116 PR_EXT:000014441 denotes mr-s
T32440 31117-31125 CHEBI_PR_EXT:protein denotes proteins
T32441 31127-31136 SO_EXT:biological_conservation_process_or_quality denotes Conserved
T32442 31137-31147 CHEBI_SO_EXT:amino_acid denotes amino acid
T32443 31148-31156 CHEBI_EXT:residue denotes residues
T16054 31314-31318 PR_EXT:000014441 denotes mr-s
T16055 31374-31380 SO_EXT:0000417 denotes domain
T20679 39398-39402 PR_EXT:000014441 denotes mr-s
T20680 39420-39424 PR_EXT:000014441 denotes mr-s
T20681 39428-39437 GO:0010467 denotes expressed
T20682 39441-39459 CL:0000604 denotes rod photoreceptors
T20683 39471-39490 CL:0000573 denotes cone photoreceptors
T20684 39502-39507 PR_EXT:000011403 denotes Nr2e3
T13815 24267-24275 CL_GO_EXT:cell denotes cellular
T7264 10890-10900 SO_EXT:0000673 denotes transcript
T7265 10940-10953 CL:0000210 denotes photoreceptor
T7266 10940-10959 UBERON:0001787 denotes photoreceptor layer
T7267 10978-10982 PR_EXT:000014441 denotes mr-s
T7268 10995-11005 GO:0010467 denotes expression
T7269 11013-11026 CL:0000210 denotes photoreceptor
T7270 11013-11032 UBERON:0001787 denotes photoreceptor layer
T7271 11095-11100 CL_GO_EXT:cell denotes cells
T7272 11101-11111 GO:0010467 denotes expressing
T7273 11112-11121 PR_EXT:000001245 denotes rhodopsin
T7274 11132-11149 GO:0007602 denotes phototransduction
T7275 11150-11155 SO_EXT:0000704 denotes genes
T7276 11207-11211 PR_EXT:000014441 denotes mr-s
T7277 11253-11262 GO_PATO_EXT:biological_localization_process_or_quality denotes localized
T7278 11270-11289 UBERON:0001789 denotes outer nuclear layer
T7279 11276-11283 GO:0005634 denotes nuclear
T7280 11291-11294 UBERON:0001789 denotes ONL
T7281 11320-11330 GO:0010467 denotes expression
T7282 11334-11338 PR_EXT:000014441 denotes mr-s
T7283 11339-11343 CHEBI_SO_EXT:mRNA denotes mRNA
T7284 11367-11381 CL:0000210 denotes photoreceptors
T7285 11389-11394 UBERON:0007023 denotes adult
T7286 11395-11401 UBERON:0000966 denotes retina
T7287 12400-12401 GO:0097617 denotes o
T7288 12407-12408 PR_EXT:000014441 denotes m
T7289 12416-12418 UBERON:0001781 denotes ti
T7290 12420-12421 UBERON:0007023 denotes u
T7291 12421-12422 UBERON:0000966 denotes e
T7292 12424-12426 CHEBI_SO_EXT:molecular_probe denotes pe
T7293 12429-12430 GO:0010467 denotes i
T7294 12431-12433 SO_EXT:0000673 denotes it
T7295 12439-12440 UBERON:0000922 denotes r
T7296 12440-12442 PR_EXT:000014441 denotes -s
T7297 12443-12453 GO:0010467 denotes expression
T7298 12459-12469 GO:0010467 denotes expression
T7299 12477-12481 PR_EXT:000014441 denotes mr-s
T7300 12482-12486 SO_EXT:0000704 denotes gene
T9556 14214-14218 PR_EXT:000014441 denotes mr-s
T9557 14226-14235 GO:0065007 denotes regulated
T9558 14239-14242 PR_EXT:000005904 denotes Crx
T9559 14260-14263 PR_EXT:000005904 denotes Crx
T9560 14264-14273 GO:0065007 denotes regulates
T9561 14274-14278 PR_EXT:000014441 denotes mr-s
T9562 14279-14292 GO_EXT:transcription denotes transcription
T9563 14315-14328 GO:0097617 denotes hybridization
T9564 14332-14336 PR_EXT:000014441 denotes mr-s
T9565 14337-14341 CHEBI_SO_EXT:mRNA denotes mRNA
T9566 14345-14354 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9567 14359-14362 PR_EXT:000005904 denotes Crx
T9568 14369-14376 UBERON:0000966 denotes retinas
T9569 14398-14401 PR_EXT:000005904 denotes Crx
T9570 14405-14411 UBERON:0000966 denotes retina
T9571 14417-14421 PR_EXT:000014441 denotes mr-s
T9572 14422-14432 SO_EXT:0000673 denotes transcript
T9573 14489-14492 PR_EXT:000005904 denotes Crx
T9574 14529-14533 PR_EXT:000014441 denotes mr-s
T9575 14565-14578 GO_EXT:transcription denotes transcription
T9576 14582-14586 PR_EXT:000014441 denotes mr-s
T9577 14595-14604 GO:0065007 denotes regulated
T9578 14608-14611 PR_EXT:000005904 denotes Crx
T9579 14619-14631 UBERON:0001905 denotes pineal gland
T9580 14633-14635 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T9581 14669-14684 GO_EXT:transcription denotes transcriptional
T9615 16073-16074 GO:0009294 denotes s
T9616 16076-16077 PR_EXT:000005904 denotes r
T9617 16080-16088 GO_EXT:transcription denotes cription
T9618 16126-16136 GO_EXT:0045289 denotes luciferase
T9619 16158-16159 CHEBI_SO_EXT:base denotes b
T9620 16160-16184 SO_EXT:0001668 denotes proximal promoter region
T9621 16188-16192 PR_EXT:000014441 denotes mr-s
T9622 16193-16198 SO_EXT:sequence_fusion_process denotes fused
T9623 16204-16214 GO_EXT:0045289 denotes luciferase
T12340 19512-19517 NCBITaxon_EXT:yeast denotes yeast
T12341 19533-19542 SO_EXT:0000902 denotes transgene
T12342 19548-19552 PR_EXT:P04386 denotes GAL4
T12343 19553-19560 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T12344 19553-19566 SO_EXT:0000409 denotes binding sites
T12345 19567-19575 SO_EXT:sequence_upstreamness denotes upstream
T12346 19583-19587 PR_EXT:000033987 denotes lacZ
T12347 19588-19592 SO_EXT:0000704 denotes gene
T12348 19624-19628 PR_EXT:000014441 denotes mr-s
T12349 19634-19643 SO_EXT:engineered_biological_sequence denotes construct
T12350 19652-19656 PR_EXT:000033987 denotes lacZ
T12351 19657-19667 GO:0010467 denotes expression
T12352 19689-19693 PR_EXT:000014441 denotes mr-s
T12353 19699-19708 SO_EXT:engineered_biological_sequence denotes construct
T12354 19737-19747 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T12355 19752-19762 CHEBI_SO_EXT:amino_acid denotes amino acid
T12356 19764-19766 CHEBI_SO_EXT:amino_acid denotes aa
T12357 19779-19783 PR_EXT:000014441 denotes mr-s
T12358 19814-19820 SO_EXT:0000417 denotes domain
T12359 19843-19856 GO_EXT:transcription denotes transcription
T12360 19860-19864 PR_EXT:000033987 denotes lacZ
T12361 19890-19900 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T12362 19905-19907 CHEBI_SO_EXT:amino_acid denotes aa
T12363 19919-19923 PR_EXT:000014441 denotes mr-s
T12364 19943-19956 GO_EXT:transcription denotes transcription
T12365 19960-19964 PR_EXT:000033987 denotes lacZ
T12394 21542-21546 PR_EXT:000014441 denotes mr-s
T12395 21547-21554 CHEBI_PR_EXT:protein denotes protein
T12396 21558-21567 NCBITaxon:40674 denotes mammalian
T12397 21568-21573 CL_GO_EXT:cell denotes cells
T12398 21635-21640 CL_GO_EXT:cell denotes cells
T12399 21647-21659 GO:0009294 denotes transfection
T12400 21666-21672 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12401 21685-21694 SO_EXT:sequence_truncation_process denotes truncated
T12402 21695-21699 PR_EXT:000014441 denotes mr-s
T12403 21709-21715 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12404 21728-21737 SO_EXT:sequence_truncation_process denotes truncated
T12405 21738-21742 PR_EXT:000014441 denotes mr-s
T12406 21797-21803 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12407 21804-21818 PR_EXT:000014841 denotes Sonic hedgehog
T12408 21820-21823 PR_EXT:000014841 denotes Shh
T12409 21878-21883 NCBITaxon_EXT:yeast denotes yeast
T12410 21895-21899 PR_EXT:P04386 denotes GAL4
T12411 21910-21916 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12412 21929-21933 PR_EXT:000014441 denotes mr-s
T12413 21980-21986 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12414 21999-22003 PR_EXT:000014441 denotes mr-s
T12415 22010-22013 PR_EXT:000014441 denotes mrs
T12416 22028-22034 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12417 22035-22045 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminus
T12418 22073-22079 SO_EXT:0000417 denotes domain
T3358 2255-2269 _FRAGMENT denotes development of
T3359 2284-2290 GO:0060041 denotes retina
T3360 2274-2283 NCBITaxon:40674 denotes mammalian
T3361 2284-2290 UBERON:0000966 denotes retina
T3362 2311-2315 CL_GO_EXT:cell denotes cell
T3363 2350-2361 _FRAGMENT denotes multipotent
T3364 2381-2386 CL:0000048 denotes cells
T3365 2362-2369 UBERON:0000966 denotes retinal
T3366 2362-2386 CL:0002672 denotes retinal progenitor cells
T3367 2381-2386 CL_GO_EXT:cell denotes cells
T3368 2400-2404 CL_GO_EXT:cell denotes cell
T3369 2412-2426 CL:0000210 denotes photoreceptors
T3370 2455-2460 CL_GO_EXT:cell denotes cells
T3371 2468-2474 UBERON:0000966 denotes retina
T3372 2479-2490 NCBITaxon:7742 denotes vertebrates
T3373 2517-2531 CL:0000210 denotes photoreceptors
T3374 2533-2537 CL:0000604 denotes rods
T3375 2542-2547 CL:0000573 denotes cones
T3376 2549-2553 CL:0000604 denotes Rods
T3377 2586-2591 CL:0000573 denotes cones
T3378 2647-2653 GO:0007601 denotes vision
T3379 2655-2672 GO:0007602 denotes Phototransduction
T3380 2727-2733 CHEBI:30212 denotes photon
T3381 2764-2780 GO:0016037 denotes capture of light
T3382 2786-2800 CHEBI:16066 denotes 11-cis-retinal
T3383 2804-2815 CHEBI_EXT:23240 denotes chromophore
T3384 2816-2821 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes bound
T3385 2829-2834 PR_EXT:000001119 denotes opsin
T16049 29611-29621 CHEBI_SO_EXT:amino_acid denotes amino acid
T16050 29622-29628 SO_EXT:sequence_or_structure_motif denotes motifs
T16051 29698-29702 SO_EXT:0000704 denotes gene
T16052 31233-31236 PR_EXT:000014441 denotes mrs
T16053 31252-31267 GO_EXT:transcription denotes transcriptional
T16056 31392-31402 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T16057 31411-31422 CHEBI_SO_EXT:amino_acid denotes amino acids
T20677 39353-39358 GO:0007567 denotes natal
T20678 39376-39386 GO:0010467 denotes expression
T20685 39529-39544 GO_EXT:transcription denotes transcriptional
T20686 39573-39586 GO_EXT:negative_regulation denotes down-regulate
T20687 39587-39605 CL:0000573 denotes cone photoreceptor
T20688 39615-39620 SO_EXT:0000704 denotes genes
T20689 39637-39647 CHEBI_GO_EXT:biological_process_or_quality_or_role denotes biological
T20690 39660-39664 PR_EXT:000014441 denotes mr-s
T20691 39678-39682 NCBITaxon:10088 denotes mice
T20692 39712-39716 PR_EXT:000014441 denotes mr-s
R398 T3433 T3432 _lexicallyChainedTo of photoreceptors,cell fate determination
R399 T3537 T3536 _lexicallyChainedTo genes,silencing of
R1701 T7339 T7338 _lexicallyChainedTo for genes,fragments
R3805 T13845 T13844 _lexicallyChainedTo cells,nucleus of
R4871 T20660 T20659 _lexicallyChainedTo photoreceptors,rod
R4872 T20665 T20664 _lexicallyChainedTo photoreceptors,cell fate determination of
R4873 T20666 T20664 _lexicallyChainedTo cone photoreceptors,cell fate determination of
R4874 T20676 T20675 _lexicallyChainedTo period,late embryonic
R6425 T22671 T22670 _lexicallyChainedTo photoreceptors,development of
R6426 T22672 T22670 _lexicallyChainedTo pineal gland,development of
R7869 T27839 T27838 _lexicallyChainedTo gene,fragment of
R8349 T29406 T29405 _lexicallyChainedTo retina,development of
R8350 T29413 T29412 _lexicallyChainedTo retina,layer of
R396 T3359 T3358 _lexicallyChainedTo retina,development of
R397 T3364 T3363 _lexicallyChainedTo cells,multipotent

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T822 32-36 PR:000014441 denotes mr-s
T823 50-56 SO:0000417 denotes domain
T824 80-89 GO:0010467 denotes expressed
T825 93-100 UBERON:0000966 denotes retinal
T826 93-120 CL:0010009 denotes retinal photoreceptor cells
T827 168-175 SO:0000417 denotes domains
T828 259-266 SO:0000417 denotes modules
T829 273-279 SO:0000417 denotes domain
T830 481-487 SO:0000417 denotes domain
T831 531-540 GO:0010467 denotes expressed
T832 544-551 UBERON:0000966 denotes retinal
T833 544-566 CL:0010009 denotes retinal photoreceptors
T834 575-587 UBERON:0001905 denotes pineal gland
T835 606-611 NCBITaxon:10088 denotes mouse
T836 612-616 PR:000014441 denotes mr-s
T837 618-650 PR:000014441 denotes major retinal SAM domain protein
T838 624-631 UBERON:0000966 denotes retinal
T839 636-642 SO:0000417 denotes domain
T840 662-666 PR:000014441 denotes mr-s
T841 700-709 NCBITaxon:7955 denotes zebrafish
T842 718-723 NCBITaxon:9606 denotes human
T843 725-734 NCBITaxon:1 denotes organisms
T844 766-779 CL:0000210 denotes photoreceptor
T845 766-791 GO:0042461 denotes photoreceptor development
T846 862-868 SO:0000417 denotes domain
T847 872-876 PR:000014441 denotes mr-s
T848 906-911 NCBITaxon:10088 denotes mouse
T849 930-938 SO:0000855 denotes ortholog
T850 940-944 PR:Q64028 denotes Mph1
T851 945-950 PR:000012633 denotes Rae28
T852 984-992 CHEBI:36357 denotes molecule
T853 1005-1014 GO:0000785 denotes chromatin
T854 1005-1028 GO:0016568 denotes chromatin modifications
T855 1074-1078 PR:000014441 denotes mr-s
T856 1107-1117 GO:0065007 denotes regulating
T857 1118-1122 SO:0000704 denotes gene
T858 1118-1133 GO:0010467 denotes gene expression
T859 1137-1150 CL:0000210 denotes photoreceptor
T860 1137-1162 GO:0042461 denotes photoreceptor development
T861 1164-1168 PR:000014441 denotes mr-s
T862 1187-1196 GO:0010467 denotes expressed
T863 1204-1218 CL:0000210 denotes photoreceptors
T864 1226-1231 GO:0007567 denotes natal
T865 1253-1267 CL:0000210 denotes photoreceptors
T866 1313-1318 UBERON:0007023 denotes adult
T867 1319-1331 UBERON:0001905 denotes pineal gland
T868 1350-1354 PR:000014441 denotes mr-s
T869 1367-1376 GO:0065007 denotes regulated
T870 1384-1388 CL:0000573 denotes cone
T871 1389-1392 CL:0000604 denotes rod
T872 1413-1416 PR:000005904 denotes Crx
T873 1460-1464 PR:000014441 denotes mr-s
T874 1512-1518 SO:0000417 denotes domain
T875 1556-1560 PR:000014441 denotes mr-s
T876 1593-1600 GO:0005634 denotes nucleus
T877 1607-1611 PR:000014441 denotes mr-s
T878 1619-1628 GO:0010467 denotes expressed
T879 1697-1701 PR:000014441 denotes mr-s
T880 1719-1723 PR:P04386 denotes GAL4
T881 1736-1742 SO:0000417 denotes domain
T882 1829-1833 PR:000014441 denotes mr-s
T883 1889-1895 SO:0000417 denotes domain
T884 1960-1964 SO:0000704 denotes gene
T885 1966-1970 PR:000014441 denotes mr-s
T886 1995-2004 GO:0010467 denotes expressed
T887 2008-2015 UBERON:0000966 denotes retinal
T888 2008-2030 CL:0010009 denotes retinal photoreceptors
T889 2035-2047 UBERON:0001905 denotes pineal gland
T890 2062-2072 GO:0010467 denotes expression
T891 2123-2127 PR:000014441 denotes mr-s
T892 2175-2194 CL:0000210 denotes photoreceptor cells
T893 2202-2214 CL:0000652 denotes pinealocytes
T894 2222-2234 UBERON:0001905 denotes pineal gland
T3221 2858-2862 CL:0000604 denotes rods
T3222 2867-2871 CL:0000573 denotes cone
T3223 2872-2878 PR:000001119 denotes opsins
T3224 2882-2887 CL:0000573 denotes cones
T3225 2917-2934 GO:0007602 denotes phototransduction
T3226 2986-2996 GO:0005886 denotes membranous
T3227 3012-3025 GO:0001750 denotes outer segment
T3228 3031-3044 GO:0001750 denotes outer segment
T3229 3133-3140 SO:0000704 denotes genetic
T3230 3163-3169 NCBITaxon:9989 denotes rodent
T3231 3170-3176 UBERON:0000966 denotes retina
T3232 3272-3289 CL:0000604 denotes Rod photoreceptor
T3233 3272-3300 GO:0046548 denotes Rod photoreceptor generation
T3234 3326-3331 GO:0007567 denotes birth
T3235 3333-3352 CL:0000573 denotes Cone photoreceptors
T3236 3354-3362 UBERON:0000045 denotes ganglion
T3237 3354-3368 CL:0000740 denotes ganglion cells
T3238 3370-3386 CL:0000745 denotes horizontal cells
T3239 3391-3405 CL:0000561 denotes amacrine cells
T3240 3435-3446 CL:0011107 denotes Müller glia
T3241 3451-3464 CL:0000103 denotes bipolar cells
T3242 3661-3670 GO:0000785 denotes chromatin
T3243 3661-3683 GO:0016568 denotes chromatin modification
T3244 3754-3767 CL:0000210 denotes photoreceptor
T3245 3754-3779 GO:0042461 denotes photoreceptor development
T3246 3811-3816 SO:0000704 denotes genes
T3247 3818-3822 PR:000012086 denotes Otx2
T3248 3827-3830 PR:000005904 denotes Crx
T3249 3862-3885 _FRAGMENT denotes cell fate determination
T3250 3915-3932 GO:0043703 denotes of photoreceptors
T3251 3918-3932 CL:0000210 denotes photoreceptors
T3252 3960-3964 PR:000012086 denotes Otx2
T3253 3991-4010 CL:0000210 denotes photoreceptor cells
T3254 4028-4036 CL:0000561 denotes amacrine
T3255 4042-4049 CL:0000540 denotes neurons
T3256 4055-4058 PR:000005904 denotes Crx
T3257 4083-4087 PR:000012086 denotes Otx2
T3258 4089-4097 GO:0065007 denotes controls
T3259 4127-4145 CL:0000210 denotes photoreceptor cell
T3260 4155-4160 SO:0000704 denotes genes
T3261 4199-4213 GO:0001750 denotes outer segments
T3262 4215-4223 GO:0045202 denotes synaptic
T3263 4239-4256 GO:0007602 denotes phototransduction
T3264 4274-4277 PR:000005904 denotes Crx
T3265 4278-4289 SO:0000673 denotes transcripts
T3266 4302-4311 GO:0010467 denotes expressed
T3267 4326-4340 CL:0000210 denotes photoreceptors
T3268 4344-4353 UBERON:0000922 denotes embryonic
T3269 4378-4383 NCBITaxon:10088 denotes mouse
T3270 4413-4416 PR:000005904 denotes Crx
T3271 4470-4484 CL:0000210 denotes photoreceptors
T3272 4492-4497 GO:0007567 denotes natal
T3273 4510-4529 CL:0000210 denotes Photoreceptor cells
T3274 4537-4540 PR:000005904 denotes Crx
T3275 4555-4559 NCBITaxon:10088 denotes mice
T3276 4597-4610 CL:0000210 denotes photoreceptor
T3277 4611-4620 CHEBI:36357 denotes molecules
T3278 4631-4637 GO:0007601 denotes visual
T3279 4638-4646 CHEBI:26130 denotes pigments
T3280 4667-4680 CL:0000210 denotes photoreceptor
T3281 4667-4695 GO:0001750 denotes photoreceptor outer segments
T3282 4702-4710 GO:0045202 denotes synaptic
T3283 4754-4759 NCBITaxon:9606 denotes human
T3284 4760-4763 PR:000005904 denotes CRX
T3285 4824-4837 CL:0000210 denotes photoreceptor
T3286 4848-4857 GO:0030849 denotes autosomal
T3287 4867-4871 CL:0000573 denotes cone
T3288 4872-4875 CL:0000604 denotes rod
T3289 4889-4898 GO:0030849 denotes autosomal
T3290 4989-4993 PR:000012086 denotes Otx2
T3291 4998-5001 PR:000005904 denotes Crx
T3292 5002-5009 GO:0065007 denotes control
T3293 5018-5031 CL:0000210 denotes photoreceptor
T3294 5018-5043 GO:0042461 denotes photoreceptor development
T3295 5080-5084 PR:000016321 denotes TRβ2
T3296 5086-5089 PR:000011432 denotes Nrl
T3297 5095-5100 PR:000011403 denotes Nr2e3
T3298 5101-5109 GO:0065007 denotes regulate
T3299 5131-5149 CL:0000210 denotes photoreceptor cell
T3300 5169-5176 SO:0000417 denotes domains
T3301 5213-5220 SO:0000417 denotes domains
T3302 5305-5312 SO:0000417 denotes modules
T3303 5327-5333 SO:0000417 denotes domain
T3304 5498-5501 PR:000016568 denotes p73
T3305 5549-5554 PR:Q23972 denotes Smaug
T3306 5561-5575 CHEBI:18035 denotes diacylglycerol
T3307 5599-5605 GO:0007618 denotes mating
T3308 5746-5750 SO:0000704 denotes gene
T3309 5746-5760 GO:0016458 denotes gene silencing
T3310 5792-5799 NCBITaxon:40674 denotes mammals
T3311 5841-5845 SO:0000704 denotes gene
T3312 5846-5856 GO:0065007 denotes regulation
T3313 5899-5911 _FRAGMENT denotes silencing of
T3314 5929-5934 GO:0016458 denotes genes
T3315 5929-5934 SO:0000704 denotes genes
T3316 5943-5952 GO:0000785 denotes chromatin
T3317 5943-5966 GO:0016568 denotes chromatin modifications
T3318 5980-6009 GO:0035102 denotes polycomb repressive complex 1
T3319 6011-6015 GO:0035102 denotes PRC1
T3320 6037-6043 SO:0000417 denotes domain
T3321 6067-6079 GO:0035102 denotes PRC1 complex
T3322 6097-6104 SO:0001114 denotes helical
T3323 6119-6127 CHEBI:33839 denotes polymers
T3324 6144-6150 SO:0000417 denotes domain
T3325 6163-6172 CHEBI:33839 denotes polymeric
T3326 6224-6233 GO:0000785 denotes chromatin
T3327 6294-6299 SO:0000704 denotes genes
T3328 6338-6342 SO:0000704 denotes gene
T3329 6344-6348 PR:000014441 denotes mr-s
T3330 6370-6376 SO:0000417 denotes domain
T3331 6405-6411 SO:0000417 denotes domain
T3332 6415-6419 PR:000014441 denotes mr-s
T3333 6458-6463 NCBITaxon:10088 denotes mouse
T3334 6464-6472 SO:0000855 denotes ortholog
T3335 6474-6478 PR:Q64028 denotes MPH1
T3336 6479-6484 PR:000012633 denotes Rae28
T3337 6486-6490 PR:000014441 denotes mr-s
T3338 6508-6517 GO:0010467 denotes expressed
T3339 6521-6528 UBERON:0000966 denotes retinal
T3340 6521-6543 CL:0010009 denotes retinal photoreceptors
T3341 6592-6597 UBERON:0007023 denotes adult
T3342 6598-6610 UBERON:0001905 denotes pineal gland
T3343 6616-6626 GO:0010467 denotes expression
T3344 6630-6634 PR:000014441 denotes mr-s
T3345 6647-6656 GO:0065007 denotes regulated
T3346 6660-6663 PR:000005904 denotes Crx
T3347 6675-6679 PR:000014441 denotes mr-s
T3348 6700-6707 GO:0005634 denotes nucleus
T3349 6747-6753 SO:0000417 denotes domain
T3350 6792-6796 PR:000014441 denotes mr-s
T3351 6814-6818 PR:P04386 denotes GAL4
T3352 6831-6837 SO:0000417 denotes domain
T3353 6908-6912 PR:000014441 denotes mr-s
T3354 6940-6973 GO:0017053 denotes transcriptional repressor complex
T3355 6977-6984 UBERON:0000966 denotes retinal
T3356 6977-6998 CL:0010009 denotes retinal photoreceptor
T3357 6985-7010 GO:0042461 denotes photoreceptor development
T5369 7033-7038 NCBITaxon:10088 denotes mouse
T5370 7039-7043 PR:000014441 denotes mr-s
T5371 7071-7076 NCBITaxon:10088 denotes mouse
T5372 7077-7082 SO:0000704 denotes genes
T5373 7098-7107 GO:0010467 denotes expressed
T5374 7126-7132 UBERON:0000966 denotes retina
T5375 7276-7279 SO:0000345 denotes EST
T5376 7322-7327 NCBITaxon:10088 denotes mouse
T5377 7328-7335 UBERON:0000966 denotes retinal
T5378 7426-7432 SO:0000417 denotes domain
T5379 7511-7516 NCBITaxon:10088 denotes mouse
T5380 7544-7549 NCBITaxon:10088 denotes mouse
T5381 7556-7563 UBERON:0000966 denotes retinal
T5382 7601-7611 SO:0000317 denotes cDNA clone
T5383 7665-7669 SO:0000704 denotes gene
T5384 7685-7691 SO:0000417 denotes domain
T5385 7743-7747 PR:000014441 denotes mr-s
T5386 7749-7781 PR:000014441 denotes major retinal SAM domain protein
T5387 7755-7762 UBERON:0000966 denotes retinal
T5388 7767-7773 SO:0000417 denotes domain
T5389 7807-7829 GO:0006413 denotes translation initiation
T5390 7807-7835 SO:0000318 denotes translation initiation codon
T5391 7859-7877 SO:0000236 denotes open reading frame
T5392 7889-7895 SO:0000417 denotes domain
T5393 7902-7917 SO:0000318 denotes initiation site
T5394 7942-7951 SO:0000993 denotes consensus
T5395 8058-8068 SO:0000319 denotes stop codon
T5396 8086-8090 PR:000014441 denotes mr-s
T5397 8164-8170 SO:0000417 denotes domain
T5398 8174-8178 PR:000014441 denotes mr-s
T5399 8222-8230 SO:0000857 denotes homology
T5400 8240-8247 SO:0000417 denotes domains
T5401 8251-8256 PR:000007130 denotes EphB2
T5402 8258-8263 PR:000007124 denotes EphA4
T5403 8265-8269 PR:Q64028 denotes MPH1
T5404 8271-8274 PR:000007229 denotes TEL
T5405 8279-8284 PR:Q23972 denotes Smaug
T5406 8330-8336 SO:0000417 denotes domain
T5407 8340-8344 PR:000014441 denotes mr-s
T5408 8380-8384 PR:Q64028 denotes Mph1
T5409 8385-8390 PR:000012633 denotes Rae28
T5410 8394-8399 NCBITaxon:10088 denotes mouse
T5411 8400-8407 SO:0000853 denotes homolog
T5412 8425-8430 NCBITaxon:10088 denotes Mouse
T5413 8431-8435 PR:000014441 denotes mr-s
T5414 8460-8463 NCBITaxon:10114 denotes rat
T5415 8465-8470 NCBITaxon:9606 denotes human
T5416 8482-8491 NCBITaxon:7955 denotes zebrafish
T5417 8543-8548 NCBITaxon:10088 denotes mouse
T5418 8549-8553 PR:000014441 denotes mr-s
T5419 8587-8594 SO:0000417 denotes domains
T5420 8598-8601 NCBITaxon:10114 denotes rat
T5421 8603-8608 NCBITaxon:9606 denotes human
T5422 8620-8629 NCBITaxon:7955 denotes zebrafish
T5423 8630-8634 PR:000014441 denotes mr-s
T5424 8720-8726 SO:0000417 denotes domain
T5425 8730-8735 NCBITaxon:10088 denotes mouse
T5426 8736-8740 PR:000014441 denotes mr-s
T5427 8783-8788 NCBITaxon:10088 denotes mouse
T5428 8793-8798 NCBITaxon:9606 denotes human
T5429 8799-8803 PR:000014441 denotes mr-s
T5430 8804-8809 SO:0000704 denotes genes
T5431 8843-8848 NCBITaxon:10088 denotes mouse
T5432 8853-8858 NCBITaxon:9606 denotes human
T5433 8859-8865 SO:0001026 denotes genome
T5434 8898-8903 NCBITaxon:10088 denotes Mouse
T5435 8904-8908 PR:000014441 denotes mr-s
T5436 8942-8947 NCBITaxon:9606 denotes human
T5437 8948-8952 PR:000014441 denotes MR-S
T5438 9049-9054 NCBITaxon:9606 denotes Human
T5439 9055-9059 PR:000014441 denotes MR-S
T7136 10257-10267 GO:0010467 denotes Expression
T7137 10271-10275 PR:000014441 denotes mr-s
T7138 10294-10300 UBERON:0000966 denotes retina
T7139 10309-10321 UBERON:0001905 denotes pineal gland
T7140 10337-10341 PR:000014441 denotes mr-s
T7141 10342-10352 GO:0010467 denotes expression
T7142 10393-10406 GO:0097617 denotes hybridization
T7143 10410-10415 NCBITaxon:10088 denotes mouse
T7144 10416-10423 UBERON:0000922 denotes embryos
T7145 10428-10441 GO:0097617 denotes hybridization
T7146 10495-10499 PR:000014441 denotes mr-s
T7147 10549-10559 GO:0010467 denotes expression
T7148 10567-10571 PR:000014441 denotes mr-s
T7149 10572-10576 SO:0000704 denotes gene
T7150 10595-10601 UBERON:0000966 denotes retina
T7151 10621-10634 GO:0097617 denotes hybridization
T7152 10701-10707 UBERON:0000966 denotes retina
T7153 10790-10802 CL:0000031 denotes neuroblastic
T7154 10830-10843 CL:0000210 denotes photoreceptor
T7155 10830-10849 UBERON:0001787 denotes photoreceptor layer
T7156 10885-10889 PR:000014441 denotes mr-s
T7157 10890-10900 SO:0000673 denotes transcript
T7158 10940-10953 CL:0000210 denotes photoreceptor
T7159 10940-10959 UBERON:0001787 denotes photoreceptor layer
T7160 10978-10982 PR:000014441 denotes mr-s
T7161 10995-11005 GO:0010467 denotes expression
T7162 11013-11026 CL:0000210 denotes photoreceptor
T7163 11013-11032 UBERON:0001787 denotes photoreceptor layer
T7164 11101-11111 GO:0010467 denotes expressing
T7165 11112-11121 PR:000001245 denotes rhodopsin
T7210 13172-13183 SO:0000673 denotes transcripts
T7211 13197-13203 UBERON:0000922 denotes embryo
T7212 13205-13215 UBERON:0000468 denotes whole body
T7213 13217-13223 UBERON:0000966 denotes retina
T7214 13225-13237 UBERON:0001905 denotes pineal gland
T7215 13239-13244 UBERON:0000955 denotes brain
T7216 13246-13251 UBERON:0002107 denotes liver
T7217 13262-13268 UBERON:0000062 denotes organs
T7218 13344-13353 _FRAGMENT denotes fragments
T7219 13393-13402 SO:0000997 denotes for genes
T7220 13361-13363 SO:0000028 denotes bp
T7221 13372-13374 SO:0000028 denotes bp
T7222 13380-13386 SO:0000112 denotes primer
T7223 13412-13417 NCBITaxon:10088 denotes mouse
T7224 13418-13422 PR:000014441 denotes mr-s
T7225 13461-13467 UBERON:0000922 denotes embryo
T7226 13475-13485 UBERON:0000468 denotes whole body
T7227 13502-13505 UBERON:0000970 denotes eye
T7228 13511-13515 PR:000014441 denotes mr-s
T7229 13567-13571 PR:000014441 denotes mr-s
T7230 13575-13584 GO:0010467 denotes expressed
T7231 13599-13604 UBERON:0007023 denotes adult
T7232 13605-13617 UBERON:0001905 denotes pineal gland
T7233 13633-13638 UBERON:0007023 denotes adult
T7234 13639-13644 UBERON:0000955 denotes brain
T7235 13646-13651 UBERON:0002107 denotes liver
T7236 13670-13676 UBERON:0000062 denotes organs
T7237 13707-13711 PR:000014441 denotes mr-s
T7238 13780-13784 PR:000014441 denotes mr-s
T7239 13802-13811 GO:0010467 denotes expressed
T7240 13826-13840 CL:0000210 denotes photoreceptors
T7241 13849-13861 UBERON:0001905 denotes pineal gland
T9401 13864-13874 GO:0065007 denotes Regulation
T9402 13878-13882 PR:000014441 denotes mr-s
T9403 13900-13903 PR:000005904 denotes Crx
T9404 13946-13950 PR:000012086 denotes Otx2
T9405 13955-13958 PR:000005904 denotes Crx
T9406 13993-14000 UBERON:0000966 denotes retinal
T9407 13993-14014 CL:0010009 denotes retinal photoreceptor
T9408 14001-14026 GO:0042461 denotes photoreceptor development
T9409 14050-14054 PR:000012086 denotes Otx2
T9410 14059-14062 PR:000005904 denotes Crx
T9411 14067-14076 GO:0010467 denotes expressed
T9412 14091-14110 CL:0000210 denotes photoreceptor cells
T9413 14118-14127 UBERON:0000922 denotes embryonic
T9414 14128-14134 UBERON:0000966 denotes retina
T9415 14141-14144 PR:000005904 denotes Crx
T9416 14155-14164 GO:0010467 denotes expressed
T9417 14176-14181 GO:0007567 denotes natal
T9418 14182-14196 CL:0000210 denotes photoreceptors
T9419 14214-14218 PR:000014441 denotes mr-s
T9420 14226-14235 GO:0065007 denotes regulated
T9421 14239-14242 PR:000005904 denotes Crx
T9422 14260-14263 PR:000005904 denotes Crx
T9423 14264-14273 GO:0065007 denotes regulates
T9424 14274-14278 PR:000014441 denotes mr-s
T9425 14315-14328 GO:0097617 denotes hybridization
T9426 14332-14336 PR:000014441 denotes mr-s
T9427 14359-14362 PR:000005904 denotes Crx
T9428 14369-14376 UBERON:0000966 denotes retinas
T9429 14398-14401 PR:000005904 denotes Crx
T9430 14405-14411 UBERON:0000966 denotes retina
T9431 14417-14421 PR:000014441 denotes mr-s
T9432 14422-14432 SO:0000673 denotes transcript
T9433 14489-14492 PR:000005904 denotes Crx
T9434 14529-14533 PR:000014441 denotes mr-s
T9435 14582-14586 PR:000014441 denotes mr-s
T9436 14595-14604 GO:0065007 denotes regulated
T9437 14608-14611 PR:000005904 denotes Crx
T9438 14619-14631 UBERON:0001905 denotes pineal gland
T9439 14695-14699 PR:000014441 denotes mr-s
T9440 14721-14724 PR:000005904 denotes Crx
T9441 14728-14740 UBERON:0001905 denotes pineal gland
T9442 14778-14782 PR:000014441 denotes mr-s
T9443 14836-14839 PR:000005904 denotes Crx
T9444 14843-14855 UBERON:0001905 denotes pineal gland
T9445 14898-14902 PR:000014441 denotes mr-s
T9446 14920-14929 GO:0065007 denotes regulated
T9447 14933-14936 PR:000005904 denotes Crx
T9448 14955-14969 CL:0000210 denotes photoreceptors
T9449 14974-14986 UBERON:0001905 denotes pineal gland
T9450 16033-16034 GO:0065007 denotes u
T9451 16034-16035 PR:000005904 denotes r
T9452 16036-16037 GO:0097617 denotes h
T9453 16040-16041 NCBITaxon:10088 denotes e
T9454 16041-16042 PR:000005904 denotes x
T9455 16042-16043 UBERON:0000966 denotes a
T9478 16514-16524 GO:0010467 denotes expression
T9479 16525-16531 SO:0000440 denotes vector
T9480 16638-16641 PR:000011432 denotes Nrl
T9481 16642-16652 GO:0010467 denotes expression
T9482 16653-16659 SO:0000440 denotes vector
T9483 16744-16747 PR:000005904 denotes Crx
T9484 16766-16769 PR:000011432 denotes Nrl
T9485 16779-16788 PR:000001245 denotes rhodopsin
T9486 16789-16797 SO:0000167 denotes promoter
T9487 16821-16825 SO:0000704 denotes gene
T9488 16894-16898 SO:0000704 denotes gene
T9489 16894-16909 GO:0010467 denotes gene expression
T9490 16940-16943 PR:000005904 denotes Crx
T9491 16948-16951 PR:000011432 denotes Nrl
T9492 16952-16962 GO:0010467 denotes expression
T9493 16963-16970 SO:0000440 denotes vectors
T9494 17037-17040 PR:000005904 denotes Crx
T9495 17046-17056 GO:0010467 denotes expression
T9496 17057-17063 SO:0000440 denotes vector
T9497 17144-17151 UBERON:0000966 denotes retinal
T9498 17152-17158 UBERON:0001905 denotes pineal
T9499 17246-17250 PR:000012086 denotes Otx2
T9500 17295-17304 SO:0000994 denotes consensus
T9501 17308-17311 PR:000005904 denotes Crx
T9502 17333-17337 PR:000014441 denotes mr-s
T9503 17338-17348 GO:0010467 denotes expression
T9504 17377-17387 GO:0010467 denotes expression
T9505 17399-17403 PR:000014441 denotes mr-s
T9506 17428-17431 PR:000005904 denotes Crx
T9507 17450-17461 SO:0000673 denotes transcripts
T9508 17465-17469 PR:000012086 denotes Otx2
T9509 17497-17510 CL:0000210 denotes photoreceptor
T9510 17497-17516 UBERON:0001787 denotes photoreceptor layer
T9511 17520-17529 UBERON:0000922 denotes embryonic
T9512 17567-17571 PR:000014441 denotes mr-s
T9513 17598-17607 GO:0065007 denotes regulated
T9514 17618-17621 PR:000005904 denotes Crx
T9515 17652-17659 SO:0000440 denotes vectors
T9516 17708-17711 PR:000005904 denotes Crx
T9517 17712-17725 SO:0000409 denotes binding sites
T9518 17779-17782 PR:000005904 denotes Crx
T9519 17783-17793 GO:0010467 denotes expression
T9520 17794-17800 SO:0000440 denotes vector
T9521 17915-17918 PR:000005904 denotes Crx
T9522 18018-18029 GO:0009294 denotes transfected
T9523 18063-18066 PR:000005904 denotes Crx
T9524 18115-18118 PR:000005904 denotes Crx
T12205 18703-18707 PR:000014441 denotes mr-s
T12206 18820-18825 NCBITaxon:10088 denotes mouse
T12207 18832-18839 UBERON:0000966 denotes retinal
T12208 18864-18868 PR:P04386 denotes GAL4
T12209 18880-18886 SO:0000417 denotes domain
T12210 18975-18981 SO:0000417 denotes domain
T12211 18985-18989 PR:000014441 denotes mr-s
T12212 19036-19040 PR:000014441 denotes mr-s
T12213 19077-19083 SO:0000417 denotes domain
T12214 19153-19157 PR:000014441 denotes mr-s
T12215 19226-19230 PR:000014441 denotes mr-s
T12216 19258-19264 SO:0000417 denotes domain
T12217 19299-19303 PR:P04386 denotes GAL4
T12218 19379-19383 PR:000014441 denotes mr-s
T12219 19399-19403 PR:P04386 denotes GAL4
T12220 19431-19437 SO:0000417 denotes domain
T12221 19495-19506 GO:0009294 denotes transformed
T12222 19533-19542 SO:0000902 denotes transgene
T12223 19548-19552 PR:P04386 denotes GAL4
T12224 19553-19566 SO:0000409 denotes binding sites
T12225 19583-19587 PR:000033987 denotes lacZ
T12226 19588-19592 SO:0000704 denotes gene
T12227 19624-19628 PR:000014441 denotes mr-s
T12228 19652-19656 PR:000033987 denotes lacZ
T12229 19657-19667 GO:0010467 denotes expression
T12230 19689-19693 PR:000014441 denotes mr-s
T12231 19779-19783 PR:000014441 denotes mr-s
T12232 19814-19820 SO:0000417 denotes domain
T12233 19860-19864 PR:000033987 denotes lacZ
T12234 19919-19923 PR:000014441 denotes mr-s
T12235 19960-19964 PR:000033987 denotes lacZ
T12236 20015-20019 PR:000014441 denotes mr-s
T12237 20069-20073 PR:000014441 denotes mr-s
T12238 20099-20103 PR:000033987 denotes lacZ
T12239 20182-20186 PR:000033987 denotes lacZ
T12240 20187-20197 GO:0010467 denotes expression
T12241 20272-20276 PR:000014441 denotes mr-s
T12242 20309-20313 PR:P04386 denotes GAL4
T12243 20367-20371 PR:000014441 denotes mr-s
T12244 20453-20460 SO:0000417 denotes domains
T12245 20558-20565 GO:0005634 denotes nucleus
T12246 20577-20581 PR:P04386 denotes GAL4
T12247 20582-20595 SO:0000409 denotes binding sites
T12248 20620-20626 SO:0000417 denotes domain
T12249 21542-21546 PR:000014441 denotes mr-s
T12250 21558-21567 NCBITaxon:40674 denotes mammalian
T12251 21647-21659 GO:0009294 denotes transfection
T12252 21695-21699 PR:000014441 denotes mr-s
T12253 21738-21742 PR:000014441 denotes mr-s
T12254 21804-21818 PR:000014841 denotes Sonic hedgehog
T12255 21820-21823 PR:000014841 denotes Shh
T12256 21895-21899 PR:P04386 denotes GAL4
T12257 21929-21933 PR:000014441 denotes mr-s
T12258 21999-22003 PR:000014441 denotes mr-s
T12259 22010-22013 PR:000014441 denotes mrs
T12260 22073-22079 SO:0000417 denotes domain
T12261 22187-22199 GO:0009294 denotes transfection
T12262 22249-22253 PR:000014441 denotes mr-s
T12263 22338-22349 GO:0009294 denotes transfected
T12264 22395-22398 PR:000014441 denotes mrs
T12275 24106-24110 PR:P04386 denotes GAL4
T12276 24136-24140 PR:000014441 denotes mr-s
T12277 24190-24196 SO:0000417 denotes domain
T12278 24251-24257 SO:0000417 denotes domain
T13791 24292-24296 PR:000014441 denotes mr-s
T13792 24308-24317 NCBITaxon:40674 denotes mammalian
T13793 24337-24344 GO:0005634 denotes nuclear
T13794 24337-24364 SO:0001528 denotes nuclear localization signal
T13795 24390-24394 PR:000014441 denotes mr-s
T13796 24443-24447 PR:000014441 denotes mr-s
T13797 24473-24480 GO:0005634 denotes nucleus
T13798 24527-24531 PR:000014441 denotes mr-s
T13799 24535-24544 NCBITaxon:40674 denotes mammalian
T13800 24591-24595 PR:000014441 denotes mr-s
T13801 24596-24603 SO:0000155 denotes plasmid
T13802 24660-24664 PR:000014441 denotes mr-s
T13803 24697-24707 _FRAGMENT denotes nucleus of
T13804 24716-24721 GO:0005634 denotes cells
T13805 24779-24783 PR:000014441 denotes mr-s
T13806 24805-24809 PR:000014441 denotes mr-s
T13807 24836-24840 CHEBI:51231 denotes DAPI
T13808 24881-24885 PR:000014441 denotes mr-s
T13809 24926-24933 GO:0005634 denotes nucleus
T13810 24937-24946 NCBITaxon:40674 denotes mammalian
T13811 24983-24987 PR:000014441 denotes mr-s
T13812 25027-25037 GO:0065007 denotes regulation
T13813 25055-25058 PR:000007229 denotes TEL
T15828 25362-25366 PR:P04386 denotes GAL4
T15829 25367-25371 PR:000014441 denotes mr-s
T15830 25582-25596 MOP:0000629 denotes polymerization
T15831 25716-25725 GO:0000785 denotes chromatin
T15832 25741-25745 PR:000014441 denotes mr-s
T15833 25796-25802 SO:0000417 denotes domain
T15834 25848-25852 PR:000014441 denotes mr-s
T15835 25905-25913 CHEBI:35224 denotes effector
T15836 25914-25922 SO:0000155 denotes plasmids
T15837 25930-25937 GO:0010467 denotes express
T15838 25969-25973 PR:000014441 denotes mr-s
T15839 25987-25991 PR:P04386 denotes GAL4
T15840 26004-26010 SO:0000417 denotes domain
T15841 26058-26062 PR:000014441 denotes mr-s
T15842 26072-26076 PR:P04386 denotes GAL4
T15843 26089-26095 SO:0000417 denotes domain
T15844 26101-26104 PR:000014441 denotes mrs
T15845 26132-26140 SO:0000167 denotes promoter
T15846 26141-26148 SO:0000155 denotes plasmid
T15847 26157-26161 PR:P04386 denotes GAL4
T15848 26162-26175 SO:0000409 denotes binding sites
T15849 26224-26231 SO:0000155 denotes plasmid
T15850 26239-26250 GO:0009294 denotes transfected
T15851 26256-26259 PR:000014441 denotes mrs
T15852 26355-26359 PR:P04386 denotes GAL4
T15853 26372-26378 SO:0000417 denotes domain
T15854 26439-26446 SO:0000155 denotes plasmid
T15855 26491-26495 PR:000014441 denotes mr-s
T15856 26504-26508 PR:P04386 denotes GAL4
T15857 26548-26555 SO:0000155 denotes plasmid
T15858 26653-26657 PR:000014441 denotes mr-s
T15859 26750-26754 PR:P04386 denotes GAL4
T15860 26875-26879 SO:0000704 denotes gene
T15861 26875-26890 GO:0010467 denotes gene expression
T15862 26958-26961 PR:000014441 denotes mrs
T15863 27042-27048 SO:0000417 denotes domain
T15864 27103-27107 PR:000014441 denotes mr-s
T15865 27294-27297 PR:000014441 denotes mrs
T15866 27398-27402 PR:000014441 denotes mr-s
T15867 27592-27596 PR:000014441 denotes mr-s
T15868 27730-27734 PR:P04386 denotes GAL4
T15869 27881-27888 SO:0000155 denotes plasmid
T15870 27996-28007 GO:0009294 denotes transfected
T15871 28027-28034 SO:0000155 denotes plasmid
T15872 28099-28103 PR:000014441 denotes mr-s
T15873 28116-28123 UBERON:0000966 denotes retinal
T15874 28171-28176 NCBITaxon:9606 denotes human
T15875 28234-28237 PR:000014441 denotes mrs
T15876 28481-28493 GO:0009294 denotes transfection
T15877 28559-28572 GO:0005622 denotes intracellular
T15878 28673-28687 CL:0000210 denotes photoreceptors
T15879 28784-28788 PR:000014441 denotes mr-s
T15880 28926-28930 PR:000014441 denotes mr-s
T15881 28986-29000 CL:0000210 denotes photoreceptors
T15882 29054-29058 PR:000014441 denotes mr-s
T15883 29130-29134 PR:000014441 denotes mr-s
T15884 29147-29153 SO:0000417 denotes domain
T15885 29236-29241 NCBITaxon:10088 denotes mouse
T15886 29242-29246 PR:000014441 denotes mr-s
T15887 29273-29280 NCBITaxon:species denotes species
T15888 29358-29361 NCBITaxon:10114 denotes rat
T15889 29363-29368 NCBITaxon:9606 denotes human
T15890 29380-29389 NCBITaxon:7955 denotes zebrafish
T15891 29468-29473 NCBITaxon:10088 denotes mouse
T15892 29474-29478 PR:000014441 denotes mr-s
T15893 29521-29527 SO:0000417 denotes domain
T15894 29531-29544 CL:0000210 denotes photoreceptor
T15895 29531-29556 GO:0042461 denotes photoreceptor development
T15896 29698-29702 SO:0000704 denotes gene
T15897 31233-31236 PR:000014441 denotes mrs
T15898 31314-31318 PR:000014441 denotes mr-s
T15899 31374-31380 SO:0000417 denotes domain
T20136 31492-31496 SO:0000704 denotes gene
T20137 31498-31502 PR:000014441 denotes mr-s
T20138 31527-31536 GO:0010467 denotes expressed
T20139 31540-31547 UBERON:0000966 denotes retinal
T20140 31540-31562 CL:0010009 denotes retinal photoreceptors
T20141 31571-31583 UBERON:0001905 denotes pineal gland
T20142 31597-31601 PR:000014441 denotes mr-s
T20143 31602-31612 GO:0010467 denotes expression
T20144 31631-31637 UBERON:0000966 denotes retina
T20145 31657-31667 GO:0010467 denotes expression
T20146 31714-31717 PR:000005904 denotes Crx
T20147 31719-31728 PR:000001245 denotes rhodopsin
T20148 31739-31752 CL:0000210 denotes photoreceptor
T20149 31753-31758 SO:0000704 denotes genes
T20150 31788-31809 UBERON:0001790 denotes outer plexiform layer
T20151 31840-31855 UBERON:0001781 denotes layer of retina
T20152 31883-31886 UBERON:0001789 denotes ONL
T20153 31891-31894 UBERON:0001791 denotes INL
T20154 31914-31928 CL:0000210 denotes photoreceptors
T20155 31984-31997 GO:0001750 denotes outer segment
T20156 32030-32034 PR:000014441 denotes mr-s
T20157 32044-32052 CHEBI:36357 denotes molecule
T20158 32065-32094 GO:0042461 denotes development of photoreceptors
T20159 32080-32094 CL:0000210 denotes photoreceptors
T20160 32124-32128 PR:000012086 denotes Otx2
T20161 32133-32136 PR:000005904 denotes Crx
T20162 32161-32174 CL:0000210 denotes photoreceptor
T20163 32161-32186 GO:0042461 denotes photoreceptor development
T20164 32196-32200 PR:000012086 denotes Otx2
T20165 32210-32219 GO:0065007 denotes regulates
T20166 32220-32223 PR:000005904 denotes Crx
T20167 32253-32266 GO:0097617 denotes hybridization
T20168 32310-32314 PR:000014441 denotes mr-s
T20169 32329-32332 PR:000005904 denotes Crx
T20170 32336-32342 UBERON:0000966 denotes retina
T20171 32347-32359 UBERON:0001905 denotes pineal gland
T20172 32413-32417 PR:000012086 denotes Otx2
T20173 32422-32425 PR:000005904 denotes Crx
T20174 32471-32475 PR:000014441 denotes mr-s
T20175 32479-32488 NCBITaxon:40674 denotes mammalian
T20176 32499-32506 UBERON:0000966 denotes retinal
T20177 32499-32526 CL:0010009 denotes retinal photoreceptor cells
T20178 32532-32536 PR:000012086 denotes Otx2
T20179 32537-32548 SO:0000673 denotes transcripts
T20180 32564-32573 GO:0010467 denotes expressed
T20181 32594-32597 PR:000005904 denotes Crx
T20182 32598-32609 SO:0000673 denotes transcripts
T20183 32688-32692 PR:000014441 denotes mr-s
T20184 32719-32728 GO:0065007 denotes regulated
T20185 32732-32735 PR:000005904 denotes Crx
T20186 32759-32762 PR:000011432 denotes Nrl
T20187 32766-32779 CL:0000210 denotes photoreceptor
T20188 32825-32834 GO:0010467 denotes expressed
T20189 32838-32852 CL:0000210 denotes photoreceptors
T20190 32864-32869 GO:0007567 denotes natal
T20191 32913-32917 PR:000014441 denotes mr-s
T20192 32980-32983 PR:000011432 denotes Nrl
T20193 32987-32992 NCBITaxon:10088 denotes mouse
T20194 33031-33041 GO:0010467 denotes expression
T20195 33068-33071 PR:000011432 denotes Nrl
T20196 33075-33082 UBERON:0000966 denotes retinas
T20197 33125-33129 PR:000014441 denotes mr-s
T20198 33169-33178 GO:0010467 denotes expressed
T20199 33179-33184 SO:0000704 denotes genes
T20200 33192-33195 PR:000011432 denotes Nrl
T20201 33199-33205 UBERON:0000966 denotes retina
T20202 33247-33253 SO:0000417 denotes domain
T20203 33287-33293 SO:0000417 denotes module
T20204 33303-33309 SO:0000417 denotes domain
T20205 33317-33321 PR:000014441 denotes mr-s
T20206 33367-33370 PR:000007229 denotes TEL
T20207 33382-33389 SO:0000417 denotes domains
T20208 33401-33408 SO:0001114 denotes helical
T20209 33423-33432 CHEBI:33839 denotes polymeric
T20210 33487-33496 GO:0000785 denotes chromatin
T20211 33552-33556 PR:000014441 denotes mr-s
T20212 33616-33620 PR:000014441 denotes mr-s
T20213 33748-33754 SO:0000417 denotes domain
T20214 33758-33762 PR:000014441 denotes mr-s
T20215 33792-33796 PR:000014441 denotes mr-s
T20216 33829-33835 SO:0000417 denotes domain
T20217 33910-33916 SO:0000417 denotes domain
T20218 33920-33924 PR:000014441 denotes mr-s
T20219 33948-33952 PR:000014441 denotes mr-s
T20220 33996-34002 SO:0000417 denotes domain
T20221 34006-34015 NCBITaxon:40674 denotes mammalian
T20222 34077-34083 SO:0000417 denotes domain
T20223 34087-34091 PR:000014441 denotes mr-s
T20224 34100-34109 CHEBI:33839 denotes polymeric
T20225 34175-34181 SO:0000417 denotes domain
T20226 34185-34189 PR:000014441 denotes mr-s
T20227 34204-34210 SO:0000417 denotes domain
T20228 34222-34231 CHEBI:36357 denotes molecules
T20229 34246-34250 PR:000014441 denotes mr-s
T20230 34273-34280 CHEBI:33839 denotes polymer
T20231 34293-34297 SO:0000704 denotes gene
T20232 34293-34307 GO:0016458 denotes gene silencing
T20233 34332-34341 GO:0032991 denotes complexes
T20234 34352-34361 GO:0000785 denotes chromatin
T20235 34383-34386 PR:000007229 denotes TEL
T20236 34500-34506 SO:0000417 denotes domain
T20237 34586-34593 CHEBI:33839 denotes polymer
T20238 34685-34689 PR:000014441 denotes mr-s
T20239 34716-34726 GO:0010467 denotes expression
T20240 34767-34771 PR:000014441 denotes mr-s
T20241 34780-34787 CHEBI:33839 denotes polymer
T20242 34845-34848 PR:000007229 denotes TEL
T20243 34890-34896 SO:0000417 denotes domain
T20244 34913-34919 SO:0000417 denotes domain
T20245 34961-34967 SO:0000417 denotes domain
T20246 34974-34977 PR:000007229 denotes TEL
T20247 35004-35011 CHEBI:33839 denotes polymer
T20248 35035-35050 GO:0051259 denotes oligomerization
T20249 35062-35068 SO:0000417 denotes domain
T20250 35085-35088 PR:000007229 denotes TEL
T20251 35325-35329 SO:0000704 denotes gene
T20252 35374-35383 PR:P05084 denotes Hunchback
T20253 35414-35420 SO:0000417 denotes domain
T20254 35486-35500 MOP:0000629 denotes polymerization
T20255 35513-35517 PR:000014441 denotes mr-s
T20256 35653-35657 PR:000014441 denotes mr-s
T20257 35784-35788 PR:000014441 denotes mr-s
T20258 35802-35806 PR:P04386 denotes GAL4
T20259 35819-35825 SO:0000417 denotes domain
T20260 35831-35834 PR:000014441 denotes mrs
T20261 35909-35913 PR:000014441 denotes mr-s
T20262 35940-35949 GO:0032991 denotes complexes
T20263 35971-35977 SO:0000417 denotes domain
T20264 36057-36061 PR:000014441 denotes mr-s
T20265 36078-36084 SO:0000417 denotes domain
T20266 36147-36161 MOP:0000629 denotes Polymerization
T20267 36173-36179 SO:0000417 denotes domain
T20268 36264-36267 PR:000007229 denotes TEL
T20269 36296-36301 NCBITaxon:9606 denotes human
T20270 36302-36333 PR:000009640 denotes lethal(3) malignant brain tumor
T20271 36322-36327 UBERON:0000955 denotes brain
T20272 36337-36344 PR:000009640 denotes L(3)MBT
T20273 36381-36387 SO:0000417 denotes domain
T20274 36481-36482 NCBITaxon:9606 denotes H
T20275 36483-36490 PR:000009640 denotes L(3)MBT
T20276 36555-36561 SO:0000417 denotes domain
T20277 36625-36629 PR:000014441 denotes mr-s
T20278 36862-36866 PR:000014441 denotes mr-s
T20279 36972-36978 SO:0000417 denotes domain
T20280 37028-37035 SO:0000155 denotes plasmid
T20281 37166-37170 PR:000014441 denotes mr-s
T20282 37232-37236 PR:000014441 denotes mr-s
T20283 37242-37251 NCBITaxon:7955 denotes zebrafish
T20284 37260-37265 NCBITaxon:9606 denotes human
T20285 37347-37360 GO:0031519 denotes PcG complexes
T20286 37362-37366 GO:0035102 denotes PRC1
T20287 37371-37375 GO:0035098 denotes PRC2
T20288 37399-37403 GO:0035098 denotes PRC2
T20289 37437-37446 GO:0016458 denotes silencing
T20290 37460-37467 PR:000043452 denotes histone
T20291 37460-37467 CHEBI:15358 denotes histone
T20292 37493-37500 PR:000043452 denotes histone
T20293 37493-37500 CHEBI:15358 denotes histone
T20294 37541-37548 CHEBI:15358 denotes histone
T20295 37541-37551 PR:000027594 denotes histone H3
T20296 37578-37586 GO:0016458 denotes silenced
T20297 37578-37596 GO:0035328 denotes silenced chromatin
T20298 37598-37602 GO:0035102 denotes PRC1
T20299 37633-37640 CHEBI:15358 denotes histone
T20300 37633-37643 PR:000027594 denotes histone H3
T20301 37666-37670 GO:0035098 denotes PRC2
T20302 37703-37707 SO:0000704 denotes gene
T20303 37728-37732 GO:0035102 denotes PRC1
T20304 37740-37749 GO:0000785 denotes chromatin
T20305 37740-37760 GO:0006338 denotes chromatin remodeling
T20306 37792-37807 GO:0016514 denotes SWI-SNF complex
T20307 37859-37863 GO:0035098 denotes PRC2
T20308 37933-37937 GO:0035102 denotes PRC1
T20309 38042-38045 PR:000014441 denotes mrs
T20310 38108-38122 CHEBI:46024 denotes trichostatin A
T20311 38226-38230 PR:000014441 denotes mr-s
T20312 38283-38295 GO:0035102 denotes PRC1 complex
T20313 38368-38372 PR:000014441 denotes mr-s
T20314 38475-38485 GO:0010467 denotes expression
T20315 38500-38505 SO:0000704 denotes genes
T20316 38513-38520 UBERON:0000966 denotes retinal
T20317 38513-38534 CL:0010009 denotes retinal photoreceptor
T20318 38521-38546 GO:0042461 denotes photoreceptor development
T20319 38603-38607 PR:000014441 denotes mr-s
T20320 38704-38708 PR:000014441 denotes mr-s
T22616 39772-39777 NCBITaxon:10088 denotes mouse
T22617 39778-39782 PR:000014441 denotes mr-s
T22618 39807-39816 GO:0010467 denotes expressed
T22619 39820-39827 UBERON:0000966 denotes retinal
T22620 39820-39842 CL:0010009 denotes retinal photoreceptors
T22621 39851-39863 UBERON:0001905 denotes pineal gland
T22622 39865-39869 PR:000014441 denotes mr-s
T22623 39903-39912 NCBITaxon:7955 denotes zebrafish
T22624 39924-39929 NCBITaxon:9606 denotes human
T22625 39964-39968 PR:000014441 denotes mr-s
T22626 39972-39985 CL:0000210 denotes photoreceptor
T22627 39972-39997 GO:0042461 denotes photoreceptor development
T22628 40029-40033 PR:000014441 denotes mr-s
T22629 40059-40066 GO:0005634 denotes nucleus
T22630 40114-40120 SO:0000417 denotes domain
T22631 40132-40136 PR:000014441 denotes mr-s
T22632 40154-40158 PR:P04386 denotes GAL4
T22633 40232-40236 PR:000014441 denotes mr-s
T22634 40310-40314 PR:000014441 denotes mr-s
T22635 40336-40344 CHEBI:36357 denotes molecule
T22636 40370-40384 _FRAGMENT denotes development of
T22637 40393-40407 GO:0042461 denotes photoreceptors
T22638 40412-40424 GO:0021982 denotes pineal gland
T22639 40385-40392 UBERON:0000966 denotes retinal
T22640 40385-40407 CL:0010009 denotes retinal photoreceptors
T22641 40412-40424 UBERON:0001905 denotes pineal gland
T23156 40449-40454 NCBITaxon:10088 denotes mouse
T23157 40455-40459 PR:000014441 denotes mr-s
T23158 40560-40565 NCBITaxon:10088 denotes mouse
T23159 40566-40571 SO:0000704 denotes genes
T23160 40572-40581 GO:0010467 denotes expressed
T23161 40604-40610 UBERON:0000966 denotes retina
T23162 40674-40679 NCBITaxon:10088 denotes mouse
T23163 40680-40687 UBERON:0000966 denotes retinal
T23164 40725-40728 NCBITaxon:10090 denotes Mm.
T23165 40748-40756 SO:0000857 denotes homology
T23166 40786-40791 SO:0000704 denotes genes
T23167 40799-40801 SO:0000028 denotes bp
T23168 40889-40894 NCBITaxon:10088 denotes mouse
T23169 40898-40905 UBERON:0000966 denotes retinal
T23170 40979-40992 GO:0097617 denotes hybridization
T23171 41006-41019 GO:0097617 denotes hybridization
T23172 41023-41028 NCBITaxon:10088 denotes mouse
T23173 41035-41042 UBERON:0000966 denotes retinal
T23174 41080-41085 NCBITaxon:10088 denotes mouse
T23175 41165-41169 PR:000014441 denotes mr-s
T23176 41330-41334 PR:000014441 denotes mr-s
T23177 41335-41339 SO:0000704 denotes gene
T23495 41434-41447 GO:0097617 denotes hybridization
T23496 41456-41458 SO:0000028 denotes bp
T23497 41476-41480 PR:000014441 denotes mr-s
T23498 41533-41546 GO:0097617 denotes hybridization
T23499 41556-41569 GO:0097617 denotes hybridization
T23774 41632-41644 GO:0009294 denotes transfection
T23775 41756-41762 NCBITaxon:27592 denotes bovine
T23776 41763-41768 UBERON:0001977 denotes serum
T23777 41788-41798 CHEBI:17334 denotes penicillin
T23778 41813-41825 CHEBI:17076 denotes streptomycin
T23779 41837-41849 GO:0009294 denotes transfection
T23780 41889-41906 CHEBI:77635 denotes calcium phosphate
T23781 41925-41937 GO:0009294 denotes transfection
T23782 41938-41945 CHEBI:33893 denotes reagent
T23783 42085-42103 CHEBI:32139 denotes sodium bicarbonate
T23784 42124-42136 GO:0009294 denotes transfection
T24134 42231-42238 UBERON:0000479 denotes tissues
T24135 42242-42247 UBERON:0007023 denotes adult
T24136 42248-42252 NCBITaxon:10088 denotes mice
T24137 42330-42337 CHEBI:2511 denotes agarose
T24138 42338-42350 CHEBI:16842 denotes formaldehyde
T24139 42376-42381 CHEBI:25614 denotes nylon
T24140 42425-42427 SO:0000028 denotes bp
T24141 42459-42465 SO:0000417 denotes domain
T24142 42469-42473 PR:000014441 denotes mr-s
T24143 42498-42511 GO:0097617 denotes hybridization
T24144 42513-42526 GO:0097617 denotes Hybridization
T24145 42673-42679 CHEBI:24866 denotes saline
T24146 42693-42715 CHEBI:8984 denotes sodium dodecyl sulfate
T24147 42717-42720 CHEBI:8984 denotes SDS
T24512 42773-42779 UBERON:0000479 denotes tissue
T24513 42818-42837 GO:0001171 denotes reverse transcribed
T24514 42879-42886 SO:0000112 denotes primers
T24515 42899-42906 SO:0000188 denotes introns
T24516 42925-42929 PR:000014441 denotes mr-s
T24517 43034-43036 SO:0000028 denotes bp
T24518 43097-43103 SO:0000112 denotes Primer
T24519 43114-43119 NCBITaxon:10088 denotes mouse
T24520 43212-43214 SO:0000028 denotes bp
T25067 43314-43318 PR:P04386 denotes GAL4
T25068 43390-43394 PR:P04386 denotes GAL4
T25069 43493-43497 PR:000014441 denotes mr-s
T25070 43514-43520 SO:0000440 denotes vector
T25071 43556-43561 NCBITaxon:10088 denotes mouse
T25072 43568-43575 UBERON:0000966 denotes retinal
T25073 43596-43602 SO:0000440 denotes vector
T25074 43729-43736 SO:0000155 denotes Plasmid
T25075 43781-43788 SO:0000155 denotes Plasmid
T25076 43893-43898 GO:0040007 denotes grown
T25077 43983-43988 CHEBI:75055 denotes X-gal
T25078 44006-44011 CHEBI:75055 denotes X-gal
T25079 44084-44088 PR:000014441 denotes mr-s
T25080 44191-44195 PR:000014441 denotes mr-s
T25081 44219-44226 SO:0000440 denotes vectors
T25082 44306-44311 CHEBI:75055 denotes X-gal
T26092 44446-44450 PR:000014441 denotes mr-s
T26093 44469-44472 PR:000014441 denotes mrs
T26094 44647-44657 GO:0010467 denotes expression
T26095 44658-44664 SO:0000440 denotes vector
T26096 44805-44809 PR:000014441 denotes mr-s
T26097 44814-44825 GO:0009294 denotes transfected
T26098 44853-44860 SO:0000155 denotes plasmid
T26099 44882-44899 CHEBI:77635 denotes calcium phosphate
T26100 44934-44946 GO:0009294 denotes transfection
T26101 45006-45010 CHEBI:9754 denotes Tris
T26102 45011-45014 CHEBI:17883 denotes HCl
T26103 45041-45046 CHEBI:6636 denotes MgCl2
T26104 45055-45059 CHEBI:26710 denotes NaCl
T26105 45066-45095 CHEBI:8102 denotes phenylmethylsulfonyl fluoride
T26106 45107-45119 CHEBI:78708 denotes Nonidet P-40
T26107 45125-45133 CHEBI:17754 denotes glycerol
T26108 45173-45181 CHEBI:60004 denotes cocktail
T26109 45273-45281 GO:0042571 denotes antibody
T26110 45581-45585 CHEBI:9754 denotes Tris
T26111 45586-45589 CHEBI:17883 denotes HCl
T26112 45607-45611 CHEBI:26710 denotes NaCl
T26113 45619-45624 CHEBI:6636 denotes MgCl2
T26114 45666-45669 CHEBI:8984 denotes SDS
T26115 45688-45691 CHEBI:8984 denotes SDS
T26116 45698-45702 CHEBI:9754 denotes Tris
T26117 45719-45722 CHEBI:18320 denotes DTT
T26118 45727-45735 CHEBI:17754 denotes glycerol
T26119 45743-45753 CHEBI:8682 denotes pyronine Y
T26120 45794-45816 CHEBI:8984 denotes sodium dodecyl sulfate
T26121 45853-45856 CHEBI:8984 denotes SDS
T26122 45886-45900 CHEBI:53325 denotes nitrocellulose
T26123 45985-45991 NCBITaxon:9986 denotes rabbit
T26124 46011-46019 GO:0042571 denotes antibody
T26125 46070-46081 NCBITaxon:3704 denotes horseradish
T26126 46094-46104 MOP:0000779 denotes conjugated
T26127 46105-46109 NCBITaxon:9925 denotes goat
T26128 46115-46121 NCBITaxon:9986 denotes rabbit
T26129 46122-46125 GO:0071735 denotes IgG
T26982 46223-46234 GO:0009294 denotes transfected
T26983 46239-46246 SO:0000155 denotes plasmid
T26984 46278-46282 PR:000014441 denotes mr-s
T26985 46363-46375 GO:0009294 denotes transfection
T26986 46547-46559 CHEBI:9750 denotes Triton X-100
T26987 46632-46638 NCBITaxon:27592 denotes bovine
T26988 46639-46644 UBERON:0001977 denotes serum
T26989 46639-46652 PR:000003918 denotes serum albumin
T26990 46723-46731 GO:0042571 denotes antibody
T26991 46862-46865 CHEBI:37987 denotes Cy3
T26992 46866-46876 MOP:0000779 denotes conjugated
T26993 46877-46881 NCBITaxon:9925 denotes goat
T26994 46882-46885 GO:0071735 denotes IgG
T26995 46894-46900 NCBITaxon:9986 denotes rabbit
T26996 46901-46904 GO:0071735 denotes IgG
T27780 47171-47179 SO:0000167 denotes promoter
T27781 47190-47194 PR:000014441 denotes mr-s
T27782 47222-47229 SO:0000155 denotes plasmid
T27783 47272-47279 SO:0001026 denotes genomic
T27784 47280-47291 _FRAGMENT denotes fragment of
T27785 47297-47301 SO:0000997 denotes gene
T27786 47292-47296 PR:000014441 denotes mr-s
T27787 47334-47341 SO:0000155 denotes plasmid
T27788 47357-47367 GO:0010467 denotes expression
T27789 47368-47375 SO:0000440 denotes vectors
T27790 47419-47422 PR:000005904 denotes Crx
T27791 47424-47428 PR:000012086 denotes Otx2
T27792 47430-47433 PR:000011432 denotes Nrl
T27793 47434-47439 SO:0000704 denotes genes
T27794 47452-47462 GO:0010467 denotes expression
T27795 47463-47469 SO:0000440 denotes vector
T27796 47533-47539 SO:0000440 denotes vector
T27797 47545-47548 PR:000005904 denotes Crx
T27798 47549-47561 SO:0000409 denotes binding site
T27799 47575-47577 SO:0000028 denotes bp
T27800 47649-47655 SO:0000440 denotes vector
T27801 47661-47664 PR:000005904 denotes Crx
T27802 47665-47677 SO:0000409 denotes binding site
T27803 47690-47692 SO:0000028 denotes bp
T27804 47763-47769 SO:0000440 denotes vector
T27805 47775-47778 PR:000005904 denotes Crx
T27806 47779-47791 SO:0000409 denotes binding site
T27807 47803-47805 SO:0000028 denotes bp
T27808 47878-47884 SO:0000440 denotes vector
T27809 47899-47902 PR:000005904 denotes Crx
T27810 47903-47916 SO:0000409 denotes binding sites
T27811 47960-47967 SO:0000155 denotes plasmid
T27812 48003-48007 PR:P04386 denotes GAL4
T27813 48068-48072 NCBITaxon:10633 denotes SV40
T27814 48081-48089 SO:0000167 denotes promoter
T27815 48186-48190 PR:000014441 denotes mr-s
T27816 48209-48217 CHEBI:35224 denotes effector
T27817 48218-48226 SO:0000155 denotes plasmids
T27818 48311-48317 SO:0000440 denotes vector
T27819 48396-48400 PR:P04386 denotes GAL4
T27820 48413-48419 SO:0000417 denotes domain
T27821 48450-48461 GO:0009294 denotes transfected
T27822 48481-48488 SO:0000155 denotes plasmid
T27823 48509-48519 GO:0010467 denotes expression
T27824 48520-48526 SO:0000440 denotes vector
T27825 48578-48590 GO:0009294 denotes transfection
T27826 48591-48598 CHEBI:33893 denotes reagent
T27827 48644-48656 GO:0009294 denotes transfection
T28636 9160-9164 PR:000014441 denotes mr-s
T28637 9206-9210 PR:000014441 denotes mr-s
T28638 9279-9285 SO:0000417 denotes domain
T28639 9335-9342 GO:0005634 denotes nuclear
T28640 9335-9362 SO:0001528 denotes nuclear localization signal
T28641 9399-9414 GO:0043631 denotes polyadenylation
T28642 9399-9433 SO:0000551 denotes polyadenylation termination signal
T28643 9456-9462 SO:0000417 denotes domain
T28644 9481-9487 SO:0000417 denotes domain
T28645 9518-9531 SO:0001117 denotes alpha helices
T28646 9773-9779 SO:0000417 denotes domain
T28647 10008-10013 NCBITaxon:10088 denotes mouse
T28648 10015-10018 NCBITaxon:10114 denotes rat
T28649 10020-10025 NCBITaxon:9606 denotes human
T28650 10037-10046 NCBITaxon:7955 denotes zebrafish
T28651 10047-10051 PR:000014441 denotes mr-s
T28652 10096-10103 SO:0000417 denotes domains
T28653 10158-10163 NCBITaxon:10088 denotes mouse
T28654 10219-10224 NCBITaxon:10088 denotes mouse
T29342 11423-11427 PR:000014441 denotes mr-s
T29343 11428-11438 GO:0010467 denotes expression
T29344 11453-11458 NCBITaxon:10088 denotes mouse
T29345 11459-11465 UBERON:0000966 denotes retina
T29346 11470-11482 UBERON:0001905 denotes pineal gland
T29347 11490-11494 PR:000014441 denotes mr-s
T29348 11495-11505 GO:0010467 denotes expression
T29349 11513-11527 _FRAGMENT denotes development of
T29350 11538-11544 GO:0060041 denotes retina
T29351 11532-11537 NCBITaxon:10088 denotes mouse
T29352 11538-11544 UBERON:0000966 denotes retina
T29353 11558-11571 GO:0097617 denotes hybridization
T29354 11582-11586 PR:000014441 denotes mr-s
T29355 11702-11706 PR:000014441 denotes mr-s
T29356 11736-11744 _FRAGMENT denotes layer of
T29357 11749-11755 UBERON:0001781 denotes retina
T29358 11803-11808 UBERON:0007023 denotes adult
T29359 11809-11815 UBERON:0000966 denotes retina
T29360 11859-11865 UBERON:0000966 denotes retina
T29361 11929-11933 PR:000014441 denotes mr-s
T29362 11934-11944 GO:0010467 denotes expression
T29363 11948-11953 UBERON:0007023 denotes adult
T29364 11954-11959 NCBITaxon:10088 denotes mouse
T29365 11960-11966 UBERON:0000062 denotes organs
T29366 11999-12003 PR:000014441 denotes mr-s
T29367 12004-12014 SO:0000673 denotes transcript
T29368 12075-12081 UBERON:0000922 denotes embryo
T29369 12086-12096 UBERON:0000468 denotes whole body
T29370 12113-12116 UBERON:0000970 denotes eye
T29371 12122-12128 UBERON:0000966 denotes retina
T29372 12133-12145 UBERON:0001905 denotes pineal gland
T29373 12150-12155 UBERON:0000955 denotes brain
T29374 12160-12165 UBERON:0002107 denotes liver
T29375 12167-12172 UBERON:0007023 denotes adult
T29376 12173-12179 UBERON:0000966 denotes retina
T29377 12181-12186 UBERON:0007023 denotes adult
T29378 12187-12199 UBERON:0001905 denotes pineal gland
T29379 12201-12206 UBERON:0007023 denotes adult
T29380 12207-12212 UBERON:0000955 denotes brain
T29381 12217-12222 UBERON:0007023 denotes adult
T29382 12223-12228 UBERON:0002107 denotes liver
T29383 12244-12247 UBERON:0001782 denotes RPE
T29384 12249-12275 UBERON:0001782 denotes retinal pigment epithelium
T29385 12257-12264 CHEBI:26130 denotes pigment
T29386 12277-12279 UBERON:0003902 denotes NR
T29387 12281-12294 UBERON:0003902 denotes neural retina
T29388 12301-12313 CL:0000031 denotes neuroblastic
T29389 12321-12324 UBERON:0001792 denotes GCL
T29390 12326-12339 CL:0000740 denotes ganglion cell
T29391 12326-12345 UBERON:0001792 denotes ganglion cell layer
T29392 12347-12350 UBERON:0001789 denotes ONL
T29393 12352-12371 UBERON:0001789 denotes outer nuclear layer
T29394 12358-12365 GO:0005634 denotes nuclear
T29395 12373-12376 UBERON:0001791 denotes INL
T29396 12378-12397 UBERON:0001791 denotes inner nuclear layer
T29397 12384-12391 GO:0005634 denotes nuclear
T30162 15019-15023 PR:000014441 denotes mr-s
T30163 15027-15036 GO:0065007 denotes regulated
T30164 15040-15043 PR:000005904 denotes Crx
T30165 15060-15073 GO:0097617 denotes hybridization
T30166 15092-15097 NCBITaxon:10088 denotes mouse
T30167 15098-15102 PR:000014441 denotes mr-s
T30168 15142-15145 PR:000005904 denotes Crx
T30169 15149-15156 UBERON:0000966 denotes retinas
T30170 15168-15172 PR:000014441 denotes mr-s
T30171 15173-15183 GO:0010467 denotes expression
T30172 15215-15218 PR:000005904 denotes Crx
T30173 15222-15228 UBERON:0000966 denotes retina
T30174 15306-15319 UBERON:0001905 denotes pineal glands
T30175 15340-15343 PR:000005904 denotes Crx
T30176 15347-15352 NCBITaxon:10088 denotes mouse
T30177 15385-15397 SO:0000006 denotes PCR products
T30178 15415-15421 SO:0000112 denotes primer
T30179 15441-15445 PR:000014441 denotes mr-s
T30180 15477-15482 CHEBI:15377 denotes Water
T30181 15527-15530 PR:000005904 denotes Crx
T30182 15546-15550 PR:000014441 denotes mr-s
T30183 15575-15583 SO:0000155 denotes plasmids
T30184 15641-15644 PR:000005904 denotes Crx
T30185 15645-15658 SO:0000409 denotes binding sites
T30186 15733-15744 GO:0009294 denotes transfected
T30187 15750-15753 PR:000005904 denotes Crx
T30188 15755-15759 PR:000012086 denotes Otx2
T30189 15761-15764 PR:000011432 denotes Nrl
T30190 15770-15773 PR:000005904 denotes Crx
T30191 15774-15777 PR:000011432 denotes Nrl
T30192 15883-15894 GO:0009294 denotes transfected
T30193 15904-15907 PR:000005904 denotes Crx
T30194 15908-15918 GO:0010467 denotes expression
T30195 15919-15925 SO:0000440 denotes vector
T30196 15927-15930 PR:000005904 denotes Crx
T30197 15946-15952 SO:0000440 denotes vector
T30198 15954-15957 PR:000005904 denotes Crx
T30850 20782-20786 PR:P04386 denotes GAL4
T30851 20810-20814 PR:000014441 denotes mr-s
T30852 20921-20925 PR:000014441 denotes mr-s
T30853 20973-20980 SO:0000417 denotes domains
T30854 21011-21015 PR:000014441 denotes mr-s
T30855 21050-21054 PR:P04386 denotes GAL4
T30856 21104-21111 SO:0000417 denotes domains
T30857 21132-21136 PR:000033987 denotes LacZ
T30858 21137-21147 GO:0010467 denotes expression
T30859 21342-21348 SO:0000417 denotes domain
T30860 21365-21371 SO:0000417 denotes domain
T30861 21391-21395 PR:000014441 denotes mr-s
T30862 21422-21426 PR:000014441 denotes mr-s
T30863 21476-21480 PR:000014441 denotes mr-s
T31372 22535-22539 PR:000014441 denotes mr-s
T31373 22570-22579 NCBITaxon:40674 denotes mammalian
T31374 22819-22825 SO:0000440 denotes vector
T31375 22881-22892 GO:0009294 denotes transfected
T31376 22962-22970 GO:0042571 denotes antibody
T31377 22995-23003 GO:0042571 denotes antibody
T31378 23092-23096 PR:000014441 denotes mr-s
T31379 23154-23165 GO:0009294 denotes transfected
T31380 23227-23235 GO:0042571 denotes antibody
T31381 23262-23270 GO:0042571 denotes antibody
T31709 25098-25102 PR:000014441 denotes mr-s
T31710 25106-25115 NCBITaxon:40674 denotes mammalian
T31711 25152-25156 PR:000014441 denotes mr-s
T31712 25161-25172 GO:0009294 denotes transfected
T31713 25214-25222 GO:0042571 denotes antibody
T31714 25293-25301 GO:0042571 denotes antibody
T31715 25307-25311 CHEBI:51231 denotes DAPI
T32368 29763-29767 PR:000014441 denotes mr-s
T32369 29777-29781 PR:P04386 denotes GAL4
T32370 29794-29800 SO:0000417 denotes domain
T32371 29952-29959 SO:0000155 denotes plasmid
T32372 29967-29978 GO:0009294 denotes transfected
T32373 30003-30011 CHEBI:35224 denotes effector
T32374 30012-30020 SO:0000155 denotes plasmids
T32375 30021-30031 GO:0010467 denotes expressing
T32376 30066-30070 PR:P04386 denotes GAL4
T32377 30108-30111 PR:000014441 denotes mrs
T32378 30128-30136 SO:0000155 denotes plasmids
T32379 30142-30153 GO:0009294 denotes transfected
T32380 30190-30197 SO:0000155 denotes plasmid
T32381 30251-30257 SO:0000440 denotes vector
T32382 30369-30380 GO:0009294 denotes transfected
T32383 30426-30433 SO:0000155 denotes plasmid
T32384 30528-30531 PR:000014441 denotes mrs
T32385 30622-30633 GO:0009294 denotes transfected
T32386 30660-30667 SO:0000155 denotes plasmid
T32387 30732-30735 PR:000014441 denotes mrs
T32388 30750-30761 GO:0009294 denotes transfected
T32389 30828-30835 SO:0000155 denotes plasmid
T32390 31073-31078 NCBITaxon:10088 denotes mouse
T32391 31080-31083 NCBITaxon:10114 denotes rat
T32392 31085-31090 NCBITaxon:9606 denotes human
T32393 31102-31111 NCBITaxon:7955 denotes zebrafish
T32394 31112-31116 PR:000014441 denotes mr-s
T7175 11389-11394 UBERON:0007023 denotes adult
T7176 11395-11401 UBERON:0000966 denotes retina
T7177 12400-12401 GO:0097617 denotes o
T7178 12407-12408 PR:000014441 denotes m
T7179 12416-12418 UBERON:0001781 denotes ti
T7166 11132-11149 GO:0007602 denotes phototransduction
T7167 11150-11155 SO:0000704 denotes genes
T7168 11207-11211 PR:000014441 denotes mr-s
T7169 11270-11289 UBERON:0001789 denotes outer nuclear layer
T7170 11276-11283 GO:0005634 denotes nuclear
T7171 11291-11294 UBERON:0001789 denotes ONL
T7172 11320-11330 GO:0010467 denotes expression
T7173 11334-11338 PR:000014441 denotes mr-s
T7174 11367-11381 CL:0000210 denotes photoreceptors
T7180 12420-12421 UBERON:0007023 denotes u
T7181 12421-12422 UBERON:0000966 denotes e
T7182 12429-12430 GO:0010467 denotes i
T7183 12431-12433 SO:0000673 denotes it
T7184 12439-12440 UBERON:0000922 denotes r
T7185 12440-12442 PR:000014441 denotes -s
T7186 12443-12453 GO:0010467 denotes expression
T7187 12459-12469 GO:0010467 denotes expression
T7188 12477-12481 PR:000014441 denotes mr-s
T7189 12482-12486 SO:0000704 denotes gene
T7190 12498-12503 UBERON:0007023 denotes adult
T7191 12504-12511 UBERON:0000479 denotes tissues
T7192 12537-12550 GO:0097617 denotes hybridization
T7193 12579-12586 UBERON:0000966 denotes retinal
T7194 12683-12689 UBERON:0000966 denotes retina
T7195 12787-12806 GO:0000380 denotes alternative spliced
T7196 12787-12818 SO:1001187 denotes alternative spliced transcripts
T7197 12857-12861 PR:000014441 denotes mr-s
T7198 12897-12902 UBERON:0007023 denotes adult
T7199 12903-12910 UBERON:0000479 denotes tissues
T7200 12943-12950 UBERON:0000479 denotes tissues
T7201 12958-12965 GO:0010467 denotes express
T7202 12966-12970 PR:000014441 denotes mr-s
T7203 13019-13025 UBERON:0000966 denotes retina
T7204 13063-13076 CL:0000210 denotes photoreceptor
T7205 13086-13091 SO:0000704 denotes genes
T7206 13101-13110 GO:0010467 denotes expressed
T7207 13118-13130 UBERON:0001905 denotes pineal gland
T7208 13153-13163 GO:0010467 denotes expression
T7209 13167-13171 PR:000014441 denotes mr-s
T9456 16043-16044 PR:000014441 denotes m
T9457 16044-16046 GO:0010467 denotes in
T9458 16051-16052 SO:0000006 denotes t
T9459 16054-16055 SO:0000112 denotes r
T9460 16058-16059 PR:000005904 denotes x
T9461 16062-16063 SO:0000409 denotes g
T9462 16064-16065 PR:000011432 denotes l
T9463 16073-16074 GO:0009294 denotes s
T9464 16076-16077 PR:000005904 denotes r
T9465 16160-16184 SO:0001668 denotes proximal promoter region
T9466 16188-16192 PR:000014441 denotes mr-s
T9467 16215-16219 SO:0000704 denotes gene
T9468 16272-16275 PR:000005904 denotes Crx
T9469 16277-16281 PR:000012086 denotes Otx2
T9470 16283-16286 PR:000011432 denotes Nrl
T9471 16287-16297 GO:0010467 denotes expression
T9472 16298-16305 SO:0000440 denotes vectors
T9473 16347-16351 PR:000014441 denotes mr-s
T9474 16385-16388 PR:000005904 denotes Crx
T9475 16397-16406 SO:0000993 denotes consensus
T9476 16502-16505 PR:000005904 denotes Crx
T9477 16509-16513 PR:000012086 denotes Otx2
T9525 18119-18132 SO:0000409 denotes binding sites
T9526 18136-18138 SO:0000028 denotes bp
T9527 18147-18149 SO:0000028 denotes bp
T9528 18168-18197 SO:0000315 denotes transcription initiation site
T9529 18225-18235 GO:0065007 denotes regulation
T9530 18239-18243 PR:000014441 denotes mr-s
T9531 18261-18264 PR:000005904 denotes Crx
T12196 18287-18291 PR:000014441 denotes mr-s
T12197 18304-18311 SO:0000417 denotes domains
T12198 18365-18372 SO:0000417 denotes modules
T12199 18395-18402 SO:0000417 denotes domains
T12200 18431-18437 SO:0000417 denotes domain
T12201 18488-18494 SO:0000417 denotes domain
T12202 18559-18565 SO:0000417 denotes domain
T12203 18573-18577 PR:000014441 denotes mr-s
T12204 18637-18643 SO:0000417 denotes module
T12265 23299-23303 PR:000014441 denotes mr-s
T12266 23351-23357 SO:0000417 denotes domain
T12267 23413-23419 SO:0000417 denotes domain
T12268 23423-23427 PR:000014441 denotes mr-s
T12269 23508-23514 SO:0000417 denotes domain
T12270 23647-23653 SO:0000417 denotes domain
T12271 23674-23692 PR:Q9VHA0 denotes Sex comb on midleg
T12272 23694-23697 PR:Q9VHA0 denotes Scm
T12273 23868-23872 PR:000014441 denotes mr-s
T12274 24045-24048 PR:000014441 denotes mrs
T3197 2255-2269 _FRAGMENT denotes development of
T3198 2284-2290 GO:0060041 denotes retina
T3199 2274-2283 NCBITaxon:40674 denotes mammalian
T3200 2284-2290 UBERON:0000966 denotes retina
T3201 2350-2361 _FRAGMENT denotes multipotent
T3202 2381-2386 CL:0000048 denotes cells
T3203 2362-2369 UBERON:0000966 denotes retinal
T3204 2362-2386 CL:0002672 denotes retinal progenitor cells
T3205 2412-2426 CL:0000210 denotes photoreceptors
T3206 2468-2474 UBERON:0000966 denotes retina
T3207 2479-2490 NCBITaxon:7742 denotes vertebrates
T3208 2517-2531 CL:0000210 denotes photoreceptors
T3209 2533-2537 CL:0000604 denotes rods
T3210 2542-2547 CL:0000573 denotes cones
T3211 2549-2553 CL:0000604 denotes Rods
T3212 2586-2591 CL:0000573 denotes cones
T3213 2647-2653 GO:0007601 denotes vision
T3214 2655-2672 GO:0007602 denotes Phototransduction
T3215 2727-2733 CHEBI:30212 denotes photon
T3216 2764-2780 GO:0016037 denotes capture of light
T3217 2786-2800 CHEBI:16066 denotes 11-cis-retinal
T3218 2804-2815 CHEBI:23240 denotes chromophore
T3219 2829-2834 PR:000001119 denotes opsin
T3220 2845-2854 PR:000001245 denotes rhodopsin
T20321 38739-38752 GO:0097617 denotes hybridization
T20322 38777-38781 PR:000014441 denotes mr-s
T20323 38782-38792 GO:0010467 denotes expression
T20324 38812-38819 UBERON:0000966 denotes retinal
T20325 38812-38834 CL:0010009 denotes retinal photoreceptors
T20326 38900-38905 SO:0000704 denotes genes
T20327 38909-38913 PR:000014441 denotes mr-s
T20328 38927-38940 CL:0000210 denotes photoreceptor
T20329 38941-38946 SO:0000704 denotes genes
T20330 38962-38966 PR:000014441 denotes mr-s
T20331 38984-38994 GO:0010467 denotes expression
T20332 39002-39015 CL:0000210 denotes photoreceptor
T20333 39016-39021 SO:0000704 denotes genes
T20334 39025-39028 _FRAGMENT denotes rod
T20335 39038-39052 CL:0000604 denotes photoreceptors
T20336 39033-39052 CL:0000573 denotes cone photoreceptors
T20337 39092-39096 PR:000014441 denotes mr-s
T20338 39112-39138 _FRAGMENT denotes cell fate determination of
T20339 39143-39157 GO:0043703 denotes photoreceptors
T20340 39165-39184 GO:0042671 denotes cone photoreceptors
T20341 39139-39157 CL:0000604 denotes rod photoreceptors
T20342 39165-39184 CL:0000573 denotes cone photoreceptors
T20343 39192-39211 CL:0000573 denotes cone photoreceptors
T20344 39232-39247 UBERON:0019248 denotes early embryonic
T20345 39258-39263 NCBITaxon:10088 denotes mouse
T20346 39264-39277 GO:0060041 denotes retinogenesis
T20347 39279-39297 CL:0000604 denotes rod photoreceptors
T20348 39324-39338 _FRAGMENT denotes late embryonic
T20349 39359-39365 UBERON:0007220 denotes period
T20350 39353-39358 GO:0007567 denotes natal
T20351 39376-39386 GO:0010467 denotes expression
T20352 39398-39402 PR:000014441 denotes mr-s
T20353 39420-39424 PR:000014441 denotes mr-s
T20354 39428-39437 GO:0010467 denotes expressed
T20355 39441-39459 CL:0000604 denotes rod photoreceptors
T20356 39471-39490 CL:0000573 denotes cone photoreceptors
T20357 39502-39507 PR:000011403 denotes Nr2e3
T20358 39587-39605 CL:0000573 denotes cone photoreceptor
T20359 39615-39620 SO:0000704 denotes genes
T20360 39660-39664 PR:000014441 denotes mr-s
T20361 39678-39682 NCBITaxon:10088 denotes mice
T20362 39712-39716 PR:000014441 denotes mr-s
R4870 T20349 T20348 _lexicallyChainedTo period,late embryonic
R6423 T22637 T22636 _lexicallyChainedTo photoreceptors,development of
R6424 T22638 T22636 _lexicallyChainedTo pineal gland,development of
R7868 T27785 T27784 _lexicallyChainedTo gene,fragment of
R8347 T29350 T29349 _lexicallyChainedTo retina,development of
R8348 T29357 T29356 _lexicallyChainedTo retina,layer of
R392 T3198 T3197 _lexicallyChainedTo retina,development of
R393 T3202 T3201 _lexicallyChainedTo cells,multipotent
R394 T3250 T3249 _lexicallyChainedTo of photoreceptors,cell fate determination
R395 T3314 T3313 _lexicallyChainedTo genes,silencing of
R1700 T7219 T7218 _lexicallyChainedTo for genes,fragments
R3804 T13804 T13803 _lexicallyChainedTo cells,nucleus of
R4867 T20335 T20334 _lexicallyChainedTo photoreceptors,rod
R4868 T20339 T20338 _lexicallyChainedTo photoreceptors,cell fate determination of
R4869 T20340 T20338 _lexicallyChainedTo cone photoreceptors,cell fate determination of

craft-sa-dev

Id Subject Object Predicate Lexical cue
T1004 0-120 sentence denotes Cloning and characterization of mr-s, a novel SAM domain protein, predominantly expressed in retinal photoreceptor cells
T1005 8-11 CC denotes and
T1006 12-28 NN denotes characterization
T1007 29-31 IN denotes of
T1008 32-34 NN denotes mr
T1009 35-36 NN denotes s
T1010 34-35 HYPH denotes -
T1011 36-38 , denotes ,
T1012 38-39 DT denotes a
T1013 57-64 NN denotes protein
T1014 40-45 JJ denotes novel
T1015 46-49 NN denotes SAM
T1016 50-56 NN denotes domain
T1017 64-66 , denotes ,
T1018 66-79 RB denotes predominantly
T1019 80-89 VBN denotes expressed
T1020 90-92 IN denotes in
T1021 93-100 JJ denotes retinal
T1022 115-120 NNS denotes cells
T1023 101-114 NN denotes photoreceptor
T1024 120-121 sentence denotes
T1025 131-141 sentence denotes Background
T1026 131-141 NN denotes Background
T1027 141-267 sentence denotes Sterile alpha motif (SAM) domains are ~70 residues long and have been reported as common protein-protein interaction modules.
T1028 142-149 JJ denotes Sterile
T1029 156-161 NN denotes motif
T1030 150-155 NN denotes alpha
T1031 168-175 NNS denotes domains
T1032 162-163 -LRB- denotes (
T1033 163-166 NN denotes SAM
T1034 166-167 -RRB- denotes )
T1035 176-179 VBP denotes are
T1036 180-181 SYM denotes ~
T1037 181-183 CD denotes 70
T1038 184-192 NNS denotes residues
T1039 193-197 JJ denotes long
T1040 198-201 CC denotes and
T1041 202-206 VBP denotes have
T1042 212-220 VBN denotes reported
T1043 207-211 VBN denotes been
T1044 221-223 IN denotes as
T1045 224-230 JJ denotes common
T1046 259-266 NNS denotes modules
T1047 231-238 NN denotes protein
T1048 239-246 NN denotes protein
T1049 238-239 HYPH denotes -
T1050 247-258 NN denotes interaction
T1051 266-267 . denotes .
T1052 267-397 sentence denotes This domain is found in a large number of proteins, including Polycomb group (PcG) proteins and ETS family transcription factors.
T1053 268-272 DT denotes This
T1054 273-279 NN denotes domain
T1055 283-288 VBN denotes found
T1056 280-282 VBZ denotes is
T1057 289-291 IN denotes in
T1058 292-293 DT denotes a
T1059 300-306 NN denotes number
T1060 294-299 JJ denotes large
T1061 307-309 IN denotes of
T1062 310-318 NN denotes proteins
T1063 318-320 , denotes ,
T1064 320-329 VBG denotes including
T1065 330-338 NN denotes Polycomb
T1066 339-344 NN denotes group
T1067 351-359 NN denotes proteins
T1068 345-346 -LRB- denotes (
T1069 346-349 NN denotes PcG
T1070 349-350 -RRB- denotes )
T1071 360-363 CC denotes and
T1072 364-367 NN denotes ETS
T1073 368-374 NN denotes family
T1074 389-396 NNS denotes factors
T1075 375-388 NN denotes transcription
T1076 396-397 . denotes .
T1077 397-652 sentence denotes In this work, we report the cloning and functional characterization of a novel SAM domain-containing protein, which is predominantly expressed in retinal photoreceptors and the pineal gland and is designated mouse mr-s (major retinal SAM domain protein).
T1078 398-400 IN denotes In
T1079 415-421 VBP denotes report
T1080 401-405 DT denotes this
T1081 406-410 NN denotes work
T1082 410-412 , denotes ,
T1083 412-414 PRP denotes we
T1084 422-425 DT denotes the
T1085 426-433 NN denotes cloning
T1086 434-437 CC denotes and
T1087 438-448 JJ denotes functional
T1088 449-465 NN denotes characterization
T1089 466-468 IN denotes of
T1090 469-470 DT denotes a
T1091 499-506 NN denotes protein
T1092 471-476 JJ denotes novel
T1093 477-480 NN denotes SAM
T1094 481-487 NN denotes domain
T1095 488-498 VBG denotes containing
T1096 487-488 HYPH denotes -
T1097 506-508 , denotes ,
T1098 508-513 WDT denotes which
T1099 531-540 VBN denotes expressed
T1100 514-516 VBZ denotes is
T1101 517-530 RB denotes predominantly
T1102 541-543 IN denotes in
T1103 544-551 JJ denotes retinal
T1104 552-566 NNS denotes photoreceptors
T1105 567-570 CC denotes and
T1106 571-574 DT denotes the
T1107 582-587 NN denotes gland
T1108 575-581 JJ denotes pineal
T1109 588-591 CC denotes and
T1110 592-594 VBZ denotes is
T1111 595-605 VBN denotes designated
T1112 606-611 NN denotes mouse
T1113 615-616 NN denotes s
T1114 612-614 NN denotes mr
T1115 614-615 HYPH denotes -
T1116 617-618 -LRB- denotes (
T1117 643-650 NN denotes protein
T1118 618-623 JJ denotes major
T1119 624-631 JJ denotes retinal
T1120 632-635 NN denotes SAM
T1121 636-642 NN denotes domain
T1122 650-651 -RRB- denotes )
T1123 651-652 . denotes .
T1124 652-661 sentence denotes Results
T1125 654-661 NNS denotes Results
T1126 661-817 sentence denotes mr-s is evolutionarily conserved from zebrafish through human, organisms through which the mechanism of photoreceptor development is also highly conserved.
T1127 662-664 NN denotes mr
T1128 665-666 NN denotes s
T1129 664-665 HYPH denotes -
T1130 685-694 VBN denotes conserved
T1131 667-669 VBZ denotes is
T1132 670-684 RB denotes evolutionarily
T1133 695-699 IN denotes from
T1134 700-709 NN denotes zebrafish
T1135 710-717 IN denotes through
T1136 718-723 JJ denotes human
T1137 723-725 , denotes ,
T1138 725-734 NNS denotes organisms
T1139 735-742 IN denotes through
T1140 807-816 VBN denotes conserved
T1141 743-748 WDT denotes which
T1142 749-752 DT denotes the
T1143 753-762 NN denotes mechanism
T1144 763-765 IN denotes of
T1145 766-779 NN denotes photoreceptor
T1146 780-791 NN denotes development
T1147 792-794 VBZ denotes is
T1148 795-799 RB denotes also
T1149 800-806 RB denotes highly
T1150 816-817 . denotes .
T1151 817-1029 sentence denotes Phylogenetic analysis suggests that the SAM domain of mr-s is most closely related to a mouse polyhomeotic (ph) ortholog, Mph1/Rae28, which is known as an epigenetic molecule involved in chromatin modifications.
T1152 818-830 JJ denotes Phylogenetic
T1153 831-839 NN denotes analysis
T1154 840-848 VBZ denotes suggests
T1155 849-853 IN denotes that
T1156 893-900 VBN denotes related
T1157 854-857 DT denotes the
T1158 862-868 NN denotes domain
T1159 858-861 NN denotes SAM
T1160 869-871 IN denotes of
T1161 872-874 NN denotes mr
T1162 875-876 NN denotes s
T1163 874-875 HYPH denotes -
T1164 877-879 VBZ denotes is
T1165 880-884 RBS denotes most
T1166 885-892 RB denotes closely
T1167 901-903 IN denotes to
T1168 904-905 DT denotes a
T1169 930-938 NN denotes ortholog
T1170 906-911 NN denotes mouse
T1171 912-924 NN denotes polyhomeotic
T1172 925-926 -LRB- denotes (
T1173 926-928 NN denotes ph
T1174 928-929 -RRB- denotes )
T1175 938-940 , denotes ,
T1176 940-944 NN denotes Mph1
T1177 945-950 NN denotes Rae28
T1178 944-945 HYPH denotes /
T1179 950-952 , denotes ,
T1180 952-957 WDT denotes which
T1181 961-966 VBN denotes known
T1182 958-960 VBZ denotes is
T1183 967-969 IN denotes as
T1184 970-972 DT denotes an
T1185 984-992 NN denotes molecule
T1186 973-983 JJ denotes epigenetic
T1187 993-1001 VBN denotes involved
T1188 1002-1004 IN denotes in
T1189 1005-1014 NN denotes chromatin
T1190 1015-1028 NNS denotes modifications
T1191 1028-1029 . denotes .
T1192 1029-1163 sentence denotes These findings provide the possibility that mr-s may play a critical role by regulating gene expression in photoreceptor development.
T1193 1030-1035 DT denotes These
T1194 1036-1044 NNS denotes findings
T1195 1045-1052 VBP denotes provide
T1196 1053-1056 DT denotes the
T1197 1057-1068 NN denotes possibility
T1198 1069-1073 IN denotes that
T1199 1083-1087 VB denotes play
T1200 1074-1076 NN denotes mr
T1201 1077-1078 NN denotes s
T1202 1076-1077 HYPH denotes -
T1203 1079-1082 MD denotes may
T1204 1088-1089 DT denotes a
T1205 1099-1103 NN denotes role
T1206 1090-1098 JJ denotes critical
T1207 1104-1106 IN denotes by
T1208 1107-1117 VBG denotes regulating
T1209 1118-1122 NN denotes gene
T1210 1123-1133 NN denotes expression
T1211 1134-1136 IN denotes in
T1212 1137-1150 NN denotes photoreceptor
T1213 1151-1162 NN denotes development
T1214 1162-1163 . denotes .
T1215 1163-1332 sentence denotes mr-s is preferentially expressed in the photoreceptors at postnatal day 3–6 (P3-6), when photoreceptors undergo terminal differentiation, and in the adult pineal gland.
T1216 1164-1166 NN denotes mr
T1217 1167-1168 NN denotes s
T1218 1166-1167 HYPH denotes -
T1219 1187-1196 VBN denotes expressed
T1220 1169-1171 VBZ denotes is
T1221 1172-1186 RB denotes preferentially
T1222 1197-1199 IN denotes in
T1223 1200-1203 DT denotes the
T1224 1204-1218 NNS denotes photoreceptors
T1225 1219-1221 IN denotes at
T1226 1222-1231 JJ denotes postnatal
T1227 1236-1237 CD denotes 3
T1228 1232-1235 NN denotes day
T1229 1237-1238 SYM denotes –
T1230 1238-1239 CD denotes 6
T1231 1240-1241 -LRB- denotes (
T1232 1241-1243 NN denotes P3
T1233 1243-1244 SYM denotes -
T1234 1244-1245 CD denotes 6
T1235 1245-1246 -RRB- denotes )
T1236 1246-1248 , denotes ,
T1237 1248-1252 WRB denotes when
T1238 1268-1275 VBP denotes undergo
T1239 1253-1267 NNS denotes photoreceptors
T1240 1276-1284 JJ denotes terminal
T1241 1285-1300 NN denotes differentiation
T1242 1300-1302 , denotes ,
T1243 1302-1305 CC denotes and
T1244 1306-1308 IN denotes in
T1245 1309-1312 DT denotes the
T1246 1326-1331 NN denotes gland
T1247 1313-1318 JJ denotes adult
T1248 1319-1325 JJ denotes pineal
T1249 1331-1332 . denotes .
T1250 1332-1417 sentence denotes Transcription of mr-s is directly regulated by the cone-rod homeodomain protein Crx.
T1251 1333-1346 NN denotes Transcription
T1252 1367-1376 VBN denotes regulated
T1253 1347-1349 IN denotes of
T1254 1350-1352 NN denotes mr
T1255 1353-1354 NN denotes s
T1256 1352-1353 HYPH denotes -
T1257 1355-1357 VBZ denotes is
T1258 1358-1366 RB denotes directly
T1259 1377-1379 IN denotes by
T1260 1380-1383 DT denotes the
T1261 1405-1412 NN denotes protein
T1262 1384-1388 NN denotes cone
T1263 1389-1392 NN denotes rod
T1264 1388-1389 HYPH denotes -
T1265 1393-1404 NN denotes homeodomain
T1266 1413-1416 NN denotes Crx
T1267 1416-1417 . denotes .
T1268 1417-1551 sentence denotes Immunoprecipitation assay showed that the mr-s protein self-associates mainly through the SAM domain-containing region as well as ph.
T1269 1418-1437 NN denotes Immunoprecipitation
T1270 1438-1443 NN denotes assay
T1271 1444-1450 VBD denotes showed
T1272 1451-1455 IN denotes that
T1273 1478-1488 VBZ denotes associates
T1274 1456-1459 DT denotes the
T1275 1465-1472 NN denotes protein
T1276 1460-1462 NN denotes mr
T1277 1463-1464 NN denotes s
T1278 1462-1463 HYPH denotes -
T1279 1473-1477 NN denotes self
T1280 1477-1478 HYPH denotes -
T1281 1489-1495 RB denotes mainly
T1282 1496-1503 IN denotes through
T1283 1504-1507 DT denotes the
T1284 1530-1536 NN denotes region
T1285 1508-1511 NN denotes SAM
T1286 1512-1518 NN denotes domain
T1287 1519-1529 VBG denotes containing
T1288 1518-1519 HYPH denotes -
T1289 1537-1539 RB denotes as
T1290 1545-1547 IN denotes as
T1291 1540-1544 RB denotes well
T1292 1548-1550 NN denotes ph
T1293 1550-1551 . denotes .
T1294 1551-1646 sentence denotes The mr-s protein localizes mainly in the nucleus, when mr-s is overexpressed in HEK293T cells.
T1295 1552-1555 DT denotes The
T1296 1561-1568 NN denotes protein
T1297 1556-1558 NN denotes mr
T1298 1559-1560 NN denotes s
T1299 1558-1559 HYPH denotes -
T1300 1569-1578 VBZ denotes localizes
T1301 1579-1585 RB denotes mainly
T1302 1586-1588 IN denotes in
T1303 1589-1592 DT denotes the
T1304 1593-1600 NN denotes nucleus
T1305 1600-1602 , denotes ,
T1306 1602-1606 WRB denotes when
T1307 1615-1628 VBN denotes overexpressed
T1308 1607-1609 NN denotes mr
T1309 1610-1611 NN denotes s
T1310 1609-1610 HYPH denotes -
T1311 1612-1614 VBZ denotes is
T1312 1629-1631 IN denotes in
T1313 1632-1639 NN denotes HEK293T
T1314 1640-1645 NNS denotes cells
T1315 1645-1646 . denotes .
T1316 1646-1784 sentence denotes Moreover, in the luciferase assays, we found that mr-s protein fused to GAL4 DNA-binding domain functions as a transcriptional repressor.
T1317 1647-1655 RB denotes Moreover
T1318 1686-1691 VBD denotes found
T1319 1655-1657 , denotes ,
T1320 1657-1659 IN denotes in
T1321 1660-1663 DT denotes the
T1322 1675-1681 NNS denotes assays
T1323 1664-1674 NN denotes luciferase
T1324 1681-1683 , denotes ,
T1325 1683-1685 PRP denotes we
T1326 1692-1696 IN denotes that
T1327 1743-1752 VBZ denotes functions
T1328 1697-1699 NN denotes mr
T1329 1700-1701 NN denotes s
T1330 1699-1700 HYPH denotes -
T1331 1702-1709 NN denotes protein
T1332 1710-1715 VBN denotes fused
T1333 1716-1718 IN denotes to
T1334 1719-1723 NN denotes GAL4
T1335 1736-1742 NN denotes domain
T1336 1724-1727 NN denotes DNA
T1337 1728-1735 VBG denotes binding
T1338 1727-1728 HYPH denotes -
T1339 1753-1755 IN denotes as
T1340 1756-1757 DT denotes a
T1341 1774-1783 NN denotes repressor
T1342 1758-1773 JJ denotes transcriptional
T1343 1783-1784 . denotes .
T1344 1784-1925 sentence denotes We revealed that the repression activity of mr-s is not due to a homophilic interaction through its SAM domain but to the C-terminal region.
T1345 1785-1787 PRP denotes We
T1346 1788-1796 VBD denotes revealed
T1347 1797-1801 IN denotes that
T1348 1834-1836 VBZ denotes is
T1349 1802-1805 DT denotes the
T1350 1817-1825 NN denotes activity
T1351 1806-1816 NN denotes repression
T1352 1826-1828 IN denotes of
T1353 1829-1831 NN denotes mr
T1354 1832-1833 NN denotes s
T1355 1831-1832 HYPH denotes -
T1356 1837-1840 RB denotes not
T1357 1841-1844 IN denotes due
T1358 1845-1847 IN denotes to
T1359 1848-1849 DT denotes a
T1360 1861-1872 NN denotes interaction
T1361 1850-1860 JJ denotes homophilic
T1362 1873-1880 IN denotes through
T1363 1881-1884 PRP$ denotes its
T1364 1889-1895 NN denotes domain
T1365 1885-1888 NN denotes SAM
T1366 1896-1899 CC denotes but
T1367 1900-1902 IN denotes to
T1368 1903-1906 DT denotes the
T1369 1918-1924 NN denotes region
T1370 1907-1908 NN denotes C
T1371 1909-1917 JJ denotes terminal
T1372 1908-1909 HYPH denotes -
T1373 1924-1925 . denotes .
T1374 1925-1937 sentence denotes Conclusion
T1375 1927-1937 NN denotes Conclusion
T1376 1937-2048 sentence denotes We identified a novel gene, mr-s, which is predominantly expressed in retinal photoreceptors and pineal gland.
T1377 1938-1940 PRP denotes We
T1378 1941-1951 VBD denotes identified
T1379 1952-1953 DT denotes a
T1380 1960-1964 NN denotes gene
T1381 1954-1959 JJ denotes novel
T1382 1964-1966 , denotes ,
T1383 1966-1968 NN denotes mr
T1384 1969-1970 NN denotes s
T1385 1968-1969 HYPH denotes -
T1386 1970-1972 , denotes ,
T1387 1972-1977 WDT denotes which
T1388 1995-2004 VBN denotes expressed
T1389 1978-1980 VBZ denotes is
T1390 1981-1994 RB denotes predominantly
T1391 2005-2007 IN denotes in
T1392 2008-2015 JJ denotes retinal
T1393 2016-2030 NNS denotes photoreceptors
T1394 2031-2034 CC denotes and
T1395 2035-2041 JJ denotes pineal
T1396 2042-2047 NN denotes gland
T1397 2047-2048 . denotes .
T1398 2048-2235 sentence denotes Based on its expression pattern and biochemical analysis, we predict that mr-s may function as a transcriptional repressor in photoreceptor cells and in pinealocytes of the pineal gland.
T1399 2049-2054 VBN denotes Based
T1400 2110-2117 VBP denotes predict
T1401 2055-2057 IN denotes on
T1402 2058-2061 PRP$ denotes its
T1403 2073-2080 NN denotes pattern
T1404 2062-2072 NN denotes expression
T1405 2081-2084 CC denotes and
T1406 2085-2096 JJ denotes biochemical
T1407 2097-2105 NN denotes analysis
T1408 2105-2107 , denotes ,
T1409 2107-2109 PRP denotes we
T1410 2118-2122 IN denotes that
T1411 2132-2140 VB denotes function
T1412 2123-2125 NN denotes mr
T1413 2126-2127 NN denotes s
T1414 2125-2126 HYPH denotes -
T1415 2128-2131 MD denotes may
T1416 2141-2143 IN denotes as
T1417 2144-2145 DT denotes a
T1418 2162-2171 NN denotes repressor
T1419 2146-2161 JJ denotes transcriptional
T1420 2172-2174 IN denotes in
T1421 2175-2188 NN denotes photoreceptor
T1422 2189-2194 NNS denotes cells
T1423 2195-2198 CC denotes and
T1424 2199-2201 IN denotes in
T1425 2202-2214 NNS denotes pinealocytes
T1426 2215-2217 IN denotes of
T1427 2218-2221 DT denotes the
T1428 2229-2234 NN denotes gland
T1429 2222-2228 JJ denotes pineal
T1430 2234-2235 . denotes .
T3611 2342-2346 NN denotes pool
T3612 2347-2349 IN denotes of
T3613 2350-2361 JJ denotes multipotent
T3614 2381-2386 NNS denotes cells
T3615 2362-2369 JJ denotes retinal
T3616 2370-2380 NN denotes progenitor
T3617 2386-2387 . denotes .
T3618 2387-2475 sentence denotes Among these cell types, photoreceptors account for over 70% of all cells in the retina.
T3619 2388-2393 IN denotes Among
T3620 2427-2434 VBP denotes account
T3621 2394-2399 DT denotes these
T3622 2405-2410 NNS denotes types
T3623 2400-2404 NN denotes cell
T3624 2410-2412 , denotes ,
T3625 2412-2426 NNS denotes photoreceptors
T3626 2435-2438 IN denotes for
T3627 2439-2443 IN denotes over
T3628 2444-2446 CD denotes 70
T3629 2446-2447 NN denotes %
T3630 2448-2450 IN denotes of
T3631 2451-2454 DT denotes all
T3632 2455-2460 NNS denotes cells
T3633 2461-2463 IN denotes in
T3634 2464-2467 DT denotes the
T3635 2468-2474 NN denotes retina
T3636 2474-2475 . denotes .
T3637 2475-2548 sentence denotes In vertebrates, there are two classes of photoreceptors, rods and cones.
T3638 2476-2478 IN denotes In
T3639 2498-2501 VBP denotes are
T3640 2479-2490 NNS denotes vertebrates
T3641 2490-2492 , denotes ,
T3642 2492-2497 EX denotes there
T3643 2502-2505 CD denotes two
T3644 2506-2513 NNS denotes classes
T3645 2514-2516 IN denotes of
T3646 2517-2531 NNS denotes photoreceptors
T3647 2531-2533 , denotes ,
T3648 2533-2537 NNS denotes rods
T3649 2538-2541 CC denotes and
T3650 2542-2547 NNS denotes cones
T3651 2547-2548 . denotes .
T3652 2548-2654 sentence denotes Rods are sensors of dim light, while cones function in bright light and are responsible for color vision.
T3653 2549-2553 NNS denotes Rods
T3654 2554-2557 VBP denotes are
T3655 2558-2565 NNS denotes sensors
T3656 2566-2568 IN denotes of
T3657 2569-2572 JJ denotes dim
T3658 2573-2578 NN denotes light
T3659 2578-2580 , denotes ,
T3660 2580-2585 IN denotes while
T3661 2592-2600 VBP denotes function
T3662 2586-2591 NNS denotes cones
T3663 2601-2603 IN denotes in
T3664 2604-2610 JJ denotes bright
T3665 2611-2616 NN denotes light
T3666 2617-2620 CC denotes and
T3667 2621-2624 VBP denotes are
T3668 2625-2636 JJ denotes responsible
T3669 2637-2640 IN denotes for
T3670 2641-2646 NN denotes color
T3671 2647-2653 NN denotes vision
T3672 2653-2654 . denotes .
T3673 2654-2888 sentence denotes Phototransduction, a series of signal amplifications detecting a single photon of light, is initiated by the capture of light with 11-cis-retinal, a chromophore bound by the opsin proteins: rhodopsin in rods and cone opsins in cones.
T3674 2655-2672 NN denotes Phototransduction
T3675 2747-2756 VBN denotes initiated
T3676 2672-2674 , denotes ,
T3677 2674-2675 DT denotes a
T3678 2676-2682 NN denotes series
T3679 2683-2685 IN denotes of
T3680 2686-2692 NN denotes signal
T3681 2693-2707 NNS denotes amplifications
T3682 2708-2717 VBG denotes detecting
T3683 2718-2719 DT denotes a
T3684 2727-2733 NN denotes photon
T3685 2720-2726 JJ denotes single
T3686 2734-2736 IN denotes of
T3687 2737-2742 NN denotes light
T3688 2742-2744 , denotes ,
T3689 2744-2746 VBZ denotes is
T3690 2757-2759 IN denotes by
T3691 2760-2763 DT denotes the
T3692 2764-2771 NN denotes capture
T3693 2772-2774 IN denotes of
T3694 2775-2780 NN denotes light
T3695 2781-2785 IN denotes with
T3696 2786-2788 CD denotes 11
T3697 2789-2792 NN denotes cis
T3698 2788-2789 HYPH denotes -
T3699 2792-2793 HYPH denotes -
T3700 2793-2800 JJ denotes retinal
T3701 2800-2802 , denotes ,
T3702 2802-2803 DT denotes a
T3703 2804-2815 NN denotes chromophore
T3704 2816-2821 VBN denotes bound
T3705 2822-2824 IN denotes by
T3706 2825-2828 DT denotes the
T3707 2835-2843 NN denotes proteins
T3708 2829-2834 NN denotes opsin
T3709 2843-2845 : denotes :
T3710 2845-2854 NN denotes rhodopsin
T3711 2855-2857 IN denotes in
T3712 2858-2862 NNS denotes rods
T3713 2863-2866 CC denotes and
T3714 2867-2871 NN denotes cone
T3715 2872-2878 NNS denotes opsins
T3716 2879-2881 IN denotes in
T3717 2882-2887 NNS denotes cones
T3718 2887-2888 . denotes .
T3719 2888-3026 sentence denotes The proteins that carry out phototransduction are located in an elaborate and highly specialized membranous structure, the outer segment.
T3720 2889-2892 DT denotes The
T3721 2893-2901 NN denotes proteins
T3722 2939-2946 VBN denotes located
T3723 2902-2906 WDT denotes that
T3724 2907-2912 VBP denotes carry
T3725 2913-2916 RP denotes out
T3726 2917-2934 NN denotes phototransduction
T3727 2935-2938 VBP denotes are
T3728 2947-2949 IN denotes in
T3729 2950-2952 DT denotes an
T3730 2997-3006 NN denotes structure
T3731 2953-2962 JJ denotes elaborate
T3732 2963-2966 CC denotes and
T3733 2967-2973 RB denotes highly
T3734 2974-2985 JJ denotes specialized
T3735 2986-2996 JJ denotes membranous
T3736 3006-3008 , denotes ,
T3737 3008-3011 DT denotes the
T3738 3018-3025 NN denotes segment
T3739 3012-3017 JJ denotes outer
T3740 3025-3026 . denotes .
T3741 3026-3155 sentence denotes The outer segment appears to be relatively fragile, degenerating in response to many environmental and/or genetic perturbations.
T3742 3027-3030 DT denotes The
T3743 3037-3044 NN denotes segment
T3744 3031-3036 JJ denotes outer
T3745 3045-3052 VBZ denotes appears
T3746 3053-3055 TO denotes to
T3747 3056-3058 VB denotes be
T3748 3059-3069 RB denotes relatively
T3749 3070-3077 JJ denotes fragile
T3750 3077-3079 , denotes ,
T3751 3079-3091 VBG denotes degenerating
T3752 3092-3094 IN denotes in
T3753 3095-3103 NN denotes response
T3754 3104-3106 IN denotes to
T3755 3107-3111 JJ denotes many
T3756 3141-3154 NNS denotes perturbations
T3757 3112-3125 JJ denotes environmental
T3758 3126-3129 CC denotes and
T3759 3129-3130 HYPH denotes /
T3760 3130-3132 CC denotes or
T3761 3133-3140 JJ denotes genetic
T3762 3154-3155 . denotes .
T3763 3155-3271 sentence denotes In the rodent retina, the production of specific cell types during development progresses in a general order [1,2].
T3764 3156-3158 IN denotes In
T3765 3235-3245 VBZ denotes progresses
T3766 3159-3162 DT denotes the
T3767 3170-3176 NN denotes retina
T3768 3163-3169 NN denotes rodent
T3769 3176-3178 , denotes ,
T3770 3178-3181 DT denotes the
T3771 3182-3192 NN denotes production
T3772 3193-3195 IN denotes of
T3773 3196-3204 JJ denotes specific
T3774 3210-3215 NNS denotes types
T3775 3205-3209 NN denotes cell
T3776 3216-3222 IN denotes during
T3777 3223-3234 NN denotes development
T3778 3246-3248 IN denotes in
T3779 3249-3250 DT denotes a
T3780 3259-3264 NN denotes order
T3781 3251-3258 JJ denotes general
T3782 3265-3266 -LRB- denotes [
T3783 3268-3269 CD denotes 2
T3784 3266-3267 CD denotes 1
T3785 3267-3268 , denotes ,
T3786 3269-3270 -RRB- denotes ]
T3787 3270-3271 . denotes .
T3788 3271-3332 sentence denotes Rod photoreceptor generation peaks around the time of birth.
T3789 3272-3275 NN denotes Rod
T3790 3276-3289 NN denotes photoreceptor
T3791 3290-3300 NN denotes generation
T3792 3301-3306 VBZ denotes peaks
T3793 3307-3313 IN denotes around
T3794 3314-3317 DT denotes the
T3795 3318-3322 NN denotes time
T3796 3323-3325 IN denotes of
T3797 3326-3331 NN denotes birth
T3798 3331-3332 . denotes .
T3799 3332-3485 sentence denotes Cone photoreceptors, ganglion cells, horizontal cells and amacrine cells are generated earlier, while Müller glia and bipolar cells are generated later.
T3800 3333-3337 NN denotes Cone
T3801 3338-3352 NNS denotes photoreceptors
T3802 3410-3419 VBN denotes generated
T3803 3352-3354 , denotes ,
T3804 3354-3362 NN denotes ganglion
T3805 3363-3368 NNS denotes cells
T3806 3368-3370 , denotes ,
T3807 3370-3380 JJ denotes horizontal
T3808 3381-3386 NNS denotes cells
T3809 3387-3390 CC denotes and
T3810 3391-3399 NN denotes amacrine
T3811 3400-3405 NNS denotes cells
T3812 3406-3409 VBP denotes are
T3813 3420-3427 RBR denotes earlier
T3814 3427-3429 , denotes ,
T3815 3429-3434 IN denotes while
T3816 3469-3478 VBN denotes generated
T3817 3435-3441 NNP denotes Müller
T3818 3442-3446 NNS denotes glia
T3819 3447-3450 CC denotes and
T3820 3451-3458 JJ denotes bipolar
T3821 3459-3464 NNS denotes cells
T3822 3465-3468 VBP denotes are
T3823 3479-3484 RB denotes later
T3824 3484-3485 . denotes .
T3825 3485-3684 sentence denotes This production of different cell types at different times appears to derive from differences in the intrinsic properties of progenitor cells involved in the transcription or chromatin modification.
T3826 3486-3490 DT denotes This
T3827 3491-3501 NN denotes production
T3828 3545-3552 VBZ denotes appears
T3829 3502-3504 IN denotes of
T3830 3505-3514 JJ denotes different
T3831 3520-3525 NNS denotes types
T3832 3515-3519 NN denotes cell
T3833 3526-3528 IN denotes at
T3834 3529-3538 JJ denotes different
T3835 3539-3544 NNS denotes times
T3836 3553-3555 TO denotes to
T3837 3556-3562 VB denotes derive
T3838 3563-3567 IN denotes from
T3839 3568-3579 NNS denotes differences
T3840 3580-3582 IN denotes in
T3841 3583-3586 DT denotes the
T3842 3597-3607 NNS denotes properties
T3843 3587-3596 JJ denotes intrinsic
T3844 3608-3610 IN denotes of
T3845 3611-3621 NN denotes progenitor
T3846 3622-3627 NNS denotes cells
T3847 3628-3636 VBN denotes involved
T3848 3637-3639 IN denotes in
T3849 3640-3643 DT denotes the
T3850 3644-3657 NN denotes transcription
T3851 3658-3660 CC denotes or
T3852 3661-3670 NN denotes chromatin
T3853 3671-3683 NN denotes modification
T3854 3683-3684 . denotes .
T3855 3684-3786 sentence denotes Recent studies identified several important transcription factors of photoreceptor development [3-7].
T3856 3685-3691 JJ denotes Recent
T3857 3692-3699 NNS denotes studies
T3858 3700-3710 VBD denotes identified
T3859 3711-3718 JJ denotes several
T3860 3743-3750 NNS denotes factors
T3861 3719-3728 JJ denotes important
T3862 3729-3742 NN denotes transcription
T3863 3751-3753 IN denotes of
T3864 3754-3767 NN denotes photoreceptor
T3865 3768-3779 NN denotes development
T3866 3780-3781 -LRB- denotes [
T3867 3781-3782 CD denotes 3
T3868 3782-3783 SYM denotes -
T3869 3783-3784 CD denotes 7
T3870 3784-3785 -RRB- denotes ]
T3871 3785-3786 . denotes .
T3872 3786-3941 sentence denotes Two Otx family homeobox genes, Otx2 and Crx, play essential roles in early cell fate determination and terminal differentiation of photoreceptors [3,8,9].
T3873 3787-3790 CD denotes Two
T3874 3811-3816 NNS denotes genes
T3875 3791-3794 NN denotes Otx
T3876 3795-3801 NN denotes family
T3877 3802-3810 NN denotes homeobox
T3878 3832-3836 VBP denotes play
T3879 3816-3818 , denotes ,
T3880 3818-3822 NN denotes Otx2
T3881 3823-3826 CC denotes and
T3882 3827-3830 NN denotes Crx
T3883 3830-3832 , denotes ,
T3884 3837-3846 JJ denotes essential
T3885 3847-3852 NNS denotes roles
T3886 3853-3855 IN denotes in
T3887 3856-3861 JJ denotes early
T3888 3872-3885 NN denotes determination
T3889 3862-3866 NN denotes cell
T3890 3867-3871 NN denotes fate
T3891 3886-3889 CC denotes and
T3892 3890-3898 JJ denotes terminal
T3893 3899-3914 NN denotes differentiation
T3894 3915-3917 IN denotes of
T3895 3918-3932 NNS denotes photoreceptors
T3896 3933-3934 -LRB- denotes [
T3897 3938-3939 CD denotes 9
T3898 3934-3935 CD denotes 3
T3899 3935-3936 , denotes ,
T3900 3936-3937 CD denotes 8
T3901 3937-3938 , denotes ,
T3902 3939-3940 -RRB- denotes ]
T3903 3940-3941 . denotes .
T3904 3941-4054 sentence denotes In the absence of Otx2 function, differentiating photoreceptor cells are converted to amacrine-like neurons [9].
T3905 3942-3944 IN denotes In
T3906 4015-4024 VBN denotes converted
T3907 3945-3948 DT denotes the
T3908 3949-3956 NN denotes absence
T3909 3957-3959 IN denotes of
T3910 3960-3964 NN denotes Otx2
T3911 3965-3973 NN denotes function
T3912 3973-3975 , denotes ,
T3913 3975-3990 VBG denotes differentiating
T3914 4005-4010 NNS denotes cells
T3915 3991-4004 NN denotes photoreceptor
T3916 4011-4014 VBP denotes are
T3917 4025-4027 IN denotes to
T3918 4028-4036 NN denotes amacrine
T3919 4037-4041 JJ denotes like
T3920 4036-4037 HYPH denotes -
T3921 4042-4049 NNS denotes neurons
T3922 4050-4051 -LRB- denotes [
T3923 4051-4052 CD denotes 9
T3924 4052-4053 -RRB- denotes ]
T3925 4053-4054 . denotes .
T3926 4054-4273 sentence denotes Crx, a downstream target of Otx2, controls the transcription of various photoreceptor cell-specific genes and is essential for the formation of outer segments, synaptic terminals, and phototransduction pathways [8,10].
T3927 4055-4058 NN denotes Crx
T3928 4089-4097 VBZ denotes controls
T3929 4058-4060 , denotes ,
T3930 4060-4061 DT denotes a
T3931 4073-4079 NN denotes target
T3932 4062-4072 JJ denotes downstream
T3933 4080-4082 IN denotes of
T3934 4083-4087 NN denotes Otx2
T3935 4087-4089 , denotes ,
T3936 4098-4101 DT denotes the
T3937 4102-4115 NN denotes transcription
T3938 4116-4118 IN denotes of
T3939 4119-4126 JJ denotes various
T3940 4155-4160 NNS denotes genes
T3941 4127-4140 NN denotes photoreceptor
T3942 4141-4145 NN denotes cell
T3943 4146-4154 JJ denotes specific
T3944 4145-4146 HYPH denotes -
T3945 4161-4164 CC denotes and
T3946 4165-4167 VBZ denotes is
T3947 4168-4177 JJ denotes essential
T3948 4178-4181 IN denotes for
T3949 4182-4185 DT denotes the
T3950 4186-4195 NN denotes formation
T3951 4196-4198 IN denotes of
T3952 4199-4204 JJ denotes outer
T3953 4205-4213 NNS denotes segments
T3954 4213-4215 , denotes ,
T3955 4215-4223 JJ denotes synaptic
T3956 4224-4233 NNS denotes terminals
T3957 4233-4235 , denotes ,
T3958 4235-4238 CC denotes and
T3959 4239-4256 NN denotes phototransduction
T3960 4257-4265 NNS denotes pathways
T3961 4266-4267 -LRB- denotes [
T3962 4269-4271 CD denotes 10
T3963 4267-4268 CD denotes 8
T3964 4268-4269 , denotes ,
T3965 4271-4272 -RRB- denotes ]
T3966 4272-4273 . denotes .
T3967 4273-4509 sentence denotes Crx transcripts begin to be expressed in developing photoreceptors at embryonic day 12.5 (E12.5) in the mouse and a strong upregulation of Crx transcription is apparent across the differentiating photoreceptors at postnatal day 6 (P6).
