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PMC:1420271 / 1853-21919 JSONTXT

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craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T2001 11-20 UBERON:0000922 denotes Embryonic
T2002 11-25 _FRAGMENT denotes Embryonic stem
T2003 31-36 CL:0002322 denotes cells
T2004 27-29 _FRAGMENT denotes ES
T2005 65-76 UBERON:0000358 denotes blastocysts
T2006 80-84 NCBITaxon:10088 denotes mice
T2007 103-109 NCBITaxon:9606 denotes humans
T2008 123-131 CL:0002322 denotes ES cells
T2009 278-291 GO:0008283 denotes proliferation
T2010 320-325 NCBITaxon:10088 denotes mouse
T2011 326-334 CL:0002322 denotes ES cells
T2012 361-365 SO:0000704 denotes gene
T2013 413-418 NCBITaxon:9606 denotes human
T2014 419-427 CL:0002322 denotes ES cells
T2015 517-528 UBERON:0002240 denotes spinal cord
T2016 624-640 _FRAGMENT denotes proliferation of
T2017 644-649 GO:0008283 denotes cells
T2018 641-649 CL:0002322 denotes ES cells
T2019 694-703 CL:0000034 denotes stem cell
T2020 731-740 CHEBI:36357 denotes molecules
T2021 754-762 CL:0002322 denotes ES cells
T2022 849-854 SO:0000704 denotes genes
T2023 868-877 GO:0010467 denotes expressed
T2024 881-889 CL:0002322 denotes ES cells
T2025 894-907 UBERON:0019248 denotes early embryos
T2026 967-976 GO:0010467 denotes expressed
T2027 967-989 SO:0000345 denotes expressed sequence tag
T2028 1033-1044 SO:0000673 denotes transcripts
T2029 1056-1060 PR:000006847 denotes ESG1
T2030 1086-1091 PR:000006847 denotes dppa5
T2031 1095-1100 PR:000006847 denotes ECAT2
T2032 1102-1106 PR:000006847 denotes ESG1
T2033 1138-1148 SO:0000673 denotes transcript
T2034 1149-1153 PR:000006847 denotes Ph34
T2035 1181-1194 CHEBI:26536 denotes retinoic acid
T2036 1198-1207 UBERON:0000922 denotes embryonic
T2037 1198-1207 _FRAGMENT denotes embryonic
T2038 1218-1223 CL:0002321 denotes cells
T2039 1234-1244 GO:0010467 denotes expression
T2040 1253-1257 SO:0000704 denotes gene
T2041 1274-1278 NCBITaxon:10088 denotes mice
T2042 1282-1295 UBERON:0019248 denotes early embryos
T2043 1300-1310 CL:0000586 denotes germ cells
T2050 1406-1417 _FRAGMENT denotes multipotent
T2051 1427-1437 CL:0000048 denotes stem cells
T2052 1418-1437 CL:0000014 denotes germline stem cells
T2053 1444-1448 PR:000006847 denotes ESG1
T2054 1516-1522 SO:0000417 denotes domain
T2055 1548-1554 SO:0000417 denotes domain
T2056 1593-1597 PR:000006847 denotes ESG1
T2057 1659-1667 CL:0002322 denotes ES cells
T2058 1672-1676 NCBITaxon:10088 denotes mice
T2059 1687-1694 SO:0001026 denotes genomic
T2060 1727-1741 SO:0000040 denotes genomic clones
T2061 1757-1762 NCBITaxon:10088 denotes mouse
T2062 1763-1767 PR:000006847 denotes ESG1
T2063 1768-1772 SO:0000704 denotes gene
T2064 1783-1794 SO:0000336 denotes pseudogenes
T2065 1814-1825 SO:0000336 denotes pseudogenes
T2066 1844-1848 SO:0000147 denotes exon
T2067 1849-1855 SO:0000188 denotes intron
T2068 1873-1877 PR:000006847 denotes ESG1
T2069 1878-1882 SO:0000704 denotes gene
T2070 1915-1919 SO:0000704 denotes gene
T2071 1952-1963 SO:0000336 denotes pseudogenes
T2072 1984-1991 SO:0000188 denotes introns
T2073 2033-2051 GO:0032197 denotes retrotransposition
T2074 2055-2059 PR:000006847 denotes ESG1
T2075 2060-2071 SO:0000673 denotes transcripts
T2076 2110-2115 NCBITaxon:10088 denotes mouse
T2077 2116-2120 PR:000006847 denotes ESG1
T2078 2121-2125 SO:0000704 denotes gene
T2079 2130-2141 SO:0000336 denotes pseudogenes
T2080 2226-2231 NCBITaxon:10088 denotes mouse
T2081 2232-2236 SO:0000704 denotes gene
T2082 2271-2282 SO:0000336 denotes pseudogenes
T2083 2302-2306 SO:0000704 denotes gene
T2084 2360-2364 PR:000006847 denotes ESG1
T3792 2434-2439 NCBITaxon:10088 denotes mouse
T3793 2440-2444 PR:000006847 denotes ESG1
T3794 2445-2449 SO:0000704 denotes gene
T3795 2454-2464 SO:0000336 denotes psedogenes
T3796 2515-2520 NCBITaxon:10088 denotes mouse
T3797 2521-2525 PR:000006847 denotes ESG1
T3798 2526-2530 SO:0000704 denotes gene
T3799 2535-2546 SO:0000336 denotes pseudogenes
T3800 2585-2590 NCBITaxon:10088 denotes mouse
T3801 2591-2598 SO:0001026 denotes genomic
T3802 2617-2621 PR:000006847 denotes ESG1
T3803 2679-2690 SO:0000336 denotes pseudogenes
T3804 2699-2706 SO:0000188 denotes introns
T3805 2783-2789 SO:0000188 denotes intron
T3806 2795-2806 SO:0000336 denotes pseudogenes
T3807 2910-2922 SO:0000336 denotes pseudodgenes
T3808 2940-2948 SO:0000857 denotes homology
T3809 2952-2956 PR:000006847 denotes ESG1
T3810 3093-3099 SO:0000188 denotes intron
T3811 3105-3116 SO:0000336 denotes pseudogenes
T3812 3168-3184 SO:0000043 denotes retropseudogenes
T3813 3228-3233 SO:0000704 denotes genes
T3814 3543-3547 PR:000006847 denotes ESG1
T3815 3548-3552 SO:0000704 denotes gene
T3816 3580-3587 SO:0000188 denotes introns
T3817 3621-3626 SO:0000147 denotes exons
T3818 3677-3681 PR:000006847 denotes ESG1
T3819 3716-3720 SO:0000147 denotes exon
T3820 3770-3774 PR:000006847 denotes ESG1
T3821 3775-3779 SO:0000704 denotes gene
T3822 3888-3892 SO:0000147 denotes exon
T3823 3900-3904 PR:000006847 denotes ESG1
T3824 3905-3909 SO:0000704 denotes gene
T3825 3945-3949 PR:000006847 denotes ESG1
T3826 4055-4059 PR:000006847 denotes ESG1
T3827 4060-4064 SO:0000704 denotes gene
T3828 4082-4091 NCBITaxon:2 denotes bacterial
T3829 4082-4113 SO:0000153 denotes bacterial artificial chromosome
T3830 4115-4118 SO:0000153 denotes BAC
T3831 4142-4148 SO:0000112 denotes primer
T3832 4188-4192 SO:0000704 denotes gene
T3833 4202-4213 SO:0000336 denotes pseudogenes
T3834 4260-4263 SO:0000153 denotes BAC
T3835 4396-4400 PR:000006847 denotes ESG1
T3836 4443-4450 SO:0000188 denotes introns
T3837 4472-4478 SO:0000188 denotes intron
T3838 4555-4576 SO:0001421 denotes exon-intron junctions
T3876 6809-6820 SO:0000336 denotes pseudogenes
T3877 6891-6901 SO:0000336 denotes pseudogene
T6124 6931-6936 NCBITaxon:10088 denotes mouse
T6125 6937-6941 PR:000006847 denotes ESG1
T6126 6942-6946 SO:0000704 denotes gene
T6127 6972-6976 PR:000006847 denotes ESG1
T6128 6993-6997 SO:0000704 denotes gene
T6129 7029-7034 NCBITaxon:10088 denotes mouse
T6130 7035-7043 CL:0002322 denotes ES cells
T6131 7067-7072 SO:0000147 denotes exons
T6132 7101-7109 CHEBI:7507 denotes neomycin
T6133 7141-7146 SO:0000704 denotes genes
T6134 7162-7172 CHEBI:24753 denotes hygromycin
T6135 7183-7187 SO:0000704 denotes gene
T6136 7205-7222 SO:0001644 denotes targeting vectors
T6137 7255-7263 CL:0002322 denotes ES cells
T6138 7302-7309 CL:0002322 denotes ES cell
T6139 7368-7384 SO:0001644 denotes targeting vector
T6140 7522-7538 SO:0001644 denotes targeting vector
T6171 10179-10183 SO:0000704 denotes gene
T6172 10218-10222 PR:000006847 denotes ESG1
T6173 10226-10234 CL:0002322 denotes ES cells
T6174 10252-10262 GO:0010467 denotes expression
T6175 10266-10273 CL:0002322 denotes ES cell
T6176 10281-10286 SO:0000704 denotes genes
T6177 10296-10301 PR:000010968 denotes Nanog
T6178 10306-10310 PR:000013044 denotes Oct3
T6179 10328-10332 PR:000006847 denotes ESG1
T6180 10336-10344 CL:0002322 denotes ES cells
T6181 10366-10370 PR:000013044 denotes Oct3
T6182 10373-10383 GO:0010467 denotes expression
T6183 10443-10447 SO:0000704 denotes gene
T6184 10443-10458 GO:0010467 denotes gene expression
T6185 10497-10505 CL:0002322 denotes ES cells
T6186 10510-10514 PR:000006847 denotes ESG1
T6187 10518-10526 CL:0002322 denotes ES cells
T6188 10536-10541 SO:0000704 denotes genes
T6189 10589-10593 PR:000006847 denotes ESG1
T6190 10614-10618 PR:000009450 denotes Krt1
T6191 10622-10625 PR:000014397 denotes Pem
T6192 10627-10631 PR:000003172 denotes Ctgf
T6193 10633-10638 PR:000013428 denotes Ptgs2
T6194 10649-10654 PR:000000216 denotes Inhba
T6195 10662-10667 SO:0000704 denotes genes
T6196 10677-10686 GO:0065007 denotes regulated
T6197 10713-10717 PR:000006847 denotes ESG1
T6198 10725-10729 PR:000006847 denotes ESG1
T6199 10761-10767 SO:0000417 denotes domain
T6200 10800-10805 SO:0000704 denotes genes
T6201 11322-11323 NCBITaxon:10088 denotes e
T6202 11328-11332 PR:000006847 denotes ESG1
T6203 11342-11346 NCBITaxon:10088 denotes mice
T6204 11366-11370 PR:000006847 denotes ESG1
T6205 11374-11381 CL:0002322 denotes ES cell
T6206 11398-11409 UBERON:0000358 denotes blastocysts
T6207 11420-11424 NCBITaxon:10088 denotes mice
T6208 11491-11495 PR:000006847 denotes ESG1
T6209 11499-11503 NCBITaxon:10088 denotes mice
T6210 11546-11550 PR:000006847 denotes ESG1
T6211 11562-11566 PR:000006847 denotes ESG1
T6212 11592-11596 PR:000006847 denotes ESG1
T6213 11600-11604 NCBITaxon:10088 denotes mice
T6214 11612-11619 NCBITaxon:33208 denotes animals
T6215 11723-11729 UBERON:0000473 denotes testis
T6216 11734-11739 UBERON:0000992 denotes ovary
T6217 11819-11823 PR:000006847 denotes ESG1
T6218 11848-11853 NCBITaxon:10088 denotes mouse
T6219 11870-11879 CL:0000586 denotes germ cell
T6220 11870-11889 GO:0007281 denotes germ cell formation
T6221 11909-11917 CL:0002322 denotes ES cells
T6222 11923-11934 UBERON:0000358 denotes blastocysts
T6223 11963-11967 PR:000006847 denotes ESG1
T6224 11981-11985 PR:000006847 denotes ESG1
T6225 12011-12018 CL:0002322 denotes ES cell
T6226 12054-12058 PR:000006847 denotes ESG1
T6227 12069-12073 PR:000006847 denotes ESG1
T6228 12079-12087 CL:0002322 denotes ES cells
T6229 12120-12133 GO:0008283 denotes proliferation
T6230 12184-12188 PR:000006847 denotes ESG1
T6231 12207-12215 CL:0002322 denotes ES cells
T7334 12267-12271 PR:000006847 denotes ESG1
T7335 12291-12296 NCBITaxon:10088 denotes mouse
T7336 12297-12301 SO:0000704 denotes gene
T7337 12364-12372 CL:0002322 denotes ES cells
T7338 12391-12401 GO:0010467 denotes expression
T7339 12405-12418 UBERON:0019248 denotes early embryos
T7340 12420-12430 CL:0000586 denotes germ cells
T7341 12482-12486 PR:000006847 denotes ESG1
T7342 12506-12511 NCBITaxon:10088 denotes mouse
T7343 12525-12534 CL:0000586 denotes germ cell
T7344 12525-12544 GO:0007281 denotes germ cell formation
T7345 12550-12557 CL:0002322 denotes ES cell
T7346 12553-12570 GO:0017145 denotes cell self-renewal
T8140 12613-12616 SO:0000153 denotes BAC
T8141 12639-12644 NCBITaxon:10088 denotes mouse
T8142 12645-12649 PR:000006847 denotes ESG1
T8143 12650-12654 SO:0000704 denotes gene
T8144 12667-12676 NCBITaxon:2 denotes bacterial
T8145 12667-12698 SO:0000153 denotes bacterial artificial chromosome
T8146 12700-12703 SO:0000153 denotes BAC
T8147 12723-12728 NCBITaxon:10088 denotes mouse
T8148 12729-12733 PR:000006847 denotes ESG1
T8149 12734-12738 SO:0000704 denotes gene
T8150 12776-12781 NCBITaxon:10088 denotes mouse
T8151 12782-12785 SO:0000153 denotes BAC
T8152 12834-12838 PR:000006847 denotes pH34
T8153 12877-12881 PR:000006847 denotes pH34
T8154 12916-12923 SO:0000112 denotes primers
T8155 13004-13007 SO:0000153 denotes BAC
T8156 13043-13047 PR:000006847 denotes pH34
T8157 13093-13097 PR:000006847 denotes pH34
T8158 13132-13136 PR:000006847 denotes pH34
T8159 13182-13186 PR:000006847 denotes pH34
T8160 13229-13233 PR:000006847 denotes pH34
T8161 13281-13294 GO:0097617 denotes Hybridization
T8162 13350-13354 PR:000006847 denotes ESG1
T8163 13355-13359 SO:0000704 denotes gene
T8164 13363-13374 SO:0000336 denotes pseudogenes
T8165 13414-13419 NCBITaxon:10088 denotes mouse
T8166 13420-13424 PR:000006847 denotes ESG1
T8167 13425-13429 SO:0000704 denotes gene
T8168 13438-13456 SO:0001417 denotes 3' flanking region
T8169 13478-13480 SO:0000028 denotes bp
T8170 13517-13523 SO:0000440 denotes vector
T8171 13538-13545 PR:P43870 denotes HindIII
T8172 13583-13589 SO:0000440 denotes vector
T8173 13662-13666 PR:000006847 denotes ESG1
T8174 13667-13677 SO:0000336 denotes pseudogene
T8175 13686-13704 SO:0001417 denotes 3' flanking region
T8176 13712-13714 SO:0000028 denotes bp
T8177 13769-13774 PR:P23940 denotes BamHI
T8178 13803-13809 SO:0000440 denotes vector
T8179 13891-13910 SO:0001416 denotes 5' flanking regions
T8180 13918-13922 PR:000006847 denotes ESG1
T8181 13923-13927 SO:0000704 denotes gene
T8182 13944-13955 SO:0000336 denotes pseudogenes
T8183 14005-14009 PR:000006847 denotes pH34
T8184 14057-14061 PR:000006847 denotes pH34
T8185 14067-14074 SO:0000112 denotes primers
T8186 14154-14156 SO:0000028 denotes bp
T8187 14337-14355 SO:0001416 denotes 5' flanking region
T8188 14363-14367 PR:000006847 denotes ESG1
T8189 14368-14372 SO:0000704 denotes gene
T8190 14380-14382 SO:0000028 denotes bp
T8191 14515-14523 SO:0000357 denotes flanking
T8192 14528-14538 SO:0000336 denotes pseudogene
T9096 14557-14561 PR:000006847 denotes ESG1
T9097 14562-14579 SO:0001644 denotes targeting vectors
T9098 14603-14607 PR:000006847 denotes ESG1
T9099 14608-14613 SO:0000147 denotes exons
T9100 14623-14640 SO:0001644 denotes targeting vectors
T9101 14662-14666 SO:0000243 denotes IRES
T9102 14673-14681 SO:0005853 denotes cassette
T9103 14693-14697 SO:0000243 denotes IRES
T9104 14703-14711 SO:0005853 denotes cassette
T9105 14715-14723 SO:0000167 denotes promoter
T9106 14770-14772 SO:0000028 denotes bp
T9107 14807-14811 PR:000006847 denotes pH34
T9108 14865-14869 PR:000006847 denotes pH34
T9109 14923-14930 SO:0000112 denotes primers
T9110 14949-14951 SO:0000028 denotes bp
T9111 14977-14981 PR:000006847 denotes pH34
T9112 15035-15039 PR:000006847 denotes pH34
T9113 15089-15096 SO:0000112 denotes primers
T9114 15102-15106 SO:0000243 denotes IRES
T9115 15116-15120 SO:0000243 denotes IRES
T9116 15126-15135 SO:0005853 denotes cassettes
T9117 15141-15148 GO:0006266 denotes ligated
T9118 15198-15203 CHEBI:27026 denotes toxin
T9119 15206-15214 SO:0005853 denotes cassette
T9120 15290-15307 SO:0001644 denotes targeting vectors
T9121 15347-15355 CL:0002322 denotes ES cells
T9122 15369-15373 SO:0000704 denotes Gene
T9123 15391-15402 GO:0009294 denotes Transfected
T9124 15438-15442 CHEBI:42768 denotes G418
T9125 15456-15468 CHEBI:16976 denotes hygromycin B
T9126 15484-15491 SO:0001026 denotes Genomic
T9127 15501-15505 CHEBI:42768 denotes G418
T9128 15510-15522 CHEBI:16976 denotes hygromycin B
T9778 15660-15668 CL:0002322 denotes ES cells
T9779 15669-15676 SO:0001026 denotes genomic
T9780 15710-15720 GO:0019835 denotes Cell Lysis
T9781 15721-15729 CHEBI:75958 denotes Solution
T9782 15779-15786 SO:0001026 denotes genomic
T9783 15847-15854 CHEBI:2511 denotes agarose
T9784 15880-15885 CHEBI:25614 denotes nylon
T9785 15920-15922 SO:0000028 denotes bp
T9786 16042-16049 SO:0000112 denotes primers
T9787 16089-16091 SO:0000028 denotes bp
T9788 16129-16131 SO:0000028 denotes bp
T9789 16169-16171 SO:0000028 denotes bp
T9790 16198-16205 SO:0001026 denotes Genomic
T9791 16277-16279 SO:0000028 denotes bp
T9792 16407-16414 SO:0000112 denotes primers
T9793 16426-16436 GO:0097617 denotes hybridized
T9794 16449-16451 SO:0000028 denotes bp
T9795 16491-16493 SO:0000028 denotes bp
T9796 16532-16534 SO:0000028 denotes bp
T10358 16581-16585 PR:000006847 denotes ESG1
T10359 16597-16607 GO:0042571 denotes antibodies
T10360 16631-16635 PR:000006847 denotes Esg1
T10361 16666-16670 PR:000006847 denotes pH34
T10362 16723-16727 PR:000006847 denotes pH34
T10363 16776-16783 SO:0000112 denotes primers
T10364 16804-16808 PR:000006847 denotes pH34
T10365 16824-16835 SO:0000006 denotes PCR product
T10366 16884-16888 PR:000006847 denotes pH34
T10367 16987-16997 GO:0010467 denotes expression
T10368 16998-17004 SO:0000440 denotes vector
T10369 17013-17017 PR:000006847 denotes pH34
T10370 17031-17038 NCBITaxon:562 denotes E. coli
T10371 17065-17083 GO:0006412 denotes protein production
T10372 17154-17158 PR:000006847 denotes ESG1
T10373 17181-17202 CHEBI:44557 denotes nitrilotriacetic acid
T10374 17203-17210 CHEBI:2511 denotes agarose
T10375 17271-17275 CHEBI:16199 denotes urea
T10376 17304-17308 CHEBI:16199 denotes urea
T10377 17372-17379 NCBITaxon:9986 denotes rabbits
T10378 17397-17401 PR:000006847 denotes ESG1
T10379 17413-17423 GO:0042571 denotes antibodies
T10816 17460-17467 CL:0002322 denotes ES cell
T10817 17534-17556 CHEBI:8984 denotes sodium dodecyl sulfate
T10818 17558-17561 CHEBI:8984 denotes SDS
T10819 17606-17620 CHEBI:53325 denotes nitrocellulose
T10820 17679-17683 PR:000006847 denotes ESG1
T10821 17707-17711 PR:000013044 denotes Oct3
T10822 17754-17758 PR:000005257 denotes CDK4
T10823 17829-17839 GO:0042571 denotes antibodies
T10824 17841-17852 NCBITaxon:3704 denotes Horseradish
T10825 17864-17874 MOP:0000779 denotes conjugated
T10826 17880-17886 NCBITaxon:9986 denotes rabbit
T10827 17896-17901 NCBITaxon:10088 denotes mouse
T10828 17902-17917 GO:0019814 denotes immunoglobulins
T10829 17963-17971 GO:0042571 denotes antibody
T10830 18001-18009 GO:0042571 denotes antibody
T11412 18083-18087 PR:000006847 denotes ESG1
T11413 18098-18106 CL:0002322 denotes ES cells
T11414 18112-18123 UBERON:0000358 denotes blastocysts
T11415 18124-18128 PR:000006847 denotes Esg1
T11416 18134-18138 PR:000006847 denotes ESG1
T11417 18156-18160 NCBITaxon:10088 denotes mice
T11418 18180-18189 CHEBI:41774 denotes Tamoxifen
T11419 18202-18214 CHEBI:6716 denotes Depo-provera
T11420 18257-18266 GO:0007565 denotes pregnancy
T11421 18285-18292 UBERON:0000922 denotes embryos
T11422 18329-18335 UBERON:0000995 denotes uterus
T11423 18460-18466 NCBITaxon:27592 denotes Bovine
T11424 18467-18472 UBERON:0001977 denotes Serum
T11425 18570-18580 CHEBI:17334 denotes Penicillin
T11426 18581-18593 CHEBI:17076 denotes Streptomysin
T11427 18620-18637 CHEBI:41218 denotes 2-mercaptoethanol
T11428 18755-18762 PR:000027795 denotes trypsin
T11429 18907-18914 PR:000027795 denotes trypsin
T11430 19013-19020 _FRAGMENT denotes primary
T11431 19024-19028 CL:0000001 denotes cell
T11432 19021-19028 CL:0002322 denotes ES cell
T11433 19118-19129 UBERON:0000119 denotes cell layers
T11858 19242-19250 CL:0002322 denotes ES cells
T11859 19255-19259 PR:000006847 denotes ESG1
T11860 19263-19271 CL:0002322 denotes ES cells
T11861 19289-19292 CHEBI:37987 denotes Cy3
T11862 19297-19300 CHEBI:37989 denotes Cy5
T11863 19333-19343 GO:0097617 denotes hybridized
T11864 19349-19354 NCBITaxon:10088 denotes Mouse
T11865 19503-19516 GO:0097617 denotes Hybridization
T12038 3250-3254 PR:000006847 denotes ESG1
T12039 3254-3265 SO:0000336 denotes pseudogenes
T12040 3298-3303 NCBITaxon:10088 denotes mouse
T12041 3304-3311 SO:0001026 denotes genomic
T12042 3376-3382 SO:0000188 denotes Intron
T12043 3388-3391 SO:0000730 denotes gap
T12236 4927-4930 SO:0000153 denotes BAC
T12237 4953-4957 PR:000006847 denotes ESG1
T12238 4958-4962 SO:0000704 denotes gene
T12239 4981-4991 SO:0000336 denotes pseudogene
T12240 4993-4995 SO:0000336 denotes PS
T12241 5021-5025 SO:0000704 denotes gene
T12242 5030-5032 SO:0000336 denotes PS
T12243 5080-5084 SO:0000704 denotes gene
T12244 5089-5091 SO:0000336 denotes PS
T12245 5116-5126 SO:0000857 denotes homologous
T12246 5138-5148 SO:0000028 denotes base pairs
T12247 5171-5175 SO:0000147 denotes exon
T12248 5183-5185 SO:0000028 denotes bp
T12249 5210-5214 SO:0000147 denotes exon
T12508 5327-5335 SO:0000167 denotes Promoter
T12509 5336-5344 SO:0000165 denotes enhancer
T12510 5361-5365 PR:000006847 denotes ESG1
T12511 5366-5370 SO:0000704 denotes gene
T12512 5375-5385 SO:0000336 denotes pseudogene
T12513 5408-5410 SO:0000028 denotes bp
T12514 5429-5448 SO:0001416 denotes 5' flanking regions
T12515 5456-5460 SO:0000704 denotes gene
T12516 5465-5467 SO:0000336 denotes PS
T12517 5510-5518 SO:0000155 denotes plasmids
T12518 5547-5552 SO:0000704 denotes genes
T12519 5575-5583 CL:0002322 denotes ES cells
T12520 5600-5613 CHEBI:26536 denotes retinoic acid
T12521 5622-5630 CL:0002322 denotes ES cells
T13011 7577-7582 NCBITaxon:10088 denotes mouse
T13012 7583-7587 PR:000006847 denotes ESG1
T13013 7588-7592 SO:0000704 denotes gene
T13014 7666-7683 SO:0000409 denotes Recognition sites
T13015 7769-7773 SO:0000704 denotes gene
T13016 7794-7799 CHEBI:27026 denotes toxin
T13017 7842-7859 SO:0001644 denotes targeting vectors
T13018 7971-7979 CL:0002322 denotes ES cells
T13019 7994-8002 CL:0002322 denotes ES cells
T13020 8016-8024 CL:0002322 denotes ES cells
T13021 8031-8035 PR:000006847 denotes ESG1
T13022 8041-8049 CL:0002322 denotes ES cells
T13023 8155-8163 CL:0002322 denotes ES cells
T13024 8170-8174 PR:000006847 denotes ESG1
T13025 8180-8188 CL:0002322 denotes ES cells
T13026 8226-8234 CL:0002322 denotes ES cells
T13027 8348-8364 CHEBI:4883 denotes ethidium bromide
T13028 8377-8386 GO:0005840 denotes ribosomal
T13541 9480-9484 PR:000006847 denotes ESG1
T13542 9490-9498 CL:0002322 denotes ES cells
T13543 9521-9525 PR:000006847 denotes ESG1
T13544 9531-9538 CL:0002322 denotes ES cell
T13545 9548-9553 GO:0040007 denotes grown
T13546 9610-9614 PR:000006847 denotes ESG1
T13547 9649-9657 CL:0002322 denotes ES cells
T13548 9933-9937 PR:000006847 denotes ESG1
T13549 9943-9951 CL:0002322 denotes ES cells
T13550 9953-9964 CHEBI:51686 denotes hematoxylin
T13911 10875-10879 SO:0000704 denotes Gene
T13912 10875-10890 GO:0010467 denotes Gene expression
T13913 10903-10907 PR:000006847 denotes ESG1
T13914 10913-10921 CL:0002322 denotes ES cells
T13915 10976-10984 CL:0002322 denotes ES cells
T13916 10989-10993 PR:000006847 denotes ESG1
T13917 10999-11007 CL:0002322 denotes ES cells
T13918 11026-11029 CHEBI:37987 denotes Cy3
T13919 11034-11037 CHEBI:37989 denotes Cy5
T13920 11066-11076 GO:0097617 denotes hybridized
T13921 11088-11093 NCBITaxon:10088 denotes Mouse
T13922 11215-11219 PR:000006847 denotes ESG1
T13923 11227-11231 PR:000006847 denotes ESG1
T13924 11259-11269 GO:0010467 denotes expression
T13925 11273-11277 PR:000006847 denotes ESG1
T13926 11279-11283 PR:000013044 denotes Oct3
T13927 11290-11294 PR:000005257 denotes CDK4
T2044 1328-1337 GO:0010467 denotes expressed
T2045 1370-1378 CL:0002322 denotes ES cells
T2046 1380-1389 UBERON:0000922 denotes embryonic
T2047 1380-1389 _FRAGMENT denotes embryonic
T2048 1395-1400 CL:0002321 denotes cells
T2049 1390-1400 CL:0000586 denotes germ cells
T3839 4587-4605 SO:0001416 denotes 5' flanking region
T3840 4644-4652 SO:0000167 denotes promoter
T3841 4653-4661 SO:0000165 denotes enhancer
T3842 4721-4729 CL:0002322 denotes ES cells
T3843 4742-4755 CL:0002371 denotes somatic cells
T3844 4852-4865 CHEBI:26536 denotes retinoic acid
T3845 4906-4910 PR:000006847 denotes ESG1
T3846 4911-4915 SO:0000704 denotes gene
T3847 5795-5798 SO:0000153 denotes BAC
T3848 5824-5828 PR:000006847 denotes ESG1
T3849 5894-5896 SO:0000028 denotes bp
T3850 5897-5916 SO:0000605 denotes intergenic sequence
T3851 5960-5964 PR:000006847 denotes ESG1
T3852 6022-6027 SO:0000147 denotes exons
T3853 6032-6039 SO:0000188 denotes introns
T3854 6047-6051 PR:000006847 denotes ESG1
T3855 6052-6056 SO:0000704 denotes gene
T3856 6140-6145 SO:0000147 denotes exons
T3857 6178-6182 SO:0000147 denotes exon
T3858 6211-6221 SO:0000028 denotes base pairs
T3859 6229-6248 SO:0001417 denotes 3' flanking regions
T3860 6276-6280 PR:000006847 denotes ESG1
T3861 6281-6285 SO:0000704 denotes gene
T3862 6294-6304 SO:0000336 denotes pseudogene
T3863 6316-6326 SO:0000028 denotes base pairs
T3864 6334-6352 SO:0001416 denotes 5' flanking region
T3865 6374-6392 SO:0001416 denotes 5' flanking region
T3866 6398-6400 SO:0000028 denotes bp
T3867 6422-6430 SO:0000167 denotes promoter
T3868 6431-6439 SO:0000165 denotes enhancer
T3869 6548-6558 GO:0006412 denotes translated
T3870 6632-6642 SO:0000336 denotes pseudogene
T3871 6694-6705 SO:0000336 denotes pseudogenes
T3872 6719-6723 SO:0000147 denotes exon
T3873 6724-6730 SO:0000188 denotes intron
T3874 6751-6755 PR:000006847 denotes ESG1
T3875 6756-6760 SO:0000704 denotes gene
T6141 8443-8451 CL:0002322 denotes ES cells
T6142 8477-8483 SO:0000440 denotes vector
T6143 8507-8515 CL:0002322 denotes ES cells
T6144 8524-8528 CHEBI:42768 denotes G418
T6145 8579-8583 PR:000006847 denotes ESG1
T6146 8659-8663 PR:000006847 denotes ESG1
T6147 8716-8722 SO:0000440 denotes vector
T6148 8745-8751 SO:0000440 denotes vector
T6149 8834-8840 SO:0000440 denotes vector
T6150 8881-8885 PR:000006847 denotes ESG1
T6151 8889-8897 CL:0002322 denotes ES cells
T6152 8956-8968 GO:0008283 denotes proliferated
T6153 9062-9066 PR:000006847 denotes ESG1
T6154 9121-9147 PR:000009799 denotes leukemia inhibitory factor
T6155 9163-9176 CHEBI:26536 denotes retinoic acid
T6156 9228-9234 UBERON:0000180 denotes flanks
T6157 9243-9247 NCBITaxon:10088 denotes mice
T6158 9323-9334 UBERON:0000923 denotes germ layers
T6159 9376-9380 PR:000006847 denotes ESG1
T6160 9404-9416 GO:0017145 denotes self-renewal
T6161 9448-9456 CL:0002322 denotes ES cells
T6162 9987-9988 CL:0002322 denotes e
T6163 9988-9989 CHEBI:51686 denotes x
T6164 10000-10004 SO:0000704 denotes gene
T6165 10000-10015 GO:0010467 denotes gene expression
T6166 10028-10032 PR:000006847 denotes ESG1
T6167 10036-10044 CL:0002322 denotes ES cells
T6168 10110-10115 SO:0000704 denotes genes
T6169 10142-10150 CL:0002322 denotes ES cells
T6170 10152-10156 PR:000006847 denotes ESG1
R3166 T11431 T11430 _lexicallyChainedTo cell,primary
R313 T2003 T2002 _lexicallyChainedTo cells,Embryonic stem
R314 T2003 T2004 _lexicallyChainedTo cells,ES
R315 T2017 T2016 _lexicallyChainedTo cells,proliferation of
R316 T2038 T2037 _lexicallyChainedTo cells,embryonic
R317 T2048 T2047 _lexicallyChainedTo cells,embryonic
R318 T2051 T2050 _lexicallyChainedTo stem cells,multipotent

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T2085 11-20 UBERON:0000922 denotes Embryonic
T2086 11-25 _FRAGMENT denotes Embryonic stem
