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PMC:1420271 / 4220-14069 JSONTXT

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craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T3792 67-72 NCBITaxon:10088 denotes mouse
T3793 73-77 PR:000006847 denotes ESG1
T3794 78-82 SO:0000704 denotes gene
T3795 87-97 SO:0000336 denotes psedogenes
T3796 148-153 NCBITaxon:10088 denotes mouse
T3797 154-158 PR:000006847 denotes ESG1
T3798 159-163 SO:0000704 denotes gene
T3799 168-179 SO:0000336 denotes pseudogenes
T3800 218-223 NCBITaxon:10088 denotes mouse
T3801 224-231 SO:0001026 denotes genomic
T3802 250-254 PR:000006847 denotes ESG1
T3803 312-323 SO:0000336 denotes pseudogenes
T3804 332-339 SO:0000188 denotes introns
T3805 416-422 SO:0000188 denotes intron
T3806 428-439 SO:0000336 denotes pseudogenes
T3807 543-555 SO:0000336 denotes pseudodgenes
T3808 573-581 SO:0000857 denotes homology
T3809 585-589 PR:000006847 denotes ESG1
T3810 726-732 SO:0000188 denotes intron
T3811 738-749 SO:0000336 denotes pseudogenes
T3812 801-817 SO:0000043 denotes retropseudogenes
T3813 861-866 SO:0000704 denotes genes
T3814 1176-1180 PR:000006847 denotes ESG1
T3815 1181-1185 SO:0000704 denotes gene
T3816 1213-1220 SO:0000188 denotes introns
T3817 1254-1259 SO:0000147 denotes exons
T3818 1310-1314 PR:000006847 denotes ESG1
T3819 1349-1353 SO:0000147 denotes exon
T3820 1403-1407 PR:000006847 denotes ESG1
T3821 1408-1412 SO:0000704 denotes gene
T3822 1521-1525 SO:0000147 denotes exon
T3823 1533-1537 PR:000006847 denotes ESG1
T3824 1538-1542 SO:0000704 denotes gene
T3825 1578-1582 PR:000006847 denotes ESG1
T3826 1688-1692 PR:000006847 denotes ESG1
T3827 1693-1697 SO:0000704 denotes gene
T3828 1715-1724 NCBITaxon:2 denotes bacterial
T3829 1715-1746 SO:0000153 denotes bacterial artificial chromosome
T3830 1748-1751 SO:0000153 denotes BAC
T3831 1775-1781 SO:0000112 denotes primer
T3832 1821-1825 SO:0000704 denotes gene
T3833 1835-1846 SO:0000336 denotes pseudogenes
T3834 1893-1896 SO:0000153 denotes BAC
T3835 2029-2033 PR:000006847 denotes ESG1
T3836 2076-2083 SO:0000188 denotes introns
T3837 2105-2111 SO:0000188 denotes intron
T3838 2188-2209 SO:0001421 denotes exon-intron junctions
T3876 4442-4453 SO:0000336 denotes pseudogenes
T3877 4524-4534 SO:0000336 denotes pseudogene
T6124 4564-4569 NCBITaxon:10088 denotes mouse
T6125 4570-4574 PR:000006847 denotes ESG1
T6126 4575-4579 SO:0000704 denotes gene
T6127 4605-4609 PR:000006847 denotes ESG1
T6128 4626-4630 SO:0000704 denotes gene
T6129 4662-4667 NCBITaxon:10088 denotes mouse
T6130 4668-4676 CL:0002322 denotes ES cells
T6131 4700-4705 SO:0000147 denotes exons
T6132 4734-4742 CHEBI:7507 denotes neomycin
T6133 4774-4779 SO:0000704 denotes genes
T6134 4795-4805 CHEBI:24753 denotes hygromycin
T6135 4816-4820 SO:0000704 denotes gene
T6136 4838-4855 SO:0001644 denotes targeting vectors
T6137 4888-4896 CL:0002322 denotes ES cells
T6138 4935-4942 CL:0002322 denotes ES cell
T6139 5001-5017 SO:0001644 denotes targeting vector
T6140 5155-5171 SO:0001644 denotes targeting vector
T6171 7812-7816 SO:0000704 denotes gene
T6172 7851-7855 PR:000006847 denotes ESG1
T6173 7859-7867 CL:0002322 denotes ES cells
T6174 7885-7895 GO:0010467 denotes expression
T6175 7899-7906 CL:0002322 denotes ES cell
T6176 7914-7919 SO:0000704 denotes genes
T6177 7929-7934 PR:000010968 denotes Nanog
T6178 7939-7943 PR:000013044 denotes Oct3
T6179 7961-7965 PR:000006847 denotes ESG1
T6180 7969-7977 CL:0002322 denotes ES cells
T6181 7999-8003 PR:000013044 denotes Oct3
T6182 8006-8016 GO:0010467 denotes expression
T6183 8076-8080 SO:0000704 denotes gene
T6184 8076-8091 GO:0010467 denotes gene expression
T6185 8130-8138 CL:0002322 denotes ES cells
T6186 8143-8147 PR:000006847 denotes ESG1
T6187 8151-8159 CL:0002322 denotes ES cells
T6188 8169-8174 SO:0000704 denotes genes
T6189 8222-8226 PR:000006847 denotes ESG1
T6190 8247-8251 PR:000009450 denotes Krt1
T6191 8255-8258 PR:000014397 denotes Pem
T6192 8260-8264 PR:000003172 denotes Ctgf
T6193 8266-8271 PR:000013428 denotes Ptgs2
T6194 8282-8287 PR:000000216 denotes Inhba
T6195 8295-8300 SO:0000704 denotes genes
T6196 8310-8319 GO:0065007 denotes regulated
T6197 8346-8350 PR:000006847 denotes ESG1
T6198 8358-8362 PR:000006847 denotes ESG1
T6199 8394-8400 SO:0000417 denotes domain
T6200 8433-8438 SO:0000704 denotes genes
T6201 8955-8956 NCBITaxon:10088 denotes e
T6202 8961-8965 PR:000006847 denotes ESG1
T6203 8975-8979 NCBITaxon:10088 denotes mice
T6204 8999-9003 PR:000006847 denotes ESG1
T6205 9007-9014 CL:0002322 denotes ES cell
T6206 9031-9042 UBERON:0000358 denotes blastocysts
T6207 9053-9057 NCBITaxon:10088 denotes mice
T6208 9124-9128 PR:000006847 denotes ESG1
T6209 9132-9136 NCBITaxon:10088 denotes mice
T6210 9179-9183 PR:000006847 denotes ESG1
T6211 9195-9199 PR:000006847 denotes ESG1
T6212 9225-9229 PR:000006847 denotes ESG1
T6213 9233-9237 NCBITaxon:10088 denotes mice
T6214 9245-9252 NCBITaxon:33208 denotes animals
T6215 9356-9362 UBERON:0000473 denotes testis
T6216 9367-9372 UBERON:0000992 denotes ovary
T6217 9452-9456 PR:000006847 denotes ESG1
T6218 9481-9486 NCBITaxon:10088 denotes mouse
T6219 9503-9512 CL:0000586 denotes germ cell
T6220 9503-9522 GO:0007281 denotes germ cell formation
T6221 9542-9550 CL:0002322 denotes ES cells
T6222 9556-9567 UBERON:0000358 denotes blastocysts
T6223 9596-9600 PR:000006847 denotes ESG1
T6224 9614-9618 PR:000006847 denotes ESG1
T6225 9644-9651 CL:0002322 denotes ES cell
T6226 9687-9691 PR:000006847 denotes ESG1
T6227 9702-9706 PR:000006847 denotes ESG1
T6228 9712-9720 CL:0002322 denotes ES cells
T6229 9753-9766 GO:0008283 denotes proliferation
T6230 9817-9821 PR:000006847 denotes ESG1
T6231 9840-9848 CL:0002322 denotes ES cells
T12038 883-887 PR:000006847 denotes ESG1
T12039 887-898 SO:0000336 denotes pseudogenes
T12040 931-936 NCBITaxon:10088 denotes mouse
T12041 937-944 SO:0001026 denotes genomic
T12042 1009-1015 SO:0000188 denotes Intron
T12043 1021-1024 SO:0000730 denotes gap
T12236 2560-2563 SO:0000153 denotes BAC
T12237 2586-2590 PR:000006847 denotes ESG1
T12238 2591-2595 SO:0000704 denotes gene
T12239 2614-2624 SO:0000336 denotes pseudogene
T12240 2626-2628 SO:0000336 denotes PS
T12241 2654-2658 SO:0000704 denotes gene
T12242 2663-2665 SO:0000336 denotes PS
T12243 2713-2717 SO:0000704 denotes gene
T12244 2722-2724 SO:0000336 denotes PS
T12245 2749-2759 SO:0000857 denotes homologous
T12246 2771-2781 SO:0000028 denotes base pairs
T12247 2804-2808 SO:0000147 denotes exon
T12248 2816-2818 SO:0000028 denotes bp
T12249 2843-2847 SO:0000147 denotes exon
T12508 2960-2968 SO:0000167 denotes Promoter
T12509 2969-2977 SO:0000165 denotes enhancer
T12510 2994-2998 PR:000006847 denotes ESG1
T12511 2999-3003 SO:0000704 denotes gene
T12512 3008-3018 SO:0000336 denotes pseudogene
T12513 3041-3043 SO:0000028 denotes bp
T12514 3062-3081 SO:0001416 denotes 5' flanking regions
T12515 3089-3093 SO:0000704 denotes gene
T12516 3098-3100 SO:0000336 denotes PS
T12517 3143-3151 SO:0000155 denotes plasmids
T12518 3180-3185 SO:0000704 denotes genes
T12519 3208-3216 CL:0002322 denotes ES cells
T12520 3233-3246 CHEBI:26536 denotes retinoic acid
T12521 3255-3263 CL:0002322 denotes ES cells
T13011 5210-5215 NCBITaxon:10088 denotes mouse
T13012 5216-5220 PR:000006847 denotes ESG1
T13013 5221-5225 SO:0000704 denotes gene
T13014 5299-5316 SO:0000409 denotes Recognition sites
T13015 5402-5406 SO:0000704 denotes gene
T13016 5427-5432 CHEBI:27026 denotes toxin
T13017 5475-5492 SO:0001644 denotes targeting vectors
T13018 5604-5612 CL:0002322 denotes ES cells
T13019 5627-5635 CL:0002322 denotes ES cells
T13020 5649-5657 CL:0002322 denotes ES cells
T13021 5664-5668 PR:000006847 denotes ESG1
T13022 5674-5682 CL:0002322 denotes ES cells
T13023 5788-5796 CL:0002322 denotes ES cells
T13024 5803-5807 PR:000006847 denotes ESG1
T13025 5813-5821 CL:0002322 denotes ES cells
T13026 5859-5867 CL:0002322 denotes ES cells
T13027 5981-5997 CHEBI:4883 denotes ethidium bromide
T13028 6010-6019 GO:0005840 denotes ribosomal
T13541 7113-7117 PR:000006847 denotes ESG1
T13542 7123-7131 CL:0002322 denotes ES cells
T13543 7154-7158 PR:000006847 denotes ESG1
T13544 7164-7171 CL:0002322 denotes ES cell
T13545 7181-7186 GO:0040007 denotes grown
T13546 7243-7247 PR:000006847 denotes ESG1
T13547 7282-7290 CL:0002322 denotes ES cells
T13548 7566-7570 PR:000006847 denotes ESG1
T13549 7576-7584 CL:0002322 denotes ES cells
T13550 7586-7597 CHEBI:51686 denotes hematoxylin
T13911 8508-8512 SO:0000704 denotes Gene
T13912 8508-8523 GO:0010467 denotes Gene expression
T13913 8536-8540 PR:000006847 denotes ESG1
T13914 8546-8554 CL:0002322 denotes ES cells
T13915 8609-8617 CL:0002322 denotes ES cells
T13916 8622-8626 PR:000006847 denotes ESG1
T13917 8632-8640 CL:0002322 denotes ES cells
T13918 8659-8662 CHEBI:37987 denotes Cy3
T13919 8667-8670 CHEBI:37989 denotes Cy5
T13920 8699-8709 GO:0097617 denotes hybridized
T13921 8721-8726 NCBITaxon:10088 denotes Mouse
T13922 8848-8852 PR:000006847 denotes ESG1
T13923 8860-8864 PR:000006847 denotes ESG1
T13924 8892-8902 GO:0010467 denotes expression
T13925 8906-8910 PR:000006847 denotes ESG1
T13926 8912-8916 PR:000013044 denotes Oct3
T13927 8923-8927 PR:000005257 denotes CDK4
T3839 2220-2238 SO:0001416 denotes 5' flanking region
T3840 2277-2285 SO:0000167 denotes promoter
T3841 2286-2294 SO:0000165 denotes enhancer
T3842 2354-2362 CL:0002322 denotes ES cells
T3843 2375-2388 CL:0002371 denotes somatic cells
T3844 2485-2498 CHEBI:26536 denotes retinoic acid
T3845 2539-2543 PR:000006847 denotes ESG1
T3846 2544-2548 SO:0000704 denotes gene
T3847 3428-3431 SO:0000153 denotes BAC
T3848 3457-3461 PR:000006847 denotes ESG1
T3849 3527-3529 SO:0000028 denotes bp
T3850 3530-3549 SO:0000605 denotes intergenic sequence
T3851 3593-3597 PR:000006847 denotes ESG1
T3852 3655-3660 SO:0000147 denotes exons
T3853 3665-3672 SO:0000188 denotes introns
T3854 3680-3684 PR:000006847 denotes ESG1
T3855 3685-3689 SO:0000704 denotes gene
T3856 3773-3778 SO:0000147 denotes exons
T3857 3811-3815 SO:0000147 denotes exon
T3858 3844-3854 SO:0000028 denotes base pairs
T3859 3862-3881 SO:0001417 denotes 3' flanking regions
T3860 3909-3913 PR:000006847 denotes ESG1
T3861 3914-3918 SO:0000704 denotes gene
T3862 3927-3937 SO:0000336 denotes pseudogene
T3863 3949-3959 SO:0000028 denotes base pairs
T3864 3967-3985 SO:0001416 denotes 5' flanking region
T3865 4007-4025 SO:0001416 denotes 5' flanking region
T3866 4031-4033 SO:0000028 denotes bp
T3867 4055-4063 SO:0000167 denotes promoter
T3868 4064-4072 SO:0000165 denotes enhancer
T3869 4181-4191 GO:0006412 denotes translated
T3870 4265-4275 SO:0000336 denotes pseudogene
T3871 4327-4338 SO:0000336 denotes pseudogenes
T3872 4352-4356 SO:0000147 denotes exon
T3873 4357-4363 SO:0000188 denotes intron
T3874 4384-4388 PR:000006847 denotes ESG1
T3875 4389-4393 SO:0000704 denotes gene
T6141 6076-6084 CL:0002322 denotes ES cells
T6142 6110-6116 SO:0000440 denotes vector
T6143 6140-6148 CL:0002322 denotes ES cells
T6144 6157-6161 CHEBI:42768 denotes G418
T6145 6212-6216 PR:000006847 denotes ESG1
T6146 6292-6296 PR:000006847 denotes ESG1
T6147 6349-6355 SO:0000440 denotes vector
T6148 6378-6384 SO:0000440 denotes vector
T6149 6467-6473 SO:0000440 denotes vector
T6150 6514-6518 PR:000006847 denotes ESG1
T6151 6522-6530 CL:0002322 denotes ES cells
T6152 6589-6601 GO:0008283 denotes proliferated
T6153 6695-6699 PR:000006847 denotes ESG1
T6154 6754-6780 PR:000009799 denotes leukemia inhibitory factor
T6155 6796-6809 CHEBI:26536 denotes retinoic acid
T6156 6861-6867 UBERON:0000180 denotes flanks
T6157 6876-6880 NCBITaxon:10088 denotes mice
T6158 6956-6967 UBERON:0000923 denotes germ layers
T6159 7009-7013 PR:000006847 denotes ESG1
T6160 7037-7049 GO:0017145 denotes self-renewal
T6161 7081-7089 CL:0002322 denotes ES cells
T6162 7620-7621 CL:0002322 denotes e
T6163 7621-7622 CHEBI:51686 denotes x
T6164 7633-7637 SO:0000704 denotes gene
T6165 7633-7648 GO:0010467 denotes gene expression
T6166 7661-7665 PR:000006847 denotes ESG1
T6167 7669-7677 CL:0002322 denotes ES cells
T6168 7743-7748 SO:0000704 denotes genes
T6169 7775-7783 CL:0002322 denotes ES cells
T6170 7785-7789 PR:000006847 denotes ESG1

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T3878 24-35 GO_SO_EXT:chromosome denotes Chromosomal
T3879 36-48 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T3880 67-72 NCBITaxon:10088 denotes mouse
T3881 73-77 PR_EXT:000006847 denotes ESG1
T3894 260-268 SO_EXT:biological_sequence denotes sequence
T3895 312-323 SO_EXT:0000336 denotes pseudogenes
T3896 332-339 SO_EXT:0000188 denotes introns
T3897 343-354 GO_SO_EXT:chromosome denotes chromosomes
T3898 416-422 SO_EXT:0000188 denotes intron
T3899 428-439 SO_EXT:0000336 denotes pseudogenes
T3900 459-462 CHEBI_SO_EXT:DNA denotes DNA
T3901 487-498 GO_SO_EXT:chromosome denotes chromosomal
T3902 499-511 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T3903 543-555 SO_EXT:0000336 denotes pseudodgenes
T3904 573-581 SO:0000857 denotes homology
T3905 585-589 PR_EXT:000006847 denotes ESG1
T3906 590-594 SO_EXT:cDNA denotes cDNA
T3907 630-638 CHEBI_PR_EXT:protein denotes proteins
T3908 660-669 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T3909 726-732 SO_EXT:0000188 denotes intron
T3910 738-749 SO_EXT:0000336 denotes pseudogenes
T3911 801-817 SO_EXT:0000043 denotes retropseudogenes
T3912 847-851 CL_GO_EXT:cell denotes cell
T3913 861-866 SO_EXT:0000704 denotes genes
T3914 1118-1128 GO_SO_EXT:chromosome denotes chromosome
T3915 1148-1151 CHEBI_SO_EXT:DNA denotes DNA
T3916 1176-1180 PR_EXT:000006847 denotes ESG1
T3917 1181-1185 SO_EXT:0000704 denotes gene
T3918 1213-1220 SO_EXT:0000188 denotes introns
T3919 1254-1259 SO_EXT:0000147 denotes exons
T3920 1293-1302 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T3921 1310-1314 PR_EXT:000006847 denotes ESG1
T3922 1315-1319 SO_EXT:cDNA denotes cDNA
T3923 1320-1328 SO_EXT:biological_sequence denotes sequence
T3924 1349-1353 SO_EXT:0000147 denotes exon
T3925 1403-1407 PR_EXT:000006847 denotes ESG1
T3926 1408-1412 SO_EXT:0000704 denotes gene
T3927 1422-1432 GO_SO_EXT:chromosome denotes chromosome
T3928 1458-1461 CHEBI_SO_EXT:DNA denotes DNA
T3929 1521-1525 SO_EXT:0000147 denotes exon
T3930 1533-1537 PR_EXT:000006847 denotes ESG1
T3931 1538-1542 SO_EXT:0000704 denotes gene
T3932 1578-1582 PR_EXT:000006847 denotes ESG1
T3933 1588-1597 SO_EXT:biological_sequence denotes sequences
T3934 1601-1611 GO_SO_EXT:chromosome denotes chromosome
T3935 1659-1662 CHEBI_SO_EXT:DNA denotes DNA
T3936 1688-1692 PR_EXT:000006847 denotes ESG1
T3937 1693-1697 SO_EXT:0000704 denotes gene
T3938 1715-1724 NCBITaxon:2 denotes bacterial
T3939 1715-1746 SO_EXT:0000153 denotes bacterial artificial chromosome
T3940 1736-1746 GO_SO_EXT:chromosome denotes chromosome
T3941 1748-1751 SO_EXT:0000153 denotes BAC
T3942 1753-1756 CHEBI_SO_EXT:DNA denotes DNA
T3943 1775-1781 SO_EXT:0000112 denotes primer
T3944 1821-1825 SO_EXT:0000704 denotes gene
T3945 1835-1846 SO_EXT:0000336 denotes pseudogenes
T3946 1893-1896 SO_EXT:0000153 denotes BAC
T3947 1897-1903 SO_EXT:sequence_cloned_entity denotes clones
T3948 1967-1973 SO_EXT:sequence_cloned_entity denotes clones
T3949 1986-1994 SO_EXT:biological_sequence denotes sequence
T3950 2029-2033 PR_EXT:000006847 denotes ESG1
T3951 2034-2038 SO_EXT:cDNA denotes cDNA
T3952 2076-2083 SO_EXT:0000188 denotes introns
T3953 2105-2111 SO_EXT:0000188 denotes intron
T3954 2112-2121 SO_EXT:biological_sequence denotes sequences
T4017 4389-4393 SO_EXT:0000704 denotes gene
T4018 4442-4453 SO_EXT:0000336 denotes pseudogenes
T4019 4524-4534 SO_EXT:0000336 denotes pseudogene
T6232 4564-4569 NCBITaxon:10088 denotes mouse
T6233 4570-4574 PR_EXT:000006847 denotes ESG1
T6234 4575-4579 SO_EXT:0000704 denotes gene
T6235 4605-4609 PR_EXT:000006847 denotes ESG1
T6236 4614-4621 SO_EXT:sequence_deletion_process denotes deleted
T6237 4626-4630 SO_EXT:0000704 denotes gene
T6238 4645-4658 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T6239 4662-4667 NCBITaxon:10088 denotes mouse
T6240 4668-4676 CL:0002322 denotes ES cells
T6241 4671-4676 CL_GO_EXT:cell denotes cells
T6242 4681-4689 SO_EXT:sequence_substitution_process denotes replaced
T6243 4700-4705 SO_EXT:0000147 denotes exons
T6244 4720-4729 _FRAGMENT denotes fusion of
T6245 4774-4779 SO_EXT:fusion_gene_or_gene_fusion denotes genes
T6246 4734-4742 CHEBI:7507 denotes neomycin
T6247 4758-4773 GO_EXT:0004565 denotes β-galactosidase
T6248 4781-4786 GO_EXT:beta_geo denotes β-geo
T6249 4795-4805 CHEBI:24753 denotes hygromycin
T6250 4816-4820 SO_EXT:0000704 denotes gene
T6251 4838-4855 SO_EXT:0001644 denotes targeting vectors
T6252 4888-4896 CL:0002322 denotes ES cells
T6253 4891-4896 CL_GO_EXT:cell denotes cells
T6254 4935-4942 CL:0002322 denotes ES cell
T6255 4938-4942 CL_GO_EXT:cell denotes cell
T6256 4943-4949 SO_EXT:sequence_cloned_entity denotes clones
T6257 4974-4987 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T6258 4995-5000 GO_EXT:beta_geo denotes β-geo
T6259 5001-5017 SO_EXT:0001644 denotes targeting vector
T6260 5099-5104 SO_EXT:sequence_cloned_entity denotes clone
T6261 5129-5142 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T6262 5155-5171 SO_EXT:0001644 denotes targeting vector
T6301 6888-6893 CL_GO_EXT:cell denotes cells
T6302 6956-6967 UBERON:0000923 denotes germ layers
T6303 7009-7013 PR_EXT:000006847 denotes ESG1
T6304 7037-7049 GO:0017145 denotes self-renewal
T6305 7081-7089 CL:0002322 denotes ES cells
T6306 7084-7089 CL_GO_EXT:cell denotes cells
T6307 7617-7618 SO_EXT:wild_type_entity_or_quality denotes W
T6327 7857-7858 SO_EXT:sequence_nullness_or_absence denotes -
T6328 7859-7867 CL:0002322 denotes ES cells
T6329 7862-7867 CL_GO_EXT:cell denotes cells
T6330 7885-7895 GO:0010467 denotes expression
T6331 7899-7906 CL:0002322 denotes ES cell
T6332 7902-7906 CL_GO_EXT:cell denotes cell
T6333 7907-7913 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T6334 7914-7919 SO_EXT:0000704 denotes genes
T6335 7929-7934 PR_EXT:000010968 denotes Nanog
T6336 7939-7943 PR_EXT:000013044 denotes Oct3
T6337 7961-7965 PR_EXT:000006847 denotes ESG1
T6338 7965-7966 SO_EXT:sequence_nullness_or_absence denotes -
T6339 7967-7968 SO_EXT:sequence_nullness_or_absence denotes -
T6340 7969-7977 CL:0002322 denotes ES cells
T6341 7972-7977 CL_GO_EXT:cell denotes cells
T6342 7999-8003 PR_EXT:000013044 denotes Oct3
T6343 8006-8016 GO:0010467 denotes expression
T6344 8020-8027 CHEBI_PR_EXT:protein denotes protein
T6345 8076-8080 SO_EXT:0000704 denotes gene
T6346 8076-8091 GO:0010467 denotes gene expression
T6347 8130-8138 CL:0002322 denotes ES cells
T6348 8133-8138 CL_GO_EXT:cell denotes cells
T6349 8143-8147 PR_EXT:000006847 denotes ESG1
T6350 8147-8148 SO_EXT:sequence_nullness_or_absence denotes -
T6351 8149-8150 SO_EXT:sequence_nullness_or_absence denotes -
T6352 8151-8159 CL:0002322 denotes ES cells
T6353 8154-8159 CL_GO_EXT:cell denotes cells
T6354 8169-8174 SO_EXT:0000704 denotes genes
T6355 8222-8226 PR_EXT:000006847 denotes ESG1
T6356 8226-8227 SO_EXT:sequence_nullness_or_absence denotes -
T6357 8228-8229 SO_EXT:sequence_nullness_or_absence denotes -
T6358 8230-8235 CL_GO_EXT:cell denotes cells
T6359 8247-8251 PR_EXT:000009450 denotes Krt1
T6360 8255-8258 PR_EXT:000014397 denotes Pem
T6361 8260-8264 PR_EXT:000003172 denotes Ctgf
T6362 8266-8271 PR_EXT:000013428 denotes Ptgs2
T6363 8273-8277 CHEBI_PR_EXT:insulin_like_growth_factor_2 denotes Igf2
T6364 8282-8287 PR_EXT:000000216 denotes Inhba
T6365 8295-8300 SO_EXT:0000704 denotes genes
T6366 8310-8319 GO:0065007 denotes regulated
T6367 8346-8350 PR_EXT:000006847 denotes ESG1
T6368 8358-8362 PR_EXT:000006847 denotes ESG1
T6369 8382-8385 CHEBI_SO_EXT:RNA denotes RNA
T6370 8386-8393 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T6371 8394-8400 SO_EXT:0000417 denotes domain
T6372 8419-8423 CHEBI_SO_EXT:mRNA denotes mRNA
T6373 8433-8438 SO_EXT:0000704 denotes genes
T6401 9231-9232 SO_EXT:sequence_nullness_or_absence denotes -
T6403 9245-9252 NCBITaxon:33208 denotes animals
T6404 9356-9362 UBERON:0000473 denotes testis
T6405 9367-9372 UBERON:0000992 denotes ovary
T6406 9452-9456 PR_EXT:000006847 denotes ESG1
T6407 9481-9486 NCBITaxon:10088 denotes mouse
T6408 9503-9512 CL:0000586 denotes germ cell
T6409 9503-9522 GO:0007281 denotes germ cell formation
T6410 9508-9512 CL_GO_EXT:cell denotes cell
T6411 9542-9550 CL:0002322 denotes ES cells
T6412 9545-9550 CL_GO_EXT:cell denotes cells
T6413 9556-9567 UBERON:0000358 denotes blastocysts
T6414 9596-9600 PR_EXT:000006847 denotes ESG1
T6415 9600-9601 SO_EXT:normal_or_wild_type_or_present denotes +
T6416 9602-9603 SO_EXT:sequence_nullness_or_absence denotes -
T6417 9604-9609 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes males
T6418 9614-9618 PR_EXT:000006847 denotes ESG1
T6419 9618-9619 SO_EXT:sequence_nullness_or_absence denotes -
T6420 9620-9621 SO_EXT:sequence_nullness_or_absence denotes -
T6421 9622-9629 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T6422 9644-9651 CL:0002322 denotes ES cell
T6423 9647-9651 CL_GO_EXT:cell denotes cell
T6424 9675-9681 SO_EXT:sequence_cloned_entity denotes clones
T6425 9687-9691 PR_EXT:000006847 denotes ESG1
T6426 9691-9692 SO_EXT:sequence_nullness_or_absence denotes -
T6427 9693-9694 SO_EXT:sequence_nullness_or_absence denotes -
T6428 9702-9706 PR_EXT:000006847 denotes ESG1
T6429 9707-9711 SO_EXT:sequence_nullness denotes null
T6430 9712-9720 CL:0002322 denotes ES cells
T6431 9715-9720 CL_GO_EXT:cell denotes cells
T6432 9753-9766 GO:0008283 denotes proliferation
T6433 9772-9787 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T6434 9817-9821 PR_EXT:000006847 denotes ESG1
T6435 9840-9848 CL:0002322 denotes ES cells
T6436 9843-9848 CL_GO_EXT:cell denotes cells
T12044 883-887 PR_EXT:000006847 denotes ESG1
T12045 887-898 SO_EXT:0000336 denotes pseudogenes
T12046 931-936 NCBITaxon:10088 denotes mouse
T12047 937-944 SO_EXT:0001026 denotes genomic
T12048 956-968 SO_EXT:sequence_substitution_entity_or_process denotes Substitution
T12049 969-978 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T12050 1009-1015 SO_EXT:0000188 denotes Intron
T12051 1021-1024 SO_EXT:0000730 denotes gap
T12052 1025-1034 SO_EXT:biological_sequence denotes sequences
T12053 1065-1076 GO_SO_EXT:chromosome denotes Chromosomal
T12054 1077-1090 GO_PATO_EXT:biological_localization_process_or_quality denotes localizations
T12250 2560-2563 SO_EXT:0000153 denotes BAC
T12251 2564-2570 SO_EXT:sequence_cloned_entity denotes clones
T12252 2586-2590 PR_EXT:000006847 denotes ESG1
T12253 2591-2595 SO_EXT:0000704 denotes gene
T12254 2602-2613 SO_EXT:sequence_duplication_entity_or_process denotes duplication
T12255 2614-2624 SO_EXT:0000336 denotes pseudogene
T12256 2626-2628 SO_EXT:0000336 denotes PS
T12257 2634-2646 GO_PATO_EXT:biological_localization_process_or_quality denotes Localization
T12258 2654-2658 SO_EXT:0000704 denotes gene
T12259 2663-2665 SO_EXT:0000336 denotes PS