T3968 4274-4277 NN denotes Crx
T3969 4278-4289 NNS denotes transcripts
T3970 4290-4295 VBP denotes begin
T3971 4296-4298 TO denotes to
T3972 4302-4311 VBN denotes expressed
T3973 4299-4301 VB denotes be
T3974 4312-4314 IN denotes in
T3975 4315-4325 VBG denotes developing
T3976 4326-4340 NNS denotes photoreceptors
T3977 4341-4343 IN denotes at
T3978 4344-4353 JJ denotes embryonic
T3979 4354-4357 NN denotes day
T3980 4358-4362 CD denotes 12.5
T3981 4363-4364 -LRB- denotes (
T3982 4364-4369 NN denotes E12.5
T3983 4369-4370 -RRB- denotes )
T3984 4371-4373 IN denotes in
T3985 4374-4377 DT denotes the
T3986 4378-4383 NN denotes mouse
T3987 4384-4387 CC denotes and
T3988 4388-4389 DT denotes a
T3989 4397-4409 NN denotes upregulation
T3990 4390-4396 JJ denotes strong
T3991 4431-4433 VBZ denotes is
T3992 4410-4412 IN denotes of
T3993 4413-4416 NN denotes Crx
T3994 4417-4430 NN denotes transcription
T3995 4434-4442 JJ denotes apparent
T3996 4443-4449 IN denotes across
T3997 4450-4453 DT denotes the
T3998 4470-4484 NNS denotes photoreceptors
T3999 4454-4469 VBG denotes differentiating
T4000 4485-4487 IN denotes at
T4001 4488-4497 JJ denotes postnatal
T4002 4498-4501 NN denotes day
T4003 4502-4503 CD denotes 6
T4004 4504-4505 -LRB- denotes (
T4005 4505-4507 NN denotes P6
T4006 4507-4508 -RRB- denotes )
T4007 4508-4509 . denotes .
T4008 4509-4727 sentence denotes Photoreceptor cells in the Crx knockout (KO) mice exhibit a dramatic reduction of many photoreceptor molecules including visual pigments and develop neither photoreceptor outer segments nor a synaptic terminus [8,10].
T4009 4510-4523 NN denotes Photoreceptor
T4010 4524-4529 NNS denotes cells
T4011 4560-4567 VBP denotes exhibit
T4012 4530-4532 IN denotes in
T4013 4533-4536 DT denotes the
T4014 4555-4559 NNS denotes mice
T4015 4537-4540 NN denotes Crx
T4016 4541-4549 NN denotes knockout
T4017 4550-4551 -LRB- denotes (
T4018 4551-4553 NN denotes KO
T4019 4553-4554 -RRB- denotes )
T4020 4568-4569 DT denotes a
T4021 4579-4588 NN denotes reduction
T4022 4570-4578 JJ denotes dramatic
T4023 4589-4591 IN denotes of
T4024 4592-4596 JJ denotes many
T4025 4611-4620 NNS denotes molecules
T4026 4597-4610 NN denotes photoreceptor
T4027 4621-4630 VBG denotes including
T4028 4631-4637 JJ denotes visual
T4029 4638-4646 NNS denotes pigments
T4030 4647-4650 CC denotes and
T4031 4651-4658 VBP denotes develop
T4032 4659-4666 CC denotes neither
T4033 4687-4695 NNS denotes segments
T4034 4667-4680 NN denotes photoreceptor
T4035 4681-4686 JJ denotes outer
T4036 4696-4699 CC denotes nor
T4037 4700-4701 DT denotes a
T4038 4711-4719 NN denotes terminus
T4039 4702-4710 JJ denotes synaptic
T4040 4720-4721 -LRB- denotes [
T4041 4723-4725 CD denotes 10
T4042 4721-4722 CD denotes 8
T4043 4722-4723 , denotes ,
T4044 4725-4726 -RRB- denotes ]
T4045 4726-4727 . denotes .
T4046 4727-4982 sentence denotes In addition, mutations of human CRX have been demonstrated to be associated with three types of photoreceptor diseases: autosomal dominant cone-rod dystrophy 2, autosomal dominant-type retinitis pigmentosa, and Leber's congenital amaurosis (LCA) [11-14].
T4047 4728-4730 IN denotes In
T4048 4774-4786 VBN denotes demonstrated
T4049 4731-4739 NN denotes addition
T4050 4739-4741 , denotes ,
T4051 4741-4750 NNS denotes mutations
T4052 4751-4753 IN denotes of
T4053 4754-4759 JJ denotes human
T4054 4760-4763 NN denotes CRX
T4055 4764-4768 VBP denotes have
T4056 4769-4773 VBN denotes been
T4057 4787-4789 TO denotes to
T4058 4793-4803 VBN denotes associated
T4059 4790-4792 VB denotes be
T4060 4804-4808 IN denotes with
T4061 4809-4814 CD denotes three
T4062 4815-4820 NNS denotes types
T4063 4821-4823 IN denotes of
T4064 4824-4837 NN denotes photoreceptor
T4065 4838-4846 NNS denotes diseases
T4066 4846-4848 : denotes :
T4067 4848-4857 JJ denotes autosomal
T4068 4858-4866 JJ denotes dominant
T4069 4876-4885 NN denotes dystrophy
T4070 4867-4871 NN denotes cone
T4071 4872-4875 NN denotes rod
T4072 4871-4872 HYPH denotes -
T4073 4886-4887 CD denotes 2
T4074 4887-4889 , denotes ,
T4075 4889-4898 JJ denotes autosomal
T4076 4899-4907 JJ denotes dominant
T4077 4908-4912 NN denotes type
T4078 4907-4908 HYPH denotes -
T4079 4923-4933 NN denotes pigmentosa
T4080 4913-4922 NN denotes retinitis
T4081 4933-4935 , denotes ,
T4082 4935-4938 CC denotes and
T4083 4939-4944 NNP denotes Leber
T4084 4958-4967 NN denotes amaurosis
T4085 4944-4946 POS denotes 's
T4086 4947-4957 JJ denotes congenital
T4087 4968-4969 -LRB- denotes (
T4088 4969-4972 NN denotes LCA
T4089 4972-4973 -RRB- denotes )
T4090 4974-4975 -LRB- denotes [
T4091 4975-4977 CD denotes 11
T4092 4977-4978 SYM denotes -
T4093 4978-4980 CD denotes 14
T4094 4980-4981 -RRB- denotes ]
T4095 4981-4982 . denotes .
T4096 4982-5164 sentence denotes While Otx2 and Crx control general photoreceptor development, three other transcription factors, TRβ2, Nrl, and Nr2e3 regulate the specification of photoreceptor cell types [4,5,7].
T4097 4983-4988 IN denotes While
T4098 5002-5009 VBP denotes control
T4099 4989-4993 NN denotes Otx2
T4100 4994-4997 CC denotes and
T4101 4998-5001 NN denotes Crx
T4102 5101-5109 VBP denotes regulate
T4103 5010-5017 JJ denotes general
T4104 5032-5043 NN denotes development
T4105 5018-5031 NN denotes photoreceptor
T4106 5043-5045 , denotes ,
T4107 5045-5050 CD denotes three
T4108 5071-5078 NNS denotes factors
T4109 5051-5056 JJ denotes other
T4110 5057-5070 NN denotes transcription
T4111 5078-5080 , denotes ,
T4112 5080-5084 NN denotes TRβ2
T4113 5084-5086 , denotes ,
T4114 5086-5089 NN denotes Nrl
T4115 5089-5091 , denotes ,
T4116 5091-5094 CC denotes and
T4117 5095-5100 NN denotes Nr2e3
T4118 5110-5113 DT denotes the
T4119 5114-5127 NN denotes specification
T4120 5128-5130 IN denotes of
T4121 5131-5144 NN denotes photoreceptor
T4122 5150-5155 NNS denotes types
T4123 5145-5149 NN denotes cell
T4124 5156-5157 -LRB- denotes [
T4125 5161-5162 CD denotes 7
T4126 5157-5158 CD denotes 4
T4127 5158-5159 , denotes ,
T4128 5159-5160 CD denotes 5
T4129 5160-5161 , denotes ,
T4130 5162-5163 -RRB- denotes ]
T4131 5163-5164 . denotes .
T4132 5164-5321 sentence denotes SAM domains (also known as Pointed, HLH, or SPM domains) are ~70 residues long and have been reported as common protein-protein interaction modules [15-17].
T4133 5165-5168 NN denotes SAM
T4134 5169-5176 NNS denotes domains
T4135 5222-5225 VBP denotes are
T4136 5177-5178 -LRB- denotes (
T4137 5178-5182 RB denotes also
T4138 5183-5188 VBN denotes known
T4139 5189-5191 IN denotes as
T4140 5192-5199 JJ denotes Pointed
T4141 5213-5220 NNS denotes domains
T4142 5199-5201 , denotes ,
T4143 5201-5204 NN denotes HLH
T4144 5204-5206 , denotes ,
T4145 5206-5208 CC denotes or
T4146 5209-5212 NN denotes SPM
T4147 5220-5221 -RRB- denotes )
T4148 5226-5227 SYM denotes ~
T4149 5227-5229 CD denotes 70
T4150 5230-5238 NNS denotes residues
T4151 5239-5243 JJ denotes long
T4152 5244-5247 CC denotes and
T4153 5248-5252 VBP denotes have
T4154 5258-5266 VBN denotes reported
T4155 5253-5257 VBN denotes been
T4156 5267-5269 IN denotes as
T4157 5270-5276 JJ denotes common
T4158 5305-5312 NNS denotes modules
T4159 5277-5284 NN denotes protein
T4160 5285-5292 NN denotes protein
T4161 5284-5285 HYPH denotes -
T4162 5293-5304 NN denotes interaction
T4163 5313-5314 -LRB- denotes [
T4164 5314-5316 CD denotes 15
T4165 5316-5317 SYM denotes -
T4166 5317-5319 CD denotes 17
T4167 5319-5320 -RRB- denotes ]
T4168 5320-5321 . denotes .
T4169 5321-5683 sentence denotes This domain is found in a large number of proteins, including Polycomb group (PcG) proteins [18], serine threonine kinases [19], Eph family receptor tyrosine kinases [20], the p73 tumor suppressor [21], the RNA-binding protein Smaug [22], diacylglycerol kinases [23,24], yeast mating type signaling proteins [19,25] and ETS family transcription factors [26,27].
T4170 5322-5326 DT denotes This
T4171 5327-5333 NN denotes domain
T4172 5337-5342 VBN denotes found
T4173 5334-5336 VBZ denotes is
T4174 5343-5345 IN denotes in
T4175 5346-5347 DT denotes a
T4176 5354-5360 NN denotes number
T4177 5348-5353 JJ denotes large
T4178 5361-5363 IN denotes of
T4179 5364-5372 NN denotes proteins
T4180 5372-5374 , denotes ,
T4181 5374-5383 VBG denotes including
T4182 5384-5392 NN denotes Polycomb
T4183 5393-5398 NN denotes group
T4184 5405-5413 NN denotes proteins
T4185 5399-5400 -LRB- denotes (
T4186 5400-5403 NN denotes PcG
T4187 5403-5404 -RRB- denotes )
T4188 5414-5415 -LRB- denotes [
T4189 5415-5417 CD denotes 18
T4190 5417-5418 -RRB- denotes ]
T4191 5418-5420 , denotes ,
T4192 5420-5426 NN denotes serine
T4193 5437-5444 NNS denotes kinases
T4194 5427-5436 NN denotes threonine
T4195 5445-5446 -LRB- denotes [
T4196 5446-5448 CD denotes 19
T4197 5448-5449 -RRB- denotes ]
T4198 5449-5451 , denotes ,
T4199 5451-5454 NN denotes Eph
T4200 5455-5461 NN denotes family
T4201 5480-5487 NNS denotes kinases
T4202 5462-5470 NN denotes receptor
T4203 5471-5479 NN denotes tyrosine
T4204 5488-5489 -LRB- denotes [
T4205 5489-5491 CD denotes 20
T4206 5491-5492 -RRB- denotes ]
T4207 5492-5494 , denotes ,
T4208 5494-5497 DT denotes the
T4209 5508-5518 NN denotes suppressor
T4210 5498-5501 NN denotes p73
T4211 5502-5507 NN denotes tumor
T4212 5519-5520 -LRB- denotes [
T4213 5520-5522 CD denotes 21
T4214 5522-5523 -RRB- denotes ]
T4215 5523-5525 , denotes ,
T4216 5525-5528 DT denotes the
T4217 5541-5548 NN denotes protein
T4218 5529-5532 NN denotes RNA
T4219 5533-5540 VBG denotes binding
T4220 5532-5533 HYPH denotes -
T4221 5549-5554 NN denotes Smaug
T4222 5555-5556 -LRB- denotes [
T4223 5556-5558 CD denotes 22
T4224 5558-5559 -RRB- denotes ]
T4225 5559-5561 , denotes ,
T4226 5561-5575 NN denotes diacylglycerol
T4227 5576-5583 NNS denotes kinases
T4228 5584-5585 -LRB- denotes [
T4229 5588-5590 CD denotes 24
T4230 5585-5587 CD denotes 23
T4231 5587-5588 , denotes ,
T4232 5590-5591 -RRB- denotes ]
T4233 5591-5593 , denotes ,
T4234 5593-5598 NN denotes yeast
T4235 5606-5610 NN denotes type
T4236 5599-5605 NN denotes mating
T4237 5621-5629 NN denotes proteins
T4238 5611-5620 NN denotes signaling
T4239 5630-5631 -LRB- denotes [
T4240 5634-5636 CD denotes 25
T4241 5631-5633 CD denotes 19
T4242 5633-5634 , denotes ,
T4243 5636-5637 -RRB- denotes ]
T4244 5638-5641 CC denotes and
T4245 5642-5645 NN denotes ETS
T4246 5646-5652 NN denotes family
T4247 5667-5674 NNS denotes factors
T4248 5653-5666 NN denotes transcription
T4249 5675-5676 -LRB- denotes [
T4250 5679-5681 CD denotes 27
T4251 5676-5678 CD denotes 26
T4252 5678-5679 , denotes ,
T4253 5681-5682 -RRB- denotes ]
T4254 5682-5683 . denotes .
T4255 5683-5788 sentence denotes The PcG proteins are transcriptional repressors that maintain gene silencing during development [28-30].
T4256 5684-5687 DT denotes The
T4257 5692-5700 NN denotes proteins
T4258 5688-5691 NN denotes PcG
T4259 5701-5704 VBP denotes are
T4260 5705-5720 JJ denotes transcriptional
T4261 5721-5731 NNS denotes repressors
T4262 5732-5736 WDT denotes that
T4263 5737-5745 VBP denotes maintain
T4264 5746-5750 NN denotes gene
T4265 5751-5760 NN denotes silencing
T4266 5761-5767 IN denotes during
T4267 5768-5779 NN denotes development
T4268 5780-5781 -LRB- denotes [
T4269 5781-5783 CD denotes 28
T4270 5783-5784 SYM denotes -
T4271 5784-5786 CD denotes 30
T4272 5786-5787 -RRB- denotes ]
T4273 5787-5788 . denotes .
T4274 5788-5857 sentence denotes In mammals, PcG proteins are also implicated in Hox gene regulation.
T4275 5789-5791 IN denotes In
T4276 5823-5833 VBN denotes implicated
T4277 5792-5799 NNS denotes mammals
T4278 5799-5801 , denotes ,
T4279 5801-5804 NN denotes PcG
T4280 5805-5813 NN denotes proteins
T4281 5814-5817 VBP denotes are
T4282 5818-5822 RB denotes also
T4283 5834-5836 IN denotes in
T4284 5837-5840 NN denotes Hox
T4285 5841-5845 NN denotes gene
T4286 5846-5856 NN denotes regulation
T4287 5856-5857 . denotes .
T4288 5857-5967 sentence denotes Their biological activity lies in stable silencing of specific sets of genes through chromatin modifications.
T4289 5858-5863 PRP$ denotes Their
T4290 5875-5883 NN denotes activity
T4291 5864-5874 JJ denotes biological
T4292 5884-5888 VBZ denotes lies
T4293 5889-5891 IN denotes in
T4294 5892-5898 JJ denotes stable
T4295 5899-5908 NN denotes silencing
T4296 5909-5911 IN denotes of
T4297 5912-5920 JJ denotes specific
T4298 5921-5925 NNS denotes sets
T4299 5926-5928 IN denotes of
T4300 5929-5934 NNS denotes genes
T4301 5935-5942 IN denotes through
T4302 5943-5952 NN denotes chromatin
T4303 5953-5966 NNS denotes modifications
T4304 5966-5967 . denotes .
T4305 5967-6156 sentence denotes A member of polycomb repressive complex 1 (PRC1), ph, contains a SAM domain at the C-terminal, and PRC1 complex is known to form helical, head-to-tail polymers through its SAM domain [31].
T4306 5968-5969 DT denotes A
T4307 5970-5976 NN denotes member
T4308 6022-6030 VBZ denotes contains
T4309 5977-5979 IN denotes of
T4310 5980-5988 NN denotes polycomb
T4311 6000-6007 NN denotes complex
T4312 5989-5999 JJ denotes repressive
T4313 6008-6009 CD denotes 1
T4314 6010-6011 -LRB- denotes (
T4315 6011-6015 NN denotes PRC1
T4316 6015-6016 -RRB- denotes )
T4317 6016-6018 , denotes ,
T4318 6018-6020 NN denotes ph
T4319 6020-6022 , denotes ,
T4320 6031-6032 DT denotes a
T4321 6037-6043 NN denotes domain
T4322 6033-6036 NN denotes SAM
T4323 6044-6046 IN denotes at
T4324 6047-6050 DT denotes the
T4325 6053-6061 NN denotes terminal
T4326 6051-6052 NN denotes C
T4327 6052-6053 HYPH denotes -
T4328 6061-6063 , denotes ,
T4329 6063-6066 CC denotes and
T4330 6067-6071 NN denotes PRC1
T4331 6072-6079 NN denotes complex
T4332 6083-6088 VBN denotes known
T4333 6080-6082 VBZ denotes is
T4334 6089-6091 TO denotes to
T4335 6092-6096 VB denotes form
T4336 6097-6104 JJ denotes helical
T4337 6119-6127 NNS denotes polymers
T4338 6104-6106 , denotes ,
T4339 6106-6110 NN denotes head
T4340 6110-6111 HYPH denotes -
T4341 6111-6113 IN denotes to
T4342 6113-6114 HYPH denotes -
T4343 6114-6118 NN denotes tail
T4344 6128-6135 IN denotes through
T4345 6136-6139 PRP$ denotes its
T4346 6144-6150 NN denotes domain
T4347 6140-6143 NN denotes SAM
T4348 6151-6152 -LRB- denotes [
T4349 6152-6154 CD denotes 31
T4350 6154-6155 -RRB- denotes ]
T4351 6155-6156 . denotes .
T4352 6156-6300 sentence denotes These polymeric structures mediate the formation of a higher order chromatin structure and possibly stabilize the repressed state of Hox genes.
T4353 6157-6162 DT denotes These
T4354 6173-6183 NNS denotes structures
T4355 6163-6172 JJ denotes polymeric
T4356 6184-6191 VBP denotes mediate
T4357 6192-6195 DT denotes the
T4358 6196-6205 NN denotes formation
T4359 6206-6208 IN denotes of
T4360 6209-6210 DT denotes a
T4361 6234-6243 NN denotes structure
T4362 6211-6217 JJR denotes higher
T4363 6218-6223 NN denotes order
T4364 6224-6233 NN denotes chromatin
T4365 6244-6247 CC denotes and
T4366 6248-6256 RB denotes possibly
T4367 6257-6266 VBP denotes stabilize
T4368 6267-6270 DT denotes the
T4369 6281-6286 NN denotes state
T4370 6271-6280 VBN denotes repressed
T4371 6287-6289 IN denotes of
T4372 6290-6293 NN denotes Hox
T4373 6294-6299 NNS denotes genes
T4374 6299-6300 . denotes .
T4375 6300-6396 sentence denotes In this study, we identified a novel gene, mr-s, which encodes a SAM domain-containing protein.
T4376 6301-6303 IN denotes In
T4377 6319-6329 VBD denotes identified
T4378 6304-6308 DT denotes this
T4379 6309-6314 NN denotes study
T4380 6314-6316 , denotes ,
T4381 6316-6318 PRP denotes we
T4382 6330-6331 DT denotes a
T4383 6338-6342 NN denotes gene
T4384 6332-6337 JJ denotes novel
T4385 6342-6344 , denotes ,
T4386 6344-6346 NN denotes mr
T4387 6347-6348 NN denotes s
T4388 6346-6347 HYPH denotes -
T4389 6348-6350 , denotes ,
T4390 6350-6355 WDT denotes which
T4391 6356-6363 VBZ denotes encodes
T4392 6364-6365 DT denotes a
T4393 6388-6395 NN denotes protein
T4394 6366-6369 NN denotes SAM
T4395 6370-6376 NN denotes domain
T4396 6377-6387 VBG denotes containing
T4397 6376-6377 HYPH denotes -
T4398 6395-6396 . denotes .
T4399 6396-6485 sentence denotes The SAM domain of mr-s is most closely related to that of ph mouse ortholog, MPH1/Rae28.
T4400 6397-6400 DT denotes The
T4401 6405-6411 NN denotes domain
T4402 6401-6404 NN denotes SAM
T4403 6436-6443 VBN denotes related
T4404 6412-6414 IN denotes of
T4405 6415-6417 NN denotes mr
T4406 6418-6419 NN denotes s
T4407 6417-6418 HYPH denotes -
T4408 6420-6422 VBZ denotes is
T4409 6423-6427 RBS denotes most
T4410 6428-6435 RB denotes closely
T4411 6444-6446 IN denotes to
T4412 6447-6451 DT denotes that
T4413 6452-6454 IN denotes of
T4414 6455-6457 NN denotes ph
T4415 6464-6472 NN denotes ortholog
T4416 6458-6463 NN denotes mouse
T4417 6472-6474 , denotes ,
T4418 6474-6478 NN denotes MPH1
T4419 6479-6484 NN denotes Rae28
T4420 6478-6479 HYPH denotes /
T4421 6484-6485 . denotes .
T4422 6485-6611 sentence denotes mr-s is predominantly expressed in retinal photoreceptors when they undergo terminal differentiation, and adult pineal gland.
T4423 6486-6488 NN denotes mr
T4424 6489-6490 NN denotes s
T4425 6488-6489 HYPH denotes -
T4426 6508-6517 VBN denotes expressed
T4427 6491-6493 VBZ denotes is
T4428 6494-6507 RB denotes predominantly
T4429 6518-6520 IN denotes in
T4430 6521-6528 JJ denotes retinal
T4431 6529-6543 NNS denotes photoreceptors
T4432 6544-6548 WRB denotes when
T4433 6554-6561 VBP denotes undergo
T4434 6549-6553 PRP denotes they
T4435 6562-6570 JJ denotes terminal
T4436 6571-6586 NN denotes differentiation
T4437 6586-6588 , denotes ,
T4438 6588-6591 CC denotes and
T4439 6592-6597 JJ denotes adult
T4440 6605-6610 NN denotes gland
T4441 6598-6604 JJ denotes pineal
T4442 6610-6611 . denotes .
T4443 6611-6664 sentence denotes The expression of mr-s is directly regulated by Crx.
T4444 6612-6615 DT denotes The
T4445 6616-6626 NN denotes expression
T4446 6647-6656 VBN denotes regulated
T4447 6627-6629 IN denotes of
T4448 6630-6632 NN denotes mr
T4449 6633-6634 NN denotes s
T4450 6632-6633 HYPH denotes -
T4451 6635-6637 VBZ denotes is
T4452 6638-6646 RB denotes directly
T4453 6657-6659 IN denotes by
T4454 6660-6663 NN denotes Crx
T4455 6663-6664 . denotes .
T4456 6664-6772 sentence denotes Moreover, mr-s is localized in the nucleus and can self-associate through its SAM domain-containing region.
T4457 6665-6673 RB denotes Moreover
T4458 6683-6692 VBN denotes localized
T4459 6673-6675 , denotes ,
T4460 6675-6677 NN denotes mr
T4461 6678-6679 NN denotes s
T4462 6677-6678 HYPH denotes -
T4463 6680-6682 VBZ denotes is
T4464 6693-6695 IN denotes in
T4465 6696-6699 DT denotes the
T4466 6700-6707 NN denotes nucleus
T4467 6708-6711 CC denotes and
T4468 6712-6715 MD denotes can
T4469 6721-6730 VB denotes associate
T4470 6716-6720 NN denotes self
T4471 6720-6721 HYPH denotes -
T4472 6731-6738 IN denotes through
T4473 6739-6742 PRP$ denotes its
T4474 6765-6771 NN denotes region
T4475 6743-6746 NN denotes SAM
T4476 6747-6753 NN denotes domain
T4477 6754-6764 VBG denotes containing
T4478 6753-6754 HYPH denotes -
T4479 6771-6772 . denotes .
T4480 6772-6879 sentence denotes We also found that mr-s protein fused to GAL4 DNA-binding domain functions as a transcriptional repressor.
T4481 6773-6775 PRP denotes We
T4482 6781-6786 VBD denotes found
T4483 6776-6780 RB denotes also
T4484 6787-6791 IN denotes that
T4485 6838-6847 VBZ denotes functions
T4486 6792-6794 NN denotes mr
T4487 6795-6796 NN denotes s
T4488 6794-6795 HYPH denotes -
T4489 6797-6804 NN denotes protein
T4490 6805-6810 VBN denotes fused
T4491 6811-6813 IN denotes to
T4492 6814-6818 NN denotes GAL4
T4493 6831-6837 NN denotes domain
T4494 6819-6822 NN denotes DNA
T4495 6823-6830 VBG denotes binding
T4496 6822-6823 HYPH denotes -
T4497 6848-6850 IN denotes as
T4498 6851-6852 DT denotes a
T4499 6869-6878 NN denotes repressor
T4500 6853-6868 JJ denotes transcriptional
T4501 6878-6879 . denotes .
T4502 6879-7011 sentence denotes These findings suggest that mr-s functions as a member of a transcriptional repressor complex in retinal photoreceptor development.
T4503 6880-6885 DT denotes These
T4504 6886-6894 NNS denotes findings
T4505 6895-6902 VBP denotes suggest
T4506 6903-6907 IN denotes that
T4507 6913-6922 VBZ denotes functions
T4508 6908-6910 NN denotes mr
T4509 6911-6912 NN denotes s
T4510 6910-6911 HYPH denotes -
T4511 6923-6925 IN denotes as
T4512 6926-6927 DT denotes a
T4513 6928-6934 NN denotes member
T4514 6935-6937 IN denotes of
T4515 6938-6939 DT denotes a
T4516 6966-6973 NN denotes complex
T4517 6940-6955 JJ denotes transcriptional
T4518 6956-6965 NN denotes repressor
T4519 6974-6976 IN denotes in
T4520 6977-6984 JJ denotes retinal
T4521 6999-7010 NN denotes development
T4522 6985-6998 NN denotes photoreceptor
T4523 7010-7011 . denotes .
T5546 7022-7029 NN denotes Cloning
T5547 7030-7032 IN denotes of
T5548 7033-7038 NN denotes mouse
T5549 7042-7043 NN denotes s
T5550 7039-7041 NN denotes mr
T5551 7041-7042 HYPH denotes -
T5552 7043-7351 sentence denotes In order to identify novel mouse genes preferentially expressed in the developing retina, we screened the National Institute for Biotechnology Information (NCBI) database, UniGene, using Digital Differential Display (DDD) and found EST fragments which are frequently present in mouse retinal cDNA libraries.
T5553 7044-7046 IN denotes In
T5554 7137-7145 VBD denotes screened
T5555 7047-7052 NN denotes order
T5556 7053-7055 TO denotes to
T5557 7056-7064 VB denotes identify
T5558 7065-7070 JJ denotes novel
T5559 7077-7082 NNS denotes genes
T5560 7071-7076 NN denotes mouse
T5561 7083-7097 RB denotes preferentially
T5562 7098-7107 VBN denotes expressed
T5563 7108-7110 IN denotes in
T5564 7111-7114 DT denotes the
T5565 7126-7132 NN denotes retina
T5566 7115-7125 VBG denotes developing
T5567 7132-7134 , denotes ,
T5568 7134-7136 PRP denotes we
T5569 7146-7149 DT denotes the
T5570 7206-7214 NN denotes database
T5571 7150-7158 NNP denotes National
T5572 7159-7168 NNP denotes Institute
T5573 7169-7172 IN denotes for
T5574 7173-7186 NNP denotes Biotechnology
T5575 7187-7198 NNP denotes Information
T5576 7199-7200 -LRB- denotes (
T5577 7200-7204 NNP denotes NCBI
T5578 7204-7205 -RRB- denotes )
T5579 7214-7216 , denotes ,
T5580 7216-7223 NNP denotes UniGene
T5581 7223-7225 , denotes ,
T5582 7225-7230 VBG denotes using
T5583 7231-7238 NNP denotes Digital
T5584 7252-7259 NNP denotes Display
T5585 7239-7251 NNP denotes Differential
T5586 7260-7261 -LRB- denotes (
T5587 7261-7264 NNP denotes DDD
T5588 7264-7265 -RRB- denotes )
T5589 7266-7269 CC denotes and
T5590 7270-7275 VBD denotes found
T5591 7276-7279 NN denotes EST
T5592 7280-7289 NNS denotes fragments
T5593 7290-7295 WDT denotes which
T5594 7296-7299 VBP denotes are
T5595 7300-7310 RB denotes frequently
T5596 7311-7318 JJ denotes present
T5597 7319-7321 IN denotes in
T5598 7322-7327 NN denotes mouse
T5599 7341-7350 NNS denotes libraries
T5600 7328-7335 JJ denotes retinal
T5601 7336-7340 NN denotes cDNA
T5602 7350-7351 . denotes .
T5603 7351-7473 sentence denotes We found that one clone in these cDNAs encodes a protein containing a SAM domain related to that of polyhomeotic protein.
T5604 7352-7354 PRP denotes We
T5605 7355-7360 VBD denotes found
T5606 7361-7365 IN denotes that
T5607 7391-7398 VBZ denotes encodes
T5608 7366-7369 CD denotes one
T5609 7370-7375 NN denotes clone
T5610 7376-7378 IN denotes in
T5611 7379-7384 DT denotes these
T5612 7385-7390 NNS denotes cDNAs
T5613 7399-7400 DT denotes a
T5614 7401-7408 NN denotes protein
T5615 7409-7419 VBG denotes containing
T5616 7420-7421 DT denotes a
T5617 7426-7432 NN denotes domain
T5618 7422-7425 NN denotes SAM
T5619 7433-7440 VBN denotes related
T5620 7441-7443 IN denotes to
T5621 7444-7448 DT denotes that
T5622 7449-7451 IN denotes of
T5623 7452-7464 JJ denotes polyhomeotic
T5624 7465-7472 NN denotes protein
T5625 7472-7473 . denotes .
T5626 7473-7612 sentence denotes A PCR fragment corresponding to this mouse clone was used to screen a mouse P0-P3 retinal cDNA library to obtain a full-length cDNA clone.
T5627 7474-7475 DT denotes A
T5628 7480-7488 NN denotes fragment
T5629 7476-7479 NN denotes PCR
T5630 7527-7531 VBN denotes used
T5631 7489-7502 VBG denotes corresponding
T5632 7503-7505 IN denotes to
T5633 7506-7510 DT denotes this
T5634 7517-7522 NN denotes clone
T5635 7511-7516 NN denotes mouse
T5636 7523-7526 VBD denotes was
T5637 7532-7534 TO denotes to
T5638 7535-7541 VB denotes screen
T5639 7542-7543 DT denotes a
T5640 7569-7576 NN denotes library
T5641 7544-7549 NN denotes mouse
T5642 7550-7552 NN denotes P0
T5643 7553-7555 NN denotes P3
T5644 7552-7553 SYM denotes -
T5645 7556-7563 JJ denotes retinal
T5646 7564-7568 NN denotes cDNA
T5647 7577-7579 TO denotes to
T5648 7580-7586 VB denotes obtain
T5649 7587-7588 DT denotes a
T5650 7606-7611 NN denotes clone
T5651 7589-7593 JJ denotes full
T5652 7594-7600 NN denotes length
T5653 7593-7594 HYPH denotes -
T5654 7601-7605 NN denotes cDNA
T5655 7611-7612 . denotes .
T5656 7612-7711 sentence denotes Sequence analysis showed that this cDNA was a novel gene encoding a SAM domain-containing protein.
T5657 7613-7621 NN denotes Sequence
T5658 7622-7630 NN denotes analysis
T5659 7631-7637 VBD denotes showed
T5660 7638-7642 IN denotes that
T5661 7653-7656 VBD denotes was
T5662 7643-7647 DT denotes this
T5663 7648-7652 NN denotes cDNA
T5664 7657-7658 DT denotes a
T5665 7665-7669 NN denotes gene
T5666 7659-7664 JJ denotes novel
T5667 7670-7678 VBG denotes encoding
T5668 7679-7680 DT denotes a
T5669 7703-7710 NN denotes protein
T5670 7681-7684 NN denotes SAM
T5671 7685-7691 NN denotes domain
T5672 7692-7702 VBG denotes containing
T5673 7691-7692 HYPH denotes -
T5674 7710-7711 . denotes .
T5675 7711-7783 sentence denotes We referred to this protein as mr-s (major retinal SAM domain protein).
T5676 7712-7714 PRP denotes We
T5677 7715-7723 VBD denotes referred
T5678 7724-7726 IN denotes to
T5679 7727-7731 DT denotes this
T5680 7732-7739 NN denotes protein
T5681 7740-7742 IN denotes as
T5682 7743-7745 NN denotes mr
T5683 7746-7747 NN denotes s
T5684 7745-7746 HYPH denotes -
T5685 7748-7749 -LRB- denotes (
T5686 7749-7754 JJ denotes major
T5687 7774-7781 NN denotes protein
T5688 7755-7762 JJ denotes retinal
T5689 7763-7766 NN denotes SAM
T5690 7767-7773 NN denotes domain
T5691 7781-7782 -RRB- denotes )
T5692 7782-7783 . denotes .
T5693 7783-7896 sentence denotes As shown in Fig. 1A, a translation initiation codon is present in the same open reading frame as the SAM domain.
T5694 7784-7786 IN denotes As
T5695 7787-7792 VBN denotes shown
T5696 7836-7838 VBZ denotes is
T5697 7793-7795 IN denotes in
T5698 7796-7800 NN denotes Fig.
T5699 7801-7803 NN denotes 1A
T5700 7803-7805 , denotes ,
T5701 7805-7806 DT denotes a
T5702 7830-7835 NN denotes codon
T5703 7807-7818 NN denotes translation
T5704 7819-7829 NN denotes initiation
T5705 7839-7846 JJ denotes present
T5706 7847-7849 IN denotes in
T5707 7850-7853 DT denotes the
T5708 7872-7877 NN denotes frame
T5709 7854-7858 JJ denotes same
T5710 7859-7863 JJ denotes open
T5711 7864-7871 NN denotes reading
T5712 7878-7880 IN denotes as
T5713 7881-7884 DT denotes the
T5714 7889-7895 NN denotes domain
T5715 7885-7888 NN denotes SAM
T5716 7895-7896 . denotes .
T5717 7896-8053 sentence denotes This initiation site shows similarity to the consensus sequence proposed by Kozak [32] including the presence of the highly conserved purine at position -3.
T5718 7897-7901 DT denotes This
T5719 7913-7917 NN denotes site
T5720 7902-7912 NN denotes initiation
T5721 7918-7923 VBZ denotes shows
T5722 7924-7934 NN denotes similarity
T5723 7935-7937 IN denotes to
T5724 7938-7941 DT denotes the
T5725 7952-7960 NN denotes sequence
T5726 7942-7951 NN denotes consensus
T5727 7961-7969 VBN denotes proposed
T5728 7970-7972 IN denotes by
T5729 7973-7978 NN denotes Kozak
T5730 7979-7980 -LRB- denotes [
T5731 7980-7982 CD denotes 32
T5732 7982-7983 -RRB- denotes ]
T5733 7984-7993 VBG denotes including
T5734 7994-7997 DT denotes the
T5735 7998-8006 NN denotes presence
T5736 8007-8009 IN denotes of
T5737 8010-8013 DT denotes the
T5738 8031-8037 NN denotes purine
T5739 8014-8020 RB denotes highly
T5740 8021-8030 VBN denotes conserved
T5741 8038-8040 IN denotes at
T5742 8041-8049 NN denotes position
T5743 8050-8051 SYM denotes -
T5744 8051-8052 CD denotes 3
T5745 8052-8053 . denotes .
T5746 8053-8128 sentence denotes The stop codon of the predicted mr-s protein is also indicated in Fig. 1A.
T5747 8054-8057 DT denotes The
T5748 8063-8068 NN denotes codon
T5749 8058-8062 NN denotes stop
T5750 8107-8116 VBN denotes indicated
T5751 8069-8071 IN denotes of
T5752 8072-8075 DT denotes the
T5753 8091-8098 NN denotes protein
T5754 8076-8085 VBN denotes predicted
T5755 8086-8088 NN denotes mr
T5756 8089-8090 NN denotes s
T5757 8088-8089 HYPH denotes -
T5758 8099-8101 VBZ denotes is
T5759 8102-8106 RB denotes also
T5760 8117-8119 IN denotes in
T5761 8120-8124 NN denotes Fig.
T5762 8125-8127 NN denotes 1A
T5763 8127-8128 . denotes .
T5764 8128-8295 sentence denotes The amino acid sequence of the SAM domain of mr-s protein (Fig. 1A, boxed sequence) exhibits homology with SAM domains of EphB2, EphA4, MPH1, TEL and Smaug (Fig. 1B).
T5765 8129-8132 DT denotes The
T5766 8144-8152 NN denotes sequence
T5767 8133-8138 NN denotes amino
T5768 8139-8143 NN denotes acid
T5769 8213-8221 VBZ denotes exhibits
T5770 8153-8155 IN denotes of
T5771 8156-8159 DT denotes the
T5772 8164-8170 NN denotes domain
T5773 8160-8163 NN denotes SAM
T5774 8171-8173 IN denotes of
T5775 8174-8176 NN denotes mr
T5776 8177-8178 NN denotes s
T5777 8176-8177 HYPH denotes -
T5778 8179-8186 NN denotes protein
T5779 8187-8188 -LRB- denotes (
T5780 8193-8195 NN denotes 1A
T5781 8188-8192 NN denotes Fig.
T5782 8195-8197 , denotes ,
T5783 8197-8202 VBN denotes boxed
T5784 8203-8211 NN denotes sequence
T5785 8211-8212 -RRB- denotes )
T5786 8222-8230 NN denotes homology
T5787 8231-8235 IN denotes with
T5788 8236-8239 NN denotes SAM
T5789 8240-8247 NNS denotes domains
T5790 8248-8250 IN denotes of
T5791 8251-8256 NN denotes EphB2
T5792 8256-8258 , denotes ,
T5793 8258-8263 NN denotes EphA4
T5794 8263-8265 , denotes ,
T5795 8265-8269 NN denotes MPH1
T5796 8269-8271 , denotes ,
T5797 8271-8274 NN denotes TEL
T5798 8275-8278 CC denotes and
T5799 8279-8284 NN denotes Smaug
T5800 8285-8286 -LRB- denotes (
T5801 8291-8293 NN denotes 1B
T5802 8286-8290 NN denotes Fig.
T5803 8293-8294 -RRB- denotes )
T5804 8294-8295 . denotes .
T5805 8295-8424 sentence denotes By phylogenetic analysis, the SAM domain of mr-s is most closely related to that of Mph1/Rae28, a mouse homolog of ph (Fig. 1C).
T5806 8296-8298 IN denotes By
T5807 8361-8368 VBN denotes related
T5808 8299-8311 JJ denotes phylogenetic
T5809 8312-8320 NN denotes analysis
T5810 8320-8322 , denotes ,
T5811 8322-8325 DT denotes the
T5812 8330-8336 NN denotes domain
T5813 8326-8329 NN denotes SAM
T5814 8337-8339 IN denotes of
T5815 8340-8342 NN denotes mr
T5816 8343-8344 NN denotes s
T5817 8342-8343 HYPH denotes -
T5818 8345-8347 VBZ denotes is
T5819 8348-8352 RBS denotes most
T5820 8353-8360 RB denotes closely
T5821 8369-8371 IN denotes to
T5822 8372-8376 DT denotes that
T5823 8377-8379 IN denotes of
T5824 8380-8384 NN denotes Mph1
T5825 8385-8390 NN denotes Rae28
T5826 8384-8385 HYPH denotes /
T5827 8390-8392 , denotes ,
T5828 8392-8393 DT denotes a
T5829 8400-8407 NN denotes homolog
T5830 8394-8399 NN denotes mouse
T5831 8408-8410 IN denotes of
T5832 8411-8413 NN denotes ph
T5833 8414-8415 -LRB- denotes (
T5834 8420-8422 NN denotes 1C
T5835 8415-8419 NN denotes Fig.
T5836 8422-8423 -RRB- denotes )
T5837 8423-8424 . denotes .
T5838 8424-8578 sentence denotes Mouse mr-s protein is conserved in rat, human, chick and zebrafish, which display 91%, 70%, 36% and 26% identity with mouse mr-s, respectively (Fig. 1D).
T5839 8425-8430 NN denotes Mouse
T5840 8436-8443 NN denotes protein
T5841 8431-8433 NN denotes mr
T5842 8434-8435 NN denotes s
T5843 8433-8434 HYPH denotes -
T5844 8447-8456 VBN denotes conserved
T5845 8444-8446 VBZ denotes is
T5846 8457-8459 IN denotes in
T5847 8460-8463 NN denotes rat
T5848 8463-8465 , denotes ,
T5849 8465-8470 JJ denotes human
T5850 8470-8472 , denotes ,
T5851 8472-8477 NN denotes chick
T5852 8478-8481 CC denotes and
T5853 8482-8491 NN denotes zebrafish
T5854 8491-8493 , denotes ,
T5855 8493-8498 WDT denotes which
T5856 8499-8506 VBP denotes display
T5857 8507-8509 CD denotes 91
T5858 8509-8510 NN denotes %
T5859 8529-8537 NN denotes identity
T5860 8510-8512 , denotes ,
T5861 8512-8514 CD denotes 70
T5862 8514-8515 NN denotes %
T5863 8515-8517 , denotes ,
T5864 8517-8519 CD denotes 36
T5865 8519-8520 NN denotes %
T5866 8521-8524 CC denotes and
T5867 8525-8527 CD denotes 26
T5868 8527-8528 NN denotes %
T5869 8538-8542 IN denotes with
T5870 8543-8548 NN denotes mouse
T5871 8552-8553 NN denotes s
T5872 8549-8551 NN denotes mr
T5873 8551-8552 HYPH denotes -
T5874 8553-8555 , denotes ,
T5875 8555-8567 RB denotes respectively
T5876 8568-8569 -LRB- denotes (
T5877 8574-8576 NN denotes 1D
T5878 8569-8573 NN denotes Fig.
T5879 8576-8577 -RRB- denotes )
T5880 8577-8578 . denotes .
T5881 8578-8749 sentence denotes The SAM domains of rat, human, chick and zebrafish mr-s protein are highly conserved and display 96%, 90%, 76% and 72% identity with the SAM domain of mouse mr-s protein.
T5882 8579-8582 DT denotes The
T5883 8587-8594 NNS denotes domains
T5884 8583-8586 NN denotes SAM
T5885 8654-8663 VBN denotes conserved
T5886 8595-8597 IN denotes of
T5887 8598-8601 NN denotes rat
T5888 8635-8642 NN denotes protein
T5889 8601-8603 , denotes ,
T5890 8603-8608 JJ denotes human
T5891 8608-8610 , denotes ,
T5892 8610-8615 NN denotes chick
T5893 8616-8619 CC denotes and
T5894 8620-8629 NN denotes zebrafish
T5895 8630-8632 NN denotes mr
T5896 8633-8634 NN denotes s
T5897 8632-8633 HYPH denotes -
T5898 8643-8646 VBP denotes are
T5899 8647-8653 RB denotes highly
T5900 8664-8667 CC denotes and
T5901 8668-8675 VBP denotes display
T5902 8676-8678 CD denotes 96
T5903 8678-8679 NN denotes %
T5904 8698-8706 NN denotes identity
T5905 8679-8681 , denotes ,
T5906 8681-8683 CD denotes 90
T5907 8683-8684 NN denotes %
T5908 8684-8686 , denotes ,
T5909 8686-8688 CD denotes 76
T5910 8688-8689 NN denotes %
T5911 8690-8693 CC denotes and
T5912 8694-8696 CD denotes 72
T5913 8696-8697 NN denotes %
T5914 8707-8711 IN denotes with
T5915 8712-8715 DT denotes the
T5916 8720-8726 NN denotes domain
T5917 8716-8719 NN denotes SAM
T5918 8727-8729 IN denotes of
T5919 8730-8735 NN denotes mouse
T5920 8741-8748 NN denotes protein
T5921 8736-8738 NN denotes mr
T5922 8739-8740 NN denotes s
T5923 8738-8739 HYPH denotes -
T5924 8748-8749 . denotes .
T5925 8749-8897 sentence denotes The chromosomal localizations of mouse and human mr-s genes were determined by searching the mouse and human genome databases (NCBI), respectively.
T5926 8750-8753 DT denotes The
T5927 8766-8779 NNS denotes localizations
T5928 8754-8765 JJ denotes chromosomal
T5929 8815-8825 VBN denotes determined
T5930 8780-8782 IN denotes of
T5931 8783-8788 NN denotes mouse
T5932 8804-8809 NNS denotes genes
T5933 8789-8792 CC denotes and
T5934 8793-8798 JJ denotes human
T5935 8799-8801 NN denotes mr
T5936 8802-8803 NN denotes s
T5937 8801-8802 HYPH denotes -
T5938 8810-8814 VBD denotes were
T5939 8826-8828 IN denotes by
T5940 8829-8838 VBG denotes searching
T5941 8839-8842 DT denotes the
T5942 8866-8875 NNS denotes databases
T5943 8843-8848 NN denotes mouse
T5944 8849-8852 CC denotes and
T5945 8853-8858 JJ denotes human
T5946 8859-8865 NN denotes genome
T5947 8876-8877 -LRB- denotes (
T5948 8877-8881 NN denotes NCBI
T5949 8881-8882 -RRB- denotes )
T5950 8882-8884 , denotes ,
T5951 8884-8896 RB denotes respectively
T5952 8896-8897 . denotes .
T5953 8897-8985 sentence denotes Mouse mr-s is mapped to chromosome 4E2, and human MR-S is mapped to chromosome 1p36.33.
T5954 8898-8903 NN denotes Mouse
T5955 8907-8908 NN denotes s
T5956 8904-8906 NN denotes mr
T5957 8906-8907 HYPH denotes -
T5958 8912-8918 VBN denotes mapped
T5959 8909-8911 VBZ denotes is
T5960 8919-8921 IN denotes to
T5961 8922-8932 NN denotes chromosome
T5962 8933-8936 NN denotes 4E2
T5963 8936-8938 , denotes ,
T5964 8938-8941 CC denotes and
T5965 8942-8947 JJ denotes human
T5966 8951-8952 NN denotes S
T5967 8948-8950 NN denotes MR
T5968 8950-8951 HYPH denotes -
T5969 8956-8962 VBN denotes mapped
T5970 8953-8955 VBZ denotes is
T5971 8963-8965 IN denotes to
T5972 8966-8976 NN denotes chromosome
T5973 8977-8984 NN denotes 1p36.33
T5974 8984-8985 . denotes .
T5975 8985-9048 sentence denotes LCA is the most common cause of inherited childhood blindness.
T5976 8986-8989 NN denotes LCA
T5977 8990-8992 VBZ denotes is
T5978 8993-8996 DT denotes the
T5979 9009-9014 NN denotes cause
T5980 8997-9001 RBS denotes most
T5981 9002-9008 JJ denotes common
T5982 9015-9017 IN denotes of
T5983 9018-9027 VBN denotes inherited
T5984 9038-9047 NN denotes blindness
T5985 9028-9037 NN denotes childhood
T5986 9047-9048 . denotes .
T5987 9048-9149 sentence denotes Human MR-S maps in the vicinity region of the LCA9, recently identified as a new locus for LCA [33].
T5988 9049-9054 JJ denotes Human
T5989 9058-9059 NN denotes S
T5990 9055-9057 NN denotes MR
T5991 9057-9058 HYPH denotes -
T5992 9060-9064 VBZ denotes maps
T5993 9065-9067 IN denotes in
T5994 9068-9071 DT denotes the
T5995 9081-9087 NN denotes region
T5996 9072-9080 NN denotes vicinity
T5997 9088-9090 IN denotes of
T5998 9091-9094 DT denotes the
T5999 9095-9099 NN denotes LCA9
T6000 9099-9101 , denotes ,
T6001 9101-9109 RB denotes recently
T6002 9110-9120 VBN denotes identified
T6003 9121-9123 IN denotes as
T6004 9124-9125 DT denotes a
T6005 9130-9135 NN denotes locus
T6006 9126-9129 JJ denotes new
T6007 9136-9139 IN denotes for
T6008 9140-9143 NN denotes LCA
T6009 9144-9145 -LRB- denotes [
T6010 9145-9147 CD denotes 33
T6011 9147-9148 -RRB- denotes ]
T6012 9148-9149 . denotes .
T7365 10257-10267 NN denotes Expression
T7366 10268-10270 IN denotes of
T7367 10271-10273 NN denotes mr
T7368 10274-10275 NN denotes s
T7369 10273-10274 HYPH denotes -
T7370 10276-10278 IN denotes in
T7371 10279-10282 DT denotes the
T7372 10294-10300 NN denotes retina
T7373 10283-10293 VBG denotes developing
T7374 10301-10304 CC denotes and
T7375 10305-10308 DT denotes the
T7376 10316-10321 NN denotes gland
T7377 10309-10315 JJ denotes pineal
T7378 10321-10424 sentence denotes To investigate mr-s expression, we first performed whole mount in situ hybridization in mouse embryos.
T7379 10322-10324 TO denotes To
T7380 10325-10336 VB denotes investigate
T7381 10363-10372 VBD denotes performed
T7382 10337-10339 NN denotes mr
T7383 10340-10341 NN denotes s
T7384 10339-10340 HYPH denotes -
T7385 10342-10352 NN denotes expression
T7386 10352-10354 , denotes ,
T7387 10354-10356 PRP denotes we
T7388 10357-10362 RB denotes first
T7389 10373-10378 JJ denotes whole
T7390 10379-10384 NN denotes mount
T7391 10393-10406 NN denotes hybridization
T7392 10385-10387 FW denotes in
T7393 10388-10392 FW denotes situ
T7394 10407-10409 IN denotes in
T7395 10410-10415 NN denotes mouse
T7396 10416-10423 NNS denotes embryos
T7397 10423-10424 . denotes .
T7398 10424-10523 sentence denotes No hybridization signal was detected at E9.5, E10.5 and E11.5 with an mr-s probe (data not shown).
T7399 10425-10427 DT denotes No
T7400 10442-10448 NN denotes signal
T7401 10428-10441 NN denotes hybridization
T7402 10453-10461 VBN denotes detected
T7403 10449-10452 VBD denotes was
T7404 10462-10464 IN denotes at
T7405 10465-10469 NN denotes E9.5
T7406 10469-10471 , denotes ,
T7407 10471-10476 NN denotes E10.5
T7408 10477-10480 CC denotes and
T7409 10481-10486 NN denotes E11.5
T7410 10487-10491 IN denotes with
T7411 10492-10494 DT denotes an
T7412 10500-10505 NN denotes probe
T7413 10495-10497 NN denotes mr
T7414 10498-10499 NN denotes s
T7415 10497-10498 HYPH denotes -
T7416 10506-10507 -LRB- denotes (
T7417 10516-10521 VBN denotes shown
T7418 10507-10511 NNS denotes data
T7419 10512-10515 RB denotes not
T7420 10521-10522 -RRB- denotes )
T7421 10522-10523 . denotes .
T7422 10523-10647 sentence denotes We then investigated the expression of the mr-s gene in the developing retina by section in situ hybridization (Fig. 1A–G).
T7423 10524-10526 PRP denotes We
T7424 10532-10544 VBD denotes investigated
T7425 10527-10531 RB denotes then
T7426 10545-10548 DT denotes the
T7427 10549-10559 NN denotes expression
T7428 10560-10562 IN denotes of
T7429 10563-10566 DT denotes the
T7430 10572-10576 NN denotes gene
T7431 10567-10569 NN denotes mr
T7432 10570-10571 NN denotes s
T7433 10569-10570 HYPH denotes -
T7434 10577-10579 IN denotes in
T7435 10580-10583 DT denotes the
T7436 10595-10601 NN denotes retina
T7437 10584-10594 VBG denotes developing
T7438 10602-10604 IN denotes by
T7439 10605-10612 NN denotes section
T7440 10621-10634 NN denotes hybridization
T7441 10613-10615 FW denotes in
T7442 10616-10620 FW denotes situ
T7443 10635-10636 -LRB- denotes (
T7444 10641-10643 NN denotes 1A
T7445 10636-10640 NN denotes Fig.
T7446 10643-10644 SYM denotes –
T7447 10644-10645 NN denotes G
T7448 10645-10646 -RRB- denotes )
T7449 10646-10647 . denotes .
T7450 10647-10725 sentence denotes No significant signal was detected in the developing retina at E13 (Fig. 2A).
T7451 10648-10650 DT denotes No
T7452 10663-10669 NN denotes signal
T7453 10651-10662 JJ denotes significant
T7454 10674-10682 VBN denotes detected
T7455 10670-10673 VBD denotes was
T7456 10683-10685 IN denotes in
T7457 10686-10689 DT denotes the
T7458 10701-10707 NN denotes retina
T7459 10690-10700 VBG denotes developing
T7460 10708-10710 IN denotes at
T7461 10711-10714 NN denotes E13
T7462 10715-10716 -LRB- denotes (
T7463 10721-10723 NN denotes 2A
T7464 10716-10720 NN denotes Fig.
T7465 10723-10724 -RRB- denotes )
T7466 10724-10725 . denotes .
T7467 10725-10874 sentence denotes A weak signal was initially detected in the outer aspect of the neuroblastic layer (NBL), a presumptive photoreceptor layer at E18 (Fig. 2B, arrow).
T7468 10726-10727 DT denotes A
T7469 10733-10739 NN denotes signal
T7470 10728-10732 JJ denotes weak
T7471 10754-10762 VBN denotes detected
T7472 10740-10743 VBD denotes was
T7473 10744-10753 RB denotes initially
T7474 10763-10765 IN denotes in
T7475 10766-10769 DT denotes the
T7476 10776-10782 NN denotes aspect
T7477 10770-10775 JJ denotes outer
T7478 10783-10785 IN denotes of
T7479 10786-10789 DT denotes the
T7480 10803-10808 NN denotes layer
T7481 10790-10802 JJ denotes neuroblastic
T7482 10809-10810 -LRB- denotes (
T7483 10810-10813 NN denotes NBL
T7484 10813-10814 -RRB- denotes )
T7485 10814-10816 , denotes ,
T7486 10816-10817 DT denotes a
T7487 10844-10849 NN denotes layer
T7488 10818-10829 JJ denotes presumptive
T7489 10830-10843 NN denotes photoreceptor
T7490 10850-10852 IN denotes at
T7491 10853-10856 NN denotes E18
T7492 10857-10858 -LRB- denotes (
T7493 10863-10865 NN denotes 2B
T7494 10858-10862 NN denotes Fig.
T7495 10865-10867 , denotes ,
T7496 10867-10872 NN denotes arrow
T7497 10872-10873 -RRB- denotes )
T7498 10873-10874 . denotes .
T7499 10874-10970 sentence denotes At P3, an mr-s transcript was clearly detected in the developing photoreceptor layer (Fig. 2E).
T7500 10875-10877 IN denotes At
T7501 10913-10921 VBN denotes detected
T7502 10878-10880 NN denotes P3
T7503 10880-10882 , denotes ,
T7504 10882-10884 DT denotes an
T7505 10890-10900 NN denotes transcript
T7506 10885-10887 NN denotes mr
T7507 10888-10889 NN denotes s
T7508 10887-10888 HYPH denotes -
T7509 10901-10904 VBD denotes was
T7510 10905-10912 RB denotes clearly
T7511 10922-10924 IN denotes in
T7512 10925-10928 DT denotes the
T7513 10954-10959 NN denotes layer
T7514 10929-10939 VBG denotes developing
T7515 10940-10953 NN denotes photoreceptor
T7516 10960-10961 -LRB- denotes (
T7517 10966-10968 NN denotes 2E
T7518 10961-10965 NN denotes Fig.
T7519 10968-10969 -RRB- denotes )
T7520 10969-10970 . denotes .
T7521 10970-11043 sentence denotes At P6, mr-s showed peak expression in the photoreceptor layer (Fig. 2F).
T7522 10971-10973 IN denotes At
T7523 10983-10989 VBD denotes showed
T7524 10974-10976 NN denotes P6
T7525 10976-10978 , denotes ,
T7526 10978-10980 NN denotes mr
T7527 10981-10982 NN denotes s
T7528 10980-10981 HYPH denotes -
T7529 10990-10994 NN denotes peak
T7530 10995-11005 NN denotes expression
T7531 11006-11008 IN denotes in
T7532 11009-11012 DT denotes the
T7533 11027-11032 NN denotes layer
T7534 11013-11026 NN denotes photoreceptor
T7535 11033-11034 -LRB- denotes (
T7536 11039-11041 NN denotes 2F
T7537 11034-11038 NN denotes Fig.
T7538 11041-11042 -RRB- denotes )
T7539 11042-11043 . denotes .
T7540 11043-11178 sentence denotes This pattern correlates with the rapid increase in cells expressing rhodopsin and other phototransduction genes between P6-P8 [34-37].
T7541 11044-11048 DT denotes This
T7542 11049-11056 NN denotes pattern
T7543 11057-11067 VBZ denotes correlates
T7544 11068-11072 IN denotes with
T7545 11073-11076 DT denotes the
T7546 11083-11091 NN denotes increase
T7547 11077-11082 JJ denotes rapid
T7548 11092-11094 IN denotes in
T7549 11095-11100 NNS denotes cells
T7550 11101-11111 VBG denotes expressing
T7551 11112-11121 NN denotes rhodopsin
T7552 11122-11125 CC denotes and
T7553 11126-11131 JJ denotes other
T7554 11150-11155 NNS denotes genes
T7555 11132-11149 NN denotes phototransduction
T7590 11291-11294 NN denotes ONL
T7591 11294-11295 -RRB- denotes )
T7592 11296-11297 -LRB- denotes (
T7593 11306-11311 VBN denotes shown
T7594 11297-11301 NNS denotes data
T7595 11302-11305 RB denotes not
T7596 11311-11312 -RRB- denotes )
T7597 11312-11313 . denotes .
T7598 11313-11412 sentence denotes Faint expression of mr-s mRNA was observed in mature photoreceptors in the adult retina (Fig. 2G).
T7599 11314-11319 NN denotes Faint
T7600 11320-11330 NN denotes expression
T7601 11348-11356 VBN denotes observed
T7602 11331-11333 IN denotes of
T7603 11334-11336 NN denotes mr
T7604 11337-11338 NN denotes s
T7605 11336-11337 HYPH denotes -
T7606 11339-11343 NN denotes mRNA
T7607 11344-11347 VBD denotes was
T7608 11357-11359 IN denotes in
T7609 11360-11366 JJ denotes mature
T7610 11367-11381 NNS denotes photoreceptors
T7611 11382-11384 IN denotes in
T7612 11385-11388 DT denotes the
T7613 11395-11401 NN denotes retina
T7614 11389-11394 JJ denotes adult
T7615 11402-11403 -LRB- denotes (
T7616 11408-11410 NN denotes 2G
T7617 11403-11407 NN denotes Fig.
T7618 11410-11411 -RRB- denotes )
T7619 11411-11412 . denotes .
T7620 11412-11413 sentence denotes
T7621 12399-12406 sentence denotes To dete
T7622 12399-12400 DT denotes T
T7623 12400-12401 NN denotes o
T7624 12402-12406 VBN denotes dete
T7662 12459-12469 NN denotes expression
T7663 12470-12472 IN denotes of
T7664 12473-12476 DT denotes the
T7665 12482-12486 NN denotes gene
T7666 12477-12479 NN denotes mr
T7667 12480-12481 NN denotes s
T7668 12479-12480 HYPH denotes -
T7669 12487-12489 IN denotes in
T7670 12490-12497 JJ denotes various
T7671 12504-12511 NNS denotes tissues
T7672 12498-12503 JJ denotes adult
T7673 12512-12515 VBD denotes was
T7674 12525-12527 IN denotes by
T7675 12528-12536 NNP denotes Northern
T7676 12537-12550 NN denotes hybridization
T7677 12551-12552 -LRB- denotes (
T7678 12557-12559 NN denotes 2H
T7679 12552-12556 NN denotes Fig.
T7680 12559-12560 -RRB- denotes )
T7681 12560-12561 . denotes .
T7682 12561-12600 sentence denotes As a control, P7 retinal RNA was used.
T7683 12562-12564 IN denotes As
T7684 12595-12599 VBN denotes used
T7685 12565-12566 DT denotes a
T7686 12567-12574 NN denotes control
T7687 12574-12576 , denotes ,
T7688 12576-12578 NN denotes P7
T7689 12587-12590 NN denotes RNA
T7690 12579-12586 NN denotes retinal
T7691 12591-12594 VBD denotes was
T7692 12599-12600 . denotes .
T7693 12600-12690 sentence denotes Four bands corresponding to 7.2-kb, 4.0-kb, 2.5-kb and 2.2-kb were detected in P7 retina.
T7694 12601-12605 CD denotes Four
T7695 12606-12611 NNS denotes bands
T7696 12668-12676 VBN denotes detected
T7697 12612-12625 VBG denotes corresponding
T7698 12626-12628 IN denotes to
T7699 12629-12632 CD denotes 7.2
T7700 12633-12635 NN denotes kb
T7701 12632-12633 HYPH denotes -
T7702 12635-12637 , denotes ,
T7703 12637-12640 CD denotes 4.0
T7704 12641-12643 NN denotes kb
T7705 12640-12641 HYPH denotes -
T7706 12643-12645 , denotes ,
T7707 12645-12648 CD denotes 2.5
T7708 12649-12651 NN denotes kb
T7709 12648-12649 HYPH denotes -
T7710 12652-12655 CC denotes and
T7711 12656-12659 CD denotes 2.2
T7712 12660-12662 NN denotes kb
T7713 12659-12660 HYPH denotes -
T7714 12663-12667 VBD denotes were
T7715 12677-12679 IN denotes in
T7716 12680-12682 NN denotes P7
T7717 12683-12689 NN denotes retina
T7718 12689-12690 . denotes .
T7719 12690-12759 sentence denotes The 2.2-kb band corresponds to the cDNA characterized in this study.
T7720 12691-12694 DT denotes The
T7721 12702-12706 NN denotes band
T7722 12695-12698 CD denotes 2.2
T7723 12699-12701 NN denotes kb
T7724 12698-12699 HYPH denotes -
T7725 12707-12718 VBZ denotes corresponds
T7726 12719-12721 IN denotes to
T7727 12722-12725 DT denotes the
T7728 12726-12730 NN denotes cDNA
T7729 12731-12744 VBN denotes characterized
T7730 12745-12747 IN denotes in
T7731 12748-12752 DT denotes this
T7732 12753-12758 NN denotes study
T7733 12758-12759 . denotes .
T7734 12759-12852 sentence denotes The larger bands, possibly alternative spliced transcripts, have not yet been characterized.
T7735 12760-12763 DT denotes The
T7736 12771-12776 NNS denotes bands
T7737 12764-12770 JJR denotes larger
T7738 12838-12851 VBN denotes characterized
T7739 12776-12778 , denotes ,
T7740 12778-12786 RB denotes possibly
T7741 12807-12818 NNS denotes transcripts
T7742 12787-12798 JJ denotes alternative
T7743 12799-12806 VBN denotes spliced
T7744 12818-12820 , denotes ,
T7745 12820-12824 VBP denotes have
T7746 12825-12828 RB denotes not
T7747 12829-12832 RB denotes yet
T7748 12833-12837 VBN denotes been
T7749 12851-12852 . denotes .
T7750 12852-13026 sentence denotes The mr-s probe did not detect a band in the adult tissues examined, indicating that these tissues do not express mr-s at a level comparable to that of the developing retina.
T7751 12853-12856 DT denotes The
T7752 12862-12867 NN denotes probe
T7753 12857-12859 NN denotes mr
T7754 12860-12861 NN denotes s
T7755 12859-12860 HYPH denotes -
T7756 12876-12882 VB denotes detect
T7757 12868-12871 VBD denotes did
T7758 12872-12875 RB denotes not
T7759 12883-12884 DT denotes a
T7760 12885-12889 NN denotes band
T7761 12890-12892 IN denotes in
T7762 12893-12896 DT denotes the
T7763 12903-12910 NNS denotes tissues
T7764 12897-12902 JJ denotes adult
T7765 12911-12919 VBN denotes examined
T7766 12919-12921 , denotes ,
T7767 12921-12931 VBG denotes indicating
T7768 12932-12936 IN denotes that
T7769 12958-12965 VB denotes express
T7770 12937-12942 DT denotes these
T7771 12943-12950 NNS denotes tissues
T7772 12951-12953 VBP denotes do
T7773 12954-12957 RB denotes not
T7774 12966-12968 NN denotes mr
T7775 12969-12970 NN denotes s
T7776 12968-12969 HYPH denotes -
T7777 12971-12973 IN denotes at
T7778 12974-12975 DT denotes a
T7779 12976-12981 NN denotes level
T7780 12982-12992 JJ denotes comparable
T7781 12993-12995 IN denotes to
T7782 12996-13000 DT denotes that
T7783 13001-13003 IN denotes of
T7784 13004-13007 DT denotes the
T7785 13019-13025 NN denotes retina
T7786 13008-13018 VBG denotes developing
T7787 13025-13026 . denotes .
T7788 13026-13136 sentence denotes Previous reports revealed that many photoreceptor-specific genes are also expressed in the pineal gland [38].
T7789 13027-13035 JJ denotes Previous
T7790 13036-13043 NNS denotes reports
T7791 13044-13052 VBD denotes revealed
T7792 13053-13057 IN denotes that
T7793 13101-13110 VBN denotes expressed
T7794 13058-13062 JJ denotes many
T7795 13086-13091 NNS denotes genes
T7796 13063-13076 NN denotes photoreceptor
T7797 13077-13085 JJ denotes specific
T7798 13076-13077 HYPH denotes -
T7799 13092-13095 VBP denotes are
T7800 13096-13100 RB denotes also
T7801 13111-13113 IN denotes in
T7802 13114-13117 DT denotes the
T7803 13125-13130 NN denotes gland
T7804 13118-13124 JJ denotes pineal
T7805 13131-13132 -LRB- denotes [
T7806 13132-13134 CD denotes 38
T7807 13134-13135 -RRB- denotes ]
T7808 13135-13136 . denotes .
T7809 13136-13326 sentence denotes We examined the expression of mr-s transcripts in the whole embryo, whole body, retina, pineal gland, brain, liver and other organs at various stages by RT-PCR (Fig. 2I and data not shown).
T7810 13137-13139 PRP denotes We
T7811 13140-13148 VBD denotes examined
T7812 13149-13152 DT denotes the
T7813 13153-13163 NN denotes expression
T7814 13164-13166 IN denotes of
T7815 13167-13169 NN denotes mr
T7816 13170-13171 NN denotes s
T7817 13169-13170 HYPH denotes -
T7818 13172-13183 NNS denotes transcripts
T7819 13184-13186 IN denotes in
T7820 13187-13190 DT denotes the
T7821 13197-13203 NN denotes embryo
T7822 13191-13196 JJ denotes whole
T7823 13203-13205 , denotes ,
T7824 13205-13210 JJ denotes whole
T7825 13211-13215 NN denotes body
T7826 13215-13217 , denotes ,
T7827 13217-13223 NN denotes retina
T7828 13223-13225 , denotes ,
T7829 13225-13231 JJ denotes pineal
T7830 13232-13237 NN denotes gland
T7831 13237-13239 , denotes ,
T7832 13239-13244 NN denotes brain
T7833 13244-13246 , denotes ,
T7834 13246-13251 NN denotes liver
T7835 13252-13255 CC denotes and
T7836 13256-13261 JJ denotes other
T7837 13262-13268 NNS denotes organs
T7838 13269-13271 IN denotes at
T7839 13272-13279 JJ denotes various
T7840 13280-13286 NNS denotes stages
T7841 13287-13289 IN denotes by
T7842 13290-13292 NN denotes RT
T7843 13293-13296 NN denotes PCR
T7844 13292-13293 HYPH denotes -
T7845 13297-13298 -LRB- denotes (
T7846 13303-13305 NN denotes 2I
T7847 13298-13302 NN denotes Fig.
T7848 13306-13309 CC denotes and
T7849 13310-13314 NNS denotes data
T7850 13319-13324 VBN denotes shown
T7851 13315-13318 RB denotes not
T7852 13324-13325 -RRB- denotes )
T7853 13325-13326 . denotes .
T7854 13326-13447 sentence denotes We amplified PCR fragments of 292 bp and 452 bp with primer pairs for genes encoding mouse mr-s and G3PDH, respectively.
T7855 13327-13329 PRP denotes We
T7856 13330-13339 VBD denotes amplified
T7857 13340-13343 NN denotes PCR
T7858 13344-13353 NNS denotes fragments
T7859 13354-13356 IN denotes of
T7860 13357-13360 CD denotes 292
T7861 13361-13363 NN denotes bp
T7862 13364-13367 CC denotes and
T7864 13372-13374 NN denotes bp
T7865 13375-13379 IN denotes with
T7866 13380-13386 NN denotes primer
T7867 13387-13392 NNS denotes pairs
T7868 13393-13396 IN denotes for
T7869 13397-13402 NNS denotes genes
T7870 13403-13411 VBG denotes encoding
T7871 13412-13417 NN denotes mouse
T7872 13421-13422 NN denotes s
T7873 13418-13420 NN denotes mr
T7874 13420-13421 HYPH denotes -
T7875 13423-13426 CC denotes and
T7876 13427-13432 NN denotes G3PDH
T7877 13432-13434 , denotes ,
T7878 13434-13446 RB denotes respectively
T7879 13446-13447 . denotes .
T7880 13447-13536 sentence denotes In E13 whole embryo and P0 whole body (except for the eye), no mr-s signal was detected.
T7881 13448-13450 IN denotes In
T7882 13527-13535 VBN denotes detected
T7883 13451-13454 NN denotes E13
T7884 13461-13467 NN denotes embryo
T7885 13455-13460 JJ denotes whole
T7886 13468-13471 CC denotes and
T7887 13472-13474 NN denotes P0
T7888 13481-13485 NN denotes body
T7889 13475-13480 JJ denotes whole
T7890 13486-13487 -LRB- denotes (
T7891 13487-13493 IN denotes except
T7892 13494-13497 IN denotes for
T7893 13498-13501 DT denotes the
T7894 13502-13505 NN denotes eye
T7895 13505-13506 -RRB- denotes )
T7896 13506-13508 , denotes ,
T7897 13508-13510 DT denotes no
T7898 13516-13522 NN denotes signal
T7899 13511-13513 NN denotes mr
T7900 13514-13515 NN denotes s
T7901 13513-13514 HYPH denotes -
T7902 13523-13526 VBD denotes was
T7903 13535-13536 . denotes .
T7904 13536-13618 sentence denotes As expected, we observed that mr-s is expressed in the P7 and adult pineal gland.
T7905 13537-13539 IN denotes As
T7906 13540-13548 VBN denotes expected
T7907 13553-13561 VBD denotes observed
T7908 13548-13550 , denotes ,
T7909 13550-13552 PRP denotes we
T7910 13562-13566 IN denotes that
T7911 13575-13584 VBN denotes expressed
T7912 13567-13569 NN denotes mr
T7913 13570-13571 NN denotes s
T7914 13569-13570 HYPH denotes -
T7915 13572-13574 VBZ denotes is
T7916 13585-13587 IN denotes in
T7917 13588-13591 DT denotes the
T7918 13612-13617 NN denotes gland
T7919 13592-13594 NN denotes P7
T7920 13595-13598 CC denotes and
T7921 13599-13604 JJ denotes adult
T7922 13605-13611 JJ denotes pineal
T7923 13617-13618 . denotes .
T7924 13618-13758 sentence denotes In the P7 and adult brain, liver and several other organs, the RT-PCR amplified band of mr-s was not detected (Fig. 2I and data not shown).
T7925 13619-13621 IN denotes In
T7926 13720-13728 VBN denotes detected
T7927 13622-13625 DT denotes the
T7928 13639-13644 NN denotes brain
T7929 13626-13628 NN denotes P7
T7930 13629-13632 CC denotes and
T7931 13633-13638 JJ denotes adult
T7932 13644-13646 , denotes ,
T7933 13646-13651 NN denotes liver
T7934 13652-13655 CC denotes and
T7935 13656-13663 JJ denotes several
T7936 13670-13676 NNS denotes organs
T7937 13664-13669 JJ denotes other
T7938 13676-13678 , denotes ,
T7939 13678-13681 DT denotes the
T7940 13699-13703 NN denotes band
T7941 13682-13684 NN denotes RT
T7942 13685-13688 NN denotes PCR
T7943 13684-13685 HYPH denotes -
T7944 13689-13698 VBN denotes amplified
T7945 13704-13706 IN denotes of
T7946 13707-13709 NN denotes mr
T7947 13710-13711 NN denotes s
T7948 13709-13710 HYPH denotes -
T7949 13712-13715 VBD denotes was
T7950 13716-13719 RB denotes not
T7951 13729-13730 -LRB- denotes (
T7952 13735-13737 NN denotes 2I
T7953 13730-13734 NN denotes Fig.
T7954 13738-13741 CC denotes and
T7955 13742-13746 NNS denotes data
T7956 13751-13756 VBN denotes shown
T7957 13747-13750 RB denotes not
T7958 13756-13757 -RRB- denotes )
T7959 13757-13758 . denotes .
T7960 13758-13862 sentence denotes Our data showed that mr-s is predominantly expressed in developing photoreceptors and the pineal gland.
T7961 13759-13762 PRP$ denotes Our
T7962 13763-13767 NNS denotes data
T7963 13768-13774 VBD denotes showed
T7964 13775-13779 IN denotes that
T7965 13802-13811 VBN denotes expressed
T7966 13780-13782 NN denotes mr
T7967 13783-13784 NN denotes s
T7968 13782-13783 HYPH denotes -
T7969 13785-13787 VBZ denotes is
T7970 13788-13801 RB denotes predominantly
T7971 13812-13814 IN denotes in
T7972 13815-13825 VBG denotes developing
T7973 13826-13840 NNS denotes photoreceptors
T7974 13841-13844 CC denotes and
T7975 13845-13848 DT denotes the
T7976 13856-13861 NN denotes gland
T7977 13849-13855 JJ denotes pineal
T7978 13861-13862 . denotes .
T9715 13864-13874 NN denotes Regulation
T9716 13875-13877 IN denotes of
T9717 13878-13880 NN denotes mr
T9718 13881-13882 NN denotes s
T9719 13880-13881 HYPH denotes -
T9720 13883-13896 NN denotes transcription
T9721 13897-13899 IN denotes by
T9722 13900-13903 NN denotes Crx
T9723 13916-13923 NN denotes protein
T9724 13904-13915 NN denotes homeodomain
T9725 13923-14035 sentence denotes Transcription factors Otx2 and Crx are known to be key regulators of retinal photoreceptor development [3,8,9].
T9726 13924-13937 NN denotes Transcription
T9727 13938-13945 NNS denotes factors
T9728 13963-13968 VBN denotes known
T9729 13946-13950 NN denotes Otx2
T9730 13951-13954 CC denotes and
T9731 13955-13958 NN denotes Crx
T9732 13959-13962 VBP denotes are
T9733 13969-13971 TO denotes to
T9734 13972-13974 VB denotes be
T9735 13975-13978 JJ denotes key
T9736 13979-13989 NNS denotes regulators
T9737 13990-13992 IN denotes of
T9738 13993-14000 JJ denotes retinal
T9739 14015-14026 NN denotes development
T9740 14001-14014 NN denotes photoreceptor
T9741 14027-14028 -LRB- denotes [
T9742 14032-14033 CD denotes 9
T9743 14028-14029 CD denotes 3
T9744 14029-14030 , denotes ,
T9745 14030-14031 CD denotes 8
T9746 14031-14032 , denotes ,
T9747 14033-14034 -RRB- denotes ]
T9748 14034-14035 . denotes .
T9749 14035-14243 sentence denotes Although both Otx2 and Crx are expressed in developing photoreceptor cells in the embryonic retina, only Crx is highly expressed in the postnatal photoreceptors, suggesting that mr-s may be regulated by Crx.
T9750 14036-14044 IN denotes Although
T9751 14067-14076 VBN denotes expressed
T9752 14045-14049 CC denotes both
T9753 14050-14054 NN denotes Otx2
T9754 14055-14058 CC denotes and
T9755 14059-14062 NN denotes Crx
T9756 14063-14066 VBP denotes are
T9757 14155-14164 VBN denotes expressed
T9758 14077-14079 IN denotes in
T9759 14080-14090 VBG denotes developing
T9760 14105-14110 NNS denotes cells
T9761 14091-14104 NN denotes photoreceptor
T9762 14111-14113 IN denotes in
T9763 14114-14117 DT denotes the
T9764 14128-14134 NN denotes retina
T9765 14118-14127 JJ denotes embryonic
T9766 14134-14136 , denotes ,
T9767 14136-14140 RB denotes only
T9768 14141-14144 NN denotes Crx
T9769 14145-14147 VBZ denotes is
T9770 14148-14154 RB denotes highly
T9771 14165-14167 IN denotes in
T9772 14168-14171 DT denotes the
T9773 14182-14196 NNS denotes photoreceptors
T9774 14172-14181 JJ denotes postnatal
T9775 14196-14198 , denotes ,
T9776 14198-14208 VBG denotes suggesting
T9777 14209-14213 IN denotes that
T9778 14226-14235 VBN denotes regulated
T9779 14214-14216 NN denotes mr
T9780 14217-14218 NN denotes s
T9781 14216-14217 HYPH denotes -
T9782 14219-14222 MD denotes may
T9783 14223-14225 VB denotes be
T9784 14236-14238 IN denotes by
T9785 14239-14242 NN denotes Crx
T9786 14242-14243 . denotes .
T9787 14243-14390 sentence denotes To test whether Crx regulates mr-s transcription, we performed in situ hybridization of mr-s mRNA on wild-type and Crx KO P5 retinas (Fig. 3A, B).
T9788 14244-14246 TO denotes To
T9789 14247-14251 VB denotes test
T9790 14297-14306 VBD denotes performed
T9791 14252-14259 IN denotes whether
T9792 14264-14273 VBZ denotes regulates
T9793 14260-14263 NN denotes Crx
T9794 14274-14276 NN denotes mr
T9795 14277-14278 NN denotes s
T9796 14276-14277 HYPH denotes -
T9797 14279-14292 NN denotes transcription
T9798 14292-14294 , denotes ,
T9799 14294-14296 PRP denotes we
T9800 14307-14309 FW denotes in
T9801 14310-14314 FW denotes situ
T9802 14315-14328 NN denotes hybridization
T9803 14329-14331 IN denotes of
T9804 14332-14334 NN denotes mr
T9805 14335-14336 NN denotes s
T9806 14334-14335 HYPH denotes -
T9807 14337-14341 NN denotes mRNA
T9808 14342-14344 IN denotes on
T9809 14345-14349 JJ denotes wild
T9810 14350-14354 NN denotes type
T9811 14349-14350 HYPH denotes -
T9812 14369-14376 NNS denotes retinas
T9813 14355-14358 CC denotes and
T9814 14359-14362 NN denotes Crx
T9815 14363-14365 NN denotes KO
T9816 14366-14368 NN denotes P5
T9817 14377-14378 -LRB- denotes (
T9818 14387-14388 NN denotes B
T9819 14378-14382 NN denotes Fig.
T9820 14383-14385 NN denotes 3A
T9821 14385-14387 , denotes ,
T9822 14388-14389 -RRB- denotes )
T9823 14389-14390 . denotes .
T9824 14390-14468 sentence denotes In the Crx KO retina, the mr-s transcript was dramatically reduced (Fig. 3B).
T9825 14391-14393 IN denotes In
T9826 14450-14457 VBN denotes reduced
T9827 14394-14397 DT denotes the
T9828 14405-14411 NN denotes retina
T9829 14398-14401 NN denotes Crx
T9830 14402-14404 NN denotes KO
T9831 14411-14413 , denotes ,
T9832 14413-14416 DT denotes the
T9833 14422-14432 NN denotes transcript
T9834 14417-14419 NN denotes mr
T9835 14420-14421 NN denotes s
T9836 14419-14420 HYPH denotes -
T9837 14433-14436 VBD denotes was
T9838 14437-14449 RB denotes dramatically
T9839 14458-14459 -LRB- denotes (
T9840 14464-14466 NN denotes 3B
T9841 14459-14463 NN denotes Fig.
T9842 14466-14467 -RRB- denotes )
T9843 14467-14468 . denotes .
T9844 14468-14534 sentence denotes This indicates that Crx is essential for transactivation of mr-s.
T9845 14469-14473 DT denotes This
T9846 14474-14483 VBZ denotes indicates
T9847 14484-14488 IN denotes that
T9848 14493-14495 VBZ denotes is
T9849 14489-14492 NN denotes Crx
T9850 14496-14505 JJ denotes essential
T9851 14506-14509 IN denotes for
T9852 14510-14525 NN denotes transactivation
T9853 14526-14528 IN denotes of
T9854 14529-14531 NN denotes mr
T9855 14532-14533 NN denotes s
T9856 14531-14532 HYPH denotes -
T9857 14533-14534 . denotes .
T9858 14534-14751 sentence denotes Moreover, to test whether the transcription of mr-s is also regulated by Crx in the pineal gland, RT-PCR analysis was used to compare transcriptional levels of mr-s in P28 wild-type and Crx KO pineal gland (Fig. 3C).
T9859 14535-14543 RB denotes Moreover
T9860 14653-14657 VBN denotes used
T9861 14543-14545 , denotes ,
T9862 14545-14547 TO denotes to
T9863 14548-14552 VB denotes test
T9864 14553-14560 IN denotes whether
T9865 14595-14604 VBN denotes regulated
T9866 14561-14564 DT denotes the
T9867 14565-14578 NN denotes transcription
T9868 14579-14581 IN denotes of
T9869 14582-14584 NN denotes mr
T9870 14585-14586 NN denotes s
T9871 14584-14585 HYPH denotes -
T9872 14587-14589 VBZ denotes is
T9873 14590-14594 RB denotes also
T9874 14605-14607 IN denotes by
T9875 14608-14611 NN denotes Crx
T9876 14612-14614 IN denotes in
T9877 14615-14618 DT denotes the
T9878 14626-14631 NN denotes gland
T9879 14619-14625 JJ denotes pineal
T9880 14631-14633 , denotes ,
T9881 14633-14635 NN denotes RT
T9882 14636-14639 NN denotes PCR
T9883 14635-14636 HYPH denotes -
T9884 14640-14648 NN denotes analysis
T9885 14649-14652 VBD denotes was
T9886 14658-14660 TO denotes to
T9887 14661-14668 VB denotes compare
T9888 14669-14684 JJ denotes transcriptional
T9889 14685-14691 NNS denotes levels
T9890 14692-14694 IN denotes of
T9891 14695-14697 NN denotes mr
T9892 14698-14699 NN denotes s
T9893 14697-14698 HYPH denotes -
T9894 14700-14702 IN denotes in
T9895 14703-14706 NN denotes P28
T9896 14735-14740 NN denotes gland
T9897 14707-14711 JJ denotes wild
T9898 14712-14716 NN denotes type
T9899 14711-14712 HYPH denotes -
T9900 14717-14720 CC denotes and
T9901 14721-14724 NN denotes Crx
T9902 14725-14727 NN denotes KO
T9903 14728-14734 JJ denotes pineal
T9904 14741-14742 -LRB- denotes (
T9905 14747-14749 NN denotes 3C
T9906 14742-14746 NN denotes Fig.
T9907 14749-14750 -RRB- denotes )
T9908 14750-14751 . denotes .
T9909 14751-14856 sentence denotes The results revealed that mr-s transcription was significantly downregulated in the Crx KO pineal gland.
T9910 14752-14755 DT denotes The
T9911 14756-14763 NNS denotes results
T9912 14764-14772 VBD denotes revealed
T9913 14773-14777 IN denotes that
T9914 14815-14828 VBN denotes downregulated
T9962 16037-16038 NN denotes e
T9963 16038-16039 IN denotes r
T9964 16040-16041 NN denotes e
T9965 16042-16043 NNS denotes a
T9966 16041-16042 NN denotes x
T9967 16043-16048 sentence denotes mine
T9968 16043-16044 NN denotes m
T9969 16044-16046 NN denotes in
T9970 16046-16047 VBN denotes e
T10020 16160-16168 JJ denotes proximal
T10021 16169-16177 NN denotes promoter
T10022 16185-16187 IN denotes of
T10023 16188-16190 NN denotes mr
T10024 16191-16192 NN denotes s
T10025 16190-16191 HYPH denotes -
T10026 16193-16198 VBN denotes fused
T10027 16199-16201 IN denotes to
T10028 16202-16203 DT denotes a
T10029 16215-16219 NN denotes gene
T10030 16204-16214 NN denotes luciferase
T10031 16220-16222 IN denotes as
T10032 16223-16224 DT denotes a
T10033 16236-16244 NN denotes reporter
T10034 16225-16235 NN denotes luciferase
T10035 16245-16246 -LRB- denotes (
T10036 16251-16253 NN denotes 3D
T10037 16246-16250 NN denotes Fig.
T10038 16253-16255 , denotes ,
T10039 16255-16262 NN denotes Pro1.2k
T10040 16262-16263 -RRB- denotes )
T10041 16264-16267 CC denotes and
T10042 16268-16271 DT denotes the
T10043 16298-16305 NNS denotes vectors
T10044 16272-16275 NN denotes Crx
T10045 16283-16286 NN denotes Nrl
T10046 16275-16277 , denotes ,
T10047 16277-16281 NN denotes Otx2
T10048 16281-16283 , denotes ,
T10049 16287-16297 NN denotes expression
T10050 16305-16307 , denotes ,
T10051 16307-16319 RB denotes respectively
T10052 16319-16320 . denotes .
T10053 16320-16417 sentence denotes This 1.2-kb region of the mr-s upstream sequence contains three Crx binding consensus sequences.
T10054 16321-16325 DT denotes This
T10055 16333-16339 NN denotes region
T10056 16326-16329 CD denotes 1.2
T10057 16330-16332 NN denotes kb
T10058 16329-16330 HYPH denotes -
T10059 16370-16378 VBZ denotes contains
T10060 16340-16342 IN denotes of
T10061 16343-16346 DT denotes the
T10062 16361-16369 NN denotes sequence
T10063 16347-16349 NN denotes mr
T10064 16350-16351 NN denotes s
T10065 16349-16350 HYPH denotes -
T10066 16352-16360 JJ denotes upstream
T10067 16379-16384 CD denotes three
T10068 16407-16416 NNS denotes sequences
T10069 16385-16388 NN denotes Crx
T10070 16389-16396 NN denotes binding
T10071 16397-16406 NN denotes consensus
T10072 16416-16417 . denotes .
T10073 16417-16678 sentence denotes As shown in Fig. 3E, the luciferase activity was significantly upregulated when the Crx or Otx2 expression vector was co-introduced with Pro1.2k into HEK293T cells, while the luciferase activity was not altered when the Nrl expression vector was co-introduced.
T10074 16418-16420 IN denotes As
T10075 16421-16426 VBN denotes shown
T10076 16481-16492 VBN denotes upregulated
T10077 16427-16429 IN denotes in
T10078 16430-16434 NN denotes Fig.
T10079 16435-16437 NN denotes 3E
T10080 16437-16439 , denotes ,
T10081 16439-16442 DT denotes the
T10082 16454-16462 NN denotes activity
T10083 16443-16453 NN denotes luciferase
T10084 16463-16466 VBD denotes was
T10085 16467-16480 RB denotes significantly
T10086 16493-16497 WRB denotes when
T10087 16536-16549 VBN denotes co-introduced
T10088 16498-16501 DT denotes the
T10089 16525-16531 NN denotes vector
T10090 16502-16505 NN denotes Crx
T10091 16506-16508 CC denotes or
T10092 16509-16513 NN denotes Otx2
T10093 16514-16524 NN denotes expression
T10094 16532-16535 VBD denotes was
T10095 16550-16554 IN denotes with
T10096 16555-16562 NN denotes Pro1.2k
T10097 16563-16567 IN denotes into
T10098 16568-16575 NN denotes HEK293T
T10099 16576-16581 NNS denotes cells
T10100 16581-16583 , denotes ,
T10101 16583-16588 IN denotes while
T10102 16621-16628 VBN denotes altered
T10103 16589-16592 DT denotes the
T10104 16604-16612 NN denotes activity
T10105 16593-16603 NN denotes luciferase
T10106 16613-16616 VBD denotes was
T10107 16617-16620 RB denotes not
T10108 16629-16633 WRB denotes when
T10109 16664-16677 VBN denotes co-introduced
T10110 16634-16637 DT denotes the
T10111 16653-16659 NN denotes vector
T10112 16638-16641 NN denotes Nrl
T10113 16642-16652 NN denotes expression
T10114 16660-16663 VBD denotes was
T10115 16677-16678 . denotes .
T10116 16678-16830 sentence denotes A previous report suggested that the transcriptional activity of Crx is augmented with Nrl when the rhodopsin promoter was used as a reporter gene [6].
T10117 16679-16680 DT denotes A
T10118 16690-16696 NN denotes report
T10119 16681-16689 JJ denotes previous
T10120 16697-16706 VBD denotes suggested
T10121 16707-16711 IN denotes that
T10122 16751-16760 VBN denotes augmented
T10123 16712-16715 DT denotes the
T10124 16732-16740 NN denotes activity
T10125 16716-16731 JJ denotes transcriptional
T10126 16741-16743 IN denotes of
T10127 16744-16747 NN denotes Crx
T10128 16748-16750 VBZ denotes is
T10129 16761-16765 IN denotes with
T10130 16766-16769 NN denotes Nrl
T10131 16770-16774 WRB denotes when
T10132 16802-16806 VBN denotes used
T10133 16775-16778 DT denotes the
T10134 16789-16797 NN denotes promoter
T10135 16779-16788 NN denotes rhodopsin
T10136 16798-16801 VBD denotes was
T10137 16807-16809 IN denotes as
T10138 16810-16811 DT denotes a
T10139 16821-16825 NN denotes gene
T10140 16812-16820 NN denotes reporter
T10141 16826-16827 -LRB- denotes [
T10142 16827-16828 CD denotes 6
T10143 16828-16829 -RRB- denotes ]
T10144 16829-16830 . denotes .
T10145 16830-17079 sentence denotes On the other hand, our present data showed that the luciferase gene expression was not upregulated when both Crx and Nrl expression vectors were co-introduced with Pro1.2k compared to the activity when the Crx only expression vector was introduced.
T10146 16831-16833 IN denotes On
T10147 16867-16873 VBD denotes showed
T10148 16834-16837 DT denotes the
T10149 16844-16848 NN denotes hand
T10150 16838-16843 JJ denotes other
T10151 16848-16850 , denotes ,
T10152 16850-16853 PRP$ denotes our
T10153 16862-16866 NNS denotes data
T10154 16854-16861 JJ denotes present
T10155 16874-16878 IN denotes that
T10156 16918-16929 VBN denotes upregulated
T10157 16879-16882 DT denotes the
T10158 16899-16909 NN denotes expression
T10159 16883-16893 NN denotes luciferase
T10160 16894-16898 NN denotes gene
T10161 16910-16913 VBD denotes was
T10162 16914-16917 RB denotes not
T10163 16930-16934 WRB denotes when
T10164 16976-16989 VBN denotes co-introduced
T10165 16935-16939 CC denotes both
T10166 16940-16943 NN denotes Crx
T10167 16963-16970 NNS denotes vectors
T10168 16944-16947 CC denotes and
T10169 16948-16951 NN denotes Nrl
T10170 16952-16962 NN denotes expression
T10171 16971-16975 VBD denotes were
T10172 16990-16994 IN denotes with
T10173 16995-17002 NN denotes Pro1.2k
T10174 17003-17011 VBN denotes compared
T10175 17012-17014 IN denotes to
T10176 17015-17018 DT denotes the
T10177 17019-17027 NN denotes activity
T10178 17028-17032 WRB denotes when
T10179 17068-17078 VBN denotes introduced
T10180 17033-17036 DT denotes the
T10181 17057-17063 NN denotes vector
T10182 17037-17040 NN denotes Crx
T10183 17041-17045 JJ denotes only
T10184 17046-17056 NN denotes expression
T10185 17064-17067 VBD denotes was
T10186 17078-17079 . denotes .
T10187 17079-17203 sentence denotes This may be due to cell type differences because a cell type of retinal/pineal origin was not used in our luciferase assay.
T10188 17080-17084 DT denotes This
T10189 17089-17091 VB denotes be
T10190 17085-17088 MD denotes may
T10191 17092-17095 IN denotes due
T10192 17096-17098 IN denotes to
T10193 17099-17103 NN denotes cell
T10194 17104-17108 NN denotes type
T10195 17109-17120 NNS denotes differences
T10196 17121-17128 IN denotes because
T10197 17174-17178 VBN denotes used
T10198 17129-17130 DT denotes a
T10199 17136-17140 NN denotes type
T10200 17131-17135 NN denotes cell
T10201 17141-17143 IN denotes of
T10202 17144-17151 JJ denotes retinal
T10203 17152-17158 JJ denotes pineal
T10204 17151-17152 HYPH denotes /
T10205 17159-17165 NN denotes origin
T10206 17166-17169 VBD denotes was
T10207 17170-17173 RB denotes not
T10208 17179-17181 IN denotes in
T10209 17182-17185 PRP$ denotes our
T10210 17197-17202 NN denotes assay
T10211 17186-17196 NN denotes luciferase
T10212 17202-17203 . denotes .
T10213 17203-17349 sentence denotes In addition, our present data showed that Otx2, which is reported to have the same binding consensus as Crx, also transactivated mr-s expression.
T10214 17204-17206 IN denotes In
T10215 17234-17240 VBD denotes showed
T10216 17207-17215 NN denotes addition
T10217 17215-17217 , denotes ,
T10218 17217-17220 PRP$ denotes our
T10219 17229-17233 NNS denotes data
T10220 17221-17228 JJ denotes present
T10221 17241-17245 IN denotes that
T10222 17318-17332 VBN denotes transactivated
T10223 17246-17250 NN denotes Otx2
T10224 17250-17252 , denotes ,
T10225 17252-17257 WDT denotes which
T10226 17261-17269 VBN denotes reported
T10227 17258-17260 VBZ denotes is
T10228 17270-17272 TO denotes to
T10229 17273-17277 VB denotes have
T10230 17278-17281 DT denotes the
T10231 17295-17304 NN denotes consensus
T10232 17282-17286 JJ denotes same
T10233 17287-17294 NN denotes binding
T10234 17305-17307 IN denotes as
T10235 17308-17311 NN denotes Crx
T10236 17311-17313 , denotes ,
T10237 17313-17317 RB denotes also
T10238 17333-17335 NN denotes mr
T10239 17336-17337 NN denotes s
T10240 17335-17336 HYPH denotes -
T10241 17338-17348 NN denotes expression
T10242 17348-17349 . denotes .
T10243 17349-17432 sentence denotes As shown in Fig. 2A–F, the expression pattern of mr-s correlates with that of Crx.
T10244 17350-17352 IN denotes As
T10245 17353-17358 VBN denotes shown
T10246 17404-17414 VBZ denotes correlates
T10247 17359-17361 IN denotes in
T10248 17362-17366 NN denotes Fig.
T10249 17367-17369 NN denotes 2A
T10250 17369-17370 SYM denotes –
T10251 17370-17371 NN denotes F
T10252 17371-17373 , denotes ,
T10253 17373-17376 DT denotes the
T10254 17388-17395 NN denotes pattern
T10255 17377-17387 NN denotes expression
T10256 17396-17398 IN denotes of
T10257 17399-17401 NN denotes mr
T10258 17402-17403 NN denotes s
T10259 17401-17402 HYPH denotes -
T10260 17415-17419 IN denotes with
T10261 17420-17424 DT denotes that
T10262 17425-17427 IN denotes of
T10263 17428-17431 NN denotes Crx
T10264 17431-17432 . denotes .
T10265 17432-17537 sentence denotes In contrast, the transcripts of Otx2 are mainly detected in the photoreceptor layer at embryonic stages.
T10266 17433-17435 IN denotes In
T10267 17481-17489 VBN denotes detected
T10268 17436-17444 NN denotes contrast
T10269 17444-17446 , denotes ,
T10270 17446-17449 DT denotes the
T10271 17450-17461 NNS denotes transcripts
T10272 17462-17464 IN denotes of
T10273 17465-17469 NN denotes Otx2
T10274 17470-17473 VBP denotes are
T10275 17474-17480 RB denotes mainly
T10276 17490-17492 IN denotes in
T10277 17493-17496 DT denotes the
T10278 17511-17516 NN denotes layer
T10279 17497-17510 NN denotes photoreceptor
T10280 17517-17519 IN denotes at
T10281 17520-17529 JJ denotes embryonic
T10282 17530-17536 NNS denotes stages
T10283 17536-17537 . denotes .
T10284 17537-17622 sentence denotes Therefore, we concluded that mr-s transcription is directly regulated mainly by Crx.
T10285 17538-17547 RB denotes Therefore
T10286 17552-17561 VBD denotes concluded
T10287 17547-17549 , denotes ,
T10288 17549-17551 PRP denotes we
T10289 17562-17566 IN denotes that
T10290 17598-17607 VBN denotes regulated
T10291 17567-17569 NN denotes mr
T10292 17570-17571 NN denotes s
T10293 17569-17570 HYPH denotes -
T10294 17572-17585 NN denotes transcription
T10295 17586-17588 VBZ denotes is
T10296 17589-17597 RB denotes directly
T10297 17608-17614 RB denotes mainly
T10298 17615-17617 IN denotes by
T10299 17618-17621 NN denotes Crx
T10300 17621-17622 . denotes .
T10301 17622-17769 sentence denotes We also constructed reporter vectors in which mutations were introduced at the three Crx binding sites (Fig. 3D, mut1259, mut198, mut72, mut all).
T10302 17623-17625 PRP denotes We
T10303 17631-17642 VBD denotes constructed
T10304 17626-17630 RB denotes also
T10305 17643-17651 NN denotes reporter
T10306 17652-17659 NNS denotes vectors
T10307 17660-17662 IN denotes in
T10308 17684-17694 VBN denotes introduced
T10309 17663-17668 WDT denotes which
T10310 17669-17678 NNS denotes mutations
T10311 17679-17683 VBD denotes were
T10312 17695-17697 IN denotes at
T10313 17698-17701 DT denotes the
T10314 17720-17725 NNS denotes sites
T10315 17702-17707 CD denotes three
T10316 17708-17711 NN denotes Crx
T10317 17712-17719 NN denotes binding
T10318 17726-17727 -LRB- denotes (
T10319 17736-17743 NN denotes mut1259
T10320 17727-17731 NN denotes Fig.
T10321 17732-17734 NN denotes 3D
T10322 17734-17736 , denotes ,
T10323 17743-17745 , denotes ,
T10324 17745-17751 NN denotes mut198
T10325 17751-17753 , denotes ,
T10326 17753-17758 NN denotes mut72
T10327 17758-17760 , denotes ,
T10328 17760-17763 NN denotes mut
T10329 17764-17767 NN denotes all
T10330 17767-17768 -RRB- denotes )
T10331 17768-17769 . denotes .
T10332 17769-17883 sentence denotes Then the Crx expression vector was co-introduced with mut1259, mut198, mut72 and mut all, respectively (Fig. 3F).
T10333 17770-17774 RB denotes Then
T10334 17805-17818 VBN denotes co-introduced
T10335 17775-17778 DT denotes the
T10336 17794-17800 NN denotes vector
T10337 17779-17782 NN denotes Crx
T10338 17783-17793 NN denotes expression
T10339 17801-17804 VBD denotes was
T10340 17819-17823 IN denotes with
T10341 17824-17831 NN denotes mut1259
T10342 17831-17833 , denotes ,
T10343 17833-17839 NN denotes mut198
T10344 17839-17841 , denotes ,
T10345 17841-17846 NN denotes mut72
T10346 17847-17850 CC denotes and
T10347 17851-17854 NN denotes mut
T10348 17855-17858 NN denotes all
T10349 17858-17860 , denotes ,
T10350 17860-17872 RB denotes respectively
T10351 17873-17874 -LRB- denotes (
T10352 17879-17881 NN denotes 3F
T10353 17874-17878 NN denotes Fig.
T10354 17881-17882 -RRB- denotes )
T10355 17882-17883 . denotes .
T10356 17883-17978 sentence denotes The transactivaton activity by Crx was clearly reduced when mut198 or mut72 was co-introduced.
T10357 17884-17887 DT denotes The
T10358 17903-17911 NN denotes activity
T10359 17888-17902 NN denotes transactivaton
T10360 17931-17938 VBN denotes reduced
T10361 17912-17914 IN denotes by
T10362 17915-17918 NN denotes Crx
T10363 17919-17922 VBD denotes was
T10364 17923-17930 RB denotes clearly
T10365 17939-17943 WRB denotes when
T10366 17964-17977 VBN denotes co-introduced
T10367 17944-17950 NN denotes mut198
T10368 17951-17953 CC denotes or
T10369 17954-17959 NN denotes mut72
T10370 17960-17963 VBD denotes was
T10371 17977-17978 . denotes .
T10372 17978-18083 sentence denotes On the other hand, when mut1259 was co-transfected, the transactivation activity by Crx was not altered.
T10373 17979-17981 IN denotes On
T10374 18075-18082 VBN denotes altered
T10375 17982-17985 DT denotes the
T10376 17992-17996 NN denotes hand
T10377 17986-17991 JJ denotes other
T10378 17996-17998 , denotes ,
T10379 17998-18002 WRB denotes when
T10380 18015-18029 VBN denotes co-transfected
T10381 18003-18010 NN denotes mut1259
T10382 18011-18014 VBD denotes was
T10383 18029-18031 , denotes ,
T10384 18031-18034 DT denotes the
T10385 18051-18059 NN denotes activity
T10386 18035-18050 NN denotes transactivation
T10387 18060-18062 IN denotes by
T10388 18063-18066 NN denotes Crx
T10389 18067-18070 VBD denotes was
T10390 18071-18074 RB denotes not
T10391 18082-18083 . denotes .
T10392 18083-18265 sentence denotes These results suggest that the Crx binding sites 72-bp and 198-bp upstream from the transcription initiation site are crucial for the direct regulation of mr-s transcription by Crx.
T10393 18084-18089 DT denotes These
T10394 18090-18097 NNS denotes results
T10395 18098-18105 VBP denotes suggest
T10396 18106-18110 IN denotes that
T10397 18198-18201 VBP denotes are
T10398 18111-18114 DT denotes the
T10399 18127-18132 NNS denotes sites
T10400 18115-18118 NN denotes Crx
T10401 18119-18126 NN denotes binding
T10402 18133-18135 CD denotes 72
T10403 18136-18138 NN denotes bp
T10404 18135-18136 HYPH denotes -
T10405 18150-18158 RB denotes upstream
T10406 18139-18142 CC denotes and
T10407 18143-18146 CD denotes 198
T10408 18147-18149 NN denotes bp
T10409 18146-18147 HYPH denotes -
T10410 18159-18163 IN denotes from
T10411 18164-18167 DT denotes the
T10412 18193-18197 NN denotes site
T10413 18168-18181 NN denotes transcription
T10414 18182-18192 NN denotes initiation
T10415 18202-18209 JJ denotes crucial
T10416 18210-18213 IN denotes for
T10417 18214-18217 DT denotes the
T10418 18225-18235 NN denotes regulation
T10419 18218-18224 JJ denotes direct
T10420 18236-18238 IN denotes of
T10421 18239-18241 NN denotes mr
T10422 18242-18243 NN denotes s
T10423 18241-18242 HYPH denotes -
T10424 18244-18257 NN denotes transcription
T10425 18258-18260 IN denotes by
T10426 18261-18264 NN denotes Crx
T10427 18264-18265 . denotes .
T12462 18267-18271 NN denotes Self
T12463 18272-18283 NN denotes association
T12464 18271-18272 HYPH denotes -
T12465 18284-18286 IN denotes of
T12466 18287-18289 NN denotes mr
T12467 18290-18291 NN denotes s
T12468 18289-18290 HYPH denotes -
T12469 18292-18299 NN denotes protein
T12470 18299-18381 sentence denotes SAM domains are known to function as protein-protein interaction modules [15-17].
T12471 18300-18303 NN denotes SAM
T12472 18304-18311 NNS denotes domains
T12473 18316-18321 VBN denotes known
T12474 18312-18315 VBP denotes are
T12475 18322-18324 TO denotes to
T12476 18325-18333 VB denotes function
T12477 18334-18336 IN denotes as
T12478 18337-18344 NN denotes protein
T12479 18345-18352 NN denotes protein
T12480 18344-18345 HYPH denotes -
T12481 18365-18372 NNS denotes modules
T12482 18353-18364 NN denotes interaction
T12483 18373-18374 -LRB- denotes [
T12484 18374-18376 CD denotes 15
T12485 18376-18377 SYM denotes -
T12486 18377-18379 CD denotes 17
T12487 18379-18380 -RRB- denotes ]
T12488 18380-18381 . denotes .
T12489 18381-18527 sentence denotes Although SAM domains can bind to various non-SAM domain-containing proteins, many homo-SAM and hetero-SAM domain interactions have been reported.
T12490 18382-18390 IN denotes Although
T12491 18407-18411 VB denotes bind
T12492 18391-18394 NN denotes SAM
T12493 18395-18402 NNS denotes domains
T12494 18403-18406 MD denotes can
T12495 18518-18526 VBN denotes reported
T12496 18412-18414 IN denotes to
T12497 18415-18422 JJ denotes various
T12498 18449-18457 NN denotes proteins
T12499 18423-18430 JJ denotes non-SAM
T12500 18431-18437 NN denotes domain
T12501 18438-18448 VBG denotes containing
T12502 18437-18438 HYPH denotes -
T12503 18457-18459 , denotes ,
T12504 18459-18463 JJ denotes many
T12505 18495-18507 NNS denotes interactions
T12506 18464-18472 NN denotes homo-SAM
T12507 18473-18476 CC denotes and
T12508 18477-18487 NN denotes hetero-SAM
T12509 18488-18494 NN denotes domain
T12510 18508-18512 VBP denotes have
T12511 18513-18517 VBN denotes been
T12512 18526-18527 . denotes .
T12513 18527-18728 sentence denotes To investigate whether the SAM domain of the mr-s protein can also function as a protein-protein interaction module, we performed yeast two-hybrid screening using full-length mr-s protein as the bait.
T12514 18528-18530 TO denotes To
T12515 18531-18542 VB denotes investigate
T12516 18648-18657 VBD denotes performed
T12517 18543-18550 IN denotes whether
T12518 18595-18603 VB denotes function
T12519 18551-18554 DT denotes the
T12520 18559-18565 NN denotes domain
T12521 18555-18558 NN denotes SAM
T12522 18566-18568 IN denotes of
T12523 18569-18572 DT denotes the
T12524 18578-18585 NN denotes protein
T12525 18573-18575 NN denotes mr
T12526 18576-18577 NN denotes s
T12527 18575-18576 HYPH denotes -
T12528 18586-18589 MD denotes can
T12529 18590-18594 RB denotes also
T12530 18604-18606 IN denotes as
T12531 18607-18608 DT denotes a
T12532 18637-18643 NN denotes module
T12533 18609-18616 NN denotes protein
T12534 18617-18624 NN denotes protein
T12535 18616-18617 HYPH denotes -
T12536 18625-18636 NN denotes interaction
T12537 18643-18645 , denotes ,
T12538 18645-18647 PRP denotes we
T12539 18658-18663 NN denotes yeast
T12540 18675-18684 NN denotes screening
T12541 18664-18667 CD denotes two
T12542 18668-18674 NN denotes hybrid
T12543 18667-18668 HYPH denotes -
T12544 18685-18690 VBG denotes using
T12545 18691-18695 JJ denotes full
T12546 18696-18702 NN denotes length
T12547 18695-18696 HYPH denotes -
T12548 18708-18715 NN denotes protein
T12549 18703-18705 NN denotes mr
T12550 18706-18707 NN denotes s
T12551 18705-18706 HYPH denotes -
T12552 18716-18718 IN denotes as
T12553 18719-18722 DT denotes the
T12554 18723-18727 NN denotes bait
T12555 18727-18728 . denotes .
T12556 18728-18887 sentence denotes Using this bait, we screened the transcriptional activator fusion protein library in which mouse P0-P3 retinal cDNAs were fused to the GAL4 activation domain.
T12557 18729-18734 VBG denotes Using
T12558 18749-18757 VBD denotes screened
T12559 18735-18739 DT denotes this
T12560 18740-18744 NN denotes bait
T12561 18744-18746 , denotes ,
T12562 18746-18748 PRP denotes we
T12563 18758-18761 DT denotes the
T12564 18803-18810 NN denotes library
T12565 18762-18777 JJ denotes transcriptional
T12566 18778-18787 NN denotes activator
T12567 18795-18802 NN denotes protein
T12568 18788-18794 NN denotes fusion
T12569 18811-18813 IN denotes in
T12570 18851-18856 VBN denotes fused
T12571 18814-18819 WDT denotes which
T12572 18820-18825 NN denotes mouse
T12573 18840-18845 NNS denotes cDNAs
T12574 18826-18828 NN denotes P0
T12575 18828-18829 SYM denotes -
T12576 18829-18831 NN denotes P3
T12577 18832-18839 JJ denotes retinal
T12578 18846-18850 VBD denotes were
T12579 18857-18859 IN denotes to
T12580 18860-18863 DT denotes the
T12581 18880-18886 NN denotes domain
T12582 18864-18868 NN denotes GAL4
T12583 18869-18879 NN denotes activation
T12584 18886-18887 . denotes .
T12585 18887-19000 sentence denotes The most frequent positive clones (5 out of 28) were cDNA fragments containing the SAM domain of mr-s (Fig. 4A).
T12586 18888-18891 DT denotes The
T12587 18915-18921 NNS denotes clones
T12588 18892-18896 RBS denotes most
T12589 18897-18905 JJ denotes frequent
T12590 18906-18914 JJ denotes positive
T12591 18936-18940 VBD denotes were
T12592 18922-18923 -LRB- denotes (
T12593 18932-18934 CD denotes 28
T12594 18923-18924 CD denotes 5
T12595 18925-18928 IN denotes out
T12596 18929-18931 IN denotes of
T12597 18934-18935 -RRB- denotes )
T12598 18941-18945 NN denotes cDNA
T12599 18946-18955 NNS denotes fragments
T12600 18956-18966 VBG denotes containing
T12601 18967-18970 DT denotes the
T12602 18975-18981 NN denotes domain
T12603 18971-18974 NN denotes SAM
T12604 18982-18984 IN denotes of
T12605 18985-18987 NN denotes mr
T12606 18988-18989 NN denotes s
T12607 18987-18988 HYPH denotes -
T12608 18990-18991 -LRB- denotes (
T12609 18996-18998 NN denotes 4A
T12610 18991-18995 NN denotes Fig.
T12611 18998-18999 -RRB- denotes )
T12612 18999-19000 . denotes .
T12613 19000-19103 sentence denotes This result strongly suggests that mr-s protein self-associates through SAM domain-containing regions.
T12614 19001-19005 DT denotes This
T12615 19006-19012 NN denotes result
T12616 19022-19030 VBZ denotes suggests
T12617 19013-19021 RB denotes strongly
T12618 19031-19035 IN denotes that
T12619 19054-19064 VBZ denotes associates
T12620 19036-19038 NN denotes mr
T12621 19039-19040 NN denotes s
T12622 19038-19039 HYPH denotes -
T12623 19041-19048 NN denotes protein
T12624 19049-19053 NN denotes self
T12625 19053-19054 HYPH denotes -
T12626 19065-19072 IN denotes through
T12627 19073-19076 NN denotes SAM
T12628 19077-19083 NN denotes domain
T12629 19084-19094 VBG denotes containing
T12630 19083-19084 HYPH denotes -
T12631 19095-19102 NNS denotes regions
T12632 19102-19103 . denotes .
T12633 19103-19175 sentence denotes We then directly tested this self-association of mr-s protein in yeast.
T12634 19104-19106 PRP denotes We
T12635 19121-19127 VBN denotes tested
T12636 19107-19111 RB denotes then
T12637 19112-19120 RB denotes directly
T12638 19128-19132 DT denotes this
T12639 19138-19149 NN denotes association
T12640 19133-19137 NN denotes self
T12641 19137-19138 HYPH denotes -
T12642 19150-19152 IN denotes of
T12643 19153-19155 NN denotes mr
T12644 19156-19157 NN denotes s
T12645 19155-19156 HYPH denotes -
T12646 19158-19165 NN denotes protein
T12647 19166-19168 IN denotes in
T12648 19169-19174 NN denotes yeast
T12649 19174-19175 . denotes .
T12650 19175-19328 sentence denotes We fused full-length or truncated portions of the mr-s protein to the DNA-binding domain of the yeast transcription factor GAL4 to make bait constructs.
T12651 19176-19178 PRP denotes We
T12652 19179-19184 VBD denotes fused
T12653 19185-19189 JJ denotes full
T12654 19190-19196 NN denotes length
T12655 19189-19190 HYPH denotes -
T12656 19210-19218 NNS denotes portions
T12657 19197-19199 CC denotes or
T12658 19200-19209 VBN denotes truncated
T12659 19219-19221 IN denotes of
T12660 19222-19225 DT denotes the
T12661 19231-19238 NN denotes protein
T12662 19226-19228 NN denotes mr
T12663 19229-19230 NN denotes s
T12664 19228-19229 HYPH denotes -
T12665 19239-19241 IN denotes to
T12666 19242-19245 DT denotes the
T12667 19258-19264 NN denotes domain
T12668 19246-19249 NN denotes DNA
T12669 19250-19257 VBG denotes binding
T12670 19249-19250 HYPH denotes -
T12671 19265-19267 IN denotes of
T12672 19268-19271 DT denotes the
T12673 19292-19298 NN denotes factor
T12674 19272-19277 NN denotes yeast
T12675 19278-19291 NN denotes transcription
T12676 19299-19303 NN denotes GAL4
T12677 19304-19306 TO denotes to
T12678 19307-19311 VB denotes make
T12679 19312-19316 NN denotes bait
T12680 19317-19327 NNS denotes constructs
T12681 19327-19328 . denotes .
T12682 19328-19472 sentence denotes We fused full-length or truncated portions of the mr-s protein to the GAL4 transcriptional activation domain to make prey constructs (Fig. 4B).
T12683 19329-19331 PRP denotes We
T12684 19332-19337 VBD denotes fused
T12685 19338-19342 JJ denotes full
T12686 19343-19349 NN denotes length
T12687 19342-19343 HYPH denotes -
T12688 19363-19371 NNS denotes portions
T12689 19350-19352 CC denotes or
T12690 19353-19362 VBN denotes truncated
T12691 19372-19374 IN denotes of
T12692 19375-19378 DT denotes the
T12693 19384-19391 NN denotes protein
T12694 19379-19381 NN denotes mr
T12695 19382-19383 NN denotes s
T12696 19381-19382 HYPH denotes -
T12697 19392-19394 IN denotes to
T12698 19395-19398 DT denotes the
T12699 19431-19437 NN denotes domain
T12700 19399-19403 NN denotes GAL4
T12701 19420-19430 NN denotes activation
T12702 19404-19419 JJ denotes transcriptional
T12703 19438-19440 TO denotes to
T12704 19441-19445 VB denotes make
T12705 19446-19450 NN denotes prey
T12706 19451-19461 NNS denotes constructs
T12707 19462-19463 -LRB- denotes (
T12708 19468-19470 NN denotes 4B
T12709 19463-19467 NN denotes Fig.
T12710 19470-19471 -RRB- denotes )
T12711 19471-19472 . denotes .
T12712 19472-19593 sentence denotes These constructs were transformed into yeast that contain a transgene with GAL4 binding sites upstream of the lacZ gene.
T12713 19473-19478 DT denotes These
T12714 19479-19489 NNS denotes constructs
T12715 19495-19506 VBN denotes transformed
T12716 19490-19494 VBD denotes were
T12717 19507-19511 IN denotes into
T12718 19512-19517 NN denotes yeast
T12719 19518-19522 WDT denotes that
T12720 19523-19530 VBP denotes contain
T12721 19531-19532 DT denotes a
T12722 19533-19542 NN denotes transgene
T12723 19543-19547 IN denotes with
T12724 19548-19552 NN denotes GAL4
T12725 19553-19560 NN denotes binding
T12726 19561-19566 NNS denotes sites
T12727 19567-19575 RB denotes upstream
T12728 19576-19578 IN denotes of
T12729 19579-19582 DT denotes the
T12730 19588-19592 NN denotes gene
T12731 19583-19587 NN denotes lacZ
T12732 19592-19593 . denotes .
T12733 19593-19732 sentence denotes We found that the full-length mr-s bait construct induced lacZ expression with the full-length mr-s prey construct (Fig. 4B, full × full).
T12734 19594-19596 PRP denotes We
T12735 19597-19602 VBD denotes found
T12736 19603-19607 IN denotes that
T12737 19644-19651 VBD denotes induced
T12738 19608-19611 DT denotes the
T12739 19634-19643 NN denotes construct
T12740 19612-19616 JJ denotes full
T12741 19617-19623 NN denotes length
T12742 19616-19617 HYPH denotes -
T12743 19624-19626 NN denotes mr
T12744 19627-19628 NN denotes s
T12745 19626-19627 HYPH denotes -
T12746 19629-19633 NN denotes bait
T12747 19652-19656 NN denotes lacZ
T12748 19657-19667 NN denotes expression
T12749 19668-19672 IN denotes with
T12750 19673-19676 DT denotes the
T12751 19699-19708 NN denotes construct
T12752 19677-19681 JJ denotes full
T12753 19682-19688 NN denotes length
T12754 19681-19682 HYPH denotes -
T12755 19689-19691 NN denotes mr
T12756 19692-19693 NN denotes s
T12757 19691-19692 HYPH denotes -
T12758 19694-19698 NN denotes prey
T12759 19709-19710 -LRB- denotes (
T12760 19726-19730 JJ denotes full
T12761 19710-19714 NN denotes Fig.
T12762 19715-19717 NN denotes 4B
T12763 19717-19719 , denotes ,
T12764 19719-19723 JJ denotes full
T12765 19724-19725 SYM denotes ×
T12766 19730-19731 -RRB- denotes )
T12767 19731-19732 . denotes .
T12768 19732-19885 sentence denotes The N-terminus 400 amino acid (aa) stretch of mr-s, which does not contain a SAM domain, does weakly activate transcription of lacZ (Fig. 4B, full × N).
T12769 19733-19736 DT denotes The
T12770 19768-19775 NN denotes stretch
T12771 19737-19738 NN denotes N
T12772 19739-19747 NN denotes terminus
T12773 19738-19739 HYPH denotes -
T12774 19748-19751 CD denotes 400
T12775 19752-19757 NN denotes amino
T12776 19758-19762 NN denotes acid
T12777 19763-19764 -LRB- denotes (
T12778 19764-19766 NN denotes aa
T12779 19766-19767 -RRB- denotes )
T12780 19834-19842 VB denotes activate
T12781 19776-19778 IN denotes of
T12782 19779-19781 NN denotes mr
T12783 19782-19783 NN denotes s
T12784 19781-19782 HYPH denotes -
T12785 19783-19785 , denotes ,
T12786 19785-19790 WDT denotes which
T12787 19800-19807 VB denotes contain
T12788 19791-19795 VBZ denotes does
T12789 19796-19799 RB denotes not
T12790 19808-19809 DT denotes a
T12791 19814-19820 NN denotes domain
T12792 19810-19813 NN denotes SAM
T12793 19820-19822 , denotes ,
T12795 19827-19833 RB denotes weakly
T12796 19843-19856 NN denotes transcription
T12797 19857-19859 IN denotes of
T12798 19860-19864 NN denotes lacZ
T12799 19865-19866 -LRB- denotes (
T12800 19882-19883 NN denotes N
T12801 19866-19870 NN denotes Fig.
T12802 19871-19873 NN denotes 4B
T12803 19873-19875 , denotes ,
T12804 19875-19879 JJ denotes full
T12805 19880-19881 SYM denotes ×
T12806 19883-19884 -RRB- denotes )
T12807 19884-19885 . denotes .
T12808 19885-20037 sentence denotes The N-terminus 400 aa stretch of mr-s was able to induce transcription of lacZ weakly with the same N-terminus 400 aa stretch of mr-s (Fig. 4B, N × N).
T12809 19886-19889 DT denotes The
T12810 19908-19915 NN denotes stretch
T12811 19890-19891 NN denotes N
T12812 19892-19900 NN denotes terminus
T12813 19891-19892 HYPH denotes -
T12814 19901-19904 CD denotes 400
T12815 19905-19907 NN denotes aa
T12816 19924-19927 VBD denotes was
T12817 19916-19918 IN denotes of
T12818 19919-19921 NN denotes mr
T12819 19922-19923 NN denotes s
T12820 19921-19922 HYPH denotes -
T12821 19928-19932 JJ denotes able
T12822 19933-19935 TO denotes to
T12823 19936-19942 VB denotes induce
T12824 19943-19956 NN denotes transcription
T12825 19957-19959 IN denotes of
T12826 19960-19964 NN denotes lacZ
T12827 19965-19971 RB denotes weakly
T12828 19972-19976 IN denotes with
T12829 19977-19980 DT denotes the
T12830 20004-20011 NN denotes stretch
T12831 19981-19985 JJ denotes same
T12832 19986-19987 NN denotes N
T12833 19988-19996 NN denotes terminus
T12834 19987-19988 HYPH denotes -
T12835 19997-20000 CD denotes 400
T12836 20001-20003 NN denotes aa
T12837 20012-20014 IN denotes of
T12838 20015-20017 NN denotes mr
T12839 20018-20019 NN denotes s
T12840 20017-20018 HYPH denotes -
T12841 20020-20021 -LRB- denotes (
T12842 20034-20035 NN denotes N
T12843 20021-20025 NN denotes Fig.
T12844 20026-20028 NN denotes 4B
T12845 20028-20030 , denotes ,
T12846 20030-20031 NN denotes N
T12847 20032-20033 SYM denotes ×
T12848 20035-20036 -RRB- denotes )
T12849 20036-20037 . denotes .
T12850 20037-20304 sentence denotes Although the N-terminus 400 aa mr-s protein weakly activates lacZ transcription with the same N-terminus portion, a much stronger activation of lacZ expression was observed with a C-terminus portion encoding the 391–542 aa stretch of mr-s (Fig. 4B, full × C, C × C).
T12851 20038-20046 IN denotes Although
T12852 20089-20098 VBZ denotes activates
T12853 20047-20050 DT denotes the
T12854 20074-20081 NN denotes protein
T12855 20051-20052 NN denotes N
T12856 20053-20061 NN denotes terminus
T12857 20052-20053 HYPH denotes -
T12858 20062-20065 CD denotes 400
T12859 20066-20068 NN denotes aa
T12860 20069-20071 NN denotes mr
T12861 20072-20073 NN denotes s
T12862 20071-20072 HYPH denotes -
T12863 20082-20088 RB denotes weakly
T12864 20202-20210 VBN denotes observed
T12865 20099-20103 NN denotes lacZ
T12866 20104-20117 NN denotes transcription
T12867 20118-20122 IN denotes with
T12868 20123-20126 DT denotes the
T12869 20143-20150 NN denotes portion
T12870 20127-20131 JJ denotes same
T12871 20132-20133 NN denotes N
T12872 20134-20142 NN denotes terminus
T12873 20133-20134 HYPH denotes -
T12874 20150-20152 , denotes ,
T12875 20152-20153 DT denotes a
T12876 20168-20178 NN denotes activation
T12877 20154-20158 RB denotes much
T12878 20159-20167 JJR denotes stronger
T12879 20179-20181 IN denotes of
T12880 20182-20186 NN denotes lacZ
T12881 20187-20197 NN denotes expression
T12882 20198-20201 VBD denotes was
T12883 20211-20215 IN denotes with
T12884 20216-20217 DT denotes a
T12885 20229-20236 NN denotes portion
T12886 20218-20219 NN denotes C
T12887 20220-20228 NN denotes terminus
T12888 20219-20220 HYPH denotes -
T12889 20237-20245 VBG denotes encoding
T12890 20246-20249 DT denotes the
T12891 20261-20268 NN denotes stretch
T12892 20250-20253 CD denotes 391
T12893 20258-20260 NN denotes aa
T12894 20253-20254 SYM denotes –
T12895 20254-20257 CD denotes 542
T12896 20269-20271 IN denotes of
T12897 20272-20274 NN denotes mr
T12898 20275-20276 NN denotes s
T12905 20287-20291 JJ denotes full
T12906 20292-20293 SYM denotes ×
T12907 20295-20297 , denotes ,
T12908 20297-20298 NN denotes C
T12909 20301-20302 NN denotes C
T12910 20299-20300 SYM denotes ×
T12911 20302-20303 -RRB- denotes )
T12912 20303-20304 . denotes .
T12913 20304-20471 sentence denotes Our GAL4 assay indicated that the signal when the full-length mr-s was present in both the bait and prey contexts was weaker than when isolated SAM domains were used.
T12914 20305-20308 PRP$ denotes Our
T12915 20314-20319 NN denotes assay
T12916 20309-20313 NN denotes GAL4
T12917 20320-20329 VBD denotes indicated
T12918 20330-20334 IN denotes that
T12919 20419-20422 VBD denotes was
T12920 20335-20338 DT denotes the
T12921 20339-20345 NN denotes signal
T12922 20346-20350 WRB denotes when
T12923 20372-20375 VBD denotes was
T12924 20351-20354 DT denotes the
T12925 20370-20371 NN denotes s
T12926 20355-20359 JJ denotes full
T12927 20360-20366 NN denotes length
T12928 20359-20360 HYPH denotes -
T12929 20367-20369 NN denotes mr
T12930 20369-20370 HYPH denotes -
T12931 20376-20383 JJ denotes present
T12932 20384-20386 IN denotes in
T12933 20387-20391 CC denotes both
T12934 20410-20418 NNS denotes contexts
T12935 20392-20395 DT denotes the
T12936 20396-20400 NN denotes bait
T12937 20401-20404 CC denotes and
T12938 20405-20409 NN denotes prey
T12939 20423-20429 JJR denotes weaker
T12940 20430-20434 IN denotes than
T12941 20435-20439 WRB denotes when
T12942 20466-20470 VBN denotes used
T12943 20440-20448 JJ denotes isolated
T12944 20453-20460 NNS denotes domains
T12945 20449-20452 NN denotes SAM
T12946 20461-20465 VBD denotes were
T12947 20470-20471 . denotes .
T12948 20471-20596 sentence denotes This may simply reflect the tendency for the small fusion proteins to enter the yeast nucleus and occupy GAL4 binding sites.
T12949 20472-20476 DT denotes This
T12950 20488-20495 VB denotes reflect
T12951 20477-20480 MD denotes may
T12952 20481-20487 RB denotes simply
T12953 20496-20499 DT denotes the
T12954 20500-20508 NN denotes tendency
T12955 20509-20512 IN denotes for
T12956 20542-20547 VB denotes enter
T12957 20513-20516 DT denotes the
T12958 20530-20538 NN denotes proteins
T12959 20517-20522 JJ denotes small
T12960 20523-20529 NN denotes fusion
T12961 20539-20541 TO denotes to
T12962 20548-20551 DT denotes the
T12963 20558-20565 NN denotes nucleus
T12964 20552-20557 NN denotes yeast
T12965 20566-20569 CC denotes and
T12966 20570-20576 VB denotes occupy
T12967 20577-20581 NN denotes GAL4
T12968 20582-20589 NN denotes binding
T12969 20590-20595 NNS denotes sites
T12970 20595-20596 . denotes .
T12971 20596-20729 sentence denotes Alternatively, the SAM domain may be less accessible for interaction in the full-length protein context as previously reported [39].
T12972 20597-20610 RB denotes Alternatively
T12973 20631-20633 VB denotes be
T12974 20610-20612 , denotes ,
T12975 20612-20615 DT denotes the
T12976 20620-20626 NN denotes domain
T12977 20616-20619 NN denotes SAM
T12978 20627-20630 MD denotes may
T12979 20634-20638 RBR denotes less
T12980 20639-20649 JJ denotes accessible
T12981 20650-20653 IN denotes for
T12982 20654-20665 NN denotes interaction
T12983 20666-20668 IN denotes in
T12984 20669-20672 DT denotes the
T12985 20693-20700 NN denotes context
T12986 20673-20677 JJ denotes full
T12987 20678-20684 NN denotes length
T12988 20677-20678 HYPH denotes -
T12989 20685-20692 NN denotes protein
T12990 20701-20703 IN denotes as
T12991 20715-20723 VBN denotes reported
T12992 20704-20714 RB denotes previously
T12993 20724-20725 -LRB- denotes [
T12994 20725-20727 CD denotes 39
T12995 20727-20728 -RRB- denotes ]
T12996 20728-20729 . denotes .
T12997 20729-20730 sentence denotes
T13027 21665-21666 HYPH denotes -
T13028 21698-21699 NN denotes s
T13029 21673-21677 JJ denotes full
T13030 21678-21684 NN denotes length
T13031 21677-21678 HYPH denotes -
T13032 21685-21694 VBN denotes truncated
T13033 21684-21685 HYPH denotes /
T13034 21695-21697 NN denotes mr
T13035 21697-21698 HYPH denotes -
T13036 21700-21703 CC denotes and
T13037 21704-21708 NN denotes Flag
T13038 21709-21715 VBN denotes tagged
T13039 21708-21709 HYPH denotes -
T13040 21741-21742 NN denotes s
T13041 21716-21720 JJ denotes full
T13042 21721-21727 NN denotes length
T13043 21720-21721 HYPH denotes -
T13044 21728-21737 VBN denotes truncated
T13045 21727-21728 HYPH denotes /
T13046 21738-21740 NN denotes mr
T13047 21740-21741 HYPH denotes -
T13048 21743-21744 -LRB- denotes (
T13049 21749-21751 NN denotes 5A
T13050 21744-21748 NN denotes Fig.
T13051 21751-21752 -RRB- denotes )
T13052 21752-21753 . denotes .
T13053 21753-21840 sentence denotes As a negative control, we constructed Flag-tagged Sonic hedgehog (Shh) (lane 2 and 7).
T13054 21754-21756 IN denotes As
T13055 21780-21791 VBD denotes constructed
T13056 21757-21758 DT denotes a
T13057 21768-21775 NN denotes control
T13058 21759-21767 JJ denotes negative
T13059 21775-21777 , denotes ,
T13060 21777-21779 PRP denotes we
T13061 21792-21796 NN denotes Flag
T13062 21797-21803 VBN denotes tagged
T13063 21796-21797 HYPH denotes -
T13064 21810-21818 NN denotes hedgehog
T13065 21804-21809 JJ denotes Sonic
T13066 21819-21820 -LRB- denotes (
T13067 21820-21823 NN denotes Shh
T13068 21823-21824 -RRB- denotes )
T13069 21825-21826 -LRB- denotes (
T13070 21831-21832 CD denotes 2
T13071 21826-21830 NN denotes lane
T13072 21833-21836 CC denotes and
T13073 21837-21838 CD denotes 7
T13074 21838-21839 -RRB- denotes )
T13075 21839-21840 . denotes .