T2087 31-36 CL:0002322 denotes cells
T2088 27-29 _FRAGMENT denotes ES
T2089 31-36 CL_GO_EXT:cell denotes cells
T2090 65-76 UBERON:0000358 denotes blastocysts
T2091 80-84 NCBITaxon:10088 denotes mice
T2092 103-109 NCBITaxon:9606 denotes humans
T2093 123-131 CL:0002322 denotes ES cells
T2094 126-131 CL_GO_EXT:cell denotes cells
T2095 224-237 GO_RO_EXT:developmental_differentiation_process denotes differentiate
T2096 261-266 CL_GO_EXT:cell denotes cells
T2097 278-291 GO:0008283 denotes proliferation
T2098 320-325 NCBITaxon:10088 denotes mouse
T2099 326-334 CL:0002322 denotes ES cells
T2100 329-334 CL_GO_EXT:cell denotes cells
T2101 361-365 SO_EXT:0000704 denotes gene
T2102 413-418 NCBITaxon:9606 denotes human
T2103 419-427 CL:0002322 denotes ES cells
T2104 422-427 CL_GO_EXT:cell denotes cells
T2105 451-455 CL_GO_EXT:cell denotes cell
T2106 517-528 UBERON:0002240 denotes spinal cord
T2107 542-550 PATO_UBERON_EXT:juvenile_organism denotes juvenile
T2108 565-574 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T2109 624-640 _FRAGMENT denotes proliferation of
T2110 644-649 GO:0008283 denotes cells
T2111 641-649 CL:0002322 denotes ES cells
T2112 644-649 CL_GO_EXT:cell denotes cells
T2113 694-703 CL:0000034 denotes stem cell
T2114 699-703 CL_GO_EXT:cell denotes cell
T2115 704-711 CHEBI_GO_EXT:biological_process_or_quality_or_role denotes biology
T2116 731-740 CHEBI:36357 denotes molecules
T2117 754-762 CL:0002322 denotes ES cells
T2118 757-762 CL_GO_EXT:cell denotes cells
T2119 849-854 SO_EXT:0000704 denotes genes
T2120 868-877 GO:0010467 denotes expressed
T2121 881-889 CL:0002322 denotes ES cells
T2122 884-889 CL_GO_EXT:cell denotes cells
T2123 894-907 UBERON:0019248 denotes early embryos
T2124 935-938 CHEBI_SO_EXT:DNA denotes DNA
T2125 967-976 GO:0010467 denotes expressed
T2126 967-989 SO_EXT:0000345 denotes expressed sequence tag
T2127 986-989 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes tag
T2128 1033-1044 SO_EXT:0000673 denotes transcripts
T2129 1056-1060 PR_EXT:000006847 denotes ESG1
T2130 1086-1091 PR_EXT:000006847 denotes dppa5
T2131 1095-1100 PR_EXT:000006847 denotes ECAT2
T2132 1102-1106 PR_EXT:000006847 denotes ESG1
T2133 1138-1148 SO_EXT:0000673 denotes transcript
T2134 1149-1153 PR_EXT:000006847 denotes Ph34
T2135 1163-1177 GO_EXT:negative_regulation denotes down-regulated
T2136 1181-1194 CHEBI:26536 denotes retinoic acid
T2137 1198-1207 UBERON:0000922 denotes embryonic
T2138 1198-1207 _FRAGMENT denotes embryonic
T2139 1218-1223 CL:0002321 denotes cells
T2140 1218-1223 CL_GO_EXT:cell denotes cells
T2141 1234-1244 GO:0010467 denotes expression
T2142 1253-1257 SO_EXT:0000704 denotes gene
T2143 1274-1278 NCBITaxon:10088 denotes mice
T2144 1282-1295 UBERON:0019248 denotes early embryos
T2145 1300-1310 CL:0000586 denotes germ cells
T2146 1305-1310 CL_GO_EXT:cell denotes cells
T2147 1328-1337 GO:0010467 denotes expressed
T2148 1353-1358 CL_GO_EXT:cell denotes cells
T2149 1370-1378 CL:0002322 denotes ES cells
T2150 1373-1378 CL_GO_EXT:cell denotes cells
T2151 1380-1389 UBERON:0000922 denotes embryonic
T2152 1380-1389 _FRAGMENT denotes embryonic
T2153 1395-1400 CL:0002321 denotes cells
T2154 1390-1400 CL:0000586 denotes germ cells
T2155 1395-1400 CL_GO_EXT:cell denotes cells
T2156 1406-1417 _FRAGMENT denotes multipotent
T2157 1427-1437 CL:0000048 denotes stem cells
T2158 1418-1437 CL:0000014 denotes germline stem cells
T2159 1432-1437 CL_GO_EXT:cell denotes cells
T2160 1444-1448 PR_EXT:000006847 denotes ESG1
T2161 1449-1456 SO_EXT:sequence_coding_function denotes encodes
T2162 1459-1470 CHEBI_SO_EXT:polypeptide denotes polypeptide
T2163 1478-1489 CHEBI_SO_EXT:amino_acid denotes amino acids
T2164 1516-1522 SO_EXT:0000417 denotes domain
T2165 1536-1539 CHEBI_SO_EXT:RNA denotes RNA
T2166 1540-1547 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T2167 1548-1554 SO_EXT:0000417 denotes domain
T2168 1593-1597 PR_EXT:000006847 denotes ESG1
T2169 1614-1617 CHEBI_SO_EXT:RNA denotes RNA
T2170 1618-1625 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T2171 1626-1633 CHEBI_PR_EXT:protein denotes protein
T2172 1659-1667 CL:0002322 denotes ES cells
T2173 1662-1667 CL_GO_EXT:cell denotes cells
T2174 1672-1676 NCBITaxon:10088 denotes mice
T2175 1687-1694 SO_EXT:0001026 denotes genomic
T2176 1727-1741 SO_EXT:0000040 denotes genomic clones
T2177 1757-1762 NCBITaxon:10088 denotes mouse
T2178 1763-1767 PR_EXT:000006847 denotes ESG1
T2179 1768-1772 SO_EXT:0000704 denotes gene
T2180 1783-1794 SO_EXT:0000336 denotes pseudogenes
T2181 1814-1825 SO_EXT:0000336 denotes pseudogenes
T2182 1844-1848 SO_EXT:0000147 denotes exon
T2183 1849-1855 SO_EXT:0000188 denotes intron
T2184 1873-1877 PR_EXT:000006847 denotes ESG1
T2185 1878-1882 SO_EXT:0000704 denotes gene
T2186 1915-1919 SO_EXT:0000704 denotes gene
T2187 1920-1931 SO_EXT:sequence_duplication_entity_or_process denotes duplication
T2188 1952-1963 SO_EXT:0000336 denotes pseudogenes
T2189 1984-1991 SO_EXT:0000188 denotes introns
T2190 2033-2051 SO_EXT:sequence_retrotransposition_entity_or_process denotes retrotransposition
T2191 2033-2051 GO:0032197 denotes retrotransposition
T2192 2055-2059 PR_EXT:000006847 denotes ESG1
T2193 2060-2071 SO_EXT:0000673 denotes transcripts
T2194 2077-2088 GO_SO_EXT:chromosome denotes chromosomal
T2195 2089-2102 GO_PATO_EXT:biological_localization_process_or_quality denotes localizations
T2196 2110-2115 NCBITaxon:10088 denotes mouse
T2197 2116-2120 PR_EXT:000006847 denotes ESG1
T2198 2121-2125 SO_EXT:0000704 denotes gene
T2199 2130-2141 SO_EXT:0000336 denotes pseudogenes
T2200 2226-2231 NCBITaxon:10088 denotes mouse
T2201 2232-2236 SO_EXT:0000704 denotes gene
T2202 2237-2245 SO_EXT:sequence_coding_function denotes encoding
T2203 2251-2258 CHEBI_PR_EXT:protein denotes protein
T2204 2271-2282 SO_EXT:0000336 denotes pseudogenes
T2205 2302-2306 SO_EXT:0000704 denotes gene
T2206 2334-2347 GO_PATO_EXT:physiological_process_or_quality denotes physiological
T2207 2360-2364 PR_EXT:000006847 denotes ESG1
T3878 2391-2402 GO_SO_EXT:chromosome denotes Chromosomal
T3879 2403-2415 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T3880 2434-2439 NCBITaxon:10088 denotes mouse
T3881 2440-2444 PR_EXT:000006847 denotes ESG1
T3894 2627-2635 SO_EXT:biological_sequence denotes sequence
T3895 2679-2690 SO_EXT:0000336 denotes pseudogenes
T3896 2699-2706 SO_EXT:0000188 denotes introns
T3897 2710-2721 GO_SO_EXT:chromosome denotes chromosomes
T3898 2783-2789 SO_EXT:0000188 denotes intron
T3899 2795-2806 SO_EXT:0000336 denotes pseudogenes
T3900 2826-2829 CHEBI_SO_EXT:DNA denotes DNA
T3901 2854-2865 GO_SO_EXT:chromosome denotes chromosomal
T3902 2866-2878 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T3903 2910-2922 SO_EXT:0000336 denotes pseudodgenes
T3904 2940-2948 SO:0000857 denotes homology
T3905 2952-2956 PR_EXT:000006847 denotes ESG1
T3906 2957-2961 SO_EXT:cDNA denotes cDNA
T3907 2997-3005 CHEBI_PR_EXT:protein denotes proteins
T3908 3027-3036 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T3909 3093-3099 SO_EXT:0000188 denotes intron
T3910 3105-3116 SO_EXT:0000336 denotes pseudogenes
T3911 3168-3184 SO_EXT:0000043 denotes retropseudogenes
T3912 3214-3218 CL_GO_EXT:cell denotes cell
T3913 3228-3233 SO_EXT:0000704 denotes genes
T3914 3485-3495 GO_SO_EXT:chromosome denotes chromosome
T3915 3515-3518 CHEBI_SO_EXT:DNA denotes DNA
T3916 3543-3547 PR_EXT:000006847 denotes ESG1
T3917 3548-3552 SO_EXT:0000704 denotes gene
T3918 3580-3587 SO_EXT:0000188 denotes introns
T3919 3621-3626 SO_EXT:0000147 denotes exons
T3920 3660-3669 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T3921 3677-3681 PR_EXT:000006847 denotes ESG1
T3922 3682-3686 SO_EXT:cDNA denotes cDNA
T3923 3687-3695 SO_EXT:biological_sequence denotes sequence
T3924 3716-3720 SO_EXT:0000147 denotes exon
T3925 3770-3774 PR_EXT:000006847 denotes ESG1
T3926 3775-3779 SO_EXT:0000704 denotes gene
T3927 3789-3799 GO_SO_EXT:chromosome denotes chromosome
T3928 3825-3828 CHEBI_SO_EXT:DNA denotes DNA
T3929 3888-3892 SO_EXT:0000147 denotes exon
T3930 3900-3904 PR_EXT:000006847 denotes ESG1
T3931 3905-3909 SO_EXT:0000704 denotes gene
T3932 3945-3949 PR_EXT:000006847 denotes ESG1
T3933 3955-3964 SO_EXT:biological_sequence denotes sequences
T3934 3968-3978 GO_SO_EXT:chromosome denotes chromosome
T3935 4026-4029 CHEBI_SO_EXT:DNA denotes DNA
T3936 4055-4059 PR_EXT:000006847 denotes ESG1
T3937 4060-4064 SO_EXT:0000704 denotes gene
T3938 4082-4091 NCBITaxon:2 denotes bacterial
T3939 4082-4113 SO_EXT:0000153 denotes bacterial artificial chromosome
T3940 4103-4113 GO_SO_EXT:chromosome denotes chromosome
T3941 4115-4118 SO_EXT:0000153 denotes BAC
T3942 4120-4123 CHEBI_SO_EXT:DNA denotes DNA
T3943 4142-4148 SO_EXT:0000112 denotes primer
T3944 4188-4192 SO_EXT:0000704 denotes gene
T3945 4202-4213 SO_EXT:0000336 denotes pseudogenes
T3946 4260-4263 SO_EXT:0000153 denotes BAC
T3947 4264-4270 SO_EXT:sequence_cloned_entity denotes clones
T3948 4334-4340 SO_EXT:sequence_cloned_entity denotes clones
T3949 4353-4361 SO_EXT:biological_sequence denotes sequence
T3950 4396-4400 PR_EXT:000006847 denotes ESG1
T3951 4401-4405 SO_EXT:cDNA denotes cDNA
T3952 4443-4450 SO_EXT:0000188 denotes introns
T3953 4472-4478 SO_EXT:0000188 denotes intron
T3954 4479-4488 SO_EXT:biological_sequence denotes sequences
T4017 6756-6760 SO_EXT:0000704 denotes gene
T4018 6809-6820 SO_EXT:0000336 denotes pseudogenes
T4019 6891-6901 SO_EXT:0000336 denotes pseudogene
T6232 6931-6936 NCBITaxon:10088 denotes mouse
T6233 6937-6941 PR_EXT:000006847 denotes ESG1
T6234 6942-6946 SO_EXT:0000704 denotes gene
T6235 6972-6976 PR_EXT:000006847 denotes ESG1
T6236 6981-6988 SO_EXT:sequence_deletion_process denotes deleted
T6237 6993-6997 SO_EXT:0000704 denotes gene
T6238 7012-7025 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T6239 7029-7034 NCBITaxon:10088 denotes mouse
T6240 7035-7043 CL:0002322 denotes ES cells
T6241 7038-7043 CL_GO_EXT:cell denotes cells
T6242 7048-7056 SO_EXT:sequence_substitution_process denotes replaced
T6243 7067-7072 SO_EXT:0000147 denotes exons
T6244 7087-7096 _FRAGMENT denotes fusion of
T6245 7141-7146 SO_EXT:fusion_gene_or_gene_fusion denotes genes
T6246 7101-7109 CHEBI:7507 denotes neomycin
T6247 7125-7140 GO_EXT:0004565 denotes β-galactosidase
T6248 7148-7153 GO_EXT:beta_geo denotes β-geo
T6249 7162-7172 CHEBI:24753 denotes hygromycin
T6250 7183-7187 SO_EXT:0000704 denotes gene
T6251 7205-7222 SO_EXT:0001644 denotes targeting vectors
T6252 7255-7263 CL:0002322 denotes ES cells
T6253 7258-7263 CL_GO_EXT:cell denotes cells
T6254 7302-7309 CL:0002322 denotes ES cell
T6255 7305-7309 CL_GO_EXT:cell denotes cell
T6256 7310-7316 SO_EXT:sequence_cloned_entity denotes clones
T6257 7341-7354 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T6258 7362-7367 GO_EXT:beta_geo denotes β-geo
T6259 7368-7384 SO_EXT:0001644 denotes targeting vector
T6260 7466-7471 SO_EXT:sequence_cloned_entity denotes clone
T6261 7496-7509 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T6262 7522-7538 SO_EXT:0001644 denotes targeting vector
T6301 9255-9260 CL_GO_EXT:cell denotes cells
T6302 9323-9334 UBERON:0000923 denotes germ layers
T6303 9376-9380 PR_EXT:000006847 denotes ESG1
T6304 9404-9416 GO:0017145 denotes self-renewal
T6305 9448-9456 CL:0002322 denotes ES cells
T6306 9451-9456 CL_GO_EXT:cell denotes cells
T6307 9984-9985 SO_EXT:wild_type_entity_or_quality denotes W
T6327 10224-10225 SO_EXT:sequence_nullness_or_absence denotes -
T6328 10226-10234 CL:0002322 denotes ES cells
T6329 10229-10234 CL_GO_EXT:cell denotes cells
T6330 10252-10262 GO:0010467 denotes expression
T6331 10266-10273 CL:0002322 denotes ES cell
T6332 10269-10273 CL_GO_EXT:cell denotes cell
T6333 10274-10280 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T6334 10281-10286 SO_EXT:0000704 denotes genes
T6335 10296-10301 PR_EXT:000010968 denotes Nanog
T6336 10306-10310 PR_EXT:000013044 denotes Oct3
T6337 10328-10332 PR_EXT:000006847 denotes ESG1
T6338 10332-10333 SO_EXT:sequence_nullness_or_absence denotes -
T6339 10334-10335 SO_EXT:sequence_nullness_or_absence denotes -
T6340 10336-10344 CL:0002322 denotes ES cells
T6341 10339-10344 CL_GO_EXT:cell denotes cells
T6342 10366-10370 PR_EXT:000013044 denotes Oct3
T6343 10373-10383 GO:0010467 denotes expression
T6344 10387-10394 CHEBI_PR_EXT:protein denotes protein
T6345 10443-10447 SO_EXT:0000704 denotes gene
T6346 10443-10458 GO:0010467 denotes gene expression
T6347 10497-10505 CL:0002322 denotes ES cells
T6348 10500-10505 CL_GO_EXT:cell denotes cells
T6349 10510-10514 PR_EXT:000006847 denotes ESG1
T6350 10514-10515 SO_EXT:sequence_nullness_or_absence denotes -
T6351 10516-10517 SO_EXT:sequence_nullness_or_absence denotes -
T6352 10518-10526 CL:0002322 denotes ES cells
T6353 10521-10526 CL_GO_EXT:cell denotes cells
T6354 10536-10541 SO_EXT:0000704 denotes genes
T6355 10589-10593 PR_EXT:000006847 denotes ESG1
T6356 10593-10594 SO_EXT:sequence_nullness_or_absence denotes -
T6357 10595-10596 SO_EXT:sequence_nullness_or_absence denotes -
T6358 10597-10602 CL_GO_EXT:cell denotes cells
T6359 10614-10618 PR_EXT:000009450 denotes Krt1
T6360 10622-10625 PR_EXT:000014397 denotes Pem
T6361 10627-10631 PR_EXT:000003172 denotes Ctgf
T6362 10633-10638 PR_EXT:000013428 denotes Ptgs2
T6363 10640-10644 CHEBI_PR_EXT:insulin_like_growth_factor_2 denotes Igf2
T6364 10649-10654 PR_EXT:000000216 denotes Inhba
T6365 10662-10667 SO_EXT:0000704 denotes genes
T6366 10677-10686 GO:0065007 denotes regulated
T6367 10713-10717 PR_EXT:000006847 denotes ESG1
T6368 10725-10729 PR_EXT:000006847 denotes ESG1
T6369 10749-10752 CHEBI_SO_EXT:RNA denotes RNA
T6370 10753-10760 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T6371 10761-10767 SO_EXT:0000417 denotes domain
T6372 10786-10790 CHEBI_SO_EXT:mRNA denotes mRNA
T6373 10800-10805 SO_EXT:0000704 denotes genes
T6401 11598-11599 SO_EXT:sequence_nullness_or_absence denotes -
T6403 11612-11619 NCBITaxon:33208 denotes animals
T6404 11723-11729 UBERON:0000473 denotes testis
T6405 11734-11739 UBERON:0000992 denotes ovary
T6406 11819-11823 PR_EXT:000006847 denotes ESG1
T6407 11848-11853 NCBITaxon:10088 denotes mouse
T6408 11870-11879 CL:0000586 denotes germ cell
T6409 11870-11889 GO:0007281 denotes germ cell formation
T6410 11875-11879 CL_GO_EXT:cell denotes cell
T6411 11909-11917 CL:0002322 denotes ES cells
T6412 11912-11917 CL_GO_EXT:cell denotes cells
T6413 11923-11934 UBERON:0000358 denotes blastocysts
T6414 11963-11967 PR_EXT:000006847 denotes ESG1
T6415 11967-11968 SO_EXT:normal_or_wild_type_or_present denotes +
T6416 11969-11970 SO_EXT:sequence_nullness_or_absence denotes -
T6417 11971-11976 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes males
T6418 11981-11985 PR_EXT:000006847 denotes ESG1
T6419 11985-11986 SO_EXT:sequence_nullness_or_absence denotes -
T6420 11987-11988 SO_EXT:sequence_nullness_or_absence denotes -
T6421 11989-11996 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T6422 12011-12018 CL:0002322 denotes ES cell
T6423 12014-12018 CL_GO_EXT:cell denotes cell
T6424 12042-12048 SO_EXT:sequence_cloned_entity denotes clones
T6425 12054-12058 PR_EXT:000006847 denotes ESG1
T6426 12058-12059 SO_EXT:sequence_nullness_or_absence denotes -
T6427 12060-12061 SO_EXT:sequence_nullness_or_absence denotes -
T6428 12069-12073 PR_EXT:000006847 denotes ESG1
T6429 12074-12078 SO_EXT:sequence_nullness denotes null
T6430 12079-12087 CL:0002322 denotes ES cells
T6431 12082-12087 CL_GO_EXT:cell denotes cells
T6432 12120-12133 GO:0008283 denotes proliferation
T6433 12139-12154 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T6434 12184-12188 PR_EXT:000006847 denotes ESG1
T6435 12207-12215 CL:0002322 denotes ES cells
T6436 12210-12215 CL_GO_EXT:cell denotes cells
T7347 12267-12271 PR_EXT:000006847 denotes ESG1
T7348 12244-12257 GO_PATO_EXT:physiological_process_or_quality denotes physiological
T7349 12291-12296 NCBITaxon:10088 denotes mouse
T7350 12297-12301 SO_EXT:0000704 denotes gene
T7351 12305-12315 GO_SO_EXT:chromosome denotes chromosome
T7352 12322-12329 SO_EXT:sequence_deletion_process denotes deleted
T7353 12347-12360 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T7354 12364-12372 CL:0002322 denotes ES cells
T7355 12367-12372 CL_GO_EXT:cell denotes cells
T7356 12391-12401 GO:0010467 denotes expression
T7357 12405-12418 UBERON:0019248 denotes early embryos
T7358 12420-12430 CL:0000586 denotes germ cells
T7359 12425-12430 CL_GO_EXT:cell denotes cells
T7360 12448-12453 CL_GO_EXT:cell denotes cells
T7361 12482-12486 PR_EXT:000006847 denotes ESG1
T7362 12506-12511 NCBITaxon:10088 denotes mouse
T7363 12525-12534 CL:0000586 denotes germ cell
T7364 12525-12544 GO:0007281 denotes germ cell formation
T7365 12530-12534 CL_GO_EXT:cell denotes cell
T7366 12550-12557 CL:0002322 denotes ES cell
T7367 12553-12557 CL_GO_EXT:cell denotes cell
T7368 12553-12570 GO:0017145 denotes cell self-renewal
T8193 12613-12616 SO_EXT:0000153 denotes BAC
T8194 12617-12623 SO_EXT:sequence_cloned_entity denotes clones
T8195 12639-12644 NCBITaxon:10088 denotes mouse
T8196 12645-12649 PR_EXT:000006847 denotes ESG1
T8197 12650-12654 SO_EXT:0000704 denotes gene
T8198 12667-12676 NCBITaxon:2 denotes bacterial
T8199 12667-12698 SO_EXT:0000153 denotes bacterial artificial chromosome
T8200 12688-12698 GO_SO_EXT:chromosome denotes chromosome
T8201 12700-12703 SO_EXT:0000153 denotes BAC
T8202 12705-12711 SO_EXT:sequence_cloned_entity denotes clones
T8203 12723-12728 NCBITaxon:10088 denotes mouse
T8204 12729-12733 PR_EXT:000006847 denotes ESG1
T8205 12734-12738 SO_EXT:0000704 denotes gene
T8206 12776-12781 NCBITaxon:10088 denotes mouse
T8207 12782-12785 SO_EXT:0000153 denotes BAC
T8208 12794-12797 CHEBI_SO_EXT:DNA denotes DNA
T8209 12814-12822 OBI_SO_EXT:gene_or_genetic_investigation_or_genetic_process denotes Genetics
T8210 12834-12838 PR_EXT:000006847 denotes pH34
T8211 12877-12881 PR_EXT:000006847 denotes pH34
T8212 12916-12923 SO_EXT:0000112 denotes primers
T8213 12935-12953 GO_EXT:0015666 denotes restriction enzyme
T8214 12947-12953 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T8215 13004-13007 SO_EXT:0000153 denotes BAC
T8216 13008-13014 SO_EXT:sequence_cloned_entity denotes clones
T8217 13043-13047 PR_EXT:000006847 denotes pH34
T8218 13093-13097 PR_EXT:000006847 denotes pH34
T8219 13132-13136 PR_EXT:000006847 denotes pH34
T8220 13182-13186 PR_EXT:000006847 denotes pH34
T8221 13229-13233 PR_EXT:000006847 denotes pH34
T8222 13273-13279 CHEBI_SO_EXT:molecular_probe denotes probes
T8223 13281-13294 GO:0097617 denotes Hybridization
T8224 13306-13312 CHEBI_SO_EXT:molecular_probe denotes probes
T8225 13350-13354 PR_EXT:000006847 denotes ESG1
T8226 13355-13359 SO_EXT:0000704 denotes gene
T8227 13363-13374 SO_EXT:0000336 denotes pseudogenes
T8228 13414-13419 NCBITaxon:10088 denotes mouse
T8229 13420-13424 PR_EXT:000006847 denotes ESG1
T8230 13425-13429 SO_EXT:0000704 denotes gene
T8231 13438-13456 SO_EXT:0001417 denotes 3' flanking region
T8232 13478-13480 SO_EXT:0000028 denotes bp
T8233 13517-13523 SO_EXT:0000440 denotes vector
T8234 13538-13545 PR_EXT:P43870 denotes HindIII
T8235 13583-13589 SO_EXT:0000440 denotes vector
T8236 13600-13606 SO_EXT:sequence_cloning_process denotes cloned
T8237 13662-13666 PR_EXT:000006847 denotes ESG1
T8238 13667-13677 SO_EXT:0000336 denotes pseudogene
T8239 13686-13704 SO_EXT:0001417 denotes 3' flanking region
T8240 13712-13714 SO_EXT:0000028 denotes bp
T8241 13738-13744 SO_EXT:sequence_cloning_process denotes cloned
T8242 13769-13774 PR_EXT:P23940 denotes BamHI
T8243 13803-13809 SO_EXT:0000440 denotes vector
T8244 13820-13826 SO_EXT:sequence_cloning_process denotes cloned
T8245 13867-13875 SO_EXT:biological_sequence denotes sequence
T8246 13891-13910 SO_EXT:0001416 denotes 5' flanking regions
T8247 13918-13922 PR_EXT:000006847 denotes ESG1
T8248 13923-13927 SO_EXT:0000704 denotes gene
T8249 13944-13955 SO_EXT:0000336 denotes pseudogenes
T8250 14005-14009 PR_EXT:000006847 denotes pH34
T8251 14057-14061 PR_EXT:000006847 denotes pH34
T8252 14067-14074 SO_EXT:0000112 denotes primers
T8253 14090-14098 SO_EXT:biological_sequence denotes sequence
T8254 14103-14109 SO_EXT:sequence_cloning_process denotes cloned
T8255 14154-14156 SO_EXT:0000028 denotes bp
T8256 14263-14269 SO_EXT:sequence_cloning_process denotes cloned
T8257 14337-14355 SO_EXT:0001416 denotes 5' flanking region
T8258 14363-14367 PR_EXT:000006847 denotes ESG1
T8259 14368-14372 SO_EXT:0000704 denotes gene
T8260 14380-14382 SO_EXT:0000028 denotes bp
T8261 14438-14444 SO_EXT:sequence_cloning_process denotes cloned
T8262 14515-14523 SO:0000357 denotes flanking
T8263 14528-14538 SO_EXT:0000336 denotes pseudogene
T9129 14557-14561 PR_EXT:000006847 denotes ESG1
T9130 14562-14579 SO_EXT:0001644 denotes targeting vectors
T9131 14583-14591 SO_EXT:sequence_substitution_process denotes replaced
T9132 14603-14607 PR_EXT:000006847 denotes ESG1
T9133 14608-14613 SO_EXT:0000147 denotes exons
T9134 14623-14640 SO_EXT:0001644 denotes targeting vectors
T9135 14662-14666 SO_EXT:0000243 denotes IRES
T9136 14667-14672 GO_EXT:beta_geo denotes β-geo
T9137 14673-14681 SO_EXT:0005853 denotes cassette
T9138 14693-14697 SO_EXT:0000243 denotes IRES
T9139 14703-14711 SO_EXT:0005853 denotes cassette
T9140 14715-14723 SO_EXT:0000167 denotes promoter
T9141 14770-14772 SO_EXT:0000028 denotes bp
T9142 14807-14811 PR_EXT:000006847 denotes pH34
T9143 14865-14869 PR_EXT:000006847 denotes pH34
T9144 14923-14930 SO_EXT:0000112 denotes primers
T9145 14949-14951 SO_EXT:0000028 denotes bp
T9146 14977-14981 PR_EXT:000006847 denotes pH34
T9147 15035-15039 PR_EXT:000006847 denotes pH34
T9148 15089-15096 SO_EXT:0000112 denotes primers
T9149 15102-15106 SO_EXT:0000243 denotes IRES
T9150 15107-15112 GO_EXT:beta_geo denotes β-geo
T9151 15116-15120 SO_EXT:0000243 denotes IRES
T9152 15126-15135 SO_EXT:0005853 denotes cassettes
T9153 15141-15148 GO:0006266 denotes ligated
T9154 15198-15203 CHEBI_EXT:27026 denotes toxin
T9155 15206-15214 SO_EXT:0005853 denotes cassette
T9156 15226-15236 SO_EXT:sequence_downstreamness denotes downstream
T9157 15258-15271 SO_EXT:sequence_linearization denotes linearization
T9158 15290-15307 SO_EXT:0001644 denotes targeting vectors
T9159 15347-15355 CL:0002322 denotes ES cells
T9160 15350-15355 CL_GO_EXT:cell denotes cells
T9161 15369-15373 SO_EXT:0000704 denotes Gene
T9162 15391-15402 GO:0009294 denotes Transfected
T9163 15403-15408 CL_GO_EXT:cell denotes cells
T9164 15438-15442 CHEBI:42768 denotes G418
T9165 15456-15468 CHEBI:16976 denotes hygromycin B
T9166 15484-15495 SO_EXT:genomic_DNA denotes Genomic DNA
T9167 15492-15495 CHEBI_SO_EXT:DNA denotes DNA
T9168 15501-15505 CHEBI:42768 denotes G418
T9169 15510-15522 CHEBI:16976 denotes hygromycin B
T9170 15570-15583 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T9797 15646-15659 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T9798 15660-15668 CL:0002322 denotes ES cells
T9799 15663-15668 CL_GO_EXT:cell denotes cells
T9800 15669-15680 SO_EXT:genomic_DNA denotes genomic DNA
T9801 15677-15680 CHEBI_SO_EXT:DNA denotes DNA
T9802 15710-15714 CL_GO_EXT:cell denotes Cell
T9803 15710-15720 GO:0019835 denotes Cell Lysis
T9804 15721-15729 CHEBI:75958 denotes Solution
T9805 15779-15790 SO_EXT:genomic_DNA denotes genomic DNA
T9806 15787-15790 CHEBI_SO_EXT:DNA denotes DNA
T9807 15847-15854 CHEBI:2511 denotes agarose
T9808 15863-15874 GO_EXT:biological_movement_or_translocation_process denotes transferred
T9809 15880-15885 CHEBI:25614 denotes nylon
T9810 15920-15922 SO_EXT:0000028 denotes bp
T9811 15926-15931 CHEBI_SO_EXT:molecular_probe denotes probe
T9812 16042-16049 SO_EXT:0000112 denotes primers
T9813 16055-16060 CHEBI_SO_EXT:molecular_probe denotes probe
T9814 16074-16081 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T9815 16089-16091 SO_EXT:0000028 denotes bp
T9816 16106-16115 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9817 16129-16131 SO_EXT:0000028 denotes bp
T9818 16146-16151 GO_EXT:beta_geo denotes β-geo
T9819 16169-16171 SO_EXT:0000028 denotes bp
T9820 16198-16209 SO_EXT:genomic_DNA denotes Genomic DNA
T9821 16206-16209 CHEBI_SO_EXT:DNA denotes DNA
T9822 16277-16279 SO_EXT:0000028 denotes bp
T9823 16283-16288 CHEBI_SO_EXT:molecular_probe denotes probe
T9824 16407-16414 SO_EXT:0000112 denotes primers
T9825 16420-16425 CHEBI_SO_EXT:molecular_probe denotes probe
T9826 16426-16436 GO:0097617 denotes hybridized
T9827 16449-16451 SO_EXT:0000028 denotes bp
T9828 16466-16475 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9829 16491-16493 SO_EXT:0000028 denotes bp
T9830 16508-16513 GO_EXT:beta_geo denotes β-geo
T9831 16532-16534 SO_EXT:0000028 denotes bp
T10380 16562-16575 _FRAGMENT denotes Generation of
T10381 16597-16607 GO_EXT:antibody_or_immunoglobulin_biosynthesis_or_production denotes antibodies
T10382 16581-16585 PR_EXT:000006847 denotes ESG1
T10383 16597-16607 GO:0042571 denotes antibodies
T10384 16612-16627 SO_EXT:coding_sequence denotes coding sequence
T10385 16631-16635 PR_EXT:000006847 denotes Esg1
T10386 16666-16670 PR_EXT:000006847 denotes pH34
T10387 16723-16727 PR_EXT:000006847 denotes pH34
T10388 16776-16783 SO_EXT:0000112 denotes primers
T10389 16804-16808 PR_EXT:000006847 denotes pH34
T10390 16824-16835 SO_EXT:0000006 denotes PCR product
T10391 16884-16888 PR_EXT:000006847 denotes pH34
