Id |
Subject |
Object |
Predicate |
Lexical cue |
T3287 |
347-349 |
VB |
denotes |
be |
T3221 |
0-4 |
NNS |
denotes |
Mice |
T3222 |
60-69 |
VBN |
denotes |
generated |
T3223 |
5-13 |
VBG |
denotes |
carrying |
T3224 |
14-15 |
DT |
denotes |
a |
T3225 |
25-33 |
NN |
denotes |
mutation |
T3226 |
16-24 |
VBN |
denotes |
targeted |
T3227 |
34-36 |
IN |
denotes |
in |
T3228 |
37-42 |
PRP$ |
denotes |
their |
T3229 |
50-54 |
NN |
denotes |
gene |
T3230 |
43-49 |
NN |
denotes |
Adam11 |
T3231 |
55-59 |
VBD |
denotes |
were |
T3232 |
70-72 |
IN |
denotes |
by |
T3233 |
73-83 |
JJ |
denotes |
homologous |
T3234 |
84-97 |
NN |
denotes |
recombination |
T3235 |
98-99 |
-LRB- |
denotes |
( |
T3236 |
104-106 |
NN |
denotes |
1A |
T3237 |
99-103 |
NN |
denotes |
Fig. |
T3238 |
106-107 |
-RRB- |
denotes |
) |
T3239 |
107-108 |
. |
denotes |
. |
T3240 |
108-185 |
sentence |
denotes |
Correct targeting events were confirmed by Southern blot analysis (Fig. 1B). |
T3241 |
109-116 |
JJ |
denotes |
Correct |
T3242 |
127-133 |
NNS |
denotes |
events |
T3243 |
117-126 |
NN |
denotes |
targeting |
T3244 |
139-148 |
VBN |
denotes |
confirmed |
T3245 |
134-138 |
VBD |
denotes |
were |
T3246 |
149-151 |
IN |
denotes |
by |
T3247 |
152-160 |
NNP |
denotes |
Southern |
T3248 |
161-165 |
NN |
denotes |
blot |
T3249 |
166-174 |
NN |
denotes |
analysis |
T3250 |
175-176 |
-LRB- |
denotes |
( |
T3251 |
181-183 |
NN |
denotes |
1B |
T3252 |
176-180 |
NN |
denotes |
Fig. |
T3253 |
183-184 |
-RRB- |
denotes |
) |
T3254 |
184-185 |
. |
denotes |
. |
T3255 |
185-388 |
sentence |
denotes |
As a result of this procedure, since a termination codon was introduced in exon 5 in the pro-protein domain, only the truncated form of the ADAM11 protein would be synthesised from this targeted allele. |
T3256 |
186-188 |
IN |
denotes |
As |
T3257 |
350-361 |
VBN |
denotes |
synthesised |
T3258 |
189-190 |
DT |
denotes |
a |
T3259 |
191-197 |
NN |
denotes |
result |
T3260 |
198-200 |
IN |
denotes |
of |
T3261 |
201-205 |
DT |
denotes |
this |
T3262 |
206-215 |
NN |
denotes |
procedure |
T3263 |
215-217 |
, |
denotes |
, |
T3264 |
217-222 |
IN |
denotes |
since |
T3265 |
247-257 |
VBN |
denotes |
introduced |
T3266 |
223-224 |
DT |
denotes |
a |
T3267 |
237-242 |
NN |
denotes |
codon |
T3268 |
225-236 |
NN |
denotes |
termination |
T3269 |
243-246 |
VBD |
denotes |
was |
T3270 |
258-260 |
IN |
denotes |
in |
T3271 |
261-265 |
NN |
denotes |
exon |
T3272 |
266-267 |
CD |
denotes |
5 |
T3273 |
268-270 |
IN |
denotes |
in |
T3274 |
271-274 |
DT |
denotes |
the |
T3275 |
287-293 |
NN |
denotes |
domain |
T3276 |
275-286 |
JJ |
denotes |
pro-protein |
T3277 |
293-295 |
, |
denotes |
, |
T3278 |
295-299 |
RB |
denotes |
only |
T3279 |
314-318 |
NN |
denotes |
form |
T3280 |
300-303 |
DT |
denotes |
the |
T3281 |
304-313 |
VBN |
denotes |
truncated |
T3282 |
319-321 |
IN |
denotes |
of |
T3283 |
322-325 |
DT |
denotes |
the |
T3284 |
333-340 |
NN |
denotes |
protein |