T13076 21840-22131 sentence denotes In accordance with the result of the yeast two-hybrid GAL4 assay, HA-tagged full-length mr-s (full-HA) was co-immunoprecipitated with Flag-tagged full-length mr-s (Flag-mrs) and the Flag-tagged C-terminus portion containing the SAM domain (Flag-SAM), respectively (Fig 5B, lane3 and lane5).
T13077 21841-21843 IN denotes In
T13078 21948-21969 VBN denotes co-immunoprecipitated
T13079 21844-21854 NN denotes accordance
T13080 21855-21859 IN denotes with
T13081 21860-21863 DT denotes the
T13082 21864-21870 NN denotes result
T13083 21871-21873 IN denotes of
T13084 21874-21877 DT denotes the
T13085 21900-21905 NN denotes assay
T13086 21878-21883 NN denotes yeast
T13087 21884-21887 CD denotes two
T13088 21888-21894 NN denotes hybrid
T13089 21887-21888 HYPH denotes -
T13090 21895-21899 NN denotes GAL4
T13091 21905-21907 , denotes ,
T13092 21907-21909 NN denotes HA
T13093 21910-21916 VBN denotes tagged
T13094 21909-21910 HYPH denotes -
T13095 21932-21933 NN denotes s
T13096 21917-21921 JJ denotes full
T13097 21922-21928 NN denotes length
T13098 21921-21922 HYPH denotes -
T13099 21929-21931 NN denotes mr
T13100 21931-21932 HYPH denotes -
T13101 21934-21935 -LRB- denotes (
T13102 21935-21939 JJ denotes full
T13103 21940-21942 NN denotes HA
T13104 21939-21940 HYPH denotes -
T13105 21942-21943 -RRB- denotes )
T13106 21944-21947 VBD denotes was
T13107 21970-21974 IN denotes with
T13108 21975-21979 NN denotes Flag
T13109 21980-21986 VBN denotes tagged
T13110 21979-21980 HYPH denotes -
T13111 22002-22003 NN denotes s
T13112 21987-21991 JJ denotes full
T13113 21992-21998 NN denotes length
T13114 21991-21992 HYPH denotes -
T13115 21999-22001 NN denotes mr
T13116 22001-22002 HYPH denotes -
T13117 22004-22005 -LRB- denotes (
T13118 22005-22009 NN denotes Flag
T13119 22010-22013 NN denotes mrs
T13120 22009-22010 HYPH denotes -
T13121 22013-22014 -RRB- denotes )
T13122 22015-22018 CC denotes and
T13123 22019-22022 DT denotes the
T13124 22046-22053 NN denotes portion
T13125 22023-22027 NN denotes Flag
T13126 22028-22034 VBN denotes tagged
T13127 22027-22028 HYPH denotes -
T13128 22035-22036 NN denotes C
T13129 22037-22045 NN denotes terminus
T13130 22036-22037 HYPH denotes -
T13131 22054-22064 VBG denotes containing
T13132 22065-22068 DT denotes the
T13133 22073-22079 NN denotes domain
T13134 22069-22072 NN denotes SAM
T13135 22080-22081 -LRB- denotes (
T13136 22081-22085 NN denotes Flag
T13137 22086-22089 NN denotes SAM
T13138 22085-22086 HYPH denotes -
T13139 22089-22090 -RRB- denotes )
T13140 22090-22092 , denotes ,
T13141 22092-22104 RB denotes respectively
T13142 22105-22106 -LRB- denotes (
T13143 22114-22119 NN denotes lane3
T13144 22106-22109 NN denotes Fig
T13145 22110-22112 NN denotes 5B
T13146 22112-22114 , denotes ,
T13147 22120-22123 CC denotes and
T13148 22124-22129 NN denotes lane5
T13149 22129-22130 -RRB- denotes )
T13150 22130-22131 . denotes .
T13151 22131-22283 sentence denotes We also found a weak co-immunoprecipitation band in co-transfection of full-HA and Flag-tagged N-terminus portion of mr-s (Flag-ΔSAM, Fig. 5B, lane 4).
T13152 22132-22134 PRP denotes We
T13153 22140-22145 VBD denotes found
T13154 22135-22139 RB denotes also
T13155 22146-22147 DT denotes a
T13156 22176-22180 NN denotes band
T13157 22148-22152 JJ denotes weak
T13158 22153-22175 NN denotes co-immunoprecipitation
T13159 22181-22183 IN denotes in
T13160 22184-22199 NN denotes co-transfection
T13161 22200-22202 IN denotes of
T13162 22203-22207 JJ denotes full
T13163 22208-22210 NN denotes HA
T13164 22207-22208 HYPH denotes -
T13165 22211-22214 CC denotes and
T13166 22215-22219 NN denotes Flag
T13167 22220-22226 VBN denotes tagged
T13168 22219-22220 HYPH denotes -
T13169 22238-22245 NN denotes portion
T13170 22227-22228 NN denotes N
T13171 22229-22237 NN denotes terminus
T13172 22228-22229 HYPH denotes -
T13173 22246-22248 IN denotes of
T13174 22249-22251 NN denotes mr
T13175 22252-22253 NN denotes s
T13176 22251-22252 HYPH denotes -
T13177 22254-22255 -LRB- denotes (
T13178 22255-22259 NN denotes Flag
T13179 22260-22264 NN denotes ΔSAM
T13180 22259-22260 HYPH denotes -
T13181 22264-22266 , denotes ,
T13182 22275-22279 NN denotes lane
T13183 22266-22270 NN denotes Fig.
T13184 22271-22273 NN denotes 5B
T13185 22273-22275 , denotes ,
T13186 22280-22281 CD denotes 4
T13187 22281-22282 -RRB- denotes )
T13188 22282-22283 . denotes .
T13189 22283-22520 sentence denotes When ΔSAM-HA and Flag-tagged deletion mutants were co-transfected, ΔSAM-HA was co-immunoprecipitated with Flag-mrs and Flag-ΔSAM (Fig. 5B, lane 8 and lane 9), while ΔSAM-HA was not co-immunoprecipitated with Flag-SAM (Fig. 5B, lane 10).
T13190 22284-22288 WRB denotes When
T13191 22335-22349 VBN denotes co-transfected
T13192 22289-22293 NN denotes ΔSAM
T13193 22294-22296 NN denotes HA
T13194 22293-22294 HYPH denotes -
T13195 22297-22300 CC denotes and
T13196 22301-22305 NN denotes Flag
T13197 22306-22312 VBN denotes tagged
T13198 22305-22306 HYPH denotes -
T13199 22322-22329 NNS denotes mutants
T13200 22313-22321 NN denotes deletion
T13201 22330-22334 VBD denotes were
T13202 22363-22384 VBN denotes co-immunoprecipitated
T13203 22349-22351 , denotes ,
T13204 22351-22355 NN denotes ΔSAM
T13205 22356-22358 NN denotes HA
T13206 22355-22356 HYPH denotes -
T13207 22359-22362 VBD denotes was
T13208 22385-22389 IN denotes with
T13209 22390-22394 NN denotes Flag
T13210 22395-22398 NN denotes mrs
T13211 22394-22395 HYPH denotes -
T13212 22399-22402 CC denotes and
T13213 22403-22407 NN denotes Flag
T13214 22408-22412 NN denotes ΔSAM
T13215 22407-22408 HYPH denotes -
T13216 22413-22414 -LRB- denotes (
T13217 22419-22421 NN denotes 5B
T13218 22414-22418 NN denotes Fig.
T13219 22421-22423 , denotes ,
T13220 22423-22427 NN denotes lane
T13221 22428-22429 CD denotes 8
T13222 22430-22433 CC denotes and
T13223 22434-22438 NN denotes lane
T13224 22439-22440 CD denotes 9
T13225 22440-22441 -RRB- denotes )
T13226 22441-22443 , denotes ,
T13227 22443-22448 IN denotes while
T13228 22465-22486 VBN denotes co-immunoprecipitated
T13229 22449-22453 NN denotes ΔSAM
T13230 22454-22456 NN denotes HA
T13231 22453-22454 HYPH denotes -
T13232 22457-22460 VBD denotes was
T13233 22461-22464 RB denotes not
T13234 22487-22491 IN denotes with
T13235 22492-22496 NN denotes Flag
T13284 23444-23445 -RRB- denotes )
T13285 23445-23446 . denotes .
T13286 23446-23704 sentence denotes These mutations alter residues that are conserved in the SAM domain of ph and previous report indicates that these mutations of ph-SAM cause significant reduction in binding activity to the other SAM domain-containing protein, Sex comb on midleg (Scm) (41).
T13287 23447-23452 DT denotes These
T13288 23453-23462 NNS denotes mutations
T13289 23463-23468 VBP denotes alter
T13290 23469-23477 NNS denotes residues
T13291 23478-23482 WDT denotes that
T13292 23487-23496 VBN denotes conserved
T13293 23483-23486 VBP denotes are
T13294 23497-23499 IN denotes in
T13295 23500-23503 DT denotes the
T13296 23508-23514 NN denotes domain
T13297 23504-23507 NN denotes SAM
T13298 23515-23517 IN denotes of
T13299 23518-23520 NN denotes ph
T13300 23521-23524 CC denotes and
T13301 23525-23533 JJ denotes previous
T13302 23534-23540 NN denotes report
T13303 23541-23550 VBZ denotes indicates
T13304 23551-23555 IN denotes that
T13305 23582-23587 VBP denotes cause
T13306 23556-23561 DT denotes these
T13307 23562-23571 NNS denotes mutations
T13308 23572-23574 IN denotes of
T13309 23575-23577 NN denotes ph
T13310 23578-23581 NN denotes SAM
T13311 23577-23578 HYPH denotes -
T13312 23588-23599 JJ denotes significant
T13313 23600-23609 NN denotes reduction
T13314 23610-23612 IN denotes in
T13315 23613-23620 NN denotes binding
T13316 23621-23629 NN denotes activity
T13317 23630-23632 IN denotes to
T13318 23633-23636 DT denotes the
T13319 23665-23672 NN denotes protein
T13320 23637-23642 JJ denotes other
T13321 23643-23646 NN denotes SAM
T13322 23647-23653 NN denotes domain
T13323 23654-23664 VBG denotes containing
T13324 23653-23654 HYPH denotes -
T13325 23672-23674 , denotes ,
T13326 23674-23677 NN denotes Sex
T13327 23678-23682 NN denotes comb
T13328 23683-23685 IN denotes on
T13329 23686-23692 NN denotes midleg
T13330 23693-23694 -LRB- denotes (
T13331 23694-23697 NN denotes Scm
T13332 23697-23698 -RRB- denotes )
T13333 23699-23700 -LRB- denotes (
T13334 23700-23702 CD denotes 41
T13335 23702-23703 -RRB- denotes )
T13336 23703-23704 . denotes .
T13337 23704-23901 sentence denotes Based on this result, we introduced two types of site-directed mutations, which correspond to the mutations introduced in ph protein, into Flag-tagged full-length mr-s (Flag-W404A and Flag-G453A).
T13338 23705-23710 VBN denotes Based
T13339 23730-23740 VBD denotes introduced
T13340 23711-23713 IN denotes on
T13341 23714-23718 DT denotes this
T13342 23719-23725 NN denotes result
T13343 23725-23727 , denotes ,
T13344 23727-23729 PRP denotes we
T13345 23741-23744 CD denotes two
T13346 23745-23750 NNS denotes types
T13347 23751-23753 IN denotes of
T13348 23754-23758 NN denotes site
T13349 23759-23767 VBN denotes directed
T13350 23758-23759 HYPH denotes -
T13351 23768-23777 NNS denotes mutations
T13352 23777-23779 , denotes ,
T13353 23779-23784 WDT denotes which
T13354 23785-23795 VBP denotes correspond
T13355 23796-23798 IN denotes to
T13356 23799-23802 DT denotes the
T13357 23803-23812 NNS denotes mutations
T13358 23813-23823 VBN denotes introduced
T13359 23824-23826 IN denotes in
T13360 23827-23829 NN denotes ph
T13361 23830-23837 NN denotes protein
T13362 23837-23839 , denotes ,
T13363 23839-23843 IN denotes into
T13364 23844-23848 NN denotes Flag
T13365 23849-23855 VBN denotes tagged
T13366 23848-23849 HYPH denotes -
T13367 23871-23872 NN denotes s
T13368 23856-23860 JJ denotes full
T13369 23861-23867 NN denotes length
T13370 23860-23861 HYPH denotes -
T13371 23868-23870 NN denotes mr
T13372 23870-23871 HYPH denotes -
T13373 23873-23874 -LRB- denotes (
T13374 23874-23878 NN denotes Flag
T13375 23879-23884 NN denotes W404A
T13376 23878-23879 HYPH denotes -
T13377 23885-23888 CC denotes and
T13378 23889-23893 NN denotes Flag
T13379 23894-23899 NN denotes G453A
T13380 23893-23894 HYPH denotes -
T13381 23899-23900 -RRB- denotes )
T13382 23900-23901 . denotes .
T13383 23901-24059 sentence denotes We found that Flag-W404A binding activity was significantly reduced and Flag-G453A binding activity was also slightly reduced compared to Flag-mrs (Fig. 5C).
T13384 23902-23904 PRP denotes We
T13385 23905-23910 VBD denotes found
T13386 23911-23915 IN denotes that
T13387 23962-23969 VBN denotes reduced
T13388 23916-23920 NN denotes Flag
T13389 23921-23926 NN denotes W404A
T13390 23920-23921 HYPH denotes -
T13391 23927-23934 NN denotes binding
T13392 23935-23943 NN denotes activity
T13393 23944-23947 VBD denotes was
T13394 23948-23961 RB denotes significantly
T13395 23970-23973 CC denotes and
T13396 23974-23978 NN denotes Flag
T13397 23979-23984 NN denotes G453A
T13398 23978-23979 HYPH denotes -
T13399 23985-23992 NN denotes binding
T13400 23993-24001 NN denotes activity
T13401 24020-24027 VBN denotes reduced
T13402 24002-24005 VBD denotes was
T13403 24006-24010 RB denotes also
T13404 24011-24019 RB denotes slightly
T13405 24028-24036 VBN denotes compared
T13406 24037-24039 IN denotes to
T13407 24040-24044 NN denotes Flag
T13408 24045-24048 NN denotes mrs
T13409 24044-24045 HYPH denotes -
T13410 24049-24050 -LRB- denotes (
T13411 24055-24057 NN denotes 5C
T13412 24050-24054 NN denotes Fig.
T13413 24057-24058 -RRB- denotes )
T13414 24058-24059 . denotes .
T13415 24059-24258 sentence denotes These results, together with yeast two-hybrid GAL4 assay, indicate that the mr-s protein self-associates strongly through its SAM domain and weakly through the N-terminus portion lacking SAM domain.
T13416 24060-24065 DT denotes These
T13417 24066-24073 NNS denotes results
T13418 24118-24126 VBP denotes indicate
T13419 24073-24075 , denotes ,
T13420 24075-24083 RB denotes together
T13421 24084-24088 IN denotes with
T13422 24089-24094 NN denotes yeast
T13423 24111-24116 NN denotes assay
T13424 24095-24098 CD denotes two
T13425 24099-24105 NN denotes hybrid
T13426 24098-24099 HYPH denotes -
T13427 24106-24110 NN denotes GAL4
T13428 24116-24118 , denotes ,
T13429 24127-24131 IN denotes that
T13430 24154-24164 VBZ denotes associates
T13431 24132-24135 DT denotes the
T13432 24141-24148 NN denotes protein
T13433 24136-24138 NN denotes mr
T13434 24139-24140 NN denotes s
T13435 24138-24139 HYPH denotes -
T13436 24149-24153 NN denotes self
T13437 24153-24154 HYPH denotes -
T13438 24165-24173 RB denotes strongly
T13439 24174-24181 IN denotes through
T13440 24182-24185 PRP$ denotes its
T13441 24190-24196 NN denotes domain
T13442 24186-24189 NN denotes SAM
T13443 24197-24200 CC denotes and
T13444 24201-24207 RB denotes weakly
T13445 24208-24215 IN denotes through
T13446 24216-24219 DT denotes the
T13447 24231-24238 NN denotes portion
T13448 24220-24221 NN denotes N
T13449 24222-24230 NN denotes terminus
T13450 24221-24222 HYPH denotes -
T13451 24239-24246 VBG denotes lacking
T13452 24247-24250 NN denotes SAM
T13453 24251-24257 NN denotes domain
T13454 24257-24258 . denotes .
T13863 24260-24263 DT denotes The
T13864 24276-24288 NN denotes localization
T13865 24264-24275 JJ denotes subcellular
T13866 24289-24291 IN denotes of
T13867 24292-24294 NN denotes mr
T13868 24295-24296 NN denotes s
T13869 24294-24295 HYPH denotes -
T13870 24297-24304 NN denotes protein
T13871 24305-24307 IN denotes in
T13872 24308-24317 JJ denotes mammalian
T13873 24318-24323 NNS denotes cells
T13874 24323-24481 sentence denotes The putative nuclear localization signal at the N-terminus of the mr-s protein (Fig. 1A, dashed box) suggests that the mr-s protein localizes in the nucleus.
T13875 24324-24327 DT denotes The
T13876 24358-24364 NN denotes signal
T13877 24328-24336 JJ denotes putative
T13878 24337-24344 JJ denotes nuclear
T13879 24345-24357 NN denotes localization
T13880 24425-24433 VBZ denotes suggests
T13881 24365-24367 IN denotes at
T13882 24368-24371 DT denotes the
T13883 24374-24382 NN denotes terminus
T13884 24372-24373 NN denotes N
T13885 24373-24374 HYPH denotes -
T13886 24383-24385 IN denotes of
T13887 24386-24389 DT denotes the
T13888 24395-24402 NN denotes protein
T13889 24390-24392 NN denotes mr
T13890 24393-24394 NN denotes s
T13891 24392-24393 HYPH denotes -
T13892 24403-24404 -LRB- denotes (
T13893 24420-24423 NN denotes box
T13894 24404-24408 NN denotes Fig.
T13895 24409-24411 NN denotes 1A
T13896 24411-24413 , denotes ,
T13897 24413-24419 JJ denotes dashed
T13898 24423-24424 -RRB- denotes )
T13899 24434-24438 IN denotes that
T13900 24456-24465 VBZ denotes localizes
T13901 24439-24442 DT denotes the
T13902 24448-24455 NN denotes protein
T13903 24443-24445 NN denotes mr
T13904 24446-24447 NN denotes s
T13905 24445-24446 HYPH denotes -
T13906 24466-24468 IN denotes in
T13907 24469-24472 DT denotes the
T13908 24473-24480 NN denotes nucleus
T13909 24480-24481 . denotes .
T13910 24481-24623 sentence denotes To determine the subcellular localization of mr-s in mammalian cells, we introduced an HA-tagged full-length mr-s plasmid into HEK293T cells.
T13911 24482-24484 TO denotes To
T13912 24485-24494 VB denotes determine
T13913 24555-24565 VBD denotes introduced
T13914 24495-24498 DT denotes the
T13915 24511-24523 NN denotes localization
T13916 24499-24510 JJ denotes subcellular
T13917 24524-24526 IN denotes of
T13918 24527-24529 NN denotes mr
T13919 24530-24531 NN denotes s
T13920 24529-24530 HYPH denotes -
T13921 24532-24534 IN denotes in
T13922 24535-24544 JJ denotes mammalian
T13923 24545-24550 NNS denotes cells
T13924 24550-24552 , denotes ,
T13925 24552-24554 PRP denotes we
T13926 24566-24568 DT denotes an
T13927 24596-24603 NN denotes plasmid
T13928 24569-24571 NN denotes HA
T13929 24572-24578 VBN denotes tagged
T13930 24571-24572 HYPH denotes -
T13931 24579-24583 JJ denotes full
T13932 24584-24590 NN denotes length
T13933 24583-24584 HYPH denotes -
T13934 24591-24593 NN denotes mr
T13935 24594-24595 NN denotes s
T13936 24593-24594 HYPH denotes -
T13937 24604-24608 IN denotes into
T13938 24609-24616 NN denotes HEK293T
T13939 24617-24622 NNS denotes cells
T13940 24622-24623 . denotes .
T13941 24623-24735 sentence denotes Confocal microscopy showed that the mr-s protein localized mainly in the nucleus of HEK293T cells (Fig. 6A, B).
T13942 24624-24632 JJ denotes Confocal
T13943 24633-24643 NN denotes microscopy
T13944 24644-24650 VBD denotes showed
T13945 24651-24655 IN denotes that
T13946 24673-24682 VBN denotes localized
T13947 24656-24659 DT denotes the
T13948 24665-24672 NN denotes protein
T13949 24660-24662 NN denotes mr
T13950 24663-24664 NN denotes s
T13951 24662-24663 HYPH denotes -
T13952 24683-24689 RB denotes mainly
T13953 24690-24692 IN denotes in
T13954 24693-24696 DT denotes the
T13955 24697-24704 NN denotes nucleus
T13956 24705-24707 IN denotes of
T13957 24708-24715 NN denotes HEK293T
T13958 24716-24721 NNS denotes cells
T13959 24722-24723 -LRB- denotes (
T13960 24732-24733 NN denotes B
T13961 24723-24727 NN denotes Fig.
T13962 24728-24730 NN denotes 6A
T13963 24730-24732 , denotes ,
T13964 24733-24734 -RRB- denotes )
T13965 24734-24735 . denotes .
T13966 24735-24853 sentence denotes To confirm the precise localization of the mr-s protein, full-length mr-s was co-immunostained with DAPI (Fig. 6C–E).
T13967 24736-24738 TO denotes To
T13968 24739-24746 VB denotes confirm
T13969 24814-24830 VBN denotes co-immunostained
T13970 24747-24750 DT denotes the
T13971 24759-24771 NN denotes localization
T13972 24751-24758 JJ denotes precise
T13973 24772-24774 IN denotes of
T13974 24775-24778 DT denotes the
T13975 24784-24791 NN denotes protein
T13976 24779-24781 NN denotes mr
T13977 24782-24783 NN denotes s
T13978 24781-24782 HYPH denotes -
T13979 24791-24793 , denotes ,
T13980 24793-24797 JJ denotes full
T13981 24798-24804 NN denotes length
T13982 24797-24798 HYPH denotes -
T13983 24808-24809 NN denotes s
T13984 24805-24807 NN denotes mr
T13985 24807-24808 HYPH denotes -
T13986 24810-24813 VBD denotes was
T13987 24831-24835 IN denotes with
T13988 24836-24840 NN denotes DAPI
T13989 24841-24842 -LRB- denotes (
T13990 24847-24849 NN denotes 6C
T13991 24842-24846 NN denotes Fig.
T13992 24849-24850 SYM denotes –
T13993 24850-24851 NN denotes E
T13994 24851-24852 -RRB- denotes )
T13995 24852-24853 . denotes .
T13996 24853-25059 sentence denotes These data showed that the mr-s protein localizes preferentially to the nucleus in mammalian cells, supporting the idea that the mr-s protein is involved in transcriptional regulation as are ph and/or TEL.
T13997 24854-24859 DT denotes These
T13998 24860-24864 NNS denotes data
T13999 24865-24871 VBD denotes showed
T14000 24872-24876 IN denotes that
T14001 24894-24903 VBZ denotes localizes
T14002 24877-24880 DT denotes the
T14003 24886-24893 NN denotes protein
T14004 24881-24883 NN denotes mr
T14005 24884-24885 NN denotes s
T14006 24883-24884 HYPH denotes -
T14007 24904-24918 RB denotes preferentially
T14008 24919-24921 IN denotes to
T14009 24922-24925 DT denotes the
T14010 24926-24933 NN denotes nucleus
T14011 24934-24936 IN denotes in
T14012 24937-24946 JJ denotes mammalian
T14013 24947-24952 NNS denotes cells
T14014 24952-24954 , denotes ,
T14015 24954-24964 VBG denotes supporting
T14016 24965-24968 DT denotes the
T14017 24969-24973 NN denotes idea
T14018 24974-24978 IN denotes that
T14019 24999-25007 VBN denotes involved
T14020 24979-24982 DT denotes the
T14021 24988-24995 NN denotes protein
T14022 24983-24985 NN denotes mr
T14023 24986-24987 NN denotes s
T14024 24985-24986 HYPH denotes -
T14025 24996-24998 VBZ denotes is
T14026 25008-25010 IN denotes in
T14027 25011-25026 JJ denotes transcriptional
T14028 25027-25037 NN denotes regulation
T14029 25038-25040 IN denotes as
T14030 25041-25044 VBP denotes are
T14031 25045-25047 NN denotes ph
T14032 25048-25051 CC denotes and
T14033 25051-25052 HYPH denotes /
T14034 25052-25054 CC denotes or
T14035 25055-25058 NN denotes TEL
T14036 25058-25059 . denotes .
T16058 25358-25361 DT denotes The
T16059 25379-25386 NN denotes protein
T16060 25362-25366 NN denotes GAL4
T16061 25372-25378 NN denotes fusion
T16062 25366-25367 HYPH denotes -
T16063 25367-25369 NN denotes mr
T16064 25370-25371 NN denotes s
T16065 25369-25370 HYPH denotes -
T16066 25387-25396 VBZ denotes functions
T16067 25397-25399 IN denotes as
T16068 25400-25415 JJ denotes transcriptional
T16069 25416-25425 NN denotes repressor
T16070 25425-25525 sentence denotes A member of PcG proteins, ph, does not contain an obvious sequence-specific DNA binding motif (16).
T16071 25426-25427 DT denotes A
T16072 25428-25434 NN denotes member
T16073 25465-25472 VB denotes contain
T16074 25435-25437 IN denotes of
T16075 25438-25441 NN denotes PcG
T16076 25442-25450 NN denotes proteins
T16077 25450-25452 , denotes ,
T16078 25452-25454 NN denotes ph
T16079 25454-25456 , denotes ,
T16080 25456-25460 VBZ denotes does
T16081 25461-25464 RB denotes not
T16082 25473-25475 DT denotes an
T16083 25514-25519 NN denotes motif
T16084 25476-25483 JJ denotes obvious
T16085 25484-25492 NN denotes sequence
T16086 25493-25501 JJ denotes specific
T16087 25492-25493 HYPH denotes -
T16088 25502-25505 NN denotes DNA
T16089 25506-25513 VBG denotes binding
T16090 25520-25521 -LRB- denotes (
T16091 25521-25523 CD denotes 16
T16092 25523-25524 -RRB- denotes )
T16093 25524-25525 . denotes .
T16094 25525-25736 sentence denotes Ph functions as a transcriptional repressor through its polymerization and protein-protein interaction with other sequence-specific transcriptional repressors, which can form a higher order chromatin structure.
T16095 25526-25528 NN denotes Ph
T16096 25529-25538 VBZ denotes functions
T16097 25539-25541 IN denotes as
T16098 25542-25543 DT denotes a
T16099 25560-25569 NN denotes repressor
T16100 25544-25559 JJ denotes transcriptional
T16101 25570-25577 IN denotes through
T16102 25578-25581 PRP$ denotes its
T16103 25582-25596 NN denotes polymerization
T16104 25597-25600 CC denotes and
T16105 25601-25608 NN denotes protein
T16106 25609-25616 NN denotes protein
T16107 25608-25609 HYPH denotes -
T16108 25617-25628 NN denotes interaction
T16109 25629-25633 IN denotes with
T16110 25634-25639 JJ denotes other
T16111 25674-25684 NNS denotes repressors
T16112 25640-25648 NN denotes sequence
T16113 25649-25657 JJ denotes specific
T16114 25648-25649 HYPH denotes -
T16115 25658-25673 JJ denotes transcriptional
T16116 25684-25686 , denotes ,
T16117 25686-25691 WDT denotes which
T16118 25696-25700 VB denotes form
T16119 25692-25695 MD denotes can
T16120 25701-25702 DT denotes a
T16121 25726-25735 NN denotes structure
T16122 25703-25709 JJR denotes higher
T16123 25710-25715 NN denotes order
T16124 25716-25725 NN denotes chromatin
T16125 25735-25736 . denotes .
T16126 25736-25803 sentence denotes The mr-s protein also does not have an obvious DNA-binding domain.
T16127 25737-25740 DT denotes The
T16128 25746-25753 NN denotes protein
T16129 25741-25743 NN denotes mr
T16130 25744-25745 NN denotes s
T16131 25743-25744 HYPH denotes -
T16132 25768-25772 VB denotes have
T16133 25754-25758 RB denotes also
T16134 25759-25763 VBZ denotes does
T16135 25764-25767 RB denotes not
T16136 25773-25775 DT denotes an
T16137 25796-25802 NN denotes domain
T16138 25776-25783 JJ denotes obvious
T16139 25784-25787 NN denotes DNA
T16140 25788-25795 VBG denotes binding
T16141 25787-25788 HYPH denotes -
T16142 25802-25803 . denotes .
T16143 25803-25891 sentence denotes To characterize the biochemical activity of mr-s, we next performed a luciferase assay.
T16144 25804-25806 TO denotes To
T16145 25807-25819 VB denotes characterize
T16146 25862-25871 VBD denotes performed
T16147 25820-25823 DT denotes the
T16148 25836-25844 NN denotes activity
T16149 25824-25835 JJ denotes biochemical
T16150 25845-25847 IN denotes of
T16151 25848-25850 NN denotes mr
T16152 25851-25852 NN denotes s
T16153 25850-25851 HYPH denotes -
T16154 25852-25854 , denotes ,
T16155 25854-25856 PRP denotes we
T16156 25857-25861 RB denotes next
T16157 25872-25873 DT denotes a
T16158 25885-25890 NN denotes assay
T16159 25874-25884 NN denotes luciferase
T16160 25890-25891 . denotes .
T16161 25891-26021 sentence denotes We generated effector plasmids, which express various deletion constructs of mr-s fused to the GAL4 DNA-binding domain (Fig. 7A).
T16162 25892-25894 PRP denotes We
T16163 25895-25904 VBD denotes generated
T16164 25905-25913 NN denotes effector
T16165 25914-25922 NNS denotes plasmids
T16166 25922-25924 , denotes ,
T16167 25924-25929 WDT denotes which
T16168 25930-25937 VBP denotes express
T16169 25938-25945 JJ denotes various
T16170 25955-25965 NNS denotes constructs
T16171 25946-25954 NN denotes deletion
T16172 25966-25968 IN denotes of
T16173 25969-25971 NN denotes mr
T16174 25972-25973 NN denotes s
T16175 25971-25972 HYPH denotes -
T16176 25974-25979 VBN denotes fused
T16177 25980-25982 IN denotes to
T16178 25983-25986 DT denotes the
T16179 26004-26010 NN denotes domain
T16180 25987-25991 NN denotes GAL4
T16181 25992-25995 NN denotes DNA
T16182 25996-26003 VBG denotes binding
T16183 25995-25996 HYPH denotes -
T16184 26011-26012 -LRB- denotes (
T16185 26017-26019 NN denotes 7A
T16186 26012-26016 NN denotes Fig.
T16187 26019-26020 -RRB- denotes )
T16188 26020-26021 . denotes .
T16189 26021-26193 sentence denotes We first confirmed that full-length mr-s fused to GAL4 DNA binding domain (DBD-mrs) had no effect on the pGL3 promoter plasmid lacking GAL4 binding sites (data not shown).
T16190 26022-26024 PRP denotes We
T16191 26031-26040 VBD denotes confirmed
T16192 26025-26030 RB denotes first
T16193 26041-26045 IN denotes that
T16194 26106-26109 VBD denotes had
T16195 26046-26050 JJ denotes full
T16196 26051-26057 NN denotes length
T16197 26050-26051 HYPH denotes -
T16198 26061-26062 NN denotes s
T16199 26058-26060 NN denotes mr
T16200 26060-26061 HYPH denotes -
T16201 26063-26068 VBN denotes fused
T16202 26069-26071 IN denotes to
T16203 26072-26076 NN denotes GAL4
T16204 26089-26095 NN denotes domain
T16205 26077-26080 NN denotes DNA
T16206 26081-26088 NN denotes binding
T16207 26096-26097 -LRB- denotes (
T16208 26097-26100 NN denotes DBD
T16209 26101-26104 NN denotes mrs
T16210 26100-26101 HYPH denotes -
T16211 26104-26105 -RRB- denotes )
T16212 26110-26112 DT denotes no
T16213 26113-26119 NN denotes effect
T16214 26120-26122 IN denotes on
T16215 26123-26126 DT denotes the
T16216 26141-26148 NN denotes plasmid
T16217 26127-26131 NN denotes pGL3
T16218 26132-26140 NN denotes promoter
T16219 26149-26156 VBG denotes lacking
T16220 26157-26161 NN denotes GAL4
T16221 26162-26169 NN denotes binding
T16222 26170-26175 NNS denotes sites
T16223 26176-26177 -LRB- denotes (
T16224 26186-26191 VBN denotes shown
T16225 26177-26181 NNS denotes data
T16226 26182-26185 RB denotes not
T16227 26191-26192 -RRB- denotes )
T16228 26192-26193 . denotes .
T16229 26193-26340 sentence denotes When the 5xGAL4-pGL3 reporter plasmid was co-transfected, DBD-mrs repressed luciferase activity by about 90% in a dose-dependent manner (Fig. 7B).
T16230 26194-26198 WRB denotes When
T16231 26236-26250 VBN denotes co-transfected
T16232 26199-26202 DT denotes the
T16233 26224-26231 NN denotes plasmid
T16234 26203-26209 NN denotes 5xGAL4
T16235 26210-26214 NN denotes pGL3
T16236 26209-26210 HYPH denotes -
T16237 26215-26223 NN denotes reporter
T16238 26232-26235 VBD denotes was
T16239 26260-26269 VBN denotes repressed
T16240 26250-26252 , denotes ,
T16241 26252-26255 NN denotes DBD
T16242 26256-26259 NN denotes mrs
T16243 26255-26256 HYPH denotes -
T16244 26270-26280 NN denotes luciferase
T16245 26281-26289 NN denotes activity
T16246 26290-26292 IN denotes by
T16247 26293-26298 IN denotes about
T16248 26299-26301 CD denotes 90
T16249 26301-26302 NN denotes %
T16250 26303-26305 IN denotes in
T16251 26306-26307 DT denotes a
T16252 26323-26329 NN denotes manner
T16253 26308-26312 NN denotes dose
T16254 26313-26322 JJ denotes dependent
T16255 26312-26313 HYPH denotes -
T16256 26330-26331 -LRB- denotes (
T16257 26336-26338 NN denotes 7B
T16258 26331-26335 NN denotes Fig.
T16259 26338-26339 -RRB- denotes )
T16260 26339-26340 . denotes .
T16261 26340-26447 sentence denotes As a control, GAL4 DNA-binding domain (DBD) had no significant effect on the 5xGAL4-pGL3 reporter plasmid.
T16262 26341-26343 IN denotes As
T16263 26385-26388 VBD denotes had
T16264 26344-26345 DT denotes a
T16265 26346-26353 NN denotes control
T16266 26353-26355 , denotes ,
T16267 26355-26359 NN denotes GAL4
T16268 26372-26378 NN denotes domain
T16269 26360-26363 NN denotes DNA
T16270 26364-26371 VBG denotes binding
T16271 26363-26364 HYPH denotes -
T16272 26379-26380 -LRB- denotes (
T16273 26380-26383 NN denotes DBD
T16274 26383-26384 -RRB- denotes )
T16275 26389-26391 DT denotes no
T16276 26404-26410 NN denotes effect
T16277 26392-26403 JJ denotes significant
T16278 26411-26413 IN denotes on
T16279 26414-26417 DT denotes the
T16280 26439-26446 NN denotes plasmid
T16281 26418-26424 NN denotes 5xGAL4
T16282 26425-26429 NN denotes pGL3
T16283 26424-26425 HYPH denotes -
T16284 26430-26438 NN denotes reporter
T16285 26446-26447 . denotes .
T16286 26447-26573 sentence denotes In addition, we confirmed that full-length mr-s without GAL4 DBD had no effect on the same reporter plasmid (data not shown).
T16287 26448-26450 IN denotes In
T16288 26464-26473 VBD denotes confirmed
T16289 26451-26459 NN denotes addition
T16290 26459-26461 , denotes ,
T16291 26461-26463 PRP denotes we
T16292 26474-26478 IN denotes that
T16293 26513-26516 VBD denotes had
T16294 26479-26483 JJ denotes full
T16295 26484-26490 NN denotes length
T16296 26483-26484 HYPH denotes -
T16297 26494-26495 NN denotes s
T16298 26491-26493 NN denotes mr
T16299 26493-26494 HYPH denotes -
T16300 26496-26503 IN denotes without
T16301 26504-26508 NN denotes GAL4
T16302 26509-26512 NN denotes DBD
T16303 26517-26519 DT denotes no
T16304 26520-26526 NN denotes effect
T16305 26527-26529 IN denotes on
T16306 26530-26533 DT denotes the
T16307 26548-26555 NN denotes plasmid
T16308 26534-26538 JJ denotes same
T16309 26539-26547 NN denotes reporter
T16310 26556-26557 -LRB- denotes (
T16311 26566-26571 VBN denotes shown
T16312 26557-26561 NNS denotes data
T16313 26562-26565 RB denotes not
T16314 26571-26572 -RRB- denotes )
T16315 26572-26573 . denotes .
T16316 26573-26783 sentence denotes We next analyzed deletion constructs in which the N-terminus 400 aa stretch of mr-s (amino acids 1 to 400) or the C-terminus portion (amino acids 391 to 542) were fused to the GAL4 DBD (Fig. 7A, DBD-N, DBD-C).
T16317 26574-26576 PRP denotes We
T16318 26582-26590 VBD denotes analyzed
T16319 26577-26581 RB denotes next
T16320 26591-26599 NN denotes deletion
T16321 26600-26610 NNS denotes constructs
T16322 26611-26613 IN denotes in
T16323 26737-26742 VBN denotes fused
T16324 26614-26619 WDT denotes which
T16325 26620-26623 DT denotes the
T16326 26642-26649 NN denotes stretch
T16327 26624-26625 NN denotes N
T16328 26626-26634 NN denotes terminus
T16329 26625-26626 HYPH denotes -
T16330 26635-26638 CD denotes 400
T16331 26639-26641 NN denotes aa
T16332 26650-26652 IN denotes of
T16333 26653-26655 NN denotes mr
T16334 26656-26657 NN denotes s
T16335 26655-26656 HYPH denotes -
T16336 26658-26659 -LRB- denotes (
T16337 26671-26672 CD denotes 1
T16338 26659-26664 NN denotes amino
T16339 26665-26670 NNS denotes acids
T16340 26673-26675 IN denotes to
T16341 26676-26679 CD denotes 400
T16342 26679-26680 -RRB- denotes )
T16343 26681-26683 CC denotes or
T16344 26684-26687 DT denotes the
T16345 26699-26706 NN denotes portion
T16346 26688-26689 NN denotes C
T16347 26690-26698 NN denotes terminus
T16348 26689-26690 HYPH denotes -
T16349 26707-26708 -LRB- denotes (
T16350 26720-26723 CD denotes 391
T16351 26708-26713 NN denotes amino
T16352 26714-26719 NNS denotes acids
T16353 26724-26726 IN denotes to
T16354 26727-26730 CD denotes 542
T16355 26730-26731 -RRB- denotes )
T16356 26732-26736 VBD denotes were
T16357 26743-26745 IN denotes to
T16358 26746-26749 DT denotes the
T16359 26755-26758 NN denotes DBD
T16360 26750-26754 NN denotes GAL4
T16361 26759-26760 -LRB- denotes (
T16362 26765-26767 NN denotes 7A
T16363 26760-26764 NN denotes Fig.
T16364 26767-26769 , denotes ,
T16365 26769-26772 NN denotes DBD
T16366 26773-26774 NN denotes N
T16367 26772-26773 HYPH denotes -
T16368 26774-26776 , denotes ,
T16369 26776-26779 NN denotes DBD
T16370 26780-26781 NN denotes C
T16371 26779-26780 HYPH denotes -
T16372 26781-26782 -RRB- denotes )
T16373 26782-26783 . denotes .
T16374 26783-26904 sentence denotes While DBD-N had no repressive effect on this reporter activity, DBD-C repressed luciferase gene expression by about 65%.
T16375 26784-26789 IN denotes While
T16376 26796-26799 VBD denotes had
T16377 26790-26793 NN denotes DBD
T16378 26794-26795 NN denotes N
T16379 26793-26794 HYPH denotes -
T16380 26854-26863 VBD denotes repressed
T16381 26800-26802 DT denotes no
T16382 26814-26820 NN denotes effect
T16383 26803-26813 JJ denotes repressive
T16384 26821-26823 IN denotes on
T16385 26824-26828 DT denotes this
T16386 26838-26846 NN denotes activity
T16387 26829-26837 NN denotes reporter
T16388 26846-26848 , denotes ,
T16389 26848-26851 NN denotes DBD
T16390 26852-26853 NN denotes C
T16391 26851-26852 HYPH denotes -
T16392 26864-26874 NN denotes luciferase
T16393 26880-26890 NN denotes expression
T16394 26875-26879 NN denotes gene
T16395 26891-26893 IN denotes by
T16396 26894-26899 IN denotes about
T16397 26900-26902 CD denotes 65
T16398 26902-26903 NN denotes %
T16399 26903-26904 . denotes .
T16400 26904-27049 sentence denotes This result suggested to us the possibility that DBD-mrs exerts transcriptional repressive activity via self-association through its SAM domain.
T16401 26905-26909 DT denotes This
T16402 26910-26916 NN denotes result
T16403 26917-26926 VBD denotes suggested
T16404 26927-26929 IN denotes to
T16405 26930-26932 PRP denotes us
T16406 26933-26936 DT denotes the
T16407 26937-26948 NN denotes possibility
T16408 26949-26953 IN denotes that
T16409 26962-26968 VBZ denotes exerts
T16410 26954-26957 NN denotes DBD
T16411 26958-26961 NN denotes mrs
T16412 26957-26958 HYPH denotes -
T16413 26969-26984 JJ denotes transcriptional
T16414 26996-27004 NN denotes activity
T16415 26985-26995 JJ denotes repressive
T16416 27005-27008 IN denotes via
T16417 27009-27013 NN denotes self
T16418 27014-27025 NN denotes association
T16419 27013-27014 HYPH denotes -
T16420 27026-27033 IN denotes through
T16421 27034-27037 PRP$ denotes its
T16422 27042-27048 NN denotes domain
T16423 27038-27041 NN denotes SAM
T16424 27048-27049 . denotes .
T16425 27049-27277 sentence denotes To investigate whether the homophilic association of mr-s is required for transcriptional repression, two site-directed mutants, DBD-W404A and DBD-G453A, either of which may reduce self-binding ability, were analyzed (Fig. 7C).
T16426 27050-27052 TO denotes To
T16427 27053-27064 VB denotes investigate
T16428 27258-27266 VBN denotes analyzed
T16429 27065-27072 IN denotes whether
T16430 27111-27119 VBN denotes required
T16431 27073-27076 DT denotes the
T16432 27088-27099 NN denotes association
T16433 27077-27087 JJ denotes homophilic
T16434 27100-27102 IN denotes of
T16435 27103-27105 NN denotes mr
T16436 27106-27107 NN denotes s
T16437 27105-27106 HYPH denotes -
T16438 27108-27110 VBZ denotes is
T16439 27120-27123 IN denotes for
T16440 27124-27139 JJ denotes transcriptional
T16441 27140-27150 NN denotes repression
T16442 27150-27152 , denotes ,
T16443 27152-27155 CD denotes two
T16444 27170-27177 NNS denotes mutants
T16445 27156-27160 NN denotes site
T16446 27161-27169 VBN denotes directed
T16447 27160-27161 HYPH denotes -
T16448 27177-27179 , denotes ,
T16449 27179-27182 NN denotes DBD
T16450 27183-27188 NN denotes W404A
T16451 27182-27183 HYPH denotes -
T16452 27189-27192 CC denotes and
T16453 27193-27196 NN denotes DBD
T16454 27197-27202 NN denotes G453A
T16455 27196-27197 HYPH denotes -
T16456 27202-27204 , denotes ,
T16457 27204-27210 DT denotes either
T16458 27224-27230 VB denotes reduce
T16459 27211-27213 IN denotes of
T16460 27214-27219 WDT denotes which
T16461 27220-27223 MD denotes may
T16462 27231-27235 NN denotes self
T16463 27236-27243 VBG denotes binding
T16464 27235-27236 HYPH denotes -
T16465 27244-27251 NN denotes ability
T16466 27251-27253 , denotes ,
T16467 27253-27257 VBD denotes were
T16468 27267-27268 -LRB- denotes (
T16469 27273-27275 NN denotes 7C
T16470 27268-27272 NN denotes Fig.
T16471 27275-27276 -RRB- denotes )
T16472 27276-27277 . denotes .
T16473 27277-27376 sentence denotes Compared to DBD-mrs, DBD-W404A and DBD-G453A had repression activity of 72% and 87%, respectively.
T16474 27278-27286 VBN denotes Compared
T16475 27323-27326 VBD denotes had
T16476 27287-27289 IN denotes to
T16477 27290-27293 NN denotes DBD
T16478 27294-27297 NN denotes mrs
T16479 27293-27294 HYPH denotes -
T16480 27297-27299 , denotes ,
T16481 27299-27302 NN denotes DBD
T16482 27303-27308 NN denotes W404A
T16483 27302-27303 HYPH denotes -
T16484 27309-27312 CC denotes and
T16485 27313-27316 NN denotes DBD
T16486 27317-27322 NN denotes G453A
T16487 27316-27317 HYPH denotes -
T16488 27327-27337 NN denotes repression
T16489 27338-27346 NN denotes activity
T16490 27347-27349 IN denotes of
T16491 27350-27352 CD denotes 72
T16492 27352-27353 NN denotes %
T16493 27354-27357 CC denotes and
T16494 27358-27360 CD denotes 87
T16495 27360-27361 NN denotes %
T16496 27361-27363 , denotes ,
T16497 27363-27375 RB denotes respectively
T16498 27375-27376 . denotes .
T16499 27376-27563 sentence denotes While the ability of mr-s self-association partially correlates with transcriptional repressive activity, these mutants do not compromise the ability to repress transcription critically.
T16500 27377-27382 IN denotes While
T16501 27430-27440 VBZ denotes correlates
T16502 27383-27386 DT denotes the
T16503 27387-27394 NN denotes ability
T16504 27395-27397 IN denotes of
T16505 27398-27400 NN denotes mr
T16506 27401-27402 NN denotes s
T16507 27400-27401 HYPH denotes -
T16508 27408-27419 NN denotes association
T16509 27403-27407 NN denotes self
T16510 27407-27408 HYPH denotes -
T16511 27420-27429 RB denotes partially
T16512 27504-27514 VB denotes compromise
T16513 27441-27445 IN denotes with
T16514 27446-27461 JJ denotes transcriptional
T16515 27473-27481 NN denotes activity
T16516 27462-27472 JJ denotes repressive
T16517 27481-27483 , denotes ,
T16518 27483-27488 DT denotes these
T16519 27489-27496 NNS denotes mutants
T16520 27497-27499 VBP denotes do
T16521 27500-27503 RB denotes not
T16522 27515-27518 DT denotes the
T16523 27519-27526 NN denotes ability
T16524 27527-27529 TO denotes to
T16525 27530-27537 VB denotes repress
T16526 27538-27551 NN denotes transcription
T16527 27552-27562 RB denotes critically
T16528 27562-27563 . denotes .
T16529 27563-27768 sentence denotes To determine the regions of mr-s involved in transcriptional repression more precisely, the C-terminus portion was divided into two regions and each was fused to the GAL4 DBD (Fig. 7A, DBD-tail, DBD-SAM).
T16530 27564-27566 TO denotes To
T16531 27567-27576 VB denotes determine
T16532 27679-27686 VBN denotes divided
T16533 27577-27580 DT denotes the
T16534 27581-27588 NNS denotes regions
T16535 27589-27591 IN denotes of
T16536 27592-27594 NN denotes mr
T16537 27595-27596 NN denotes s
T16538 27594-27595 HYPH denotes -
T16539 27597-27605 VBN denotes involved
T16540 27606-27608 IN denotes in
T16541 27609-27624 JJ denotes transcriptional
T16542 27625-27635 NN denotes repression
T16543 27636-27640 RBR denotes more
T16544 27641-27650 RB denotes precisely
T16545 27650-27652 , denotes ,
T16546 27652-27655 DT denotes the
T16547 27667-27674 NN denotes portion
T16548 27656-27657 NN denotes C
T16549 27658-27666 NN denotes terminus
T16550 27657-27658 HYPH denotes -
T16551 27675-27678 VBD denotes was
T16552 27687-27691 IN denotes into
T16553 27692-27695 CD denotes two
T16554 27696-27703 NNS denotes regions
T16555 27704-27707 CC denotes and
T16556 27708-27712 DT denotes each
T16557 27717-27722 VBN denotes fused
T16558 27713-27716 VBD denotes was
T16559 27723-27725 IN denotes to
T16560 27726-27729 DT denotes the
T16561 27735-27738 NN denotes DBD
T16562 27730-27734 NN denotes GAL4
T16563 27739-27740 -LRB- denotes (
T16564 27763-27766 NN denotes SAM
T16565 27740-27744 NN denotes Fig.
T16566 27745-27747 NN denotes 7A
T16567 27747-27749 , denotes ,
T16568 27749-27752 NN denotes DBD
T16569 27753-27757 NN denotes tail
T16570 27752-27753 HYPH denotes -
T16571 27757-27759 , denotes ,
T16572 27759-27762 NN denotes DBD
T16573 27762-27763 HYPH denotes -
T16574 27766-27767 -RRB- denotes )
T16575 27767-27768 . denotes .
T16576 27768-27889 sentence denotes As a consequence, DBD-SAM (amino acids 384 to 462) did not have a repressive effect on the 5xGAL4-pGL3 reporter plasmid.
T16577 27769-27771 IN denotes As
T16578 27828-27832 VB denotes have
T16579 27772-27773 DT denotes a
T16580 27774-27785 NN denotes consequence
T16581 27785-27787 , denotes ,
T16582 27787-27790 NN denotes DBD
T16583 27791-27794 NN denotes SAM
T16584 27790-27791 HYPH denotes -
T16585 27795-27796 -LRB- denotes (
T16586 27808-27811 CD denotes 384
T16587 27796-27801 NN denotes amino
T16588 27802-27807 NNS denotes acids
T16589 27812-27814 IN denotes to
T16590 27815-27818 CD denotes 462
T16591 27818-27819 -RRB- denotes )
T16592 27820-27823 VBD denotes did
T16593 27824-27827 RB denotes not
T16594 27833-27834 DT denotes a
T16595 27846-27852 NN denotes effect
T16596 27835-27845 JJ denotes repressive
T16597 27853-27855 IN denotes on
T16598 27856-27859 DT denotes the
T16599 27881-27888 NN denotes plasmid
T16600 27860-27866 NN denotes 5xGAL4
T16601 27867-27871 NN denotes pGL3
T16602 27866-27867 HYPH denotes -
T16603 27872-27880 NN denotes reporter
T16604 27888-27889 . denotes .
T16605 27889-28045 sentence denotes On the other hand, luciferase activity was repressed by 55% when DBD-tail (amino acids 459 to 542) was co-transfected with this reporter plasmid (Fig. 7D).
T16606 27890-27892 IN denotes On
T16607 27933-27942 VBN denotes repressed
T16608 27893-27896 DT denotes the
T16609 27903-27907 NN denotes hand
T16610 27897-27902 JJ denotes other
T16611 27907-27909 , denotes ,
T16612 27909-27919 NN denotes luciferase
T16613 27920-27928 NN denotes activity
T16614 27929-27932 VBD denotes was
T16615 27943-27945 IN denotes by
T16616 27946-27948 CD denotes 55
T16617 27948-27949 NN denotes %
T16618 27950-27954 WRB denotes when
T16619 27993-28007 VBN denotes co-transfected
T16620 27955-27958 NN denotes DBD
T16621 27959-27963 NN denotes tail
T16622 27958-27959 HYPH denotes -
T16623 27964-27965 -LRB- denotes (
T16624 27977-27980 CD denotes 459
T16625 27965-27970 NN denotes amino
T16626 27971-27976 NNS denotes acids
T16627 27981-27983 IN denotes to
T16628 27984-27987 CD denotes 542
T16629 27987-27988 -RRB- denotes )
T16630 27989-27992 VBD denotes was
T16631 28008-28012 IN denotes with
T16632 28013-28017 DT denotes this
T16633 28027-28034 NN denotes plasmid
T16634 28018-28026 NN denotes reporter
T16635 28035-28036 -LRB- denotes (
T16636 28041-28043 NN denotes 7D
T16637 28036-28040 NN denotes Fig.
T16638 28043-28044 -RRB- denotes )
T16639 28044-28045 . denotes .
T16640 28045-28202 sentence denotes To assess the transcriptional repressive activity of mr-s in cells of retinal origin, we performed similar experiments using human Y79 retinoblastoma cells.
T16641 28046-28048 TO denotes To
T16642 28049-28055 VB denotes assess
T16643 28135-28144 VBD denotes performed
T16644 28056-28059 DT denotes the
T16645 28087-28095 NN denotes activity
T16646 28060-28075 JJ denotes transcriptional
T16647 28076-28086 JJ denotes repressive
T16648 28096-28098 IN denotes of
T16649 28099-28101 NN denotes mr
T16650 28102-28103 NN denotes s
T16651 28101-28102 HYPH denotes -
T16652 28104-28106 IN denotes in
T16653 28107-28112 NNS denotes cells
T16654 28113-28115 IN denotes of
T16655 28116-28123 JJ denotes retinal
T16656 28124-28130 NN denotes origin
T16657 28130-28132 , denotes ,
T16658 28132-28134 PRP denotes we
T16659 28145-28152 JJ denotes similar
T16660 28153-28164 NNS denotes experiments
T16661 28165-28170 VBG denotes using
T16662 28171-28176 JJ denotes human
T16663 28196-28201 NNS denotes cells
T16664 28177-28180 NN denotes Y79
T16665 28181-28195 NN denotes retinoblastoma
T16666 28201-28202 . denotes .
T16667 28202-28323 sentence denotes The results indicated that DBD-mrs also reduced luciferase activity significantly in Y79 retinoblastoma cells (Fig. 7E).
T16668 28203-28206 DT denotes The
T16669 28207-28214 NNS denotes results
T16670 28215-28224 VBD denotes indicated
T16671 28225-28229 IN denotes that
T16672 28243-28250 VBD denotes reduced
T16673 28230-28233 NN denotes DBD
T16674 28234-28237 NN denotes mrs
T16675 28233-28234 HYPH denotes -
T16676 28238-28242 RB denotes also
T16677 28251-28261 NN denotes luciferase
T16678 28262-28270 NN denotes activity
T16679 28271-28284 RB denotes significantly
T16680 28285-28287 IN denotes in
T16681 28288-28291 NN denotes Y79
T16682 28307-28312 NNS denotes cells
T16683 28292-28306 NN denotes retinoblastoma
T16684 28313-28314 -LRB- denotes (
T16685 28319-28321 NN denotes 7E
T16686 28314-28318 NN denotes Fig.
T16687 28321-28322 -RRB- denotes )
T16688 28322-28323 . denotes .
T16689 28323-28441 sentence denotes However, luciferase activity was repressed by about 30% in Y79 cells, while it was repressed by 90% in HEK293T cells.
T16690 28324-28331 RB denotes However
T16691 28357-28366 VBN denotes repressed
T16692 28331-28333 , denotes ,
T16693 28333-28343 NN denotes luciferase
T16694 28344-28352 NN denotes activity
T16695 28353-28356 VBD denotes was
T16696 28367-28369 IN denotes by
T16697 28370-28375 IN denotes about
T16698 28376-28378 CD denotes 30
T16699 28378-28379 NN denotes %
T16700 28380-28382 IN denotes in
T16701 28383-28386 NN denotes Y79
T16702 28387-28392 NNS denotes cells
T16703 28392-28394 , denotes ,
T16704 28394-28399 IN denotes while
T16705 28407-28416 VBN denotes repressed
T16706 28400-28402 PRP denotes it
T16707 28403-28406 VBD denotes was
T16708 28417-28419 IN denotes by
T16709 28420-28422 CD denotes 90
T16710 28422-28423 NN denotes %
T16711 28424-28426 IN denotes in
T16712 28427-28434 NN denotes HEK293T
T16713 28435-28440 NNS denotes cells
T16714 28440-28441 . denotes .
T16715 28441-28530 sentence denotes This might be due to the difference in transfection efficiency between these cell lines.
T16716 28442-28446 DT denotes This
T16717 28453-28455 VB denotes be
T16718 28447-28452 MD denotes might
T16719 28456-28459 IN denotes due
T16720 28460-28462 IN denotes to
T16721 28463-28466 DT denotes the
T16722 28467-28477 NN denotes difference
T16723 28478-28480 IN denotes in
T16724 28481-28493 NN denotes transfection
T16725 28494-28504 NN denotes efficiency
T16726 28505-28512 IN denotes between
T16727 28513-28518 DT denotes these
T16728 28524-28529 NNS denotes lines
T16729 28519-28523 NN denotes cell
T16730 28529-28530 . denotes .
T16731 28530-28688 sentence denotes Another possibility is that intracellular environment in Y79 cells, a retinoblastoma cell line, is insufficient for recapitulating developing photoreceptors.
T16732 28531-28538 DT denotes Another
T16733 28539-28550 NN denotes possibility
T16734 28551-28553 VBZ denotes is
T16735 28554-28558 IN denotes that
T16736 28627-28629 VBZ denotes is
T16737 28559-28572 JJ denotes intracellular
T16738 28573-28584 NN denotes environment
T16739 28585-28587 IN denotes in
T16740 28588-28591 NN denotes Y79
T16741 28592-28597 NNS denotes cells
T16742 28597-28599 , denotes ,
T16743 28599-28600 DT denotes a
T16744 28621-28625 NN denotes line
T16745 28601-28615 NN denotes retinoblastoma
T16746 28616-28620 NN denotes cell
T16747 28625-28627 , denotes ,
T16748 28630-28642 JJ denotes insufficient
T16749 28643-28646 IN denotes for
T16750 28647-28661 VBG denotes recapitulating
T16751 28662-28672 VBG denotes developing
T16752 28673-28687 NNS denotes photoreceptors
T16753 28687-28688 . denotes .
T16754 28688-28824 sentence denotes In this study, we did not address the question whether or not our in vitro data reflect native mr-s transcriptional repression in vivo.
T16755 28689-28691 IN denotes In
T16756 28715-28722 VB denotes address
T16757 28692-28696 DT denotes this
T16758 28697-28702 NN denotes study
T16759 28702-28704 , denotes ,
T16760 28704-28706 PRP denotes we
T16761 28707-28710 VBD denotes did
T16762 28711-28714 RB denotes not
T16763 28723-28726 DT denotes the
T16764 28727-28735 NN denotes question
T16765 28736-28743 IN denotes whether
T16766 28769-28776 VBP denotes reflect
T16767 28744-28746 CC denotes or
T16768 28747-28750 RB denotes not
T16769 28751-28754 PRP$ denotes our
T16770 28764-28768 NNS denotes data
T16771 28755-28757 FW denotes in
T16772 28758-28763 FW denotes vitro
T16773 28777-28783 JJ denotes native
T16774 28805-28815 NN denotes repression
T16775 28784-28786 NN denotes mr
T16776 28787-28788 NN denotes s
T16777 28786-28787 HYPH denotes -
T16778 28789-28804 JJ denotes transcriptional
T16779 28816-28818 FW denotes in
T16780 28819-28823 FW denotes vivo
T16781 28823-28824 . denotes .
T16782 28824-29001 sentence denotes However, these in vitro experiments using HEK293T and Y79 cells strongly support our hypothesis that mr-s functions as a transcriptional repressor in developing photoreceptors.
T16783 28825-28832 RB denotes However
T16784 28898-28905 VBP denotes support
T16785 28832-28834 , denotes ,
T16786 28834-28839 DT denotes these
T16787 28849-28860 NNS denotes experiments
T16788 28840-28842 FW denotes in
T16789 28843-28848 FW denotes vitro
T16790 28861-28866 VBG denotes using
T16791 28867-28874 NN denotes HEK293T
T16792 28883-28888 NNS denotes cells
T16793 28875-28878 CC denotes and
T16794 28879-28882 NN denotes Y79
T16795 28889-28897 RB denotes strongly
T16796 28906-28909 PRP$ denotes our
T16797 28910-28920 NN denotes hypothesis
T16798 28921-28925 IN denotes that
T16799 28931-28940 VBZ denotes functions
T16800 28926-28928 NN denotes mr
T16801 28929-28930 NN denotes s
T16802 28928-28929 HYPH denotes -
T16803 28941-28943 IN denotes as
T16804 28944-28945 DT denotes a
T16805 28962-28971 NN denotes repressor
T16806 28946-28961 JJ denotes transcriptional
T16807 28972-28974 IN denotes in
T16808 28975-28985 VBG denotes developing
T16809 28986-29000 NNS denotes photoreceptors
T16810 29000-29001 . denotes .
T16811 29001-29209 sentence denotes Our results suggested that the C-terminal region of mr-s (amino acids 463 to 542) is required for transcriptional repression of mr-s and the SAM domain appears to be dispensable for this repressive activity.
T16812 29002-29005 PRP$ denotes Our
T16813 29006-29013 NNS denotes results
T16814 29014-29023 VBD denotes suggested
T16815 29024-29028 IN denotes that
T16816 29087-29095 VBN denotes required
T16817 29029-29032 DT denotes the
T16818 29044-29050 NN denotes region
T16819 29033-29034 NN denotes C
T16820 29035-29043 JJ denotes terminal
T16821 29034-29035 HYPH denotes -
T16822 29051-29053 IN denotes of
T16823 29054-29056 NN denotes mr
T16824 29057-29058 NN denotes s
T16825 29056-29057 HYPH denotes -
T16826 29059-29060 -LRB- denotes (
T16827 29072-29075 CD denotes 463
T16828 29060-29065 NN denotes amino
T16829 29066-29071 NNS denotes acids
T16830 29076-29078 IN denotes to
T16831 29079-29082 CD denotes 542
T16832 29082-29083 -RRB- denotes )
T16833 29084-29086 VBZ denotes is
T16834 29096-29099 IN denotes for
T16835 29100-29115 JJ denotes transcriptional
T16836 29116-29126 NN denotes repression
T16837 29127-29129 IN denotes of
T16838 29130-29132 NN denotes mr
T16839 29133-29134 NN denotes s
T16840 29132-29133 HYPH denotes -
T16841 29135-29138 CC denotes and
T16842 29139-29142 DT denotes the
T16843 29147-29153 NN denotes domain
T16844 29143-29146 NN denotes SAM
T16845 29154-29161 VBZ denotes appears
T16846 29162-29164 TO denotes to
T16847 29165-29167 VB denotes be
T16848 29168-29179 JJ denotes dispensable
T16849 29180-29183 IN denotes for
T16850 29184-29188 DT denotes this
T16851 29200-29208 NN denotes activity
T16852 29189-29199 JJ denotes repressive
T16853 29208-29209 . denotes .
T16854 29209-29291 sentence denotes This C-terminal region of mouse mr-s is highly conserved among species (Fig. 7F).
T16855 29210-29214 DT denotes This
T16856 29226-29232 NN denotes region
T16857 29215-29216 NN denotes C
T16858 29217-29225 JJ denotes terminal
T16859 29216-29217 HYPH denotes -
T16860 29257-29266 VBN denotes conserved
T16861 29233-29235 IN denotes of
T16862 29236-29241 NN denotes mouse
T16863 29245-29246 NN denotes s
T16864 29242-29244 NN denotes mr
T16865 29244-29245 HYPH denotes -
T16866 29247-29249 VBZ denotes is
T16867 29250-29256 RB denotes highly
T16868 29267-29272 IN denotes among
T16869 29273-29280 NNS denotes species
T16870 29281-29282 -LRB- denotes (
T16871 29287-29289 NN denotes 7F
T16872 29282-29286 NN denotes Fig.
T16873 29289-29290 -RRB- denotes )
T16874 29290-29291 . denotes .
T16875 29291-29414 sentence denotes The sequence identity of the region was 93%, 73%, 41% and 40% for rat, human, chick and zebrafish, respectively (Fig. 1D).
T16876 29292-29295 DT denotes The
T16877 29305-29313 NN denotes identity
T16878 29296-29304 NN denotes sequence
T16879 29328-29331 VBD denotes was
T16880 29314-29316 IN denotes of
T16881 29317-29320 DT denotes the
T16882 29321-29327 NN denotes region
T16883 29332-29334 CD denotes 93
T16884 29334-29335 NN denotes %
T16885 29335-29337 , denotes ,
T16886 29337-29339 CD denotes 73
T16887 29339-29340 NN denotes %
T16888 29340-29342 , denotes ,
T16889 29342-29344 CD denotes 41
T16890 29344-29345 NN denotes %
T16891 29346-29349 CC denotes and
T16892 29350-29352 CD denotes 40
T16893 29352-29353 NN denotes %
T16894 29354-29357 IN denotes for
T16895 29358-29361 NN denotes rat
T16896 29361-29363 , denotes ,
T16897 29363-29368 JJ denotes human
T16898 29368-29370 , denotes ,
T16899 29370-29375 NN denotes chick
T16900 29376-29379 CC denotes and
T16901 29380-29389 NN denotes zebrafish
T16902 29389-29391 , denotes ,
T16903 29391-29403 RB denotes respectively
T16904 29404-29405 -LRB- denotes (
T16905 29410-29412 NN denotes 1D
T16906 29405-29409 NN denotes Fig.
T16907 29412-29413 -RRB- denotes )
T16908 29413-29414 . denotes .
T16909 29414-29557 sentence denotes This strongly suggests that the C-terminal region of mouse mr-s functions as a transcriptional repressive domain in photoreceptor development.
T16910 29415-29419 DT denotes This
T16911 29429-29437 VBZ denotes suggests
T16912 29420-29428 RB denotes strongly
T16913 29438-29442 IN denotes that
T16914 29479-29488 VBZ denotes functions
T16915 29443-29446 DT denotes the
T16916 29458-29464 NN denotes region
T16917 29447-29448 NN denotes C
T16918 29449-29457 JJ denotes terminal
T16919 29448-29449 HYPH denotes -
T16920 29465-29467 IN denotes of
T16921 29468-29473 NN denotes mouse
T16922 29477-29478 NN denotes s
T16923 29474-29476 NN denotes mr
T16924 29476-29477 HYPH denotes -
T16925 29489-29491 IN denotes as
T16926 29492-29493 DT denotes a
T16927 29521-29527 NN denotes domain
T16928 29494-29509 JJ denotes transcriptional
T16929 29510-29520 JJ denotes repressive
T16930 29528-29530 IN denotes in
T16931 29531-29544 NN denotes photoreceptor
T16932 29545-29556 NN denotes development
T16933 29556-29557 . denotes .
T16934 29557-29752 sentence denotes However, this region does not contain characteristic amino acid motifs and the mechanism through which the region achieves and/or maintains gene repression remains to be clarified in the future.
T16935 29558-29565 RB denotes However
T16936 29588-29595 VB denotes contain
T16937 29565-29567 , denotes ,
T16938 29567-29571 DT denotes this
T16939 29572-29578 NN denotes region
T16940 29579-29583 VBZ denotes does
T16968 29752-31435 sentence denotes Figure 7 mr-s fused to GAL4 DNA binding domain functions as a transcriptional repressor in HEK293T cells. (A) Schematic drawing of the constructs used for the luciferase assay. 5xGAL4-pGL3 reporter plasmid was co-transfected into HEK293T cells with effector plasmids expressing various deletion mutants fused to GAL4-DBD. (B) Various amounts of DBD, DBD-mrs, DBD-N or DBD-C plasmids were transfected with 0.1 μg of 5xGAL4-pGL3 reporter plasmid. The reporter activity in the presence of the pcDNA3 vector (pcDNA3) was designated as 1. Error bars represent standard error of mean. (C) DBD-W404A and DBD-G453A were co-transfected into HEK293T cells with 5xGAL4-pGL3 reporter plasmid. Fold repression was calculated as the fold decrease in luciferase activity compared with DBD-mrs. Error bars represent standard deviation. (D) Various amounts of DBD-tail or DBD-SAM were transfected with 5xGAL4-pGL3 reporter plasmid. Error bars represent standard error of mean. (E) pcDNA3 or DBD-mrs (5 μg) was co-transfected into Y79 retinoblastoma cells with 0.5 μg of 5xGAL4-pGL3 reporter plasmid. The reporter activity in the presence of pcDNA3 was designated as 1. Error bars represent standard deviation. Asterisk marks statistically significant difference (Student's t test: p < 0.03). (F) Alignment of the C-terminal regions for mouse, rat, human, chick and zebrafish mr-s proteins. Conserved amino acid residues are shown with a dark shadow. Taken together, our findings suggest that DBD-mrs functions as a transcriptional repressor and that the repression activity of mr-s is not due to a homophilic interaction through its SAM domain but to the C-terminal region (amino acids 463 to 542).
T16969 31187-31192 VBN denotes Taken
T16970 31216-31223 VBP denotes suggest
T16971 31193-31201 RB denotes together
T16972 31201-31203 , denotes ,
T16973 31203-31206 PRP$ denotes our
T16974 31207-31215 NNS denotes findings
T16975 31224-31228 IN denotes that
T16976 31237-31246 VBZ denotes functions
T16977 31229-31232 NN denotes DBD
T16978 31233-31236 NN denotes mrs
T16979 31232-31233 HYPH denotes -
T16980 31247-31249 IN denotes as
T16981 31250-31251 DT denotes a
T16982 31268-31277 NN denotes repressor
T16983 31252-31267 JJ denotes transcriptional
T16984 31278-31281 CC denotes and
T16985 31282-31286 IN denotes that
T16986 31319-31321 VBZ denotes is
T16987 31287-31290 DT denotes the
T16988 31302-31310 NN denotes activity
T16989 31291-31301 NN denotes repression
T16990 31311-31313 IN denotes of
T16991 31314-31316 NN denotes mr
T16992 31317-31318 NN denotes s
T16993 31316-31317 HYPH denotes -
T16994 31322-31325 RB denotes not
T16995 31326-31329 IN denotes due
T16996 31330-31332 IN denotes to
T16997 31333-31334 DT denotes a
T16998 31346-31357 NN denotes interaction
T16999 31335-31345 JJ denotes homophilic
T17000 31358-31365 IN denotes through
T17001 31366-31369 PRP$ denotes its
T17002 31374-31380 NN denotes domain
T17003 31370-31373 NN denotes SAM
T17004 31381-31384 CC denotes but
T17005 31385-31387 IN denotes to
T17006 31388-31391 DT denotes the
T17007 31403-31409 NN denotes region
T17008 31392-31393 NN denotes C
T17009 31394-31402 JJ denotes terminal
T17010 31393-31394 HYPH denotes -
T17011 31410-31411 -LRB- denotes (
T17012 31423-31426 CD denotes 463
T17013 31411-31416 NN denotes amino
T17014 31417-31422 NNS denotes acids
T17015 31427-31429 IN denotes to
T17016 31430-31433 CD denotes 542
T17017 31433-31434 -RRB- denotes )
T17018 31434-31435 . denotes .
T20693 31448-31450 IN denotes In
T20694 31473-31483 VBD denotes identified
T20695 31451-31454 DT denotes the
T20696 31463-31468 NN denotes study
T20697 31455-31462 JJ denotes present
T20698 31468-31470 , denotes ,
T20699 31470-31472 PRP denotes we
T20700 31484-31485 DT denotes a
T20701 31492-31496 NN denotes gene
T20702 31486-31491 JJ denotes novel
T20703 31496-31498 , denotes ,
T20704 31498-31500 NN denotes mr
T20705 31501-31502 NN denotes s
T20706 31500-31501 HYPH denotes -
T20707 31502-31504 , denotes ,
T20708 31504-31509 WDT denotes which
T20709 31527-31536 VBN denotes expressed
T20710 31510-31512 VBZ denotes is
T20711 31513-31526 RB denotes predominantly
T20712 31537-31539 IN denotes in
T20713 31540-31547 JJ denotes retinal
T20714 31548-31562 NNS denotes photoreceptors
T20715 31563-31566 CC denotes and
T20716 31567-31570 DT denotes the
T20717 31578-31583 NN denotes gland
T20718 31571-31577 JJ denotes pineal
T20719 31583-31584 . denotes .
T20720 31584-31651 sentence denotes The peak of mr-s expression in the developing retina is around P6.
T20721 31585-31588 DT denotes The
T20722 31589-31593 NN denotes peak
T20723 31638-31640 VBZ denotes is
T20724 31594-31596 IN denotes of
T20725 31597-31599 NN denotes mr
T20726 31600-31601 NN denotes s
T20727 31599-31600 HYPH denotes -
T20728 31602-31612 NN denotes expression
T20729 31613-31615 IN denotes in
T20730 31616-31619 DT denotes the
T20731 31631-31637 NN denotes retina
T20732 31620-31630 VBG denotes developing
T20733 31641-31647 IN denotes around
T20734 31648-31650 NN denotes P6
T20735 31650-31651 . denotes .
T20736 31651-31772 sentence denotes This expression pattern correlates with the rapid increase of Crx, rhodopsin and other photoreceptor genes around P6-P8.
T20737 31652-31656 DT denotes This
T20738 31668-31675 NN denotes pattern
T20739 31657-31667 NN denotes expression
T20740 31676-31686 VBZ denotes correlates
T20741 31687-31691 IN denotes with
T20742 31692-31695 DT denotes the
T20743 31702-31710 NN denotes increase
T20744 31696-31701 JJ denotes rapid
T20745 31711-31713 IN denotes of
T20746 31714-31717 NN denotes Crx
T20747 31717-31719 , denotes ,
T20748 31719-31728 NN denotes rhodopsin
T20749 31729-31732 CC denotes and
T20750 31733-31738 JJ denotes other
T20751 31753-31758 NNS denotes genes
T20752 31739-31752 NN denotes photoreceptor
T20753 31759-31765 IN denotes around
T20754 31766-31768 NN denotes P6
T20755 31768-31769 SYM denotes -
T20756 31769-31771 NN denotes P8
T20757 31771-31772 . denotes .
T20758 31772-31895 sentence denotes Around P6, the outer plexiform layer becomes visible and the outer layer of retina separates into two layers, ONL and INL.
T20759 31773-31779 IN denotes Around
T20760 31810-31817 VBZ denotes becomes
T20761 31780-31782 NN denotes P6
T20762 31782-31784 , denotes ,
T20763 31784-31787 DT denotes the
T20764 31804-31809 NN denotes layer
T20765 31788-31793 JJ denotes outer
T20766 31794-31803 NN denotes plexiform
T20767 31818-31825 JJ denotes visible
T20768 31826-31829 CC denotes and
T20769 31830-31833 DT denotes the
T20770 31840-31845 NN denotes layer
T20771 31834-31839 JJ denotes outer
T20772 31856-31865 VBZ denotes separates
T20773 31846-31848 IN denotes of
T20774 31849-31855 NN denotes retina
T20775 31866-31870 IN denotes into
T20776 31871-31874 CD denotes two
T20777 31875-31881 NNS denotes layers
T20778 31881-31883 , denotes ,
T20779 31883-31886 NN denotes ONL
T20780 31887-31890 CC denotes and
T20781 31891-31894 NN denotes INL
T20782 31894-31895 . denotes .
T20783 31895-31998 sentence denotes At the same time, photoreceptors begin to undergo terminal differentiation, forming the outer segment.
T20784 31896-31898 IN denotes At
T20785 31929-31934 VBP denotes begin
T20786 31899-31902 DT denotes the
T20787 31908-31912 NN denotes time
T20788 31903-31907 JJ denotes same
T20789 31912-31914 , denotes ,
T20790 31914-31928 NNS denotes photoreceptors
T20791 31935-31937 TO denotes to
T20792 31938-31945 VB denotes undergo
T20793 31946-31954 JJ denotes terminal
T20794 31955-31970 NN denotes differentiation
T20795 31970-31972 , denotes ,
T20796 31972-31979 VBG denotes forming
T20797 31980-31983 DT denotes the
T20798 31990-31997 NN denotes segment
T20799 31984-31989 JJ denotes outer
T20800 31997-31998 . denotes .
T20801 31998-32095 sentence denotes We therefore hypothesized that mr-s is a key molecule in the late development of photoreceptors.
T20802 31999-32001 PRP denotes We
T20803 32012-32024 VBD denotes hypothesized
T20804 32002-32011 RB denotes therefore
T20805 32025-32029 IN denotes that
T20806 32035-32037 VBZ denotes is
T20807 32030-32032 NN denotes mr
T20808 32033-32034 NN denotes s
T20809 32032-32033 HYPH denotes -
T20810 32038-32039 DT denotes a
T20811 32044-32052 NN denotes molecule
T20812 32040-32043 JJ denotes key
T20813 32053-32055 IN denotes in
T20814 32056-32059 DT denotes the
T20815 32065-32076 NN denotes development
T20816 32060-32064 JJ denotes late
T20817 32077-32079 IN denotes of
T20818 32080-32094 NNS denotes photoreceptors
T20819 32094-32095 . denotes .
T20820 32095-32244 sentence denotes We previously reported that Otx2 and Crx have a critical role in photoreceptor development and that Otx2 directly regulates Crx transcription [3,9].
T20821 32096-32098 PRP denotes We
T20822 32110-32118 VBD denotes reported
T20823 32099-32109 RB denotes previously
T20824 32119-32123 IN denotes that
T20825 32137-32141 VBP denotes have
T20826 32124-32128 NN denotes Otx2
T20827 32129-32132 CC denotes and
T20828 32133-32136 NN denotes Crx
T20829 32142-32143 DT denotes a
T20830 32153-32157 NN denotes role
T20831 32144-32152 JJ denotes critical
T20832 32158-32160 IN denotes in
T20833 32161-32174 NN denotes photoreceptor
T20834 32175-32186 NN denotes development
T20835 32187-32190 CC denotes and
T20836 32191-32195 IN denotes that
T20837 32210-32219 VBZ denotes regulates
T20838 32196-32200 NN denotes Otx2
T20839 32201-32209 RB denotes directly
T20840 32220-32223 NN denotes Crx
T20841 32224-32237 NN denotes transcription
T20842 32238-32239 -LRB- denotes [
T20843 32241-32242 CD denotes 9
T20844 32239-32240 CD denotes 3
T20845 32240-32241 , denotes ,
T20846 32242-32243 -RRB- denotes ]
T20847 32243-32244 . denotes .
T20848 32244-32360 sentence denotes In situ hybridization and RT-PCR showed significant reduction of mr-s signal in the Crx KO retina and pineal gland.
T20849 32245-32247 FW denotes In
T20850 32248-32252 FW denotes situ
T20851 32253-32266 NN denotes hybridization
T20852 32278-32284 VBD denotes showed
T20853 32267-32270 CC denotes and
T20854 32271-32273 NN denotes RT
T20855 32274-32277 NN denotes PCR
T20856 32273-32274 HYPH denotes -
T20857 32285-32296 JJ denotes significant
T20858 32297-32306 NN denotes reduction
T20859 32307-32309 IN denotes of
T20860 32310-32312 NN denotes mr
T20861 32313-32314 NN denotes s
T20862 32312-32313 HYPH denotes -
T20863 32315-32321 NN denotes signal
T20864 32322-32324 IN denotes in
T20865 32325-32328 DT denotes the
T20866 32333-32335 NN denotes KO
T20867 32329-32332 NN denotes Crx
T20868 32336-32342 NN denotes retina
T20869 32343-32346 CC denotes and
T20870 32347-32353 JJ denotes pineal
T20871 32354-32359 NN denotes gland
T20872 32359-32360 . denotes .
T20873 32360-32495 sentence denotes Furthermore, the luciferase assay demonstrated that Otx2 and Crx may directly upregulate the transcription of mr-s in mammalian cells.
T20874 32361-32372 RB denotes Furthermore
T20875 32395-32407 VBD denotes demonstrated
T20876 32372-32374 , denotes ,
T20877 32374-32377 DT denotes the
T20878 32389-32394 NN denotes assay
T20879 32378-32388 NN denotes luciferase
T20880 32408-32412 IN denotes that
T20881 32439-32449 VB denotes upregulate
T20882 32413-32417 NN denotes Otx2
T20883 32418-32421 CC denotes and
T20884 32422-32425 NN denotes Crx
T20885 32426-32429 MD denotes may
T20886 32430-32438 RB denotes directly
T20887 32450-32453 DT denotes the
T20888 32454-32467 NN denotes transcription
T20889 32468-32470 IN denotes of
T20890 32471-32473 NN denotes mr
T20891 32474-32475 NN denotes s
T20892 32473-32474 HYPH denotes -
T20893 32476-32478 IN denotes in
T20894 32479-32488 JJ denotes mammalian
T20895 32489-32494 NNS denotes cells
T20896 32494-32495 . denotes .
T20897 32495-32642 sentence denotes In retinal photoreceptor cells, the Otx2 transcripts are not highly expressed at P6-P9, while the Crx transcripts are strongly detected around P6.
T20898 32496-32498 IN denotes In
T20899 32549-32552 VBP denotes are
T20900 32499-32506 JJ denotes retinal
T20901 32521-32526 NNS denotes cells
T20902 32507-32520 NN denotes photoreceptor
T20903 32526-32528 , denotes ,
T20904 32528-32531 DT denotes the
T20905 32537-32548 NNS denotes transcripts
T20906 32532-32536 NN denotes Otx2
T20907 32553-32556 RB denotes not
T20908 32557-32563 RB denotes highly
T20909 32564-32573 JJ denotes expressed
T20910 32574-32576 IN denotes at
T20911 32577-32579 NN denotes P6
T20912 32579-32580 SYM denotes -
T20913 32580-32582 NN denotes P9
T20914 32582-32584 , denotes ,
T20915 32584-32589 IN denotes while
T20916 32623-32631 VBN denotes detected
T20917 32590-32593 DT denotes the
T20918 32598-32609 NNS denotes transcripts
T20919 32594-32597 NN denotes Crx
T20920 32610-32613 VBP denotes are
T20921 32614-32622 RB denotes strongly
T20922 32632-32638 IN denotes around
T20923 32639-32641 NN denotes P6
T20924 32641-32642 . denotes .
T20925 32642-32736 sentence denotes Therefore, our results strongly suggest that mr-s transcription is directly regulated by Crx.
T20926 32643-32652 RB denotes Therefore
T20927 32675-32682 VBP denotes suggest
T20928 32652-32654 , denotes ,
T20929 32654-32657 PRP$ denotes our
T20930 32658-32665 NNS denotes results
T20931 32666-32674 RB denotes strongly
T20932 32683-32687 IN denotes that
T20933 32719-32728 VBN denotes regulated
T20934 32688-32690 NN denotes mr
T20935 32691-32692 NN denotes s
T20936 32690-32691 HYPH denotes -
T20937 32693-32706 NN denotes transcription
T20938 32707-32709 VBZ denotes is
T20939 32710-32718 RB denotes directly
T20940 32729-32731 IN denotes by
T20941 32732-32735 NN denotes Crx
T20942 32735-32736 . denotes .
T20943 32736-32918 sentence denotes In the present study, Nrl, a photoreceptor-specific transcription factor that is highly expressed in photoreceptors at the postnatal stage, did not affect the transcription of mr-s.
T20944 32737-32739 IN denotes In
T20945 32885-32891 VB denotes affect
T20946 32740-32743 DT denotes the
T20947 32752-32757 NN denotes study
T20948 32744-32751 JJ denotes present
T20949 32757-32759 , denotes ,
T20950 32759-32762 NN denotes Nrl
T20951 32762-32764 , denotes ,
T20952 32764-32765 DT denotes a
T20953 32803-32809 NN denotes factor
T20954 32766-32779 NN denotes photoreceptor
T20955 32780-32788 JJ denotes specific
T20956 32779-32780 HYPH denotes -
T20957 32789-32802 NN denotes transcription
T20958 32810-32814 WDT denotes that
T20959 32825-32834 VBN denotes expressed
T20960 32815-32817 VBZ denotes is
T20961 32818-32824 RB denotes highly
T20962 32835-32837 IN denotes in
T20963 32838-32852 NNS denotes photoreceptors
T20964 32853-32855 IN denotes at
T20965 32856-32859 DT denotes the
T20966 32870-32875 NN denotes stage
T20967 32860-32869 JJ denotes postnatal
T20968 32875-32877 , denotes ,
T20969 32877-32880 VBD denotes did
T20970 32881-32884 RB denotes not
T20971 32892-32895 DT denotes the
T20972 32896-32909 NN denotes transcription
T20973 32910-32912 IN denotes of
T20974 32913-32915 NN denotes mr
T20975 32916-32917 NN denotes s
T20976 32915-32916 HYPH denotes -
T20977 32917-32918 . denotes .
T20978 32918-33026 sentence denotes This finding is actually consistent with the analysis of the Nrl KO mouse which was recently reported [40].
T20979 32919-32923 DT denotes This
T20980 32924-32931 NN denotes finding
T20981 32932-32934 VBZ denotes is
T20982 32935-32943 RB denotes actually
T20983 32944-32954 JJ denotes consistent
T20984 32955-32959 IN denotes with
T20985 32960-32963 DT denotes the
T20986 32964-32972 NN denotes analysis
T20987 32973-32975 IN denotes of
T20988 32976-32979 DT denotes the
T20989 32987-32992 NN denotes mouse
T20990 32980-32983 NN denotes Nrl
T20991 32984-32986 NN denotes KO
T20992 32993-32998 WDT denotes which
T20993 33012-33020 VBN denotes reported
T20994 32999-33002 VBD denotes was
T20995 33003-33011 RB denotes recently
T20996 33021-33022 -LRB- denotes [
T20997 33022-33024 CD denotes 40
T20998 33024-33025 -RRB- denotes ]
T20999 33025-33026 . denotes .
T21000 33026-33206 sentence denotes The expression profiles of wild-type and Nrl KO retinas at P2, P10 and 2 months were analyzed and mr-s was not included in 161 differentially expressed genes in the Nrl KO retina.
T21001 33027-33030 DT denotes The
T21002 33042-33050 NNS denotes profiles
T21003 33031-33041 NN denotes expression
T21004 33112-33120 VBN denotes analyzed
T21005 33051-33053 IN denotes of
T21006 33054-33058 JJ denotes wild
T21007 33059-33063 NN denotes type
T21008 33058-33059 HYPH denotes -
T21009 33075-33082 NNS denotes retinas
T21010 33064-33067 CC denotes and
T21011 33068-33071 NN denotes Nrl
T21012 33072-33074 NN denotes KO
T21013 33083-33085 IN denotes at
T21014 33086-33088 NN denotes P2
T21015 33088-33090 , denotes ,
T21016 33090-33093 NN denotes P10
T21017 33094-33097 CC denotes and
T21018 33098-33099 CD denotes 2
T21019 33100-33106 NNS denotes months
T21020 33107-33111 VBD denotes were
T21021 33121-33124 CC denotes and
T21022 33125-33127 NN denotes mr
T21023 33128-33129 NN denotes s
T21024 33127-33128 HYPH denotes -
T21025 33138-33146 VBN denotes included
T21026 33130-33133 VBD denotes was
T21027 33134-33137 RB denotes not
T21028 33147-33149 IN denotes in
T21029 33150-33153 CD denotes 161
T21030 33179-33184 NNS denotes genes
T21031 33154-33168 RB denotes differentially
T21032 33169-33178 VBN denotes expressed
T21033 33185-33187 IN denotes in
T21034 33188-33191 DT denotes the
T21035 33199-33205 NN denotes retina
T21036 33192-33195 NN denotes Nrl
T21037 33196-33198 NN denotes KO
T21038 33205-33206 . denotes .
T21039 33206-33294 sentence denotes Previous reports suggested that the SAM domain is a protein-protein interaction module.
T21040 33207-33215 JJ denotes Previous
T21041 33216-33223 NNS denotes reports
T21042 33224-33233 VBD denotes suggested
T21043 33234-33238 IN denotes that
T21044 33254-33256 VBZ denotes is
T21045 33239-33242 DT denotes the
T21046 33247-33253 NN denotes domain
T21047 33243-33246 NN denotes SAM
T21048 33257-33258 DT denotes a
T21049 33287-33293 NN denotes module
T21050 33259-33266 NN denotes protein
T21051 33267-33274 NN denotes protein
T21052 33266-33267 HYPH denotes -
T21053 33275-33286 NN denotes interaction
T21054 33293-33294 . denotes .
T21055 33294-33507 sentence denotes The SAM domain of the mr-s protein is closely related to that of ph and TEL, whose SAM domains can form a helical, head-to-tail polymeric structure and mediate the formation of a higher order chromatin structure.
T21056 33295-33298 DT denotes The
T21057 33303-33309 NN denotes domain
T21058 33299-33302 NN denotes SAM
T21059 33341-33348 VBN denotes related
T21060 33310-33312 IN denotes of
T21061 33313-33316 DT denotes the
T21062 33322-33329 NN denotes protein
T21063 33317-33319 NN denotes mr
T21064 33320-33321 NN denotes s
T21065 33319-33320 HYPH denotes -
T21066 33330-33332 VBZ denotes is
T21067 33333-33340 RB denotes closely
T21068 33349-33351 IN denotes to
T21069 33352-33356 DT denotes that
T21070 33357-33359 IN denotes of
T21071 33360-33362 NN denotes ph
T21072 33363-33366 CC denotes and
T21073 33367-33370 NN denotes TEL
T21074 33370-33372 , denotes ,
T21075 33372-33377 WP$ denotes whose
T21076 33382-33389 NNS denotes domains
T21077 33378-33381 NN denotes SAM
T21078 33394-33398 VB denotes form
T21079 33390-33393 MD denotes can
T21080 33399-33400 DT denotes a
T21081 33433-33442 NN denotes structure
T21082 33401-33408 JJ denotes helical
T21083 33408-33410 , denotes ,
T21084 33410-33414 NN denotes head
T21085 33414-33415 HYPH denotes -
T21086 33415-33417 IN denotes to
T21087 33417-33418 HYPH denotes -
T21088 33418-33422 NN denotes tail
T21089 33423-33432 JJ denotes polymeric
T21090 33443-33446 CC denotes and
T21091 33447-33454 VB denotes mediate
T21092 33455-33458 DT denotes the
T21093 33459-33468 NN denotes formation
T21094 33469-33471 IN denotes of
T21095 33472-33473 DT denotes a
T21096 33497-33506 NN denotes structure
T21097 33474-33480 JJR denotes higher
T21098 33481-33486 NN denotes order
T21099 33487-33496 NN denotes chromatin
T21100 33506-33507 . denotes .
T21101 33507-33633 sentence denotes To characterize the biochemical function of mr-s, we performed yeast two-hybrid screening using full-length mr-s as the bait.
T21102 33508-33510 TO denotes To
T21103 33511-33523 VB denotes characterize
T21104 33561-33570 VBD denotes performed
T21105 33524-33527 DT denotes the
T21106 33540-33548 NN denotes function
T21107 33528-33539 JJ denotes biochemical
T21108 33549-33551 IN denotes of
T21109 33552-33554 NN denotes mr
T21110 33555-33556 NN denotes s
T21111 33554-33555 HYPH denotes -
T21112 33556-33558 , denotes ,
T21113 33558-33560 PRP denotes we
T21114 33571-33576 NN denotes yeast
T21115 33588-33597 NN denotes screening
T21116 33577-33580 CD denotes two
T21117 33581-33587 NN denotes hybrid
T21118 33580-33581 HYPH denotes -
T21119 33598-33603 VBG denotes using
T21120 33604-33608 JJ denotes full
T21121 33609-33615 NN denotes length
T21122 33608-33609 HYPH denotes -
T21123 33619-33620 NN denotes s
T21124 33616-33618 NN denotes mr
T21125 33618-33619 HYPH denotes -
T21126 33621-33623 IN denotes as
T21127 33624-33627 DT denotes the
T21128 33628-33632 NN denotes bait
T21129 33632-33633 . denotes .
T21130 33633-33763 sentence denotes As a result, the most frequent positive clones (5/28) in the screening were the cDNA fragments containing the SAM domain of mr-s.
T21131 33634-33636 IN denotes As
T21132 33705-33709 VBD denotes were
T21133 33637-33638 DT denotes a
T21134 33639-33645 NN denotes result
T21135 33645-33647 , denotes ,
T21136 33647-33650 DT denotes the
T21137 33674-33680 NNS denotes clones
T21138 33651-33655 RBS denotes most
T21139 33656-33664 JJ denotes frequent
T21140 33665-33673 JJ denotes positive
T21141 33681-33682 -LRB- denotes (
T21142 33682-33683 CD denotes 5
T21143 33683-33684 SYM denotes /
T21144 33684-33686 CD denotes 28
T21145 33686-33687 -RRB- denotes )
T21146 33688-33690 IN denotes in
T21147 33691-33694 DT denotes the
T21148 33695-33704 NN denotes screening
T21149 33710-33713 DT denotes the
T21150 33719-33728 NNS denotes fragments
T21151 33714-33718 NN denotes cDNA
T21152 33729-33739 VBG denotes containing
T21153 33740-33743 DT denotes the
T21154 33748-33754 NN denotes domain
T21155 33744-33747 NN denotes SAM
T21156 33755-33757 IN denotes of
T21157 33758-33760 NN denotes mr
T21158 33761-33762 NN denotes s
T21159 33760-33761 HYPH denotes -
T21160 33762-33763 . denotes .
T21161 33763-33836 sentence denotes This strongly suggests that mr-s self-associates through its SAM domain.
T21162 33764-33768 DT denotes This
T21163 33778-33786 VBZ denotes suggests
T21164 33769-33777 RB denotes strongly
T21165 33787-33791 IN denotes that
T21166 33802-33812 VBZ denotes associates
T21167 33792-33794 NN denotes mr
T21168 33795-33796 NN denotes s
T21169 33794-33795 HYPH denotes -
T21170 33797-33801 NN denotes self
T21171 33801-33802 HYPH denotes -
T21172 33813-33820 IN denotes through
T21173 33821-33824 PRP$ denotes its
T21174 33829-33835 NN denotes domain
T21175 33825-33828 NN denotes SAM
T21176 33835-33836 . denotes .
T21177 33836-34022 sentence denotes An immunoprecipitation assay, using two site-directed mutants of the SAM domain of mr-s, demonstrated that the mr-s protein can self-associate through its SAM domain in mammalian cells.
T21178 33837-33839 DT denotes An
T21179 33860-33865 NN denotes assay
T21180 33840-33859 NN denotes immunoprecipitation
T21181 33926-33938 VBD denotes demonstrated
T21182 33865-33867 , denotes ,
T21183 33867-33872 VBG denotes using
T21184 33873-33876 CD denotes two
T21185 33891-33898 NNS denotes mutants
T21186 33877-33881 NN denotes site
T21187 33882-33890 VBN denotes directed
T21188 33881-33882 HYPH denotes -
T21189 33899-33901 IN denotes of
T21190 33902-33905 DT denotes the
T21191 33910-33916 NN denotes domain
T21192 33906-33909 NN denotes SAM
T21193 33917-33919 IN denotes of
T21194 33920-33922 NN denotes mr
T21195 33923-33924 NN denotes s
T21196 33922-33923 HYPH denotes -
T21197 33924-33926 , denotes ,
T21198 33939-33943 IN denotes that
T21199 33970-33979 VB denotes associate
T21200 33944-33947 DT denotes the
T21201 33953-33960 NN denotes protein
T21202 33948-33950 NN denotes mr
T21203 33951-33952 NN denotes s
T21204 33950-33951 HYPH denotes -
T21205 33961-33964 MD denotes can
T21206 33965-33969 NN denotes self
T21207 33969-33970 HYPH denotes -
T21208 33980-33987 IN denotes through
T21209 33988-33991 PRP$ denotes its
T21210 33996-34002 NN denotes domain
T21211 33992-33995 NN denotes SAM
T21212 34003-34005 IN denotes in
T21213 34006-34015 JJ denotes mammalian
T21214 34016-34021 NNS denotes cells
T21215 34021-34022 . denotes .
T21216 34022-34387 sentence denotes While we did not address the question whether the SAM domain of mr-s forms a polymeric structure in the present study, the phylogenetic analysis of SAM domain of mr-s and other SAM domain-containing molecules suggests that mr-s can form head-to-tail polymer and mediate gene silencing by spreading repressive complexes along the chromatin similar to ph and/or TEL.
T21217 34023-34028 IN denotes While
T21218 34040-34047 VB denotes address
T21219 34029-34031 PRP denotes we
T21220 34032-34035 VBD denotes did
T21221 34036-34039 RB denotes not
T21222 34232-34240 VBZ denotes suggests
T21223 34048-34051 DT denotes the
T21224 34052-34060 NN denotes question
T21225 34061-34068 IN denotes whether
T21226 34092-34097 VBZ denotes forms
T21227 34069-34072 DT denotes the
T21228 34077-34083 NN denotes domain
T21229 34073-34076 NN denotes SAM
T21230 34084-34086 IN denotes of
T21231 34087-34089 NN denotes mr
T21232 34090-34091 NN denotes s
T21233 34089-34090 HYPH denotes -
T21234 34098-34099 DT denotes a
T21235 34110-34119 NN denotes structure
T21236 34100-34109 JJ denotes polymeric
T21237 34120-34122 IN denotes in
T21238 34123-34126 DT denotes the
T21239 34135-34140 NN denotes study
T21240 34127-34134 JJ denotes present
T21241 34140-34142 , denotes ,
T21242 34142-34145 DT denotes the
T21243 34159-34167 NN denotes analysis
T21244 34146-34158 JJ denotes phylogenetic
T21245 34168-34170 IN denotes of
T21246 34171-34174 NN denotes SAM
T21247 34175-34181 NN denotes domain
T21248 34182-34184 IN denotes of
T21249 34185-34187 NN denotes mr
T21250 34188-34189 NN denotes s
T21251 34187-34188 HYPH denotes -
T21252 34190-34193 CC denotes and
T21253 34194-34199 JJ denotes other
T21254 34222-34231 NNS denotes molecules
T21255 34200-34203 NN denotes SAM
T21256 34204-34210 NN denotes domain
T21257 34211-34221 VBG denotes containing
T21258 34210-34211 HYPH denotes -
T21259 34241-34245 IN denotes that
T21260 34255-34259 VB denotes form
T21261 34246-34248 NN denotes mr
T21262 34249-34250 NN denotes s
T21263 34248-34249 HYPH denotes -
T21264 34251-34254 MD denotes can
T21265 34260-34264 NN denotes head
T21266 34273-34280 NN denotes polymer
T21267 34264-34265 HYPH denotes -
T21268 34265-34267 IN denotes to
T21269 34267-34268 HYPH denotes -
T21270 34268-34272 NN denotes tail
T21271 34281-34284 CC denotes and
T21272 34285-34292 VBP denotes mediate
T21273 34293-34297 NN denotes gene
T21274 34298-34307 NN denotes silencing
T21275 34308-34310 IN denotes by
T21276 34311-34320 VBG denotes spreading
T21277 34321-34331 JJ denotes repressive
T21278 34332-34341 NNS denotes complexes
T21279 34342-34347 IN denotes along
T21280 34348-34351 DT denotes the
T21281 34352-34361 NN denotes chromatin
T21282 34362-34369 JJ denotes similar
T21283 34370-34372 IN denotes to
T21284 34373-34375 NN denotes ph
T21285 34376-34379 CC denotes and
T21286 34379-34380 HYPH denotes /
T21287 34380-34382 CC denotes or
T21288 34383-34386 NN denotes TEL
T21289 34386-34387 . denotes .
T21290 34387-34600 sentence denotes Although our results in the immunoprecipitation assay demonstrated that the N-terminal constructs lacking a SAM domain still interact with each other, these results do not fit into the head-to-tail polymer model.
T21291 34388-34396 IN denotes Although
T21292 34442-34454 VBD denotes demonstrated
T21293 34397-34400 PRP$ denotes our
T21294 34401-34408 NNS denotes results
T21295 34409-34411 IN denotes in
T21296 34412-34415 DT denotes the
T21297 34436-34441 NN denotes assay
T21298 34416-34435 NN denotes immunoprecipitation
T21299 34560-34563 VB denotes fit
T21300 34455-34459 IN denotes that
T21301 34513-34521 VBP denotes interact
T21302 34460-34463 DT denotes the
T21303 34475-34485 NNS denotes constructs
T21304 34464-34465 NN denotes N
T21305 34466-34474 JJ denotes terminal
T21306 34465-34466 HYPH denotes -
T21307 34486-34493 VBG denotes lacking
T21308 34494-34495 DT denotes a
T21309 34500-34506 NN denotes domain
T21310 34496-34499 NN denotes SAM
T21311 34507-34512 RB denotes still
T21312 34522-34526 IN denotes with
T21313 34527-34531 DT denotes each
T21314 34532-34537 JJ denotes other
T21315 34537-34539 , denotes ,
T21316 34539-34544 DT denotes these
T21317 34545-34552 NNS denotes results
T21318 34553-34555 VBP denotes do
T21319 34556-34559 RB denotes not
T21320 34564-34568 IN denotes into
T21321 34569-34572 DT denotes the
T21322 34594-34599 NN denotes model
T21323 34573-34577 NN denotes head
T21324 34577-34578 HYPH denotes -
T21325 34578-34580 IN denotes to
T21326 34580-34581 HYPH denotes -
T21327 34581-34585 NN denotes tail
T21328 34586-34593 NN denotes polymer
T21329 34599-34600 . denotes .
T21330 34600-34738 sentence denotes We cannot exclude the possibility that the resulting protein-protein interaction of mr-s is an artifact of the overexpression conditions.
T21331 34601-34603 PRP denotes We
T21332 34611-34618 VB denotes exclude
T21333 34604-34607 MD denotes can
T21334 34607-34610 RB denotes not
T21335 34619-34622 DT denotes the
T21336 34623-34634 NN denotes possibility
T21337 34635-34639 IN denotes that
T21338 34690-34692 VBZ denotes is
T21339 34640-34643 DT denotes the
T21340 34670-34681 NN denotes interaction
T21341 34644-34653 VBG denotes resulting
T21342 34654-34661 NN denotes protein
T21343 34662-34669 NN denotes protein
T21344 34661-34662 HYPH denotes -
T21345 34682-34684 IN denotes of
T21346 34685-34687 NN denotes mr
T21347 34688-34689 NN denotes s
T21348 34687-34688 HYPH denotes -
T21349 34693-34695 DT denotes an
T21350 34696-34704 NN denotes artifact
T21351 34705-34707 IN denotes of
T21352 34708-34711 DT denotes the
T21353 34727-34737 NNS denotes conditions
T21354 34712-34726 NN denotes overexpression
T21355 34737-34738 . denotes .
T21356 34738-34811 sentence denotes The issue of whether or not mr-s forms a polymer awaits future analysis.
T21357 34739-34742 DT denotes The
T21358 34743-34748 NN denotes issue
T21359 34788-34794 VBZ denotes awaits
T21360 34749-34751 IN denotes of
T21361 34752-34759 IN denotes whether
T21362 34772-34777 VBZ denotes forms
T21363 34760-34762 CC denotes or
T21364 34763-34766 RB denotes not
T21365 34767-34769 NN denotes mr
T21366 34770-34771 NN denotes s
T21367 34769-34770 HYPH denotes -
T21368 34778-34779 DT denotes a
T21369 34780-34787 NN denotes polymer
T21370 34795-34801 JJ denotes future
T21371 34802-34810 NN denotes analysis
T21372 34810-34811 . denotes .
T21373 34811-34973 sentence denotes A previous report indicated that TEL contains a sequence-specific DNA binding domain, namely the ETS domain, and binds to specific sites via its ETS domain [41].
T21374 34812-34813 DT denotes A
T21375 34823-34829 NN denotes report
T21376 34814-34822 JJ denotes previous
T21377 34830-34839 VBD denotes indicated
T21378 34840-34844 IN denotes that
T21379 34849-34857 VBZ denotes contains
T21380 34845-34848 NN denotes TEL
T21381 34858-34859 DT denotes a
T21382 34890-34896 NN denotes domain
T21383 34860-34868 NN denotes sequence
T21384 34869-34877 JJ denotes specific
T21385 34868-34869 HYPH denotes -
T21386 34878-34881 NN denotes DNA
T21387 34882-34889 VBG denotes binding
T21388 34896-34898 , denotes ,
T21389 34913-34919 NN denotes domain
T21390 34898-34904 RB denotes namely
T21391 34905-34908 DT denotes the
T21392 34909-34912 NN denotes ETS
T21393 34919-34921 , denotes ,
T21394 34921-34924 CC denotes and
T21395 34925-34930 VBZ denotes binds
T21396 34931-34933 IN denotes to
T21397 34934-34942 JJ denotes specific
T21398 34943-34948 NNS denotes sites
T21399 34949-34952 IN denotes via
T21400 34953-34956 PRP$ denotes its
T21401 34961-34967 NN denotes domain
T21402 34957-34960 NN denotes ETS
T21403 34968-34969 -LRB- denotes [
T21404 34969-34971 CD denotes 41
T21405 34971-34972 -RRB- denotes ]
T21406 34972-34973 . denotes .
T21407 34973-35069 sentence denotes TEL could serve to nucleate a polymer, which would spread by oligomerization of the SAM domain.
T21408 34974-34977 NN denotes TEL
T21409 34984-34989 VB denotes serve
T21410 34978-34983 MD denotes could
T21411 34990-34992 TO denotes to
T21412 34993-35001 VB denotes nucleate
T21413 35002-35003 DT denotes a
T21414 35004-35011 NN denotes polymer
T21415 35011-35013 , denotes ,
T21416 35013-35018 WDT denotes which
T21417 35025-35031 VB denotes spread
T21418 35019-35024 MD denotes would
T21419 35032-35034 IN denotes by
T21420 35035-35050 NN denotes oligomerization
T21421 35051-35053 IN denotes of
T21422 35054-35057 DT denotes the
T21423 35062-35068 NN denotes domain
T21424 35058-35061 NN denotes SAM
T21425 35068-35069 . denotes .
T21426 35069-35162 sentence denotes In contrast to TEL, ph does not contain an obvious sequence-specific DNA binding motif (16).
T21427 35070-35072 IN denotes In
T21428 35102-35109 VB denotes contain
T21429 35073-35081 NN denotes contrast
T21430 35082-35084 IN denotes to
T21431 35085-35088 NN denotes TEL
T21432 35088-35090 , denotes ,
T21433 35090-35092 NN denotes ph
T21434 35093-35097 VBZ denotes does
T21435 35098-35101 RB denotes not
T21436 35110-35112 DT denotes an
T21437 35151-35156 NN denotes motif
T21438 35113-35120 JJ denotes obvious
T21439 35121-35129 NN denotes sequence
T21440 35130-35138 JJ denotes specific
T21441 35129-35130 HYPH denotes -
T21442 35139-35142 NN denotes DNA
T21443 35143-35150 VBG denotes binding
T21444 35157-35158 -LRB- denotes (
T21445 35158-35160 CD denotes 16
T21446 35160-35161 -RRB- denotes )
T21447 35161-35162 . denotes .
T21448 35162-35307 sentence denotes Therefore, its initial binding to the template may require protein-protein interactions with other sequence-specific transcriptional repressors.
T21449 35163-35172 RB denotes Therefore
T21450 35214-35221 VB denotes require
T21451 35172-35174 , denotes ,
T21452 35174-35177 PRP$ denotes its
T21453 35186-35193 NN denotes binding
T21454 35178-35185 JJ denotes initial
T21455 35194-35196 IN denotes to
T21456 35197-35200 DT denotes the
T21457 35201-35209 NN denotes template
T21458 35210-35213 MD denotes may
T21459 35222-35229 NN denotes protein
T21460 35230-35237 NN denotes protein
T21461 35229-35230 HYPH denotes -
T21462 35238-35250 NNS denotes interactions
T21463 35251-35255 IN denotes with
T21464 35256-35261 JJ denotes other
T21465 35296-35306 NNS denotes repressors
T21466 35262-35270 NN denotes sequence
T21467 35271-35279 JJ denotes specific
T21468 35270-35271 HYPH denotes -
T21469 35280-35295 JJ denotes transcriptional
T21470 35306-35307 . denotes .
T21471 35307-35506 sentence denotes The segmentation gene-encoding transcriptional repressors such as Hunchback have a role in recruiting SAM domain-containing PcG proteins, which can spread along the template via polymerization [42].
T21472 35308-35311 DT denotes The
T21473 35355-35365 NNS denotes repressors
T21474 35312-35324 NN denotes segmentation
T21475 35325-35329 NN denotes gene
T21476 35330-35338 VBG denotes encoding
T21477 35329-35330 HYPH denotes -
T21478 35339-35354 JJ denotes transcriptional
T21479 35384-35388 VBP denotes have
T21480 35366-35370 JJ denotes such
T21481 35371-35373 IN denotes as
T21482 35374-35383 NN denotes Hunchback
T21483 35389-35390 DT denotes a
T21484 35391-35395 NN denotes role
T21485 35396-35398 IN denotes in
T21486 35399-35409 VBG denotes recruiting
T21487 35410-35413 NN denotes SAM
T21488 35436-35444 NN denotes proteins
T21489 35414-35420 NN denotes domain
T21490 35421-35431 VBG denotes containing
T21491 35420-35421 HYPH denotes -
T21492 35432-35435 NN denotes PcG
T21493 35444-35446 , denotes ,
T21494 35446-35451 WDT denotes which
T21495 35456-35462 VB denotes spread
T21496 35452-35455 MD denotes can
T21497 35463-35468 IN denotes along
T21498 35469-35472 DT denotes the
T21499 35473-35481 NN denotes template
T21500 35482-35485 IN denotes via
T21501 35486-35500 NN denotes polymerization
T21502 35501-35502 -LRB- denotes [
T21503 35502-35504 CD denotes 42
T21504 35504-35505 -RRB- denotes ]
T21505 35505-35506 . denotes .
T21506 35506-35658 sentence denotes Since mr-s does not contain obvious DNA binding motifs, we suppose that there is a sequence-specific transcription factor(s) which interacts with mr-s.
T21507 35507-35512 IN denotes Since
T21508 35527-35534 VB denotes contain
T21509 35513-35515 NN denotes mr
T21510 35516-35517 NN denotes s
T21511 35515-35516 HYPH denotes -
T21512 35518-35522 VBZ denotes does
T21513 35523-35526 RB denotes not
T21514 35566-35573 VBP denotes suppose
T21515 35535-35542 JJ denotes obvious
T21516 35555-35561 NNS denotes motifs
T21517 35543-35546 NN denotes DNA
T21518 35547-35554 VBG denotes binding
T21519 35561-35563 , denotes ,
T21520 35563-35565 PRP denotes we
T21521 35574-35578 IN denotes that
T21522 35585-35587 VBZ denotes is
T21523 35579-35584 EX denotes there
T21524 35588-35589 DT denotes a
T21525 35622-35628 NN denotes factor
T21526 35590-35598 NN denotes sequence
T21527 35599-35607 JJ denotes specific
T21528 35598-35599 HYPH denotes -
T21529 35608-35621 NN denotes transcription
T21530 35628-35629 -LRB- denotes (
T21531 35629-35630 AFX denotes s
T21532 35630-35631 -RRB- denotes )
T21533 35632-35637 WDT denotes which
T21534 35638-35647 VBZ denotes interacts
T21535 35648-35652 IN denotes with
T21536 35653-35655 NN denotes mr
T21537 35656-35657 NN denotes s
T21538 35655-35656 HYPH denotes -
T21539 35657-35658 . denotes .
T21540 35658-35752 sentence denotes However, we did not find any transcription factors in the present yeast two-hybrid screening.
T21541 35659-35666 RB denotes However
T21542 35679-35683 VB denotes find
T21543 35666-35668 , denotes ,
T21544 35668-35670 PRP denotes we
T21545 35671-35674 VBD denotes did
T21546 35675-35678 RB denotes not
T21547 35684-35687 DT denotes any
T21548 35702-35709 NNS denotes factors
T21549 35688-35701 NN denotes transcription
T21550 35710-35712 IN denotes in
T21551 35713-35716 DT denotes the
T21552 35742-35751 NN denotes screening
T21553 35717-35724 JJ denotes present
T21554 35725-35730 NN denotes yeast
T21555 35731-35734 CD denotes two
T21556 35735-35741 NN denotes hybrid
T21557 35734-35735 HYPH denotes -
T21558 35751-35752 . denotes .
T21559 35752-35877 sentence denotes We also found that full-length mr-s fused to the GAL4 DNA binding domain (DBD-mrs) functions as a transcriptional repressor.
T21560 35753-35755 PRP denotes We
T21561 35761-35766 VBD denotes found
T21562 35756-35760 RB denotes also
T21563 35767-35771 IN denotes that
T21564 35836-35845 VBZ denotes functions
T21565 35772-35776 JJ denotes full
T21566 35777-35783 NN denotes length
T21567 35776-35777 HYPH denotes -
T21568 35787-35788 NN denotes s
T21569 35784-35786 NN denotes mr
T21570 35786-35787 HYPH denotes -
T21571 35789-35794 VBN denotes fused
T21572 35795-35797 IN denotes to
T21573 35798-35801 DT denotes the
T21574 35819-35825 NN denotes domain
T21575 35802-35806 NN denotes GAL4
T21576 35807-35810 NN denotes DNA
T21577 35811-35818 VBG denotes binding
T21578 35826-35827 -LRB- denotes (
T21579 35831-35834 NN denotes mrs
T21580 35827-35830 NN denotes DBD
T21581 35830-35831 HYPH denotes -
T21582 35834-35835 -RRB- denotes )
T21583 35846-35848 IN denotes as
T21584 35849-35850 DT denotes a
T21585 35867-35876 NN denotes repressor
T21586 35851-35866 JJ denotes transcriptional
T21587 35876-35877 . denotes .
T21588 35877-35998 sentence denotes This may support the idea that mr-s is involved in repressive complexes similar to other SAM domain-containing proteins.
T21589 35878-35882 DT denotes This
T21590 35887-35894 VB denotes support
T21591 35883-35886 MD denotes may
T21592 35895-35898 DT denotes the
T21593 35899-35903 NN denotes idea
T21594 35904-35908 IN denotes that
T21595 35917-35925 VBN denotes involved
T21596 35909-35911 NN denotes mr
T21597 35912-35913 NN denotes s
T21598 35911-35912 HYPH denotes -
T21599 35914-35916 VBZ denotes is
T21600 35926-35928 IN denotes in
T21601 35929-35939 JJ denotes repressive
T21602 35940-35949 NNS denotes complexes
T21603 35950-35957 JJ denotes similar
T21604 35958-35960 IN denotes to
T21605 35961-35966 JJ denotes other
T21606 35989-35997 NN denotes proteins
T21607 35967-35970 NN denotes SAM
T21608 35971-35977 NN denotes domain
T21609 35978-35988 VBG denotes containing
T21610 35977-35978 HYPH denotes -
T21611 35997-35998 . denotes .
T21612 35998-36146 sentence denotes Our results, however, showed that the self-association of mr-s through its SAM domain is not essential for the transcriptional repressive activity.
T21613 35999-36002 PRP$ denotes Our
T21614 36003-36010 NNS denotes results
T21615 36021-36027 VBD denotes showed
T21616 36010-36012 , denotes ,
T21617 36012-36019 RB denotes however
T21618 36019-36021 , denotes ,
T21619 36028-36032 IN denotes that
T21620 36085-36087 VBZ denotes is
T21621 36033-36036 DT denotes the
T21622 36042-36053 NN denotes association
T21623 36037-36041 NN denotes self
T21624 36041-36042 HYPH denotes -
T21625 36054-36056 IN denotes of
T21626 36057-36059 NN denotes mr
T21627 36060-36061 NN denotes s
T21628 36059-36060 HYPH denotes -
T21629 36062-36069 IN denotes through
T21630 36070-36073 PRP$ denotes its
T21631 36078-36084 NN denotes domain
T21632 36074-36077 NN denotes SAM
T21633 36088-36091 RB denotes not
T21634 36092-36101 JJ denotes essential
T21635 36102-36105 IN denotes for
T21636 36106-36109 DT denotes the
T21637 36137-36145 NN denotes activity
T21638 36110-36125 JJ denotes transcriptional
T21639 36126-36136 JJ denotes repressive
T21640 36145-36146 . denotes .
T21641 36146-36276 sentence denotes Polymerization of the SAM domain has been previously reported to be essential for the repressive functions of ph and TEL [43,44].
T21642 36147-36161 NN denotes Polymerization
T21643 36200-36208 VBN denotes reported
T21644 36162-36164 IN denotes of
T21645 36165-36168 DT denotes the
T21646 36173-36179 NN denotes domain
T21647 36169-36172 NN denotes SAM
T21648 36180-36183 VBZ denotes has
T21649 36184-36188 VBN denotes been
T21650 36189-36199 RB denotes previously
T21651 36209-36211 TO denotes to
T21652 36212-36214 VB denotes be
T21653 36215-36224 JJ denotes essential
T21654 36225-36228 IN denotes for
T21655 36229-36232 DT denotes the
T21656 36244-36253 NNS denotes functions
T21657 36233-36243 JJ denotes repressive
T21658 36254-36256 IN denotes of
T21659 36257-36259 NN denotes ph
T21660 36260-36263 CC denotes and
T21661 36264-36267 NN denotes TEL
T21662 36268-36269 -LRB- denotes [
T21663 36272-36274 CD denotes 44
T21664 36269-36271 CD denotes 43
T21665 36271-36272 , denotes ,
T21666 36274-36275 -RRB- denotes ]
T21667 36275-36276 . denotes .
T21668 36276-36567 sentence denotes On the other hand, human lethal(3) malignant brain tumor (H-L(3)MBT) protein, which also contains a SAM domain at the C-terminus, was reported as a transcriptional repressor and the repressor activity of H-L(3)MBT required mainly the presence of the MBT repeats but not the SAM domain [45].
T21669 36277-36279 IN denotes On
T21670 36411-36419 VBN denotes reported
T21671 36280-36283 DT denotes the
T21672 36290-36294 NN denotes hand
T21673 36284-36289 JJ denotes other
T21674 36294-36296 , denotes ,
T21675 36296-36301 JJ denotes human
T21676 36346-36353 NN denotes protein
T21677 36302-36308 JJ denotes lethal
T21678 36308-36309 -LRB- denotes (
T21679 36309-36310 CD denotes 3
T21680 36310-36311 -RRB- denotes )
T21681 36312-36321 JJ denotes malignant
T21682 36328-36333 NN denotes tumor
T21683 36322-36327 NN denotes brain
T21684 36334-36335 -LRB- denotes (
T21685 36339-36340 CD denotes 3
T21686 36335-36336 NN denotes H
T21687 36336-36337 SYM denotes -
T21688 36337-36338 NN denotes L
T21689 36338-36339 -LRB- denotes (
T21690 36340-36341 -RRB- denotes )
T21691 36341-36344 NN denotes MBT
T21692 36344-36345 -RRB- denotes )
T21693 36353-36355 , denotes ,
T21694 36355-36360 WDT denotes which
T21695 36366-36374 VBZ denotes contains
T21696 36361-36365 RB denotes also
T21697 36375-36376 DT denotes a
T21698 36381-36387 NN denotes domain
T21699 36377-36380 NN denotes SAM
T21700 36388-36390 IN denotes at
T21701 36391-36394 DT denotes the
T21702 36397-36405 NN denotes terminus
T21703 36395-36396 NN denotes C
T21704 36396-36397 HYPH denotes -
T21705 36405-36407 , denotes ,
T21706 36407-36410 VBD denotes was
T21707 36420-36422 IN denotes as
T21708 36423-36424 DT denotes a
T21709 36441-36450 NN denotes repressor
T21710 36425-36440 JJ denotes transcriptional
T21711 36451-36454 CC denotes and
T21712 36455-36458 DT denotes the
T21713 36469-36477 NN denotes activity
T21714 36459-36468 NN denotes repressor
T21715 36491-36499 VBD denotes required
T21716 36478-36480 IN denotes of
T21717 36481-36482 NN denotes H
T21718 36487-36490 NN denotes MBT
T21719 36482-36483 SYM denotes -
T21720 36483-36484 NN denotes L
T21721 36484-36485 -LRB- denotes (
T21722 36485-36486 CD denotes 3
T21723 36486-36487 -RRB- denotes )
T21724 36500-36506 RB denotes mainly
T21725 36507-36510 DT denotes the
T21726 36511-36519 NN denotes presence
T21727 36520-36522 IN denotes of
T21728 36523-36526 DT denotes the
T21729 36531-36538 NNS denotes repeats
T21730 36527-36530 NN denotes MBT
T21731 36539-36542 CC denotes but
T21732 36543-36546 RB denotes not
T21733 36547-36550 DT denotes the
T21734 36555-36561 NN denotes domain
T21735 36551-36554 NN denotes SAM
T21736 36562-36563 -LRB- denotes [
T21737 36563-36565 CD denotes 45
T21738 36565-36566 -RRB- denotes ]
T21739 36566-36567 . denotes .
T21740 36567-36725 sentence denotes To determine the transcriptional repressor region of the mr-s protein, we performed a luciferase assay using site-directed mutants (DBD-W404A and DBD-G453A).
T21741 36568-36570 TO denotes To
T21742 36571-36580 VB denotes determine
T21743 36642-36651 VBD denotes performed
T21744 36581-36584 DT denotes the
T21745 36611-36617 NN denotes region
T21746 36585-36600 JJ denotes transcriptional
T21747 36601-36610 NN denotes repressor
T21748 36618-36620 IN denotes of
T21749 36621-36624 DT denotes the
T21750 36630-36637 NN denotes protein
T21751 36625-36627 NN denotes mr
T21752 36628-36629 NN denotes s
T21753 36627-36628 HYPH denotes -
T21754 36637-36639 , denotes ,
T21755 36639-36641 PRP denotes we
T21756 36652-36653 DT denotes a
T21757 36665-36670 NN denotes assay
T21758 36654-36664 NN denotes luciferase
T21759 36671-36676 VBG denotes using
T21760 36677-36681 NN denotes site
T21761 36682-36690 VBN denotes directed
T21762 36681-36682 HYPH denotes -
T21763 36691-36698 NNS denotes mutants
T21764 36699-36700 -LRB- denotes (
T21765 36704-36709 NN denotes W404A
T21766 36700-36703 NN denotes DBD
T21767 36703-36704 HYPH denotes -
T21768 36710-36713 CC denotes and
T21769 36714-36717 NN denotes DBD
T21770 36718-36723 NN denotes G453A
T21771 36717-36718 HYPH denotes -
T21772 36723-36724 -RRB- denotes )
T21773 36724-36725 . denotes .
T21774 36725-36877 sentence denotes The result showed that the reduced binding ability of self-association partially compromises the transcriptional repressive activity of mr-s (Fig. 7D).
T21775 36726-36729 DT denotes The
T21776 36730-36736 NN denotes result
T21777 36737-36743 VBD denotes showed
T21778 36744-36748 IN denotes that
T21779 36807-36818 VBZ denotes compromises
T21780 36749-36752 DT denotes the
T21781 36769-36776 NN denotes ability
T21782 36753-36760 VBN denotes reduced
T21783 36761-36768 NN denotes binding
T21784 36777-36779 IN denotes of
T21785 36780-36784 NN denotes self
T21786 36785-36796 NN denotes association
T21787 36784-36785 HYPH denotes -
T21788 36797-36806 RB denotes partially
T21789 36819-36822 DT denotes the
T21790 36850-36858 NN denotes activity
T21791 36823-36838 JJ denotes transcriptional
T21792 36839-36849 JJ denotes repressive
T21793 36859-36861 IN denotes of
T21794 36862-36864 NN denotes mr
T21795 36865-36866 NN denotes s
T21796 36864-36865 HYPH denotes -
T21797 36867-36868 -LRB- denotes (
T21798 36873-36875 NN denotes 7D
T21799 36868-36872 NN denotes Fig.
T21800 36875-36876 -RRB- denotes )
T21801 36876-36877 . denotes .
T21802 36877-37036 sentence denotes However, the repressive effect was more significant when DBD-tail, which does not contain SAM domain, was co-introduced with the 5xGAL4-pGL3 reporter plasmid.
T21803 36878-36885 RB denotes However
T21804 36909-36912 VBD denotes was
T21805 36885-36887 , denotes ,
T21806 36887-36890 DT denotes the
T21807 36902-36908 NN denotes effect
T21808 36891-36901 JJ denotes repressive
T21809 36913-36917 RBR denotes more
T21810 36918-36929 JJ denotes significant
T21811 36930-36934 WRB denotes when
T21812 36984-36997 VBN denotes co-introduced
T21813 36935-36938 NN denotes DBD
T21814 36939-36943 NN denotes tail
T21815 36938-36939 HYPH denotes -
T21816 36943-36945 , denotes ,
T21817 36945-36950 WDT denotes which
T21818 36960-36967 VB denotes contain
T21819 36951-36955 VBZ denotes does
T21820 36956-36959 RB denotes not
T21821 36968-36971 NN denotes SAM
T21822 36972-36978 NN denotes domain
T21823 36978-36980 , denotes ,
T21824 36980-36983 VBD denotes was
T21825 36998-37002 IN denotes with
T21826 37003-37006 DT denotes the
T21827 37028-37035 NN denotes plasmid
T21828 37007-37013 NN denotes 5xGAL4
T21829 37014-37018 NN denotes pGL3
T21830 37013-37014 HYPH denotes -
T21831 37019-37027 NN denotes reporter
T21832 37035-37036 . denotes .
T21833 37036-37171 sentence denotes Therefore, we conclude that a region from amino acids 463 to 542 is mainly responsible for the repressor activity in the case of mr-s.
T21834 37037-37046 RB denotes Therefore
T21835 37051-37059 VBP denotes conclude
T21836 37046-37048 , denotes ,
T21837 37048-37050 PRP denotes we
T21838 37060-37064 IN denotes that
T21839 37102-37104 VBZ denotes is
T21840 37065-37066 DT denotes a
T21841 37067-37073 NN denotes region
T21842 37074-37078 IN denotes from
T21843 37079-37084 NN denotes amino
T21844 37091-37094 CD denotes 463
T21845 37085-37090 NNS denotes acids
T21846 37095-37097 IN denotes to
T21847 37098-37101 CD denotes 542
T21848 37105-37111 RB denotes mainly
T21849 37112-37123 JJ denotes responsible
T21850 37124-37127 IN denotes for
T21851 37128-37131 DT denotes the
T21852 37142-37150 NN denotes activity
T21853 37132-37141 NN denotes repressor
T21854 37151-37153 IN denotes in
T21855 37154-37157 DT denotes the
T21856 37158-37162 NN denotes case
T21857 37163-37165 IN denotes of
T21858 37166-37168 NN denotes mr
T21859 37169-37170 NN denotes s
T21860 37168-37169 HYPH denotes -
T21861 37170-37171 . denotes .
T21862 37171-37320 sentence denotes Evolutionary conservation of the C-terminal 80 aa region of mr-s from zebrafish through human may underlie the functional importance of this region.
T21863 37172-37184 JJ denotes Evolutionary
T21864 37185-37197 NN denotes conservation
T21865 37270-37278 VB denotes underlie
T21866 37198-37200 IN denotes of
T21867 37201-37204 DT denotes the
T21868 37222-37228 NN denotes region
T21869 37205-37206 NN denotes C
T21870 37207-37215 JJ denotes terminal
T21871 37206-37207 HYPH denotes -
T21872 37216-37218 CD denotes 80
T21873 37219-37221 NN denotes aa
T21874 37229-37231 IN denotes of
T21875 37232-37234 NN denotes mr
T21876 37235-37236 NN denotes s
T21877 37234-37235 HYPH denotes -
T21878 37237-37241 IN denotes from
T21879 37242-37251 NN denotes zebrafish
T21880 37252-37259 IN denotes through
T21881 37260-37265 JJ denotes human
T21882 37266-37269 MD denotes may
T21883 37279-37282 DT denotes the
T21884 37294-37304 NN denotes importance
T21885 37283-37293 JJ denotes functional
T21886 37305-37307 IN denotes of
T21887 37308-37312 DT denotes this
T21888 37313-37319 NN denotes region
T21889 37319-37320 . denotes .
T21890 37320-37398 sentence denotes Two distinct multiprotein PcG complexes, PRC1 and PRC2, have been identified.
T21891 37321-37324 CD denotes Two
T21892 37351-37360 NNS denotes complexes
T21893 37325-37333 JJ denotes distinct
T21894 37334-37346 NN denotes multiprotein
T21895 37347-37350 NN denotes PcG
T21896 37387-37397 VBN denotes identified
T21897 37360-37362 , denotes ,
T21898 37362-37366 NN denotes PRC1
T21899 37367-37370 CC denotes and
T21900 37371-37375 NN denotes PRC2
T21901 37375-37377 , denotes ,
T21902 37377-37381 VBP denotes have
T21903 37382-37386 VBN denotes been
T21904 37397-37398 . denotes .
T21905 37398-37597 sentence denotes PRC2 is involved in the initiation of silencing and contains histone deacetylases (HDACs) and histone methyltransferases, which can methylate histone H3 lysine 9 and 27, marks of silenced chromatin.
T21906 37399-37403 NN denotes PRC2
T21907 37407-37415 VBN denotes involved
T21908 37404-37406 VBZ denotes is
T21909 37416-37418 IN denotes in
T21910 37419-37422 DT denotes the
T21911 37423-37433 NN denotes initiation
T21912 37434-37436 IN denotes of
T21913 37437-37446 NN denotes silencing
T21914 37447-37450 CC denotes and
T21915 37451-37459 VBZ denotes contains
T21916 37460-37467 NN denotes histone
T21917 37468-37480 NNS denotes deacetylases
T21918 37481-37482 -LRB- denotes (
T21919 37482-37487 NNS denotes HDACs
T21920 37487-37488 -RRB- denotes )
T21921 37489-37492 CC denotes and
T21922 37493-37500 NN denotes histone
T21923 37501-37519 NNS denotes methyltransferases
T21924 37519-37521 , denotes ,
T21925 37521-37526 WDT denotes which
T21926 37531-37540 VB denotes methylate
T21927 37527-37530 MD denotes can
T21928 37541-37548 NN denotes histone
T21929 37559-37560 CD denotes 9
T21930 37549-37551 NN denotes H3
T21931 37552-37558 NN denotes lysine
T21932 37561-37564 CC denotes and
T21933 37565-37567 CD denotes 27
T21934 37567-37569 , denotes ,
T21935 37569-37574 NNS denotes marks
T21936 37575-37577 IN denotes of
T21937 37578-37586 VBN denotes silenced
T21938 37587-37596 NN denotes chromatin
T21939 37596-37597 . denotes .
T21940 37597-37816 sentence denotes PRC1, including ph, recognizes the histone H3 lysine 27 mark set by PRC2 and maintains a stable state of gene repression in which PRC1 blocks chromatin remodeling by the trithorax group-related SWI-SNF complex [46,47].