T10392 16936-16949 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T10393 16987-16997 GO:0010467 denotes expression
T10394 16998-17004 SO_EXT:0000440 denotes vector
T10395 17013-17017 PR_EXT:000006847 denotes pH34
T10396 17031-17038 NCBITaxon:562 denotes E. coli
T10397 17053-17064 GO_SO_EXT:sequence_rearrangement_process denotes recombinant
T10398 17065-17072 CHEBI_PR_EXT:protein denotes protein
T10399 17065-17083 GO:0006412 denotes protein production
T10400 17137-17146 CHEBI_SO_EXT:histidine denotes Histidine
T10401 17147-17153 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T10402 17154-17158 PR_EXT:000006847 denotes ESG1
T10403 17178-17180 CHEBI_EXT:nickel denotes Ni
T10404 17181-17202 CHEBI:44557 denotes nitrilotriacetic acid
T10405 17203-17210 CHEBI:2511 denotes agarose
T10406 17226-17236 GO_EXT:macromolecule_denaturation denotes denaturing
T10407 17271-17275 CHEBI:16199 denotes urea
T10408 17304-17308 CHEBI:16199 denotes urea
T10409 17314-17325 GO_SO_EXT:sequence_rearrangement_process denotes recombinant
T10410 17326-17334 CHEBI_PR_EXT:protein denotes proteins
T10411 17372-17379 NCBITaxon:9986 denotes rabbits
T10412 17397-17401 PR_EXT:000006847 denotes ESG1
T10413 17413-17423 GO:0042571 denotes antibodies
T10831 17460-17467 CL:0002322 denotes ES cell
T10832 17463-17467 CL_GO_EXT:cell denotes cell
T10833 17498-17506 CL_GO_EXT:cell denotes cellular
T10834 17507-17515 CHEBI_PR_EXT:protein denotes proteins
T10835 17534-17556 CHEBI:8984 denotes sodium dodecyl sulfate
T10836 17558-17561 CHEBI:8984 denotes SDS
T10837 17567-17581 CHEBI_EXT:polyacrylamide denotes polyacrylamide
T10838 17591-17602 GO_EXT:biological_movement_or_translocation_process denotes transferred
T10839 17606-17620 CHEBI:53325 denotes nitrocellulose
T10840 17679-17683 PR_EXT:000006847 denotes ESG1
T10841 17707-17711 PR_EXT:000013044 denotes Oct3
T10842 17754-17758 PR_EXT:000005257 denotes CDK4
T10843 17829-17839 GO:0042571 denotes antibodies
T10844 17841-17852 NCBITaxon:3704 denotes Horseradish
T10845 17853-17863 GO_EXT:0004601 denotes peroxidase
T10846 17864-17874 MOP:0000779 denotes conjugated
T10847 17880-17886 NCBITaxon:9986 denotes rabbit
T10848 17896-17901 NCBITaxon:10088 denotes mouse
T10849 17902-17917 GO:0019814 denotes immunoglobulins
T10850 17927-17931 CL_GO_EXT:cell denotes Cell
T10851 17932-17941 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes Signaling
T10852 17963-17971 GO:0042571 denotes antibody
T10853 17972-17979 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T10854 17995-18000 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes bound
T10855 18001-18009 GO:0042571 denotes antibody
T11434 18083-18087 PR_EXT:000006847 denotes ESG1
T11435 18098-18106 CL:0002322 denotes ES cells
T11436 18101-18106 CL_GO_EXT:cell denotes cells
T11437 18112-18123 UBERON:0000358 denotes blastocysts
T11438 18124-18128 PR_EXT:000006847 denotes Esg1
T11439 18128-18129 SO_EXT:normal_or_wild_type_or_present denotes +
T11440 18130-18131 SO_EXT:sequence_nullness_or_absence denotes -
T11441 18134-18138 PR_EXT:000006847 denotes ESG1
T11442 18138-18139 SO_EXT:sequence_nullness_or_absence denotes -
T11443 18140-18141 SO_EXT:sequence_nullness_or_absence denotes -
T11444 18142-18148 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T11445 18149-18155 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes female
T11446 18156-18160 NCBITaxon:10088 denotes mice
T11447 18180-18189 CHEBI:41774 denotes Tamoxifen
T11448 18202-18214 CHEBI:6716 denotes Depo-provera
T11449 18225-18236 UBERON_EXT:zone_of_skin_or_skin_of_body denotes cutaneously
T11450 18257-18266 GO:0007565 denotes pregnancy
T11451 18285-18292 UBERON:0000922 denotes embryos
T11452 18329-18335 UBERON:0000995 denotes uterus
T11453 18363-18368 CL_GO_EXT:cell denotes cells
T11454 18460-18466 NCBITaxon:27592 denotes Bovine
T11455 18467-18472 UBERON:0001977 denotes Serum
T11456 18505-18516 CHEBI_SO_EXT:amino_acid denotes Amino Acids
T11457 18536-18547 CHEBI_EXT:L_glutamine denotes L-glutamine
T11458 18538-18547 CHEBI_SO_EXT:glutamine denotes glutamine
T11459 18570-18580 CHEBI:17334 denotes Penicillin
T11460 18581-18593 CHEBI:17076 denotes Streptomysin
T11461 18620-18637 CHEBI:41218 denotes 2-mercaptoethanol
T11462 18755-18762 PR_EXT:000027795 denotes trypsin
T11463 18786-18790 CL_GO_EXT:cell denotes cell
T11464 18786-18795 UBERON_EXT:cell_clump_or_cluster_or_group_or_mass_or_population denotes cell mass
T11465 18907-18914 PR_EXT:000027795 denotes trypsin
T11466 18920-18925 CL_GO_EXT:cell denotes cells
T11467 18938-18948 GO_EXT:biological_movement_or_translocation_process denotes transfered
T11468 19013-19020 _FRAGMENT denotes primary
T11469 19024-19028 CL:0000001 denotes cell
T11470 19021-19028 CL:0002322 denotes ES cell
T11471 19024-19028 CL_GO_EXT:cell denotes cell
T11472 19118-19122 CL_GO_EXT:cell denotes cell
T11473 19118-19129 UBERON:0000119 denotes cell layers
T11474 19143-19148 CL_GO_EXT:cell denotes cells
T11475 19166-19180 GO_EXT:trypsinization denotes trypsinization
T11866 19223-19226 CHEBI_SO_EXT:RNA denotes RNA
T11867 19232-19241 SO_EXT:wild_type_entity_or_quality denotes wild-type
T11868 19242-19250 CL:0002322 denotes ES cells
T11869 19245-19250 CL_GO_EXT:cell denotes cells
T11870 19255-19259 PR_EXT:000006847 denotes ESG1
T11871 19259-19260 SO_EXT:sequence_nullness_or_absence denotes -
T11872 19261-19262 SO_EXT:sequence_nullness_or_absence denotes -
T11873 19263-19271 CL:0002322 denotes ES cells
T11874 19266-19271 CL_GO_EXT:cell denotes cells
T11875 19276-19283 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T11876 19289-19292 CHEBI:37987 denotes Cy3
T11877 19297-19300 CHEBI:37989 denotes Cy5
T11878 19333-19343 GO:0097617 denotes hybridized
T11879 19349-19354 NCBITaxon:10088 denotes Mouse
T11880 19503-19516 GO:0097617 denotes Hybridization
T11881 19551-19557 SO_EXT:sequence_cloned_entity denotes clones
T11882 19612-19620 OBI_SO_EXT:gene_or_genetic_investigation_or_genetic_process denotes Genetics
T12044 3250-3254 PR_EXT:000006847 denotes ESG1
T12045 3254-3265 SO_EXT:0000336 denotes pseudogenes
T12046 3298-3303 NCBITaxon:10088 denotes mouse
T12047 3304-3311 SO_EXT:0001026 denotes genomic
T12048 3323-3335 SO_EXT:sequence_substitution_entity_or_process denotes Substitution
T12049 3336-3345 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T12050 3376-3382 SO_EXT:0000188 denotes Intron
T12051 3388-3391 SO_EXT:0000730 denotes gap
T12052 3392-3401 SO_EXT:biological_sequence denotes sequences
T12053 3432-3443 GO_SO_EXT:chromosome denotes Chromosomal
T12054 3444-3457 GO_PATO_EXT:biological_localization_process_or_quality denotes localizations
T12250 4927-4930 SO_EXT:0000153 denotes BAC
T12251 4931-4937 SO_EXT:sequence_cloned_entity denotes clones
T12252 4953-4957 PR_EXT:000006847 denotes ESG1
T12253 4958-4962 SO_EXT:0000704 denotes gene
T12254 4969-4980 SO_EXT:sequence_duplication_entity_or_process denotes duplication
T12255 4981-4991 SO_EXT:0000336 denotes pseudogene
T12256 4993-4995 SO_EXT:0000336 denotes PS
T12257 5001-5013 GO_PATO_EXT:biological_localization_process_or_quality denotes Localization
T12258 5021-5025 SO_EXT:0000704 denotes gene
T12259 5030-5032 SO_EXT:0000336 denotes PS
T12260 5036-5046 GO_SO_EXT:chromosome denotes chromosome
T12261 5053-5061 SO_EXT:biological_sequence denotes Sequence
T12262 5080-5084 SO_EXT:0000704 denotes gene
T12263 5089-5091 SO_EXT:0000336 denotes PS
T12264 5116-5126 SO:0000857 denotes homologous
T12265 5138-5142 CHEBI_SO_EXT:base denotes base
T12266 5138-5148 SO_EXT:0000028 denotes base pairs
T12267 5149-5157 SO_EXT:sequence_upstreamness denotes upstream
T12268 5171-5175 SO_EXT:0000147 denotes exon
T12269 5183-5185 SO_EXT:0000028 denotes bp
T12270 5186-5196 SO_EXT:sequence_downstreamness denotes downstream
T12271 5210-5214 SO_EXT:0000147 denotes exon
T12272 5216-5228 SO_EXT:sequence_substitution_entity_or_process denotes Substitution
T12273 5229-5238 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T12274 5273-5282 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T12522 5327-5335 SO_EXT:0000167 denotes Promoter
T12523 5336-5344 SO_EXT:0000165 denotes enhancer
T12524 5361-5365 PR_EXT:000006847 denotes ESG1
T12525 5366-5370 SO_EXT:0000704 denotes gene
T12526 5375-5385 SO_EXT:0000336 denotes pseudogene
T12527 5387-5390 CHEBI_SO_EXT:DNA denotes DNA
T12528 5408-5410 SO_EXT:0000028 denotes bp
T12529 5429-5448 SO_EXT:0001416 denotes 5' flanking regions
T12530 5456-5460 SO_EXT:0000704 denotes gene
T12531 5465-5467 SO_EXT:0000336 denotes PS
T12532 5473-5484 GO_EXT:biological_movement_or_translocation_process denotes transferred
T12533 5490-5500 GO_EXT:0045289 denotes luciferase
T12534 5510-5518 SO_EXT:0000155 denotes plasmids
T12535 5547-5552 SO_EXT:0000704 denotes genes
T12536 5560-5574 GO_PATO_RO_EXT:developmental_differentiation_process_or_quality denotes differentiated
T12537 5575-5583 CL:0002322 denotes ES cells
T12538 5578-5583 CL_GO_EXT:cell denotes cells
T12539 5600-5613 CHEBI:26536 denotes retinoic acid
T12540 5622-5630 CL:0002322 denotes ES cells
T12541 5625-5630 CL_GO_EXT:cell denotes cells
T12542 5658-5663 CL_GO_EXT:cell denotes cells
T13029 7577-7582 NCBITaxon:10088 denotes mouse
T13030 7583-7587 PR_EXT:000006847 denotes ESG1
T13031 7588-7592 SO_EXT:0000704 denotes gene
T13032 7666-7683 SO_EXT:0000409 denotes Recognition sites
T13033 7769-7773 SO_EXT:0000704 denotes gene
T13034 7774-7782 SO_EXT:sequence_coding_function denotes encoding
T13035 7794-7799 CHEBI_EXT:27026 denotes toxin
T13036 7812-7820 SO_EXT:sequence_insertion_process denotes inserted
T13037 7842-7859 SO_EXT:0001644 denotes targeting vectors
T13038 7942-7955 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T13039 7957-7959 SO_EXT:wild_type_entity_or_quality denotes WT
T13040 7961-7970 SO_EXT:wild_type_entity_or_quality denotes wild-type
T13041 7971-7979 CL:0002322 denotes ES cells
T13042 7974-7979 CL_GO_EXT:cell denotes cells
T13043 7984-7989 GO_EXT:beta_geo denotes β-geo
T13044 7990-7991 SO_EXT:normal_or_wild_type_or_present denotes +
T13045 7992-7993 SO_EXT:sequence_nullness_or_absence denotes -
T13046 7994-8002 CL:0002322 denotes ES cells
T13047 7997-8002 CL_GO_EXT:cell denotes cells
T13048 8012-8013 SO_EXT:normal_or_wild_type_or_present denotes +
T13049 8014-8015 SO_EXT:sequence_nullness_or_absence denotes -
T13050 8016-8024 CL:0002322 denotes ES cells
T13051 8019-8024 CL_GO_EXT:cell denotes cells
T13052 8026-8027 SO_EXT:sequence_nullness_or_absence denotes -
T13053 8028-8029 SO_EXT:sequence_nullness_or_absence denotes -
T13054 8031-8035 PR_EXT:000006847 denotes ESG1
T13055 8036-8040 SO_EXT:sequence_nullness denotes null
T13056 8041-8049 CL:0002322 denotes ES cells
T13057 8044-8049 CL_GO_EXT:cell denotes cells
T13058 8068-8073 SO_EXT:sequence_cloned_entity denotes clone
T13059 8145-8154 SO_EXT:wild_type_entity_or_quality denotes wild-type
T13060 8155-8163 CL:0002322 denotes ES cells
T13061 8158-8163 CL_GO_EXT:cell denotes cells
T13062 8165-8167 SO_EXT:wild_type_entity_or_quality denotes WT
T13063 8170-8174 PR_EXT:000006847 denotes ESG1
T13064 8175-8179 SO_EXT:sequence_nullness denotes null
T13065 8180-8188 CL:0002322 denotes ES cells
T13066 8183-8188 CL_GO_EXT:cell denotes cells
T13067 8190-8191 SO_EXT:sequence_nullness_or_absence denotes -
T13068 8192-8193 SO_EXT:sequence_nullness_or_absence denotes -
T13069 8201-8207 SO_EXT:sequence_cloned_entity denotes clones
T13070 8226-8234 CL:0002322 denotes ES cells
T13071 8229-8234 CL_GO_EXT:cell denotes cells
T13072 8236-8237 SO_EXT:normal_or_wild_type_or_present denotes +
T13073 8238-8239 SO_EXT:sequence_nullness_or_absence denotes -
T13074 8343-8346 CHEBI_SO_EXT:RNA denotes RNA
T13075 8348-8364 CHEBI:4883 denotes ethidium bromide
T13076 8377-8386 GO:0005840 denotes ribosomal
T13077 8377-8390 CHEBI_SO_EXT:rRNA denotes ribosomal RNA
T13551 9480-9484 PR_EXT:000006847 denotes ESG1
T13552 9485-9489 SO_EXT:sequence_nullness denotes null
T13553 9490-9498 CL:0002322 denotes ES cells
T13554 9493-9498 CL_GO_EXT:cell denotes cells
T13555 9521-9525 PR_EXT:000006847 denotes ESG1
T13556 9526-9530 SO_EXT:sequence_nullness denotes null
T13557 9531-9538 CL:0002322 denotes ES cell
T13558 9534-9538 CL_GO_EXT:cell denotes cell
T13559 9548-9553 GO:0040007 denotes grown
T13560 9568-9573 CL_GO_EXT:cell denotes cells
T13561 9578-9584 GO_EXT:biological_growth_entity_or_process denotes Growth
T13562 9594-9603 SO_EXT:wild_type_entity_or_quality denotes wild-type
T13563 9605-9607 SO_EXT:wild_type_entity_or_quality denotes WT
T13564 9610-9614 PR_EXT:000006847 denotes ESG1
T13565 9615-9619 SO_EXT:sequence_nullness denotes null
T13566 9621-9622 SO_EXT:sequence_nullness_or_absence denotes -
T13567 9623-9624 SO_EXT:sequence_nullness_or_absence denotes -
T13568 9644-9645 SO_EXT:normal_or_wild_type_or_present denotes +
T13569 9646-9647 SO_EXT:sequence_nullness_or_absence denotes -
T13570 9649-9657 CL:0002322 denotes ES cells
T13571 9652-9657 CL_GO_EXT:cell denotes cells
T13572 9664-9669 SO_EXT:sequence_cloned_entity denotes clone
T13573 9679-9684 CL_GO_EXT:cell denotes cells
T13574 9721-9725 CL_GO_EXT:cell denotes Cell
T13575 9802-9803 SO_EXT:normal_or_wild_type_or_present denotes +
T13576 9804-9805 SO_EXT:sequence_nullness_or_absence denotes -
T13577 9810-9811 SO_EXT:sequence_nullness_or_absence denotes -
T13578 9812-9813 SO_EXT:sequence_nullness_or_absence denotes -
T13579 9814-9819 CL_GO_EXT:cell denotes cells
T13580 9887-9893 SO_EXT:sequence_cloned_entity denotes clones
T13581 9933-9937 PR_EXT:000006847 denotes ESG1
T13582 9938-9942 SO_EXT:sequence_nullness denotes null
T13583 9943-9951 CL:0002322 denotes ES cells
T13584 9946-9951 CL_GO_EXT:cell denotes cells
T13585 9953-9964 CHEBI:51686 denotes hematoxylin
T13586 9967-9972 CHEBI_EXT:eosin denotes eosin
T13928 10875-10879 SO_EXT:0000704 denotes Gene
T13929 10875-10890 GO:0010467 denotes Gene expression
T13930 10903-10907 PR_EXT:000006847 denotes ESG1
T13931 10908-10912 SO_EXT:sequence_nullness denotes null
T13932 10913-10921 CL:0002322 denotes ES cells
T13933 10916-10921 CL_GO_EXT:cell denotes cells
T13934 10926-10929 CHEBI_SO_EXT:DNA denotes DNA
T13935 10957-10960 CHEBI_SO_EXT:RNA denotes RNA
T13936 10966-10975 SO_EXT:wild_type_entity_or_quality denotes wild-type
T13937 10976-10984 CL:0002322 denotes ES cells
T13938 10979-10984 CL_GO_EXT:cell denotes cells
T13939 10989-10993 PR_EXT:000006847 denotes ESG1
T13940 10994-10998 SO_EXT:sequence_nullness denotes null
T13941 10999-11007 CL:0002322 denotes ES cells
T13942 11002-11007 CL_GO_EXT:cell denotes cells
T13943 11013-11020 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T13944 11026-11029 CHEBI:37987 denotes Cy3
T13945 11034-11037 CHEBI:37989 denotes Cy5
T13946 11066-11076 GO:0097617 denotes hybridized
T13947 11088-11093 NCBITaxon:10088 denotes Mouse
T13948 11153-11159 SO_EXT:sequence_cloned_entity denotes clones
T13949 11197-11201 CL_GO_EXT:cell denotes Cell
T13950 11215-11219 PR_EXT:000006847 denotes ESG1
T13951 11219-11220 SO_EXT:normal_or_wild_type_or_present denotes +
T13952 11221-11222 SO_EXT:sequence_nullness_or_absence denotes -
T13953 11227-11231 PR_EXT:000006847 denotes ESG1
T13954 11231-11232 SO_EXT:sequence_nullness_or_absence denotes -
T13955 11233-11234 SO_EXT:sequence_nullness_or_absence denotes -
T13956 11235-11240 CL_GO_EXT:cell denotes cells
T13957 11259-11269 GO:0010467 denotes expression
T13958 11273-11277 PR_EXT:000006847 denotes ESG1
T13959 11279-11283 PR_EXT:000013044 denotes Oct3
T13960 11290-11294 PR_EXT:000005257 denotes CDK4
T3969 4906-4910 PR_EXT:000006847 denotes ESG1
T3970 4911-4915 SO_EXT:0000704 denotes gene
T3971 5795-5798 SO_EXT:0000153 denotes BAC
T3992 6211-6221 SO_EXT:0000028 denotes base pairs
T3993 6229-6248 SO_EXT:0001417 denotes 3' flanking regions
T3882 2445-2449 SO_EXT:0000704 denotes gene
T3883 2454-2464 SO_EXT:0000336 denotes psedogenes
T3884 2482-2493 GO_SO_EXT:chromosome denotes chromosomal
T3885 2494-2507 GO_PATO_EXT:biological_localization_process_or_quality denotes localizations
T3886 2515-2520 NCBITaxon:10088 denotes mouse
T3887 2521-2525 PR_EXT:000006847 denotes ESG1
T3888 2526-2530 SO_EXT:0000704 denotes gene
T3889 2535-2546 SO_EXT:0000336 denotes pseudogenes
T3890 2585-2590 NCBITaxon:10088 denotes mouse
T3891 2591-2598 SO_EXT:0001026 denotes genomic
T3892 2617-2621 PR_EXT:000006847 denotes ESG1
T3893 2622-2626 SO_EXT:cDNA denotes cDNA
T3955 4555-4576 SO_EXT:0001421 denotes exon-intron junctions
T3956 4587-4605 SO_EXT:0001416 denotes 5' flanking region
T3957 4614-4617 CHEBI_SO_EXT:DNA denotes DNA
T3958 4644-4652 SO_EXT:0000167 denotes promoter
T3959 4653-4661 SO_EXT:0000165 denotes enhancer
T3960 4674-4684 GO_EXT:0045289 denotes luciferase
T3961 4706-4720 GO_PATO_RO_EXT:developmental_differentiation_process_or_quality denotes differentiated
T3962 4721-4729 CL:0002322 denotes ES cells
T3963 4724-4729 CL_GO_EXT:cell denotes cells
T3964 4742-4755 CL:0002371 denotes somatic cells
T3965 4750-4755 CL_GO_EXT:cell denotes cells
T3966 4833-4848 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T3967 4852-4865 CHEBI:26536 denotes retinoic acid
T3968 4890-4898 SO_EXT:biological_sequence denotes sequence
T3972 5799-5805 SO_EXT:sequence_cloned_entity denotes clones
T3973 5824-5828 PR_EXT:000006847 denotes ESG1
T3974 5834-5842 SO_EXT:biological_sequence denotes sequence
T3975 5864-5873 SO_EXT:biological_sequence denotes sequences
T3976 5894-5896 SO_EXT:0000028 denotes bp
T3977 5897-5916 SO_EXT:0000605 denotes intergenic sequence
T3978 5960-5964 PR_EXT:000006847 denotes ESG1
T3979 5970-5978 SO_EXT:biological_sequence denotes sequence
T3980 6022-6027 SO_EXT:0000147 denotes exons
T3981 6032-6039 SO_EXT:0000188 denotes introns
T3982 6047-6051 PR_EXT:000006847 denotes ESG1
T3983 6052-6056 SO_EXT:0000704 denotes gene
T3984 6063-6071 SO_EXT:biological_sequence denotes sequence
T3985 6101-6111 CHEBI_SO_EXT:nucleotide denotes nucleotide
T3986 6112-6125 SO_EXT:sequence_substitution_entity_or_process denotes substitutions
T3987 6140-6145 SO_EXT:0000147 denotes exons
T3988 6154-6164 CHEBI_SO_EXT:nucleotide denotes nucleotide
T3989 6165-6174 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T3990 6178-6182 SO_EXT:0000147 denotes exon
T3991 6211-6215 CHEBI_SO_EXT:base denotes base
T3994 6254-6263 SO_EXT:biological_conservation_process_or_quality denotes conserved
T3995 6276-6280 PR_EXT:000006847 denotes ESG1
T3996 6281-6285 SO_EXT:0000704 denotes gene
T3997 6294-6304 SO_EXT:0000336 denotes pseudogene
T3998 6316-6320 CHEBI_SO_EXT:base denotes base
T3999 6316-6326 SO_EXT:0000028 denotes base pairs
T4000 6334-6352 SO_EXT:0001416 denotes 5' flanking region
T4001 6374-6392 SO_EXT:0001416 denotes 5' flanking region
T4002 6398-6400 SO_EXT:0000028 denotes bp
T4003 6422-6430 SO_EXT:0000167 denotes promoter
T4004 6431-6439 SO_EXT:0000165 denotes enhancer
T4005 6452-6462 GO_EXT:0045289 denotes luciferase
T4006 6521-6529 SO_EXT:biological_sequence denotes sequence
T4007 6533-6544 GO_EXT:transcription denotes transcribed
T4008 6548-6558 GO:0006412 denotes translated
T4009 6577-6584 CHEBI_PR_EXT:protein denotes protein
T4010 6591-6599 SO_EXT:biological_sequence denotes sequence
T4011 6620-6631 SO_EXT:sequence_duplication_entity_or_process denotes duplication
T4012 6632-6642 SO_EXT:0000336 denotes pseudogene
T4013 6694-6705 SO_EXT:0000336 denotes pseudogenes
T4014 6719-6723 SO_EXT:0000147 denotes exon
T4015 6724-6730 SO_EXT:0000188 denotes intron
T4016 6751-6755 PR_EXT:000006847 denotes ESG1
T6263 8443-8451 CL:0002322 denotes ES cells
T6264 8446-8451 CL_GO_EXT:cell denotes cells
T6265 8471-8476 GO_EXT:beta_geo denotes β-geo
T6266 8477-8483 SO_EXT:0000440 denotes vector
T6267 8507-8515 CL:0002322 denotes ES cells
T6268 8510-8515 CL_GO_EXT:cell denotes cells
T6269 8524-8528 CHEBI:42768 denotes G418
T6270 8579-8583 PR_EXT:000006847 denotes ESG1
T6271 8584-8592 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T6272 8659-8663 PR_EXT:000006847 denotes ESG1
T6273 8673-8678 CL_GO_EXT:cell denotes cells
T6274 8698-8704 SO_EXT:sequence_cloned_entity denotes clones
T6275 8710-8715 GO_EXT:beta_geo denotes β-geo
T6276 8716-8722 SO_EXT:0000440 denotes vector
T6277 8727-8735 SO_EXT:sequence_substitution_process denotes replaced
T6278 8745-8751 SO_EXT:0000440 denotes vector
T6279 8767-8772 CL_GO_EXT:cell denotes cells
T6280 8816-8822 SO_EXT:sequence_cloned_entity denotes clones
T6281 8828-8833 GO_EXT:beta_geo denotes β-geo
T6282 8834-8840 SO_EXT:0000440 denotes vector
T6283 8881-8885 PR_EXT:000006847 denotes ESG1
T6284 8885-8886 SO_EXT:sequence_nullness_or_absence denotes -
T6285 8887-8888 SO_EXT:sequence_nullness_or_absence denotes -
T6286 8889-8897 CL:0002322 denotes ES cells
T6287 8892-8897 CL_GO_EXT:cell denotes cells
T6288 8945-8950 CL_GO_EXT:cell denotes cells
T6289 8956-8968 GO:0008283 denotes proliferated
T6290 9032-9041 SO_EXT:wild_type_entity_or_quality denotes wild-type
T6291 9043-9048 CL_GO_EXT:cell denotes cells
T6292 9062-9066 PR_EXT:000006847 denotes ESG1
T6293 9066-9067 SO_EXT:sequence_nullness_or_absence denotes -
T6294 9068-9069 SO_EXT:sequence_nullness_or_absence denotes -
T6295 9070-9075 CL_GO_EXT:cell denotes cells
T6296 9076-9090 GO_PATO_RO_EXT:developmental_differentiation_process_or_quality denotes differentiated
T6297 9121-9147 PR_EXT:000009799 denotes leukemia inhibitory factor
T6298 9163-9176 CHEBI:26536 denotes retinoic acid
T6299 9228-9234 UBERON:0000180 denotes flanks
T6300 9243-9247 NCBITaxon:10088 denotes mice
T6308 9987-9988 CL:0002322 denotes e
T6309 9987-9988 CL_GO_EXT:cell denotes e
T6310 9988-9989 CHEBI:51686 denotes x
T6311 10000-10004 SO_EXT:0000704 denotes gene
T6312 10000-10015 GO:0010467 denotes gene expression
T6313 10028-10032 PR_EXT:000006847 denotes ESG1
T6314 10032-10033 SO_EXT:sequence_nullness_or_absence denotes -
T6315 10034-10035 SO_EXT:sequence_nullness_or_absence denotes -
T6316 10036-10044 CL:0002322 denotes ES cells
T6317 10039-10044 CL_GO_EXT:cell denotes cells
T6318 10051-10066 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotide
T6319 10073-10076 CHEBI_SO_EXT:DNA denotes DNA
T6320 10110-10115 SO_EXT:0000704 denotes genes
T6321 10142-10150 CL:0002322 denotes ES cells
T6322 10145-10150 CL_GO_EXT:cell denotes cells
T6323 10152-10156 PR_EXT:000006847 denotes ESG1
T6324 10179-10183 SO_EXT:0000704 denotes gene
T6325 10218-10222 PR_EXT:000006847 denotes ESG1
T6326 10222-10223 SO_EXT:sequence_nullness_or_absence denotes -
T6374 11322-11323 NCBITaxon:10088 denotes e
T6375 11328-11332 PR_EXT:000006847 denotes ESG1
T6376 11332-11333 SO_EXT:sequence_nullness_or_absence denotes -
T6377 11342-11346 NCBITaxon:10088 denotes mice
T6378 11360-11365 GO_EXT:beta_geo denotes β-geo
T6379 11366-11370 PR_EXT:000006847 denotes ESG1
T6380 11370-11371 SO_EXT:normal_or_wild_type_or_present denotes +
T6381 11372-11373 SO_EXT:sequence_nullness_or_absence denotes -
T6382 11374-11381 CL:0002322 denotes ES cell
T6383 11377-11381 CL_GO_EXT:cell denotes cell
T6384 11382-11388 SO_EXT:sequence_cloned_entity denotes clones
T6385 11398-11409 UBERON:0000358 denotes blastocysts
T6386 11420-11424 NCBITaxon:10088 denotes mice
T6387 11471-11477 SO_EXT:sequence_cloned_entity denotes clones
T6388 11491-11495 PR_EXT:000006847 denotes ESG1
T6389 11495-11496 SO_EXT:sequence_nullness_or_absence denotes -
T6390 11497-11498 SO_EXT:sequence_nullness_or_absence denotes -
T6391 11499-11503 NCBITaxon:10088 denotes mice
T6392 11532-11541 SO_EXT:wild_type_entity_or_quality denotes wild-type
T6393 11546-11550 PR_EXT:000006847 denotes ESG1
T6394 11550-11551 SO_EXT:normal_or_wild_type_or_present denotes +
T6395 11552-11553 SO_EXT:sequence_nullness_or_absence denotes -
T6396 11562-11566 PR_EXT:000006847 denotes ESG1
T6397 11566-11567 SO_EXT:sequence_nullness_or_absence denotes -
T6398 11568-11569 SO_EXT:sequence_nullness_or_absence denotes -
T6399 11592-11596 PR_EXT:000006847 denotes ESG1
T6400 11596-11597 SO_EXT:normal_or_wild_type_or_present denotes +
T6402 11600-11604 NCBITaxon:10088 denotes mice
R319 T2087 T2086 _lexicallyChainedTo cells,Embryonic stem
R320 T2087 T2088 _lexicallyChainedTo cells,ES
R321 T2110 T2109 _lexicallyChainedTo cells,proliferation of
R322 T2139 T2138 _lexicallyChainedTo cells,embryonic
R323 T2153 T2152 _lexicallyChainedTo cells,embryonic
R324 T2157 T2156 _lexicallyChainedTo stem cells,multipotent
R1328 T6245 T6244 _lexicallyChainedTo genes,fusion of
R2890 T10381 T10380 _lexicallyChainedTo antibodies,Generation of
R3167 T11469 T11468 _lexicallyChainedTo cell,primary

craft-sa-dev

Id Subject Object Predicate Lexical cue
T2208 11-20 JJ denotes Embryonic
T2209 21-25 NN denotes stem
T2210 31-36 NNS denotes cells
T2211 26-27 -LRB- denotes (
T2212 27-29 NN denotes ES
T2213 29-30 -RRB- denotes )
T2214 48-55 VBN denotes derived
T2215 37-41 VBD denotes were
T2216 42-47 RB denotes first
T2217 56-60 IN denotes from
T2218 61-64 DT denotes the
T2219 65-76 NNS denotes blastocysts
T2220 77-79 IN denotes of
T2221 80-84 NNS denotes mice
T2222 85-87 IN denotes in
T2223 88-92 CD denotes 1981
T2224 93-94 -LRB- denotes [
T2225 96-97 CD denotes 2
T2226 94-95 CD denotes 1
T2227 95-96 , denotes ,
T2228 97-98 -RRB- denotes ]
T2229 99-102 CC denotes and
T2230 103-109 NNS denotes humans
T2231 110-112 IN denotes in
T2232 113-117 CD denotes 1998
T2233 118-119 -LRB- denotes [
T2234 119-120 CD denotes 3
T2235 120-121 -RRB- denotes ]
T2236 121-122 . denotes .