T12260 2669-2679 GO_SO_EXT:chromosome denotes chromosome
T12261 2686-2694 SO_EXT:biological_sequence denotes Sequence
T12262 2713-2717 SO_EXT:0000704 denotes gene
T12263 2722-2724 SO_EXT:0000336 denotes PS
T12264 2749-2759 SO:0000857 denotes homologous
T12265 2771-2775 CHEBI_SO_EXT:base denotes base
T12266 2771-2781 SO_EXT:0000028 denotes base pairs
T12267 2782-2790 SO_EXT:sequence_upstreamness denotes upstream
T12268 2804-2808 SO_EXT:0000147 denotes exon
T12269 2816-2818 SO_EXT:0000028 denotes bp
T12270 2819-2829 SO_EXT:sequence_downstreamness denotes downstream
T12271 2843-2847 SO_EXT:0000147 denotes exon
T12272 2849-2861 SO_EXT:sequence_substitution_entity_or_process denotes Substitution
T12273 2862-2871 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T12274 2906-2915 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T12522 2960-2968 SO_EXT:0000167 denotes Promoter
T12523 2969-2977 SO_EXT:0000165 denotes enhancer
T12524 2994-2998 PR_EXT:000006847 denotes ESG1
T12525 2999-3003 SO_EXT:0000704 denotes gene
T12526 3008-3018 SO_EXT:0000336 denotes pseudogene
T12527 3020-3023 CHEBI_SO_EXT:DNA denotes DNA
T12528 3041-3043 SO_EXT:0000028 denotes bp
T12529 3062-3081 SO_EXT:0001416 denotes 5' flanking regions
T12530 3089-3093 SO_EXT:0000704 denotes gene
T12531 3098-3100 SO_EXT:0000336 denotes PS
T12532 3106-3117 GO_EXT:biological_movement_or_translocation_process denotes transferred
T12533 3123-3133 GO_EXT:0045289 denotes luciferase
T12534 3143-3151 SO_EXT:0000155 denotes plasmids
T12535 3180-3185 SO_EXT:0000704 denotes genes
T12536 3193-3207 GO_PATO_RO_EXT:developmental_differentiation_process_or_quality denotes differentiated
T12537 3208-3216 CL:0002322 denotes ES cells
T12538 3211-3216 CL_GO_EXT:cell denotes cells
T12539 3233-3246 CHEBI:26536 denotes retinoic acid
T12540 3255-3263 CL:0002322 denotes ES cells
T12541 3258-3263 CL_GO_EXT:cell denotes cells
T12542 3291-3296 CL_GO_EXT:cell denotes cells
T13029 5210-5215 NCBITaxon:10088 denotes mouse
T13030 5216-5220 PR_EXT:000006847 denotes ESG1
T13031 5221-5225 SO_EXT:0000704 denotes gene
T13032 5299-5316 SO_EXT:0000409 denotes Recognition sites
T13033 5402-5406 SO_EXT:0000704 denotes gene
T13034 5407-5415 SO_EXT:sequence_coding_function denotes encoding
T13035 5427-5432 CHEBI_EXT:27026 denotes toxin
T13036 5445-5453 SO_EXT:sequence_insertion_process denotes inserted
T13037 5475-5492 SO_EXT:0001644 denotes targeting vectors
T13038 5575-5588 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T13039 5590-5592 SO_EXT:wild_type_entity_or_quality denotes WT
T13040 5594-5603 SO_EXT:wild_type_entity_or_quality denotes wild-type
T13041 5604-5612 CL:0002322 denotes ES cells
T13042 5607-5612 CL_GO_EXT:cell denotes cells
T13043 5617-5622 GO_EXT:beta_geo denotes β-geo
T13044 5623-5624 SO_EXT:normal_or_wild_type_or_present denotes +
T13045 5625-5626 SO_EXT:sequence_nullness_or_absence denotes -
T13046 5627-5635 CL:0002322 denotes ES cells
T13047 5630-5635 CL_GO_EXT:cell denotes cells
T13048 5645-5646 SO_EXT:normal_or_wild_type_or_present denotes +
T13049 5647-5648 SO_EXT:sequence_nullness_or_absence denotes -
T13050 5649-5657 CL:0002322 denotes ES cells
T13051 5652-5657 CL_GO_EXT:cell denotes cells
T13052 5659-5660 SO_EXT:sequence_nullness_or_absence denotes -
T13053 5661-5662 SO_EXT:sequence_nullness_or_absence denotes -
T13054 5664-5668 PR_EXT:000006847 denotes ESG1
T13055 5669-5673 SO_EXT:sequence_nullness denotes null
T13056 5674-5682 CL:0002322 denotes ES cells
T13057 5677-5682 CL_GO_EXT:cell denotes cells
T13058 5701-5706 SO_EXT:sequence_cloned_entity denotes clone
T13059 5778-5787 SO_EXT:wild_type_entity_or_quality denotes wild-type
T13060 5788-5796 CL:0002322 denotes ES cells
T13061 5791-5796 CL_GO_EXT:cell denotes cells
T13062 5798-5800 SO_EXT:wild_type_entity_or_quality denotes WT
T13063 5803-5807 PR_EXT:000006847 denotes ESG1
T13064 5808-5812 SO_EXT:sequence_nullness denotes null
T13065 5813-5821 CL:0002322 denotes ES cells
T13066 5816-5821 CL_GO_EXT:cell denotes cells
T13067 5823-5824 SO_EXT:sequence_nullness_or_absence denotes -
T13068 5825-5826 SO_EXT:sequence_nullness_or_absence denotes -
T13069 5834-5840 SO_EXT:sequence_cloned_entity denotes clones
T13070 5859-5867 CL:0002322 denotes ES cells
T13071 5862-5867 CL_GO_EXT:cell denotes cells
T13072 5869-5870 SO_EXT:normal_or_wild_type_or_present denotes +
T13073 5871-5872 SO_EXT:sequence_nullness_or_absence denotes -
T13074 5976-5979 CHEBI_SO_EXT:RNA denotes RNA
T13075 5981-5997 CHEBI:4883 denotes ethidium bromide
T13076 6010-6019 GO:0005840 denotes ribosomal
T13077 6010-6023 CHEBI_SO_EXT:rRNA denotes ribosomal RNA
T13551 7113-7117 PR_EXT:000006847 denotes ESG1
T13552 7118-7122 SO_EXT:sequence_nullness denotes null
T13553 7123-7131 CL:0002322 denotes ES cells
T13554 7126-7131 CL_GO_EXT:cell denotes cells
T13555 7154-7158 PR_EXT:000006847 denotes ESG1
T13556 7159-7163 SO_EXT:sequence_nullness denotes null
T13557 7164-7171 CL:0002322 denotes ES cell
T13558 7167-7171 CL_GO_EXT:cell denotes cell
T13559 7181-7186 GO:0040007 denotes grown
T13560 7201-7206 CL_GO_EXT:cell denotes cells
T13561 7211-7217 GO_EXT:biological_growth_entity_or_process denotes Growth
T13562 7227-7236 SO_EXT:wild_type_entity_or_quality denotes wild-type
T13563 7238-7240 SO_EXT:wild_type_entity_or_quality denotes WT
T13564 7243-7247 PR_EXT:000006847 denotes ESG1
T13565 7248-7252 SO_EXT:sequence_nullness denotes null
T13566 7254-7255 SO_EXT:sequence_nullness_or_absence denotes -
T13567 7256-7257 SO_EXT:sequence_nullness_or_absence denotes -
T13568 7277-7278 SO_EXT:normal_or_wild_type_or_present denotes +
T13569 7279-7280 SO_EXT:sequence_nullness_or_absence denotes -
T13570 7282-7290 CL:0002322 denotes ES cells
T13571 7285-7290 CL_GO_EXT:cell denotes cells
T13572 7297-7302 SO_EXT:sequence_cloned_entity denotes clone
T13573 7312-7317 CL_GO_EXT:cell denotes cells
T13574 7354-7358 CL_GO_EXT:cell denotes Cell
T13575 7435-7436 SO_EXT:normal_or_wild_type_or_present denotes +
T13576 7437-7438 SO_EXT:sequence_nullness_or_absence denotes -
T13577 7443-7444 SO_EXT:sequence_nullness_or_absence denotes -
T13578 7445-7446 SO_EXT:sequence_nullness_or_absence denotes -
T13579 7447-7452 CL_GO_EXT:cell denotes cells
T13580 7520-7526 SO_EXT:sequence_cloned_entity denotes clones
T13581 7566-7570 PR_EXT:000006847 denotes ESG1
T13582 7571-7575 SO_EXT:sequence_nullness denotes null
T13583 7576-7584 CL:0002322 denotes ES cells
T13584 7579-7584 CL_GO_EXT:cell denotes cells
T13585 7586-7597 CHEBI:51686 denotes hematoxylin
T13586 7600-7605 CHEBI_EXT:eosin denotes eosin
T13928 8508-8512 SO_EXT:0000704 denotes Gene
T13929 8508-8523 GO:0010467 denotes Gene expression
T13930 8536-8540 PR_EXT:000006847 denotes ESG1
T13931 8541-8545 SO_EXT:sequence_nullness denotes null
T13932 8546-8554 CL:0002322 denotes ES cells
T13933 8549-8554 CL_GO_EXT:cell denotes cells
T13934 8559-8562 CHEBI_SO_EXT:DNA denotes DNA
T13935 8590-8593 CHEBI_SO_EXT:RNA denotes RNA
T13936 8599-8608 SO_EXT:wild_type_entity_or_quality denotes wild-type
T13937 8609-8617 CL:0002322 denotes ES cells
T13938 8612-8617 CL_GO_EXT:cell denotes cells
T13939 8622-8626 PR_EXT:000006847 denotes ESG1
T13940 8627-8631 SO_EXT:sequence_nullness denotes null
T13941 8632-8640 CL:0002322 denotes ES cells
T13942 8635-8640 CL_GO_EXT:cell denotes cells
T13943 8646-8653 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T13944 8659-8662 CHEBI:37987 denotes Cy3
T13945 8667-8670 CHEBI:37989 denotes Cy5
T13946 8699-8709 GO:0097617 denotes hybridized
T13947 8721-8726 NCBITaxon:10088 denotes Mouse
T13948 8786-8792 SO_EXT:sequence_cloned_entity denotes clones
T13949 8830-8834 CL_GO_EXT:cell denotes Cell
T13950 8848-8852 PR_EXT:000006847 denotes ESG1
T13951 8852-8853 SO_EXT:normal_or_wild_type_or_present denotes +
T13952 8854-8855 SO_EXT:sequence_nullness_or_absence denotes -
T13953 8860-8864 PR_EXT:000006847 denotes ESG1
T13954 8864-8865 SO_EXT:sequence_nullness_or_absence denotes -
T13955 8866-8867 SO_EXT:sequence_nullness_or_absence denotes -
T13956 8868-8873 CL_GO_EXT:cell denotes cells
T13957 8892-8902 GO:0010467 denotes expression
T13958 8906-8910 PR_EXT:000006847 denotes ESG1
T13959 8912-8916 PR_EXT:000013044 denotes Oct3
T13960 8923-8927 PR_EXT:000005257 denotes CDK4
T3969 2539-2543 PR_EXT:000006847 denotes ESG1
T3970 2544-2548 SO_EXT:0000704 denotes gene
T3971 3428-3431 SO_EXT:0000153 denotes BAC
T3992 3844-3854 SO_EXT:0000028 denotes base pairs
T3993 3862-3881 SO_EXT:0001417 denotes 3' flanking regions
T3882 78-82 SO_EXT:0000704 denotes gene
T3883 87-97 SO_EXT:0000336 denotes psedogenes
T3884 115-126 GO_SO_EXT:chromosome denotes chromosomal
T3885 127-140 GO_PATO_EXT:biological_localization_process_or_quality denotes localizations
T3886 148-153 NCBITaxon:10088 denotes mouse
T3887 154-158 PR_EXT:000006847 denotes ESG1
T3888 159-163 SO_EXT:0000704 denotes gene
T3889 168-179 SO_EXT:0000336 denotes pseudogenes
T3890 218-223 NCBITaxon:10088 denotes mouse
T3891 224-231 SO_EXT:0001026 denotes genomic
T3892 250-254 PR_EXT:000006847 denotes ESG1
T3893 255-259 SO_EXT:cDNA denotes cDNA
T3955 2188-2209 SO_EXT:0001421 denotes exon-intron junctions
T3956 2220-2238 SO_EXT:0001416 denotes 5' flanking region
T3957 2247-2250 CHEBI_SO_EXT:DNA denotes DNA
T3958 2277-2285 SO_EXT:0000167 denotes promoter
T3959 2286-2294 SO_EXT:0000165 denotes enhancer
T3960 2307-2317 GO_EXT:0045289 denotes luciferase
T3961 2339-2353 GO_PATO_RO_EXT:developmental_differentiation_process_or_quality denotes differentiated
T3962 2354-2362 CL:0002322 denotes ES cells
T3963 2357-2362 CL_GO_EXT:cell denotes cells
T3964 2375-2388 CL:0002371 denotes somatic cells
T3965 2383-2388 CL_GO_EXT:cell denotes cells
T3966 2466-2481 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T3967 2485-2498 CHEBI:26536 denotes retinoic acid
T3968 2523-2531 SO_EXT:biological_sequence denotes sequence
T3972 3432-3438 SO_EXT:sequence_cloned_entity denotes clones
T3973 3457-3461 PR_EXT:000006847 denotes ESG1
T3974 3467-3475 SO_EXT:biological_sequence denotes sequence
T3975 3497-3506 SO_EXT:biological_sequence denotes sequences
T3976 3527-3529 SO_EXT:0000028 denotes bp
T3977 3530-3549 SO_EXT:0000605 denotes intergenic sequence
T3978 3593-3597 PR_EXT:000006847 denotes ESG1
T3979 3603-3611 SO_EXT:biological_sequence denotes sequence
T3980 3655-3660 SO_EXT:0000147 denotes exons
T3981 3665-3672 SO_EXT:0000188 denotes introns
T3982 3680-3684 PR_EXT:000006847 denotes ESG1
T3983 3685-3689 SO_EXT:0000704 denotes gene
T3984 3696-3704 SO_EXT:biological_sequence denotes sequence
T3985 3734-3744 CHEBI_SO_EXT:nucleotide denotes nucleotide
T3986 3745-3758 SO_EXT:sequence_substitution_entity_or_process denotes substitutions
T3987 3773-3778 SO_EXT:0000147 denotes exons
T3988 3787-3797 CHEBI_SO_EXT:nucleotide denotes nucleotide
T3989 3798-3807 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T3990 3811-3815 SO_EXT:0000147 denotes exon
T3991 3844-3848 CHEBI_SO_EXT:base denotes base
T3994 3887-3896 SO_EXT:biological_conservation_process_or_quality denotes conserved
T3995 3909-3913 PR_EXT:000006847 denotes ESG1
T3996 3914-3918 SO_EXT:0000704 denotes gene
T3997 3927-3937 SO_EXT:0000336 denotes pseudogene
T3998 3949-3953 CHEBI_SO_EXT:base denotes base
T3999 3949-3959 SO_EXT:0000028 denotes base pairs
T4000 3967-3985 SO_EXT:0001416 denotes 5' flanking region
T4001 4007-4025 SO_EXT:0001416 denotes 5' flanking region
T4002 4031-4033 SO_EXT:0000028 denotes bp
T4003 4055-4063 SO_EXT:0000167 denotes promoter
T4004 4064-4072 SO_EXT:0000165 denotes enhancer
T4005 4085-4095 GO_EXT:0045289 denotes luciferase
T4006 4154-4162 SO_EXT:biological_sequence denotes sequence
T4007 4166-4177 GO_EXT:transcription denotes transcribed
T4008 4181-4191 GO:0006412 denotes translated
T4009 4210-4217 CHEBI_PR_EXT:protein denotes protein
T4010 4224-4232 SO_EXT:biological_sequence denotes sequence
T4011 4253-4264 SO_EXT:sequence_duplication_entity_or_process denotes duplication
T4012 4265-4275 SO_EXT:0000336 denotes pseudogene
T4013 4327-4338 SO_EXT:0000336 denotes pseudogenes
T4014 4352-4356 SO_EXT:0000147 denotes exon
T4015 4357-4363 SO_EXT:0000188 denotes intron
T4016 4384-4388 PR_EXT:000006847 denotes ESG1
T6263 6076-6084 CL:0002322 denotes ES cells
T6264 6079-6084 CL_GO_EXT:cell denotes cells
T6265 6104-6109 GO_EXT:beta_geo denotes β-geo
T6266 6110-6116 SO_EXT:0000440 denotes vector
T6267 6140-6148 CL:0002322 denotes ES cells
T6268 6143-6148 CL_GO_EXT:cell denotes cells
T6269 6157-6161 CHEBI:42768 denotes G418
T6270 6212-6216 PR_EXT:000006847 denotes ESG1
T6271 6217-6225 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T6272 6292-6296 PR_EXT:000006847 denotes ESG1
T6273 6306-6311 CL_GO_EXT:cell denotes cells
T6274 6331-6337 SO_EXT:sequence_cloned_entity denotes clones
T6275 6343-6348 GO_EXT:beta_geo denotes β-geo
T6276 6349-6355 SO_EXT:0000440 denotes vector
T6277 6360-6368 SO_EXT:sequence_substitution_process denotes replaced
T6278 6378-6384 SO_EXT:0000440 denotes vector
T6279 6400-6405 CL_GO_EXT:cell denotes cells
T6280 6449-6455 SO_EXT:sequence_cloned_entity denotes clones
T6281 6461-6466 GO_EXT:beta_geo denotes β-geo
T6282 6467-6473 SO_EXT:0000440 denotes vector
T6283 6514-6518 PR_EXT:000006847 denotes ESG1
T6284 6518-6519 SO_EXT:sequence_nullness_or_absence denotes -
T6285 6520-6521 SO_EXT:sequence_nullness_or_absence denotes -
T6286 6522-6530 CL:0002322 denotes ES cells
T6287 6525-6530 CL_GO_EXT:cell denotes cells
T6288 6578-6583 CL_GO_EXT:cell denotes cells
T6289 6589-6601 GO:0008283 denotes proliferated
T6290 6665-6674 SO_EXT:wild_type_entity_or_quality denotes wild-type
T6291 6676-6681 CL_GO_EXT:cell denotes cells
T6292 6695-6699 PR_EXT:000006847 denotes ESG1
T6293 6699-6700 SO_EXT:sequence_nullness_or_absence denotes -
T6294 6701-6702 SO_EXT:sequence_nullness_or_absence denotes -
T6295 6703-6708 CL_GO_EXT:cell denotes cells
T6296 6709-6723 GO_PATO_RO_EXT:developmental_differentiation_process_or_quality denotes differentiated
T6297 6754-6780 PR_EXT:000009799 denotes leukemia inhibitory factor
T6298 6796-6809 CHEBI:26536 denotes retinoic acid
T6299 6861-6867 UBERON:0000180 denotes flanks
T6300 6876-6880 NCBITaxon:10088 denotes mice
T6308 7620-7621 CL:0002322 denotes e
T6309 7620-7621 CL_GO_EXT:cell denotes e
T6310 7621-7622 CHEBI:51686 denotes x
T6311 7633-7637 SO_EXT:0000704 denotes gene
T6312 7633-7648 GO:0010467 denotes gene expression
T6313 7661-7665 PR_EXT:000006847 denotes ESG1
T6314 7665-7666 SO_EXT:sequence_nullness_or_absence denotes -
T6315 7667-7668 SO_EXT:sequence_nullness_or_absence denotes -
T6316 7669-7677 CL:0002322 denotes ES cells
T6317 7672-7677 CL_GO_EXT:cell denotes cells
T6318 7684-7699 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotide
T6319 7706-7709 CHEBI_SO_EXT:DNA denotes DNA
T6320 7743-7748 SO_EXT:0000704 denotes genes
T6321 7775-7783 CL:0002322 denotes ES cells
T6322 7778-7783 CL_GO_EXT:cell denotes cells
T6323 7785-7789 PR_EXT:000006847 denotes ESG1
T6324 7812-7816 SO_EXT:0000704 denotes gene
T6325 7851-7855 PR_EXT:000006847 denotes ESG1
T6326 7855-7856 SO_EXT:sequence_nullness_or_absence denotes -
T6374 8955-8956 NCBITaxon:10088 denotes e
T6375 8961-8965 PR_EXT:000006847 denotes ESG1
T6376 8965-8966 SO_EXT:sequence_nullness_or_absence denotes -
T6377 8975-8979 NCBITaxon:10088 denotes mice
T6378 8993-8998 GO_EXT:beta_geo denotes β-geo
T6379 8999-9003 PR_EXT:000006847 denotes ESG1
T6380 9003-9004 SO_EXT:normal_or_wild_type_or_present denotes +
T6381 9005-9006 SO_EXT:sequence_nullness_or_absence denotes -
T6382 9007-9014 CL:0002322 denotes ES cell
T6383 9010-9014 CL_GO_EXT:cell denotes cell
T6384 9015-9021 SO_EXT:sequence_cloned_entity denotes clones
T6385 9031-9042 UBERON:0000358 denotes blastocysts
T6386 9053-9057 NCBITaxon:10088 denotes mice
T6387 9104-9110 SO_EXT:sequence_cloned_entity denotes clones
T6388 9124-9128 PR_EXT:000006847 denotes ESG1
T6389 9128-9129 SO_EXT:sequence_nullness_or_absence denotes -
T6390 9130-9131 SO_EXT:sequence_nullness_or_absence denotes -
T6391 9132-9136 NCBITaxon:10088 denotes mice
T6392 9165-9174 SO_EXT:wild_type_entity_or_quality denotes wild-type
T6393 9179-9183 PR_EXT:000006847 denotes ESG1
T6394 9183-9184 SO_EXT:normal_or_wild_type_or_present denotes +
T6395 9185-9186 SO_EXT:sequence_nullness_or_absence denotes -
T6396 9195-9199 PR_EXT:000006847 denotes ESG1
T6397 9199-9200 SO_EXT:sequence_nullness_or_absence denotes -
T6398 9201-9202 SO_EXT:sequence_nullness_or_absence denotes -
T6399 9225-9229 PR_EXT:000006847 denotes ESG1
T6400 9229-9230 SO_EXT:normal_or_wild_type_or_present denotes +
T6402 9233-9237 NCBITaxon:10088 denotes mice
R1328 T6245 T6244 _lexicallyChainedTo genes,fusion of

craft-sa-dev

Id Subject Object Predicate Lexical cue
T4020 24-35 JJ denotes Chromosomal
T4021 36-48 NN denotes localization
T4022 49-52 CC denotes and
T4023 53-63 NNS denotes structures
T4024 64-66 IN denotes of
T4025 67-72 NN denotes mouse
T4026 78-82 NN denotes gene
T4027 73-77 NN denotes ESG1
T4028 83-86 CC denotes and
T4029 87-97 NNS denotes psedogenes
T4030 97-280 sentence denotes To determine the chromosomal localizations of the mouse ESG1 gene and pseudogenes, we performed a Blast analysis of the mouse genomic database with the ESG1 cDNA sequence as a query.
T4031 98-100 TO denotes To
T4032 101-110 VB denotes determine
T4033 184-193 VBD denotes performed
T4034 111-114 DT denotes the
T4035 127-140 NNS denotes localizations
T4036 115-126 JJ denotes chromosomal
T4037 141-143 IN denotes of
T4038 144-147 DT denotes the
T4039 159-163 NN denotes gene
T4040 148-153 NN denotes mouse
T4041 154-158 NN denotes ESG1
T4042 164-167 CC denotes and
T4043 168-179 NNS denotes pseudogenes
T4044 179-181 , denotes ,
T4045 181-183 PRP denotes we
T4046 194-195 DT denotes a
T4047 202-210 NN denotes analysis
T4048 196-201 NNP denotes Blast
T4049 211-213 IN denotes of
T4050 214-217 DT denotes the
T4051 232-240 NN denotes database
T4052 218-223 NN denotes mouse
T4053 224-231 JJ denotes genomic
T4054 241-245 IN denotes with
T4055 246-249 DT denotes the
T4056 260-268 NN denotes sequence
T4057 250-254 NN denotes ESG1
T4058 255-259 NN denotes cDNA
T4059 269-271 IN denotes as
T4060 272-273 DT denotes a
T4061 274-279 NN denotes query
T4062 279-280 . denotes .
T4063 280-398 sentence denotes We identified several putative pseudogenes without introns on chromosomes 1, 5, 11, 12, 14, 16, 17, and X (Figure 1).
T4064 281-283 PRP denotes We
T4065 284-294 VBD denotes identified
T4066 295-302 JJ denotes several
T4067 312-323 NNS denotes pseudogenes
T4068 303-311 JJ denotes putative
T4069 324-331 IN denotes without
T4070 332-339 NNS denotes introns
T4071 340-342 IN denotes on
T4072 343-354 NNS denotes chromosomes
T4073 385-386 NN denotes X
T4074 355-356 CD denotes 1
T4075 356-358 , denotes ,
T4076 358-359 CD denotes 5
T4077 359-361 , denotes ,
T4078 361-363 CD denotes 11
T4079 363-365 , denotes ,
T4080 365-367 CD denotes 12
T4081 367-369 , denotes ,
T4082 369-371 CD denotes 14
T4083 371-373 , denotes ,
T4084 373-375 CD denotes 16
T4085 375-377 , denotes ,
T4086 377-379 CD denotes 17
T4087 379-381 , denotes ,
T4088 381-384 CC denotes and
T4089 387-388 -LRB- denotes (
T4090 388-394 NN denotes Figure
T4091 395-396 CD denotes 1
T4092 396-397 -RRB- denotes )
T4093 397-398 . denotes .
T4094 398-530 sentence denotes In addition, two intron-less pseudogenes were identified in DNA fragments for which the chromosomal localization remained unmapped.
T4122 582-584 IN denotes to
T4123 585-589 NN denotes ESG1
T4124 590-594 NN denotes cDNA
T4125 594-596 , denotes ,
T4126 596-600 PRP denotes they
T4127 601-606 MD denotes could
T4128 607-610 RB denotes not
T4129 619-629 JJ denotes functional
T4130 630-638 NN denotes proteins
T4131 638-640 , denotes ,
T4132 640-647 IN denotes because
T4133 648-650 IN denotes of
T4134 651-659 JJ denotes critical
T4135 660-669 NNS denotes mutations
T4136 669-670 . denotes .
T4137 670-778 sentence denotes This result suggests that there are a larger number of intron-less pseudogenes than previously anticipated.
T4138 671-675 DT denotes This
T4139 676-682 NN denotes result
T4140 683-691 VBZ denotes suggests
T4141 692-696 IN denotes that
T4142 703-706 VBP denotes are
T4143 697-702 EX denotes there
T4144 707-708 DT denotes a
T4145 716-722 NN denotes number
T4146 709-715 JJR denotes larger
T4147 723-725 IN denotes of
T4148 726-737 JJ denotes intron-less
T4149 738-749 NNS denotes pseudogenes
T4150 750-754 IN denotes than
T4151 766-777 VBN denotes anticipated
T4152 755-765 RB denotes previously
T4153 777-778 . denotes .
T4154 778-872 sentence denotes Existence of multiple retropseudogenes is a hallmark of pluripotent cell-specific genes [18].
T4155 779-788 NN denotes Existence
T4156 818-820 VBZ denotes is
T4157 789-791 IN denotes of
T4158 792-800 JJ denotes multiple
T4159 801-817 NNS denotes retropseudogenes
T4160 821-822 DT denotes a
T4161 823-831 NN denotes hallmark
T4162 832-834 IN denotes of
T4163 835-846 JJ denotes pluripotent
T4164 861-866 NNS denotes genes
T4165 847-851 NN denotes cell
T4166 852-860 JJ denotes specific
T4167 851-852 HYPH denotes -
T4168 867-868 -LRB- denotes [
T4169 868-870 CD denotes 18
T4170 870-871 -RRB- denotes ]
T4171 871-872 . denotes .
T4172 872-873 sentence denotes
T4187 1176-1180 NN denotes ESG1
T4188 1186-1190 WDT denotes that
T4189 1191-1199 VBD denotes included
T4190 1200-1203 CD denotes two
T4191 1213-1220 NNS denotes introns
T4192 1204-1212 JJ denotes putative
T4193 1220-1221 . denotes .
T4194 1221-1329 sentence denotes These putative first and second exons, however, contained (4) multiple mutations of the ESG1 cDNA sequence.
T4195 1222-1227 DT denotes These
T4196 1254-1259 NNS denotes exons
T4197 1228-1236 JJ denotes putative
T4198 1237-1242 JJ denotes first
T4199 1243-1246 CC denotes and
T4200 1247-1253 JJ denotes second
T4201 1270-1279 VBD denotes contained
T4202 1259-1261 , denotes ,
T4203 1261-1268 RB denotes however
T4204 1268-1270 , denotes ,
T4205 1280-1281 -LRB- denotes (
T4206 1281-1282 CD denotes 4
T4207 1282-1283 -RRB- denotes )
T4208 1284-1292 JJ denotes multiple
T4209 1293-1302 NNS denotes mutations
T4210 1303-1305 IN denotes of
T4211 1306-1309 DT denotes the
T4212 1320-1328 NN denotes sequence
T4213 1310-1314 NN denotes ESG1
T4214 1315-1319 NN denotes cDNA
T4215 1328-1329 . denotes .
T4216 1329-1413 sentence denotes The putative third exon was identical to that of the previously reported ESG1 gene.
T4217 1330-1333 DT denotes The
T4218 1349-1353 NN denotes exon
T4219 1334-1342 JJ denotes putative
T4220 1343-1348 JJ denotes third
T4221 1354-1357 VBD denotes was
T4222 1358-1367 JJ denotes identical
T4223 1368-1370 IN denotes to
T4224 1371-1375 DT denotes that
T4225 1376-1378 IN denotes of
T4226 1379-1382 DT denotes the
T4227 1408-1412 NN denotes gene
T4228 1383-1393 RB denotes previously
T4229 1394-1402 VBN denotes reported
T4230 1403-1407 NN denotes ESG1
T4231 1412-1413 . denotes .
T4232 1413-1543 sentence denotes Also on chromosome 9, we identified another DNA fragment that was similar, but not identical, to the third exon of the ESG1 gene.