T21941 37598-37602 NN denotes PRC1
T21942 37618-37628 VBZ denotes recognizes
T21943 37602-37604 , denotes ,
T21944 37604-37613 VBG denotes including
T21945 37614-37616 NN denotes ph
T21946 37616-37618 , denotes ,
T21947 37629-37632 DT denotes the
T21948 37654-37658 NN denotes mark
T21949 37633-37640 NN denotes histone
T21950 37641-37643 NN denotes H3
T21951 37644-37650 NN denotes lysine
T21952 37651-37653 CD denotes 27
T21953 37659-37662 VBN denotes set
T21954 37663-37665 IN denotes by
T21955 37666-37670 NN denotes PRC2
T21956 37671-37674 CC denotes and
T21957 37675-37684 VBZ denotes maintains
T21958 37685-37686 DT denotes a
T21959 37694-37699 NN denotes state
T21960 37687-37693 JJ denotes stable
T21961 37700-37702 IN denotes of
T21962 37703-37707 NN denotes gene
T21963 37708-37718 NN denotes repression
T21964 37719-37721 IN denotes in
T21965 37733-37739 VBZ denotes blocks
T21966 37722-37727 WDT denotes which
T21967 37728-37732 NN denotes PRC1
T21968 37740-37749 NN denotes chromatin
T21969 37750-37760 NN denotes remodeling
T21970 37761-37763 IN denotes by
T21971 37764-37767 DT denotes the
T21972 37800-37807 NN denotes complex
T21973 37768-37777 NN denotes trithorax
T21974 37778-37783 NN denotes group
T21975 37784-37791 VBN denotes related
T21976 37783-37784 HYPH denotes -
T21977 37792-37795 NN denotes SWI
T21978 37796-37799 NN denotes SNF
T21979 37795-37796 HYPH denotes -
T21980 37808-37809 -LRB- denotes [
T21981 37812-37814 CD denotes 47
T21982 37809-37811 CD denotes 46
T21983 37811-37812 , denotes ,
T21984 37814-37815 -RRB- denotes ]
T21985 37815-37816 . denotes .
T21986 37816-37969 sentence denotes Therefore, the mechanism of repression by PRC2 is thought to be HDAC-dependent while the mechanism of repression by PRC1 appears to be HDAC-independent.
T21987 37817-37826 RB denotes Therefore
T21988 37867-37874 VBN denotes thought
T21989 37826-37828 , denotes ,
T21990 37828-37831 DT denotes the
T21991 37832-37841 NN denotes mechanism
T21992 37842-37844 IN denotes of
T21993 37845-37855 NN denotes repression
T21994 37856-37858 IN denotes by
T21995 37859-37863 NN denotes PRC2
T21996 37864-37866 VBZ denotes is
T21997 37875-37877 TO denotes to
T21998 37878-37880 VB denotes be
T21999 37881-37885 NN denotes HDAC
T22000 37886-37895 JJ denotes dependent
T22001 37885-37886 HYPH denotes -
T22002 37896-37901 IN denotes while
T22003 37938-37945 VBZ denotes appears
T22004 37902-37905 DT denotes the
T22005 37906-37915 NN denotes mechanism
T22006 37916-37918 IN denotes of
T22007 37919-37929 NN denotes repression
T22008 37930-37932 IN denotes by
T22009 37933-37937 NN denotes PRC1
T22010 37946-37948 TO denotes to
T22011 37949-37951 VB denotes be
T22012 37952-37956 NN denotes HDAC
T22013 37957-37968 JJ denotes independent
T22014 37956-37957 HYPH denotes -
T22015 37968-37969 . denotes .
T22016 37969-38165 sentence denotes Our luciferase assay also showed that transcriptional repression of DBD-mrs was not affected by the addition of various concentrations of trichostatin A, a potent HDAC inhibitor (data not shown).
T22017 37970-37973 PRP$ denotes Our
T22018 37985-37990 NN denotes assay
T22019 37974-37984 NN denotes luciferase
T22020 37996-38002 VBD denotes showed
T22021 37991-37995 RB denotes also
T22022 38003-38007 IN denotes that
T22023 38054-38062 VBN denotes affected
T22024 38008-38023 JJ denotes transcriptional
T22025 38024-38034 NN denotes repression
T22026 38035-38037 IN denotes of
T22027 38038-38041 NN denotes DBD
T22028 38042-38045 NN denotes mrs
T22029 38041-38042 HYPH denotes -
T22030 38046-38049 VBD denotes was
T22031 38050-38053 RB denotes not
T22032 38063-38065 IN denotes by
T22033 38066-38069 DT denotes the
T22034 38070-38078 NN denotes addition
T22035 38079-38081 IN denotes of
T22036 38082-38089 JJ denotes various
T22037 38090-38104 NNS denotes concentrations
T22038 38105-38107 IN denotes of
T22039 38108-38120 NN denotes trichostatin
T22040 38121-38122 NN denotes A
T22041 38122-38124 , denotes ,
T22042 38124-38125 DT denotes a
T22043 38138-38147 NN denotes inhibitor
T22044 38126-38132 JJ denotes potent
T22045 38133-38137 NN denotes HDAC
T22046 38148-38149 -LRB- denotes (
T22047 38158-38163 VBN denotes shown
T22048 38149-38153 NNS denotes data
T22049 38154-38157 RB denotes not
T22050 38163-38164 -RRB- denotes )
T22051 38164-38165 . denotes .
T22052 38165-38296 sentence denotes Therefore, we speculate that the mechanism of repression of mr-s may be HDAC-independent and more similar to that of PRC1 complex.
T22053 38166-38175 RB denotes Therefore
T22054 38180-38189 VBP denotes speculate
T22055 38175-38177 , denotes ,
T22056 38177-38179 PRP denotes we
T22057 38190-38194 IN denotes that
T22058 38235-38237 VB denotes be
T22059 38195-38198 DT denotes the
T22060 38199-38208 NN denotes mechanism
T22061 38209-38211 IN denotes of
T22062 38212-38222 NN denotes repression
T22063 38223-38225 IN denotes of
T22064 38226-38228 NN denotes mr
T22065 38229-38230 NN denotes s
T22066 38228-38229 HYPH denotes -
T22067 38231-38234 MD denotes may
T22068 38238-38242 NN denotes HDAC
T22069 38243-38254 JJ denotes independent
T22070 38242-38243 HYPH denotes -
T22071 38255-38258 CC denotes and
T22072 38259-38263 RBR denotes more
T22073 38264-38271 JJ denotes similar
T22074 38272-38274 IN denotes to
T22075 38275-38279 DT denotes that
T22076 38280-38282 IN denotes of
T22077 38283-38287 NN denotes PRC1
T22078 38288-38295 NN denotes complex
T22079 38295-38296 . denotes .
T22080 38296-38547 sentence denotes In the present study, the biochemical experiments demonstrate that the mr-s protein functions as a transcriptional repressor and possibly down-regulates the spatial and temporal expression of the target genes during retinal photoreceptor development.
T22081 38297-38299 IN denotes In
T22082 38347-38358 VBP denotes demonstrate
T22083 38300-38303 DT denotes the
T22084 38312-38317 NN denotes study
T22085 38304-38311 JJ denotes present
T22086 38317-38319 , denotes ,
T22087 38319-38322 DT denotes the
T22088 38335-38346 NNS denotes experiments
T22089 38323-38334 JJ denotes biochemical
T22090 38359-38363 IN denotes that
T22091 38381-38390 VBZ denotes functions
T22092 38364-38367 DT denotes the
T22093 38373-38380 NN denotes protein
T22094 38368-38370 NN denotes mr
T22095 38371-38372 NN denotes s
T22096 38370-38371 HYPH denotes -
T22097 38391-38393 IN denotes as
T22098 38394-38395 DT denotes a
T22099 38412-38421 NN denotes repressor
T22100 38396-38411 JJ denotes transcriptional
T22101 38422-38425 CC denotes and
T22102 38426-38434 RB denotes possibly
T22103 38440-38449 VBZ denotes regulates
T22104 38435-38439 RB denotes down
T22105 38439-38440 HYPH denotes -
T22106 38450-38453 DT denotes the
T22107 38475-38485 NN denotes expression
T22108 38454-38461 JJ denotes spatial
T22109 38462-38465 CC denotes and
T22110 38466-38474 JJ denotes temporal
T22111 38486-38488 IN denotes of
T22112 38489-38492 DT denotes the
T22113 38500-38505 NNS denotes genes
T22114 38493-38499 NN denotes target
T22115 38506-38512 IN denotes during
T22116 38513-38520 JJ denotes retinal
T22117 38535-38546 NN denotes development
T22118 38521-38534 NN denotes photoreceptor
T22119 38546-38547 . denotes .
T22120 38547-38672 sentence denotes Our data also revealed that the repressive activity of mr-s is mainly due to the C-terminal region (amino acids 463 to 542).
T22121 38548-38551 PRP$ denotes Our
T22122 38552-38556 NNS denotes data
T22123 38562-38570 VBD denotes revealed
T22124 38557-38561 RB denotes also
T22125 38571-38575 IN denotes that
T22126 38608-38610 VBZ denotes is
T22127 38576-38579 DT denotes the
T22128 38591-38599 NN denotes activity
T22129 38580-38590 JJ denotes repressive
T22130 38600-38602 IN denotes of
T22131 38603-38605 NN denotes mr
T22132 38606-38607 NN denotes s
T22133 38605-38606 HYPH denotes -
T22134 38611-38617 RB denotes mainly
T22135 38618-38621 IN denotes due
T22136 38622-38624 IN denotes to
T22137 38625-38628 DT denotes the
T22138 38640-38646 NN denotes region
T22139 38629-38630 NN denotes C
T22140 38631-38639 JJ denotes terminal
T22141 38630-38631 HYPH denotes -
T22142 38647-38648 -LRB- denotes (
T22143 38660-38663 CD denotes 463
T22144 38648-38653 NN denotes amino
T22145 38654-38659 NNS denotes acids
T22146 38664-38666 IN denotes to
T22147 38667-38670 CD denotes 542
T22148 38670-38671 -RRB- denotes )
T22149 38671-38672 . denotes .
T22150 38672-38730 sentence denotes However, downstream targets of mr-s still remain unclear.
T22151 38673-38680 RB denotes However
T22152 38715-38721 VBP denotes remain
T22153 38680-38682 , denotes ,
T22154 38682-38692 JJ denotes downstream
T22155 38693-38700 NNS denotes targets
T22156 38701-38703 IN denotes of
T22157 38704-38706 NN denotes mr
T22158 38707-38708 NN denotes s
T22159 38706-38707 HYPH denotes -
T22160 38709-38714 RB denotes still
T22161 38722-38729 JJ denotes unclear
T22162 38729-38730 . denotes .
T22163 38730-38868 sentence denotes In situ hybridization showed that the peak of mr-s expression is around P6, when retinal photoreceptors undergo terminal differentiation.
T22164 38731-38733 FW denotes In
T22165 38734-38738 FW denotes situ
T22166 38739-38752 NN denotes hybridization
T22167 38753-38759 VBD denotes showed
T22168 38760-38764 IN denotes that
T22169 38793-38795 VBZ denotes is
T22170 38765-38768 DT denotes the
T22171 38769-38773 NN denotes peak
T22172 38774-38776 IN denotes of
T22173 38777-38779 NN denotes mr
T22174 38780-38781 NN denotes s
T22175 38779-38780 HYPH denotes -
T22176 38782-38792 NN denotes expression
T22177 38796-38802 RB denotes around
T22178 38803-38805 NN denotes P6
T22179 38805-38807 , denotes ,
T22180 38807-38811 WRB denotes when
T22181 38835-38842 VBP denotes undergo
T22182 38812-38819 JJ denotes retinal
T22183 38820-38834 NNS denotes photoreceptors
T22184 38843-38851 JJ denotes terminal
T22185 38852-38867 NN denotes differentiation
T22186 38867-38868 . denotes .
T22187 38868-38947 sentence denotes We hypothesize that the target genes of mr-s might be non-photoreceptor genes.
T22188 38869-38871 PRP denotes We
T22189 38872-38883 VBP denotes hypothesize
T22190 38884-38888 IN denotes that
T22191 38920-38922 VB denotes be
T22192 38889-38892 DT denotes the
T22193 38900-38905 NNS denotes genes
T22194 38893-38899 NN denotes target
T22195 38906-38908 IN denotes of
T22196 38909-38911 NN denotes mr
T22197 38912-38913 NN denotes s
T22198 38911-38912 HYPH denotes -
T22199 38914-38919 MD denotes might
T22200 38923-38940 JJ denotes non-photoreceptor
T22201 38941-38946 NNS denotes genes
T22202 38946-38947 . denotes .
T22203 38947-39053 sentence denotes In this case, mr-s may suppress the expression of non-photoreceptor genes in rod and cone photoreceptors.
T22204 38948-38950 IN denotes In
T22205 38971-38979 VB denotes suppress
T22206 38951-38955 DT denotes this
T22207 38956-38960 NN denotes case
T22208 38960-38962 , denotes ,
T22209 38962-38964 NN denotes mr
T22210 38965-38966 NN denotes s
T22211 38964-38965 HYPH denotes -
T22212 38967-38970 MD denotes may
T22213 38980-38983 DT denotes the
T22214 38984-38994 NN denotes expression
T22215 38995-38997 IN denotes of
T22216 38998-39015 JJ denotes non-photoreceptor
T22217 39016-39021 NNS denotes genes
T22218 39022-39024 IN denotes in
T22219 39025-39028 NN denotes rod
T22220 39038-39052 NNS denotes photoreceptors
T22221 39029-39032 CC denotes and
T22222 39033-39037 NN denotes cone
T22223 39052-39053 . denotes .
T22224 39053-39185 sentence denotes There may be another possibility that mr-s is involved in cell fate determination of rod photoreceptors versus cone photoreceptors.
T22225 39054-39059 EX denotes There
T22226 39064-39066 VB denotes be
T22227 39060-39063 MD denotes may
T22228 39067-39074 DT denotes another
T22229 39075-39086 NN denotes possibility
T22230 39087-39091 IN denotes that
T22231 39100-39108 VBN denotes involved
T22232 39092-39094 NN denotes mr
T22233 39095-39096 NN denotes s
T22234 39094-39095 HYPH denotes -
T22235 39097-39099 VBZ denotes is
T22236 39109-39111 IN denotes in
T22237 39112-39116 NN denotes cell
T22238 39117-39121 NN denotes fate
T22239 39122-39135 NN denotes determination
T22240 39136-39138 IN denotes of
T22241 39139-39142 NN denotes rod
T22242 39143-39157 NNS denotes photoreceptors
T22243 39158-39164 CC denotes versus
T22244 39165-39169 NN denotes cone
T22245 39170-39184 NNS denotes photoreceptors
T22246 39184-39185 . denotes .
T22247 39185-39371 sentence denotes While cone photoreceptors are born during the early embryonic stages of mouse retinogenesis, rod photoreceptors are born primarily in the late embryonic and early postnatal period [48].
T22248 39186-39191 IN denotes While
T22249 39216-39220 VBN denotes born
T22250 39192-39196 NN denotes cone
T22251 39197-39211 NNS denotes photoreceptors
T22252 39212-39215 VBP denotes are
T22253 39302-39306 VBN denotes born
T22254 39221-39227 IN denotes during
T22255 39228-39231 DT denotes the
T22256 39248-39254 NNS denotes stages
T22257 39232-39237 JJ denotes early
T22258 39238-39247 JJ denotes embryonic
T22259 39255-39257 IN denotes of
T22260 39258-39263 NN denotes mouse
T22261 39264-39277 NN denotes retinogenesis
T22262 39277-39279 , denotes ,
T22263 39279-39282 NN denotes rod
T22264 39283-39297 NNS denotes photoreceptors
T22265 39298-39301 VBP denotes are
T22266 39307-39316 RB denotes primarily
T22267 39317-39319 IN denotes in
T22268 39320-39323 DT denotes the
T22269 39359-39365 NN denotes period
T22270 39324-39328 JJ denotes late
T22271 39329-39338 JJ denotes embryonic
T22272 39339-39342 CC denotes and
T22273 39343-39348 JJ denotes early
T22274 39349-39358 JJ denotes postnatal
T22275 39366-39367 -LRB- denotes [
T22276 39367-39369 CD denotes 48
T22277 39369-39370 -RRB- denotes ]
T22278 39370-39371 . denotes .
T22279 39371-39621 sentence denotes The expression pattern of mr-s may suggest that mr-s is expressed in rod photoreceptors but not in cone photoreceptors as well as Nr2e3, which is known as a transcriptional repressor and is thought to down-regulate cone photoreceptor-specific genes.
T22280 39372-39375 DT denotes The
T22281 39387-39394 NN denotes pattern
T22282 39376-39386 NN denotes expression
T22283 39407-39414 VB denotes suggest
T22284 39395-39397 IN denotes of
T22285 39398-39400 NN denotes mr
T22286 39401-39402 NN denotes s
T22287 39400-39401 HYPH denotes -
T22288 39403-39406 MD denotes may
T22289 39415-39419 IN denotes that
T22290 39428-39437 VBN denotes expressed
T22291 39420-39422 NN denotes mr
T22292 39423-39424 NN denotes s
T22293 39422-39423 HYPH denotes -
T22294 39425-39427 VBZ denotes is
T22295 39438-39440 IN denotes in
T22296 39441-39444 NN denotes rod
T22297 39445-39459 NNS denotes photoreceptors
T22298 39460-39463 CC denotes but
T22299 39464-39467 RB denotes not
T22300 39468-39470 IN denotes in
T22301 39471-39475 NN denotes cone
T22302 39476-39490 NNS denotes photoreceptors
T22303 39491-39493 RB denotes as
T22304 39499-39501 IN denotes as
T22305 39494-39498 RB denotes well
T22306 39502-39507 NN denotes Nr2e3
T22307 39507-39509 , denotes ,
T22308 39509-39514 WDT denotes which
T22309 39518-39523 VBN denotes known
T22310 39515-39517 VBZ denotes is
T22311 39524-39526 IN denotes as
T22312 39527-39528 DT denotes a
T22313 39545-39554 NN denotes repressor
T22314 39529-39544 JJ denotes transcriptional
T22315 39555-39558 CC denotes and
T22316 39559-39561 VBZ denotes is
T22317 39562-39569 VBN denotes thought
T22318 39570-39572 TO denotes to
T22319 39578-39586 VB denotes regulate
T22320 39573-39577 RB denotes down
T22321 39577-39578 HYPH denotes -
T22322 39587-39591 NN denotes cone
T22323 39615-39620 NNS denotes genes
T22324 39592-39605 NN denotes photoreceptor
T22325 39606-39614 JJ denotes specific
T22326 39605-39606 HYPH denotes -
T22327 39620-39621 . denotes .
T22328 39621-39740 sentence denotes To clarify the biological function of mr-s, analysis of mice with targeted disruptions of mr-s will be very important.
T22329 39622-39624 TO denotes To
T22330 39625-39632 VB denotes clarify
T22331 39722-39724 VB denotes be
T22332 39633-39636 DT denotes the
T22333 39648-39656 NN denotes function
T22334 39637-39647 JJ denotes biological
T22335 39657-39659 IN denotes of
T22336 39660-39662 NN denotes mr
T22337 39663-39664 NN denotes s
T22338 39662-39663 HYPH denotes -
T22339 39664-39666 , denotes ,
T22340 39666-39674 NN denotes analysis
T22341 39675-39677 IN denotes of
T22342 39678-39682 NNS denotes mice
T22343 39683-39687 IN denotes with
T22344 39688-39696 VBN denotes targeted
T22345 39697-39708 NNS denotes disruptions
T22346 39709-39711 IN denotes of
T22347 39712-39714 NN denotes mr
T22348 39715-39716 NN denotes s
T22349 39714-39715 HYPH denotes -
T22350 39717-39721 MD denotes will
T22351 39725-39729 RB denotes very
T22352 39730-39739 JJ denotes important
T22353 39739-39740 . denotes .
T22676 39753-39757 RB denotes Here
T22677 39761-39771 VBD denotes identified
T22678 39758-39760 PRP denotes we
T22679 39772-39777 NN denotes mouse
T22680 39781-39782 NN denotes s
T22681 39778-39780 NN denotes mr
T22682 39780-39781 HYPH denotes -
T22683 39782-39784 , denotes ,
T22684 39784-39789 WDT denotes which
T22685 39807-39816 VBN denotes expressed
T22686 39790-39792 VBZ denotes is
T22687 39793-39806 RB denotes predominantly
T22688 39817-39819 IN denotes in
T22689 39820-39827 JJ denotes retinal
T22690 39828-39842 NNS denotes photoreceptors
T22691 39843-39846 CC denotes and
T22692 39847-39850 DT denotes the
T22693 39858-39863 NN denotes gland
T22694 39851-39857 JJ denotes pineal
T22695 39863-39864 . denotes .
T22696 39864-39998 sentence denotes mr-s is evolutionarily conserved from zebrafish through to human, suggesting a significant role of mr-s in photoreceptor development.
T22697 39865-39867 NN denotes mr
T22698 39868-39869 NN denotes s
T22699 39867-39868 HYPH denotes -
T22700 39888-39897 VBN denotes conserved
T22701 39870-39872 VBZ denotes is
T22702 39873-39887 RB denotes evolutionarily
T22703 39898-39902 IN denotes from
T22704 39903-39912 NN denotes zebrafish
T22705 39913-39920 IN denotes through
T22706 39921-39923 IN denotes to
T22707 39924-39929 JJ denotes human
T22708 39929-39931 , denotes ,
T22709 39931-39941 VBG denotes suggesting
T22710 39942-39943 DT denotes a
T22711 39956-39960 NN denotes role
T22712 39944-39955 JJ denotes significant
T22713 39961-39963 IN denotes of
T22714 39964-39966 NN denotes mr
T22715 39967-39968 NN denotes s
T22716 39966-39967 HYPH denotes -
T22717 39969-39971 IN denotes in
T22718 39972-39985 NN denotes photoreceptor
T22719 39986-39997 NN denotes development
T22720 39997-39998 . denotes .
T22721 39998-40121 sentence denotes Our present data suggest that mr-s protein localizes in the nucleus and can self-associated mainly through the SAM domain.
T22722 39999-40002 PRP$ denotes Our
T22723 40011-40015 NNS denotes data
T22724 40003-40010 JJ denotes present
T22725 40016-40023 VBP denotes suggest
T22726 40024-40028 IN denotes that
T22727 40042-40051 VBZ denotes localizes
T22728 40029-40031 NN denotes mr
T22729 40032-40033 NN denotes s
T22730 40031-40032 HYPH denotes -
T22731 40034-40041 NN denotes protein
T22732 40052-40054 IN denotes in
T22733 40055-40058 DT denotes the
T22734 40059-40066 NN denotes nucleus
T22735 40067-40070 CC denotes and
T22736 40071-40074 MD denotes can
T22737 40080-40090 VBN denotes associated
T22738 40075-40079 NN denotes self
T22739 40079-40080 HYPH denotes -
T22740 40091-40097 RB denotes mainly
T22741 40098-40105 IN denotes through
T22742 40106-40109 DT denotes the
T22743 40114-40120 NN denotes domain
T22744 40110-40113 NN denotes SAM
T22745 40120-40121 . denotes .
T22746 40121-40204 sentence denotes Moreover, mr-s protein fused to GAL4 DBD functions as a transcriptional repressor.
T22747 40122-40130 RB denotes Moreover
T22748 40163-40172 VBZ denotes functions
T22749 40130-40132 , denotes ,
T22750 40132-40134 NN denotes mr
T22751 40135-40136 NN denotes s
T22752 40134-40135 HYPH denotes -
T22753 40137-40144 NN denotes protein
T22754 40145-40150 VBN denotes fused
T22755 40151-40153 IN denotes to
T22756 40154-40158 NN denotes GAL4
T22757 40159-40162 NN denotes DBD
T22758 40173-40175 IN denotes as
T22759 40176-40177 DT denotes a
T22760 40194-40203 NN denotes repressor
T22761 40178-40193 JJ denotes transcriptional
T22762 40203-40204 . denotes .
T22763 40204-40293 sentence denotes The repressive activity of mr-s is due to its C-terminal region (amino acid 463 to 542).
T22764 40205-40208 DT denotes The
T22765 40220-40228 NN denotes activity
T22766 40209-40219 JJ denotes repressive
T22767 40237-40239 VBZ denotes is
T22768 40229-40231 IN denotes of
T22769 40232-40234 NN denotes mr
T22770 40235-40236 NN denotes s
T22771 40234-40235 HYPH denotes -
T22772 40240-40243 IN denotes due
T22773 40244-40246 IN denotes to
T22774 40247-40250 PRP$ denotes its
T22775 40262-40268 NN denotes region
T22776 40251-40252 NN denotes C
T22777 40253-40261 JJ denotes terminal
T22778 40252-40253 HYPH denotes -
T22779 40269-40270 -LRB- denotes (
T22780 40281-40284 CD denotes 463
T22781 40270-40275 NN denotes amino
T22782 40276-40280 NN denotes acid
T22783 40285-40287 IN denotes to
T22784 40288-40291 CD denotes 542
T22785 40291-40292 -RRB- denotes )
T22786 40292-40293 . denotes .
T22787 40293-40425 sentence denotes Taken together, mr-s is a novel repressor molecule possibly involved in the development of retinal photoreceptors and pineal gland.
T22788 40294-40299 VBN denotes Taken
T22789 40315-40317 VBZ denotes is
T22790 40300-40308 RB denotes together
T22791 40308-40310 , denotes ,
T22792 40310-40312 NN denotes mr
T22793 40313-40314 NN denotes s
T22794 40312-40313 HYPH denotes -
T22795 40318-40319 DT denotes a
T22796 40336-40344 NN denotes molecule
T22797 40320-40325 JJ denotes novel
T22798 40326-40335 NN denotes repressor
T22799 40345-40353 RB denotes possibly
T22800 40354-40362 VBN denotes involved
T22801 40363-40365 IN denotes in
T22802 40366-40369 DT denotes the
T22803 40370-40381 NN denotes development
T22804 40382-40384 IN denotes of
T22805 40385-40392 JJ denotes retinal
T22806 40393-40407 NNS denotes photoreceptors
T22807 40408-40411 CC denotes and
T22808 40412-40418 JJ denotes pineal
T22809 40419-40424 NN denotes gland
T22810 40424-40425 . denotes .
T23217 40436-40445 NN denotes Isolation
T23218 40446-40448 IN denotes of
T23219 40449-40454 NN denotes mouse
T23220 40460-40464 NN denotes cDNA
T23221 40455-40457 NN denotes mr
T23222 40458-40459 NN denotes s
T23223 40457-40458 HYPH denotes -
T23224 40464-40611 sentence denotes We used the bioinformatics method Digital Differential Display (NCBI, UniGene) to screen novel mouse genes expressed preferentially in the retina.
T23225 40465-40467 PRP denotes We
T23226 40468-40472 VBD denotes used
T23227 40473-40476 DT denotes the
T23228 40492-40498 NN denotes method
T23229 40477-40491 NN denotes bioinformatics
T23230 40499-40506 NNP denotes Digital
T23231 40520-40527 NNP denotes Display
T23232 40507-40519 NNP denotes Differential
T23233 40528-40529 -LRB- denotes (
T23234 40529-40533 NNP denotes NCBI
T23235 40533-40535 , denotes ,
T23236 40535-40542 NNP denotes UniGene
T23237 40542-40543 -RRB- denotes )
T23238 40544-40546 TO denotes to
T23239 40547-40553 VB denotes screen
T23240 40554-40559 JJ denotes novel
T23241 40566-40571 NNS denotes genes
T23242 40560-40565 NN denotes mouse
T23243 40572-40581 VBN denotes expressed
T23244 40582-40596 RB denotes preferentially
T23245 40597-40599 IN denotes in
T23246 40600-40603 DT denotes the
T23247 40604-40610 NN denotes retina
T23248 40610-40611 . denotes .
T23249 40611-40694 sentence denotes Some of the clusters in the UniGene database were mainly from mouse retinal cDNAs.
T23250 40612-40616 DT denotes Some
T23251 40657-40661 VBD denotes were
T23252 40617-40619 IN denotes of
T23253 40620-40623 DT denotes the
T23254 40624-40632 NNS denotes clusters
T23255 40633-40635 IN denotes in
T23256 40636-40639 DT denotes the
T23257 40648-40656 NN denotes database
T23258 40640-40647 NNP denotes UniGene
T23259 40662-40668 RB denotes mainly
T23260 40669-40673 IN denotes from
T23261 40674-40679 NN denotes mouse
T23262 40688-40693 NNS denotes cDNAs
T23263 40680-40687 JJ denotes retinal
T23264 40693-40694 . denotes .
T23265 40694-40792 sentence denotes One clone in these clusters (#Mm. 246385) has a weak homology with the polyhomeotic family genes.
T23266 40695-40698 CD denotes One
T23267 40699-40704 NN denotes clone
T23268 40737-40740 VBZ denotes has
T23269 40705-40707 IN denotes in
T23270 40708-40713 DT denotes these
T23271 40714-40722 NNS denotes clusters
T23272 40723-40724 -LRB- denotes (
T23273 40725-40728 NNP denotes Mm.
T23274 40724-40725 SYM denotes #
T23275 40729-40735 CD denotes 246385
T23276 40735-40736 -RRB- denotes )
T23277 40741-40742 DT denotes a
T23278 40748-40756 NN denotes homology
T23279 40743-40747 JJ denotes weak
T23280 40757-40761 IN denotes with
T23281 40762-40765 DT denotes the
T23282 40786-40791 NNS denotes genes
T23283 40766-40778 JJ denotes polyhomeotic
T23284 40779-40785 NN denotes family
T23285 40791-40792 . denotes .
T23286 40792-40911 sentence denotes A 735-bp cDNA fragment of this clone, encoding amino acids 140–384 was amplified by RT-PCR from mouse P0 retinal cDNA.
T23287 40793-40794 DT denotes A
T23288 40807-40815 NN denotes fragment
T23289 40795-40798 CD denotes 735
T23290 40799-40801 NN denotes bp
T23291 40798-40799 HYPH denotes -
T23292 40802-40806 NN denotes cDNA
T23293 40864-40873 VBN denotes amplified
T23294 40816-40818 IN denotes of
T23295 40819-40823 DT denotes this
T23296 40824-40829 NN denotes clone
T23297 40829-40831 , denotes ,
T23298 40831-40839 VBG denotes encoding
T23299 40840-40845 NN denotes amino
T23300 40852-40855 CD denotes 140
T23301 40846-40851 NNS denotes acids
T23302 40855-40856 SYM denotes –
T23303 40856-40859 CD denotes 384
T23304 40860-40863 VBD denotes was
T23305 40874-40876 IN denotes by
T23306 40877-40879 NN denotes RT
T23307 40880-40883 NN denotes PCR
T23308 40879-40880 HYPH denotes -
T23309 40884-40888 IN denotes from
T23310 40889-40894 NN denotes mouse
T23311 40906-40910 NN denotes cDNA
T23312 40895-40897 NN denotes P0
T23313 40898-40905 JJ denotes retinal
T23314 40910-40911 . denotes .
T23315 40911-41020 sentence denotes This fragment was used as the probe for library screening, in situ hybridization and Northern hybridization.
T23316 40912-40916 DT denotes This
T23317 40917-40925 NN denotes fragment
T23318 40930-40934 VBN denotes used
T23319 40926-40929 VBD denotes was
T23320 40935-40937 IN denotes as
T23321 40938-40941 DT denotes the
T23322 40942-40947 NN denotes probe
T23323 40948-40951 IN denotes for
T23324 40952-40959 NN denotes library
T23325 40960-40969 NN denotes screening
T23326 40969-40971 , denotes ,
T23327 40971-40973 FW denotes in
T23328 40974-40978 FW denotes situ
T23329 40979-40992 NN denotes hybridization
T23330 40993-40996 CC denotes and
T23331 40997-41005 NNP denotes Northern
T23332 41006-41019 NN denotes hybridization
T23333 41019-41020 . denotes .
T23334 41020-41100 sentence denotes A mouse P0-P3 retinal cDNA library was screened using this mouse cDNA fragment.
T23335 41021-41022 DT denotes A
T23336 41048-41055 NN denotes library
T23337 41023-41028 NN denotes mouse
T23338 41029-41031 NN denotes P0
T23339 41031-41032 SYM denotes -
T23340 41032-41034 NN denotes P3
T23341 41035-41042 JJ denotes retinal
T23342 41043-41047 NN denotes cDNA
T23343 41060-41068 VBN denotes screened
T23344 41056-41059 VBD denotes was
T23345 41069-41074 VBG denotes using
T23346 41075-41079 DT denotes this
T23347 41091-41099 NN denotes fragment
T23348 41080-41085 NN denotes mouse
T23349 41086-41090 NN denotes cDNA
T23350 41099-41100 . denotes .
T23351 41100-41224 sentence denotes Positive bacteriophage clones were isolated and the full-length mr-s fragment was inserted into pBluescriptII (Stratagene).
T23352 41101-41109 JJ denotes Positive
T23353 41124-41130 NNS denotes clones
T23354 41110-41123 NN denotes bacteriophage
T23355 41136-41144 VBN denotes isolated
T23356 41131-41135 VBD denotes were
T23357 41145-41148 CC denotes and
T23358 41149-41152 DT denotes the
T23359 41170-41178 NN denotes fragment
T23360 41153-41157 JJ denotes full
T23361 41158-41164 NN denotes length
T23362 41157-41158 HYPH denotes -
T23363 41165-41167 NN denotes mr
T23364 41168-41169 NN denotes s
T23365 41167-41168 HYPH denotes -
T23366 41183-41191 VBN denotes inserted
T23367 41179-41182 VBD denotes was
T23368 41192-41196 IN denotes into
T23369 41197-41210 NNP denotes pBluescriptII
T23370 41211-41212 -LRB- denotes (
T23371 41212-41222 NNP denotes Stratagene
T23372 41222-41223 -RRB- denotes )
T23373 41223-41224 . denotes .
T23374 41224-41301 sentence denotes DNA sequencing was performed on both strands by the cycle sequencing method.
T23375 41225-41228 NN denotes DNA
T23376 41229-41239 NN denotes sequencing
T23377 41244-41253 VBN denotes performed
T23378 41240-41243 VBD denotes was
T23379 41254-41256 IN denotes on
T23380 41257-41261 DT denotes both
T23381 41262-41269 NNS denotes strands
T23382 41270-41272 IN denotes by
T23383 41273-41276 DT denotes the
T23384 41294-41300 NN denotes method
T23385 41277-41282 NN denotes cycle
T23386 41283-41293 NN denotes sequencing
T23387 41300-41301 . denotes .
T23388 41301-41424 sentence denotes The nucleotide sequence for mr-s gene has been deposited in the GenBank database under GenBank Accession Number #AY458844.
T23389 41302-41305 DT denotes The
T23390 41317-41325 NN denotes sequence
T23391 41306-41316 NN denotes nucleotide
T23392 41349-41358 VBN denotes deposited
T23393 41326-41329 IN denotes for
T23394 41330-41332 NN denotes mr
T23395 41333-41334 NN denotes s
T23396 41332-41333 HYPH denotes -
T23397 41335-41339 NN denotes gene
T23398 41340-41343 VBZ denotes has
T23399 41344-41348 VBN denotes been
T23400 41359-41361 IN denotes in
T23401 41362-41365 DT denotes the
T23402 41374-41382 NN denotes database
T23403 41366-41373 NNP denotes GenBank
T23404 41383-41388 IN denotes under
T23405 41389-41396 NNP denotes GenBank
T23406 41415-41423 NN denotes AY458844
T23407 41397-41406 NNP denotes Accession
T23408 41407-41413 NNP denotes Number
T23409 41414-41415 SYM denotes #
T23410 41423-41424 . denotes .
T23508 41426-41428 FW denotes In
T23509 41429-41433 FW denotes situ
T23510 41434-41447 NN denotes hybridization
T23511 41447-41547 sentence denotes The 735-bp cDNA fragment of mr-s amplified by RT-PCR was used as a probe for in situ hybridization.
T23512 41448-41451 DT denotes The
T23513 41464-41472 NN denotes fragment
T23514 41452-41455 CD denotes 735
T23515 41456-41458 NN denotes bp
T23516 41455-41456 HYPH denotes -
T23517 41459-41463 NN denotes cDNA
T23518 41505-41509 VBN denotes used
T23519 41473-41475 IN denotes of
T23520 41476-41478 NN denotes mr
T23521 41479-41480 NN denotes s
T23522 41478-41479 HYPH denotes -
T23523 41481-41490 VBN denotes amplified
T23524 41491-41493 IN denotes by
T23525 41494-41496 NN denotes RT
T23526 41497-41500 NN denotes PCR
T23527 41496-41497 HYPH denotes -
T23528 41501-41504 VBD denotes was
T23529 41510-41512 IN denotes as
T23530 41513-41514 DT denotes a
T23531 41515-41520 NN denotes probe
T23532 41521-41524 IN denotes for
T23533 41525-41527 FW denotes in
T23534 41528-41532 FW denotes situ
T23535 41533-41546 NN denotes hybridization
T23536 41546-41547 . denotes .
T23537 41547-41613 sentence denotes In situ hybridization was performed as described previously [49].
T23538 41548-41550 FW denotes In
T23539 41551-41555 FW denotes situ
T23540 41556-41569 NN denotes hybridization
T23541 41574-41583 VBN denotes performed
T23542 41570-41573 VBD denotes was
T23543 41584-41586 IN denotes as
T23544 41587-41596 VBN denotes described
T23545 41597-41607 RB denotes previously
T23546 41608-41609 -LRB- denotes [
T23547 41609-41611 CD denotes 49
T23548 41611-41612 -RRB- denotes ]
T23549 41612-41613 . denotes .
T23802 41615-41619 NN denotes Cell
T23803 41620-41627 NN denotes culture
T23804 41628-41631 CC denotes and
T23805 41632-41644 NN denotes transfection
T23806 41644-41826 sentence denotes HEK293T cells were maintained at 37°C in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (Sigma), 100 IU/ml penicillin and 100 μg/ml streptomycin.
T23807 41645-41652 NN denotes HEK293T
T23808 41653-41658 NNS denotes cells
T23809 41664-41674 VBN denotes maintained
T23810 41659-41663 VBD denotes were
T23811 41675-41677 IN denotes at
T23812 41678-41680 CD denotes 37
T23813 41680-41682 NN denotes °C
T23814 41683-41685 IN denotes in
T23815 41686-41694 NNP denotes Dulbecco
T23816 41714-41720 NN denotes medium
T23817 41694-41696 POS denotes 's
T23818 41697-41705 VBN denotes modified
T23819 41706-41711 NNP denotes Eagle
T23820 41711-41713 POS denotes 's
T23821 41721-41722 -LRB- denotes (
T23822 41722-41726 NN denotes DMEM
T23823 41726-41727 -RRB- denotes )
T23824 41728-41740 VBN denotes supplemented
T23825 41741-41745 IN denotes with
T23826 41746-41748 CD denotes 10
T23827 41748-41749 NN denotes %
T23828 41763-41768 NN denotes serum
T23829 41750-41755 JJ denotes fetal
T23830 41756-41762 JJ denotes bovine
T23831 41769-41770 -LRB- denotes (
T23832 41770-41775 NNP denotes Sigma
T23833 41775-41776 -RRB- denotes )
T23834 41776-41778 , denotes ,
T23835 41778-41781 CD denotes 100
T23836 41782-41784 NN denotes IU
T23837 41788-41798 NN denotes penicillin
T23838 41784-41785 SYM denotes /
T23839 41785-41787 NN denotes ml
T23840 41799-41802 CC denotes and
T23841 41803-41806 CD denotes 100
T23842 41807-41809 NN denotes μg
T23843 41813-41825 NN denotes streptomycin
T23844 41809-41810 SYM denotes /
T23845 41810-41812 NN denotes ml
T23846 41825-41826 . denotes .
T23847 41826-41954 sentence denotes Transient transfection of HEK293T cells was carried out using calcium phosphate method or Fugene6 transfection reagent (Roche).
T23848 41827-41836 JJ denotes Transient
T23849 41837-41849 NN denotes transfection
T23850 41871-41878 VBN denotes carried
T23851 41850-41852 IN denotes of
T23852 41853-41860 NN denotes HEK293T
T23853 41861-41866 NNS denotes cells
T23854 41867-41870 VBD denotes was
T23855 41879-41882 RP denotes out
T23856 41883-41888 VBG denotes using
T23857 41889-41896 NN denotes calcium
T23858 41897-41906 NN denotes phosphate
T23859 41907-41913 NN denotes method
T23860 41914-41916 CC denotes or
T23861 41917-41924 NN denotes Fugene6
T23862 41938-41945 NN denotes reagent
T23863 41925-41937 NN denotes transfection
T23864 41946-41947 -LRB- denotes (
T23865 41947-41952 NNP denotes Roche
T23866 41952-41953 -RRB- denotes )
T23867 41953-41954 . denotes .
T23868 41954-42113 sentence denotes Y79 retinoblastoma cells were maintained in Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 20% FBS.
T23869 41955-41958 NN denotes Y79
T23870 41974-41979 NNS denotes cells
T23871 41959-41973 NN denotes retinoblastoma
T23872 41985-41995 VBN denotes maintained
T23873 41980-41984 VBD denotes were
T23874 41996-41998 IN denotes in
T23875 41999-42005 NNP denotes Iscove
T23876 42028-42034 NN denotes medium
T23877 42005-42007 POS denotes 's
T23878 42008-42016 VBN denotes modified
T23879 42017-42025 NNP denotes Dulbecco
T23880 42025-42027 POS denotes 's
T23881 42035-42039 IN denotes with
T23882 42040-42041 CD denotes 4
T23883 42042-42044 NN denotes mM
T23884 42047-42056 NN denotes glutamine
T23885 42045-42046 NN denotes L
T23886 42046-42047 HYPH denotes -
T23887 42057-42065 VBN denotes adjusted
T23888 42066-42068 TO denotes to
T23889 42069-42076 VB denotes contain
T23890 42077-42080 CD denotes 1.5
T23891 42081-42082 NN denotes g
T23892 42092-42103 NN denotes bicarbonate
T23893 42082-42083 SYM denotes /
T23894 42083-42084 NN denotes L
T23895 42085-42091 NN denotes sodium
T23896 42103-42105 , denotes ,
T23897 42105-42107 CD denotes 20
T23898 42107-42108 NN denotes %
T23899 42109-42112 NN denotes FBS
T23900 42112-42113 . denotes .
T23901 42113-42179 sentence denotes Transient transfection was carried out using TransIt LT1 (Mirus).
T23902 42114-42123 JJ denotes Transient
T23903 42124-42136 NN denotes transfection
T23904 42141-42148 VBN denotes carried
T23905 42137-42140 VBD denotes was
T23906 42149-42152 RP denotes out
T23907 42153-42158 VBG denotes using
T23908 42159-42166 NN denotes TransIt
T23909 42167-42170 NN denotes LT1
T23910 42171-42172 -LRB- denotes (
T23911 42172-42177 NNP denotes Mirus
T23912 42177-42178 -RRB- denotes )
T23913 42178-42179 . denotes .
T24169 42181-42189 NNP denotes Northern
T24170 42190-42194 NN denotes blot
T24171 42189-42190 HYPH denotes -
T24172 42195-42203 NN denotes analysis
T24173 42203-42279 sentence denotes RNA was extracted from the tissues of adult mice using Trizol (Invitrogen).
T24174 42204-42207 NN denotes RNA
T24175 42212-42221 VBN denotes extracted
T24176 42208-42211 VBD denotes was
T24177 42222-42226 IN denotes from
T24178 42227-42230 DT denotes the
T24179 42231-42238 NNS denotes tissues
T24180 42239-42241 IN denotes of
T24181 42242-42247 JJ denotes adult
T24182 42248-42252 NNS denotes mice
T24183 42253-42258 VBG denotes using
T24184 42259-42265 NNP denotes Trizol
T24185 42266-42267 -LRB- denotes (
T24186 42267-42277 NNP denotes Invitrogen
T24187 42277-42278 -RRB- denotes )
T24188 42278-42279 . denotes .
T24189 42279-42416 sentence denotes A 5 μg of total RNA was electrophoresed in a 1.0% agarose-formaldehyde gel and transferred to a nylon membrane (Zeta-Probe GT, Bio-Rad).
T24190 42280-42281 DT denotes A
T24191 42284-42286 NN denotes μg
T24192 42282-42283 CD denotes 5
T24193 42304-42319 VBN denotes electrophoresed
T24194 42287-42289 IN denotes of
T24195 42290-42295 JJ denotes total
T24196 42296-42299 NN denotes RNA
T24197 42300-42303 VBD denotes was
T24198 42320-42322 IN denotes in
T24199 42323-42324 DT denotes a
T24200 42351-42354 NN denotes gel
T24201 42325-42328 CD denotes 1.0
T24202 42328-42329 NN denotes %
T24203 42330-42337 NN denotes agarose
T24204 42338-42350 NN denotes formaldehyde
T24205 42337-42338 HYPH denotes -
T24206 42355-42358 CC denotes and
T24207 42359-42370 VBN denotes transferred
T24208 42371-42373 IN denotes to
T24209 42374-42375 DT denotes a
T24210 42382-42390 NN denotes membrane
T24211 42376-42381 NN denotes nylon
T24212 42391-42392 -LRB- denotes (
T24213 42403-42405 NNP denotes GT
T24214 42392-42396 NNP denotes Zeta
T24215 42397-42402 NNP denotes Probe
T24216 42396-42397 HYPH denotes -
T24217 42405-42407 , denotes ,
T24218 42407-42410 NNP denotes Bio
T24219 42411-42414 NNP denotes Rad
T24220 42410-42411 HYPH denotes -
T24221 42414-42415 -RRB- denotes )
T24222 42415-42416 . denotes .
T24223 42416-42512 sentence denotes The 735-bp cDNA fragment encoding the SAM domain of mr-s was used as a probe for hybridization.
T24224 42417-42420 DT denotes The
T24225 42433-42441 NN denotes fragment
T24226 42421-42424 CD denotes 735
T24227 42425-42427 NN denotes bp
T24228 42424-42425 HYPH denotes -
T24229 42428-42432 NN denotes cDNA
T24230 42478-42482 VBN denotes used
T24231 42442-42450 VBG denotes encoding
T24232 42451-42454 DT denotes the
T24233 42459-42465 NN denotes domain
T24234 42455-42458 NN denotes SAM
T24235 42466-42468 IN denotes of
T24236 42469-42471 NN denotes mr
T24237 42472-42473 NN denotes s
T24238 42471-42472 HYPH denotes -
T24239 42474-42477 VBD denotes was
T24240 42483-42485 IN denotes as
T24241 42486-42487 DT denotes a
T24242 42488-42493 NN denotes probe
T24243 42494-42497 IN denotes for
T24244 42498-42511 NN denotes hybridization
T24245 42511-42512 . denotes .
T24246 42512-42582 sentence denotes Hybridization was performed according to the manufacturer's protocol.
T24247 42513-42526 NN denotes Hybridization
T24248 42531-42540 VBN denotes performed
T24249 42527-42530 VBD denotes was
T24250 42541-42550 VBG denotes according
T24251 42551-42553 IN denotes to
T24252 42554-42557 DT denotes the
T24253 42558-42570 NN denotes manufacturer
T24254 42573-42581 NN denotes protocol
T24255 42570-42572 POS denotes 's
T24256 42581-42582 . denotes .
T24257 42582-42722 sentence denotes Washes with increasing stringency were performed, the last being at 50°C in 0.1× standard saline citrate/0.1% sodium dodecyl sulfate (SDS).
T24258 42583-42589 NNS denotes Washes
T24259 42622-42631 VBN denotes performed
T24260 42590-42594 IN denotes with
T24261 42595-42605 VBG denotes increasing
T24262 42606-42616 NN denotes stringency
T24263 42617-42621 VBD denotes were
T24264 42631-42633 , denotes ,
T24265 42633-42636 DT denotes the
T24266 42637-42641 JJ denotes last
T24267 42642-42647 VBG denotes being
T24268 42648-42650 IN denotes at
T24269 42651-42653 CD denotes 50
T24270 42653-42655 NN denotes °C
T24271 42656-42658 IN denotes in
T24272 42659-42662 CD denotes 0.1
T24273 42680-42687 NN denotes citrate
T24274 42662-42663 SYM denotes ×
T24275 42664-42672 JJ denotes standard
T24276 42673-42679 NN denotes saline
T24277 42687-42688 SYM denotes /
T24278 42691-42692 NN denotes %
T24279 42688-42691 CD denotes 0.1
T24280 42693-42699 NN denotes sodium
T24281 42708-42715 NN denotes sulfate
T24282 42700-42707 NN denotes dodecyl
T24283 42716-42717 -LRB- denotes (
T24284 42717-42720 NN denotes SDS
T24285 42720-42721 -RRB- denotes )
T24286 42721-42722 . denotes .
T24536 42724-42726 NN denotes RT
T24537 42727-42730 NN denotes PCR
T24538 42726-42727 HYPH denotes -
T24539 42731-42739 NN denotes analysis
T24540 42739-42793 sentence denotes Total RNA was isolated from each tissue using Trizol.
T24541 42740-42745 JJ denotes Total
T24542 42746-42749 NN denotes RNA
T24543 42754-42762 VBN denotes isolated
T24544 42750-42753 VBD denotes was
T24545 42763-42767 IN denotes from
T24546 42768-42772 DT denotes each
T24547 42773-42779 NN denotes tissue
T24548 42780-42785 VBG denotes using
T24549 42786-42792 NN denotes Trizol
T24550 42792-42793 . denotes .
T24551 42793-42871 sentence denotes A 1 μg of total RNA was reverse transcribed using SuperscriptII (Invitrogen).
T24552 42794-42795 DT denotes A
T24553 42798-42800 NN denotes μg
T24554 42796-42797 CD denotes 1
T24555 42826-42837 VBN denotes transcribed
T24556 42801-42803 IN denotes of
T24557 42804-42809 JJ denotes total
T24558 42810-42813 NN denotes RNA
T24559 42814-42817 VBD denotes was
T24560 42818-42825 JJ denotes reverse
T24561 42838-42843 VBG denotes using
T24562 42844-42857 NNP denotes SuperscriptII
T24563 42858-42859 -LRB- denotes (
T24564 42859-42869 NNP denotes Invitrogen
T24565 42869-42870 -RRB- denotes )
T24566 42870-42871 . denotes .
T24567 42871-43012 sentence denotes RT-PCR primers, which span introns, for detection of mr-s cDNA were 5'-TGTCCAGCCCAGCCAACCCAAGGAGACGACA-3' and 5'-TGTGGTCTCCTCATCAGTGAAGA-3'.
T24568 42872-42874 NN denotes RT
T24569 42875-42878 NN denotes PCR
T24570 42874-42875 HYPH denotes -
T24571 42879-42886 NNS denotes primers
T24572 42935-42939 VBD denotes were
T24573 42886-42888 , denotes ,
T24574 42888-42893 WDT denotes which
T24575 42894-42898 VBP denotes span
T24576 42899-42906 NNS denotes introns
T24577 42906-42908 , denotes ,
T24578 42908-42911 IN denotes for
T24579 42912-42921 NN denotes detection
T24580 42922-42924 IN denotes of
T24581 42925-42927 NN denotes mr
T24582 42928-42929 NN denotes s
T24583 42927-42928 HYPH denotes -
T24584 42930-42934 NN denotes cDNA
T24585 42940-42941 CD denotes 5
T24586 42943-42974 NN denotes TGTCCAGCCCAGCCAACCCAAGGAGACGACA
T24587 42941-42942 SYM denotes '
T24588 42942-42943 HYPH denotes -
T24589 42974-42975 HYPH denotes -
T24590 42975-42976 CD denotes 3
T24591 42976-42977 SYM denotes '
T24592 42978-42981 CC denotes and
T24593 42982-42983 CD denotes 5
T24594 42985-43008 NN denotes TGTGGTCTCCTCATCAGTGAAGA
T24595 42983-42984 SYM denotes '
T24596 42984-42985 HYPH denotes -
T24597 43008-43009 HYPH denotes -
T24598 43009-43010 CD denotes 3
T24599 43010-43011 SYM denotes '
T24600 43011-43012 . denotes .
T24601 43012-43096 sentence denotes Product size was 292 bp (positions 965–1256 of Genbank Accession Number #AY458844).
T24602 43013-43020 NN denotes Product
T24603 43021-43025 NN denotes size
T24604 43026-43029 VBD denotes was
T24605 43030-43033 CD denotes 292
T24606 43034-43036 NN denotes bp
T24607 43037-43038 -LRB- denotes (
T24608 43048-43051 CD denotes 965
T24609 43038-43047 NNS denotes positions
T24610 43051-43052 SYM denotes –
T24611 43052-43056 CD denotes 1256
T24612 43057-43059 IN denotes of
T24613 43060-43067 NNP denotes Genbank
T24614 43086-43094 NN denotes AY458844
T24615 43068-43077 NNP denotes Accession
T24616 43078-43084 NNP denotes Number
T24617 43085-43086 SYM denotes #
T24618 43094-43095 -RRB- denotes )
T24619 43095-43096 . denotes .
T24620 43096-43281 sentence denotes Primer pairs for mouse G3PDH were 5'-ACCACAGTCCATGCCATCAC-3' and 5'-TCCACCACCCTGTTGCTGTA- 3' which amplified a 452-bp product (positions 587–1038 of Genbank Accession Number #BC85275).
T24621 43097-43103 NN denotes Primer
T24622 43104-43109 NNS denotes pairs
T24623 43126-43130 VBD denotes were
T24624 43110-43113 IN denotes for
T24625 43114-43119 NN denotes mouse
T24626 43120-43125 NN denotes G3PDH
T24627 43131-43132 CD denotes 5
T24628 43134-43154 NN denotes ACCACAGTCCATGCCATCAC
T24629 43132-43133 SYM denotes '
T24630 43133-43134 HYPH denotes -
T24631 43154-43155 HYPH denotes -
T24632 43155-43156 CD denotes 3
T24633 43156-43157 SYM denotes '
T24634 43158-43161 CC denotes and
T24635 43162-43163 CD denotes 5
T24636 43165-43185 NN denotes TCCACCACCCTGTTGCTGTA
T24637 43163-43164 SYM denotes '
T24638 43164-43165 HYPH denotes -
T24639 43185-43186 HYPH denotes -
T24640 43187-43188 CD denotes 3
T24641 43188-43189 SYM denotes '
T24642 43190-43195 WDT denotes which
T24643 43196-43205 VBD denotes amplified
T24644 43206-43207 DT denotes a
T24645 43215-43222 NN denotes product
T24646 43208-43211 CD denotes 452
T24647 43212-43214 NN denotes bp
T24648 43211-43212 HYPH denotes -
T24649 43223-43224 -LRB- denotes (
T24650 43234-43237 CD denotes 587
T24651 43224-43233 NNS denotes positions
T24652 43237-43238 SYM denotes –
T24653 43238-43242 CD denotes 1038
T24654 43243-43245 IN denotes of
T24655 43246-43253 NNP denotes Genbank
T24656 43272-43279 NN denotes BC85275
T24657 43254-43263 NN denotes Accession
T24658 43264-43270 NN denotes Number
T24659 43271-43272 SYM denotes #
T24660 43279-43280 -RRB- denotes )
T24661 43280-43281 . denotes .
T25116 43283-43288 NN denotes Yeast
T25117 43300-43309 NN denotes screening
T25118 43289-43292 CD denotes two
T25119 43293-43299 NN denotes hybrid
T25120 43292-43293 HYPH denotes -
T25121 43310-43313 CC denotes and
T25122 43314-43318 NN denotes GAL4
T25123 43319-43324 NN denotes assay
T25124 43324-43466 sentence denotes We carried out yeast two-hybrid experiments using the MATCHMAKER GAL4 two-hybrid system 3 (BD Bioscience) as recommended by the manufacturer.
T25125 43325-43327 PRP denotes We
T25126 43328-43335 VBD denotes carried
T25127 43336-43339 RP denotes out
T25128 43340-43345 NN denotes yeast
T25129 43357-43368 NNS denotes experiments
T25130 43346-43349 CD denotes two
T25131 43350-43356 NN denotes hybrid
T25132 43349-43350 HYPH denotes -
T25133 43369-43374 VBG denotes using
T25134 43375-43378 DT denotes the
T25135 43406-43412 NN denotes system
T25136 43379-43389 NNP denotes MATCHMAKER
T25137 43390-43394 NN denotes GAL4
T25138 43395-43398 CD denotes two
T25139 43399-43405 NN denotes hybrid
T25140 43398-43399 HYPH denotes -
T25141 43413-43414 CD denotes 3
T25142 43415-43416 -LRB- denotes (
T25143 43419-43429 NNP denotes Bioscience
T25144 43416-43418 NNP denotes BD
T25145 43429-43430 -RRB- denotes )
T25146 43431-43433 IN denotes as
T25147 43434-43445 VBN denotes recommended
T25148 43446-43448 IN denotes by
T25149 43449-43452 DT denotes the
T25150 43453-43465 NN denotes manufacturer
T25151 43465-43466 . denotes .
T25152 43466-43603 sentence denotes We cloned the full-length mr-s into the pGBKT7 vector and used it to screen a library of mouse P0-P3 retinal cDNAs in the pGADT7 vector.
T25153 43467-43469 PRP denotes We
T25154 43470-43476 VBD denotes cloned
T25155 43477-43480 DT denotes the
T25156 43496-43497 NN denotes s
T25157 43481-43485 JJ denotes full
T25158 43486-43492 NN denotes length
T25159 43485-43486 HYPH denotes -
T25160 43493-43495 NN denotes mr
T25161 43495-43496 HYPH denotes -
T25162 43498-43502 IN denotes into
T25163 43503-43506 DT denotes the
T25164 43514-43520 NN denotes vector
T25165 43507-43513 NN denotes pGBKT7
T25166 43521-43524 CC denotes and
T25167 43525-43529 VBD denotes used
T25168 43530-43532 PRP denotes it
T25169 43533-43535 TO denotes to
T25170 43536-43542 VB denotes screen
T25171 43543-43544 DT denotes a
T25172 43545-43552 NN denotes library
T25173 43553-43555 IN denotes of
T25174 43556-43561 NN denotes mouse
T25175 43576-43581 NNS denotes cDNAs
T25176 43562-43564 NN denotes P0
T25177 43564-43565 SYM denotes -
T25178 43565-43567 NN denotes P3
T25179 43568-43575 JJ denotes retinal
T25180 43582-43584 IN denotes in
T25181 43585-43588 DT denotes the
T25182 43596-43602 NN denotes vector
T25183 43589-43595 NN denotes pGADT7
T25184 43602-43603 . denotes .
T25185 43603-43728 sentence denotes Transformants that conferred growth were picked, isolated and re-introduced with the bait into AH109 to confirm interaction.
T25186 43604-43617 NNS denotes Transformants
T25187 43645-43651 VBN denotes picked
T25188 43618-43622 WDT denotes that
T25189 43623-43632 VBD denotes conferred
T25190 43633-43639 NN denotes growth
T25191 43640-43644 VBD denotes were
T25192 43651-43653 , denotes ,
T25193 43653-43661 VBN denotes isolated
T25194 43662-43665 CC denotes and
T25195 43666-43679 VBN denotes re-introduced
T25196 43680-43684 IN denotes with
T25197 43685-43688 DT denotes the
T25198 43689-43693 NN denotes bait
T25199 43694-43698 IN denotes into
T25200 43699-43704 NN denotes AH109
T25201 43705-43707 TO denotes to
T25202 43708-43715 VB denotes confirm
T25203 43716-43727 NN denotes interaction
T25204 43727-43728 . denotes .
T25205 43728-43814 sentence denotes Plasmid DNA was isolated from yeast using RPM Yeast Plasmid Isolation Kit (Qbiogene).
T25206 43729-43736 NN denotes Plasmid
T25207 43737-43740 NN denotes DNA
T25208 43745-43753 VBN denotes isolated
T25209 43741-43744 VBD denotes was
T25210 43754-43758 IN denotes from
T25211 43759-43764 NN denotes yeast
T25212 43765-43770 VBG denotes using
T25213 43771-43774 NN denotes RPM
T25214 43799-43802 NN denotes Kit
T25215 43775-43780 NN denotes Yeast
T25216 43781-43788 NN denotes Plasmid
T25217 43789-43798 NN denotes Isolation
T25218 43803-43804 -LRB- denotes (
T25219 43804-43812 NNP denotes Qbiogene
T25220 43812-43813 -RRB- denotes )
T25221 43813-43814 . denotes .
T25222 43814-43989 sentence denotes In order to confirm the protein interactions, single colonies were picked and grown individually in synthetic complete media lacking leucine, tryptophan and containing X-gal.
T25223 43815-43817 IN denotes In
T25224 43882-43888 VBN denotes picked
T25225 43818-43823 NN denotes order
T25226 43824-43826 TO denotes to
T25227 43827-43834 VB denotes confirm
T25228 43835-43838 DT denotes the
T25229 43847-43859 NNS denotes interactions
T25230 43839-43846 NN denotes protein
T25231 43859-43861 , denotes ,
T25232 43861-43867 JJ denotes single
T25233 43868-43876 NNS denotes colonies
T25234 43877-43881 VBD denotes were
T25235 43889-43892 CC denotes and
T25236 43893-43898 VBN denotes grown
T25237 43899-43911 RB denotes individually
T25238 43912-43914 IN denotes in
T25239 43915-43924 JJ denotes synthetic
T25240 43934-43939 NNS denotes media
T25241 43925-43933 JJ denotes complete
T25242 43940-43947 VBG denotes lacking
T25243 43948-43955 NN denotes leucine
T25244 43957-43967 NN denotes tryptophan
T25245 43955-43957 , denotes ,
T25246 43968-43971 CC denotes and
T25247 43972-43982 VBG denotes containing
T25248 43983-43984 NN denotes X
T25249 43985-43988 NN denotes gal
T25250 43984-43985 HYPH denotes -
T25251 43988-43989 . denotes .
T25252 43989-44055 sentence denotes After 4–5 days, X-gal positive clones were selected and analyzed.
T25253 43990-43995 IN denotes After
T25254 44033-44041 VBN denotes selected
T25255 43996-43997 CD denotes 4
T25256 43998-43999 CD denotes 5
T25257 43997-43998 SYM denotes –
T25258 44000-44004 NNS denotes days
T25259 44004-44006 , denotes ,
T25260 44006-44007 NN denotes X
T25261 44008-44011 NN denotes gal
T25262 44007-44008 NN denotes -
T25263 44012-44020 JJ denotes positive
T25264 44021-44027 NNS denotes clones
T25265 44028-44032 VBD denotes were
T25266 44042-44045 CC denotes and
T25267 44046-44054 VBN denotes analyzed
T25268 44054-44055 . denotes .
T25269 44055-44227 sentence denotes To test self-interaction of mr-s, we subcloned the full-length, N-terminus (amino acids 1–400) and C-terminus (amino acids 391–542) of mr-s into pGBKT7 and pGADT7 vectors.
T25270 44056-44058 TO denotes To
T25271 44059-44063 VB denotes test
T25272 44093-44102 VBD denotes subcloned
T25273 44064-44068 NN denotes self
T25274 44069-44080 NN denotes interaction
T25275 44068-44069 HYPH denotes -
T25276 44081-44083 IN denotes of
T25277 44084-44086 NN denotes mr
T25278 44087-44088 NN denotes s
T25279 44086-44087 HYPH denotes -
T25280 44088-44090 , denotes ,
T25281 44090-44092 PRP denotes we
T25282 44103-44106 DT denotes the
T25283 44112-44118 NN denotes length
T25284 44107-44111 JJ denotes full
T25285 44111-44112 HYPH denotes -
T25286 44118-44120 , denotes ,
T25287 44120-44121 NN denotes N
T25288 44122-44130 NN denotes terminus
T25289 44121-44122 HYPH denotes -
T25290 44131-44132 -LRB- denotes (
T25291 44144-44145 CD denotes 1
T25292 44132-44137 NN denotes amino
T25293 44138-44143 NNS denotes acids
T25294 44145-44146 SYM denotes –
T25295 44146-44149 CD denotes 400
T25296 44149-44150 -RRB- denotes )
T25297 44151-44154 CC denotes and
T25298 44155-44156 NN denotes C
T25299 44157-44165 NN denotes terminus
T25300 44156-44157 HYPH denotes -
T25301 44166-44167 -LRB- denotes (
T25302 44179-44182 CD denotes 391
T25303 44167-44172 NN denotes amino
T25304 44173-44178 NNS denotes acids
T25305 44182-44183 SYM denotes –
T25306 44183-44186 CD denotes 542
T25307 44186-44187 -RRB- denotes )
T25308 44188-44190 IN denotes of
T25309 44191-44193 NN denotes mr
T25310 44194-44195 NN denotes s
T25311 44193-44194 HYPH denotes -
T25312 44196-44200 IN denotes into
T25313 44201-44207 NN denotes pGBKT7
T25314 44219-44226 NNS denotes vectors
T25315 44208-44211 CC denotes and
T25316 44212-44218 NN denotes pGADT7
T25317 44226-44227 . denotes .
T25318 44227-44312 sentence denotes We assessed interactions by scoring blue color on plates of medium containing X-gal.
T25319 44228-44230 PRP denotes We
T25320 44231-44239 VBD denotes assessed
T25321 44240-44252 NNS denotes interactions
T25322 44253-44255 IN denotes by
T25323 44256-44263 VBG denotes scoring
T25324 44264-44268 JJ denotes blue
T25325 44269-44274 NN denotes color
T25326 44275-44277 IN denotes on
T25327 44278-44284 NNS denotes plates
T25328 44285-44287 IN denotes of
T25329 44288-44294 NN denotes medium
T25330 44295-44305 VBG denotes containing
T25331 44306-44307 NN denotes X
T25332 44308-44311 NN denotes gal
T25333 44307-44308 HYPH denotes -
T25334 44311-44312 . denotes .
T26198 44314-44333 NN denotes Immunoprecipitation
T26199 44334-44339 NN denotes assay
T26200 44339-44665 sentence denotes For immunoprecipitation assay, 4× hemagglutinin (HA) or 3× Flag tagged cDNA fragment encoding full-length mr-s (full-HA and Flag-mrs), a cDNA fragment encoding amino acids 1–400 (ΔSAM-HA and Flag-ΔSAM), and a cDNA fragment encoding amino acids 400–542 (Flag-SAM) were subcloned into the pcDNA3 (Invitrogen) expression vector.
T26201 44340-44343 IN denotes For
T26202 44608-44617 VBN denotes subcloned
T26203 44344-44363 NN denotes immunoprecipitation
T26204 44364-44369 NN denotes assay
T26205 44369-44371 , denotes ,
T26206 44371-44372 CD denotes 4
T26207 44374-44387 NN denotes hemagglutinin
T26208 44372-44373 SYM denotes ×
T26209 44404-44410 VBN denotes tagged
T26210 44388-44389 -LRB- denotes (
T26211 44389-44391 NN denotes HA
T26212 44391-44392 -RRB- denotes )
T26213 44393-44395 CC denotes or
T26214 44396-44397 CD denotes 3
T26215 44399-44403 NN denotes Flag
T26216 44397-44398 SYM denotes ×
T26217 44416-44424 NN denotes fragment
T26218 44411-44415 NN denotes cDNA
T26219 44425-44433 VBG denotes encoding
T26220 44434-44438 JJ denotes full
T26221 44439-44445 NN denotes length
T26222 44438-44439 HYPH denotes -
T26223 44449-44450 NN denotes s
T26224 44446-44448 NN denotes mr
T26225 44448-44449 HYPH denotes -
T26226 44451-44452 -LRB- denotes (
T26227 44457-44459 NN denotes HA
T26228 44452-44456 JJ denotes full
T26229 44456-44457 HYPH denotes -
T26230 44460-44463 CC denotes and
T26231 44464-44468 NN denotes Flag
T26232 44469-44472 NN denotes mrs
T26233 44468-44469 HYPH denotes -
T26234 44472-44473 -RRB- denotes )
T26235 44473-44475 , denotes ,
T26236 44475-44476 DT denotes a
T26237 44482-44490 NN denotes fragment
T26238 44477-44481 NN denotes cDNA
T26239 44491-44499 VBG denotes encoding
T26240 44500-44505 NN denotes amino
T26241 44512-44513 CD denotes 1
T26242 44506-44511 NNS denotes acids
T26243 44513-44514 SYM denotes –
T26244 44514-44517 CD denotes 400
T26245 44518-44519 -LRB- denotes (
T26246 44524-44526 NN denotes HA
T26247 44519-44523 NN denotes ΔSAM
T26248 44523-44524 HYPH denotes -
T26249 44527-44530 CC denotes and
T26250 44531-44535 NN denotes Flag
T26251 44536-44540 NN denotes ΔSAM
T26252 44535-44536 HYPH denotes -
T26253 44540-44541 -RRB- denotes )
T26254 44541-44543 , denotes ,
T26255 44543-44546 CC denotes and
T26256 44547-44548 DT denotes a
T26257 44554-44562 NN denotes fragment
T26258 44549-44553 NN denotes cDNA
T26259 44563-44571 VBG denotes encoding
T26260 44572-44577 NN denotes amino
T26261 44584-44587 CD denotes 400
T26262 44578-44583 NNS denotes acids
T26263 44587-44588 SYM denotes –
T26264 44588-44591 CD denotes 542
T26265 44592-44593 -LRB- denotes (
T26266 44598-44601 NN denotes SAM
T26267 44593-44597 NN denotes Flag
T26268 44597-44598 HYPH denotes -
T26269 44601-44602 -RRB- denotes )
T26270 44603-44607 VBD denotes were
T26271 44618-44622 IN denotes into
T26272 44623-44626 DT denotes the
T26273 44658-44664 NN denotes vector
T26274 44627-44633 NN denotes pcDNA3
T26275 44634-44635 -LRB- denotes (
T26276 44635-44645 NNP denotes Invitrogen
T26277 44645-44646 -RRB- denotes )
T26278 44647-44657 NN denotes expression
T26279 44664-44665 . denotes .
T26280 44665-44750 sentence denotes We also constructed two site-directed mutants, Flag-W404A and Flag-G453A, using PCR.
T26281 44666-44668 PRP denotes We
T26282 44674-44685 VBD denotes constructed
T26283 44669-44673 RB denotes also
T26284 44686-44689 CD denotes two
T26285 44704-44711 NNS denotes mutants
T26286 44690-44694 NN denotes site
T26287 44695-44703 VBN denotes directed
T26288 44694-44695 HYPH denotes -
T26289 44711-44713 , denotes ,
T26290 44713-44717 NN denotes Flag
T26291 44718-44723 NN denotes W404A
T26292 44717-44718 HYPH denotes -
T26293 44724-44727 CC denotes and
T26294 44728-44732 NN denotes Flag
T26295 44733-44738 NN denotes G453A
T26296 44732-44733 HYPH denotes -
T26297 44738-44740 , denotes ,
T26298 44740-44745 VBG denotes using
T26299 44746-44749 NN denotes PCR
T26300 44749-44750 . denotes .
T26301 44750-44810 sentence denotes Each of these mutations was also introduced into Flag-mr-s.
T26302 44751-44755 DT denotes Each
T26303 44784-44794 VBN denotes introduced
T26304 44756-44758 IN denotes of
T26305 44759-44764 DT denotes these
T26306 44765-44774 NNS denotes mutations
T26307 44775-44778 VBD denotes was
T26308 44779-44783 RB denotes also
T26309 44795-44799 IN denotes into
T26310 44800-44804 NN denotes Flag
T26311 44804-44805 HYPH denotes -
T26312 44805-44807 NN denotes mr
T26313 44808-44809 NN denotes s
T26314 44807-44808 HYPH denotes -
T26315 44809-44810 . denotes .
T26316 44810-44907 sentence denotes We transfected HEK293T cells with 5 μg of plasmid DNA per 6 cm dish by calcium phosphate method.
T26317 44811-44813 PRP denotes We
T26318 44814-44825 VBD denotes transfected
T26319 44826-44833 NN denotes HEK293T
T26320 44834-44839 NNS denotes cells
T26321 44840-44844 IN denotes with
T26322 44845-44846 CD denotes 5
T26323 44847-44849 NN denotes μg
T26324 44850-44852 IN denotes of
T26325 44853-44860 NN denotes plasmid
T26326 44861-44864 NN denotes DNA
T26327 44865-44868 IN denotes per
T26328 44869-44870 CD denotes 6
T26329 44871-44873 NN denotes cm
T26330 44874-44878 NN denotes dish
T26331 44879-44881 IN denotes by
T26332 44882-44889 NN denotes calcium
T26333 44890-44899 NN denotes phosphate
T26334 44900-44906 NN denotes method
T26335 44906-44907 . denotes .
T26336 44907-45198 sentence denotes Approximately 48 hr after transfection, cells were harvested in immunoprecipitation buffer (50 mM Tris-HCl [pH 7.5], 1 mM EDTA, 2 mM MgCl2, 150 mM NaCl, 1 mM phenylmethylsulfonyl fluoride [Wako], 1% Nonidet P-40, 10% glycerol) in the presence of protease inhibitor cocktail tablets (Roche).
T26337 44908-44921 RB denotes Approximately
T26338 44922-44924 CD denotes 48
T26339 44925-44927 NN denotes hr
T26340 44928-44933 IN denotes after
T26341 44959-44968 VBN denotes harvested
T26342 44934-44946 NN denotes transfection
T26343 44946-44948 , denotes ,
T26344 44948-44953 NNS denotes cells
T26345 44954-44958 VBD denotes were
T26346 44969-44971 IN denotes in
T26347 44972-44991 NN denotes immunoprecipitation
T26348 44992-44998 NN denotes buffer
T26349 44999-45000 -LRB- denotes (
T26350 45011-45014 NN denotes HCl
T26351 45000-45002 CD denotes 50
T26352 45003-45005 NN denotes mM
T26353 45006-45010 NN denotes Tris
T26354 45010-45011 HYPH denotes -
T26355 45015-45016 -LRB- denotes [
T26356 45016-45018 NN denotes pH
T26357 45019-45022 CD denotes 7.5
T26358 45022-45023 -RRB- denotes ]
T26359 45023-45025 , denotes ,
T26360 45025-45026 CD denotes 1
T26361 45027-45029 NN denotes mM
T26362 45030-45034 NN denotes EDTA
T26363 45034-45036 , denotes ,
T26364 45036-45037 CD denotes 2
T26365 45038-45040 NN denotes mM
T26366 45041-45046 NN denotes MgCl2
T26367 45046-45048 , denotes ,
T26368 45048-45051 CD denotes 150
T26369 45052-45054 NN denotes mM
T26370 45055-45059 NN denotes NaCl
T26371 45059-45061 , denotes ,
T26372 45061-45062 CD denotes 1
T26373 45063-45065 NN denotes mM
T26374 45087-45095 NN denotes fluoride
T26375 45066-45086 NN denotes phenylmethylsulfonyl
T26376 45096-45097 -LRB- denotes [
T26377 45097-45101 NN denotes Wako
T26378 45101-45102 -RRB- denotes ]
T26379 45102-45104 , denotes ,
T26380 45104-45105 CD denotes 1
T26381 45105-45106 NN denotes %
T26382 45115-45116 NN denotes P
T26383 45107-45114 NN denotes Nonidet
T26384 45116-45117 HYPH denotes -
T26385 45117-45119 CD denotes 40
T26386 45119-45121 , denotes ,
T26387 45121-45123 CD denotes 10
T26388 45123-45124 NN denotes %
T26389 45125-45133 NN denotes glycerol
T26390 45133-45134 -RRB- denotes )
T26391 45135-45137 IN denotes in
T26392 45138-45141 DT denotes the
T26393 45142-45150 NN denotes presence
T26394 45151-45153 IN denotes of
T26395 45154-45162 NN denotes protease
T26396 45163-45172 NN denotes inhibitor
T26397 45182-45189 NNS denotes tablets
T26398 45173-45181 NN denotes cocktail
T26399 45190-45191 -LRB- denotes (
T26400 45191-45196 NNP denotes Roche
T26401 45196-45197 -RRB- denotes )
T26402 45197-45198 . denotes .
T26403 45198-45378 sentence denotes For each reaction, 1 mg of cell lysate was mixed with 0.5 μg of anti-Flag antibody (SIGMA, F3165) and 15 μl of protein G-Sepharose (Amersham) on a rotating wheel at 4°C for 2 hrs.
T26404 45199-45202 IN denotes For
T26405 45242-45247 VBN denotes mixed
T26406 45203-45207 DT denotes each
T26407 45208-45216 NN denotes reaction
T26408 45216-45218 , denotes ,
T26409 45218-45219 CD denotes 1
T26410 45220-45222 NN denotes mg
T26411 45223-45225 IN denotes of
T26412 45226-45230 NN denotes cell
T26413 45231-45237 NN denotes lysate
T26414 45238-45241 VBD denotes was
T26415 45248-45252 IN denotes with
T26416 45253-45256 CD denotes 0.5
T26417 45257-45259 NN denotes μg
T26418 45260-45262 IN denotes of
T26419 45263-45272 JJ denotes anti-Flag
T26420 45273-45281 NN denotes antibody
T26421 45282-45283 -LRB- denotes (
T26422 45290-45295 NN denotes F3165
T26423 45283-45288 NNP denotes SIGMA
T26424 45288-45290 , denotes ,
T26425 45295-45296 -RRB- denotes )
T26426 45297-45300 CC denotes and
T26427 45301-45303 CD denotes 15
T26428 45304-45306 NN denotes μl
T26429 45307-45309 IN denotes of
T26430 45310-45317 NN denotes protein
T26431 45320-45329 NN denotes Sepharose
T26432 45318-45319 NN denotes G
T26433 45319-45320 HYPH denotes -
T26434 45330-45331 -LRB- denotes (
T26435 45331-45339 NNP denotes Amersham
T26436 45339-45340 -RRB- denotes )
T26437 45341-45343 IN denotes on
T26438 45344-45345 DT denotes a
T26439 45355-45360 NN denotes wheel
T26440 45346-45354 VBG denotes rotating
T26441 45361-45363 IN denotes at
T26442 45364-45365 CD denotes 4
T26443 45365-45367 NN denotes °C
T26444 45368-45371 IN denotes for
T26445 45372-45373 CD denotes 2
T26446 45374-45377 NNS denotes hrs
T26447 45377-45378 . denotes .
T26448 45378-45457 sentence denotes Protein concentration was determined by the BCA protein assay system (Pierce).
T26449 45379-45386 NN denotes Protein
T26450 45387-45400 NN denotes concentration
T26451 45405-45415 VBN denotes determined
T26452 45401-45404 VBD denotes was
T26453 45416-45418 IN denotes by
T26454 45419-45422 DT denotes the
T26455 45441-45447 NN denotes system
T26456 45423-45426 NN denotes BCA
T26457 45435-45440 NN denotes assay
T26458 45427-45434 NN denotes protein
T26459 45448-45449 -LRB- denotes (
T26460 45449-45455 NNP denotes Pierce
T26461 45455-45456 -RRB- denotes )
T26462 45456-45457 . denotes .
T26463 45457-45626 sentence denotes The beads were then washed three times with immunoprecipitation buffer and followed by three times with wash buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 20 mM MgCl2).
T26464 45458-45461 DT denotes The
T26465 45462-45467 NNS denotes beads
T26466 45478-45484 VBN denotes washed
T26467 45468-45472 VBD denotes were
T26468 45473-45477 RB denotes then
T26469 45485-45490 CD denotes three
T26470 45491-45496 NNS denotes times
T26471 45497-45501 IN denotes with
T26472 45502-45521 NN denotes immunoprecipitation
T26473 45522-45528 NN denotes buffer
T26474 45529-45532 CC denotes and
T26475 45533-45541 VBN denotes followed
T26476 45542-45544 IN denotes by
T26477 45545-45550 CD denotes three
T26478 45551-45556 NNS denotes times
T26479 45557-45561 IN denotes with
T26480 45562-45566 NN denotes wash
T26481 45567-45573 NN denotes buffer
T26482 45574-45575 -LRB- denotes (
T26483 45586-45589 NN denotes HCl
T26484 45575-45577 CD denotes 50
T26485 45578-45580 NN denotes mM
T26486 45581-45585 NN denotes Tris
T26487 45585-45586 HYPH denotes -
T26488 45590-45591 -LRB- denotes [
T26489 45591-45593 NN denotes pH
T26490 45594-45597 CD denotes 7.5
T26491 45597-45598 -RRB- denotes ]
T26492 45598-45600 , denotes ,
T26493 45600-45603 CD denotes 150
T26494 45604-45606 NN denotes mM
T26495 45607-45611 NN denotes NaCl
T26496 45611-45613 , denotes ,
T26497 45613-45615 CD denotes 20
T26498 45616-45618 NN denotes mM
T26499 45619-45624 NN denotes MgCl2
T26500 45624-45625 -RRB- denotes )
T26501 45625-45626 . denotes .
T26502 45626-45755 sentence denotes Proteins were then boiled for 5 min in SDS sample buffer (1% SDS, 1 mM Tris [pH 6.8], 40 mM DTT, 4% glycerol, 0.01% pyronine Y).
T26503 45627-45635 NNS denotes Proteins
T26504 45646-45652 VBN denotes boiled
T26505 45636-45640 VBD denotes were
T26506 45641-45645 RB denotes then
T26507 45653-45656 IN denotes for
T26508 45657-45658 CD denotes 5
T26509 45659-45662 NN denotes min
T26510 45663-45665 IN denotes in
T26511 45666-45669 NN denotes SDS
T26512 45677-45683 NN denotes buffer
T26513 45670-45676 NN denotes sample
T26514 45684-45685 -LRB- denotes (
T26515 45688-45691 NN denotes SDS
T26516 45685-45686 CD denotes 1
T26517 45686-45687 NN denotes %
T26518 45691-45693 , denotes ,
T26519 45693-45694 CD denotes 1
T26520 45695-45697 NN denotes mM
T26521 45698-45702 NN denotes Tris
T26522 45703-45704 -LRB- denotes [
T26523 45704-45706 NN denotes pH
T26524 45707-45710 CD denotes 6.8
T26525 45710-45711 -RRB- denotes ]
T26526 45711-45713 , denotes ,
T26527 45713-45715 CD denotes 40
T26528 45716-45718 NN denotes mM
T26529 45719-45722 NN denotes DTT
T26530 45722-45724 , denotes ,
T26531 45724-45725 CD denotes 4
T26532 45725-45726 NN denotes %
T26533 45727-45735 NN denotes glycerol
T26534 45735-45737 , denotes ,
T26535 45737-45741 CD denotes 0.01
T26536 45741-45742 NN denotes %
T26537 45752-45753 NN denotes Y
T26538 45743-45751 NN denotes pyronine
T26539 45753-45754 -RRB- denotes )
T26540 45754-45755 . denotes .
T26541 45755-45948 sentence denotes The supernatants were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to the nitrocellulose membrane (Trans-Blot Transfer Medium, Bio-Rad).
T26542 45756-45759 DT denotes The
T26543 45760-45772 NNS denotes supernatants
T26544 45778-45790 VBN denotes fractionated
T26545 45773-45777 VBD denotes were
T26546 45791-45793 IN denotes by
T26547 45794-45800 NN denotes sodium
T26548 45809-45816 NN denotes sulfate
T26549 45801-45808 NN denotes dodecyl
T26550 45832-45835 NN denotes gel
T26551 45816-45817 HYPH denotes -
T26552 45817-45831 NN denotes polyacrylamide
T26553 45836-45851 NN denotes electrophoresis
T26554 45852-45853 -LRB- denotes (
T26555 45857-45861 NN denotes PAGE
T26556 45853-45856 NN denotes SDS
T26557 45856-45857 HYPH denotes -
T26558 45861-45862 -RRB- denotes )
T26559 45863-45866 CC denotes and
T26560 45867-45878 VBN denotes transferred
T26561 45879-45881 IN denotes to
T26562 45882-45885 DT denotes the
T26563 45901-45909 NN denotes membrane
T26564 45886-45900 NN denotes nitrocellulose
T26565 45910-45911 -LRB- denotes (
T26566 45931-45937 NN denotes Medium
T26567 45911-45916 NN denotes Trans
T26568 45917-45921 NN denotes Blot
T26569 45916-45917 HYPH denotes -
T26570 45922-45930 NN denotes Transfer
T26571 45937-45939 , denotes ,
T26572 45939-45942 NNP denotes Bio
T26573 45943-45946 NNP denotes Rad
T26574 45942-45943 HYPH denotes -
T26575 45946-45947 -RRB- denotes )
T26576 45947-45948 . denotes .
T26577 45948-46042 sentence denotes Western blotting was performed with rabbit polyclonal anti-HA antibody (Santa Cruz, #sc-805).
T26578 45949-45956 NNP denotes Western
T26579 45957-45965 NN denotes blotting
T26580 45970-45979 VBN denotes performed
T26581 45966-45969 VBD denotes was
T26582 45980-45984 IN denotes with
T26583 45985-45991 NN denotes rabbit
T26584 46011-46019 NN denotes antibody
T26585 45992-46002 JJ denotes polyclonal
T26586 46003-46010 JJ denotes anti-HA
T26587 46020-46021 -LRB- denotes (
T26588 46027-46031 NNP denotes Cruz
T26589 46021-46026 NNP denotes Santa
T26590 46031-46033 , denotes ,
T26591 46033-46034 SYM denotes #
T26592 46034-46036 NN denotes sc
T26593 46036-46037 HYPH denotes -
T26594 46037-46040 CD denotes 805
T26595 46040-46041 -RRB- denotes )
T26596 46041-46042 . denotes .
T26597 46042-46184 sentence denotes Signals were detected with horseradish peroxidase- conjugated goat anti-rabbit IgG and ECL plus Western Blotting Detection System (Amersham).
T26598 46043-46050 NNS denotes Signals
T26599 46056-46064 VBN denotes detected
T26600 46051-46055 VBD denotes were
T26601 46065-46069 IN denotes with
T26602 46070-46081 NN denotes horseradish
T26603 46082-46092 NN denotes peroxidase
T26604 46094-46104 VBN denotes conjugated
T26605 46092-46093 HYPH denotes -
T26606 46122-46125 NN denotes IgG
T26607 46105-46109 NN denotes goat
T26608 46110-46121 JJ denotes anti-rabbit
T26609 46126-46129 CC denotes and
T26610 46130-46133 NN denotes ECL
T26611 46166-46172 NN denotes System
T26612 46134-46138 CC denotes plus
T26613 46139-46146 NNP denotes Western
T26614 46147-46155 NN denotes Blotting
T26615 46156-46165 NN denotes Detection
T26616 46173-46174 -LRB- denotes (
T26617 46174-46182 NNP denotes Amersham
T26618 46182-46183 -RRB- denotes )
T26619 46183-46184 . denotes .
T27028 46186-46197 JJ denotes Subcellular
T27029 46211-46219 NN denotes analysis
T27030 46198-46210 NN denotes localization
T27031 46219-46495 sentence denotes We transfected the plasmid encoding HA-tagged full-length mr-s into HEK293T cells, seeded cells on coverslips coated with collagen 48 hr after transfection, fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) at room temperature for 20 min, and washed with PBS.
T27032 46220-46222 PRP denotes We
T27033 46223-46234 VBD denotes transfected
T27034 46235-46238 DT denotes the
T27035 46239-46246 NN denotes plasmid
T27036 46247-46255 VBG denotes encoding
T27037 46256-46258 NN denotes HA
T27038 46259-46265 VBN denotes tagged
T27039 46258-46259 HYPH denotes -
T27040 46281-46282 NN denotes s
T27041 46266-46270 JJ denotes full
T27042 46271-46277 NN denotes length
T27043 46270-46271 HYPH denotes -
T27044 46278-46280 NN denotes mr
T27045 46280-46281 HYPH denotes -
T27046 46283-46287 IN denotes into
T27047 46288-46295 NN denotes HEK293T
T27048 46296-46301 NNS denotes cells
T27049 46301-46303 , denotes ,
T27050 46303-46309 VBD denotes seeded
T27051 46310-46315 NNS denotes cells
T27052 46316-46318 IN denotes on
T27053 46319-46329 NNS denotes coverslips
T27054 46330-46336 VBN denotes coated
T27055 46337-46341 IN denotes with
T27056 46342-46350 NN denotes collagen
T27057 46351-46353 CD denotes 48
T27058 46354-46356 NN denotes hr
T27059 46357-46362 IN denotes after
T27060 46363-46375 NN denotes transfection
T27061 46375-46377 , denotes ,
T27062 46377-46382 VBN denotes fixed
T27063 46383-46387 IN denotes with
T27064 46388-46389 CD denotes 4
T27065 46389-46390 NN denotes %
T27066 46391-46407 NN denotes paraformaldehyde
T27067 46408-46410 IN denotes in
T27068 46411-46420 NN denotes phosphate
T27069 46421-46429 VBN denotes buffered
T27070 46420-46421 HYPH denotes -
T27071 46430-46436 NN denotes saline
T27072 46437-46438 -LRB- denotes (
T27073 46438-46441 NN denotes PBS
T27074 46441-46442 -RRB- denotes )
T27075 46443-46445 IN denotes at
T27076 46446-46450 NN denotes room
T27077 46451-46462 NN denotes temperature
T27078 46463-46466 IN denotes for
T27079 46467-46469 CD denotes 20
T27080 46470-46473 NN denotes min
T27081 46473-46475 , denotes ,
T27082 46475-46478 CC denotes and
T27083 46479-46485 VBN denotes washed
T27084 46486-46490 IN denotes with
T27085 46491-46494 NN denotes PBS
T27086 46494-46495 . denotes .
T27087 46495-46690 sentence denotes Then we permeabilized cells in PBS containing 0.1% Triton X-100 for 15 min, washed again with PBS, and incubated in PBS containing 0.1% bovine serum albumin (BSA) for 30 min at room temperature.
T27088 46496-46500 RB denotes Then
T27089 46504-46517 VBD denotes permeabilized
T27090 46501-46503 PRP denotes we
T27091 46518-46523 NNS denotes cells
T27092 46524-46526 IN denotes in
T27093 46527-46530 NN denotes PBS
T27094 46531-46541 VBG denotes containing
T27095 46542-46545 CD denotes 0.1
T27096 46545-46546 NN denotes %
T27097 46556-46559 NN denotes 100
T27098 46547-46553 NN denotes Triton
T27099 46554-46555 NN denotes X
T27100 46555-46556 HYPH denotes -
T27101 46560-46563 IN denotes for
T27102 46564-46566 CD denotes 15
T27103 46567-46570 NN denotes min
T27104 46570-46572 , denotes ,
T27105 46572-46578 VBD denotes washed
T27106 46579-46584 RB denotes again
T27107 46585-46589 IN denotes with
T27108 46590-46593 NN denotes PBS
T27109 46593-46595 , denotes ,
T27110 46595-46598 CC denotes and
T27111 46599-46608 VBD denotes incubated
T27112 46609-46611 IN denotes in
T27113 46612-46615 NN denotes PBS
T27114 46616-46626 VBG denotes containing
T27115 46627-46630 CD denotes 0.1
T27116 46630-46631 NN denotes %
T27117 46632-46638 JJ denotes bovine
T27118 46645-46652 NN denotes albumin
T27119 46639-46644 NN denotes serum
T27120 46653-46654 -LRB- denotes (
T27121 46654-46657 NN denotes BSA
T27122 46657-46658 -RRB- denotes )
T27123 46659-46662 IN denotes for
T27124 46663-46665 CD denotes 30
T27125 46666-46669 NN denotes min
T27126 46670-46672 IN denotes at
T27127 46673-46677 NN denotes room
T27128 46678-46689 NN denotes temperature
T27129 46689-46690 . denotes .
T27130 46690-46784 sentence denotes We incubated cells with anti-HA antibody overnight at 4°C (1:200 in PBS containing 0.2% BSA).
T27131 46691-46693 PRP denotes We
T27132 46694-46703 VBD denotes incubated
T27133 46704-46709 NNS denotes cells
T27134 46710-46714 IN denotes with
T27135 46715-46722 JJ denotes anti-HA
T27136 46723-46731 NN denotes antibody
T27137 46732-46741 RB denotes overnight
T27138 46742-46744 IN denotes at
T27139 46745-46746 CD denotes 4
T27140 46746-46748 NN denotes °C
T27141 46749-46750 -LRB- denotes (
T27142 46750-46751 CD denotes 1
T27143 46751-46752 SYM denotes :
T27144 46752-46755 CD denotes 200
T27145 46756-46758 IN denotes in
T27146 46759-46762 NN denotes PBS
T27147 46763-46773 VBG denotes containing
T27148 46774-46777 CD denotes 0.2
T27149 46777-46778 NN denotes %
T27150 46779-46782 NN denotes BSA
T27151 46782-46783 -RRB- denotes )
T27152 46783-46784 . denotes .
T27153 46784-46967 sentence denotes The following day, we washed cells three times with PBS and incubated with a Cy3-conjugated goat IgG against rabbit IgG (1:400 in PBS, Jackson ImmunoReseach Laboratories) for 30 min.
T27154 46785-46788 DT denotes The
T27155 46799-46802 NN denotes day
T27156 46789-46798 VBG denotes following
T27157 46807-46813 VBD denotes washed
T27158 46802-46804 , denotes ,
T27159 46804-46806 PRP denotes we
T27160 46814-46819 NNS denotes cells
T27161 46820-46825 CD denotes three
T27162 46826-46831 NNS denotes times
T27163 46832-46836 IN denotes with
T27164 46837-46840 NN denotes PBS
T27165 46841-46844 CC denotes and
T27166 46845-46854 VBD denotes incubated
T27167 46855-46859 IN denotes with
T27168 46860-46861 DT denotes a
T27169 46882-46885 NN denotes IgG
T27170 46862-46865 NN denotes Cy3
T27171 46866-46876 VBN denotes conjugated
T27172 46865-46866 HYPH denotes -
T27173 46877-46881 NN denotes goat
T27174 46886-46893 IN denotes against
T27175 46894-46900 NN denotes rabbit
T27176 46901-46904 NN denotes IgG
T27177 46905-46906 -LRB- denotes (
T27178 46942-46954 NNP denotes Laboratories
T27179 46906-46907 CD denotes 1
T27180 46907-46908 SYM denotes :
T27181 46908-46911 CD denotes 400
T27182 46912-46914 IN denotes in
T27183 46915-46918 NN denotes PBS
T27184 46918-46920 , denotes ,
T27185 46920-46927 NNP denotes Jackson
T27186 46928-46941 NNP denotes ImmunoReseach
T27187 46954-46955 -RRB- denotes )
T27188 46956-46959 IN denotes for
T27189 46960-46962 CD denotes 30
T27190 46963-46966 NN denotes min
T27191 46966-46967 . denotes .
T27192 46967-47109 sentence denotes We rinsed cells three times with PBS, followed by observation using a confocal microscope FV300 (Olympus) equipped with a 60× objective lens.
T27193 46968-46970 PRP denotes We
T27194 46971-46977 VBD denotes rinsed
T27195 46978-46983 NNS denotes cells
T27196 46984-46989 CD denotes three
T27197 46990-46995 NNS denotes times
T27198 46996-47000 IN denotes with
T27199 47001-47004 NN denotes PBS
T27200 47004-47006 , denotes ,
T27201 47006-47014 VBN denotes followed
T27202 47015-47017 IN denotes by
T27203 47018-47029 NN denotes observation
T27204 47030-47035 VBG denotes using
T27205 47036-47037 DT denotes a
T27206 47058-47063 NN denotes FV300
T27207 47038-47046 JJ denotes confocal
T27208 47047-47057 NN denotes microscope
T27209 47064-47065 -LRB- denotes (
T27210 47065-47072 NNP denotes Olympus
T27211 47072-47073 -RRB- denotes )
T27212 47074-47082 VBN denotes equipped
T27213 47083-47087 IN denotes with
T27214 47088-47089 DT denotes a
T27215 47104-47108 NN denotes lens
T27216 47090-47092 CD denotes 60
T27217 47092-47093 SYM denotes ×
T27218 47094-47103 NN denotes objective
T27219 47108-47109 . denotes .
T27909 47111-47121 NN denotes Luciferase
T27910 47122-47127 NN denotes assay
T27911 47127-47352 sentence denotes For transcriptional analysis of the 1.2-kb promoter region of mr-s, we constructed a reporter plasmid (pro1.2k) by subcloning a 1.2-kb upstream genomic fragment of mr-s gene into a pGL3 luciferase reporter plasmid (Promega).
T27912 47128-47131 IN denotes For
T27913 47199-47210 VBD denotes constructed
T27914 47132-47147 JJ denotes transcriptional
T27915 47148-47156 NN denotes analysis
T27916 47157-47159 IN denotes of
T27917 47160-47163 DT denotes the
T27918 47180-47186 NN denotes region
T27919 47164-47167 CD denotes 1.2
T27920 47168-47170 NN denotes kb
T27921 47167-47168 HYPH denotes -
T27922 47171-47179 NN denotes promoter
T27923 47187-47189 IN denotes of
T27924 47190-47192 NN denotes mr
T27925 47193-47194 NN denotes s
T27926 47192-47193 HYPH denotes -
T27927 47194-47196 , denotes ,
T27928 47196-47198 PRP denotes we
T27929 47211-47212 DT denotes a
T27930 47222-47229 NN denotes plasmid
T27931 47213-47221 NN denotes reporter
T27932 47230-47231 -LRB- denotes (
T27933 47231-47238 NN denotes pro1.2k
T27934 47238-47239 -RRB- denotes )
T27935 47240-47242 IN denotes by
T27936 47243-47253 VBG denotes subcloning
T27937 47254-47255 DT denotes a
T27938 47280-47288 NN denotes fragment
T27939 47256-47259 CD denotes 1.2
T27940 47260-47262 NN denotes kb
T27941 47259-47260 HYPH denotes -
T27942 47263-47271 JJ denotes upstream
T27943 47272-47279 JJ denotes genomic
T27944 47289-47291 IN denotes of
T27945 47292-47294 NN denotes mr
T27946 47295-47296 NN denotes s
T27947 47294-47295 HYPH denotes -
T27948 47297-47301 NN denotes gene
T27949 47302-47306 IN denotes into
T27950 47307-47308 DT denotes a
T27951 47334-47341 NN denotes plasmid
T27952 47309-47313 NN denotes pGL3
T27953 47325-47333 NN denotes reporter
T27954 47314-47324 NN denotes luciferase
T27955 47342-47343 -LRB- denotes (
T27956 47343-47350 NNP denotes Promega
T27957 47350-47351 -RRB- denotes )
T27958 47351-47352 . denotes .
T27959 47352-47514 sentence denotes The expression vectors were constructed by subcloning full-length Crx, Otx2, Nrl genes into a pMIK expression vector (a gift from Dr. K. Maruyama), respectively.
T27960 47353-47356 DT denotes The
T27961 47368-47375 NNS denotes vectors
T27962 47357-47367 NN denotes expression
T27963 47381-47392 VBN denotes constructed
T27964 47376-47380 VBD denotes were
T27965 47393-47395 IN denotes by
T27966 47396-47406 VBG denotes subcloning
T27967 47407-47411 JJ denotes full
T27968 47412-47418 NN denotes length
T27969 47411-47412 HYPH denotes -
T27970 47434-47439 NNS denotes genes
T27971 47419-47422 NN denotes Crx
T27972 47430-47433 NN denotes Nrl
T27973 47422-47424 , denotes ,
T27974 47424-47428 NN denotes Otx2
T27975 47428-47430 , denotes ,
T27976 47440-47444 IN denotes into
T27977 47445-47446 DT denotes a
T27978 47463-47469 NN denotes vector
T27979 47447-47451 NN denotes pMIK
T27980 47452-47462 NN denotes expression
T27981 47470-47471 -LRB- denotes (
T27982 47473-47477 NN denotes gift
T27983 47471-47472 DT denotes a
T27984 47478-47482 IN denotes from
T27985 47483-47486 NNP denotes Dr.
T27986 47490-47498 NNP denotes Maruyama
T27987 47487-47489 NNP denotes K.
T27988 47498-47499 -RRB- denotes )
T27989 47499-47501 , denotes ,
T27990 47501-47513 RB denotes respectively
T27991 47513-47514 . denotes .
T27992 47514-47631 sentence denotes For the "mut1259" vector, the Crx binding site at the -1259 bp position was mutated by replacing GGATTA with AGATCT.
T27993 47515-47518 IN denotes For
T27994 47591-47598 VBN denotes mutated
T27995 47519-47522 DT denotes the
T27996 47533-47539 NN denotes vector
T27997 47523-47524 `` denotes "
T27998 47524-47531 NN denotes mut1259
T27999 47531-47532 '' denotes "
T28000 47539-47541 , denotes ,
T28001 47541-47544 DT denotes the
T28002 47557-47561 NN denotes site
T28003 47545-47548 NN denotes Crx
T28004 47549-47556 NN denotes binding
T28005 47562-47564 IN denotes at
T28006 47565-47568 DT denotes the
T28007 47578-47586 NN denotes position
T28008 47569-47570 SYM denotes -
T28009 47570-47574 CD denotes 1259
T28010 47575-47577 NN denotes bp
T28011 47587-47590 VBD denotes was
T28012 47599-47601 IN denotes by
T28013 47602-47611 VBG denotes replacing
T28014 47612-47618 NN denotes GGATTA
T28015 47619-47623 IN denotes with
T28016 47624-47630 NN denotes AGATCT
T28017 47630-47631 . denotes .
T28018 47631-47746 sentence denotes For the "mut198" vector, the Crx binding site at the -198 bp position was mutated by replacing TAATCC with GAATTC.
T28019 47632-47635 IN denotes For
T28020 47706-47713 VBN denotes mutated
T28021 47636-47639 DT denotes the
T28022 47649-47655 NN denotes vector
T28023 47640-47641 `` denotes "
T28024 47641-47647 NN denotes mut198
T28025 47647-47648 '' denotes "
T28026 47655-47657 , denotes ,
T28027 47657-47660 DT denotes the
T28028 47673-47677 NN denotes site
T28029 47661-47664 NN denotes Crx
T28030 47665-47672 NN denotes binding
T28031 47678-47680 IN denotes at
T28032 47681-47684 DT denotes the
T28033 47693-47701 NN denotes position
T28034 47685-47686 SYM denotes -
T28035 47686-47689 CD denotes 198
T28036 47690-47692 NN denotes bp
T28037 47702-47705 VBD denotes was
T28038 47714-47716 IN denotes by
T28039 47717-47726 VBG denotes replacing
T28040 47727-47733 NN denotes TAATCC
T28041 47734-47738 IN denotes with
T28042 47739-47745 NN denotes GAATTC
T28043 47745-47746 . denotes .
T28044 47746-47859 sentence denotes For the "mut72" vector, the Crx binding site at the -72 bp position was mutated by replacing GGATTA with GAATTC.
T28045 47747-47750 IN denotes For
T28046 47819-47826 VBN denotes mutated
T28047 47751-47754 DT denotes the
T28048 47763-47769 NN denotes vector
T28049 47755-47756 `` denotes "
T28050 47756-47761 NN denotes mut72
T28051 47761-47762 '' denotes "
T28052 47769-47771 , denotes ,
T28053 47771-47774 DT denotes the
T28054 47787-47791 NN denotes site
T28055 47775-47778 NN denotes Crx
T28056 47779-47786 NN denotes binding
T28057 47792-47794 IN denotes at
T28058 47795-47798 DT denotes the
T28059 47806-47814 NN denotes position
T28060 47799-47800 SYM denotes -
T28061 47800-47802 CD denotes 72
T28062 47803-47805 NN denotes bp
T28063 47815-47818 VBD denotes was
T28064 47827-47829 IN denotes by
T28065 47830-47839 VBG denotes replacing
T28066 47840-47846 NN denotes GGATTA
T28067 47847-47851 IN denotes with
T28068 47852-47858 NN denotes GAATTC
T28069 47858-47859 . denotes .
T28070 47859-47930 sentence denotes For the "mut all" vector, all of three Crx binding sites were mutated.
T28071 47860-47863 IN denotes For
T28072 47922-47929 VBN denotes mutated
T28073 47864-47867 DT denotes the
T28074 47878-47884 NN denotes vector
T28075 47868-47869 `` denotes "
T28076 47869-47872 NN denotes mut
T28077 47873-47876 NN denotes all
T28078 47876-47877 '' denotes "
T28079 47884-47886 , denotes ,
T28080 47886-47889 DT denotes all
T28081 47890-47892 IN denotes of
T28082 47893-47898 CD denotes three
T28083 47911-47916 NNS denotes sites
T28084 47899-47902 NN denotes Crx
T28085 47903-47910 NN denotes binding
T28086 47917-47921 VBD denotes were
T28087 47929-47930 . denotes .
T28088 47930-48196 sentence denotes 5xGAL4-pGL3 Control reporter plasmid, which contains five copies of the GAL4 DNA recognition sequence positioned immediately upstream of SV40 minimal promoter, was kindly gifted from Dr. T. Noguchi and used for analysis of the transcriptional activity of mr-s [50].
T28089 47931-47937 NN denotes 5xGAL4
T28090 47938-47942 NN denotes pGL3
T28091 47937-47938 HYPH denotes -
T28092 47960-47967 NN denotes plasmid
T28093 47943-47950 NN denotes Control
T28094 47951-47959 NN denotes reporter
T28095 48102-48108 VBN denotes gifted
T28096 47967-47969 , denotes ,
T28097 47969-47974 WDT denotes which
T28098 47975-47983 VBZ denotes contains
T28099 47984-47988 CD denotes five
T28100 47989-47995 NNS denotes copies
T28101 47996-47998 IN denotes of
T28102 47999-48002 DT denotes the
T28103 48024-48032 NN denotes sequence
T28104 48003-48007 NN denotes GAL4
T28105 48008-48011 NN denotes DNA
T28106 48012-48023 NN denotes recognition
T28107 48033-48043 VBN denotes positioned
T28108 48044-48055 RB denotes immediately
T28109 48056-48064 IN denotes upstream
T28110 48065-48067 IN denotes of
T28111 48068-48072 NN denotes SV40
T28112 48081-48089 NN denotes promoter
T28113 48073-48080 JJ denotes minimal
T28114 48089-48091 , denotes ,
T28115 48091-48094 VBD denotes was
T28116 48095-48101 RB denotes kindly
T28117 48109-48113 IN denotes from
T28118 48114-48117 NNP denotes Dr.
T28119 48121-48128 NNP denotes Noguchi
T28120 48118-48120 NNP denotes T.
T28121 48129-48132 CC denotes and
T28122 48133-48137 VBN denotes used
T28123 48138-48141 IN denotes for
T28124 48142-48150 NN denotes analysis
T28125 48151-48153 IN denotes of
T28126 48154-48157 DT denotes the
T28127 48174-48182 NN denotes activity
T28128 48158-48173 JJ denotes transcriptional
T28129 48183-48185 IN denotes of
T28130 48186-48188 NN denotes mr
T28131 48189-48190 NN denotes s
T28132 48188-48189 HYPH denotes -
T28133 48191-48192 -LRB- denotes [
T28134 48192-48194 CD denotes 50
T28135 48194-48195 -RRB- denotes ]
T28136 48195-48196 . denotes .
T28137 48196-48332 sentence denotes To generate effector plasmids, various deletion fragments were produced by PCR reaction and subcloned into pGBKT7 vector, respectively.
T28138 48197-48199 TO denotes To
T28139 48200-48208 VB denotes generate
T28140 48260-48268 VBN denotes produced
T28141 48209-48217 NN denotes effector
T28142 48218-48226 NNS denotes plasmids
T28143 48226-48228 , denotes ,
T28144 48228-48235 JJ denotes various
T28145 48245-48254 NNS denotes fragments
T28146 48236-48244 NN denotes deletion
T28147 48255-48259 VBD denotes were
T28148 48269-48271 IN denotes by
T28149 48272-48275 NN denotes PCR
T28150 48276-48284 NN denotes reaction
T28151 48285-48288 CC denotes and
T28152 48289-48298 VBN denotes subcloned
T28153 48299-48303 IN denotes into
T28154 48304-48310 NN denotes pGBKT7
T28155 48311-48317 NN denotes vector
T28156 48317-48319 , denotes ,
T28157 48319-48331 RB denotes respectively
T28158 48331-48332 . denotes .
T28159 48332-48446 sentence denotes These fragments were digested with the sequence, which encodes GAL4 DNA binding domain, and inserted into pcDNA3.
T28160 48333-48338 DT denotes These
T28161 48339-48348 NNS denotes fragments
T28162 48354-48362 VBN denotes digested
T28163 48349-48353 VBD denotes were
T28164 48363-48367 IN denotes with
T28165 48368-48371 DT denotes the
T28166 48372-48380 NN denotes sequence
T28167 48380-48382 , denotes ,
T28168 48382-48387 WDT denotes which
T28169 48388-48395 VBZ denotes encodes
T28170 48396-48400 NN denotes GAL4
T28171 48413-48419 NN denotes domain
T28172 48401-48404 NN denotes DNA
T28173 48405-48412 NN denotes binding
T28174 48419-48421 , denotes ,
T28175 48421-48424 CC denotes and
T28176 48425-48433 VBN denotes inserted
T28177 48434-48438 IN denotes into
T28178 48439-48445 NN denotes pcDNA3
T28179 48445-48446 . denotes .
T28180 48446-48599 sentence denotes We transfected 0.1 μg of reporter plasmid DNA and 2 μg of the expression vector DNA per 6 cm dish into HEK293T cells using Fugene6 transfection reagent.
T28181 48447-48449 PRP denotes We
T28182 48450-48461 VBD denotes transfected
T28183 48462-48465 CD denotes 0.1
T28184 48466-48468 NN denotes μg
T28185 48469-48471 IN denotes of
T28186 48472-48480 NN denotes reporter
T28187 48489-48492 NN denotes DNA
T28188 48481-48488 NN denotes plasmid
T28189 48493-48496 CC denotes and
T28190 48497-48498 CD denotes 2
T28191 48499-48501 NN denotes μg
T28192 48502-48504 IN denotes of
T28193 48505-48508 DT denotes the
T28194 48527-48530 NN denotes DNA
T28195 48509-48519 NN denotes expression
T28196 48520-48526 NN denotes vector
T28197 48531-48534 IN denotes per
T28198 48535-48536 CD denotes 6
T28199 48537-48539 NN denotes cm
T28200 48540-48544 NN denotes dish
T28201 48545-48549 IN denotes into
T28202 48550-48557 NN denotes HEK293T
T28203 48558-48563 NNS denotes cells
T28204 48564-48569 VBG denotes using
T28205 48570-48577 NN denotes Fugene6
T28206 48591-48598 NN denotes reagent
T28207 48578-48590 NN denotes transfection
T28208 48598-48599 . denotes .
T28209 48599-48657 sentence denotes We analyzed luciferase activity 48 hr after transfection.
T28210 48600-48602 PRP denotes We
T28211 48603-48611 VBD denotes analyzed
T28212 48612-48622 NN denotes luciferase
T28213 48623-48631 NN denotes activity
T28214 48632-48634 CD denotes 48
T28215 48635-48637 NN denotes hr
T28216 48638-48643 IN denotes after
T28217 48644-48656 NN denotes transfection
T28218 48656-48657 . denotes .
T28701 9160-9162 NN denotes mr
T28702 9163-9164 NN denotes s
T28703 9162-9163 HYPH denotes -
T28704 9165-9175 NN denotes nucleotide
T28705 9191-9200 NNS denotes sequences
T28706 9176-9179 CC denotes and
T28707 9180-9185 NN denotes amino
T28708 9186-9190 NN denotes acid
T28709 9200-9201 . denotes .
T28710 9201-9248 sentence denotes (A) mr-s nucleotide and amino acids sequences.
T28711 9202-9203 -LRB- denotes (
T28712 9203-9204 LS denotes A
T28713 9238-9247 NNS denotes sequences
T28714 9204-9205 -RRB- denotes )
T28715 9206-9208 NN denotes mr
T28716 9209-9210 NN denotes s
T28717 9208-9209 HYPH denotes -
T28718 9211-9221 NN denotes nucleotide
T28719 9222-9225 CC denotes and
T28720 9226-9231 NN denotes amino
T28721 9232-9237 NNS denotes acids
T28722 9247-9248 . denotes .
T28723 9248-9363 sentence denotes Boxed amino acids are the SAM domain sequence and the dashed box indicates a putative nuclear localization signal.
T28724 9249-9254 VBN denotes Boxed
T28725 9261-9266 NNS denotes acids
T28726 9255-9260 NN denotes amino
T28727 9267-9270 VBP denotes are
T28728 9271-9274 DT denotes the
T28729 9286-9294 NN denotes sequence
T28730 9275-9278 NN denotes SAM
T28731 9279-9285 NN denotes domain
T28732 9295-9298 CC denotes and
T28733 9299-9302 DT denotes the
T28734 9310-9313 NN denotes box
T28735 9303-9309 JJ denotes dashed
T28736 9314-9323 VBZ denotes indicates
T28737 9324-9325 DT denotes a
T28738 9356-9362 NN denotes signal
T28739 9326-9334 JJ denotes putative
T28740 9335-9342 JJ denotes nuclear
T28741 9343-9355 NN denotes localization
T28742 9362-9363 . denotes .
T28743 9363-9434 sentence denotes The underline indicates a putative polyadenylation termination signal.
T28744 9364-9367 DT denotes The
T28745 9368-9377 NN denotes underline
T28746 9378-9387 VBZ denotes indicates
T28747 9388-9389 DT denotes a
T28748 9427-9433 NN denotes signal
T28749 9390-9398 JJ denotes putative
T28750 9399-9414 NN denotes polyadenylation
T28751 9415-9426 NN denotes termination
T28752 9433-9434 . denotes .
T28753 9434-9508 sentence denotes (B) Alignment of SAM domain sequences for SAM domain-containing proteins.
T28754 9435-9436 -LRB- denotes (
T28755 9436-9437 LS denotes B
T28756 9439-9448 NN denotes Alignment
T28757 9437-9438 -RRB- denotes )
T28758 9449-9451 IN denotes of
T28759 9452-9455 NN denotes SAM
T28760 9456-9462 NN denotes domain
T28761 9463-9472 NNS denotes sequences
T28762 9473-9476 IN denotes for
T28763 9477-9480 NN denotes SAM
T28764 9481-9487 NN denotes domain
T28765 9488-9498 VBG denotes containing
T28766 9487-9488 HYPH denotes -
T28767 9499-9507 NN denotes proteins
T28768 9507-9508 . denotes .
T28769 9508-9549 sentence denotes The five alpha helices are marked H1-H5.
T28770 9509-9512 DT denotes The
T28771 9524-9531 NNS denotes helices
T28772 9513-9517 CD denotes five
T28773 9518-9523 NN denotes alpha
T28774 9536-9542 VBN denotes marked
T28775 9532-9535 VBP denotes are
T28776 9543-9545 NN denotes H1
T28777 9545-9546 SYM denotes -
T28778 9546-9548 NN denotes H5
T28779 9548-9549 . denotes .
T28780 9549-9673 sentence denotes Conserved amino acid residues are shown with a dark shadow and functionally similar residues are shown with a light shadow.
T28781 9550-9559 VBN denotes Conserved
T28782 9571-9579 NNS denotes residues
T28783 9560-9565 NN denotes amino
T28784 9566-9570 NN denotes acid
T28785 9584-9589 VBN denotes shown
T28786 9580-9583 VBP denotes are
T28787 9590-9594 IN denotes with
T28788 9595-9596 DT denotes a
T28789 9602-9608 NN denotes shadow
T28790 9597-9601 JJ denotes dark
T28791 9609-9612 CC denotes and
T28792 9613-9625 RB denotes functionally
T28793 9626-9633 JJ denotes similar
T28794 9634-9642 NNS denotes residues
T28795 9647-9652 VBN denotes shown
T28796 9643-9646 VBP denotes are
T28797 9653-9657 IN denotes with
T28798 9658-9659 DT denotes a
T28799 9666-9672 NN denotes shadow
T28800 9660-9665 JJ denotes light
T28801 9672-9673 . denotes .
T28802 9673-9743 sentence denotes The sites that were targeted for mutagenesis are indicated by arrows.
T28803 9674-9677 DT denotes The
T28804 9678-9683 NNS denotes sites
T28805 9723-9732 VBN denotes indicated
T28806 9684-9688 WDT denotes that
T28807 9694-9702 VBN denotes targeted
T28808 9689-9693 VBD denotes were
T28809 9703-9706 IN denotes for
T28810 9707-9718 NN denotes mutagenesis
T28811 9719-9722 VBP denotes are
T28812 9733-9735 IN denotes by
T28813 9736-9742 NNS denotes arrows
T28814 9742-9743 . denotes .
T28815 9743-9800 sentence denotes (C) Phylogenetic tree of SAM domain-containing proteins.
T28816 9744-9745 -LRB- denotes (
T28817 9745-9746 LS denotes C
T28818 9761-9765 NN denotes tree
T28819 9746-9747 -RRB- denotes )
T28820 9748-9760 JJ denotes Phylogenetic
T28821 9766-9768 IN denotes of
T28822 9769-9772 NN denotes SAM
T28823 9773-9779 NN denotes domain
T28824 9780-9790 VBG denotes containing
T28825 9779-9780 HYPH denotes -
T28826 9791-9799 NN denotes proteins
T28827 9799-9800 . denotes .
T28828 9800-9884 sentence denotes Amino acid sequences were analyzed by the neighbor-joining method in MacVector 7.2.
T28829 9801-9806 NN denotes Amino
T28830 9807-9811 NN denotes acid
T28831 9812-9821 NNS denotes sequences
T28832 9827-9835 VBN denotes analyzed
T28833 9822-9826 VBD denotes were
T28834 9836-9838 IN denotes by
T28835 9839-9842 DT denotes the
T28836 9860-9866 NN denotes method
T28837 9843-9851 NN denotes neighbor
T28838 9852-9859 VBG denotes joining
T28839 9851-9852 HYPH denotes -
T28840 9867-9869 IN denotes in
T28841 9870-9879 NNP denotes MacVector
T28842 9880-9883 CD denotes 7.2
T28843 9883-9884 . denotes .
T28844 9884-9950 sentence denotes Branch lengths reflect the mean number of substitutions per site.
T28845 9885-9891 NN denotes Branch
T28846 9892-9899 NNS denotes lengths
T28847 9900-9907 VBP denotes reflect
T28848 9908-9911 DT denotes the
T28849 9917-9923 NN denotes number
T28850 9912-9916 NN denotes mean
T28851 9924-9926 IN denotes of
T28852 9927-9940 NNS denotes substitutions
T28853 9941-9944 IN denotes per
T28854 9945-9949 NN denotes site
T28855 9949-9950 . denotes .
T28856 9950-10061 sentence denotes (D) Schematic comparison of the amino acid sequences for mouse, rat, human, chick and zebrafish mr-s proteins.
T28857 9951-9952 -LRB- denotes (
T28858 9952-9953 LS denotes D
T28859 9965-9975 NN denotes comparison
T28860 9953-9954 -RRB- denotes )
T28861 9955-9964 JJ denotes Schematic
T28862 9976-9978 IN denotes of
T28863 9979-9982 DT denotes the
T28864 9994-10003 NNS denotes sequences
T28865 9983-9988 NN denotes amino
T28866 9989-9993 NN denotes acid
T28867 10004-10007 IN denotes for
T28868 10008-10013 NN denotes mouse
T28869 10052-10060 NN denotes proteins
T28870 10013-10015 , denotes ,
T28871 10015-10018 NN denotes rat
T28872 10018-10020 , denotes ,
T28873 10020-10025 JJ denotes human
T28874 10025-10027 , denotes ,
T28875 10027-10032 NN denotes chick
T28876 10033-10036 CC denotes and
T28877 10037-10046 NN denotes zebrafish
T28878 10047-10049 NN denotes mr
T28879 10050-10051 NN denotes s
T28880 10049-10050 HYPH denotes -
T28881 10060-10061 . denotes .
T28882 10061-10181 sentence denotes The percent similarity of the SAM domains and other regions to the corresponding regions of the mouse protein is shown.
T28883 10062-10065 DT denotes The
T28884 10074-10084 NN denotes similarity
T28885 10066-10073 NN denotes percent
T28886 10175-10180 VBN denotes shown
T28887 10085-10087 IN denotes of
T28888 10088-10091 DT denotes the
T28889 10096-10103 NNS denotes domains
T28890 10092-10095 NN denotes SAM
T28891 10104-10107 CC denotes and
T28892 10108-10113 JJ denotes other
T28893 10114-10121 NNS denotes regions
T28894 10122-10124 IN denotes to
T28895 10125-10128 DT denotes the
T28896 10143-10150 NNS denotes regions
T28897 10129-10142 VBG denotes corresponding
T28898 10151-10153 IN denotes of
T28899 10154-10157 DT denotes the
T28900 10164-10171 NN denotes protein
T28901 10158-10163 NN denotes mouse
T28902 10172-10174 VBZ denotes is
T28903 10180-10181 . denotes .
T28904 10181-10255 sentence denotes Overall sequence similarity with the mouse protein is shown on the right.
T28905 10182-10189 JJ denotes Overall
T28906 10199-10209 NN denotes similarity
T28907 10190-10198 NN denotes sequence
T28908 10236-10241 VBN denotes shown
T28909 10210-10214 IN denotes with
T28910 10215-10218 DT denotes the
T28911 10225-10232 NN denotes protein
T28912 10219-10224 NN denotes mouse
T28913 10233-10235 VBZ denotes is
T28914 10242-10244 IN denotes on
T28915 10245-10248 DT denotes the
T28916 10249-10254 NN denotes right
T28917 10254-10255 . denotes .
T29458 11423-11425 NN denotes mr
T29459 11426-11427 NN denotes s
T29460 11425-11426 HYPH denotes -
T29461 11428-11438 NN denotes expression
T29462 11439-11441 IN denotes in
T29463 11442-11452 VBG denotes developing
T29464 11459-11465 NN denotes retina
T29465 11453-11458 NN denotes mouse
T29466 11466-11469 CC denotes and
T29467 11470-11476 JJ denotes pineal
T29468 11477-11482 NN denotes gland
T29469 11482-11483 . denotes .
T29470 11483-11545 sentence denotes (A-G) mr-s expression during development of the mouse retina.
T29471 11484-11485 -LRB- denotes (
T29472 11485-11486 LS denotes A
T29473 11495-11505 NN denotes expression
T29474 11486-11487 SYM denotes -
T29475 11487-11488 LS denotes G
T29476 11488-11489 -RRB- denotes )
T29477 11490-11492 NN denotes mr
T29478 11493-11494 NN denotes s
T29479 11492-11493 HYPH denotes -
T29480 11506-11512 IN denotes during
T29481 11513-11524 NN denotes development
T29482 11525-11527 IN denotes of
T29483 11528-11531 DT denotes the
T29484 11538-11544 NN denotes retina
T29485 11532-11537 NN denotes mouse
T29486 11544-11545 . denotes .
T29487 11545-11615 sentence denotes The in situ hybridization signal of mr-s was not detected at E13 (A).
T29488 11546-11549 DT denotes The
T29489 11572-11578 NN denotes signal
T29490 11550-11552 FW denotes in
T29491 11553-11557 FW denotes situ
T29492 11558-11571 NN denotes hybridization
T29493 11595-11603 VBN denotes detected
T29494 11579-11581 IN denotes of
T29495 11582-11584 NN denotes mr
T29496 11585-11586 NN denotes s
T29497 11584-11585 HYPH denotes -
T29498 11587-11590 VBD denotes was
T29499 11591-11594 RB denotes not
T29500 11604-11606 IN denotes at
T29501 11607-11610 NN denotes E13
T29502 11611-11612 -LRB- denotes (
T29503 11612-11613 NN denotes A
T29504 11613-11614 -RRB- denotes )
T29505 11614-11615 . denotes .
T29506 11615-11692 sentence denotes The signal (arrow) was first detected in the outer aspect of NBL at E18 (B).
T29507 11616-11619 DT denotes The
T29508 11620-11626 NN denotes signal
T29509 11645-11653 VBN denotes detected
T29510 11627-11628 -LRB- denotes (
T29511 11628-11633 NN denotes arrow
T29512 11633-11634 -RRB- denotes )
T29513 11635-11638 VBD denotes was
T29514 11639-11644 RB denotes first
T29515 11654-11656 IN denotes in
T29516 11657-11660 DT denotes the
T29517 11667-11673 NN denotes aspect
T29518 11661-11666 JJ denotes outer
T29519 11674-11676 IN denotes of
T29520 11677-11680 NN denotes NBL
T29521 11681-11683 IN denotes at
T29522 11684-11687 NN denotes E18
T29523 11688-11689 -LRB- denotes (
T29524 11689-11690 NN denotes B
T29525 11690-11691 -RRB- denotes )
T29526 11691-11692 . denotes .
T29527 11692-11822 sentence denotes A strong mr-s signal was detected in outer layer of the retina at P3-P6, and then the signal decreased in the adult retina (E-G).
T29528 11693-11694 DT denotes A
T29529 11707-11713 NN denotes signal
T29530 11695-11701 JJ denotes strong
T29531 11702-11704 NN denotes mr
T29532 11705-11706 NN denotes s
T29533 11704-11705 HYPH denotes -
T29534 11718-11726 VBN denotes detected
T29535 11714-11717 VBD denotes was
T29536 11727-11729 IN denotes in
T29537 11730-11735 JJ denotes outer
T29538 11736-11741 NN denotes layer
T29539 11742-11744 IN denotes of
T29540 11745-11748 DT denotes the
T29541 11749-11755 NN denotes retina
T29542 11756-11758 IN denotes at
T29543 11759-11761 NN denotes P3
T29544 11761-11762 SYM denotes -
T29545 11762-11764 NN denotes P6
T29546 11764-11766 , denotes ,
T29547 11766-11769 CC denotes and
T29548 11770-11774 RB denotes then
T29549 11786-11795 VBD denotes decreased
T29550 11775-11778 DT denotes the
T29551 11779-11785 NN denotes signal
T29552 11796-11798 IN denotes in
T29553 11799-11802 DT denotes the
T29554 11809-11815 NN denotes retina
T29555 11803-11808 JJ denotes adult
T29556 11816-11817 -LRB- denotes (
T29557 11817-11818 NN denotes E
T29558 11818-11819 SYM denotes -
T29559 11819-11820 NN denotes G
T29560 11820-11821 -RRB- denotes )
T29561 11821-11822 . denotes .
T29562 11822-11879 sentence denotes Control with the sense probe in E18 retina is shown (C).
T29563 11823-11830 NN denotes Control
T29564 11869-11874 VBN denotes shown
T29565 11831-11835 IN denotes with
T29566 11836-11839 DT denotes the
T29567 11846-11851 NN denotes probe
T29568 11840-11845 NN denotes sense
T29569 11852-11854 IN denotes in
T29570 11855-11858 NN denotes E18
T29571 11859-11865 NN denotes retina
T29572 11866-11868 VBZ denotes is
T29573 11875-11876 -LRB- denotes (
T29574 11876-11877 NN denotes C
T29575 11877-11878 -RRB- denotes )
T29576 11878-11879 . denotes .
T29577 11879-11898 sentence denotes Scale bar, 100 μm.
T29578 11880-11885 NN denotes Scale
T29579 11886-11889 NN denotes bar
T29580 11889-11891 , denotes ,
T29581 11891-11894 CD denotes 100
T29582 11895-11897 NN denotes μm
T29583 11897-11898 . denotes .
T29584 11898-11967 sentence denotes (H) Northern blot analysis of mr-s expression in adult mouse organs.
T29585 11899-11900 -LRB- denotes (
T29586 11900-11901 LS denotes H
T29587 11917-11925 NN denotes analysis
T29588 11901-11902 -RRB- denotes )
T29589 11903-11911 NNP denotes Northern
T29590 11912-11916 NN denotes blot
T29591 11926-11928 IN denotes of
T29592 11929-11931 NN denotes mr
T29593 11932-11933 NN denotes s
T29594 11931-11932 HYPH denotes -
T29595 11934-11944 NN denotes expression
T29596 11945-11947 IN denotes in
T29597 11948-11953 JJ denotes adult
T29598 11960-11966 NNS denotes organs
T29599 11954-11959 NN denotes mouse
T29600 11966-11967 . denotes .
T29601 11967-12015 sentence denotes The arrow corresponds to 2.2kb mr-s transcript.
T29602 11968-11971 DT denotes The
T29603 11972-11977 NN denotes arrow
T29604 11978-11989 VBZ denotes corresponds
T29605 11990-11992 IN denotes to
T29606 11993-11998 NN denotes 2.2kb
T29607 12004-12014 NN denotes transcript
T29608 11999-12001 NN denotes mr
T29609 12002-12003 NN denotes s
T29610 12001-12002 HYPH denotes -
T29611 12014-12015 . denotes .
T29612 12015-12243 sentence denotes (I) RT-PCR analysis of total RNAs extracted from E13 whole embryo, P0 whole body (except for the eye), P7 retina, P7 pineal gland, P7 brain, P7 liver, adult retina, adult pineal gland, adult brain and adult liver, respectively.
T29613 12016-12017 -LRB- denotes (
T29614 12017-12018 LS denotes I
T29615 12027-12035 NN denotes analysis
T29616 12018-12019 -RRB- denotes )
T29617 12020-12022 NN denotes RT
T29618 12023-12026 NN denotes PCR
T29619 12022-12023 HYPH denotes -
T29620 12036-12038 IN denotes of
T29621 12039-12044 JJ denotes total
T29622 12045-12049 NNS denotes RNAs
T29623 12050-12059 VBN denotes extracted
T29624 12060-12064 IN denotes from
T29625 12065-12068 NN denotes E13
T29626 12075-12081 NN denotes embryo
T29627 12069-12074 JJ denotes whole
T29628 12081-12083 , denotes ,
T29629 12083-12085 NN denotes P0
T29630 12092-12096 NN denotes body
T29631 12086-12091 JJ denotes whole
T29632 12097-12098 -LRB- denotes (
T29633 12098-12104 IN denotes except
T29634 12105-12108 IN denotes for
T29635 12109-12112 DT denotes the
T29636 12113-12116 NN denotes eye
T29637 12116-12117 -RRB- denotes )
T29638 12117-12119 , denotes ,
T29639 12119-12121 NN denotes P7
T29640 12122-12128 NN denotes retina
T29641 12128-12130 , denotes ,
T29642 12130-12132 NN denotes P7
T29643 12140-12145 NN denotes gland
T29644 12133-12139 JJ denotes pineal
T29645 12145-12147 , denotes ,
T29646 12147-12149 NN denotes P7
T29647 12150-12155 NN denotes brain
T29648 12155-12157 , denotes ,
T29649 12157-12159 NN denotes P7
T29650 12160-12165 NN denotes liver
T29651 12165-12167 , denotes ,
T29652 12167-12172 JJ denotes adult
T29653 12173-12179 NN denotes retina
T29654 12179-12181 , denotes ,
T29655 12181-12186 JJ denotes adult
T29656 12194-12199 NN denotes gland
T29657 12187-12193 JJ denotes pineal
T29658 12199-12201 , denotes ,
T29659 12201-12206 JJ denotes adult
T29660 12207-12212 NN denotes brain
T29661 12213-12216 CC denotes and
T29662 12217-12222 JJ denotes adult
T29663 12223-12228 NN denotes liver
T29664 12228-12230 , denotes ,
T29665 12230-12242 RB denotes respectively
T29666 12242-12243 . denotes .
T29667 12243-12398 sentence denotes RPE, retinal pigment epithelium; NR, neural retina; NBL, neuroblastic layer; GCL, ganglion cell layer; ONL, outer nuclear layer; INL, inner nuclear layer.
T29668 12244-12247 NN denotes RPE
T29669 12247-12249 , denotes ,
T29670 12249-12256 JJ denotes retinal
T29671 12265-12275 NN denotes epithelium
T29672 12257-12264 NN denotes pigment
T29673 12275-12276 : denotes ;
T29674 12277-12279 NN denotes NR
T29675 12279-12281 , denotes ,
T29676 12281-12287 JJ denotes neural
T29677 12288-12294 NN denotes retina
T29678 12294-12295 : denotes ;
T29679 12296-12299 NN denotes NBL
T29680 12299-12301 , denotes ,
T29681 12301-12313 JJ denotes neuroblastic
T29682 12314-12319 NN denotes layer
T29683 12319-12320 : denotes ;
T29684 12321-12324 NN denotes GCL
T29685 12324-12326 , denotes ,
T29686 12326-12334 NN denotes ganglion
T29687 12335-12339 NN denotes cell
T29688 12340-12345 NN denotes layer
T29689 12345-12346 : denotes ;
T29690 12347-12350 NN denotes ONL
T29691 12350-12352 , denotes ,
T29692 12352-12357 JJ denotes outer
T29693 12366-12371 NN denotes layer
T29694 12358-12365 JJ denotes nuclear
T29695 12371-12372 : denotes ;
T29696 12373-12376 NN denotes INL
T29697 12376-12378 , denotes ,
T29698 12378-12383 JJ denotes inner
T29699 12392-12397 NN denotes layer
T29700 12384-12391 JJ denotes nuclear
T29701 12397-12398 . denotes .