T2237 122-292 sentence denotes ES cells have two important properties: theability to maintain pluripotency, which is the ability to differentiate into a wide variety of cells, and rapid proliferation.
T2238 123-125 NN denotes ES
T2239 126-131 NNS denotes cells
T2240 132-136 VBP denotes have
T2241 137-140 CD denotes two
T2242 151-161 NNS denotes properties
T2243 141-150 JJ denotes important
T2244 161-163 : denotes :
T2245 163-173 NN denotes theability
T2246 174-176 TO denotes to
T2247 177-185 VB denotes maintain
T2248 186-198 NN denotes pluripotency
T2249 198-200 , denotes ,
T2250 200-205 WDT denotes which
T2251 206-208 VBZ denotes is
T2252 209-212 DT denotes the
T2253 213-220 NN denotes ability
T2254 221-223 TO denotes to
T2255 224-237 VB denotes differentiate
T2256 238-242 IN denotes into
T2257 243-244 DT denotes a
T2258 250-257 NN denotes variety
T2259 245-249 JJ denotes wide
T2260 258-260 IN denotes of
T2261 261-266 NNS denotes cells
T2262 266-268 , denotes ,
T2263 268-271 CC denotes and
T2264 272-277 JJ denotes rapid
T2265 278-291 NN denotes proliferation
T2266 291-292 . denotes .
T2267 292-387 sentence denotes These characteristics make mouse ES cells an essential component of gene targeting technology.
T2268 293-298 DT denotes These
T2269 299-314 NNS denotes characteristics
T2270 315-319 VBP denotes make
T2271 320-325 NN denotes mouse
T2272 329-334 NNS denotes cells
T2273 326-328 NN denotes ES
T2274 348-357 NN denotes component
T2275 335-337 DT denotes an
T2276 338-347 JJ denotes essential
T2277 358-360 IN denotes of
T2278 361-365 NN denotes gene
T2279 366-375 NN denotes targeting
T2280 376-386 NN denotes technology
T2281 386-387 . denotes .
T2282 387-560 sentence denotes These qualitiesalso make human ES cells attractive sources for cell transplantation therapy to treat various diseases, including spinal cord injuries and juvenile diabetes.
T2283 388-393 DT denotes These
T2284 394-403 NNS denotes qualities
T2285 408-412 VBP denotes make
T2286 403-407 RB denotes also
T2287 413-418 JJ denotes human
T2288 422-427 NNS denotes cells
T2289 419-421 NN denotes ES
T2290 439-446 NNS denotes sources
T2291 428-438 JJ denotes attractive
T2292 447-450 IN denotes for
T2293 451-455 NN denotes cell
T2294 456-471 NN denotes transplantation
T2295 472-479 NN denotes therapy
T2296 480-482 TO denotes to
T2297 483-488 VB denotes treat
T2298 489-496 JJ denotes various
T2299 497-505 NNS denotes diseases
T2300 505-507 , denotes ,
T2301 507-516 VBG denotes including
T2302 517-523 JJ denotes spinal
T2303 524-528 NN denotes cord
T2304 529-537 NNS denotes injuries
T2305 538-541 CC denotes and
T2306 542-550 JJ denotes juvenile
T2307 551-559 NN denotes diabetes
T2308 559-560 . denotes .
T2309 560-718 sentence denotes The molecular mechanisms underlying the pluripotency and rapid proliferation of ES cells are currently a major focus of the field of stem cell biology [4-6].
T2310 561-564 DT denotes The
T2311 575-585 NNS denotes mechanisms
T2312 565-574 JJ denotes molecular
T2313 650-653 VBP denotes are
T2314 586-596 VBG denotes underlying
T2315 597-600 DT denotes the
T2316 601-613 NN denotes pluripotency
T2317 614-617 CC denotes and
T2318 618-623 JJ denotes rapid
T2319 624-637 NN denotes proliferation
T2320 638-640 IN denotes of
T2321 641-643 NN denotes ES
T2322 644-649 NNS denotes cells
T2323 654-663 RB denotes currently
T2324 664-665 DT denotes a
T2325 672-677 NN denotes focus
T2326 666-671 JJ denotes major
T2327 678-680 IN denotes of
T2328 681-684 DT denotes the
T2329 685-690 NN denotes field
T2330 691-693 IN denotes of
T2331 694-698 NN denotes stem
T2332 699-703 NN denotes cell
T2333 704-711 NN denotes biology
T2334 712-713 -LRB- denotes [
T2335 713-714 CD denotes 4
T2336 714-715 SYM denotes -
T2337 715-716 CD denotes 6
T2338 716-717 -RRB- denotes ]
T2339 717-718 . denotes .
T2340 718-908 sentence denotes To identify molecules essential in ES cells for these properties, several groups have utilized transcriptome analyses to identify genes specifically expressed in ES cells and early embryos.
T2341 719-721 TO denotes To
T2342 722-730 VB denotes identify
T2343 805-813 VBN denotes utilized
T2344 731-740 NNS denotes molecules
T2345 741-750 JJ denotes essential
T2346 751-753 IN denotes in
T2347 754-756 NN denotes ES
T2348 757-762 NNS denotes cells
T2349 763-766 IN denotes for
T2350 767-772 DT denotes these
T2351 773-783 NNS denotes properties
T2352 783-785 , denotes ,
T2353 785-792 JJ denotes several
T2354 793-799 NNS denotes groups
T2355 800-804 VBP denotes have
T2356 814-827 NN denotes transcriptome
T2357 828-836 NNS denotes analyses
T2358 837-839 TO denotes to
T2359 840-848 VB denotes identify
T2360 849-854 NNS denotes genes
T2361 855-867 RB denotes specifically
T2362 868-877 VBN denotes expressed
T2363 878-880 IN denotes in
T2364 881-883 NN denotes ES
T2365 884-889 NNS denotes cells
T2366 890-893 CC denotes and
T2367 894-899 JJ denotes early
T2368 900-907 NNS denotes embryos
T2369 907-908 . denotes .
T2370 908-1101 sentence denotes These analyses, including DNA microarray analyses [7] and expressed sequence tag analyses [8-12], identified several common transcripts, including ESG1 that was also designated dppa5 or ECAT2.
T2371 909-914 DT denotes These
T2372 915-923 NNS denotes analyses
T2373 1007-1017 VBD denotes identified
T2374 923-925 , denotes ,
T2375 925-934 VBG denotes including
T2376 935-938 NN denotes DNA
T2377 950-958 NNS denotes analyses
T2378 939-949 NN denotes microarray
T2379 959-960 -LRB- denotes [
T2380 960-961 CD denotes 7
T2381 961-962 -RRB- denotes ]
T2382 963-966 CC denotes and
T2383 967-976 VBN denotes expressed
T2384 977-985 NN denotes sequence
T2385 990-998 NNS denotes analyses
T2386 986-989 NN denotes tag
T2387 999-1000 -LRB- denotes [
T2388 1000-1001 CD denotes 8
T2389 1001-1002 SYM denotes -
T2390 1002-1004 CD denotes 12
T2391 1004-1005 -RRB- denotes ]
T2392 1005-1007 , denotes ,
T2393 1018-1025 JJ denotes several
T2394 1033-1044 NNS denotes transcripts
T2395 1026-1032 JJ denotes common
T2396 1044-1046 , denotes ,
T2397 1046-1055 VBG denotes including
T2398 1056-1060 NN denotes ESG1
T2399 1061-1065 WDT denotes that
T2400 1075-1085 VBN denotes designated
T2401 1066-1069 VBD denotes was
T2402 1070-1074 RB denotes also
T2403 1086-1091 NN denotes dppa5
T2404 1092-1094 CC denotes or
T2405 1095-1100 NN denotes ECAT2
T2406 1100-1101 . denotes .
T2407 1101-1229 sentence denotes ESG1 was originally identified as a transcript Ph34 that was down-regulated by retinoic acid in embryonic carcinoma cells [13].
T2408 1102-1106 NN denotes ESG1
T2409 1122-1132 VBN denotes identified
T2410 1107-1110 VBD denotes was
T2411 1111-1121 RB denotes originally
T2412 1133-1135 IN denotes as
T2413 1136-1137 DT denotes a
T2414 1149-1153 NN denotes Ph34
T2415 1138-1148 NN denotes transcript
T2416 1154-1158 WDT denotes that
T2417 1168-1177 VBN denotes regulated
T2418 1159-1162 VBD denotes was
T2419 1163-1167 RB denotes down
T2420 1167-1168 HYPH denotes -
T2421 1178-1180 IN denotes by
T2422 1181-1189 JJ denotes retinoic
T2423 1190-1194 NN denotes acid
T2424 1195-1197 IN denotes in
T2425 1198-1207 JJ denotes embryonic
T2426 1208-1217 NN denotes carcinoma
T2427 1218-1223 NNS denotes cells
T2428 1224-1225 -LRB- denotes [
T2429 1225-1227 CD denotes 13
T2430 1227-1228 -RRB- denotes ]
T2431 1228-1229 . denotes .
T2432 1229-1316 sentence denotes The expression of this gene was confined in mice to early embryos and germ cells [14].
T2433 1230-1233 DT denotes The
T2434 1234-1244 NN denotes expression
T2435 1262-1270 VBN denotes confined
T2436 1245-1247 IN denotes of
T2437 1248-1252 DT denotes this
T2438 1253-1257 NN denotes gene
T2439 1258-1261 VBD denotes was
T2440 1271-1273 IN denotes in
T2441 1274-1278 NNS denotes mice
T2442 1279-1281 IN denotes to
T2443 1282-1287 JJ denotes early
T2444 1288-1295 NNS denotes embryos
T2445 1296-1299 CC denotes and
T2446 1300-1304 NN denotes germ
T2447 1305-1310 NNS denotes cells
T2448 1311-1312 -LRB- denotes [
T2449 1312-1314 CD denotes 14
T2450 1314-1315 -RRB- denotes ]
T2451 1315-1316 . denotes .
T2452 1316-1443 sentence denotes It is also expressed in pluripotent cells, including ES cells, embryonic germ cells, and multipotent germline stem cells [15].
T2453 1317-1319 PRP denotes It
T2454 1328-1337 VBN denotes expressed
T2455 1320-1322 VBZ denotes is
T2456 1323-1327 RB denotes also
T2457 1338-1340 IN denotes in
T2458 1341-1352 JJ denotes pluripotent
T2459 1353-1358 NNS denotes cells
T2460 1358-1360 , denotes ,
T2461 1360-1369 VBG denotes including
T2462 1370-1372 NN denotes ES
T2463 1373-1378 NNS denotes cells
T2464 1378-1380 , denotes ,
T2465 1380-1389 JJ denotes embryonic
T2466 1395-1400 NNS denotes cells
T2467 1390-1394 NN denotes germ
T2468 1400-1402 , denotes ,
T2469 1402-1405 CC denotes and
T2470 1406-1417 JJ denotes multipotent
T2471 1432-1437 NNS denotes cells
T2472 1418-1426 NN denotes germline
T2473 1427-1431 NN denotes stem
T2474 1438-1439 -LRB- denotes [
T2475 1439-1441 CD denotes 15
T2476 1441-1442 -RRB- denotes ]
T2477 1442-1443 . denotes .
T2478 1443-1560 sentence denotes ESG1 encodes a polypeptide of 118 amino acids that contains a single KH domain, which is an RNA-binding domain [16].
T2479 1444-1448 NN denotes ESG1
T2480 1449-1456 VBZ denotes encodes
T2481 1457-1458 DT denotes a
T2482 1459-1470 NN denotes polypeptide
T2483 1471-1473 IN denotes of
T2484 1474-1477 CD denotes 118
T2485 1484-1489 NNS denotes acids
T2486 1478-1483 NN denotes amino
T2487 1490-1494 WDT denotes that
T2488 1495-1503 VBZ denotes contains
T2489 1504-1505 DT denotes a
T2490 1516-1522 NN denotes domain
T2491 1506-1512 JJ denotes single
T2492 1513-1515 NN denotes KH
T2493 1522-1524 , denotes ,
T2494 1524-1529 WDT denotes which
T2495 1530-1532 VBZ denotes is
T2496 1533-1535 DT denotes an
T2497 1548-1554 NN denotes domain
T2498 1536-1539 NN denotes RNA
T2499 1540-1547 VBG denotes binding
T2500 1539-1540 HYPH denotes -
T2501 1555-1556 -LRB- denotes [
T2502 1556-1558 CD denotes 16
T2503 1558-1559 -RRB- denotes ]
T2504 1559-1560 . denotes .
T2505 1560-1677 sentence denotes It remains unclear, however, if ESG1 functions as an RNA-binding protein or the roles it plays in ES cells and mice.
T2506 1561-1563 PRP denotes It
T2507 1564-1571 VBZ denotes remains
T2508 1572-1579 JJ denotes unclear
T2509 1579-1581 , denotes ,
T2510 1581-1588 RB denotes however
T2511 1588-1590 , denotes ,
T2512 1590-1592 IN denotes if
T2513 1598-1607 VBZ denotes functions
T2514 1593-1597 NN denotes ESG1
T2515 1608-1610 IN denotes as
T2516 1611-1613 DT denotes an
T2517 1626-1633 NN denotes protein
T2518 1614-1617 NN denotes RNA
T2519 1618-1625 VBG denotes binding
T2520 1617-1618 HYPH denotes -
T2521 1634-1636 CC denotes or
T2522 1637-1640 DT denotes the
T2523 1641-1646 NNS denotes roles
T2524 1647-1649 PRP denotes it
T2525 1650-1655 VBZ denotes plays
T2526 1656-1658 IN denotes in
T2527 1659-1661 NN denotes ES
T2528 1662-1667 NNS denotes cells
T2529 1668-1671 CC denotes and
T2530 1672-1676 NNS denotes mice
T2531 1676-1677 . denotes .
T2532 1677-1800 sentence denotes Previous genomic library screening by identified genomic clones containing the mouse ESG1 gene and seven pseudogenes [17].
T2533 1678-1686 JJ denotes Previous
T2534 1703-1712 NN denotes screening
T2535 1687-1694 JJ denotes genomic
T2536 1695-1702 NN denotes library
T2537 1713-1715 IN denotes by
T2538 1716-1726 VBN denotes identified
T2539 1735-1741 NNS denotes clones
T2540 1727-1734 JJ denotes genomic
T2541 1742-1752 VBG denotes containing
T2542 1753-1756 DT denotes the
T2543 1768-1772 NN denotes gene
T2544 1757-1762 NN denotes mouse
T2545 1763-1767 NN denotes ESG1
T2546 1773-1776 CC denotes and
T2547 1777-1782 CD denotes seven
T2548 1783-1794 NNS denotes pseudogenes
T2549 1795-1796 -LRB- denotes [
T2550 1796-1798 CD denotes 17
T2551 1798-1799 -RRB- denotes ]
T2552 1799-1800 . denotes .
T2553 1800-1932 sentence denotes Two of these pseudogenes exhibit a similar exon-intron structure as the ESG1 gene, indicating their generation by gene duplication.
T2554 1801-1804 CD denotes Two
T2555 1826-1833 VBP denotes exhibit
T2556 1805-1807 IN denotes of
T2557 1808-1813 DT denotes these
T2558 1814-1825 NNS denotes pseudogenes
T2559 1834-1835 DT denotes a
T2560 1856-1865 NN denotes structure
T2561 1836-1843 JJ denotes similar
T2562 1844-1848 NN denotes exon
T2563 1849-1855 NN denotes intron
T2564 1848-1849 HYPH denotes -
T2565 1866-1868 IN denotes as
T2566 1869-1872 DT denotes the
T2567 1878-1882 NN denotes gene
T2568 1873-1877 NN denotes ESG1
T2569 1882-1884 , denotes ,
T2570 1884-1894 VBG denotes indicating
T2571 1895-1900 PRP$ denotes their
T2572 1901-1911 NN denotes generation
T2573 1912-1914 IN denotes by
T2574 1915-1919 NN denotes gene
T2575 1920-1931 NN denotes duplication
T2576 1931-1932 . denotes .
T2577 1932-2072 sentence denotes The five remaining pseudogenes did not contain any introns, indicating that these were generated by retrotransposition of ESG1 transcripts.
T2578 1933-1936 DT denotes The
T2579 1952-1963 NNS denotes pseudogenes
T2580 1937-1941 CD denotes five
T2581 1942-1951 VBG denotes remaining
T2582 1972-1979 VB denotes contain
T2583 1964-1967 VBD denotes did
T2584 1968-1971 RB denotes not
T2585 1980-1983 DT denotes any
T2586 1984-1991 NNS denotes introns
T2587 1991-1993 , denotes ,
T2588 1993-2003 VBG denotes indicating
T2589 2004-2008 IN denotes that
T2590 2020-2029 VBN denotes generated
T2591 2009-2014 DT denotes these
T2592 2015-2019 VBD denotes were
T2593 2030-2032 IN denotes by
T2594 2033-2051 NN denotes retrotransposition
T2595 2052-2054 IN denotes of
T2596 2055-2059 NN denotes ESG1
T2597 2060-2071 NNS denotes transcripts
T2598 2071-2072 . denotes .
T2599 2072-2175 sentence denotes The chromosomal localizations of the mouse ESG1 gene and pseudogenes, however, have not been reported.
T2600 2073-2076 DT denotes The
T2601 2089-2102 NNS denotes localizations
T2602 2077-2088 JJ denotes chromosomal
T2603 2166-2174 VBN denotes reported
T2604 2103-2105 IN denotes of
T2605 2106-2109 DT denotes the
T2606 2121-2125 NN denotes gene
T2607 2110-2115 NN denotes mouse
T2608 2116-2120 NN denotes ESG1
T2609 2126-2129 CC denotes and
T2610 2130-2141 NNS denotes pseudogenes
T2611 2141-2143 , denotes ,
T2612 2143-2150 RB denotes however
T2613 2150-2152 , denotes ,
T2614 2152-2156 VBP denotes have
T2615 2157-2160 RB denotes not
T2616 2161-2165 VBN denotes been
T2617 2174-2175 . denotes .
T2618 2175-2283 sentence denotes In this study, we determined the structure of the mouse gene encoding this protein and related pseudogenes.
T2619 2176-2178 IN denotes In
T2620 2194-2204 VBD denotes determined
T2621 2179-2183 DT denotes this
T2622 2184-2189 NN denotes study
T2623 2189-2191 , denotes ,
T2624 2191-2193 PRP denotes we
T2625 2205-2208 DT denotes the
T2626 2209-2218 NN denotes structure
T2627 2219-2221 IN denotes of
T2628 2222-2225 DT denotes the
T2629 2232-2236 NN denotes gene
T2630 2226-2231 NN denotes mouse
T2631 2237-2245 VBG denotes encoding
T2632 2246-2250 DT denotes this
T2633 2251-2258 NN denotes protein
T2634 2259-2262 CC denotes and
T2635 2263-2270 JJ denotes related
T2636 2271-2282 NNS denotes pseudogenes
T2637 2282-2283 . denotes .
T2638 2283-2365 sentence denotes We also performed gene targeting to determine the physiological function of ESG1.
T2639 2284-2286 PRP denotes We
T2640 2292-2301 VBD denotes performed
T2641 2287-2291 RB denotes also
T2642 2302-2306 NN denotes gene
T2643 2307-2316 NN denotes targeting
T2644 2317-2319 TO denotes to
T2645 2320-2329 VB denotes determine
T2646 2330-2333 DT denotes the
T2647 2348-2356 NN denotes function
T2648 2334-2347 JJ denotes physiological
T2649 2357-2359 IN denotes of
T2650 2360-2364 NN denotes ESG1
T2651 2364-2365 . denotes .
T4020 2391-2402 JJ denotes Chromosomal
T4021 2403-2415 NN denotes localization
T4022 2416-2419 CC denotes and
T4023 2420-2430 NNS denotes structures
T4024 2431-2433 IN denotes of
T4025 2434-2439 NN denotes mouse
T4026 2445-2449 NN denotes gene
T4027 2440-2444 NN denotes ESG1
T4028 2450-2453 CC denotes and
T4029 2454-2464 NNS denotes psedogenes
T4030 2464-2647 sentence denotes To determine the chromosomal localizations of the mouse ESG1 gene and pseudogenes, we performed a Blast analysis of the mouse genomic database with the ESG1 cDNA sequence as a query.
T4031 2465-2467 TO denotes To
T4032 2468-2477 VB denotes determine
T4033 2551-2560 VBD denotes performed
T4034 2478-2481 DT denotes the
T4035 2494-2507 NNS denotes localizations
T4036 2482-2493 JJ denotes chromosomal
T4037 2508-2510 IN denotes of
T4038 2511-2514 DT denotes the
T4039 2526-2530 NN denotes gene
T4040 2515-2520 NN denotes mouse
T4041 2521-2525 NN denotes ESG1
T4042 2531-2534 CC denotes and
T4043 2535-2546 NNS denotes pseudogenes
T4044 2546-2548 , denotes ,
T4045 2548-2550 PRP denotes we
T4046 2561-2562 DT denotes a
T4047 2569-2577 NN denotes analysis
T4048 2563-2568 NNP denotes Blast
T4049 2578-2580 IN denotes of
T4050 2581-2584 DT denotes the
T4051 2599-2607 NN denotes database
T4052 2585-2590 NN denotes mouse
T4053 2591-2598 JJ denotes genomic
T4054 2608-2612 IN denotes with
T4055 2613-2616 DT denotes the
T4056 2627-2635 NN denotes sequence
T4057 2617-2621 NN denotes ESG1
T4058 2622-2626 NN denotes cDNA
T4059 2636-2638 IN denotes as
T4060 2639-2640 DT denotes a
T4061 2641-2646 NN denotes query
T4062 2646-2647 . denotes .
T4063 2647-2765 sentence denotes We identified several putative pseudogenes without introns on chromosomes 1, 5, 11, 12, 14, 16, 17, and X (Figure 1).
T4064 2648-2650 PRP denotes We
T4065 2651-2661 VBD denotes identified
T4066 2662-2669 JJ denotes several
T4067 2679-2690 NNS denotes pseudogenes
T4068 2670-2678 JJ denotes putative
T4069 2691-2698 IN denotes without
T4070 2699-2706 NNS denotes introns
T4071 2707-2709 IN denotes on
T4072 2710-2721 NNS denotes chromosomes
T4073 2752-2753 NN denotes X
T4074 2722-2723 CD denotes 1
T4075 2723-2725 , denotes ,
T4076 2725-2726 CD denotes 5
T4077 2726-2728 , denotes ,
T4078 2728-2730 CD denotes 11
T4079 2730-2732 , denotes ,
T4080 2732-2734 CD denotes 12
T4081 2734-2736 , denotes ,
T4082 2736-2738 CD denotes 14
T4083 2738-2740 , denotes ,
T4084 2740-2742 CD denotes 16
T4085 2742-2744 , denotes ,
T4086 2744-2746 CD denotes 17
T4087 2746-2748 , denotes ,
T4088 2748-2751 CC denotes and
T4089 2754-2755 -LRB- denotes (
T4090 2755-2761 NN denotes Figure
T4091 2762-2763 CD denotes 1
T4092 2763-2764 -RRB- denotes )
T4093 2764-2765 . denotes .
T4094 2765-2897 sentence denotes In addition, two intron-less pseudogenes were identified in DNA fragments for which the chromosomal localization remained unmapped.
T4122 2949-2951 IN denotes to
T4123 2952-2956 NN denotes ESG1
T4124 2957-2961 NN denotes cDNA
T4125 2961-2963 , denotes ,
T4126 2963-2967 PRP denotes they
T4127 2968-2973 MD denotes could
T4128 2974-2977 RB denotes not
T4129 2986-2996 JJ denotes functional
T4130 2997-3005 NN denotes proteins
T4131 3005-3007 , denotes ,
T4132 3007-3014 IN denotes because
T4133 3015-3017 IN denotes of
T4134 3018-3026 JJ denotes critical
T4135 3027-3036 NNS denotes mutations
T4136 3036-3037 . denotes .
T4137 3037-3145 sentence denotes This result suggests that there are a larger number of intron-less pseudogenes than previously anticipated.
T4138 3038-3042 DT denotes This
T4139 3043-3049 NN denotes result
T4140 3050-3058 VBZ denotes suggests
T4141 3059-3063 IN denotes that
T4142 3070-3073 VBP denotes are
T4143 3064-3069 EX denotes there
T4144 3074-3075 DT denotes a
T4145 3083-3089 NN denotes number
T4146 3076-3082 JJR denotes larger
T4147 3090-3092 IN denotes of
T4148 3093-3104 JJ denotes intron-less
T4149 3105-3116 NNS denotes pseudogenes
T4150 3117-3121 IN denotes than
T4151 3133-3144 VBN denotes anticipated
T4152 3122-3132 RB denotes previously
T4153 3144-3145 . denotes .
T4154 3145-3239 sentence denotes Existence of multiple retropseudogenes is a hallmark of pluripotent cell-specific genes [18].