T3285 |
326-332 |
NN |
denotes |
ADAM11 |
T3286 |
341-346 |
MD |
denotes |
would |
T3288 |
362-366 |
IN |
denotes |
from |
T3289 |
367-371 |
DT |
denotes |
this |
T3290 |
381-387 |
NN |
denotes |
allele |
T3291 |
372-380 |
VBN |
denotes |
targeted |
T3292 |
387-388 |
. |
denotes |
. |
T3293 |
388-622 |
sentence |
denotes |
Because pro-protein domains are always removed in mature functional ADAM-proteins by furin-like proteases and are thus thought to be non-functional, we concluded that this truncated form of ADAM11 protein would have no function [26]. |
T3294 |
389-396 |
IN |
denotes |
Because |
T3295 |
428-435 |
VBN |
denotes |
removed |
T3296 |
397-408 |
JJ |
denotes |
pro-protein |
T3297 |
409-416 |
NNS |
denotes |
domains |
T3298 |
417-420 |
VBP |
denotes |
are |
T3299 |
421-427 |
RB |
denotes |
always |
T3300 |
541-550 |
VBD |
denotes |
concluded |
T3301 |
436-438 |
IN |
denotes |
in |
T3302 |
439-445 |
JJ |
denotes |
mature |
T3303 |
462-470 |
NN |
denotes |
proteins |
T3304 |
446-456 |
JJ |
denotes |
functional |
T3305 |
457-461 |
NN |
denotes |
ADAM |
T3306 |
461-462 |
HYPH |
denotes |
- |
T3307 |
471-473 |
IN |
denotes |
by |
T3308 |
474-479 |
NN |
denotes |
furin |
T3309 |
480-484 |
JJ |
denotes |
like |
T3310 |
479-480 |
HYPH |
denotes |
- |
T3311 |
485-494 |
NNS |
denotes |
proteases |
T3312 |
495-498 |
CC |
denotes |
and |
T3313 |
499-502 |
VBP |
denotes |
are |
T3314 |
508-515 |
VBN |
denotes |
thought |
T3315 |
503-507 |
RB |
denotes |
thus |
T3316 |
516-518 |
TO |
denotes |
to |
T3317 |
519-521 |
VB |
denotes |
be |
T3318 |
522-536 |
JJ |
denotes |
non-functional |
T3319 |
536-538 |
, |
denotes |
, |
T3320 |
538-540 |
PRP |
denotes |
we |
T3321 |
551-555 |
IN |
denotes |
that |
T3322 |
600-604 |
VB |
denotes |
have |
T3323 |
556-560 |
DT |
denotes |
this |
T3324 |
571-575 |
NN |
denotes |
form |
T3325 |
561-570 |
VBN |
denotes |
truncated |
T3326 |
576-578 |
IN |
denotes |
of |
T3327 |
579-585 |
NN |
denotes |
ADAM11 |
T3328 |
586-593 |
NN |
denotes |
protein |
T3329 |
594-599 |
MD |
denotes |
would |
T3330 |
605-607 |
DT |
denotes |
no |
T3331 |
608-616 |
NN |
denotes |
function |
T3332 |
617-618 |
-LRB- |
denotes |
[ |
T3333 |
618-620 |
CD |
denotes |
26 |
T3334 |
620-621 |
-RRB- |
denotes |
] |
T3335 |
621-622 |
. |
denotes |
. |
T3336 |
622-788 |
sentence |
denotes |
Absence of mature ADAM11 protein in homozygous mutants was confirmed by Western blot analysis using a specific antibody that recognises the ADAM11 protein (Fig. 1C). |
T3337 |
623-630 |
NN |
denotes |
Absence |
T3338 |
682-691 |
VBN |
denotes |
confirmed |
T3339 |
631-633 |
IN |
denotes |
of |
T3340 |
634-640 |
JJ |
denotes |
mature |
T3341 |
648-655 |
NN |
denotes |
protein |
T3342 |
641-647 |
NN |
denotes |
ADAM11 |
T3343 |
656-658 |
IN |
denotes |
in |
T3344 |
659-669 |
JJ |
denotes |
homozygous |
T3345 |
670-677 |
NNS |
denotes |
mutants |
T3346 |
678-681 |
VBD |
denotes |
was |
T3347 |
692-694 |
IN |
denotes |
by |