T30251 14998-15001 DT denotes The
T30252 15002-15015 NN denotes transcription
T30253 15027-15036 VBN denotes regulated
T30254 15016-15018 IN denotes of
T30255 15019-15021 NN denotes mr
T30256 15022-15023 NN denotes s
T30257 15021-15022 HYPH denotes -
T30258 15024-15026 VBZ denotes is
T30259 15037-15039 IN denotes by
T30260 15040-15043 NN denotes Crx
T30261 15043-15044 . denotes .
T30262 15044-15167 sentence denotes (A, B) In situ hybridization using a probe for mouse mr-s was performed on the wild-type (A) and Crx KO retinas (B) at P5.
T30263 15045-15046 -LRB- denotes (
T30264 15046-15047 LS denotes A
T30265 15107-15116 VBN denotes performed
T30266 15047-15049 , denotes ,
T30267 15049-15050 LS denotes B
T30268 15050-15051 -RRB- denotes )
T30269 15052-15054 FW denotes In
T30270 15055-15059 FW denotes situ
T30271 15060-15073 NN denotes hybridization
T30272 15074-15079 VBG denotes using
T30273 15080-15081 DT denotes a
T30274 15082-15087 NN denotes probe
T30275 15088-15091 IN denotes for
T30276 15092-15097 NN denotes mouse
T30277 15101-15102 NN denotes s
T30278 15098-15100 NN denotes mr
T30279 15100-15101 HYPH denotes -
T30280 15103-15106 VBD denotes was
T30281 15117-15119 IN denotes on
T30282 15120-15123 DT denotes the
T30283 15149-15156 NNS denotes retinas
T30284 15124-15128 JJ denotes wild
T30285 15129-15133 NN denotes type
T30286 15128-15129 HYPH denotes -
T30287 15134-15135 -LRB- denotes (
T30288 15135-15136 NN denotes A
T30289 15136-15137 -RRB- denotes )
T30290 15138-15141 CC denotes and
T30291 15142-15145 NN denotes Crx
T30292 15146-15148 NN denotes KO
T30293 15157-15158 -LRB- denotes (
T30294 15158-15159 NN denotes B
T30295 15159-15160 -RRB- denotes )
T30296 15161-15163 IN denotes at
T30297 15164-15166 NN denotes P5
T30298 15166-15167 . denotes .
T30299 15167-15233 sentence denotes mr-s expression was drastically reduced in the Crx KO retina (B).
T30300 15168-15170 NN denotes mr
T30301 15171-15172 NN denotes s
T30302 15170-15171 HYPH denotes -
T30303 15173-15183 NN denotes expression
T30304 15200-15207 VBN denotes reduced
T30305 15184-15187 VBD denotes was
T30306 15188-15199 RB denotes drastically
T30307 15208-15210 IN denotes in
T30308 15211-15214 DT denotes the
T30309 15222-15228 NN denotes retina
T30310 15215-15218 NN denotes Crx
T30311 15219-15221 NN denotes KO
T30312 15229-15230 -LRB- denotes (
T30313 15230-15231 NN denotes B
T30314 15231-15232 -RRB- denotes )
T30315 15232-15233 . denotes .
T30316 15233-15252 sentence denotes Scale bar, 100 μm.
T30317 15234-15239 NN denotes Scale
T30318 15240-15243 NN denotes bar
T30319 15243-15245 , denotes ,
T30320 15245-15248 CD denotes 100
T30321 15249-15251 NN denotes μm
T30322 15251-15252 . denotes .
T30323 15252-15353 sentence denotes (C) RT-PCR analysis of total RNAs extracted from the pineal glands of P5 wild-type and Crx KO mouse.
T30324 15253-15254 -LRB- denotes (
T30325 15254-15255 LS denotes C
T30326 15264-15272 NN denotes analysis
T30327 15255-15256 -RRB- denotes )
T30328 15257-15259 NN denotes RT
T30329 15260-15263 NN denotes PCR
T30330 15259-15260 HYPH denotes -
T30331 15273-15275 IN denotes of
T30332 15276-15281 JJ denotes total
T30333 15282-15286 NNS denotes RNAs
T30334 15287-15296 VBN denotes extracted
T30335 15297-15301 IN denotes from
T30336 15302-15305 DT denotes the
T30337 15313-15319 NNS denotes glands
T30338 15306-15312 JJ denotes pineal
T30339 15320-15322 IN denotes of
T30340 15323-15325 NN denotes P5
T30341 15347-15352 NN denotes mouse
T30342 15326-15330 JJ denotes wild
T30343 15331-15335 NN denotes type
T30344 15330-15331 HYPH denotes -
T30345 15336-15339 CC denotes and
T30346 15340-15343 NN denotes Crx
T30347 15344-15346 NN denotes KO
T30348 15352-15353 . denotes .
T30349 15353-15476 sentence denotes The upper and lower lanes show PCR products amplified by the primer pairs specific for mr-s and G3PDH cDNAs, respectively.
T30350 15354-15357 DT denotes The
T30351 15374-15379 NNS denotes lanes
T30352 15358-15363 JJ denotes upper
T30353 15364-15367 CC denotes and
T30354 15368-15373 JJ denotes lower
T30355 15380-15384 VBP denotes show
T30356 15385-15388 NN denotes PCR
T30357 15389-15397 NNS denotes products
T30358 15398-15407 VBN denotes amplified
T30359 15408-15410 IN denotes by
T30360 15411-15414 DT denotes the
T30361 15422-15427 NNS denotes pairs
T30362 15415-15421 NN denotes primer
T30363 15428-15436 JJ denotes specific
T30364 15437-15440 IN denotes for
T30365 15441-15443 NN denotes mr
T30366 15444-15445 NN denotes s
T30367 15443-15444 HYPH denotes -
T30368 15446-15449 CC denotes and
T30369 15450-15455 NN denotes G3PDH
T30370 15456-15461 NNS denotes cDNAs
T30371 15461-15463 , denotes ,
T30372 15463-15475 RB denotes respectively
T30373 15475-15476 . denotes .
T30374 15476-15520 sentence denotes Water was used for control RT-PCR reaction.
T30375 15477-15482 NN denotes Water
T30376 15487-15491 VBN denotes used
T30377 15483-15486 VBD denotes was
T30378 15492-15495 IN denotes for
T30379 15496-15503 NN denotes control
T30380 15511-15519 NN denotes reaction
T30381 15504-15506 NN denotes RT
T30382 15507-15510 NN denotes PCR
T30383 15506-15507 HYPH denotes -
T30384 15519-15520 . denotes .
T30385 15520-15565 sentence denotes (D-F) Crx transactivates mr-s transcription.
T30386 15521-15522 -LRB- denotes (
T30387 15522-15523 LS denotes D
T30388 15531-15545 VBZ denotes transactivates
T30389 15523-15524 SYM denotes -
T30390 15524-15525 LS denotes F
T30391 15525-15526 -RRB- denotes )
T30392 15527-15530 NN denotes Crx
T30393 15546-15548 NN denotes mr
T30394 15549-15550 NN denotes s
T30395 15548-15549 HYPH denotes -
T30396 15551-15564 NN denotes transcription
T30397 15564-15565 . denotes .
T30398 15565-15619 sentence denotes Reporter plasmids for the luciferase assay are shown.
T30399 15566-15574 NN denotes Reporter
T30400 15575-15583 NNS denotes plasmids
T30401 15613-15618 VBN denotes shown
T30402 15584-15587 IN denotes for
T30403 15588-15591 DT denotes the
T30404 15603-15608 NN denotes assay
T30405 15592-15602 NN denotes luciferase
T30406 15609-15612 VBP denotes are
T30407 15618-15619 . denotes .
T30408 15619-15663 sentence denotes Blue boxes represent Crx binding sites (D).
T30409 15620-15624 JJ denotes Blue
T30410 15625-15630 NNS denotes boxes
T30411 15631-15640 VBP denotes represent
T30412 15641-15644 NN denotes Crx
T30413 15653-15658 NNS denotes sites
T30414 15645-15652 NN denotes binding
T30415 15659-15660 -LRB- denotes (
T30416 15660-15661 NN denotes D
T30417 15661-15662 -RRB- denotes )
T30418 15662-15663 . denotes .
T30419 15663-15796 sentence denotes Relative activity of the luciferase is indicated when Pro1.2k was co-transfected with Crx, Otx2, Nrl, and Crx+Nrl, respectively (E).
T30420 15664-15672 JJ denotes Relative
T30421 15673-15681 NN denotes activity
T30422 15703-15712 VBN denotes indicated
T30423 15682-15684 IN denotes of
T30424 15685-15688 DT denotes the
T30425 15689-15699 NN denotes luciferase
T30426 15700-15702 VBZ denotes is
T30427 15713-15717 WRB denotes when
T30428 15730-15744 VBN denotes co-transfected
T30429 15718-15725 NN denotes Pro1.2k
T30430 15726-15729 VBD denotes was
T30431 15745-15749 IN denotes with
T30432 15750-15753 NN denotes Crx
T30433 15753-15755 , denotes ,
T30434 15755-15759 NN denotes Otx2
T30435 15759-15761 , denotes ,
T30436 15761-15764 NN denotes Nrl
T30437 15764-15766 , denotes ,
T30438 15766-15769 CC denotes and
T30439 15770-15773 NN denotes Crx
T30440 15774-15777 NN denotes Nrl
T30441 15773-15774 SYM denotes +
T30442 15777-15779 , denotes ,
T30443 15779-15791 RB denotes respectively
T30444 15792-15793 -LRB- denotes (
T30445 15793-15794 NN denotes E
T30446 15794-15795 -RRB- denotes )
T30447 15795-15796 . denotes .
T30448 15796-15983 sentence denotes Fold activation is indicated when Pro1.2k, mut1259, mut198, mut72 and mut all were co-transfected with the Crx expression vector (Crx+) or the empty vector (Crx-) into HEK293T cells (F).
T30449 15797-15801 NN denotes Fold
T30450 15802-15812 NN denotes activation
T30451 15816-15825 VBN denotes indicated
T30452 15813-15815 VBZ denotes is
T30453 15826-15830 WRB denotes when
T30454 15880-15894 VBN denotes co-transfected
T30455 15831-15838 NN denotes Pro1.2k
T30456 15838-15840 , denotes ,
T30457 15840-15847 NN denotes mut1259
T30458 15847-15849 , denotes ,
T30459 15849-15855 NN denotes mut198
T30460 15855-15857 , denotes ,
T30461 15857-15862 NN denotes mut72
T30462 15863-15866 CC denotes and
T30463 15867-15870 NN denotes mut
T30464 15871-15874 NN denotes all
T30465 15875-15879 VBD denotes were
T30466 15895-15899 IN denotes with
T30467 15900-15903 DT denotes the
T30468 15919-15925 NN denotes vector
T30469 15904-15907 NN denotes Crx
T30470 15908-15918 NN denotes expression
T30471 15926-15927 -LRB- denotes (
T30472 15927-15930 NN denotes Crx
T30473 15930-15931 SYM denotes +
T30474 15931-15932 -RRB- denotes )
T30475 15933-15935 CC denotes or
T30476 15936-15939 DT denotes the
T30477 15946-15952 NN denotes vector
T30478 15940-15945 JJ denotes empty
T30479 15953-15954 -LRB- denotes (
T30480 15954-15957 NN denotes Crx
T30481 15957-15958 SYM denotes -
T30482 15958-15959 -RRB- denotes )
T30483 15960-15964 IN denotes into
T30484 15965-15972 NN denotes HEK293T
T30485 15973-15978 NNS denotes cells
T30486 15979-15980 -LRB- denotes (
T30487 15980-15981 NN denotes F
T30488 15981-15982 -RRB- denotes )
T30489 15982-15983 . denotes .
T30490 15983-16028 sentence denotes Error bars represent standard error of mean.
T30491 15984-15989 NN denotes Error
T30492 15990-15994 NNS denotes bars
T30493 15995-16004 VBP denotes represent
T30494 16005-16013 JJ denotes standard
T30495 16014-16019 NN denotes error
T30496 16020-16022 IN denotes of
T30497 16023-16027 NN denotes mean
T30498 16027-16028 . denotes .
T30892 20740-20747 NN denotes Summary
T30893 20748-20750 IN denotes of
T30894 20751-20756 NN denotes yeast
T30895 20768-20777 NN denotes screening
T30896 20757-20760 CD denotes two
T30897 20761-20767 NN denotes hybrid
T30898 20760-20761 HYPH denotes -
T30899 20778-20781 CC denotes and
T30900 20782-20786 NN denotes GAL4
T30901 20787-20792 NN denotes assay
T30902 20792-20793 . denotes .
T30903 20793-20898 sentence denotes (A) Full-length mr-s as a bait used in the yeast two-hybrid screening and positive clones are indicated.
T30904 20794-20795 -LRB- denotes (
T30905 20795-20796 LS denotes A
T30906 20888-20897 VBN denotes indicated
T30907 20796-20797 -RRB- denotes )
T30908 20798-20802 JJ denotes Full
T30909 20803-20809 NN denotes length
T30910 20802-20803 HYPH denotes -
T30911 20813-20814 NN denotes s
T30912 20810-20812 NN denotes mr
T30913 20812-20813 HYPH denotes -
T30914 20815-20817 IN denotes as
T30915 20818-20819 DT denotes a
T30916 20820-20824 NN denotes bait
T30917 20825-20829 VBN denotes used
T30918 20830-20832 IN denotes in
T30919 20833-20836 DT denotes the
T30920 20854-20863 NN denotes screening
T30921 20837-20842 NN denotes yeast
T30922 20843-20846 CD denotes two
T30923 20847-20853 NN denotes hybrid
T30924 20846-20847 HYPH denotes -
T30925 20864-20867 CC denotes and
T30926 20868-20876 JJ denotes positive
T30927 20877-20883 NNS denotes clones
T30928 20884-20887 VBP denotes are
T30929 20897-20898 . denotes .
T30930 20898-20981 sentence denotes Note that all of five mr-s clones identified in the screening contain SAM domains.
T30931 20899-20903 VB denotes Note
T30932 20904-20908 IN denotes that
T30933 20961-20968 VBP denotes contain
T30934 20909-20912 DT denotes all
T30935 20913-20915 IN denotes of
T30936 20916-20920 CD denotes five
T30937 20926-20932 NNS denotes clones
T30938 20921-20923 NN denotes mr
T30939 20924-20925 NN denotes s
T30940 20923-20924 HYPH denotes -
T30941 20933-20943 VBN denotes identified
T30942 20944-20946 IN denotes in
T30943 20947-20950 DT denotes the
T30944 20951-20960 NN denotes screening
T30945 20969-20972 NN denotes SAM
T30946 20973-20980 NNS denotes domains
T30947 20980-20981 . denotes .
T30948 20981-21061 sentence denotes (B) Schematic diagram of the mr-s fusion proteins used in the yeast GAL4 assay.
T30949 20982-20983 -LRB- denotes (
T30950 20983-20984 LS denotes B
T30951 20996-21003 NN denotes diagram
T30952 20984-20985 -RRB- denotes )
T30953 20986-20995 JJ denotes Schematic
T30954 21004-21006 IN denotes of
T30955 21007-21010 DT denotes the
T30956 21023-21031 NN denotes proteins
T30957 21011-21013 NN denotes mr
T30958 21014-21015 NN denotes s
T30959 21013-21014 HYPH denotes -
T30960 21016-21022 NN denotes fusion
T30961 21032-21036 VBN denotes used
T30962 21037-21039 IN denotes in
T30963 21040-21043 DT denotes the
T30964 21055-21060 NN denotes assay
T30965 21044-21049 NN denotes yeast
T30966 21050-21054 NN denotes GAL4
T30967 21060-21061 . denotes .
T30968 21061-21112 sentence denotes Black boxes represent the position of SAM domains.
T30969 21062-21067 JJ denotes Black
T30970 21068-21073 NNS denotes boxes
T30971 21074-21083 VBP denotes represent
T30972 21084-21087 DT denotes the
T30973 21088-21096 NN denotes position
T30974 21097-21099 IN denotes of
T30975 21100-21103 NN denotes SAM
T30976 21104-21111 NNS denotes domains
T30977 21111-21112 . denotes .
T30978 21112-21185 sentence denotes Relative levels of LacZ expression are shown on the right, respectively.
T30979 21113-21121 JJ denotes Relative
T30980 21122-21128 NNS denotes levels
T30981 21152-21157 VBN denotes shown
T30982 21129-21131 IN denotes of
T30983 21132-21136 NN denotes LacZ
T30984 21137-21147 NN denotes expression
T30985 21148-21151 VBP denotes are
T30986 21158-21160 IN denotes on
T30987 21161-21164 DT denotes the
T30988 21165-21170 NN denotes right
T30989 21170-21172 , denotes ,
T30990 21172-21184 RB denotes respectively
T30991 21184-21185 . denotes .
T30992 21185-21329 sentence denotes Note; ++ indicates an intense blue signal visible after 12hr of incubation at 37°C, + indicates a blue signal visible after 24hr of incubation.
T30993 21186-21190 NN denotes Note
T30994 21272-21281 VBZ denotes indicates
T30995 21190-21191 : denotes ;
T30996 21192-21194 SYM denotes ++
T30997 21195-21204 VBZ denotes indicates
T30998 21205-21207 DT denotes an
T30999 21221-21227 NN denotes signal
T31000 21208-21215 JJ denotes intense
T31001 21216-21220 JJ denotes blue
T31002 21228-21235 JJ denotes visible
T31003 21236-21241 IN denotes after
T31004 21242-21246 NN denotes 12hr
T31005 21247-21249 IN denotes of
T31006 21250-21260 NN denotes incubation
T31007 21261-21263 IN denotes at
T31008 21264-21266 CD denotes 37
T31009 21266-21268 NN denotes °C
T31010 21268-21270 , denotes ,
T31011 21270-21271 SYM denotes +
T31012 21282-21283 DT denotes a
T31013 21289-21295 NN denotes signal
T31014 21284-21288 JJ denotes blue
T31015 21296-21303 JJ denotes visible
T31016 21304-21309 IN denotes after
T31017 21310-21314 NN denotes 24hr
T31018 21315-21317 IN denotes of
T31019 21318-21328 NN denotes incubation
T31020 21328-21329 . denotes .
T31021 21329-21506 sentence denotes BD, binding domain; AD, activation domain; full, full-length mr-s; N, N-terminal portion of mr-s (amino acids 1 to 400); C, C-terminal portion of mr-s (amino acids 391 to 542).
T31022 21330-21332 NN denotes BD
T31023 21332-21334 , denotes ,
T31024 21334-21341 NN denotes binding
T31025 21342-21348 NN denotes domain
T31026 21348-21349 : denotes ;
T31027 21350-21352 NN denotes AD
T31028 21352-21354 , denotes ,
T31029 21354-21364 NN denotes activation
T31030 21365-21371 NN denotes domain
T31031 21371-21372 : denotes ;
T31032 21373-21377 JJ denotes full
T31033 21394-21395 NN denotes s
T31034 21377-21379 , denotes ,
T31035 21379-21383 JJ denotes full
T31036 21384-21390 NN denotes length
T31037 21383-21384 HYPH denotes -
T31038 21391-21393 NN denotes mr
T31039 21393-21394 HYPH denotes -
T31040 21395-21396 : denotes ;
T31041 21397-21398 NN denotes N
T31042 21398-21400 , denotes ,
T31043 21400-21401 NN denotes N
T31044 21402-21410 JJ denotes terminal
T31045 21401-21402 HYPH denotes -
T31046 21411-21418 NN denotes portion
T31047 21419-21421 IN denotes of
T31048 21422-21424 NN denotes mr
T31049 21425-21426 NN denotes s
T31050 21424-21425 HYPH denotes -
T31051 21427-21428 -LRB- denotes (
T31052 21440-21441 CD denotes 1
T31053 21428-21433 NN denotes amino
T31054 21434-21439 NNS denotes acids
T31055 21442-21444 IN denotes to
T31056 21445-21448 CD denotes 400
T31057 21448-21449 -RRB- denotes )
T31058 21449-21450 : denotes ;
T31059 21451-21452 NN denotes C
T31060 21452-21454 , denotes ,
T31061 21454-21455 NN denotes C
T31062 21456-21464 JJ denotes terminal
T31063 21455-21456 HYPH denotes -
T31064 21465-21472 NN denotes portion
T31065 21473-21475 IN denotes of
T31066 21476-21478 NN denotes mr
T31067 21479-21480 NN denotes s
T31068 21478-21479 HYPH denotes -
T31069 21481-21482 -LRB- denotes (
T31070 21494-21497 CD denotes 391
T31071 21482-21487 NN denotes amino
T31072 21488-21493 NNS denotes acids
T31073 21498-21500 IN denotes to
T31074 21501-21504 CD denotes 542
T31075 21504-21505 -RRB- denotes )
T31076 21505-21506 . denotes .
T31407 22531-22534 DT denotes The
T31408 22540-22547 NN denotes protein
T31409 22535-22537 NN denotes mr
T31410 22538-22539 NN denotes s
T31411 22537-22538 HYPH denotes -
T31412 22557-22566 VB denotes associate
T31413 22548-22551 MD denotes can
T31414 22552-22556 NN denotes self
T31415 22556-22557 HYPH denotes -
T31416 22567-22569 IN denotes in
T31417 22570-22579 JJ denotes mammalian
T31418 22580-22585 NNS denotes cells
T31419 22585-22586 . denotes .
T31420 22586-22662 sentence denotes (A) Schematic drawing of the constructs used for immunoprecipitation assay.
T31421 22587-22588 -LRB- denotes (
T31422 22588-22589 LS denotes A
T31423 22601-22608 NN denotes drawing
T31424 22589-22590 -RRB- denotes )
T31425 22591-22600 JJ denotes Schematic
T31426 22609-22611 IN denotes of
T31427 22612-22615 DT denotes the
T31428 22616-22626 NNS denotes constructs
T31429 22627-22631 VBN denotes used
T31430 22632-22635 IN denotes for
T31431 22636-22655 NN denotes immunoprecipitation
T31432 22656-22661 NN denotes assay
T31433 22661-22662 . denotes .
T31434 22662-22840 sentence denotes HA-tagged or Flag-tagged full-length (amino acids 1 to 542), ΔSAM (amino acids 1 to 400) and SAM (amino acids 401 to 542) regions were inserted into pcDNA3 vector, respectively.
T31435 22663-22665 NN denotes HA
T31436 22666-22672 VBN denotes tagged
T31437 22665-22666 HYPH denotes -
T31438 22785-22792 NNS denotes regions
T31439 22673-22675 CC denotes or
T31440 22676-22680 NN denotes Flag
T31441 22681-22687 VBN denotes tagged
T31442 22680-22681 HYPH denotes -
T31443 22688-22692 JJ denotes full
T31444 22693-22699 NN denotes length
T31445 22692-22693 HYPH denotes -
T31446 22700-22701 -LRB- denotes (
T31447 22713-22714 CD denotes 1
T31448 22701-22706 NN denotes amino
T31449 22707-22712 NNS denotes acids
T31450 22715-22717 IN denotes to
T31451 22718-22721 CD denotes 542
T31452 22721-22722 -RRB- denotes )
T31453 22722-22724 , denotes ,
T31454 22724-22728 NN denotes ΔSAM
T31455 22729-22730 -LRB- denotes (
T31456 22742-22743 CD denotes 1
T31457 22730-22735 NN denotes amino
T31458 22736-22741 NNS denotes acids
T31459 22744-22746 IN denotes to
T31460 22747-22750 CD denotes 400
T31461 22750-22751 -RRB- denotes )
T31462 22752-22755 CC denotes and
T31463 22756-22759 NN denotes SAM
T31464 22760-22761 -LRB- denotes (
T31465 22773-22776 CD denotes 401
T31466 22761-22766 NN denotes amino
T31467 22767-22772 NNS denotes acids
T31468 22777-22779 IN denotes to
T31469 22780-22783 CD denotes 542
T31470 22783-22784 -RRB- denotes )
T31471 22798-22806 VBN denotes inserted
T31472 22793-22797 VBD denotes were
T31473 22807-22811 IN denotes into
T31474 22812-22818 NN denotes pcDNA3
T31475 22819-22825 NN denotes vector
T31476 22825-22827 , denotes ,
T31477 22827-22839 RB denotes respectively
T31478 22839-22840 . denotes .
T31479 22840-22912 sentence denotes (B) The constructs indicated above were transfected into HEK293T cells.
T31480 22841-22842 -LRB- denotes (
T31481 22842-22843 LS denotes B
T31482 22881-22892 VBN denotes transfected
T31483 22843-22844 -RRB- denotes )
T31484 22845-22848 DT denotes The
T31485 22849-22859 NNS denotes constructs
T31486 22860-22869 VBN denotes indicated
T31487 22870-22875 RB denotes above
T31488 22876-22880 VBD denotes were
T31489 22893-22897 IN denotes into
T31490 22898-22905 NN denotes HEK293T
T31491 22906-22911 NNS denotes cells
T31492 22911-22912 . denotes .
T31493 22912-23004 sentence denotes Each lane was co-immunoprecipitated by anti-Flag antibody and detected by anti-HA antibody.
T31494 22913-22917 DT denotes Each
T31495 22918-22922 NN denotes lane
T31496 22927-22948 VBN denotes co-immunoprecipitated
T31497 22923-22926 VBD denotes was
T31498 22949-22951 IN denotes by
T31499 22952-22961 JJ denotes anti-Flag
T31500 22962-22970 NN denotes antibody
T31501 22971-22974 CC denotes and
T31502 22975-22983 VBN denotes detected
T31503 22984-22986 IN denotes by
T31504 22987-22994 JJ denotes anti-HA
T31505 22995-23003 NN denotes antibody
T31506 23003-23004 . denotes .
T31507 23004-23057 sentence denotes Input protein lysates are shown in the lower panels.
T31508 23005-23010 NN denotes Input
T31509 23019-23026 NNS denotes lysates
T31510 23011-23018 NN denotes protein
T31511 23031-23036 VBN denotes shown
T31512 23027-23030 VBP denotes are
T31513 23037-23039 IN denotes in
T31514 23040-23043 DT denotes the
T31515 23050-23056 NNS denotes panels
T31516 23044-23049 JJ denotes lower
T31517 23056-23057 . denotes .
T31518 23057-23179 sentence denotes (C) Flag-tagged two site-directed mr-s mutants, Flag-W404A and Flag-G453A were generated and co-transfected with full-HA.
T31519 23058-23059 -LRB- denotes (
T31520 23059-23060 LS denotes C
T31521 23137-23146 VBN denotes generated
T31522 23060-23061 -RRB- denotes )
T31523 23062-23066 NN denotes Flag
T31524 23067-23073 VBN denotes tagged
T31525 23066-23067 HYPH denotes -
T31526 23097-23104 NNS denotes mutants
T31527 23074-23077 CD denotes two
T31528 23078-23082 NN denotes site
T31529 23083-23091 VBN denotes directed
T31530 23082-23083 HYPH denotes -
T31531 23092-23094 NN denotes mr
T31532 23095-23096 NN denotes s
T31533 23094-23095 HYPH denotes -
T31534 23104-23106 , denotes ,
T31535 23106-23110 NN denotes Flag
T31536 23111-23116 NN denotes W404A
T31537 23110-23111 HYPH denotes -
T31538 23117-23120 CC denotes and
T31539 23121-23125 NN denotes Flag
T31540 23126-23131 NN denotes G453A
T31541 23125-23126 HYPH denotes -
T31542 23132-23136 VBD denotes were
T31543 23147-23150 CC denotes and
T31544 23151-23165 VBN denotes co-transfected
T31545 23166-23170 IN denotes with
T31546 23171-23175 JJ denotes full
T31547 23176-23178 NN denotes HA
T31548 23175-23176 HYPH denotes -
T31549 23178-23179 . denotes .
T31550 23179-23271 sentence denotes Each lane was co-immunoprecipitated by anti-HA antibody and detected by anti-Flag antibody.
T31551 23180-23184 DT denotes Each
T31552 23185-23189 NN denotes lane
T31553 23194-23215 VBN denotes co-immunoprecipitated
T31554 23190-23193 VBD denotes was
T31555 23216-23218 IN denotes by
T31556 23219-23226 JJ denotes anti-HA
T31557 23227-23235 NN denotes antibody
T31558 23236-23239 CC denotes and
T31559 23240-23248 VBN denotes detected
T31560 23249-23251 IN denotes by
T31561 23252-23261 JJ denotes anti-Flag
T31562 23262-23270 NN denotes antibody
T31563 23270-23271 . denotes .
T31729 25070-25081 JJ denotes Subcellular
T31730 25082-25094 NN denotes localization
T31731 25095-25097 IN denotes of
T31732 25098-25100 NN denotes mr
T31733 25101-25102 NN denotes s
T31734 25100-25101 HYPH denotes -
T31735 25103-25105 IN denotes in
T31736 25106-25115 JJ denotes mammalian
T31737 25116-25121 NNS denotes cells
T31738 25121-25122 . denotes .
T31739 25122-25227 sentence denotes (A, B) HA-tagged full-length mr-s was transfected into HEK293T (B) and detected by anti-HA antibody (A).
T31740 25123-25124 -LRB- denotes (
T31741 25124-25125 LS denotes A
T31742 25161-25172 VBN denotes transfected
T31743 25125-25127 , denotes ,
T31744 25127-25128 LS denotes B
T31745 25128-25129 -RRB- denotes )
T31746 25130-25132 NN denotes HA
T31747 25133-25139 VBN denotes tagged
T31748 25132-25133 HYPH denotes -
T31749 25155-25156 NN denotes s
T31750 25140-25144 JJ denotes full
T31751 25145-25151 NN denotes length
T31752 25144-25145 HYPH denotes -
T31753 25152-25154 NN denotes mr
T31754 25154-25155 HYPH denotes -
T31755 25157-25160 VBD denotes was
T31756 25173-25177 IN denotes into
T31757 25178-25185 NN denotes HEK293T
T31758 25186-25187 -LRB- denotes (
T31759 25187-25188 NN denotes B
T31760 25188-25189 -RRB- denotes )
T31761 25190-25193 CC denotes and
T31762 25194-25202 VBN denotes detected
T31763 25203-25205 IN denotes by
T31764 25206-25213 JJ denotes anti-HA
T31765 25214-25222 NN denotes antibody
T31766 25223-25224 -LRB- denotes (
T31767 25224-25225 NN denotes A
T31768 25225-25226 -RRB- denotes )
T31769 25226-25227 . denotes .
T31770 25227-25245 sentence denotes Scale bar, 20 μm.
T31771 25228-25233 NN denotes Scale
T31772 25234-25237 NN denotes bar
T31773 25237-25239 , denotes ,
T31774 25239-25241 CD denotes 20
T31775 25242-25244 NN denotes μm
T31776 25244-25245 . denotes .
T31777 25245-25338 sentence denotes (C-E) HEK293T cells immunostained with anti-HA antibody (C), DAPI (D), and merged image (E).
T31778 25246-25247 -LRB- denotes (
T31779 25247-25248 LS denotes C
T31780 25260-25265 NNS denotes cells
T31781 25248-25249 SYM denotes -
T31782 25249-25250 LS denotes E
T31783 25250-25251 -RRB- denotes )
T31784 25252-25259 NN denotes HEK293T
T31785 25266-25279 VBN denotes immunostained
T31786 25280-25284 IN denotes with
T31787 25285-25292 JJ denotes anti-HA
T31788 25293-25301 NN denotes antibody
T31789 25302-25303 -LRB- denotes (
T31790 25303-25304 NN denotes C
T31791 25304-25305 -RRB- denotes )
T31792 25305-25307 , denotes ,
T31793 25307-25311 NN denotes DAPI
T31794 25312-25313 -LRB- denotes (
T31795 25313-25314 NN denotes D
T31796 25314-25315 -RRB- denotes )
T31797 25315-25317 , denotes ,
T31798 25317-25320 CC denotes and
T31799 25321-25327 VBN denotes merged
T31800 25328-25333 NN denotes image
T31801 25334-25335 -LRB- denotes (
T31802 25335-25336 NN denotes E
T31803 25336-25337 -RRB- denotes )
T31804 25337-25338 . denotes .
T31805 25338-25356 sentence denotes Scale bar, 50 μm.
T31806 25339-25344 NN denotes Scale
T31807 25345-25348 NN denotes bar
T31808 25348-25350 , denotes ,
T31809 25350-25352 CD denotes 50
T31810 25353-25355 NN denotes μm
T31811 25355-25356 . denotes .
T32444 29763-29765 NN denotes mr
T32445 29766-29767 NN denotes s
T32446 29765-29766 HYPH denotes -
T32447 29801-29810 VBZ denotes functions
T32448 29768-29773 VBN denotes fused
T32449 29774-29776 IN denotes to
T32450 29777-29781 NN denotes GAL4
T32451 29794-29800 NN denotes domain
T32452 29782-29785 NN denotes DNA
T32453 29786-29793 VBG denotes binding
T32454 29811-29813 IN denotes as
T32455 29814-29815 DT denotes a
T32456 29832-29841 NN denotes repressor
T32457 29816-29831 JJ denotes transcriptional
T32458 29842-29844 IN denotes in
T32459 29845-29852 NN denotes HEK293T
T32460 29853-29858 NNS denotes cells
T32461 29858-29859 . denotes .
T32462 29859-29930 sentence denotes (A) Schematic drawing of the constructs used for the luciferase assay.
T32463 29860-29861 -LRB- denotes (
T32464 29861-29862 LS denotes A
T32465 29874-29881 NN denotes drawing
T32466 29862-29863 -RRB- denotes )
T32467 29864-29873 JJ denotes Schematic
T32468 29882-29884 IN denotes of
T32469 29885-29888 DT denotes the
T32470 29889-29899 NNS denotes constructs
T32471 29900-29904 VBN denotes used
T32472 29905-29908 IN denotes for
T32473 29909-29912 DT denotes the
T32474 29924-29929 NN denotes assay
T32475 29913-29923 NN denotes luciferase
T32476 29929-29930 . denotes .
T32477 29930-30075 sentence denotes 5xGAL4-pGL3 reporter plasmid was co-transfected into HEK293T cells with effector plasmids expressing various deletion mutants fused to GAL4-DBD.
T32478 29931-29937 NN denotes 5xGAL4
T32479 29938-29942 NN denotes pGL3
T32480 29937-29938 HYPH denotes -
T32481 29952-29959 NN denotes plasmid
T32482 29943-29951 NN denotes reporter
T32483 29964-29978 VBN denotes co-transfected
T32484 29960-29963 VBD denotes was
T32485 29979-29983 IN denotes into
T32486 29984-29991 NN denotes HEK293T
T32487 29992-29997 NNS denotes cells
T32488 29998-30002 IN denotes with
T32489 30003-30011 NN denotes effector
T32490 30012-30020 NNS denotes plasmids
T32491 30021-30031 VBG denotes expressing
T32492 30032-30039 JJ denotes various
T32493 30049-30056 NNS denotes mutants
T32494 30040-30048 NN denotes deletion
T32495 30057-30062 VBN denotes fused
T32496 30063-30065 IN denotes to
T32497 30066-30070 NN denotes GAL4
T32498 30071-30074 NN denotes DBD
T32499 30070-30071 HYPH denotes -
T32500 30074-30075 . denotes .
T32501 30075-30198 sentence denotes (B) Various amounts of DBD, DBD-mrs, DBD-N or DBD-C plasmids were transfected with 0.1 μg of 5xGAL4-pGL3 reporter plasmid.
T32502 30076-30077 -LRB- denotes (
T32503 30077-30078 LS denotes B
T32504 30142-30153 VBN denotes transfected
T32505 30078-30079 -RRB- denotes )
T32506 30080-30087 JJ denotes Various
T32507 30088-30095 NNS denotes amounts
T32508 30096-30098 IN denotes of
T32509 30099-30102 NN denotes DBD
T32510 30128-30136 NNS denotes plasmids
T32511 30102-30104 , denotes ,
T32512 30104-30107 NN denotes DBD
T32513 30108-30111 NN denotes mrs
T32514 30107-30108 HYPH denotes -
T32515 30111-30113 , denotes ,
T32516 30113-30116 NN denotes DBD
T32517 30117-30118 NN denotes N
T32518 30116-30117 HYPH denotes -
T32519 30119-30121 CC denotes or
T32520 30122-30125 NN denotes DBD
T32521 30126-30127 NN denotes C
T32522 30125-30126 HYPH denotes -
T32523 30137-30141 VBD denotes were
T32524 30154-30158 IN denotes with
T32525 30159-30162 CD denotes 0.1
T32526 30163-30165 NN denotes μg
T32527 30166-30168 IN denotes of
T32528 30169-30175 NN denotes 5xGAL4
T32529 30176-30180 NN denotes pGL3
T32530 30175-30176 HYPH denotes -
T32531 30190-30197 NN denotes plasmid
T32532 30181-30189 NN denotes reporter
T32533 30197-30198 . denotes .
T32534 30198-30287 sentence denotes The reporter activity in the presence of the pcDNA3 vector (pcDNA3) was designated as 1.
T32535 30199-30202 DT denotes The
T32536 30212-30220 NN denotes activity
T32537 30203-30211 NN denotes reporter
T32538 30271-30281 VBN denotes designated
T32539 30221-30223 IN denotes in
T32540 30224-30227 DT denotes the
T32541 30228-30236 NN denotes presence
T32542 30237-30239 IN denotes of
T32543 30240-30243 DT denotes the
T32544 30251-30257 NN denotes vector
T32545 30244-30250 NN denotes pcDNA3
T32546 30258-30259 -LRB- denotes (
T32547 30259-30265 NN denotes pcDNA3
T32548 30265-30266 -RRB- denotes )
T32549 30267-30270 VBD denotes was
T32550 30282-30284 IN denotes as
T32551 30285-30286 CD denotes 1
T32552 30286-30287 . denotes .
T32553 30287-30332 sentence denotes Error bars represent standard error of mean.
T32554 30288-30293 NN denotes Error
T32555 30294-30298 NNS denotes bars
T32556 30299-30308 VBP denotes represent
T32557 30309-30317 JJ denotes standard
T32558 30318-30323 NN denotes error
T32559 30324-30326 IN denotes of
T32560 30327-30331 NN denotes mean
T32561 30331-30332 . denotes .
T32562 30332-30434 sentence denotes (C) DBD-W404A and DBD-G453A were co-transfected into HEK293T cells with 5xGAL4-pGL3 reporter plasmid.
T32563 30333-30334 -LRB- denotes (
T32564 30334-30335 LS denotes C
T32565 30366-30380 VBN denotes co-transfected
T32566 30335-30336 -RRB- denotes )
T32567 30337-30340 NN denotes DBD
T32568 30341-30346 NN denotes W404A
T32569 30340-30341 HYPH denotes -
T32570 30347-30350 CC denotes and
T32571 30351-30354 NN denotes DBD
T32572 30355-30360 NN denotes G453A
T32573 30354-30355 HYPH denotes -
T32574 30361-30365 VBD denotes were
T32575 30381-30385 IN denotes into
T32576 30386-30393 NN denotes HEK293T
T32577 30394-30399 NNS denotes cells
T32578 30400-30404 IN denotes with
T32579 30405-30411 NN denotes 5xGAL4
T32580 30412-30416 NN denotes pGL3
T32581 30411-30412 HYPH denotes -
T32582 30426-30433 NN denotes plasmid
T32583 30417-30425 NN denotes reporter
T32584 30433-30434 . denotes .
T32585 30434-30532 sentence denotes Fold repression was calculated as the fold decrease in luciferase activity compared with DBD-mrs.
T32586 30435-30439 NN denotes Fold
T32587 30440-30450 NN denotes repression
T32588 30455-30465 VBN denotes calculated
T32589 30451-30454 VBD denotes was
T32590 30466-30468 IN denotes as
T32591 30469-30472 DT denotes the
T32592 30478-30486 NN denotes decrease
T32593 30473-30477 NN denotes fold
T32594 30487-30489 IN denotes in
T32595 30490-30500 NN denotes luciferase
T32596 30501-30509 NN denotes activity
T32597 30510-30518 VBN denotes compared
T32598 30519-30523 IN denotes with
T32599 30524-30527 NN denotes DBD
T32600 30528-30531 NN denotes mrs
T32601 30527-30528 HYPH denotes -
T32602 30531-30532 . denotes .
T32603 30532-30573 sentence denotes Error bars represent standard deviation.
T32604 30533-30538 NN denotes Error
T32605 30539-30543 NNS denotes bars
T32606 30544-30553 VBP denotes represent
T32607 30554-30562 JJ denotes standard
T32608 30563-30572 NN denotes deviation
T32609 30572-30573 . denotes .
T32610 30573-30668 sentence denotes (D) Various amounts of DBD-tail or DBD-SAM were transfected with 5xGAL4-pGL3 reporter plasmid.
T32611 30574-30575 -LRB- denotes (
T32612 30575-30576 LS denotes D
T32613 30622-30633 VBN denotes transfected
T32614 30576-30577 -RRB- denotes )
T32615 30578-30585 JJ denotes Various
T32616 30586-30593 NNS denotes amounts
T32617 30594-30596 IN denotes of
T32618 30597-30600 NN denotes DBD
T32619 30601-30605 NN denotes tail
T32620 30600-30601 HYPH denotes -
T32621 30606-30608 CC denotes or
T32622 30609-30612 NN denotes DBD
T32623 30613-30616 NN denotes SAM
T32624 30612-30613 HYPH denotes -
T32625 30617-30621 VBD denotes were
T32626 30634-30638 IN denotes with
T32627 30639-30645 NN denotes 5xGAL4
T32628 30646-30650 NN denotes pGL3
T32629 30645-30646 HYPH denotes -
T32630 30660-30667 NN denotes plasmid
T32631 30651-30659 NN denotes reporter
T32632 30667-30668 . denotes .
T32633 30668-30713 sentence denotes Error bars represent standard error of mean.
T32634 30669-30674 NN denotes Error
T32635 30675-30679 NNS denotes bars
T32636 30680-30689 VBP denotes represent
T32637 30690-30698 JJ denotes standard
T32638 30699-30704 NN denotes error
T32639 30705-30707 IN denotes of
T32640 30708-30712 NN denotes mean
T32641 30712-30713 . denotes .
T32642 30713-30836 sentence denotes (E) pcDNA3 or DBD-mrs (5 μg) was co-transfected into Y79 retinoblastoma cells with 0.5 μg of 5xGAL4-pGL3 reporter plasmid.
T32643 30714-30715 -LRB- denotes (
T32644 30715-30716 LS denotes E
T32645 30747-30761 VBN denotes co-transfected
T32646 30716-30717 -RRB- denotes )
T32647 30718-30724 NN denotes pcDNA3
T32648 30725-30727 CC denotes or
T32649 30728-30731 NN denotes DBD
T32650 30732-30735 NN denotes mrs
T32651 30731-30732 HYPH denotes -
T32652 30736-30737 -LRB- denotes (
T32653 30739-30741 NN denotes μg
T32654 30737-30738 CD denotes 5
T32655 30741-30742 -RRB- denotes )
T32656 30743-30746 VBD denotes was
T32657 30762-30766 IN denotes into
T32658 30767-30770 NN denotes Y79
T32659 30786-30791 NNS denotes cells
T32660 30771-30785 NN denotes retinoblastoma
T32661 30792-30796 IN denotes with
T32662 30797-30800 CD denotes 0.5
T32663 30801-30803 NN denotes μg
T32664 30804-30806 IN denotes of
T32665 30807-30813 NN denotes 5xGAL4
T32666 30814-30818 NN denotes pGL3
T32667 30813-30814 HYPH denotes -
T32668 30828-30835 NN denotes plasmid
T32669 30819-30827 NN denotes reporter
T32670 30835-30836 . denotes .
T32671 30836-30905 sentence denotes The reporter activity in the presence of pcDNA3 was designated as 1.
T32672 30837-30840 DT denotes The
T32673 30850-30858 NN denotes activity
T32674 30841-30849 NN denotes reporter
T32675 30889-30899 VBN denotes designated
T32676 30859-30861 IN denotes in
T32677 30862-30865 DT denotes the
T32678 30866-30874 NN denotes presence
T32679 30875-30877 IN denotes of
T32680 30878-30884 NN denotes pcDNA3
T32681 30885-30888 VBD denotes was
T32682 30900-30902 IN denotes as
T32683 30903-30904 CD denotes 1
T32684 30904-30905 . denotes .
T32685 30905-30946 sentence denotes Error bars represent standard deviation.
T32686 30906-30911 NN denotes Error
T32687 30912-30916 NNS denotes bars
T32688 30917-30926 VBP denotes represent
T32689 30927-30935 JJ denotes standard
T32690 30936-30945 NN denotes deviation
T32691 30945-30946 . denotes .
T32692 30946-31028 sentence denotes Asterisk marks statistically significant difference (Student's t test: p < 0.03).
T32693 30947-30955 NN denotes Asterisk
T32694 30956-30961 VBZ denotes marks
T32695 30962-30975 RB denotes statistically
T32696 30976-30987 JJ denotes significant
T32697 30988-30998 NN denotes difference
T32698 30999-31000 -LRB- denotes (
T32699 31012-31016 NN denotes test
T32700 31000-31007 NNP denotes Student
T32701 31007-31009 POS denotes 's
T32702 31010-31011 NN denotes t
T32703 31016-31018 : denotes :
T32704 31018-31019 NN denotes p
T32705 31022-31026 CD denotes 0.03
T32706 31020-31021 SYM denotes <
T32707 31026-31027 -RRB- denotes )
T32708 31027-31028 . denotes .
T32709 31028-31126 sentence denotes (F) Alignment of the C-terminal regions for mouse, rat, human, chick and zebrafish mr-s proteins.
T32710 31029-31030 -LRB- denotes (
T32711 31030-31031 LS denotes F
T32712 31033-31042 NN denotes Alignment
T32713 31031-31032 -RRB- denotes )
T32714 31043-31045 IN denotes of
T32715 31046-31049 DT denotes the
T32716 31061-31068 NNS denotes regions
T32717 31050-31051 NN denotes C
T32718 31052-31060 JJ denotes terminal
T32719 31051-31052 HYPH denotes -
T32720 31069-31072 IN denotes for
T32721 31073-31078 NN denotes mouse
T32722 31117-31125 NN denotes proteins
T32723 31078-31080 , denotes ,
T32724 31080-31083 NN denotes rat
T32725 31083-31085 , denotes ,
T32726 31085-31090 JJ denotes human
T32727 31090-31092 , denotes ,
T32728 31092-31097 NN denotes chick
T32729 31098-31101 CC denotes and
T32730 31102-31111 NN denotes zebrafish
T32731 31112-31114 NN denotes mr
T32732 31115-31116 NN denotes s
T32733 31114-31115 HYPH denotes -
T32734 31125-31126 . denotes .
T32735 31126-31186 sentence denotes Conserved amino acid residues are shown with a dark shadow.
T32736 31127-31136 VBN denotes Conserved
T32737 31148-31156 NNS denotes residues
T32738 31137-31142 NN denotes amino
T32739 31143-31147 NN denotes acid
T32740 31161-31166 VBN denotes shown
T32741 31157-31160 VBP denotes are
T32742 31167-31171 IN denotes with
T32743 31172-31173 DT denotes a
T32744 31179-31185 NN denotes shadow
T32745 31174-31178 JJ denotes dark
T32746 31185-31186 . denotes .
T7642 12426-12427 NN denotes c
T7643 12427-12430 sentence denotes ifi
T7644 12427-12428 NN denotes i
T7645 12428-12429 IN denotes f
T7646 12429-12430 NN denotes i
T7653 12438-12439 IN denotes m
T7654 12439-12440 NN denotes r
T7655 12440-12561 sentence denotes -s expression, the expression of the mr-s gene in various adult tissues was examined by Northern hybridization (Fig. 2H).
T7656 12516-12524 VBN denotes examined
T7657 12441-12442 NN denotes s
T7658 12440-12441 HYPH denotes -
T7659 12443-12453 NN denotes expression
T7660 12453-12455 , denotes ,
T7661 12455-12458 DT denotes the
T9937 14898-14900 NN denotes mr
T9938 14901-14902 NN denotes s
T1003 0-7 NN denotes Cloning
T9939 14900-14901 HYPH denotes -
T7556 11156-11163 IN denotes between
T7557 11164-11166 NN denotes P6
T7558 11166-11167 SYM denotes -
T7559 11167-11169 NN denotes P8
T7560 11170-11171 -LRB- denotes [
T7561 11171-11173 CD denotes 34
T7562 11173-11174 SYM denotes -
T7563 11174-11176 CD denotes 37
T7564 11176-11177 -RRB- denotes ]
T7565 11177-11178 . denotes .
T7566 11178-11313 sentence denotes At P9, the intensity of the mr-s signal was significantly reduced but was localized to the outer nuclear layer (ONL) (data not shown).
T7567 11179-11181 IN denotes At
T7568 11237-11244 VBN denotes reduced
T7569 11182-11184 NN denotes P9
T7570 11184-11186 , denotes ,
T7571 11186-11189 DT denotes the
T7572 11190-11199 NN denotes intensity
T7573 11200-11202 IN denotes of
T7574 11203-11206 DT denotes the
T7575 11212-11218 NN denotes signal
T7576 11207-11209 NN denotes mr
T7577 11210-11211 NN denotes s
T7578 11209-11210 HYPH denotes -
T7579 11219-11222 VBD denotes was
T7580 11223-11236 RB denotes significantly
T7581 11245-11248 CC denotes but
T7582 11249-11252 VBD denotes was
T7583 11253-11262 VBN denotes localized
T7584 11263-11265 IN denotes to
T7585 11266-11269 DT denotes the
T7586 11284-11289 NN denotes layer
T7587 11270-11275 JJ denotes outer
T7588 11276-11283 JJ denotes nuclear
T7589 11290-11291 -LRB- denotes (
T7625 12406-12407 sentence denotes r
T7626 12406-12407 NN denotes r
T7627 12407-12423 sentence denotes mine the tissue
T7628 12408-12410 NN denotes in
T7629 12407-12408 NN denotes m
T7630 12410-12411 VBN denotes e
T7631 12412-12413 JJ denotes t
T7632 12413-12415 DT denotes he
T7633 12416-12418 NN denotes ti
T7634 12419-12420 VBD denotes s
T7635 12418-12419 NN denotes s
T7636 12421-12422 NN denotes e
T7637 12420-12421 JJ denotes u
T7638 12423-12426 sentence denotes spe
T7639 12424-12426 NN denotes pe
T7640 12423-12424 NN denotes s
T7641 12426-12427 sentence denotes c
T7647 12430-12433 sentence denotes cit
T7648 12430-12431 VBZ denotes c
T7649 12431-12433 NN denotes it
T7650 12433-12440 sentence denotes y of mr
T7651 12433-12434 NN denotes y
T7652 12435-12437 IN denotes of
T7863 13368-13371 CD denotes 452
T9940 14903-14916 NN denotes transcription
T9941 14917-14919 VBZ denotes is
T9915 14778-14780 NN denotes mr
T9916 14781-14782 NN denotes s
T9917 14780-14781 HYPH denotes -
T9918 14783-14796 NN denotes transcription
T9919 14797-14800 VBD denotes was
T9920 14801-14814 RB denotes significantly
T9921 14829-14831 IN denotes in
T9922 14832-14835 DT denotes the
T9923 14850-14855 NN denotes gland
T9924 14836-14839 NN denotes Crx
T9925 14840-14842 NN denotes KO
T9926 14843-14849 JJ denotes pineal
T9927 14855-14856 . denotes .
T9928 14856-14987 sentence denotes Taken together, these data indicate that mr-s transcription is regulated by Crx in the developing photoreceptors and pineal gland.
T9929 14857-14862 VBN denotes Taken
T9930 14884-14892 VBP denotes indicate
T9931 14863-14871 RB denotes together
T9932 14871-14873 , denotes ,
T9933 14873-14878 DT denotes these
T9934 14879-14883 NNS denotes data
T9935 14893-14897 IN denotes that
T9936 14920-14929 VBN denotes regulated
T9942 14930-14932 IN denotes by
T9943 14933-14936 NN denotes Crx
T9944 14937-14939 IN denotes in
T9945 14940-14943 DT denotes the
T9946 14955-14969 NNS denotes photoreceptors
T9947 14944-14954 VBG denotes developing
T9948 14970-14973 CC denotes and
T9949 14974-14980 JJ denotes pineal
T9950 14981-14986 NN denotes gland
T9951 14986-14987 . denotes .
T9952 14987-14988 sentence denotes
T9953 16029-16035 sentence denotes To fur
T9954 16029-16030 DT denotes T
T9955 16030-16031 NN denotes o
T9956 16033-16034 VBN denotes u
T9957 16032-16033 IN denotes f
T9958 16034-16035 NN denotes r
T9959 16035-16043 sentence denotes ther exa
T9960 16035-16036 FW denotes t
T9961 16036-16037 NN denotes h
T9971 16048-16049 sentence denotes w
T9972 16048-16049 JJ denotes w
T9973 16049-16056 sentence denotes hether
T9974 16049-16051 DT denotes he
T9975 16051-16052 NNS denotes t
T9976 16052-16054 DT denotes he
T9977 16054-16055 NN denotes r
T9978 16056-16058 sentence denotes Cr
T9979 16057-16058 NN denotes r
T9980 16056-16057 NN denotes C
T9981 16058-16062 sentence denotes x re
T9982 16060-16062 VBZ denotes re
T9983 16058-16059 NN denotes x
T9984 16062-16063 sentence denotes g
T9985 16062-16063 NN denotes g
T9986 16063-16068 sentence denotes ulate
T9987 16063-16064 NN denotes u
T9988 16064-16065 NN denotes l
T9989 16065-16066 CC denotes a
T9990 16066-16068 RB denotes te
T9991 16068-16077 sentence denotes s mr-s tr
T9992 16068-16069 VBZ denotes s
T9993 16072-16074 VBN denotes -s
T9994 16070-16071 NN denotes m
T9995 16071-16072 VBD denotes r
T9996 16075-16076 IN denotes t
T9997 16076-16077 NN denotes r
T9998 16077-16080 sentence denotes ans
T9999 16077-16078 NNS denotes a
T10000 16078-16079 VBP denotes n
T10001 16079-16080 JJ denotes s
T10002 16080-16320 sentence denotes cription directly or not, we next performed a luciferase assay using the 1.2-kb proximal promoter region of mr-s fused to a luciferase gene as a luciferase reporter (Fig. 3D, Pro1.2k) and the Crx, Otx2, Nrl expression vectors, respectively.
T10003 16114-16123 VBD denotes performed
T10004 16080-16088 NN denotes cription
T10005 16089-16097 RB denotes directly
T10006 16098-16100 CC denotes or
T10007 16101-16104 RB denotes not
T10008 16104-16106 , denotes ,
T10009 16106-16108 PRP denotes we
T10010 16109-16113 RB denotes next
T10011 16124-16125 DT denotes a
T10012 16137-16142 NN denotes assay
T10013 16126-16136 NN denotes luciferase
T10014 16143-16148 VBG denotes using
T10015 16149-16152 DT denotes the
T10016 16178-16184 NN denotes region
T10017 16153-16156 CD denotes 1.2
T10018 16157-16159 NN denotes kb
T10019 16156-16157 HYPH denotes -
T13281 23429-23433 NN denotes Fig.
T13282 23434-23436 NN denotes 1B
T12794 19822-19826 VBZ denotes does
T13283 23436-23438 , denotes ,
T12899 20274-20275 HYPH denotes -
T12900 20277-20278 -LRB- denotes (
T12901 20294-20295 NN denotes C
T12902 20278-20282 NN denotes Fig.
T12903 20283-20285 NN denotes 4B
T12904 20285-20287 , denotes ,
T12998 21507-21753 sentence denotes To confirm self-association of the mr-s protein in mammalian cells, we next performed co-immunoprecipitation studies in HEK293T cells by co-transfection of HA-tagged full-length/truncated mr-s and Flag-tagged full-length/truncated mr-s (Fig. 5A).
T12999 21507-21509 TO denotes To
T13000 21510-21517 VB denotes confirm
T13001 21583-21592 VBD denotes performed
T13002 21518-21522 NN denotes self
T13003 21523-21534 NN denotes association
T13004 21522-21523 HYPH denotes -
T13005 21535-21537 IN denotes of
T13006 21538-21541 DT denotes the
T13007 21547-21554 NN denotes protein
T13008 21542-21544 NN denotes mr
T13009 21545-21546 NN denotes s
T13010 21544-21545 HYPH denotes -
T13011 21555-21557 IN denotes in
T13012 21558-21567 JJ denotes mammalian
T13013 21568-21573 NNS denotes cells
T13014 21573-21575 , denotes ,
T13015 21575-21577 PRP denotes we
T13016 21578-21582 RB denotes next
T13017 21593-21615 NN denotes co-immunoprecipitation
T13018 21616-21623 NNS denotes studies
T13019 21624-21626 IN denotes in
T13020 21627-21634 NN denotes HEK293T
T13021 21635-21640 NNS denotes cells
T13022 21641-21643 IN denotes by
T13023 21644-21659 NN denotes co-transfection
T13024 21660-21662 IN denotes of
T13025 21663-21665 NN denotes HA
T13026 21666-21672 VBN denotes tagged
T13236 22497-22500 NN denotes SAM
T13237 22496-22497 HYPH denotes -
T13238 22501-22502 -LRB- denotes (
T13239 22507-22509 NN denotes 5B
T13240 22502-22506 NN denotes Fig.
T13241 22509-22511 , denotes ,
T13242 22511-22515 NN denotes lane
T13243 22516-22518 CD denotes 10
T13244 22518-22519 -RRB- denotes )
T13245 22519-22520 . denotes .
T13246 22520-23446 sentence denotes Figure 5 The mr-s protein can self-associate in mammalian cells. (A) Schematic drawing of the constructs used for immunoprecipitation assay. HA-tagged or Flag-tagged full-length (amino acids 1 to 542), ΔSAM (amino acids 1 to 400) and SAM (amino acids 401 to 542) regions were inserted into pcDNA3 vector, respectively. (B) The constructs indicated above were transfected into HEK293T cells. Each lane was co-immunoprecipitated by anti-Flag antibody and detected by anti-HA antibody. Input protein lysates are shown in the lower panels. (C) Flag-tagged two site-directed mr-s mutants, Flag-W404A and Flag-G453A were generated and co-transfected with full-HA. Each lane was co-immunoprecipitated by anti-HA antibody and detected by anti-Flag antibody. To investigate whether the mr-s protein self-associates mainly through the SAM domain, two site-directed mutations were generated in the SAM domain of mr-s (Fig. 1B, arrows).
T13247 23272-23274 TO denotes To
T13248 23275-23286 VB denotes investigate
T13249 23392-23401 VBN denotes generated
T13250 23287-23294 IN denotes whether
T13251 23317-23327 VBZ denotes associates
T13252 23295-23298 DT denotes the
T13253 23304-23311 NN denotes protein
T13254 23299-23301 NN denotes mr
T13255 23302-23303 NN denotes s
T13256 23301-23302 HYPH denotes -
T13257 23312-23316 NN denotes self
T13258 23316-23317 HYPH denotes -
T13259 23328-23334 RB denotes mainly
T13260 23335-23342 IN denotes through
T13261 23343-23346 DT denotes the
T13262 23351-23357 NN denotes domain
T13263 23347-23350 NN denotes SAM
T13264 23357-23359 , denotes ,
T13265 23359-23362 CD denotes two
T13266 23377-23386 NNS denotes mutations
T13267 23363-23367 NN denotes site
T13268 23368-23376 VBN denotes directed
T13269 23367-23368 HYPH denotes -
T13270 23387-23391 VBD denotes were
T13271 23402-23404 IN denotes in
T13272 23405-23408 DT denotes the
T13273 23413-23419 NN denotes domain
T13274 23409-23412 NN denotes SAM
T13275 23420-23422 IN denotes of
T13276 23423-23425 NN denotes mr
T13277 23426-23427 NN denotes s
T13278 23425-23426 HYPH denotes -
T13279 23428-23429 -LRB- denotes (
T13280 23438-23444 NNS denotes arrows
T16941 29584-29587 RB denotes not
T16942 29596-29610 JJ denotes characteristic
T16943 29611-29616 NN denotes amino
T16944 29617-29621 NN denotes acid
T16945 29622-29628 NNS denotes motifs
T16946 29629-29632 CC denotes and
T16947 29633-29636 DT denotes the
T16948 29637-29646 NN denotes mechanism
T16949 29714-29721 VBZ denotes remains
T16950 29647-29654 IN denotes through
T16951 29672-29680 VBZ denotes achieves
T16952 29655-29660 WDT denotes which
T16953 29661-29664 DT denotes the
T16954 29665-29671 NN denotes region
T16955 29681-29684 CC denotes and
T16956 29684-29685 HYPH denotes /
T16957 29685-29687 CC denotes or
T16958 29688-29697 VBZ denotes maintains
T16959 29698-29702 NN denotes gene
T16960 29703-29713 NN denotes repression
T16961 29722-29724 TO denotes to
T16962 29728-29737 VBN denotes clarified
T16963 29725-29727 VB denotes be
T16964 29738-29740 IN denotes in
T16965 29741-29744 DT denotes the
T16966 29745-29751 NN denotes future
T16967 29751-29752 . denotes .
T3593 2248-2250 IN denotes In
T3594 2325-2334 VBN denotes generated
T3595 2251-2254 DT denotes the
T3596 2255-2266 NN denotes development
T3597 2267-2269 IN denotes of
T3598 2270-2273 DT denotes the
T3599 2284-2290 NN denotes retina
T3600 2274-2283 JJ denotes mammalian
T3601 2290-2292 , denotes ,
T3602 2292-2293 DT denotes a
T3603 2302-2307 NN denotes range
T3604 2294-2301 JJ denotes diverse
T3605 2308-2310 IN denotes of
T3606 2311-2315 NN denotes cell
T3607 2316-2321 NNS denotes types
T3608 2322-2324 VBZ denotes is
T3609 2335-2339 IN denotes from
T3610 2340-2341 DT denotes a
R5 T1009 T1007 pobj s,of
R6 T1010 T1009 punct -,s
R9 T1013 T1009 appos protein,s
R10 T1014 T1013 amod novel,protein
R11 T1015 T1016 compound SAM,domain
R12 T1016 T1013 compound domain,protein
R18 T1022 T1020 pobj cells,in
R19 T1023 T1022 compound photoreceptor,cells
R21 T1029 T1031 nmod motif,domains
R22 T1030 T1029 nmod alpha,motif
R23 T1031 T1035 nsubj domains,are
R24 T1032 T1029 punct (,motif
R25 T1033 T1029 appos SAM,motif
R26 T1034 T1031 punct ),domains
R34 T1043 T1042 auxpass been,reported
R37 T1046 T1044 pobj modules,as
R38 T1047 T1048 compound protein,protein
R40 T1049 T1048 punct -,protein
R41 T1050 T1046 compound interaction,modules
R48 T1059 T1057 pobj number,in
R49 T1060 T1059 amod large,number
R56 T1067 T1064 pobj proteins,including
R57 T1068 T1066 punct (,group
R58 T1069 T1066 appos PcG,group
R59 T1070 T1067 punct ),proteins
R63 T1074 T1067 conj factors,proteins
R64 T1075 T1074 compound transcription,factors
R78 T1091 T1089 pobj protein,of
R79 T1092 T1091 amod novel,protein
R80 T1093 T1094 compound SAM,domain
R81 T1094 T1095 npadvmod domain,containing
R83 T1096 T1095 punct -,containing
R86 T1099 T1091 relcl expressed,protein
R87 T1100 T1099 auxpass is,expressed
R88 T1101 T1099 advmod predominantly,expressed
R94 T1107 T1104 conj gland,photoreceptors
R95 T1108 T1107 amod pineal,gland
R100 T1113 T1111 oprd s,designated
R101 T1114 T1113 compound mr,s
R102 T1115 T1113 punct -,s
R104 T1117 T1113 parataxis protein,s
R105 T1118 T1117 amod major,protein
R106 T1119 T1117 amod retinal,protein
R107 T1120 T1121 compound SAM,domain
R108 T1121 T1117 compound domain,protein
R112 T1128 T1130 nsubjpass s,conserved
R113 T1129 T1128 punct -,s
R123 T1140 T1138 relcl conserved,organisms
R124 T1141 T1139 pobj which,through
R125 T1142 T1143 det the,mechanism
R126 T1143 T1140 nsubjpass mechanism,conserved
R127 T1144 T1143 prep of,mechanism
R128 T1145 T1146 compound photoreceptor,development
R129 T1146 T1144 pobj development,of
R130 T1147 T1140 auxpass is,conserved
R131 T1148 T1140 advmod also,conserved
R132 T1149 T1140 advmod highly,conserved
R137 T1156 T1154 ccomp related,suggests
R138 T1157 T1158 det the,domain
R140 T1159 T1158 compound SAM,domain
R141 T1160 T1158 prep of,domain
R142 T1161 T1162 compound mr,s
R144 T1163 T1162 punct -,s
R145 T1164 T1156 auxpass is,related
R146 T1165 T1166 advmod most,closely
R147 T1166 T1156 advmod closely,related
R150 T1169 T1167 pobj ortholog,to
R151 T1170 T1169 nmod mouse,ortholog
R152 T1171 T1169 nmod polyhomeotic,ortholog
R153 T1172 T1173 punct (,ph
R154 T1173 T1171 parataxis ph,polyhomeotic
R155 T1174 T1173 punct ),ph
R158 T1177 T1169 appos Rae28,ortholog
R159 T1178 T1177 punct /,Rae28
R162 T1181 T1169 relcl known,ortholog
R163 T1182 T1181 auxpass is,known
R166 T1185 T1183 pobj molecule,as
R167 T1186 T1185 amod epigenetic,molecule
R178 T1199 T1197 acl play,possibility
R179 T1200 T1201 compound mr,s
R181 T1202 T1201 punct -,s
R182 T1203 T1199 aux may,play
R184 T1205 T1199 dobj role,play
R185 T1206 T1205 amod critical,role
R195 T1217 T1219 nsubjpass s,expressed
R196 T1218 T1217 punct -,s
R204 T1227 T1225 pobj 3,at
R205 T1228 T1227 nmod day,3
R215 T1238 T1227 advcl undergo,3
R216 T1239 T1238 nsubj photoreceptors,undergo
R223 T1246 T1244 pobj gland,in
R224 T1247 T1246 amod adult,gland
R225 T1248 T1246 amod pineal,gland
R230 T1255 T1253 pobj s,of
R231 T1256 T1255 punct -,s
R236 T1261 T1259 pobj protein,by
R237 T1262 T1263 compound cone,rod
R239 T1264 T1263 punct -,rod
R240 T1265 T1261 compound homeodomain,protein
R246 T1273 T1271 ccomp associates,showed
R247 T1274 T1275 det the,protein
R248 T1275 T1273 nsubj protein,associates
R249 T1276 T1277 compound mr,s
R250 T1277 T1275 compound s,protein
R251 T1278 T1277 punct -,s
R252 T1279 T1273 dep self,associates
R253 T1280 T1273 punct -,associates
R257 T1284 T1282 pobj region,through
R258 T1285 T1286 compound SAM,domain
R259 T1286 T1287 npadvmod domain,containing
R261 T1288 T1287 punct -,containing
R263 T1290 T1284 cc as,region
R264 T1291 T1290 advmod well,as
R268 T1296 T1300 nsubj protein,localizes
R269 T1297 T1298 compound mr,s
R271 T1299 T1298 punct -,s
R278 T1307 T1300 advcl overexpressed,localizes
R279 T1308 T1309 compound mr,s
R281 T1310 T1309 punct -,s
R282 T1311 T1307 auxpass is,overexpressed
R291 T1322 T1320 pobj assays,in
R292 T1323 T1322 compound luciferase,assays
R296 T1327 T1318 ccomp functions,found
R297 T1328 T1329 compound mr,s
R299 T1330 T1329 punct -,s
R300 T1331 T1327 nsubj protein,functions
R301 T1332 T1331 acl fused,protein
R302 T1333 T1332 prep to,fused
R303 T1334 T1335 nmod GAL4,domain
R304 T1335 T1333 pobj domain,to
R305 T1336 T1337 npadvmod DNA,binding
R306 T1337 T1335 amod binding,domain
R307 T1338 T1337 punct -,binding
R310 T1341 T1339 pobj repressor,as
R311 T1342 T1341 amod transcriptional,repressor
R315 T1348 T1346 ccomp is,revealed
R316 T1349 T1350 det the,activity
R318 T1351 T1350 compound repression,activity
R319 T1352 T1350 prep of,activity
R320 T1353 T1354 compound mr,s
R322 T1355 T1354 punct -,s
R327 T1360 T1358 pobj interaction,to
R328 T1361 T1360 amod homophilic,interaction
R331 T1364 T1362 pobj domain,through
R332 T1365 T1364 compound SAM,domain
R336 T1369 T1367 pobj region,to
R337 T1370 T1371 npadvmod C,terminal
R339 T1372 T1371 punct -,terminal
R343 T1380 T1378 dobj gene,identified
R344 T1381 T1380 amod novel,gene
R347 T1384 T1380 appos s,gene
R348 T1385 T1384 punct -,s
R351 T1388 T1380 relcl expressed,gene
R352 T1389 T1388 auxpass is,expressed
R353 T1390 T1388 advmod predominantly,expressed
R364 T1403 T1401 pobj pattern,on
R365 T1404 T1403 compound expression,pattern
R372 T1411 T1400 ccomp function,predict
R373 T1412 T1413 compound mr,s
R375 T1414 T1413 punct -,s
R376 T1415 T1411 aux may,function
R379 T1418 T1416 pobj repressor,as
R380 T1419 T1418 amod transcriptional,repressor
R389 T1428 T1426 pobj gland,of
R390 T1429 T1428 amod pineal,gland
R400 T3593 T3594 prep In,generated
R401 T3595 T3596 det the,development
R402 T3596 T3593 pobj development,In
R403 T3597 T3596 prep of,development
R404 T3598 T3599 det the,retina
R405 T3599 T3597 pobj retina,of
R406 T3600 T3599 amod mammalian,retina
R407 T3601 T3594 punct ", ",generated
R408 T3602 T3603 det a,range
R409 T3603 T3594 nsubjpass range,generated
R410 T3604 T3603 amod diverse,range
R411 T3605 T3603 prep of,range
R412 T3606 T3607 compound cell,types
R413 T3607 T3605 pobj types,of
R414 T3608 T3594 auxpass is,generated
R415 T3609 T3594 prep from,generated
R416 T3610 T3611 det a,pool
R417 T3611 T3609 pobj pool,from
R418 T3612 T3611 prep of,pool
R419 T3613 T3614 amod multipotent,cells
R420 T3614 T3612 pobj cells,of
R421 T3615 T3614 amod retinal,cells
R422 T3616 T3614 compound progenitor,cells
R423 T3617 T3594 punct .,generated
R424 T3619 T3620 prep Among,account
R425 T3621 T3622 det these,types
R426 T3622 T3619 pobj types,Among
R427 T3623 T3622 compound cell,types
R428 T3624 T3620 punct ", ",account
R429 T3629 T3626 pobj %,for
R430 T3625 T3620 nsubj photoreceptors,account
R431 T3630 T3629 prep of,%
R432 T3626 T3620 prep for,account
R433 T3631 T3632 det all,cells
R434 T3632 T3630 pobj cells,of
R435 T3633 T3629 prep in,%
R436 T3627 T3628 quantmod over,70
R437 T3628 T3629 nummod 70,%
R438 T3634 T3635 det the,retina
R439 T3635 T3633 pobj retina,in
R440 T3735 T3730 amod membranous,structure
R441 T3636 T3620 punct .,account
R442 T3638 T3639 prep In,are
R443 T3640 T3638 pobj vertebrates,In
R444 T3641 T3639 punct ", ",are
R445 T3642 T3639 expl there,are
R446 T3643 T3644 nummod two,classes
R447 T3644 T3639 attr classes,are
R448 T3645 T3644 prep of,classes
R449 T3646 T3645 pobj photoreceptors,of
R450 T3736 T3730 punct ", ",structure
R451 T3647 T3644 punct ", ",classes
R452 T3648 T3644 appos rods,classes
R453 T3649 T3648 cc and,rods
R454 T3650 T3648 conj cones,rods
R455 T3737 T3738 det the,segment
R456 T3651 T3639 punct .,are
R457 T3738 T3730 appos segment,structure
R458 T3653 T3654 nsubj Rods,are
R459 T3655 T3654 attr sensors,are
R460 T3739 T3738 amod outer,segment
R461 T3656 T3655 prep of,sensors
R462 T3657 T3658 amod dim,light
R463 T3658 T3656 pobj light,of
R464 T3740 T3722 punct .,located
R465 T3659 T3654 punct ", ",are
R466 T3660 T3661 mark while,function
R467 T3661 T3654 advcl function,are
R468 T3742 T3743 det The,segment
R469 T3662 T3661 nsubj cones,function
R470 T3663 T3661 prep in,function
R471 T3664 T3665 amod bright,light
R472 T3665 T3663 pobj light,in
R473 T3743 T3745 nsubj segment,appears
R474 T3666 T3661 cc and,function
R475 T3667 T3661 conj are,function
R476 T3668 T3667 acomp responsible,are
R477 T3744 T3743 amod outer,segment
R478 T3669 T3668 prep for,responsible
R479 T3670 T3671 compound color,vision
R480 T3671 T3669 pobj vision,for
R481 T3746 T3747 aux to,be
R482 T3672 T3654 punct .,are
R483 T3674 T3675 nsubjpass Phototransduction,initiated
R484 T3747 T3745 xcomp be,appears
R485 T3676 T3674 punct ", ",Phototransduction
R486 T3677 T3678 det a,series
R487 T3748 T3749 advmod relatively,fragile
R488 T3678 T3674 appos series,Phototransduction
R489 T3679 T3678 prep of,series
R490 T3680 T3681 compound signal,amplifications
R491 T3749 T3747 acomp fragile,be
R492 T3681 T3679 pobj amplifications,of
R493 T3750 T3747 punct ", ",be
R494 T3682 T3681 acl detecting,amplifications
R495 T3683 T3684 det a,photon
R496 T3684 T3682 dobj photon,detecting
R497 T3685 T3684 amod single,photon
R498 T3751 T3747 advcl degenerating,be
R499 T3686 T3684 prep of,photon
R500 T3687 T3686 pobj light,of
R501 T3688 T3675 punct ", ",initiated
R502 T3752 T3751 prep in,degenerating
R503 T3689 T3675 auxpass is,initiated
R504 T3690 T3675 agent by,initiated
R505 T3753 T3752 pobj response,in
R506 T3691 T3692 det the,capture
R507 T3692 T3690 pobj capture,by
R508 T3693 T3692 prep of,capture
R509 T3754 T3753 prep to,response
R510 T3694 T3693 pobj light,of
R511 T3695 T3675 prep with,initiated
R512 T3696 T3697 nummod 11,cis
R513 T3697 T3695 pobj cis,with
R514 T3755 T3756 amod many,perturbations
R515 T3698 T3697 punct -,cis
R516 T3699 T3697 punct -,cis
R517 T3700 T3697 amod retinal,cis
R518 T3756 T3754 pobj perturbations,to
R519 T3701 T3697 punct ", ",cis
R520 T3702 T3703 det a,chromophore
R521 T3757 T3756 amod environmental,perturbations
R522 T3703 T3697 appos chromophore,cis
R523 T3758 T3757 cc and,environmental
R524 T3704 T3703 acl bound,chromophore
R525 T3705 T3704 agent by,bound
R526 T3706 T3707 det the,proteins
R527 T3707 T3705 pobj proteins,by
R528 T3759 T3758 punct /,and
R529 T3708 T3707 compound opsin,proteins
R530 T3709 T3707 punct : ,proteins
R531 T3710 T3707 appos rhodopsin,proteins
R532 T3760 T3758 cc or,and
R533 T3711 T3710 prep in,rhodopsin
R534 T3712 T3711 pobj rods,in
R535 T3713 T3710 cc and,rhodopsin
R536 T3761 T3757 conj genetic,environmental
R537 T3714 T3715 compound cone,opsins
R538 T3715 T3710 conj opsins,rhodopsin
R539 T3716 T3715 prep in,opsins
R540 T3762 T3745 punct .,appears
R541 T3717 T3716 pobj cones,in
R542 T3718 T3675 punct .,initiated
R543 T3764 T3765 prep In,progresses
R544 T3720 T3721 det The,proteins
R545 T3721 T3722 nsubjpass proteins,located
R546 T3766 T3767 det the,retina
R547 T3723 T3724 dep that,carry
R548 T3724 T3721 relcl carry,proteins
R549 T3725 T3724 prt out,carry
R550 T3726 T3724 dobj phototransduction,carry
R551 T3767 T3764 pobj retina,In
R552 T3727 T3722 auxpass are,located
R553 T3728 T3722 prep in,located
R554 T3729 T3730 det an,structure
R555 T3768 T3767 compound rodent,retina
R556 T3730 T3728 pobj structure,in
R557 T3731 T3730 amod elaborate,structure
R558 T3732 T3731 cc and,elaborate
R559 T3769 T3765 punct ", ",progresses
R560 T3733 T3734 advmod highly,specialized
R561 T3734 T3731 conj specialized,elaborate
R562 T3770 T3771 det the,production
R563 T3771 T3765 nsubj production,progresses
R564 T3772 T3771 prep of,production
R565 T3773 T3774 amod specific,types
R566 T3774 T3772 pobj types,of
R567 T3841 T3842 det the,properties
R568 T3842 T3840 pobj properties,in
R569 T3775 T3774 compound cell,types
R570 T3843 T3842 amod intrinsic,properties
R571 T3844 T3842 prep of,properties
R572 T3845 T3846 compound progenitor,cells
R573 T3776 T3771 prep during,production
R574 T3846 T3844 pobj cells,of
R575 T3847 T3846 acl involved,cells
R576 T3848 T3847 prep in,involved
R577 T3777 T3776 pobj development,during
R578 T3849 T3850 det the,transcription
R579 T3850 T3848 pobj transcription,in
R580 T3851 T3850 cc or,transcription
R581 T3778 T3765 prep in,progresses
R582 T3852 T3853 compound chromatin,modification
R583 T3853 T3850 conj modification,transcription
R584 T3779 T3780 det a,order
R585 T3854 T3828 punct .,appears
R586 T3856 T3857 amod Recent,studies
R587 T3780 T3778 pobj order,in
R588 T3857 T3858 nsubj studies,identified
R589 T3859 T3860 amod several,factors
R590 T3781 T3780 amod general,order
R591 T3860 T3858 dobj factors,identified
R592 T3861 T3860 amod important,factors
R593 T3862 T3860 compound transcription,factors
R594 T3782 T3783 punct [,2
R595 T3863 T3860 prep of,factors
R596 T3864 T3865 compound photoreceptor,development
R597 T3865 T3863 pobj development,of
R598 T3783 T3765 parataxis 2,progresses
R599 T3866 T3867 punct [,3
R600 T3867 T3858 parataxis 3,identified
R601 T3868 T3869 punct -,7
R602 T3869 T3867 prep 7,3
R603 T3870 T3867 punct ],3
R604 T3871 T3858 punct .,identified
R605 T3784 T3783 nummod 1,2
R606 T3873 T3874 nummod Two,genes
R607 T3874 T3878 nsubj genes,play
R608 T3785 T3783 punct ",",2
R609 T3875 T3874 compound Otx,genes
R610 T3876 T3874 compound family,genes
R611 T3877 T3874 compound homeobox,genes
R612 T3786 T3783 punct ],2
R613 T3879 T3874 punct ", ",genes
R614 T3787 T3765 punct .,progresses
R615 T3880 T3874 appos Otx2,genes
R616 T3881 T3880 cc and,Otx2
R617 T3882 T3880 conj Crx,Otx2
R618 T3789 T3790 compound Rod,photoreceptor
R619 T3883 T3878 punct ", ",play
R620 T3884 T3885 amod essential,roles
R621 T3885 T3878 dobj roles,play
R622 T3790 T3791 compound photoreceptor,generation
R623 T3886 T3878 prep in,play
R624 T3887 T3888 amod early,determination
R625 T3888 T3886 pobj determination,in
R626 T3889 T3890 compound cell,fate
R627 T3791 T3792 nsubj generation,peaks
R628 T3890 T3888 compound fate,determination
R629 T3891 T3888 cc and,determination
R630 T3892 T3893 amod terminal,differentiation
R631 T3793 T3792 prep around,peaks
R632 T3893 T3888 conj differentiation,determination
R633 T3894 T3888 prep of,determination
R634 T3794 T3795 det the,time
R635 T3895 T3894 pobj photoreceptors,of
R636 T3896 T3897 punct [,9
R637 T3795 T3793 pobj time,around
R638 T3897 T3878 parataxis 9,play
R639 T3796 T3795 prep of,time
R640 T3898 T3897 nummod 3,9
R641 T3899 T3897 punct ",",9
R642 T3900 T3897 nummod 8,9
R643 T3797 T3796 pobj birth,of
R644 T3901 T3897 punct ",",9
R645 T3902 T3897 punct ],9
R646 T3798 T3792 punct .,peaks
R647 T3903 T3878 punct .,play
R648 T3905 T3906 prep In,converted
R649 T3800 T3801 compound Cone,photoreceptors
R650 T3907 T3908 det the,absence
R651 T3908 T3905 pobj absence,In
R652 T3909 T3908 prep of,absence
R653 T3910 T3911 compound Otx2,function
R654 T3911 T3909 pobj function,of
R655 T3801 T3802 nsubjpass photoreceptors,generated
R656 T3912 T3906 punct ", ",converted
R657 T3913 T3914 amod differentiating,cells
R658 T3914 T3906 nsubjpass cells,converted
R659 T3915 T3914 compound photoreceptor,cells
R660 T3803 T3801 punct ", ",photoreceptors
R661 T3916 T3906 auxpass are,converted
R662 T3917 T3906 prep to,converted
R663 T3918 T3919 npadvmod amacrine,like
R664 T3804 T3805 compound ganglion,cells
R665 T3919 T3921 amod like,neurons
R666 T3920 T3919 punct -,like
R667 T3921 T3917 pobj neurons,to
R668 T3805 T3801 conj cells,photoreceptors
R669 T3922 T3923 punct [,9
R670 T3923 T3906 parataxis 9,converted
R671 T3924 T3923 punct ],9
R672 T3806 T3805 punct ", ",cells
R673 T3925 T3906 punct .,converted
R674 T3927 T3928 nsubj Crx,controls
R675 T3807 T3808 amod horizontal,cells
R676 T3929 T3927 punct ", ",Crx
R677 T3930 T3931 det a,target
R678 T3808 T3805 conj cells,cells
R679 T3931 T3927 appos target,Crx
R680 T3932 T3931 amod downstream,target
R681 T3933 T3931 prep of,target
R682 T3809 T3808 cc and,cells
R683 T3934 T3933 pobj Otx2,of
R684 T3935 T3928 punct ", ",controls
R685 T3936 T3937 det the,transcription
R686 T3810 T3811 compound amacrine,cells
R687 T3937 T3928 dobj transcription,controls
R688 T3938 T3937 prep of,transcription
R689 T3939 T3940 amod various,genes
R690 T3811 T3808 conj cells,cells
R691 T3940 T3938 pobj genes,of
R692 T3941 T3940 nmod photoreceptor,genes
R693 T3812 T3802 auxpass are,generated
R694 T3942 T3943 npadvmod cell,specific
R695 T3943 T3940 amod specific,genes
R696 T3944 T3943 punct -,specific
R697 T3813 T3802 advmod earlier,generated
R698 T3945 T3928 cc and,controls
R699 T3946 T3928 conj is,controls
R700 T3814 T3802 punct ", ",generated
R701 T3815 T3816 mark while,generated
R702 T3816 T3802 advcl generated,generated
R703 T3947 T3946 acomp essential,is
R704 T3817 T3818 compound Müller,glia
R705 T3948 T3947 prep for,essential
R706 T3949 T3950 det the,formation
R707 T3950 T3948 pobj formation,for
R708 T3951 T3950 prep of,formation
R709 T3952 T3953 amod outer,segments
R710 T3953 T3951 pobj segments,of
R711 T3818 T3816 nsubjpass glia,generated
R712 T3954 T3953 punct ", ",segments
R713 T3955 T3956 amod synaptic,terminals
R714 T3956 T3953 conj terminals,segments
R715 T3819 T3818 cc and,glia
R716 T3957 T3956 punct ", ",terminals
R717 T3958 T3956 cc and,terminals
R718 T3959 T3960 compound phototransduction,pathways
R719 T3820 T3821 amod bipolar,cells
R720 T3960 T3956 conj pathways,terminals
R721 T3821 T3818 conj cells,glia
R722 T3961 T3962 punct [,10
R723 T3962 T3946 parataxis 10,is
R724 T3963 T3962 nummod 8,10
R725 T3822 T3816 auxpass are,generated
R726 T3964 T3962 punct ",",10
R727 T3965 T3962 punct ],10
R728 T3966 T3928 punct .,controls
R729 T3823 T3816 advmod later,generated
R730 T3968 T3969 compound Crx,transcripts
R731 T3969 T3970 nsubj transcripts,begin
R732 T3824 T3802 punct .,generated
R733 T3971 T3972 aux to,expressed
R734 T3826 T3827 det This,production
R735 T3972 T3970 xcomp expressed,begin
R736 T3973 T3972 auxpass be,expressed
R737 T3974 T3972 prep in,expressed
R738 T3975 T3976 amod developing,photoreceptors
R739 T3976 T3974 pobj photoreceptors,in
R740 T3827 T3828 nsubj production,appears
R741 T3977 T3972 prep at,expressed
R742 T3978 T3979 amod embryonic,day
R743 T3829 T3827 prep of,production
R744 T3979 T3977 pobj day,at
R745 T3980 T3979 nummod 12.5,day
R746 T3981 T3979 punct (,day
R747 T3830 T3831 amod different,types
R748 T3982 T3979 appos E12.5,day
R749 T3983 T3977 punct ),at
R750 T3984 T3972 prep in,expressed
R751 T3985 T3986 det the,mouse
R752 T3831 T3829 pobj types,of
R753 T3832 T3831 compound cell,types
R756 T3833 T3827 prep at,production
R757 T3988 T3989 det a,upregulation
R759 T3990 T3989 amod strong,upregulation
R760 T3991 T3970 conj is,begin
R761 T3992 T3989 prep of,upregulation
R762 T3993 T3994 compound Crx,transcription
R763 T3994 T3992 pobj transcription,of
R764 T3834 T3835 amod different,times
R765 T3995 T3991 acomp apparent,is
R766 T3996 T3991 prep across,is
R767 T3835 T3833 pobj times,at
R768 T3997 T3998 det the,photoreceptors
R769 T3998 T3996 pobj photoreceptors,across
R770 T3999 T3998 amod differentiating,photoreceptors
R771 T3836 T3837 aux to,derive
R772 T4000 T3991 prep at,is
R773 T4001 T4002 amod postnatal,day
R774 T4002 T4000 pobj day,at
R775 T3837 T3828 xcomp derive,appears
R776 T4003 T4002 nummod 6,day
R777 T4004 T4002 punct (,day
R778 T4005 T4002 appos P6,day
R779 T3838 T3837 prep from,derive
R780 T4006 T3991 punct ),is
R781 T4007 T3991 punct .,is
R782 T3839 T3838 pobj differences,from
R783 T4009 T4010 compound Photoreceptor,cells
R784 T4010 T4011 nsubj cells,exhibit
R785 T3840 T3839 prep in,differences
R786 T4012 T4010 prep in,cells
R787 T4013 T4014 det the,mice
R788 T4053 T4054 amod human,CRX
R789 T4014 T4012 pobj mice,in
R790 T4015 T4016 nmod Crx,knockout
R791 T4016 T4014 nmod knockout,mice
R792 T4017 T4016 punct (,knockout
R793 T4018 T4016 appos KO,knockout
R794 T4054 T4052 pobj CRX,of
R795 T4019 T4014 punct ),mice
R796 T4020 T4021 det a,reduction
R797 T4055 T4048 aux have,demonstrated
R798 T4021 T4011 dobj reduction,exhibit
R799 T4022 T4021 amod dramatic,reduction
R800 T4056 T4048 auxpass been,demonstrated
R801 T4023 T4021 prep of,reduction
R802 T4024 T4025 amod many,molecules
R803 T4025 T4023 pobj molecules,of
R804 T4026 T4025 compound photoreceptor,molecules
R805 T4057 T4058 aux to,associated
R806 T4027 T4025 prep including,molecules
R807 T4028 T4029 amod visual,pigments
R808 T4058 T4048 xcomp associated,demonstrated
R809 T4029 T4027 pobj pigments,including
R810 T4030 T4011 cc and,exhibit
R811 T4031 T4011 conj develop,exhibit
R812 T4032 T4033 preconj neither,segments
R813 T4033 T4031 dobj segments,develop
R814 T4034 T4033 nmod photoreceptor,segments
R815 T4035 T4033 amod outer,segments
R816 T4059 T4058 auxpass be,associated
R817 T4036 T4033 cc nor,segments
R818 T4037 T4038 det a,terminus
R819 T4038 T4033 conj terminus,segments
R820 T4060 T4058 prep with,associated
R821 T4039 T4038 amod synaptic,terminus
R822 T4040 T4041 punct [,10
R823 T4041 T4031 parataxis 10,develop
R824 T4061 T4062 nummod three,types
R825 T4042 T4041 nummod 8,10
R826 T4043 T4041 punct ",",10
R827 T4044 T4041 punct ],10
R828 T4062 T4060 pobj types,with
R829 T4045 T4011 punct .,exhibit
R830 T4063 T4062 prep of,types
R831 T4047 T4048 prep In,demonstrated
R832 T4049 T4047 pobj addition,In
R833 T4050 T4048 punct ", ",demonstrated
R834 T4064 T4065 compound photoreceptor,diseases
R835 T4051 T4048 nsubjpass mutations,demonstrated
R836 T4052 T4051 prep of,mutations
R837 T4065 T4063 pobj diseases,of
R838 T4066 T4062 punct : ,types
R839 T4067 T4068 amod autosomal,dominant
R840 T4068 T4069 amod dominant,dystrophy
R841 T4069 T4062 appos dystrophy,types
R842 T4159 T4160 compound protein,protein
R843 T4160 T4162 compound protein,interaction
R844 T4070 T4071 compound cone,rod
R845 T4161 T4160 punct -,protein
R846 T4162 T4158 compound interaction,modules
R847 T4071 T4069 compound rod,dystrophy
R848 T4163 T4164 punct [,15
R849 T4164 T4154 parataxis 15,reported
R850 T4072 T4071 punct -,rod
R851 T4165 T4166 punct -,17
R852 T4166 T4164 prep 17,15
R853 T4167 T4164 punct ],15
R854 T4073 T4069 nummod 2,dystrophy
R855 T4168 T4135 punct .,are
R856 T4074 T4069 punct ", ",dystrophy
R857 T4170 T4171 det This,domain
R858 T4171 T4172 nsubjpass domain,found
R859 T4075 T4076 amod autosomal,dominant
R860 T4173 T4172 auxpass is,found
R861 T4174 T4172 prep in,found
R862 T4175 T4176 det a,number
R863 T4176 T4174 pobj number,in
R864 T4177 T4176 amod large,number
R865 T4178 T4176 prep of,number
R866 T4076 T4077 amod dominant,type
R867 T4179 T4178 pobj proteins,of
R868 T4180 T4176 punct ", ",number
R869 T4181 T4176 prep including,number
R870 T4182 T4183 nmod Polycomb,group
R871 T4077 T4079 compound type,pigmentosa
R872 T4183 T4184 nmod group,proteins
R873 T4184 T4181 pobj proteins,including
R874 T4185 T4183 punct (,group
R875 T4078 T4077 punct -,type
R876 T4186 T4183 appos PcG,group
R877 T4187 T4184 punct ),proteins
R878 T4079 T4069 conj pigmentosa,dystrophy
R879 T4188 T4189 punct [,18
R880 T4189 T4184 parataxis 18,proteins
R881 T4190 T4189 punct ],18
R882 T4191 T4184 punct ", ",proteins
R883 T4080 T4079 compound retinitis,pigmentosa
R884 T4192 T4193 compound serine,kinases
R885 T4193 T4184 conj kinases,proteins
R886 T4081 T4079 punct ", ",pigmentosa
R887 T4194 T4193 compound threonine,kinases
R888 T4195 T4196 punct [,19
R889 T4196 T4193 parataxis 19,kinases
R890 T4082 T4079 cc and,pigmentosa
R891 T4197 T4196 punct ],19
R892 T4198 T4193 punct ", ",kinases
R893 T4199 T4200 compound Eph,family
R894 T4083 T4084 poss Leber,amaurosis
R895 T4200 T4201 compound family,kinases
R896 T4201 T4193 conj kinases,kinases
R897 T4202 T4201 compound receptor,kinases
R898 T4084 T4079 conj amaurosis,pigmentosa
R899 T4203 T4201 compound tyrosine,kinases
R900 T4204 T4205 punct [,20
R901 T4085 T4083 case 's,Leber
R902 T4205 T4201 parataxis 20,kinases
R903 T4206 T4205 punct ],20
R904 T4207 T4201 punct ", ",kinases
R905 T4086 T4084 amod congenital,amaurosis
R906 T4208 T4209 det the,suppressor
R907 T4087 T4084 punct (,amaurosis
R908 T4209 T4201 conj suppressor,kinases
R909 T4210 T4209 compound p73,suppressor
R910 T4211 T4209 compound tumor,suppressor
R911 T4212 T4213 punct [,21
R912 T4088 T4084 appos LCA,amaurosis
R913 T4213 T4209 parataxis 21,suppressor
R914 T4214 T4213 punct ],21
R915 T4215 T4209 punct ", ",suppressor
R916 T4216 T4217 det the,protein
R917 T4217 T4209 conj protein,suppressor
R918 T4218 T4219 npadvmod RNA,binding
R919 T4089 T4048 punct ),demonstrated
R920 T4219 T4217 amod binding,protein
R921 T4220 T4219 punct -,binding
R922 T4221 T4217 appos Smaug,protein
R923 T4090 T4091 punct [,11
R924 T4222 T4223 punct [,22
R925 T4223 T4217 parataxis 22,protein
R926 T4224 T4223 punct ],22
R927 T4091 T4048 parataxis 11,demonstrated
R928 T4225 T4217 punct ", ",protein
R929 T4226 T4227 compound diacylglycerol,kinases
R930 T4227 T4217 conj kinases,protein
R931 T4092 T4093 punct -,14
R932 T4228 T4229 punct [,24
R933 T4229 T4227 parataxis 24,kinases
R934 T4230 T4229 nummod 23,24
R935 T4093 T4091 prep 14,11
R936 T4231 T4229 punct ",",24
R937 T4232 T4229 punct ],24
R938 T4094 T4091 punct ],11
R939 T4233 T4227 punct ", ",kinases
R940 T4234 T4235 compound yeast,type
R941 T4235 T4237 compound type,proteins
R942 T4236 T4235 compound mating,type
R943 T4095 T4048 punct .,demonstrated
R944 T4237 T4227 conj proteins,kinases
R945 T4238 T4237 compound signaling,proteins
R946 T4097 T4098 mark While,control
R947 T4239 T4240 punct [,25
R948 T4240 T4237 parataxis 25,proteins
R949 T4098 T4102 advcl control,regulate
R950 T4241 T4240 nummod 19,25
R951 T4242 T4240 punct ",",25
R952 T4243 T4240 punct ],25
R953 T4099 T4098 nsubj Otx2,control
R954 T4244 T4237 cc and,proteins
R955 T4245 T4246 compound ETS,family
R956 T4246 T4247 compound family,factors
R957 T4100 T4099 cc and,Otx2
R958 T4247 T4237 conj factors,proteins
R959 T4101 T4099 conj Crx,Otx2
R960 T4248 T4247 compound transcription,factors
R961 T4249 T4250 punct [,27
R962 T4250 T4247 parataxis 27,factors
R963 T4251 T4250 nummod 26,27
R964 T4103 T4104 amod general,development
R965 T4252 T4250 punct ",",27
R966 T4253 T4250 punct ],27
R967 T4254 T4172 punct .,found
R968 T4256 T4257 det The,proteins
R969 T4257 T4259 nsubj proteins,are
R970 T4104 T4098 dobj development,control
R971 T4258 T4257 compound PcG,proteins
R972 T4260 T4261 amod transcriptional,repressors
R973 T4261 T4259 attr repressors,are
R974 T4105 T4104 compound photoreceptor,development
R975 T4262 T4263 dep that,maintain
R976 T4106 T4102 punct ", ",regulate
R977 T4107 T4108 nummod three,factors
R978 T4108 T4102 nsubj factors,regulate
R979 T4109 T4108 amod other,factors
R980 T4263 T4261 relcl maintain,repressors
R981 T4264 T4265 compound gene,silencing
R982 T4110 T4108 compound transcription,factors
R983 T4265 T4263 dobj silencing,maintain
R984 T4266 T4263 prep during,maintain
R985 T4111 T4108 punct ", ",factors
R986 T4267 T4266 pobj development,during
R987 T4268 T4269 punct [,28
R988 T4112 T4108 appos TRβ2,factors
R989 T4269 T4259 parataxis 28,are
R990 T4270 T4271 punct -,30
R991 T4271 T4269 prep 30,28
R992 T4113 T4112 punct ", ",TRβ2
R993 T4272 T4269 punct ],28
R994 T4273 T4259 punct .,are
R995 T4114 T4112 conj Nrl,TRβ2
R996 T4275 T4276 prep In,implicated
R997 T4277 T4275 pobj mammals,In
R998 T4115 T4114 punct ", ",Nrl
R999 T4278 T4276 punct ", ",implicated
R1000 T4279 T4280 compound PcG,proteins
R1001 T4116 T4114 cc and,Nrl
R1002 T4280 T4276 nsubjpass proteins,implicated
R1003 T4281 T4276 auxpass are,implicated
R1004 T4282 T4276 advmod also,implicated
R1005 T4117 T4114 conj Nr2e3,Nrl
R1006 T4283 T4276 prep in,implicated
R1007 T4284 T4285 compound Hox,gene
R1008 T4118 T4119 det the,specification
R1009 T4285 T4286 compound gene,regulation
R1010 T4286 T4283 pobj regulation,in
R1011 T4287 T4276 punct .,implicated
R1012 T4119 T4102 dobj specification,regulate
R1013 T4289 T4290 poss Their,activity
R1014 T4290 T4292 nsubj activity,lies
R1015 T4120 T4119 prep of,specification
R1016 T4291 T4290 amod biological,activity
R1017 T4121 T4122 compound photoreceptor,types
R1018 T4293 T4292 prep in,lies
R1019 T4294 T4295 amod stable,silencing
R1020 T4295 T4293 pobj silencing,in
R1021 T4296 T4295 prep of,silencing
R1022 T4297 T4298 amod specific,sets
R1023 T4298 T4296 pobj sets,of
R1024 T4122 T4120 pobj types,of
R1025 T4299 T4298 prep of,sets
R1026 T4300 T4299 pobj genes,of
R1027 T4301 T4295 prep through,silencing
R1028 T4123 T4122 compound cell,types
R1029 T4302 T4303 compound chromatin,modifications
R1030 T4303 T4301 pobj modifications,through
R1031 T4304 T4292 punct .,lies
R1032 T4124 T4125 punct [,7
R1033 T4306 T4307 det A,member
R1034 T4125 T4102 parataxis 7,regulate
R1035 T4307 T4308 nsubj member,contains
R1036 T4309 T4307 prep of,member
R1037 T4126 T4125 nummod 4,7
R1038 T4310 T4311 nmod polycomb,complex
R1039 T4311 T4309 pobj complex,of
R1040 T4312 T4311 amod repressive,complex
R1041 T4127 T4125 punct ",",7
R1042 T4313 T4311 nummod 1,complex
R1043 T4314 T4311 punct (,complex
R1044 T4128 T4125 nummod 5,7
R1045 T4315 T4311 appos PRC1,complex
R1046 T4316 T4307 punct ),member
R1047 T4317 T4307 punct ", ",member
R1048 T4129 T4125 punct ",",7
R1049 T4318 T4307 appos ph,member
R1050 T4319 T4308 punct ", ",contains
R1051 T4320 T4321 det a,domain
R1052 T4130 T4125 punct ],7
R1053 T4321 T4308 dobj domain,contains
R1054 T4322 T4321 compound SAM,domain
R1055 T4131 T4102 punct .,regulate
R1056 T4323 T4308 prep at,contains
R1057 T4324 T4325 det the,terminal
R1058 T4325 T4323 pobj terminal,at
R1059 T4133 T4134 compound SAM,domains
R1060 T4326 T4325 compound C,terminal
R1061 T4327 T4325 punct -,terminal
R1062 T4134 T4135 nsubj domains,are
R1063 T4328 T4308 punct ", ",contains
R1064 T4136 T4134 punct (,domains
R1065 T4329 T4308 cc and,contains
R1066 T4330 T4331 compound PRC1,complex
R1067 T4331 T4332 nsubjpass complex,known
R1068 T4332 T4308 conj known,contains
R1069 T4137 T4138 advmod also,known
R1070 T4333 T4332 auxpass is,known
R1071 T4334 T4335 aux to,form
R1072 T4138 T4134 acl known,domains
R1073 T4335 T4332 xcomp form,known
R1074 T4336 T4337 amod helical,polymers
R1075 T4337 T4335 dobj polymers,form
R1076 T4338 T4337 punct ", ",polymers
R1077 T4339 T4337 nmod head,polymers
R1078 T4139 T4138 prep as,known
R1079 T4340 T4339 punct -,head
R1080 T4341 T4339 prep to,head
R1081 T4140 T4141 amod Pointed,domains
R1082 T4342 T4341 punct -,to
R1083 T4343 T4341 pobj tail,to
R1084 T4344 T4335 prep through,form
R1085 T4345 T4346 poss its,domain
R1086 T4141 T4139 pobj domains,as
R1087 T4346 T4344 pobj domain,through
R1088 T4347 T4346 compound SAM,domain
R1089 T4142 T4140 punct ", ",Pointed
R1090 T4348 T4349 punct [,31
R1091 T4349 T4335 parataxis 31,form
R1092 T4350 T4349 punct ],31
R1093 T4143 T4140 conj HLH,Pointed
R1094 T4351 T4332 punct .,known
R1095 T4353 T4354 det These,structures
R1096 T4144 T4143 punct ", ",HLH
R1097 T4354 T4356 nsubj structures,mediate
R1098 T4355 T4354 amod polymeric,structures
R1099 T4145 T4143 cc or,HLH
R1100 T4357 T4358 det the,formation
R1101 T4358 T4356 dobj formation,mediate
R1102 T4359 T4358 prep of,formation
R1103 T4146 T4143 conj SPM,HLH
R1104 T4360 T4361 det a,structure
R1105 T4361 T4359 pobj structure,of
R1106 T4147 T4134 punct ),domains
R1107 T4362 T4363 amod higher,order
R1108 T4363 T4361 compound order,structure
R1109 T4364 T4361 compound chromatin,structure
R1110 T4148 T4149 punct ~,70
R1111 T4365 T4356 cc and,mediate
R1112 T4366 T4367 advmod possibly,stabilize
R1113 T4149 T4150 nummod 70,residues
R1114 T4367 T4356 conj stabilize,mediate
R1115 T4150 T4151 npadvmod residues,long
R1116 T4151 T4135 acomp long,are
R1117 T4368 T4369 det the,state
R1119 T4152 T4135 cc and,are
R1120 T4370 T4369 amod repressed,state
R1121 T4371 T4369 prep of,state
R1122 T4153 T4154 aux have,reported
R1123 T4372 T4373 compound Hox,genes
R1124 T4373 T4371 pobj genes,of
R1125 T4374 T4356 punct .,mediate
R1126 T4376 T4377 prep In,identified
R1127 T4154 T4135 conj reported,are
R1128 T4378 T4379 det this,study
R1129 T4379 T4376 pobj study,In
R1130 T4380 T4377 punct ", ",identified
R1131 T4155 T4154 auxpass been,reported
R1132 T4381 T4377 nsubj we,identified
R1133 T4382 T4383 det a,gene
R1134 T4383 T4377 dobj gene,identified
R1135 T4156 T4154 prep as,reported
R1136 T4384 T4383 amod novel,gene
R1137 T4385 T4383 punct ", ",gene
R1138 T4386 T4387 compound mr,s
R1139 T4157 T4158 amod common,modules
R1140 T4387 T4383 appos s,gene
R1141 T4388 T4387 punct -,s
R1142 T4389 T4383 punct ", ",gene
R1143 T4158 T4156 pobj modules,as
R1144 T4390 T4391 dep which,encodes
R1145 T4391 T4383 relcl encodes,gene
R1146 T4392 T4393 det a,protein
R1147 T4474 T4472 pobj region,through
R1148 T4393 T4391 dobj protein,encodes
R1149 T4394 T4395 compound SAM,domain
R1150 T4395 T4396 npadvmod domain,containing
R1151 T4396 T4393 amod containing,protein
R1152 T4397 T4396 punct -,containing
R1153 T4398 T4377 punct .,identified
R1154 T4475 T4476 compound SAM,domain
R1155 T4400 T4401 det The,domain
R1156 T4401 T4403 nsubjpass domain,related
R1157 T4476 T4477 npadvmod domain,containing
R1158 T4402 T4401 compound SAM,domain
R1159 T4404 T4401 prep of,domain
R1160 T4477 T4474 amod containing,region
R1161 T4405 T4406 compound mr,s
R1162 T4406 T4404 pobj s,of
R1163 T4478 T4477 punct -,containing
R1164 T4407 T4406 punct -,s
R1165 T4408 T4403 auxpass is,related
R1166 T4409 T4410 advmod most,closely
R1167 T4479 T4458 punct .,localized
R1168 T4410 T4403 advmod closely,related
R1169 T4411 T4403 prep to,related
R1170 T4412 T4411 pobj that,to
R1171 T4481 T4482 nsubj We,found
R1172 T4413 T4412 prep of,that
R1173 T4414 T4415 compound ph,ortholog
R1174 T4483 T4482 advmod also,found
R1175 T4415 T4413 pobj ortholog,of
R1176 T4416 T4415 compound mouse,ortholog
R1177 T4417 T4415 punct ", ",ortholog
R1178 T4418 T4419 compound MPH1,Rae28
R1179 T4484 T4485 mark that,functions
R1180 T4419 T4415 appos Rae28,ortholog
R1181 T4420 T4419 punct /,Rae28
R1182 T4421 T4403 punct .,related
R1183 T4485 T4482 ccomp functions,found
R1184 T4423 T4424 compound mr,s
R1185 T4486 T4487 compound mr,s
R1186 T4424 T4426 nsubjpass s,expressed
R1187 T4425 T4424 punct -,s
R1188 T4487 T4489 compound s,protein
R1189 T4427 T4426 auxpass is,expressed
R1190 T4428 T4426 advmod predominantly,expressed
R1191 T4488 T4487 punct -,s
R1192 T4429 T4426 prep in,expressed
R1193 T4430 T4431 amod retinal,photoreceptors
R1194 T4431 T4429 pobj photoreceptors,in
R1195 T4489 T4485 nsubj protein,functions
R1196 T4432 T4433 advmod when,undergo
R1197 T4433 T4431 advcl undergo,photoreceptors
R1198 T4434 T4433 nsubj they,undergo
R1199 T4490 T4489 acl fused,protein
R1200 T4435 T4436 amod terminal,differentiation
R1201 T4436 T4433 dobj differentiation,undergo
R1202 T4437 T4431 punct ", ",photoreceptors
R1203 T4491 T4490 prep to,fused
R1204 T4438 T4431 cc and,photoreceptors
R1205 T4439 T4440 amod adult,gland
R1206 T4440 T4431 conj gland,photoreceptors
R1207 T4492 T4493 nmod GAL4,domain
R1208 T4441 T4440 amod pineal,gland
R1209 T4442 T4426 punct .,expressed
R1210 T4493 T4491 pobj domain,to
R1211 T4444 T4445 det The,expression
R1212 T4445 T4446 nsubjpass expression,regulated
R1213 T4494 T4495 npadvmod DNA,binding
R1214 T4447 T4445 prep of,expression
R1215 T4448 T4449 compound mr,s
R1216 T4495 T4493 amod binding,domain
R1217 T4449 T4447 pobj s,of
R1218 T4450 T4449 punct -,s
R1219 T4451 T4446 auxpass is,regulated
R1220 T4496 T4495 punct -,binding
R1221 T4452 T4446 advmod directly,regulated
R1222 T4453 T4446 agent by,regulated
R1223 T4454 T4453 pobj Crx,by
R1224 T4497 T4485 prep as,functions
R1225 T4455 T4446 punct .,regulated
R1226 T4498 T4499 det a,repressor
R1227 T4457 T4458 advmod Moreover,localized
R1228 T4499 T4497 pobj repressor,as
R1229 T4459 T4458 punct ", ",localized
R1230 T4460 T4461 compound mr,s
R1231 T4461 T4458 nsubjpass s,localized
R1232 T4462 T4461 punct -,s
R1233 T4463 T4458 auxpass is,localized
R1234 T4500 T4499 amod transcriptional,repressor
R1235 T4464 T4458 prep in,localized
R1236 T4465 T4466 det the,nucleus
R1239 T4466 T4464 pobj nucleus,in
R1240 T4467 T4458 cc and,localized
R1241 T4504 T4505 nsubj findings,suggest
R1242 T4468 T4469 aux can,associate
R1243 T4469 T4458 conj associate,localized
R1244 T4506 T4507 mark that,functions
R1245 T4470 T4469 dep self,associate
R1246 T4471 T4469 punct -,associate
R1247 T4472 T4469 prep through,associate
R1248 T4473 T4474 poss its,region
R1249 T4507 T4505 ccomp functions,suggest
R1250 T4508 T4509 compound mr,s
R1251 T4509 T4507 nsubj s,functions
R1252 T4510 T4509 punct -,s
R1253 T4511 T4507 prep as,functions
R1254 T4512 T4513 det a,member
R1255 T4513 T4511 pobj member,as
R1256 T4514 T4513 prep of,member
R1257 T4515 T4516 det a,complex
R1258 T4516 T4514 pobj complex,of
R1259 T4517 T4516 amod transcriptional,complex
R1260 T4518 T4516 compound repressor,complex
R1261 T4519 T4507 prep in,functions
R1262 T4520 T4521 amod retinal,development
R1263 T4521 T4519 pobj development,in
R1264 T4522 T4521 compound photoreceptor,development
R1265 T4523 T4505 punct .,suggest
R1266 T5547 T5546 prep of,Cloning
R1267 T5548 T5549 compound mouse,s
R1268 T5549 T5547 pobj s,of
R1269 T5550 T5549 compound mr,s
R1270 T5551 T5549 punct -,s
R1271 T5553 T5554 prep In,screened
R1272 T5555 T5553 pobj order,In
R1273 T5556 T5557 aux to,identify
R1274 T5557 T5555 acl identify,order
R1275 T5558 T5559 amod novel,genes
R1276 T5559 T5557 dobj genes,identify
R1277 T5560 T5559 compound mouse,genes
R1278 T5561 T5562 advmod preferentially,expressed
R1279 T5562 T5559 acl expressed,genes
R1280 T5563 T5562 prep in,expressed
R1281 T5564 T5565 det the,retina
R1282 T5565 T5563 pobj retina,in
R1283 T5566 T5565 amod developing,retina
R1284 T5567 T5554 punct ", ",screened
R1285 T5568 T5554 nsubj we,screened
R1286 T5569 T5570 det the,database
R1288 T5571 T5572 nmod National,Institute
R1289 T5572 T5570 nmod Institute,database
R1290 T5573 T5572 prep for,Institute
R1291 T5574 T5575 compound Biotechnology,Information
R1292 T5575 T5573 pobj Information,for
R1293 T5576 T5572 punct (,Institute
R1294 T5577 T5572 appos NCBI,Institute
R1295 T5578 T5570 punct ),database
R1296 T5579 T5570 punct ", ",database
R1297 T5580 T5570 appos UniGene,database
R1298 T5581 T5554 punct ", ",screened
R1299 T5582 T5554 advcl using,screened
R1300 T5583 T5584 compound Digital,Display
R1301 T5584 T5582 dobj Display,using
R1302 T5585 T5584 compound Differential,Display
R1303 T5586 T5584 punct (,Display
R1304 T5587 T5584 appos DDD,Display
R1305 T5588 T5554 punct ),screened
R1306 T5589 T5554 cc and,screened
R1307 T5590 T5554 conj found,screened
R1308 T5591 T5592 compound EST,fragments
R1309 T5592 T5590 dobj fragments,found
R1310 T5593 T5594 dep which,are
R1311 T5594 T5592 relcl are,fragments
R1312 T5595 T5594 advmod frequently,are
R1313 T5596 T5594 acomp present,are
R1314 T5597 T5594 prep in,are
R1315 T5598 T5599 nmod mouse,libraries
R1316 T5599 T5597 pobj libraries,in
R1317 T5600 T5599 amod retinal,libraries
R1318 T5601 T5599 compound cDNA,libraries
R1319 T5602 T5554 punct .,screened
R1320 T5604 T5605 nsubj We,found
R1321 T5606 T5607 mark that,encodes
R1322 T5607 T5605 ccomp encodes,found
R1323 T5608 T5609 nummod one,clone
R1324 T5609 T5607 nsubj clone,encodes
R1325 T5610 T5609 prep in,clone
R1326 T5611 T5612 det these,cDNAs
R1327 T5612 T5610 pobj cDNAs,in
R1328 T5613 T5614 det a,protein
R1329 T5614 T5607 dobj protein,encodes
R1330 T5615 T5614 acl containing,protein
R1331 T5616 T5617 det a,domain
R1332 T5617 T5615 dobj domain,containing
R1333 T5618 T5617 compound SAM,domain
R1334 T5619 T5617 acl related,domain
R1335 T5620 T5619 prep to,related
R1336 T5621 T5620 pobj that,to
R1337 T5622 T5621 prep of,that
R1338 T5623 T5624 amod polyhomeotic,protein
R1339 T5624 T5622 pobj protein,of
R1340 T5625 T5605 punct .,found
R1341 T5627 T5628 det A,fragment
R1342 T5628 T5630 nsubjpass fragment,used
R1343 T5629 T5628 compound PCR,fragment
R1344 T5631 T5628 acl corresponding,fragment
R1345 T5632 T5631 prep to,corresponding
R1346 T5633 T5634 det this,clone
R1347 T5634 T5632 pobj clone,to
R1348 T5635 T5634 compound mouse,clone
R1349 T5636 T5630 auxpass was,used
R1350 T5637 T5638 aux to,screen
R1351 T5638 T5630 advcl screen,used
R1352 T5639 T5640 det a,library
R1353 T5640 T5638 dobj library,screen
R1354 T5641 T5640 nmod mouse,library
R1355 T5642 T5643 nmod P0,P3
R1356 T5643 T5640 nmod P3,library
R1357 T5644 T5643 punct -,P3
R1358 T5645 T5640 amod retinal,library
R1359 T5646 T5640 compound cDNA,library
R1360 T5647 T5648 aux to,obtain
R1361 T5648 T5638 advcl obtain,screen
R1362 T5649 T5650 det a,clone
R1363 T5650 T5648 dobj clone,obtain
R1364 T5651 T5652 amod full,length
R1365 T5652 T5650 compound length,clone
R1366 T5653 T5652 punct -,length
R1367 T5654 T5650 compound cDNA,clone
R1368 T5655 T5630 punct .,used
R1369 T5657 T5658 compound Sequence,analysis
R1370 T5658 T5659 nsubj analysis,showed
R1371 T5660 T5661 mark that,was
R1372 T5661 T5659 ccomp was,showed
R1373 T5662 T5663 det this,cDNA
R1374 T5663 T5661 nsubj cDNA,was
R1375 T5664 T5665 det a,gene
R1376 T5665 T5661 attr gene,was
R1377 T5666 T5665 amod novel,gene
R1378 T5667 T5665 acl encoding,gene
R1379 T5668 T5669 det a,protein
R1380 T5669 T5667 dobj protein,encoding
R1381 T5670 T5671 compound SAM,domain
R1382 T5671 T5672 npadvmod domain,containing
R1383 T5672 T5669 amod containing,protein
R1384 T5673 T5672 punct -,containing
R1385 T5674 T5659 punct .,showed
R1386 T5676 T5677 nsubj We,referred
R1387 T5678 T5677 prep to,referred
R1388 T5679 T5680 det this,protein
R1389 T5680 T5678 pobj protein,to
R1390 T5681 T5677 prep as,referred
R1391 T5682 T5683 compound mr,s
R1392 T5683 T5681 pobj s,as
R1393 T5684 T5683 punct -,s
R1394 T5685 T5683 punct (,s
R1395 T5686 T5687 amod major,protein
R1396 T5687 T5683 appos protein,s
R1397 T5688 T5687 amod retinal,protein
R1398 T5689 T5690 compound SAM,domain
R1399 T5690 T5687 compound domain,protein
R1400 T5691 T5677 punct ),referred
R1401 T5692 T5677 punct .,referred
R1402 T5694 T5695 mark As,shown
R1403 T5695 T5696 advcl shown,is
R1404 T5697 T5695 prep in,shown
R1405 T5698 T5699 compound Fig.,1A
R1406 T5699 T5697 pobj 1A,in
R1407 T5700 T5696 punct ", ",is
R1408 T5701 T5702 det a,codon
R1409 T5702 T5696 nsubj codon,is
R1410 T5703 T5702 compound translation,codon
R1411 T5704 T5702 compound initiation,codon
R1412 T5705 T5696 acomp present,is
R1413 T5706 T5696 prep in,is