T4155 3146-3155 NN denotes Existence
T4156 3185-3187 VBZ denotes is
T4157 3156-3158 IN denotes of
T4158 3159-3167 JJ denotes multiple
T4159 3168-3184 NNS denotes retropseudogenes
T4160 3188-3189 DT denotes a
T4161 3190-3198 NN denotes hallmark
T4162 3199-3201 IN denotes of
T4163 3202-3213 JJ denotes pluripotent
T4164 3228-3233 NNS denotes genes
T4165 3214-3218 NN denotes cell
T4166 3219-3227 JJ denotes specific
T4167 3218-3219 HYPH denotes -
T4168 3234-3235 -LRB- denotes [
T4169 3235-3237 CD denotes 18
T4170 3237-3238 -RRB- denotes ]
T4171 3238-3239 . denotes .
T4172 3239-3240 sentence denotes
T4187 3543-3547 NN denotes ESG1
T4188 3553-3557 WDT denotes that
T4189 3558-3566 VBD denotes included
T4190 3567-3570 CD denotes two
T4191 3580-3587 NNS denotes introns
T4192 3571-3579 JJ denotes putative
T4193 3587-3588 . denotes .
T4194 3588-3696 sentence denotes These putative first and second exons, however, contained (4) multiple mutations of the ESG1 cDNA sequence.
T4195 3589-3594 DT denotes These
T4196 3621-3626 NNS denotes exons
T4197 3595-3603 JJ denotes putative
T4198 3604-3609 JJ denotes first
T4199 3610-3613 CC denotes and
T4200 3614-3620 JJ denotes second
T4201 3637-3646 VBD denotes contained
T4202 3626-3628 , denotes ,
T4203 3628-3635 RB denotes however
T4204 3635-3637 , denotes ,
T4205 3647-3648 -LRB- denotes (
T4206 3648-3649 CD denotes 4
T4207 3649-3650 -RRB- denotes )
T4208 3651-3659 JJ denotes multiple
T4209 3660-3669 NNS denotes mutations
T4210 3670-3672 IN denotes of
T4211 3673-3676 DT denotes the
T4212 3687-3695 NN denotes sequence
T4213 3677-3681 NN denotes ESG1
T4214 3682-3686 NN denotes cDNA
T4215 3695-3696 . denotes .
T4216 3696-3780 sentence denotes The putative third exon was identical to that of the previously reported ESG1 gene.
T4217 3697-3700 DT denotes The
T4218 3716-3720 NN denotes exon
T4219 3701-3709 JJ denotes putative
T4220 3710-3715 JJ denotes third
T4221 3721-3724 VBD denotes was
T4222 3725-3734 JJ denotes identical
T4223 3735-3737 IN denotes to
T4224 3738-3742 DT denotes that
T4225 3743-3745 IN denotes of
T4226 3746-3749 DT denotes the
T4227 3775-3779 NN denotes gene
T4228 3750-3760 RB denotes previously
T4229 3761-3769 VBN denotes reported
T4230 3770-3774 NN denotes ESG1
T4231 3779-3780 . denotes .
T4232 3780-3910 sentence denotes Also on chromosome 9, we identified another DNA fragment that was similar, but not identical, to the third exon of the ESG1 gene.
T4233 3781-3785 RB denotes Also
T4234 3806-3816 VBD denotes identified
T4235 3786-3788 IN denotes on
T4236 3789-3799 NN denotes chromosome
T4237 3800-3801 CD denotes 9
T4238 3801-3803 , denotes ,
T4239 3803-3805 PRP denotes we
T4240 3817-3824 DT denotes another
T4241 3829-3837 NN denotes fragment
T4242 3825-3828 NN denotes DNA
T4243 3838-3842 WDT denotes that
T4244 3843-3846 VBD denotes was
T4245 3847-3854 JJ denotes similar
T4246 3854-3856 , denotes ,
T4247 3856-3859 CC denotes but
T4248 3860-3863 RB denotes not
T4249 3864-3873 JJ denotes identical
T4250 3873-3875 , denotes ,
T4251 3875-3877 IN denotes to
T4252 3878-3881 DT denotes the
T4253 3888-3892 NN denotes exon
T4254 3882-3887 JJ denotes third
T4255 3893-3895 IN denotes of
T4256 3896-3899 DT denotes the
T4257 3905-3909 NN denotes gene
T4258 3900-3904 NN denotes ESG1
T4259 3909-3910 . denotes .
T4260 3910-4015 sentence denotes These findings suggest that these ESG1-like sequences on chromosome 9 have not been correctly assembled.
T4261 3911-3916 DT denotes These
T4262 3917-3925 NNS denotes findings
T4263 3926-3933 VBP denotes suggest
T4264 3934-3938 IN denotes that
T4265 4005-4014 VBN denotes assembled
T4266 3939-3944 DT denotes these
T4267 3955-3964 NNS denotes sequences
T4268 3945-3949 NN denotes ESG1
T4269 3950-3954 JJ denotes like
T4270 3949-3950 HYPH denotes -
T4271 3965-3967 IN denotes on
T4272 3968-3978 NN denotes chromosome
T4273 3979-3980 CD denotes 9
T4274 3981-3985 VBP denotes have
T4275 3986-3989 RB denotes not
T4276 3990-3994 VBN denotes been
T4277 3995-4004 RB denotes correctly
T4278 4014-4015 . denotes .
T4279 4015-4214 sentence denotes To obtain DNA fragments containing the ESG1 gene, we screened the bacterial artificial chromosome (BAC) DNA pool by PCR using primer pairs that would only amplify the real gene, not the pseudogenes.
T4280 4016-4018 TO denotes To
T4281 4019-4025 VB denotes obtain
T4282 4069-4077 VBD denotes screened
T4283 4026-4029 NN denotes DNA
T4284 4030-4039 NNS denotes fragments
T4285 4040-4050 VBG denotes containing
T4286 4051-4054 DT denotes the
T4287 4060-4064 NN denotes gene
T4288 4055-4059 NN denotes ESG1
T4289 4064-4066 , denotes ,
T4290 4066-4068 PRP denotes we
T4291 4078-4081 DT denotes the
T4292 4124-4128 NN denotes pool
T4293 4082-4091 JJ denotes bacterial
T4294 4103-4113 NN denotes chromosome
T4295 4092-4102 JJ denotes artificial
T4296 4114-4115 -LRB- denotes (
T4297 4115-4118 NN denotes BAC
T4298 4118-4119 -RRB- denotes )
T4299 4120-4123 NN denotes DNA
T4300 4129-4131 IN denotes by
T4301 4132-4135 NN denotes PCR
T4302 4136-4141 VBG denotes using
T4303 4142-4148 NN denotes primer
T4304 4149-4154 NNS denotes pairs
T4305 4155-4159 WDT denotes that
T4306 4171-4178 VB denotes amplify
T4307 4160-4165 MD denotes would
T4308 4166-4170 RB denotes only
T4309 4179-4182 DT denotes the
T4310 4188-4192 NN denotes gene
T4311 4183-4187 JJ denotes real
T4312 4192-4194 , denotes ,
T4313 4194-4197 RB denotes not
T4314 4202-4213 NNS denotes pseudogenes
T4315 4198-4201 DT denotes the
T4316 4213-4214 . denotes .
T4317 4214-4271 sentence denotes We obtained two independent, but overlapping BAC clones.
T4318 4215-4217 PRP denotes We
T4319 4218-4226 VBD denotes obtained
T4320 4227-4230 CD denotes two
T4321 4264-4270 NNS denotes clones
T4322 4231-4242 JJ denotes independent
T4323 4242-4244 , denotes ,
T4324 4244-4247 CC denotes but
T4325 4248-4259 VBG denotes overlapping
T4326 4260-4263 NN denotes BAC
T4327 4270-4271 . denotes .
T4328 4271-4463 sentence denotes Southern blot analyses and sequencing demonstrated that these clones contained a sequence exhibiting complete identity with ESG1 cDNA that was interrupted by two putative introns (Figure 2A).
T4329 4272-4280 NNP denotes Southern
T4330 4281-4285 NN denotes blot
T4331 4310-4322 VBD denotes demonstrated
T4332 4286-4294 NNS denotes analyses
T4333 4295-4298 CC denotes and
T4334 4299-4309 NN denotes sequencing
T4335 4323-4327 IN denotes that
T4336 4341-4350 VBD denotes contained
T4337 4328-4333 DT denotes these
T4338 4334-4340 NNS denotes clones
T4339 4351-4352 DT denotes a
T4340 4353-4361 NN denotes sequence
T4341 4362-4372 VBG denotes exhibiting
T4342 4373-4381 JJ denotes complete
T4343 4382-4390 NN denotes identity
T4344 4391-4395 IN denotes with
T4345 4396-4400 NN denotes ESG1
T4346 4401-4405 NN denotes cDNA
T4347 4406-4410 WDT denotes that
T4348 4415-4426 VBN denotes interrupted
T4349 4411-4414 VBD denotes was
T4350 4427-4429 IN denotes by
T4351 4430-4433 CD denotes two
T4352 4443-4450 NNS denotes introns
T4353 4434-4442 JJ denotes putative
T4354 4451-4452 -LRB- denotes (
T4355 4459-4461 NN denotes 2A
T4356 4452-4458 NN denotes Figure
T4357 4461-4462 -RRB- denotes )
T4358 4462-4463 . denotes .
T4359 4463-4582 sentence denotes The two intron sequences begin with GT and terminate with AG, fulfilling the GT-AG rule of exon-intron junctions [19].
T4360 4464-4467 DT denotes The
T4361 4479-4488 NNS denotes sequences
T4362 4468-4471 CD denotes two
T4363 4472-4478 NN denotes intron
T4364 4489-4494 VBP denotes begin
T4365 4495-4499 IN denotes with
T4366 4500-4502 NN denotes GT
T4367 4503-4506 CC denotes and
T4368 4507-4516 VB denotes terminate
T4369 4517-4521 IN denotes with
T4395 4609-4613 DT denotes this
T4396 4618-4626 NN denotes fragment
T4397 4614-4617 NN denotes DNA
T4398 4637-4643 JJ denotes strong
T4399 4662-4670 NN denotes activity
T4400 4644-4652 NN denotes promoter
T4401 4653-4661 NN denotes enhancer
T4402 4652-4653 HYPH denotes /
T4403 4671-4673 IN denotes by
T4404 4674-4684 NN denotes luciferase
T4405 4694-4700 NNS denotes assays
T4406 4685-4693 NN denotes reporter
T4407 4701-4703 IN denotes in
T4408 4704-4720 JJ denotes undifferentiated
T4409 4724-4729 NNS denotes cells
T4410 4721-4723 NN denotes ES
T4411 4729-4731 , denotes ,
T4412 4731-4734 CC denotes but
T4413 4735-4738 RB denotes not
T4414 4739-4741 IN denotes in
T4415 4742-4749 JJ denotes somatic
T4416 4750-4755 NNS denotes cells
T4417 4756-4757 -LRB- denotes (
T4418 4757-4763 NN denotes Figure
T4419 4764-4765 CD denotes 3
T4420 4765-4766 -RRB- denotes )
T4421 4766-4767 . denotes .
T4422 4767-4866 sentence denotes The same fragment showed much weaker activity after induction of differentiation by retinoic acid.
T4423 4768-4771 DT denotes The
T4424 4777-4785 NN denotes fragment
T4425 4772-4776 JJ denotes same
T4426 4786-4792 VBD denotes showed
T4427 4793-4797 RB denotes much
T4428 4798-4804 JJR denotes weaker
T4429 4805-4813 NN denotes activity
T4430 4814-4819 IN denotes after
T4431 4820-4829 NN denotes induction
T4432 4830-4832 IN denotes of
T4433 4833-4848 NN denotes differentiation
T4434 4849-4851 IN denotes by
T4435 4852-4860 JJ denotes retinoic
T4436 4861-4865 NN denotes acid
T4437 4865-4866 . denotes .
T4438 4866-4916 sentence denotes We concluded that this sequence is the ESG1 gene.
T4439 4867-4869 PRP denotes We
T4440 4870-4879 VBD denotes concluded
T4441 4880-4884 IN denotes that
T4442 4899-4901 VBZ denotes is
T4443 4885-4889 DT denotes this
T4444 4890-4898 NN denotes sequence
T4445 4902-4905 DT denotes the
T4446 4911-4915 NN denotes gene
T4447 4906-4910 NN denotes ESG1
T4448 4915-4916 . denotes .
T4487 5954-5955 . denotes .
T4488 5955-6057 sentence denotes The ESG1-like sequence exhibited greater than 95% identity to the exons and introns of the ESG1 gene.
T4489 5956-5959 DT denotes The
T4490 5970-5978 NN denotes sequence
T4491 5960-5964 NN denotes ESG1
T4492 5965-5969 JJ denotes like
T4493 5964-5965 HYPH denotes -
T4494 5979-5988 VBD denotes exhibited
T4495 5989-5996 JJR denotes greater
T4496 6002-6004 CD denotes 95
T4497 5997-6001 IN denotes than
T4498 6004-6005 NN denotes %
T4499 6006-6014 NN denotes identity
T4500 6015-6017 IN denotes to
T4501 6018-6021 DT denotes the
T4502 6022-6027 NNS denotes exons
T4503 6028-6031 CC denotes and
T4504 6032-6039 NNS denotes introns
T4505 6040-6042 IN denotes of
T4506 6043-6046 DT denotes the
T4507 6052-6056 NN denotes gene
T4508 6047-6051 NN denotes ESG1
T4509 6056-6057 . denotes .
T4510 6057-6197 sentence denotes This sequence, however, contained critical nucleotide substitutions in all of the exons and one nucleotide insertion in exon 2 (Figure 2B).
T4511 6058-6062 DT denotes This
T4512 6063-6071 NN denotes sequence
T4513 6082-6091 VBD denotes contained
T4514 6071-6073 , denotes ,
T4515 6073-6080 RB denotes however
T4516 6080-6082 , denotes ,
T4517 6092-6100 JJ denotes critical
T4518 6112-6125 NNS denotes substitutions
T4519 6101-6111 NN denotes nucleotide
T4520 6126-6128 IN denotes in
T4521 6129-6132 DT denotes all
T4522 6133-6135 IN denotes of
T4523 6136-6139 DT denotes the
T4524 6140-6145 NNS denotes exons
T4525 6146-6149 CC denotes and
T4526 6150-6153 CD denotes one
T4527 6165-6174 NN denotes insertion
T4528 6154-6164 NN denotes nucleotide
T4529 6175-6177 IN denotes in
T4530 6178-6182 NN denotes exon
T4531 6183-6184 CD denotes 2
T4532 6185-6186 -LRB- denotes (
T4533 6193-6195 NN denotes 2B
T4534 6186-6192 NN denotes Figure
T4535 6195-6196 -RRB- denotes )
T4536 6196-6197 . denotes .
T4537 6197-6368 sentence denotes Although 675 base pairs of the 3' flanking regions were conserved between the ESG1 gene and the pseudogene, only five base pairs of the 5' flanking region were identical.
T4538 6198-6206 IN denotes Although
T4539 6254-6263 VBN denotes conserved
T4540 6207-6210 CD denotes 675
T4541 6216-6221 NNS denotes pairs
T4542 6211-6215 NN denotes base
T4543 6222-6224 IN denotes of
T4544 6225-6228 DT denotes the
T4545 6241-6248 NNS denotes regions
T4546 6229-6230 CD denotes 3
T4547 6230-6231 SYM denotes '
T4548 6232-6240 NN denotes flanking
T4549 6249-6253 VBD denotes were
T4550 6353-6357 VBD denotes were
T4551 6264-6271 IN denotes between
T4552 6272-6275 DT denotes the
T4553 6281-6285 NN denotes gene
T4554 6276-6280 NN denotes ESG1
T4555 6286-6289 CC denotes and
T4556 6290-6293 DT denotes the
T4557 6294-6304 NN denotes pseudogene
T4558 6304-6306 , denotes ,
T4559 6306-6310 RB denotes only
T4560 6321-6326 NNS denotes pairs
T4561 6311-6315 CD denotes five
T4562 6316-6320 NN denotes base
T4563 6327-6329 IN denotes of
T4564 6330-6333 DT denotes the
T4565 6346-6352 NN denotes region
T4566 6334-6335 CD denotes 5
T4567 6335-6336 SYM denotes '
T4568 6337-6345 NN denotes flanking
T4569 6358-6367 JJ denotes identical
T4570 6367-6368 . denotes .
T4571 6368-6490 sentence denotes This 5' flanking region (~6 kbp) did not possess any promoter/enhancer activity in luciferase reporter assays (Figure 3).
T4572 6369-6373 DT denotes This
T4573 6386-6392 NN denotes region
T4574 6374-6375 CD denotes 5
T4575 6375-6376 SYM denotes '
T4576 6377-6385 NN denotes flanking
T4577 6410-6417 VB denotes possess
T4578 6393-6394 -LRB- denotes (
T4579 6397-6400 NN denotes kbp
T4580 6394-6395 SYM denotes ~
T4581 6395-6396 CD denotes 6
T4582 6400-6401 -RRB- denotes )
T4583 6402-6405 VBD denotes did
T4584 6406-6409 RB denotes not
T4585 6418-6421 DT denotes any
T4586 6440-6448 NN denotes activity
T4587 6422-6430 NN denotes promoter
T4588 6431-6439 NN denotes enhancer
T4589 6430-6431 HYPH denotes /
T4590 6449-6451 IN denotes in
T4591 6452-6462 NN denotes luciferase
T4592 6463-6471 NN denotes reporter
T4593 6472-6478 NNS denotes assays
T4594 6479-6480 -LRB- denotes (
T4595 6480-6486 NN denotes Figure
T4596 6487-6488 CD denotes 3
T4597 6488-6489 -RRB- denotes )
T4598 6489-6490 . denotes .
T4599 6490-6585 sentence denotes It is thus unlikely that this sequence is transcribed or translated into a functional protein.
T4600 6491-6493 PRP denotes It
T4601 6494-6496 VBZ denotes is
T4602 6497-6501 RB denotes thus
T4603 6502-6510 JJ denotes unlikely
T4604 6511-6515 IN denotes that
T4605 6533-6544 VBN denotes transcribed
T4606 6516-6520 DT denotes this
T4607 6521-6529 NN denotes sequence
T4608 6530-6532 VBZ denotes is
T4609 6545-6547 CC denotes or
T4610 6548-6558 VBN denotes translated
T4611 6559-6563 IN denotes into
T4612 6564-6565 DT denotes a
T4613 6577-6584 NN denotes protein
T4614 6566-6576 JJ denotes functional
T4615 6584-6585 . denotes .
T4616 6585-6643 sentence denotes This sequence likely represents a duplication pseudogene.
T4617 6586-6590 DT denotes This
T4618 6591-6599 NN denotes sequence
T4619 6607-6617 VBZ denotes represents
T4620 6600-6606 RB denotes likely
T4621 6618-6619 DT denotes a
T4622 6632-6642 NN denotes pseudogene
T4623 6620-6631 NN denotes duplication
T4624 6642-6643 . denotes .
T4625 6643-6766 sentence denotes Bierbaum previously reported the existence of two pseudogenes with similar exon-intron organization as the ESG1 gene [17].
T4626 6644-6652 NN denotes Bierbaum
T4627 6664-6672 VBD denotes reported
T4628 6653-6663 RB denotes previously
T4629 6673-6676 DT denotes the
T4630 6677-6686 NN denotes existence
T4631 6687-6689 IN denotes of
T4632 6690-6693 CD denotes two
T4633 6694-6705 NNS denotes pseudogenes
T4634 6706-6710 IN denotes with
T4635 6711-6718 JJ denotes similar
T4636 6731-6743 NN denotes organization
T4637 6719-6723 NN denotes exon
T4638 6724-6730 NN denotes intron
T4639 6723-6724 HYPH denotes -
T4640 6744-6746 IN denotes as
T4641 6747-6750 DT denotes the
T4642 6756-6760 NN denotes gene
T4643 6751-6755 NN denotes ESG1
T4644 6761-6762 -LRB- denotes [
T4645 6762-6764 CD denotes 17
T4646 6764-6765 -RRB- denotes ]
T4647 6765-6766 . denotes .
T4648 6766-6902 sentence denotes We could not determine which of these two pseudogenes corresponds to the one we identified or the location of the remaining pseudogene.
T4649 6767-6769 PRP denotes We
T4650 6780-6789 VB denotes determine
T4651 6770-6775 MD denotes could
T4652 6776-6779 RB denotes not
T4653 6790-6795 WDT denotes which
T4654 6821-6832 VBZ denotes corresponds
T4655 6796-6798 IN denotes of
T4656 6799-6804 DT denotes these
T4657 6809-6820 NNS denotes pseudogenes
T4658 6805-6808 CD denotes two
T4659 6833-6835 IN denotes to
T4660 6836-6839 DT denotes the
T4661 6840-6843 CD denotes one
T4662 6844-6846 PRP denotes we
T4663 6847-6857 VBD denotes identified
T4664 6858-6860 CC denotes or
T4665 6861-6864 DT denotes the
T4666 6865-6873 NN denotes location
T4667 6874-6876 IN denotes of
T4668 6877-6880 DT denotes the
T4669 6891-6901 NN denotes pseudogene
T4670 6881-6890 VBG denotes remaining
T4671 6901-6902 . denotes .
T6437 6904-6912 VBN denotes Targeted
T6438 6913-6923 NN denotes disruption
T6439 6924-6926 IN denotes of
T6440 6927-6930 DT denotes the
T6441 6942-6946 NN denotes gene
T6442 6931-6936 NN denotes mouse
T6443 6937-6941 NN denotes ESG1
T6444 6946-7044 sentence denotes To study the function of ESG1, we deleted the gene by homologous recombination in mouse ES cells.
T6445 6947-6949 TO denotes To
T6446 6950-6955 VB denotes study
T6447 6981-6988 VBD denotes deleted
T6448 6956-6959 DT denotes the
T6449 6960-6968 NN denotes function
T6450 6969-6971 IN denotes of
T6451 6972-6976 NN denotes ESG1
T6452 6976-6978 , denotes ,
T6453 6978-6980 PRP denotes we
T6454 6989-6992 DT denotes the
T6455 6993-6997 NN denotes gene
T6456 6998-7000 IN denotes by
T6457 7001-7011 JJ denotes homologous
T6458 7012-7025 NN denotes recombination
T6459 7026-7028 IN denotes in
T6460 7029-7034 NN denotes mouse
T6461 7038-7043 NNS denotes cells
T6462 7035-7037 NN denotes ES
T6463 7043-7044 . denotes .
T6464 7044-7283 sentence denotes We replaced the three exons with either a fusion of the neomycin-resistance and β-galactosidase genes (β-geo) or the hygromycin resistant gene (HygR) using two targeting vectors (Figure 4A) introduced into RF8 ES cells by electroporation.
T6465 7045-7047 PRP denotes We
T6466 7048-7056 VBD denotes replaced
T6467 7057-7060 DT denotes the
T6468 7067-7072 NNS denotes exons
T6469 7061-7066 CD denotes three
T6470 7073-7077 IN denotes with
T6471 7078-7084 CC denotes either
T6472 7087-7093 NN denotes fusion
T6473 7085-7086 DT denotes a
T6474 7094-7096 IN denotes of
T6475 7097-7100 DT denotes the
T6476 7141-7146 NNS denotes genes
T6477 7101-7109 NN denotes neomycin
T6478 7110-7120 NN denotes resistance
T6479 7109-7110 HYPH denotes -
T6480 7121-7124 CC denotes and
T6481 7125-7126 NN denotes β
T6482 7127-7140 NN denotes galactosidase
T6483 7126-7127 HYPH denotes -
T6484 7147-7148 -LRB- denotes (
T6485 7150-7153 NN denotes geo
T6486 7148-7149 NN denotes β
T6487 7149-7150 HYPH denotes -
T6488 7153-7154 -RRB- denotes )
T6489 7155-7157 CC denotes or
T6490 7158-7161 DT denotes the
T6491 7183-7187 NN denotes gene
T6517 7310-7316 NNS denotes clones
T6518 7302-7304 NN denotes ES
T6519 7305-7309 NN denotes cell
T6520 7317-7321 IN denotes with
T6521 7322-7329 JJ denotes correct
T6522 7341-7354 NN denotes recombination
T6523 7330-7340 JJ denotes homologous
T6524 7355-7357 IN denotes of
T6525 7358-7361 DT denotes the
T6526 7378-7384 NN denotes vector
T6527 7362-7363 NN denotes β
T6528 7364-7367 NN denotes geo
T6529 7363-7364 HYPH denotes -
T6530 7368-7377 NN denotes targeting
T6531 7384-7386 , denotes ,
T6532 7386-7391 WDT denotes which
T6533 7396-7405 VBN denotes confirmed
T6534 7392-7395 VBD denotes was
T6535 7406-7408 IN denotes by
T6536 7409-7417 NNP denotes Southern
T6537 7418-7422 NN denotes blot
T6538 7423-7431 NN denotes analysis
T6539 7432-7433 -LRB- denotes (
T6540 7440-7442 NN denotes 4B
T6541 7433-7439 NN denotes Figure
T6542 7442-7443 -RRB- denotes )
T6543 7443-7444 . denotes .
T6544 7444-7539 sentence denotes We obtained only one clone with correct homologous recombination of the HygR targeting vector.
T6545 7445-7447 PRP denotes We
T6546 7448-7456 VBD denotes obtained
T6547 7457-7461 RB denotes only
T6548 7466-7471 NN denotes clone
T6549 7462-7465 CD denotes one
T6550 7472-7476 IN denotes with
T6551 7477-7484 JJ denotes correct
T6552 7496-7509 NN denotes recombination
T6553 7485-7495 JJ denotes homologous
T6554 7510-7512 IN denotes of
T6555 7513-7516 DT denotes the
T6556 7532-7538 NN denotes vector
T6557 7517-7521 NN denotes HygR
T6558 7522-7531 NN denotes targeting
T6559 7538-7539 . denotes .
T6560 7539-7540 sentence denotes
T6561 8415-8419 sentence denotes To o
T6562 8415-8416 VBN denotes T
T6563 8416-8417 NN denotes o
T6564 8418-8419 IN denotes o
T6565 8419-8421 sentence denotes bt
T6566 8419-8420 IN denotes b
T6567 8420-8421 JJ denotes t
T6568 8421-8427 sentence denotes ain ho
T6569 8425-8427 VBN denotes ho
T6570 8421-8422 CC denotes a
T6611 8554-8556 , denotes ,
T6612 8556-8558 CD denotes 49
T6613 8564-8574 JJ denotes homozygous
T6614 8575-8578 IN denotes for
T6615 8579-8583 NN denotes ESG1
T6616 8584-8592 NN denotes deletion
T6617 8592-8593 . denotes .
T6618 8593-8691 sentence denotes Northern blot and western blot analyses confirmed the absence of ESG1 in these cells (Figure 4C).
T6619 8594-8602 NNP denotes Northern
T6620 8603-8607 NN denotes blot
T6621 8625-8633 NNS denotes analyses
T6622 8608-8611 CC denotes and
T6623 8612-8619 NNP denotes western
T6624 8620-8624 NN denotes blot
T6625 8634-8643 VBD denotes confirmed
T6626 8644-8647 DT denotes the
T6627 8648-8655 NN denotes absence
T6628 8656-8658 IN denotes of
T6629 8659-8663 NN denotes ESG1
T6630 8664-8666 IN denotes in
T6631 8667-8672 DT denotes these
T6632 8673-8678 NNS denotes cells
T6633 8679-8680 -LRB- denotes (
T6634 8687-8689 NN denotes 4C
T6635 8680-8686 NN denotes Figure
T6636 8689-8690 -RRB- denotes )
T6637 8690-8691 . denotes .
T6638 8691-8795 sentence denotes In 29 clones, the β-geo vector had replaced the HygR vector, such that the cells remained heterozygous.
T6639 8692-8694 IN denotes In
T6640 8727-8735 VBN denotes replaced
T6641 8695-8697 CD denotes 29
T6642 8698-8704 NNS denotes clones
T6643 8704-8706 , denotes ,
T6644 8706-8709 DT denotes the
T6645 8716-8722 NN denotes vector
T6646 8710-8711 NN denotes β
T6647 8712-8715 NN denotes geo
T6648 8711-8712 HYPH denotes -
T6649 8723-8726 VBD denotes had
T6650 8736-8739 DT denotes the
T6651 8745-8751 NN denotes vector
T6652 8740-8744 NN denotes HygR
T6653 8751-8753 , denotes ,
T6654 8753-8757 JJ denotes such
T6655 8773-8781 VBD denotes remained
T6656 8758-8762 IN denotes that
T6657 8763-8766 DT denotes the
T6658 8767-8772 NNS denotes cells
T6659 8782-8794 JJ denotes heterozygous
T6660 8794-8795 . denotes .
T6661 8795-8880 sentence denotes In the remaining 27 clones, the β-geo vector was integrated at non-homologous sites.
T6662 8796-8798 IN denotes In
T6663 8845-8855 VBN denotes integrated
T6664 8799-8802 DT denotes the
T6665 8816-8822 NNS denotes clones
T6666 8803-8812 VBG denotes remaining
T6667 8813-8815 CD denotes 27
T6668 8822-8824 , denotes ,
T6669 8824-8827 DT denotes the
T6670 8834-8840 NN denotes vector
T6671 8828-8829 NN denotes β
T6672 8830-8833 NN denotes geo
T6673 8829-8830 HYPH denotes -
T6674 8841-8844 VBD denotes was
T6675 8856-8858 IN denotes at
T6676 8859-8873 JJ denotes non-homologous
T6677 8874-8879 NNS denotes sites
T6678 8879-8880 . denotes .
T6679 8880-8938 sentence denotes ESG1-/- ES cells exhibited normal morphology (Figure 5A).
T6680 8881-8885 NN denotes ESG1
T6681 8892-8897 NNS denotes cells
T6682 8885-8886 SYM denotes -
T6683 8886-8887 HYPH denotes /
T6684 8887-8888 SYM denotes -
T6685 8889-8891 NN denotes ES
T6686 8898-8907 VBD denotes exhibited
T6687 8908-8914 JJ denotes normal
T6688 8915-8925 NN denotes morphology
T6689 8926-8927 -LRB- denotes (
T6690 8934-8936 NN denotes 5A
T6691 8927-8933 NN denotes Figure
T6692 8936-8937 -RRB- denotes )
T6693 8937-8938 . denotes .
T6694 8938-9061 sentence denotes These cells also proliferated at a speed comparable to that of the control (heterozygous and wild-type) cells (Figure 5B).