T3348 |
695-702 |
NNP |
denotes |
Western |
T3349 |
703-707 |
NN |
denotes |
blot |
T3350 |
708-716 |
NN |
denotes |
analysis |
T3351 |
717-722 |
VBG |
denotes |
using |
T3352 |
723-724 |
DT |
denotes |
a |
T3353 |
734-742 |
NN |
denotes |
antibody |
T3354 |
725-733 |
JJ |
denotes |
specific |
T3355 |
743-747 |
WDT |
denotes |
that |
T3356 |
748-758 |
VBZ |
denotes |
recognises |
T3357 |
759-762 |
DT |
denotes |
the |
T3358 |
770-777 |
NN |
denotes |
protein |
T3359 |
763-769 |
NN |
denotes |
ADAM11 |
T3360 |
778-779 |
-LRB- |
denotes |
( |
T3361 |
784-786 |
NN |
denotes |
1C |
T3362 |
779-783 |
NN |
denotes |
Fig. |
T3363 |
786-787 |
-RRB- |
denotes |
) |
T3364 |
787-788 |
. |
denotes |
. |
T3365 |
788-917 |
sentence |
denotes |
Analysis by Western blot showed that heterozygous mutants produced approximately half of the wild-type levels of ADAM11 protein. |
T3366 |
789-797 |
NN |
denotes |
Analysis |
T3367 |
814-820 |
VBD |
denotes |
showed |
T3368 |
798-800 |
IN |
denotes |
by |
T3369 |
801-808 |
NNP |
denotes |
Western |
T3370 |
809-813 |
NN |
denotes |
blot |
T3371 |
821-825 |
IN |
denotes |
that |
T3372 |
847-855 |
VBD |
denotes |
produced |
T3373 |
826-838 |
JJ |
denotes |
heterozygous |
T3374 |
839-846 |
NNS |
denotes |
mutants |
T3375 |
856-869 |
RB |
denotes |
approximately |
T3376 |
870-874 |
NN |
denotes |
half |
T3377 |
875-877 |
IN |
denotes |
of |
T3378 |
878-881 |
DT |
denotes |
the |
T3379 |
892-898 |
NNS |
denotes |
levels |
T3380 |
882-886 |
JJ |
denotes |
wild |
T3381 |
887-891 |
NN |
denotes |
type |
T3382 |
886-887 |
HYPH |
denotes |
- |
T3383 |
899-901 |
IN |
denotes |
of |
T3384 |
902-908 |
NN |
denotes |
ADAM11 |
T3385 |
909-916 |
NN |
denotes |
protein |
T3386 |
916-917 |
. |
denotes |
. |
R806 |
T3221 |
T3222 |
nsubjpass |
Mice,generated |
R807 |
T3223 |
T3221 |
acl |
carrying,Mice |
R808 |
T3224 |
T3225 |
det |
a,mutation |
R809 |
T3225 |
T3223 |
dobj |
mutation,carrying |
R810 |
T3226 |
T3225 |
amod |
targeted,mutation |
R811 |
T3227 |
T3223 |
prep |
in,carrying |
R812 |
T3228 |
T3229 |
poss |
their,gene |
R813 |
T3229 |
T3227 |
pobj |
gene,in |
R814 |
T3230 |
T3229 |
compound |
Adam11,gene |
R815 |
T3231 |
T3222 |
auxpass |
were,generated |
R816 |
T3232 |
T3222 |
prep |
by,generated |
R817 |
T3233 |
T3234 |
amod |
homologous,recombination |
R818 |
T3234 |
T3232 |
pobj |
recombination,by |
R819 |
T3235 |
T3236 |
punct |
(,1A |
R820 |
T3236 |
T3222 |
parataxis |
1A,generated |
R821 |
T3237 |
T3236 |
compound |
Fig.,1A |
R822 |
T3238 |
T3236 |
punct |
),1A |
R823 |
T3239 |
T3222 |
punct |
.,generated |
R824 |
T3241 |
T3242 |
amod |
Correct,events |
R825 |
T3242 |
T3244 |
nsubjpass |
events,confirmed |
R826 |
T3243 |
T3242 |
compound |
targeting,events |
R827 |
T3245 |
T3244 |
auxpass |
were,confirmed |
R828 |
T3246 |
T3244 |
agent |
by,confirmed |
R829 |
T3247 |
T3248 |
compound |
Southern,blot |
R830 |
T3248 |
T3249 |
compound |
blot,analysis |
R831 |
T3249 |
T3246 |
pobj |