R1414 T5707 T5708 det the,frame
R1415 T5708 T5706 pobj frame,in
R1416 T5709 T5708 amod same,frame
R1417 T5710 T5708 amod open,frame
R1418 T5711 T5708 compound reading,frame
R1419 T5712 T5708 prep as,frame
R1420 T5713 T5714 det the,domain
R1421 T5714 T5712 pobj domain,as
R1422 T5715 T5714 compound SAM,domain
R1423 T5716 T5696 punct .,is
R1424 T5718 T5719 det This,site
R1425 T5719 T5721 nsubj site,shows
R1426 T5720 T5719 compound initiation,site
R1427 T5722 T5721 dobj similarity,shows
R1428 T5723 T5722 prep to,similarity
R1429 T5724 T5725 det the,sequence
R1430 T5725 T5723 pobj sequence,to
R1431 T5726 T5725 compound consensus,sequence
R1432 T5727 T5725 acl proposed,sequence
R1433 T5728 T5727 agent by,proposed
R1434 T5729 T5728 pobj Kozak,by
R1435 T5730 T5731 punct [,32
R1436 T5731 T5729 parataxis 32,Kozak
R1437 T5732 T5731 punct ],32
R1438 T5733 T5725 prep including,sequence
R1439 T5734 T5735 det the,presence
R1440 T5735 T5733 pobj presence,including
R1441 T5736 T5735 prep of,presence
R1442 T5737 T5738 det the,purine
R1443 T5738 T5736 pobj purine,of
R1444 T5739 T5740 advmod highly,conserved
R1445 T5740 T5738 amod conserved,purine
R1446 T5741 T5735 prep at,presence
R1447 T5742 T5741 pobj position,at
R1448 T5743 T5742 punct -,position
R1449 T5744 T5742 nummod 3,position
R1450 T5745 T5721 punct .,shows
R1451 T5747 T5748 det The,codon
R1452 T5748 T5750 nsubjpass codon,indicated
R1453 T5749 T5748 compound stop,codon
R1454 T5751 T5748 prep of,codon
R1455 T5752 T5753 det the,protein
R1456 T5753 T5751 pobj protein,of
R1457 T5754 T5753 amod predicted,protein
R1458 T5755 T5756 compound mr,s
R1459 T5756 T5753 compound s,protein
R1460 T5757 T5756 punct -,s
R1461 T5758 T5750 auxpass is,indicated
R1462 T5759 T5750 advmod also,indicated
R1463 T5760 T5750 prep in,indicated
R1464 T5761 T5762 compound Fig.,1A
R1465 T5762 T5760 pobj 1A,in
R1466 T5763 T5750 punct .,indicated
R1467 T5765 T5766 det The,sequence
R1468 T5766 T5769 nsubj sequence,exhibits
R1469 T5767 T5768 compound amino,acid
R1470 T5768 T5766 compound acid,sequence
R1471 T5770 T5766 prep of,sequence
R1472 T5771 T5772 det the,domain
R1473 T5772 T5770 pobj domain,of
R1474 T5773 T5772 compound SAM,domain
R1475 T5774 T5772 prep of,domain
R1476 T5775 T5776 compound mr,s
R1477 T5776 T5778 compound s,protein
R1478 T5777 T5776 punct -,s
R1479 T5778 T5774 pobj protein,of
R1480 T5779 T5780 punct (,1A
R1481 T5780 T5766 parataxis 1A,sequence
R1482 T5781 T5780 compound Fig.,1A
R1483 T5782 T5780 punct ", ",1A
R1484 T5783 T5784 amod boxed,sequence
R1485 T5784 T5780 appos sequence,1A
R1486 T5785 T5780 punct ),1A
R1487 T5786 T5769 dobj homology,exhibits
R1488 T5787 T5786 prep with,homology
R1489 T5788 T5789 compound SAM,domains
R1490 T5789 T5787 pobj domains,with
R1491 T5790 T5789 prep of,domains
R1492 T5791 T5790 pobj EphB2,of
R1493 T5792 T5791 punct ", ",EphB2
R1494 T5793 T5791 conj EphA4,EphB2
R1495 T5794 T5793 punct ", ",EphA4
R1496 T5795 T5793 conj MPH1,EphA4
R1497 T5796 T5795 punct ", ",MPH1
R1498 T5797 T5795 conj TEL,MPH1
R1499 T5798 T5797 cc and,TEL
R1500 T5799 T5797 conj Smaug,TEL
R1501 T5800 T5801 punct (,1B
R1502 T5801 T5769 parataxis 1B,exhibits
R1503 T5802 T5801 compound Fig.,1B
R1504 T5803 T5801 punct ),1B
R1505 T5804 T5769 punct .,exhibits
R1506 T5806 T5807 prep By,related
R1507 T5808 T5809 amod phylogenetic,analysis
R1508 T5809 T5806 pobj analysis,By
R1509 T5810 T5807 punct ", ",related
R1510 T5811 T5812 det the,domain
R1511 T5812 T5807 nsubjpass domain,related
R1512 T5813 T5812 compound SAM,domain
R1513 T5814 T5812 prep of,domain
R1514 T5815 T5816 compound mr,s
R1515 T5816 T5814 pobj s,of
R1516 T5817 T5816 punct -,s
R1517 T5818 T5807 auxpass is,related
R1518 T5819 T5820 advmod most,closely
R1519 T5820 T5807 advmod closely,related
R1520 T5821 T5807 prep to,related
R1521 T5822 T5821 pobj that,to
R1522 T5823 T5822 prep of,that
R1523 T5824 T5825 compound Mph1,Rae28
R1524 T5825 T5823 pobj Rae28,of
R1525 T5826 T5825 punct /,Rae28
R1526 T5827 T5825 punct ", ",Rae28
R1527 T5828 T5829 det a,homolog
R1528 T5829 T5825 appos homolog,Rae28
R1529 T5830 T5829 compound mouse,homolog
R1530 T5831 T5829 prep of,homolog
R1531 T5832 T5831 pobj ph,of
R1532 T5833 T5834 punct (,1C
R1533 T5834 T5807 parataxis 1C,related
R1534 T5835 T5834 compound Fig.,1C
R1535 T5836 T5834 punct ),1C
R1536 T5837 T5807 punct .,related
R1537 T5839 T5840 compound Mouse,protein
R1538 T5840 T5844 nsubjpass protein,conserved
R1539 T5841 T5842 compound mr,s
R1540 T5842 T5840 compound s,protein
R1541 T5843 T5842 punct -,s
R1542 T5845 T5844 auxpass is,conserved
R1543 T5846 T5844 prep in,conserved
R1544 T5847 T5846 pobj rat,in
R1545 T5848 T5847 punct ", ",rat
R1546 T5849 T5847 amod human,rat
R1547 T5850 T5847 punct ", ",rat
R1548 T5851 T5847 conj chick,rat
R1549 T5852 T5851 cc and,chick
R1550 T5853 T5851 conj zebrafish,chick
R1551 T5854 T5847 punct ", ",rat
R1552 T5855 T5856 dep which,display
R1553 T5856 T5847 relcl display,rat
R1554 T5857 T5858 nummod 91,%
R1555 T5858 T5859 nmod %,identity
R1556 T5859 T5856 dobj identity,display
R1557 T5860 T5858 punct ", ",%
R1558 T5861 T5862 nummod 70,%
R1559 T5862 T5858 conj %,%
R1560 T5863 T5862 punct ", ",%
R1561 T5864 T5865 nummod 36,%
R1562 T5865 T5862 conj %,%
R1563 T5866 T5865 cc and,%
R1564 T5867 T5868 nummod 26,%
R1565 T5868 T5865 conj %,%
R1566 T5869 T5859 prep with,identity
R1567 T5870 T5871 compound mouse,s
R1568 T5871 T5869 pobj s,with
R1569 T5872 T5871 compound mr,s
R1570 T5873 T5871 punct -,s
R1571 T5874 T5856 punct ", ",display
R1572 T5875 T5856 advmod respectively,display
R1573 T5876 T5877 punct (,1D
R1574 T5877 T5844 parataxis 1D,conserved
R1575 T5878 T5877 compound Fig.,1D
R1576 T5879 T5877 punct ),1D
R1577 T5880 T5844 punct .,conserved
R1578 T5882 T5883 det The,domains
R1579 T5883 T5885 nsubjpass domains,conserved
R1580 T5884 T5883 compound SAM,domains
R1581 T5886 T5883 prep of,domains
R1582 T5887 T5888 nmod rat,protein
R1583 T5888 T5886 pobj protein,of
R1584 T5889 T5887 punct ", ",rat
R1585 T5890 T5887 amod human,rat
R1586 T5891 T5887 punct ", ",rat
R1587 T5892 T5887 conj chick,rat
R1588 T5893 T5892 cc and,chick
R1589 T5894 T5892 conj zebrafish,chick
R1590 T5895 T5896 compound mr,s
R1591 T5896 T5888 compound s,protein
R1592 T5897 T5896 punct -,s
R1593 T5898 T5885 auxpass are,conserved
R1594 T5899 T5885 advmod highly,conserved
R1595 T5900 T5885 cc and,conserved
R1596 T5901 T5885 conj display,conserved
R1597 T5902 T5903 nummod 96,%
R1598 T5903 T5904 nmod %,identity
R1599 T5904 T5901 dobj identity,display
R1600 T5905 T5903 punct ", ",%
R1601 T5906 T5907 nummod 90,%
R1602 T5907 T5903 conj %,%
R1603 T5908 T5907 punct ", ",%
R1604 T5909 T5910 nummod 76,%
R1605 T5910 T5907 conj %,%
R1606 T5911 T5910 cc and,%
R1607 T5912 T5913 nummod 72,%
R1608 T5913 T5910 conj %,%
R1609 T5914 T5904 prep with,identity
R1610 T5915 T5916 det the,domain
R1611 T5916 T5914 pobj domain,with
R1612 T5917 T5916 compound SAM,domain
R1613 T5918 T5916 prep of,domain
R1614 T5919 T5920 compound mouse,protein
R1615 T5920 T5918 pobj protein,of
R1616 T5921 T5922 compound mr,s
R1617 T5922 T5920 compound s,protein
R1618 T5923 T5922 punct -,s
R1619 T5924 T5885 punct .,conserved
R1620 T5926 T5927 det The,localizations
R1621 T5927 T5929 nsubjpass localizations,determined
R1622 T5928 T5927 amod chromosomal,localizations
R1623 T5930 T5927 prep of,localizations
R1624 T5931 T5932 nmod mouse,genes
R1625 T5932 T5930 pobj genes,of
R1626 T5933 T5931 cc and,mouse
R1627 T5934 T5931 conj human,mouse
R1628 T5935 T5936 compound mr,s
R1629 T5936 T5932 compound s,genes
R1630 T5937 T5936 punct -,s
R1631 T5938 T5929 auxpass were,determined
R1632 T5939 T5929 prep by,determined
R1633 T5940 T5939 pcomp searching,by
R1634 T5941 T5942 det the,databases
R1635 T5942 T5940 dobj databases,searching
R1636 T5943 T5942 nmod mouse,databases
R1637 T5944 T5943 cc and,mouse
R1638 T5945 T5943 conj human,mouse
R1639 T5946 T5942 compound genome,databases
R1640 T5947 T5948 punct (,NCBI
R1641 T5948 T5942 parataxis NCBI,databases
R1642 T5949 T5948 punct ),NCBI
R1643 T5950 T5940 punct ", ",searching
R1644 T5951 T5940 advmod respectively,searching
R1645 T5952 T5929 punct .,determined
R1646 T5954 T5955 compound Mouse,s
R1647 T5955 T5958 nsubjpass s,mapped
R1648 T5956 T5955 compound mr,s
R1649 T5957 T5955 punct -,s
R1650 T5959 T5958 auxpass is,mapped
R1651 T5960 T5958 prep to,mapped
R1652 T5961 T5962 compound chromosome,4E2
R1653 T5962 T5960 pobj 4E2,to
R1654 T5963 T5958 punct ", ",mapped
R1655 T5964 T5958 cc and,mapped
R1656 T5965 T5966 amod human,S
R1657 T5966 T5969 nsubjpass S,mapped
R1658 T5967 T5966 compound MR,S
R1659 T5968 T5966 punct -,S
R1660 T5969 T5958 conj mapped,mapped
R1661 T5970 T5969 auxpass is,mapped
R1662 T5971 T5969 prep to,mapped
R1663 T5972 T5973 compound chromosome,1p36.33
R1664 T5973 T5971 pobj 1p36.33,to
R1665 T5974 T5969 punct .,mapped
R1666 T5976 T5977 nsubj LCA,is
R1667 T5978 T5979 det the,cause
R1668 T5979 T5977 attr cause,is
R1669 T5980 T5981 advmod most,common
R1670 T5981 T5979 amod common,cause
R1671 T5982 T5979 prep of,cause
R1672 T5983 T5984 amod inherited,blindness
R1673 T5984 T5982 pobj blindness,of
R1674 T5985 T5984 compound childhood,blindness
R1675 T5986 T5977 punct .,is
R1676 T5988 T5989 amod Human,S
R1677 T5989 T5992 nsubj S,maps
R1678 T5990 T5989 compound MR,S
R1679 T5991 T5989 punct -,S
R1680 T5993 T5992 prep in,maps
R1681 T5994 T5995 det the,region
R1682 T5995 T5993 pobj region,in
R1683 T5996 T5995 compound vicinity,region
R1684 T5997 T5995 prep of,region
R1685 T5998 T5999 det the,LCA9
R1686 T5999 T5997 pobj LCA9,of
R1687 T6000 T5999 punct ", ",LCA9
R1688 T6001 T6002 advmod recently,identified
R1689 T6002 T5999 acl identified,LCA9
R1690 T6003 T6002 prep as,identified
R1691 T6004 T6005 det a,locus
R1692 T6005 T6003 pobj locus,as
R1693 T6006 T6005 amod new,locus
R1694 T6007 T6005 prep for,locus
R1695 T6008 T6007 pobj LCA,for
R1696 T6009 T6010 punct [,33
R1697 T6010 T5992 parataxis 33,maps
R1698 T6011 T6010 punct ],33
R1699 T6012 T5992 punct .,maps
R1702 T7366 T7365 prep of,Expression
R1703 T7367 T7368 compound mr,s
R1704 T7368 T7366 pobj s,of
R1705 T7369 T7368 punct -,s
R1706 T7370 T7365 prep in,Expression
R1707 T7371 T7372 det the,retina
R1708 T7372 T7370 pobj retina,in
R1709 T7373 T7372 amod developing,retina
R1710 T7374 T7372 cc and,retina
R1711 T7375 T7376 det the,gland
R1712 T7376 T7372 conj gland,retina
R1713 T7377 T7376 amod pineal,gland
R1714 T7379 T7380 aux To,investigate
R1715 T7380 T7381 advcl investigate,performed
R1716 T7382 T7383 compound mr,s
R1717 T7383 T7385 compound s,expression
R1718 T7384 T7383 punct -,s
R1719 T7385 T7380 dobj expression,investigate
R1720 T7386 T7381 punct ", ",performed
R1721 T7387 T7381 nsubj we,performed
R1722 T7388 T7381 advmod first,performed
R1723 T7389 T7390 amod whole,mount
R1724 T7390 T7391 nmod mount,hybridization
R1725 T7391 T7381 dobj hybridization,performed
R1726 T7392 T7393 advmod in,situ
R1727 T7393 T7391 amod situ,hybridization
R1728 T7394 T7381 prep in,performed
R1729 T7395 T7396 compound mouse,embryos
R1730 T7396 T7394 pobj embryos,in
R1731 T7397 T7381 punct .,performed
R1732 T7399 T7400 det No,signal
R1733 T7400 T7402 nsubjpass signal,detected
R1734 T7401 T7400 compound hybridization,signal
R1735 T7403 T7402 auxpass was,detected
R1736 T7404 T7402 prep at,detected
R1737 T7405 T7404 pobj E9.5,at
R1738 T7406 T7405 punct ", ",E9.5
R1739 T7407 T7405 conj E10.5,E9.5
R1740 T7408 T7407 cc and,E10.5
R1741 T7409 T7407 conj E11.5,E10.5
R1742 T7410 T7402 prep with,detected
R1743 T7411 T7412 det an,probe
R1744 T7412 T7410 pobj probe,with
R1745 T7413 T7414 compound mr,s
R1746 T7414 T7412 compound s,probe
R1747 T7415 T7414 punct -,s
R1748 T7416 T7417 punct (,shown
R1749 T7417 T7402 parataxis shown,detected
R1750 T7418 T7417 nsubj data,shown
R1751 T7419 T7417 neg not,shown
R1752 T7420 T7417 punct ),shown
R1753 T7421 T7402 punct .,detected
R1754 T7423 T7424 nsubj We,investigated
R1755 T7425 T7424 advmod then,investigated
R1756 T7426 T7427 det the,expression
R1757 T7427 T7424 dobj expression,investigated
R1758 T7428 T7427 prep of,expression
R1759 T7429 T7430 det the,gene
R1760 T7430 T7428 pobj gene,of
R1761 T7431 T7432 compound mr,s
R1762 T7432 T7430 compound s,gene
R1763 T7433 T7432 punct -,s
R1764 T7434 T7424 prep in,investigated
R1765 T7435 T7436 det the,retina
R1766 T7436 T7434 pobj retina,in
R1767 T7437 T7436 amod developing,retina
R1768 T7438 T7424 prep by,investigated
R1769 T7439 T7440 nmod section,hybridization
R1770 T7440 T7438 pobj hybridization,by
R1771 T7441 T7442 advmod in,situ
R1772 T7442 T7440 amod situ,hybridization
R1773 T7443 T7444 punct (,1A
R1774 T7444 T7424 parataxis 1A,investigated
R1775 T7445 T7444 compound Fig.,1A
R1776 T7446 T7447 punct –,G
R1777 T7447 T7444 prep G,1A
R1778 T7448 T7444 punct ),1A
R1779 T7449 T7424 punct .,investigated
R1780 T7451 T7452 det No,signal
R1781 T7452 T7454 nsubjpass signal,detected
R1782 T7453 T7452 amod significant,signal
R1783 T7455 T7454 auxpass was,detected
R1784 T7456 T7454 prep in,detected
R1785 T7457 T7458 det the,retina
R1786 T7458 T7456 pobj retina,in
R1787 T7459 T7458 amod developing,retina
R1788 T7460 T7454 prep at,detected
R1789 T7461 T7460 pobj E13,at
R1790 T7462 T7463 punct (,2A
R1791 T7463 T7454 parataxis 2A,detected
R1792 T7464 T7463 compound Fig.,2A
R1793 T7465 T7463 punct ),2A
R1794 T7466 T7454 punct .,detected
R1795 T7468 T7469 det A,signal
R1796 T7469 T7471 nsubjpass signal,detected
R1797 T7470 T7469 amod weak,signal
R1798 T7472 T7471 auxpass was,detected
R1799 T7473 T7471 advmod initially,detected
R1800 T7474 T7471 prep in,detected
R1801 T7475 T7476 det the,aspect
R1802 T7476 T7474 pobj aspect,in
R1803 T7477 T7476 amod outer,aspect
R1804 T7478 T7476 prep of,aspect
R1805 T7479 T7480 det the,layer
R1806 T7480 T7478 pobj layer,of
R1807 T7481 T7480 amod neuroblastic,layer
R1808 T7482 T7480 punct (,layer
R1809 T7483 T7480 appos NBL,layer
R1810 T7484 T7480 punct ),layer
R1811 T7485 T7480 punct ", ",layer
R1812 T7486 T7487 det a,layer
R1813 T7487 T7480 appos layer,layer
R1814 T7488 T7487 amod presumptive,layer
R1815 T7489 T7487 compound photoreceptor,layer
R1816 T7490 T7471 prep at,detected
R1817 T7491 T7490 pobj E18,at
R1818 T7492 T7493 punct (,2B
R1819 T7493 T7471 parataxis 2B,detected
R1820 T7494 T7493 compound Fig.,2B
R1821 T7495 T7493 punct ", ",2B
R1822 T7496 T7493 appos arrow,2B
R1823 T7497 T7493 punct ),2B
R1824 T7498 T7471 punct .,detected
R1825 T7500 T7501 prep At,detected
R1826 T7502 T7500 pobj P3,At
R1827 T7503 T7501 punct ", ",detected
R1828 T7504 T7505 det an,transcript
R1829 T7505 T7501 nsubjpass transcript,detected
R1830 T7506 T7507 compound mr,s
R1831 T7507 T7505 compound s,transcript
R1832 T7508 T7507 punct -,s
R1833 T7509 T7501 auxpass was,detected
R1834 T7510 T7501 advmod clearly,detected
R1835 T7511 T7501 prep in,detected
R1836 T7512 T7513 det the,layer
R1837 T7513 T7511 pobj layer,in
R1838 T7514 T7513 amod developing,layer
R1839 T7515 T7513 compound photoreceptor,layer
R1840 T7516 T7517 punct (,2E
R1841 T7517 T7501 parataxis 2E,detected
R1842 T7518 T7517 compound Fig.,2E
R1843 T7519 T7517 punct ),2E
R1844 T7520 T7501 punct .,detected
R1845 T7522 T7523 prep At,showed
R1846 T7524 T7522 pobj P6,At
R1847 T7525 T7523 punct ", ",showed
R1848 T7526 T7527 compound mr,s
R1849 T7527 T7523 nsubj s,showed
R1850 T7528 T7527 punct -,s
R1851 T7529 T7530 compound peak,expression
R1852 T7530 T7523 dobj expression,showed
R1853 T7531 T7523 prep in,showed
R1854 T7532 T7533 det the,layer
R1855 T7533 T7531 pobj layer,in
R1856 T7534 T7533 compound photoreceptor,layer
R1857 T7535 T7536 punct (,2F
R1858 T7536 T7523 parataxis 2F,showed
R1859 T7537 T7536 compound Fig.,2F
R1860 T7538 T7536 punct ),2F
R1861 T7539 T7523 punct .,showed
R1862 T7541 T7542 det This,pattern
R1863 T7542 T7543 nsubj pattern,correlates
R1864 T7544 T7543 prep with,correlates
R1865 T7545 T7546 det the,increase
R1866 T7546 T7544 pobj increase,with
R1867 T7547 T7546 amod rapid,increase
R1868 T7548 T7546 prep in,increase
R1869 T7549 T7548 pobj cells,in
R1870 T7550 T7549 acl expressing,cells
R1871 T7551 T7550 dobj rhodopsin,expressing
R1872 T7552 T7551 cc and,rhodopsin
R1873 T7553 T7554 amod other,genes
R1874 T7554 T7551 conj genes,rhodopsin
R1875 T7555 T7554 compound phototransduction,genes
R1876 T7556 T7546 prep between,increase
R1877 T7557 T7556 pobj P6,between
R1878 T7558 T7559 punct -,P8
R1879 T7559 T7557 prep P8,P6
R1880 T7560 T7561 punct [,34
R1881 T7561 T7543 parataxis 34,correlates
R1882 T7562 T7563 punct -,37
R1883 T7563 T7561 prep 37,34
R1884 T7564 T7561 punct ],34
R1885 T7565 T7543 punct .,correlates
R1886 T7567 T7568 prep At,reduced
R1887 T7569 T7567 pobj P9,At
R1888 T7570 T7568 punct ", ",reduced
R1889 T7571 T7572 det the,intensity
R1890 T7572 T7568 nsubjpass intensity,reduced
R1891 T7573 T7572 prep of,intensity
R1892 T7574 T7575 det the,signal
R1893 T7575 T7573 pobj signal,of
R1894 T7576 T7577 compound mr,s
R1895 T7577 T7575 compound s,signal
R1896 T7578 T7577 punct -,s
R1897 T7579 T7568 auxpass was,reduced
R1898 T7580 T7568 advmod significantly,reduced
R1899 T7581 T7568 cc but,reduced
R1900 T7582 T7583 auxpass was,localized
R1901 T7583 T7568 conj localized,reduced
R1902 T7584 T7583 prep to,localized
R1970 T7683 T7684 prep As,used
R1971 T7685 T7686 det a,control
R1972 T7686 T7683 pobj control,As
R1973 T7687 T7684 punct ", ",used
R1974 T7688 T7689 compound P7,RNA
R1975 T7689 T7684 nsubjpass RNA,used
R1976 T7690 T7689 compound retinal,RNA
R1977 T7691 T7684 auxpass was,used
R1978 T7692 T7684 punct .,used
R1979 T7694 T7695 nummod Four,bands
R1980 T7695 T7696 nsubjpass bands,detected
R1981 T7697 T7695 acl corresponding,bands
R1982 T7698 T7697 prep to,corresponding
R1983 T7699 T7700 nummod 7.2,kb
R1984 T7700 T7698 pobj kb,to
R1985 T7701 T7700 punct -,kb
R1986 T7702 T7700 punct ", ",kb
R1987 T7703 T7704 nummod 4.0,kb
R1988 T7704 T7700 conj kb,kb
R1989 T7705 T7704 punct -,kb
R1990 T7706 T7704 punct ", ",kb
R1991 T7707 T7708 nummod 2.5,kb
R1992 T7708 T7704 conj kb,kb
R1993 T7709 T7708 punct -,kb
R1994 T7710 T7708 cc and,kb
R1995 T7711 T7712 nummod 2.2,kb
R1996 T7712 T7708 conj kb,kb
R1997 T7713 T7712 punct -,kb
R1998 T7714 T7696 auxpass were,detected
R1999 T7715 T7696 prep in,detected
R2000 T7716 T7717 compound P7,retina
R2001 T7717 T7715 pobj retina,in
R2002 T7718 T7696 punct .,detected
R2003 T7720 T7721 det The,band
R2004 T7721 T7725 nsubj band,corresponds
R2005 T7722 T7723 nummod 2.2,kb
R2006 T7723 T7721 compound kb,band
R2007 T7724 T7723 punct -,kb
R2008 T7726 T7725 prep to,corresponds
R2009 T7727 T7728 det the,cDNA
R2010 T7728 T7726 pobj cDNA,to
R2011 T7729 T7728 acl characterized,cDNA
R2012 T7730 T7729 prep in,characterized
R2013 T7731 T7732 det this,study
R2014 T7732 T7730 pobj study,in
R2015 T7733 T7725 punct .,corresponds
R2016 T7735 T7736 det The,bands
R2017 T7736 T7738 nsubjpass bands,characterized
R2018 T7737 T7736 amod larger,bands
R2019 T7739 T7736 punct ", ",bands
R2020 T7740 T7741 advmod possibly,transcripts
R2021 T7741 T7736 appos transcripts,bands
R2022 T7742 T7743 amod alternative,spliced
R2023 T7743 T7741 amod spliced,transcripts
R2024 T7744 T7738 punct ", ",characterized
R2025 T7745 T7738 aux have,characterized
R2026 T7746 T7738 neg not,characterized
R2027 T7747 T7738 advmod yet,characterized
R2028 T7748 T7738 auxpass been,characterized
R2029 T7749 T7738 punct .,characterized
R2030 T7751 T7752 det The,probe
R2031 T7752 T7756 nsubj probe,detect
R2032 T7753 T7754 compound mr,s
R2033 T7754 T7752 compound s,probe
R2034 T7755 T7754 punct -,s
R2035 T7757 T7756 aux did,detect
R2036 T7758 T7756 neg not,detect
R2037 T7759 T7760 det a,band
R2038 T7760 T7756 dobj band,detect
R2039 T7761 T7756 prep in,detect
R2040 T7762 T7763 det the,tissues
R2041 T7763 T7761 pobj tissues,in
R2042 T7764 T7763 amod adult,tissues
R2043 T7765 T7763 acl examined,tissues
R2044 T7766 T7756 punct ", ",detect
R2045 T7767 T7756 advcl indicating,detect
R2046 T7768 T7769 mark that,express
R2047 T7769 T7767 ccomp express,indicating
R2048 T7770 T7771 det these,tissues
R2049 T7771 T7769 nsubj tissues,express
R2050 T7772 T7769 aux do,express
R2051 T7773 T7769 neg not,express
R2052 T7774 T7775 compound mr,s
R2053 T7775 T7769 dobj s,express
R2054 T7776 T7775 punct -,s
R2055 T7777 T7769 prep at,express
R2056 T7778 T7779 det a,level
R2057 T7779 T7777 pobj level,at
R2058 T7780 T7779 amod comparable,level
R2059 T7781 T7780 prep to,comparable
R2060 T7782 T7781 pobj that,to
R2061 T7783 T7782 prep of,that
R2062 T7784 T7785 det the,retina
R2063 T7785 T7783 pobj retina,of
R2064 T7786 T7785 amod developing,retina
R2065 T7787 T7756 punct .,detect
R2066 T7789 T7790 amod Previous,reports
R2067 T7790 T7791 nsubj reports,revealed
R2068 T7792 T7793 mark that,expressed
R2069 T7793 T7791 ccomp expressed,revealed
R2070 T7794 T7795 amod many,genes
R2071 T7795 T7793 nsubjpass genes,expressed
R2072 T7796 T7797 npadvmod photoreceptor,specific
R2073 T7797 T7795 amod specific,genes
R2074 T7798 T7797 punct -,specific
R2075 T7799 T7793 auxpass are,expressed
R2076 T7800 T7793 advmod also,expressed
R2077 T7801 T7793 prep in,expressed
R2078 T7802 T7803 det the,gland
R2079 T7803 T7801 pobj gland,in
R2080 T7804 T7803 amod pineal,gland
R2081 T7805 T7806 punct [,38
R2082 T7806 T7791 parataxis 38,revealed
R2083 T7807 T7806 punct ],38
R2084 T7808 T7791 punct .,revealed
R2085 T7810 T7811 nsubj We,examined
R2086 T7812 T7813 det the,expression
R2087 T7813 T7811 dobj expression,examined
R2088 T7814 T7813 prep of,expression
R2089 T7815 T7816 compound mr,s
R2090 T7816 T7818 compound s,transcripts
R2091 T7817 T7816 punct -,s
R2092 T7818 T7814 pobj transcripts,of
R2093 T7819 T7813 prep in,expression
R2094 T7820 T7821 det the,embryo
R2095 T7821 T7819 pobj embryo,in
R2096 T7822 T7821 amod whole,embryo
R2097 T7823 T7821 punct ", ",embryo
R2098 T7824 T7825 amod whole,body
R2099 T7825 T7821 conj body,embryo
R2100 T7826 T7825 punct ", ",body
R2101 T7827 T7825 conj retina,body
R2102 T7828 T7827 punct ", ",retina
R2103 T7829 T7830 amod pineal,gland
R2104 T7830 T7827 conj gland,retina
R2105 T7831 T7830 punct ", ",gland
R2106 T7832 T7830 conj brain,gland
R2107 T7833 T7832 punct ", ",brain
R2108 T7834 T7832 conj liver,brain
R2109 T7835 T7834 cc and,liver
R2110 T7836 T7837 amod other,organs
R2111 T7837 T7834 conj organs,liver
R2112 T7838 T7811 prep at,examined
R2113 T7839 T7840 amod various,stages
R2114 T7840 T7838 pobj stages,at
R2115 T7841 T7811 prep by,examined
R2116 T7842 T7843 compound RT,PCR
R2117 T7843 T7841 pobj PCR,by
R2118 T7844 T7843 punct -,PCR
R2119 T7845 T7846 punct (,2I
R2120 T7846 T7811 parataxis 2I,examined
R2121 T7847 T7846 compound Fig.,2I
R2122 T7848 T7846 cc and,2I
R2123 T7849 T7850 nsubj data,shown
R2124 T7850 T7846 conj shown,2I
R2125 T7851 T7850 neg not,shown
R2126 T7852 T7846 punct ),2I
R2127 T7853 T7811 punct .,examined
R2128 T7855 T7856 nsubj We,amplified
R2129 T7857 T7858 compound PCR,fragments
R2130 T7858 T7856 dobj fragments,amplified
R2131 T7859 T7858 prep of,fragments
R2132 T7860 T7861 nummod 292,bp
R2133 T7861 T7859 pobj bp,of
R2134 T7862 T7861 cc and,bp
R2135 T7863 T7864 nummod 452,bp
R2136 T7864 T7861 conj bp,bp
R2137 T7865 T7856 prep with,amplified
R2138 T7866 T7867 compound primer,pairs
R2139 T7867 T7865 pobj pairs,with
R2140 T7868 T7867 prep for,pairs
R2141 T7869 T7868 pobj genes,for
R2142 T7870 T7869 acl encoding,genes
R2143 T7871 T7872 compound mouse,s
R2144 T7872 T7870 dobj s,encoding
R2145 T7873 T7872 compound mr,s
R2146 T7874 T7872 punct -,s
R2147 T7875 T7872 cc and,s
R2148 T7876 T7872 conj G3PDH,s
R2149 T7877 T7870 punct ", ",encoding
R2150 T7878 T7870 advmod respectively,encoding
R2151 T7879 T7856 punct .,amplified
R2152 T7881 T7882 prep In,detected
R2153 T7883 T7884 nmod E13,embryo
R2154 T7884 T7881 pobj embryo,In
R2155 T7885 T7884 amod whole,embryo
R2156 T7886 T7884 cc and,embryo
R2157 T7887 T7888 nmod P0,body
R2158 T7888 T7884 conj body,embryo
R2159 T7889 T7888 amod whole,body
R2160 T7890 T7888 punct (,body
R2161 T7891 T7888 prep except,body
R2162 T7892 T7891 prep for,except
R2163 T7893 T7894 det the,eye
R2164 T7894 T7892 pobj eye,for
R2165 T7895 T7882 punct ),detected
R2166 T7896 T7882 punct ", ",detected
R2167 T7897 T7898 det no,signal
R2168 T7898 T7882 nsubjpass signal,detected
R2169 T7899 T7900 compound mr,s
R2170 T7900 T7898 compound s,signal
R2171 T7901 T7900 punct -,s
R2172 T7902 T7882 auxpass was,detected
R2173 T7903 T7882 punct .,detected
R2174 T7905 T7906 mark As,expected
R2175 T7906 T7907 advcl expected,observed
R2176 T7908 T7907 punct ", ",observed
R2177 T7909 T7907 nsubj we,observed
R2178 T7910 T7911 mark that,expressed
R2179 T7911 T7907 ccomp expressed,observed
R2180 T7912 T7913 compound mr,s
R2181 T7913 T7911 nsubjpass s,expressed
R2182 T7914 T7913 punct -,s
R2183 T7915 T7911 auxpass is,expressed
R2184 T7916 T7911 prep in,expressed
R2185 T7917 T7918 det the,gland
R2186 T7918 T7916 pobj gland,in
R2187 T7919 T7918 nmod P7,gland
R2188 T7920 T7919 cc and,P7
R2189 T7921 T7919 conj adult,P7
R2190 T7922 T7918 amod pineal,gland
R2191 T7923 T7907 punct .,observed
R2192 T7925 T7926 prep In,detected
R2193 T7927 T7928 det the,brain
R2194 T7928 T7925 pobj brain,In
R2195 T7929 T7928 nmod P7,brain
R2196 T7930 T7929 cc and,P7
R2197 T7931 T7929 conj adult,P7
R2198 T7932 T7928 punct ", ",brain
R2199 T7933 T7928 conj liver,brain
R2200 T7934 T7933 cc and,liver
R2201 T7935 T7936 amod several,organs
R2202 T7936 T7933 conj organs,liver
R2203 T7937 T7936 amod other,organs
R2204 T7938 T7926 punct ", ",detected
R2205 T7939 T7940 det the,band
R2206 T7940 T7926 nsubjpass band,detected
R2207 T7941 T7942 compound RT,PCR
R2208 T7942 T7944 npadvmod PCR,amplified
R2209 T7943 T7942 punct -,PCR
R2210 T7944 T7940 amod amplified,band
R2211 T7945 T7940 prep of,band
R2212 T7946 T7947 compound mr,s
R2213 T7947 T7945 pobj s,of
R2214 T7948 T7947 punct -,s
R2215 T7949 T7926 auxpass was,detected
R2216 T7950 T7926 neg not,detected
R2217 T7951 T7952 punct (,2I
R2218 T7952 T7926 parataxis 2I,detected
R2219 T7953 T7952 compound Fig.,2I
R2220 T7954 T7952 cc and,2I
R2221 T7955 T7956 nsubj data,shown
R2222 T7956 T7952 conj shown,2I
R2223 T7957 T7956 neg not,shown
R2224 T7958 T7952 punct ),2I
R2225 T7959 T7926 punct .,detected
R2226 T7961 T7962 poss Our,data
R2227 T7962 T7963 nsubj data,showed
R2228 T7964 T7965 mark that,expressed
R2229 T7965 T7963 ccomp expressed,showed
R2230 T7966 T7967 compound mr,s
R2231 T7967 T7965 nsubjpass s,expressed
R2232 T7968 T7967 punct -,s
R2233 T7969 T7965 auxpass is,expressed
R2234 T7970 T7965 advmod predominantly,expressed
R2235 T7971 T7965 prep in,expressed
R2236 T7972 T7973 amod developing,photoreceptors
R2237 T7973 T7971 pobj photoreceptors,in
R2238 T7974 T7973 cc and,photoreceptors
R2239 T7975 T7976 det the,gland
R2240 T7976 T7973 conj gland,photoreceptors
R2241 T7977 T7976 amod pineal,gland
R2242 T7978 T7963 punct .,showed
R2243 T9716 T9715 prep of,Regulation
R2244 T9717 T9718 compound mr,s
R2245 T9718 T9720 compound s,transcription
R2246 T9719 T9718 punct -,s
R2247 T9720 T9716 pobj transcription,of
R2248 T9721 T9715 prep by,Regulation
R2249 T9722 T9723 compound Crx,protein
R2250 T9723 T9721 pobj protein,by
R2251 T9724 T9723 compound homeodomain,protein
R2252 T9726 T9727 compound Transcription,factors
R2253 T9727 T9728 nsubjpass factors,known
R2254 T9729 T9727 appos Otx2,factors
R2255 T9730 T9729 cc and,Otx2
R2256 T9731 T9729 conj Crx,Otx2
R2257 T9732 T9728 auxpass are,known
R2258 T9733 T9734 aux to,be
R2259 T9734 T9728 xcomp be,known
R2260 T9735 T9736 amod key,regulators
R2261 T9736 T9734 attr regulators,be
R2262 T9737 T9736 prep of,regulators
R2263 T9738 T9739 amod retinal,development
R2264 T9739 T9737 pobj development,of
R2265 T9740 T9739 compound photoreceptor,development
R2266 T9741 T9742 punct [,9
R2267 T9742 T9728 parataxis 9,known
R2268 T9743 T9742 nummod 3,9
R2269 T9744 T9742 punct ",",9
R2270 T9745 T9742 nummod 8,9
R2271 T9746 T9742 punct ",",9
R2272 T9747 T9742 punct ],9
R2273 T9748 T9728 punct .,known
R2274 T9750 T9751 mark Although,expressed
R2275 T9751 T9757 advcl expressed,expressed
R2276 T9752 T9753 preconj both,Otx2
R2277 T9753 T9751 nsubjpass Otx2,expressed
R2278 T9754 T9753 cc and,Otx2
R2279 T9755 T9753 conj Crx,Otx2
R2280 T9756 T9751 auxpass are,expressed
R2281 T9758 T9751 prep in,expressed
R2282 T9759 T9760 amod developing,cells
R2283 T9760 T9758 pobj cells,in
R2284 T9761 T9760 compound photoreceptor,cells
R2285 T9762 T9751 prep in,expressed
R2286 T9763 T9764 det the,retina
R2287 T9764 T9762 pobj retina,in
R2288 T9765 T9764 amod embryonic,retina
R2289 T9766 T9757 punct ", ",expressed
R2290 T9767 T9768 advmod only,Crx
R2291 T9768 T9757 nsubjpass Crx,expressed
R2292 T9769 T9757 auxpass is,expressed
R2293 T9770 T9757 advmod highly,expressed
R2294 T9771 T9757 prep in,expressed
R2295 T9772 T9773 det the,photoreceptors
R2296 T9773 T9771 pobj photoreceptors,in
R2297 T9774 T9773 amod postnatal,photoreceptors
R2298 T9775 T9757 punct ", ",expressed
R2299 T9776 T9757 advcl suggesting,expressed
R2300 T9777 T9778 mark that,regulated
R2301 T9778 T9776 ccomp regulated,suggesting
R2302 T9779 T9780 compound mr,s
R2303 T9780 T9778 nsubjpass s,regulated
R2304 T9781 T9780 punct -,s
R2305 T9782 T9778 aux may,regulated
R2306 T9783 T9778 auxpass be,regulated
R2307 T9784 T9778 agent by,regulated
R2308 T9785 T9784 pobj Crx,by
R2309 T9786 T9757 punct .,expressed
R2310 T9788 T9789 aux To,test
R2311 T9789 T9790 advcl test,performed
R2312 T9791 T9792 mark whether,regulates
R2313 T9792 T9789 ccomp regulates,test
R2314 T9793 T9792 nsubj Crx,regulates
R2315 T9794 T9795 compound mr,s
R2316 T9795 T9797 compound s,transcription
R2317 T9796 T9795 punct -,s
R2318 T9797 T9792 dobj transcription,regulates
R2319 T9798 T9790 punct ", ",performed
R2320 T9799 T9790 nsubj we,performed
R2321 T9800 T9801 advmod in,situ
R2322 T9801 T9802 amod situ,hybridization
R2323 T9802 T9790 dobj hybridization,performed
R2324 T9803 T9802 prep of,hybridization
R2325 T9804 T9805 compound mr,s
R2326 T9805 T9807 compound s,mRNA
R2327 T9806 T9805 punct -,s
R2328 T9807 T9803 pobj mRNA,of
R2329 T9808 T9790 prep on,performed
R2330 T9809 T9810 amod wild,type
R2331 T9810 T9812 nmod type,retinas
R2332 T9811 T9810 punct -,type
R2333 T9812 T9808 pobj retinas,on
R2334 T9813 T9810 cc and,type
R2335 T9814 T9815 compound Crx,KO
R2336 T9815 T9810 conj KO,type
R2337 T9816 T9812 compound P5,retinas
R2338 T9817 T9818 punct (,B
R2339 T9818 T9790 parataxis B,performed
R2340 T9819 T9818 nmod Fig.,B
R2341 T9820 T9818 nmod 3A,B
R2342 T9821 T9818 punct ", ",B
R2343 T9822 T9818 punct ),B
R2344 T9823 T9790 punct .,performed
R2345 T9825 T9826 prep In,reduced
R2346 T9827 T9828 det the,retina
R2347 T9828 T9825 pobj retina,In
R2348 T9829 T9830 compound Crx,KO
R2349 T9830 T9828 compound KO,retina
R2350 T9831 T9826 punct ", ",reduced
R2351 T9832 T9833 det the,transcript
R2352 T9833 T9826 nsubjpass transcript,reduced
R2353 T9834 T9835 compound mr,s
R2354 T9835 T9833 compound s,transcript
R2355 T9836 T9835 punct -,s
R2356 T9837 T9826 auxpass was,reduced
R2357 T9838 T9826 advmod dramatically,reduced
R2358 T9839 T9840 punct (,3B
R2359 T9840 T9826 parataxis 3B,reduced
R2360 T9841 T9840 compound Fig.,3B
R2361 T9842 T9840 punct ),3B
R2362 T9843 T9826 punct .,reduced
R2363 T9845 T9846 nsubj This,indicates
R2364 T9847 T9848 mark that,is
R2365 T9848 T9846 ccomp is,indicates
R2366 T9849 T9848 nsubj Crx,is
R2367 T9850 T9848 acomp essential,is
R2368 T9851 T9850 prep for,essential
R2369 T9852 T9851 pobj transactivation,for
R2370 T9853 T9852 prep of,transactivation
R2371 T9854 T9855 compound mr,s
R2372 T9855 T9853 pobj s,of
R2373 T9856 T9855 punct -,s
R2374 T9857 T9846 punct .,indicates
R2375 T9859 T9860 advmod Moreover,used
R2376 T9861 T9860 punct ", ",used
R2377 T9862 T9863 aux to,test
R2378 T9863 T9860 advcl test,used
R2379 T9864 T9865 mark whether,regulated
R2380 T9865 T9863 ccomp regulated,test
R2381 T9866 T9867 det the,transcription
R2382 T9867 T9865 nsubjpass transcription,regulated
R2383 T9868 T9867 prep of,transcription
R2384 T9869 T9870 compound mr,s
R2385 T9870 T9868 pobj s,of
R2386 T9871 T9870 punct -,s
R2387 T9872 T9865 auxpass is,regulated
R2388 T9873 T9865 advmod also,regulated
R2389 T9874 T9865 agent by,regulated
R2390 T9875 T9874 pobj Crx,by
R2391 T9876 T9865 prep in,regulated
R2392 T9877 T9878 det the,gland
R2393 T9878 T9876 pobj gland,in
R2394 T9879 T9878 amod pineal,gland
R2395 T9880 T9860 punct ", ",used
R2396 T9881 T9882 compound RT,PCR
R2397 T9882 T9884 compound PCR,analysis
R2398 T9883 T9882 punct -,PCR
R2399 T9884 T9860 nsubjpass analysis,used
R2400 T9885 T9860 auxpass was,used
R2401 T9886 T9887 aux to,compare
R2402 T9887 T9860 advcl compare,used
R2403 T9888 T9889 amod transcriptional,levels
R2421 T9906 T9905 compound Fig.,3C
R2422 T9907 T9905 punct ),3C
R2423 T9908 T9860 punct .,used
R2424 T9910 T9911 det The,results
R2425 T9911 T9912 nsubj results,revealed
R2426 T9913 T9914 mark that,downregulated
R2427 T9914 T9912 ccomp downregulated,revealed
R2428 T9915 T9916 compound mr,s
R2429 T9916 T9918 compound s,transcription
R2430 T9917 T9916 punct -,s
R2431 T9918 T9914 nsubjpass transcription,downregulated
R2432 T9919 T9914 auxpass was,downregulated
R2433 T9920 T9914 advmod significantly,downregulated
R2434 T9921 T9914 prep in,downregulated
R2435 T9922 T9923 det the,gland
R2436 T9923 T9921 pobj gland,in
R2437 T9924 T9925 nmod Crx,KO
R2438 T9925 T9923 nmod KO,gland
R2439 T9926 T9923 amod pineal,gland
R2440 T9927 T9912 punct .,revealed
R2441 T9929 T9930 advcl Taken,indicate
R2442 T9931 T9929 advmod together,Taken
R2443 T9932 T9930 punct ", ",indicate
R2444 T9933 T9934 det these,data
R2445 T9934 T9930 nsubj data,indicate
R2446 T9935 T9936 mark that,regulated
R2447 T9936 T9930 ccomp regulated,indicate
R2448 T9937 T9938 compound mr,s
R2449 T9938 T9940 compound s,transcription
R2450 T9939 T9938 punct -,s
R2451 T9940 T9936 nsubjpass transcription,regulated
R2452 T9941 T9936 auxpass is,regulated
R2453 T9942 T9936 agent by,regulated
R2454 T9943 T9942 pobj Crx,by
R2455 T9944 T9936 prep in,regulated
R2456 T9945 T9946 det the,photoreceptors
R2457 T9946 T9944 pobj photoreceptors,in
R2458 T9947 T9946 amod developing,photoreceptors
R2459 T9948 T9946 cc and,photoreceptors
R2460 T9949 T9950 amod pineal,gland
R2461 T9950 T9946 conj gland,photoreceptors
R2462 T9951 T9930 punct .,indicate
R2463 T9954 T9955 det T,o
R2464 T9955 T9956 nsubjpass o,u
R2465 T9957 T9955 prep f,o
R2466 T9960 T9961 amod t,h
R2500 T10033 T10031 pobj reporter,as
R2501 T10034 T10033 compound luciferase,reporter
R2502 T10035 T10036 punct (,3D
R2503 T10036 T10033 parataxis 3D,reporter
R2504 T10037 T10036 compound Fig.,3D
R2505 T10038 T10036 punct ", ",3D
R2506 T10039 T10036 appos Pro1.2k,3D
R2507 T10040 T10036 punct ),3D
R2508 T10041 T10029 cc and,gene
R2509 T10042 T10043 det the,vectors
R2510 T10043 T10029 conj vectors,gene
R2511 T10044 T10045 nmod Crx,Nrl
R2512 T10045 T10043 compound Nrl,vectors
R2513 T10046 T10045 punct ", ",Nrl
R2514 T10047 T10045 nmod Otx2,Nrl
R2515 T10048 T10045 punct ", ",Nrl
R2516 T10049 T10043 compound expression,vectors
R2517 T10050 T10026 punct ", ",fused
R2518 T10051 T10026 advmod respectively,fused
R2519 T10052 T10003 punct .,performed
R2520 T10054 T10055 det This,region
R2521 T10055 T10059 nsubj region,contains
R2522 T10056 T10057 nummod 1.2,kb
R2523 T10057 T10055 compound kb,region
R2524 T10058 T10057 punct -,kb
R2525 T10060 T10055 prep of,region
R2526 T10061 T10062 det the,sequence
R2527 T10062 T10060 pobj sequence,of
R2528 T10063 T10064 nmod mr,s
R2529 T10064 T10062 nmod s,sequence
R2530 T10065 T10064 punct -,s
R2531 T10066 T10062 amod upstream,sequence
R2532 T10067 T10068 nummod three,sequences
R2533 T10068 T10059 dobj sequences,contains
R2534 T10069 T10070 compound Crx,binding
R2535 T10070 T10068 compound binding,sequences
R2536 T10071 T10068 compound consensus,sequences
R2537 T10072 T10059 punct .,contains
R2538 T10074 T10075 mark As,shown
R2539 T10075 T10076 advcl shown,upregulated
R2540 T10077 T10075 prep in,shown
R2541 T10078 T10079 compound Fig.,3E
R2542 T10079 T10077 pobj 3E,in
R2543 T10080 T10076 punct ", ",upregulated
R2544 T10081 T10082 det the,activity
R2545 T10082 T10076 nsubjpass activity,upregulated
R2546 T10083 T10082 compound luciferase,activity
R2547 T10084 T10076 auxpass was,upregulated
R2548 T10085 T10076 advmod significantly,upregulated
R2549 T10086 T10087 advmod when,co-introduced
R2550 T10087 T10076 advcl co-introduced,upregulated
R2551 T10088 T10089 det the,vector
R2552 T10089 T10087 nsubjpass vector,co-introduced
R2553 T10090 T10089 nmod Crx,vector
R2554 T10091 T10090 cc or,Crx
R2555 T10092 T10090 conj Otx2,Crx
R2556 T10093 T10089 compound expression,vector
R2557 T10094 T10087 auxpass was,co-introduced
R2558 T10095 T10087 prep with,co-introduced
R2559 T10096 T10095 pobj Pro1.2k,with
R2560 T10097 T10087 prep into,co-introduced
R2561 T10098 T10099 compound HEK293T,cells
R2562 T10099 T10097 pobj cells,into
R2563 T10100 T10087 punct ", ",co-introduced
R2564 T10101 T10102 mark while,altered
R2565 T10102 T10087 advcl altered,co-introduced
R2566 T10103 T10104 det the,activity
R2567 T10104 T10102 nsubjpass activity,altered
R2568 T10105 T10104 compound luciferase,activity
R2569 T10106 T10102 auxpass was,altered
R2570 T10107 T10102 neg not,altered
R2571 T10108 T10109 advmod when,co-introduced
R2572 T10109 T10102 advcl co-introduced,altered
R2573 T10110 T10111 det the,vector
R2574 T10111 T10109 nsubjpass vector,co-introduced
R2575 T10112 T10111 compound Nrl,vector
R2576 T10113 T10111 compound expression,vector
R2577 T10114 T10109 auxpass was,co-introduced
R2578 T10115 T10076 punct .,upregulated
R2579 T10117 T10118 det A,report
R2580 T10118 T10120 nsubj report,suggested
R2581 T10119 T10118 amod previous,report
R2582 T10121 T10122 mark that,augmented
R2583 T10122 T10120 ccomp augmented,suggested
R2584 T10123 T10124 det the,activity
R2585 T10124 T10122 nsubjpass activity,augmented
R2586 T10125 T10124 amod transcriptional,activity
R2587 T10126 T10124 prep of,activity
R2588 T10127 T10126 pobj Crx,of
R2589 T10128 T10122 auxpass is,augmented
R2590 T10129 T10122 prep with,augmented
R2591 T10130 T10129 pobj Nrl,with
R2592 T10131 T10132 advmod when,used
R2593 T10132 T10122 advcl used,augmented
R2594 T10133 T10134 det the,promoter
R2595 T10134 T10132 nsubjpass promoter,used
R2596 T10135 T10134 compound rhodopsin,promoter
R2597 T10136 T10132 auxpass was,used
R2598 T10137 T10132 prep as,used
R2599 T10138 T10139 det a,gene
R2600 T10139 T10137 pobj gene,as
R2601 T10140 T10139 compound reporter,gene
R2602 T10141 T10142 punct [,6
R2603 T10142 T10120 parataxis 6,suggested
R2604 T10143 T10142 punct ],6
R2605 T10144 T10120 punct .,suggested
R2606 T10146 T10147 prep On,showed
R2607 T10148 T10149 det the,hand
R2608 T10149 T10146 pobj hand,On
R2609 T10150 T10149 amod other,hand
R2610 T10151 T10147 punct ", ",showed
R2611 T10152 T10153 poss our,data
R2612 T10153 T10147 nsubj data,showed
R2613 T10154 T10153 amod present,data
R2614 T10155 T10156 mark that,upregulated
R2615 T10156 T10147 ccomp upregulated,showed
R2616 T10157 T10158 det the,expression
R2617 T10158 T10156 nsubjpass expression,upregulated
R2618 T10159 T10158 compound luciferase,expression
R2619 T10160 T10158 compound gene,expression
R2620 T10161 T10156 auxpass was,upregulated
R2621 T10162 T10156 neg not,upregulated
R2622 T10163 T10164 advmod when,co-introduced
R2623 T10164 T10156 advcl co-introduced,upregulated
R2624 T10165 T10166 preconj both,Crx
R2625 T10166 T10167 nmod Crx,vectors
R2626 T10167 T10164 nsubjpass vectors,co-introduced
R2627 T10168 T10166 cc and,Crx
R2628 T10169 T10166 conj Nrl,Crx
R2629 T10170 T10167 compound expression,vectors
R2630 T10171 T10164 auxpass were,co-introduced
R2631 T10172 T10164 prep with,co-introduced
R2632 T10173 T10172 pobj Pro1.2k,with
R2633 T10174 T10156 prep compared,upregulated
R2634 T10175 T10174 prep to,compared
R2635 T10176 T10177 det the,activity
R2636 T10177 T10175 pobj activity,to
R2637 T10178 T10179 advmod when,introduced
R2638 T10179 T10177 relcl introduced,activity
R2639 T10180 T10181 det the,vector
R2640 T10181 T10179 nsubjpass vector,introduced
R2641 T10182 T10181 nmod Crx,vector
R2642 T10183 T10182 amod only,Crx
R2643 T10184 T10181 compound expression,vector
R2644 T10185 T10179 auxpass was,introduced
R2645 T10186 T10147 punct .,showed
R2646 T10188 T10189 nsubj This,be
R2647 T10190 T10189 aux may,be
R2648 T10191 T10189 prep due,be
R2649 T10192 T10191 pcomp to,due
R2650 T10193 T10194 compound cell,type
R2651 T10194 T10195 compound type,differences
R2652 T10195 T10191 pobj differences,due
R2653 T10196 T10197 mark because,used
R2654 T10197 T10189 advcl used,be
R2655 T10198 T10199 det a,type
R2656 T10199 T10197 nsubjpass type,used
R2657 T10200 T10199 compound cell,type
R2658 T10201 T10199 prep of,type
R2659 T10202 T10203 amod retinal,pineal
R2660 T10203 T10205 amod pineal,origin
R2661 T10204 T10203 punct /,pineal
R2662 T10205 T10201 pobj origin,of
R2663 T10206 T10197 auxpass was,used
R2664 T10207 T10197 neg not,used
R2665 T10208 T10197 prep in,used
R2666 T10209 T10210 poss our,assay
R2667 T10210 T10208 pobj assay,in
R2668 T10211 T10210 compound luciferase,assay
R2669 T10212 T10189 punct .,be
R2670 T10214 T10215 prep In,showed
R2671 T10216 T10214 pobj addition,In
R2672 T10217 T10215 punct ", ",showed
R2673 T10218 T10219 poss our,data
R2674 T10219 T10215 nsubj data,showed
R2675 T10220 T10219 amod present,data
R2676 T10221 T10222 mark that,transactivated
R2677 T10222 T10215 ccomp transactivated,showed
R2678 T10223 T10222 nsubj Otx2,transactivated
R2679 T10224 T10223 punct ", ",Otx2
R2680 T10225 T10226 dep which,reported
R2681 T10226 T10223 relcl reported,Otx2
R2682 T10227 T10226 auxpass is,reported
R2683 T10228 T10229 aux to,have
R2684 T10229 T10226 xcomp have,reported
R2685 T10230 T10231 det the,consensus
R2686 T10231 T10229 dobj consensus,have
R2687 T10232 T10231 amod same,consensus
R2688 T10233 T10231 compound binding,consensus
R2689 T10234 T10231 prep as,consensus
R2690 T10235 T10234 pobj Crx,as
R2691 T10236 T10222 punct ", ",transactivated
R2692 T10237 T10222 advmod also,transactivated
R2693 T10238 T10239 compound mr,s
R2694 T10239 T10241 compound s,expression
R2695 T10240 T10239 punct -,s
R2696 T10241 T10222 dobj expression,transactivated
R2697 T10242 T10215 punct .,showed
R2698 T10244 T10245 mark As,shown
R2699 T10245 T10246 advcl shown,correlates
R2700 T10247 T10245 prep in,shown
R2701 T10248 T10249 compound Fig.,2A
R2702 T10249 T10247 pobj 2A,in
R2703 T10250 T10251 punct –,F
R2704 T10251 T10249 prep F,2A
R2705 T10252 T10246 punct ", ",correlates
R2706 T10253 T10254 det the,pattern
R2707 T10254 T10246 nsubj pattern,correlates
R2708 T10255 T10254 compound expression,pattern
R2709 T10256 T10254 prep of,pattern
R2710 T10257 T10258 compound mr,s
R2711 T10258 T10256 pobj s,of
R2712 T10259 T10258 punct -,s
R2713 T10260 T10246 prep with,correlates
R2714 T10261 T10260 pobj that,with
R2715 T10262 T10261 prep of,that
R2716 T10263 T10262 pobj Crx,of
R2717 T10264 T10246 punct .,correlates
R2718 T10266 T10267 prep In,detected
R2719 T10268 T10266 pobj contrast,In
R2720 T10269 T10267 punct ", ",detected
R2721 T10270 T10271 det the,transcripts
R2722 T10271 T10267 nsubjpass transcripts,detected
R2723 T10272 T10271 prep of,transcripts
R2724 T10273 T10272 pobj Otx2,of
R2725 T10274 T10267 auxpass are,detected
R2726 T10275 T10267 advmod mainly,detected
R2727 T10276 T10267 prep in,detected
R2728 T10277 T10278 det the,layer
R2729 T10278 T10276 pobj layer,in
R2730 T10279 T10278 compound photoreceptor,layer
R2731 T10280 T10267 prep at,detected
R2732 T10281 T10282 amod embryonic,stages
R2733 T10282 T10280 pobj stages,at
R2734 T10283 T10267 punct .,detected
R2735 T10285 T10286 advmod Therefore,concluded
R2736 T10287 T10286 punct ", ",concluded
R2737 T10288 T10286 nsubj we,concluded
R2738 T10289 T10290 mark that,regulated
R2739 T10290 T10286 ccomp regulated,concluded
R2740 T10291 T10292 compound mr,s
R2741 T10292 T10294 compound s,transcription
R2742 T10293 T10292 punct -,s
R2743 T10294 T10290 nsubjpass transcription,regulated
R2744 T10295 T10290 auxpass is,regulated
R2745 T10296 T10290 advmod directly,regulated
R2746 T10297 T10298 advmod mainly,by
R2747 T10298 T10290 agent by,regulated
R2748 T10299 T10298 pobj Crx,by
R2749 T10300 T10286 punct .,concluded
R2750 T10302 T10303 nsubj We,constructed
R2751 T10304 T10303 advmod also,constructed
R2752 T10305 T10306 compound reporter,vectors
R2753 T10306 T10303 dobj vectors,constructed
R2754 T10307 T10308 prep in,introduced
R2755 T10308 T10306 relcl introduced,vectors
R2756 T10309 T10307 pobj which,in
R2757 T10310 T10308 nsubjpass mutations,introduced
R2758 T10311 T10308 auxpass were,introduced
R2759 T10312 T10308 prep at,introduced
R2760 T10313 T10314 det the,sites
R2761 T10314 T10312 pobj sites,at
R2762 T10315 T10314 nummod three,sites
R2763 T10316 T10317 compound Crx,binding
R2764 T10317 T10314 compound binding,sites
R2765 T10318 T10319 punct (,mut1259
R2766 T10319 T10303 parataxis mut1259,constructed
R2767 T10320 T10321 compound Fig.,3D
R2768 T10321 T10319 dep 3D,mut1259
R2769 T10322 T10319 punct ", ",mut1259
R2770 T10323 T10319 punct ", ",mut1259
R2771 T10324 T10319 appos mut198,mut1259
R2772 T10325 T10319 punct ", ",mut1259
R2773 T10326 T10319 appos mut72,mut1259
R2774 T10327 T10319 punct ", ",mut1259
R2775 T10328 T10329 compound mut,all
R2776 T10329 T10319 appos all,mut1259
R2777 T10330 T10319 punct ),mut1259
R2778 T10331 T10303 punct .,constructed
R2779 T10333 T10334 advmod Then,co-introduced
R2780 T10335 T10336 det the,vector
R2781 T10336 T10334 nsubjpass vector,co-introduced
R2782 T10337 T10336 compound Crx,vector
R2783 T10338 T10336 compound expression,vector
R2784 T10339 T10334 auxpass was,co-introduced
R2785 T10340 T10334 prep with,co-introduced
R2786 T10341 T10340 pobj mut1259,with
R2787 T10342 T10341 punct ", ",mut1259
R2788 T10343 T10341 conj mut198,mut1259
R2789 T10344 T10343 punct ", ",mut198
R2790 T10345 T10343 conj mut72,mut198
R2791 T10346 T10345 cc and,mut72
R2792 T10347 T10348 compound mut,all
R2793 T10348 T10345 conj all,mut72
R2794 T10349 T10334 punct ", ",co-introduced
R2795 T10350 T10334 advmod respectively,co-introduced
R2796 T10351 T10352 punct (,3F
R2797 T10352 T10334 parataxis 3F,co-introduced
R2798 T10353 T10352 compound Fig.,3F
R2799 T10354 T10352 punct ),3F
R2800 T10355 T10334 punct .,co-introduced
R2801 T10357 T10358 det The,activity
R2802 T10358 T10360 nsubjpass activity,reduced
R2803 T10359 T10358 compound transactivaton,activity
R2804 T10361 T10358 prep by,activity
R2805 T10362 T10361 pobj Crx,by
R2806 T10363 T10360 auxpass was,reduced
R2807 T10364 T10360 advmod clearly,reduced
R2808 T10365 T10366 advmod when,co-introduced
R2809 T10366 T10360 advcl co-introduced,reduced
R2810 T10367 T10366 nsubjpass mut198,co-introduced
R2811 T10368 T10367 cc or,mut198
R2812 T10369 T10367 conj mut72,mut198
R2813 T10370 T10366 auxpass was,co-introduced
R2814 T10371 T10360 punct .,reduced
R2815 T10373 T10374 prep On,altered
R2816 T10375 T10376 det the,hand
R2817 T10376 T10373 pobj hand,On
R2818 T10377 T10376 amod other,hand
R2819 T10378 T10374 punct ", ",altered
R2820 T10379 T10380 advmod when,co-transfected
R2821 T10380 T10374 advcl co-transfected,altered
R2822 T10381 T10380 nsubjpass mut1259,co-transfected
R2823 T10382 T10380 auxpass was,co-transfected
R2824 T10383 T10374 punct ", ",altered
R2825 T10384 T10385 det the,activity
R2826 T10385 T10374 nsubjpass activity,altered
R2827 T10386 T10385 compound transactivation,activity
R2828 T10387 T10385 prep by,activity
R2829 T10388 T10387 pobj Crx,by
R2830 T10389 T10374 auxpass was,altered
R2831 T10390 T10374 neg not,altered
R2832 T10391 T10374 punct .,altered
R2833 T10393 T10394 det These,results
R2834 T10394 T10395 nsubj results,suggest
R2835 T10396 T10397 mark that,are
R2836 T10397 T10395 ccomp are,suggest
R2837 T10398 T10399 det the,sites
R2838 T10399 T10397 nsubj sites,are
R2839 T10400 T10401 compound Crx,binding
R2840 T10401 T10399 compound binding,sites
R2841 T10402 T10403 nummod 72,bp
R2842 T10403 T10405 npadvmod bp,upstream
R2843 T10404 T10403 punct -,bp
R2844 T10405 T10399 advmod upstream,sites
R2845 T10406 T10403 cc and,bp
R2846 T10407 T10408 nummod 198,bp
R2847 T10408 T10403 conj bp,bp
R2848 T10409 T10408 punct -,bp
R2849 T10410 T10405 prep from,upstream
R2850 T10411 T10412 det the,site
R2851 T10412 T10410 pobj site,from
R2852 T10413 T10412 compound transcription,site
R2853 T10414 T10412 compound initiation,site
R2854 T10415 T10397 acomp crucial,are
R2855 T10416 T10415 prep for,crucial
R2856 T10417 T10418 det the,regulation
R2857 T10418 T10416 pobj regulation,for
R2858 T10419 T10418 amod direct,regulation
R2859 T10420 T10418 prep of,regulation
R2860 T10421 T10422 compound mr,s
R2861 T10422 T10424 compound s,transcription
R2862 T10423 T10422 punct -,s
R2863 T10424 T10420 pobj transcription,of
R2864 T10425 T10418 prep by,regulation
R2865 T10426 T10425 pobj Crx,by
R2866 T10427 T10395 punct .,suggest
R2867 T12462 T12463 compound Self,association
R2868 T12464 T12463 punct -,association
R2869 T12465 T12463 prep of,association
R2870 T12466 T12467 compound mr,s
R2871 T12467 T12469 compound s,protein
R2872 T12468 T12467 punct -,s
R2873 T12469 T12465 pobj protein,of
R2874 T12471 T12472 compound SAM,domains
R2875 T12472 T12473 nsubjpass domains,known
R2876 T12474 T12473 auxpass are,known
R2877 T12475 T12476 aux to,function
R2878 T12476 T12473 xcomp function,known
R2879 T12477 T12476 prep as,function
R2880 T12478 T12479 compound protein,protein
R2881 T12479 T12481 compound protein,modules
R2882 T12480 T12479 punct -,protein
R2883 T12481 T12477 pobj modules,as
R2884 T12482 T12481 compound interaction,modules
R2885 T12483 T12484 punct [,15
R2886 T12484 T12473 parataxis 15,known
R2887 T12485 T12486 punct -,17
R2888 T12486 T12484 prep 17,15
R2889 T12487 T12484 punct ],15
R2890 T12488 T12473 punct .,known
R2891 T12490 T12491 mark Although,bind
R2892 T12491 T12495 advcl bind,reported
R2893 T12492 T12493 compound SAM,domains
R2894 T12493 T12491 nsubj domains,bind
R2895 T12494 T12491 aux can,bind
R2896 T12496 T12491 prep to,bind
R2897 T12497 T12498 amod various,proteins
R2898 T12498 T12496 pobj proteins,to
R2899 T12499 T12500 amod non-SAM,domain
R2900 T12500 T12501 npadvmod domain,containing
R2901 T12501 T12498 amod containing,proteins
R2902 T12502 T12501 punct -,containing
R2903 T12503 T12495 punct ", ",reported
R2904 T12504 T12505 amod many,interactions
R2905 T12505 T12495 nsubjpass interactions,reported
R2906 T12506 T12505 nmod homo-SAM,interactions
R2907 T12507 T12506 cc and,homo-SAM
R2908 T12508 T12506 conj hetero-SAM,homo-SAM
R2909 T12509 T12505 compound domain,interactions
R2910 T12510 T12495 aux have,reported
R2911 T12511 T12495 auxpass been,reported
R2912 T12512 T12495 punct .,reported
R2913 T12514 T12515 aux To,investigate
R2914 T12515 T12516 advcl investigate,performed
R2915 T12517 T12518 mark whether,function
R2916 T12518 T12515 ccomp function,investigate
R2917 T12519 T12520 det the,domain
R2918 T12520 T12518 nsubj domain,function
R2919 T12521 T12520 compound SAM,domain
R2920 T12522 T12520 prep of,domain
R2921 T12523 T12524 det the,protein
R2922 T12524 T12522 pobj protein,of
R2923 T12525 T12526 compound mr,s
R2924 T12526 T12524 compound s,protein
R2925 T12527 T12526 punct -,s
R2926 T12528 T12518 aux can,function
R2927 T12529 T12518 advmod also,function
R2928 T12530 T12518 prep as,function
R2929 T12531 T12532 det a,module
R2930 T12532 T12530 pobj module,as
R2931 T12533 T12534 compound protein,protein
R2932 T12534 T12532 compound protein,module
R2933 T12535 T12534 punct -,protein
R2934 T12536 T12532 compound interaction,module
R2935 T12537 T12516 punct ", ",performed
R2936 T12538 T12516 nsubj we,performed
R2937 T12539 T12540 nmod yeast,screening
R2938 T12540 T12516 dobj screening,performed
R2939 T12541 T12542 nummod two,hybrid
R2940 T12542 T12540 compound hybrid,screening
R2941 T12543 T12542 punct -,hybrid
R2942 T12544 T12516 advcl using,performed
R2943 T12545 T12546 amod full,length
R2944 T12546 T12548 compound length,protein
R2945 T12547 T12546 punct -,length
R2946 T12548 T12544 dobj protein,using
R2947 T12549 T12550 compound mr,s
R2948 T12550 T12548 compound s,protein
R2949 T12551 T12550 punct -,s
R2950 T12552 T12544 prep as,using
R2951 T12553 T12554 det the,bait
R2952 T12554 T12552 pobj bait,as
R2953 T12555 T12516 punct .,performed
R2954 T12557 T12558 advcl Using,screened
R2955 T12559 T12560 det this,bait
R2956 T12560 T12557 dobj bait,Using
R2957 T12561 T12558 punct ", ",screened
R2958 T12562 T12558 nsubj we,screened
R2959 T12563 T12564 det the,library
R2960 T12564 T12558 dobj library,screened
R2961 T12565 T12566 amod transcriptional,activator
R2962 T12566 T12567 compound activator,protein
R2963 T12567 T12564 compound protein,library
R2964 T12568 T12567 compound fusion,protein
R2965 T12569 T12570 prep in,fused
R2966 T12570 T12564 advcl fused,library
R2967 T12571 T12569 pobj which,in
R2968 T12572 T12573 nmod mouse,cDNAs
R2969 T12573 T12570 nsubjpass cDNAs,fused
R2970 T12574 T12573 nmod P0,cDNAs
R2971 T12575 T12576 punct -,P3
R2972 T12576 T12574 prep P3,P0
R2973 T12577 T12573 amod retinal,cDNAs
R2974 T12578 T12570 auxpass were,fused
R2975 T12579 T12570 prep to,fused
R2976 T12580 T12581 det the,domain
R2977 T12581 T12579 pobj domain,to
R2978 T12582 T12581 compound GAL4,domain
R2979 T12583 T12581 compound activation,domain
R2980 T12584 T12558 punct .,screened
R2981 T12586 T12587 det The,clones
R2982 T12587 T12591 nsubj clones,were
R2983 T12588 T12589 advmod most,frequent
R2984 T12589 T12587 amod frequent,clones
R2985 T12590 T12587 amod positive,clones
R2986 T12592 T12593 punct (,28
R2987 T12593 T12587 parataxis 28,clones
R2988 T12594 T12593 quantmod 5,28
R2989 T12595 T12593 quantmod out,28
R2990 T12596 T12593 quantmod of,28
R2991 T12597 T12593 punct ),28
R2992 T12598 T12599 compound cDNA,fragments
R2993 T12599 T12591 attr fragments,were
R2994 T12600 T12599 acl containing,fragments
R2995 T12601 T12602 det the,domain
R2996 T12602 T12600 dobj domain,containing
R2997 T12603 T12602 compound SAM,domain
R2998 T12604 T12602 prep of,domain
R2999 T12605 T12606 compound mr,s
R3000 T12606 T12604 pobj s,of
R3001 T12607 T12606 punct -,s
R3002 T12608 T12609 punct (,4A
R3003 T12609 T12591 parataxis 4A,were
R3004 T12610 T12609 compound Fig.,4A
R3005 T12611 T12609 punct ),4A
R3006 T12612 T12591 punct .,were
R3007 T12614 T12615 det This,result
R3008 T12615 T12616 nsubj result,suggests
R3009 T12617 T12616 advmod strongly,suggests
R3010 T12618 T12619 mark that,associates
R3011 T12619 T12616 ccomp associates,suggests
R3012 T12620 T12621 compound mr,s
R3013 T12621 T12623 compound s,protein
R3014 T12622 T12621 punct -,s
R3015 T12623 T12619 nsubj protein,associates
R3016 T12624 T12619 dep self,associates
R3017 T12625 T12619 punct -,associates
R3018 T12626 T12619 prep through,associates
R3019 T12627 T12628 compound SAM,domain
R3020 T12628 T12629 npadvmod domain,containing
R3021 T12629 T12631 amod containing,regions
R3022 T12630 T12629 punct -,containing
R3023 T12631 T12626 pobj regions,through
R3024 T12632 T12616 punct .,suggests
R3025 T12634 T12635 nsubj We,tested
R3026 T12636 T12635 advmod then,tested
R3027 T12637 T12635 advmod directly,tested
R3028 T12638 T12639 det this,association
R3029 T12639 T12635 dobj association,tested
R3030 T12640 T12639 compound self,association
R3031 T12641 T12639 punct -,association
R3032 T12642 T12639 prep of,association
R3033 T12643 T12644 compound mr,s
R3034 T12644 T12646 compound s,protein
R3035 T12645 T12644 punct -,s
R3036 T12646 T12642 pobj protein,of
R3037 T12647 T12635 prep in,tested
R3038 T12648 T12647 pobj yeast,in
R3039 T12649 T12635 punct .,tested
R3040 T12651 T12652 nsubj We,fused
R3041 T12653 T12654 amod full,length
R3042 T12654 T12656 nmod length,portions
R3043 T12655 T12654 punct -,length
R3044 T12656 T12652 dobj portions,fused
R3045 T12657 T12654 cc or,length
R3046 T12658 T12654 conj truncated,length
R3047 T12659 T12656 prep of,portions
R3048 T12660 T12661 det the,protein
R3049 T12661 T12659 pobj protein,of
R3050 T12662 T12663 compound mr,s
R3051 T12663 T12661 compound s,protein
R3052 T12664 T12663 punct -,s
R3053 T12665 T12652 prep to,fused
R3054 T12666 T12667 det the,domain
R3055 T12667 T12665 pobj domain,to
R3056 T12668 T12669 npadvmod DNA,binding
R3057 T12669 T12667 amod binding,domain
R3058 T12670 T12669 punct -,binding
R3059 T12671 T12667 prep of,domain
R3060 T12672 T12673 det the,factor
R3061 T12673 T12671 pobj factor,of
R3062 T12674 T12673 compound yeast,factor
R3063 T12675 T12673 compound transcription,factor
R3064 T12676 T12673 appos GAL4,factor
R3065 T12677 T12678 aux to,make
R3066 T12678 T12652 advcl make,fused
R3067 T12679 T12680 compound bait,constructs
R3068 T12680 T12678 dobj constructs,make
R3069 T12681 T12652 punct .,fused
R3070 T12683 T12684 nsubj We,fused
R3071 T12685 T12686 amod full,length
R3072 T12686 T12688 nmod length,portions
R3073 T12687 T12686 punct -,length
R3074 T12688 T12684 dobj portions,fused
R3075 T12689 T12686 cc or,length
R3076 T12690 T12686 conj truncated,length
R3077 T12691 T12688 prep of,portions
R3078 T12692 T12693 det the,protein
R3079 T12693 T12691 pobj protein,of
R3080 T12694 T12695 compound mr,s
R3081 T12695 T12693 compound s,protein
R3082 T12696 T12695 punct -,s
R3083 T12697 T12684 prep to,fused
R3084 T12698 T12699 det the,domain
R3085 T12699 T12697 pobj domain,to
R3086 T12700 T12701 nmod GAL4,activation
R3087 T12701 T12699 compound activation,domain
R3088 T12702 T12701 amod transcriptional,activation
R3089 T12703 T12704 aux to,make
R3090 T12704 T12684 advcl make,fused
R3091 T12705 T12706 compound prey,constructs
R3092 T12706 T12704 dobj constructs,make
R3093 T12707 T12708 punct (,4B
R3094 T12708 T12684 parataxis 4B,fused
R3095 T12709 T12708 compound Fig.,4B
R3096 T12710 T12708 punct ),4B
R3097 T12711 T12684 punct .,fused
R3098 T12713 T12714 det These,constructs
R3099 T12714 T12715 nsubjpass constructs,transformed
R3100 T12716 T12715 auxpass were,transformed
R3101 T12717 T12715 prep into,transformed
R3102 T12718 T12717 pobj yeast,into
R3103 T12719 T12720 dep that,contain
R3104 T12720 T12718 relcl contain,yeast
R3105 T12721 T12722 det a,transgene
R3106 T12722 T12720 dobj transgene,contain
R3107 T12723 T12722 prep with,transgene
R3108 T12724 T12725 compound GAL4,binding
R3109 T12725 T12726 compound binding,sites
R3110 T12726 T12723 pobj sites,with
R3111 T12727 T12726 advmod upstream,sites
R3112 T12728 T12727 prep of,upstream
R3113 T12729 T12730 det the,gene
R3114 T12730 T12728 pobj gene,of
R3115 T12731 T12730 compound lacZ,gene
R3116 T12732 T12715 punct .,transformed
R3117 T12734 T12735 nsubj We,found
R3118 T12736 T12737 mark that,induced
R3119 T12737 T12735 ccomp induced,found
R3120 T12738 T12739 det the,construct
R3121 T12739 T12737 nsubj construct,induced
R3122 T12740 T12741 amod full,length
R3123 T12741 T12739 compound length,construct
R3124 T12742 T12741 punct -,length
R3125 T12743 T12744 compound mr,s
R3126 T12744 T12739 compound s,construct
R3127 T12745 T12744 punct -,s
R3128 T12746 T12739 compound bait,construct
R3129 T12747 T12748 compound lacZ,expression
R3130 T12748 T12737 dobj expression,induced
R3131 T12749 T12737 prep with,induced
R3132 T12750 T12751 det the,construct
R3133 T12751 T12749 pobj construct,with
R3134 T12752 T12753 amod full,length
R3135 T12753 T12751 compound length,construct
R3136 T12754 T12753 punct -,length
R3137 T12755 T12756 compound mr,s
R3138 T12756 T12751 compound s,construct
R3139 T12757 T12756 punct -,s
R3140 T12758 T12751 compound prey,construct
R3141 T12759 T12760 punct (,full
R3142 T12760 T12735 parataxis full,found
R3143 T12761 T12762 compound Fig.,4B
R3144 T12762 T12760 dep 4B,full
R3145 T12763 T12760 punct ", ",full
R3146 T12764 T12760 amod full,full
R3147 T12765 T12760 punct ×,full
R3148 T12766 T12760 punct ),full
R3149 T12767 T12735 punct .,found
R3150 T12769 T12770 det The,stretch
R3151 T12770 T12780 nsubj stretch,activate
R3152 T12771 T12772 nmod N,terminus
R3153 T12772 T12770 nmod terminus,stretch
R3154 T12773 T12772 punct -,terminus
R3155 T12774 T12770 nummod 400,stretch
R3156 T12775 T12776 nmod amino,acid
R3157 T12776 T12770 nmod acid,stretch
R3158 T12777 T12776 punct (,acid
R3159 T12778 T12776 appos aa,acid
R3160 T12779 T12770 punct ),stretch
R3161 T12781 T12770 prep of,stretch
R3162 T12782 T12783 compound mr,s
R3163 T12783 T12781 pobj s,of
R3164 T12784 T12783 punct -,s
R3165 T12785 T12770 punct ", ",stretch
R3166 T12786 T12787 dep which,contain
R3167 T12787 T12770 relcl contain,stretch
R3168 T12788 T12787 aux does,contain
R3169 T12789 T12787 neg not,contain
R3170 T12790 T12791 det a,domain
R3171 T12791 T12787 dobj domain,contain
R3172 T12792 T12791 compound SAM,domain
R3173 T12793 T12780 punct ", ",activate
R3174 T12794 T12780 aux does,activate
R3175 T12795 T12780 advmod weakly,activate
R3176 T12796 T12780 dobj transcription,activate
R3177 T12797 T12796 prep of,transcription
R3178 T12798 T12797 pobj lacZ,of
R3179 T12799 T12800 punct (,N
R3180 T12800 T12780 parataxis N,activate
R3181 T12801 T12802 compound Fig.,4B
R3182 T12802 T12800 dep 4B,N
R3183 T12803 T12800 punct ", ",N
R3184 T12804 T12800 amod full,N
R3185 T12805 T12800 punct ×,N
R3186 T12806 T12800 punct ),N
R3187 T12807 T12780 punct .,activate
R3188 T12809 T12810 det The,stretch
R3189 T12810 T12816 nsubj stretch,was
R3190 T12811 T12812 nmod N,terminus
R3191 T12812 T12810 nmod terminus,stretch
R3192 T12813 T12812 punct -,terminus
R3193 T12814 T12815 nummod 400,aa
R3194 T12815 T12810 compound aa,stretch
R3195 T12817 T12810 prep of,stretch
R3196 T12818 T12819 compound mr,s
R3197 T12819 T12817 pobj s,of
R3198 T12820 T12819 punct -,s
R3199 T12821 T12816 acomp able,was
R3200 T12822 T12823 aux to,induce
R3201 T12823 T12821 xcomp induce,able
R3202 T12824 T12823 dobj transcription,induce
R3203 T12825 T12824 prep of,transcription
R3204 T12826 T12825 pobj lacZ,of
R3205 T12827 T12823 advmod weakly,induce
R3206 T12828 T12823 prep with,induce
R3207 T12829 T12830 det the,stretch
R3208 T12830 T12828 pobj stretch,with
R3209 T12831 T12830 amod same,stretch
R3210 T12832 T12833 nmod N,terminus
R3211 T12833 T12830 nmod terminus,stretch
R3212 T12834 T12833 punct -,terminus
R3213 T12835 T12836 nummod 400,aa
R3214 T12836 T12830 compound aa,stretch
R3215 T12837 T12830 prep of,stretch
R3216 T12838 T12839 compound mr,s
R3217 T12839 T12837 pobj s,of
R3218 T12840 T12839 punct -,s
R3219 T12841 T12842 punct (,N
R3220 T12842 T12816 parataxis N,was
R3221 T12843 T12844 compound Fig.,4B
R3222 T12844 T12842 dep 4B,N
R3223 T12845 T12842 punct ", ",N
R3224 T12846 T12842 nmod N,N
R3225 T12847 T12842 punct ×,N
R3226 T12848 T12842 punct ),N
R3227 T12849 T12816 punct .,was
R3228 T12851 T12852 mark Although,activates
R3229 T12852 T12864 advcl activates,observed
R3230 T12853 T12854 det the,protein
R3231 T12854 T12852 nsubj protein,activates
R3232 T12855 T12856 nmod N,terminus
R3233 T12856 T12854 nmod terminus,protein
R3234 T12857 T12856 punct -,terminus
R3235 T12858 T12859 nummod 400,aa
R3236 T12859 T12854 compound aa,protein
R3237 T12860 T12861 compound mr,s
R3238 T12861 T12854 compound s,protein
R3239 T12862 T12861 punct -,s
R3240 T12863 T12852 advmod weakly,activates
R3241 T12865 T12866 compound lacZ,transcription
R3242 T12866 T12852 dobj transcription,activates
R3243 T12867 T12852 prep with,activates
R3244 T12868 T12869 det the,portion
R3245 T12869 T12867 pobj portion,with
R3246 T12870 T12869 amod same,portion
R3247 T12871 T12872 compound N,terminus
R3248 T12872 T12869 compound terminus,portion
R3249 T12873 T12872 punct -,terminus
R3250 T12874 T12864 punct ", ",observed
R3251 T12875 T12876 det a,activation
R3252 T12876 T12864 nsubjpass activation,observed
R3253 T12877 T12878 advmod much,stronger
R3254 T12878 T12876 amod stronger,activation
R3255 T12879 T12876 prep of,activation
R3256 T12880 T12881 compound lacZ,expression
R3257 T12881 T12879 pobj expression,of
R3258 T12882 T12864 auxpass was,observed
R3259 T12883 T12864 prep with,observed
R3260 T12884 T12885 det a,portion
R3261 T12885 T12883 pobj portion,with
R3262 T12886 T12887 compound C,terminus
R3263 T12887 T12885 compound terminus,portion
R3264 T12888 T12887 punct -,terminus
R3265 T12889 T12885 acl encoding,portion
R3266 T12890 T12891 det the,stretch
R3267 T12891 T12889 dobj stretch,encoding
R3268 T12892 T12893 nummod 391,aa
R3269 T12893 T12891 compound aa,stretch
R3270 T12894 T12895 punct –,542
R3271 T12895 T12892 prep 542,391
R3272 T12896 T12891 prep of,stretch
R3273 T12897 T12898 compound mr,s
R3274 T12898 T12896 pobj s,of
R3275 T12899 T12898 punct -,s
R3276 T12900 T12901 punct (,C
R3277 T12901 T12864 parataxis C,observed
R3278 T12902 T12903 compound Fig.,4B
R3279 T12903 T12901 dep 4B,C
R3280 T12904 T12901 punct ", ",C
R3281 T12905 T12901 amod full,C
R3282 T12906 T12901 punct ×,C
R3283 T12907 T12901 punct ", ",C
R3284 T12908 T12909 nmod C,C
R3285 T12909 T12901 appos C,C
R3286 T12910 T12909 punct ×,C
R3287 T12911 T12901 punct ),C
R3288 T12912 T12864 punct .,observed
R3289 T12914 T12915 poss Our,assay
R3290 T12915 T12917 nsubj assay,indicated
R3291 T12916 T12915 compound GAL4,assay
R3292 T12918 T12919 mark that,was
R3294 T12920 T12921 det the,signal
R3295 T12921 T12919 nsubj signal,was
R3296 T12922 T12923 advmod when,was
R3298 T12924 T12925 det the,s
R3299 T12925 T12923 nsubj s,was
R3300 T12926 T12927 amod full,length
R3301 T12927 T12925 compound length,s
R3302 T12928 T12927 punct -,length
R3303 T12929 T12925 compound mr,s
R3304 T12930 T12925 punct -,s
R3319 T12945 T12944 compound SAM,domains
R3320 T12946 T12942 auxpass were,used
R3321 T12947 T12917 punct .,indicated
R3322 T12949 T12950 nsubj This,reflect
R3323 T12951 T12950 aux may,reflect
R3324 T12952 T12950 advmod simply,reflect
R3325 T12953 T12954 det the,tendency
R3326 T12954 T12950 dobj tendency,reflect
R3327 T12955 T12956 mark for,enter
R3328 T12956 T12954 advcl enter,tendency
R3329 T12957 T12958 det the,proteins
R3330 T12958 T12956 nsubj proteins,enter
R3331 T12959 T12958 amod small,proteins
R3332 T12960 T12958 compound fusion,proteins
R3333 T12961 T12956 aux to,enter
R3334 T12962 T12963 det the,nucleus
R3335 T12963 T12956 dobj nucleus,enter
R3336 T12964 T12963 compound yeast,nucleus
R3337 T12965 T12956 cc and,enter
R3338 T12966 T12956 conj occupy,enter
R3339 T12967 T12968 compound GAL4,binding
R3340 T12968 T12969 compound binding,sites
R3341 T12969 T12966 dobj sites,occupy
R3342 T12970 T12950 punct .,reflect
R3343 T12972 T12973 advmod Alternatively,be
R3344 T12974 T12973 punct ", ",be
R3345 T12975 T12976 det the,domain
R3346 T12976 T12973 nsubj domain,be
R3347 T12977 T12976 compound SAM,domain
R3348 T12978 T12973 aux may,be
R3349 T12979 T12980 advmod less,accessible
R3350 T12980 T12973 acomp accessible,be
R3351 T12981 T12980 prep for,accessible
R3352 T12982 T12981 pobj interaction,for
R3353 T12983 T12973 prep in,be
R3354 T12984 T12985 det the,context
R3355 T12985 T12983 pobj context,in
R3356 T12986 T12987 amod full,length
R3357 T12987 T12985 compound length,context
R3358 T12988 T12987 punct -,length
R3359 T12989 T12985 compound protein,context
R3360 T12990 T12991 mark as,reported
R3361 T12991 T12973 advcl reported,be
R3362 T12992 T12991 advmod previously,reported
R3363 T12993 T12994 punct [,39
R3364 T12994 T12973 parataxis 39,be
R3365 T12995 T12994 punct ],39
R3366 T12996 T12973 punct .,be
R3372 T13005 T13003 prep of,association
R3373 T13006 T13007 det the,protein
R3374 T13007 T13005 pobj protein,of
R3375 T13008 T13009 compound mr,s
R3376 T13009 T13007 compound s,protein
R3377 T13010 T13009 punct -,s
R3378 T13011 T13000 prep in,confirm
R3379 T13012 T13013 amod mammalian,cells
R3380 T13013 T13011 pobj cells,in
R3381 T13014 T13001 punct ", ",performed
R3382 T13015 T13001 nsubj we,performed
R3383 T13016 T13001 advmod next,performed
R3384 T13017 T13018 compound co-immunoprecipitation,studies
R3385 T13018 T13001 dobj studies,performed
R3386 T13019 T13001 prep in,performed
R3387 T13020 T13021 compound HEK293T,cells
R3388 T13021 T13019 pobj cells,in
R3389 T13022 T13001 prep by,performed
R3390 T13023 T13022 pobj co-transfection,by
R3391 T13024 T13023 prep of,co-transfection
R3392 T13025 T13026 npadvmod HA,tagged
R3393 T13026 T13028 amod tagged,s
R3394 T13027 T13026 punct -,tagged
R3395 T13028 T13024 pobj s,of
R3396 T13029 T13030 amod full,length
R3397 T13030 T13032 dep length,truncated
R3398 T13031 T13030 punct -,length
R3399 T13032 T13028 amod truncated,s
R3400 T13033 T13032 punct /,truncated
R3401 T13034 T13028 compound mr,s
R3402 T13035 T13028 punct -,s
R3403 T13036 T13028 cc and,s
R3404 T13037 T13038 npadvmod Flag,tagged
R3405 T13038 T13040 amod tagged,s
R3406 T13039 T13038 punct -,tagged
R3407 T13040 T13028 conj s,s
R3408 T13041 T13042 amod full,length
R3409 T13042 T13044 dep length,truncated
R3410 T13043 T13042 punct -,length
R3411 T13044 T13040 amod truncated,s
R3412 T13045 T13044 punct /,truncated
R3413 T13046 T13040 compound mr,s
R3414 T13047 T13040 punct -,s
R3415 T13048 T13049 punct (,5A
R3416 T13049 T13001 parataxis 5A,performed
R3417 T13050 T13049 compound Fig.,5A
R3418 T13051 T13049 punct ),5A
R3419 T13052 T13001 punct .,performed
R3420 T13054 T13055 prep As,constructed
R3421 T13056 T13057 det a,control
R3422 T13057 T13054 pobj control,As
R3423 T13058 T13057 amod negative,control
R3424 T13059 T13055 punct ", ",constructed
R3425 T13060 T13055 nsubj we,constructed
R3426 T13061 T13062 npadvmod Flag,tagged
R3427 T13062 T13064 amod tagged,hedgehog
R3428 T13063 T13062 punct -,tagged
R3429 T13064 T13055 dobj hedgehog,constructed
R3430 T13065 T13064 amod Sonic,hedgehog
R3431 T13066 T13064 punct (,hedgehog
R3432 T13067 T13064 appos Shh,hedgehog
R3433 T13068 T13055 punct ),constructed
R3434 T13069 T13070 punct (,2
R3435 T13070 T13055 parataxis 2,constructed
R3436 T13071 T13070 nmod lane,2
R3437 T13072 T13070 cc and,2
R3438 T13073 T13070 conj 7,2
R3439 T13074 T13070 punct ),2
R3440 T13075 T13055 punct .,constructed
R3441 T13077 T13078 prep In,co-immunoprecipitated
R3442 T13079 T13077 pobj accordance,In
R3443 T13080 T13079 prep with,accordance
R3444 T13081 T13082 det the,result
R3445 T13082 T13080 pobj result,with
R3446 T13083 T13082 prep of,result
R3447 T13084 T13085 det the,assay
R3448 T13085 T13083 pobj assay,of
R3449 T13086 T13085 nmod yeast,assay
R3450 T13087 T13088 nummod two,hybrid
R3451 T13088 T13085 compound hybrid,assay
R3452 T13089 T13088 punct -,hybrid
R3453 T13090 T13085 compound GAL4,assay
R3454 T13091 T13078 punct ", ",co-immunoprecipitated
R3455 T13092 T13093 npadvmod HA,tagged
R3456 T13093 T13095 amod tagged,s
R3457 T13094 T13093 punct -,tagged
R3458 T13095 T13078 nsubjpass s,co-immunoprecipitated
R3459 T13096 T13097 amod full,length
R3460 T13097 T13095 compound length,s
R3461 T13098 T13097 punct -,length
R3462 T13099 T13095 compound mr,s
R3463 T13100 T13095 punct -,s
R3464 T13101 T13095 punct (,s
R3465 T13102 T13103 amod full,HA
R3466 T13103 T13095 appos HA,s
R3467 T13104 T13103 punct -,HA
R3468 T13105 T13078 punct ),co-immunoprecipitated
R3469 T13106 T13078 auxpass was,co-immunoprecipitated
R3470 T13107 T13078 prep with,co-immunoprecipitated
R3471 T13108 T13109 npadvmod Flag,tagged
R3472 T13109 T13111 amod tagged,s
R3473 T13110 T13109 punct -,tagged
R3474 T13111 T13107 pobj s,with
R3475 T13112 T13113 amod full,length
R3476 T13113 T13111 compound length,s
R3477 T13114 T13113 punct -,length
R3478 T13115 T13111 compound mr,s
R3479 T13116 T13111 punct -,s
R3480 T13117 T13111 punct (,s
R3481 T13118 T13119 compound Flag,mrs
R3482 T13119 T13111 appos mrs,s
R3483 T13120 T13119 punct -,mrs
R3484 T13121 T13111 punct ),s
R3485 T13122 T13111 cc and,s
R3486 T13123 T13124 det the,portion
R3487 T13124 T13111 conj portion,s
R3488 T13125 T13126 npadvmod Flag,tagged
R3489 T13126 T13124 amod tagged,portion
R3490 T13127 T13126 punct -,tagged
R3491 T13128 T13129 compound C,terminus
R3492 T13129 T13124 compound terminus,portion
R3493 T13130 T13129 punct -,terminus
R3494 T13131 T13124 acl containing,portion
R3495 T13132 T13133 det the,domain
R3496 T13133 T13131 dobj domain,containing
R3497 T13134 T13133 compound SAM,domain
R3498 T13135 T13124 punct (,portion
R3499 T13136 T13137 compound Flag,SAM
R3500 T13137 T13124 appos SAM,portion
R3501 T13138 T13137 punct -,SAM
R3502 T13139 T13078 punct ),co-immunoprecipitated
R3503 T13140 T13078 punct ", ",co-immunoprecipitated
R3504 T13141 T13078 advmod respectively,co-immunoprecipitated
R3505 T13142 T13143 punct (,lane3
R3506 T13143 T13078 parataxis lane3,co-immunoprecipitated
R3507 T13144 T13145 compound Fig,5B
R3508 T13145 T13143 dep 5B,lane3
R3509 T13146 T13143 punct ", ",lane3
R3510 T13147 T13143 cc and,lane3
R3511 T13148 T13143 conj lane5,lane3
R3512 T13149 T13143 punct ),lane3
R3513 T13150 T13078 punct .,co-immunoprecipitated
R3514 T13152 T13153 nsubj We,found
R3515 T13154 T13153 advmod also,found
R3516 T13155 T13156 det a,band
R3517 T13156 T13153 dobj band,found
R3518 T13157 T13156 amod weak,band
R3519 T13158 T13156 compound co-immunoprecipitation,band
R3520 T13159 T13153 prep in,found
R3521 T13160 T13159 pobj co-transfection,in
R3522 T13161 T13160 prep of,co-transfection
R3523 T13162 T13163 amod full,HA
R3524 T13163 T13161 pobj HA,of
R3525 T13164 T13163 punct -,HA
R3526 T13165 T13163 cc and,HA
R3527 T13166 T13167 npadvmod Flag,tagged
R3528 T13167 T13169 amod tagged,portion
R3530 T13168 T13167 punct -,tagged
R3533 T13169 T13163 conj portion,HA
R3536 T13170 T13171 compound N,terminus
R3540 T13171 T13169 compound terminus,portion
R3543 T13172 T13171 punct -,terminus
R3544 T13173 T13169 prep of,portion
R3545 T13174 T13175 compound mr,s
R3546 T13175 T13173 pobj s,of
R3547 T13176 T13175 punct -,s
R3548 T13177 T13169 punct (,portion
R3550 T13178 T13179 compound Flag,ΔSAM
R3552 T13179 T13169 appos ΔSAM,portion
R3555 T13180 T13179 punct -,ΔSAM
R3559 T13181 T13182 punct ", ",lane
R3562 T13182 T13153 parataxis lane,found
R3566 T13183 T13184 compound Fig.,5B
R3570 T13184 T13182 dep 5B,lane
R3573 T13185 T13182 punct ", ",lane
R3577 T13186 T13182 nummod 4,lane
R3581 T13187 T13182 punct ),lane
R3585 T13188 T13153 punct .,found
R3589 T13190 T13191 advmod When,co-transfected
R3590 T13191 T13202 advcl co-transfected,co-immunoprecipitated
R3591 T13277 T13275 pobj s,of
R3592 T13192 T13193 compound ΔSAM,HA
R3593 T13278 T13277 punct -,s
R3594 T13279 T13280 punct (,arrows
R3595 T13280 T13249 parataxis arrows,generated
R3596 T13281 T13282 compound Fig.,1B
R3597 T13282 T13280 dep 1B,arrows
R3598 T13193 T13191 nsubjpass HA,co-transfected
R3599 T13283 T13280 punct ", ",arrows
R3600 T13194 T13193 punct -,HA
R3601 T13195 T13193 cc and,HA
R3602 T13284 T13280 punct ),arrows
R3603 T13196 T13197 npadvmod Flag,tagged
R3604 T13285 T13249 punct .,generated
R3605 T13197 T13199 amod tagged,mutants
R3606 T13287 T13288 det These,mutations
R3607 T13288 T13289 nsubj mutations,alter
R3608 T13198 T13197 punct -,tagged
R3609 T13290 T13289 dobj residues,alter
R3610 T13291 T13292 dep that,conserved
R3611 T13199 T13193 conj mutants,HA
R3612 T13292 T13290 relcl conserved,residues
R3613 T13293 T13292 auxpass are,conserved
R3614 T13294 T13292 prep in,conserved
R3615 T13200 T13199 compound deletion,mutants
R3616 T13295 T13296 det the,domain
R3617 T13296 T13294 pobj domain,in
R3618 T13297 T13296 compound SAM,domain
R3619 T13201 T13191 auxpass were,co-transfected
R3620 T13298 T13296 prep of,domain
R3621 T13299 T13298 pobj ph,of
R3622 T13300 T13289 cc and,alter
R3623 T13203 T13202 punct ", ",co-immunoprecipitated
R3624 T13301 T13302 amod previous,report
R3625 T13302 T13303 nsubj report,indicates
R3626 T13303 T13289 conj indicates,alter
R3627 T13304 T13305 mark that,cause
R3628 T13204 T13205 compound ΔSAM,HA
R3629 T13305 T13303 ccomp cause,indicates
R3630 T13306 T13307 det these,mutations
R3631 T13205 T13202 nsubjpass HA,co-immunoprecipitated
R3632 T13307 T13305 nsubj mutations,cause
R3633 T13308 T13307 prep of,mutations
R3634 T13206 T13205 punct -,HA
R3635 T13309 T13310 compound ph,SAM
R3636 T13310 T13308 pobj SAM,of
R3637 T13311 T13310 punct -,SAM
R3638 T13207 T13202 auxpass was,co-immunoprecipitated
R3639 T13312 T13313 amod significant,reduction
R3640 T13313 T13305 dobj reduction,cause
R3641 T13314 T13313 prep in,reduction
R3642 T13208 T13202 prep with,co-immunoprecipitated
R3643 T13315 T13316 compound binding,activity
R3644 T13316 T13314 pobj activity,in
R3645 T13317 T13316 prep to,activity
R3646 T13209 T13210 compound Flag,mrs
R3647 T13318 T13319 det the,protein
R3648 T13319 T13317 pobj protein,to
R3649 T13320 T13319 amod other,protein
R3650 T13321 T13322 compound SAM,domain
R3651 T13322 T13323 npadvmod domain,containing
R3652 T13210 T13208 pobj mrs,with
R3653 T13323 T13319 amod containing,protein
R3654 T13324 T13323 punct -,containing
R3655 T13325 T13319 punct ", ",protein
R3656 T13326 T13327 compound Sex,comb
R3657 T13211 T13210 punct -,mrs
R3658 T13327 T13319 appos comb,protein
R3659 T13328 T13327 prep on,comb
R3660 T13329 T13328 pobj midleg,on
R3661 T13212 T13210 cc and,mrs
R3662 T13330 T13327 punct (,comb
R3663 T13331 T13327 appos Scm,comb
R3664 T13213 T13214 compound Flag,ΔSAM
R3665 T13332 T13305 punct ),cause
R3666 T13333 T13334 punct (,41
R3667 T13334 T13305 parataxis 41,cause
R3668 T13214 T13210 conj ΔSAM,mrs
R3669 T13335 T13334 punct ),41
R3670 T13336 T13303 punct .,indicates
R3671 T13215 T13214 punct -,ΔSAM
R3672 T13338 T13339 prep Based,introduced
R3673 T13216 T13217 punct (,5B
R3674 T13340 T13338 prep on,Based
R3675 T13341 T13342 det this,result
R3676 T13342 T13340 pobj result,on
R3677 T13217 T13202 parataxis 5B,co-immunoprecipitated
R3678 T13343 T13339 punct ", ",introduced
R3679 T13344 T13339 nsubj we,introduced
R3680 T13345 T13346 nummod two,types
R3681 T13218 T13217 compound Fig.,5B
R3682 T13346 T13339 dobj types,introduced
R3683 T13347 T13346 prep of,types
R3684 T13348 T13349 npadvmod site,directed
R3685 T13219 T13217 punct ", ",5B
R3686 T13349 T13351 amod directed,mutations
R3687 T13350 T13349 punct -,directed
R3688 T13220 T13217 appos lane,5B
R3689 T13351 T13347 pobj mutations,of
R3690 T13352 T13346 punct ", ",types
R3691 T13221 T13220 nummod 8,lane
R3692 T13353 T13354 dep which,correspond
R3693 T13354 T13346 relcl correspond,types
R3694 T13355 T13354 prep to,correspond
R3695 T13222 T13220 cc and,lane
R3696 T13356 T13357 det the,mutations
R3697 T13357 T13355 pobj mutations,to
R3698 T13358 T13357 acl introduced,mutations
R3699 T13223 T13220 conj lane,lane
R3700 T13359 T13358 prep in,introduced
R3701 T13360 T13361 compound ph,protein
R3702 T13361 T13359 pobj protein,in
R3703 T13224 T13223 nummod 9,lane
R3704 T13362 T13358 punct ", ",introduced
R3705 T13225 T13217 punct ),5B
R3706 T13363 T13358 prep into,introduced
R3707 T13364 T13365 npadvmod Flag,tagged
R3708 T13365 T13367 amod tagged,s
R3709 T13226 T13202 punct ", ",co-immunoprecipitated
R3710 T13366 T13365 punct -,tagged
R3711 T13367 T13363 pobj s,into
R3712 T13227 T13228 mark while,co-immunoprecipitated
R3713 T13368 T13369 amod full,length
R3714 T13369 T13367 compound length,s
R3715 T13370 T13369 punct -,length
R3716 T13228 T13202 advcl co-immunoprecipitated,co-immunoprecipitated
R3717 T13371 T13367 compound mr,s
R3718 T13372 T13367 punct -,s
R3719 T13373 T13346 punct (,types
R3720 T13229 T13230 compound ΔSAM,HA
R3721 T13374 T13375 compound Flag,W404A
R3722 T13375 T13346 appos W404A,types
R3723 T13376 T13375 punct -,W404A
R3724 T13230 T13228 nsubjpass HA,co-immunoprecipitated
R3725 T13377 T13375 cc and,W404A
R3726 T13378 T13379 compound Flag,G453A
R3727 T13379 T13375 conj G453A,W404A
R3728 T13231 T13230 punct -,HA
R3729 T13380 T13379 punct -,G453A
R3730 T13381 T13339 punct ),introduced
R3731 T13382 T13339 punct .,introduced
R3732 T13232 T13228 auxpass was,co-immunoprecipitated
R3733 T13384 T13385 nsubj We,found
R3734 T13233 T13228 neg not,co-immunoprecipitated
R3735 T13234 T13228 prep with,co-immunoprecipitated
R3736 T13386 T13387 mark that,reduced
R3737 T13387 T13385 ccomp reduced,found
R3738 T13388 T13389 compound Flag,W404A
R3739 T13235 T13236 compound Flag,SAM
R3740 T13389 T13391 compound W404A,binding
R3741 T13390 T13389 punct -,W404A
R3742 T13391 T13392 compound binding,activity
R3743 T13392 T13387 nsubjpass activity,reduced
R3744 T13393 T13387 auxpass was,reduced
R3745 T13394 T13387 advmod significantly,reduced
R3746 T13395 T13385 cc and,found
R3747 T13396 T13397 compound Flag,G453A
R3748 T13397 T13399 compound G453A,binding
R3749 T13398 T13397 punct -,G453A
R3750 T13399 T13400 compound binding,activity
R3751 T13400 T13401 nsubjpass activity,reduced
R3752 T13401 T13385 conj reduced,found
R3753 T13402 T13401 auxpass was,reduced
R3754 T13403 T13401 advmod also,reduced
R3755 T13404 T13401 advmod slightly,reduced
R3756 T13405 T13401 prep compared,reduced
R3757 T13406 T13405 prep to,compared
R3758 T13407 T13408 compound Flag,mrs
R3759 T13408 T13406 pobj mrs,to
R3760 T13409 T13408 punct -,mrs
R3761 T13410 T13411 punct (,5C
R3762 T13411 T13401 parataxis 5C,reduced
R3763 T13412 T13411 compound Fig.,5C
R3764 T13413 T13411 punct ),5C
R3765 T13414 T13401 punct .,reduced
R3766 T13416 T13417 det These,results
R3767 T13417 T13418 nsubj results,indicate
R3768 T13419 T13418 punct ", ",indicate
R3769 T13420 T13418 advmod together,indicate
R3770 T13421 T13420 prep with,together
R3771 T13422 T13423 nmod yeast,assay
R3772 T13423 T13421 pobj assay,with
R3773 T13424 T13425 nummod two,hybrid
R3774 T13425 T13423 compound hybrid,assay
R3775 T13426 T13425 punct -,hybrid
R3776 T13427 T13423 compound GAL4,assay
R3777 T13428 T13418 punct ", ",indicate
R3778 T13429 T13430 mark that,associates
R3779 T13430 T13418 ccomp associates,indicate
R3780 T13431 T13432 det the,protein
R3781 T13432 T13430 nsubj protein,associates
R3782 T13433 T13434 compound mr,s
R3783 T13434 T13432 compound s,protein
R3784 T13435 T13434 punct -,s
R3785 T13436 T13430 dep self,associates
R3786 T13437 T13430 punct -,associates
R3787 T13438 T13430 advmod strongly,associates
R3788 T13439 T13430 prep through,associates
R3789 T13440 T13441 poss its,domain
R3790 T13441 T13439 pobj domain,through
R3791 T13442 T13441 compound SAM,domain
R3792 T13443 T13430 cc and,associates
R3793 T13444 T13430 conj weakly,associates
R3794 T13445 T13444 prep through,weakly
R3795 T13446 T13447 det the,portion
R3796 T13447 T13445 pobj portion,through
R3797 T13448 T13449 compound N,terminus
R3798 T13449 T13447 compound terminus,portion
R3799 T13450 T13449 punct -,terminus
R3800 T13451 T13447 acl lacking,portion
R3801 T13452 T13453 compound SAM,domain
R3802 T13453 T13451 dobj domain,lacking
R3803 T13454 T13418 punct .,indicate
R3806 T13863 T13864 det The,localization
R3807 T13865 T13864 amod subcellular,localization
R3808 T13866 T13864 prep of,localization
R3809 T13867 T13868 compound mr,s
R3810 T13868 T13870 compound s,protein
R3811 T13869 T13868 punct -,s
R3812 T13870 T13866 pobj protein,of
R3813 T13871 T13864 prep in,localization
R3814 T13872 T13873 amod mammalian,cells
R3815 T13873 T13871 pobj cells,in
R3816 T13875 T13876 det The,signal
R3817 T13876 T13880 nsubj signal,suggests
R3818 T13877 T13876 amod putative,signal
R3819 T13878 T13879 amod nuclear,localization
R3820 T13879 T13876 compound localization,signal
R3821 T13881 T13876 prep at,signal
R3822 T13882 T13883 det the,terminus
R3823 T13883 T13881 pobj terminus,at
R3824 T13884 T13883 compound N,terminus
R3825 T13885 T13883 punct -,terminus
R3826 T13886 T13883 prep of,terminus
R3827 T13887 T13888 det the,protein
R3828 T13888 T13886 pobj protein,of
R3829 T13889 T13890 compound mr,s
R3830 T13890 T13888 compound s,protein
R3831 T13891 T13890 punct -,s
R3832 T13892 T13893 punct (,box
R3833 T13893 T13876 parataxis box,signal
R3834 T13894 T13895 compound Fig.,1A
R3835 T13895 T13893 dep 1A,box
R3836 T13896 T13893 punct ", ",box
R3837 T13897 T13893 amod dashed,box
R3838 T13898 T13893 punct ),box
R3839 T13899 T13900 mark that,localizes
R3840 T13900 T13880 ccomp localizes,suggests
R3841 T13901 T13902 det the,protein
R3842 T13902 T13900 nsubj protein,localizes
R3843 T13903 T13904 compound mr,s
R3844 T13904 T13902 compound s,protein
R3845 T13905 T13904 punct -,s
R3846 T13906 T13900 prep in,localizes
R3847 T13907 T13908 det the,nucleus
R3848 T13908 T13906 pobj nucleus,in
R3849 T13909 T13880 punct .,suggests
R3850 T13911 T13912 aux To,determine
R3851 T13912 T13913 advcl determine,introduced
R3852 T13914 T13915 det the,localization
R3853 T13915 T13912 dobj localization,determine
R3854 T13916 T13915 amod subcellular,localization
R3855 T13917 T13915 prep of,localization
R3856 T13918 T13919 compound mr,s
R3857 T13919 T13917 pobj s,of
R3858 T13920 T13919 punct -,s
R3859 T13921 T13912 prep in,determine
R3860 T13922 T13923 amod mammalian,cells
R3861 T13923 T13921 pobj cells,in
R3862 T13924 T13913 punct ", ",introduced
R3863 T13925 T13913 nsubj we,introduced
R3864 T13926 T13927 det an,plasmid
R3865 T13927 T13913 dobj plasmid,introduced
R3866 T13928 T13929 npadvmod HA,tagged
R3867 T13929 T13927 amod tagged,plasmid
R3868 T13930 T13929 punct -,tagged
R3869 T13931 T13932 amod full,length
R3870 T13932 T13927 compound length,plasmid
R3871 T13933 T13932 punct -,length
R3872 T13934 T13935 compound mr,s
R3873 T13935 T13927 compound s,plasmid
R3874 T13936 T13935 punct -,s
R3875 T13937 T13913 prep into,introduced
R3876 T13938 T13939 compound HEK293T,cells
R3877 T13939 T13937 pobj cells,into
R3878 T13940 T13913 punct .,introduced
R3879 T13942 T13943 amod Confocal,microscopy
R3880 T13943 T13944 nsubj microscopy,showed
R3881 T13945 T13946 mark that,localized
R3882 T13946 T13944 ccomp localized,showed
R3883 T13947 T13948 det the,protein
R3884 T13948 T13946 nsubj protein,localized
R3885 T13949 T13950 compound mr,s
R3886 T13950 T13948 compound s,protein
R3887 T13951 T13950 punct -,s
R3888 T13952 T13953 advmod mainly,in
R3889 T13953 T13946 prep in,localized
R3890 T13954 T13955 det the,nucleus
R3891 T13955 T13953 pobj nucleus,in
R3892 T13956 T13955 prep of,nucleus
R3893 T13957 T13958 compound HEK293T,cells
R3894 T13958 T13956 pobj cells,of
R3895 T13959 T13960 punct (,B
R3896 T13960 T13944 parataxis B,showed
R3897 T13961 T13960 nmod Fig.,B
R3898 T13962 T13960 nmod 6A,B
R3899 T13963 T13960 punct ", ",B
R3900 T13964 T13960 punct ),B
R3901 T13965 T13944 punct .,showed
R3902 T13967 T13968 aux To,confirm
R3903 T13968 T13969 advcl confirm,co-immunostained
R3904 T13970 T13971 det the,localization
R3905 T13971 T13968 dobj localization,confirm
R3906 T13972 T13971 amod precise,localization
R3907 T13973 T13971 prep of,localization
R3908 T13974 T13975 det the,protein
R3909 T13975 T13973 pobj protein,of
R3910 T13976 T13977 compound mr,s
R3911 T13977 T13975 compound s,protein
R3912 T13978 T13977 punct -,s
R3913 T13979 T13969 punct ", ",co-immunostained
R3914 T13980 T13981 amod full,length
R3915 T13981 T13983 compound length,s
R3916 T13982 T13981 punct -,length
R3917 T13983 T13969 nsubjpass s,co-immunostained
R3918 T13984 T13983 compound mr,s
R3919 T13985 T13983 punct -,s
R3920 T13986 T13969 auxpass was,co-immunostained
R3921 T13987 T13969 prep with,co-immunostained
R3922 T13988 T13987 pobj DAPI,with
R3923 T13989 T13990 punct (,6C
R3924 T13990 T13969 parataxis 6C,co-immunostained
R3925 T13991 T13990 compound Fig.,6C
R3926 T13992 T13993 punct –,E
R3927 T13993 T13990 prep E,6C
R3928 T13994 T13990 punct ),6C
R3929 T13995 T13969 punct .,co-immunostained
R3930 T13997 T13998 det These,data
R3931 T13998 T13999 nsubj data,showed
R3932 T14000 T14001 mark that,localizes
R3933 T14001 T13999 ccomp localizes,showed
R3934 T14002 T14003 det the,protein
R3935 T14003 T14001 nsubj protein,localizes
R3936 T14004 T14005 compound mr,s
R3937 T14005 T14003 compound s,protein
R3938 T14006 T14005 punct -,s
R3939 T14007 T14001 advmod preferentially,localizes
R3940 T14008 T14001 prep to,localizes
R3941 T14009 T14010 det the,nucleus
R3942 T14010 T14008 pobj nucleus,to
R3943 T14011 T14001 prep in,localizes
R3944 T14012 T14013 amod mammalian,cells
R3945 T14013 T14011 pobj cells,in
R3946 T14014 T14001 punct ", ",localizes
R3947 T14015 T14001 advcl supporting,localizes
R3948 T14016 T14017 det the,idea
R3949 T14017 T14015 dobj idea,supporting
R3950 T14018 T14019 mark that,involved
R3951 T14019 T14017 acl involved,idea
R3952 T14020 T14021 det the,protein
R3953 T14021 T14019 nsubjpass protein,involved
R3954 T14022 T14023 compound mr,s
R3955 T14023 T14021 compound s,protein
R3956 T14024 T14023 punct -,s
R3957 T14025 T14019 auxpass is,involved
R3958 T14026 T14019 prep in,involved
R3959 T14027 T14028 amod transcriptional,regulation
R3960 T14028 T14026 pobj regulation,in
R3961 T14029 T14030 mark as,are
R3962 T14030 T14019 advcl are,involved
R3963 T14031 T14030 nsubj ph,are
R3964 T14032 T14031 cc and,ph
R3965 T14033 T14032 punct /,and
R3966 T14034 T14032 cc or,and
R3967 T14035 T14031 conj TEL,ph
R3968 T14036 T13999 punct .,showed
R3969 T16058 T16059 det The,protein
R3970 T16059 T16066 nsubj protein,functions
R3971 T16060 T16061 nmod GAL4,fusion
R3972 T16061 T16059 compound fusion,protein
R3973 T16062 T16060 punct -,GAL4
R3974 T16063 T16064 compound mr,s
R3975 T16064 T16060 appos s,GAL4
R3976 T16065 T16064 punct -,s
R3977 T16067 T16066 prep as,functions
R3978 T16068 T16069 amod transcriptional,repressor
R3979 T16069 T16067 pobj repressor,as
R3980 T16071 T16072 det A,member
R3981 T16072 T16073 nsubj member,contain
R3982 T16074 T16072 prep of,member
R3983 T16075 T16076 compound PcG,proteins
R3984 T16076 T16074 pobj proteins,of
R3985 T16077 T16072 punct ", ",member
R3986 T16078 T16072 appos ph,member
R3987 T16079 T16073 punct ", ",contain
R3988 T16080 T16073 aux does,contain
R3989 T16081 T16073 neg not,contain
R3990 T16082 T16083 det an,motif
R3991 T16083 T16073 dobj motif,contain
R3992 T16084 T16083 amod obvious,motif
R3993 T16085 T16086 npadvmod sequence,specific
R3994 T16086 T16083 amod specific,motif
R3995 T16087 T16086 punct -,specific
R3996 T16088 T16089 npadvmod DNA,binding
R3997 T16089 T16083 amod binding,motif
R3998 T16090 T16091 punct (,16
R3999 T16091 T16073 parataxis 16,contain
R4000 T16092 T16091 punct ),16
R4001 T16093 T16073 punct .,contain
R4002 T16095 T16096 nsubj Ph,functions
R4003 T16097 T16096 prep as,functions
R4004 T16098 T16099 det a,repressor
R4005 T16099 T16097 pobj repressor,as
R4006 T16100 T16099 amod transcriptional,repressor
R4007 T16101 T16096 prep through,functions
R4008 T16102 T16103 poss its,polymerization
R4009 T16103 T16101 pobj polymerization,through
R4010 T16104 T16103 cc and,polymerization
R4011 T16105 T16106 compound protein,protein
R4012 T16106 T16108 compound protein,interaction
R4013 T16107 T16106 punct -,protein
R4014 T16108 T16103 conj interaction,polymerization
R4015 T16109 T16108 prep with,interaction
R4016 T16110 T16111 amod other,repressors
R4017 T16111 T16109 pobj repressors,with
R4018 T16112 T16113 npadvmod sequence,specific
R4019 T16113 T16111 amod specific,repressors
R4020 T16114 T16113 punct -,specific
R4021 T16115 T16111 amod transcriptional,repressors
R4022 T16116 T16111 punct ", ",repressors
R4023 T16117 T16118 dep which,form
R4024 T16118 T16111 relcl form,repressors
R4025 T16119 T16118 aux can,form
R4026 T16120 T16121 det a,structure
R4027 T16121 T16118 dobj structure,form
R4028 T16122 T16123 amod higher,order
R4029 T16123 T16121 compound order,structure
R4030 T16124 T16121 compound chromatin,structure
R4031 T16125 T16096 punct .,functions
R4032 T16127 T16128 det The,protein
R4033 T16128 T16132 nsubj protein,have
R4034 T16129 T16130 compound mr,s
R4035 T16130 T16128 compound s,protein
R4036 T16131 T16130 punct -,s
R4037 T16133 T16132 advmod also,have
R4038 T16134 T16132 aux does,have
R4039 T16135 T16132 neg not,have
R4040 T16136 T16137 det an,domain
R4041 T16137 T16132 dobj domain,have
R4042 T16138 T16137 amod obvious,domain
R4043 T16139 T16140 npadvmod DNA,binding
R4044 T16140 T16137 amod binding,domain
R4045 T16141 T16140 punct -,binding
R4046 T16142 T16132 punct .,have
R4047 T16144 T16145 aux To,characterize
R4048 T16145 T16146 advcl characterize,performed
R4049 T16147 T16148 det the,activity
R4050 T16148 T16145 dobj activity,characterize
R4051 T16149 T16148 amod biochemical,activity
R4052 T16150 T16148 prep of,activity
R4053 T16151 T16152 compound mr,s
R4054 T16152 T16150 pobj s,of
R4055 T16153 T16152 punct -,s
R4056 T16154 T16146 punct ", ",performed
R4057 T16155 T16146 nsubj we,performed
R4058 T16156 T16146 advmod next,performed
R4059 T16157 T16158 det a,assay
R4060 T16158 T16146 dobj assay,performed
R4061 T16159 T16158 compound luciferase,assay
R4062 T16160 T16146 punct .,performed
R4063 T16162 T16163 nsubj We,generated
R4064 T16164 T16165 compound effector,plasmids
R4065 T16165 T16163 dobj plasmids,generated
R4066 T16166 T16165 punct ", ",plasmids
R4067 T16167 T16168 dep which,express
R4068 T16168 T16165 relcl express,plasmids
R4069 T16169 T16170 amod various,constructs
R4070 T16170 T16168 dobj constructs,express
R4071 T16171 T16170 compound deletion,constructs
R4072 T16172 T16170 prep of,constructs
R4073 T16173 T16174 compound mr,s
R4074 T16174 T16172 pobj s,of
R4075 T16175 T16174 punct -,s
R4076 T16176 T16170 acl fused,constructs
R4077 T16177 T16176 prep to,fused
R4078 T16178 T16179 det the,domain
R4079 T16179 T16177 pobj domain,to
R4080 T16180 T16179 nmod GAL4,domain
R4081 T16181 T16182 npadvmod DNA,binding
R4082 T16182 T16179 amod binding,domain
R4083 T16183 T16182 punct -,binding
