PMC:1420271 / 18989-19129 JSONTXT

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    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T10372","span":{"begin":18,"end":22},"obj":"PR:000006847"},{"id":"T10373","span":{"begin":45,"end":66},"obj":"CHEBI:44557"},{"id":"T10374","span":{"begin":67,"end":74},"obj":"CHEBI:2511"},{"id":"T10375","span":{"begin":135,"end":139},"obj":"CHEBI:16199"}],"text":" Histidine-tagged ESG1 was purified using Ni-nitrilotriacetic acid agarose (Qiagen) under denaturing conditions in the presence of 8 M urea."}

    craft-ca-core-ex-dev

    {"project":"craft-ca-core-ex-dev","denotations":[{"id":"T10400","span":{"begin":1,"end":10},"obj":"CHEBI_SO_EXT:histidine"},{"id":"T10401","span":{"begin":11,"end":17},"obj":"CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process"},{"id":"T10402","span":{"begin":18,"end":22},"obj":"PR_EXT:000006847"},{"id":"T10403","span":{"begin":42,"end":44},"obj":"CHEBI_EXT:nickel"},{"id":"T10404","span":{"begin":45,"end":66},"obj":"CHEBI:44557"},{"id":"T10405","span":{"begin":67,"end":74},"obj":"CHEBI:2511"},{"id":"T10406","span":{"begin":90,"end":100},"obj":"GO_EXT:macromolecule_denaturation"},{"id":"T10407","span":{"begin":135,"end":139},"obj":"CHEBI:16199"}],"text":" Histidine-tagged ESG1 was purified using Ni-nitrilotriacetic acid agarose (Qiagen) under denaturing conditions in the presence of 8 M urea."}

    craft-sa-dev

    {"project":"craft-sa-dev","denotations":[{"id":"T10529","span":{"begin":0,"end":140},"obj":"sentence"},{"id":"T10530","span":{"begin":1,"end":10},"obj":"NN"},{"id":"T10531","span":{"begin":11,"end":17},"obj":"VBN"},{"id":"T10532","span":{"begin":10,"end":11},"obj":"HYPH"},{"id":"T10533","span":{"begin":18,"end":22},"obj":"NN"},{"id":"T10534","span":{"begin":27,"end":35},"obj":"VBN"},{"id":"T10535","span":{"begin":23,"end":26},"obj":"VBD"},{"id":"T10536","span":{"begin":36,"end":41},"obj":"VBG"},{"id":"T10537","span":{"begin":42,"end":44},"obj":"NN"},{"id":"T10538","span":{"begin":67,"end":74},"obj":"NN"},{"id":"T10539","span":{"begin":44,"end":45},"obj":":"},{"id":"T10540","span":{"begin":45,"end":61},"obj":"JJ"},{"id":"T10541","span":{"begin":62,"end":66},"obj":"NN"},{"id":"T10542","span":{"begin":75,"end":76},"obj":"-LRB-"},{"id":"T10543","span":{"begin":76,"end":82},"obj":"NNP"},{"id":"T10544","span":{"begin":82,"end":83},"obj":"-RRB-"},{"id":"T10545","span":{"begin":84,"end":89},"obj":"IN"},{"id":"T10546","span":{"begin":90,"end":100},"obj":"NN"},{"id":"T10547","span":{"begin":101,"end":111},"obj":"NNS"},{"id":"T10548","span":{"begin":112,"end":114},"obj":"IN"},{"id":"T10549","span":{"begin":115,"end":118},"obj":"DT"},{"id":"T10550","span":{"begin":119,"end":127},"obj":"NN"},{"id":"T10551","span":{"begin":128,"end":130},"obj":"IN"},{"id":"T10552","span":{"begin":131,"end":132},"obj":"CD"},{"id":"T10553","span":{"begin":133,"end":134},"obj":"NN"},{"id":"T10554","span":{"begin":135,"end":139},"obj":"NN"},{"id":"T10555","span":{"begin":139,"end":140},"obj":"."}],"relations":[{"id":"R2997","pred":"npadvmod","subj":"T10530","obj":"T10531"},{"id":"R2998","pred":"amod","subj":"T10531","obj":"T10533"},{"id":"R2999","pred":"punct","subj":"T10532","obj":"T10531"},{"id":"R3000","pred":"nsubjpass","subj":"T10533","obj":"T10534"},{"id":"R3001","pred":"auxpass","subj":"T10535","obj":"T10534"},{"id":"R3002","pred":"advcl","subj":"T10536","obj":"T10534"},{"id":"R3003","pred":"nmod","subj":"T10537","obj":"T10538"},{"id":"R3004","pred":"dobj","subj":"T10538","obj":"T10536"},{"id":"R3005","pred":"punct","subj":"T10539","obj":"T10537"},{"id":"R3006","pred":"amod","subj":"T10540","obj":"T10537"},{"id":"R3007","pred":"compound","subj":"T10541","obj":"T10538"},{"id":"R3008","pred":"punct","subj":"T10542","obj":"T10543"},{"id":"R3009","pred":"parataxis","subj":"T10543","obj":"T10538"},{"id":"R3010","pred":"punct","subj":"T10544","obj":"T10543"},{"id":"R3011","pred":"prep","subj":"T10545","obj":"T10536"},{"id":"R3012","pred":"compound","subj":"T10546","obj":"T10547"},{"id":"R3013","pred":"pobj","subj":"T10547","obj":"T10545"},{"id":"R3014","pred":"prep","subj":"T10548","obj":"T10536"},{"id":"R3015","pred":"det","subj":"T10549","obj":"T10550"},{"id":"R3016","pred":"pobj","subj":"T10550","obj":"T10548"},{"id":"R3017","pred":"prep","subj":"T10551","obj":"T10550"},{"id":"R3018","pred":"nummod","subj":"T10552","obj":"T10553"},{"id":"R3019","pred":"compound","subj":"T10553","obj":"T10554"},{"id":"R3020","pred":"pobj","subj":"T10554","obj":"T10551"},{"id":"R3021","pred":"punct","subj":"T10555","obj":"T10534"}],"text":" Histidine-tagged ESG1 was purified using Ni-nitrilotriacetic acid agarose (Qiagen) under denaturing conditions in the presence of 8 M urea."}