T4233 1414-1418 RB denotes Also
T4234 1439-1449 VBD denotes identified
T4235 1419-1421 IN denotes on
T4236 1422-1432 NN denotes chromosome
T4237 1433-1434 CD denotes 9
T4238 1434-1436 , denotes ,
T4239 1436-1438 PRP denotes we
T4240 1450-1457 DT denotes another
T4241 1462-1470 NN denotes fragment
T4242 1458-1461 NN denotes DNA
T4243 1471-1475 WDT denotes that
T4244 1476-1479 VBD denotes was
T4245 1480-1487 JJ denotes similar
T4246 1487-1489 , denotes ,
T4247 1489-1492 CC denotes but
T4248 1493-1496 RB denotes not
T4249 1497-1506 JJ denotes identical
T4250 1506-1508 , denotes ,
T4251 1508-1510 IN denotes to
T4252 1511-1514 DT denotes the
T4253 1521-1525 NN denotes exon
T4254 1515-1520 JJ denotes third
T4255 1526-1528 IN denotes of
T4256 1529-1532 DT denotes the
T4257 1538-1542 NN denotes gene
T4258 1533-1537 NN denotes ESG1
T4259 1542-1543 . denotes .
T4260 1543-1648 sentence denotes These findings suggest that these ESG1-like sequences on chromosome 9 have not been correctly assembled.
T4261 1544-1549 DT denotes These
T4262 1550-1558 NNS denotes findings
T4263 1559-1566 VBP denotes suggest
T4264 1567-1571 IN denotes that
T4265 1638-1647 VBN denotes assembled
T4266 1572-1577 DT denotes these
T4267 1588-1597 NNS denotes sequences
T4268 1578-1582 NN denotes ESG1
T4269 1583-1587 JJ denotes like
T4270 1582-1583 HYPH denotes -
T4271 1598-1600 IN denotes on
T4272 1601-1611 NN denotes chromosome
T4273 1612-1613 CD denotes 9
T4274 1614-1618 VBP denotes have
T4275 1619-1622 RB denotes not
T4276 1623-1627 VBN denotes been
T4277 1628-1637 RB denotes correctly
T4278 1647-1648 . denotes .
T4279 1648-1847 sentence denotes To obtain DNA fragments containing the ESG1 gene, we screened the bacterial artificial chromosome (BAC) DNA pool by PCR using primer pairs that would only amplify the real gene, not the pseudogenes.
T4280 1649-1651 TO denotes To
T4281 1652-1658 VB denotes obtain
T4282 1702-1710 VBD denotes screened
T4283 1659-1662 NN denotes DNA
T4284 1663-1672 NNS denotes fragments
T4285 1673-1683 VBG denotes containing
T4286 1684-1687 DT denotes the
T4287 1693-1697 NN denotes gene
T4288 1688-1692 NN denotes ESG1
T4289 1697-1699 , denotes ,
T4290 1699-1701 PRP denotes we
T4291 1711-1714 DT denotes the
T4292 1757-1761 NN denotes pool
T4293 1715-1724 JJ denotes bacterial
T4294 1736-1746 NN denotes chromosome
T4295 1725-1735 JJ denotes artificial
T4296 1747-1748 -LRB- denotes (
T4297 1748-1751 NN denotes BAC
T4298 1751-1752 -RRB- denotes )
T4299 1753-1756 NN denotes DNA
T4300 1762-1764 IN denotes by
T4301 1765-1768 NN denotes PCR
T4302 1769-1774 VBG denotes using
T4303 1775-1781 NN denotes primer
T4304 1782-1787 NNS denotes pairs
T4305 1788-1792 WDT denotes that
T4306 1804-1811 VB denotes amplify
T4307 1793-1798 MD denotes would
T4308 1799-1803 RB denotes only
T4309 1812-1815 DT denotes the
T4310 1821-1825 NN denotes gene
T4311 1816-1820 JJ denotes real
T4312 1825-1827 , denotes ,
T4313 1827-1830 RB denotes not
T4314 1835-1846 NNS denotes pseudogenes
T4315 1831-1834 DT denotes the
T4316 1846-1847 . denotes .
T4317 1847-1904 sentence denotes We obtained two independent, but overlapping BAC clones.
T4318 1848-1850 PRP denotes We
T4319 1851-1859 VBD denotes obtained
T4320 1860-1863 CD denotes two
T4321 1897-1903 NNS denotes clones
T4322 1864-1875 JJ denotes independent
T4323 1875-1877 , denotes ,
T4324 1877-1880 CC denotes but
T4325 1881-1892 VBG denotes overlapping
T4326 1893-1896 NN denotes BAC
T4327 1903-1904 . denotes .
T4328 1904-2096 sentence denotes Southern blot analyses and sequencing demonstrated that these clones contained a sequence exhibiting complete identity with ESG1 cDNA that was interrupted by two putative introns (Figure 2A).
T4329 1905-1913 NNP denotes Southern
T4330 1914-1918 NN denotes blot
T4331 1943-1955 VBD denotes demonstrated
T4332 1919-1927 NNS denotes analyses
T4333 1928-1931 CC denotes and
T4334 1932-1942 NN denotes sequencing
T4335 1956-1960 IN denotes that
T4336 1974-1983 VBD denotes contained
T4337 1961-1966 DT denotes these
T4338 1967-1973 NNS denotes clones
T4339 1984-1985 DT denotes a
T4340 1986-1994 NN denotes sequence
T4341 1995-2005 VBG denotes exhibiting
T4342 2006-2014 JJ denotes complete
T4343 2015-2023 NN denotes identity
T4344 2024-2028 IN denotes with
T4345 2029-2033 NN denotes ESG1
T4346 2034-2038 NN denotes cDNA
T4347 2039-2043 WDT denotes that
T4348 2048-2059 VBN denotes interrupted
T4349 2044-2047 VBD denotes was
T4350 2060-2062 IN denotes by
T4351 2063-2066 CD denotes two
T4352 2076-2083 NNS denotes introns
T4353 2067-2075 JJ denotes putative
T4354 2084-2085 -LRB- denotes (
T4355 2092-2094 NN denotes 2A
T4356 2085-2091 NN denotes Figure
T4357 2094-2095 -RRB- denotes )
T4358 2095-2096 . denotes .
T4359 2096-2215 sentence denotes The two intron sequences begin with GT and terminate with AG, fulfilling the GT-AG rule of exon-intron junctions [19].
T4360 2097-2100 DT denotes The
T4361 2112-2121 NNS denotes sequences
T4362 2101-2104 CD denotes two
T4363 2105-2111 NN denotes intron
T4364 2122-2127 VBP denotes begin
T4365 2128-2132 IN denotes with
T4366 2133-2135 NN denotes GT
T4367 2136-2139 CC denotes and
T4368 2140-2149 VB denotes terminate
T4369 2150-2154 IN denotes with
T4395 2242-2246 DT denotes this
T4396 2251-2259 NN denotes fragment
T4397 2247-2250 NN denotes DNA
T4398 2270-2276 JJ denotes strong
T4399 2295-2303 NN denotes activity
T4400 2277-2285 NN denotes promoter
T4401 2286-2294 NN denotes enhancer
T4402 2285-2286 HYPH denotes /
T4403 2304-2306 IN denotes by
T4404 2307-2317 NN denotes luciferase
T4405 2327-2333 NNS denotes assays
T4406 2318-2326 NN denotes reporter
T4407 2334-2336 IN denotes in
T4408 2337-2353 JJ denotes undifferentiated
T4409 2357-2362 NNS denotes cells
T4410 2354-2356 NN denotes ES
T4411 2362-2364 , denotes ,
T4412 2364-2367 CC denotes but
T4413 2368-2371 RB denotes not
T4414 2372-2374 IN denotes in
T4415 2375-2382 JJ denotes somatic
T4416 2383-2388 NNS denotes cells
T4417 2389-2390 -LRB- denotes (
T4418 2390-2396 NN denotes Figure
T4419 2397-2398 CD denotes 3
T4420 2398-2399 -RRB- denotes )
T4421 2399-2400 . denotes .
T4422 2400-2499 sentence denotes The same fragment showed much weaker activity after induction of differentiation by retinoic acid.
T4423 2401-2404 DT denotes The
T4424 2410-2418 NN denotes fragment
T4425 2405-2409 JJ denotes same
T4426 2419-2425 VBD denotes showed
T4427 2426-2430 RB denotes much
T4428 2431-2437 JJR denotes weaker
T4429 2438-2446 NN denotes activity
T4430 2447-2452 IN denotes after
T4431 2453-2462 NN denotes induction
T4432 2463-2465 IN denotes of
T4433 2466-2481 NN denotes differentiation
T4434 2482-2484 IN denotes by
T4435 2485-2493 JJ denotes retinoic
T4436 2494-2498 NN denotes acid
T4437 2498-2499 . denotes .
T4438 2499-2549 sentence denotes We concluded that this sequence is the ESG1 gene.
T4439 2500-2502 PRP denotes We
T4440 2503-2512 VBD denotes concluded
T4441 2513-2517 IN denotes that
T4442 2532-2534 VBZ denotes is
T4443 2518-2522 DT denotes this
T4444 2523-2531 NN denotes sequence
T4445 2535-2538 DT denotes the
T4446 2544-2548 NN denotes gene
T4447 2539-2543 NN denotes ESG1
T4448 2548-2549 . denotes .
T4487 3587-3588 . denotes .
T4488 3588-3690 sentence denotes The ESG1-like sequence exhibited greater than 95% identity to the exons and introns of the ESG1 gene.
T4489 3589-3592 DT denotes The
T4490 3603-3611 NN denotes sequence
T4491 3593-3597 NN denotes ESG1
T4492 3598-3602 JJ denotes like
T4493 3597-3598 HYPH denotes -
T4494 3612-3621 VBD denotes exhibited
T4495 3622-3629 JJR denotes greater
T4496 3635-3637 CD denotes 95
T4497 3630-3634 IN denotes than
T4498 3637-3638 NN denotes %
T4499 3639-3647 NN denotes identity
T4500 3648-3650 IN denotes to
T4501 3651-3654 DT denotes the
T4502 3655-3660 NNS denotes exons
T4503 3661-3664 CC denotes and
T4504 3665-3672 NNS denotes introns
T4505 3673-3675 IN denotes of
T4506 3676-3679 DT denotes the
T4507 3685-3689 NN denotes gene
T4508 3680-3684 NN denotes ESG1
T4509 3689-3690 . denotes .
T4510 3690-3830 sentence denotes This sequence, however, contained critical nucleotide substitutions in all of the exons and one nucleotide insertion in exon 2 (Figure 2B).
T4511 3691-3695 DT denotes This
T4512 3696-3704 NN denotes sequence
T4513 3715-3724 VBD denotes contained
T4514 3704-3706 , denotes ,
T4515 3706-3713 RB denotes however
T4516 3713-3715 , denotes ,
T4517 3725-3733 JJ denotes critical
T4518 3745-3758 NNS denotes substitutions
T4519 3734-3744 NN denotes nucleotide
T4520 3759-3761 IN denotes in
T4521 3762-3765 DT denotes all
T4522 3766-3768 IN denotes of
T4523 3769-3772 DT denotes the
T4524 3773-3778 NNS denotes exons
T4525 3779-3782 CC denotes and
T4526 3783-3786 CD denotes one
T4527 3798-3807 NN denotes insertion
T4528 3787-3797 NN denotes nucleotide
T4529 3808-3810 IN denotes in
T4530 3811-3815 NN denotes exon
T4531 3816-3817 CD denotes 2
T4532 3818-3819 -LRB- denotes (
T4533 3826-3828 NN denotes 2B
T4534 3819-3825 NN denotes Figure
T4535 3828-3829 -RRB- denotes )
T4536 3829-3830 . denotes .
T4537 3830-4001 sentence denotes Although 675 base pairs of the 3' flanking regions were conserved between the ESG1 gene and the pseudogene, only five base pairs of the 5' flanking region were identical.
T4538 3831-3839 IN denotes Although
T4539 3887-3896 VBN denotes conserved
T4540 3840-3843 CD denotes 675
T4541 3849-3854 NNS denotes pairs
T4542 3844-3848 NN denotes base
T4543 3855-3857 IN denotes of
T4544 3858-3861 DT denotes the
T4545 3874-3881 NNS denotes regions
T4546 3862-3863 CD denotes 3
T4547 3863-3864 SYM denotes '
T4548 3865-3873 NN denotes flanking
T4549 3882-3886 VBD denotes were
T4550 3986-3990 VBD denotes were
T4551 3897-3904 IN denotes between
T4552 3905-3908 DT denotes the
T4553 3914-3918 NN denotes gene
T4554 3909-3913 NN denotes ESG1
T4555 3919-3922 CC denotes and
T4556 3923-3926 DT denotes the
T4557 3927-3937 NN denotes pseudogene
T4558 3937-3939 , denotes ,
T4559 3939-3943 RB denotes only
T4560 3954-3959 NNS denotes pairs
T4561 3944-3948 CD denotes five
T4562 3949-3953 NN denotes base
T4563 3960-3962 IN denotes of
T4564 3963-3966 DT denotes the
T4565 3979-3985 NN denotes region
T4566 3967-3968 CD denotes 5
T4567 3968-3969 SYM denotes '
T4568 3970-3978 NN denotes flanking
T4569 3991-4000 JJ denotes identical
T4570 4000-4001 . denotes .
T4571 4001-4123 sentence denotes This 5' flanking region (~6 kbp) did not possess any promoter/enhancer activity in luciferase reporter assays (Figure 3).
T4572 4002-4006 DT denotes This
T4573 4019-4025 NN denotes region
T4574 4007-4008 CD denotes 5
T4575 4008-4009 SYM denotes '
T4576 4010-4018 NN denotes flanking
T4577 4043-4050 VB denotes possess
T4578 4026-4027 -LRB- denotes (
T4579 4030-4033 NN denotes kbp
T4580 4027-4028 SYM denotes ~
T4581 4028-4029 CD denotes 6
T4582 4033-4034 -RRB- denotes )
T4583 4035-4038 VBD denotes did
T4584 4039-4042 RB denotes not
T4585 4051-4054 DT denotes any
T4586 4073-4081 NN denotes activity
T4587 4055-4063 NN denotes promoter
T4588 4064-4072 NN denotes enhancer
T4589 4063-4064 HYPH denotes /
T4590 4082-4084 IN denotes in
T4591 4085-4095 NN denotes luciferase
T4592 4096-4104 NN denotes reporter
T4593 4105-4111 NNS denotes assays
T4594 4112-4113 -LRB- denotes (
T4595 4113-4119 NN denotes Figure
T4596 4120-4121 CD denotes 3
T4597 4121-4122 -RRB- denotes )
T4598 4122-4123 . denotes .
T4599 4123-4218 sentence denotes It is thus unlikely that this sequence is transcribed or translated into a functional protein.
T4600 4124-4126 PRP denotes It
T4601 4127-4129 VBZ denotes is
T4602 4130-4134 RB denotes thus
T4603 4135-4143 JJ denotes unlikely
T4604 4144-4148 IN denotes that
T4605 4166-4177 VBN denotes transcribed
T4606 4149-4153 DT denotes this
T4607 4154-4162 NN denotes sequence
T4608 4163-4165 VBZ denotes is
T4609 4178-4180 CC denotes or
T4610 4181-4191 VBN denotes translated
T4611 4192-4196 IN denotes into
T4612 4197-4198 DT denotes a
T4613 4210-4217 NN denotes protein
T4614 4199-4209 JJ denotes functional
T4615 4217-4218 . denotes .
T4616 4218-4276 sentence denotes This sequence likely represents a duplication pseudogene.
T4617 4219-4223 DT denotes This
T4618 4224-4232 NN denotes sequence
T4619 4240-4250 VBZ denotes represents
T4620 4233-4239 RB denotes likely
T4621 4251-4252 DT denotes a
T4622 4265-4275 NN denotes pseudogene
T4623 4253-4264 NN denotes duplication
T4624 4275-4276 . denotes .
T4625 4276-4399 sentence denotes Bierbaum previously reported the existence of two pseudogenes with similar exon-intron organization as the ESG1 gene [17].
T4626 4277-4285 NN denotes Bierbaum
T4627 4297-4305 VBD denotes reported
T4628 4286-4296 RB denotes previously
T4629 4306-4309 DT denotes the
T4630 4310-4319 NN denotes existence
T4631 4320-4322 IN denotes of
T4632 4323-4326 CD denotes two
T4633 4327-4338 NNS denotes pseudogenes
T4634 4339-4343 IN denotes with
T4635 4344-4351 JJ denotes similar
T4636 4364-4376 NN denotes organization
T4637 4352-4356 NN denotes exon
T4638 4357-4363 NN denotes intron
T4639 4356-4357 HYPH denotes -
T4640 4377-4379 IN denotes as
T4641 4380-4383 DT denotes the
T4642 4389-4393 NN denotes gene
T4643 4384-4388 NN denotes ESG1
T4644 4394-4395 -LRB- denotes [
T4645 4395-4397 CD denotes 17
T4646 4397-4398 -RRB- denotes ]
T4647 4398-4399 . denotes .
T4648 4399-4535 sentence denotes We could not determine which of these two pseudogenes corresponds to the one we identified or the location of the remaining pseudogene.
T4649 4400-4402 PRP denotes We
T4650 4413-4422 VB denotes determine
T4651 4403-4408 MD denotes could
T4652 4409-4412 RB denotes not
T4653 4423-4428 WDT denotes which
T4654 4454-4465 VBZ denotes corresponds
T4655 4429-4431 IN denotes of
T4656 4432-4437 DT denotes these
T4657 4442-4453 NNS denotes pseudogenes
T4658 4438-4441 CD denotes two
T4659 4466-4468 IN denotes to
T4660 4469-4472 DT denotes the
T4661 4473-4476 CD denotes one
T4662 4477-4479 PRP denotes we
T4663 4480-4490 VBD denotes identified
T4664 4491-4493 CC denotes or
T4665 4494-4497 DT denotes the
T4666 4498-4506 NN denotes location
T4667 4507-4509 IN denotes of
T4668 4510-4513 DT denotes the
T4669 4524-4534 NN denotes pseudogene
T4670 4514-4523 VBG denotes remaining
T4671 4534-4535 . denotes .
T6437 4537-4545 VBN denotes Targeted
T6438 4546-4556 NN denotes disruption
T6439 4557-4559 IN denotes of
T6440 4560-4563 DT denotes the
T6441 4575-4579 NN denotes gene
T6442 4564-4569 NN denotes mouse
T6443 4570-4574 NN denotes ESG1
T6444 4579-4677 sentence denotes To study the function of ESG1, we deleted the gene by homologous recombination in mouse ES cells.
T6445 4580-4582 TO denotes To
T6446 4583-4588 VB denotes study
T6447 4614-4621 VBD denotes deleted
T6448 4589-4592 DT denotes the
T6449 4593-4601 NN denotes function
T6450 4602-4604 IN denotes of
T6451 4605-4609 NN denotes ESG1
T6452 4609-4611 , denotes ,
T6453 4611-4613 PRP denotes we
T6454 4622-4625 DT denotes the
T6455 4626-4630 NN denotes gene
T6456 4631-4633 IN denotes by
T6457 4634-4644 JJ denotes homologous
T6458 4645-4658 NN denotes recombination
T6459 4659-4661 IN denotes in
T6460 4662-4667 NN denotes mouse
T6461 4671-4676 NNS denotes cells
T6462 4668-4670 NN denotes ES
T6463 4676-4677 . denotes .
T6464 4677-4916 sentence denotes We replaced the three exons with either a fusion of the neomycin-resistance and β-galactosidase genes (β-geo) or the hygromycin resistant gene (HygR) using two targeting vectors (Figure 4A) introduced into RF8 ES cells by electroporation.
T6465 4678-4680 PRP denotes We
T6466 4681-4689 VBD denotes replaced
T6467 4690-4693 DT denotes the
T6468 4700-4705 NNS denotes exons
T6469 4694-4699 CD denotes three
T6470 4706-4710 IN denotes with
T6471 4711-4717 CC denotes either
T6472 4720-4726 NN denotes fusion
T6473 4718-4719 DT denotes a
T6474 4727-4729 IN denotes of
T6475 4730-4733 DT denotes the
T6476 4774-4779 NNS denotes genes
T6477 4734-4742 NN denotes neomycin
T6478 4743-4753 NN denotes resistance
T6479 4742-4743 HYPH denotes -
T6480 4754-4757 CC denotes and
T6481 4758-4759 NN denotes β
T6482 4760-4773 NN denotes galactosidase
T6483 4759-4760 HYPH denotes -
T6484 4780-4781 -LRB- denotes (
T6485 4783-4786 NN denotes geo
T6486 4781-4782 NN denotes β
T6487 4782-4783 HYPH denotes -
T6488 4786-4787 -RRB- denotes )
T6489 4788-4790 CC denotes or
T6490 4791-4794 DT denotes the
T6491 4816-4820 NN denotes gene
T6517 4943-4949 NNS denotes clones
T6518 4935-4937 NN denotes ES
T6519 4938-4942 NN denotes cell
T6520 4950-4954 IN denotes with
T6521 4955-4962 JJ denotes correct
T6522 4974-4987 NN denotes recombination
T6523 4963-4973 JJ denotes homologous
T6524 4988-4990 IN denotes of
T6525 4991-4994 DT denotes the
T6526 5011-5017 NN denotes vector
T6527 4995-4996 NN denotes β
T6528 4997-5000 NN denotes geo
T6529 4996-4997 HYPH denotes -
T6530 5001-5010 NN denotes targeting
T6531 5017-5019 , denotes ,
T6532 5019-5024 WDT denotes which
T6533 5029-5038 VBN denotes confirmed
T6534 5025-5028 VBD denotes was
T6535 5039-5041 IN denotes by
T6536 5042-5050 NNP denotes Southern
T6537 5051-5055 NN denotes blot
T6538 5056-5064 NN denotes analysis
T6539 5065-5066 -LRB- denotes (
T6540 5073-5075 NN denotes 4B
T6541 5066-5072 NN denotes Figure
T6542 5075-5076 -RRB- denotes )
T6543 5076-5077 . denotes .
T6544 5077-5172 sentence denotes We obtained only one clone with correct homologous recombination of the HygR targeting vector.
T6545 5078-5080 PRP denotes We
T6546 5081-5089 VBD denotes obtained
T6547 5090-5094 RB denotes only
T6548 5099-5104 NN denotes clone
T6549 5095-5098 CD denotes one
T6550 5105-5109 IN denotes with
T6551 5110-5117 JJ denotes correct
T6552 5129-5142 NN denotes recombination
T6553 5118-5128 JJ denotes homologous
T6554 5143-5145 IN denotes of
T6555 5146-5149 DT denotes the
T6556 5165-5171 NN denotes vector
T6557 5150-5154 NN denotes HygR
T6558 5155-5164 NN denotes targeting
T6559 5171-5172 . denotes .
T6560 5172-5173 sentence denotes
T6561 6048-6052 sentence denotes To o
T6562 6048-6049 VBN denotes T
T6563 6049-6050 NN denotes o
T6564 6051-6052 IN denotes o
T6565 6052-6054 sentence denotes bt
T6566 6052-6053 IN denotes b
T6567 6053-6054 JJ denotes t
T6568 6054-6060 sentence denotes ain ho
T6569 6058-6060 VBN denotes ho
T6570 6054-6055 CC denotes a
T6611 6187-6189 , denotes ,
T6612 6189-6191 CD denotes 49
T6613 6197-6207 JJ denotes homozygous
T6614 6208-6211 IN denotes for
T6615 6212-6216 NN denotes ESG1
T6616 6217-6225 NN denotes deletion
T6617 6225-6226 . denotes .
T6618 6226-6324 sentence denotes Northern blot and western blot analyses confirmed the absence of ESG1 in these cells (Figure 4C).
T6619 6227-6235 NNP denotes Northern
T6620 6236-6240 NN denotes blot
T6621 6258-6266 NNS denotes analyses
T6622 6241-6244 CC denotes and
T6623 6245-6252 NNP denotes western
T6624 6253-6257 NN denotes blot
T6625 6267-6276 VBD denotes confirmed
T6626 6277-6280 DT denotes the
T6627 6281-6288 NN denotes absence
T6628 6289-6291 IN denotes of
T6629 6292-6296 NN denotes ESG1
T6630 6297-6299 IN denotes in
T6631 6300-6305 DT denotes these
T6632 6306-6311 NNS denotes cells
T6633 6312-6313 -LRB- denotes (
T6634 6320-6322 NN denotes 4C
T6635 6313-6319 NN denotes Figure
T6636 6322-6323 -RRB- denotes )
T6637 6323-6324 . denotes .
T6638 6324-6428 sentence denotes In 29 clones, the β-geo vector had replaced the HygR vector, such that the cells remained heterozygous.
T6639 6325-6327 IN denotes In
T6640 6360-6368 VBN denotes replaced
T6641 6328-6330 CD denotes 29
T6642 6331-6337 NNS denotes clones
T6643 6337-6339 , denotes ,
T6644 6339-6342 DT denotes the
T6645 6349-6355 NN denotes vector
T6646 6343-6344 NN denotes β
T6647 6345-6348 NN denotes geo
T6648 6344-6345 HYPH denotes -
T6649 6356-6359 VBD denotes had
T6650 6369-6372 DT denotes the
T6651 6378-6384 NN denotes vector
T6652 6373-6377 NN denotes HygR
T6653 6384-6386 , denotes ,
T6654 6386-6390 JJ denotes such
T6655 6406-6414 VBD denotes remained
T6656 6391-6395 IN denotes that
T6657 6396-6399 DT denotes the
T6658 6400-6405 NNS denotes cells
T6659 6415-6427 JJ denotes heterozygous
T6660 6427-6428 . denotes .
T6661 6428-6513 sentence denotes In the remaining 27 clones, the β-geo vector was integrated at non-homologous sites.
T6662 6429-6431 IN denotes In
T6663 6478-6488 VBN denotes integrated
T6664 6432-6435 DT denotes the
T6665 6449-6455 NNS denotes clones
T6666 6436-6445 VBG denotes remaining
T6667 6446-6448 CD denotes 27
T6668 6455-6457 , denotes ,
T6669 6457-6460 DT denotes the
T6670 6467-6473 NN denotes vector
T6671 6461-6462 NN denotes β
T6672 6463-6466 NN denotes geo
T6673 6462-6463 HYPH denotes -
T6674 6474-6477 VBD denotes was
T6675 6489-6491 IN denotes at
T6676 6492-6506 JJ denotes non-homologous
T6677 6507-6512 NNS denotes sites
T6678 6512-6513 . denotes .
T6679 6513-6571 sentence denotes ESG1-/- ES cells exhibited normal morphology (Figure 5A).
T6680 6514-6518 NN denotes ESG1
T6681 6525-6530 NNS denotes cells
T6682 6518-6519 SYM denotes -
T6683 6519-6520 HYPH denotes /
T6684 6520-6521 SYM denotes -
T6685 6522-6524 NN denotes ES
T6686 6531-6540 VBD denotes exhibited
T6687 6541-6547 JJ denotes normal
T6688 6548-6558 NN denotes morphology
T6689 6559-6560 -LRB- denotes (
T6690 6567-6569 NN denotes 5A
T6691 6560-6566 NN denotes Figure
T6692 6569-6570 -RRB- denotes )
T6693 6570-6571 . denotes .
T6694 6571-6694 sentence denotes These cells also proliferated at a speed comparable to that of the control (heterozygous and wild-type) cells (Figure 5B).
T6695 6572-6577 DT denotes These
T6696 6578-6583 NNS denotes cells
T6697 6589-6601 VBD denotes proliferated
T6698 6584-6588 RB denotes also
T6699 6602-6604 IN denotes at
T6700 6605-6606 DT denotes a
T6701 6607-6612 NN denotes speed
T6702 6613-6623 JJ denotes comparable
T6703 6624-6626 IN denotes to
T6704 6627-6631 DT denotes that
T6705 6632-6634 IN denotes of
T6706 6635-6638 DT denotes the
T6707 6676-6681 NNS denotes cells
T6708 6639-6646 NN denotes control
T6709 6647-6648 -LRB- denotes (
T6710 6648-6660 JJ denotes heterozygous
T6711 6661-6664 CC denotes and
T6712 6665-6669 JJ denotes wild
T6713 6670-6674 NN denotes type
T6714 6669-6670 HYPH denotes -
T6715 6674-6675 -RRB- denotes )
T6738 6782-6788 NN denotes Figure
T6739 6791-6792 -RRB- denotes )
T6740 6793-6795 CC denotes or
T6741 6796-6804 JJ denotes retinoic
T6742 6810-6819 NN denotes treatment
T6743 6805-6809 NN denotes acid
T6744 6820-6821 -LRB- denotes (
T6745 6825-6830 VBN denotes shown
T6746 6821-6824 RB denotes not
T6747 6830-6831 -RRB- denotes )
T6748 6831-6832 . denotes .
T6749 6832-6980 sentence denotes When transplanted into hind flanks of nude mice, these cells produced teratomas, tumors containing components of all three germ layers (Figure 5C).
T6750 6833-6837 WRB denotes When
T6751 6838-6850 VBN denotes transplanted
T6752 6894-6902 VBD denotes produced
T6753 6851-6855 IN denotes into
T6754 6856-6860 NN denotes hind
T6755 6861-6867 NNS denotes flanks
T6756 6868-6870 IN denotes of
T6757 6871-6875 JJ denotes nude
T6758 6876-6880 NNS denotes mice
T6759 6880-6882 , denotes ,
T6760 6882-6887 DT denotes these
T6761 6888-6893 NNS denotes cells
T6762 6903-6912 NNS denotes teratomas
T6763 6912-6914 , denotes ,
T6764 6914-6920 NNS denotes tumors
T6765 6921-6931 VBG denotes containing
T6766 6932-6942 NNS denotes components
T6767 6943-6945 IN denotes of
T6768 6946-6949 DT denotes all
T6769 6961-6967 NNS denotes layers
T6770 6950-6955 CD denotes three
T6771 6956-6960 NN denotes germ
T6772 6968-6969 -LRB- denotes (
T6773 6976-6978 NN denotes 5C
T6774 6969-6975 NN denotes Figure
T6775 6978-6979 -RRB- denotes )
T6776 6979-6980 . denotes .
T6777 6980-7090 sentence denotes These results indicate that ESG1 is dispensable for the self-renewal properties and pluripotency of ES cells.