analysis,by |
R832 |
T3250 |
T3251 |
punct |
(,1B |
R833 |
T3251 |
T3244 |
parataxis |
1B,confirmed |
R834 |
T3252 |
T3251 |
compound |
Fig.,1B |
R835 |
T3253 |
T3251 |
punct |
),1B |
R836 |
T3254 |
T3244 |
punct |
.,confirmed |
R837 |
T3256 |
T3257 |
prep |
As,synthesised |
R838 |
T3258 |
T3259 |
det |
a,result |
R839 |
T3259 |
T3256 |
pobj |
result,As |
R840 |
T3260 |
T3259 |
prep |
of,result |
R841 |
T3261 |
T3262 |
det |
this,procedure |
R842 |
T3262 |
T3260 |
pobj |
procedure,of |
R843 |
T3263 |
T3257 |
punct |
", ",synthesised |
R844 |
T3264 |
T3265 |
mark |
since,introduced |
R846 |
T3266 |
T3267 |
det |
a,codon |
R847 |
T3267 |
T3265 |
nsubjpass |
codon,introduced |
R848 |
T3268 |
T3267 |
compound |
termination,codon |
R849 |
T3269 |
T3265 |
auxpass |
was,introduced |
R850 |
T3270 |
T3265 |
prep |
in,introduced |
R851 |
T3271 |
T3270 |
pobj |
exon,in |
R852 |
T3272 |
T3271 |
nummod |
5,exon |
R853 |
T3273 |
T3265 |
prep |
in,introduced |
R854 |
T3274 |
T3275 |
det |
the,domain |
R855 |
T3275 |
T3273 |
pobj |
domain,in |
R856 |
T3276 |
T3275 |
amod |
pro-protein,domain |
R857 |
T3277 |
T3257 |
punct |
", ",synthesised |
R858 |
T3278 |
T3279 |
advmod |
only,form |
R859 |
T3279 |
T3257 |
nsubjpass |
form,synthesised |
R860 |
T3280 |
T3279 |
det |
the,form |
R861 |
T3281 |
T3279 |
amod |
truncated,form |
R862 |
T3282 |
T3279 |
prep |
of,form |
R863 |
T3283 |
T3284 |
det |
the,protein |
R864 |
T3284 |
T3282 |
pobj |
protein,of |
R865 |
T3285 |
T3284 |
compound |
ADAM11,protein |
R866 |
T3286 |
T3257 |
aux |
would,synthesised |
R867 |
T3287 |
T3257 |
auxpass |
be,synthesised |
R868 |
T3288 |
T3257 |
prep |
from,synthesised |
R869 |
T3289 |
T3290 |
det |
this,allele |
R870 |
T3290 |
T3288 |
pobj |
allele,from |
R871 |
T3291 |
T3290 |
amod |
targeted,allele |
R872 |
T3292 |
T3257 |
punct |
.,synthesised |
R873 |
T3294 |
T3295 |
mark |
Because,removed |
R875 |
T3296 |
T3297 |
amod |
pro-protein,domains |
R876 |
T3297 |
T3295 |
nsubjpass |
domains,removed |
R877 |
T3298 |
T3295 |
auxpass |
are,removed |
R878 |
T3299 |
T3295 |
advmod |
always,removed |
R879 |
T3301 |
T3295 |
prep |
in,removed |
R880 |
T3302 |
T3303 |
amod |
mature,proteins |
R881 |
T3303 |
T3301 |
pobj |
proteins,in |
R882 |
T3304 |
T3303 |
amod |
functional,proteins |
R883 |
T3305 |
T3303 |
compound |
ADAM,proteins |
R884 |
T3306 |
T3303 |
punct |
-,proteins |
R885 |
T3307 |
T3295 |
agent |
by,removed |
R886 |
T3308 |
T3309 |
npadvmod |
furin,like |
R887 |
T3309 |
T3311 |
amod |
like,proteases |
R888 |
T3310 |
T3309 |
punct |
-,like |
R889 |
T3311 |
T3307 |
pobj |
proteases,by |
R890 |
T3312 |
T3295 |
cc |
and,removed |
R891 |
T3313 |
T3314 |
auxpass |
are,thought |
R892 |
T3314 |
T3295 |
conj |
thought,removed |
R893 |
T3315 |
T3314 |
advmod |
thus,thought |
R894 |
T3316 |
T3317 |
aux |
to,be |
R895 |
T3317 |
T3314 |
xcomp |
be,thought |
R896 |
T3318 |
T3317 |
acomp |
non-functional,be |
R897 |
T3319 |
T3300 |
punct |
", ",concluded |
R898 |
T3320 |