R4084 T16184 T16185 punct (,7A
R4085 T16185 T16163 parataxis 7A,generated
R4086 T16186 T16185 compound Fig.,7A
R4087 T16187 T16185 punct ),7A
R4088 T16188 T16163 punct .,generated
R4089 T16190 T16191 nsubj We,confirmed
R4090 T16192 T16191 advmod first,confirmed
R4091 T16193 T16194 mark that,had
R4092 T16194 T16191 ccomp had,confirmed
R4093 T16195 T16196 amod full,length
R4094 T16196 T16198 compound length,s
R4095 T16197 T16196 punct -,length
R4096 T16198 T16194 nsubj s,had
R4097 T16199 T16198 compound mr,s
R4098 T16200 T16198 punct -,s
R4099 T16201 T16198 acl fused,s
R4100 T16202 T16201 prep to,fused
R4101 T16203 T16204 compound GAL4,domain
R4102 T16204 T16202 pobj domain,to
R4103 T16205 T16206 compound DNA,binding
R4104 T16206 T16204 compound binding,domain
R4105 T16207 T16204 punct (,domain
R4106 T16208 T16209 compound DBD,mrs
R4107 T16209 T16204 appos mrs,domain
R4108 T16210 T16209 punct -,mrs
R4109 T16211 T16194 punct ),had
R4110 T16212 T16213 det no,effect
R4111 T16213 T16194 dobj effect,had
R4112 T16214 T16213 prep on,effect
R4113 T16215 T16216 det the,plasmid
R4114 T16216 T16214 pobj plasmid,on
R4115 T16217 T16216 compound pGL3,plasmid
R4116 T16218 T16216 compound promoter,plasmid
R4117 T16219 T16216 acl lacking,plasmid
R4118 T16220 T16221 compound GAL4,binding
R4119 T16221 T16222 compound binding,sites
R4120 T16222 T16219 dobj sites,lacking
R4121 T16223 T16224 punct (,shown
R4122 T16224 T16191 parataxis shown,confirmed
R4123 T16225 T16224 nsubj data,shown
R4124 T16226 T16224 neg not,shown
R4125 T16227 T16224 punct ),shown
R4126 T16228 T16191 punct .,confirmed
R4127 T16230 T16231 advmod When,co-transfected
R4128 T16231 T16239 advcl co-transfected,repressed
R4129 T16232 T16233 det the,plasmid
R4130 T16233 T16231 nsubjpass plasmid,co-transfected
R4131 T16234 T16235 compound 5xGAL4,pGL3
R4132 T16235 T16233 compound pGL3,plasmid
R4133 T16236 T16235 punct -,pGL3
R4134 T16237 T16233 compound reporter,plasmid
R4135 T16238 T16231 auxpass was,co-transfected
R4136 T16240 T16239 punct ", ",repressed
R4137 T16241 T16242 compound DBD,mrs
R4138 T16242 T16239 nsubj mrs,repressed
R4139 T16243 T16242 punct -,mrs
R4140 T16244 T16245 compound luciferase,activity
R4141 T16245 T16239 dobj activity,repressed
R4142 T16246 T16239 prep by,repressed
R4143 T16247 T16248 quantmod about,90
R4144 T16248 T16249 nummod 90,%
R4145 T16249 T16246 pobj %,by
R4146 T16250 T16239 prep in,repressed
R4147 T16251 T16252 det a,manner
R4148 T16252 T16250 pobj manner,in
R4149 T16253 T16254 npadvmod dose,dependent
R4150 T16254 T16252 amod dependent,manner
R4151 T16255 T16254 punct -,dependent
R4152 T16256 T16257 punct (,7B
R4153 T16257 T16239 parataxis 7B,repressed
R4154 T16258 T16257 compound Fig.,7B
R4155 T16259 T16257 punct ),7B
R4156 T16260 T16239 punct .,repressed
R4157 T16262 T16263 prep As,had
R4158 T16264 T16265 det a,control
R4159 T16265 T16262 pobj control,As
R4160 T16266 T16263 punct ", ",had
R4161 T16267 T16268 nmod GAL4,domain
R4162 T16268 T16263 nsubj domain,had
R4163 T16269 T16270 npadvmod DNA,binding
R4164 T16270 T16268 amod binding,domain
R4165 T16271 T16270 punct -,binding
R4166 T16272 T16268 punct (,domain
R4167 T16273 T16268 appos DBD,domain
R4168 T16274 T16263 punct ),had
R4169 T16275 T16276 det no,effect
R4170 T16276 T16263 dobj effect,had
R4171 T16277 T16276 amod significant,effect
R4172 T16278 T16276 prep on,effect
R4173 T16279 T16280 det the,plasmid
R4174 T16280 T16278 pobj plasmid,on
R4175 T16281 T16282 compound 5xGAL4,pGL3
R4176 T16282 T16280 compound pGL3,plasmid
R4177 T16283 T16282 punct -,pGL3
R4178 T16284 T16280 compound reporter,plasmid
R4179 T16285 T16263 punct .,had
R4180 T16287 T16288 prep In,confirmed
R4181 T16289 T16287 pobj addition,In
R4182 T16290 T16288 punct ", ",confirmed
R4183 T16291 T16288 nsubj we,confirmed
R4184 T16292 T16293 mark that,had
R4185 T16293 T16288 ccomp had,confirmed
R4186 T16294 T16295 amod full,length
R4187 T16295 T16297 compound length,s
R4188 T16296 T16295 punct -,length
R4189 T16297 T16293 nsubj s,had
R4190 T16298 T16297 compound mr,s
R4191 T16299 T16297 punct -,s
R4192 T16300 T16297 prep without,s
R4193 T16301 T16302 compound GAL4,DBD
R4194 T16302 T16300 pobj DBD,without
R4195 T16303 T16304 det no,effect
R4196 T16304 T16293 dobj effect,had
R4197 T16305 T16304 prep on,effect
R4198 T16306 T16307 det the,plasmid
R4199 T16307 T16305 pobj plasmid,on
R4200 T16308 T16307 amod same,plasmid
R4201 T16309 T16307 compound reporter,plasmid
R4202 T16310 T16311 punct (,shown
R4203 T16311 T16288 parataxis shown,confirmed
R4204 T16312 T16311 nsubj data,shown
R4205 T16313 T16311 neg not,shown
R4206 T16314 T16311 punct ),shown
R4207 T16315 T16288 punct .,confirmed
R4208 T16317 T16318 nsubj We,analyzed
R4209 T16319 T16318 advmod next,analyzed
R4210 T16320 T16321 compound deletion,constructs
R4211 T16321 T16318 dobj constructs,analyzed
R4212 T16322 T16323 prep in,fused
R4213 T16323 T16321 relcl fused,constructs
R4214 T16324 T16322 pobj which,in
R4215 T16325 T16326 det the,stretch
R4216 T16326 T16323 nsubjpass stretch,fused
R4217 T16327 T16328 nmod N,terminus
R4218 T16328 T16326 nmod terminus,stretch
R4219 T16329 T16328 punct -,terminus
R4220 T16330 T16331 nummod 400,aa
R4221 T16331 T16326 compound aa,stretch
R4222 T16332 T16326 prep of,stretch
R4223 T16333 T16334 compound mr,s
R4224 T16334 T16332 pobj s,of
R4225 T16335 T16334 punct -,s
R4226 T16336 T16337 punct (,1
R4227 T16337 T16326 parataxis 1,stretch
R4228 T16338 T16337 nmod amino,1
R4229 T16339 T16337 nmod acids,1
R4230 T16340 T16337 prep to,1
R4231 T16341 T16340 pobj 400,to
R4232 T16342 T16337 punct ),1
R4233 T16343 T16326 cc or,stretch
R4234 T16344 T16345 det the,portion
R4235 T16345 T16326 conj portion,stretch
R4236 T16346 T16347 compound C,terminus
R4237 T16347 T16345 compound terminus,portion
R4238 T16348 T16347 punct -,terminus
R4239 T16349 T16350 punct (,391
R4240 T16350 T16345 parataxis 391,portion
R4241 T16351 T16350 nmod amino,391
R4242 T16352 T16350 nmod acids,391
R4243 T16353 T16350 prep to,391
R4244 T16354 T16353 pobj 542,to
R4245 T16355 T16350 punct ),391
R4246 T16356 T16323 auxpass were,fused
R4247 T16357 T16323 prep to,fused
R4248 T16358 T16359 det the,DBD
R4249 T16359 T16357 pobj DBD,to
R4250 T16360 T16359 compound GAL4,DBD
R4251 T16361 T16362 punct (,7A
R4252 T16362 T16323 parataxis 7A,fused
R4253 T16363 T16362 compound Fig.,7A
R4254 T16364 T16362 punct ", ",7A
R4255 T16365 T16366 compound DBD,N
R4256 T16366 T16362 appos N,7A
R4257 T16367 T16366 punct -,N
R4258 T16368 T16362 punct ", ",7A
R4259 T16369 T16370 compound DBD,C
R4260 T16370 T16362 appos C,7A
R4261 T16371 T16370 punct -,C
R4262 T16372 T16362 punct ),7A
R4263 T16373 T16318 punct .,analyzed
R4264 T16375 T16376 mark While,had
R4265 T16376 T16380 advcl had,repressed
R4266 T16377 T16378 compound DBD,N
R4267 T16378 T16376 nsubj N,had
R4268 T16379 T16378 punct -,N
R4269 T16381 T16382 det no,effect
R4270 T16382 T16376 dobj effect,had
R4271 T16383 T16382 amod repressive,effect
R4272 T16384 T16382 prep on,effect
R4273 T16385 T16386 det this,activity
R4274 T16386 T16384 pobj activity,on
R4275 T16387 T16386 compound reporter,activity
R4276 T16388 T16380 punct ", ",repressed
R4277 T16389 T16390 compound DBD,C
R4278 T16390 T16380 nsubj C,repressed
R4279 T16391 T16390 punct -,C
R4280 T16392 T16393 compound luciferase,expression
R4281 T16393 T16380 dobj expression,repressed
R4282 T16394 T16393 compound gene,expression
R4283 T16395 T16380 prep by,repressed
R4284 T16396 T16397 quantmod about,65
R4285 T16397 T16398 nummod 65,%
R4286 T16398 T16395 pobj %,by
R4287 T16399 T16380 punct .,repressed
R4288 T16401 T16402 det This,result
R4289 T16402 T16403 nsubj result,suggested
R4290 T16404 T16403 prep to,suggested
R4291 T16405 T16404 pobj us,to
R4292 T16406 T16407 det the,possibility
R4293 T16407 T16403 dobj possibility,suggested
R4294 T16408 T16409 mark that,exerts
R4295 T16409 T16407 acl exerts,possibility
R4296 T16410 T16411 compound DBD,mrs
R4297 T16411 T16409 nsubj mrs,exerts
R4298 T16412 T16411 punct -,mrs
R4299 T16413 T16414 amod transcriptional,activity
R4300 T16414 T16409 dobj activity,exerts
R4301 T16415 T16414 amod repressive,activity
R4302 T16416 T16409 prep via,exerts
R4303 T16417 T16418 compound self,association
R4304 T16418 T16416 pobj association,via
R4305 T16419 T16418 punct -,association
R4306 T16420 T16409 prep through,exerts
R4307 T16421 T16422 poss its,domain
R4308 T16422 T16420 pobj domain,through
R4309 T16423 T16422 compound SAM,domain
R4310 T16424 T16403 punct .,suggested
R4311 T16426 T16427 aux To,investigate
R4312 T16427 T16428 advcl investigate,analyzed
R4313 T16429 T16430 mark whether,required
R4314 T16430 T16427 ccomp required,investigate
R4315 T16431 T16432 det the,association
R4316 T16432 T16430 nsubjpass association,required
R4317 T16433 T16432 amod homophilic,association
R4318 T16434 T16432 prep of,association
R4319 T16435 T16436 compound mr,s
R4320 T16436 T16434 pobj s,of
R4321 T16437 T16436 punct -,s
R4322 T16438 T16430 auxpass is,required
R4323 T16439 T16430 prep for,required
R4324 T16440 T16441 amod transcriptional,repression
R4325 T16441 T16439 pobj repression,for
R4326 T16442 T16428 punct ", ",analyzed
R4327 T16443 T16444 nummod two,mutants
R4328 T16444 T16428 nsubjpass mutants,analyzed
R4329 T16445 T16446 npadvmod site,directed
R4330 T16446 T16444 amod directed,mutants
R4331 T16447 T16446 punct -,directed
R4332 T16448 T16444 punct ", ",mutants
R4333 T16449 T16450 compound DBD,W404A
R4334 T16450 T16444 appos W404A,mutants
R4335 T16451 T16450 punct -,W404A
R4336 T16452 T16450 cc and,W404A
R4337 T16453 T16454 compound DBD,G453A
R4338 T16454 T16450 conj G453A,W404A
R4339 T16455 T16454 punct -,G453A
R4340 T16456 T16444 punct ", ",mutants
R4341 T16457 T16458 dep either,reduce
R4342 T16458 T16444 relcl reduce,mutants
R4343 T16459 T16457 prep of,either
R4344 T16460 T16459 pobj which,of
R4345 T16461 T16458 aux may,reduce
R4346 T16462 T16463 npadvmod self,binding
R4347 T16463 T16465 amod binding,ability
R4348 T16464 T16463 punct -,binding
R4349 T16465 T16458 dobj ability,reduce
R4350 T16466 T16428 punct ", ",analyzed
R4351 T16467 T16428 auxpass were,analyzed
R4352 T16468 T16469 punct (,7C
R4353 T16469 T16428 parataxis 7C,analyzed
R4354 T16470 T16469 compound Fig.,7C
R4355 T16471 T16469 punct ),7C
R4356 T16472 T16428 punct .,analyzed
R4357 T16474 T16475 prep Compared,had
R4358 T16476 T16474 prep to,Compared
R4359 T16477 T16478 compound DBD,mrs
R4360 T16478 T16476 pobj mrs,to
R4361 T16479 T16478 punct -,mrs
R4362 T16480 T16475 punct ", ",had
R4363 T16481 T16482 compound DBD,W404A
R4364 T16482 T16475 nsubj W404A,had
R4365 T16483 T16482 punct -,W404A
R4366 T16484 T16482 cc and,W404A
R4367 T16485 T16486 compound DBD,G453A
R4368 T16486 T16482 conj G453A,W404A
R4369 T16487 T16486 punct -,G453A
R4370 T16488 T16489 compound repression,activity
R4371 T16489 T16475 dobj activity,had
R4372 T16490 T16489 prep of,activity
R4373 T16491 T16492 nummod 72,%
R4374 T16492 T16490 pobj %,of
R4375 T16493 T16492 cc and,%
R4376 T16494 T16495 nummod 87,%
R4377 T16495 T16492 conj %,%
R4378 T16496 T16475 punct ", ",had
R4379 T16497 T16475 advmod respectively,had
R4380 T16498 T16475 punct .,had
R4381 T16500 T16501 mark While,correlates
R4382 T16501 T16512 advcl correlates,compromise
R4383 T16502 T16503 det the,ability
R4384 T16503 T16501 nsubj ability,correlates
R4385 T16504 T16503 prep of,ability
R4386 T16505 T16506 compound mr,s
R4387 T16506 T16508 compound s,association
R4388 T16507 T16506 punct -,s
R4389 T16508 T16504 pobj association,of
R4390 T16509 T16508 compound self,association
R4391 T16510 T16508 punct -,association
R4392 T16511 T16501 advmod partially,correlates
R4393 T16513 T16501 prep with,correlates
R4394 T16514 T16515 amod transcriptional,activity
R4395 T16515 T16513 pobj activity,with
R4396 T16516 T16515 amod repressive,activity
R4397 T16517 T16512 punct ", ",compromise
R4398 T16518 T16519 det these,mutants
R4399 T16519 T16512 nsubj mutants,compromise
R4400 T16520 T16512 aux do,compromise
R4401 T16521 T16512 neg not,compromise
R4402 T16522 T16523 det the,ability
R4403 T16523 T16512 dobj ability,compromise
R4404 T16524 T16525 aux to,repress
R4405 T16525 T16523 acl repress,ability
R4406 T16526 T16525 dobj transcription,repress
R4407 T16527 T16525 advmod critically,repress
R4408 T16528 T16512 punct .,compromise
R4409 T16530 T16531 aux To,determine
R4410 T16531 T16532 advcl determine,divided
R4411 T16533 T16534 det the,regions
R4412 T16534 T16531 dobj regions,determine
R4413 T16535 T16534 prep of,regions
R4414 T16536 T16537 compound mr,s
R4415 T16537 T16535 pobj s,of
R4416 T16538 T16537 punct -,s
R4417 T16539 T16534 acl involved,regions
R4418 T16540 T16539 prep in,involved
R4419 T16541 T16542 amod transcriptional,repression
R4420 T16542 T16540 pobj repression,in
R4421 T16543 T16544 advmod more,precisely
R4422 T16544 T16531 advmod precisely,determine
R4423 T16545 T16532 punct ", ",divided
R4424 T16546 T16547 det the,portion
R4425 T16547 T16532 nsubjpass portion,divided
R4426 T16548 T16549 compound C,terminus
R4427 T16549 T16547 compound terminus,portion
R4428 T16550 T16549 punct -,terminus
R4429 T16551 T16532 auxpass was,divided
R4430 T16552 T16532 prep into,divided
R4431 T16553 T16554 nummod two,regions
R4432 T16554 T16552 pobj regions,into
R4433 T16555 T16532 cc and,divided
R4434 T16556 T16557 nsubjpass each,fused
R4435 T16557 T16532 conj fused,divided
R4436 T16558 T16557 auxpass was,fused
R4437 T16559 T16557 prep to,fused
R4438 T16560 T16561 det the,DBD
R4439 T16561 T16559 pobj DBD,to
R4440 T16562 T16561 compound GAL4,DBD
R4441 T16563 T16564 punct (,SAM
R4442 T16564 T16557 parataxis SAM,fused
R4443 T16565 T16566 compound Fig.,7A
R4444 T16566 T16564 dep 7A,SAM
R4445 T16567 T16564 punct ", ",SAM
R4446 T16568 T16569 compound DBD,tail
R4447 T16569 T16564 dep tail,SAM
R4448 T16570 T16569 punct -,tail
R4449 T16571 T16564 punct ", ",SAM
R4450 T16572 T16564 compound DBD,SAM
R4451 T16573 T16564 punct -,SAM
R4452 T16574 T16564 punct ),SAM
R4453 T16575 T16532 punct .,divided
R4454 T16577 T16578 prep As,have
R4455 T16579 T16580 det a,consequence
R4456 T16580 T16577 pobj consequence,As
R4457 T16581 T16578 punct ", ",have
R4458 T16582 T16583 compound DBD,SAM
R4459 T16583 T16578 nsubj SAM,have
R4460 T16584 T16583 punct -,SAM
R4461 T16585 T16586 punct (,384
R4462 T16586 T16583 parataxis 384,SAM
R4463 T16587 T16586 nmod amino,384
R4464 T16588 T16586 nmod acids,384
R4465 T16589 T16586 prep to,384
R4466 T16590 T16589 pobj 462,to
R4467 T16591 T16586 punct ),384
R4468 T16592 T16578 aux did,have
R4469 T16593 T16578 neg not,have
R4470 T16594 T16595 det a,effect
R4471 T16595 T16578 dobj effect,have
R4472 T16596 T16595 amod repressive,effect
R4473 T16597 T16595 prep on,effect
R4474 T16598 T16599 det the,plasmid
R4475 T16599 T16597 pobj plasmid,on
R4476 T16600 T16601 compound 5xGAL4,pGL3
R4477 T16601 T16599 compound pGL3,plasmid
R4478 T16602 T16601 punct -,pGL3
R4479 T16603 T16599 compound reporter,plasmid
R4480 T16604 T16578 punct .,have
R4481 T16606 T16607 prep On,repressed
R4482 T16608 T16609 det the,hand
R4483 T16609 T16606 pobj hand,On
R4484 T16610 T16609 amod other,hand
R4485 T16611 T16607 punct ", ",repressed
R4486 T16612 T16613 compound luciferase,activity
R4487 T16613 T16607 nsubjpass activity,repressed
R4488 T16614 T16607 auxpass was,repressed
R4489 T16615 T16607 prep by,repressed
R4490 T16616 T16617 nummod 55,%
R4491 T16617 T16615 pobj %,by
R4492 T16618 T16619 advmod when,co-transfected
R4493 T16619 T16607 advcl co-transfected,repressed
R4494 T16620 T16621 compound DBD,tail
R4495 T16621 T16619 nsubjpass tail,co-transfected
R4496 T16622 T16621 punct -,tail
R4497 T16623 T16624 punct (,459
R4498 T16624 T16621 parataxis 459,tail
R4499 T16625 T16624 nmod amino,459
R4500 T16626 T16624 nmod acids,459
R4501 T16627 T16624 prep to,459
R4502 T16628 T16627 pobj 542,to
R4503 T16629 T16624 punct ),459
R4504 T16630 T16619 auxpass was,co-transfected
R4505 T16631 T16619 prep with,co-transfected
R4506 T16632 T16633 det this,plasmid
R4507 T16633 T16631 pobj plasmid,with
R4508 T16634 T16633 compound reporter,plasmid
R4509 T16635 T16636 punct (,7D
R4510 T16636 T16619 parataxis 7D,co-transfected
R4511 T16637 T16636 compound Fig.,7D
R4512 T16638 T16636 punct ),7D
R4513 T16639 T16607 punct .,repressed
R4514 T16641 T16642 aux To,assess
R4515 T16642 T16643 advcl assess,performed
R4516 T16644 T16645 det the,activity
R4517 T16645 T16642 dobj activity,assess
R4518 T16646 T16645 amod transcriptional,activity
R4519 T16647 T16645 amod repressive,activity
R4520 T16648 T16645 prep of,activity
R4521 T16649 T16650 compound mr,s
R4522 T16650 T16648 pobj s,of
R4523 T16651 T16650 punct -,s
R4524 T16652 T16642 prep in,assess
R4525 T16653 T16652 pobj cells,in
R4526 T16654 T16653 prep of,cells
R4527 T16655 T16656 amod retinal,origin
R4528 T16656 T16654 pobj origin,of
R4529 T16657 T16643 punct ", ",performed
R4530 T16658 T16643 nsubj we,performed
R4531 T16659 T16660 amod similar,experiments
R4532 T16660 T16643 dobj experiments,performed
R4533 T16661 T16643 advcl using,performed
R4534 T16662 T16663 amod human,cells
R4535 T16663 T16661 dobj cells,using
R4536 T16664 T16663 compound Y79,cells
R4537 T16665 T16663 compound retinoblastoma,cells
R4538 T16666 T16643 punct .,performed
R4539 T16668 T16669 det The,results
R4540 T16669 T16670 nsubj results,indicated
R4541 T16671 T16672 mark that,reduced
R4542 T16672 T16670 ccomp reduced,indicated
R4543 T16673 T16674 compound DBD,mrs
R4544 T16674 T16672 nsubj mrs,reduced
R4545 T16675 T16674 punct -,mrs
R4546 T16676 T16672 advmod also,reduced
R4547 T16677 T16678 compound luciferase,activity
R4548 T16678 T16672 dobj activity,reduced
R4549 T16679 T16672 advmod significantly,reduced
R4550 T16680 T16672 prep in,reduced
R4551 T16681 T16682 compound Y79,cells
R4552 T16682 T16680 pobj cells,in
R4553 T16683 T16682 compound retinoblastoma,cells
R4554 T16684 T16685 punct (,7E
R4555 T16685 T16672 parataxis 7E,reduced
R4556 T16686 T16685 compound Fig.,7E
R4557 T16687 T16685 punct ),7E
R4558 T16688 T16670 punct .,indicated
R4559 T16690 T16691 advmod However,repressed
R4560 T16692 T16691 punct ", ",repressed
R4561 T16693 T16694 compound luciferase,activity
R4562 T16694 T16691 nsubjpass activity,repressed
R4563 T16695 T16691 auxpass was,repressed
R4564 T16696 T16691 prep by,repressed
R4565 T16697 T16698 quantmod about,30
R4566 T16698 T16699 nummod 30,%
R4567 T16699 T16696 pobj %,by
R4568 T16700 T16691 prep in,repressed
R4569 T16701 T16702 compound Y79,cells
R4570 T16702 T16700 pobj cells,in
R4571 T16703 T16691 punct ", ",repressed
R4572 T16704 T16705 mark while,repressed
R4573 T16705 T16691 advcl repressed,repressed
R4574 T16706 T16705 nsubjpass it,repressed
R4575 T16707 T16705 auxpass was,repressed
R4576 T16708 T16705 prep by,repressed
R4577 T16709 T16710 nummod 90,%
R4578 T16710 T16708 pobj %,by
R4579 T16711 T16705 prep in,repressed
R4580 T16712 T16713 compound HEK293T,cells
R4581 T16713 T16711 pobj cells,in
R4582 T16714 T16691 punct .,repressed
R4583 T16716 T16717 nsubj This,be
R4584 T16718 T16717 aux might,be
R4585 T16719 T16717 prep due,be
R4586 T16720 T16719 pcomp to,due
R4587 T16721 T16722 det the,difference
R4588 T16722 T16719 pobj difference,due
R4589 T16723 T16722 prep in,difference
R4590 T16724 T16725 compound transfection,efficiency
R4591 T16725 T16723 pobj efficiency,in
R4592 T16726 T16722 prep between,difference
R4593 T16727 T16728 det these,lines
R4594 T16728 T16726 pobj lines,between
R4595 T16729 T16728 compound cell,lines
R4596 T16730 T16717 punct .,be
R4597 T16732 T16733 det Another,possibility
R4598 T16733 T16734 nsubj possibility,is
R4599 T16735 T16736 mark that,is
R4600 T16736 T16734 ccomp is,is
R4601 T16737 T16738 amod intracellular,environment
R4602 T16738 T16736 nsubj environment,is
R4603 T16739 T16738 prep in,environment
R4604 T16740 T16741 compound Y79,cells
R4605 T16741 T16739 pobj cells,in
R4606 T16742 T16741 punct ", ",cells
R4607 T16743 T16744 det a,line
R4608 T16744 T16741 appos line,cells
R4609 T16745 T16744 compound retinoblastoma,line
R4610 T16746 T16744 compound cell,line
R4611 T16747 T16736 punct ", ",is
R4612 T16748 T16736 acomp insufficient,is
R4613 T16749 T16748 prep for,insufficient
R4614 T16750 T16749 pcomp recapitulating,for
R4615 T16751 T16752 amod developing,photoreceptors
R4616 T16752 T16750 dobj photoreceptors,recapitulating
R4617 T16753 T16734 punct .,is
R4618 T16755 T16756 prep In,address
R4619 T16757 T16758 det this,study
R4620 T16758 T16755 pobj study,In
R4621 T16759 T16756 punct ", ",address
R4622 T16760 T16756 nsubj we,address
R4623 T16761 T16756 aux did,address
R4624 T16762 T16756 neg not,address
R4625 T16763 T16764 det the,question
R4626 T16764 T16756 dobj question,address
R4627 T16765 T16766 mark whether,reflect
R4628 T16766 T16764 acl reflect,question
R4629 T16767 T16766 cc or,reflect
R4630 T16768 T16766 neg not,reflect
R4631 T16769 T16770 poss our,data
R4632 T16770 T16766 nsubj data,reflect
R4633 T16771 T16772 advmod in,vitro
R4634 T16772 T16770 amod vitro,data
R4635 T16773 T16774 amod native,repression
R4636 T16774 T16766 dobj repression,reflect
R4637 T16775 T16776 nmod mr,s
R4638 T16776 T16774 nmod s,repression
R4639 T16777 T16776 punct -,s
R4640 T16778 T16774 amod transcriptional,repression
R4641 T16779 T16780 advmod in,vivo
R4642 T16780 T16766 advmod vivo,reflect
R4643 T16781 T16756 punct .,address
R4644 T16783 T16784 advmod However,support
R4645 T16785 T16784 punct ", ",support
R4646 T16786 T16787 det these,experiments
R4647 T16787 T16784 nsubj experiments,support
R4648 T16788 T16789 advmod in,vitro
R4649 T16789 T16787 amod vitro,experiments
R4650 T16790 T16787 acl using,experiments
R4651 T16791 T16792 nmod HEK293T,cells
R4652 T16792 T16790 dobj cells,using
R4653 T16793 T16791 cc and,HEK293T
R4654 T16794 T16791 conj Y79,HEK293T
R4655 T16795 T16784 advmod strongly,support
R4656 T16796 T16797 poss our,hypothesis
R4657 T16797 T16784 dobj hypothesis,support
R4658 T16798 T16799 mark that,functions
R4659 T16799 T16797 acl functions,hypothesis
R4660 T16800 T16801 compound mr,s
R4661 T16801 T16799 nsubj s,functions
R4662 T16802 T16801 punct -,s
R4663 T16803 T16799 prep as,functions
R4664 T16804 T16805 det a,repressor
R4665 T16805 T16803 pobj repressor,as
R4666 T16806 T16805 amod transcriptional,repressor
R4667 T16807 T16799 prep in,functions
R4668 T16808 T16809 amod developing,photoreceptors
R4669 T16809 T16807 pobj photoreceptors,in
R4670 T16810 T16784 punct .,support
R4671 T16812 T16813 poss Our,results
R4672 T16813 T16814 nsubj results,suggested
R4673 T16815 T16816 mark that,required
R4674 T16816 T16814 ccomp required,suggested
R4675 T16817 T16818 det the,region
R4676 T16818 T16816 nsubjpass region,required
R4677 T16819 T16820 npadvmod C,terminal
R4678 T16820 T16818 amod terminal,region
R4679 T16821 T16820 punct -,terminal
R4680 T16822 T16818 prep of,region
R4681 T16823 T16824 compound mr,s
R4682 T16824 T16822 pobj s,of
R4683 T16825 T16824 punct -,s
R4684 T16826 T16827 punct (,463
R4685 T16827 T16818 parataxis 463,region
R4686 T16828 T16827 nmod amino,463
R4687 T16829 T16827 nmod acids,463
R4688 T16830 T16827 prep to,463
R4689 T16831 T16830 pobj 542,to
R4690 T16832 T16827 punct ),463
R4691 T16833 T16816 auxpass is,required
R4692 T16834 T16816 prep for,required
R4693 T16835 T16836 amod transcriptional,repression
R4694 T16836 T16834 pobj repression,for
R4695 T16837 T16836 prep of,repression
R4696 T16838 T16839 compound mr,s
R4697 T16839 T16837 pobj s,of
R4698 T16840 T16839 punct -,s
R4699 T16841 T16816 cc and,required
R4700 T16842 T16843 det the,domain
R4701 T16843 T16845 nsubj domain,appears
R4702 T16844 T16843 compound SAM,domain
R4703 T16845 T16816 conj appears,required
R4704 T16846 T16847 aux to,be
R4705 T16847 T16845 xcomp be,appears
R4706 T16848 T16847 acomp dispensable,be
R4707 T16849 T16848 prep for,dispensable
R4708 T16850 T16851 det this,activity
R4709 T16851 T16849 pobj activity,for
R4710 T16852 T16851 amod repressive,activity
R4711 T16853 T16814 punct .,suggested
R4712 T16855 T16856 det This,region
R4713 T16856 T16860 nsubjpass region,conserved
R4714 T16857 T16858 npadvmod C,terminal
R4715 T16858 T16856 amod terminal,region
R4716 T16859 T16858 punct -,terminal
R4717 T16861 T16856 prep of,region
R4718 T16862 T16863 compound mouse,s
R4719 T16863 T16861 pobj s,of
R4720 T16864 T16863 compound mr,s
R4721 T16865 T16863 punct -,s
R4722 T16866 T16860 auxpass is,conserved
R4723 T16867 T16860 advmod highly,conserved
R4724 T16868 T16860 prep among,conserved
R4725 T16869 T16868 pobj species,among
R4726 T16870 T16871 punct (,7F
R4727 T16871 T16860 parataxis 7F,conserved
R4728 T16872 T16871 compound Fig.,7F
R4729 T16873 T16871 punct ),7F
R4730 T16874 T16860 punct .,conserved
R4731 T16876 T16877 det The,identity
R4732 T16877 T16879 nsubj identity,was
R4733 T16878 T16877 compound sequence,identity
R4734 T16880 T16877 prep of,identity
R4735 T16881 T16882 det the,region
R4736 T16882 T16880 pobj region,of
R4737 T16883 T16884 nummod 93,%
R4738 T16884 T16879 attr %,was
R4739 T16885 T16884 punct ", ",%
R4740 T16886 T16887 nummod 73,%
R4741 T16887 T16884 conj %,%
R4742 T16888 T16887 punct ", ",%
R4743 T16889 T16890 nummod 41,%
R4744 T16890 T16887 conj %,%
R4745 T16891 T16890 cc and,%
R4746 T16892 T16893 nummod 40,%
R4747 T16893 T16890 conj %,%
R4748 T16894 T16884 prep for,%
R4749 T16895 T16894 pobj rat,for
R4750 T16896 T16895 punct ", ",rat
R4751 T16897 T16895 amod human,rat
R4752 T16898 T16895 punct ", ",rat
R4753 T16899 T16895 conj chick,rat
R4754 T16900 T16899 cc and,chick
R4755 T16901 T16899 conj zebrafish,chick
R4756 T16902 T16884 punct ", ",%
R4757 T16903 T16884 advmod respectively,%
R4758 T16904 T16905 punct (,1D
R4759 T16905 T16879 parataxis 1D,was
R4760 T16906 T16905 compound Fig.,1D
R4761 T16907 T16905 punct ),1D
R4762 T16908 T16879 punct .,was
R4763 T16910 T16911 nsubj This,suggests
R4764 T16912 T16911 advmod strongly,suggests
R4765 T16913 T16914 mark that,functions
R4767 T16915 T16916 det the,region
R4768 T16916 T16914 nsubj region,functions
R4769 T16917 T16918 npadvmod C,terminal
R4770 T16918 T16916 amod terminal,region
R4771 T16919 T16918 punct -,terminal
R4805 T16955 T16951 cc and,achieves
R4806 T16956 T16955 punct /,and
R4807 T16957 T16955 cc or,and
R4808 T16958 T16951 conj maintains,achieves
R4809 T16959 T16960 compound gene,repression
R4810 T16960 T16958 dobj repression,maintains
R4811 T16961 T16962 aux to,clarified
R4812 T16962 T16949 xcomp clarified,remains
R4813 T16963 T16962 auxpass be,clarified
R4814 T16964 T16962 prep in,clarified
R4815 T16965 T16966 det the,future
R4816 T16966 T16964 pobj future,in
R4817 T16967 T16936 punct .,contain
R4824 T16976 T16970 advcl functions,suggest
R4825 T16977 T16978 compound DBD,mrs
R4826 T16978 T16976 nsubj mrs,functions
R4827 T16979 T16978 punct -,mrs
R4828 T16980 T16976 prep as,functions
R4829 T16981 T16982 det a,repressor
R4830 T16982 T16980 pobj repressor,as
R4831 T16983 T16982 amod transcriptional,repressor
R4832 T16984 T16976 cc and,functions
R4833 T16985 T16986 mark that,is
R4834 T16986 T16976 conj is,functions
R4835 T16987 T16988 det the,activity
R4836 T16988 T16986 nsubj activity,is
R4837 T16989 T16988 compound repression,activity
R4838 T16990 T16988 prep of,activity
R4839 T16991 T16992 compound mr,s
R4840 T16992 T16990 pobj s,of
R4841 T16993 T16992 punct -,s
R4842 T16994 T16986 neg not,is
R4843 T16995 T16986 prep due,is
R4844 T16996 T16995 prep to,due
R4845 T16997 T16998 det a,interaction
R4846 T16998 T16996 pobj interaction,to
R4847 T16999 T16998 amod homophilic,interaction
R4848 T17000 T16998 prep through,interaction
R4849 T17001 T17002 poss its,domain
R4850 T17002 T17000 pobj domain,through
R4851 T17003 T17002 compound SAM,domain
R4852 T17004 T16986 cc but,is
R4853 T17005 T16986 conj to,is
R4854 T17006 T17007 det the,region
R4855 T17007 T17005 pobj region,to
R4856 T17008 T17009 npadvmod C,terminal
R4857 T17009 T17007 amod terminal,region
R4858 T17010 T17009 punct -,terminal
R4859 T17011 T17012 punct (,463
R4860 T17012 T17007 parataxis 463,region
R4861 T17013 T17012 nmod amino,463
R4862 T17014 T17012 nmod acids,463
R4863 T17015 T17012 prep to,463
R4864 T17016 T17015 pobj 542,to
R4865 T17017 T17012 punct ),463
R4866 T17018 T16970 punct .,suggest
R4875 T20693 T20694 prep In,identified
R4876 T20695 T20696 det the,study
R4877 T20696 T20693 pobj study,In
R4878 T20697 T20696 amod present,study
R4879 T20698 T20694 punct ", ",identified
R4880 T20699 T20694 nsubj we,identified
R4881 T20700 T20701 det a,gene
R4882 T20701 T20694 dobj gene,identified
R4883 T20702 T20701 amod novel,gene
R4884 T20703 T20701 punct ", ",gene
R4885 T20704 T20705 compound mr,s
R4886 T20705 T20701 appos s,gene
R4887 T20706 T20705 punct -,s
R4888 T20707 T20701 punct ", ",gene
R4889 T20708 T20709 dep which,expressed
R4890 T20709 T20701 relcl expressed,gene
R4891 T20710 T20709 auxpass is,expressed
R4892 T20711 T20709 advmod predominantly,expressed
R4893 T20712 T20709 prep in,expressed
R4894 T20713 T20714 amod retinal,photoreceptors
R4895 T20714 T20712 pobj photoreceptors,in
R4896 T20715 T20714 cc and,photoreceptors
R4897 T20716 T20717 det the,gland
R4898 T20717 T20714 conj gland,photoreceptors
R4899 T20718 T20717 amod pineal,gland
R4900 T20719 T20694 punct .,identified
R4901 T20721 T20722 det The,peak
R4902 T20722 T20723 nsubj peak,is
R4903 T20724 T20722 prep of,peak
R4904 T20725 T20726 compound mr,s
R4905 T20726 T20728 compound s,expression
R4906 T20727 T20726 punct -,s
R4907 T20728 T20724 pobj expression,of
R4908 T20729 T20728 prep in,expression
R4909 T20730 T20731 det the,retina
R4910 T20731 T20729 pobj retina,in
R4911 T20732 T20731 amod developing,retina
R4912 T20733 T20723 prep around,is
R4913 T20734 T20733 pobj P6,around
R4914 T20735 T20723 punct .,is
R4915 T20737 T20738 det This,pattern
R4916 T20738 T20740 nsubj pattern,correlates
R4917 T20739 T20738 compound expression,pattern
R4918 T20741 T20740 prep with,correlates
R4919 T20742 T20743 det the,increase
R4920 T20743 T20741 pobj increase,with
R4921 T20744 T20743 amod rapid,increase
R4922 T20745 T20743 prep of,increase
R4923 T20746 T20745 pobj Crx,of
R4924 T20747 T20746 punct ", ",Crx
R4925 T20748 T20746 conj rhodopsin,Crx
R4926 T20749 T20748 cc and,rhodopsin
R4927 T20750 T20751 amod other,genes
R4928 T20751 T20748 conj genes,rhodopsin
R4929 T20752 T20751 compound photoreceptor,genes
R4930 T20753 T20743 prep around,increase
R4931 T20754 T20753 pobj P6,around
R4932 T20755 T20756 punct -,P8
R4933 T20756 T20754 prep P8,P6
R4934 T20757 T20740 punct .,correlates
R4935 T20759 T20760 prep Around,becomes
R4936 T20761 T20759 pobj P6,Around
R4937 T20762 T20760 punct ", ",becomes
R4938 T20763 T20764 det the,layer
R4939 T20764 T20760 nsubj layer,becomes
R4940 T20765 T20764 amod outer,layer
R4941 T20766 T20764 compound plexiform,layer
R4942 T20767 T20760 acomp visible,becomes
R4943 T20768 T20760 cc and,becomes
R4944 T20769 T20770 det the,layer
R4945 T20770 T20772 nsubj layer,separates
R4946 T20771 T20770 amod outer,layer
R4947 T20772 T20760 conj separates,becomes
R4948 T20773 T20770 prep of,layer
R4949 T20774 T20773 pobj retina,of
R4950 T20775 T20772 prep into,separates
R4951 T20776 T20777 nummod two,layers
R4952 T20777 T20775 pobj layers,into
R4953 T20778 T20777 punct ", ",layers
R4954 T20779 T20777 appos ONL,layers
R4955 T20780 T20779 cc and,ONL
R4956 T20781 T20779 conj INL,ONL
R4957 T20782 T20760 punct .,becomes
R4958 T20784 T20785 prep At,begin
R4959 T20786 T20787 det the,time
R4960 T20787 T20784 pobj time,At
R4961 T20788 T20787 amod same,time
R4962 T20789 T20785 punct ", ",begin
R4963 T20790 T20785 nsubj photoreceptors,begin
R4964 T20791 T20792 aux to,undergo
R4965 T20792 T20785 xcomp undergo,begin
R4966 T20793 T20794 amod terminal,differentiation
R4967 T20794 T20792 dobj differentiation,undergo
R4968 T20795 T20792 punct ", ",undergo
R4969 T20796 T20792 advcl forming,undergo
R4970 T20797 T20798 det the,segment
R4971 T20798 T20796 dobj segment,forming
R4972 T20799 T20798 amod outer,segment
R4973 T20800 T20785 punct .,begin
R4974 T20802 T20803 nsubj We,hypothesized
R4975 T20804 T20803 advmod therefore,hypothesized
R4976 T20805 T20806 mark that,is
R4977 T20806 T20803 ccomp is,hypothesized
R4978 T20807 T20808 compound mr,s
R4979 T20808 T20806 nsubj s,is
R4980 T20809 T20808 punct -,s
R4981 T20810 T20811 det a,molecule
R4982 T20811 T20806 attr molecule,is
R4983 T20812 T20811 amod key,molecule
R4984 T20813 T20811 prep in,molecule
R4985 T20814 T20815 det the,development
R4986 T20815 T20813 pobj development,in
R4987 T20816 T20815 amod late,development
R4988 T20817 T20815 prep of,development
R4989 T20818 T20817 pobj photoreceptors,of
R4990 T20819 T20803 punct .,hypothesized
R4991 T20821 T20822 nsubj We,reported
R4992 T20823 T20822 advmod previously,reported
R4993 T20824 T20825 mark that,have
R4994 T20825 T20822 advcl have,reported
R4995 T20826 T20825 nsubj Otx2,have
R4996 T20827 T20826 cc and,Otx2
R4997 T20828 T20826 conj Crx,Otx2
R4998 T20829 T20830 det a,role
R4999 T20830 T20825 dobj role,have
R5000 T20831 T20830 amod critical,role
R5001 T20832 T20830 prep in,role
R5002 T20833 T20834 compound photoreceptor,development
R5003 T20834 T20832 pobj development,in
R5004 T20835 T20825 cc and,have
R5005 T20836 T20837 mark that,regulates
R5006 T20837 T20825 conj regulates,have
R5007 T20838 T20837 nsubj Otx2,regulates
R5008 T20839 T20837 advmod directly,regulates
R5009 T20840 T20841 compound Crx,transcription
R5010 T20841 T20837 dobj transcription,regulates
R5011 T20842 T20843 punct [,9
R5012 T20843 T20822 parataxis 9,reported
R5013 T20844 T20843 nummod 3,9
R5014 T20845 T20843 punct ",",9
R5015 T20846 T20843 punct ],9
R5016 T20847 T20822 punct .,reported
R5017 T20849 T20850 advmod In,situ
R5018 T20850 T20851 amod situ,hybridization
R5019 T20851 T20852 nsubj hybridization,showed
R5020 T20853 T20851 cc and,hybridization
R5021 T20854 T20855 compound RT,PCR
R5022 T20855 T20851 conj PCR,hybridization
R5023 T20856 T20855 punct -,PCR
R5024 T20857 T20858 amod significant,reduction
R5025 T20858 T20852 dobj reduction,showed
R5026 T20859 T20858 prep of,reduction
R5027 T20860 T20861 compound mr,s
R5028 T20861 T20863 compound s,signal
R5029 T20862 T20861 punct -,s
R5030 T20863 T20859 pobj signal,of
R5031 T20864 T20852 prep in,showed
R5032 T20865 T20866 det the,KO
R5033 T20866 T20864 pobj KO,in
R5034 T20867 T20866 compound Crx,KO
R5035 T20868 T20866 appos retina,KO
R5036 T20869 T20868 cc and,retina
R5037 T20870 T20871 amod pineal,gland
R5038 T20871 T20868 conj gland,retina
R5039 T20872 T20852 punct .,showed
R5040 T20874 T20875 advmod Furthermore,demonstrated
R5041 T20876 T20875 punct ", ",demonstrated
R5042 T20877 T20878 det the,assay
R5043 T20878 T20875 nsubj assay,demonstrated
R5044 T20879 T20878 compound luciferase,assay
R5045 T20880 T20881 mark that,upregulate
R5046 T20881 T20875 ccomp upregulate,demonstrated
R5047 T20882 T20881 nsubj Otx2,upregulate
R5048 T20883 T20882 cc and,Otx2
R5049 T20884 T20882 conj Crx,Otx2
R5050 T20885 T20881 aux may,upregulate
R5051 T20886 T20881 advmod directly,upregulate
R5052 T20887 T20888 det the,transcription
R5053 T20888 T20881 dobj transcription,upregulate
R5054 T20889 T20888 prep of,transcription
R5055 T20890 T20891 compound mr,s
R5056 T20891 T20889 pobj s,of
R5057 T20892 T20891 punct -,s
R5058 T20893 T20881 prep in,upregulate
R5059 T20894 T20895 amod mammalian,cells
R5060 T20895 T20893 pobj cells,in
R5061 T20896 T20875 punct .,demonstrated
R5062 T20898 T20899 prep In,are
R5063 T20900 T20901 amod retinal,cells
R5064 T20901 T20898 pobj cells,In
R5065 T20902 T20901 compound photoreceptor,cells
R5066 T20903 T20899 punct ", ",are
R5067 T20904 T20905 det the,transcripts
R5068 T20905 T20899 nsubj transcripts,are
R5069 T20906 T20905 compound Otx2,transcripts
R5070 T20907 T20899 neg not,are
R5071 T20908 T20909 advmod highly,expressed
R5072 T20909 T20899 acomp expressed,are
R5073 T20910 T20899 prep at,are
R5074 T20911 T20910 pobj P6,at
R5075 T20912 T20913 punct -,P9
R5076 T20913 T20911 prep P9,P6
R5077 T20914 T20899 punct ", ",are
R5078 T20915 T20916 mark while,detected
R5079 T20916 T20899 advcl detected,are
R5080 T20917 T20918 det the,transcripts
R5081 T20918 T20916 nsubjpass transcripts,detected
R5082 T20919 T20918 compound Crx,transcripts
R5083 T20920 T20916 auxpass are,detected
R5084 T20921 T20916 advmod strongly,detected
R5085 T20922 T20916 prep around,detected
R5086 T20923 T20922 pobj P6,around
R5087 T20924 T20899 punct .,are
R5088 T20926 T20927 advmod Therefore,suggest
R5089 T20928 T20927 punct ", ",suggest
R5090 T20929 T20930 poss our,results
R5091 T20930 T20927 nsubj results,suggest
R5092 T20931 T20927 advmod strongly,suggest
R5093 T20932 T20933 mark that,regulated
R5094 T20933 T20927 ccomp regulated,suggest
R5095 T20934 T20935 compound mr,s
R5096 T20935 T20937 compound s,transcription
R5097 T20936 T20935 punct -,s
R5098 T20937 T20933 nsubjpass transcription,regulated
R5099 T20938 T20933 auxpass is,regulated
R5100 T20939 T20933 advmod directly,regulated
R5101 T20940 T20933 agent by,regulated
R5102 T20941 T20940 pobj Crx,by
R5103 T20942 T20927 punct .,suggest
R5104 T20944 T20945 prep In,affect
R5105 T20946 T20947 det the,study
R5106 T20947 T20944 pobj study,In
R5107 T20948 T20947 amod present,study
R5108 T20949 T20945 punct ", ",affect
R5109 T20950 T20945 nsubj Nrl,affect
R5110 T20951 T20950 punct ", ",Nrl
R5111 T20952 T20953 det a,factor
R5112 T20953 T20950 appos factor,Nrl
R5113 T20954 T20955 npadvmod photoreceptor,specific
R5114 T20955 T20953 amod specific,factor
R5115 T20956 T20955 punct -,specific
R5116 T20957 T20953 compound transcription,factor
R5117 T20958 T20959 dep that,expressed
R5118 T20959 T20953 relcl expressed,factor
R5119 T20960 T20959 auxpass is,expressed
R5120 T20961 T20959 advmod highly,expressed
R5121 T20962 T20959 prep in,expressed
R5122 T20963 T20962 pobj photoreceptors,in
R5123 T20964 T20959 prep at,expressed
R5124 T20965 T20966 det the,stage
R5125 T20966 T20964 pobj stage,at
R5126 T20967 T20966 amod postnatal,stage
R5127 T20968 T20945 punct ", ",affect
R5128 T20969 T20945 aux did,affect
R5129 T20970 T20945 neg not,affect
R5130 T20971 T20972 det the,transcription
R5131 T20972 T20945 dobj transcription,affect
R5132 T20973 T20972 prep of,transcription
R5133 T20974 T20975 compound mr,s
R5134 T20975 T20973 pobj s,of
R5135 T20976 T20975 punct -,s
R5136 T20977 T20945 punct .,affect
R5137 T20979 T20980 det This,finding
R5138 T20980 T20981 nsubj finding,is
R5139 T20982 T20981 advmod actually,is
R5140 T20983 T20981 acomp consistent,is
R5141 T20984 T20983 prep with,consistent
R5142 T20985 T20986 det the,analysis
R5143 T20986 T20984 pobj analysis,with
R5144 T20987 T20986 prep of,analysis
R5145 T20988 T20989 det the,mouse
R5146 T20989 T20987 pobj mouse,of
R5147 T20990 T20991 compound Nrl,KO
R5148 T20991 T20989 compound KO,mouse
R5149 T20992 T20993 dep which,reported
R5150 T20993 T20989 relcl reported,mouse
R5151 T20994 T20993 auxpass was,reported
R5152 T20995 T20993 advmod recently,reported
R5153 T20996 T20997 punct [,40
R5154 T20997 T20981 parataxis 40,is
R5155 T20998 T20997 punct ],40
R5156 T20999 T20981 punct .,is
R5157 T21001 T21002 det The,profiles
R5158 T21002 T21004 nsubjpass profiles,analyzed
R5159 T21003 T21002 compound expression,profiles
R5160 T21005 T21002 prep of,profiles
R5161 T21006 T21007 amod wild,type
R5162 T21007 T21009 nmod type,retinas
R5163 T21008 T21007 punct -,type
R5164 T21009 T21005 pobj retinas,of
R5165 T21010 T21007 cc and,type
R5166 T21011 T21012 compound Nrl,KO
R5167 T21012 T21007 conj KO,type
R5168 T21013 T21002 prep at,profiles
R5169 T21014 T21013 pobj P2,at
R5170 T21015 T21014 punct ", ",P2
R5171 T21016 T21014 conj P10,P2
R5172 T21017 T21016 cc and,P10
R5173 T21018 T21019 nummod 2,months
R5174 T21019 T21016 conj months,P10
R5175 T21020 T21004 auxpass were,analyzed
R5176 T21021 T21004 cc and,analyzed
R5177 T21022 T21023 compound mr,s
R5178 T21023 T21025 nsubjpass s,included
R5179 T21024 T21023 punct -,s
R5180 T21025 T21004 conj included,analyzed
R5181 T21026 T21025 auxpass was,included
R5182 T21027 T21025 neg not,included
R5183 T21028 T21025 prep in,included
R5184 T21029 T21030 nummod 161,genes
R5185 T21030 T21028 pobj genes,in
R5186 T21031 T21032 advmod differentially,expressed
R5187 T21032 T21030 amod expressed,genes
R5188 T21033 T21025 prep in,included
R5189 T21034 T21035 det the,retina
R5190 T21035 T21033 pobj retina,in
R5191 T21036 T21037 compound Nrl,KO
R5192 T21037 T21035 compound KO,retina
R5193 T21038 T21025 punct .,included
R5194 T21040 T21041 amod Previous,reports
R5195 T21041 T21042 nsubj reports,suggested
R5196 T21043 T21044 mark that,is
R5197 T21044 T21042 ccomp is,suggested
R5198 T21045 T21046 det the,domain
R5199 T21046 T21044 nsubj domain,is
R5200 T21047 T21046 compound SAM,domain
R5201 T21048 T21049 det a,module
R5202 T21049 T21044 attr module,is
R5203 T21050 T21051 compound protein,protein
R5204 T21051 T21049 compound protein,module
R5205 T21052 T21051 punct -,protein
R5206 T21053 T21049 compound interaction,module
R5207 T21054 T21042 punct .,suggested
R5208 T21056 T21057 det The,domain
R5209 T21057 T21059 nsubjpass domain,related
R5210 T21058 T21057 compound SAM,domain
R5211 T21060 T21057 prep of,domain
R5212 T21061 T21062 det the,protein
R5213 T21062 T21060 pobj protein,of
R5214 T21063 T21064 compound mr,s
R5215 T21064 T21062 compound s,protein
R5216 T21065 T21064 punct -,s
R5217 T21066 T21059 auxpass is,related
R5218 T21067 T21059 advmod closely,related
R5219 T21068 T21059 prep to,related
R5220 T21069 T21068 pobj that,to
R5221 T21070 T21069 prep of,that
R5222 T21071 T21070 pobj ph,of
R5223 T21072 T21071 cc and,ph
R5224 T21073 T21071 conj TEL,ph
R5225 T21074 T21071 punct ", ",ph
R5226 T21075 T21076 poss whose,domains
R5227 T21076 T21078 dep domains,form
R5228 T21077 T21076 compound SAM,domains
R5229 T21078 T21071 relcl form,ph
R5230 T21079 T21078 aux can,form
R5231 T21080 T21081 det a,structure
R5232 T21081 T21078 dobj structure,form
R5233 T21082 T21081 amod helical,structure
R5234 T21083 T21081 punct ", ",structure
R5235 T21084 T21081 nmod head,structure
R5236 T21085 T21084 punct -,head
R5237 T21086 T21084 prep to,head
R5238 T21087 T21086 punct -,to
R5239 T21088 T21086 pobj tail,to
R5240 T21089 T21081 amod polymeric,structure
R5241 T21090 T21078 cc and,form
R5242 T21091 T21078 conj mediate,form
R5243 T21092 T21093 det the,formation
R5244 T21093 T21091 dobj formation,mediate
R5245 T21094 T21093 prep of,formation
R5246 T21095 T21096 det a,structure
R5247 T21096 T21094 pobj structure,of
R5248 T21097 T21098 amod higher,order
R5249 T21098 T21096 compound order,structure
R5250 T21099 T21096 compound chromatin,structure
R5251 T21100 T21059 punct .,related
R5252 T21102 T21103 aux To,characterize
R5253 T21103 T21104 advcl characterize,performed
R5254 T21105 T21106 det the,function
R5255 T21106 T21103 dobj function,characterize
R5256 T21107 T21106 amod biochemical,function
R5257 T21108 T21106 prep of,function
R5258 T21109 T21110 compound mr,s
R5259 T21110 T21108 pobj s,of
R5260 T21111 T21110 punct -,s
R5261 T21112 T21104 punct ", ",performed
R5262 T21113 T21104 nsubj we,performed
R5263 T21114 T21115 nmod yeast,screening
R5264 T21115 T21104 dobj screening,performed
R5265 T21116 T21117 nummod two,hybrid
R5266 T21117 T21115 compound hybrid,screening
R5267 T21118 T21117 punct -,hybrid
R5268 T21119 T21104 advcl using,performed
R5269 T21120 T21121 amod full,length
R5270 T21121 T21123 compound length,s
R5271 T21122 T21121 punct -,length
R5272 T21123 T21119 dobj s,using
R5273 T21124 T21123 compound mr,s
R5274 T21125 T21123 punct -,s
R5275 T21126 T21119 prep as,using
R5276 T21127 T21128 det the,bait
R5277 T21128 T21126 pobj bait,as
R5278 T21129 T21104 punct .,performed
R5279 T21131 T21132 prep As,were
R5280 T21133 T21134 det a,result
R5281 T21134 T21131 pobj result,As
R5282 T21135 T21132 punct ", ",were
R5283 T21136 T21137 det the,clones
R5284 T21137 T21132 nsubj clones,were
R5285 T21138 T21139 advmod most,frequent
R5286 T21139 T21137 amod frequent,clones
R5287 T21140 T21137 amod positive,clones
R5288 T21141 T21142 punct (,5
R5289 T21142 T21137 parataxis 5,clones
R5290 T21143 T21144 punct /,28
R5291 T21144 T21142 prep 28,5
R5292 T21145 T21142 punct ),5
R5293 T21146 T21137 prep in,clones
R5294 T21147 T21148 det the,screening
R5295 T21148 T21146 pobj screening,in
R5296 T21173 T21174 poss its,domain
R5297 T21149 T21150 det the,fragments
R5298 T21174 T21172 pobj domain,through
R5299 T21150 T21132 attr fragments,were
R5300 T21175 T21174 compound SAM,domain
R5301 T21176 T21163 punct .,suggests
R5302 T21151 T21150 compound cDNA,fragments
R5303 T21178 T21179 det An,assay
R5304 T21152 T21150 acl containing,fragments
R5305 T21179 T21181 nsubj assay,demonstrated
R5306 T21180 T21179 compound immunoprecipitation,assay
R5307 T21153 T21154 det the,domain
R5308 T21182 T21179 punct ", ",assay
R5309 T21154 T21152 dobj domain,containing
R5310 T21183 T21179 acl using,assay
R5311 T21184 T21185 nummod two,mutants
R5312 T21185 T21183 dobj mutants,using
R5313 T21155 T21154 compound SAM,domain
R5314 T21186 T21187 npadvmod site,directed
R5315 T21187 T21185 amod directed,mutants
R5316 T21188 T21187 punct -,directed
R5317 T21156 T21154 prep of,domain
R5318 T21189 T21185 prep of,mutants
R5319 T21190 T21191 det the,domain
R5320 T21157 T21158 compound mr,s
R5321 T21191 T21189 pobj domain,of
R5322 T21192 T21191 compound SAM,domain
R5323 T21193 T21191 prep of,domain
R5324 T21158 T21156 pobj s,of
R5325 T21194 T21195 compound mr,s
R5326 T21195 T21193 pobj s,of
R5327 T21159 T21158 punct -,s
R5328 T21196 T21195 punct -,s
R5329 T21160 T21132 punct .,were
R5330 T21162 T21163 nsubj This,suggests
R5331 T21197 T21181 punct ", ",demonstrated
R5332 T21198 T21199 mark that,associate
R5333 T21164 T21163 advmod strongly,suggests
R5334 T21199 T21181 ccomp associate,demonstrated
R5335 T21200 T21201 det the,protein
R5336 T21201 T21199 nsubj protein,associate
R5337 T21202 T21203 compound mr,s
R5338 T21203 T21201 compound s,protein
R5339 T21165 T21166 mark that,associates
R5340 T21204 T21203 punct -,s
R5341 T21166 T21163 ccomp associates,suggests
R5342 T21205 T21199 aux can,associate
R5343 T21206 T21199 dep self,associate
R5344 T21207 T21199 punct -,associate
R5345 T21208 T21199 prep through,associate
R5346 T21209 T21210 poss its,domain
R5347 T21167 T21168 compound mr,s
R5348 T21210 T21208 pobj domain,through
R5349 T21211 T21210 compound SAM,domain
R5350 T21168 T21166 nsubj s,associates
R5351 T21212 T21199 prep in,associate
R5352 T21213 T21214 amod mammalian,cells
R5353 T21214 T21212 pobj cells,in
R5354 T21169 T21168 punct -,s
R5355 T21215 T21181 punct .,demonstrated
R5356 T21170 T21166 dep self,associates
R5357 T21217 T21218 mark While,address
R5358 T21218 T21222 advcl address,suggests
R5359 T21171 T21166 punct -,associates
R5360 T21219 T21218 nsubj we,address
R5361 T21220 T21218 aux did,address
R5362 T21221 T21218 neg not,address
R5363 T21172 T21166 prep through,associates
R5364 T21223 T21224 det the,question
R5365 T21224 T21218 dobj question,address
R5366 T21279 T21276 prep along,spreading
R5367 T21225 T21226 mark whether,forms
R5368 T21226 T21224 acl forms,question
R5369 T21227 T21228 det the,domain
R5370 T21228 T21226 nsubj domain,forms
R5371 T21280 T21281 det the,chromatin
R5372 T21229 T21228 compound SAM,domain
R5373 T21230 T21228 prep of,domain
R5374 T21281 T21279 pobj chromatin,along
R5375 T21231 T21232 compound mr,s
R5376 T21232 T21230 pobj s,of
R5377 T21233 T21232 punct -,s
R5378 T21282 T21272 advcl similar,mediate
R5379 T21234 T21235 det a,structure
R5380 T21235 T21226 dobj structure,forms
R5381 T21283 T21282 prep to,similar
R5382 T21236 T21235 amod polymeric,structure
R5383 T21237 T21218 prep in,address
R5384 T21238 T21239 det the,study
R5385 T21284 T21283 pobj ph,to
R5386 T21239 T21237 pobj study,in
R5387 T21240 T21239 amod present,study
R5388 T21241 T21222 punct ", ",suggests
R5389 T21242 T21243 det the,analysis
R5390 T21243 T21222 nsubj analysis,suggests
R5391 T21244 T21243 amod phylogenetic,analysis
R5392 T21285 T21284 cc and,ph
R5393 T21245 T21243 prep of,analysis
R5394 T21286 T21285 punct /,and
R5395 T21246 T21247 compound SAM,domain
R5396 T21247 T21245 pobj domain,of
R5397 T21248 T21247 prep of,domain
R5398 T21249 T21250 compound mr,s
R5399 T21287 T21285 cc or,and
R5400 T21250 T21248 pobj s,of
R5401 T21251 T21250 punct -,s
R5402 T21252 T21250 cc and,s
R5403 T21288 T21284 conj TEL,ph
R5404 T21253 T21254 amod other,molecules
R5405 T21254 T21250 conj molecules,s
R5406 T21289 T21222 punct .,suggests
R5407 T21255 T21256 compound SAM,domain
R5408 T21256 T21257 npadvmod domain,containing
R5409 T21257 T21254 amod containing,molecules
R5410 T21291 T21292 mark Although,demonstrated
R5411 T21258 T21257 punct -,containing
R5412 T21259 T21260 mark that,form
R5413 T21260 T21222 ccomp form,suggests
R5414 T21261 T21262 compound mr,s
R5415 T21292 T21299 advcl demonstrated,fit
R5416 T21262 T21260 nsubj s,form
R5417 T21263 T21262 punct -,s
R5418 T21293 T21294 poss our,results
R5419 T21264 T21260 aux can,form
R5420 T21265 T21266 nmod head,polymer
R5421 T21266 T21260 dobj polymer,form
R5422 T21294 T21292 nsubj results,demonstrated
R5423 T21267 T21265 punct -,head
R5424 T21268 T21265 prep to,head
R5425 T21295 T21294 prep in,results
R5426 T21269 T21268 punct -,to
R5427 T21270 T21268 pobj tail,to
R5428 T21271 T21260 cc and,form
R5429 T21296 T21297 det the,assay
R5430 T21272 T21260 conj mediate,form
R5431 T21273 T21274 compound gene,silencing
R5432 T21274 T21272 dobj silencing,mediate
R5433 T21297 T21295 pobj assay,in
R5434 T21275 T21272 prep by,mediate
R5435 T21276 T21275 pcomp spreading,by
R5436 T21298 T21297 compound immunoprecipitation,assay
R5437 T21277 T21278 amod repressive,complexes
R5438 T21278 T21276 dobj complexes,spreading
R5439 T21300 T21301 mark that,interact
R5440 T21301 T21292 ccomp interact,demonstrated
R5441 T21385 T21384 punct -,specific
R5442 T21302 T21303 det the,constructs
R5443 T21386 T21387 npadvmod DNA,binding
R5444 T21387 T21382 amod binding,domain
R5445 T21303 T21301 nsubj constructs,interact
R5446 T21388 T21389 punct ", ",domain
R5447 T21389 T21382 appos domain,domain
R5448 T21304 T21305 npadvmod N,terminal
R5449 T21390 T21389 advmod namely,domain
R5450 T21391 T21389 det the,domain
R5451 T21305 T21303 amod terminal,constructs
R5452 T21392 T21389 compound ETS,domain
R5453 T21393 T21379 punct ", ",contains
R5454 T21394 T21379 cc and,contains
R5455 T21306 T21305 punct -,terminal
R5456 T21395 T21379 conj binds,contains
R5457 T21396 T21395 prep to,binds
R5458 T21307 T21303 acl lacking,constructs
R5459 T21397 T21398 amod specific,sites
R5460 T21398 T21396 pobj sites,to
R5461 T21399 T21395 prep via,binds
R5462 T21308 T21309 det a,domain
R5463 T21400 T21401 poss its,domain
R5464 T21401 T21399 pobj domain,via
R5465 T21402 T21401 compound ETS,domain
R5466 T21309 T21307 dobj domain,lacking
R5467 T21403 T21404 punct [,41
R5468 T21404 T21377 parataxis 41,indicated
R5469 T21310 T21309 compound SAM,domain
R5470 T21405 T21404 punct ],41
R5471 T21406 T21377 punct .,indicated
R5472 T21311 T21301 advmod still,interact
R5473 T21408 T21409 nsubj TEL,serve
R5474 T21312 T21301 prep with,interact
R5475 T21410 T21409 aux could,serve
R5476 T21411 T21412 aux to,nucleate
R5477 T21412 T21409 xcomp nucleate,serve
R5478 T21313 T21314 det each,other
R5479 T21413 T21414 det a,polymer
R5480 T21414 T21412 dobj polymer,nucleate
R5481 T21314 T21312 pobj other,with
R5482 T21415 T21414 punct ", ",polymer
R5483 T21416 T21417 dep which,spread
R5484 T21417 T21414 relcl spread,polymer
R5485 T21315 T21299 punct ", ",fit
R5486 T21418 T21417 aux would,spread
R5487 T21419 T21417 prep by,spread
R5488 T21420 T21419 pobj oligomerization,by
R5489 T21316 T21317 det these,results
R5490 T21421 T21420 prep of,oligomerization
R5491 T21317 T21299 nsubj results,fit
R5492 T21422 T21423 det the,domain
R5493 T21318 T21299 aux do,fit
R5494 T21423 T21421 pobj domain,of
R5495 T21424 T21423 compound SAM,domain
R5496 T21319 T21299 neg not,fit
R5497 T21425 T21409 punct .,serve
R5498 T21320 T21299 prep into,fit
R5499 T21427 T21428 prep In,contain
R5500 T21429 T21427 pobj contrast,In
R5501 T21321 T21322 det the,model
R5502 T21430 T21429 prep to,contrast
R5503 T21431 T21430 pobj TEL,to
R5504 T21432 T21428 punct ", ",contain
R5505 T21322 T21320 pobj model,into
R5506 T21433 T21428 nsubj ph,contain
R5507 T21434 T21428 aux does,contain
R5508 T21323 T21322 nmod head,model
R5509 T21435 T21428 neg not,contain
R5510 T21436 T21437 det an,motif
R5511 T21437 T21428 dobj motif,contain
R5512 T21324 T21323 punct -,head
R5513 T21438 T21437 amod obvious,motif
R5514 T21439 T21440 npadvmod sequence,specific
R5515 T21440 T21437 amod specific,motif
R5516 T21325 T21323 prep to,head
R5517 T21441 T21440 punct -,specific
R5518 T21442 T21443 npadvmod DNA,binding
R5519 T21443 T21437 amod binding,motif
R5520 T21326 T21325 punct -,to
R5521 T21444 T21445 punct (,16
R5522 T21445 T21428 parataxis 16,contain
R5523 T21446 T21445 punct ),16
R5524 T21327 T21325 pobj tail,to
R5525 T21447 T21428 punct .,contain
R5526 T21449 T21450 advmod Therefore,require
R5527 T21328 T21322 compound polymer,model
R5528 T21451 T21450 punct ", ",require
R5529 T21452 T21453 poss its,binding
R5530 T21329 T21299 punct .,fit
R5531 T21453 T21450 nsubj binding,require
R5532 T21454 T21453 amod initial,binding
R5533 T21331 T21332 nsubj We,exclude
R5534 T21333 T21332 aux can,exclude
R5535 T21455 T21453 prep to,binding
R5536 T21334 T21332 neg not,exclude
R5537 T21456 T21457 det the,template
R5538 T21457 T21455 pobj template,to
R5539 T21458 T21450 aux may,require
R5540 T21335 T21336 det the,possibility
R5541 T21459 T21460 compound protein,protein
R5542 T21460 T21462 compound protein,interactions
R5543 T21461 T21460 punct -,protein
R5544 T21336 T21332 dobj possibility,exclude
R5545 T21462 T21450 dobj interactions,require
R5546 T21463 T21462 prep with,interactions
R5547 T21464 T21465 amod other,repressors
R5548 T21465 T21463 pobj repressors,with
R5549 T21337 T21338 mark that,is
R5550 T21466 T21467 npadvmod sequence,specific
R5551 T21467 T21465 amod specific,repressors
R5552 T21468 T21467 punct -,specific
R5553 T21338 T21336 acl is,possibility
R5554 T21469 T21465 amod transcriptional,repressors
R5555 T21470 T21450 punct .,require
R5556 T21339 T21340 det the,interaction
R5557 T21472 T21473 det The,repressors
R5558 T21473 T21479 nsubj repressors,have
R5559 T21340 T21338 nsubj interaction,is
R5560 T21474 T21473 nmod segmentation,repressors
R5561 T21475 T21476 npadvmod gene,encoding
R5562 T21476 T21473 amod encoding,repressors
R5563 T21341 T21340 amod resulting,interaction
R5564 T21477 T21476 punct -,encoding
R5565 T21478 T21473 amod transcriptional,repressors
R5566 T21342 T21343 compound protein,protein
R5567 T21480 T21481 amod such,as
R5568 T21481 T21473 prep as,repressors
R5569 T21482 T21481 pobj Hunchback,as
R5570 T21343 T21340 compound protein,interaction
R5571 T21483 T21484 det a,role
R5572 T21484 T21479 dobj role,have
R5573 T21344 T21343 punct -,protein
R5574 T21485 T21484 prep in,role
R5575 T21486 T21485 pcomp recruiting,in
R5576 T21487 T21488 nmod SAM,proteins
R5577 T21345 T21340 prep of,interaction
R5578 T21488 T21486 dobj proteins,recruiting
R5579 T21489 T21490 npadvmod domain,containing
R5580 T21346 T21347 compound mr,s
R5581 T21347 T21345 pobj s,of
R5582 T21348 T21347 punct -,s
R5583 T21349 T21350 det an,artifact
R5584 T21350 T21338 attr artifact,is
R5585 T21490 T21488 amod containing,proteins
R5586 T21491 T21490 punct -,containing
R5587 T21492 T21488 compound PcG,proteins
R5588 T21351 T21350 prep of,artifact
R5589 T21493 T21488 punct ", ",proteins
R5590 T21494 T21495 dep which,spread
R5591 T21495 T21488 relcl spread,proteins
R5592 T21352 T21353 det the,conditions
R5593 T21496 T21495 aux can,spread
R5594 T21497 T21495 prep along,spread
R5595 T21498 T21499 det the,template
R5596 T21353 T21351 pobj conditions,of
R5597 T21499 T21497 pobj template,along
R5598 T21500 T21495 prep via,spread
R5599 T21501 T21500 pobj polymerization,via
R5600 T21502 T21503 punct [,42
R5601 T21503 T21479 parataxis 42,have
R5602 T21504 T21503 punct ],42
R5603 T21354 T21353 compound overexpression,conditions
R5604 T21505 T21479 punct .,have
R5605 T21507 T21508 mark Since,contain
R5606 T21355 T21332 punct .,exclude
R5607 T21508 T21514 advcl contain,suppose
R5608 T21509 T21510 compound mr,s
R5609 T21357 T21358 det The,issue
R5610 T21510 T21508 nsubj s,contain
R5611 T21511 T21510 punct -,s
R5612 T21512 T21508 aux does,contain
R5613 T21513 T21508 neg not,contain
R5614 T21358 T21359 nsubj issue,awaits
R5615 T21515 T21516 amod obvious,motifs
R5616 T21516 T21508 dobj motifs,contain
R5617 T21360 T21358 prep of,issue
R5618 T21517 T21518 npadvmod DNA,binding
R5619 T21518 T21516 amod binding,motifs
R5620 T21519 T21514 punct ", ",suppose
R5621 T21361 T21362 mark whether,forms
R5622 T21520 T21514 nsubj we,suppose
R5623 T21521 T21522 mark that,is
R5624 T21522 T21514 ccomp is,suppose
R5625 T21362 T21360 pcomp forms,of
R5626 T21523 T21522 expl there,is
R5627 T21524 T21525 det a,factor
R5628 T21525 T21522 attr factor,is
R5629 T21363 T21362 cc or,forms
R5630 T21526 T21527 npadvmod sequence,specific
R5631 T21527 T21525 amod specific,factor
R5632 T21528 T21527 punct -,specific
R5633 T21364 T21362 neg not,forms
R5634 T21529 T21525 compound transcription,factor
R5635 T21530 T21525 punct (,factor
R5636 T21531 T21525 nmod s,factor
R5637 T21365 T21366 compound mr,s
R5638 T21532 T21525 punct ),factor
R5639 T21533 T21534 dep which,interacts
R5640 T21534 T21525 relcl interacts,factor
R5641 T21366 T21362 nsubj s,forms
R5642 T21535 T21534 prep with,interacts
R5643 T21536 T21537 compound mr,s
R5644 T21537 T21535 pobj s,with
R5645 T21367 T21366 punct -,s
R5646 T21538 T21537 punct -,s
R5647 T21539 T21514 punct .,suppose
R5648 T21368 T21369 det a,polymer
R5649 T21541 T21542 advmod However,find
R5650 T21369 T21362 dobj polymer,forms
R5651 T21543 T21542 punct ", ",find
R5652 T21544 T21542 nsubj we,find
R5653 T21545 T21542 aux did,find
R5654 T21370 T21371 amod future,analysis
R5655 T21546 T21542 neg not,find
R5656 T21547 T21548 det any,factors
R5657 T21548 T21542 dobj factors,find
R5658 T21371 T21359 dobj analysis,awaits
R5659 T21549 T21548 compound transcription,factors
R5660 T21550 T21542 prep in,find
R5661 T21372 T21359 punct .,awaits
R5662 T21551 T21552 det the,screening
R5663 T21552 T21550 pobj screening,in
R5664 T21374 T21375 det A,report
R5665 T21553 T21552 amod present,screening
R5666 T21554 T21552 nmod yeast,screening
R5667 T21555 T21556 nummod two,hybrid
R5668 T21556 T21552 compound hybrid,screening
R5669 T21375 T21377 nsubj report,indicated
R5670 T21557 T21556 punct -,hybrid
R5671 T21558 T21542 punct .,find
R5672 T21376 T21375 amod previous,report
R5673 T21560 T21561 nsubj We,found
R5674 T21562 T21561 advmod also,found
R5675 T21378 T21379 mark that,contains
R5676 T21563 T21564 mark that,functions
R5677 T21564 T21561 ccomp functions,found
R5678 T21565 T21566 amod full,length
R5679 T21566 T21568 compound length,s
R5680 T21379 T21377 ccomp contains,indicated
R5681 T21567 T21566 punct -,length
R5682 T21568 T21564 nsubj s,functions
R5683 T21569 T21568 compound mr,s
R5684 T21380 T21379 nsubj TEL,contains
R5685 T21570 T21568 punct -,s
R5686 T21571 T21568 acl fused,s
R5687 T21572 T21571 prep to,fused
R5688 T21381 T21382 det a,domain
R5689 T21573 T21574 det the,domain
R5690 T21574 T21572 pobj domain,to
R5691 T21575 T21574 nmod GAL4,domain
R5692 T21382 T21379 dobj domain,contains
R5693 T21576 T21577 npadvmod DNA,binding
R5694 T21577 T21574 amod binding,domain
R5695 T21578 T21579 punct (,mrs
R5696 T21383 T21384 npadvmod sequence,specific
R5697 T21579 T21568 parataxis mrs,s
R5698 T21580 T21579 compound DBD,mrs
R5699 T21581 T21579 punct -,mrs
R5700 T21384 T21382 amod specific,domain
R5701 T21582 T21579 punct ),mrs
R5702 T21583 T21564 prep as,functions
R5703 T21584 T21585 det a,repressor
R5704 T21585 T21583 pobj repressor,as
R5705 T21586 T21585 amod transcriptional,repressor
R5706 T21587 T21561 punct .,found
R5707 T21595 T21593 acl involved,idea
R5708 T21589 T21590 nsubj This,support
R5709 T21591 T21590 aux may,support
R5710 T21592 T21593 det the,idea
R5711 T21596 T21597 compound mr,s
R5712 T21593 T21590 dobj idea,support
R5713 T21594 T21595 mark that,involved
R5714 T21597 T21595 nsubjpass s,involved
R5715 T21598 T21597 punct -,s
R5716 T21599 T21595 auxpass is,involved
R5717 T21600 T21595 prep in,involved
R5718 T21700 T21695 prep at,contains
R5719 T21701 T21702 det the,terminus
R5720 T21601 T21602 amod repressive,complexes
R5721 T21702 T21700 pobj terminus,at
R5722 T21703 T21702 compound C,terminus
R5723 T21602 T21600 pobj complexes,in
R5724 T21704 T21702 punct -,terminus
R5725 T21705 T21670 punct ", ",reported
R5726 T21706 T21670 auxpass was,reported
R5727 T21603 T21602 amod similar,complexes
R5728 T21707 T21670 prep as,reported
R5729 T21708 T21709 det a,repressor
R5730 T21604 T21603 prep to,similar
R5731 T21709 T21707 pobj repressor,as
R5732 T21710 T21709 amod transcriptional,repressor
R5733 T21711 T21670 cc and,reported
R5734 T21605 T21606 amod other,proteins
R5735 T21712 T21713 det the,activity
R5736 T21606 T21604 pobj proteins,to
R5737 T21713 T21715 nsubj activity,required
R5738 T21607 T21608 compound SAM,domain
R5739 T21714 T21713 compound repressor,activity
R5740 T21715 T21670 conj required,reported
R5741 T21608 T21609 npadvmod domain,containing
R5742 T21716 T21713 prep of,activity
R5743 T21717 T21718 nmod H,MBT
R5744 T21609 T21606 amod containing,proteins
R5745 T21718 T21716 pobj MBT,of
R5746 T21719 T21720 punct -,L
R5747 T21720 T21717 prep L,H
R5748 T21610 T21609 punct -,containing
R5749 T21721 T21722 punct (,3
R5750 T21722 T21717 parataxis 3,H
R5751 T21723 T21722 punct ),3
R5752 T21611 T21590 punct .,support
R5753 T21724 T21715 advmod mainly,required
R5754 T21725 T21726 det the,presence
R5755 T21726 T21715 dobj presence,required
R5756 T21727 T21726 prep of,presence
R5757 T21728 T21729 det the,repeats
R5758 T21613 T21614 poss Our,results
R5759 T21729 T21727 pobj repeats,of
R5760 T21730 T21729 compound MBT,repeats
R5761 T21731 T21729 cc but,repeats
R5762 T21732 T21731 neg not,but
R5763 T21733 T21734 det the,domain
R5764 T21614 T21615 nsubj results,showed
R5765 T21734 T21729 conj domain,repeats
R5766 T21735 T21734 compound SAM,domain
R5767 T21616 T21615 punct ", ",showed
R5768 T21736 T21737 punct [,45
R5769 T21737 T21715 parataxis 45,required
R5770 T21738 T21737 punct ],45
R5771 T21739 T21670 punct .,reported
R5772 T21617 T21615 advmod however,showed
R5773 T21741 T21742 aux To,determine
R5774 T21618 T21615 punct ", ",showed
R5775 T21742 T21743 advcl determine,performed
R5776 T21744 T21745 det the,region
R5777 T21619 T21620 mark that,is
R5778 T21745 T21742 dobj region,determine
R5779 T21746 T21745 amod transcriptional,region
R5780 T21747 T21745 compound repressor,region
R5781 T21620 T21615 ccomp is,showed
R5782 T21748 T21745 prep of,region
R5783 T21749 T21750 det the,protein
R5784 T21750 T21748 pobj protein,of
R5785 T21621 T21622 det the,association
R5786 T21751 T21752 compound mr,s
R5787 T21752 T21750 compound s,protein
R5788 T21753 T21752 punct -,s
R5789 T21622 T21620 nsubj association,is
R5790 T21754 T21743 punct ", ",performed
R5791 T21755 T21743 nsubj we,performed
R5792 T21756 T21757 det a,assay
R5793 T21623 T21622 compound self,association
R5794 T21757 T21743 dobj assay,performed
R5795 T21758 T21757 compound luciferase,assay
R5796 T21759 T21743 advcl using,performed
R5797 T21624 T21622 punct -,association
R5798 T21760 T21761 npadvmod site,directed
R5799 T21761 T21763 amod directed,mutants
R5800 T21762 T21761 punct -,directed
R5801 T21625 T21622 prep of,association
R5802 T21763 T21759 dobj mutants,using
R5803 T21764 T21765 punct (,W404A
R5804 T21765 T21743 parataxis W404A,performed
R5805 T21626 T21627 compound mr,s
R5806 T21766 T21765 compound DBD,W404A
R5807 T21767 T21765 punct -,W404A
R5808 T21768 T21765 cc and,W404A
R5809 T21769 T21770 compound DBD,G453A
R5810 T21770 T21765 conj G453A,W404A
R5811 T21771 T21770 punct -,G453A
R5812 T21772 T21765 punct ),W404A
R5813 T21627 T21625 pobj s,of
R5814 T21773 T21743 punct .,performed
R5815 T21775 T21776 det The,result
R5816 T21628 T21627 punct -,s
R5817 T21776 T21777 nsubj result,showed
R5818 T21778 T21779 mark that,compromises
R5819 T21629 T21622 prep through,association
R5820 T21779 T21777 ccomp compromises,showed
R5821 T21780 T21781 det the,ability
R5822 T21781 T21779 nsubj ability,compromises
R5823 T21630 T21631 poss its,domain
R5824 T21782 T21781 amod reduced,ability
R5825 T21783 T21781 compound binding,ability
R5826 T21631 T21629 pobj domain,through
R5827 T21784 T21781 prep of,ability
R5828 T21785 T21786 compound self,association
R5829 T21786 T21784 pobj association,of
R5830 T21632 T21631 compound SAM,domain
R5831 T21787 T21786 punct -,association
R5832 T21788 T21779 advmod partially,compromises
R5833 T21789 T21790 det the,activity
R5834 T21633 T21620 neg not,is
R5835 T21790 T21779 dobj activity,compromises
R5836 T21791 T21790 amod transcriptional,activity
R5837 T21634 T21620 acomp essential,is
R5838 T21792 T21790 amod repressive,activity
R5839 T21793 T21790 prep of,activity
R5840 T21794 T21795 compound mr,s
R5841 T21635 T21634 prep for,essential
R5842 T21795 T21793 pobj s,of
R5843 T21796 T21795 punct -,s
R5844 T21797 T21798 punct (,7D
R5845 T21636 T21637 det the,activity
R5846 T21637 T21635 pobj activity,for
R5847 T21798 T21779 parataxis 7D,compromises
R5848 T21638 T21637 amod transcriptional,activity
R5849 T21799 T21798 compound Fig.,7D
R5850 T21800 T21798 punct ),7D
R5851 T21801 T21777 punct .,showed
R5852 T21639 T21637 amod repressive,activity
R5853 T21640 T21615 punct .,showed
R5854 T21803 T21804 advmod However,was
R5855 T21642 T21643 nsubjpass Polymerization,reported
R5856 T21644 T21642 prep of,Polymerization
R5857 T21805 T21804 punct ", ",was
R5858 T21806 T21807 det the,effect
R5859 T21807 T21804 nsubj effect,was
R5860 T21808 T21807 amod repressive,effect
R5861 T21809 T21810 advmod more,significant
R5862 T21810 T21804 acomp significant,was
R5863 T21645 T21646 det the,domain
R5864 T21811 T21812 advmod when,co-introduced
R5865 T21812 T21804 advcl co-introduced,was
R5866 T21813 T21814 compound DBD,tail
R5867 T21646 T21644 pobj domain,of
R5868 T21647 T21646 compound SAM,domain
R5869 T21648 T21643 aux has,reported
R5870 T21814 T21812 nsubjpass tail,co-introduced
R5871 T21649 T21643 auxpass been,reported
R5872 T21815 T21814 punct -,tail
R5873 T21816 T21814 punct ", ",tail
R5874 T21817 T21818 dep which,contain
R5875 T21650 T21643 advmod previously,reported
R5876 T21818 T21814 relcl contain,tail
R5877 T21819 T21818 aux does,contain
R5878 T21651 T21652 aux to,be
R5879 T21820 T21818 neg not,contain
R5880 T21821 T21822 compound SAM,domain
R5881 T21822 T21818 dobj domain,contain
R5882 T21652 T21643 xcomp be,reported
R5883 T21823 T21812 punct ", ",co-introduced
R5884 T21824 T21812 auxpass was,co-introduced
R5885 T21825 T21812 prep with,co-introduced
R5886 T21653 T21652 acomp essential,be
R5887 T21826 T21827 det the,plasmid
R5888 T21827 T21825 pobj plasmid,with
R5889 T21654 T21653 prep for,essential
R5890 T21828 T21829 compound 5xGAL4,pGL3
R5891 T21829 T21827 compound pGL3,plasmid
R5892 T21830 T21829 punct -,pGL3
R5893 T21655 T21656 det the,functions
R5894 T21831 T21827 compound reporter,plasmid
R5895 T21832 T21804 punct .,was
R5896 T21656 T21654 pobj functions,for
R5897 T21834 T21835 advmod Therefore,conclude
R5898 T21657 T21656 amod repressive,functions
R5899 T21836 T21835 punct ", ",conclude
R5900 T21837 T21835 nsubj we,conclude
R5901 T21838 T21839 mark that,is
R5902 T21658 T21656 prep of,functions
R5903 T21839 T21835 ccomp is,conclude
R5904 T21840 T21841 det a,region
R5905 T21659 T21658 pobj ph,of
R5906 T21841 T21839 nsubj region,is
R5907 T21842 T21841 prep from,region
R5908 T21843 T21844 nmod amino,463
R5909 T21660 T21659 cc and,ph
R5910 T21844 T21842 pobj 463,from
R5911 T21845 T21844 nmod acids,463
R5912 T21846 T21844 prep to,463
R5913 T21847 T21846 pobj 542,to
R5914 T21848 T21849 advmod mainly,responsible
R5915 T21849 T21839 acomp responsible,is
R5916 T21661 T21659 conj TEL,ph
R5917 T21850 T21849 prep for,responsible
R5918 T21851 T21852 det the,activity
R5919 T21852 T21850 pobj activity,for
R5920 T21662 T21663 punct [,44
R5921 T21853 T21852 compound repressor,activity
R5922 T21854 T21839 prep in,is
R5923 T21855 T21856 det the,case
R5924 T21663 T21643 parataxis 44,reported
R5925 T21856 T21854 pobj case,in
R5926 T21857 T21856 prep of,case
R5927 T21664 T21663 nummod 43,44
R5928 T21858 T21859 compound mr,s
R5929 T21859 T21857 pobj s,of
R5930 T21860 T21859 punct -,s
R5931 T21665 T21663 punct ",",44
R5932 T21861 T21835 punct .,conclude
R5933 T21666 T21663 punct ],44
R5934 T21863 T21864 amod Evolutionary,conservation
R5935 T21864 T21865 nsubj conservation,underlie
R5936 T21667 T21643 punct .,reported
R5937 T21866 T21864 prep of,conservation
R5938 T21867 T21868 det the,region
R5939 T21868 T21866 pobj region,of
R5940 T21669 T21670 prep On,reported
R5941 T21869 T21870 npadvmod C,terminal
R5942 T21870 T21868 amod terminal,region
R5943 T21871 T21870 punct -,terminal
R5944 T21671 T21672 det the,hand
R5945 T21872 T21873 nummod 80,aa
R5946 T21873 T21868 compound aa,region
R5947 T21874 T21868 prep of,region
R5948 T21875 T21876 compound mr,s
R5949 T21672 T21669 pobj hand,On
R5950 T21876 T21874 pobj s,of
R5951 T21877 T21876 punct -,s
R5952 T21673 T21672 amod other,hand
R5953 T21878 T21864 prep from,conservation
R5954 T21879 T21878 pobj zebrafish,from
R5955 T21880 T21878 prep through,from
R5956 T21674 T21670 punct ", ",reported
R5957 T21881 T21880 pobj human,through
R5958 T21882 T21865 aux may,underlie
R5959 T21883 T21884 det the,importance
R5960 T21675 T21676 amod human,protein
R5961 T21884 T21865 dobj importance,underlie
R5962 T21885 T21884 amod functional,importance
R5963 T21676 T21670 nsubjpass protein,reported
R5964 T21886 T21884 prep of,importance
R5965 T21887 T21888 det this,region
R5966 T21888 T21886 pobj region,of
R5967 T21677 T21676 amod lethal,protein
R5968 T21889 T21865 punct .,underlie
R5969 T21891 T21892 nummod Two,complexes
R5970 T21678 T21679 punct (,3
R5971 T21892 T21896 nsubjpass complexes,identified
R5972 T21893 T21892 amod distinct,complexes
R5973 T21679 T21677 parataxis 3,lethal
R5974 T21894 T21892 compound multiprotein,complexes
R5975 T21895 T21892 compound PcG,complexes
R5976 T21680 T21679 punct ),3
R5977 T21897 T21892 punct ", ",complexes
R5978 T21898 T21892 appos PRC1,complexes
R5979 T21681 T21682 amod malignant,tumor
R5980 T21899 T21898 cc and,PRC1
R5981 T21900 T21898 conj PRC2,PRC1
R5982 T21901 T21896 punct ", ",identified
R5983 T21682 T21676 nmod tumor,protein
R5984 T21902 T21896 aux have,identified
R5985 T21903 T21896 auxpass been,identified
R5986 T21683 T21682 nmod brain,tumor
R5987 T21904 T21896 punct .,identified
R5988 T21906 T21907 nsubjpass PRC2,involved
R5989 T21684 T21685 punct (,3
R5990 T21908 T21907 auxpass is,involved
R5991 T21909 T21907 prep in,involved
R5992 T21685 T21682 parataxis 3,tumor
R5993 T21910 T21911 det the,initiation
R5994 T21686 T21685 dep H,3
R5995 T21687 T21688 punct -,L
R5996 T21688 T21686 prep L,H
R5997 T21911 T21909 pobj initiation,in
R5998 T21912 T21911 prep of,initiation
R5999 T21913 T21912 pobj silencing,of
R6000 T21689 T21685 punct (,3
R6001 T21914 T21907 cc and,involved
R6002 T21915 T21907 conj contains,involved
R6003 T21690 T21685 punct ),3
R6004 T21916 T21917 compound histone,deacetylases
R6005 T21917 T21915 dobj deacetylases,contains
R6006 T21918 T21917 punct (,deacetylases
R6007 T21691 T21682 appos MBT,tumor
R6008 T21919 T21917 appos HDACs,deacetylases
R6009 T21692 T21676 punct ),protein
R6010 T21920 T21917 punct ),deacetylases
R6011 T21921 T21917 cc and,deacetylases
R6012 T21922 T21923 compound histone,methyltransferases
R6013 T21923 T21917 conj methyltransferases,deacetylases
R6014 T21693 T21676 punct ", ",protein
R6015 T21924 T21917 punct ", ",deacetylases
R6016 T21925 T21926 dep which,methylate
R6017 T21926 T21917 relcl methylate,deacetylases
R6018 T21927 T21926 aux can,methylate
R6019 T21928 T21929 nmod histone,9
R6020 T21929 T21926 dobj 9,methylate
R6021 T21694 T21695 dep which,contains
R6022 T21930 T21929 nmod H3,9
R6023 T21931 T21929 nmod lysine,9
R6024 T21932 T21929 cc and,9
R6025 T21695 T21676 relcl contains,protein
R6026 T21933 T21929 conj 27,9
R6027 T21934 T21929 punct ", ",9
R6028 T21935 T21929 appos marks,9
R6029 T21696 T21695 advmod also,contains
R6030 T21936 T21935 prep of,marks
R6031 T21937 T21938 amod silenced,chromatin
R6032 T21938 T21936 pobj chromatin,of
R6033 T21697 T21698 det a,domain
R6034 T21939 T21907 punct .,involved
R6035 T21941 T21942 nsubj PRC1,recognizes
R6036 T21698 T21695 dobj domain,contains
R6037 T21943 T21941 punct ", ",PRC1
R6038 T21699 T21698 compound SAM,domain
R6039 T21944 T21941 prep including,PRC1
R6040 T21945 T21944 pobj ph,including
R6041 T21946 T21942 punct ", ",recognizes
R6042 T22017 T22018 poss Our,assay
R6043 T21947 T21948 det the,mark
R6044 T21948 T21942 dobj mark,recognizes
R6045 T21949 T21948 nmod histone,mark
R6046 T21950 T21948 nmod H3,mark
R6047 T22018 T22020 nsubj assay,showed
R6048 T21951 T21948 nmod lysine,mark
R6049 T21952 T21951 nummod 27,lysine
R6050 T21953 T21948 acl set,mark
R6051 T22019 T22018 compound luciferase,assay
R6052 T21954 T21953 agent by,set
R6053 T21955 T21954 pobj PRC2,by
R6054 T22021 T22020 advmod also,showed
R6055 T21956 T21942 cc and,recognizes
R6056 T21957 T21942 conj maintains,recognizes
R6057 T21958 T21959 det a,state
R6058 T21959 T21957 dobj state,maintains
R6059 T22022 T22023 mark that,affected
R6060 T21960 T21959 amod stable,state
R6061 T22023 T22020 ccomp affected,showed
R6062 T21961 T21959 prep of,state
R6063 T21962 T21963 compound gene,repression
R6064 T21963 T21961 pobj repression,of
R6065 T21964 T21965 prep in,blocks
R6066 T22024 T22025 amod transcriptional,repression
R6067 T21965 T21959 relcl blocks,state
R6068 T21966 T21964 pobj which,in
R6069 T21967 T21965 nsubj PRC1,blocks
R6070 T21968 T21969 compound chromatin,remodeling
R6071 T21969 T21965 dobj remodeling,blocks
R6072 T21970 T21969 prep by,remodeling
R6073 T22025 T22023 nsubjpass repression,affected
R6074 T21971 T21972 det the,complex
R6075 T22026 T22025 prep of,repression
R6076 T21972 T21970 pobj complex,by
R6077 T22027 T22028 compound DBD,mrs
R6078 T21973 T21972 nmod trithorax,complex
R6079 T21974 T21975 npadvmod group,related
R6080 T22028 T22026 pobj mrs,of
R6081 T21975 T21972 amod related,complex
R6082 T21976 T21975 punct -,related
R6083 T21977 T21978 compound SWI,SNF
R6084 T22029 T22028 punct -,mrs
R6085 T21978 T21972 compound SNF,complex
R6086 T21979 T21978 punct -,SNF
R6087 T22030 T22023 auxpass was,affected
R6088 T21980 T21981 punct [,47
R6089 T21981 T21965 parataxis 47,blocks
R6090 T22031 T22023 neg not,affected
R6091 T21982 T21981 nummod 46,47
R6092 T21983 T21981 punct ",",47
R6093 T21984 T21981 punct ],47
R6094 T22032 T22023 agent by,affected
R6095 T21985 T21942 punct .,recognizes
R6096 T22033 T22034 det the,addition
R6097 T21987 T21988 advmod Therefore,thought
R6098 T21989 T21988 punct ", ",thought
R6099 T22034 T22032 pobj addition,by
R6100 T21990 T21991 det the,mechanism
R6101 T21991 T21988 nsubjpass mechanism,thought
R6102 T21992 T21991 prep of,mechanism
R6103 T22035 T22034 prep of,addition
R6104 T21993 T21992 pobj repression,of
R6105 T21994 T21993 prep by,repression
R6106 T21995 T21994 pobj PRC2,by
R6107 T22036 T22037 amod various,concentrations
R6108 T21996 T21988 auxpass is,thought
R6109 T21997 T21998 aux to,be
R6110 T22037 T22035 pobj concentrations,of
R6111 T21998 T21988 xcomp be,thought
R6112 T21999 T22000 npadvmod HDAC,dependent
R6113 T22000 T21998 acomp dependent,be
R6114 T22038 T22037 prep of,concentrations
R6115 T22001 T22000 punct -,dependent
R6116 T22039 T22040 compound trichostatin,A
R6117 T22002 T22003 mark while,appears
R6118 T22003 T21988 advcl appears,thought
R6119 T22004 T22005 det the,mechanism
R6120 T22005 T22003 nsubj mechanism,appears
R6121 T22006 T22005 prep of,mechanism
R6122 T22007 T22006 pobj repression,of
R6123 T22008 T22007 prep by,repression
R6124 T22040 T22038 pobj A,of
R6125 T22009 T22008 pobj PRC1,by
R6126 T22010 T22011 aux to,be
R6127 T22011 T22003 xcomp be,appears
R6128 T22041 T22040 punct ", ",A
R6129 T22012 T22013 npadvmod HDAC,independent
R6130 T22013 T22011 acomp independent,be
R6131 T22014 T22013 punct -,independent
R6132 T22042 T22043 det a,inhibitor
R6133 T22015 T21988 punct .,thought
R6134 T22043 T22040 appos inhibitor,A
R6135 T22044 T22043 amod potent,inhibitor
R6136 T22045 T22043 compound HDAC,inhibitor
R6137 T22046 T22047 punct (,shown
R6138 T22124 T22123 advmod also,revealed
R6139 T22125 T22126 mark that,is
R6140 T22047 T22020 parataxis shown,showed
R6141 T22126 T22123 ccomp is,revealed
R6142 T22127 T22128 det the,activity
R6143 T22128 T22126 nsubj activity,is
R6144 T22048 T22047 nsubj data,shown
R6145 T22129 T22128 amod repressive,activity
R6146 T22130 T22128 prep of,activity
R6147 T22049 T22047 neg not,shown
R6148 T22131 T22132 compound mr,s
R6149 T22132 T22130 pobj s,of
R6150 T22133 T22132 punct -,s
R6151 T22050 T22047 punct ),shown
R6152 T22134 T22135 advmod mainly,due
R6153 T22135 T22126 prep due,is
R6154 T22136 T22135 pcomp to,due
R6155 T22051 T22020 punct .,showed
R6156 T22137 T22138 det the,region
R6157 T22138 T22135 pobj region,due
R6158 T22139 T22140 npadvmod C,terminal
R6159 T22053 T22054 advmod Therefore,speculate
R6160 T22140 T22138 amod terminal,region
R6161 T22141 T22140 punct -,terminal
R6162 T22142 T22143 punct (,463
R6163 T22055 T22054 punct ", ",speculate
R6164 T22143 T22138 parataxis 463,region
R6165 T22056 T22054 nsubj we,speculate
R6166 T22144 T22143 nmod amino,463
R6167 T22145 T22143 nmod acids,463
R6168 T22057 T22058 mark that,be
R6169 T22146 T22143 prep to,463
R6170 T22147 T22146 pobj 542,to
R6171 T22148 T22143 punct ),463
R6172 T22149 T22123 punct .,revealed
R6173 T22151 T22152 advmod However,remain
R6174 T22058 T22054 ccomp be,speculate
R6175 T22153 T22152 punct ", ",remain
R6176 T22154 T22155 amod downstream,targets
R6177 T22059 T22060 det the,mechanism
R6178 T22155 T22152 nsubj targets,remain
R6179 T22156 T22155 prep of,targets
R6180 T22060 T22058 nsubj mechanism,be
R6181 T22157 T22158 compound mr,s
R6182 T22158 T22156 pobj s,of
R6183 T22061 T22060 prep of,mechanism
R6184 T22159 T22158 punct -,s
R6185 T22160 T22152 advmod still,remain
R6186 T22161 T22152 acomp unclear,remain
R6187 T22062 T22061 pobj repression,of
R6188 T22162 T22152 punct .,remain
R6189 T22164 T22165 advmod In,situ
R6190 T22063 T22062 prep of,repression
R6191 T22165 T22166 amod situ,hybridization
R6192 T22166 T22167 nsubj hybridization,showed
R6193 T22064 T22065 compound mr,s
R6194 T22168 T22169 mark that,is
R6195 T22169 T22167 ccomp is,showed
R6196 T22065 T22063 pobj s,of
R6197 T22170 T22171 det the,peak
R6198 T22171 T22169 nsubj peak,is
R6199 T22172 T22171 prep of,peak
R6200 T22066 T22065 punct -,s
R6201 T22173 T22174 compound mr,s
R6202 T22174 T22176 compound s,expression
R6203 T22175 T22174 punct -,s
R6204 T22067 T22058 aux may,be
R6205 T22176 T22172 pobj expression,of
R6206 T22177 T22178 advmod around,P6
R6207 T22178 T22169 attr P6,is
R6208 T22068 T22069 npadvmod HDAC,independent
R6209 T22179 T22178 punct ", ",P6
R6210 T22180 T22181 advmod when,undergo
R6211 T22069 T22058 acomp independent,be
R6212 T22181 T22178 relcl undergo,P6
R6213 T22182 T22183 amod retinal,photoreceptors
R6214 T22183 T22181 nsubj photoreceptors,undergo
R6215 T22070 T22069 punct -,independent
R6216 T22184 T22185 amod terminal,differentiation
R6217 T22185 T22181 dobj differentiation,undergo
R6218 T22186 T22167 punct .,showed
R6219 T22071 T22069 cc and,independent
R6220 T22188 T22189 nsubj We,hypothesize
R6221 T22072 T22073 advmod more,similar
R6222 T22190 T22191 mark that,be
R6223 T22191 T22189 ccomp be,hypothesize
R6224 T22192 T22193 det the,genes
R6225 T22193 T22191 nsubj genes,be
R6226 T22194 T22193 compound target,genes
R6227 T22195 T22193 prep of,genes
R6228 T22073 T22069 conj similar,independent
R6229 T22196 T22197 compound mr,s
R6230 T22197 T22195 pobj s,of
R6231 T22198 T22197 punct -,s
R6232 T22074 T22073 prep to,similar
R6233 T22199 T22191 aux might,be
R6234 T22200 T22201 amod non-photoreceptor,genes
R6235 T22201 T22191 attr genes,be
R6236 T22075 T22074 pobj that,to
R6237 T22202 T22189 punct .,hypothesize
R6238 T22076 T22075 prep of,that
R6239 T22204 T22205 prep In,suppress
R6240 T22206 T22207 det this,case
R6241 T22077 T22078 compound PRC1,complex
R6242 T22207 T22204 pobj case,In
R6243 T22078 T22076 pobj complex,of
R6244 T22079 T22054 punct .,speculate
R6245 T22081 T22082 prep In,demonstrate
R6246 T22208 T22205 punct ", ",suppress
R6247 T22209 T22210 compound mr,s
R6248 T22083 T22084 det the,study
R6249 T22210 T22205 nsubj s,suppress
R6250 T22211 T22210 punct -,s
R6251 T22212 T22205 aux may,suppress
R6252 T22213 T22214 det the,expression
R6253 T22084 T22081 pobj study,In
R6254 T22214 T22205 dobj expression,suppress
R6255 T22215 T22214 prep of,expression
R6256 T22085 T22084 amod present,study
R6257 T22216 T22217 amod non-photoreceptor,genes
R6258 T22217 T22215 pobj genes,of
R6259 T22218 T22205 prep in,suppress
R6260 T22086 T22082 punct ", ",demonstrate
R6261 T22219 T22220 nmod rod,photoreceptors
R6262 T22220 T22218 pobj photoreceptors,in
R6263 T22221 T22219 cc and,rod
R6264 T22087 T22088 det the,experiments
R6265 T22222 T22219 conj cone,rod
R6266 T22223 T22205 punct .,suppress
R6267 T22088 T22082 nsubj experiments,demonstrate
R6268 T22225 T22226 expl There,be
R6269 T22227 T22226 aux may,be
R6270 T22089 T22088 amod biochemical,experiments
R6271 T22228 T22229 det another,possibility
R6272 T22090 T22091 mark that,functions
R6273 T22091 T22082 ccomp functions,demonstrate
R6274 T22092 T22093 det the,protein
R6275 T22229 T22226 attr possibility,be
R6276 T22230 T22231 mark that,involved
R6277 T22231 T22229 acl involved,possibility
R6278 T22232 T22233 compound mr,s
R6279 T22233 T22231 nsubjpass s,involved
R6280 T22093 T22091 nsubj protein,functions
R6281 T22234 T22233 punct -,s
R6282 T22235 T22231 auxpass is,involved
R6283 T22094 T22095 compound mr,s
R6284 T22095 T22093 compound s,protein
R6285 T22236 T22231 prep in,involved
R6286 T22237 T22238 compound cell,fate
R6287 T22238 T22239 compound fate,determination
R6288 T22096 T22095 punct -,s
R6289 T22239 T22236 pobj determination,in
R6290 T22240 T22239 prep of,determination
R6291 T22097 T22091 prep as,functions
R6292 T22241 T22242 compound rod,photoreceptors
R6293 T22242 T22240 pobj photoreceptors,of
R6294 T22243 T22242 cc versus,photoreceptors
R6295 T22098 T22099 det a,repressor
R6296 T22244 T22245 compound cone,photoreceptors
R6297 T22245 T22242 conj photoreceptors,photoreceptors
R6298 T22099 T22097 pobj repressor,as
R6299 T22246 T22226 punct .,be
R6300 T22248 T22249 mark While,born
R6301 T22100 T22099 amod transcriptional,repressor
R6302 T22249 T22253 advcl born,born
R6303 T22250 T22251 compound cone,photoreceptors
R6304 T22101 T22091 cc and,functions
R6305 T22251 T22249 nsubjpass photoreceptors,born
R6306 T22252 T22249 auxpass are,born
R6307 T22102 T22103 advmod possibly,regulates
R6308 T22254 T22249 prep during,born
R6309 T22255 T22256 det the,stages
R6310 T22256 T22254 pobj stages,during
R6311 T22103 T22091 conj regulates,functions
R6312 T22257 T22258 amod early,embryonic
R6313 T22258 T22256 amod embryonic,stages
R6314 T22104 T22103 advmod down,regulates
R6315 T22259 T22256 prep of,stages
R6316 T22260 T22261 compound mouse,retinogenesis
R6317 T22261 T22259 pobj retinogenesis,of
R6318 T22105 T22103 punct -,regulates
R6319 T22262 T22253 punct ", ",born
R6320 T22263 T22264 compound rod,photoreceptors
R6321 T22106 T22107 det the,expression
R6322 T22264 T22253 nsubjpass photoreceptors,born
R6323 T22265 T22253 auxpass are,born
R6324 T22266 T22253 advmod primarily,born
R6325 T22107 T22103 dobj expression,regulates
R6326 T22267 T22253 prep in,born
R6327 T22268 T22269 det the,period
R6328 T22269 T22267 pobj period,in
R6329 T22270 T22271 amod late,embryonic
R6330 T22271 T22269 amod embryonic,period
R6331 T22108 T22107 amod spatial,expression
R6332 T22272 T22271 cc and,embryonic
R6333 T22273 T22274 amod early,postnatal
R6334 T22274 T22271 conj postnatal,embryonic
R6335 T22275 T22276 punct [,48
R6336 T22109 T22108 cc and,spatial
R6337 T22276 T22253 parataxis 48,born
R6338 T22277 T22276 punct ],48
R6339 T22110 T22108 conj temporal,spatial
R6340 T22278 T22253 punct .,born
R6341 T22280 T22281 det The,pattern
R6342 T22111 T22107 prep of,expression
R6343 T22281 T22283 nsubj pattern,suggest
R6344 T22282 T22281 compound expression,pattern
R6345 T22112 T22113 det the,genes
R6346 T22284 T22281 prep of,pattern
R6347 T22285 T22286 compound mr,s
R6348 T22113 T22111 pobj genes,of
R6349 T22286 T22284 pobj s,of
R6350 T22287 T22286 punct -,s
R6351 T22288 T22283 aux may,suggest
R6352 T22114 T22113 compound target,genes
R6353 T22289 T22290 mark that,expressed
R6354 T22290 T22283 ccomp expressed,suggest
R6355 T22291 T22292 compound mr,s
R6356 T22115 T22103 prep during,regulates
R6357 T22292 T22290 nsubjpass s,expressed
R6358 T22293 T22292 punct -,s
R6359 T22116 T22117 amod retinal,development
R6360 T22294 T22290 auxpass is,expressed
R6361 T22295 T22290 prep in,expressed
R6362 T22296 T22297 compound rod,photoreceptors
R6363 T22117 T22115 pobj development,during
R6364 T22297 T22295 pobj photoreceptors,in
R6365 T22298 T22295 cc but,in
R6366 T22299 T22298 neg not,but
R6367 T22118 T22117 compound photoreceptor,development
R6368 T22300 T22295 conj in,in
R6369 T22301 T22302 compound cone,photoreceptors
R6370 T22302 T22300 pobj photoreceptors,in
R6371 T22303 T22304 advmod as,as
R6372 T22119 T22082 punct .,demonstrate
R6373 T22304 T22302 cc as,photoreceptors
R6374 T22305 T22304 advmod well,as
R6375 T22306 T22302 conj Nr2e3,photoreceptors
R6376 T22121 T22122 poss Our,data
R6377 T22307 T22306 punct ", ",Nr2e3
R6378 T22308 T22309 dep which,known
R6379 T22309 T22306 relcl known,Nr2e3
R6380 T22310 T22309 auxpass is,known
R6381 T22311 T22309 prep as,known
R6382 T22312 T22313 det a,repressor
R6383 T22313 T22311 pobj repressor,as
R6384 T22122 T22123 nsubj data,revealed
R6385 T22314 T22313 amod transcriptional,repressor
R6386 T22315 T22309 cc and,known
R6387 T22316 T22317 auxpass is,thought
R6389 T22317 T22309 conj thought,known
R6390 T22318 T22319 aux to,regulate
R6394 T22320 T22319 advmod down,regulate
R6396 T22321 T22319 punct -,regulate
R6427 T22676 T22677 advmod Here,identified
R6428 T22678 T22677 nsubj we,identified
R6429 T22679 T22680 compound mouse,s
R6430 T22680 T22677 dobj s,identified
R6431 T22681 T22680 compound mr,s
R6432 T22682 T22680 punct -,s
R6433 T22683 T22680 punct ", ",s
R6434 T22684 T22685 dep which,expressed
R6435 T22685 T22680 relcl expressed,s
R6436 T22686 T22685 auxpass is,expressed
R6437 T22687 T22685 advmod predominantly,expressed
R6438 T22688 T22685 prep in,expressed
R6439 T22689 T22690 amod retinal,photoreceptors
R6440 T22690 T22688 pobj photoreceptors,in
R6441 T22691 T22690 cc and,photoreceptors
R6442 T22692 T22693 det the,gland
R6443 T22693 T22690 conj gland,photoreceptors
R6444 T22694 T22693 amod pineal,gland
R6445 T22695 T22677 punct .,identified
R6446 T22697 T22698 compound mr,s
R6447 T22698 T22700 nsubjpass s,conserved
R6448 T22699 T22698 punct -,s
R6449 T22701 T22700 auxpass is,conserved
R6450 T22702 T22700 advmod evolutionarily,conserved
R6451 T22703 T22700 prep from,conserved
R6452 T22704 T22703 pobj zebrafish,from
R6453 T22705 T22703 prep through,from
R6454 T22706 T22705 prep to,through
R6455 T22707 T22706 pobj human,to
R6456 T22708 T22700 punct ", ",conserved
R6457 T22709 T22700 advcl suggesting,conserved
R6458 T22710 T22711 det a,role
R6459 T22711 T22709 dobj role,suggesting
R6460 T22712 T22711 amod significant,role
R6461 T22713 T22711 prep of,role
R6462 T22714 T22715 compound mr,s
R6463 T22715 T22713 pobj s,of
R6464 T22716 T22715 punct -,s
R6465 T22717 T22711 prep in,role
R6466 T22718 T22719 compound photoreceptor,development
R6467 T22719 T22717 pobj development,in
R6468 T22720 T22700 punct .,conserved
R6469 T22722 T22723 poss Our,data
R6470 T22723 T22725 nsubj data,suggest
R6471 T22724 T22723 amod present,data
R6472 T22726 T22727 mark that,localizes
R6473 T22727 T22725 ccomp localizes,suggest
R6474 T22728 T22729 compound mr,s
R6475 T22729 T22731 compound s,protein
R6476 T22730 T22729 punct -,s
R6477 T22731 T22727 nsubj protein,localizes
R6478 T22732 T22727 prep in,localizes
R6479 T22733 T22734 det the,nucleus
R6480 T22734 T22732 pobj nucleus,in
R6481 T22735 T22727 cc and,localizes
R6482 T22736 T22737 aux can,associated
R6483 T22737 T22727 conj associated,localizes
R6484 T22738 T22737 dep self,associated
R6485 T22739 T22737 punct -,associated
R6486 T22740 T22741 advmod mainly,through
R6487 T22741 T22737 prep through,associated
R6488 T22742 T22743 det the,domain
R6489 T22743 T22741 pobj domain,through
R6490 T22744 T22743 compound SAM,domain
R6491 T22745 T22725 punct .,suggest
R6492 T22747 T22748 advmod Moreover,functions
R6493 T22749 T22748 punct ", ",functions
R6494 T22750 T22751 compound mr,s
R6495 T22751 T22753 compound s,protein
R6496 T22752 T22751 punct -,s
R6497 T22753 T22748 nsubj protein,functions
R6498 T22754 T22753 acl fused,protein
R6499 T22755 T22754 prep to,fused
R6500 T22756 T22757 compound GAL4,DBD
R6501 T22757 T22755 pobj DBD,to
R6502 T22758 T22748 prep as,functions
R6503 T22759 T22760 det a,repressor
R6504 T22760 T22758 pobj repressor,as
R6505 T22761 T22760 amod transcriptional,repressor
R6506 T22762 T22748 punct .,functions
R6507 T22764 T22765 det The,activity
R6508 T22765 T22767 nsubj activity,is
R6509 T22766 T22765 amod repressive,activity
R6510 T22768 T22765 prep of,activity
R6511 T22769 T22770 compound mr,s
R6512 T22770 T22768 pobj s,of
R6513 T22771 T22770 punct -,s
R6514 T22772 T22767 prep due,is
R6515 T22773 T22772 pcomp to,due
R6516 T22774 T22775 poss its,region
R6517 T22775 T22772 pobj region,due
R6518 T22776 T22777 npadvmod C,terminal
R6519 T22777 T22775 amod terminal,region
R6520 T22778 T22777 punct -,terminal
R6521 T22779 T22780 punct (,463
R6522 T22780 T22775 parataxis 463,region
R6523 T22781 T22780 nmod amino,463
R6524 T22782 T22780 nmod acid,463
R6525 T22783 T22780 prep to,463
R6526 T22784 T22783 pobj 542,to
R6527 T22785 T22780 punct ),463
R6528 T22786 T22767 punct .,is
R6529 T22788 T22789 advcl Taken,is
R6530 T22790 T22788 advmod together,Taken
R6531 T22791 T22789 punct ", ",is
R6532 T22792 T22793 compound mr,s
R6533 T22793 T22789 nsubj s,is
R6534 T22794 T22793 punct -,s
R6535 T22795 T22796 det a,molecule
R6536 T22796 T22789 attr molecule,is
R6537 T22797 T22796 amod novel,molecule
R6538 T22798 T22796 compound repressor,molecule
R6539 T22799 T22800 advmod possibly,involved
R6540 T22800 T22796 acl involved,molecule
R6541 T22801 T22800 prep in,involved
R6542 T22802 T22803 det the,development
R6543 T22803 T22801 pobj development,in
R6544 T22804 T22803 prep of,development
R6545 T22805 T22806 amod retinal,photoreceptors
R6546 T22806 T22804 pobj photoreceptors,of
R6547 T22807 T22806 cc and,photoreceptors
R6548 T22808 T22809 amod pineal,gland
R6549 T22809 T22806 conj gland,photoreceptors
R6550 T22810 T22789 punct .,is
R6551 T23218 T23217 prep of,Isolation
R6552 T23219 T23220 compound mouse,cDNA
R6553 T23220 T23218 pobj cDNA,of
R6554 T23221 T23222 compound mr,s
R6555 T23222 T23220 compound s,cDNA
R6556 T23223 T23222 punct -,s
R6557 T23225 T23226 nsubj We,used
R6558 T23227 T23228 det the,method
R6559 T23228 T23226 dobj method,used
R6560 T23229 T23228 compound bioinformatics,method
R6561 T23230 T23231 compound Digital,Display
R6562 T23231 T23228 appos Display,method
R6563 T23232 T23231 compound Differential,Display
R6564 T23233 T23234 punct (,NCBI
R6565 T23234 T23228 parataxis NCBI,method
R6566 T23235 T23234 punct ", ",NCBI
R6567 T23236 T23234 npadvmod UniGene,NCBI
R6568 T23237 T23234 punct ),NCBI
R6569 T23238 T23239 aux to,screen
R6570 T23239 T23226 advcl screen,used
R6571 T23240 T23241 amod novel,genes
R6572 T23241 T23239 dobj genes,screen
R6573 T23242 T23241 compound mouse,genes
R6574 T23243 T23241 acl expressed,genes
R6575 T23244 T23243 advmod preferentially,expressed
R6576 T23245 T23243 prep in,expressed
R6577 T23246 T23247 det the,retina
R6578 T23247 T23245 pobj retina,in
R6579 T23248 T23226 punct .,used
R6580 T23250 T23251 nsubj Some,were
R6581 T23252 T23250 prep of,Some
R6582 T23253 T23254 det the,clusters
R6583 T23254 T23252 pobj clusters,of
R6584 T23255 T23250 prep in,Some
R6585 T23256 T23257 det the,database
R6586 T23257 T23255 pobj database,in
R6587 T23258 T23257 compound UniGene,database
R6588 T23259 T23251 advmod mainly,were
R6589 T23260 T23251 prep from,were
R6590 T23261 T23262 nmod mouse,cDNAs
R6591 T23262 T23260 pobj cDNAs,from
R6592 T23263 T23262 amod retinal,cDNAs
R6593 T23264 T23251 punct .,were
R6594 T23266 T23267 nummod One,clone
R6595 T23267 T23268 nsubj clone,has
R6596 T23269 T23267 prep in,clone
R6597 T23270 T23271 det these,clusters
R6598 T23271 T23269 pobj clusters,in
R6599 T23272 T23273 punct (,Mm.
R6600 T23273 T23267 parataxis Mm.,clone
R6601 T23274 T23273 punct #,Mm.
R6602 T23275 T23273 nummod 246385,Mm.
R6603 T23276 T23273 punct ),Mm.