T6778 6981-6986 DT denotes These
T6779 6987-6994 NNS denotes results
T6780 6995-7003 VBP denotes indicate
T6781 7004-7008 IN denotes that
T6782 7014-7016 VBZ denotes is
T6783 7009-7013 NN denotes ESG1
T6784 7017-7028 JJ denotes dispensable
T6785 7029-7032 IN denotes for
T6786 7033-7036 DT denotes the
T6787 7050-7060 NNS denotes properties
T6788 7037-7041 NN denotes self
T6789 7042-7049 NN denotes renewal
T6790 7041-7042 HYPH denotes -
T6791 7061-7064 CC denotes and
T6792 7065-7077 NN denotes pluripotency
T6793 7078-7080 IN denotes of
T6794 7081-7083 NN denotes ES
T6795 7084-7089 NNS denotes cells
T6796 7089-7090 . denotes .
T6797 7090-7091 sentence denotes
T6815 7666-7667 HYPH denotes /
T6816 7667-7668 SYM denotes -
T6817 7669-7671 NN denotes ES
T6818 7678-7683 VBG denotes using
T6819 7684-7699 NN denotes oligonucleotide
T6820 7700-7705 VBN denotes based
T6821 7699-7700 HYPH denotes -
T6822 7710-7721 NNS denotes microarrays
T6823 7706-7709 NN denotes DNA
T6824 7722-7734 VBG denotes representing
T6825 7735-7736 SYM denotes ~
T6826 7736-7742 CD denotes 20,000
T6827 7743-7748 NNS denotes genes
T6828 7748-7749 . denotes .
T6829 7749-7880 sentence denotes In comparison to control ES cells, ESG1 was identified as the gene reduced to the greatest extent in ESG1-/- ES cells (Figure 6A).
T6830 7750-7752 IN denotes In
T6831 7794-7804 VBN denotes identified
T6832 7753-7763 NN denotes comparison
T6833 7764-7766 IN denotes to
T6834 7767-7774 NN denotes control
T6835 7778-7783 NNS denotes cells
T6836 7775-7777 NN denotes ES
T6837 7783-7785 , denotes ,
T6838 7785-7789 NN denotes ESG1
T6839 7790-7793 VBD denotes was
T6840 7805-7807 IN denotes as
T6841 7808-7811 DT denotes the
T6842 7812-7816 NN denotes gene
T6843 7817-7824 VBN denotes reduced
T6844 7825-7827 IN denotes to
T6845 7828-7831 DT denotes the
T6846 7841-7847 NN denotes extent
T6847 7832-7840 JJS denotes greatest
T6848 7848-7850 IN denotes in
T6849 7851-7855 NN denotes ESG1
T6850 7862-7867 NNS denotes cells
T6851 7855-7856 SYM denotes -
T6852 7856-7857 HYPH denotes /
T6853 7857-7858 SYM denotes -
T6854 7859-7861 NN denotes ES
T6855 7868-7869 -LRB- denotes (
T6856 7876-7878 NN denotes 6A
T6857 7869-7875 NN denotes Figure
T6858 7878-7879 -RRB- denotes )
T6859 7879-7880 . denotes .
T6860 7880-7978 sentence denotes The expression of ES cell marker genes, such as Nanog and Oct3/4, was normal in ESG1-/- ES cells.
T6861 7881-7884 DT denotes The
T6862 7885-7895 NN denotes expression
T6863 7947-7950 VBD denotes was
T6864 7896-7898 IN denotes of
T6865 7899-7901 NN denotes ES
T6866 7902-7906 NN denotes cell
T6867 7914-7919 NNS denotes genes
T6868 7907-7913 NN denotes marker
T6869 7919-7921 , denotes ,
T6870 7921-7925 JJ denotes such
T6871 7926-7928 IN denotes as
T6872 7929-7934 NN denotes Nanog
T6873 7935-7938 CC denotes and
T6874 7939-7943 NN denotes Oct3
T6875 7943-7944 HYPH denotes /
T6876 7944-7945 CD denotes 4
T6877 7945-7947 , denotes ,
T6878 7951-7957 JJ denotes normal
T6879 7958-7960 IN denotes in
T6880 7961-7965 NN denotes ESG1
T6881 7972-7977 NNS denotes cells
T6882 7965-7966 SYM denotes -
T6883 7966-7967 HYPH denotes /
T6884 7967-7968 SYM denotes -
T6885 7969-7971 NN denotes ES
T6886 7977-7978 . denotes .
T6887 7978-8063 sentence denotes We confirmed normal Oct3/4 expression at protein levels by Western blot (Figure 6B).
T6888 7979-7981 PRP denotes We
T6889 7982-7991 VBD denotes confirmed
T6890 7992-7998 JJ denotes normal
T6891 8006-8016 NN denotes expression
T6892 7999-8003 NN denotes Oct3
T6893 8003-8004 HYPH denotes /
T6894 8004-8005 CD denotes 4
T6895 8017-8019 IN denotes at
T6897 8028-8034 NNS denotes levels
T6898 8035-8037 IN denotes by
T6899 8038-8045 NNP denotes Western
T6900 8046-8050 NN denotes blot
T6901 8051-8052 -LRB- denotes (
T6902 8059-8061 NN denotes 6B
T6903 8052-8058 NN denotes Figure
T6904 8061-8062 -RRB- denotes )
T6905 8062-8063 . denotes .
T6906 8063-8160 sentence denotes The overall gene expression profiles were similar between control ES cells and ESG1-/- ES cells.
T6907 8064-8067 DT denotes The
T6908 8092-8100 NNS denotes profiles
T6909 8068-8075 JJ denotes overall
T6910 8076-8080 NN denotes gene
T6911 8081-8091 NN denotes expression
T6912 8101-8105 VBD denotes were
T6913 8106-8113 JJ denotes similar
T6914 8114-8121 IN denotes between
T6915 8122-8129 NN denotes control
T6916 8133-8138 NNS denotes cells
T6917 8130-8132 NN denotes ES
T6918 8139-8142 CC denotes and
T6919 8143-8147 NN denotes ESG1
T6920 8154-8159 NNS denotes cells
T6921 8147-8148 SYM denotes -
T6922 8148-8149 HYPH denotes /
T6923 8149-8150 SYM denotes -
T6924 8151-8153 NN denotes ES
T6925 8159-8160 . denotes .
T6926 8160-8288 sentence denotes Several genes exhibited a greater than two-fold reduction in ESG1-/- cells, including Krt1-8, Pem, Ctgf, Ptgs2, Igf2 and Inhba.
T6927 8161-8168 JJ denotes Several
T6928 8169-8174 NNS denotes genes
T6929 8175-8184 VBD denotes exhibited
T6930 8185-8186 DT denotes a
T6931 8209-8218 NN denotes reduction
T6932 8187-8194 JJR denotes greater
T6933 8200-8208 JJ denotes two-fold
T6934 8195-8199 IN denotes than
T6935 8219-8221 IN denotes in
T6936 8222-8226 NN denotes ESG1
T6937 8230-8235 NNS denotes cells
T6938 8226-8227 SYM denotes -
T6939 8227-8228 HYPH denotes /
T6940 8228-8229 SYM denotes -
T6941 8235-8237 , denotes ,
T6942 8237-8246 VBG denotes including
T6943 8247-8251 NN denotes Krt1
T6944 8251-8252 HYPH denotes -
T6945 8252-8253 CD denotes 8
T6946 8253-8255 , denotes ,
T6947 8255-8258 NN denotes Pem
T6948 8258-8260 , denotes ,
T6949 8260-8264 NN denotes Ctgf
T6950 8264-8266 , denotes ,
T6951 8266-8271 NN denotes Ptgs2
T6952 8271-8273 , denotes ,
T6953 8273-8277 NN denotes Igf2
T6954 8278-8281 CC denotes and
T6955 8282-8287 NN denotes Inhba
T6956 8287-8288 . denotes .
T6957 8288-8351 sentence denotes These genes might be regulated directly or indirectly by ESG1.
T6958 8289-8294 DT denotes These
T6959 8295-8300 NNS denotes genes
T6960 8310-8319 VBN denotes regulated
T6961 8301-8306 MD denotes might
T6962 8307-8309 VB denotes be
T6963 8320-8328 RB denotes directly
T6964 8329-8331 CC denotes or
T6965 8332-8342 RB denotes indirectly
T6966 8343-8345 IN denotes by
T6967 8346-8350 NN denotes ESG1
T6968 8350-8351 . denotes .
T6969 8351-8439 sentence denotes Since ESG1 contains a KH-type RNA-binding domain, it may stabilize mRNA of these genes.
T6970 8352-8357 IN denotes Since
T6971 8363-8371 VBZ denotes contains
T6972 8358-8362 NN denotes ESG1
T6973 8409-8418 VB denotes stabilize
T6974 8372-8373 DT denotes a
T6975 8394-8400 NN denotes domain
T6976 8374-8376 NN denotes KH
T6977 8377-8381 NN denotes type
T6978 8376-8377 HYPH denotes -
T6979 8382-8385 NN denotes RNA
T6980 8386-8393 VBG denotes binding
T6981 8385-8386 HYPH denotes -
T6982 8400-8402 , denotes ,
T6983 8402-8404 PRP denotes it
T6984 8405-8408 MD denotes may
T6985 8419-8423 NN denotes mRNA
T6986 8424-8426 IN denotes of
T6987 8427-8432 DT denotes these
T6988 8433-8438 NNS denotes genes
T6989 8438-8439 . denotes .
T6990 8439-8497 sentence denotes Further studied are required to clarify this possibility.
T6991 8440-8447 RB denotes Further
T6992 8448-8455 VBN denotes studied
T6993 8460-8468 VBN denotes required
T6994 8456-8459 VBP denotes are
T6995 8469-8471 TO denotes to
T6996 8472-8479 VB denotes clarify
T6997 8480-8484 DT denotes this
T6998 8485-8496 NN denotes possibility
T6999 8496-8497 . denotes .
T7000 8497-8498 sentence denotes
T7017 8979-8981 , denotes ,
T7018 8981-8983 PRP denotes we
T7019 8993-8994 NN denotes β
T7020 8999-9003 NN denotes ESG1
T7021 8994-8995 HYPH denotes -
T7022 8995-8998 NN denotes geo
T7023 8998-8999 HYPH denotes -
T7024 9015-9021 NNS denotes clones
T7025 9003-9004 SYM denotes +
T7026 9004-9005 HYPH denotes /
T7027 9005-9006 SYM denotes -
T7028 9007-9009 NN denotes ES
T7029 9010-9014 NN denotes cell
T7030 9022-9026 IN denotes into
T7031 9027-9030 DT denotes the
T7032 9031-9042 NNS denotes blastocysts
T7033 9043-9045 IN denotes of
T7034 9046-9052 NN denotes C57BL6
T7035 9053-9057 NNS denotes mice
T7036 9057-9058 . denotes .
T7037 9058-9111 sentence denotes We obtained germline transmission from three clones.
T7038 9059-9061 PRP denotes We
T7039 9062-9070 VBD denotes obtained
T7040 9071-9079 NN denotes germline
T7041 9080-9092 NN denotes transmission
T7042 9093-9097 IN denotes from
T7043 9098-9103 CD denotes three
T7044 9104-9110 NNS denotes clones
T7045 9110-9111 . denotes .
T7046 9111-9238 sentence denotes We obtained ESG1-/- mice at the Mendelian ratios (36 wild-type, 69 ESG1+/-, and 45 ESG1-/-) from intercrosses of ESG1+/- mice.
T7047 9112-9114 PRP denotes We
T7048 9115-9123 VBD denotes obtained
T7049 9124-9128 NN denotes ESG1
T7050 9132-9136 NNS denotes mice
T7051 9128-9129 SYM denotes -
T7052 9129-9130 HYPH denotes /
T7053 9130-9131 SYM denotes -
T7054 9137-9139 IN denotes at
T7055 9140-9143 DT denotes the
T7056 9154-9160 NNS denotes ratios
T7057 9144-9153 JJ denotes Mendelian
T7058 9161-9162 -LRB- denotes (
T7059 9162-9164 CD denotes 36
T7060 9170-9174 NN denotes type
T7061 9165-9169 JJ denotes wild
T7062 9169-9170 HYPH denotes -
T7063 9174-9176 , denotes ,
T7064 9176-9178 CD denotes 69
T7065 9179-9183 NN denotes ESG1
T7066 9183-9184 SYM denotes +
T7067 9184-9185 HYPH denotes /
T7068 9185-9186 SYM denotes -
T7069 9186-9188 , denotes ,
T7070 9188-9191 CC denotes and
T7071 9192-9194 CD denotes 45
T7072 9195-9199 NN denotes ESG1
T7073 9199-9200 SYM denotes -
T7074 9200-9201 HYPH denotes /
T7075 9201-9202 SYM denotes -
T7076 9202-9203 -RRB- denotes )
T7077 9204-9208 IN denotes from
T7078 9209-9221 NNS denotes intercrosses
T7079 9222-9224 IN denotes of
T7080 9225-9229 NN denotes ESG1
T7081 9233-9237 NNS denotes mice
T7082 9229-9230 SYM denotes +
T7083 9230-9231 HYPH denotes /
T7084 9231-9232 SYM denotes -
T7085 9237-9238 . denotes .
T7086 9238-9327 sentence denotes These animals exhibited normal development, gross appearance, and fertility (not shown).
T7087 9239-9244 DT denotes These
T7088 9245-9252 NNS denotes animals
T7089 9253-9262 VBD denotes exhibited
T7090 9263-9269 JJ denotes normal
T7091 9270-9281 NN denotes development
T7092 9281-9283 , denotes ,
T7093 9283-9288 JJ denotes gross
T7094 9289-9299 NN denotes appearance
T7095 9299-9301 , denotes ,
T7096 9301-9304 CC denotes and
T7097 9305-9314 NN denotes fertility
T7098 9315-9316 -LRB- denotes (
T7099 9320-9325 VBN denotes shown
T7100 9316-9319 RB denotes not
T7101 9325-9326 -RRB- denotes )
T7102 9326-9327 . denotes .
T7103 9327-9422 sentence denotes Histological examination of testis and ovary could not identify any abnormalities (not shown).
T7104 9328-9340 JJ denotes Histological
T7105 9341-9352 NN denotes examination
T7106 9383-9391 VB denotes identify
T7107 9353-9355 IN denotes of
T7108 9356-9362 NN denotes testis
T7109 9363-9366 CC denotes and
T7110 9367-9372 NN denotes ovary
T7111 9373-9378 MD denotes could
T7112 9379-9382 RB denotes not
T7113 9392-9395 DT denotes any
T7114 9396-9409 NNS denotes abnormalities
T7115 9410-9411 -LRB- denotes (
T7116 9415-9420 VBN denotes shown
T7117 9411-9414 RB denotes not
T7118 9420-9421 -RRB- denotes )
T7119 9421-9422 . denotes .
T7120 9422-9523 sentence denotes These data demonstrated that ESG1 is dispensable for both mouse development and germ cell formation.
T7121 9423-9428 DT denotes These
T7122 9429-9433 NNS denotes data
T7123 9434-9446 VBD denotes demonstrated
T7124 9447-9451 IN denotes that
T7125 9457-9459 VBZ denotes is
T7126 9452-9456 NN denotes ESG1
T7127 9460-9471 JJ denotes dispensable
T7128 9472-9475 IN denotes for
T7129 9476-9480 CC denotes both
T7130 9487-9498 NN denotes development
T7131 9481-9486 NN denotes mouse
T7132 9499-9502 CC denotes and
T7133 9503-9507 NN denotes germ
T7134 9508-9512 NN denotes cell
T7135 9513-9522 NN denotes formation
T7136 9522-9523 . denotes .
T7137 9523-9630 sentence denotes We also generated ES cells from blastocysts obtained by intercrosses of ESG1+/- males and ESG1-/- females.
T7138 9524-9526 PRP denotes We
T7139 9532-9541 VBD denotes generated
T7140 9527-9531 RB denotes also
T7141 9542-9544 NN denotes ES
T7142 9545-9550 NNS denotes cells
T7143 9551-9555 IN denotes from
T7144 9556-9567 NNS denotes blastocysts
T7145 9568-9576 VBN denotes obtained
T7146 9577-9579 IN denotes by
T7147 9580-9592 NNS denotes intercrosses
T7148 9593-9595 IN denotes of
T7149 9596-9600 NN denotes ESG1
T7150 9604-9609 NNS denotes males
T7151 9600-9601 SYM denotes +
T7152 9601-9602 HYPH denotes /
T7153 9602-9603 SYM denotes -
T7154 9610-9613 CC denotes and
T7155 9614-9618 NN denotes ESG1
T7156 9622-9629 NNS denotes females
T7157 9618-9619 SYM denotes -
T7158 9619-9620 HYPH denotes /
T7159 9620-9621 SYM denotes -
T7160 9629-9630 . denotes .
T7161 9630-9695 sentence denotes Of the eight ES cell lines established, two clones were ESG1-/-.
T7162 9631-9633 IN denotes Of
T7163 9682-9686 VBD denotes were
T7164 9634-9637 DT denotes the
T7165 9652-9657 NNS denotes lines
T7166 9638-9643 CD denotes eight
T7167 9644-9646 NN denotes ES
T7168 9647-9651 NN denotes cell
T7169 9658-9669 VBN denotes established
T7170 9669-9671 , denotes ,
T7171 9671-9674 CD denotes two
T7172 9675-9681 NNS denotes clones
T7173 9687-9691 NN denotes ESG1
T7174 9691-9692 SYM denotes -
T7175 9692-9693 HYPH denotes /
T7176 9693-9694 SYM denotes -
T7177 9694-9695 . denotes .
T7178 9695-9849 sentence denotes These ESG1-null ES cells demonstrated normal morphology, proliferation, and differentiation (not shown), confirming that ESG1 is dispensable in ES cells.
T7179 9696-9701 DT denotes These
T7180 9715-9720 NNS denotes cells
T7181 9702-9706 NN denotes ESG1
T7182 9707-9711 JJ denotes null
T7183 9706-9707 HYPH denotes -
T7184 9712-9714 NN denotes ES
T7185 9721-9733 VBD denotes demonstrated
T7186 9734-9740 JJ denotes normal
T7187 9741-9751 NN denotes morphology
T7188 9751-9753 , denotes ,
T7189 9753-9766 NN denotes proliferation
T7190 9766-9768 , denotes ,
T7191 9768-9771 CC denotes and
T7192 9772-9787 NN denotes differentiation
T7193 9788-9789 -LRB- denotes (
T7194 9793-9798 VBN denotes shown
T7195 9789-9792 RB denotes not
T7196 9798-9799 -RRB- denotes )
T7197 9799-9801 , denotes ,
T7198 9801-9811 VBG denotes confirming
T7199 9812-9816 IN denotes that
T7200 9822-9824 VBZ denotes is
T7201 9817-9821 NN denotes ESG1
T7202 9825-9836 JJ denotes dispensable
T7203 9837-9839 IN denotes in
T7204 9840-9842 NN denotes ES
T7205 9843-9848 NNS denotes cells
T7206 9848-9849 . denotes .
T12055 883-898 NNS denotes ESG1pseudogenes
T12056 899-909 VBN denotes identified
T12057 910-912 IN denotes by
T12058 913-914 DT denotes a
T12059 921-927 NN denotes search
T12060 915-920 NNP denotes Blast
T12061 928-930 IN denotes of
T12062 931-936 NN denotes mouse
T12063 945-954 NNS denotes databases
T12064 937-944 JJ denotes genomic
T12065 954-955 . denotes .
T12066 955-1008 sentence denotes Substitution mutations are indicated by black lines.
T12067 956-968 NN denotes Substitution
T12068 969-978 NNS denotes mutations
T12069 983-992 VBN denotes indicated
T12070 979-982 VBP denotes are
T12071 993-995 IN denotes by
T12072 996-1001 JJ denotes black
T12073 1002-1007 NNS denotes lines
T12074 1007-1008 . denotes .
T12075 1008-1064 sentence denotes Intron-like gap sequences are indicated with triangles.
T12076 1009-1015 NN denotes Intron
T12077 1016-1020 JJ denotes like
T12078 1015-1016 HYPH denotes -
T12079 1025-1034 NNS denotes sequences
T12080 1021-1024 NN denotes gap
T12081 1039-1048 VBN denotes indicated
T12082 1035-1038 VBP denotes are
T12083 1049-1053 IN denotes with
T12084 1054-1063 NNS denotes triangles
T12085 1063-1064 . denotes .
T12086 1064-1114 sentence denotes Chromosomal localizations are shown on the right.
T12087 1065-1076 JJ denotes Chromosomal
T12088 1077-1090 NNS denotes localizations
T12089 1095-1100 VBN denotes shown
T12090 1091-1094 VBP denotes are
T12091 1101-1103 IN denotes on
T12092 1104-1107 DT denotes the
T12093 1108-1113 NN denotes right
T12094 1113-1114 . denotes .
T12275 2560-2563 NN denotes BAC
T12276 2564-2570 NNS denotes clones
T12277 2571-2581 VBG denotes containing
T12278 2582-2585 DT denotes the
T12279 2591-2595 NN denotes gene
T12280 2586-2590 NN denotes ESG1
T12281 2596-2599 CC denotes and
T12282 2600-2601 DT denotes a
T12283 2614-2624 NN denotes pseudogene
T12284 2602-2613 NN denotes duplication
T12285 2625-2626 -LRB- denotes (
T12286 2626-2628 NN denotes PS
T12287 2628-2629 -RRB- denotes )
T12288 2629-2630 . denotes .
T12289 2630-2682 sentence denotes A) Localization of the gene and PS on chromosome 9.
T12290 2631-2632 LS denotes A
T12291 2634-2646 NN denotes Localization
T12292 2632-2633 -RRB- denotes )
T12293 2647-2649 IN denotes of
T12294 2650-2653 DT denotes the
T12295 2654-2658 NN denotes gene
T12296 2659-2662 CC denotes and
T12297 2663-2665 NN denotes PS
T12298 2666-2668 IN denotes on
T12299 2669-2679 NN denotes chromosome
T12300 2680-2681 CD denotes 9
T12301 2681-2682 . denotes .
T12302 2682-2848 sentence denotes B) Sequence comparison of the gene and PS revealed that these are homologous from eight base pairs upstream of the first exon to 675 bp downstream of the third exon.
T12303 2683-2684 LS denotes B
T12304 2725-2733 VBD denotes revealed
T12305 2684-2685 -RRB- denotes )
T12306 2686-2694 NN denotes Sequence
T12307 2695-2705 NN denotes comparison
T12308 2706-2708 IN denotes of
T12309 2709-2712 DT denotes the
T12310 2713-2717 NN denotes gene
T12311 2718-2721 CC denotes and
T12312 2722-2724 NN denotes PS
T12313 2734-2738 IN denotes that
T12314 2745-2748 VBP denotes are
T12315 2739-2744 DT denotes these
T12316 2749-2759 JJ denotes homologous
T12317 2760-2764 IN denotes from
T12318 2765-2770 CD denotes eight
T12319 2776-2781 NNS denotes pairs
T12320 2771-2775 NN denotes base
T12321 2782-2790 RB denotes upstream
T12322 2791-2793 IN denotes of
T12323 2794-2797 DT denotes the
T12324 2804-2808 NN denotes exon
T12325 2798-2803 JJ denotes first
T12326 2809-2811 IN denotes to
T12327 2812-2815 CD denotes 675
T12328 2816-2818 NN denotes bp
T12329 2819-2829 RB denotes downstream
T12330 2830-2832 IN denotes of
T12331 2833-2836 DT denotes the
T12332 2843-2847 NN denotes exon
T12333 2837-2842 JJ denotes third
T12334 2847-2848 . denotes .
T12335 2848-2901 sentence denotes Substitution mutations are indicated by black lines.
T12336 2849-2861 NN denotes Substitution
T12337 2862-2871 NNS denotes mutations
T12338 2876-2885 VBN denotes indicated
T12339 2872-2875 VBP denotes are
T12340 2886-2888 IN denotes by
T12341 2889-2894 JJ denotes black
T12342 2895-2900 NNS denotes lines
T12343 2900-2901 . denotes .
T12344 2901-2949 sentence denotes The insertion is indicated by an open triangle.
T12345 2902-2905 DT denotes The
T12346 2906-2915 NN denotes insertion
T12347 2919-2928 VBN denotes indicated
T12348 2916-2918 VBZ denotes is
T12349 2929-2931 IN denotes by
T12350 2932-2934 DT denotes an
T12351 2940-2948 NN denotes triangle
T12352 2935-2939 JJ denotes open
T12353 2948-2949 . denotes .
T12543 2960-2968 NN denotes Promoter
T12544 2969-2977 NN denotes enhancer
T12545 2968-2969 HYPH denotes /
T12546 2978-2986 NN denotes activity
T12547 2987-2989 IN denotes of
T12548 2990-2993 DT denotes the
T12549 2999-3003 NN denotes gene
T12550 2994-2998 NN denotes ESG1
T12551 3004-3007 CC denotes and
T12552 3008-3018 NN denotes pseudogene
T12553 3018-3019 . denotes .
T12554 3019-3152 sentence denotes DNA fragments of ~6 kbp isolated from the 5' flanking regions of the gene and PS were transferred into luciferase reporter plasmids.
T12555 3020-3023 NN denotes DNA
T12556 3024-3033 NNS denotes fragments
T12557 3106-3117 VBN denotes transferred
T12558 3034-3036 IN denotes of
T12559 3037-3038 SYM denotes ~
T12560 3038-3039 CD denotes 6
T12561 3040-3043 NN denotes kbp
T12562 3044-3052 VBN denotes isolated
T12563 3053-3057 IN denotes from
T12564 3058-3061 DT denotes the
T12565 3074-3081 NNS denotes regions
T12566 3062-3063 CD denotes 5
T12567 3063-3064 SYM denotes '
T12568 3065-3073 NN denotes flanking
T12569 3082-3084 IN denotes of
T12570 3085-3088 DT denotes the
T12571 3089-3093 NN denotes gene
T12572 3094-3097 CC denotes and
T12573 3098-3100 NN denotes PS
T12574 3101-3105 VBD denotes were
T12575 3118-3122 IN denotes into
T12576 3123-3133 NN denotes luciferase
T12577 3134-3142 NN denotes reporter
T12578 3143-3151 NNS denotes plasmids
T12579 3151-3152 . denotes .
T12580 3152-3314 sentence denotes We introduced the reporter genes into undifferentiated ES cells (open columns), retinoic acid-treated ES cells (grey columns), and NIH3T3 cells (closed columns).
T12581 3153-3155 PRP denotes We
T12582 3156-3166 VBD denotes introduced
T12583 3167-3170 DT denotes the
T12584 3180-3185 NNS denotes genes
T12585 3171-3179 NN denotes reporter
T12586 3186-3190 IN denotes into
T12587 3191-3207 JJ denotes undifferentiated
T12588 3211-3216 NNS denotes cells
T12589 3208-3210 NN denotes ES
T12590 3217-3218 -LRB- denotes (
T12591 3223-3230 NNS denotes columns
T12592 3218-3222 JJ denotes open
T12593 3230-3231 -RRB- denotes )
T12594 3231-3233 , denotes ,
T12595 3233-3241 JJ denotes retinoic
T12596 3242-3246 NN denotes acid
T12597 3247-3254 VBN denotes treated
T12598 3246-3247 HYPH denotes -
T12599 3258-3263 NNS denotes cells
T12600 3255-3257 NN denotes ES
T12601 3264-3265 -LRB- denotes (
T12602 3270-3277 NNS denotes columns
T12603 3265-3269 JJ denotes grey
T12604 3277-3278 -RRB- denotes )
T12605 3278-3280 , denotes ,
T12606 3280-3283 CC denotes and
T12607 3284-3290 NN denotes NIH3T3
T12608 3291-3296 NNS denotes cells
T12609 3297-3298 -LRB- denotes (
T12610 3305-3312 NNS denotes columns
T12611 3298-3304 VBN denotes closed
T12612 3312-3313 -RRB- denotes )
T12613 3313-3314 . denotes .
T12614 3314-3400 sentence denotes Data represent the averages and standard deviations of three independent experiments.
T12615 3315-3319 NNS denotes Data
T12616 3320-3329 VBP denotes represent
T12617 3330-3333 DT denotes the
T12618 3334-3342 NNS denotes averages
T12619 3343-3346 CC denotes and
T12620 3347-3355 JJ denotes standard
T12621 3356-3366 NNS denotes deviations
T12622 3367-3369 IN denotes of
T12623 3370-3375 CD denotes three
T12624 3388-3399 NNS denotes experiments
T12625 3376-3387 JJ denotes independent
T12626 3399-3400 . denotes .
T13078 5183-5191 VBN denotes Targeted
T13079 5192-5202 NN denotes disruption
T13080 5203-5205 IN denotes of
T13081 5206-5209 DT denotes the
T13082 5221-5225 NN denotes gene
T13083 5210-5215 NN denotes mouse
T13084 5216-5220 NN denotes ESG1
T13085 5225-5226 . denotes .
T13086 5226-5249 sentence denotes A) Targeting strategy.
T13087 5227-5228 LS denotes A
T13088 5240-5248 NN denotes strategy
T13089 5228-5229 -RRB- denotes )
T13090 5230-5239 NN denotes Targeting
T13091 5248-5249 . denotes .
T13092 5249-5298 sentence denotes Homologous regions are indicated by thick lines.
T13093 5250-5260 JJ denotes Homologous
T13094 5261-5268 NNS denotes regions
T13095 5273-5282 VBN denotes indicated
T13096 5269-5272 VBP denotes are
T13097 5283-5285 IN denotes by
T13098 5286-5291 JJ denotes thick
T13099 5292-5297 NNS denotes lines
T13100 5297-5298 . denotes .
T13101 5298-5397 sentence denotes Recognition sites of PstI (P) and SpeI (S), which were used for Southern blot analyses, are shown.
T13102 5299-5310 NN denotes Recognition
T13103 5311-5316 NNS denotes sites
T13104 5391-5396 VBN denotes shown
T13105 5317-5319 IN denotes of
T13106 5320-5324 NN denotes PstI
T13107 5325-5326 -LRB- denotes (
T13108 5326-5327 NN denotes P
T13109 5327-5328 -RRB- denotes )
T13110 5329-5332 CC denotes and
T13111 5333-5337 NN denotes SpeI
T13112 5338-5339 -LRB- denotes (
T13113 5339-5340 NN denotes S
T13114 5340-5341 -RRB- denotes )
T13115 5341-5343 , denotes ,
T13116 5343-5348 WDT denotes which
T13117 5354-5358 VBN denotes used
T13118 5349-5353 VBD denotes were
T13119 5359-5362 IN denotes for
T13120 5363-5371 NNP denotes Southern
T13121 5372-5376 NN denotes blot
T13122 5377-5385 NNS denotes analyses
T13123 5385-5387 , denotes ,
T13124 5387-5390 VBP denotes are
T13125 5396-5397 . denotes .
T13126 5397-5526 sentence denotes The gene encoding diphtheria toxin A (DTA) was inserted at the 3' end of the targeting vectors to facilitate negative selection.