T6695 8939-8944 DT denotes These
T6696 8945-8950 NNS denotes cells
T6697 8956-8968 VBD denotes proliferated
T6698 8951-8955 RB denotes also
T6699 8969-8971 IN denotes at
T6700 8972-8973 DT denotes a
T6701 8974-8979 NN denotes speed
T6702 8980-8990 JJ denotes comparable
T6703 8991-8993 IN denotes to
T6704 8994-8998 DT denotes that
T6705 8999-9001 IN denotes of
T6706 9002-9005 DT denotes the
T6707 9043-9048 NNS denotes cells
T6708 9006-9013 NN denotes control
T6709 9014-9015 -LRB- denotes (
T6710 9015-9027 JJ denotes heterozygous
T6711 9028-9031 CC denotes and
T6712 9032-9036 JJ denotes wild
T6713 9037-9041 NN denotes type
T6714 9036-9037 HYPH denotes -
T6715 9041-9042 -RRB- denotes )
T6738 9149-9155 NN denotes Figure
T6739 9158-9159 -RRB- denotes )
T6740 9160-9162 CC denotes or
T6741 9163-9171 JJ denotes retinoic
T6742 9177-9186 NN denotes treatment
T6743 9172-9176 NN denotes acid
T6744 9187-9188 -LRB- denotes (
T6745 9192-9197 VBN denotes shown
T6746 9188-9191 RB denotes not
T6747 9197-9198 -RRB- denotes )
T6748 9198-9199 . denotes .
T6749 9199-9347 sentence denotes When transplanted into hind flanks of nude mice, these cells produced teratomas, tumors containing components of all three germ layers (Figure 5C).
T6750 9200-9204 WRB denotes When
T6751 9205-9217 VBN denotes transplanted
T6752 9261-9269 VBD denotes produced
T6753 9218-9222 IN denotes into
T6754 9223-9227 NN denotes hind
T6755 9228-9234 NNS denotes flanks
T6756 9235-9237 IN denotes of
T6757 9238-9242 JJ denotes nude
T6758 9243-9247 NNS denotes mice
T6759 9247-9249 , denotes ,
T6760 9249-9254 DT denotes these
T6761 9255-9260 NNS denotes cells
T6762 9270-9279 NNS denotes teratomas
T6763 9279-9281 , denotes ,
T6764 9281-9287 NNS denotes tumors
T6765 9288-9298 VBG denotes containing
T6766 9299-9309 NNS denotes components
T6767 9310-9312 IN denotes of
T6768 9313-9316 DT denotes all
T6769 9328-9334 NNS denotes layers
T6770 9317-9322 CD denotes three
T6771 9323-9327 NN denotes germ
T6772 9335-9336 -LRB- denotes (
T6773 9343-9345 NN denotes 5C
T6774 9336-9342 NN denotes Figure
T6775 9345-9346 -RRB- denotes )
T6776 9346-9347 . denotes .
T6777 9347-9457 sentence denotes These results indicate that ESG1 is dispensable for the self-renewal properties and pluripotency of ES cells.
T6778 9348-9353 DT denotes These
T6779 9354-9361 NNS denotes results
T6780 9362-9370 VBP denotes indicate
T6781 9371-9375 IN denotes that
T6782 9381-9383 VBZ denotes is
T6783 9376-9380 NN denotes ESG1
T6784 9384-9395 JJ denotes dispensable
T6785 9396-9399 IN denotes for
T6786 9400-9403 DT denotes the
T6787 9417-9427 NNS denotes properties
T6788 9404-9408 NN denotes self
T6789 9409-9416 NN denotes renewal
T6790 9408-9409 HYPH denotes -
T6791 9428-9431 CC denotes and
T6792 9432-9444 NN denotes pluripotency
T6793 9445-9447 IN denotes of
T6794 9448-9450 NN denotes ES
T6795 9451-9456 NNS denotes cells
T6796 9456-9457 . denotes .
T6797 9457-9458 sentence denotes
T6815 10033-10034 HYPH denotes /
T6816 10034-10035 SYM denotes -
T6817 10036-10038 NN denotes ES
T6818 10045-10050 VBG denotes using
T6819 10051-10066 NN denotes oligonucleotide
T6820 10067-10072 VBN denotes based
T6821 10066-10067 HYPH denotes -
T6822 10077-10088 NNS denotes microarrays
T6823 10073-10076 NN denotes DNA
T6824 10089-10101 VBG denotes representing
T6825 10102-10103 SYM denotes ~
T6826 10103-10109 CD denotes 20,000
T6827 10110-10115 NNS denotes genes
T6828 10115-10116 . denotes .
T6829 10116-10247 sentence denotes In comparison to control ES cells, ESG1 was identified as the gene reduced to the greatest extent in ESG1-/- ES cells (Figure 6A).
T6830 10117-10119 IN denotes In
T6831 10161-10171 VBN denotes identified
T6832 10120-10130 NN denotes comparison
T6833 10131-10133 IN denotes to
T6834 10134-10141 NN denotes control
T6835 10145-10150 NNS denotes cells
T6836 10142-10144 NN denotes ES
T6837 10150-10152 , denotes ,
T6838 10152-10156 NN denotes ESG1
T6839 10157-10160 VBD denotes was
T6840 10172-10174 IN denotes as
T6841 10175-10178 DT denotes the
T6842 10179-10183 NN denotes gene
T6843 10184-10191 VBN denotes reduced
T6844 10192-10194 IN denotes to
T6845 10195-10198 DT denotes the
T6846 10208-10214 NN denotes extent
T6847 10199-10207 JJS denotes greatest
T6848 10215-10217 IN denotes in
T6849 10218-10222 NN denotes ESG1
T6850 10229-10234 NNS denotes cells
T6851 10222-10223 SYM denotes -
T6852 10223-10224 HYPH denotes /
T6853 10224-10225 SYM denotes -
T6854 10226-10228 NN denotes ES
T6855 10235-10236 -LRB- denotes (
T6856 10243-10245 NN denotes 6A
T6857 10236-10242 NN denotes Figure
T6858 10245-10246 -RRB- denotes )
T6859 10246-10247 . denotes .
T6860 10247-10345 sentence denotes The expression of ES cell marker genes, such as Nanog and Oct3/4, was normal in ESG1-/- ES cells.
T6861 10248-10251 DT denotes The
T6862 10252-10262 NN denotes expression
T6863 10314-10317 VBD denotes was
T6864 10263-10265 IN denotes of
T6865 10266-10268 NN denotes ES
T6866 10269-10273 NN denotes cell
T6867 10281-10286 NNS denotes genes
T6868 10274-10280 NN denotes marker
T6869 10286-10288 , denotes ,
T6870 10288-10292 JJ denotes such
T6871 10293-10295 IN denotes as
T6872 10296-10301 NN denotes Nanog
T6873 10302-10305 CC denotes and
T6874 10306-10310 NN denotes Oct3
T6875 10310-10311 HYPH denotes /
T6876 10311-10312 CD denotes 4
T6877 10312-10314 , denotes ,
T6878 10318-10324 JJ denotes normal
T6879 10325-10327 IN denotes in
T6880 10328-10332 NN denotes ESG1
T6881 10339-10344 NNS denotes cells
T6882 10332-10333 SYM denotes -
T6883 10333-10334 HYPH denotes /
T6884 10334-10335 SYM denotes -
T6885 10336-10338 NN denotes ES
T6886 10344-10345 . denotes .
T6887 10345-10430 sentence denotes We confirmed normal Oct3/4 expression at protein levels by Western blot (Figure 6B).
T6888 10346-10348 PRP denotes We
T6889 10349-10358 VBD denotes confirmed
T6890 10359-10365 JJ denotes normal
T6891 10373-10383 NN denotes expression
T6892 10366-10370 NN denotes Oct3
T6893 10370-10371 HYPH denotes /
T6894 10371-10372 CD denotes 4
T6895 10384-10386 IN denotes at
T6897 10395-10401 NNS denotes levels
T6898 10402-10404 IN denotes by
T6899 10405-10412 NNP denotes Western
T6900 10413-10417 NN denotes blot
T6901 10418-10419 -LRB- denotes (
T6902 10426-10428 NN denotes 6B
T6903 10419-10425 NN denotes Figure
T6904 10428-10429 -RRB- denotes )
T6905 10429-10430 . denotes .
T6906 10430-10527 sentence denotes The overall gene expression profiles were similar between control ES cells and ESG1-/- ES cells.
T6907 10431-10434 DT denotes The
T6908 10459-10467 NNS denotes profiles
T6909 10435-10442 JJ denotes overall
T6910 10443-10447 NN denotes gene
T6911 10448-10458 NN denotes expression
T6912 10468-10472 VBD denotes were
T6913 10473-10480 JJ denotes similar
T6914 10481-10488 IN denotes between
T6915 10489-10496 NN denotes control
T6916 10500-10505 NNS denotes cells
T6917 10497-10499 NN denotes ES
T6918 10506-10509 CC denotes and
T6919 10510-10514 NN denotes ESG1
T6920 10521-10526 NNS denotes cells
T6921 10514-10515 SYM denotes -
T6922 10515-10516 HYPH denotes /
T6923 10516-10517 SYM denotes -
T6924 10518-10520 NN denotes ES
T6925 10526-10527 . denotes .
T6926 10527-10655 sentence denotes Several genes exhibited a greater than two-fold reduction in ESG1-/- cells, including Krt1-8, Pem, Ctgf, Ptgs2, Igf2 and Inhba.
T6927 10528-10535 JJ denotes Several
T6928 10536-10541 NNS denotes genes
T6929 10542-10551 VBD denotes exhibited
T6930 10552-10553 DT denotes a
T6931 10576-10585 NN denotes reduction
T6932 10554-10561 JJR denotes greater
T6933 10567-10575 JJ denotes two-fold
T6934 10562-10566 IN denotes than
T6935 10586-10588 IN denotes in
T6936 10589-10593 NN denotes ESG1
T6937 10597-10602 NNS denotes cells
T6938 10593-10594 SYM denotes -
T6939 10594-10595 HYPH denotes /
T6940 10595-10596 SYM denotes -
T6941 10602-10604 , denotes ,
T6942 10604-10613 VBG denotes including
T6943 10614-10618 NN denotes Krt1
T6944 10618-10619 HYPH denotes -
T6945 10619-10620 CD denotes 8
T6946 10620-10622 , denotes ,
T6947 10622-10625 NN denotes Pem
T6948 10625-10627 , denotes ,
T6949 10627-10631 NN denotes Ctgf
T6950 10631-10633 , denotes ,
T6951 10633-10638 NN denotes Ptgs2
T6952 10638-10640 , denotes ,
T6953 10640-10644 NN denotes Igf2
T6954 10645-10648 CC denotes and
T6955 10649-10654 NN denotes Inhba
T6956 10654-10655 . denotes .
T6957 10655-10718 sentence denotes These genes might be regulated directly or indirectly by ESG1.
T6958 10656-10661 DT denotes These
T6959 10662-10667 NNS denotes genes
T6960 10677-10686 VBN denotes regulated
T6961 10668-10673 MD denotes might
T6962 10674-10676 VB denotes be
T6963 10687-10695 RB denotes directly
T6964 10696-10698 CC denotes or
T6965 10699-10709 RB denotes indirectly
T6966 10710-10712 IN denotes by
T6967 10713-10717 NN denotes ESG1
T6968 10717-10718 . denotes .
T6969 10718-10806 sentence denotes Since ESG1 contains a KH-type RNA-binding domain, it may stabilize mRNA of these genes.
T6970 10719-10724 IN denotes Since
T6971 10730-10738 VBZ denotes contains
T6972 10725-10729 NN denotes ESG1
T6973 10776-10785 VB denotes stabilize
T6974 10739-10740 DT denotes a
T6975 10761-10767 NN denotes domain
T6976 10741-10743 NN denotes KH
T6977 10744-10748 NN denotes type
T6978 10743-10744 HYPH denotes -
T6979 10749-10752 NN denotes RNA
T6980 10753-10760 VBG denotes binding
T6981 10752-10753 HYPH denotes -
T6982 10767-10769 , denotes ,
T6983 10769-10771 PRP denotes it
T6984 10772-10775 MD denotes may
T6985 10786-10790 NN denotes mRNA
T6986 10791-10793 IN denotes of
T6987 10794-10799 DT denotes these
T6988 10800-10805 NNS denotes genes
T6989 10805-10806 . denotes .
T6990 10806-10864 sentence denotes Further studied are required to clarify this possibility.
T6991 10807-10814 RB denotes Further
T6992 10815-10822 VBN denotes studied
T6993 10827-10835 VBN denotes required
T6994 10823-10826 VBP denotes are
T6995 10836-10838 TO denotes to
T6996 10839-10846 VB denotes clarify
T6997 10847-10851 DT denotes this
T6998 10852-10863 NN denotes possibility
T6999 10863-10864 . denotes .
T7000 10864-10865 sentence denotes
T7017 11346-11348 , denotes ,
T7018 11348-11350 PRP denotes we
T7019 11360-11361 NN denotes β
T7020 11366-11370 NN denotes ESG1
T7021 11361-11362 HYPH denotes -
T7022 11362-11365 NN denotes geo
T7023 11365-11366 HYPH denotes -
T7024 11382-11388 NNS denotes clones
T7025 11370-11371 SYM denotes +
T7026 11371-11372 HYPH denotes /
T7027 11372-11373 SYM denotes -
T7028 11374-11376 NN denotes ES
T7029 11377-11381 NN denotes cell
T7030 11389-11393 IN denotes into
T7031 11394-11397 DT denotes the
T7032 11398-11409 NNS denotes blastocysts
T7033 11410-11412 IN denotes of
T7034 11413-11419 NN denotes C57BL6
T7035 11420-11424 NNS denotes mice
T7036 11424-11425 . denotes .
T7037 11425-11478 sentence denotes We obtained germline transmission from three clones.
T7038 11426-11428 PRP denotes We
T7039 11429-11437 VBD denotes obtained
T7040 11438-11446 NN denotes germline
T7041 11447-11459 NN denotes transmission
T7042 11460-11464 IN denotes from
T7043 11465-11470 CD denotes three
T7044 11471-11477 NNS denotes clones
T7045 11477-11478 . denotes .
T7046 11478-11605 sentence denotes We obtained ESG1-/- mice at the Mendelian ratios (36 wild-type, 69 ESG1+/-, and 45 ESG1-/-) from intercrosses of ESG1+/- mice.
T7047 11479-11481 PRP denotes We
T7048 11482-11490 VBD denotes obtained
T7049 11491-11495 NN denotes ESG1
T7050 11499-11503 NNS denotes mice
T7051 11495-11496 SYM denotes -
T7052 11496-11497 HYPH denotes /
T7053 11497-11498 SYM denotes -
T7054 11504-11506 IN denotes at
T7055 11507-11510 DT denotes the
T7056 11521-11527 NNS denotes ratios
T7057 11511-11520 JJ denotes Mendelian
T7058 11528-11529 -LRB- denotes (
T7059 11529-11531 CD denotes 36
T7060 11537-11541 NN denotes type
T7061 11532-11536 JJ denotes wild
T7062 11536-11537 HYPH denotes -
T7063 11541-11543 , denotes ,
T7064 11543-11545 CD denotes 69
T7065 11546-11550 NN denotes ESG1
T7066 11550-11551 SYM denotes +
T7067 11551-11552 HYPH denotes /
T7068 11552-11553 SYM denotes -
T7069 11553-11555 , denotes ,
T7070 11555-11558 CC denotes and
T7071 11559-11561 CD denotes 45
T7072 11562-11566 NN denotes ESG1
T7073 11566-11567 SYM denotes -
T7074 11567-11568 HYPH denotes /
T7075 11568-11569 SYM denotes -
T7076 11569-11570 -RRB- denotes )
T7077 11571-11575 IN denotes from
T7078 11576-11588 NNS denotes intercrosses
T7079 11589-11591 IN denotes of
T7080 11592-11596 NN denotes ESG1
T7081 11600-11604 NNS denotes mice
T7082 11596-11597 SYM denotes +
T7083 11597-11598 HYPH denotes /
T7084 11598-11599 SYM denotes -
T7085 11604-11605 . denotes .
T7086 11605-11694 sentence denotes These animals exhibited normal development, gross appearance, and fertility (not shown).
T7087 11606-11611 DT denotes These
T7088 11612-11619 NNS denotes animals
T7089 11620-11629 VBD denotes exhibited
T7090 11630-11636 JJ denotes normal
T7091 11637-11648 NN denotes development
T7092 11648-11650 , denotes ,
T7093 11650-11655 JJ denotes gross
T7094 11656-11666 NN denotes appearance
T7095 11666-11668 , denotes ,
T7096 11668-11671 CC denotes and
T7097 11672-11681 NN denotes fertility
T7098 11682-11683 -LRB- denotes (
T7099 11687-11692 VBN denotes shown
T7100 11683-11686 RB denotes not
T7101 11692-11693 -RRB- denotes )
T7102 11693-11694 . denotes .
T7103 11694-11789 sentence denotes Histological examination of testis and ovary could not identify any abnormalities (not shown).
T7104 11695-11707 JJ denotes Histological
T7105 11708-11719 NN denotes examination
T7106 11750-11758 VB denotes identify
T7107 11720-11722 IN denotes of
T7108 11723-11729 NN denotes testis
T7109 11730-11733 CC denotes and
T7110 11734-11739 NN denotes ovary
T7111 11740-11745 MD denotes could
T7112 11746-11749 RB denotes not
T7113 11759-11762 DT denotes any
T7114 11763-11776 NNS denotes abnormalities
T7115 11777-11778 -LRB- denotes (
T7116 11782-11787 VBN denotes shown
T7117 11778-11781 RB denotes not
T7118 11787-11788 -RRB- denotes )
T7119 11788-11789 . denotes .
T7120 11789-11890 sentence denotes These data demonstrated that ESG1 is dispensable for both mouse development and germ cell formation.
T7121 11790-11795 DT denotes These
T7122 11796-11800 NNS denotes data
T7123 11801-11813 VBD denotes demonstrated
T7124 11814-11818 IN denotes that
T7125 11824-11826 VBZ denotes is
T7126 11819-11823 NN denotes ESG1
T7127 11827-11838 JJ denotes dispensable
T7128 11839-11842 IN denotes for
T7129 11843-11847 CC denotes both
T7130 11854-11865 NN denotes development
T7131 11848-11853 NN denotes mouse
T7132 11866-11869 CC denotes and
T7133 11870-11874 NN denotes germ
T7134 11875-11879 NN denotes cell
T7135 11880-11889 NN denotes formation
T7136 11889-11890 . denotes .
T7137 11890-11997 sentence denotes We also generated ES cells from blastocysts obtained by intercrosses of ESG1+/- males and ESG1-/- females.
T7138 11891-11893 PRP denotes We
T7139 11899-11908 VBD denotes generated
T7140 11894-11898 RB denotes also
T7141 11909-11911 NN denotes ES
T7142 11912-11917 NNS denotes cells
T7143 11918-11922 IN denotes from
T7144 11923-11934 NNS denotes blastocysts
T7145 11935-11943 VBN denotes obtained
T7146 11944-11946 IN denotes by
T7147 11947-11959 NNS denotes intercrosses
T7148 11960-11962 IN denotes of
T7149 11963-11967 NN denotes ESG1
T7150 11971-11976 NNS denotes males
T7151 11967-11968 SYM denotes +
T7152 11968-11969 HYPH denotes /
T7153 11969-11970 SYM denotes -
T7154 11977-11980 CC denotes and
T7155 11981-11985 NN denotes ESG1
T7156 11989-11996 NNS denotes females
T7157 11985-11986 SYM denotes -
T7158 11986-11987 HYPH denotes /
T7159 11987-11988 SYM denotes -
T7160 11996-11997 . denotes .
T7161 11997-12062 sentence denotes Of the eight ES cell lines established, two clones were ESG1-/-.
T7162 11998-12000 IN denotes Of
T7163 12049-12053 VBD denotes were
T7164 12001-12004 DT denotes the
T7165 12019-12024 NNS denotes lines
T7166 12005-12010 CD denotes eight
T7167 12011-12013 NN denotes ES
T7168 12014-12018 NN denotes cell
T7169 12025-12036 VBN denotes established
T7170 12036-12038 , denotes ,
T7171 12038-12041 CD denotes two
T7172 12042-12048 NNS denotes clones
T7173 12054-12058 NN denotes ESG1
T7174 12058-12059 SYM denotes -
T7175 12059-12060 HYPH denotes /
T7176 12060-12061 SYM denotes -
T7177 12061-12062 . denotes .
T7178 12062-12216 sentence denotes These ESG1-null ES cells demonstrated normal morphology, proliferation, and differentiation (not shown), confirming that ESG1 is dispensable in ES cells.
T7179 12063-12068 DT denotes These
T7180 12082-12087 NNS denotes cells
T7181 12069-12073 NN denotes ESG1
T7182 12074-12078 JJ denotes null
T7183 12073-12074 HYPH denotes -
T7184 12079-12081 NN denotes ES
T7185 12088-12100 VBD denotes demonstrated
T7186 12101-12107 JJ denotes normal
T7187 12108-12118 NN denotes morphology
T7188 12118-12120 , denotes ,
T7189 12120-12133 NN denotes proliferation
T7190 12133-12135 , denotes ,
T7191 12135-12138 CC denotes and
T7192 12139-12154 NN denotes differentiation
T7193 12155-12156 -LRB- denotes (
T7194 12160-12165 VBN denotes shown
T7195 12156-12159 RB denotes not
T7196 12165-12166 -RRB- denotes )
T7197 12166-12168 , denotes ,
T7198 12168-12178 VBG denotes confirming
T7199 12179-12183 IN denotes that
T7200 12189-12191 VBZ denotes is
T7201 12184-12188 NN denotes ESG1
T7202 12192-12203 JJ denotes dispensable
T7203 12204-12206 IN denotes in
T7204 12207-12209 NN denotes ES
T7205 12210-12215 NNS denotes cells
T7206 12215-12216 . denotes .
T7369 12229-12231 TO denotes To
T7370 12232-12239 VB denotes analyze
T7371 12276-12286 VBD denotes identified
T7372 12240-12243 DT denotes the
T7373 12258-12263 NNS denotes roles
T7374 12244-12257 JJ denotes physiological
T7375 12264-12266 IN denotes of
T7376 12267-12271 NN denotes ESG1
T7377 12271-12273 , denotes ,
T7378 12273-12275 PRP denotes we
T7379 12287-12290 DT denotes the
T7380 12297-12301 NN denotes gene
T7381 12291-12296 NN denotes mouse
T7382 12302-12304 IN denotes on
T7383 12305-12315 NN denotes chromosome
T7384 12316-12317 CD denotes 9
T7385 12318-12321 CC denotes and
T7386 12322-12329 VBD denotes deleted
T7387 12330-12332 PRP denotes it
T7388 12333-12335 IN denotes by
T7389 12336-12346 JJ denotes homologous
T7390 12347-12360 NN denotes recombination
T7391 12361-12363 IN denotes in
T7392 12364-12366 NN denotes ES
T7393 12367-12372 NNS denotes cells
T7394 12372-12373 . denotes .
T7395 12373-12571 sentence denotes Despite specific expression in early embryos, germ cells, and pluripotent cells, our data demonstrated that ESG1 is dispensable for mouse development, germ cell formation, and ES cell self-renewal.
T7396 12374-12381 IN denotes Despite
T7397 12464-12476 VBD denotes demonstrated
T7398 12382-12390 JJ denotes specific
T7399 12391-12401 NN denotes expression
T7400 12402-12404 IN denotes in
T7401 12405-12410 JJ denotes early
T7402 12411-12418 NNS denotes embryos
T7403 12418-12420 , denotes ,
T7404 12420-12424 NN denotes germ
T7405 12425-12430 NNS denotes cells
T7406 12430-12432 , denotes ,
T7407 12432-12435 CC denotes and
T7408 12436-12447 JJ denotes pluripotent
T7409 12448-12453 NNS denotes cells
T7410 12453-12455 , denotes ,
T7411 12455-12458 PRP$ denotes our
T7412 12459-12463 NNS denotes data
T7413 12477-12481 IN denotes that
T7414 12487-12489 VBZ denotes is
T7415 12482-12486 NN denotes ESG1
T7416 12490-12501 JJ denotes dispensable
T7417 12502-12505 IN denotes for
T7418 12506-12511 NN denotes mouse
T7419 12512-12523 NN denotes development
T7420 12523-12525 , denotes ,
T7421 12525-12529 NN denotes germ
T7422 12530-12534 NN denotes cell
T7423 12535-12544 NN denotes formation
T7424 12544-12546 , denotes ,
T7425 12546-12549 CC denotes and
T7426 12550-12552 NN denotes ES
T7427 12553-12557 NN denotes cell
T7428 12563-12570 NN denotes renewal
T7429 12558-12562 NN denotes self
T7430 12562-12563 HYPH denotes -
T7431 12570-12571 . denotes .
T8264 12582-12596 NN denotes Identification
T8265 12597-12600 CC denotes and
T8266 12601-12609 NNS denotes analyses
T8267 12610-12612 IN denotes of
T8268 12613-12616 NN denotes BAC
T8269 12617-12623 NNS denotes clones
T8270 12624-12634 VBG denotes containing
T8271 12635-12638 DT denotes the
T8272 12650-12654 NN denotes gene
T8273 12639-12644 NN denotes mouse
T8274 12645-12649 NN denotes ESG1
T8275 12654-12924 sentence denotes To identify bacterial artificial chromosome (BAC) clones containing mouse ESG1 gene, we performed PCR-based screening of mouse BAC library DNA pools (Research Genetics) using the pH34-u38 (5'-GAAGTCTGGTTCCTTGGCAGG-3') and pH34-L394 (5'-ACTCGATACACTGGCCTAGC-3') primers.
T8276 12655-12657 TO denotes To
T8277 12658-12666 VB denotes identify
T8278 12743-12752 VBD denotes performed
T8279 12667-12676 JJ denotes bacterial
T8280 12688-12698 NN denotes chromosome
T8281 12677-12687 JJ denotes artificial
T8282 12705-12711 NNS denotes clones
T8283 12699-12700 -LRB- denotes (
T8284 12700-12703 NN denotes BAC
T8285 12703-12704 -RRB- denotes )
T8286 12712-12722 VBG denotes containing
T8287 12723-12728 NN denotes mouse
T8288 12734-12738 NN denotes gene
T8289 12729-12733 NN denotes ESG1
T8290 12738-12740 , denotes ,
T8291 12740-12742 PRP denotes we
T8292 12753-12756 NN denotes PCR
T8293 12757-12762 VBN denotes based
T8294 12756-12757 HYPH denotes -
T8295 12763-12772 NN denotes screening
T8296 12773-12775 IN denotes of
T8297 12776-12781 NN denotes mouse
T8298 12798-12803 NNS denotes pools
T8299 12782-12785 NN denotes BAC
T8300 12786-12793 NN denotes library
T8301 12794-12797 NN denotes DNA
T8302 12804-12805 -LRB- denotes (
T8303 12814-12822 NNP denotes Genetics
T8304 12805-12813 NNP denotes Research
T8305 12822-12823 -RRB- denotes )
T8306 12824-12829 VBG denotes using
T8307 12830-12833 DT denotes the
T8308 12916-12923 NNS denotes primers
T8309 12834-12838 NN denotes pH34
T8310 12839-12842 NN denotes u38
T8311 12838-12839 HYPH denotes -
T8312 12843-12844 -LRB- denotes (
T8313 12869-12870 CD denotes 3
T8314 12844-12845 CD denotes 5
T8315 12845-12846 SYM denotes '
T8316 12846-12847 HYPH denotes -
T8317 12847-12868 NN denotes GAAGTCTGGTTCCTTGGCAGG
T8318 12868-12869 HYPH denotes -
T8319 12870-12871 SYM denotes '
T8320 12871-12872 -RRB- denotes )
T8321 12873-12876 CC denotes and
T8322 12877-12881 NN denotes pH34
T8323 12882-12886 NN denotes L394
T8324 12881-12882 HYPH denotes -
T8325 12887-12888 -LRB- denotes (
T8326 12912-12913 CD denotes 3
T8327 12888-12889 CD denotes 5
T8328 12889-12890 SYM denotes '
T8329 12890-12891 HYPH denotes -
T8330 12891-12911 NN denotes ACTCGATACACTGGCCTAGC
T8331 12911-12912 HYPH denotes -
T8332 12913-12914 SYM denotes '
T8333 12914-12915 -RRB- denotes )
T8334 12923-12924 . denotes .
T8335 12924-13280 sentence denotes Following restriction enzyme digestion, we performed Southern blot analyses of BAC clones as described [20] using the pH34-U258 (5'-CTCGAGTGTACAGTCAAGTGGTTGCTGGGA-3'), pH34-U65 (5'-GTGACCCTCGTGACCCGTAA-3'), pH34-intron1L (5'-CTGCGTGAGAGAAACACCAAACAGGC-3'), pH34-L545 (5'-TGTGAATGGGAAGGTTACCACTCT-3') and pH34-SCL1 (5'-GCCCTCTTCTGGTTTGTCTCGAAAT-3') probes.
T8336 12925-12934 VBG denotes Following
T8337 12968-12977 VBD denotes performed
T8338 12935-12946 NN denotes restriction
T8339 12947-12953 NN denotes enzyme
T8340 12954-12963 NN denotes digestion
T8341 12963-12965 , denotes ,
T8342 12965-12967 PRP denotes we
T8343 12978-12986 NNP denotes Southern
T8344 12987-12991 NN denotes blot
T8345 12992-13000 NNS denotes analyses
T8346 13001-13003 IN denotes of
T8347 13004-13007 NN denotes BAC
T8348 13008-13014 NNS denotes clones
T8349 13015-13017 IN denotes as
T8350 13018-13027 VBN denotes described
T8351 13028-13029 -LRB- denotes [
T8352 13029-13031 CD denotes 20
T8353 13031-13032 -RRB- denotes ]
T8354 13033-13038 VBG denotes using
T8355 13039-13042 DT denotes the
T8356 13273-13279 NNS denotes probes
T8357 13043-13047 NN denotes pH34
T8358 13048-13052 NN denotes U258
T8359 13047-13048 HYPH denotes -
T8360 13053-13054 -LRB- denotes (
T8361 13057-13087 NN denotes CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
T8362 13054-13055 CD denotes 5
T8363 13055-13056 SYM denotes '
T8364 13056-13057 HYPH denotes -
T8365 13087-13088 HYPH denotes -
T8366 13088-13089 CD denotes 3
T8367 13089-13090 SYM denotes '
T8368 13090-13091 -RRB- denotes )
T8369 13091-13093 , denotes ,
T8370 13093-13097 NN denotes pH34
T8371 13098-13101 NN denotes U65
T8372 13097-13098 HYPH denotes -
T8373 13102-13103 -LRB- denotes (
T8374 13106-13126 NN denotes GTGACCCTCGTGACCCGTAA
T8375 13103-13104 CD denotes 5
T8376 13104-13105 SYM denotes '
T8377 13105-13106 HYPH denotes -
T8378 13126-13127 HYPH denotes -
T8379 13127-13128 CD denotes 3
T8380 13128-13129 SYM denotes '
T8381 13129-13130 -RRB- denotes )
T8382 13130-13132 , denotes ,
T8383 13132-13136 NN denotes pH34
T8384 13137-13145 NN denotes intron1L
T8385 13136-13137 HYPH denotes -
T8386 13146-13147 -LRB- denotes (
T8387 13150-13176 NN denotes CTGCGTGAGAGAAACACCAAACAGGC
T8388 13147-13148 CD denotes 5
T8389 13148-13149 SYM denotes '
T8390 13149-13150 HYPH denotes -
T8391 13176-13177 HYPH denotes -
T8392 13177-13178 CD denotes 3
T8393 13178-13179 SYM denotes '
T8394 13179-13180 -RRB- denotes )
T8395 13180-13182 , denotes ,
T8396 13182-13186 NN denotes pH34
T8397 13187-13191 NN denotes L545
T8398 13186-13187 HYPH denotes -
T8399 13192-13193 -LRB- denotes (
T8400 13196-13220 NN denotes TGTGAATGGGAAGGTTACCACTCT
T8401 13193-13194 CD denotes 5
T8402 13194-13195 SYM denotes '
T8403 13195-13196 HYPH denotes -
T8404 13220-13221 HYPH denotes -
T8405 13221-13222 CD denotes 3
T8406 13222-13223 SYM denotes '
T8407 13223-13224 -RRB- denotes )
T8408 13225-13228 CC denotes and
T8409 13229-13233 NN denotes pH34
T8410 13234-13238 NN denotes SCL1
T8411 13233-13234 HYPH denotes -
T8412 13239-13240 -LRB- denotes (
T8413 13243-13268 NN denotes GCCCTCTTCTGGTTTGTCTCGAAAT
T8414 13240-13241 CD denotes 5
T8415 13241-13242 SYM denotes '
T8416 13242-13243 HYPH denotes -
T8417 13268-13269 HYPH denotes -
T8418 13269-13270 CD denotes 3
T8419 13270-13271 SYM denotes '
T8420 13271-13272 -RRB- denotes )
T8421 13279-13280 . denotes .