R6604 T23277 T23278 det a,homology
R6605 T23278 T23268 dobj homology,has
R6606 T23279 T23278 amod weak,homology
R6607 T23280 T23278 prep with,homology
R6608 T23281 T23282 det the,genes
R6609 T23282 T23280 pobj genes,with
R6610 T23283 T23282 amod polyhomeotic,genes
R6611 T23284 T23282 compound family,genes
R6612 T23285 T23268 punct .,has
R6613 T23287 T23288 det A,fragment
R6614 T23288 T23293 nsubjpass fragment,amplified
R6615 T23289 T23290 nummod 735,bp
R6616 T23290 T23288 compound bp,fragment
R6617 T23291 T23290 punct -,bp
R6618 T23292 T23288 compound cDNA,fragment
R6619 T23294 T23288 prep of,fragment
R6620 T23295 T23296 det this,clone
R6621 T23296 T23294 pobj clone,of
R6622 T23297 T23288 punct ", ",fragment
R6623 T23298 T23288 acl encoding,fragment
R6624 T23299 T23300 nmod amino,140
R6625 T23300 T23298 dobj 140,encoding
R6626 T23301 T23300 nmod acids,140
R6627 T23302 T23303 punct –,384
R6628 T23303 T23300 prep 384,140
R6629 T23304 T23293 auxpass was,amplified
R6630 T23305 T23293 prep by,amplified
R6631 T23306 T23307 compound RT,PCR
R6632 T23307 T23305 pobj PCR,by
R6633 T23308 T23307 punct -,PCR
R6634 T23309 T23293 prep from,amplified
R6635 T23310 T23311 nmod mouse,cDNA
R6636 T23311 T23309 pobj cDNA,from
R6637 T23312 T23311 nmod P0,cDNA
R6638 T23313 T23311 amod retinal,cDNA
R6639 T23314 T23293 punct .,amplified
R6640 T23316 T23317 det This,fragment
R6641 T23317 T23318 nsubjpass fragment,used
R6642 T23319 T23318 auxpass was,used
R6643 T23320 T23318 prep as,used
R6644 T23321 T23322 det the,probe
R6645 T23322 T23320 pobj probe,as
R6646 T23323 T23318 prep for,used
R6647 T23324 T23325 compound library,screening
R6648 T23325 T23323 pobj screening,for
R6649 T23326 T23325 punct ", ",screening
R6650 T23327 T23328 advmod in,situ
R6651 T23328 T23329 amod situ,hybridization
R6652 T23329 T23325 conj hybridization,screening
R6653 T23330 T23329 cc and,hybridization
R6654 T23331 T23332 compound Northern,hybridization
R6655 T23332 T23329 conj hybridization,hybridization
R6656 T23333 T23318 punct .,used
R6657 T23335 T23336 det A,library
R6658 T23336 T23343 nsubjpass library,screened
R6659 T23337 T23336 nmod mouse,library
R6660 T23338 T23336 nmod P0,library
R6661 T23339 T23340 punct -,P3
R6662 T23340 T23338 prep P3,P0
R6663 T23341 T23336 amod retinal,library
R6664 T23342 T23336 compound cDNA,library
R6665 T23344 T23343 auxpass was,screened
R6666 T23345 T23343 advcl using,screened
R6667 T23346 T23347 det this,fragment
R6668 T23347 T23345 dobj fragment,using
R6669 T23348 T23347 compound mouse,fragment
R6670 T23349 T23347 compound cDNA,fragment
R6671 T23350 T23343 punct .,screened
R6672 T23352 T23353 amod Positive,clones
R6673 T23353 T23355 nsubjpass clones,isolated
R6674 T23354 T23353 compound bacteriophage,clones
R6675 T23356 T23355 auxpass were,isolated
R6676 T23357 T23355 cc and,isolated
R6677 T23358 T23359 det the,fragment
R6678 T23359 T23366 nsubjpass fragment,inserted
R6679 T23360 T23361 amod full,length
R6680 T23361 T23359 compound length,fragment
R6681 T23362 T23361 punct -,length
R6682 T23363 T23364 compound mr,s
R6683 T23364 T23359 compound s,fragment
R6684 T23365 T23364 punct -,s
R6685 T23366 T23355 conj inserted,isolated
R6686 T23367 T23366 auxpass was,inserted
R6687 T23368 T23366 prep into,inserted
R6688 T23369 T23368 pobj pBluescriptII,into
R6689 T23370 T23371 punct (,Stratagene
R6690 T23371 T23366 parataxis Stratagene,inserted
R6691 T23372 T23371 punct ),Stratagene
R6692 T23373 T23366 punct .,inserted
R6693 T23375 T23376 compound DNA,sequencing
R6694 T23376 T23377 nsubjpass sequencing,performed
R6695 T23378 T23377 auxpass was,performed
R6696 T23379 T23377 prep on,performed
R6697 T23380 T23381 det both,strands
R6698 T23381 T23379 pobj strands,on
R6699 T23382 T23377 prep by,performed
R6700 T23383 T23384 det the,method
R6701 T23384 T23382 pobj method,by
R6702 T23385 T23384 compound cycle,method
R6703 T23386 T23384 compound sequencing,method
R6704 T23387 T23377 punct .,performed
R6705 T23389 T23390 det The,sequence
R6706 T23390 T23392 nsubjpass sequence,deposited
R6707 T23391 T23390 compound nucleotide,sequence
R6708 T23393 T23390 prep for,sequence
R6709 T23394 T23395 compound mr,s
R6710 T23395 T23397 compound s,gene
R6711 T23396 T23395 punct -,s
R6712 T23397 T23393 pobj gene,for
R6713 T23398 T23392 aux has,deposited
R6714 T23399 T23392 auxpass been,deposited
R6715 T23400 T23392 prep in,deposited
R6716 T23401 T23402 det the,database
R6717 T23402 T23400 pobj database,in
R6718 T23403 T23402 compound GenBank,database
R6719 T23404 T23392 prep under,deposited
R6720 T23405 T23406 nmod GenBank,AY458844
R6721 T23406 T23404 pobj AY458844,under
R6722 T23407 T23406 nmod Accession,AY458844
R6723 T23408 T23406 nmod Number,AY458844
R6724 T23409 T23406 punct #,AY458844
R6725 T23410 T23392 punct .,deposited
R6726 T23508 T23509 advmod In,situ
R6727 T23509 T23510 amod situ,hybridization
R6728 T23512 T23513 det The,fragment
R6729 T23513 T23518 nsubjpass fragment,used
R6730 T23514 T23515 nummod 735,bp
R6731 T23515 T23513 compound bp,fragment
R6732 T23516 T23515 punct -,bp
R6733 T23517 T23513 compound cDNA,fragment
R6734 T23519 T23513 prep of,fragment
R6735 T23520 T23521 compound mr,s
R6736 T23521 T23519 pobj s,of
R6737 T23522 T23521 punct -,s
R6738 T23523 T23513 acl amplified,fragment
R6739 T23524 T23523 prep by,amplified
R6740 T23525 T23526 compound RT,PCR
R6741 T23526 T23524 pobj PCR,by
R6742 T23527 T23526 punct -,PCR
R6743 T23528 T23518 auxpass was,used
R6744 T23529 T23518 prep as,used
R6745 T23530 T23531 det a,probe
R6746 T23531 T23529 pobj probe,as
R6747 T23532 T23518 prep for,used
R6748 T23533 T23534 advmod in,situ
R6749 T23534 T23535 amod situ,hybridization
R6750 T23535 T23532 pobj hybridization,for
R6751 T23536 T23518 punct .,used
R6752 T23538 T23539 advmod In,situ
R6753 T23539 T23540 amod situ,hybridization
R6754 T23540 T23541 nsubjpass hybridization,performed
R6755 T23542 T23541 auxpass was,performed
R6756 T23543 T23544 mark as,described
R6757 T23544 T23541 advcl described,performed
R6758 T23545 T23544 advmod previously,described
R6759 T23546 T23547 punct [,49
R6760 T23547 T23541 parataxis 49,performed
R6761 T23548 T23547 punct ],49
R6762 T23549 T23541 punct .,performed
R6763 T23802 T23803 compound Cell,culture
R6764 T23804 T23803 cc and,culture
R6765 T23805 T23803 conj transfection,culture
R6766 T23807 T23808 compound HEK293T,cells
R6767 T23808 T23809 nsubjpass cells,maintained
R6768 T23810 T23809 auxpass were,maintained
R6769 T23811 T23809 prep at,maintained
R6770 T23812 T23813 nummod 37,°C
R6771 T23813 T23811 pobj °C,at
R6772 T23814 T23809 prep in,maintained
R6773 T23815 T23816 poss Dulbecco,medium
R6774 T23816 T23814 pobj medium,in
R6775 T23817 T23815 case 's,Dulbecco
R6776 T23818 T23816 amod modified,medium
R6777 T23819 T23816 poss Eagle,medium
R6778 T23820 T23819 case 's,Eagle
R6779 T23821 T23816 punct (,medium
R6780 T23822 T23816 appos DMEM,medium
R6781 T23823 T23816 punct ),medium
R6782 T23824 T23816 acl supplemented,medium
R6783 T23825 T23824 prep with,supplemented
R6784 T23826 T23827 nummod 10,%
R6785 T23827 T23828 nmod %,serum
R6786 T23828 T23825 pobj serum,with
R6787 T23829 T23830 amod fetal,bovine
R6788 T23830 T23828 amod bovine,serum
R6789 T23831 T23832 punct (,Sigma
R6790 T23832 T23828 parataxis Sigma,serum
R6791 T23833 T23832 punct ),Sigma
R6792 T23834 T23828 punct ", ",serum
R6793 T23835 T23836 nummod 100,IU
R6794 T23836 T23837 nmod IU,penicillin
R6795 T23837 T23828 conj penicillin,serum
R6796 T23838 T23839 punct /,ml
R6797 T23839 T23836 prep ml,IU
R6798 T23840 T23837 cc and,penicillin
R6799 T23841 T23842 nummod 100,μg
R6800 T23842 T23843 nmod μg,streptomycin
R6801 T23843 T23837 conj streptomycin,penicillin
R6802 T23844 T23845 punct /,ml
R6803 T23845 T23842 prep ml,μg
R6804 T23846 T23809 punct .,maintained
R6805 T23848 T23849 amod Transient,transfection
R6806 T23849 T23850 nsubjpass transfection,carried
R6807 T23851 T23849 prep of,transfection
R6808 T23852 T23853 compound HEK293T,cells
R6809 T23853 T23851 pobj cells,of
R6810 T23854 T23850 auxpass was,carried
R6811 T23855 T23850 prt out,carried
R6812 T23856 T23850 advcl using,carried
R6813 T23857 T23858 compound calcium,phosphate
R6814 T23858 T23859 compound phosphate,method
R6815 T23859 T23856 dobj method,using
R6816 T23860 T23859 cc or,method
R6817 T23861 T23862 compound Fugene6,reagent
R6818 T23862 T23859 conj reagent,method
R6819 T23863 T23862 compound transfection,reagent
R6820 T23864 T23865 punct (,Roche
R6821 T23865 T23862 parataxis Roche,reagent
R6822 T23866 T23865 punct ),Roche
R6823 T23867 T23850 punct .,carried
R6824 T23869 T23870 compound Y79,cells
R6825 T23870 T23872 nsubjpass cells,maintained
R6826 T23871 T23870 compound retinoblastoma,cells
R6827 T23873 T23872 auxpass were,maintained
R6828 T23874 T23872 prep in,maintained
R6829 T23875 T23876 poss Iscove,medium
R6830 T23876 T23874 pobj medium,in
R6831 T23877 T23875 case 's,Iscove
R6832 T23878 T23876 amod modified,medium
R6833 T23879 T23876 poss Dulbecco,medium
R6834 T23880 T23879 case 's,Dulbecco
R6835 T23881 T23876 prep with,medium
R6836 T23882 T23883 nummod 4,mM
R6837 T23883 T23884 compound mM,glutamine
R6838 T23884 T23881 pobj glutamine,with
R6839 T23885 T23884 compound L,glutamine
R6840 T23886 T23884 punct -,glutamine
R6841 T23887 T23884 acl adjusted,glutamine
R6842 T23888 T23889 aux to,contain
R6843 T23889 T23887 advcl contain,adjusted
R6844 T23890 T23891 nummod 1.5,g
R6845 T23891 T23892 nmod g,bicarbonate
R6846 T23892 T23889 dobj bicarbonate,contain
R6847 T23893 T23894 punct /,L
R6848 T23894 T23891 prep L,g
R6849 T23895 T23892 compound sodium,bicarbonate
R6850 T23896 T23892 punct ", ",bicarbonate
R6851 T23897 T23898 nummod 20,%
R6852 T23898 T23899 compound %,FBS
R6853 T23899 T23892 npadvmod FBS,bicarbonate
R6854 T23900 T23872 punct .,maintained
R6855 T23902 T23903 amod Transient,transfection
R6856 T23903 T23904 nsubjpass transfection,carried
R6857 T23905 T23904 auxpass was,carried
R6858 T23906 T23904 prt out,carried
R6859 T23907 T23904 advcl using,carried
R6860 T23908 T23909 compound TransIt,LT1
R6861 T23909 T23907 dobj LT1,using
R6862 T23910 T23911 punct (,Mirus
R6863 T23911 T23909 parataxis Mirus,LT1
R6864 T23912 T23911 punct ),Mirus
R6865 T23913 T23904 punct .,carried
R6866 T24169 T24170 compound Northern,blot
R6867 T24170 T24172 compound blot,analysis
R6868 T24171 T24170 punct -,blot
R6869 T24174 T24175 nsubjpass RNA,extracted
R6870 T24176 T24175 auxpass was,extracted
R6871 T24177 T24175 prep from,extracted
R6872 T24178 T24179 det the,tissues
R6873 T24179 T24177 pobj tissues,from
R6874 T24180 T24179 prep of,tissues
R6875 T24181 T24182 amod adult,mice
R6876 T24182 T24180 pobj mice,of
R6877 T24183 T24175 advcl using,extracted
R6878 T24184 T24183 dobj Trizol,using
R6879 T24185 T24186 punct (,Invitrogen
R6880 T24186 T24184 parataxis Invitrogen,Trizol
R6881 T24187 T24186 punct ),Invitrogen
R6882 T24188 T24175 punct .,extracted
R6883 T24190 T24191 det A,μg
R6884 T24191 T24193 nsubjpass μg,electrophoresed
R6885 T24192 T24191 nummod 5,μg
R6886 T24194 T24191 prep of,μg
R6887 T24195 T24196 amod total,RNA
R6888 T24196 T24194 pobj RNA,of
R6889 T24197 T24193 auxpass was,electrophoresed
R6890 T24198 T24193 prep in,electrophoresed
R6891 T24199 T24200 det a,gel
R6892 T24200 T24198 pobj gel,in
R6893 T24201 T24202 nummod 1.0,%
R6894 T24202 T24200 compound %,gel
R6895 T24203 T24204 compound agarose,formaldehyde
R6896 T24204 T24200 compound formaldehyde,gel
R6897 T24205 T24204 punct -,formaldehyde
R6898 T24206 T24193 cc and,electrophoresed
R6899 T24207 T24193 conj transferred,electrophoresed
R6900 T24208 T24207 prep to,transferred
R6901 T24209 T24210 det a,membrane
R6902 T24210 T24208 pobj membrane,to
R6903 T24211 T24210 compound nylon,membrane
R6904 T24212 T24213 punct (,GT
R6905 T24213 T24210 parataxis GT,membrane
R6906 T24214 T24215 compound Zeta,Probe
R6907 T24215 T24213 compound Probe,GT
R6908 T24216 T24215 punct -,Probe
R6909 T24217 T24213 punct ", ",GT
R6910 T24218 T24219 compound Bio,Rad
R6911 T24219 T24213 npadvmod Rad,GT
R6912 T24220 T24219 punct -,Rad
R6913 T24221 T24213 punct ),GT
R6914 T24222 T24193 punct .,electrophoresed
R6915 T24224 T24225 det The,fragment
R6916 T24225 T24230 nsubjpass fragment,used
R6917 T24226 T24227 nummod 735,bp
R6918 T24227 T24225 compound bp,fragment
R6919 T24228 T24227 punct -,bp
R6920 T24229 T24225 compound cDNA,fragment
R6921 T24231 T24225 acl encoding,fragment
R6922 T24232 T24233 det the,domain
R6923 T24233 T24231 dobj domain,encoding
R6924 T24234 T24233 compound SAM,domain
R6925 T24235 T24233 prep of,domain
R6926 T24236 T24237 compound mr,s
R6927 T24237 T24235 pobj s,of
R6928 T24238 T24237 punct -,s
R6929 T24239 T24230 auxpass was,used
R6930 T24240 T24230 prep as,used
R6931 T24241 T24242 det a,probe
R6932 T24242 T24240 pobj probe,as
R6933 T24243 T24230 prep for,used
R6934 T24244 T24243 pobj hybridization,for
R6935 T24245 T24230 punct .,used
R6936 T24247 T24248 nsubjpass Hybridization,performed
R6937 T24249 T24248 auxpass was,performed
R6938 T24250 T24248 prep according,performed
R6939 T24251 T24250 prep to,according
R6940 T24252 T24253 det the,manufacturer
R6941 T24253 T24254 poss manufacturer,protocol
R6942 T24254 T24251 pobj protocol,to
R6943 T24255 T24253 case 's,manufacturer
R6944 T24256 T24248 punct .,performed
R6945 T24258 T24259 nsubjpass Washes,performed
R6946 T24260 T24258 prep with,Washes
R6947 T24261 T24262 amod increasing,stringency
R6948 T24262 T24260 pobj stringency,with
R6949 T24263 T24259 auxpass were,performed
R6950 T24264 T24259 punct ", ",performed
R6951 T24265 T24266 det the,last
R6952 T24266 T24267 nsubj last,being
R6953 T24267 T24259 advcl being,performed
R6954 T24268 T24267 prep at,being
R6955 T24269 T24270 nummod 50,°C
R6956 T24270 T24268 pobj °C,at
R6957 T24271 T24267 prep in,being
R6958 T24272 T24273 nummod 0.1,citrate
R6959 T24273 T24271 pobj citrate,in
R6960 T24274 T24272 punct ×,0.1
R6961 T24275 T24273 amod standard,citrate
R6962 T24276 T24273 compound saline,citrate
R6963 T24277 T24278 punct /,%
R6964 T24278 T24273 prep %,citrate
R6965 T24279 T24278 nummod 0.1,%
R6966 T24280 T24281 compound sodium,sulfate
R6967 T24281 T24278 appos sulfate,%
R6968 T24282 T24281 compound dodecyl,sulfate
R6969 T24283 T24281 punct (,sulfate
R6970 T24284 T24281 appos SDS,sulfate
R6971 T24285 T24259 punct ),performed
R6972 T24286 T24259 punct .,performed
R6973 T24536 T24537 compound RT,PCR
R6974 T24537 T24539 compound PCR,analysis
R6975 T24538 T24537 punct -,PCR
R6976 T24541 T24542 amod Total,RNA
R6977 T24542 T24543 nsubjpass RNA,isolated
R6978 T24544 T24543 auxpass was,isolated
R6979 T24545 T24543 prep from,isolated
R6980 T24546 T24547 det each,tissue
R6981 T24547 T24545 pobj tissue,from
R6982 T24548 T24543 advcl using,isolated
R6983 T24549 T24548 dobj Trizol,using
R6984 T24550 T24543 punct .,isolated
R6985 T24552 T24553 det A,μg
R6986 T24553 T24555 nsubjpass μg,transcribed
R6987 T24554 T24553 nummod 1,μg
R6988 T24556 T24553 prep of,μg
R6989 T24557 T24558 amod total,RNA
R6990 T24558 T24556 pobj RNA,of
R6991 T24559 T24555 auxpass was,transcribed
R6992 T24560 T24555 amod reverse,transcribed
R6993 T24561 T24555 advcl using,transcribed
R6994 T24562 T24561 dobj SuperscriptII,using
R6995 T24563 T24564 punct (,Invitrogen
R6996 T24564 T24562 parataxis Invitrogen,SuperscriptII
R6997 T24565 T24564 punct ),Invitrogen
R6998 T24566 T24555 punct .,transcribed
R6999 T24568 T24569 compound RT,PCR
R7000 T24569 T24571 compound PCR,primers
R7001 T24570 T24569 punct -,PCR
R7002 T24571 T24572 nsubj primers,were
R7003 T24573 T24571 punct ", ",primers
R7004 T24574 T24575 dep which,span
R7005 T24575 T24571 relcl span,primers
R7006 T24576 T24575 dobj introns,span
R7007 T24577 T24571 punct ", ",primers
R7008 T24578 T24571 prep for,primers
R7009 T24579 T24578 pobj detection,for
R7010 T24580 T24579 prep of,detection
R7011 T24581 T24582 compound mr,s
R7012 T24582 T24584 compound s,cDNA
R7013 T24583 T24582 punct -,s
R7014 T24584 T24580 pobj cDNA,of
R7015 T24585 T24586 nummod 5,TGTCCAGCCCAGCCAACCCAAGGAGACGACA
R7016 T24586 T24572 attr TGTCCAGCCCAGCCAACCCAAGGAGACGACA,were
R7017 T24587 T24585 punct ',5
R7018 T24588 T24586 punct -,TGTCCAGCCCAGCCAACCCAAGGAGACGACA
R7019 T24589 T24586 punct -,TGTCCAGCCCAGCCAACCCAAGGAGACGACA
R7020 T24590 T24586 nummod 3,TGTCCAGCCCAGCCAACCCAAGGAGACGACA
R7021 T24591 T24586 punct ',TGTCCAGCCCAGCCAACCCAAGGAGACGACA
R7022 T24592 T24586 cc and,TGTCCAGCCCAGCCAACCCAAGGAGACGACA
R7023 T24593 T24594 nummod 5,TGTGGTCTCCTCATCAGTGAAGA
R7024 T24594 T24586 conj TGTGGTCTCCTCATCAGTGAAGA,TGTCCAGCCCAGCCAACCCAAGGAGACGACA
R7025 T24595 T24593 punct ',5
R7026 T24596 T24594 punct -,TGTGGTCTCCTCATCAGTGAAGA
R7027 T24597 T24594 punct -,TGTGGTCTCCTCATCAGTGAAGA
R7028 T24598 T24594 nummod 3,TGTGGTCTCCTCATCAGTGAAGA
R7029 T24599 T24594 punct ',TGTGGTCTCCTCATCAGTGAAGA
R7030 T24600 T24572 punct .,were
R7031 T24602 T24603 compound Product,size
R7032 T24603 T24604 nsubj size,was
R7033 T24605 T24606 nummod 292,bp
R7034 T24606 T24604 attr bp,was
R7035 T24607 T24608 punct (,965
R7036 T24608 T24604 parataxis 965,was
R7037 T24609 T24608 nmod positions,965
R7038 T24610 T24611 punct –,1256
R7039 T24611 T24608 prep 1256,965
R7040 T24612 T24608 prep of,965
R7041 T24613 T24614 nmod Genbank,AY458844
R7042 T24614 T24612 pobj AY458844,of
R7043 T24615 T24614 nmod Accession,AY458844
R7044 T24616 T24614 nmod Number,AY458844
R7045 T24617 T24614 punct #,AY458844
R7046 T24618 T24608 punct ),965
R7047 T24619 T24604 punct .,was
R7048 T24621 T24622 compound Primer,pairs
R7049 T24622 T24623 nsubj pairs,were
R7050 T24624 T24622 prep for,pairs
R7051 T24625 T24626 compound mouse,G3PDH
R7052 T24626 T24624 pobj G3PDH,for
R7053 T24627 T24628 nummod 5,ACCACAGTCCATGCCATCAC
R7054 T24628 T24623 attr ACCACAGTCCATGCCATCAC,were
R7055 T24629 T24627 punct ',5
R7056 T24630 T24628 punct -,ACCACAGTCCATGCCATCAC
R7057 T24631 T24628 punct -,ACCACAGTCCATGCCATCAC
R7058 T24632 T24628 nummod 3,ACCACAGTCCATGCCATCAC
R7059 T24633 T24628 punct ',ACCACAGTCCATGCCATCAC
R7060 T24634 T24628 cc and,ACCACAGTCCATGCCATCAC
R7061 T24635 T24636 nummod 5,TCCACCACCCTGTTGCTGTA
R7062 T24636 T24628 conj TCCACCACCCTGTTGCTGTA,ACCACAGTCCATGCCATCAC
R7063 T24637 T24635 punct ',5
R7064 T24638 T24636 punct -,TCCACCACCCTGTTGCTGTA
R7065 T24639 T24636 punct -,TCCACCACCCTGTTGCTGTA
R7066 T24640 T24636 nummod 3,TCCACCACCCTGTTGCTGTA
R7067 T24641 T24636 punct ',TCCACCACCCTGTTGCTGTA
R7068 T24642 T24643 dep which,amplified
R7069 T24643 T24628 relcl amplified,ACCACAGTCCATGCCATCAC
R7070 T24644 T24645 det a,product
R7071 T24645 T24643 dobj product,amplified
R7072 T24646 T24647 nummod 452,bp
R7073 T24647 T24645 compound bp,product
R7074 T24648 T24647 punct -,bp
R7075 T24649 T24650 punct (,587
R7076 T24650 T24643 parataxis 587,amplified
R7077 T24651 T24650 nmod positions,587
R7078 T24652 T24653 punct –,1038
R7079 T24653 T24650 prep 1038,587
R7080 T24654 T24650 prep of,587
R7081 T24655 T24656 nmod Genbank,BC85275
R7082 T24656 T24654 pobj BC85275,of
R7083 T24657 T24656 nmod Accession,BC85275
R7084 T24658 T24656 nmod Number,BC85275
R7085 T24659 T24656 punct #,BC85275
R7086 T24660 T24650 punct ),587
R7087 T24661 T24623 punct .,were
R7088 T25116 T25117 nmod Yeast,screening
R7089 T25118 T25119 nummod two,hybrid
R7090 T25119 T25117 compound hybrid,screening
R7091 T25120 T25119 punct -,hybrid
R7092 T25121 T25117 cc and,screening
R7093 T25122 T25123 compound GAL4,assay
R7094 T25123 T25117 conj assay,screening
R7095 T25125 T25126 nsubj We,carried
R7096 T25127 T25126 prt out,carried
R7097 T25128 T25129 nmod yeast,experiments
R7098 T25129 T25126 dobj experiments,carried
R7099 T25130 T25131 nummod two,hybrid
R7100 T25131 T25129 compound hybrid,experiments
R7101 T25132 T25131 punct -,hybrid
R7102 T25133 T25126 advcl using,carried
R7103 T25134 T25135 det the,system
R7104 T25135 T25133 dobj system,using
R7105 T25136 T25135 nmod MATCHMAKER,system
R7106 T25137 T25135 nmod GAL4,system
R7107 T25138 T25139 nummod two,hybrid
R7108 T25139 T25135 compound hybrid,system
R7109 T25140 T25139 punct -,hybrid
R7110 T25141 T25135 nummod 3,system
R7111 T25142 T25143 punct (,Bioscience
R7112 T25143 T25135 parataxis Bioscience,system
R7113 T25144 T25143 compound BD,Bioscience
R7114 T25145 T25143 punct ),Bioscience
R7115 T25146 T25147 mark as,recommended
R7116 T25147 T25126 advcl recommended,carried
R7117 T25148 T25147 agent by,recommended
R7118 T25149 T25150 det the,manufacturer
R7119 T25150 T25148 pobj manufacturer,by
R7120 T25151 T25126 punct .,carried
R7121 T25153 T25154 nsubj We,cloned
R7122 T25155 T25156 det the,s
R7123 T25156 T25154 dobj s,cloned
R7124 T25157 T25158 amod full,length
R7125 T25158 T25156 compound length,s
R7126 T25159 T25158 punct -,length
R7127 T25160 T25156 compound mr,s
R7128 T25161 T25156 punct -,s
R7129 T25162 T25154 prep into,cloned
R7130 T25163 T25164 det the,vector
R7131 T25164 T25162 pobj vector,into
R7132 T25165 T25164 compound pGBKT7,vector
R7133 T25166 T25154 cc and,cloned
R7134 T25167 T25154 conj used,cloned
R7135 T25168 T25167 dobj it,used
R7136 T25169 T25170 aux to,screen
R7137 T25170 T25167 advcl screen,used
R7138 T25171 T25172 det a,library
R7139 T25172 T25170 dobj library,screen
R7140 T25173 T25172 prep of,library
R7141 T25174 T25175 nmod mouse,cDNAs
R7142 T25175 T25173 pobj cDNAs,of
R7143 T25176 T25175 nmod P0,cDNAs
R7144 T25177 T25178 punct -,P3
R7145 T25178 T25176 prep P3,P0
R7146 T25179 T25175 amod retinal,cDNAs
R7147 T25180 T25175 prep in,cDNAs
R7148 T25181 T25182 det the,vector
R7149 T25182 T25180 pobj vector,in
R7150 T25183 T25182 compound pGADT7,vector
R7151 T25184 T25154 punct .,cloned
R7152 T25186 T25187 nsubj Transformants,picked
R7153 T25188 T25189 dep that,conferred
R7154 T25189 T25186 relcl conferred,Transformants
R7155 T25190 T25189 dobj growth,conferred
R7156 T25191 T25187 aux were,picked
R7157 T25192 T25187 punct ", ",picked
R7158 T25193 T25187 conj isolated,picked
R7159 T25194 T25193 cc and,isolated
R7160 T25195 T25193 conj re-introduced,isolated
R7161 T25196 T25195 prep with,re-introduced
R7162 T25197 T25198 det the,bait
R7163 T25198 T25196 pobj bait,with
R7164 T25199 T25195 prep into,re-introduced
R7165 T25200 T25199 pobj AH109,into
R7166 T25201 T25202 aux to,confirm
R7167 T25202 T25187 advcl confirm,picked
R7168 T25203 T25202 dobj interaction,confirm
R7169 T25204 T25187 punct .,picked
R7170 T25206 T25207 compound Plasmid,DNA
R7171 T25207 T25208 nsubjpass DNA,isolated
R7172 T25209 T25208 auxpass was,isolated
R7173 T25210 T25208 prep from,isolated
R7174 T25211 T25210 pobj yeast,from
R7175 T25212 T25208 advcl using,isolated
R7176 T25213 T25214 compound RPM,Kit
R7177 T25214 T25212 dobj Kit,using
R7178 T25215 T25216 compound Yeast,Plasmid
R7179 T25216 T25217 compound Plasmid,Isolation
R7180 T25217 T25214 compound Isolation,Kit
R7181 T25218 T25219 punct (,Qbiogene
R7182 T25219 T25214 parataxis Qbiogene,Kit
R7183 T25220 T25219 punct ),Qbiogene
R7184 T25221 T25208 punct .,isolated
R7185 T25223 T25224 prep In,picked
R7186 T25225 T25223 pobj order,In
R7187 T25226 T25227 aux to,confirm
R7188 T25227 T25225 acl confirm,order
R7189 T25228 T25229 det the,interactions
R7190 T25229 T25227 dobj interactions,confirm
R7191 T25230 T25229 compound protein,interactions
R7192 T25231 T25224 punct ", ",picked
R7193 T25232 T25233 amod single,colonies
R7194 T25233 T25224 nsubjpass colonies,picked
R7195 T25234 T25224 auxpass were,picked
R7196 T25235 T25224 cc and,picked
R7197 T25236 T25224 conj grown,picked
R7198 T25237 T25236 advmod individually,grown
R7199 T25238 T25236 prep in,grown
R7200 T25239 T25240 amod synthetic,media
R7201 T25240 T25238 pobj media,in
R7202 T25241 T25240 amod complete,media
R7203 T25242 T25240 acl lacking,media
R7204 T25243 T25244 nmod leucine,tryptophan
R7205 T25244 T25242 dobj tryptophan,lacking
R7206 T25245 T25244 punct ", ",tryptophan
R7207 T25246 T25242 cc and,lacking
R7208 T25247 T25242 conj containing,lacking
R7209 T25248 T25249 compound X,gal
R7210 T25249 T25247 dobj gal,containing
R7211 T25250 T25249 punct -,gal
R7212 T25251 T25224 punct .,picked
R7213 T25253 T25254 prep After,selected
R7214 T25255 T25256 quantmod 4,5
R7215 T25256 T25258 nummod 5,days
R7216 T25257 T25256 punct –,5
R7217 T25258 T25253 pobj days,After
R7218 T25259 T25254 punct ", ",selected
R7219 T25260 T25261 compound X,gal
R7220 T25261 T25263 npadvmod gal,positive
R7221 T25262 T25261 compound -,gal
R7222 T25263 T25264 amod positive,clones
R7223 T25264 T25254 nsubjpass clones,selected
R7224 T25265 T25254 auxpass were,selected
R7225 T25266 T25254 cc and,selected
R7226 T25267 T25254 conj analyzed,selected
R7227 T25268 T25254 punct .,selected
R7228 T25270 T25271 aux To,test
R7229 T25271 T25272 advcl test,subcloned
R7230 T25273 T25274 compound self,interaction
R7231 T25274 T25271 dobj interaction,test
R7232 T25275 T25274 punct -,interaction
R7233 T25276 T25274 prep of,interaction
R7234 T25277 T25278 compound mr,s
R7235 T25278 T25276 pobj s,of
R7236 T25279 T25278 punct -,s
R7237 T25280 T25272 punct ", ",subcloned
R7238 T25281 T25272 nsubj we,subcloned
R7239 T25282 T25283 det the,length
R7240 T25283 T25272 dobj length,subcloned
R7241 T25284 T25283 amod full,length
R7242 T25285 T25283 punct -,length
R7243 T25286 T25283 punct ", ",length
R7244 T25287 T25288 compound N,terminus
R7245 T25288 T25283 conj terminus,length
R7246 T25289 T25288 punct -,terminus
R7247 T25290 T25291 punct (,1
R7248 T25291 T25288 parataxis 1,terminus
R7249 T25292 T25291 nmod amino,1
R7250 T25293 T25291 nmod acids,1
R7251 T25294 T25295 punct –,400
R7252 T25295 T25291 prep 400,1
R7253 T25296 T25291 punct ),1
R7254 T25297 T25288 cc and,terminus
R7255 T25298 T25299 compound C,terminus
R7256 T25299 T25288 conj terminus,terminus
R7257 T25300 T25299 punct -,terminus
R7258 T25301 T25302 punct (,391
R7259 T25302 T25299 parataxis 391,terminus
R7260 T25303 T25302 nmod amino,391
R7261 T25304 T25302 nmod acids,391
R7262 T25305 T25306 punct –,542
R7263 T25306 T25302 prep 542,391
R7264 T25307 T25302 punct ),391
R7265 T25308 T25283 prep of,length
R7266 T25309 T25310 compound mr,s
R7267 T25310 T25308 pobj s,of
R7268 T25311 T25310 punct -,s
R7269 T25312 T25272 prep into,subcloned
R7270 T25313 T25314 nmod pGBKT7,vectors
R7271 T25314 T25312 pobj vectors,into
R7272 T25315 T25313 cc and,pGBKT7
R7273 T25316 T25313 conj pGADT7,pGBKT7
R7274 T25317 T25272 punct .,subcloned
R7275 T25319 T25320 nsubj We,assessed
R7276 T25321 T25320 dobj interactions,assessed
R7277 T25322 T25320 prep by,assessed
R7278 T25323 T25322 pcomp scoring,by
R7279 T25324 T25325 amod blue,color
R7280 T25325 T25323 dobj color,scoring
R7281 T25326 T25323 prep on,scoring
R7282 T25327 T25326 pobj plates,on
R7283 T25328 T25327 prep of,plates
R7284 T25329 T25328 pobj medium,of
R7285 T25330 T25327 acl containing,plates
R7286 T25331 T25332 compound X,gal
R7287 T25332 T25330 dobj gal,containing
R7288 T25333 T25332 punct -,gal
R7289 T25334 T25320 punct .,assessed
R7290 T26198 T26199 compound Immunoprecipitation,assay
R7291 T26201 T26202 prep For,subcloned
R7292 T26203 T26204 compound immunoprecipitation,assay
R7293 T26204 T26201 pobj assay,For
R7294 T26205 T26202 punct ", ",subcloned
R7295 T26206 T26207 nummod 4,hemagglutinin
R7296 T26207 T26209 npadvmod hemagglutinin,tagged
R7297 T26208 T26206 punct ×,4
R7298 T26209 T26217 amod tagged,fragment
R7299 T26210 T26207 punct (,hemagglutinin
R7300 T26211 T26207 appos HA,hemagglutinin
R7301 T26212 T26207 punct ),hemagglutinin
R7302 T26213 T26207 cc or,hemagglutinin
R7303 T26214 T26215 nummod 3,Flag
R7304 T26215 T26207 conj Flag,hemagglutinin
R7305 T26216 T26214 punct ×,3
R7306 T26217 T26202 nsubjpass fragment,subcloned
R7307 T26218 T26217 compound cDNA,fragment
R7308 T26219 T26217 acl encoding,fragment
R7309 T26220 T26221 amod full,length
R7310 T26221 T26223 compound length,s
R7311 T26222 T26221 punct -,length
R7312 T26223 T26219 dobj s,encoding
R7313 T26224 T26223 compound mr,s
R7314 T26225 T26223 punct -,s
R7315 T26226 T26227 punct (,HA
R7316 T26227 T26223 parataxis HA,s
R7317 T26228 T26227 amod full,HA
R7318 T26229 T26227 punct -,HA
R7319 T26230 T26227 cc and,HA
R7320 T26231 T26232 compound Flag,mrs
R7321 T26232 T26227 conj mrs,HA
R7322 T26233 T26232 punct -,mrs
R7323 T26234 T26227 punct ),HA
R7324 T26235 T26223 punct ", ",s
R7325 T26236 T26237 det a,fragment
R7326 T26237 T26223 conj fragment,s
R7327 T26238 T26237 compound cDNA,fragment
R7328 T26239 T26237 acl encoding,fragment
R7329 T26240 T26241 nmod amino,1
R7330 T26241 T26239 dobj 1,encoding
R7331 T26242 T26241 nmod acids,1
R7332 T26243 T26244 punct –,400
R7333 T26244 T26241 prep 400,1
R7334 T26245 T26246 punct (,HA
R7335 T26246 T26237 parataxis HA,fragment
R7336 T26247 T26246 compound ΔSAM,HA
R7337 T26248 T26246 punct -,HA
R7338 T26249 T26246 cc and,HA
R7339 T26250 T26251 compound Flag,ΔSAM
R7340 T26251 T26246 conj ΔSAM,HA
R7341 T26252 T26251 punct -,ΔSAM
R7342 T26253 T26246 punct ),HA
R7343 T26254 T26237 punct ", ",fragment
R7344 T26255 T26237 cc and,fragment
R7345 T26256 T26257 det a,fragment
R7346 T26257 T26237 conj fragment,fragment
R7347 T26258 T26257 compound cDNA,fragment
R7348 T26259 T26257 acl encoding,fragment
R7349 T26260 T26261 nmod amino,400
R7350 T26261 T26259 dobj 400,encoding
R7351 T26262 T26261 nmod acids,400
R7352 T26263 T26264 punct –,542
R7353 T26264 T26261 prep 542,400
R7354 T26265 T26266 punct (,SAM
R7355 T26266 T26257 parataxis SAM,fragment
R7356 T26267 T26266 compound Flag,SAM
R7357 T26268 T26266 punct -,SAM
R7358 T26269 T26266 punct ),SAM
R7359 T26270 T26202 auxpass were,subcloned
R7360 T26271 T26202 prep into,subcloned
R7361 T26272 T26273 det the,vector
R7362 T26273 T26271 pobj vector,into
R7363 T26274 T26273 nmod pcDNA3,vector
R7364 T26275 T26276 punct (,Invitrogen
R7365 T26276 T26274 parataxis Invitrogen,pcDNA3
R7366 T26277 T26276 punct ),Invitrogen
R7367 T26278 T26273 compound expression,vector
R7368 T26279 T26202 punct .,subcloned
R7369 T26281 T26282 nsubj We,constructed
R7370 T26283 T26282 advmod also,constructed
R7371 T26284 T26285 nummod two,mutants
R7372 T26285 T26282 dobj mutants,constructed
R7373 T26286 T26287 npadvmod site,directed
R7374 T26287 T26285 amod directed,mutants
R7375 T26288 T26287 punct -,directed
R7376 T26289 T26285 punct ", ",mutants
R7377 T26290 T26291 compound Flag,W404A
R7378 T26291 T26285 appos W404A,mutants
R7379 T26292 T26291 punct -,W404A
R7380 T26293 T26291 cc and,W404A
R7381 T26294 T26295 compound Flag,G453A
R7382 T26295 T26291 conj G453A,W404A
R7383 T26296 T26295 punct -,G453A
R7384 T26297 T26282 punct ", ",constructed
R7385 T26298 T26282 advcl using,constructed
R7386 T26299 T26298 dobj PCR,using
R7387 T26300 T26282 punct .,constructed
R7388 T26302 T26303 nsubjpass Each,introduced
R7389 T26304 T26302 prep of,Each
R7390 T26305 T26306 det these,mutations
R7391 T26306 T26304 pobj mutations,of
R7392 T26307 T26303 auxpass was,introduced
R7393 T26308 T26303 advmod also,introduced
R7394 T26309 T26303 prep into,introduced
R7395 T26310 T26309 pobj Flag,into
R7396 T26311 T26310 punct -,Flag
R7397 T26312 T26313 compound mr,s
R7398 T26313 T26310 appos s,Flag
R7399 T26314 T26313 punct -,s
R7400 T26315 T26303 punct .,introduced
R7401 T26317 T26318 nsubj We,transfected
R7402 T26319 T26320 compound HEK293T,cells
R7403 T26320 T26318 dobj cells,transfected
R7404 T26321 T26318 prep with,transfected
R7405 T26322 T26323 nummod 5,μg
R7406 T26323 T26321 pobj μg,with
R7407 T26324 T26323 prep of,μg
R7408 T26325 T26326 compound plasmid,DNA
R7409 T26326 T26324 pobj DNA,of
R7410 T26327 T26323 prep per,μg
R7411 T26328 T26329 nummod 6,cm
R7412 T26329 T26330 compound cm,dish
R7413 T26330 T26327 pobj dish,per
R7414 T26331 T26318 prep by,transfected
R7415 T26332 T26333 compound calcium,phosphate
R7416 T26333 T26334 compound phosphate,method
R7417 T26334 T26331 pobj method,by
R7418 T26335 T26318 punct .,transfected
R7419 T26337 T26338 advmod Approximately,48
R7420 T26338 T26339 nummod 48,hr
R7421 T26339 T26340 npadvmod hr,after
R7422 T26340 T26341 prep after,harvested
R7423 T26342 T26340 pobj transfection,after
R7424 T26343 T26341 punct ", ",harvested
R7425 T26344 T26341 nsubjpass cells,harvested
R7426 T26345 T26341 auxpass were,harvested
R7427 T26346 T26341 prep in,harvested
R7428 T26347 T26348 compound immunoprecipitation,buffer
R7429 T26348 T26346 pobj buffer,in
R7430 T26349 T26350 punct (,HCl
R7431 T26350 T26341 parataxis HCl,harvested
R7432 T26351 T26352 nummod 50,mM
R7433 T26352 T26350 compound mM,HCl
R7434 T26353 T26350 compound Tris,HCl
R7435 T26354 T26350 punct -,HCl
R7436 T26355 T26356 punct [,pH
R7437 T26356 T26350 parataxis pH,HCl
R7438 T26357 T26356 nummod 7.5,pH
R7439 T26358 T26356 punct ],pH
R7440 T26359 T26350 punct ", ",HCl
R7441 T26360 T26361 nummod 1,mM
R7442 T26361 T26362 compound mM,EDTA
R7443 T26362 T26350 appos EDTA,HCl
R7444 T26363 T26350 punct ", ",HCl
R7445 T26364 T26365 nummod 2,mM
R7446 T26365 T26366 compound mM,MgCl2
R7447 T26366 T26350 appos MgCl2,HCl
R7448 T26367 T26350 punct ", ",HCl
R7449 T26368 T26369 nummod 150,mM
R7450 T26369 T26370 compound mM,NaCl
R7451 T26370 T26350 appos NaCl,HCl
R7452 T26371 T26350 punct ", ",HCl
R7453 T26372 T26373 nummod 1,mM
R7454 T26373 T26374 compound mM,fluoride
R7455 T26374 T26350 appos fluoride,HCl
R7456 T26375 T26374 compound phenylmethylsulfonyl,fluoride
R7457 T26376 T26377 punct [,Wako
R7458 T26377 T26374 parataxis Wako,fluoride
R7459 T26378 T26377 punct ],Wako
R7460 T26379 T26350 punct ", ",HCl
R7461 T26380 T26381 nummod 1,%
R7462 T26381 T26382 compound %,P
R7463 T26382 T26350 appos P,HCl
R7464 T26383 T26382 compound Nonidet,P
R7465 T26384 T26382 punct -,P
R7466 T26385 T26382 nummod 40,P
R7467 T26386 T26350 punct ", ",HCl
R7468 T26387 T26388 nummod 10,%
R7469 T26388 T26389 compound %,glycerol
R7470 T26389 T26350 appos glycerol,HCl
R7471 T26390 T26350 punct ),HCl
R7472 T26391 T26341 prep in,harvested
R7473 T26392 T26393 det the,presence
R7474 T26393 T26391 pobj presence,in
R7475 T26394 T26393 prep of,presence
R7476 T26395 T26396 compound protease,inhibitor
R7477 T26396 T26397 compound inhibitor,tablets
R7478 T26397 T26394 pobj tablets,of
R7479 T26398 T26397 compound cocktail,tablets
R7480 T26399 T26400 punct (,Roche
R7481 T26400 T26397 parataxis Roche,tablets
R7482 T26401 T26400 punct ),Roche
R7483 T26402 T26341 punct .,harvested
R7484 T26404 T26405 prep For,mixed
R7485 T26406 T26407 det each,reaction
R7486 T26407 T26404 pobj reaction,For
R7487 T26408 T26405 punct ", ",mixed
R7488 T26409 T26410 nummod 1,mg
R7489 T26410 T26405 nsubjpass mg,mixed
R7490 T26411 T26410 prep of,mg
R7491 T26412 T26413 compound cell,lysate
R7492 T26413 T26411 pobj lysate,of
R7493 T26414 T26405 auxpass was,mixed
R7494 T26415 T26405 prep with,mixed
R7495 T26416 T26417 nummod 0.5,μg
R7496 T26417 T26415 pobj μg,with
R7497 T26418 T26417 prep of,μg
R7498 T26419 T26420 amod anti-Flag,antibody
R7499 T26420 T26418 pobj antibody,of
R7500 T26421 T26422 punct (,F3165
R7501 T26422 T26420 parataxis F3165,antibody
R7502 T26423 T26422 nmod SIGMA,F3165
R7503 T26424 T26422 punct ", ",F3165
R7504 T26425 T26422 punct ),F3165
R7505 T26426 T26417 cc and,μg
R7506 T26427 T26428 nummod 15,μl
R7507 T26428 T26417 conj μl,μg
R7508 T26429 T26428 prep of,μl
R7509 T26430 T26431 compound protein,Sepharose
R7510 T26431 T26429 pobj Sepharose,of
R7511 T26432 T26431 compound G,Sepharose
R7512 T26433 T26431 punct -,Sepharose
R7513 T26434 T26435 punct (,Amersham
R7514 T26435 T26431 parataxis Amersham,Sepharose
R7515 T26436 T26435 punct ),Amersham
R7516 T26437 T26405 prep on,mixed
R7517 T26438 T26439 det a,wheel
R7518 T26439 T26437 pobj wheel,on
R7519 T26440 T26439 amod rotating,wheel
R7520 T26441 T26405 prep at,mixed
R7521 T26442 T26443 nummod 4,°C
R7522 T26443 T26441 pobj °C,at
R7523 T26444 T26405 prep for,mixed
R7524 T26445 T26446 nummod 2,hrs
R7525 T26446 T26444 pobj hrs,for
R7526 T26447 T26405 punct .,mixed
R7527 T26449 T26450 compound Protein,concentration
R7528 T26450 T26451 nsubjpass concentration,determined
R7529 T26452 T26451 auxpass was,determined
R7530 T26453 T26451 prep by,determined
R7531 T26454 T26455 det the,system
R7532 T26455 T26453 pobj system,by
R7533 T26456 T26457 compound BCA,assay
R7534 T26457 T26455 compound assay,system
R7535 T26458 T26457 compound protein,assay
R7536 T26459 T26460 punct (,Pierce
R7537 T26460 T26455 parataxis Pierce,system
R7538 T26461 T26460 punct ),Pierce
R7539 T26462 T26451 punct .,determined
R7540 T26464 T26465 det The,beads
R7541 T26465 T26466 nsubjpass beads,washed
R7542 T26467 T26466 auxpass were,washed
R7543 T26468 T26466 advmod then,washed
R7544 T26469 T26470 nummod three,times
R7545 T26470 T26466 npadvmod times,washed
R7546 T26471 T26466 prep with,washed
R7547 T26472 T26473 compound immunoprecipitation,buffer
R7548 T26473 T26471 pobj buffer,with
R7549 T26474 T26466 cc and,washed
R7550 T26475 T26466 conj followed,washed
R7551 T26476 T26475 agent by,followed
R7552 T26477 T26478 nummod three,times
R7553 T26478 T26476 pobj times,by
R7554 T26479 T26478 prep with,times
R7555 T26480 T26481 compound wash,buffer
R7556 T26481 T26479 pobj buffer,with
R7557 T26482 T26483 punct (,HCl
R7558 T26483 T26481 parataxis HCl,buffer
R7559 T26484 T26485 nummod 50,mM
R7560 T26485 T26483 compound mM,HCl
R7561 T26486 T26483 compound Tris,HCl
R7562 T26487 T26483 punct -,HCl
R7563 T26488 T26489 punct [,pH
R7564 T26489 T26483 parataxis pH,HCl
R7565 T26490 T26489 nummod 7.5,pH
R7566 T26491 T26489 punct ],pH
R7567 T26492 T26483 punct ", ",HCl
R7568 T26493 T26494 nummod 150,mM
R7569 T26494 T26495 compound mM,NaCl
R7570 T26495 T26483 appos NaCl,HCl
R7571 T26496 T26483 punct ", ",HCl
R7572 T26497 T26498 nummod 20,mM
R7573 T26498 T26499 compound mM,MgCl2
R7574 T26499 T26483 appos MgCl2,HCl
R7575 T26500 T26483 punct ),HCl
R7576 T26501 T26466 punct .,washed
R7577 T26503 T26504 nsubjpass Proteins,boiled
R7578 T26505 T26504 auxpass were,boiled
R7579 T26506 T26504 advmod then,boiled
R7580 T26507 T26504 prep for,boiled
R7581 T26508 T26509 nummod 5,min
R7582 T26509 T26507 pobj min,for
R7583 T26510 T26504 prep in,boiled
R7584 T26511 T26512 compound SDS,buffer
R7585 T26512 T26510 pobj buffer,in
R7586 T26513 T26512 compound sample,buffer
R7587 T26514 T26515 punct (,SDS
R7588 T26515 T26512 parataxis SDS,buffer
R7589 T26516 T26517 nummod 1,%
R7590 T26517 T26515 compound %,SDS
R7591 T26518 T26515 punct ", ",SDS
R7592 T26519 T26520 nummod 1,mM
R7593 T26520 T26521 compound mM,Tris
R7594 T26521 T26515 appos Tris,SDS
R7595 T26522 T26523 punct [,pH
R7596 T26523 T26521 parataxis pH,Tris
R7597 T26524 T26523 nummod 6.8,pH
R7598 T26525 T26523 punct ],pH
R7599 T26526 T26515 punct ", ",SDS
R7600 T26527 T26528 nummod 40,mM
R7601 T26528 T26529 compound mM,DTT
R7602 T26529 T26515 appos DTT,SDS
R7603 T26530 T26515 punct ", ",SDS
R7604 T26531 T26532 nummod 4,%
R7605 T26532 T26533 compound %,glycerol
R7606 T26533 T26515 appos glycerol,SDS
R7607 T26534 T26515 punct ", ",SDS
R7608 T26535 T26536 nummod 0.01,%
R7609 T26536 T26537 compound %,Y
R7610 T26537 T26515 appos Y,SDS
R7611 T26538 T26537 compound pyronine,Y
R7612 T26539 T26515 punct ),SDS
R7613 T26540 T26504 punct .,boiled
R7614 T26542 T26543 det The,supernatants
R7615 T26543 T26544 nsubjpass supernatants,fractionated
R7616 T26545 T26544 auxpass were,fractionated
R7617 T26546 T26544 prep by,fractionated
R7618 T26547 T26548 nmod sodium,sulfate
R7619 T26548 T26550 nmod sulfate,gel
R7620 T26549 T26548 nmod dodecyl,sulfate
R7621 T26550 T26553 compound gel,electrophoresis
R7622 T26551 T26548 punct -,sulfate
R7623 T26552 T26548 appos polyacrylamide,sulfate
R7624 T26553 T26546 pobj electrophoresis,by
R7625 T26554 T26555 punct (,PAGE
R7626 T26555 T26553 parataxis PAGE,electrophoresis
R7627 T26556 T26555 compound SDS,PAGE
R7628 T26557 T26555 punct -,PAGE
R7629 T26558 T26555 punct ),PAGE
R7630 T26559 T26544 cc and,fractionated
R7631 T26560 T26544 conj transferred,fractionated
R7632 T26561 T26560 prep to,transferred
R7633 T26562 T26563 det the,membrane
R7634 T26563 T26561 pobj membrane,to
R7635 T26564 T26563 compound nitrocellulose,membrane
R7636 T26565 T26566 punct (,Medium
R7637 T26566 T26563 parataxis Medium,membrane
R7638 T26567 T26568 compound Trans,Blot
R7639 T26568 T26566 compound Blot,Medium
R7640 T26569 T26568 punct -,Blot
R7641 T26570 T26566 compound Transfer,Medium
R7642 T26571 T26566 punct ", ",Medium
R7643 T26572 T26573 compound Bio,Rad
R7644 T26573 T26566 appos Rad,Medium
R7645 T26574 T26573 punct -,Rad
R7646 T26575 T26566 punct ),Medium
R7647 T26576 T26544 punct .,fractionated
R7648 T26578 T26579 compound Western,blotting
R7649 T26579 T26580 nsubjpass blotting,performed
R7650 T26581 T26580 auxpass was,performed
R7651 T26582 T26580 prep with,performed
R7652 T26583 T26584 nmod rabbit,antibody
R7653 T26584 T26582 pobj antibody,with
R7654 T26585 T26584 amod polyclonal,antibody
R7655 T26586 T26584 amod anti-HA,antibody
R7656 T26587 T26588 punct (,Cruz
R7657 T26588 T26584 parataxis Cruz,antibody
R7658 T26589 T26588 compound Santa,Cruz
R7659 T26590 T26588 punct ", ",Cruz
R7660 T26591 T26592 punct #,sc
R7661 T26592 T26588 appos sc,Cruz
R7662 T26593 T26592 punct -,sc
R7663 T26594 T26592 nummod 805,sc
R7664 T26595 T26588 punct ),Cruz
R7665 T26596 T26580 punct .,performed
R7666 T26598 T26599 nsubjpass Signals,detected
R7667 T26600 T26599 auxpass were,detected
R7668 T26601 T26599 prep with,detected
R7669 T26602 T26603 compound horseradish,peroxidase
R7670 T26603 T26604 npadvmod peroxidase,conjugated
R7671 T26604 T26606 amod conjugated,IgG
R7672 T26605 T26604 punct -,conjugated
R7673 T26606 T26601 pobj IgG,with
R7674 T26607 T26606 nmod goat,IgG
R7675 T26608 T26606 amod anti-rabbit,IgG
R7676 T26609 T26606 cc and,IgG
R7677 T26610 T26611 nmod ECL,System
R7678 T26611 T26606 conj System,IgG
R7679 T26612 T26610 cc plus,ECL
R7680 T26613 T26614 compound Western,Blotting
R7681 T26614 T26610 conj Blotting,ECL
R7682 T26615 T26611 compound Detection,System
R7683 T26616 T26617 punct (,Amersham
R7684 T26617 T26611 parataxis Amersham,System
R7685 T26618 T26617 punct ),Amersham
R7686 T26619 T26599 punct .,detected
R7687 T27028 T27029 amod Subcellular,analysis
R7688 T27030 T27029 compound localization,analysis
R7689 T27032 T27033 nsubj We,transfected
R7690 T27034 T27035 det the,plasmid
R7691 T27035 T27033 dobj plasmid,transfected
R7692 T27036 T27035 acl encoding,plasmid
R7693 T27037 T27038 npadvmod HA,tagged
R7694 T27038 T27040 amod tagged,s
R7695 T27039 T27038 punct -,tagged
R7696 T27040 T27036 dobj s,encoding
R7697 T27041 T27042 amod full,length
R7698 T27042 T27040 compound length,s
R7699 T27043 T27042 punct -,length
R7700 T27044 T27040 compound mr,s
R7701 T27045 T27040 punct -,s
R7702 T27046 T27033 prep into,transfected
R7703 T27047 T27048 compound HEK293T,cells
R7704 T27048 T27046 pobj cells,into
R7705 T27049 T27033 punct ", ",transfected
R7706 T27050 T27033 conj seeded,transfected
R7707 T27051 T27050 dobj cells,seeded
R7708 T27052 T27050 prep on,seeded
R7709 T27053 T27052 pobj coverslips,on
R7710 T27054 T27053 acl coated,coverslips
R7711 T27055 T27054 prep with,coated
R7712 T27056 T27055 pobj collagen,with
R7713 T27057 T27058 nummod 48,hr
R7714 T27058 T27059 npadvmod hr,after
R7715 T27059 T27050 prep after,seeded
R7716 T27060 T27059 pobj transfection,after
R7717 T27061 T27050 punct ", ",seeded
R7718 T27062 T27050 conj fixed,seeded
R7719 T27063 T27062 prep with,fixed
R7720 T27064 T27065 nummod 4,%
R7721 T27065 T27066 compound %,paraformaldehyde
R7722 T27066 T27063 pobj paraformaldehyde,with
R7723 T27067 T27066 prep in,paraformaldehyde
R7724 T27068 T27069 npadvmod phosphate,buffered
R7725 T27069 T27071 amod buffered,saline
R7726 T27070 T27069 punct -,buffered
R7727 T27071 T27067 pobj saline,in
R7728 T27072 T27071 punct (,saline
R7729 T27073 T27071 appos PBS,saline
R7730 T27074 T27062 punct ),fixed
R7731 T27075 T27062 prep at,fixed
R7732 T27076 T27077 compound room,temperature
R7733 T27077 T27075 pobj temperature,at
R7734 T27078 T27062 prep for,fixed
R7735 T27079 T27080 nummod 20,min
R7736 T27080 T27078 pobj min,for
R7737 T27081 T27062 punct ", ",fixed
R7738 T27082 T27062 cc and,fixed
R7739 T27083 T27062 conj washed,fixed
R7740 T27084 T27083 prep with,washed
R7741 T27085 T27084 pobj PBS,with
R7742 T27086 T27033 punct .,transfected
R7743 T27088 T27089 advmod Then,permeabilized
R7744 T27090 T27089 nsubj we,permeabilized
R7745 T27091 T27089 dobj cells,permeabilized
R7746 T27092 T27089 prep in,permeabilized
R7747 T27093 T27092 pobj PBS,in
R7748 T27094 T27093 acl containing,PBS
R7749 T27095 T27096 nummod 0.1,%
R7750 T27096 T27097 compound %,100
R7751 T27097 T27094 dobj 100,containing
R7752 T27098 T27097 compound Triton,100
R7753 T27099 T27097 compound X,100
R7754 T27100 T27097 punct -,100
R7755 T27101 T27089 prep for,permeabilized
R7756 T27102 T27103 nummod 15,min
R7757 T27103 T27101 pobj min,for
R7758 T27104 T27089 punct ", ",permeabilized
R7759 T27105 T27089 conj washed,permeabilized
R7760 T27106 T27105 advmod again,washed
R7761 T27107 T27105 prep with,washed
R7762 T27108 T27107 pobj PBS,with
R7763 T27109 T27105 punct ", ",washed
R7764 T27110 T27105 cc and,washed
R7765 T27111 T27105 conj incubated,washed
R7766 T27112 T27111 prep in,incubated
R7767 T27113 T27112 pobj PBS,in
R7768 T27114 T27113 acl containing,PBS
R7769 T27115 T27116 nummod 0.1,%
R7770 T27116 T27114 dobj %,containing
R7771 T27117 T27118 amod bovine,albumin
R7772 T27118 T27116 appos albumin,%
R7773 T27119 T27118 compound serum,albumin
R7774 T27120 T27118 punct (,albumin
R7775 T27121 T27118 appos BSA,albumin
R7776 T27122 T27111 punct ),incubated
R7777 T27123 T27111 prep for,incubated
R7778 T27124 T27125 nummod 30,min
R7779 T27125 T27123 pobj min,for
R7780 T27126 T27111 prep at,incubated
R7781 T27127 T27128 compound room,temperature
R7782 T27128 T27126 pobj temperature,at
R7783 T27129 T27089 punct .,permeabilized
R7784 T27131 T27132 nsubj We,incubated
R7785 T27133 T27132 dobj cells,incubated
R7786 T27134 T27132 prep with,incubated
R7787 T27135 T27136 amod anti-HA,antibody
R7788 T27136 T27134 pobj antibody,with
R7789 T27137 T27132 advmod overnight,incubated
R7790 T27138 T27132 prep at,incubated
R7791 T27139 T27140 nummod 4,°C
R7792 T27140 T27138 pobj °C,at
R7793 T27141 T27142 punct (,1
R7794 T27142 T27132 parataxis 1,incubated
R7795 T27143 T27144 punct :,200
R7796 T27144 T27142 prep 200,1
R7797 T27145 T27142 prep in,1
R7798 T27146 T27145 pobj PBS,in
R7799 T27147 T27146 acl containing,PBS
R7800 T27148 T27149 nummod 0.2,%
R7801 T27149 T27150 compound %,BSA
R7802 T27150 T27147 dobj BSA,containing
R7803 T27151 T27142 punct ),1
R7804 T27152 T27132 punct .,incubated
R7805 T27154 T27155 det The,day
R7806 T27155 T27157 npadvmod day,washed
R7807 T27156 T27155 amod following,day
R7808 T27158 T27157 punct ", ",washed
R7809 T27159 T27157 nsubj we,washed
R7810 T27160 T27157 dobj cells,washed
R7811 T27161 T27162 nummod three,times
R7812 T27162 T27157 npadvmod times,washed
R7813 T27163 T27157 prep with,washed
R7814 T27164 T27163 pobj PBS,with
R7815 T27165 T27157 cc and,washed
R7816 T27166 T27157 conj incubated,washed
R7817 T27167 T27166 prep with,incubated
R7818 T27168 T27169 det a,IgG
R7819 T27169 T27167 pobj IgG,with
R7820 T27170 T27171 npadvmod Cy3,conjugated
R7821 T27171 T27169 amod conjugated,IgG
R7822 T27172 T27171 punct -,conjugated
R7823 T27173 T27169 compound goat,IgG
R7824 T27174 T27169 prep against,IgG
R7825 T27175 T27176 compound rabbit,IgG
R7826 T27176 T27174 pobj IgG,against
R7827 T27177 T27178 punct (,Laboratories
R7828 T27178 T27169 parataxis Laboratories,IgG
R7829 T27179 T27178 dep 1,Laboratories
R7830 T27180 T27181 punct :,400
R7831 T27181 T27179 prep 400,1
R7832 T27182 T27179 prep in,1
R7833 T27183 T27182 pobj PBS,in
R7834 T27184 T27178 punct ", ",Laboratories
R7835 T27185 T27178 compound Jackson,Laboratories
R7836 T27186 T27178 compound ImmunoReseach,Laboratories
R7837 T27187 T27178 punct ),Laboratories
R7838 T27188 T27166 prep for,incubated
R7839 T27189 T27190 nummod 30,min
R7840 T27190 T27188 pobj min,for
R7841 T27191 T27157 punct .,washed
R7842 T27193 T27194 nsubj We,rinsed
R7843 T27195 T27194 dobj cells,rinsed
R7844 T27196 T27197 nummod three,times
R7845 T27197 T27194 npadvmod times,rinsed
R7846 T27198 T27194 prep with,rinsed
R7847 T27199 T27198 pobj PBS,with
R7848 T27200 T27194 punct ", ",rinsed
R7849 T27201 T27194 advcl followed,rinsed
R7850 T27202 T27201 agent by,followed
R7851 T27203 T27202 pobj observation,by
R7852 T27204 T27203 acl using,observation
R7853 T27205 T27206 det a,FV300
R7854 T27206 T27204 dobj FV300,using
R7855 T27207 T27206 amod confocal,FV300
R7856 T27208 T27206 compound microscope,FV300
R7857 T27209 T27210 punct (,Olympus
R7858 T27210 T27206 parataxis Olympus,FV300
R7859 T27211 T27210 punct ),Olympus
R7860 T27212 T27206 acl equipped,FV300
R7861 T27213 T27212 prep with,equipped
R7862 T27214 T27215 det a,lens
R7863 T27215 T27213 pobj lens,with
R7864 T27216 T27215 nummod 60,lens
R7865 T27217 T27216 punct ×,60
R7866 T27218 T27215 compound objective,lens
R7867 T27219 T27194 punct .,rinsed
R7870 T27909 T27910 compound Luciferase,assay
R7871 T27912 T27913 prep For,constructed
R7872 T27914 T27915 amod transcriptional,analysis
R7873 T27915 T27912 pobj analysis,For
R7874 T27916 T27915 prep of,analysis
R7875 T27917 T27918 det the,region
R7876 T27918 T27916 pobj region,of
R7877 T27919 T27920 nummod 1.2,kb
R7878 T27920 T27918 compound kb,region
R7879 T27921 T27920 punct -,kb
R7880 T27922 T27918 compound promoter,region
R7881 T27923 T27918 prep of,region
R7882 T27924 T27925 compound mr,s
R7883 T27925 T27923 pobj s,of
R7884 T27926 T27925 punct -,s
R7885 T27927 T27913 punct ", ",constructed
R7886 T27928 T27913 nsubj we,constructed
R7887 T27929 T27930 det a,plasmid
R7888 T27930 T27913 dobj plasmid,constructed
R7889 T27931 T27930 compound reporter,plasmid
R7890 T27932 T27933 punct (,pro1.2k
R7891 T27933 T27930 parataxis pro1.2k,plasmid
R7892 T27934 T27933 punct ),pro1.2k
R7893 T27935 T27913 prep by,constructed
R7894 T27936 T27935 pcomp subcloning,by
R7895 T27937 T27938 det a,fragment
R7896 T27938 T27936 dobj fragment,subcloning
R7897 T27939 T27940 nummod 1.2,kb
R7898 T27940 T27938 nmod kb,fragment
R7899 T27941 T27940 punct -,kb
R7900 T27942 T27938 amod upstream,fragment
R7901 T27943 T27938 amod genomic,fragment
R7902 T27944 T27938 prep of,fragment
R7903 T27945 T27946 compound mr,s
R7904 T27946 T27948 compound s,gene
R7905 T27947 T27946 punct -,s
R7906 T27948 T27944 pobj gene,of
R7907 T27949 T27936 prep into,subcloning
R7908 T27950 T27951 det a,plasmid
R7909 T27951 T27949 pobj plasmid,into
R7910 T27952 T27953 compound pGL3,reporter
R7911 T27953 T27951 compound reporter,plasmid
R7912 T27954 T27953 compound luciferase,reporter
R7913 T27955 T27956 punct (,Promega
R7914 T27956 T27951 parataxis Promega,plasmid
R7915 T27957 T27956 punct ),Promega
R7916 T27958 T27913 punct .,constructed
R7917 T27960 T27961 det The,vectors
R7918 T27961 T27963 nsubjpass vectors,constructed
R7919 T27962 T27961 compound expression,vectors
R7920 T27964 T27963 auxpass were,constructed
R7921 T27965 T27963 prep by,constructed
R7922 T27966 T27965 pcomp subcloning,by
R7923 T27967 T27968 amod full,length
R7924 T27968 T27970 nmod length,genes
R7925 T27969 T27968 punct -,length
R7926 T27970 T27966 dobj genes,subcloning
R7927 T27971 T27972 nmod Crx,Nrl
R7928 T27972 T27970 compound Nrl,genes
R7929 T27973 T27972 punct ", ",Nrl
R7930 T27974 T27972 nmod Otx2,Nrl
R7931 T27975 T27972 punct ", ",Nrl
R7932 T27976 T27966 prep into,subcloning
R7933 T27977 T27978 det a,vector
R7934 T27978 T27976 pobj vector,into
R7935 T27979 T27978 compound pMIK,vector
R7936 T27980 T27978 compound expression,vector
R7937 T27981 T27982 punct (,gift
R7938 T27982 T27978 parataxis gift,vector
R7939 T27983 T27982 det a,gift
R7940 T27984 T27982 prep from,gift
R7941 T27985 T27986 compound Dr.,Maruyama
R7942 T27986 T27984 pobj Maruyama,from
R7943 T27987 T27986 compound K.,Maruyama
R7944 T27988 T27982 punct ),gift
R7945 T27989 T27966 punct ", ",subcloning
R7946 T27990 T27966 advmod respectively,subcloning
R7947 T27991 T27963 punct .,constructed
R7948 T27993 T27994 prep For,mutated
R7949 T27995 T27996 det the,vector
R7950 T27996 T27993 pobj vector,For
R7951 T27997 T27996 punct """",vector
R7952 T27998 T27996 nmod mut1259,vector
R7953 T27999 T27996 punct """",vector
R7954 T28000 T27994 punct ", ",mutated
R7955 T28001 T28002 det the,site
R7956 T28002 T27994 nsubjpass site,mutated
R7957 T28003 T28004 compound Crx,binding
R7958 T28004 T28002 compound binding,site
R7959 T28005 T28002 prep at,site
R7960 T28006 T28007 det the,position
R7961 T28007 T28005 pobj position,at
R7962 T28008 T28009 punct -,1259
R7963 T28009 T28010 nummod 1259,bp
R7964 T28010 T28007 compound bp,position
R7965 T28011 T27994 auxpass was,mutated
R7966 T28012 T27994 prep by,mutated
R7967 T28013 T28012 pcomp replacing,by
R7968 T28014 T28013 dobj GGATTA,replacing
R7969 T28015 T28013 prep with,replacing
R7970 T28016 T28015 pobj AGATCT,with
R7971 T28017 T27994 punct .,mutated
R7972 T28019 T28020 prep For,mutated
R7973 T28021 T28022 det the,vector
R7974 T28022 T28019 pobj vector,For
R7975 T28023 T28022 punct """",vector
R7976 T28024 T28022 nmod mut198,vector
R7977 T28025 T28022 punct """",vector
R7978 T28026 T28020 punct ", ",mutated
R7979 T28027 T28028 det the,site
R7980 T28028 T28020 nsubjpass site,mutated
R7981 T28029 T28030 compound Crx,binding
R7982 T28030 T28028 compound binding,site
R7983 T28031 T28028 prep at,site
R7984 T28032 T28033 det the,position
R7985 T28033 T28031 pobj position,at
R7986 T28034 T28035 punct -,198
R7987 T28035 T28036 nummod 198,bp
R7988 T28036 T28033 compound bp,position
R7989 T28037 T28020 auxpass was,mutated
R7990 T28038 T28020 prep by,mutated
R7991 T28039 T28038 pcomp replacing,by
R7992 T28040 T28039 dobj TAATCC,replacing
R7993 T28041 T28039 prep with,replacing
R7994 T28042 T28041 pobj GAATTC,with
R7995 T28043 T28020 punct .,mutated
R7996 T28045 T28046 prep For,mutated
R7997 T28047 T28048 det the,vector
R7998 T28048 T28045 pobj vector,For
R7999 T28049 T28048 punct """",vector
R8000 T28050 T28048 nmod mut72,vector
R8001 T28051 T28048 punct """",vector
R8002 T28052 T28046 punct ", ",mutated
R8003 T28053 T28054 det the,site
R8004 T28054 T28046 nsubjpass site,mutated
R8005 T28055 T28056 compound Crx,binding
R8006 T28056 T28054 compound binding,site
R8007 T28057 T28054 prep at,site
R8008 T28058 T28059 det the,position
R8009 T28059 T28057 pobj position,at
R8010 T28060 T28061 punct -,72
R8011 T28061 T28062 nummod 72,bp
R8012 T28062 T28059 compound bp,position
R8013 T28063 T28046 auxpass was,mutated
R8014 T28064 T28046 prep by,mutated
R8015 T28065 T28064 pcomp replacing,by
R8016 T28066 T28065 dobj GGATTA,replacing
R8017 T28067 T28065 prep with,replacing
R8018 T28068 T28067 pobj GAATTC,with
R8019 T28069 T28046 punct .,mutated
R8020 T28071 T28072 prep For,mutated
R8021 T28073 T28074 det the,vector
R8022 T28074 T28071 pobj vector,For
R8023 T28075 T28074 punct """",vector
R8024 T28076 T28077 nmod mut,all
R8025 T28077 T28074 nmod all,vector
R8026 T28078 T28074 punct """",vector
R8027 T28079 T28072 punct ", ",mutated
R8028 T28080 T28072 nsubjpass all,mutated
R8029 T28081 T28080 prep of,all
R8030 T28082 T28083 nummod three,sites
R8031 T28083 T28081 pobj sites,of
R8032 T28084 T28085 compound Crx,binding
R8033 T28085 T28083 compound binding,sites
R8034 T28086 T28072 auxpass were,mutated
R8035 T28087 T28072 punct .,mutated
R8036 T28089 T28090 compound 5xGAL4,pGL3
R8037 T28090 T28092 compound pGL3,plasmid
R8038 T28091 T28090 punct -,pGL3
R8039 T28092 T28095 nsubjpass plasmid,gifted
R8040 T28093 T28092 compound Control,plasmid
R8041 T28094 T28092 compound reporter,plasmid
R8042 T28096 T28092 punct ", ",plasmid
R8043 T28097 T28098 dep which,contains
R8044 T28098 T28092 relcl contains,plasmid
R8045 T28099 T28100 nummod five,copies
R8046 T28100 T28098 dobj copies,contains
R8047 T28101 T28100 prep of,copies
R8048 T28102 T28103 det the,sequence
R8049 T28103 T28101 pobj sequence,of
R8050 T28104 T28103 compound GAL4,sequence
R8051 T28105 T28106 compound DNA,recognition
R8052 T28106 T28103 compound recognition,sequence
R8053 T28107 T28100 acl positioned,copies
R8054 T28108 T28107 advmod immediately,positioned
R8055 T28109 T28108 advmod upstream,immediately
R8056 T28110 T28108 prep of,immediately
R8057 T28111 T28112 nmod SV40,promoter
R8058 T28112 T28110 pobj promoter,of
R8059 T28113 T28112 amod minimal,promoter
R8060 T28114 T28095 punct ", ",gifted
R8061 T28115 T28095 auxpass was,gifted
R8062 T28116 T28095 advmod kindly,gifted
R8063 T28117 T28095 agent from,gifted
R8064 T28118 T28119 compound Dr.,Noguchi
R8065 T28119 T28117 pobj Noguchi,from
R8066 T28120 T28119 compound T.,Noguchi
R8067 T28121 T28095 cc and,gifted
R8068 T28122 T28095 conj used,gifted
R8069 T28123 T28122 prep for,used
R8070 T28124 T28123 pobj analysis,for
R8071 T28125 T28124 prep of,analysis
R8072 T28126 T28127 det the,activity
R8073 T28127 T28125 pobj activity,of
R8074 T28128 T28127 amod transcriptional,activity
R8075 T28129 T28127 prep of,activity
R8076 T28130 T28131 compound mr,s
R8077 T28131 T28129 pobj s,of
R8078 T28132 T28131 punct -,s
R8079 T28133 T28134 punct [,50
R8080 T28134 T28122 parataxis 50,used
R8081 T28135 T28134 punct ],50
R8082 T28136 T28095 punct .,gifted
R8083 T28138 T28139 aux To,generate
R8084 T28139 T28140 advcl generate,produced
R8085 T28141 T28142 compound effector,plasmids
R8086 T28142 T28139 dobj plasmids,generate
R8087 T28143 T28140 punct ", ",produced
R8088 T28144 T28145 amod various,fragments
R8089 T28145 T28140 nsubj fragments,produced
R8090 T28146 T28145 compound deletion,fragments
R8091 T28147 T28140 aux were,produced
R8092 T28148 T28140 prep by,produced
R8093 T28149 T28150 compound PCR,reaction
R8094 T28150 T28148 pobj reaction,by
R8095 T28151 T28140 cc and,produced
R8096 T28152 T28140 conj subcloned,produced
R8097 T28153 T28152 prep into,subcloned
R8098 T28154 T28155 compound pGBKT7,vector
R8099 T28155 T28153 pobj vector,into
R8100 T28156 T28140 punct ", ",produced
R8101 T28157 T28140 advmod respectively,produced
R8102 T28158 T28140 punct .,produced
R8103 T28160 T28161 det These,fragments
R8104 T28161 T28162 nsubjpass fragments,digested
R8105 T28163 T28162 auxpass were,digested
R8106 T28164 T28162 prep with,digested
R8107 T28165 T28166 det the,sequence
R8108 T28166 T28164 pobj sequence,with
R8109 T28167 T28166 punct ", ",sequence
R8110 T28168 T28169 dep which,encodes
R8111 T28169 T28166 relcl encodes,sequence
R8112 T28170 T28171 compound GAL4,domain
R8113 T28171 T28169 dobj domain,encodes
R8114 T28172 T28173 compound DNA,binding
R8115 T28173 T28171 compound binding,domain
R8116 T28174 T28162 punct ", ",digested
R8117 T28175 T28162 cc and,digested
R8118 T28176 T28162 conj inserted,digested
R8119 T28177 T28176 prep into,inserted
R8120 T28178 T28177 pobj pcDNA3,into
R8121 T28179 T28162 punct .,digested
R8122 T28181 T28182 nsubj We,transfected
R8123 T28183 T28184 nummod 0.1,μg
R8124 T28184 T28182 dobj μg,transfected
R8125 T28185 T28184 prep of,μg
R8126 T28186 T28187 compound reporter,DNA
R8127 T28187 T28185 pobj DNA,of
R8128 T28188 T28187 compound plasmid,DNA
R8129 T28189 T28184 cc and,μg
R8130 T28190 T28191 nummod 2,μg
R8131 T28191 T28184 conj μg,μg
R8132 T28192 T28191 prep of,μg
R8133 T28193 T28194 det the,DNA
R8134 T28194 T28192 pobj DNA,of
R8135 T28195 T28196 compound expression,vector
R8136 T28196 T28194 compound vector,DNA
R8137 T28197 T28184 prep per,μg
R8138 T28198 T28199 nummod 6,cm
R8139 T28199 T28200 compound cm,dish
R8140 T28200 T28197 pobj dish,per
R8141 T28201 T28182 prep into,transfected
R8142 T28202 T28203 compound HEK293T,cells
R8143 T28203 T28201 pobj cells,into
R8144 T28204 T28182 advcl using,transfected
R8145 T28205 T28206 compound Fugene6,reagent
R8146 T28206 T28204 dobj reagent,using
R8147 T28207 T28206 compound transfection,reagent
R8148 T28208 T28182 punct .,transfected
R8149 T28210 T28211 nsubj We,analyzed
R8150 T28212 T28213 compound luciferase,activity
R8151 T28213 T28211 dobj activity,analyzed
R8152 T28214 T28215 nummod 48,hr
R8153 T28215 T28216 npadvmod hr,after
R8154 T28216 T28211 prep after,analyzed
R8155 T28217 T28216 pobj transfection,after
R8156 T28218 T28211 punct .,analyzed
R8157 T28701 T28702 nmod mr,s
R8158 T28702 T28704 nmod s,nucleotide
R8159 T28703 T28702 punct -,s
R8160 T28704 T28705 nmod nucleotide,sequences
R8161 T28706 T28704 cc and,nucleotide
R8162 T28707 T28708 compound amino,acid
R8163 T28708 T28704 conj acid,nucleotide
R8164 T28709 T28705 punct .,sequences
R8165 T28711 T28712 punct (,A
R8166 T28712 T28713 meta A,sequences
R8167 T28714 T28712 punct ),A
R8168 T28715 T28716 nmod mr,s
R8169 T28716 T28718 nmod s,nucleotide
R8170 T28717 T28716 punct -,s
R8171 T28718 T28713 nmod nucleotide,sequences
R8172 T28719 T28718 cc and,nucleotide
R8173 T28720 T28721 compound amino,acids
R8174 T28721 T28718 conj acids,nucleotide
R8175 T28722 T28713 punct .,sequences
R8176 T28724 T28725 amod Boxed,acids
R8177 T28725 T28727 nsubj acids,are
R8178 T28726 T28725 compound amino,acids
R8179 T28728 T28729 det the,sequence
R8180 T28729 T28727 attr sequence,are
R8181 T28730 T28731 compound SAM,domain
R8182 T28731 T28729 compound domain,sequence
R8183 T28732 T28727 cc and,are
R8184 T28733 T28734 det the,box
R8185 T28734 T28736 nsubj box,indicates
R8186 T28735 T28734 amod dashed,box
R8187 T28736 T28727 conj indicates,are
R8188 T28737 T28738 det a,signal
R8189 T28738 T28736 dobj signal,indicates
R8190 T28739 T28738 amod putative,signal
R8191 T28740 T28741 amod nuclear,localization
R8192 T28741 T28738 compound localization,signal
R8193 T28742 T28736 punct .,indicates
R8194 T28744 T28745 det The,underline
R8195 T28745 T28746 nsubj underline,indicates
R8196 T28747 T28748 det a,signal
R8197 T28748 T28746 dobj signal,indicates
R8198 T28749 T28748 amod putative,signal
R8199 T28750 T28751 compound polyadenylation,termination
R8200 T28751 T28748 compound termination,signal
R8201 T28752 T28746 punct .,indicates
R8202 T28754 T28755 punct (,B
R8203 T28755 T28756 meta B,Alignment
R8204 T28757 T28755 punct ),B
R8205 T28758 T28756 prep of,Alignment
R8206 T28759 T28760 compound SAM,domain
R8207 T28760 T28761 compound domain,sequences
R8208 T28761 T28758 pobj sequences,of
R8209 T28762 T28761 prep for,sequences
R8210 T28763 T28764 compound SAM,domain
R8211 T28764 T28765 npadvmod domain,containing
R8212 T28765 T28767 amod containing,proteins
R8213 T28766 T28765 punct -,containing
R8214 T28767 T28762 pobj proteins,for
R8215 T28768 T28756 punct .,Alignment
R8216 T28770 T28771 det The,helices
R8217 T28771 T28774 nsubjpass helices,marked
R8218 T28772 T28771 nummod five,helices
R8219 T28773 T28771 compound alpha,helices
R8220 T28775 T28774 auxpass are,marked
R8221 T28776 T28774 oprd H1,marked
R8222 T28777 T28778 punct -,H5
R8223 T28778 T28776 prep H5,H1
R8224 T28779 T28774 punct .,marked
R8225 T28781 T28782 amod Conserved,residues
R8226 T28782 T28785 nsubjpass residues,shown
R8227 T28783 T28784 compound amino,acid
R8228 T28784 T28782 compound acid,residues
R8229 T28786 T28785 auxpass are,shown
R8230 T28787 T28785 prep with,shown
R8231 T28788 T28789 det a,shadow
R8232 T28789 T28787 pobj shadow,with
R8233 T28790 T28789 amod dark,shadow
R8234 T28791 T28785 cc and,shown
R8235 T28792 T28793 advmod functionally,similar
R8236 T28793 T28794 amod similar,residues
R8237 T28794 T28795 nsubjpass residues,shown
R8238 T28795 T28785 conj shown,shown
R8239 T28796 T28795 auxpass are,shown
R8240 T28797 T28795 prep with,shown
R8241 T28798 T28799 det a,shadow
R8242 T28799 T28797 pobj shadow,with
R8243 T28800 T28799 amod light,shadow
R8244 T28801 T28795 punct .,shown
R8245 T28803 T28804 det The,sites
R8246 T28804 T28805 nsubjpass sites,indicated
R8247 T28806 T28807 dep that,targeted
R8248 T28807 T28804 relcl targeted,sites
R8249 T28808 T28807 auxpass were,targeted
R8250 T28809 T28807 prep for,targeted
R8251 T28810 T28809 pobj mutagenesis,for
R8252 T28811 T28805 auxpass are,indicated
R8253 T28812 T28805 prep by,indicated
R8254 T28813 T28812 pobj arrows,by
R8255 T28814 T28805 punct .,indicated
R8256 T28816 T28817 punct (,C
R8257 T28817 T28818 meta C,tree
R8258 T28819 T28817 punct ),C
R8259 T28820 T28818 amod Phylogenetic,tree
R8260 T28821 T28818 prep of,tree
R8261 T28822 T28823 compound SAM,domain
R8262 T28823 T28824 npadvmod domain,containing
R8263 T28824 T28826 amod containing,proteins
R8264 T28825 T28824 punct -,containing
R8265 T28826 T28821 pobj proteins,of
R8266 T28827 T28818 punct .,tree
R8267 T28829 T28830 compound Amino,acid
R8268 T28830 T28831 compound acid,sequences
R8269 T28831 T28832 nsubjpass sequences,analyzed
R8270 T28833 T28832 auxpass were,analyzed
R8271 T28834 T28832 prep by,analyzed
R8272 T28835 T28836 det the,method
R8273 T28836 T28834 pobj method,by
R8274 T28837 T28838 npadvmod neighbor,joining
R8275 T28838 T28836 amod joining,method
R8276 T28839 T28838 punct -,joining
R8277 T28840 T28832 prep in,analyzed
R8278 T28841 T28840 pobj MacVector,in
R8279 T28842 T28841 nummod 7.2,MacVector
R8280 T28843 T28832 punct .,analyzed
R8281 T28845 T28846 compound Branch,lengths
R8282 T28846 T28847 nsubj lengths,reflect
R8283 T28848 T28849 det the,number
R8284 T28849 T28847 dobj number,reflect
R8285 T28850 T28849 compound mean,number
R8286 T28851 T28849 prep of,number
R8287 T28852 T28851 pobj substitutions,of
R8288 T28853 T28852 prep per,substitutions
R8289 T28854 T28853 pobj site,per
R8290 T28855 T28847 punct .,reflect
R8291 T28857 T28858 punct (,D
R8292 T28858 T28859 meta D,comparison
R8293 T28860 T28858 punct ),D
R8294 T28861 T28859 amod Schematic,comparison
R8295 T28862 T28859 prep of,comparison
R8296 T28863 T28864 det the,sequences
R8297 T28864 T28862 pobj sequences,of
R8298 T28865 T28866 compound amino,acid
R8299 T28866 T28864 compound acid,sequences
R8300 T28867 T28864 prep for,sequences
R8301 T28868 T28869 nmod mouse,proteins
R8302 T28869 T28867 pobj proteins,for
R8303 T28870 T28868 punct ", ",mouse
R8304 T28871 T28868 conj rat,mouse
R8305 T28872 T28871 punct ", ",rat
R8306 T28873 T28871 amod human,rat
R8307 T28874 T28871 punct ", ",rat
R8308 T28875 T28871 conj chick,rat
R8309 T28876 T28875 cc and,chick
R8310 T28877 T28875 conj zebrafish,chick
R8311 T28878 T28879 compound mr,s
R8312 T28879 T28869 compound s,proteins
R8313 T28880 T28879 punct -,s
R8314 T28881 T28859 punct .,comparison
R8315 T28883 T28884 det The,similarity
R8316 T28884 T28886 nsubjpass similarity,shown
R8317 T28885 T28884 compound percent,similarity
R8318 T28887 T28884 prep of,similarity
R8319 T28888 T28889 det the,domains
R8320 T28889 T28887 pobj domains,of
R8321 T28890 T28889 compound SAM,domains
R8322 T28891 T28889 cc and,domains
R8323 T28892 T28893 amod other,regions
R8324 T28893 T28889 conj regions,domains
R8325 T28894 T28884 prep to,similarity
R8326 T28895 T28896 det the,regions
R8327 T28896 T28894 pobj regions,to
R8328 T28897 T28896 amod corresponding,regions
R8329 T28898 T28896 prep of,regions
R8330 T28899 T28900 det the,protein
R8331 T28900 T28898 pobj protein,of
R8332 T28901 T28900 compound mouse,protein
R8333 T28902 T28886 auxpass is,shown
R8334 T28903 T28886 punct .,shown
R8335 T28905 T28906 amod Overall,similarity
R8336 T28906 T28908 nsubjpass similarity,shown
R8337 T28907 T28906 compound sequence,similarity
R8338 T28909 T28906 prep with,similarity
R8339 T28910 T28911 det the,protein
R8340 T28911 T28909 pobj protein,with
R8341 T28912 T28911 compound mouse,protein
R8342 T28913 T28908 auxpass is,shown
R8343 T28914 T28908 prep on,shown
R8344 T28915 T28916 det the,right
R8345 T28916 T28914 pobj right,on
R8346 T28917 T28908 punct .,shown
R8351 T29458 T29459 compound mr,s
R8352 T29459 T29461 compound s,expression
R8353 T29460 T29459 punct -,s
R8354 T29462 T29461 prep in,expression
R8355 T29463 T29464 amod developing,retina
R8356 T29464 T29462 pobj retina,in
R8357 T29465 T29464 compound mouse,retina
R8358 T29466 T29464 cc and,retina
R8359 T29467 T29468 amod pineal,gland
R8360 T29468 T29464 conj gland,retina
R8361 T29469 T29461 punct .,expression
R8362 T29471 T29472 punct (,A
R8363 T29472 T29473 meta A,expression
R8364 T29474 T29472 punct -,A
R8365 T29475 T29472 dep G,A
R8366 T29476 T29472 punct ),A
R8367 T29477 T29478 compound mr,s
R8368 T29478 T29473 compound s,expression
R8369 T29479 T29478 punct -,s
R8370 T29480 T29473 prep during,expression
R8371 T29481 T29480 pobj development,during
R8372 T29482 T29481 prep of,development
R8373 T29483 T29484 det the,retina
R8374 T29484 T29482 pobj retina,of
R8375 T29485 T29484 compound mouse,retina
R8376 T29486 T29473 punct .,expression
R8377 T29488 T29489 det The,signal
R8378 T29489 T29493 nsubjpass signal,detected
R8379 T29490 T29491 advmod in,situ
R8380 T29491 T29489 amod situ,signal
R8381 T29492 T29489 compound hybridization,signal
R8382 T29494 T29489 prep of,signal
R8383 T29495 T29496 compound mr,s
R8384 T29496 T29494 pobj s,of
R8385 T29497 T29496 punct -,s
R8386 T29498 T29493 auxpass was,detected
R8387 T29499 T29493 neg not,detected
R8388 T29500 T29493 prep at,detected
R8389 T29501 T29500 pobj E13,at
R8390 T29502 T29503 punct (,A
R8391 T29503 T29493 parataxis A,detected
R8392 T29504 T29503 punct ),A
R8393 T29505 T29493 punct .,detected
R8394 T29507 T29508 det The,signal
R8395 T29508 T29509 nsubjpass signal,detected
R8396 T29510 T29511 punct (,arrow
R8397 T29511 T29508 parataxis arrow,signal
R8398 T29512 T29511 punct ),arrow
R8399 T29513 T29509 auxpass was,detected
R8400 T29514 T29509 advmod first,detected
R8401 T29515 T29509 prep in,detected
R8402 T29516 T29517 det the,aspect
R8403 T29517 T29515 pobj aspect,in
R8404 T29518 T29517 amod outer,aspect
R8405 T29519 T29517 prep of,aspect
R8406 T29520 T29519 pobj NBL,of
R8407 T29521 T29509 prep at,detected
R8408 T29522 T29521 pobj E18,at
R8409 T29523 T29524 punct (,B
R8410 T29524 T29509 parataxis B,detected
R8411 T29525 T29524 punct ),B
R8412 T29526 T29509 punct .,detected
R8413 T29528 T29529 det A,signal
R8414 T29529 T29534 nsubjpass signal,detected
R8415 T29530 T29529 amod strong,signal
R8416 T29531 T29532 compound mr,s
R8417 T29532 T29529 compound s,signal
R8418 T29533 T29532 punct -,s
R8419 T29535 T29534 auxpass was,detected
R8420 T29536 T29534 prep in,detected
R8421 T29537 T29538 amod outer,layer
R8422 T29538 T29536 pobj layer,in
R8423 T29539 T29538 prep of,layer
R8424 T29540 T29541 det the,retina
R8425 T29541 T29539 pobj retina,of
R8426 T29542 T29534 prep at,detected
R8427 T29543 T29542 pobj P3,at
R8428 T29544 T29545 punct -,P6
R8429 T29545 T29543 prep P6,P3
R8430 T29546 T29534 punct ", ",detected
R8431 T29547 T29534 cc and,detected
R8432 T29548 T29549 advmod then,decreased
R8433 T29549 T29534 conj decreased,detected
R8434 T29550 T29551 det the,signal
R8435 T29551 T29549 nsubj signal,decreased
R8436 T29552 T29549 prep in,decreased
R8437 T29553 T29554 det the,retina
R8438 T29554 T29552 pobj retina,in
R8439 T29555 T29554 amod adult,retina
R8440 T29556 T29557 punct (,E
R8441 T29557 T29549 parataxis E,decreased
R8442 T29558 T29559 punct -,G
R8443 T29559 T29557 prep G,E
R8444 T29560 T29557 punct ),E
R8445 T29561 T29549 punct .,decreased
R8446 T29563 T29564 nsubjpass Control,shown
R8447 T29565 T29563 prep with,Control
R8448 T29566 T29567 det the,probe
R8449 T29567 T29565 pobj probe,with
R8450 T29568 T29567 compound sense,probe
R8451 T29569 T29563 prep in,Control
R8452 T29570 T29571 compound E18,retina
R8453 T29571 T29569 pobj retina,in
R8454 T29572 T29564 auxpass is,shown
R8455 T29573 T29574 punct (,C
R8456 T29574 T29564 parataxis C,shown
R8457 T29575 T29574 punct ),C
R8458 T29576 T29564 punct .,shown
R8459 T29578 T29579 compound Scale,bar
R8460 T29580 T29579 punct ", ",bar
R8461 T29581 T29582 nummod 100,μm
R8462 T29582 T29579 appos μm,bar
R8463 T29583 T29579 punct .,bar
R8464 T29585 T29586 punct (,H
R8465 T29586 T29587 meta H,analysis
R8466 T29588 T29586 punct ),H
R8467 T29589 T29590 compound Northern,blot
R8468 T29590 T29587 compound blot,analysis
R8469 T29591 T29587 prep of,analysis
R8470 T29592 T29593 compound mr,s
R8471 T29593 T29595 compound s,expression
R8472 T29594 T29593 punct -,s
R8473 T29595 T29591 pobj expression,of
R8474 T29596 T29595 prep in,expression
R8475 T29597 T29598 amod adult,organs
R8476 T29598 T29596 pobj organs,in
R8477 T29599 T29598 compound mouse,organs
R8478 T29600 T29587 punct .,analysis
R8479 T29602 T29603 det The,arrow
R8480 T29603 T29604 nsubj arrow,corresponds
R8481 T29605 T29604 prep to,corresponds
R8482 T29606 T29607 compound 2.2kb,transcript
R8483 T29607 T29605 pobj transcript,to
R8484 T29608 T29609 compound mr,s
R8485 T29609 T29607 compound s,transcript
R8486 T29610 T29609 punct -,s
R8487 T29611 T29604 punct .,corresponds
R8488 T29613 T29614 punct (,I
R8489 T29614 T29615 meta I,analysis
R8490 T29616 T29614 punct ),I
R8491 T29617 T29618 compound RT,PCR
R8492 T29618 T29615 compound PCR,analysis
R8493 T29619 T29618 punct -,PCR
R8494 T29620 T29615 prep of,analysis
R8495 T29621 T29622 amod total,RNAs
R8496 T29622 T29620 pobj RNAs,of
R8497 T29623 T29622 acl extracted,RNAs
R8498 T29624 T29623 prep from,extracted
R8499 T29625 T29626 nmod E13,embryo
R8500 T29626 T29624 pobj embryo,from
R8501 T29627 T29626 amod whole,embryo
R8502 T29628 T29626 punct ", ",embryo
R8503 T29629 T29630 nmod P0,body
R8504 T29630 T29626 conj body,embryo
R8505 T29631 T29630 amod whole,body
R8506 T29632 T29630 punct (,body
R8507 T29633 T29630 prep except,body
R8508 T29634 T29633 prep for,except
R8509 T29635 T29636 det the,eye
R8510 T29636 T29634 pobj eye,for
R8511 T29637 T29630 punct ),body
R8512 T29638 T29630 punct ", ",body
R8513 T29639 T29640 compound P7,retina
R8514 T29640 T29630 conj retina,body
R8515 T29641 T29640 punct ", ",retina
R8516 T29642 T29643 nmod P7,gland
R8517 T29643 T29640 conj gland,retina
R8518 T29644 T29643 amod pineal,gland
R8519 T29645 T29643 punct ", ",gland
R8520 T29646 T29647 compound P7,brain
R8521 T29647 T29643 conj brain,gland
R8522 T29648 T29647 punct ", ",brain
R8523 T29649 T29650 compound P7,liver
R8524 T29650 T29647 conj liver,brain
R8525 T29651 T29650 punct ", ",liver
R8526 T29652 T29653 amod adult,retina
R8527 T29653 T29650 conj retina,liver
R8528 T29654 T29653 punct ", ",retina
R8529 T29655 T29656 amod adult,gland
R8530 T29656 T29653 conj gland,retina
R8531 T29657 T29656 amod pineal,gland
R8532 T29658 T29656 punct ", ",gland
R8533 T29659 T29660 amod adult,brain
R8534 T29660 T29656 conj brain,gland
R8535 T29661 T29660 cc and,brain
R8536 T29662 T29663 amod adult,liver
R8537 T29663 T29660 conj liver,brain
R8538 T29664 T29623 punct ", ",extracted
R8539 T29665 T29623 advmod respectively,extracted
R8540 T29666 T29615 punct .,analysis
R8541 T29669 T29668 punct ", ",RPE
R8542 T29670 T29671 amod retinal,epithelium
R8543 T29671 T29668 appos epithelium,RPE
R8544 T29672 T29671 compound pigment,epithelium
R8545 T29673 T29668 punct ;,RPE
R8546 T29674 T29668 appos NR,RPE
R8547 T29675 T29674 punct ", ",NR
R8548 T29676 T29677 amod neural,retina
R8549 T29677 T29674 appos retina,NR
R8550 T29678 T29668 punct ;,RPE
R8551 T29679 T29668 appos NBL,RPE
R8552 T29680 T29679 punct ", ",NBL
R8553 T29681 T29682 amod neuroblastic,layer
R8554 T29682 T29679 appos layer,NBL
R8555 T29683 T29668 punct ;,RPE
R8556 T29684 T29668 appos GCL,RPE
R8557 T29685 T29684 punct ", ",GCL
R8558 T29686 T29687 compound ganglion,cell
R8559 T29687 T29688 compound cell,layer
R8560 T29688 T29684 appos layer,GCL
R8561 T29689 T29668 punct ;,RPE
R8562 T29690 T29668 appos ONL,RPE
R8563 T29691 T29690 punct ", ",ONL
R8564 T29692 T29693 amod outer,layer
R8565 T29693 T29690 appos layer,ONL
R8566 T29694 T29693 amod nuclear,layer
R8567 T29695 T29668 punct ;,RPE
R8568 T29696 T29668 appos INL,RPE
R8569 T29697 T29696 punct ", ",INL
R8570 T29698 T29699 amod inner,layer
R8571 T29699 T29696 appos layer,INL
R8572 T29700 T29699 amod nuclear,layer
R8573 T29701 T29668 punct .,RPE
R8574 T30251 T30252 det The,transcription
R8575 T30252 T30253 nsubjpass transcription,regulated
R8576 T30254 T30252 prep of,transcription
R8577 T30255 T30256 compound mr,s
R8578 T30256 T30254 pobj s,of
R8579 T30257 T30256 punct -,s
R8580 T30258 T30253 auxpass is,regulated
R8581 T30259 T30253 agent by,regulated
R8582 T30260 T30259 pobj Crx,by
R8583 T30261 T30253 punct .,regulated
R8584 T30263 T30264 punct (,A
R8585 T30264 T30265 meta A,performed
R8586 T30266 T30264 punct ", ",A
R8587 T30267 T30264 dep B,A
R8588 T30268 T30264 punct ),A
R8589 T30269 T30270 advmod In,situ
R8590 T30270 T30271 amod situ,hybridization
R8591 T30271 T30265 nsubjpass hybridization,performed
R8592 T30272 T30271 acl using,hybridization
R8593 T30273 T30274 det a,probe
R8594 T30274 T30272 dobj probe,using
R8595 T30275 T30274 prep for,probe
R8596 T30276 T30277 compound mouse,s
R8597 T30277 T30275 pobj s,for
R8598 T30278 T30277 compound mr,s
R8599 T30279 T30277 punct -,s
R8600 T30280 T30265 auxpass was,performed
R8601 T30281 T30265 prep on,performed
R8602 T30282 T30283 det the,retinas
R8603 T30283 T30281 pobj retinas,on
R8604 T30284 T30285 amod wild,type
R8605 T30285 T30283 nmod type,retinas
R8606 T30286 T30285 punct -,type
R8607 T30287 T30288 punct (,A
R8608 T30288 T30285 parataxis A,type
R8609 T30289 T30288 punct ),A
R8610 T30290 T30285 cc and,type
R8611 T30291 T30292 compound Crx,KO
R8612 T30292 T30285 conj KO,type
R8613 T30293 T30294 punct (,B
R8614 T30294 T30283 parataxis B,retinas
R8615 T30295 T30294 punct ),B
R8616 T30296 T30265 prep at,performed
R8617 T30297 T30296 pobj P5,at
R8618 T30298 T30265 punct .,performed
R8619 T30300 T30301 compound mr,s
R8620 T30301 T30303 compound s,expression
R8621 T30302 T30301 punct -,s
R8622 T30303 T30304 nsubjpass expression,reduced
R8623 T30305 T30304 auxpass was,reduced
R8624 T30306 T30304 advmod drastically,reduced
R8625 T30307 T30304 prep in,reduced
R8626 T30308 T30309 det the,retina
R8627 T30309 T30307 pobj retina,in
R8628 T30310 T30311 compound Crx,KO
R8629 T30311 T30309 compound KO,retina
R8630 T30312 T30313 punct (,B
R8631 T30313 T30309 parataxis B,retina
R8632 T30314 T30313 punct ),B
R8633 T30315 T30304 punct .,reduced
R8634 T30317 T30318 compound Scale,bar
R8635 T30319 T30318 punct ", ",bar
R8636 T30320 T30321 nummod 100,μm
R8637 T30321 T30318 appos μm,bar
R8638 T30322 T30318 punct .,bar
R8639 T30324 T30325 punct (,C
R8640 T30325 T30326 meta C,analysis
R8641 T30327 T30325 punct ),C
R8642 T30328 T30329 compound RT,PCR
R8643 T30329 T30326 compound PCR,analysis
R8644 T30330 T30329 punct -,PCR
R8645 T30331 T30326 prep of,analysis
R8646 T30332 T30333 amod total,RNAs
R8647 T30333 T30331 pobj RNAs,of
R8648 T30334 T30333 acl extracted,RNAs
R8649 T30335 T30334 prep from,extracted
R8650 T30336 T30337 det the,glands
R8651 T30337 T30335 pobj glands,from
R8652 T30338 T30337 amod pineal,glands
R8653 T30339 T30337 prep of,glands
R8654 T30340 T30341 nmod P5,mouse
R8655 T30341 T30339 pobj mouse,of
R8656 T30342 T30343 amod wild,type
R8657 T30343 T30341 nmod type,mouse
R8658 T30344 T30343 punct -,type
R8659 T30345 T30343 cc and,type
R8660 T30346 T30347 compound Crx,KO
R8661 T30347 T30343 conj KO,type
R8662 T30348 T30326 punct .,analysis
R8663 T30350 T30351 det The,lanes
R8664 T30351 T30355 nsubj lanes,show
R8665 T30352 T30351 amod upper,lanes
R8666 T30353 T30352 cc and,upper
R8667 T30354 T30352 conj lower,upper
R8668 T30356 T30357 compound PCR,products
R8669 T30357 T30355 dobj products,show
R8670 T30358 T30357 acl amplified,products
R8671 T30359 T30358 agent by,amplified
R8672 T30360 T30361 det the,pairs
R8673 T30361 T30359 pobj pairs,by
R8674 T30362 T30361 compound primer,pairs
R8675 T30363 T30361 amod specific,pairs
R8676 T30364 T30363 prep for,specific
R8677 T30365 T30366 compound mr,s
R8678 T30366 T30364 pobj s,for
R8679 T30367 T30366 punct -,s
R8680 T30368 T30366 cc and,s
R8681 T30369 T30370 compound G3PDH,cDNAs
R8682 T30370 T30366 conj cDNAs,s
R8683 T30371 T30358 punct ", ",amplified
R8684 T30372 T30358 advmod respectively,amplified
R8685 T30373 T30355 punct .,show
R8686 T30375 T30376 nsubjpass Water,used
R8687 T30377 T30376 auxpass was,used
R8688 T30378 T30376 prep for,used
R8689 T30379 T30380 compound control,reaction
R8690 T30380 T30378 pobj reaction,for
R8691 T30381 T30382 compound RT,PCR
R8692 T30382 T30380 compound PCR,reaction
R8693 T30383 T30382 punct -,PCR
R8694 T30384 T30376 punct .,used
R8695 T30386 T30387 punct (,D
R8696 T30387 T30388 meta D,transactivates
R8697 T30389 T30387 punct -,D
R8698 T30390 T30387 dep F,D
R8699 T30391 T30387 punct ),D
R8700 T30392 T30388 nsubj Crx,transactivates
R8701 T30393 T30394 compound mr,s
R8702 T30394 T30396 compound s,transcription
R8703 T30395 T30394 punct -,s
R8704 T30396 T30388 dobj transcription,transactivates
R8705 T30397 T30388 punct .,transactivates
R8706 T30399 T30400 compound Reporter,plasmids
R8707 T30400 T30401 nsubjpass plasmids,shown
R8708 T30402 T30400 prep for,plasmids
R8709 T30403 T30404 det the,assay
R8710 T30404 T30402 pobj assay,for
R8711 T30405 T30404 compound luciferase,assay
R8712 T30406 T30401 auxpass are,shown
R8713 T30407 T30401 punct .,shown
R8714 T30409 T30410 amod Blue,boxes
R8715 T30410 T30411 nsubj boxes,represent
R8716 T30412 T30413 compound Crx,sites
R8717 T30413 T30411 dobj sites,represent
R8718 T30414 T30413 compound binding,sites
R8719 T30415 T30416 punct (,D
R8720 T30416 T30411 parataxis D,represent
R8721 T30417 T30416 punct ),D
R8722 T30418 T30411 punct .,represent
R8723 T30420 T30421 amod Relative,activity
R8724 T30421 T30422 nsubjpass activity,indicated
R8725 T30423 T30421 prep of,activity
R8726 T30424 T30425 det the,luciferase
R8727 T30425 T30423 pobj luciferase,of
R8728 T30426 T30422 auxpass is,indicated
R8729 T30427 T30428 advmod when,co-transfected
R8730 T30428 T30422 advcl co-transfected,indicated
R8731 T30429 T30428 nsubjpass Pro1.2k,co-transfected
R8732 T30430 T30428 auxpass was,co-transfected
R8733 T30431 T30428 prep with,co-transfected
R8734 T30432 T30431 pobj Crx,with
R8735 T30433 T30432 punct ", ",Crx
R8736 T30434 T30432 conj Otx2,Crx
R8737 T30435 T30434 punct ", ",Otx2
R8738 T30436 T30434 conj Nrl,Otx2
R8739 T30437 T30436 punct ", ",Nrl
R8740 T30438 T30436 cc and,Nrl
R8741 T30439 T30440 nmod Crx,Nrl
R8742 T30440 T30436 conj Nrl,Nrl
R8743 T30441 T30440 punct +,Nrl
R8744 T30442 T30428 punct ", ",co-transfected
R8745 T30443 T30428 advmod respectively,co-transfected
R8746 T30444 T30445 punct (,E
R8747 T30445 T30428 parataxis E,co-transfected
R8748 T30446 T30445 punct ),E
R8749 T30447 T30422 punct .,indicated
R8750 T30449 T30450 compound Fold,activation
R8751 T30450 T30451 nsubjpass activation,indicated
R8752 T30452 T30451 auxpass is,indicated
R8753 T30453 T30454 advmod when,co-transfected
R8754 T30454 T30451 advcl co-transfected,indicated
R8755 T30455 T30454 nsubjpass Pro1.2k,co-transfected
R8756 T30456 T30455 punct ", ",Pro1.2k
R8757 T30457 T30455 conj mut1259,Pro1.2k
R8758 T30458 T30457 punct ", ",mut1259
R8759 T30459 T30457 conj mut198,mut1259
R8760 T30460 T30459 punct ", ",mut198
R8761 T30461 T30459 conj mut72,mut198
R8762 T30462 T30461 cc and,mut72
R8763 T30463 T30464 compound mut,all
R8764 T30464 T30461 conj all,mut72
R8765 T30465 T30454 auxpass were,co-transfected
R8766 T30466 T30454 prep with,co-transfected
R8767 T30467 T30468 det the,vector
R8768 T30468 T30466 pobj vector,with
R8769 T30469 T30468 compound Crx,vector
R8770 T30470 T30468 compound expression,vector
R8771 T30471 T30472 punct (,Crx
R8772 T30472 T30468 parataxis Crx,vector
R8773 T30473 T30472 punct +,Crx
R8774 T30474 T30472 punct ),Crx
R8775 T30475 T30468 cc or,vector
R8776 T30476 T30477 det the,vector
R8777 T30477 T30468 conj vector,vector
R8778 T30478 T30477 amod empty,vector
R8779 T30479 T30480 punct (,Crx
R8780 T30480 T30477 parataxis Crx,vector
R8781 T30481 T30480 punct -,Crx
R8782 T30482 T30480 punct ),Crx
R8783 T30483 T30454 prep into,co-transfected
R8784 T30484 T30485 compound HEK293T,cells
R8785 T30485 T30483 pobj cells,into
R8786 T30486 T30487 punct (,F
R8787 T30487 T30451 parataxis F,indicated
R8788 T30488 T30487 punct ),F
R8789 T30489 T30451 punct .,indicated
R8790 T30491 T30492 compound Error,bars
R8791 T30492 T30493 nsubj bars,represent
R8792 T30494 T30495 amod standard,error
R8793 T30495 T30493 dobj error,represent
R8794 T30496 T30495 prep of,error
R8795 T30497 T30496 pobj mean,of
R8796 T30498 T30493 punct .,represent
R8797 T30893 T30892 prep of,Summary
R8798 T30894 T30895 nmod yeast,screening
R8799 T30895 T30893 pobj screening,of
R8800 T30896 T30897 nummod two,hybrid
R8801 T30897 T30895 compound hybrid,screening
R8802 T30898 T30897 punct -,hybrid
R8803 T30899 T30895 cc and,screening
R8804 T30900 T30901 compound GAL4,assay
R8805 T30901 T30895 conj assay,screening
R8806 T30902 T30892 punct .,Summary
R8807 T30904 T30905 punct (,A
R8808 T30905 T30906 meta A,indicated
R8809 T30907 T30905 punct ),A
R8810 T30908 T30909 amod Full,length
R8811 T30909 T30911 compound length,s
R8812 T30910 T30909 punct -,length
R8813 T30911 T30906 nsubjpass s,indicated
R8814 T30912 T30911 compound mr,s
R8815 T30913 T30911 punct -,s
R8816 T30914 T30911 prep as,s
R8817 T30915 T30916 det a,bait
R8818 T30916 T30914 pobj bait,as
R8819 T30917 T30916 acl used,bait
R8820 T30918 T30917 prep in,used
R8821 T30919 T30920 det the,screening
R8822 T30920 T30918 pobj screening,in
R8823 T30921 T30920 nmod yeast,screening
R8824 T30922 T30923 nummod two,hybrid
R8825 T30923 T30920 compound hybrid,screening
R8826 T30924 T30923 punct -,hybrid
R8827 T30925 T30911 cc and,s
R8828 T30926 T30927 amod positive,clones
R8829 T30927 T30911 conj clones,s
R8830 T30928 T30906 auxpass are,indicated
R8831 T30929 T30906 punct .,indicated
R8832 T30932 T30933 mark that,contain
R8833 T30933 T30931 ccomp contain,Note
R8834 T30934 T30933 nsubj all,contain
R8835 T30935 T30934 prep of,all
R8836 T30936 T30937 nummod five,clones
R8837 T30937 T30935 pobj clones,of
R8838 T30938 T30939 compound mr,s
R8839 T30939 T30937 compound s,clones
R8840 T30940 T30939 punct -,s
R8841 T30941 T30937 acl identified,clones
R8842 T30942 T30941 prep in,identified
R8843 T30943 T30944 det the,screening
R8844 T30944 T30942 pobj screening,in
R8845 T30945 T30946 compound SAM,domains
R8846 T30946 T30933 dobj domains,contain
R8847 T30947 T30931 punct .,Note
R8848 T30949 T30950 punct (,B
R8849 T30950 T30951 meta B,diagram
R8850 T30952 T30950 punct ),B
R8851 T30953 T30951 amod Schematic,diagram
R8852 T30954 T30951 prep of,diagram
R8853 T30955 T30956 det the,proteins
R8854 T30956 T30954 pobj proteins,of
R8855 T30957 T30958 compound mr,s
R8856 T30958 T30956 compound s,proteins
R8857 T30959 T30958 punct -,s
R8858 T30960 T30956 compound fusion,proteins
R8859 T30961 T30956 acl used,proteins
R8860 T30962 T30961 prep in,used
R8861 T30963 T30964 det the,assay
R8862 T30964 T30962 pobj assay,in
R8863 T30965 T30964 compound yeast,assay
R8864 T30966 T30964 compound GAL4,assay
R8865 T30967 T30951 punct .,diagram
R8866 T30969 T30970 amod Black,boxes
R8867 T30970 T30971 nsubj boxes,represent
R8868 T30972 T30973 det the,position
R8869 T30973 T30971 dobj position,represent
R8870 T30974 T30973 prep of,position
R8871 T30975 T30976 compound SAM,domains
R8872 T30976 T30974 pobj domains,of
R8873 T30977 T30971 punct .,represent
R8874 T30979 T30980 amod Relative,levels
R8875 T30980 T30981 nsubjpass levels,shown
R8876 T30982 T30980 prep of,levels
R8877 T30983 T30984 compound LacZ,expression
R8878 T30984 T30982 pobj expression,of
R8879 T30985 T30981 auxpass are,shown
R8880 T30986 T30981 prep on,shown
R8881 T30987 T30988 det the,right
R8882 T30988 T30986 pobj right,on
R8883 T30989 T30981 punct ", ",shown
R8884 T30990 T30981 advmod respectively,shown
R8885 T30991 T30981 punct .,shown
R8886 T30993 T30994 dep Note,indicates
R8887 T30995 T30994 punct ;,indicates
R8888 T30996 T30997 nsubj ++,indicates
R8889 T30997 T30994 ccomp indicates,indicates
R8890 T30998 T30999 det an,signal
R8891 T30999 T30997 dobj signal,indicates
R8892 T31000 T30999 amod intense,signal
R8893 T31001 T30999 amod blue,signal
R8894 T31002 T30999 relcl visible,signal
R8895 T31003 T31002 prep after,visible
R8896 T31004 T31003 pobj 12hr,after
R8897 T31005 T31004 prep of,12hr
R8898 T31006 T31005 pobj incubation,of
R8899 T31007 T31006 prep at,incubation
R8900 T31008 T31009 nummod 37,°C
R8901 T31009 T31007 pobj °C,at
R8902 T31010 T30994 punct ", ",indicates
R8903 T31011 T30994 nsubj +,indicates
R8904 T31012 T31013 det a,signal
R8905 T31013 T30994 dobj signal,indicates
R8906 T31014 T31013 amod blue,signal
R8907 T31015 T31013 relcl visible,signal
R8908 T31016 T31015 prep after,visible
R8909 T31017 T31016 pobj 24hr,after
R8910 T31018 T31017 prep of,24hr
R8911 T31019 T31018 pobj incubation,of
R8912 T31020 T30994 punct .,indicates
R8913 T31023 T31022 punct ", ",BD
R8914 T31024 T31025 compound binding,domain
R8915 T31025 T31022 appos domain,BD
R8916 T31026 T31022 punct ;,BD
R8917 T31027 T31022 appos AD,BD
R8918 T31028 T31027 punct ", ",AD
R8919 T31029 T31030 compound activation,domain
R8920 T31030 T31027 appos domain,AD
R8921 T31031 T31022 punct ;,BD
R8922 T31032 T31033 amod full,s
R8923 T31033 T31022 appos s,BD
R8924 T31034 T31033 punct ", ",s
R8925 T31035 T31036 amod full,length
R8926 T31036 T31033 compound length,s
R8927 T31037 T31036 punct -,length
R8928 T31038 T31033 compound mr,s
R8929 T31039 T31033 punct -,s
R8930 T31040 T31022 punct ;,BD
R8931 T31041 T31022 appos N,BD
R8932 T31042 T31041 punct ", ",N
R8933 T31043 T31044 npadvmod N,terminal
R8934 T31044 T31046 amod terminal,portion
R8935 T31045 T31044 punct -,terminal
R8936 T31046 T31041 appos portion,N
R8937 T31047 T31046 prep of,portion
R8938 T31048 T31049 compound mr,s
R8939 T31049 T31047 pobj s,of
R8940 T31050 T31049 punct -,s
R8941 T31051 T31052 punct (,1
R8942 T31052 T31046 parataxis 1,portion
R8943 T31053 T31052 nmod amino,1
R8944 T31054 T31052 nmod acids,1
R8945 T31055 T31052 prep to,1
R8946 T31056 T31055 pobj 400,to
R8947 T31057 T31052 punct ),1
R8948 T31058 T31022 punct ;,BD
R8949 T31059 T31022 appos C,BD
R8950 T31060 T31059 punct ", ",C
R8951 T31061 T31062 npadvmod C,terminal
R8952 T31062 T31064 amod terminal,portion
R8953 T31063 T31062 punct -,terminal
R8954 T31064 T31059 appos portion,C
R8955 T31065 T31064 prep of,portion
R8956 T31066 T31067 compound mr,s
R8957 T31067 T31065 pobj s,of
R8958 T31068 T31067 punct -,s
R8959 T31069 T31070 punct (,391
R8960 T31070 T31064 parataxis 391,portion
R8961 T31071 T31070 nmod amino,391
R8962 T31072 T31070 nmod acids,391
R8963 T31073 T31070 prep to,391
R8964 T31074 T31073 pobj 542,to
R8965 T31075 T31070 punct ),391
R8966 T31076 T31022 punct .,BD
R8967 T31407 T31408 det The,protein
R8968 T31408 T31412 nsubj protein,associate
R8969 T31409 T31410 compound mr,s
R8970 T31410 T31408 compound s,protein
R8971 T31411 T31410 punct -,s
R8972 T31413 T31412 aux can,associate
R8973 T31414 T31412 dep self,associate
R8974 T31415 T31412 punct -,associate
R8975 T31416 T31412 prep in,associate
R8976 T31417 T31418 amod mammalian,cells
R8977 T31418 T31416 pobj cells,in
R8978 T31419 T31412 punct .,associate
R8979 T31421 T31422 punct (,A
R8980 T31422 T31423 meta A,drawing
R8981 T31424 T31422 punct ),A
R8982 T31425 T31423 amod Schematic,drawing
R8983 T31426 T31423 prep of,drawing
R8984 T31427 T31428 det the,constructs
R8985 T31428 T31426 pobj constructs,of
R8986 T31429 T31428 acl used,constructs
R8987 T31430 T31429 prep for,used
R8988 T31431 T31432 compound immunoprecipitation,assay
R8989 T31432 T31430 pobj assay,for
R8990 T31433 T31423 punct .,drawing
R8991 T31435 T31436 npadvmod HA,tagged
R8992 T31436 T31438 amod tagged,regions
R8993 T31437 T31436 punct -,tagged
R8994 T31438 T31471 nsubjpass regions,inserted
R8995 T31439 T31436 cc or,tagged
R8996 T31440 T31441 npadvmod Flag,tagged
R8997 T31441 T31436 conj tagged,tagged
R8998 T31442 T31441 punct -,tagged
R8999 T31443 T31444 amod full,length
R9000 T31444 T31438 nmod length,regions
R9001 T31445 T31444 punct -,length
R9002 T31446 T31447 punct (,1
R9003 T31447 T31444 parataxis 1,length
R9004 T31448 T31447 nmod amino,1
R9005 T31449 T31447 nmod acids,1
R9006 T31450 T31447 prep to,1
R9007 T31451 T31450 pobj 542,to
R9008 T31452 T31447 punct ),1
R9009 T31453 T31438 punct ", ",regions
R9010 T31454 T31438 nmod ΔSAM,regions
R9011 T31455 T31456 punct (,1
R9012 T31456 T31454 parataxis 1,ΔSAM
R9013 T31457 T31456 nmod amino,1
R9014 T31458 T31456 nmod acids,1
R9015 T31459 T31456 prep to,1
R9016 T31460 T31459 pobj 400,to
R9017 T31461 T31456 punct ),1
R9018 T31462 T31454 cc and,ΔSAM
R9019 T31463 T31454 conj SAM,ΔSAM
R9020 T31464 T31465 punct (,401
R9021 T31465 T31463 parataxis 401,SAM
R9022 T31466 T31465 nmod amino,401
R9023 T31467 T31465 nmod acids,401
R9024 T31468 T31465 prep to,401
R9025 T31469 T31468 pobj 542,to
R9026 T31470 T31465 punct ),401
R9027 T31472 T31471 auxpass were,inserted
R9028 T31473 T31471 prep into,inserted
R9029 T31474 T31475 compound pcDNA3,vector
R9030 T31475 T31473 pobj vector,into
R9031 T31476 T31471 punct ", ",inserted
R9032 T31477 T31471 advmod respectively,inserted
R9033 T31478 T31471 punct .,inserted
R9034 T31480 T31481 punct (,B
R9035 T31481 T31482 meta B,transfected
R9036 T31483 T31481 punct ),B
R9037 T31484 T31485 det The,constructs
R9038 T31485 T31482 nsubjpass constructs,transfected
R9039 T31486 T31485 acl indicated,constructs
R9040 T31487 T31486 advmod above,indicated
R9041 T31488 T31482 auxpass were,transfected
R9042 T31489 T31482 prep into,transfected
R9043 T31490 T31491 compound HEK293T,cells
R9044 T31491 T31489 pobj cells,into
R9045 T31492 T31482 punct .,transfected
R9046 T31494 T31495 det Each,lane
R9047 T31495 T31496 nsubjpass lane,co-immunoprecipitated
R9048 T31497 T31496 auxpass was,co-immunoprecipitated
R9049 T31498 T31496 agent by,co-immunoprecipitated
R9050 T31499 T31500 amod anti-Flag,antibody
R9051 T31500 T31498 pobj antibody,by
R9052 T31501 T31496 cc and,co-immunoprecipitated
R9053 T31502 T31496 conj detected,co-immunoprecipitated
R9054 T31503 T31502 agent by,detected
R9055 T31504 T31505 amod anti-HA,antibody
R9056 T31505 T31503 pobj antibody,by
R9057 T31506 T31496 punct .,co-immunoprecipitated
R9058 T31508 T31509 compound Input,lysates
R9059 T31509 T31511 nsubjpass lysates,shown
R9060 T31510 T31509 compound protein,lysates
R9061 T31512 T31511 auxpass are,shown
R9062 T31513 T31511 prep in,shown
R9063 T31514 T31515 det the,panels
R9064 T31515 T31513 pobj panels,in
R9065 T31516 T31515 amod lower,panels
R9066 T31517 T31511 punct .,shown
R9067 T31519 T31520 punct (,C
R9068 T31520 T31521 meta C,generated
R9069 T31522 T31520 punct ),C
R9070 T31523 T31524 npadvmod Flag,tagged
R9071 T31524 T31526 amod tagged,mutants
R9072 T31525 T31524 punct -,tagged
R9073 T31526 T31521 nsubjpass mutants,generated
R9074 T31527 T31528 nummod two,site
R9075 T31528 T31529 npadvmod site,directed
R9076 T31529 T31526 amod directed,mutants
R9077 T31530 T31529 punct -,directed
R9078 T31531 T31532 compound mr,s
R9079 T31532 T31526 compound s,mutants
R9080 T31533 T31532 punct -,s
R9081 T31534 T31526 punct ", ",mutants
R9082 T31535 T31536 compound Flag,W404A
R9083 T31536 T31526 appos W404A,mutants
R9084 T31537 T31536 punct -,W404A
R9085 T31538 T31536 cc and,W404A
R9086 T31539 T31540 compound Flag,G453A
R9087 T31540 T31536 conj G453A,W404A
R9088 T31541 T31540 punct -,G453A
R9089 T31542 T31521 auxpass were,generated
R9090 T31543 T31521 cc and,generated
R9091 T31544 T31521 conj co-transfected,generated
R9092 T31545 T31544 prep with,co-transfected
R9093 T31546 T31547 amod full,HA
R9094 T31547 T31545 pobj HA,with
R9095 T31548 T31547 punct -,HA
R9096 T31549 T31521 punct .,generated
R9097 T31551 T31552 det Each,lane
R9098 T31552 T31553 nsubjpass lane,co-immunoprecipitated
R9099 T31554 T31553 auxpass was,co-immunoprecipitated
R9100 T31555 T31553 agent by,co-immunoprecipitated
R9101 T31556 T31557 amod anti-HA,antibody
R9102 T31557 T31555 pobj antibody,by
R9103 T31558 T31553 cc and,co-immunoprecipitated
R9104 T31559 T31553 conj detected,co-immunoprecipitated
R9105 T31560 T31559 agent by,detected
R9106 T31561 T31562 amod anti-Flag,antibody
R9107 T31562 T31560 pobj antibody,by
R9108 T31563 T31553 punct .,co-immunoprecipitated
R9109 T31729 T31730 amod Subcellular,localization
R9110 T31731 T31730 prep of,localization
R9111 T31732 T31733 compound mr,s
R9112 T31733 T31731 pobj s,of
R9113 T31734 T31733 punct -,s
R9114 T31735 T31730 prep in,localization
R9115 T31736 T31737 amod mammalian,cells
R9116 T31737 T31735 pobj cells,in
R9117 T31738 T31730 punct .,localization
R9118 T31740 T31741 punct (,A
R9119 T31741 T31742 meta A,transfected
R9120 T31743 T31741 punct ", ",A
R9121 T31744 T31741 dep B,A
R9122 T31745 T31741 punct ),A
R9123 T31746 T31747 npadvmod HA,tagged
R9124 T31747 T31749 amod tagged,s
R9125 T31748 T31747 punct -,tagged
R9126 T31749 T31742 nsubjpass s,transfected
R9127 T31750 T31751 amod full,length
R9128 T31751 T31749 compound length,s
R9129 T31752 T31751 punct -,length
R9130 T31753 T31749 compound mr,s
R9131 T31754 T31749 punct -,s
R9132 T31755 T31742 auxpass was,transfected
R9133 T31756 T31742 prep into,transfected
R9134 T31757 T31756 pobj HEK293T,into
R9135 T31758 T31759 punct (,B
R9136 T31759 T31742 parataxis B,transfected
R9137 T31760 T31759 punct ),B
R9138 T31761 T31742 cc and,transfected
R9139 T31762 T31742 conj detected,transfected
R9140 T31763 T31762 agent by,detected
R9141 T31764 T31765 amod anti-HA,antibody
R9142 T31765 T31763 pobj antibody,by
R9143 T31766 T31767 punct (,A
R9144 T31767 T31762 parataxis A,detected
R9145 T31768 T31767 punct ),A
R9146 T31769 T31742 punct .,transfected
R9147 T31771 T31772 compound Scale,bar
R9148 T31773 T31772 punct ", ",bar
R9149 T31774 T31775 nummod 20,μm
R9150 T31775 T31772 appos μm,bar
R9151 T31776 T31772 punct .,bar
R9152 T31778 T31779 punct (,C
R9153 T31779 T31780 meta C,cells
R9154 T31781 T31779 punct -,C
R9155 T31782 T31779 dep E,C
R9156 T31783 T31779 punct ),C
R9157 T31784 T31780 compound HEK293T,cells
R9158 T31785 T31780 acl immunostained,cells
R9159 T31786 T31785 prep with,immunostained
R9160 T31787 T31788 amod anti-HA,antibody
R9161 T31788 T31786 pobj antibody,with
R9162 T31789 T31790 punct (,C
R9163 T31790 T31780 parataxis C,cells
R9164 T31791 T31790 punct ),C
R9165 T31792 T31780 punct ", ",cells
R9166 T31793 T31780 conj DAPI,cells
R9167 T31794 T31795 punct (,D
R9168 T31795 T31793 parataxis D,DAPI
R9169 T31796 T31795 punct ),D
R9170 T31797 T31793 punct ", ",DAPI
R9171 T31798 T31793 cc and,DAPI
R9172 T31799 T31800 amod merged,image
R9173 T31800 T31793 conj image,DAPI
R9174 T31801 T31802 punct (,E
R9175 T31802 T31800 parataxis E,image
R9176 T31803 T31802 punct ),E
R9177 T31804 T31800 punct .,image
R9178 T31806 T31807 compound Scale,bar
R9179 T31808 T31807 punct ", ",bar
R9180 T31809 T31810 nummod 50,μm
R9181 T31810 T31807 appos μm,bar
R9182 T31811 T31807 punct .,bar
R9183 T32444 T32445 compound mr,s
R9184 T32445 T32447 nsubj s,functions
R9185 T32446 T32445 punct -,s
R9186 T32448 T32445 acl fused,s
R9187 T32449 T32448 prep to,fused
R9188 T32450 T32451 nmod GAL4,domain
R9189 T32451 T32449 pobj domain,to
R9190 T32452 T32453 npadvmod DNA,binding
R9191 T32453 T32451 amod binding,domain
R9192 T32454 T32447 prep as,functions
R9193 T32455 T32456 det a,repressor
R9194 T32456 T32454 pobj repressor,as
R9195 T32457 T32456 amod transcriptional,repressor
R9196 T32458 T32447 prep in,functions
R9197 T32459 T32460 compound HEK293T,cells
R9198 T32460 T32458 pobj cells,in
R9199 T32461 T32447 punct .,functions
R9200 T32463 T32464 punct (,A
R9201 T32464 T32465 meta A,drawing
R9202 T32466 T32464 punct ),A
R9203 T32467 T32465 amod Schematic,drawing
R9204 T32468 T32465 prep of,drawing
R9205 T32469 T32470 det the,constructs
R9206 T32470 T32468 pobj constructs,of
R9207 T32471 T32470 acl used,constructs
R9208 T32472 T32471 prep for,used
R9209 T32473 T32474 det the,assay
R9210 T32474 T32472 pobj assay,for
R9211 T32475 T32474 compound luciferase,assay
R9212 T32476 T32465 punct .,drawing
R9213 T32478 T32479 compound 5xGAL4,pGL3
R9214 T32479 T32481 compound pGL3,plasmid
R9215 T32480 T32479 punct -,pGL3
R9216 T32481 T32483 nsubjpass plasmid,co-transfected
R9217 T32482 T32481 compound reporter,plasmid
R9218 T32484 T32483 auxpass was,co-transfected
R9219 T32485 T32483 prep into,co-transfected
R9220 T32486 T32487 compound HEK293T,cells
R9221 T32487 T32485 pobj cells,into
R9222 T32488 T32487 prep with,cells
R9223 T32489 T32490 compound effector,plasmids
R9224 T32490 T32488 pobj plasmids,with
R9225 T32491 T32490 acl expressing,plasmids
R9226 T32492 T32493 amod various,mutants
R9227 T32493 T32491 dobj mutants,expressing
R9228 T32494 T32493 compound deletion,mutants
R9229 T32495 T32493 acl fused,mutants
R9230 T32496 T32495 prep to,fused
R9231 T32497 T32498 compound GAL4,DBD
R9232 T32498 T32496 pobj DBD,to
R9233 T32499 T32498 punct -,DBD
R9234 T32500 T32483 punct .,co-transfected
R9235 T32502 T32503 punct (,B
R9236 T32503 T32504 meta B,transfected
R9237 T32505 T32503 punct ),B
R9238 T32506 T32507 amod Various,amounts
R9239 T32507 T32504 nsubjpass amounts,transfected
R9240 T32508 T32507 prep of,amounts
R9241 T32509 T32510 nmod DBD,plasmids
R9242 T32510 T32508 pobj plasmids,of
R9243 T32511 T32509 punct ", ",DBD
R9244 T32512 T32513 compound DBD,mrs
R9245 T32513 T32509 conj mrs,DBD
R9246 T32514 T32513 punct -,mrs
R9247 T32515 T32513 punct ", ",mrs
R9248 T32516 T32517 compound DBD,N
R9249 T32517 T32513 conj N,mrs
R9250 T32518 T32517 punct -,N
R9251 T32519 T32517 cc or,N
R9252 T32520 T32521 compound DBD,C
R9253 T32521 T32517 conj C,N
R9254 T32522 T32521 punct -,C
R9255 T32523 T32504 auxpass were,transfected
R9256 T32524 T32504 prep with,transfected
R9257 T32525 T32526 nummod 0.1,μg
R9258 T32526 T32524 pobj μg,with
R9259 T32527 T32526 prep of,μg
R9260 T32528 T32529 compound 5xGAL4,pGL3
R9261 T32529 T32531 compound pGL3,plasmid
R9262 T32530 T32529 punct -,pGL3
R9263 T32531 T32527 pobj plasmid,of
R9264 T32532 T32531 compound reporter,plasmid
R9265 T32533 T32504 punct .,transfected
R9266 T32535 T32536 det The,activity
R9267 T32536 T32538 nsubjpass activity,designated
R9268 T32537 T32536 compound reporter,activity
R9269 T32539 T32536 prep in,activity
R9270 T32540 T32541 det the,presence
R9271 T32541 T32539 pobj presence,in
R9272 T32542 T32541 prep of,presence
R9273 T32543 T32544 det the,vector
R9274 T32544 T32542 pobj vector,of
R9275 T32545 T32544 compound pcDNA3,vector
R9276 T32546 T32547 punct (,pcDNA3
R9277 T32547 T32536 parataxis pcDNA3,activity
R9278 T32548 T32547 punct ),pcDNA3
R9279 T32549 T32538 auxpass was,designated
R9280 T32550 T32538 prep as,designated
R9281 T32551 T32550 pobj 1,as
R9282 T32552 T32538 punct .,designated
R9283 T32554 T32555 compound Error,bars
R9284 T32555 T32556 nsubj bars,represent
R9285 T32557 T32558 amod standard,error
R9286 T32558 T32556 dobj error,represent
R9287 T32559 T32558 prep of,error
R9288 T32560 T32559 pobj mean,of
R9289 T32561 T32556 punct .,represent
R9290 T32563 T32564 punct (,C
R9291 T32564 T32565 meta C,co-transfected
R9292 T32566 T32564 punct ),C
R9293 T32567 T32568 compound DBD,W404A
R9294 T32568 T32565 nsubjpass W404A,co-transfected
R9295 T32569 T32568 punct -,W404A
R9296 T32570 T32568 cc and,W404A
R9297 T32571 T32572 compound DBD,G453A
R9298 T32572 T32568 conj G453A,W404A
R9299 T32573 T32572 punct -,G453A
R9300 T32574 T32565 auxpass were,co-transfected
R9301 T32575 T32565 prep into,co-transfected
R9302 T32576 T32577 compound HEK293T,cells
R9303 T32577 T32575 pobj cells,into
R9304 T32578 T32565 prep with,co-transfected
R9305 T32579 T32580 compound 5xGAL4,pGL3
R9306 T32580 T32582 compound pGL3,plasmid
R9307 T32581 T32580 punct -,pGL3
R9308 T32582 T32578 pobj plasmid,with
R9309 T32583 T32582 compound reporter,plasmid
R9310 T32584 T32565 punct .,co-transfected
R9311 T32586 T32587 compound Fold,repression
R9312 T32587 T32588 nsubjpass repression,calculated
R9313 T32589 T32588 auxpass was,calculated
R9314 T32590 T32588 prep as,calculated
R9315 T32591 T32592 det the,decrease
R9316 T32592 T32590 pobj decrease,as
R9317 T32593 T32592 compound fold,decrease
R9318 T32594 T32592 prep in,decrease
R9319 T32595 T32596 compound luciferase,activity
R9320 T32596 T32594 pobj activity,in
R9321 T32597 T32592 prep compared,decrease
R9322 T32598 T32597 prep with,compared
R9323 T32599 T32600 compound DBD,mrs
R9324 T32600 T32598 pobj mrs,with
R9325 T32601 T32600 punct -,mrs
R9326 T32602 T32588 punct .,calculated
R9327 T32604 T32605 compound Error,bars
R9328 T32605 T32606 nsubj bars,represent
R9329 T32607 T32608 amod standard,deviation
R9330 T32608 T32606 dobj deviation,represent
R9331 T32609 T32606 punct .,represent
R9332 T32611 T32612 punct (,D
R9333 T32612 T32613 meta D,transfected
R9334 T32614 T32612 punct ),D
R9335 T32615 T32616 amod Various,amounts
R9336 T32616 T32613 nsubjpass amounts,transfected
R9337 T32617 T32616 prep of,amounts
R9338 T32618 T32619 compound DBD,tail
R9339 T32619 T32617 pobj tail,of
R9340 T32620 T32619 punct -,tail
R9341 T32621 T32619 cc or,tail
R9342 T32622 T32623 compound DBD,SAM
R9343 T32623 T32619 conj SAM,tail
R9344 T32624 T32623 punct -,SAM
R9345 T32625 T32613 auxpass were,transfected
R9346 T32626 T32613 prep with,transfected
R9347 T32627 T32628 compound 5xGAL4,pGL3
R9348 T32628 T32630 compound pGL3,plasmid
R9349 T32629 T32628 punct -,pGL3
R9350 T32630 T32626 pobj plasmid,with
R9351 T32631 T32630 compound reporter,plasmid
R9352 T32632 T32613 punct .,transfected
R9353 T32634 T32635 compound Error,bars
R9354 T32635 T32636 nsubj bars,represent
R9355 T32637 T32638 amod standard,error
R9356 T32638 T32636 dobj error,represent
R9357 T32639 T32638 prep of,error
R9358 T32640 T32639 pobj mean,of
R9359 T32641 T32636 punct .,represent
R9360 T32643 T32644 punct (,E
R9361 T32644 T32645 meta E,co-transfected
R9362 T32646 T32644 punct ),E
R9363 T32647 T32645 nsubjpass pcDNA3,co-transfected
R9364 T32648 T32647 cc or,pcDNA3
R9365 T32649 T32650 compound DBD,mrs
R9366 T32650 T32647 conj mrs,pcDNA3
R9367 T32651 T32650 punct -,mrs
R9368 T32652 T32653 punct (,μg
R9369 T32653 T32650 parataxis μg,mrs
R9370 T32654 T32653 nummod 5,μg
R9371 T32655 T32653 punct ),μg
R9372 T32656 T32645 auxpass was,co-transfected
R9373 T32657 T32645 prep into,co-transfected
R9374 T32658 T32659 compound Y79,cells
R9375 T32659 T32657 pobj cells,into
R9376 T32660 T32659 compound retinoblastoma,cells
R9377 T32661 T32645 prep with,co-transfected
R9378 T32662 T32663 nummod 0.5,μg
R9379 T32663 T32661 pobj μg,with
R9380 T32664 T32663 prep of,μg
R9381 T32665 T32666 compound 5xGAL4,pGL3
R9382 T32666 T32668 compound pGL3,plasmid
R9383 T32667 T32666 punct -,pGL3
R9384 T32668 T32664 pobj plasmid,of
R9385 T32669 T32668 compound reporter,plasmid
R9386 T32670 T32645 punct .,co-transfected
R9387 T32672 T32673 det The,activity
R9388 T32673 T32675 nsubjpass activity,designated
R9389 T32674 T32673 compound reporter,activity
R9390 T32676 T32673 prep in,activity
R9391 T32677 T32678 det the,presence
R9392 T32678 T32676 pobj presence,in
R9393 T32679 T32678 prep of,presence
R9394 T32680 T32679 pobj pcDNA3,of
R9395 T32681 T32675 auxpass was,designated
R9396 T32682 T32675 prep as,designated
R9397 T32683 T32682 pobj 1,as
R9398 T32684 T32675 punct .,designated
R9399 T32686 T32687 compound Error,bars
R9400 T32687 T32688 nsubj bars,represent
R9401 T32689 T32690 amod standard,deviation
R9402 T32690 T32688 dobj deviation,represent
R9403 T32691 T32688 punct .,represent
R9404 T32693 T32694 nsubj Asterisk,marks
R9405 T32695 T32696 advmod statistically,significant
R9406 T32696 T32697 amod significant,difference
R9407 T32697 T32694 dobj difference,marks
R9408 T32698 T32699 punct (,test
R9409 T32699 T32694 parataxis test,marks
R9410 T32700 T32699 poss Student,test
R9411 T32701 T32700 case 's,Student
R9412 T32702 T32699 compound t,test
R9413 T32703 T32699 punct : ,test
R9414 T32704 T32705 nsubj p,0.03
R9415 T32705 T32699 ccomp 0.03,test
R9416 T32706 T32705 punct <,0.03
R9417 T32707 T32699 punct ),test
R9418 T32708 T32694 punct .,marks
R9419 T32710 T32711 punct (,F
R9420 T32711 T32712 meta F,Alignment
R9421 T32713 T32711 punct ),F
R9422 T32714 T32712 prep of,Alignment
R9423 T32715 T32716 det the,regions
R9424 T32716 T32714 pobj regions,of
R9425 T32717 T32718 npadvmod C,terminal
R9426 T32718 T32716 amod terminal,regions
R9427 T32719 T32718 punct -,terminal
R9428 T32720 T32716 prep for,regions
R9429 T32721 T32722 nmod mouse,proteins
R9430 T32722 T32720 pobj proteins,for
R9431 T32723 T32721 punct ", ",mouse
R9432 T32724 T32721 conj rat,mouse
R9433 T32725 T32724 punct ", ",rat
R9434 T32726 T32724 amod human,rat
R9435 T32727 T32724 punct ", ",rat
R9436 T32728 T32724 conj chick,rat
R9437 T32729 T32728 cc and,chick
R9438 T32730 T32728 conj zebrafish,chick
R9439 T32731 T32732 compound mr,s
R9440 T32732 T32722 compound s,proteins
R9441 T32733 T32732 punct -,s
R9442 T32734 T32712 punct .,Alignment
R9443 T32736 T32737 amod Conserved,residues
R9444 T32737 T32740 nsubjpass residues,shown
R9445 T32738 T32739 compound amino,acid
R9446 T32739 T32737 compound acid,residues
R9447 T32741 T32740 auxpass are,shown
R9448 T32742 T32740 prep with,shown
R9449 T32743 T32744 det a,shadow
R9450 T32744 T32742 pobj shadow,with
R9451 T32745 T32744 amod dark,shadow
R9452 T32746 T32740 punct .,shown
R51 T1062 T1061 pobj proteins,of
R1287 T5570 T5554 dobj database,screened
R1 T1005 T1003 cc and,Cloning
R2 T1006 T1003 conj characterization,Cloning
R3 T1007 T1003 prep of,Cloning
R4 T1008 T1009 compound mr,s
R7 T1011 T1009 punct ", ",s
R8 T1012 T1013 det a,protein
R13 T1017 T1013 punct ", ",protein
R14 T1018 T1019 advmod predominantly,expressed
R15 T1019 T1013 acl expressed,protein
R16 T1020 T1019 prep in,expressed
R17 T1021 T1022 amod retinal,cells
R20 T1028 T1029 amod Sterile,motif
R27 T1036 T1037 punct ~,70
R28 T1037 T1038 nummod 70,residues
R29 T1038 T1039 npadvmod residues,long
R30 T1039 T1035 acomp long,are
R31 T1040 T1035 cc and,are
R32 T1041 T1042 aux have,reported
R35 T1044 T1042 prep as,reported
R36 T1045 T1046 amod common,modules
R39 T1048 T1050 compound protein,interaction
R42 T1051 T1035 punct .,are
R43 T1053 T1054 det This,domain
R44 T1054 T1055 nsubjpass domain,found
R45 T1056 T1055 auxpass is,found
R46 T1057 T1055 prep in,found
R47 T1058 T1059 det a,number
R50 T1061 T1059 prep of,number
R52 T1063 T1059 punct ", ",number
R53 T1064 T1059 prep including,number
R54 T1065 T1066 nmod Polycomb,group
R55 T1066 T1067 nmod group,proteins
R60 T1071 T1067 cc and,proteins
R61 T1072 T1073 compound ETS,family
R62 T1073 T1074 compound family,factors
R65 T1076 T1055 punct .,found
R66 T1078 T1079 prep In,report
R67 T1080 T1081 det this,work
R68 T1081 T1078 pobj work,In
R69 T1082 T1079 punct ", ",report
R70 T1083 T1079 nsubj we,report
R71 T1084 T1085 det the,cloning
R72 T1085 T1079 dobj cloning,report
R73 T1086 T1085 cc and,cloning
R74 T1087 T1088 amod functional,characterization
R75 T1088 T1085 conj characterization,cloning
R76 T1089 T1085 prep of,cloning
R77 T1090 T1091 det a,protein
R82 T1095 T1091 amod containing,protein
R84 T1097 T1091 punct ", ",protein
R85 T1098 T1099 dep which,expressed
R89 T1102 T1099 prep in,expressed
R90 T1103 T1104 amod retinal,photoreceptors
R91 T1104 T1102 pobj photoreceptors,in
R92 T1105 T1104 cc and,photoreceptors
R93 T1106 T1107 det the,gland
R96 T1109 T1099 cc and,expressed
R97 T1110 T1111 auxpass is,designated
R98 T1111 T1099 conj designated,expressed
R99 T1112 T1113 compound mouse,s
R103 T1116 T1117 punct (,protein
R109 T1122 T1117 punct ),protein
R110 T1123 T1079 punct .,report
R111 T1127 T1128 compound mr,s
R33 T1042 T1035 conj reported,are
R114 T1131 T1130 auxpass is,conserved
R115 T1132 T1130 advmod evolutionarily,conserved
R116 T1133 T1130 prep from,conserved
R117 T1134 T1133 pobj zebrafish,from
R118 T1135 T1133 prep through,from
R119 T1136 T1135 pobj human,through
R120 T1137 T1130 punct ", ",conserved
R121 T1138 T1130 npadvmod organisms,conserved
R122 T1139 T1140 prep through,conserved
R133 T1150 T1130 punct .,conserved
R134 T1152 T1153 amod Phylogenetic,analysis
R135 T1153 T1154 nsubj analysis,suggests
R136 T1155 T1156 mark that,related
R139 T1158 T1156 nsubjpass domain,related
R143 T1162 T1160 pobj s,of
R148 T1167 T1156 prep to,related
R149 T1168 T1169 det a,ortholog
R156 T1175 T1169 punct ", ",ortholog
R157 T1176 T1177 compound Mph1,Rae28
R160 T1179 T1169 punct ", ",ortholog
R161 T1180 T1181 dep which,known
R164 T1183 T1181 prep as,known
R165 T1184 T1185 det an,molecule
R168 T1187 T1185 acl involved,molecule
R169 T1188 T1187 prep in,involved
R170 T1189 T1190 compound chromatin,modifications
R171 T1190 T1188 pobj modifications,in
R172 T1191 T1154 punct .,suggests
R173 T1193 T1194 det These,findings
R174 T1194 T1195 nsubj findings,provide
R175 T1196 T1197 det the,possibility
R176 T1197 T1195 dobj possibility,provide
R177 T1198 T1199 mark that,play
R180 T1201 T1199 nsubj s,play
R183 T1204 T1205 det a,role
R186 T1207 T1199 prep by,play
R187 T1208 T1207 pcomp regulating,by
R188 T1209 T1210 compound gene,expression
R189 T1210 T1208 dobj expression,regulating
R190 T1211 T1208 prep in,regulating
R191 T1212 T1213 compound photoreceptor,development
R192 T1213 T1211 pobj development,in
R193 T1214 T1195 punct .,provide
R194 T1216 T1217 compound mr,s
R197 T1220 T1219 auxpass is,expressed
R198 T1221 T1219 advmod preferentially,expressed
R199 T1222 T1219 prep in,expressed
R200 T1223 T1224 det the,photoreceptors
R201 T1224 T1222 pobj photoreceptors,in
R202 T1225 T1219 prep at,expressed
R203 T1226 T1227 amod postnatal,3
R206 T1229 T1230 punct –,6
R207 T1230 T1227 prep 6,3
R208 T1231 T1232 punct (,P3
R209 T1232 T1227 parataxis P3,3
R210 T1233 T1234 punct -,6
R211 T1234 T1232 prep 6,P3
R212 T1235 T1232 punct ),P3
R213 T1236 T1227 punct ", ",3
R214 T1237 T1238 advmod when,undergo
R217 T1240 T1241 amod terminal,differentiation
R218 T1241 T1238 dobj differentiation,undergo
R219 T1242 T1219 punct ", ",expressed
R220 T1243 T1244 cc and,in
R221 T1244 T1219 prep in,expressed
R222 T1245 T1246 det the,gland
R226 T1249 T1219 punct .,expressed
R227 T1251 T1252 nsubjpass Transcription,regulated
R228 T1253 T1251 prep of,Transcription
R229 T1254 T1255 compound mr,s
R232 T1257 T1252 auxpass is,regulated
R233 T1258 T1252 advmod directly,regulated
R234 T1259 T1252 agent by,regulated
R235 T1260 T1261 det the,protein
R238 T1263 T1261 compound rod,protein
R241 T1266 T1261 appos Crx,protein
R242 T1267 T1252 punct .,regulated
R243 T1269 T1270 compound Immunoprecipitation,assay
R244 T1270 T1271 nsubj assay,showed
R245 T1272 T1273 mark that,associates
R254 T1281 T1282 advmod mainly,through
R255 T1282 T1273 prep through,associates
R256 T1283 T1284 det the,region
R260 T1287 T1284 amod containing,region
R262 T1289 T1290 advmod as,as
R265 T1292 T1284 conj ph,region
R266 T1293 T1271 punct .,showed
R267 T1295 T1296 det The,protein
R270 T1298 T1296 compound s,protein
R272 T1301 T1302 advmod mainly,in
R273 T1302 T1300 prep in,localizes
R274 T1303 T1304 det the,nucleus
R275 T1304 T1302 pobj nucleus,in
R276 T1305 T1300 punct ", ",localizes
R277 T1306 T1307 advmod when,overexpressed
R280 T1309 T1307 nsubjpass s,overexpressed
R283 T1312 T1307 prep in,overexpressed
R284 T1313 T1314 compound HEK293T,cells
R285 T1314 T1312 pobj cells,in
R286 T1315 T1300 punct .,localizes
R287 T1317 T1318 advmod Moreover,found
R288 T1319 T1318 punct ", ",found
R289 T1320 T1318 prep in,found
R290 T1321 T1322 det the,assays
R293 T1324 T1318 punct ", ",found
R294 T1325 T1318 nsubj we,found
R295 T1326 T1327 mark that,functions
R298 T1329 T1331 compound s,protein
R308 T1339 T1327 prep as,functions
R309 T1340 T1341 det a,repressor
R312 T1343 T1318 punct .,found
R313 T1345 T1346 nsubj We,revealed
R314 T1347 T1348 mark that,is
R317 T1350 T1348 nsubj activity,is
R321 T1354 T1352 pobj s,of
R323 T1356 T1348 neg not,is
R324 T1357 T1348 prep due,is
R325 T1358 T1357 prep to,due
R326 T1359 T1360 det a,interaction
R329 T1362 T1360 prep through,interaction
R330 T1363 T1364 poss its,domain
R333 T1366 T1358 cc but,to
R334 T1367 T1358 conj to,to
R335 T1368 T1369 det the,region
R338 T1371 T1369 amod terminal,region
R340 T1373 T1346 punct .,revealed
R341 T1377 T1378 nsubj We,identified
R342 T1379 T1380 det a,gene
R345 T1382 T1380 punct ", ",gene
R346 T1383 T1384 compound mr,s
R349 T1386 T1380 punct ", ",gene
R350 T1387 T1388 dep which,expressed
R354 T1391 T1388 prep in,expressed
R355 T1392 T1393 amod retinal,photoreceptors
R356 T1393 T1391 pobj photoreceptors,in
R357 T1394 T1393 cc and,photoreceptors
R358 T1395 T1396 amod pineal,gland
R359 T1396 T1393 conj gland,photoreceptors
R360 T1397 T1378 punct .,identified
R361 T1399 T1400 prep Based,predict
R362 T1401 T1399 prep on,Based
R363 T1402 T1403 poss its,pattern
R366 T1405 T1403 cc and,pattern
R367 T1406 T1407 amod biochemical,analysis
R368 T1407 T1403 conj analysis,pattern
R369 T1408 T1400 punct ", ",predict
R370 T1409 T1400 nsubj we,predict
R371 T1410 T1411 mark that,function
R374 T1413 T1411 nsubj s,function
R377 T1416 T1411 prep as,function
R378 T1417 T1418 det a,repressor
R381 T1420 T1411 prep in,function
R382 T1421 T1422 compound photoreceptor,cells
R383 T1422 T1420 pobj cells,in
R384 T1423 T1420 cc and,in
R385 T1424 T1420 conj in,in
R386 T1425 T1424 pobj pinealocytes,in
R387 T1426 T1425 prep of,pinealocytes
R388 T1427 T1428 det the,gland
R391 T1430 T1400 punct .,predict
R1903 T7585 T7586 det the,layer
R1904 T7586 T7584 pobj layer,to
R1905 T7587 T7586 amod outer,layer
R1906 T7588 T7586 amod nuclear,layer
R1907 T7589 T7586 punct (,layer
R1908 T7590 T7586 appos ONL,layer
R1909 T7591 T7583 punct ),localized
R1910 T7592 T7593 punct (,shown
R1911 T7593 T7583 parataxis shown,localized
R1912 T7594 T7593 nsubj data,shown
R1913 T7595 T7593 neg not,shown
R1914 T7596 T7593 punct ),shown
R1915 T7597 T7568 punct .,reduced
R1916 T7599 T7600 compound Faint,expression
R1917 T7600 T7601 nsubjpass expression,observed
R1918 T7602 T7600 prep of,expression
R1919 T7603 T7604 compound mr,s
R1920 T7604 T7606 compound s,mRNA
R1921 T7605 T7604 punct -,s
R1922 T7606 T7602 pobj mRNA,of
R1923 T7607 T7601 auxpass was,observed
R1924 T7608 T7601 prep in,observed
R1925 T7609 T7610 amod mature,photoreceptors
R1926 T7610 T7608 pobj photoreceptors,in
R1927 T7611 T7601 prep in,observed
R1928 T7612 T7613 det the,retina
R1929 T7613 T7611 pobj retina,in
R1930 T7614 T7613 amod adult,retina
R1931 T7615 T7616 punct (,2G
R1932 T7616 T7601 parataxis 2G,observed
R1933 T7617 T7616 compound Fig.,2G
R1934 T7618 T7616 punct ),2G
R1935 T7619 T7601 punct .,observed
R1936 T7628 T7630 nsubjpass in,e
R1937 T7632 T7633 det he,ti
R1938 T7634 T7630 conj s,e
R1939 T7635 T7634 nsubj s,s
R1940 T7637 T7636 amod u,e
R1941 T7640 T7639 compound s,pe
R1942 T7645 T7644 prep f,i
R1943 T7646 T7645 pobj i,f
R1944 T7652 T7651 prep of,y
R1945 T7654 T7653 pobj r,m
R1946 T7657 T7659 compound s,expression
R1947 T7658 T7657 punct -,s
R1948 T7660 T7656 punct ", ",examined
R1949 T7661 T7662 det the,expression
R1950 T7662 T7656 nsubjpass expression,examined
R1951 T7663 T7662 prep of,expression
R1952 T7664 T7665 det the,gene
R1953 T7665 T7663 pobj gene,of
R1954 T7666 T7667 compound mr,s
R1955 T7667 T7665 compound s,gene
R1956 T7668 T7667 punct -,s
R1957 T7669 T7662 prep in,expression
R1958 T7670 T7671 amod various,tissues
R1959 T7671 T7669 pobj tissues,in
R1960 T7672 T7671 amod adult,tissues
R1961 T7673 T7656 auxpass was,examined
R1962 T7674 T7656 prep by,examined
R1963 T7675 T7676 compound Northern,hybridization
R1964 T7676 T7674 pobj hybridization,by
R1965 T7677 T7678 punct (,2H
R1966 T7678 T7656 parataxis 2H,examined
R1967 T7679 T7678 compound Fig.,2H
R1968 T7680 T7678 punct ),2H
R1969 T7681 T7656 punct .,examined
R2404 T9889 T9887 dobj levels,compare
R2405 T9890 T9889 prep of,levels
R2406 T9891 T9892 compound mr,s
R2407 T9892 T9890 pobj s,of
R2408 T9893 T9892 punct -,s
R2409 T9894 T9887 prep in,compare
R2410 T9895 T9896 nmod P28,gland
R2411 T9896 T9894 pobj gland,in
R2412 T9897 T9898 amod wild,type
R2413 T9898 T9896 nmod type,gland
R2414 T9899 T9898 punct -,type
R2415 T9900 T9898 cc and,type
R2416 T9901 T9902 compound Crx,KO
R2417 T9902 T9898 conj KO,type
R2418 T9903 T9896 amod pineal,gland
R2419 T9904 T9905 punct (,3C
R2420 T9905 T9860 parataxis 3C,used
R2467 T9963 T9962 prep r,e
R2468 T9969 T9970 nsubjpass in,e
R2469 T9980 T9979 compound C,r
R2470 T9983 T9982 nsubj x,re
R2471 T9989 T9988 cc a,l
R2472 T9995 T9993 auxpass r,-s
R2473 T9996 T9993 prep t,-s
R2474 T9999 T10000 nsubj a,n
R2475 T10008 T10003 punct ", ",performed
R2476 T10009 T10003 nsubj we,performed
R2477 T10010 T10003 advmod next,performed
R2478 T10011 T10012 det a,assay
R2479 T10012 T10003 dobj assay,performed
R2480 T10013 T10012 compound luciferase,assay
R2481 T10014 T10003 advcl using,performed
R2482 T10015 T10016 det the,region
R2483 T10016 T10014 dobj region,using
R2484 T10017 T10018 nummod 1.2,kb
R2485 T10018 T10016 nmod kb,region
R2486 T10019 T10018 punct -,kb
R2487 T10020 T10016 amod proximal,region
R2488 T10021 T10016 compound promoter,region
R2489 T10022 T10016 prep of,region
R2490 T10023 T10024 compound mr,s
R2491 T10024 T10022 pobj s,of
R2492 T10025 T10024 punct -,s
R2493 T10026 T10016 acl fused,region
R2494 T10027 T10026 prep to,fused
R2495 T10028 T10029 det a,gene
R2496 T10029 T10027 pobj gene,to
R2497 T10030 T10029 compound luciferase,gene
R2498 T10031 T10029 prep as,gene
R2499 T10032 T10033 det a,reporter
R3293 T12919 T12917 ccomp was,indicated
R3297 T12923 T12921 relcl was,signal
R3305 T12931 T12923 acomp present,was
R3306 T12932 T12923 prep in,was
R3307 T12933 T12934 preconj both,contexts
R3308 T12934 T12932 pobj contexts,in
R3309 T12935 T12934 det the,contexts
R3310 T12936 T12934 nmod bait,contexts
R3311 T12937 T12936 cc and,bait
R3312 T12938 T12936 conj prey,bait
R3313 T12939 T12919 acomp weaker,was
R3314 T12940 T12939 prep than,weaker
R3315 T12941 T12942 advmod when,used
R3316 T12942 T12940 pcomp used,than
R3317 T12943 T12944 amod isolated,domains
R3318 T12944 T12942 nsubjpass domains,used
R3367 T12999 T13000 aux To,confirm
R3368 T13000 T13001 advcl confirm,performed
R3369 T13002 T13003 compound self,association
R3370 T13003 T13000 dobj association,confirm
R3371 T13004 T13003 punct -,association
R3529 T13236 T13234 pobj SAM,with
R3531 T13237 T13236 punct -,SAM
R3532 T13238 T13239 punct (,5B
R3534 T13239 T13228 parataxis 5B,co-immunoprecipitated
R3535 T13240 T13239 compound Fig.,5B
R3537 T13241 T13239 punct ", ",5B
R3538 T13242 T13239 appos lane,5B
R3539 T13243 T13242 nummod 10,lane
R3541 T13244 T13239 punct ),5B
R3542 T13245 T13202 punct .,co-immunoprecipitated
R3549 T13247 T13248 aux To,investigate
R3551 T13248 T13249 advcl investigate,generated
R3553 T13250 T13251 mark whether,associates
R3554 T13251 T13248 ccomp associates,investigate
R3556 T13252 T13253 det the,protein
R3557 T13253 T13251 nsubj protein,associates
R3558 T13254 T13255 compound mr,s
R3560 T13255 T13253 compound s,protein
R3561 T13256 T13255 punct -,s
R3563 T13257 T13251 dep self,associates
R3564 T13258 T13251 punct -,associates
R3565 T13259 T13251 advmod mainly,associates
R3567 T13260 T13251 prep through,associates
R3568 T13261 T13262 det the,domain
R3569 T13262 T13260 pobj domain,through
R3571 T13263 T13262 compound SAM,domain
R3572 T13264 T13249 punct ", ",generated
R3574 T13265 T13266 nummod two,mutations
R3575 T13266 T13249 nsubjpass mutations,generated
R3576 T13267 T13268 npadvmod site,directed
R3578 T13268 T13266 amod directed,mutations
R3579 T13269 T13268 punct -,directed
R3580 T13270 T13249 auxpass were,generated
R3582 T13271 T13249 prep in,generated
R3583 T13272 T13273 det the,domain
R3584 T13273 T13271 pobj domain,in
R3586 T13274 T13273 compound SAM,domain
R3587 T13275 T13273 prep of,domain
R3588 T13276 T13277 compound mr,s
R4766 T16914 T16911 ccomp functions,suggests
R4772 T16920 T16916 prep of,region
R4773 T16921 T16922 compound mouse,s
R4774 T16922 T16920 pobj s,of
R4775 T16923 T16922 compound mr,s
R4776 T16924 T16922 punct -,s
R4777 T16925 T16914 prep as,functions
R4778 T16926 T16927 det a,domain
R4779 T16927 T16925 pobj domain,as
R4780 T16928 T16927 amod transcriptional,domain
R4781 T16929 T16927 amod repressive,domain
R4782 T16930 T16927 prep in,domain
R4783 T16931 T16932 compound photoreceptor,development
R4784 T16932 T16930 pobj development,in
R4785 T16933 T16911 punct .,suggests
R4786 T16935 T16936 advmod However,contain
R4787 T16937 T16936 punct ", ",contain
R4788 T16938 T16939 det this,region
R4789 T16939 T16936 nsubj region,contain
R4790 T16940 T16936 aux does,contain
R4791 T16941 T16936 neg not,contain
R4792 T16942 T16936 amod characteristic,contain
R4793 T16943 T16944 compound amino,acid
R4794 T16944 T16936 dobj acid,contain
R4795 T16945 T16936 dep motifs,contain
R4796 T16946 T16936 cc and,contain
R4797 T16947 T16948 det the,mechanism
R4798 T16948 T16949 nsubj mechanism,remains
R4799 T16949 T16936 conj remains,contain
R4800 T16950 T16951 prep through,achieves
R4801 T16951 T16948 relcl achieves,mechanism
R4802 T16952 T16950 pobj which,through
R4803 T16953 T16954 det the,region
R4804 T16954 T16951 nsubj region,achieves
R4818 T16969 T16970 advcl Taken,suggest
R4819 T16971 T16969 advmod together,Taken
R4820 T16972 T16970 punct ", ",suggest
R4821 T16973 T16974 poss our,findings
R4822 T16974 T16970 nsubj findings,suggest
R4823 T16975 T16976 mark that,functions
R754 T3986 T3984 pobj mouse,in
R755 T3987 T3970 cc and,begin
R758 T3989 T3991 nsubj upregulation,is
R1118 T4369 T4367 dobj state,stabilize
R1237 T4501 T4482 punct .,found
R1238 T4503 T4504 det These,findings
R6388 T22335 T22333 prep of,function
R6391 T22336 T22337 compound mr,s
R6392 T22337 T22335 pobj s,of
R6393 T22319 T22317 xcomp regulate,thought
R6395 T22338 T22337 punct -,s
R6397 T22322 T22323 nmod cone,genes
R6398 T22323 T22319 dobj genes,regulate
R6399 T22339 T22331 punct ", ",be
R6400 T22324 T22325 npadvmod photoreceptor,specific
R6401 T22325 T22323 amod specific,genes
R6402 T22340 T22331 nsubj analysis,be
R6403 T22326 T22325 punct -,specific
R6404 T22327 T22283 punct .,suggest
R6405 T22341 T22340 prep of,analysis
R6406 T22329 T22330 aux To,clarify
R6407 T22330 T22331 advcl clarify,be
R6408 T22342 T22341 pobj mice,of
R6409 T22332 T22333 det the,function
R6410 T22343 T22342 prep with,mice
R6411 T22333 T22330 dobj function,clarify
R6412 T22334 T22333 amod biological,function
R6413 T22344 T22345 amod targeted,disruptions
R6414 T22345 T22343 pobj disruptions,with
R6415 T22346 T22345 prep of,disruptions
R6416 T22347 T22348 compound mr,s
R6417 T22348 T22346 pobj s,of
R6418 T22349 T22348 punct -,s
R6419 T22350 T22331 aux will,be
R6420 T22351 T22352 advmod very,important
R6421 T22352 T22331 acomp important,be
R6422 T22353 T22331 punct .,be