T13127 5398-5401 DT denotes The
T13128 5433-5434 NN denotes A
T13129 5402-5406 NN denotes gene
T13130 5407-5415 VBG denotes encoding
T13131 5416-5426 NN denotes diphtheria
T13132 5427-5432 NN denotes toxin
T13133 5445-5453 VBN denotes inserted
T13134 5435-5436 -LRB- denotes (
T13135 5436-5439 NN denotes DTA
T13136 5439-5440 -RRB- denotes )
T13137 5441-5444 VBD denotes was
T13138 5454-5456 IN denotes at
T13139 5457-5460 DT denotes the
T13140 5464-5467 NN denotes end
T13141 5461-5462 CD denotes 3
T13142 5462-5463 SYM denotes '
T13143 5468-5470 IN denotes of
T13144 5471-5474 DT denotes the
T13145 5485-5492 NNS denotes vectors
T13146 5475-5484 NN denotes targeting
T13147 5493-5495 TO denotes to
T13148 5496-5506 VB denotes facilitate
T13149 5507-5515 JJ denotes negative
T13150 5516-5525 NN denotes selection
T13151 5525-5526 . denotes .
T13152 5526-5589 sentence denotes B) Southern blot analyses confirming homologous recombination.
T13153 5527-5528 LS denotes B
T13154 5544-5552 NNS denotes analyses
T13155 5528-5529 -RRB- denotes )
T13156 5530-5538 NNP denotes Southern
T13157 5539-5543 NN denotes blot
T13158 5553-5563 VBG denotes confirming
T13159 5564-5574 JJ denotes homologous
T13160 5575-5588 NN denotes recombination
T13161 5588-5589 . denotes .
T13162 5589-5683 sentence denotes WT, wild-type ES cells; β, β-geo +/- ES cells; H, HygR +/- ES cells; -/-, ESG1-null ES cells.
T13163 5590-5592 NN denotes WT
T13164 5592-5594 , denotes ,
T13165 5594-5598 JJ denotes wild
T13166 5599-5603 NN denotes type
T13167 5598-5599 HYPH denotes -
T13168 5607-5612 NNS denotes cells
T13169 5604-5606 NN denotes ES
T13170 5612-5613 : denotes ;
T13171 5614-5615 NN denotes β
T13172 5615-5617 , denotes ,
T13173 5617-5618 NN denotes β
T13174 5619-5622 NN denotes geo
T13175 5618-5619 HYPH denotes -
T13176 5630-5635 NNS denotes cells
T13177 5623-5624 SYM denotes +
T13178 5624-5625 HYPH denotes /
T13179 5625-5626 SYM denotes -
T13180 5627-5629 NN denotes ES
T13181 5635-5636 : denotes ;
T13182 5637-5638 NN denotes H
T13183 5638-5640 , denotes ,
T13184 5640-5644 NN denotes HygR
T13185 5652-5657 NNS denotes cells
T13186 5645-5646 SYM denotes +
T13187 5646-5647 HYPH denotes /
T13188 5647-5648 SYM denotes -
T13189 5649-5651 NN denotes ES
T13190 5657-5658 : denotes ;
T13191 5659-5660 SYM denotes -
T13192 5661-5662 SYM denotes -
T13193 5660-5661 HYPH denotes /
T13194 5662-5664 , denotes ,
T13195 5664-5668 NN denotes ESG1
T13196 5669-5673 JJ denotes null
T13197 5668-5669 HYPH denotes -
T13198 5677-5682 NNS denotes cells
T13199 5674-5676 NN denotes ES
T13200 5682-5683 . denotes .
T13201 5683-5715 sentence denotes Numbers indicate clone numbers.
T13202 5684-5691 NNS denotes Numbers
T13203 5692-5700 VBP denotes indicate
T13204 5701-5706 NN denotes clone
T13205 5707-5714 NNS denotes numbers
T13206 5714-5715 . denotes .
T13207 5715-5874 sentence denotes C) Northern blot (upper) and western blot (lower) analyses of wild-type ES cells (WT), ESG1-null ES cells (-/-, three clones) and heterozygous ES cells (+/-).
T13208 5716-5717 LS denotes C
T13209 5766-5774 NNS denotes analyses
T13210 5717-5718 -RRB- denotes )
T13211 5719-5727 NNP denotes Northern
T13212 5728-5732 NN denotes blot
T13213 5733-5734 -LRB- denotes (
T13214 5734-5739 JJ denotes upper
T13215 5739-5740 -RRB- denotes )
T13216 5741-5744 CC denotes and
T13217 5745-5752 NNP denotes western
T13218 5753-5757 NN denotes blot
T13219 5758-5759 -LRB- denotes (
T13220 5759-5764 JJ denotes lower
T13221 5764-5765 -RRB- denotes )
T13222 5775-5777 IN denotes of
T13223 5778-5782 JJ denotes wild
T13224 5783-5787 NN denotes type
T13225 5782-5783 HYPH denotes -
T13226 5791-5796 NNS denotes cells
T13227 5788-5790 NN denotes ES
T13228 5797-5798 -LRB- denotes (
T13229 5798-5800 NN denotes WT
T13230 5800-5801 -RRB- denotes )
T13231 5801-5803 , denotes ,
T13232 5803-5807 NN denotes ESG1
T13233 5808-5812 JJ denotes null
T13234 5807-5808 HYPH denotes -
T13235 5816-5821 NNS denotes cells
T13236 5813-5815 NN denotes ES
T13237 5822-5823 -LRB- denotes (
T13238 5834-5840 NNS denotes clones
T13239 5823-5824 SYM denotes -
T13240 5825-5826 SYM denotes -
T13241 5824-5825 HYPH denotes /
T13242 5826-5828 , denotes ,
T13243 5828-5833 CD denotes three
T13244 5840-5841 -RRB- denotes )
T13245 5842-5845 CC denotes and
T13246 5846-5858 JJ denotes heterozygous
T13247 5862-5867 NNS denotes cells
T13248 5859-5861 NN denotes ES
T13249 5868-5869 -LRB- denotes (
T13250 5871-5872 SYM denotes -
T13251 5869-5870 SYM denotes +
T13252 5870-5871 HYPH denotes /
T13253 5872-5873 -RRB- denotes )
T13254 5873-5874 . denotes .
T13255 5874-5932 sentence denotes Northern blot was performed as previously described [20].
T13256 5875-5883 NNP denotes Northern
T13257 5884-5888 NN denotes blot
T13258 5893-5902 VBN denotes performed
T13259 5889-5892 VBD denotes was
T13260 5903-5905 IN denotes as
T13261 5917-5926 VBN denotes described
T13262 5906-5916 RB denotes previously
T13263 5927-5928 -LRB- denotes [
T13264 5928-5930 CD denotes 20
T13265 5930-5931 -RRB- denotes ]
T13266 5931-5932 . denotes .
T13267 5932-6047 sentence denotes To confirm the loading of equal amounts of RNA, ethidium bromide staining of ribosomal RNA is also shown (middle).
T13268 5933-5935 TO denotes To
T13269 5936-5943 VB denotes confirm
T13270 6032-6037 VBN denotes shown
T13271 5944-5947 DT denotes the
T13272 5948-5955 NN denotes loading
T13273 5956-5958 IN denotes of
T13274 5959-5964 JJ denotes equal
T13275 5965-5972 NNS denotes amounts
T13276 5973-5975 IN denotes of
T13277 5976-5979 NN denotes RNA
T13278 5979-5981 , denotes ,
T13279 5981-5989 NN denotes ethidium
T13280 5990-5997 NN denotes bromide
T13281 5998-6006 NN denotes staining
T13282 6007-6009 IN denotes of
T13283 6010-6019 JJ denotes ribosomal
T13284 6020-6023 NN denotes RNA
T13285 6024-6026 VBZ denotes is
T13286 6027-6031 RB denotes also
T13287 6038-6039 -LRB- denotes (
T13288 6039-6045 JJ denotes middle
T13289 6045-6046 -RRB- denotes )
T13290 6046-6047 . denotes .
T13587 7101-7109 NNS denotes Analyses
T13588 7110-7112 IN denotes of
T13589 7113-7117 NN denotes ESG1
T13590 7118-7122 JJ denotes null
T13591 7117-7118 HYPH denotes -
T13592 7126-7131 NNS denotes cells
T13593 7123-7125 NN denotes ES
T13594 7131-7132 . denotes .
T13595 7132-7207 sentence denotes A) The morphology of ESG1-null ES cell colonies grown on STO feeder cells.
T13596 7133-7134 LS denotes A
T13597 7140-7150 NN denotes morphology
T13598 7134-7135 -RRB- denotes )
T13599 7136-7139 DT denotes The
T13600 7151-7153 IN denotes of
T13601 7154-7158 NN denotes ESG1
T13602 7159-7163 JJ denotes null
T13603 7158-7159 HYPH denotes -
T13604 7172-7180 NNS denotes colonies
T13605 7164-7166 NN denotes ES
T13606 7167-7171 NN denotes cell
T13607 7181-7186 VBN denotes grown
T13608 7187-7189 IN denotes on
T13609 7190-7193 NN denotes STO
T13610 7201-7206 NNS denotes cells
T13611 7194-7200 NN denotes feeder
T13612 7206-7207 . denotes .
T13613 7207-7291 sentence denotes B) Growth curve of wild-type (WT), ESG1-null (-/-) and heterozygous (+/-) ES cells.
T13614 7208-7209 LS denotes B
T13615 7218-7223 NN denotes curve
T13616 7209-7210 -RRB- denotes )
T13617 7211-7217 NN denotes Growth
T13618 7224-7226 IN denotes of
T13619 7227-7231 JJ denotes wild
T13620 7232-7236 NN denotes type
T13621 7231-7232 HYPH denotes -
T13622 7285-7290 NNS denotes cells
T13623 7237-7238 -LRB- denotes (
T13624 7238-7240 NN denotes WT
T13625 7240-7241 -RRB- denotes )
T13626 7241-7243 , denotes ,
T13627 7243-7247 NN denotes ESG1
T13628 7247-7248 HYPH denotes -
T13629 7248-7252 JJ denotes null
T13630 7253-7254 -LRB- denotes (
T13631 7254-7255 SYM denotes -
T13632 7256-7257 SYM denotes -
T13633 7255-7256 HYPH denotes /
T13634 7257-7258 -RRB- denotes )
T13635 7259-7262 CC denotes and
T13636 7263-7275 JJ denotes heterozygous
T13637 7276-7277 -LRB- denotes (
T13638 7277-7278 SYM denotes +
T13639 7279-7280 SYM denotes -
T13640 7278-7279 HYPH denotes /
T13641 7280-7281 -RRB- denotes )
T13642 7282-7284 NN denotes ES
T13643 7290-7291 . denotes .
T13644 7291-7353 sentence denotes Each clone (1 × 104 cells/well) was plated in 24-well plates.
T13645 7292-7296 DT denotes Each
T13646 7297-7302 NN denotes clone
T13647 7328-7334 VBN denotes plated
T13648 7303-7304 -LRB- denotes (
T13649 7312-7317 NNS denotes cells
T13650 7304-7305 CD denotes 1
T13651 7308-7311 CD denotes 104
T13652 7306-7307 SYM denotes ×
T13653 7317-7318 SYM denotes /
T13654 7318-7322 NN denotes well
T13655 7322-7323 -RRB- denotes )
T13656 7324-7327 VBD denotes was
T13657 7335-7337 IN denotes in
T13658 7338-7340 CD denotes 24
T13659 7341-7345 NN denotes well
T13660 7340-7341 HYPH denotes -
T13661 7346-7352 NNS denotes plates
T13662 7352-7353 . denotes .
T13663 7353-7426 sentence denotes Cell numbers were determined with a Coulter counter at 2, 4, and 6 days.
T13664 7354-7358 NN denotes Cell
T13665 7359-7366 NNS denotes numbers
T13666 7372-7382 VBN denotes determined
T13667 7367-7371 VBD denotes were
T13668 7383-7387 IN denotes with
T13669 7388-7389 DT denotes a
T13670 7398-7405 NN denotes counter
T13671 7390-7397 NNP denotes Coulter
T13672 7406-7408 IN denotes at
T13673 7409-7410 CD denotes 2
T13674 7421-7425 NNS denotes days
T13675 7410-7412 , denotes ,
T13676 7412-7413 CD denotes 4
T13677 7413-7415 , denotes ,
T13678 7415-7418 CC denotes and
T13679 7419-7420 CD denotes 6
T13680 7425-7426 . denotes .
T13681 7426-7527 sentence denotes Data of +/- and -/- cells are shown as averages and standard deviations of three independent clones.
T13682 7427-7431 NNS denotes Data
T13683 7457-7462 VBN denotes shown
T13684 7432-7434 IN denotes of
T13685 7435-7436 SYM denotes +
T13686 7437-7438 SYM denotes -
T13687 7436-7437 HYPH denotes /
T13688 7447-7452 NNS denotes cells
T13689 7439-7442 CC denotes and
T13690 7443-7444 SYM denotes -
T13691 7445-7446 SYM denotes -
T13692 7444-7445 HYPH denotes /
T13693 7453-7456 VBP denotes are
T13694 7463-7465 IN denotes as
T13695 7466-7474 NNS denotes averages
T13696 7475-7478 CC denotes and
T13697 7479-7487 JJ denotes standard
T13698 7488-7498 NNS denotes deviations
T13699 7499-7501 IN denotes of
T13700 7502-7507 CD denotes three
T13701 7520-7526 NNS denotes clones
T13702 7508-7519 JJ denotes independent
T13703 7526-7527 . denotes .
T13704 7527-7616 sentence denotes C) A section of teratoma derived from ESG1-null ES cells (hematoxylin & eosin staining).
T13705 7528-7529 LS denotes C
T13706 7533-7540 NN denotes section
T13707 7529-7530 -RRB- denotes )
T13708 7531-7532 DT denotes A
T13709 7541-7543 IN denotes of
T13710 7544-7552 NN denotes teratoma
T13711 7553-7560 VBN denotes derived
T13712 7561-7565 IN denotes from
T13713 7566-7570 NN denotes ESG1
T13714 7571-7575 JJ denotes null
T13715 7570-7571 HYPH denotes -
T13716 7579-7584 NNS denotes cells
T13717 7576-7578 NN denotes ES
T13718 7585-7586 -LRB- denotes (
T13719 7606-7614 NN denotes staining
T13720 7586-7597 NN denotes hematoxylin
T13721 7598-7599 CC denotes &
T13722 7600-7605 NN denotes eosin
T13723 7614-7615 -RRB- denotes )
T13724 7615-7616 . denotes .
T13961 8508-8512 NN denotes Gene
T13962 8513-8523 NN denotes expression
T13963 8524-8532 NNS denotes analyses
T13964 8533-8535 IN denotes of
T13965 8536-8540 NN denotes ESG1
T13966 8541-8545 JJ denotes null
T13967 8540-8541 HYPH denotes -
T13968 8549-8554 NNS denotes cells
T13969 8546-8548 NN denotes ES
T13970 8554-8555 . denotes .
T13971 8555-8583 sentence denotes A) DNA microarray analyses.
T13972 8556-8557 LS denotes A
T13973 8574-8582 NNS denotes analyses
T13974 8557-8558 -RRB- denotes )
T13975 8559-8562 NN denotes DNA
T13976 8563-8573 NN denotes microarray
T13977 8582-8583 . denotes .
T13978 8583-8685 sentence denotes Total RNA from wild-type ES cells and ESG1-null ES cells were labeled with Cy3 and Cy5, respectively.
T13979 8584-8589 JJ denotes Total
T13980 8590-8593 NN denotes RNA
T13981 8646-8653 VBN denotes labeled
T13982 8594-8598 IN denotes from
T13983 8599-8603 JJ denotes wild
T13984 8604-8608 NN denotes type
T13985 8603-8604 HYPH denotes -
T13986 8612-8617 NNS denotes cells
T13987 8609-8611 NN denotes ES
T13988 8618-8621 CC denotes and
T13989 8622-8626 NN denotes ESG1
T13990 8627-8631 JJ denotes null
T13991 8626-8627 HYPH denotes -
T13992 8635-8640 NNS denotes cells
T13993 8632-8634 NN denotes ES
T13994 8641-8645 VBD denotes were
T13995 8654-8658 IN denotes with
T13996 8659-8662 NN denotes Cy3
T13997 8663-8666 CC denotes and
T13998 8667-8670 NN denotes Cy5
T13999 8670-8672 , denotes ,
T14000 8672-8684 RB denotes respectively
T14001 8684-8685 . denotes .
T14002 8685-8753 sentence denotes Samples were hybridized to Agilent Mouse Developmental Microarrays.
T14003 8686-8693 NNS denotes Samples
T14004 8699-8709 VBN denotes hybridized
T14005 8694-8698 VBD denotes were
T14006 8710-8712 IN denotes to
T14007 8713-8720 NNP denotes Agilent
T14008 8741-8752 NNS denotes Microarrays
T14009 8721-8726 NN denotes Mouse
T14010 8727-8740 JJ denotes Developmental
T14011 8752-8753 . denotes .
T14012 8753-8803 sentence denotes The averages of two independent clones are shown.
T14013 8754-8757 DT denotes The
T14014 8758-8766 NNS denotes averages
T14015 8797-8802 VBN denotes shown
T14016 8767-8769 IN denotes of
T14017 8770-8773 CD denotes two
T14018 8786-8792 NNS denotes clones
T14019 8774-8785 JJ denotes independent
T14020 8793-8796 VBP denotes are
T14021 8802-8803 . denotes .
T14022 8803-8829 sentence denotes B) Western blot analyses.
T14023 8804-8805 LS denotes B
T14024 8820-8828 NNS denotes analyses
T14025 8805-8806 -RRB- denotes )
T14026 8807-8814 NNP denotes Western
T14027 8815-8819 NN denotes blot
T14028 8828-8829 . denotes .
T14029 8829-8948 sentence denotes Cell lysates from ESG1+/- and ESG1-/- cells were examined for expression of ESG1, Oct3/4 and CDK4 with immunoblotting.
T14030 8830-8834 NN denotes Cell
T14031 8835-8842 NNS denotes lysates
T14032 8879-8887 VBN denotes examined
T14033 8843-8847 IN denotes from
T14034 8848-8852 NN denotes ESG1
T14035 8868-8873 NNS denotes cells
T14036 8852-8853 SYM denotes +
T14037 8853-8854 HYPH denotes /
T14038 8854-8855 SYM denotes -
T14039 8856-8859 CC denotes and
T14040 8860-8864 NN denotes ESG1
T14041 8864-8865 SYM denotes -
T14042 8865-8866 HYPH denotes /
T14043 8866-8867 SYM denotes -
T14044 8874-8878 VBD denotes were
T14045 8888-8891 IN denotes for
T14046 8892-8902 NN denotes expression
T14047 8903-8905 IN denotes of
T14048 8906-8910 NN denotes ESG1
T14049 8910-8912 , denotes ,
T14050 8912-8916 NN denotes Oct3
T14051 8916-8917 HYPH denotes /
T14052 8917-8918 CD denotes 4
T14053 8919-8922 CC denotes and
T14054 8923-8927 NN denotes CDK4
T14055 8928-8932 IN denotes with
T14056 8933-8947 NN denotes immunoblotting
T14057 8947-8948 . denotes .
T4103 456-458 IN denotes in
T4104 459-462 NN denotes DNA
T4105 463-472 NNS denotes fragments
T4106 473-476 IN denotes for
T4107 512-520 VBD denotes remained
T4108 477-482 WDT denotes which
T4109 483-486 DT denotes the
T4110 499-511 NN denotes localization
T4111 487-498 JJ denotes chromosomal
T4112 521-529 JJ denotes unmapped
T4113 529-530 . denotes .
T4114 530-670 sentence denotes While these pseudodgenes have significant homology to ESG1 cDNA, they could not produce functional proteins, because of critical mutations.
T4115 531-536 IN denotes While
T4116 556-560 VBP denotes have
T4117 537-542 DT denotes these
T4118 543-555 NNS denotes pseudodgenes
T4119 611-618 VB denotes produce
T4120 561-572 JJ denotes significant
T4121 573-581 NN denotes homology
T4183 1161-1168 JJ denotes similar
T4184 1169-1171 IN denotes to
T4185 1172-1175 DT denotes the
T4186 1181-1185 NN denotes gene
T4386 2214-2215 . denotes .
T4387 2215-2400 sentence denotes The 5' flanking region of this DNA fragment exhibited strong promoter/enhancer activity by luciferase reporter assays in undifferentiated ES cells, but not in somatic cells (Figure 3).
T4388 2216-2219 DT denotes The
T4389 2232-2238 NN denotes region
T4390 2220-2221 CD denotes 5
T4391 2221-2222 SYM denotes '
T4392 2223-2231 NN denotes flanking
T4393 2260-2269 VBD denotes exhibited
T4394 2239-2241 IN denotes of
T4477 3521-3522 DT denotes a
T4478 3541-3549 NN denotes sequence
T4479 3523-3525 CD denotes 68
T4480 3526-3529 NN denotes kbp
T4481 3530-3540 JJ denotes intergenic
T4482 3549-3551 , denotes ,
T4483 3551-3555 VBD denotes were
T4484 3565-3567 IN denotes in
T4485 3568-3576 JJ denotes opposite
T4486 3577-3587 NNS denotes directions
T6502 4864-4866 NN denotes 4A
T6503 4857-4863 NN denotes Figure
T6504 4866-4867 -RRB- denotes )
T6505 4868-4878 VBN denotes introduced
T6506 4879-4883 IN denotes into
T6507 4884-4887 NN denotes RF8
T6508 4891-4896 NNS denotes cells
T6509 4888-4890 NN denotes ES
T6510 4897-4899 IN denotes by
T6511 4900-4915 NN denotes electroporation
T6512 4915-4916 . denotes .
T6513 4916-5077 sentence denotes We obtained eight ES cell clones with correct homologous recombination of the β-geo targeting vector, which was confirmed by Southern blot analysis (Figure 4B).
T6514 4917-4919 PRP denotes We
T6515 4920-4928 VBD denotes obtained
T6516 4929-4934 CD denotes eight
T6573 6060-6062 sentence denotes mo
T6574 6060-6061 VBG denotes m
T6575 6061-6062 JJ denotes o
T6576 6062-6069 sentence denotes zygous
T6607 6157-6161 NN denotes G418
T6608 6162-6171 JJ denotes resistant
T6609 6161-6162 HYPH denotes -
T6610 6181-6187 VBN denotes tested
T6729 6733-6738 IN denotes after
T6730 6739-6742 DT denotes the
T6731 6743-6750 NN denotes removal
T6732 6751-6753 IN denotes of
T6733 6754-6762 NN denotes leukemia
T6734 6774-6780 NN denotes factor
T6735 6763-6773 JJ denotes inhibitory
T6736 6781-6782 -LRB- denotes (
T6737 6789-6791 NN denotes 5B
T6798 7617-7621 sentence denotes We e
T6799 7620-7621 NNS denotes e
T6800 7617-7618 NN denotes W
T6801 7618-7619 JJ denotes e
T6802 7621-7623 sentence denotes xa
T6810 7638-7648 NN denotes expression
T6811 7658-7660 IN denotes of
T6812 7661-7665 NN denotes ESG1
T6813 7672-7677 NNS denotes cells
T6814 7665-7666 SYM denotes -
T7006 8955-8956 NN denotes e
T7007 8958-8961 sentence denotes te
T7008 8958-8959 CD denotes t
T7009 8959-8960 JJ denotes e
T7010 8961-8966 sentence denotes ESG1-
T7011 8961-8965 NN denotes ESG1
T7012 8965-8966 SYM denotes -
T7013 8966-9058 sentence denotes knockout mice, we injected β-geo-ESG1+/- ES cell clones into the blastocysts of C57BL6 mice.
T7014 8984-8992 VBD denotes injected
T7015 8966-8974 NN denotes knockout
T4095 399-401 IN denotes In
T4096 445-455 VBN denotes identified
T4097 402-410 NN denotes addition
T4098 410-412 , denotes ,
T4099 412-415 CD denotes two
T4100 428-439 NNS denotes pseudogenes
T4101 416-427 JJ denotes intron-less
T4102 440-444 VBD denotes were
T4173 1115-1221 sentence denotes On chromosome 9, we identified a DNA fragment similar to the ESG1 gene that included two putative introns.
T4174 1115-1117 IN denotes On
T4175 1135-1145 VBD denotes identified
T4176 1118-1128 NN denotes chromosome
T4177 1129-1130 CD denotes 9
T4178 1130-1132 , denotes ,
T4179 1132-1134 PRP denotes we
T4180 1146-1147 DT denotes a
T4181 1152-1160 NN denotes fragment
T4182 1148-1151 NN denotes DNA
T4370 2155-2157 NN denotes AG
T4371 2157-2159 , denotes ,
T4372 2159-2169 VBG denotes fulfilling
T4373 2170-2173 DT denotes the
T4374 2180-2184 NN denotes rule
T4375 2174-2176 NN denotes GT
T4376 2177-2179 NN denotes AG
T4377 2176-2177 HYPH denotes -
T4378 2185-2187 IN denotes of
T4379 2188-2192 NN denotes exon
T4380 2193-2199 NN denotes intron
T4381 2192-2193 HYPH denotes -
T4382 2200-2209 NNS denotes junctions
T4383 2210-2211 -LRB- denotes [
T4384 2211-2213 CD denotes 19
T4385 2213-2214 -RRB- denotes ]
T4449 2549-3488 sentence denotes Figure 2 BAC clones containing the ESG1 gene and a duplication pseudogene (PS). A) Localization of the gene and PS on chromosome 9. B) Sequence comparison of the gene and PS revealed that these are homologous from eight base pairs upstream of the first exon to 675 bp downstream of the third exon. Substitution mutations are indicated by black lines. The insertion is indicated by an open triangle. Figure 3 Promoter/enhancer activity of the ESG1 gene and pseudogene. DNA fragments of ~6 kbp isolated from the 5' flanking regions of the gene and PS were transferred into luciferase reporter plasmids. We introduced the reporter genes into undifferentiated ES cells (open columns), retinoic acid-treated ES cells (grey columns), and NIH3T3 cells (closed columns). Data represent the averages and standard deviations of three independent experiments. We also found that the two BAC clones contained another ESG1-like sequence (Figure 2A).
T4450 3401-3403 PRP denotes We
T4451 3409-3414 VBD denotes found
T4452 3404-3408 RB denotes also
T4453 3415-3419 IN denotes that
T4454 3439-3448 VBD denotes contained
T4455 3420-3423 DT denotes the
T4456 3432-3438 NNS denotes clones
T4457 3424-3427 CD denotes two
T4458 3428-3431 NN denotes BAC
T4459 3449-3456 DT denotes another
T4460 3467-3475 NN denotes sequence
T4461 3457-3461 NN denotes ESG1
T4462 3462-3466 JJ denotes like
T4463 3461-3462 HYPH denotes -
T4464 3476-3477 -LRB- denotes (
T4465 3484-3486 NN denotes 2A
T4466 3477-3483 NN denotes Figure
T4467 3486-3487 -RRB- denotes )
T4468 3487-3488 . denotes .
T4469 3488-3588 sentence denotes The two sequences, separated by a 68 kbp intergenic sequence, were oriented in opposite directions.
T4470 3489-3492 DT denotes The
T4471 3497-3506 NNS denotes sequences
T4472 3493-3496 CD denotes two
T4473 3556-3564 VBN denotes oriented
T4474 3506-3508 , denotes ,
T4475 3508-3517 VBN denotes separated
T4476 3518-3520 IN denotes by
T6492 4795-4805 NN denotes hygromycin
T6493 4806-4815 JJ denotes resistant
T6494 4821-4822 -LRB- denotes (
T6495 4822-4826 NN denotes HygR
T6496 4826-4827 -RRB- denotes )
T6497 4828-4833 VBG denotes using
T6498 4834-4837 CD denotes two
T6499 4848-4855 NNS denotes vectors
T6500 4838-4847 NN denotes targeting
T6501 4856-4857 -LRB- denotes (
T6571 6055-6056 WDT denotes i
T6572 6056-6057 NNP denotes n
T6577 6062-6068 JJ denotes zygous
T6578 6069-6071 sentence denotes mu
T6579 6069-6070 VBN denotes m
T6580 6070-6071 RB denotes u
T6581 6071-6076 sentence denotes tant
T6582 6071-6072 DT denotes t
T6583 6072-6074 NN denotes an
T6584 6074-6075 NNS denotes t
T6585 6076-6149 sentence denotes ES cells, we introduced the β-geo vector into HygR heterozygous ES cells.
T6586 6089-6099 VBD denotes introduced
T6587 6079-6084 NNS denotes cells
T6588 6076-6078 NN denotes ES
T6589 6084-6086 , denotes ,
T6590 6086-6088 PRP denotes we
T6591 6100-6103 DT denotes the
T6592 6110-6116 NN denotes vector
T6593 6104-6105 NN denotes β
T6594 6106-6109 NN denotes geo
T6595 6105-6106 HYPH denotes -
T6596 6117-6121 IN denotes into
T6597 6122-6126 NN denotes HygR
T6598 6127-6139 JJ denotes heterozygous
T6599 6143-6148 NNS denotes cells
T6600 6140-6142 NN denotes ES
T6601 6148-6149 . denotes .
T6602 6149-6226 sentence denotes Of 105 G418-resistant colonies tested, 49 were homozygous for ESG1 deletion.
T6603 6150-6152 IN denotes Of
T6604 6192-6196 VBD denotes were
T6605 6153-6156 CD denotes 105
T6606 6172-6180 NNS denotes colonies
T6716 6682-6683 -LRB- denotes (
T6717 6690-6692 NN denotes 5B
T6718 6683-6689 NN denotes Figure
T6719 6692-6693 -RRB- denotes )
T6720 6693-6694 . denotes .
T6721 6694-6832 sentence denotes ESG1-/- cells differentiated normally after the removal of leukemia inhibitory factor (Figure 5B) or retinoic acid treatment (not shown).
T6722 6695-6699 NN denotes ESG1
T6723 6703-6708 NNS denotes cells
T6724 6699-6700 SYM denotes -
T6725 6700-6701 HYPH denotes /
T6726 6701-6702 SYM denotes -
T6727 6709-6723 VBD denotes differentiated
T6728 6724-6732 RB denotes normally
T6803 7622-7623 NN denotes a
T6804 7621-7622 NN denotes x
T6805 7623-7749 sentence denotes mined the gene expression profiles of ESG1-/- ES cells using oligonucleotide-based DNA microarrays representing ~20,000 genes.