T8422 13280-13375 sentence denotes Hybridization with these probes revealed bands containing either the ESG1 gene or pseudogenes.
T8423 13281-13294 NN denotes Hybridization
T8424 13313-13321 VBD denotes revealed
T8425 13295-13299 IN denotes with
T8426 13300-13305 DT denotes these
T8427 13306-13312 NNS denotes probes
T8428 13322-13327 NNS denotes bands
T8429 13328-13338 VBG denotes containing
T8430 13339-13345 CC denotes either
T8431 13355-13359 NN denotes gene
T8432 13346-13349 DT denotes the
T8433 13350-13354 NN denotes ESG1
T8434 13360-13362 CC denotes or
T8435 13363-13374 NNS denotes pseudogenes
T8436 13374-13375 . denotes .
T8437 13375-13537 sentence denotes To sequence the region containing the mouse ESG1 gene and the 3' flanking region, we subcloned a ~15 kbp XhoI-SalI fragment into the pZERO-2 vector (Invitrogen).
T8438 13376-13378 TO denotes To
T8439 13379-13387 VB denotes sequence
T8440 13461-13470 VBD denotes subcloned
T8441 13388-13391 DT denotes the
T8442 13392-13398 NN denotes region
T8443 13399-13409 VBG denotes containing
T8444 13410-13413 DT denotes the
T8445 13425-13429 NN denotes gene
T8446 13414-13419 NN denotes mouse
T8447 13420-13424 NN denotes ESG1
T8448 13430-13433 CC denotes and
T8449 13434-13437 DT denotes the
T8450 13450-13456 NN denotes region
T8451 13438-13439 CD denotes 3
T8452 13439-13440 SYM denotes '
T8453 13441-13449 NN denotes flanking
T8454 13456-13458 , denotes ,
T8455 13458-13460 PRP denotes we
T8456 13471-13472 DT denotes a
T8457 13491-13499 NN denotes fragment
T8458 13473-13474 SYM denotes ~
T8459 13474-13476 CD denotes 15
T8460 13477-13480 NN denotes kbp
T8461 13481-13485 NN denotes XhoI
T8462 13486-13490 NN denotes SalI
T8463 13485-13486 HYPH denotes -
T8464 13500-13504 IN denotes into
T8465 13505-13508 DT denotes the
T8466 13517-13523 NN denotes vector
T8467 13509-13514 NN denotes pZERO
T8468 13514-13515 HYPH denotes -
T8469 13515-13516 CD denotes 2
T8470 13524-13525 -LRB- denotes (
T8471 13525-13535 NNP denotes Invitrogen
T8472 13535-13536 -RRB- denotes )
T8473 13536-13537 . denotes .
T8474 13537-13645 sentence denotes HindIII- or EcoRI-digested fragments of this vector were then cloned into pBluescript KS(-) for sequencing.
T8475 13538-13545 NN denotes HindIII
T8476 13556-13564 VBN denotes digested
T8477 13545-13546 HYPH denotes -
T8478 13547-13549 CC denotes or
T8479 13550-13555 NN denotes EcoRI
T8480 13555-13556 HYPH denotes -
T8481 13565-13574 NNS denotes fragments
T8482 13600-13606 VBN denotes cloned
T8483 13575-13577 IN denotes of
T8484 13578-13582 DT denotes this
T8485 13583-13589 NN denotes vector
T8486 13590-13594 VBD denotes were
T8487 13595-13599 RB denotes then
T8488 13607-13611 IN denotes into
T8489 13612-13623 NN denotes pBluescript
T8490 13624-13626 NN denotes KS
T8491 13626-13627 -LRB- denotes (
T8492 13627-13628 SYM denotes -
T8493 13628-13629 -RRB- denotes )
T8494 13630-13633 IN denotes for
T8495 13634-13644 NN denotes sequencing
T8496 13644-13645 . denotes .
T8497 13645-13768 sentence denotes To sequence the ESG1 pseudogene and the 3' flanking region, an 8 kbp NotI/XhoI fragment was cloned into pBluescript KS(-).
T8498 13646-13648 TO denotes To
T8499 13649-13657 VB denotes sequence
T8500 13738-13744 VBN denotes cloned
T8501 13658-13661 DT denotes the
T8502 13667-13677 NN denotes pseudogene
T8503 13662-13666 NN denotes ESG1
T8504 13678-13681 CC denotes and
T8505 13682-13685 DT denotes the
T8506 13698-13704 NN denotes region
T8507 13686-13687 CD denotes 3
T8508 13687-13688 SYM denotes '
T8509 13689-13697 NN denotes flanking
T8510 13704-13706 , denotes ,
T8511 13706-13708 DT denotes an
T8512 13725-13733 NN denotes fragment
T8513 13709-13710 CD denotes 8
T8514 13711-13714 NN denotes kbp
T8515 13715-13719 NN denotes NotI
T8516 13720-13724 NN denotes XhoI
T8517 13719-13720 HYPH denotes /
T8518 13734-13737 VBD denotes was
T8519 13745-13749 IN denotes into
T8520 13750-13761 NN denotes pBluescript
T8521 13762-13764 NN denotes KS
T8522 13764-13765 -LRB- denotes (
T8523 13765-13766 SYM denotes -
T8524 13766-13767 -RRB- denotes )
T8525 13767-13768 . denotes .
T8526 13768-13850 sentence denotes BamHI- or PstI- fragments of this vector were also cloned into pBluescript KS(-).
T8527 13769-13774 NN denotes BamHI
T8528 13785-13794 NNS denotes fragments
T8529 13774-13775 SYM denotes -
T8530 13776-13778 CC denotes or
T8531 13779-13783 NN denotes PstI
T8532 13783-13784 SYM denotes -
T8533 13820-13826 VBN denotes cloned
T8534 13795-13797 IN denotes of
T8535 13798-13802 DT denotes this
T8536 13803-13809 NN denotes vector
T8537 13810-13814 VBD denotes were
T8538 13815-13819 RB denotes also
T8539 13827-13831 IN denotes into
T8540 13832-13843 NN denotes pBluescript
T8541 13844-13846 NN denotes KS
T8542 13846-13847 -LRB- denotes (
T8543 13847-13848 SYM denotes -
T8544 13848-13849 -RRB- denotes )
T8545 13849-13850 . denotes .
T8546 13850-14075 sentence denotes To identify the sequence containing the 5' flanking regions of the ESG1 gene and the related pseudogenes, we used a TOPO walker kit (Invitrogen) with the pH34-T2L (5'-ACTAGTCGCAGCAGGGATCCAGGAATATCT-3') and pH34-L394 primers.
T8547 13851-13853 TO denotes To
T8548 13854-13862 VB denotes identify
T8549 13960-13964 VBD denotes used
T8550 13863-13866 DT denotes the
T8551 13867-13875 NN denotes sequence
T8552 13876-13886 VBG denotes containing
T8553 13887-13890 DT denotes the
T8554 13903-13910 NNS denotes regions
T8555 13891-13892 CD denotes 5
T8556 13892-13893 SYM denotes '
T8557 13894-13902 NN denotes flanking
T8558 13911-13913 IN denotes of
T8559 13914-13917 DT denotes the
T8560 13923-13927 NN denotes gene
T8561 13918-13922 NN denotes ESG1
T8562 13928-13931 CC denotes and
T8563 13932-13935 DT denotes the
T8564 13944-13955 NNS denotes pseudogenes
T8565 13936-13943 JJ denotes related
T8566 13955-13957 , denotes ,
T8567 13957-13959 PRP denotes we
T8568 13965-13966 DT denotes a
T8569 13979-13982 NN denotes kit
T8570 13967-13971 NN denotes TOPO
T8571 13972-13978 NN denotes walker
T8572 13983-13984 -LRB- denotes (
T8573 13984-13994 NNP denotes Invitrogen
T8574 13994-13995 -RRB- denotes )
T8575 13996-14000 IN denotes with
T8576 14001-14004 DT denotes the
T8577 14067-14074 NNS denotes primers
T8578 14005-14009 NN denotes pH34
T8579 14010-14013 NN denotes T2L
T8580 14009-14010 HYPH denotes -
T8581 14014-14015 -LRB- denotes (
T8582 14049-14050 CD denotes 3
T8583 14015-14016 CD denotes 5
T8584 14016-14017 SYM denotes '
T8585 14017-14018 HYPH denotes -
T8586 14018-14048 NN denotes ACTAGTCGCAGCAGGGATCCAGGAATATCT
T8587 14048-14049 HYPH denotes -
T8588 14050-14051 SYM denotes '
T8589 14051-14052 -RRB- denotes )
T8590 14053-14056 CC denotes and
T8591 14057-14061 NN denotes pH34
T8592 14062-14066 NN denotes L394
T8593 14061-14062 HYPH denotes -
T8594 14074-14075 . denotes .
T8595 14075-14135 sentence denotes The resulting sequence was cloned into pCR2.1 (Invitrogen).
T8596 14076-14079 DT denotes The
T8597 14090-14098 NN denotes sequence
T8598 14080-14089 VBG denotes resulting
T8599 14103-14109 VBN denotes cloned
T8600 14099-14102 VBD denotes was
T8601 14110-14114 IN denotes into
T8602 14115-14121 NN denotes pCR2.1
T8603 14122-14123 -LRB- denotes (
T8604 14123-14133 NNP denotes Invitrogen
T8605 14133-14134 -RRB- denotes )
T8606 14134-14135 . denotes .
T8607 14135-14308 sentence denotes We obtained a ~6 kbp band from the NsiI-digensted library; XbaI-, SpeI-, EcoRI-, and PstI-digested fragments of this band were cloned into pBluescript KS(-) for sequencing.
T8608 14136-14138 PRP denotes We
T8609 14139-14147 VBD denotes obtained
T8610 14263-14269 VBN denotes cloned
T8611 14148-14149 DT denotes a
T8612 14157-14161 NN denotes band
T8613 14150-14151 SYM denotes ~
T8614 14151-14152 CD denotes 6
T8615 14153-14156 NN denotes kbp
T8616 14162-14166 IN denotes from
T8617 14167-14170 DT denotes the
T8618 14186-14193 NN denotes library
T8619 14171-14175 NN denotes NsiI
T8620 14176-14185 VBN denotes digensted
T8621 14175-14176 HYPH denotes -
T8622 14193-14194 : denotes ;
T8623 14195-14199 NN denotes XbaI
T8624 14226-14234 VBN denotes digested
T8625 14199-14200 HYPH denotes -
T8626 14200-14202 , denotes ,
T8627 14202-14206 NN denotes SpeI
T8628 14206-14207 HYPH denotes -
T8629 14207-14209 , denotes ,
T8630 14209-14214 NN denotes EcoRI
T8631 14214-14215 HYPH denotes -
T8632 14215-14217 , denotes ,
T8633 14217-14220 CC denotes and
T8634 14221-14225 NN denotes PstI
T8635 14225-14226 HYPH denotes -
T8636 14235-14244 NNS denotes fragments
T8637 14245-14247 IN denotes of
T8638 14248-14252 DT denotes this
T8639 14253-14257 NN denotes band
T8640 14258-14262 VBD denotes were
T8641 14270-14274 IN denotes into
T8642 14275-14286 NN denotes pBluescript
T8643 14287-14289 NN denotes KS
T8644 14289-14290 -LRB- denotes (
T8645 14290-14291 SYM denotes -
T8646 14291-14292 -RRB- denotes )
T8647 14293-14296 IN denotes for
T8648 14297-14307 NN denotes sequencing
T8649 14307-14308 . denotes .
T8650 14308-14373 sentence denotes This fragment contained the 5' flanking region of the ESG1 gene.
T8651 14309-14313 DT denotes This
T8652 14314-14322 NN denotes fragment
T8653 14323-14332 VBD denotes contained
T8654 14333-14336 DT denotes the
T8655 14349-14355 NN denotes region
T8656 14337-14338 CD denotes 5
T8657 14338-14339 SYM denotes '
T8658 14340-14348 NN denotes flanking
T8659 14356-14358 IN denotes of
T8660 14359-14362 DT denotes the
T8661 14368-14372 NN denotes gene
T8662 14363-14367 NN denotes ESG1
T8663 14372-14373 . denotes .
T8664 14373-14472 sentence denotes A ~3 kbp fragment, obtained from the SacI-digested library, was cloned into pCR2.1 for sequencing.
T8665 14374-14375 DT denotes A
T8666 14383-14391 NN denotes fragment
T8667 14376-14377 SYM denotes ~
T8668 14377-14378 CD denotes 3
T8669 14379-14382 NN denotes kbp
T8670 14438-14444 VBN denotes cloned
T8671 14391-14393 , denotes ,
T8672 14393-14401 VBN denotes obtained
T8673 14402-14406 IN denotes from
T8674 14407-14410 DT denotes the
T8675 14425-14432 NN denotes library
T8676 14411-14415 NN denotes SacI
T8677 14416-14424 VBN denotes digested
T8678 14415-14416 HYPH denotes -
T8679 14432-14434 , denotes ,
T8680 14434-14437 VBD denotes was
T8681 14445-14449 IN denotes into
T8682 14450-14456 NN denotes pCR2.1
T8683 14457-14460 IN denotes for
T8684 14461-14471 NN denotes sequencing
T8685 14471-14472 . denotes .
T8686 14472-14539 sentence denotes This fragment was contained the 5' region flanking the pseudogene.
T8687 14473-14477 DT denotes This
T8688 14478-14486 NN denotes fragment
T8689 14491-14500 VBN denotes contained
T8690 14487-14490 VBD denotes was
T8691 14501-14504 DT denotes the
T8692 14508-14514 NN denotes region
T8693 14505-14506 CD denotes 5
T8694 14506-14507 SYM denotes '
T8695 14515-14523 VBG denotes flanking
T8696 14524-14527 DT denotes the
T8697 14528-14538 NN denotes pseudogene
T8698 14538-14539 . denotes .
T9171 14541-14553 NN denotes Construction
T9172 14554-14556 IN denotes of
T9173 14557-14561 NN denotes ESG1
T9174 14572-14579 NNS denotes vectors
T9175 14562-14571 NN denotes targeting
T9176 14579-14739 sentence denotes We replaced all of the ESG1 exons with two targeting vectors containing either an IRES-β-geo cassette [21] or an IRES-HygR cassette by promoter trap selection.
T9177 14580-14582 PRP denotes We
T9178 14583-14591 VBD denotes replaced
T9179 14592-14595 DT denotes all
T9180 14596-14598 IN denotes of
T9181 14599-14602 DT denotes the
T9182 14608-14613 NNS denotes exons
T9183 14603-14607 NN denotes ESG1
T9184 14614-14618 IN denotes with
T9185 14619-14622 CD denotes two
T9186 14633-14640 NNS denotes vectors
T9187 14623-14632 NN denotes targeting
T9188 14641-14651 VBG denotes containing
T9189 14652-14658 CC denotes either
T9190 14673-14681 NN denotes cassette
T9191 14659-14661 DT denotes an
T9192 14662-14666 NN denotes IRES
T9193 14669-14672 NN denotes geo
T9194 14666-14667 HYPH denotes -
T9195 14667-14668 NN denotes β
T9196 14668-14669 HYPH denotes -
T9197 14682-14683 -LRB- denotes [
T9198 14683-14685 CD denotes 21
T9199 14685-14686 -RRB- denotes ]
T9200 14687-14689 CC denotes or
T9201 14690-14692 DT denotes an
T9202 14698-14702 NN denotes HygR
T9203 14693-14697 NN denotes IRES
T9204 14697-14698 HYPH denotes -
T9205 14703-14711 NN denotes cassette
T9206 14712-14714 IN denotes by
T9207 14715-14723 NN denotes promoter
T9208 14724-14728 NN denotes trap
T9209 14729-14738 NN denotes selection
T9210 14738-14739 . denotes .
T9211 14739-14931 sentence denotes We amplified the 5' arm (1.8 kbp) using KOD plus (TOYOBO) with the pH34-targetpair5-U (5'-CCGCGGAAAGTCAAGAGATTGGGTGG-3') and pH34-targetpair5-L (5'-GCGGCCGCCTTTACGGGTCACGAGGGTCAC-3') primers.
T9212 14740-14742 PRP denotes We
T9213 14743-14752 VBD denotes amplified
T9214 14753-14756 DT denotes the
T9215 14760-14763 NN denotes arm
T9216 14757-14758 CD denotes 5
T9217 14758-14759 SYM denotes '
T9218 14764-14765 -LRB- denotes (
T9219 14769-14772 NN denotes kbp
T9220 14765-14768 CD denotes 1.8
T9221 14772-14773 -RRB- denotes )
T9222 14774-14779 VBG denotes using
T9223 14780-14783 NN denotes KOD
T9224 14784-14788 NN denotes plus
T9225 14789-14790 -LRB- denotes (
T9226 14790-14796 NN denotes TOYOBO
T9227 14796-14797 -RRB- denotes )
T9228 14798-14802 IN denotes with
T9229 14803-14806 DT denotes the
T9230 14923-14930 NNS denotes primers
T9231 14807-14811 NN denotes pH34
T9232 14824-14825 NN denotes U
T9233 14811-14812 HYPH denotes -
T9234 14812-14823 NN denotes targetpair5
T9235 14823-14824 HYPH denotes -
T9236 14826-14827 -LRB- denotes (
T9237 14830-14856 NN denotes CCGCGGAAAGTCAAGAGATTGGGTGG
T9238 14827-14828 CD denotes 5
T9239 14828-14829 SYM denotes '
T9240 14829-14830 HYPH denotes -
T9241 14856-14857 HYPH denotes -
T9242 14857-14858 CD denotes 3
T9243 14858-14859 SYM denotes '
T9244 14859-14860 -RRB- denotes )
T9245 14861-14864 CC denotes and
T9246 14865-14869 NN denotes pH34
T9247 14882-14883 NN denotes L
T9248 14869-14870 HYPH denotes -
T9249 14870-14881 NN denotes targetpair5
T9250 14881-14882 HYPH denotes -
T9251 14884-14885 -LRB- denotes (
T9252 14888-14918 NN denotes GCGGCCGCCTTTACGGGTCACGAGGGTCAC
T9253 14885-14886 CD denotes 5
T9254 14886-14887 SYM denotes '
T9255 14887-14888 HYPH denotes -
T9256 14918-14919 HYPH denotes -
T9257 14919-14920 CD denotes 3
T9258 14920-14921 SYM denotes '
T9259 14921-14922 -RRB- denotes )
T9260 14930-14931 . denotes .
T9261 14931-15097 sentence denotes The 3' arm (5.8 kbp) was amplified using the pH34-targetpair3-U (5'-TGTGGCCAGTGTTTGGTTCTGGCGGG-3') and pH34-targetpair3-L (5'-CTCGAGGACTCGCCATTCTAGCCAAG-3') primers.
T9262 14932-14935 DT denotes The
T9263 14939-14942 NN denotes arm
T9264 14936-14937 CD denotes 3
T9265 14937-14938 SYM denotes '
T9266 14957-14966 VBN denotes amplified
T9267 14943-14944 -LRB- denotes (
T9268 14948-14951 NN denotes kbp
T9269 14944-14947 CD denotes 5.8
T9270 14951-14952 -RRB- denotes )
T9271 14953-14956 VBD denotes was
T9272 14967-14972 VBG denotes using
T9273 14973-14976 DT denotes the
T9274 15089-15096 NNS denotes primers
T9275 14977-14981 NN denotes pH34
T9276 14994-14995 NN denotes U
T9277 14981-14982 HYPH denotes -
T9278 14982-14993 NN denotes targetpair3
T9279 14993-14994 HYPH denotes -
T9280 14996-14997 -LRB- denotes (
T9281 15000-15026 NN denotes TGTGGCCAGTGTTTGGTTCTGGCGGG
T9282 14997-14998 CD denotes 5
T9283 14998-14999 SYM denotes '
T9284 14999-15000 HYPH denotes -
T9285 15026-15027 HYPH denotes -
T9286 15027-15028 CD denotes 3
T9287 15028-15029 SYM denotes '
T9288 15029-15030 -RRB- denotes )
T9289 15031-15034 CC denotes and
T9290 15035-15039 NN denotes pH34
T9291 15052-15053 NN denotes L
T9292 15039-15040 HYPH denotes -
T9293 15040-15051 NN denotes targetpair3
T9294 15051-15052 HYPH denotes -
T9295 15054-15055 -LRB- denotes (
T9296 15058-15084 NN denotes CTCGAGGACTCGCCATTCTAGCCAAG
T9297 15055-15056 CD denotes 5
T9298 15056-15057 SYM denotes '
T9299 15057-15058 HYPH denotes -
T9300 15084-15085 HYPH denotes -
T9301 15085-15086 CD denotes 3
T9302 15086-15087 SYM denotes '
T9303 15087-15088 -RRB- denotes )
T9304 15096-15097 . denotes .
T9305 15097-15182 sentence denotes The IRES β-geo or IRES HygR cassettes were ligated in between the two PCR fragments.
T9306 15098-15101 DT denotes The
T9307 15126-15135 NNS denotes cassettes
T9308 15102-15106 NN denotes IRES
T9309 15109-15112 NN denotes geo
T9310 15107-15108 NN denotes β
T9311 15108-15109 HYPH denotes -
T9312 15113-15115 CC denotes or
T9313 15116-15120 NN denotes IRES
T9314 15121-15125 NN denotes HygR
T9315 15141-15148 VBN denotes ligated
T9316 15136-15140 VBD denotes were
T9317 15149-15151 IN denotes in
T9318 15152-15159 IN denotes between
T9319 15160-15163 DT denotes the
T9320 15172-15181 NNS denotes fragments
T9321 15164-15167 CD denotes two
T9322 15168-15171 NN denotes PCR
T9323 15181-15182 . denotes .
T9324 15182-15251 sentence denotes The diphtheria toxin A cassette was placed downstream of the 3' arm.
T9325 15183-15186 DT denotes The
T9326 15206-15214 NN denotes cassette
T9327 15187-15197 NN denotes diphtheria
T9328 15204-15205 NN denotes A
T9329 15198-15203 NN denotes toxin
T9330 15219-15225 VBN denotes placed
T9331 15215-15218 VBD denotes was
T9332 15226-15236 RB denotes downstream
T9333 15237-15239 IN denotes of
T9334 15240-15243 DT denotes the
T9335 15247-15250 NN denotes arm
T9336 15244-15245 CD denotes 3
T9337 15245-15246 SYM denotes '
T9338 15250-15251 . denotes .
T9339 15251-15390 sentence denotes After linearization with SacII, these targeting vectors were electroporated into 2.0 × 107 RF8 ES cells [22] using a Gene pulser (BIORAD).
T9340 15252-15257 IN denotes After
T9341 15313-15327 VBN denotes electroporated
T9342 15258-15271 NN denotes linearization
T9343 15272-15276 IN denotes with
T9344 15277-15282 NN denotes SacII
T9345 15282-15284 , denotes ,
T9346 15284-15289 DT denotes these
T9347 15300-15307 NNS denotes vectors
T9348 15290-15299 NN denotes targeting
T9349 15308-15312 VBD denotes were
T9350 15328-15332 IN denotes into
T9351 15333-15336 CD denotes 2.0
T9352 15339-15342 CD denotes 107
T9353 15337-15338 SYM denotes ×
T9354 15350-15355 NNS denotes cells
T9355 15343-15346 NN denotes RF8
T9356 15347-15349 NN denotes ES
T9357 15356-15357 -LRB- denotes [
T9358 15357-15359 CD denotes 22
T9359 15359-15360 -RRB- denotes ]
T9360 15361-15366 VBG denotes using
T9361 15367-15368 DT denotes a
T9362 15374-15380 NN denotes pulser
T9363 15369-15373 NN denotes Gene
T9364 15381-15382 -LRB- denotes (
T9365 15382-15388 NNP denotes BIORAD
T9366 15388-15389 -RRB- denotes )
T9367 15389-15390 . denotes .
T9368 15390-15483 sentence denotes Transfected cells were selected with 250 μg/mL G418 or 100 μg/mL hygromycin B, respectively.
T9369 15391-15402 VBN denotes Transfected
T9370 15403-15408 NNS denotes cells
T9371 15414-15422 VBN denotes selected
T9372 15409-15413 VBD denotes were
T9373 15423-15427 IN denotes with
T9374 15428-15431 CD denotes 250
T9375 15432-15434 NN denotes μg
T9376 15438-15442 NN denotes G418
T9377 15434-15435 SYM denotes /
T9378 15435-15437 NN denotes mL
T9379 15443-15445 CC denotes or
T9380 15446-15449 CD denotes 100
T9381 15450-15452 NN denotes μg
T9382 15467-15468 NN denotes B
T9383 15452-15453 SYM denotes /
T9384 15453-15455 NN denotes mL
T9385 15456-15466 NN denotes hygromycin
T9386 15468-15470 , denotes ,
T9387 15470-15482 RB denotes respectively
T9388 15482-15483 . denotes .
T9389 15483-15605 sentence denotes Genomic DNA from G418- or hygromycin B-resistant colonies was screened for homologous recombination by Southern blotting.
T9390 15484-15491 JJ denotes Genomic
T9391 15492-15495 NN denotes DNA
T9392 15546-15554 VBN denotes screened
T9393 15496-15500 IN denotes from
T9394 15501-15505 NN denotes G418
T9395 15523-15532 JJ denotes resistant
T9396 15505-15506 HYPH denotes -
T9397 15507-15509 CC denotes or
T9398 15510-15520 NN denotes hygromycin
T9399 15521-15522 NN denotes B
T9400 15522-15523 HYPH denotes -
T9401 15533-15541 NNS denotes colonies
T9402 15542-15545 VBD denotes was
T9403 15555-15558 IN denotes for
T9404 15559-15569 JJ denotes homologous
T9405 15570-15583 NN denotes recombination
T9406 15584-15586 IN denotes by
T9407 15587-15595 NNP denotes Southern
T9408 15596-15604 NN denotes blotting
T9409 15604-15605 . denotes .
T9832 15607-15615 NNP denotes Southern
T9833 15616-15620 NN denotes blot
T9834 15621-15630 NN denotes screening
T9835 15631-15634 IN denotes for
T9836 15635-15645 JJ denotes homologous
T9837 15646-15659 NN denotes recombination
T9838 15659-15747 sentence denotes ES cells genomic DNA was extracted with PUREGENE™ Cell Lysis Solution (Gentra systems).
T9839 15660-15662 NN denotes ES
T9840 15663-15668 NNS denotes cells
T9841 15677-15680 NN denotes DNA
T9842 15669-15676 JJ denotes genomic
T9843 15685-15694 VBN denotes extracted
T9844 15681-15684 VBD denotes was
T9845 15695-15699 IN denotes with
T9846 15700-15708 NNP denotes PUREGENE
T9847 15721-15729 NN denotes Solution
T9848 15708-15709 SYM denotes ™
T9849 15710-15714 NN denotes Cell
T9850 15715-15720 NN denotes Lysis
T9851 15730-15731 -LRB- denotes (
T9852 15738-15745 NNPS denotes systems
T9853 15731-15737 NNP denotes Gentra
T9854 15745-15746 -RRB- denotes )
T9855 15746-15747 . denotes .
T9856 15747-15913 sentence denotes For 5' Southern blot analysis, genomic DNA was first digested with PstI, then separated on an 0.8% agarose gel and transferred to a nylon membrane as described [20].
T9857 15748-15751 IN denotes For
T9858 15801-15809 VBN denotes digested
T9859 15752-15753 CD denotes 5
T9860 15769-15777 NN denotes analysis
T9861 15753-15754 SYM denotes '
T9862 15755-15763 NNP denotes Southern
T9863 15764-15768 NN denotes blot
T9864 15777-15779 , denotes ,
T9865 15779-15786 JJ denotes genomic
T9866 15787-15790 NN denotes DNA
T9867 15791-15794 VBD denotes was
T9868 15795-15800 RB denotes first
T9869 15810-15814 IN denotes with
T9870 15815-15819 NN denotes PstI
T9871 15819-15821 , denotes ,
T9872 15821-15825 RB denotes then
T9873 15826-15835 VBN denotes separated
T9874 15836-15838 IN denotes on
T9875 15839-15841 DT denotes an
T9876 15855-15858 NN denotes gel
T9877 15842-15845 CD denotes 0.8
T9878 15845-15846 NN denotes %
T9879 15847-15854 NN denotes agarose
T9880 15859-15862 CC denotes and
T9881 15863-15874 VBN denotes transferred
T9882 15875-15877 IN denotes to
T9883 15878-15879 DT denotes a
T9884 15886-15894 NN denotes membrane
T9885 15880-15885 NN denotes nylon
T9886 15895-15897 IN denotes as
T9887 15898-15907 VBN denotes described
T9888 15908-15909 -LRB- denotes [
T9889 15909-15911 CD denotes 20
T9890 15911-15912 -RRB- denotes ]
T9891 15912-15913 . denotes .
T9892 15913-16050 sentence denotes A 560 bp 5' probe was amplified using the ESG1S5 (5'- GATGGTGGTGGTGACTCAGAG -3') and ESG1AS5 as (5'- CCTCCATTGCCTCTATATCAG -3') primers.
T9893 15914-15915 DT denotes A
T9894 15926-15931 NN denotes probe
T9895 15916-15919 CD denotes 560
T9896 15920-15922 NN denotes bp
T9897 15923-15924 CD denotes 5
T9898 15924-15925 SYM denotes '
T9899 15936-15945 VBN denotes amplified
T9900 15932-15935 VBD denotes was
T9901 15946-15951 VBG denotes using
T9902 15952-15955 DT denotes the
T9903 15956-15962 NN denotes ESG1S5
T9904 15963-15964 -LRB- denotes (
T9905 15968-15989 NN denotes GATGGTGGTGGTGACTCAGAG
T9906 15964-15965 CD denotes 5
T9907 15965-15966 SYM denotes '
T9908 15966-15967 HYPH denotes -
T9909 15990-15991 HYPH denotes -
T9910 15991-15992 CD denotes 3
T9911 15992-15993 SYM denotes '
T9912 15993-15994 -RRB- denotes )
T9913 15995-15998 CC denotes and
T9914 15999-16006 NN denotes ESG1AS5
T9915 16007-16009 IN denotes as
T9916 16010-16011 -LRB- denotes (
T9917 16042-16049 NNS denotes primers
T9918 16011-16012 CD denotes 5
T9919 16015-16036 NN denotes CCTCCATTGCCTCTATATCAG
T9920 16012-16013 SYM denotes '
T9921 16013-16014 HYPH denotes -
T9922 16037-16038 HYPH denotes -
T9923 16038-16039 CD denotes 3
T9924 16039-16040 SYM denotes '
T9925 16040-16041 -RRB- denotes )
T9926 16049-16050 . denotes .