T3300 |
nsubj |
we,concluded |
R899 |
T3321 |
T3322 |
mark |
that,have |
R900 |
T3322 |
T3300 |
ccomp |
have,concluded |
R901 |
T3323 |
T3324 |
det |
this,form |
R902 |
T3324 |
T3322 |
nsubj |
form,have |
R903 |
T3325 |
T3324 |
amod |
truncated,form |
R904 |
T3326 |
T3324 |
prep |
of,form |
R905 |
T3327 |
T3328 |
compound |
ADAM11,protein |
R906 |
T3328 |
T3326 |
pobj |
protein,of |
R907 |
T3329 |
T3322 |
aux |
would,have |
R908 |
T3330 |
T3331 |
det |
no,function |
R909 |
T3331 |
T3322 |
dobj |
function,have |
R910 |
T3332 |
T3333 |
punct |
[,26 |
R911 |
T3333 |
T3322 |
parataxis |
26,have |
R912 |
T3334 |
T3333 |
punct |
],26 |
R913 |
T3335 |
T3300 |
punct |
.,concluded |
R914 |
T3337 |
T3338 |
nsubjpass |
Absence,confirmed |
R915 |
T3339 |
T3337 |
prep |
of,Absence |
R916 |
T3340 |
T3341 |
amod |
mature,protein |
R917 |
T3341 |
T3339 |
pobj |
protein,of |
R918 |
T3342 |
T3341 |
compound |
ADAM11,protein |
R919 |
T3343 |
T3337 |
prep |
in,Absence |
R920 |
T3344 |
T3345 |
amod |
homozygous,mutants |
R921 |
T3345 |
T3343 |
pobj |
mutants,in |
R922 |
T3346 |
T3338 |
auxpass |
was,confirmed |
R923 |
T3347 |
T3338 |
prep |
by,confirmed |
R924 |
T3348 |
T3349 |
compound |
Western,blot |
R925 |
T3349 |
T3350 |
compound |
blot,analysis |
R926 |
T3350 |
T3347 |
pobj |
analysis,by |
R927 |
T3351 |
T3350 |
acl |
using,analysis |
R928 |
T3352 |
T3353 |
det |
a,antibody |
R929 |
T3353 |
T3351 |
dobj |
antibody,using |
R930 |
T3354 |
T3353 |
amod |
specific,antibody |
R931 |
T3355 |
T3356 |
dep |
that,recognises |
R932 |
T3356 |
T3353 |
relcl |
recognises,antibody |
R933 |
T3357 |
T3358 |
det |
the,protein |
R934 |
T3358 |
T3356 |
dobj |
protein,recognises |
R935 |
T3359 |
T3358 |
compound |
ADAM11,protein |
R936 |
T3360 |
T3361 |
punct |
(,1C |
R937 |
T3361 |
T3338 |
parataxis |
1C,confirmed |
R938 |
T3362 |
T3361 |
compound |
Fig.,1C |
R939 |
T3363 |
T3361 |
punct |
),1C |
R940 |
T3364 |
T3338 |
punct |
.,confirmed |
R941 |
T3366 |
T3367 |
nsubj |
Analysis,showed |
R942 |
T3368 |
T3366 |
prep |
by,Analysis |
R943 |
T3369 |
T3370 |
compound |
Western,blot |
R944 |
T3370 |
T3368 |
pobj |
blot,by |
R945 |
T3371 |
T3372 |
mark |
that,produced |
R946 |
T3372 |
T3367 |
ccomp |
produced,showed |
R947 |
T3373 |
T3374 |
amod |
heterozygous,mutants |
R948 |
T3374 |
T3372 |
nsubj |
mutants,produced |
R949 |
T3375 |
T3376 |
advmod |
approximately,half |
R950 |
T3376 |
T3372 |
dobj |
half,produced |
R951 |
T3377 |
T3376 |
prep |
of,half |
R952 |
T3378 |
T3379 |
det |
the,levels |
R953 |
T3379 |
T3377 |
pobj |
levels,of |
R954 |
T3380 |
T3381 |
amod |
wild,type |
R955 |
T3381 |
T3379 |
compound |
type,levels |
R956 |
T3382 |
T3381 |
punct |
-,type |
R957 |
T3383 |
T3379 |
prep |
of,levels |
R958 |
T3384 |
T3385 |
compound |
ADAM11,protein |
R959 |
T3385 |
T3383 |
pobj |
protein,of |
R960 |
T3386 |
T3367 |
punct |
.,showed |
R845 |
T3265 |
T3257 |
advcl |
introduced,synthesised |
R874 |
T3295 |
T3300 |
advcl |
removed,concluded |