T6806 7623-7628 VBD denotes mined
T6807 7629-7632 DT denotes the
T6808 7649-7657 NNS denotes profiles
T6809 7633-7637 NN denotes gene
T6896 8020-8027 NN denotes protein
T7001 8949-8952 sentence denotes To
T7002 8949-8951 JJ denotes To
T7003 8952-8958 sentence denotes genera
T7004 8952-8955 NNP denotes gen
T7005 8956-8958 NNS denotes ra
T7016 8975-8979 NNS denotes mice
R734 T4020 T4021 amod Chromosomal,localization
R735 T4022 T4021 cc and,localization
R736 T4023 T4021 conj structures,localization
R737 T4024 T4021 prep of,localization
R738 T4025 T4026 compound mouse,gene
R739 T4026 T4024 pobj gene,of
R740 T4027 T4026 compound ESG1,gene
R741 T4028 T4026 cc and,gene
R742 T4029 T4026 conj psedogenes,gene
R743 T4031 T4032 aux To,determine
R744 T4032 T4033 advcl determine,performed
R745 T4034 T4035 det the,localizations
R746 T4035 T4032 dobj localizations,determine
R747 T4036 T4035 amod chromosomal,localizations
R748 T4037 T4035 prep of,localizations
R749 T4038 T4039 det the,gene
R750 T4039 T4037 pobj gene,of
R751 T4040 T4039 compound mouse,gene
R752 T4041 T4039 compound ESG1,gene
R753 T4042 T4039 cc and,gene
R754 T4043 T4039 conj pseudogenes,gene
R755 T4044 T4033 punct ", ",performed
R756 T4045 T4033 nsubj we,performed
R757 T4046 T4047 det a,analysis
R758 T4047 T4033 dobj analysis,performed
R759 T4048 T4047 compound Blast,analysis
R760 T4049 T4047 prep of,analysis
R761 T4050 T4051 det the,database
R762 T4051 T4049 pobj database,of
R763 T4052 T4051 nmod mouse,database
R764 T4053 T4051 amod genomic,database
R765 T4054 T4033 prep with,performed
R766 T4055 T4056 det the,sequence
R767 T4056 T4054 pobj sequence,with
R768 T4057 T4056 compound ESG1,sequence
R769 T4058 T4056 compound cDNA,sequence
R770 T4059 T4056 prep as,sequence
R771 T4060 T4061 det a,query
R772 T4061 T4059 pobj query,as
R773 T4062 T4033 punct .,performed
R774 T4064 T4065 nsubj We,identified
R775 T4066 T4067 amod several,pseudogenes
R776 T4067 T4065 dobj pseudogenes,identified
R777 T4068 T4067 amod putative,pseudogenes
R778 T4069 T4067 prep without,pseudogenes
R779 T4070 T4069 pobj introns,without
R780 T4071 T4065 prep on,identified
R781 T4072 T4073 nmod chromosomes,X
R782 T4073 T4071 pobj X,on
R783 T4074 T4073 nummod 1,X
R784 T4075 T4073 punct ", ",X
R785 T4076 T4073 nummod 5,X
R786 T4077 T4073 punct ", ",X
R787 T4078 T4073 nummod 11,X
R788 T4079 T4073 punct ", ",X
R789 T4080 T4073 nummod 12,X
R790 T4081 T4073 punct ", ",X
R791 T4082 T4073 nummod 14,X
R792 T4083 T4073 punct ", ",X
R793 T4084 T4073 nummod 16,X
R794 T4085 T4073 punct ", ",X
R795 T4086 T4073 nummod 17,X
R796 T4087 T4073 punct ", ",X
R797 T4088 T4073 cc and,X
R798 T4089 T4090 punct (,Figure
R799 T4090 T4065 parataxis Figure,identified
R800 T4091 T4090 nummod 1,Figure
R801 T4092 T4090 punct ),Figure
R802 T4093 T4065 punct .,identified
R803 T4095 T4096 prep In,identified
R804 T4097 T4095 pobj addition,In
R805 T4098 T4096 punct ", ",identified
R806 T4099 T4100 nummod two,pseudogenes
R807 T4100 T4096 nsubjpass pseudogenes,identified
R808 T4101 T4100 amod intron-less,pseudogenes
R809 T4102 T4096 auxpass were,identified
R810 T4103 T4096 prep in,identified
R811 T4104 T4105 compound DNA,fragments
R812 T4105 T4103 pobj fragments,in
R813 T4106 T4107 prep for,remained
R814 T4107 T4105 relcl remained,fragments
R815 T4108 T4106 pobj which,for
R816 T4109 T4110 det the,localization
R817 T4110 T4107 nsubj localization,remained
R818 T4111 T4110 amod chromosomal,localization
R819 T4112 T4107 acomp unmapped,remained
R820 T4113 T4096 punct .,identified
R821 T4115 T4116 mark While,have
R822 T4116 T4119 advcl have,produce
R823 T4117 T4118 det these,pseudodgenes
R824 T4118 T4116 nsubj pseudodgenes,have
R825 T4120 T4121 amod significant,homology
R826 T4121 T4116 dobj homology,have
R827 T4122 T4121 prep to,homology
R862 T4161 T4156 attr hallmark,is
R863 T4162 T4161 prep of,hallmark
R864 T4163 T4164 amod pluripotent,genes
R865 T4164 T4162 pobj genes,of
R866 T4165 T4166 npadvmod cell,specific
R867 T4166 T4164 amod specific,genes
R868 T4167 T4166 punct -,specific
R869 T4168 T4169 punct [,18
R870 T4169 T4161 parataxis 18,hallmark
R871 T4170 T4169 punct ],18
R872 T4171 T4156 punct .,is
R873 T4174 T4175 prep On,identified
R874 T4176 T4174 pobj chromosome,On
R875 T4177 T4176 nummod 9,chromosome
R876 T4178 T4175 punct ", ",identified
R877 T4179 T4175 nsubj we,identified
R878 T4180 T4181 det a,fragment
R879 T4181 T4175 dobj fragment,identified
R880 T4182 T4181 compound DNA,fragment
R881 T4183 T4181 amod similar,fragment
R882 T4184 T4183 prep to,similar
R883 T4185 T4186 det the,gene
R884 T4186 T4184 pobj gene,to
R885 T4187 T4186 compound ESG1,gene
R886 T4188 T4189 dep that,included
R887 T4189 T4186 relcl included,gene
R888 T4190 T4191 nummod two,introns
R889 T4191 T4189 dobj introns,included
R890 T4192 T4191 amod putative,introns
R891 T4193 T4175 punct .,identified
R892 T4195 T4196 det These,exons
R893 T4196 T4201 nsubj exons,contained
R894 T4197 T4196 amod putative,exons
R895 T4198 T4196 amod first,exons
R896 T4199 T4198 cc and,first
R897 T4200 T4198 conj second,first
R898 T4202 T4201 punct ", ",contained
R899 T4203 T4201 advmod however,contained
R900 T4204 T4201 punct ", ",contained
R901 T4205 T4206 punct (,4
R902 T4206 T4201 parataxis 4,contained
R903 T4207 T4206 punct ),4
R904 T4208 T4209 amod multiple,mutations
R905 T4209 T4201 dobj mutations,contained
R906 T4210 T4209 prep of,mutations
R907 T4211 T4212 det the,sequence
R908 T4212 T4210 pobj sequence,of
R909 T4213 T4212 compound ESG1,sequence
R910 T4214 T4212 compound cDNA,sequence
R911 T4215 T4201 punct .,contained
R912 T4217 T4218 det The,exon
R913 T4218 T4221 nsubj exon,was
R914 T4219 T4218 amod putative,exon
R915 T4220 T4218 amod third,exon
R916 T4222 T4221 acomp identical,was
R917 T4223 T4222 prep to,identical
R918 T4224 T4223 pobj that,to
R919 T4225 T4224 prep of,that
R920 T4226 T4227 det the,gene
R921 T4227 T4225 pobj gene,of
R922 T4228 T4229 advmod previously,reported
R923 T4229 T4227 amod reported,gene
R924 T4230 T4227 compound ESG1,gene
R925 T4231 T4221 punct .,was
R926 T4233 T4234 advmod Also,identified
R927 T4235 T4234 prep on,identified
R928 T4236 T4235 pobj chromosome,on
R929 T4237 T4236 nummod 9,chromosome
R930 T4238 T4234 punct ", ",identified
R931 T4239 T4234 nsubj we,identified
R932 T4240 T4241 det another,fragment
R933 T4241 T4234 dobj fragment,identified
R934 T4242 T4241 compound DNA,fragment
R935 T4243 T4244 dep that,was
R936 T4244 T4241 relcl was,fragment
R937 T4245 T4244 acomp similar,was
R938 T4246 T4245 punct ", ",similar
R939 T4247 T4245 cc but,similar
R940 T4248 T4247 neg not,but
R941 T4249 T4245 conj identical,similar
R942 T4250 T4249 punct ", ",identical
R943 T4251 T4249 conj to,identical
R944 T4252 T4253 det the,exon
R945 T4253 T4251 pobj exon,to
R946 T4254 T4253 amod third,exon
R947 T4255 T4253 prep of,exon
R948 T4256 T4257 det the,gene
R949 T4257 T4255 pobj gene,of
R950 T4258 T4257 compound ESG1,gene
R951 T4259 T4234 punct .,identified
R952 T4261 T4262 det These,findings
R953 T4262 T4263 nsubj findings,suggest
R954 T4264 T4265 mark that,assembled
R955 T4265 T4263 ccomp assembled,suggest
R956 T4266 T4267 det these,sequences
R957 T4267 T4265 nsubjpass sequences,assembled
R958 T4268 T4269 npadvmod ESG1,like
R959 T4269 T4267 amod like,sequences
R960 T4270 T4269 punct -,like
R961 T4271 T4267 prep on,sequences
R962 T4272 T4271 pobj chromosome,on
R963 T4273 T4272 nummod 9,chromosome
R964 T4274 T4265 aux have,assembled
R965 T4275 T4265 neg not,assembled
R966 T4276 T4265 auxpass been,assembled
R967 T4277 T4265 advmod correctly,assembled
R968 T4278 T4263 punct .,suggest
R969 T4280 T4281 aux To,obtain
R970 T4281 T4282 advcl obtain,screened
R971 T4283 T4284 compound DNA,fragments
R972 T4284 T4281 dobj fragments,obtain
R973 T4285 T4284 acl containing,fragments
R974 T4286 T4287 det the,gene
R975 T4287 T4285 dobj gene,containing
R976 T4288 T4287 compound ESG1,gene
R977 T4289 T4282 punct ", ",screened
R978 T4290 T4282 nsubj we,screened
R979 T4291 T4292 det the,pool
R980 T4292 T4282 dobj pool,screened
R981 T4293 T4294 amod bacterial,chromosome
R982 T4294 T4292 nmod chromosome,pool
R983 T4295 T4294 amod artificial,chromosome
R984 T4296 T4294 punct (,chromosome
R985 T4297 T4294 appos BAC,chromosome
R986 T4298 T4292 punct ),pool
R987 T4299 T4292 compound DNA,pool
R988 T4300 T4282 prep by,screened
R989 T4301 T4300 pobj PCR,by
R990 T4302 T4282 advcl using,screened
R991 T4303 T4304 compound primer,pairs
R992 T4304 T4302 dobj pairs,using
R993 T4305 T4306 dep that,amplify
R994 T4306 T4304 relcl amplify,pairs
R995 T4307 T4306 aux would,amplify
R996 T4308 T4306 advmod only,amplify
R997 T4309 T4310 det the,gene
R998 T4310 T4306 dobj gene,amplify
R999 T4311 T4310 amod real,gene
R1000 T4312 T4306 punct ", ",amplify
R1001 T4313 T4314 neg not,pseudogenes
R1002 T4314 T4306 dep pseudogenes,amplify
R1003 T4315 T4314 det the,pseudogenes
R1004 T4316 T4282 punct .,screened
R1005 T4318 T4319 nsubj We,obtained
R1006 T4320 T4321 nummod two,clones
R1007 T4321 T4319 dobj clones,obtained
R1008 T4322 T4321 amod independent,clones
R1009 T4323 T4322 punct ", ",independent
R1010 T4324 T4322 cc but,independent
R1011 T4325 T4322 conj overlapping,independent
R1012 T4326 T4321 compound BAC,clones
R1013 T4327 T4319 punct .,obtained
R1014 T4329 T4330 compound Southern,blot
R1015 T4330 T4331 nsubj blot,demonstrated
R1016 T4332 T4330 appos analyses,blot
R1017 T4333 T4332 cc and,analyses
R1018 T4334 T4332 conj sequencing,analyses
R1019 T4335 T4336 mark that,contained
R1020 T4336 T4331 ccomp contained,demonstrated
R1021 T4337 T4338 det these,clones
R1022 T4338 T4336 nsubj clones,contained
R1023 T4339 T4340 det a,sequence
R1024 T4340 T4336 dobj sequence,contained
R1025 T4341 T4340 acl exhibiting,sequence
R1026 T4342 T4343 amod complete,identity
R1027 T4343 T4341 dobj identity,exhibiting
R1028 T4344 T4343 prep with,identity
R1029 T4345 T4346 compound ESG1,cDNA
R1030 T4346 T4344 pobj cDNA,with
R1031 T4347 T4348 dep that,interrupted
R1032 T4348 T4340 relcl interrupted,sequence
R1033 T4349 T4348 auxpass was,interrupted
R1034 T4350 T4348 agent by,interrupted
R1035 T4351 T4352 nummod two,introns
R1036 T4352 T4350 pobj introns,by
R1037 T4353 T4352 amod putative,introns
R1038 T4354 T4355 punct (,2A
R1039 T4355 T4348 parataxis 2A,interrupted
R1040 T4356 T4355 compound Figure,2A
R1041 T4357 T4355 punct ),2A
R1042 T4358 T4331 punct .,demonstrated
R1043 T4360 T4361 det The,sequences
R1044 T4361 T4364 nsubj sequences,begin
R1045 T4362 T4361 nummod two,sequences
R1046 T4363 T4361 compound intron,sequences
R1047 T4365 T4364 prep with,begin
R1048 T4366 T4365 pobj GT,with
R1049 T4367 T4364 cc and,begin
R1050 T4368 T4364 conj terminate,begin
R1051 T4369 T4368 prep with,terminate
R1052 T4370 T4369 pobj AG,with
R1053 T4371 T4364 punct ", ",begin
R1054 T4372 T4364 advcl fulfilling,begin
R1055 T4373 T4374 det the,rule
R1056 T4374 T4372 dobj rule,fulfilling
R1057 T4375 T4376 compound GT,AG
R1058 T4376 T4374 compound AG,rule
R1059 T4377 T4376 punct -,AG
R1060 T4378 T4374 prep of,rule
R1061 T4379 T4380 compound exon,intron
R1062 T4380 T4382 compound intron,junctions
R1063 T4381 T4380 punct -,intron
R1064 T4382 T4378 pobj junctions,of
R1065 T4383 T4384 punct [,19
R1066 T4384 T4372 parataxis 19,fulfilling
R1067 T4385 T4384 punct ],19
R1068 T4386 T4364 punct .,begin
R1069 T4388 T4389 det The,region
R1070 T4389 T4393 nsubj region,exhibited
R1071 T4390 T4389 nummod 5,region
R1072 T4391 T4390 punct ',5
R1073 T4392 T4389 compound flanking,region
R1074 T4394 T4389 prep of,region
R1075 T4395 T4396 det this,fragment
R1076 T4396 T4394 pobj fragment,of
R1077 T4397 T4396 compound DNA,fragment
R1078 T4398 T4399 amod strong,activity
R1079 T4399 T4393 dobj activity,exhibited
R1080 T4400 T4401 compound promoter,enhancer
R1081 T4401 T4399 compound enhancer,activity
R1082 T4402 T4401 punct /,enhancer
R1083 T4403 T4393 prep by,exhibited
R1084 T4404 T4405 compound luciferase,assays
R1085 T4405 T4403 pobj assays,by
R1086 T4406 T4405 compound reporter,assays
R1087 T4407 T4393 prep in,exhibited
R1088 T4408 T4409 amod undifferentiated,cells
R1089 T4409 T4407 pobj cells,in
R1090 T4410 T4409 compound ES,cells
R1091 T4411 T4407 punct ", ",in
R1092 T4412 T4407 cc but,in
R1093 T4413 T4412 neg not,but
R1094 T4414 T4407 conj in,in
R1095 T4415 T4416 amod somatic,cells
R1096 T4416 T4414 pobj cells,in
R1097 T4417 T4418 punct (,Figure
R1098 T4418 T4393 parataxis Figure,exhibited
R1099 T4419 T4418 nummod 3,Figure
R1100 T4420 T4418 punct ),Figure
R1101 T4421 T4393 punct .,exhibited
R1102 T4423 T4424 det The,fragment
R1103 T4424 T4426 nsubj fragment,showed
R1104 T4425 T4424 amod same,fragment
R1105 T4427 T4428 advmod much,weaker
R1106 T4428 T4429 amod weaker,activity
R1107 T4429 T4426 dobj activity,showed
R1108 T4430 T4426 prep after,showed
R1109 T4431 T4430 pobj induction,after
R1110 T4432 T4431 prep of,induction
R1111 T4433 T4432 pobj differentiation,of
R1112 T4434 T4431 prep by,induction
R1113 T4435 T4436 amod retinoic,acid
R1114 T4436 T4434 pobj acid,by
R1115 T4437 T4426 punct .,showed
R1116 T4439 T4440 nsubj We,concluded
R1117 T4441 T4442 mark that,is
R1125 T4515 T4513 advmod however,contained
R1126 T4516 T4513 punct ", ",contained
R1127 T4517 T4518 amod critical,substitutions
R1128 T4518 T4513 dobj substitutions,contained
R1129 T4519 T4518 compound nucleotide,substitutions
R1130 T4520 T4513 prep in,contained
R1131 T4521 T4520 pobj all,in
R1132 T4522 T4521 prep of,all
R1133 T4523 T4524 det the,exons
R1134 T4524 T4522 pobj exons,of
R1135 T4525 T4513 cc and,contained
R1136 T4526 T4527 nummod one,insertion
R1137 T4527 T4513 conj insertion,contained
R1138 T4528 T4527 compound nucleotide,insertion
R1139 T4529 T4527 prep in,insertion
R1140 T4530 T4529 pobj exon,in
R1142 T4531 T4530 nummod 2,exon
R1143 T4532 T4533 punct (,2B
R1146 T4533 T4527 parataxis 2B,insertion
R1147 T4534 T4533 compound Figure,2B
R1148 T4454 T4451 ccomp contained,found
R1149 T4535 T4533 punct ),2B
R1151 T4456 T4454 nsubj clones,contained
R1152 T4536 T4513 punct .,contained
R1155 T4459 T4460 det another,sequence
R1156 T4460 T4454 dobj sequence,contained
R1157 T4461 T4462 npadvmod ESG1,like
R1158 T4462 T4460 amod like,sequence
R1159 T4538 T4539 mark Although,conserved
R1160 T4463 T4462 punct -,like
R1161 T4464 T4465 punct (,2A
R1162 T4465 T4454 parataxis 2A,contained
R1163 T4539 T4550 advcl conserved,were
R1164 T4466 T4465 compound Figure,2A
R1165 T4467 T4465 punct ),2A
R1166 T4468 T4451 punct .,found
R1167 T4540 T4541 nummod 675,pairs
R1168 T4470 T4471 det The,sequences
R1169 T4471 T4473 nsubjpass sequences,oriented
R1170 T4541 T4539 nsubjpass pairs,conserved
R1171 T4472 T4471 nummod two,sequences
R1172 T4474 T4471 punct ", ",sequences
R1173 T4542 T4541 compound base,pairs
R1174 T4475 T4471 acl separated,sequences
R1175 T4476 T4475 agent by,separated
R1176 T4543 T4541 prep of,pairs
R1177 T4477 T4478 det a,sequence
R1178 T4478 T4476 pobj sequence,by
R1179 T4479 T4480 nummod 68,kbp
R1180 T4544 T4545 det the,regions
R1181 T4480 T4478 nmod kbp,sequence
R1182 T4481 T4478 amod intergenic,sequence
R1183 T4545 T4543 pobj regions,of
R1184 T4482 T4473 punct ", ",oriented
R1185 T4546 T4545 nummod 3,regions
R1186 T4483 T4473 auxpass were,oriented
R1187 T4484 T4473 prep in,oriented
R1188 T4485 T4486 amod opposite,directions
R1189 T4486 T4484 pobj directions,in
R1190 T4487 T4473 punct .,oriented
R1191 T4547 T4546 punct ',3
R1192 T4489 T4490 det The,sequence
R1193 T4490 T4494 nsubj sequence,exhibited
R1194 T4548 T4545 compound flanking,regions
R1195 T4491 T4492 npadvmod ESG1,like
R1196 T4492 T4490 amod like,sequence
R1197 T4493 T4492 punct -,like
R1198 T4549 T4539 auxpass were,conserved
R1199 T4495 T4496 amod greater,95
R1200 T4496 T4498 nummod 95,%
R1201 T4551 T4539 prep between,conserved
R1202 T4497 T4496 quantmod than,95
R1203 T4498 T4499 compound %,identity
R1204 T4499 T4494 dobj identity,exhibited
R1205 T4500 T4499 prep to,identity
R1206 T4552 T4553 det the,gene
R1207 T4501 T4502 det the,exons
R1208 T4502 T4500 pobj exons,to
R1209 T4503 T4502 cc and,exons
R1210 T4504 T4502 conj introns,exons
R1211 T4505 T4502 prep of,exons
R1212 T4553 T4551 pobj gene,between
R1213 T4506 T4507 det the,gene
R1214 T4507 T4505 pobj gene,of
R1215 T4508 T4507 compound ESG1,gene
R1216 T4554 T4553 compound ESG1,gene
R1217 T4509 T4494 punct .,exhibited
R1218 T4511 T4512 det This,sequence
R1219 T4555 T4553 cc and,gene
R1220 T4512 T4513 nsubj sequence,contained
R1221 T4556 T4557 det the,pseudogene
R1222 T4514 T4513 punct ", ",contained
R1223 T4557 T4553 conj pseudogene,gene
R1224 T4558 T4550 punct ", ",were
R1225 T4559 T4560 advmod only,pairs
R1226 T4621 T4622 det a,pseudogene
R1227 T4560 T4550 nsubj pairs,were
R1228 T4622 T4619 dobj pseudogene,represents
R1229 T4623 T4622 compound duplication,pseudogene
R1230 T4624 T4619 punct .,represents
R1231 T4561 T4560 nummod five,pairs
R1232 T4626 T4627 nsubj Bierbaum,reported
R1233 T4562 T4560 compound base,pairs
R1234 T4628 T4627 advmod previously,reported
R1235 T4629 T4630 det the,existence
R1236 T4563 T4560 prep of,pairs
R1237 T4630 T4627 dobj existence,reported
R1238 T4631 T4630 prep of,existence
R1239 T4632 T4633 nummod two,pseudogenes
R1240 T4564 T4565 det the,region
R1241 T4633 T4631 pobj pseudogenes,of
R1242 T4565 T4563 pobj region,of
R1243 T4634 T4633 prep with,pseudogenes
R1244 T4566 T4565 nummod 5,region
R1245 T4635 T4636 amod similar,organization
R1246 T4567 T4566 punct ',5
R1247 T4636 T4634 pobj organization,with
R1248 T4637 T4638 compound exon,intron
R1249 T4568 T4565 compound flanking,region
R1250 T4638 T4636 compound intron,organization
R1251 T4639 T4638 punct -,intron
R1252 T4640 T4627 prep as,reported
R1253 T4569 T4550 acomp identical,were
R1254 T4641 T4642 det the,gene
R1255 T4642 T4640 pobj gene,as
R1256 T4643 T4642 compound ESG1,gene
R1257 T4570 T4550 punct .,were
R1258 T4644 T4645 punct [,17
R1259 T4645 T4627 parataxis 17,reported
R1260 T4646 T4645 punct ],17
R1261 T4647 T4627 punct .,reported
R1262 T4572 T4573 det This,region
R1263 T4649 T4650 nsubj We,determine
R1264 T4651 T4650 aux could,determine
R1265 T4573 T4577 nsubj region,possess
R1266 T4652 T4650 neg not,determine
R1267 T4653 T4654 dep which,corresponds
R1268 T4654 T4650 ccomp corresponds,determine
R1269 T4574 T4573 nummod 5,region
R1270 T4655 T4653 prep of,which
R1271 T4656 T4657 det these,pseudogenes
R1272 T4575 T4574 punct ',5
R1273 T4657 T4655 pobj pseudogenes,of
R1274 T4658 T4657 nummod two,pseudogenes
R1275 T4576 T4573 compound flanking,region
R1276 T4659 T4654 prep to,corresponds
R1277 T4660 T4661 det the,one
R1278 T4661 T4659 pobj one,to
R1279 T4578 T4579 punct (,kbp
R1280 T4662 T4663 nsubj we,identified
R1281 T4663 T4661 advcl identified,one
R1282 T4664 T4661 cc or,one
R1283 T4665 T4666 det the,location
R1284 T4579 T4573 parataxis kbp,region
R1285 T4666 T4661 conj location,one
R1286 T4667 T4666 prep of,location
R1287 T4668 T4669 det the,pseudogene
R1288 T4580 T4581 punct ~,6
R1289 T4669 T4667 pobj pseudogene,of
R1290 T4670 T4669 amod remaining,pseudogene
R1291 T4671 T4650 punct .,determine
R1292 T4581 T4579 nummod 6,kbp
R1293 T4582 T4579 punct ),kbp
R1294 T4583 T4577 aux did,possess
R1295 T4584 T4577 neg not,possess
R1296 T4585 T4586 det any,activity
R1297 T4586 T4577 dobj activity,possess
R1298 T4587 T4588 compound promoter,enhancer
R1299 T4588 T4586 compound enhancer,activity
R1300 T4589 T4588 punct /,enhancer
R1301 T4590 T4577 prep in,possess
R1302 T4591 T4592 compound luciferase,reporter
R1303 T4592 T4593 compound reporter,assays
R1304 T4593 T4590 pobj assays,in
R1305 T4594 T4595 punct (,Figure
R1306 T4595 T4577 parataxis Figure,possess
R1307 T4596 T4595 nummod 3,Figure
R1308 T4597 T4595 punct ),Figure
R1309 T4598 T4577 punct .,possess
R1310 T4600 T4601 nsubj It,is
R1311 T4602 T4601 advmod thus,is
R1312 T4603 T4601 acomp unlikely,is
R1313 T4604 T4605 mark that,transcribed
R1314 T4605 T4601 ccomp transcribed,is
R1315 T4606 T4607 det this,sequence
R1316 T4607 T4605 nsubjpass sequence,transcribed
R1317 T4608 T4605 auxpass is,transcribed
R1318 T4609 T4605 cc or,transcribed
R1319 T4610 T4605 conj translated,transcribed
R1320 T4611 T4610 prep into,translated
R1321 T4612 T4613 det a,protein
R1322 T4613 T4611 pobj protein,into
R1323 T4614 T4613 amod functional,protein
R1324 T4615 T4601 punct .,is
R1325 T4617 T4618 det This,sequence
R1326 T4618 T4619 nsubj sequence,represents
R1327 T4620 T4619 advmod likely,represents
R1329 T6437 T6438 amod Targeted,disruption
R1330 T6439 T6438 prep of,disruption
R1331 T6440 T6441 det the,gene
R1332 T6441 T6439 pobj gene,of
R1333 T6442 T6441 compound mouse,gene
R1334 T6443 T6441 compound ESG1,gene
R1335 T6445 T6446 aux To,study
R1336 T6446 T6447 advcl study,deleted
R1337 T6448 T6449 det the,function
R1338 T6449 T6446 dobj function,study
R1339 T6450 T6449 prep of,function
R1340 T6451 T6450 pobj ESG1,of
R1341 T6452 T6447 punct ", ",deleted
R1342 T6453 T6447 nsubj we,deleted
R1343 T6454 T6455 det the,gene
R1344 T6455 T6447 dobj gene,deleted
R1345 T6456 T6447 prep by,deleted
R1346 T6457 T6458 amod homologous,recombination
R1347 T6458 T6456 pobj recombination,by
R1348 T6459 T6458 prep in,recombination
R1349 T6460 T6461 compound mouse,cells
R1350 T6461 T6459 pobj cells,in
R1351 T6462 T6461 compound ES,cells
R1352 T6463 T6447 punct .,deleted
R1353 T6465 T6466 nsubj We,replaced
R1354 T6467 T6468 det the,exons
R1355 T6468 T6466 dobj exons,replaced
R1356 T6469 T6468 nummod three,exons
R1357 T6470 T6466 prep with,replaced
R1358 T6471 T6472 preconj either,fusion
R1359 T6472 T6470 pobj fusion,with
R1360 T6473 T6472 det a,fusion
R1361 T6474 T6472 prep of,fusion
R1362 T6475 T6476 det the,genes
R1363 T6476 T6474 pobj genes,of
R1364 T6477 T6478 nmod neomycin,resistance
R1365 T6478 T6476 nmod resistance,genes
R1366 T6479 T6478 punct -,resistance
R1367 T6480 T6478 cc and,resistance
R1368 T6481 T6482 compound β,galactosidase
R1369 T6482 T6478 conj galactosidase,resistance
R1370 T6483 T6482 punct -,galactosidase
R1371 T6484 T6485 punct (,geo
R1372 T6485 T6476 parataxis geo,genes
R1373 T6486 T6485 compound β,geo
R1374 T6487 T6485 punct -,geo
R1386 T6499 T6497 dobj vectors,using
R1387 T6500 T6499 compound targeting,vectors
R1388 T6501 T6502 punct (,4A
R1389 T6502 T6499 parataxis 4A,vectors
R1390 T6503 T6502 compound Figure,4A
R1391 T6504 T6502 punct ),4A
R1392 T6505 T6499 acl introduced,vectors
R1393 T6506 T6505 prep into,introduced
R1394 T6507 T6508 compound RF8,cells
R1395 T6508 T6506 pobj cells,into
R1396 T6509 T6508 compound ES,cells
R1397 T6510 T6505 prep by,introduced
R1398 T6511 T6510 pobj electroporation,by
R1399 T6512 T6466 punct .,replaced
R1400 T6514 T6515 nsubj We,obtained
R1401 T6516 T6517 nummod eight,clones
R1402 T6517 T6515 dobj clones,obtained
R1403 T6518 T6517 compound ES,clones
R1404 T6519 T6517 compound cell,clones
R1405 T6520 T6515 prep with,obtained
R1406 T6521 T6522 amod correct,recombination
R1407 T6522 T6520 pobj recombination,with
R1408 T6523 T6522 amod homologous,recombination
R1409 T6524 T6522 prep of,recombination
R1410 T6525 T6526 det the,vector
R1411 T6526 T6524 pobj vector,of
R1412 T6527 T6528 compound β,geo
R1413 T6528 T6526 compound geo,vector
R1414 T6529 T6528 punct -,geo
R1415 T6530 T6526 compound targeting,vector
R1416 T6531 T6522 punct ", ",recombination
R1417 T6532 T6533 dep which,confirmed
R1418 T6533 T6522 relcl confirmed,recombination
R1419 T6534 T6533 auxpass was,confirmed
R1420 T6535 T6533 agent by,confirmed
R1421 T6536 T6537 compound Southern,blot
R1422 T6537 T6538 compound blot,analysis
R1423 T6538 T6535 pobj analysis,by
R1424 T6539 T6540 punct (,4B
R1425 T6540 T6533 parataxis 4B,confirmed
R1426 T6541 T6540 compound Figure,4B
R1427 T6542 T6540 punct ),4B
R1428 T6543 T6515 punct .,obtained
R1429 T6545 T6546 nsubj We,obtained
R1430 T6547 T6548 advmod only,clone
R1431 T6548 T6546 dobj clone,obtained
R1432 T6549 T6548 nummod one,clone
R1433 T6550 T6548 prep with,clone
R1434 T6551 T6552 amod correct,recombination
R1435 T6552 T6550 pobj recombination,with
R1436 T6553 T6552 amod homologous,recombination
R1437 T6554 T6552 prep of,recombination
R1438 T6555 T6556 det the,vector
R1439 T6556 T6554 pobj vector,of
R1440 T6557 T6556 compound HygR,vector
R1441 T6558 T6556 compound targeting,vector
R1442 T6559 T6546 punct .,obtained
R1443 T6562 T6563 amod T,o
R1444 T6564 T6563 prep o,o
R1469 T6613 T6604 acomp homozygous,were
R1470 T6614 T6613 prep for,homozygous
R1471 T6615 T6616 compound ESG1,deletion
R1472 T6616 T6614 pobj deletion,for
R1473 T6617 T6604 punct .,were
R1474 T6619 T6620 nmod Northern,blot
R1475 T6620 T6621 nmod blot,analyses
R1476 T6621 T6625 nsubj analyses,confirmed
R1477 T6622 T6620 cc and,blot
R1478 T6623 T6624 compound western,blot
R1479 T6624 T6620 conj blot,blot
R1480 T6626 T6627 det the,absence
R1481 T6627 T6625 dobj absence,confirmed
R1482 T6628 T6627 prep of,absence
R1483 T6629 T6628 pobj ESG1,of
R1484 T6630 T6627 prep in,absence
R1485 T6631 T6632 det these,cells
R1486 T6632 T6630 pobj cells,in
R1487 T6633 T6634 punct (,4C
R1488 T6634 T6625 parataxis 4C,confirmed
R1489 T6635 T6634 compound Figure,4C
R1490 T6636 T6634 punct ),4C
R1491 T6637 T6625 punct .,confirmed
R1492 T6639 T6640 prep In,replaced
R1493 T6641 T6642 nummod 29,clones
R1494 T6642 T6639 pobj clones,In
R1495 T6643 T6640 punct ", ",replaced
R1496 T6644 T6645 det the,vector
R1497 T6645 T6640 nsubj vector,replaced
R1498 T6646 T6647 compound β,geo
R1499 T6647 T6645 compound geo,vector
R1500 T6648 T6647 punct -,geo
R1501 T6649 T6640 aux had,replaced
R1502 T6650 T6651 det the,vector
R1503 T6651 T6640 dobj vector,replaced
R1504 T6652 T6651 compound HygR,vector
R1505 T6653 T6640 punct ", ",replaced
R1506 T6654 T6655 amod such,remained
R1507 T6655 T6640 advcl remained,replaced
R1508 T6656 T6655 mark that,remained
R1509 T6657 T6658 det the,cells
R1510 T6658 T6655 nsubj cells,remained
R1511 T6659 T6655 acomp heterozygous,remained
R1512 T6660 T6640 punct .,replaced
R1513 T6662 T6663 prep In,integrated
R1514 T6664 T6665 det the,clones
R1515 T6665 T6662 pobj clones,In
R1516 T6666 T6665 amod remaining,clones
R1517 T6667 T6665 nummod 27,clones
R1518 T6668 T6663 punct ", ",integrated
R1519 T6669 T6670 det the,vector
R1520 T6670 T6663 nsubjpass vector,integrated
R1521 T6671 T6672 compound β,geo
R1522 T6672 T6670 compound geo,vector
R1523 T6673 T6672 punct -,geo
R1524 T6674 T6663 auxpass was,integrated
R1525 T6675 T6663 prep at,integrated
R1526 T6676 T6677 amod non-homologous,sites
R1527 T6677 T6675 pobj sites,at
R1528 T6678 T6663 punct .,integrated
R1529 T6680 T6681 nmod ESG1,cells
R1530 T6681 T6686 nsubj cells,exhibited
R1531 T6682 T6680 punct -,ESG1
R1532 T6683 T6680 punct /,ESG1
R1533 T6684 T6680 punct -,ESG1
R1534 T6685 T6681 compound ES,cells
R1535 T6687 T6688 amod normal,morphology
R1536 T6688 T6686 dobj morphology,exhibited
R1537 T6689 T6690 punct (,5A
R1538 T6690 T6686 parataxis 5A,exhibited
R1539 T6691 T6690 compound Figure,5A
R1540 T6692 T6690 punct ),5A
R1541 T6693 T6686 punct .,exhibited
R1542 T6695 T6696 det These,cells
R1543 T6696 T6697 nsubj cells,proliferated
R1544 T6698 T6697 advmod also,proliferated
R1545 T6699 T6697 prep at,proliferated
R1546 T6700 T6701 det a,speed
R1547 T6701 T6699 pobj speed,at
R1548 T6702 T6701 amod comparable,speed
R1549 T6703 T6702 prep to,comparable
R1550 T6704 T6703 pobj that,to
R1551 T6705 T6704 prep of,that
R1552 T6706 T6707 det the,cells
R1553 T6707 T6705 pobj cells,of
R1554 T6708 T6707 nmod control,cells
R1555 T6709 T6710 punct (,heterozygous
R1556 T6710 T6708 parataxis heterozygous,control
R1557 T6711 T6710 cc and,heterozygous
R1558 T6712 T6713 amod wild,type
R1559 T6713 T6710 conj type,heterozygous
R1560 T6714 T6713 punct -,type
R1561 T6715 T6710 punct ),heterozygous
R1562 T6716 T6717 punct (,5B
R1563 T6717 T6697 parataxis 5B,proliferated
R1564 T6718 T6717 compound Figure,5B
R1565 T6719 T6717 punct ),5B
R1566 T6720 T6697 punct .,proliferated
R1567 T6722 T6723 nmod ESG1,cells
R1568 T6723 T6727 nsubj cells,differentiated
R1569 T6724 T6722 punct -,ESG1
R1570 T6725 T6722 punct /,ESG1
R1571 T6726 T6722 punct -,ESG1
R1572 T6728 T6727 advmod normally,differentiated
R1573 T6729 T6727 prep after,differentiated
R1574 T6730 T6731 det the,removal
R1575 T6731 T6729 pobj removal,after
R1576 T6732 T6731 prep of,removal
R1577 T6733 T6734 nmod leukemia,factor
R1578 T6734 T6732 pobj factor,of
R1579 T6735 T6734 amod inhibitory,factor
R1580 T6736 T6737 punct (,5B
R1581 T6737 T6734 parataxis 5B,factor
R1582 T6738 T6737 compound Figure,5B
R1583 T6739 T6737 punct ),5B
R1584 T6740 T6731 cc or,removal
R1614 T6772 T6773 punct (,5C
R1615 T6773 T6752 parataxis 5C,produced
R1616 T6774 T6773 compound Figure,5C
R1617 T6775 T6773 punct ),5C
R1618 T6776 T6752 punct .,produced
R1619 T6778 T6779 det These,results
R1620 T6779 T6780 nsubj results,indicate
R1621 T6781 T6782 mark that,is
R1622 T6782 T6780 ccomp is,indicate
R1623 T6783 T6782 nsubj ESG1,is
R1624 T6784 T6782 acomp dispensable,is
R1625 T6785 T6784 prep for,dispensable
R1626 T6786 T6787 det the,properties
R1627 T6787 T6785 pobj properties,for
R1628 T6788 T6789 compound self,renewal
R1629 T6789 T6787 compound renewal,properties
R1630 T6790 T6789 punct -,renewal
R1631 T6791 T6787 cc and,properties
R1632 T6792 T6787 conj pluripotency,properties
R1633 T6793 T6792 prep of,pluripotency
R1634 T6794 T6795 compound ES,cells
R1635 T6795 T6793 pobj cells,of
R1636 T6796 T6780 punct .,indicate
R1653 T6822 T6818 dobj microarrays,using
R1654 T6823 T6822 compound DNA,microarrays
R1655 T6824 T6822 acl representing,microarrays
R1656 T6825 T6826 punct ~,"20,000"
R1657 T6826 T6827 nummod "20,000",genes
R1658 T6827 T6824 dobj genes,representing
R1659 T6828 T6806 punct .,mined
R1660 T6830 T6831 prep In,identified
R1661 T6832 T6830 pobj comparison,In
R1662 T6833 T6832 prep to,comparison
R1663 T6834 T6835 compound control,cells
R1664 T6835 T6833 pobj cells,to
R1665 T6836 T6835 compound ES,cells
R1666 T6837 T6831 punct ", ",identified
R1667 T6838 T6831 nsubjpass ESG1,identified
R1668 T6839 T6831 auxpass was,identified
R1669 T6840 T6831 prep as,identified
R1670 T6841 T6842 det the,gene
R1671 T6842 T6840 pobj gene,as
R1672 T6843 T6842 acl reduced,gene
R1673 T6844 T6843 prep to,reduced
R1674 T6845 T6846 det the,extent
R1675 T6846 T6844 pobj extent,to
R1676 T6847 T6846 amod greatest,extent
R1677 T6848 T6843 prep in,reduced
R1678 T6849 T6850 nmod ESG1,cells
R1679 T6850 T6848 pobj cells,in
R1680 T6851 T6849 punct -,ESG1
R1681 T6852 T6849 punct /,ESG1
R1682 T6853 T6849 punct -,ESG1
R1683 T6854 T6850 compound ES,cells
R1684 T6855 T6856 punct (,6A
R1685 T6856 T6842 parataxis 6A,gene
R1686 T6857 T6856 compound Figure,6A
R1687 T6858 T6856 punct ),6A
R1688 T6859 T6831 punct .,identified
R1689 T6861 T6862 det The,expression
R1690 T6862 T6863 nsubj expression,was
R1691 T6864 T6862 prep of,expression
R1692 T6865 T6866 compound ES,cell
R1693 T6866 T6867 compound cell,genes
R1694 T6867 T6864 pobj genes,of
R1695 T6868 T6867 compound marker,genes
R1696 T6869 T6867 punct ", ",genes
R1697 T6870 T6871 amod such,as
R1698 T6871 T6867 prep as,genes
R1699 T6872 T6871 pobj Nanog,as
R1700 T6873 T6872 cc and,Nanog
R1701 T6874 T6872 conj Oct3,Nanog
R1702 T6875 T6874 punct /,Oct3
R1703 T6876 T6874 nummod 4,Oct3
R1704 T6877 T6863 punct ", ",was
R1705 T6878 T6863 acomp normal,was
R1706 T6879 T6863 prep in,was
R1707 T6880 T6881 nmod ESG1,cells
R1708 T6881 T6879 pobj cells,in
R1709 T6882 T6880 punct -,ESG1
R1710 T6883 T6880 punct /,ESG1
R1711 T6884 T6880 punct -,ESG1
R1712 T6885 T6881 compound ES,cells
R1713 T6886 T6863 punct .,was
R1714 T6888 T6889 nsubj We,confirmed
R1715 T6890 T6891 amod normal,expression
R1716 T6891 T6889 dobj expression,confirmed
R1717 T6892 T6891 nmod Oct3,expression
R1718 T6893 T6892 punct /,Oct3
R1719 T6894 T6892 nummod 4,Oct3
R1720 T6895 T6891 prep at,expression
R1721 T6896 T6897 compound protein,levels
R1722 T6897 T6895 pobj levels,at
R1723 T6898 T6889 prep by,confirmed
R1724 T6899 T6900 compound Western,blot
R1725 T6900 T6898 pobj blot,by
R1726 T6901 T6902 punct (,6B
R1727 T6902 T6889 parataxis 6B,confirmed
R1728 T6903 T6902 compound Figure,6B
R1729 T6904 T6902 punct ),6B
R1730 T6905 T6889 punct .,confirmed
R1731 T6907 T6908 det The,profiles
R1732 T6908 T6912 nsubj profiles,were
R1733 T6909 T6908 amod overall,profiles
R1734 T6910 T6911 compound gene,expression
R1735 T6911 T6908 compound expression,profiles
R1736 T6913 T6912 acomp similar,were
R1737 T6914 T6912 prep between,were
R1738 T6915 T6916 compound control,cells
R1739 T6916 T6914 pobj cells,between
R1740 T6917 T6916 compound ES,cells
R1741 T6918 T6916 cc and,cells
R1742 T6919 T6920 nmod ESG1,cells
R1743 T6920 T6916 conj cells,cells
R1744 T6921 T6919 punct -,ESG1
R1745 T6922 T6919 punct /,ESG1
R1746 T6923 T6919 punct -,ESG1
R1747 T6924 T6920 compound ES,cells
R1748 T6925 T6912 punct .,were
R1749 T6927 T6928 amod Several,genes
R1750 T6928 T6929 nsubj genes,exhibited
R1751 T6930 T6931 det a,reduction
R1752 T6931 T6929 dobj reduction,exhibited
R1753 T6932 T6933 amod greater,two-fold
R1754 T6933 T6931 nummod two-fold,reduction
R1755 T6934 T6933 quantmod than,two-fold
R1756 T6935 T6929 prep in,exhibited
R1757 T6936 T6937 nmod ESG1,cells
R1758 T6937 T6935 pobj cells,in
R1759 T6938 T6936 punct -,ESG1
R1760 T6939 T6936 punct /,ESG1
R1761 T6940 T6936 punct -,ESG1
R1762 T6941 T6929 punct ", ",exhibited
R1763 T6942 T6929 prep including,exhibited
R1764 T6943 T6942 pobj Krt1,including
R1765 T6944 T6943 punct -,Krt1
R1766 T6945 T6943 nummod 8,Krt1
R1767 T6946 T6943 punct ", ",Krt1
R1768 T6947 T6943 conj Pem,Krt1
R1769 T6948 T6947 punct ", ",Pem
R1770 T6949 T6947 conj Ctgf,Pem
R1771 T6950 T6949 punct ", ",Ctgf
R1772 T6951 T6949 conj Ptgs2,Ctgf
R1773 T6952 T6951 punct ", ",Ptgs2
R1774 T6953 T6951 conj Igf2,Ptgs2
R1775 T6954 T6953 cc and,Igf2
R1776 T6955 T6953 conj Inhba,Igf2
R1777 T6956 T6929 punct .,exhibited
R1778 T6958 T6959 det These,genes
R1779 T6959 T6960 nsubjpass genes,regulated
R1780 T6961 T6960 aux might,regulated
R1781 T6962 T6960 auxpass be,regulated
R1782 T6963 T6960 advmod directly,regulated
R1857 T7059 T7060 nummod 36,type
R1858 T7060 T7056 appos type,ratios
R1859 T7061 T7060 amod wild,type
R1860 T7062 T7060 punct -,type
R1861 T7063 T7060 punct ", ",type
R1862 T7064 T7065 nummod 69,ESG1
R1863 T7065 T7060 conj ESG1,type
R1864 T7066 T7065 punct +,ESG1
R1865 T7067 T7065 punct /,ESG1
R1866 T7068 T7065 punct -,ESG1
R1867 T7069 T7065 punct ", ",ESG1
R1868 T7070 T7065 cc and,ESG1
R1869 T7071 T7072 nummod 45,ESG1
R1870 T7072 T7065 conj ESG1,ESG1
R1871 T7073 T7072 punct -,ESG1
R1872 T7074 T7072 punct /,ESG1
R1873 T7075 T7072 punct -,ESG1
R1874 T7076 T7056 punct ),ratios
R1875 T7077 T7048 prep from,obtained
R1876 T7078 T7077 pobj intercrosses,from
R1877 T7079 T7078 prep of,intercrosses
R1878 T7080 T7081 nmod ESG1,mice
R1879 T7081 T7079 pobj mice,of
R1880 T7082 T7080 punct +,ESG1
R1881 T7083 T7080 punct /,ESG1
R1882 T7084 T7080 punct -,ESG1
R1883 T7085 T7048 punct .,obtained
R1884 T7087 T7088 det These,animals
R1885 T7088 T7089 nsubj animals,exhibited
R1886 T7090 T7091 amod normal,development
R1887 T7091 T7089 dobj development,exhibited
R1888 T7092 T7091 punct ", ",development
R1889 T7093 T7094 amod gross,appearance
R1890 T7094 T7091 conj appearance,development
R1891 T7095 T7094 punct ", ",appearance
R1892 T7096 T7094 cc and,appearance
R1893 T7097 T7094 conj fertility,appearance
R1894 T7098 T7099 punct (,shown
R1895 T7099 T7089 parataxis shown,exhibited
R1896 T7100 T7099 neg not,shown
R1897 T7121 T7122 det These,data
R1898 T7101 T7099 punct ),shown
R1899 T7122 T7123 nsubj data,demonstrated
R1900 T7102 T7089 punct .,exhibited
R1901 T7124 T7125 mark that,is
R1902 T7125 T7123 ccomp is,demonstrated
R1903 T7126 T7125 nsubj ESG1,is
R1904 T7127 T7125 acomp dispensable,is
R1905 T7128 T7127 prep for,dispensable
R1906 T7129 T7130 preconj both,development
R1907 T7130 T7128 pobj development,for
R1908 T7131 T7130 compound mouse,development
R1909 T7104 T7105 amod Histological,examination
R1910 T7132 T7130 cc and,development
R1911 T7133 T7134 compound germ,cell
R1912 T7134 T7135 compound cell,formation
R1913 T7135 T7130 conj formation,development
R1914 T7136 T7123 punct .,demonstrated
R1915 T7138 T7139 nsubj We,generated
R1916 T7105 T7106 nsubj examination,identify
R1917 T7140 T7139 advmod also,generated
R1918 T7141 T7142 compound ES,cells
R1919 T7142 T7139 dobj cells,generated
R1920 T7107 T7105 prep of,examination
R1921 T7143 T7139 prep from,generated
R1922 T7144 T7143 pobj blastocysts,from
R1923 T7145 T7144 acl obtained,blastocysts
R1924 T7146 T7145 prep by,obtained
R1925 T7108 T7107 pobj testis,of
R1926 T7147 T7146 pobj intercrosses,by
R1927 T7148 T7147 prep of,intercrosses
R1928 T7109 T7108 cc and,testis
R1929 T7149 T7150 nmod ESG1,males
R1930 T7110 T7108 conj ovary,testis
R1931 T7150 T7148 pobj males,of
R1932 T7151 T7149 punct +,ESG1
R1933 T7152 T7149 punct /,ESG1
R1934 T7153 T7149 punct -,ESG1
R1935 T7111 T7106 aux could,identify
R1936 T7154 T7150 cc and,males
R1937 T7155 T7156 nmod ESG1,females
R1938 T7112 T7106 neg not,identify
R1939 T7156 T7150 conj females,males
R1940 T7157 T7155 punct -,ESG1
R1941 T7158 T7155 punct /,ESG1
R1942 T7159 T7155 punct -,ESG1
R1943 T7113 T7114 det any,abnormalities
R1944 T7160 T7139 punct .,generated
R1945 T7162 T7163 prep Of,were
R1946 T7114 T7106 dobj abnormalities,identify
R1947 T7164 T7165 det the,lines
R1948 T7165 T7162 pobj lines,Of
R1949 T7166 T7165 nummod eight,lines
R1950 T7115 T7116 punct (,shown
R1951 T7167 T7165 compound ES,lines
R1952 T7168 T7165 compound cell,lines
R1953 T7169 T7165 acl established,lines
R1954 T7116 T7106 parataxis shown,identify
R1955 T7170 T7163 punct ", ",were
R1956 T7171 T7172 nummod two,clones
R1957 T7172 T7163 nsubj clones,were
R1958 T7173 T7163 attr ESG1,were
R1959 T7174 T7173 punct -,ESG1
R1960 T7175 T7173 punct /,ESG1
R1961 T7176 T7173 punct -,ESG1
R1962 T7177 T7163 punct .,were
R1963 T7117 T7116 neg not,shown
R1964 T7179 T7180 det These,cells
R1965 T7180 T7185 nsubj cells,demonstrated
R1966 T7181 T7182 npadvmod ESG1,null
R1967 T7118 T7116 punct ),shown
R1968 T7182 T7180 amod null,cells
R1969 T7183 T7182 punct -,null
R1970 T7184 T7180 compound ES,cells
R1971 T7119 T7106 punct .,identify
R1972 T7186 T7187 amod normal,morphology
R1973 T7187 T7185 dobj morphology,demonstrated
R1974 T7188 T7187 punct ", ",morphology
R1975 T7189 T7187 conj proliferation,morphology
R1976 T7190 T7189 punct ", ",proliferation
R1977 T7191 T7189 cc and,proliferation
R1978 T7192 T7189 conj differentiation,proliferation
R1979 T7193 T7194 punct (,shown
R1980 T7194 T7192 parataxis shown,differentiation
R1981 T7195 T7194 neg not,shown
R1982 T7196 T7194 punct ),shown
R1983 T7197 T7185 punct ", ",demonstrated
R1984 T7198 T7185 advcl confirming,demonstrated
R1985 T7199 T7200 mark that,is
R1986 T7200 T7198 ccomp is,confirming
R1987 T7201 T7200 nsubj ESG1,is
R1988 T7202 T7200 acomp dispensable,is
R1989 T7203 T7200 prep in,is
R1990 T7204 T7205 compound ES,cells
R1991 T7205 T7203 pobj cells,in
R1992 T7206 T7185 punct .,demonstrated
R3462 T12056 T12055 acl identified,ESG1pseudogenes
R3463 T12057 T12056 agent by,identified
R3464 T12058 T12059 det a,search
R3465 T12059 T12057 pobj search,by
R3466 T12060 T12059 compound Blast,search
R3467 T12061 T12059 prep of,search
R3468 T12062 T12063 nmod mouse,databases
R3469 T12063 T12061 pobj databases,of
R3470 T12064 T12063 amod genomic,databases
R3471 T12065 T12055 punct .,ESG1pseudogenes
R3472 T12067 T12068 compound Substitution,mutations
R3473 T12068 T12069 nsubjpass mutations,indicated
R3474 T12070 T12069 auxpass are,indicated
R3475 T12071 T12069 agent by,indicated
R3476 T12072 T12073 amod black,lines
R3477 T12073 T12071 pobj lines,by
R3478 T12074 T12069 punct .,indicated
R3479 T12076 T12077 npadvmod Intron,like
R3480 T12077 T12079 amod like,sequences
R3481 T12078 T12077 punct -,like
R3482 T12079 T12081 nsubjpass sequences,indicated
R3483 T12080 T12079 compound gap,sequences
R3484 T12082 T12081 auxpass are,indicated
R3485 T12083 T12081 prep with,indicated
R3486 T12084 T12083 pobj triangles,with
R3487 T12085 T12081 punct .,indicated
R3488 T12087 T12088 amod Chromosomal,localizations
R3489 T12088 T12089 nsubjpass localizations,shown
R3490 T12090 T12089 auxpass are,shown
R3491 T12091 T12089 prep on,shown
R3492 T12092 T12093 det the,right
R3493 T12093 T12091 pobj right,on
R3494 T12094 T12089 punct .,shown
R3495 T12275 T12276 compound BAC,clones
R3496 T12276 T12277 nsubj clones,containing
R3497 T12278 T12279 det the,gene
R3498 T12279 T12277 dobj gene,containing
R3499 T12280 T12279 compound ESG1,gene
R3500 T12281 T12279 cc and,gene
R3501 T12282 T12283 det a,pseudogene
R3502 T12283 T12279 conj pseudogene,gene
R3503 T12284 T12283 compound duplication,pseudogene
R3504 T12285 T12286 punct (,PS
R3505 T12286 T12283 parataxis PS,pseudogene
R3506 T12287 T12286 punct ),PS
R3507 T12288 T12277 punct .,containing
R3508 T12290 T12291 meta A,Localization
R3509 T12292 T12290 punct ),A
R3510 T12293 T12291 prep of,Localization
R3511 T12294 T12295 det the,gene
R3512 T12295 T12293 pobj gene,of
R3513 T12296 T12295 cc and,gene
R3514 T12297 T12295 conj PS,gene
R3515 T12298 T12291 prep on,Localization
R3516 T12299 T12298 pobj chromosome,on
R3517 T12300 T12299 nummod 9,chromosome
R3518 T12301 T12291 punct .,Localization
R3519 T12303 T12304 meta B,revealed
R3520 T12305 T12303 punct ),B
R3521 T12306 T12307 compound Sequence,comparison
R3522 T12307 T12304 nsubj comparison,revealed
R3523 T12308 T12307 prep of,comparison
R3524 T12309 T12310 det the,gene
R3525 T12310 T12308 pobj gene,of
R3526 T12311 T12310 cc and,gene
R3527 T12312 T12310 conj PS,gene
R3528 T12313 T12314 mark that,are
R3529 T12314 T12304 ccomp are,revealed
R3530 T12315 T12314 nsubj these,are
R3531 T12316 T12314 acomp homologous,are
R3532 T12317 T12316 prep from,homologous
R3533 T12318 T12319 nummod eight,pairs
R3534 T12319 T12317 pobj pairs,from
R3535 T12320 T12319 compound base,pairs
R3536 T12321 T12319 advmod upstream,pairs
R3537 T12322 T12321 prep of,upstream
R3538 T12323 T12324 det the,exon
R3539 T12324 T12322 pobj exon,of
R3540 T12325 T12324 amod first,exon
R3541 T12326 T12317 prep to,from
R3542 T12327 T12328 nummod 675,bp
R3543 T12328 T12326 pobj bp,to
R3544 T12329 T12328 advmod downstream,bp
R3545 T12330 T12329 prep of,downstream
R3546 T12331 T12332 det the,exon
R3547 T12332 T12330 pobj exon,of
R3548 T12333 T12332 amod third,exon
R3549 T12334 T12304 punct .,revealed
R3550 T12336 T12337 compound Substitution,mutations
R3551 T12337 T12338 nsubjpass mutations,indicated
R3552 T12339 T12338 auxpass are,indicated
R3553 T12340 T12338 prep by,indicated
R3554 T12341 T12342 amod black,lines
R3555 T12342 T12340 pobj lines,by
R3556 T12343 T12338 punct .,indicated
R3557 T12345 T12346 det The,insertion
R3558 T12346 T12347 nsubjpass insertion,indicated
R3559 T12348 T12347 auxpass is,indicated
R3560 T12349 T12347 prep by,indicated
R3561 T12350 T12351 det an,triangle
R3562 T12351 T12349 pobj triangle,by
R3563 T12352 T12351 amod open,triangle
R3564 T12353 T12347 punct .,indicated
R3565 T12543 T12544 compound Promoter,enhancer
R3566 T12544 T12546 compound enhancer,activity
R3567 T12545 T12544 punct /,enhancer
R3568 T12547 T12546 prep of,activity
R3569 T12548 T12549 det the,gene
R3570 T12549 T12547 pobj gene,of
R3571 T12550 T12549 compound ESG1,gene
R3572 T12551 T12549 cc and,gene
R3573 T12552 T12549 conj pseudogene,gene
R3574 T12553 T12546 punct .,activity
R3575 T12555 T12556 compound DNA,fragments
R3576 T12556 T12557 nsubjpass fragments,transferred
R3577 T12558 T12556 prep of,fragments
R3578 T12559 T12560 punct ~,6
R3579 T12560 T12561 nummod 6,kbp
R3580 T12561 T12558 pobj kbp,of
R3581 T12562 T12561 acl isolated,kbp
R3582 T12563 T12562 prep from,isolated
R3583 T12564 T12565 det the,regions
R3584 T12565 T12563 pobj regions,from
R3585 T12566 T12565 nummod 5,regions
R3586 T12567 T12566 punct ',5
R3587 T12568 T12565 compound flanking,regions
R3588 T12569 T12565 prep of,regions
R3589 T12570 T12571 det the,gene
R3590 T12571 T12569 pobj gene,of
R3591 T12572 T12571 cc and,gene
R3592 T12573 T12571 conj PS,gene
R3593 T12574 T12557 auxpass were,transferred
R3594 T12575 T12557 prep into,transferred
R3595 T12576 T12577 compound luciferase,reporter
R3596 T12577 T12578 compound reporter,plasmids
R3597 T12578 T12575 pobj plasmids,into
R3598 T12579 T12557 punct .,transferred
R3599 T12581 T12582 nsubj We,introduced
R3600 T12583 T12584 det the,genes
R3601 T12584 T12582 dobj genes,introduced
R3602 T12585 T12584 compound reporter,genes
R3603 T12586 T12582 prep into,introduced
R3604 T12587 T12588 amod undifferentiated,cells
R3605 T12588 T12586 pobj cells,into
R3606 T12589 T12588 compound ES,cells
R3607 T12590 T12591 punct (,columns
R3608 T12591 T12588 parataxis columns,cells
R3609 T12592 T12591 amod open,columns
R3610 T12593 T12591 punct ),columns
R3611 T12594 T12588 punct ", ",cells
R3612 T12595 T12596 amod retinoic,acid
R3613 T12596 T12597 npadvmod acid,treated
R3614 T12597 T12599 amod treated,cells
R3615 T12598 T12597 punct -,treated
R3616 T12599 T12588 conj cells,cells
R3617 T12600 T12599 compound ES,cells
R3618 T12601 T12602 punct (,columns
R3619 T12602 T12599 parataxis columns,cells
R3620 T12603 T12602 amod grey,columns
R3621 T12604 T12602 punct ),columns
R3622 T12605 T12599 punct ", ",cells
R3623 T12606 T12599 cc and,cells
R3624 T12607 T12608 compound NIH3T3,cells
R3625 T12608 T12599 conj cells,cells
R3626 T12609 T12610 punct (,columns
R3627 T12610 T12608 parataxis columns,cells
R3628 T12611 T12610 amod closed,columns
R3629 T12612 T12610 punct ),columns
R3630 T12613 T12582 punct .,introduced
R3631 T12615 T12616 nsubj Data,represent
R3632 T12617 T12618 det the,averages
R3633 T12618 T12616 dobj averages,represent
R3634 T12619 T12618 cc and,averages
R3635 T12620 T12621 amod standard,deviations
R3636 T12621 T12618 conj deviations,averages
R3637 T12622 T12618 prep of,averages
R3638 T12623 T12624 nummod three,experiments
R3639 T12624 T12622 pobj experiments,of
R3640 T12625 T12624 amod independent,experiments
R3641 T12626 T12616 punct .,represent
R3642 T13078 T13079 amod Targeted,disruption
R3643 T13080 T13079 prep of,disruption
R3644 T13081 T13082 det the,gene
R3645 T13082 T13080 pobj gene,of
R3646 T13083 T13082 compound mouse,gene
R3647 T13084 T13082 compound ESG1,gene
R3648 T13085 T13079 punct .,disruption
R3649 T13087 T13088 meta A,strategy
R3650 T13089 T13087 punct ),A
R3651 T13090 T13088 compound Targeting,strategy
R3652 T13091 T13088 punct .,strategy
R3653 T13093 T13094 amod Homologous,regions
R3654 T13094 T13095 nsubjpass regions,indicated
R3655 T13096 T13095 auxpass are,indicated
R3656 T13097 T13095 agent by,indicated
R3657 T13098 T13099 amod thick,lines
R3658 T13099 T13097 pobj lines,by
R3659 T13100 T13095 punct .,indicated
R3660 T13102 T13103 compound Recognition,sites
R3661 T13103 T13104 nsubjpass sites,shown
R3662 T13105 T13103 prep of,sites
R3663 T13106 T13105 pobj PstI,of
R3664 T13107 T13108 punct (,P
R3665 T13108 T13106 parataxis P,PstI
R3666 T13109 T13108 punct ),P
R3667 T13110 T13106 cc and,PstI
R3668 T13111 T13106 conj SpeI,PstI
R3669 T13112 T13113 punct (,S
R3670 T13113 T13111 parataxis S,SpeI
R3671 T13114 T13113 punct ),S
R3672 T13115 T13103 punct ", ",sites
R3673 T13116 T13117 dep which,used
R3674 T13117 T13103 relcl used,sites
R3675 T13118 T13117 auxpass were,used
R3676 T13119 T13117 prep for,used
R3677 T13120 T13121 compound Southern,blot
R3678 T13121 T13122 compound blot,analyses
R3679 T13122 T13119 pobj analyses,for
R3680 T13123 T13104 punct ", ",shown
R3681 T13124 T13104 auxpass are,shown
R3682 T13125 T13104 punct .,shown
R3683 T13127 T13128 det The,A
R3684 T13128 T13133 nsubjpass A,inserted
R3685 T13129 T13128 nmod gene,A
R3686 T13130 T13128 amod encoding,A
R3687 T13131 T13128 compound diphtheria,A
R3688 T13132 T13128 compound toxin,A
R3689 T13134 T13128 punct (,A
R3690 T13135 T13128 appos DTA,A
R3691 T13136 T13133 punct ),inserted
R3692 T13137 T13133 auxpass was,inserted
R3693 T13138 T13133 prep at,inserted
R3694 T13139 T13140 det the,end
R3695 T13140 T13138 pobj end,at
R3696 T13141 T13140 nummod 3,end
R3697 T13142 T13141 punct ',3
R3698 T13143 T13140 prep of,end
R3699 T13144 T13145 det the,vectors
R3700 T13145 T13143 pobj vectors,of
R3701 T13146 T13145 compound targeting,vectors
R3702 T13147 T13148 aux to,facilitate
R3703 T13148 T13133 advcl facilitate,inserted
R3704 T13149 T13150 amod negative,selection
R3705 T13150 T13148 dobj selection,facilitate
R3706 T13151 T13133 punct .,inserted
R3707 T13153 T13154 meta B,analyses
R3708 T13155 T13153 punct ),B
R3709 T13156 T13157 compound Southern,blot
R3710 T13157 T13154 compound blot,analyses
R3711 T13158 T13154 acl confirming,analyses
R3712 T13159 T13160 amod homologous,recombination
R3713 T13160 T13158 dobj recombination,confirming
R3714 T13161 T13154 punct .,analyses
R3715 T13164 T13163 punct ", ",WT
R3716 T13165 T13166 amod wild,type
R3717 T13166 T13168 compound type,cells
R3718 T13167 T13166 punct -,type
R3719 T13168 T13163 appos cells,WT
R3720 T13169 T13168 compound ES,cells
R3721 T13170 T13163 punct ;,WT
R3722 T13171 T13163 appos β,WT
R3723 T13172 T13171 punct ", ",β
R3724 T13173 T13174 nmod β,geo
R3725 T13174 T13176 nmod geo,cells
R3726 T13175 T13174 punct -,geo
R3727 T13176 T13171 appos cells,β
R3728 T13177 T13174 punct +,geo
R3729 T13178 T13174 punct /,geo
R3730 T13179 T13174 punct -,geo
R3731 T13180 T13176 compound ES,cells
R3732 T13181 T13163 punct ;,WT
R3733 T13182 T13163 appos H,WT
R3734 T13183 T13182 punct ", ",H
R3735 T13184 T13185 nmod HygR,cells
R3736 T13185 T13182 appos cells,H
R3737 T13186 T13184 punct +,HygR
R3738 T13187 T13184 punct /,HygR
R3739 T13188 T13184 punct -,HygR
R3740 T13189 T13185 compound ES,cells
R3741 T13190 T13163 punct ;,WT
R3742 T13191 T13192 punct -,-
R3743 T13192 T13163 appos -,WT
R3744 T13193 T13192 punct /,-
R3745 T13194 T13192 punct ", ",-
R3746 T13195 T13196 npadvmod ESG1,null
R3747 T13196 T13198 amod null,cells
R3748 T13197 T13196 punct -,null
R3749 T13198 T13192 appos cells,-
R3750 T13199 T13198 compound ES,cells
R3751 T13200 T13163 punct .,WT
R3752 T13202 T13203 nsubj Numbers,indicate
R3753 T13204 T13205 compound clone,numbers
R3754 T13205 T13203 dobj numbers,indicate
R3755 T13206 T13203 punct .,indicate
R3756 T13208 T13209 meta C,analyses
R3757 T13210 T13208 punct ),C
R3758 T13211 T13212 nmod Northern,blot
R3759 T13212 T13209 nmod blot,analyses
R3760 T13213 T13214 punct (,upper
R3761 T13214 T13212 parataxis upper,blot
R3762 T13215 T13214 punct ),upper
R3763 T13216 T13212 cc and,blot
R3764 T13217 T13218 compound western,blot
R3765 T13218 T13212 conj blot,blot
R3766 T13219 T13220 punct (,lower
R3767 T13220 T13218 parataxis lower,blot
R3768 T13221 T13220 punct ),lower
R3769 T13222 T13209 prep of,analyses
R3770 T13223 T13224 amod wild,type
R3771 T13224 T13226 compound type,cells
R3772 T13225 T13224 punct -,type
R3773 T13226 T13222 pobj cells,of
R3774 T13227 T13226 compound ES,cells
R3775 T13228 T13229 punct (,WT
R3776 T13229 T13226 parataxis WT,cells
R3777 T13230 T13229 punct ),WT
R3778 T13231 T13226 punct ", ",cells
R3779 T13232 T13233 npadvmod ESG1,null
R3780 T13233 T13235 amod null,cells
R3781 T13234 T13233 punct -,null
R3782 T13235 T13226 conj cells,cells
R3783 T13236 T13235 compound ES,cells
R3784 T13237 T13238 punct (,clones
R3785 T13238 T13235 parataxis clones,cells
R3786 T13239 T13240 punct -,-
R3787 T13240 T13238 punct -,clones
R3788 T13241 T13240 punct /,-
R3789 T13242 T13238 punct ", ",clones
R3790 T13243 T13238 nummod three,clones
R3791 T13244 T13238 punct ),clones
R3792 T13245 T13235 cc and,cells
R3793 T13246 T13247 amod heterozygous,cells
R3794 T13247 T13235 conj cells,cells
R3795 T13248 T13247 compound ES,cells
R3796 T13249 T13250 punct (,-
R3797 T13250 T13247 punct -,cells
R3798 T13251 T13250 punct +,-
R3799 T13252 T13250 punct /,-
R3800 T13253 T13250 punct ),-
R3801 T13254 T13209 punct .,analyses
R3802 T13256 T13257 compound Northern,blot
R3803 T13257 T13258 nsubjpass blot,performed
R3804 T13259 T13258 auxpass was,performed
R3805 T13260 T13261 mark as,described
R3806 T13261 T13258 advcl described,performed
R3807 T13262 T13261 advmod previously,described
R3808 T13263 T13264 punct [,20
R3809 T13264 T13261 parataxis 20,described
R3810 T13265 T13264 punct ],20
R3811 T13266 T13258 punct .,performed
R3812 T13268 T13269 aux To,confirm
R3813 T13269 T13270 advcl confirm,shown
R3814 T13271 T13272 det the,loading
R3815 T13272 T13269 dobj loading,confirm
R3816 T13273 T13272 prep of,loading
R3817 T13274 T13275 amod equal,amounts
R3818 T13275 T13273 pobj amounts,of
R3819 T13276 T13275 prep of,amounts
R3820 T13277 T13276 pobj RNA,of
R3821 T13278 T13270 punct ", ",shown
R3822 T13279 T13280 compound ethidium,bromide
R3823 T13280 T13281 compound bromide,staining
R3824 T13281 T13270 nsubjpass staining,shown
R3825 T13282 T13281 prep of,staining
R3826 T13283 T13284 amod ribosomal,RNA
R3827 T13284 T13282 pobj RNA,of
R3828 T13285 T13270 auxpass is,shown
R3829 T13286 T13270 advmod also,shown
R3830 T13287 T13288 punct (,middle
R3831 T13288 T13270 parataxis middle,shown
R3832 T13289 T13288 punct ),middle
R3833 T13290 T13270 punct .,shown
R3834 T13588 T13587 prep of,Analyses
R3835 T13589 T13590 npadvmod ESG1,null
R3836 T13590 T13592 amod null,cells
R3837 T13591 T13590 punct -,null
R3838 T13592 T13588 pobj cells,of
R3839 T13593 T13592 compound ES,cells
R3840 T13594 T13587 punct .,Analyses
R3841 T13596 T13597 meta A,morphology
R3842 T13598 T13596 punct ),A
R3843 T13599 T13597 det The,morphology
R3844 T13600 T13597 prep of,morphology
R3845 T13601 T13602 npadvmod ESG1,null
R3846 T13602 T13604 amod null,colonies
R3847 T13603 T13602 punct -,null
R3848 T13604 T13600 pobj colonies,of
R3849 T13605 T13604 compound ES,colonies
R3850 T13606 T13604 compound cell,colonies
R3851 T13607 T13597 acl grown,morphology
R3852 T13608 T13607 prep on,grown
R3853 T13609 T13610 compound STO,cells
R3854 T13610 T13608 pobj cells,on
R3855 T13611 T13610 compound feeder,cells
R3856 T13612 T13597 punct .,morphology
R3857 T13614 T13615 meta B,curve
R3858 T13616 T13614 punct ),B
R3859 T13617 T13615 compound Growth,curve
R3860 T13618 T13615 prep of,curve
R3861 T13619 T13620 amod wild,type
R3862 T13620 T13622 nmod type,cells
R3863 T13621 T13620 punct -,type
R3864 T13622 T13618 pobj cells,of
R3865 T13623 T13620 punct (,type
R3866 T13624 T13620 appos WT,type
R3867 T13625 T13620 punct ),type
R3868 T13626 T13620 punct ", ",type
R3869 T13627 T13620 conj ESG1,type
R3870 T13628 T13627 punct -,ESG1
R3871 T13629 T13627 amod null,ESG1
R3872 T13630 T13627 punct (,ESG1
R3873 T13631 T13632 punct -,-
R3874 T13632 T13627 appos -,ESG1
R3875 T13633 T13632 punct /,-
R3876 T13634 T13627 punct ),ESG1
R3877 T13635 T13627 cc and,ESG1
R3878 T13636 T13627 conj heterozygous,ESG1
R3879 T13637 T13636 punct (,heterozygous
R3880 T13638 T13639 punct +,-
R3881 T13639 T13636 appos -,heterozygous
R3882 T13640 T13639 punct /,-
R3883 T13641 T13622 punct ),cells
R3884 T13642 T13622 compound ES,cells
R3885 T13643 T13615 punct .,curve
R3886 T13645 T13646 det Each,clone
R3887 T13646 T13647 nsubjpass clone,plated
R3888 T13648 T13649 punct (,cells
R3889 T13649 T13646 parataxis cells,clone
R3890 T13650 T13651 quantmod 1,104
R3891 T13651 T13649 nummod 104,cells
R3892 T13652 T13651 punct ×,104
R3893 T13653 T13654 punct /,well
R3894 T13654 T13649 prep well,cells
R3895 T13655 T13649 punct ),cells
R3896 T13656 T13647 auxpass was,plated
R3897 T13657 T13647 prep in,plated
R3898 T13658 T13659 nummod 24,well
R3899 T13659 T13661 compound well,plates
R3900 T13660 T13659 punct -,well
R3901 T13661 T13657 pobj plates,in
R3902 T13662 T13647 punct .,plated
R3903 T13664 T13665 compound Cell,numbers
R3904 T13665 T13666 nsubjpass numbers,determined
R3905 T13667 T13666 auxpass were,determined
R3906 T13668 T13666 prep with,determined
R3907 T13669 T13670 det a,counter
R3908 T13670 T13668 pobj counter,with
R3909 T13671 T13670 compound Coulter,counter
R3910 T13672 T13666 prep at,determined
R3911 T13673 T13674 nummod 2,days
R3912 T13674 T13672 pobj days,at
R3913 T13675 T13673 punct ", ",2
R3914 T13676 T13673 conj 4,2
R3915 T13677 T13676 punct ", ",4
R3916 T13678 T13676 cc and,4
R3917 T13679 T13676 conj 6,4
R3918 T13680 T13666 punct .,determined
R3919 T13682 T13683 nsubjpass Data,shown
R3920 T13684 T13682 prep of,Data
R3921 T13685 T13686 punct +,-
R3922 T13686 T13688 punct -,cells
R3923 T13687 T13686 punct /,-
R3924 T13688 T13684 pobj cells,of
R3925 T13689 T13686 cc and,-
R3926 T13690 T13691 punct -,-
R3927 T13691 T13686 conj -,-
R3928 T13692 T13691 punct /,-
R3929 T13693 T13683 auxpass are,shown
R3930 T13694 T13683 prep as,shown
R3931 T13695 T13694 pobj averages,as
R3932 T13696 T13695 cc and,averages
R3933 T13697 T13698 amod standard,deviations
R3934 T13698 T13695 conj deviations,averages
R3935 T13699 T13695 prep of,averages
R3936 T13700 T13701 nummod three,clones
R3937 T13701 T13699 pobj clones,of
R3938 T13702 T13701 amod independent,clones
R3939 T13703 T13683 punct .,shown
R3940 T13705 T13706 meta C,section
R3941 T13707 T13705 punct ),C
R3942 T13708 T13706 det A,section
R3943 T13709 T13706 prep of,section
R3944 T13710 T13709 pobj teratoma,of
R3945 T13711 T13710 acl derived,teratoma
R3946 T13712 T13711 prep from,derived
R3947 T13713 T13714 npadvmod ESG1,null
R3948 T13714 T13716 amod null,cells
R3949 T13715 T13714 punct -,null
R3950 T13716 T13712 pobj cells,from
R3951 T13717 T13716 compound ES,cells
R3952 T13718 T13719 punct (,staining
R3953 T13719 T13711 parataxis staining,derived
R3954 T13720 T13719 nmod hematoxylin,staining
R3955 T13721 T13720 cc &,hematoxylin
R3956 T13722 T13720 conj eosin,hematoxylin
R3957 T13723 T13719 punct ),staining
R3958 T13724 T13706 punct .,section
R3959 T13961 T13962 compound Gene,expression
R3960 T13962 T13963 compound expression,analyses
R3961 T13964 T13963 prep of,analyses
R3962 T13965 T13966 npadvmod ESG1,null
R3963 T13966 T13968 amod null,cells
R3964 T13967 T13966 punct -,null
R3965 T13968 T13964 pobj cells,of
R3966 T13969 T13968 compound ES,cells
R3967 T13970 T13963 punct .,analyses
R3968 T13972 T13973 meta A,analyses
R3969 T13974 T13972 punct ),A
R3970 T13975 T13973 compound DNA,analyses
R3971 T13976 T13973 compound microarray,analyses
R3972 T13977 T13973 punct .,analyses
R3973 T13979 T13980 amod Total,RNA
R3974 T13980 T13981 nsubjpass RNA,labeled
R3975 T13982 T13980 prep from,RNA
R3976 T13983 T13984 amod wild,type
R3977 T13984 T13986 compound type,cells
R3978 T13985 T13984 punct -,type
R3979 T13986 T13982 pobj cells,from
R3980 T13987 T13986 compound ES,cells
R3981 T13988 T13986 cc and,cells
R3982 T13989 T13990 npadvmod ESG1,null
R3983 T13990 T13992 amod null,cells
R3984 T13991 T13990 punct -,null
R3985 T13992 T13986 conj cells,cells
R3986 T13993 T13992 compound ES,cells
R3987 T13994 T13981 auxpass were,labeled
R3988 T13995 T13981 prep with,labeled
R3989 T13996 T13995 pobj Cy3,with
R3990 T13997 T13996 cc and,Cy3
R3991 T13998 T13996 conj Cy5,Cy3
R3992 T13999 T13981 punct ", ",labeled
R3993 T14000 T13981 advmod respectively,labeled
R3994 T14001 T13981 punct .,labeled
R3995 T14003 T14004 nsubjpass Samples,hybridized
R3996 T14005 T14004 auxpass were,hybridized
R3997 T14006 T14004 prep to,hybridized
R3998 T14007 T14008 nmod Agilent,Microarrays
R3999 T14008 T14006 pobj Microarrays,to
R4000 T14009 T14008 nmod Mouse,Microarrays
R4001 T14010 T14008 amod Developmental,Microarrays
R4002 T14011 T14004 punct .,hybridized
R4003 T14013 T14014 det The,averages
R4004 T14014 T14015 nsubjpass averages,shown
R4005 T14016 T14014 prep of,averages
R4006 T14017 T14018 nummod two,clones
R4007 T14018 T14016 pobj clones,of
R4008 T14019 T14018 amod independent,clones
R4009 T14020 T14015 auxpass are,shown
R4010 T14021 T14015 punct .,shown
R4011 T14023 T14024 meta B,analyses
R4012 T14025 T14023 punct ),B
R4013 T14026 T14027 compound Western,blot
R4014 T14027 T14024 compound blot,analyses
R4015 T14028 T14024 punct .,analyses
R4016 T14030 T14031 compound Cell,lysates
R4017 T14031 T14032 nsubjpass lysates,examined
R4018 T14033 T14031 prep from,lysates
R4019 T14034 T14035 nmod ESG1,cells
R4020 T14035 T14033 pobj cells,from
R4021 T14036 T14034 punct +,ESG1
R4022 T14037 T14034 punct /,ESG1
R4023 T14038 T14034 punct -,ESG1
R4024 T14039 T14034 cc and,ESG1
R4025 T14040 T14034 conj ESG1,ESG1
R4026 T14041 T14040 punct -,ESG1
R4027 T14042 T14040 punct /,ESG1
R4028 T14043 T14040 punct -,ESG1
R4029 T14044 T14032 auxpass were,examined
R4030 T14045 T14032 prep for,examined
R4031 T14046 T14045 pobj expression,for
R4032 T14047 T14046 prep of,expression
R4033 T14048 T14047 pobj ESG1,of
R4034 T14049 T14048 punct ", ",ESG1
R4035 T14050 T14048 conj Oct3,ESG1
R4036 T14051 T14050 punct /,Oct3
R4037 T14052 T14050 nummod 4,Oct3
R4038 T14053 T14050 cc and,Oct3
R4039 T14054 T14050 conj CDK4,Oct3
R4040 T14055 T14032 prep with,examined
R4041 T14056 T14055 pobj immunoblotting,with
R4042 T14057 T14032 punct .,examined
R828 T4123 T4124 compound ESG1,cDNA
R829 T4124 T4122 pobj cDNA,to
R830 T4125 T4119 punct ", ",produce
R831 T4126 T4119 nsubj they,produce
R832 T4127 T4119 aux could,produce
R833 T4128 T4119 neg not,produce
R834 T4129 T4130 amod functional,proteins
R835 T4130 T4119 dobj proteins,produce
R836 T4131 T4119 punct ", ",produce
R837 T4132 T4119 prep because,produce
R838 T4133 T4132 pcomp of,because
R839 T4134 T4135 amod critical,mutations
R840 T4135 T4132 pobj mutations,because
R841 T4136 T4119 punct .,produce
R842 T4138 T4139 det This,result
R843 T4139 T4140 nsubj result,suggests
R844 T4141 T4142 mark that,are
R845 T4142 T4140 ccomp are,suggests
R846 T4143 T4142 expl there,are
R847 T4144 T4145 det a,number
R848 T4145 T4142 attr number,are
R849 T4146 T4145 amod larger,number
R850 T4147 T4145 prep of,number
R851 T4148 T4149 amod intron-less,pseudogenes
R852 T4149 T4147 pobj pseudogenes,of
R853 T4150 T4151 mark than,anticipated
R854 T4151 T4145 advcl anticipated,number
R855 T4152 T4151 advmod previously,anticipated
R856 T4153 T4140 punct .,suggests
R857 T4155 T4156 nsubj Existence,is
R858 T4157 T4155 prep of,Existence
R859 T4158 T4159 amod multiple,retropseudogenes
R860 T4159 T4157 pobj retropseudogenes,of
R861 T4160 T4161 det a,hallmark
R1118 T4442 T4440 ccomp is,concluded
R1119 T4443 T4444 det this,sequence
R1120 T4444 T4442 nsubj sequence,is
R1121 T4445 T4446 det the,gene
R1122 T4446 T4442 attr gene,is
R1123 T4447 T4446 compound ESG1,gene
R1124 T4448 T4440 punct .,concluded
R1141 T4450 T4451 nsubj We,found
R1144 T4452 T4451 advmod also,found
R1145 T4453 T4454 mark that,contained
R1150 T4455 T4456 det the,clones
R1153 T4457 T4456 nummod two,clones
R1154 T4458 T4456 compound BAC,clones
R1375 T6488 T6485 punct ),geo
R1376 T6489 T6472 cc or,fusion
R1377 T6490 T6491 det the,gene
R1378 T6491 T6472 conj gene,fusion
R1379 T6492 T6493 npadvmod hygromycin,resistant
R1380 T6493 T6491 amod resistant,gene
R1381 T6494 T6495 punct (,HygR
R1382 T6495 T6491 parataxis HygR,gene
R1383 T6496 T6495 punct ),HygR
R1384 T6497 T6466 advcl using,replaced
R1385 T6498 T6499 nummod two,vectors
R1445 T6582 T6583 det t,an
R1446 T6588 T6587 compound ES,cells
R1447 T6589 T6586 punct ", ",introduced
R1448 T6590 T6586 nsubj we,introduced
R1449 T6591 T6592 det the,vector
R1450 T6592 T6586 dobj vector,introduced
R1451 T6593 T6594 compound β,geo
R1452 T6594 T6592 compound geo,vector
R1453 T6595 T6594 punct -,geo
R1454 T6596 T6586 prep into,introduced
R1455 T6597 T6598 npadvmod HygR,heterozygous
R1456 T6598 T6599 amod heterozygous,cells
R1457 T6599 T6596 pobj cells,into
R1458 T6600 T6599 compound ES,cells
R1459 T6601 T6586 punct .,introduced
R1460 T6603 T6604 prep Of,were
R1461 T6605 T6606 nummod 105,colonies
R1462 T6606 T6603 pobj colonies,Of
R1463 T6607 T6608 npadvmod G418,resistant
R1464 T6608 T6606 amod resistant,colonies
R1465 T6609 T6608 punct -,resistant
R1466 T6610 T6606 acl tested,colonies
R1467 T6611 T6604 punct ", ",were
R1468 T6612 T6604 nsubj 49,were
R1585 T6741 T6742 amod retinoic,treatment
R1586 T6742 T6731 conj treatment,removal
R1587 T6743 T6742 compound acid,treatment
R1588 T6744 T6745 punct (,shown
R1589 T6745 T6742 parataxis shown,treatment
R1590 T6746 T6745 neg not,shown
R1591 T6747 T6745 punct ),shown
R1592 T6748 T6727 punct .,differentiated
R1593 T6750 T6751 advmod When,transplanted
R1594 T6751 T6752 advcl transplanted,produced
R1595 T6753 T6751 prep into,transplanted
R1596 T6754 T6755 compound hind,flanks
R1597 T6755 T6753 pobj flanks,into
R1598 T6756 T6755 prep of,flanks
R1599 T6757 T6758 amod nude,mice
R1600 T6758 T6756 pobj mice,of
R1601 T6759 T6752 punct ", ",produced
R1602 T6760 T6761 det these,cells
R1603 T6761 T6752 nsubj cells,produced
R1604 T6762 T6752 dobj teratomas,produced
R1605 T6763 T6762 punct ", ",teratomas
R1606 T6764 T6762 appos tumors,teratomas
R1607 T6765 T6764 acl containing,tumors
R1608 T6766 T6765 dobj components,containing
R1609 T6767 T6766 prep of,components
R1610 T6768 T6769 det all,layers
R1611 T6769 T6767 pobj layers,of
R1612 T6770 T6769 nummod three,layers
R1613 T6771 T6769 compound germ,layers
R1637 T6804 T6803 nmod x,a
R1638 T6807 T6808 det the,profiles
R1639 T6808 T6806 dobj profiles,mined
R1640 T6809 T6808 compound gene,profiles
R1641 T6810 T6808 compound expression,profiles
R1642 T6811 T6808 prep of,profiles
R1643 T6812 T6813 nmod ESG1,cells
R1644 T6813 T6811 pobj cells,of
R1645 T6814 T6812 punct -,ESG1
R1646 T6815 T6812 punct /,ESG1
R1647 T6816 T6812 punct -,ESG1
R1648 T6817 T6813 compound ES,cells
R1649 T6818 T6806 advcl using,mined
R1650 T6819 T6820 npadvmod oligonucleotide,based
R1651 T6820 T6822 amod based,microarrays
R1652 T6821 T6820 punct -,based
R1783 T6964 T6963 cc or,directly
R1784 T6965 T6963 conj indirectly,directly
R1785 T6966 T6960 agent by,regulated
R1786 T6967 T6966 pobj ESG1,by
R1787 T6968 T6960 punct .,regulated
R1788 T6970 T6971 mark Since,contains
R1789 T6971 T6973 advcl contains,stabilize
R1790 T6972 T6971 nsubj ESG1,contains
R1791 T6974 T6975 det a,domain
R1792 T6975 T6971 dobj domain,contains
R1793 T6976 T6977 nmod KH,type
R1794 T6977 T6975 nmod type,domain
R1795 T6978 T6977 punct -,type
R1796 T6979 T6980 npadvmod RNA,binding
R1797 T6980 T6975 amod binding,domain
R1798 T6981 T6980 punct -,binding
R1799 T6982 T6973 punct ", ",stabilize
R1800 T6983 T6973 nsubj it,stabilize
R1801 T6984 T6973 aux may,stabilize
R1802 T6985 T6973 dobj mRNA,stabilize
R1803 T6986 T6985 prep of,mRNA
R1804 T6987 T6988 det these,genes
R1805 T6988 T6986 pobj genes,of
R1806 T6989 T6973 punct .,stabilize
R1807 T6991 T6992 advmod Further,studied
R1808 T6992 T6993 nsubjpass studied,required
R1809 T6994 T6993 auxpass are,required
R1810 T6995 T6996 aux to,clarify
R1811 T6996 T6993 advcl clarify,required
R1812 T6997 T6998 det this,possibility
R1813 T6998 T6996 dobj possibility,clarify
R1814 T6999 T6993 punct .,required
R1815 T7004 T7005 nmod gen,ra
R1816 T7006 T7005 nmod e,ra
R1817 T7012 T7011 punct -,ESG1
R1818 T7015 T7016 compound knockout,mice
R1819 T7017 T7014 punct ", ",injected
R1820 T7018 T7014 nsubj we,injected
R1821 T7019 T7020 nmod β,ESG1
R1822 T7020 T7024 nmod ESG1,clones
R1823 T7021 T7020 punct -,ESG1
R1824 T7022 T7020 nmod geo,ESG1
R1825 T7023 T7020 punct -,ESG1
R1826 T7024 T7014 dobj clones,injected
R1827 T7025 T7020 punct +,ESG1
R1828 T7026 T7020 punct /,ESG1
R1829 T7027 T7020 punct -,ESG1
R1830 T7028 T7024 compound ES,clones
R1831 T7029 T7024 compound cell,clones
R1832 T7030 T7014 prep into,injected
R1833 T7031 T7032 det the,blastocysts
R1834 T7032 T7030 pobj blastocysts,into
R1835 T7033 T7032 prep of,blastocysts
R1836 T7034 T7035 compound C57BL6,mice
R1837 T7035 T7033 pobj mice,of
R1838 T7036 T7014 punct .,injected
R1839 T7038 T7039 nsubj We,obtained
R1840 T7040 T7041 compound germline,transmission
R1841 T7041 T7039 dobj transmission,obtained
R1842 T7042 T7039 prep from,obtained
R1843 T7043 T7044 nummod three,clones
R1844 T7044 T7042 pobj clones,from
R1845 T7045 T7039 punct .,obtained
R1846 T7047 T7048 nsubj We,obtained
R1847 T7049 T7050 nmod ESG1,mice
R1848 T7050 T7048 dobj mice,obtained
R1849 T7051 T7049 punct -,ESG1
R1850 T7052 T7049 punct /,ESG1
R1851 T7053 T7049 punct -,ESG1
R1852 T7054 T7048 prep at,obtained
R1853 T7055 T7056 det the,ratios
R1854 T7056 T7054 pobj ratios,at
R1855 T7057 T7056 amod Mendelian,ratios
R1856 T7058 T7056 punct (,ratios