T9927 16050-16197 sentence denotes The probe specifically labeled an 18 kbp band from the wild-type locus, a 15 kbp band from the β-geo locus, and a 12 kbp band from the HygR locus.
T9928 16051-16054 DT denotes The
T9929 16055-16060 NN denotes probe
T9930 16074-16081 VBD denotes labeled
T9931 16061-16073 RB denotes specifically
T9932 16082-16084 DT denotes an
T9933 16092-16096 NN denotes band
T9934 16085-16087 CD denotes 18
T9935 16088-16091 NN denotes kbp
T9936 16097-16101 IN denotes from
T9937 16102-16105 DT denotes the
T9938 16116-16121 NN denotes locus
T9939 16106-16110 JJ denotes wild
T9940 16110-16111 HYPH denotes -
T9941 16111-16115 NN denotes type
T9942 16121-16123 , denotes ,
T9943 16123-16124 DT denotes a
T9944 16132-16136 NN denotes band
T9945 16125-16127 CD denotes 15
T9946 16128-16131 NN denotes kbp
T9947 16137-16141 IN denotes from
T9948 16142-16145 DT denotes the
T9949 16152-16157 NN denotes locus
T9950 16146-16147 NN denotes β
T9951 16148-16151 NN denotes geo
T9952 16147-16148 HYPH denotes -
T9953 16157-16159 , denotes ,
T9954 16159-16162 CC denotes and
T9955 16163-16164 DT denotes a
T9956 16172-16176 NN denotes band
T9957 16165-16167 CD denotes 12
T9958 16168-16171 NN denotes kbp
T9959 16177-16181 IN denotes from
T9960 16182-16185 DT denotes the
T9961 16191-16196 NN denotes locus
T9962 16186-16190 NN denotes HygR
T9963 16196-16197 . denotes .
T9964 16197-16268 sentence denotes Genomic DNA was also digested with SpeI for 3' Southern blot analysis.
T9965 16198-16205 JJ denotes Genomic
T9966 16206-16209 NN denotes DNA
T9967 16219-16227 VBN denotes digested
T9968 16210-16213 VBD denotes was
T9969 16214-16218 RB denotes also
T9970 16228-16232 IN denotes with
T9971 16233-16237 NN denotes SpeI
T9972 16238-16241 IN denotes for
T9973 16242-16243 CD denotes 3
T9974 16259-16267 NN denotes analysis
T9975 16243-16244 SYM denotes '
T9976 16245-16253 NNP denotes Southern
T9977 16254-16258 NN denotes blot
T9978 16267-16268 . denotes .
T9979 16268-16415 sentence denotes A 1,010 bp 3' probe was amplified with the pH34U-8000 (5'- CCAACCAGCCAGAGTTTCAGTTAT -3') and pH34L-9000 (5'-GATAAGCTGCTGCCAAAAGACAAG -3') primers.
T9980 16269-16270 DT denotes A
T9981 16283-16288 NN denotes probe
T9982 16271-16276 CD denotes 1,010
T9983 16277-16279 NN denotes bp
T9984 16280-16281 CD denotes 3
T9985 16281-16282 SYM denotes '
T9986 16293-16302 VBN denotes amplified
T9987 16289-16292 VBD denotes was
T9988 16303-16307 IN denotes with
T9989 16308-16311 DT denotes the
T9990 16407-16414 NNS denotes primers
T9991 16312-16317 NN denotes pH34U
T9992 16317-16318 HYPH denotes -
T9993 16318-16322 CD denotes 8000
T9994 16323-16324 -LRB- denotes (
T9995 16328-16352 NN denotes CCAACCAGCCAGAGTTTCAGTTAT
T9996 16324-16325 CD denotes 5
T9997 16325-16326 SYM denotes '
T9998 16326-16327 HYPH denotes -
T9999 16353-16354 HYPH denotes -
T10000 16354-16355 CD denotes 3
T10001 16355-16356 SYM denotes '
T10002 16356-16357 -RRB- denotes )
T10003 16358-16361 CC denotes and
T10004 16362-16367 NN denotes pH34L
T10005 16367-16368 HYPH denotes -
T10006 16368-16372 CD denotes 9000
T10007 16373-16374 -LRB- denotes (
T10008 16377-16401 NN denotes GATAAGCTGCTGCCAAAAGACAAG
T10009 16374-16375 CD denotes 5
T10010 16375-16376 SYM denotes '
T10011 16376-16377 HYPH denotes -
T10012 16402-16403 HYPH denotes -
T10013 16403-16404 CD denotes 3
T10014 16404-16405 SYM denotes '
T10015 16405-16406 -RRB- denotes )
T10016 16414-16415 . denotes .
T10017 16415-16560 sentence denotes The probe hybridized to an 11.5 kbp band from the wild-type locus, a 12.5 kbp band from the β-geo locus, and a 9.5 kbp band from the HygR locus.
T10018 16416-16419 DT denotes The
T10019 16420-16425 NN denotes probe
T10020 16426-16436 VBD denotes hybridized
T10021 16437-16439 IN denotes to
T10022 16440-16442 DT denotes an
T10023 16452-16456 NN denotes band
T10024 16443-16447 CD denotes 11.5
T10025 16448-16451 NN denotes kbp
T10026 16457-16461 IN denotes from
T10027 16462-16465 DT denotes the
T10028 16476-16481 NN denotes locus
T10029 16466-16470 JJ denotes wild
T10030 16471-16475 NN denotes type
T10031 16470-16471 HYPH denotes -
T10032 16481-16483 , denotes ,
T10033 16483-16484 DT denotes a
T10034 16494-16498 NN denotes band
T10035 16485-16489 CD denotes 12.5
T10036 16490-16493 NN denotes kbp
T10037 16499-16503 IN denotes from
T10038 16504-16507 DT denotes the
T10039 16514-16519 NN denotes locus
T10040 16508-16509 NN denotes β
T10041 16510-16513 NN denotes geo
T10042 16509-16510 HYPH denotes -
T10043 16519-16521 , denotes ,
T10044 16521-16524 CC denotes and
T10045 16525-16526 DT denotes a
T10046 16535-16539 NN denotes band
T10047 16527-16530 CD denotes 9.5
T10048 16531-16534 NN denotes kbp
T10049 16540-16544 IN denotes from
T10050 16545-16548 DT denotes the
T10051 16554-16559 NN denotes locus
T10052 16549-16553 NN denotes HygR
T10053 16559-16560 . denotes .
T10414 16562-16572 NN denotes Generation
T10415 16573-16575 IN denotes of
T10416 16576-16585 JJ denotes anti-ESG1
T10417 16597-16607 NNS denotes antibodies
T10418 16586-16596 JJ denotes polyclonal
T10419 16607-16784 sentence denotes The coding sequence of Esg1 was amplified by PCR with the pH34-gw-s (5'- AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA-3') and pH34-gw-as (5'- AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA-3') primers.
T10420 16608-16611 DT denotes The
T10421 16619-16627 NN denotes sequence
T10422 16612-16618 VBG denotes coding
T10423 16640-16649 VBN denotes amplified
T10424 16628-16630 IN denotes of
T10425 16631-16635 NN denotes Esg1
T10426 16636-16639 VBD denotes was
T10427 16650-16652 IN denotes by
T10428 16653-16656 NN denotes PCR
T10429 16657-16661 IN denotes with
T10430 16662-16665 DT denotes the
T10431 16776-16783 NNS denotes primers
T10432 16666-16670 NN denotes pH34
T10433 16674-16675 NN denotes s
T10434 16670-16671 HYPH denotes -
T10435 16671-16673 NN denotes gw
T10436 16673-16674 HYPH denotes -
T10437 16676-16677 -LRB- denotes (
T10438 16681-16714 NN denotes AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
T10439 16677-16678 CD denotes 5
T10440 16678-16679 SYM denotes '
T10441 16679-16680 HYPH denotes -
T10442 16714-16715 HYPH denotes -
T10443 16715-16716 CD denotes 3
T10444 16716-16717 SYM denotes '
T10445 16717-16718 -RRB- denotes )
T10446 16719-16722 CC denotes and
T10447 16723-16727 NN denotes pH34
T10448 16731-16733 NN denotes as
T10449 16727-16728 HYPH denotes -
T10450 16728-16730 NN denotes gw
T10451 16730-16731 HYPH denotes -
T10452 16734-16735 -LRB- denotes (
T10453 16739-16771 NN denotes AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
T10454 16735-16736 CD denotes 5
T10455 16736-16737 SYM denotes '
T10456 16737-16738 HYPH denotes -
T10457 16771-16772 HYPH denotes -
T10458 16772-16773 CD denotes 3
T10459 16773-16774 SYM denotes '
T10460 16774-16775 -RRB- denotes )
T10461 16783-16784 . denotes .
T10462 16784-16877 sentence denotes To construct pDONR-pH34, the resulting PCR product was subcloned into pDONR201 (Invitrogen).
T10463 16785-16787 TO denotes To
T10464 16788-16797 VB denotes construct
T10465 16840-16849 VBN denotes subcloned
T10466 16798-16803 NN denotes pDONR
T10467 16804-16808 NN denotes pH34
T10468 16803-16804 HYPH denotes -
T10469 16808-16810 , denotes ,
T10470 16810-16813 DT denotes the
T10471 16828-16835 NN denotes product
T10472 16814-16823 VBG denotes resulting
T10473 16824-16827 NN denotes PCR
T10474 16836-16839 VBD denotes was
T10475 16850-16854 IN denotes into
T10476 16855-16863 NN denotes pDONR201
T10477 16864-16865 -LRB- denotes (
T10478 16865-16875 NNP denotes Invitrogen
T10479 16875-16876 -RRB- denotes )
T10480 16876-16877 . denotes .
T10481 16877-16950 sentence denotes pDONR-pH34 was interacted with pDEST17 (Invitrogen) by LR recombination.
T10482 16878-16883 NN denotes pDONR
T10483 16884-16888 NN denotes pH34
T10484 16883-16884 HYPH denotes -
T10485 16893-16903 VBN denotes interacted
T10486 16889-16892 VBD denotes was
T10487 16904-16908 IN denotes with
T10488 16909-16916 NN denotes pDEST17
T10489 16917-16918 -LRB- denotes (
T10490 16918-16928 NNP denotes Invitrogen
T10491 16928-16929 -RRB- denotes )
T10492 16930-16932 IN denotes by
T10493 16933-16935 NN denotes LR
T10494 16936-16949 NN denotes recombination
T10495 16949-16950 . denotes .
T10496 16950-17136 sentence denotes After introduction of the resulting expression vector pDEST17-pH34 into BL21-AI E. coli (Invitrogen), recombinant protein production was induced according to the manufacture's protocol.
T10497 16951-16956 IN denotes After
T10498 17088-17095 VBN denotes induced
T10499 16957-16969 NN denotes introduction
T10500 16970-16972 IN denotes of
T10501 16973-16976 DT denotes the
T10502 16998-17004 NN denotes vector
T10503 16977-16986 VBG denotes resulting
T10504 16987-16997 NN denotes expression
T10505 17005-17012 NN denotes pDEST17
T10506 17013-17017 NN denotes pH34
T10507 17012-17013 HYPH denotes -
T10508 17018-17022 IN denotes into
T10509 17023-17027 NN denotes BL21
T10510 17028-17030 NN denotes AI
T10511 17027-17028 HYPH denotes -
T10512 17031-17033 FW denotes E.
T10513 17034-17038 FW denotes coli
T10514 17039-17040 -LRB- denotes (
T10515 17040-17050 NNP denotes Invitrogen
T10516 17050-17051 -RRB- denotes )
T10517 17051-17053 , denotes ,
T10518 17053-17064 JJ denotes recombinant
T10519 17065-17072 NN denotes protein
T10520 17073-17083 NN denotes production
T10521 17084-17087 VBD denotes was
T10522 17096-17105 VBG denotes according
T10523 17106-17108 IN denotes to
T10524 17109-17112 DT denotes the
T10525 17113-17124 NN denotes manufacture
T10526 17127-17135 NN denotes protocol
T10527 17124-17126 POS denotes 's
T10528 17135-17136 . denotes .
T10529 17136-17276 sentence denotes Histidine-tagged ESG1 was purified using Ni-nitrilotriacetic acid agarose (Qiagen) under denaturing conditions in the presence of 8 M urea.
T10530 17137-17146 NN denotes Histidine
T10531 17147-17153 VBN denotes tagged
T10532 17146-17147 HYPH denotes -
T10533 17154-17158 NN denotes ESG1
T10534 17163-17171 VBN denotes purified
T10535 17159-17162 VBD denotes was
T10536 17172-17177 VBG denotes using
T10537 17178-17180 NN denotes Ni
T10538 17203-17210 NN denotes agarose
T10539 17180-17181 : denotes -
T10540 17181-17197 JJ denotes nitrilotriacetic
T10541 17198-17202 NN denotes acid
T10542 17211-17212 -LRB- denotes (
T10543 17212-17218 NNP denotes Qiagen
T10544 17218-17219 -RRB- denotes )
T10545 17220-17225 IN denotes under
T10546 17226-17236 NN denotes denaturing
T10547 17237-17247 NNS denotes conditions
T10548 17248-17250 IN denotes in
T10549 17251-17254 DT denotes the
T10550 17255-17263 NN denotes presence
T10551 17264-17266 IN denotes of
T10552 17267-17268 CD denotes 8
T10553 17269-17270 NN denotes M
T10554 17271-17275 NN denotes urea
T10555 17275-17276 . denotes .
T10556 17276-17424 sentence denotes After dialysis against 6 M urea, the recombinant proteins were injected into New Zealand White rabbits to generate anti-ESG1 polyclonal antibodies.
T10557 17277-17282 IN denotes After
T10558 17340-17348 VBN denotes injected
T10559 17283-17291 NN denotes dialysis
T10560 17292-17299 IN denotes against
T10561 17300-17301 CD denotes 6
T10562 17302-17303 NN denotes M
T10563 17304-17308 NN denotes urea
T10564 17308-17310 , denotes ,
T10565 17310-17313 DT denotes the
T10566 17326-17334 NN denotes proteins
T10567 17314-17325 JJ denotes recombinant
T10568 17335-17339 VBD denotes were
T10569 17349-17353 IN denotes into
T10570 17354-17357 NNP denotes New
T10571 17358-17365 NNP denotes Zealand
T10572 17372-17379 NNS denotes rabbits
T10573 17366-17371 JJ denotes White
T10574 17380-17382 TO denotes to
T10575 17383-17391 VB denotes generate
T10576 17392-17401 JJ denotes anti-ESG1
T10577 17413-17423 NNS denotes antibodies
T10578 17402-17412 JJ denotes polyclonal
T10579 17423-17424 . denotes .
T10856 17426-17433 NNP denotes Western
T10857 17434-17438 NN denotes blot
T10858 17438-17643 sentence denotes After preparation of ES cell extracts with M-Per (Pierce), cellular proteins were separated on sodium dodecyl sulfate (SDS)-14% polyacrylamide gels and transferred to nitrocellulose membranes (Millipore).
T10859 17439-17444 IN denotes After
T10860 17521-17530 VBN denotes separated
T10861 17445-17456 NN denotes preparation
T10862 17457-17459 IN denotes of
T10863 17460-17462 NN denotes ES
T10864 17468-17476 NNS denotes extracts
T10865 17463-17467 NN denotes cell
T10866 17477-17481 IN denotes with
T10867 17482-17483 NN denotes M
T10868 17484-17487 NN denotes Per
T10869 17483-17484 HYPH denotes -
T10870 17488-17489 -LRB- denotes (
T10871 17489-17495 NNP denotes Pierce
T10872 17495-17496 -RRB- denotes )
T10873 17496-17498 , denotes ,
T10874 17498-17506 JJ denotes cellular
T10875 17507-17515 NN denotes proteins
T10876 17516-17520 VBD denotes were
T10877 17531-17533 IN denotes on
T10878 17534-17540 NN denotes sodium
T10879 17549-17556 NN denotes sulfate
T10880 17541-17548 NN denotes dodecyl
T10881 17582-17586 NNS denotes gels
T10882 17557-17558 -LRB- denotes (
T10883 17558-17561 NN denotes SDS
T10884 17561-17562 -RRB- denotes )
T10885 17562-17563 HYPH denotes -
T10886 17563-17565 CD denotes 14
T10887 17565-17566 NN denotes %
T10888 17567-17581 NN denotes polyacrylamide
T10889 17587-17590 CC denotes and
T10890 17591-17602 VBN denotes transferred
T10891 17603-17605 IN denotes to
T10892 17606-17620 NN denotes nitrocellulose
T10893 17621-17630 NNS denotes membranes
T10894 17631-17632 -LRB- denotes (
T10895 17632-17641 NNP denotes Millipore
T10896 17641-17642 -RRB- denotes )
T10897 17642-17643 . denotes .
T10898 17643-17840 sentence denotes Membranes were incubated with anti-ESG1 (1/500 dilution), anti-Oct3/4 (1/500; Santa Cruz Biotechnology), anti-CDK4 (1/200; Santa Cruz Biotechnology), and anti-GFP (1/1000; MBL) primary antibodies.
T10899 17644-17653 NNS denotes Membranes
T10900 17659-17668 VBN denotes incubated
T10901 17654-17658 VBD denotes were
T10902 17669-17673 IN denotes with
T10903 17674-17683 NN denotes anti-ESG1
T10904 17829-17839 NNS denotes antibodies
T10905 17684-17685 -LRB- denotes (
T10906 17691-17699 NN denotes dilution
T10907 17685-17686 CD denotes 1
T10908 17687-17690 CD denotes 500
T10909 17686-17687 SYM denotes /
T10910 17699-17700 -RRB- denotes )
T10911 17700-17702 , denotes ,
T10912 17702-17711 NN denotes anti-Oct3
T10913 17711-17712 HYPH denotes /
T10914 17712-17713 CD denotes 4
T10915 17714-17715 -LRB- denotes (
T10916 17733-17746 NNP denotes Biotechnology
T10917 17715-17716 CD denotes 1
T10918 17717-17720 CD denotes 500
T10919 17716-17717 SYM denotes /
T10920 17720-17721 : denotes ;
T10921 17722-17727 NNP denotes Santa
T10922 17728-17732 NNP denotes Cruz
T10923 17746-17747 -RRB- denotes )
T10924 17747-17749 , denotes ,
T10925 17749-17758 NN denotes anti-CDK4
T10926 17759-17760 -LRB- denotes (
T10927 17778-17791 NNP denotes Biotechnology
T10928 17760-17761 CD denotes 1
T10929 17762-17765 CD denotes 200
T10930 17761-17762 SYM denotes /
T10931 17765-17766 : denotes ;
T10932 17767-17772 NNP denotes Santa
T10933 17773-17777 NNP denotes Cruz
T10934 17791-17792 -RRB- denotes )
T10935 17792-17794 , denotes ,
T10936 17794-17797 CC denotes and
T10937 17798-17806 NN denotes anti-GFP
T10938 17807-17808 -LRB- denotes (
T10939 17816-17819 NN denotes MBL
T10940 17808-17809 CD denotes 1
T10941 17810-17814 CD denotes 1000
T10942 17809-17810 : denotes /
T10943 17814-17815 : denotes ;
T10944 17819-17820 -RRB- denotes )
T10945 17821-17828 JJ denotes primary
T10946 17839-17840 . denotes .
T10947 17840-17980 sentence denotes Horseradish peroxidase-conjugated anti-rabbit and anti-mouse immunoglobulins (1/5000; Cell Signaling) were used to detect antibody binding.
T10948 17841-17852 NN denotes Horseradish
T10949 17853-17863 NN denotes peroxidase
T10950 17864-17874 VBN denotes conjugated
T10951 17863-17864 HYPH denotes -
T10952 17902-17917 NNS denotes immunoglobulins
T10953 17875-17886 JJ denotes anti-rabbit
T10954 17887-17890 CC denotes and
T10955 17891-17901 JJ denotes anti-mouse
T10956 17948-17952 VBN denotes used
T10957 17918-17919 -LRB- denotes (
T10958 17932-17941 NN denotes Signaling
T10959 17919-17920 CD denotes 1
T10960 17921-17925 CD denotes 5000
T10961 17920-17921 SYM denotes /
T10962 17925-17926 : denotes ;
T10963 17927-17931 NN denotes Cell
T10964 17941-17942 -RRB- denotes )
T10965 17943-17947 VBD denotes were
T10966 17953-17955 TO denotes to
T10967 17956-17962 VB denotes detect
T10968 17963-17971 NN denotes antibody
T10969 17972-17979 NN denotes binding
T10970 17979-17980 . denotes .
T10971 17980-18067 sentence denotes We visualized bound antibody with an ECL Western Blotting Detection System (Amersham).
T10972 17981-17983 PRP denotes We
T10973 17984-17994 VBD denotes visualized
T10974 17995-18000 VBN denotes bound
T10975 18001-18009 NN denotes antibody
T10976 18010-18014 IN denotes with
T10977 18015-18017 DT denotes an
T10978 18049-18055 NNP denotes System
T10979 18018-18021 NNP denotes ECL
T10980 18022-18029 NNP denotes Western
T10981 18030-18038 NNP denotes Blotting
T10982 18039-18048 NNP denotes Detection
T10983 18056-18057 -LRB- denotes (
T10984 18057-18065 NNP denotes Amersham
T10985 18065-18066 -RRB- denotes )
T10986 18066-18067 . denotes .
T11476 18069-18079 NN denotes Derivation
T11477 18080-18082 IN denotes of
T11478 18083-18087 NN denotes ESG1
T11479 18088-18097 JJ denotes deficient
T11480 18087-18088 HYPH denotes -
T11481 18101-18106 NNS denotes cells
T11482 18098-18100 NN denotes ES
T11483 18107-18111 IN denotes from
T11484 18112-18123 NNS denotes blastocysts
T11485 18123-18267 sentence denotes Esg1+/-or ESG1-/- mutant female mice were injected with Tamoxifen (10 μg) and Depo-provera (1 mg) subcutaneously on the third day of pregnancy.
T11486 18124-18128 NN denotes Esg1
T11487 18156-18160 NNS denotes mice
T11488 18128-18129 SYM denotes +
T11489 18129-18130 HYPH denotes /
T11490 18130-18131 SYM denotes -
T11491 18131-18133 CC denotes or
T11492 18134-18138 NN denotes ESG1
T11493 18138-18139 SYM denotes -
T11494 18139-18140 HYPH denotes /
T11495 18140-18141 SYM denotes -
T11496 18142-18148 NN denotes mutant
T11497 18149-18155 JJ denotes female
T11498 18166-18174 VBN denotes injected
T11499 18161-18165 VBD denotes were
T11500 18175-18179 IN denotes with
T11501 18180-18189 NN denotes Tamoxifen
T11502 18190-18191 -LRB- denotes (
T11503 18194-18196 NN denotes μg
T11504 18191-18193 CD denotes 10
T11505 18196-18197 -RRB- denotes )
T11506 18198-18201 CC denotes and
T11507 18202-18206 NN denotes Depo
T11508 18207-18214 NN denotes provera
T11509 18206-18207 HYPH denotes -
T11510 18215-18216 -LRB- denotes (
T11511 18218-18220 NN denotes mg
T11512 18216-18217 CD denotes 1
T11513 18220-18221 -RRB- denotes )
T11514 18222-18236 RB denotes subcutaneously
T11515 18237-18239 IN denotes on
T11516 18240-18243 DT denotes the
T11517 18250-18253 NN denotes day
T11518 18244-18249 JJ denotes third
T11519 18254-18256 IN denotes of
T11520 18257-18266 NN denotes pregnancy
T11521 18266-18267 . denotes .
T11522 18267-18651 sentence denotes Four days later, embryos in diapause were flushed out of the uterus and cultured on STO feeder cells in four-well plates in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 20% Fetal Bovine Serum (Hyclone), 0.1 mM Non-Essential Amino Acids (Invitrogen), 2 mM L-glutamine (Invitrogen), 50 U/ml Penicillin-Streptomysin (Invitrogen), and 0.11 mM 2-mercaptoethanol (Invitrogen).
T11523 18268-18272 CD denotes Four
T11524 18273-18277 NNS denotes days
T11525 18278-18283 RB denotes later
T11526 18310-18317 VBN denotes flushed
T11527 18283-18285 , denotes ,
T11528 18285-18292 NNS denotes embryos
T11529 18293-18295 IN denotes in
T11530 18296-18304 NN denotes diapause
T11531 18305-18309 VBD denotes were
T11532 18318-18321 IN denotes out
T11533 18322-18324 IN denotes of
T11534 18325-18328 DT denotes the
T11535 18329-18335 NN denotes uterus
T11536 18336-18339 CC denotes and
T11537 18340-18348 VBN denotes cultured
T11538 18349-18351 IN denotes on
T11539 18352-18355 NN denotes STO
T11540 18363-18368 NNS denotes cells
T11541 18356-18362 NN denotes feeder
T11542 18369-18371 IN denotes in
T11543 18372-18376 CD denotes four
T11544 18377-18381 NN denotes well
T11545 18376-18377 HYPH denotes -
T11546 18382-18388 NNS denotes plates
T11547 18389-18391 IN denotes in
T11548 18392-18400 NNP denotes Dulbecco
T11549 18418-18424 NNP denotes Medium
T11550 18400-18402 POS denotes 's
T11551 18403-18411 VBN denotes Modified
T11552 18412-18417 NNP denotes Eagle
T11553 18425-18426 -LRB- denotes (
T11554 18426-18430 NN denotes DMEM
T11555 18430-18431 -RRB- denotes )
T11556 18432-18444 VBN denotes supplemented
T11557 18445-18449 IN denotes with
T11558 18450-18452 CD denotes 20
T11559 18452-18453 NN denotes %
T11560 18467-18472 NN denotes Serum
T11561 18454-18459 JJ denotes Fetal
T11562 18460-18466 JJ denotes Bovine
T11563 18473-18474 -LRB- denotes (
T11564 18474-18481 NN denotes Hyclone
T11565 18481-18482 -RRB- denotes )
T11566 18482-18484 , denotes ,
T11567 18484-18487 CD denotes 0.1
T11568 18488-18490 NN denotes mM
T11569 18511-18516 NNS denotes Acids
T11570 18491-18504 JJ denotes Non-Essential
T11571 18505-18510 NN denotes Amino
T11572 18517-18518 -LRB- denotes (
T11573 18518-18528 NNP denotes Invitrogen
T11574 18528-18529 -RRB- denotes )
T11575 18529-18531 , denotes ,
T11576 18531-18532 CD denotes 2
T11577 18533-18535 NN denotes mM
T11578 18538-18547 NN denotes glutamine
T11579 18536-18537 NN denotes L
T11580 18537-18538 HYPH denotes -
T11581 18548-18549 -LRB- denotes (
T11582 18549-18559 NNP denotes Invitrogen
T11583 18559-18560 -RRB- denotes )
T11584 18560-18562 , denotes ,
T11585 18562-18564 CD denotes 50
T11586 18565-18566 NN denotes U
T11587 18581-18593 NN denotes Streptomysin
T11588 18566-18567 SYM denotes /
T11589 18567-18569 NN denotes ml
T11590 18570-18580 NN denotes Penicillin
T11591 18580-18581 HYPH denotes -
T11592 18594-18595 -LRB- denotes (
T11593 18595-18605 NNP denotes Invitrogen
T11594 18605-18606 -RRB- denotes )
T11595 18606-18608 , denotes ,
T11596 18608-18611 CC denotes and
T11597 18612-18616 CD denotes 0.11
T11598 18617-18619 NN denotes mM
T11599 18622-18637 NN denotes mercaptoethanol
T11600 18620-18621 CD denotes 2
T11601 18621-18622 HYPH denotes -
T11602 18638-18639 -LRB- denotes (
T11603 18639-18649 NNP denotes Invitrogen
T11604 18649-18650 -RRB- denotes )
T11605 18650-18651 . denotes .
T11606 18651-18781 sentence denotes After six days, the central mass of each explant was harvested, rinsed in PBS, and placed in a drop of trypsin for a few minutes.
T11607 18652-18657 IN denotes After
T11608 18705-18714 VBN denotes harvested
T11609 18658-18661 CD denotes six
T11610 18662-18666 NNS denotes days
T11611 18666-18668 , denotes ,
T11612 18668-18671 DT denotes the
T11613 18680-18684 NN denotes mass
T11614 18672-18679 JJ denotes central
T11615 18685-18687 IN denotes of
T11616 18688-18692 DT denotes each
T11617 18693-18700 NN denotes explant
T11618 18701-18704 VBD denotes was
T11619 18714-18716 , denotes ,
T11620 18716-18722 VBN denotes rinsed
T11621 18723-18725 IN denotes in
T11622 18726-18729 NN denotes PBS
T11623 18729-18731 , denotes ,
T11624 18731-18734 CC denotes and
T11625 18735-18741 VBN denotes placed
T11626 18742-18744 IN denotes in
T11627 18745-18746 DT denotes a
T11628 18747-18751 NN denotes drop
T11629 18752-18754 IN denotes of
T11630 18755-18762 NN denotes trypsin
T11631 18763-18766 IN denotes for
T11632 18767-18768 DT denotes a
T11633 18773-18780 NNS denotes minutes
T11634 18769-18772 JJ denotes few
T11635 18780-18781 . denotes .
T11636 18781-18915 sentence denotes The cell mass was collected with a finely drawn-out Pasteur pipette preloaded with medium, ensuring minimal carryover of the trypsin.
T11637 18782-18785 DT denotes The
T11638 18791-18795 NN denotes mass
T11639 18786-18790 NN denotes cell
T11640 18800-18809 VBN denotes collected
T11641 18796-18799 VBD denotes was
T11642 18810-18814 IN denotes with
T11643 18815-18816 DT denotes a
T11644 18842-18849 NN denotes pipette
T11645 18817-18823 RB denotes finely
T11646 18824-18829 VBN denotes drawn
T11647 18829-18830 HYPH denotes -
T11648 18830-18833 RP denotes out
T11649 18834-18841 NNP denotes Pasteur
T11650 18850-18859 VBN denotes preloaded
T11651 18860-18864 IN denotes with
T11652 18865-18871 NN denotes medium
T11653 18871-18873 , denotes ,
T11654 18873-18881 VBG denotes ensuring
T11655 18882-18889 JJ denotes minimal
T11656 18890-18899 NN denotes carryover
T11657 18900-18902 IN denotes of
T11658 18903-18906 DT denotes the
T11659 18907-18914 NN denotes trypsin
T11660 18914-18915 . denotes .
T11661 18915-18998 sentence denotes The cells were gently transfered into a fresh well with 20% FBS-containing medium.
T11662 18916-18919 DT denotes The
T11663 18920-18925 NNS denotes cells
T11664 18938-18948 VBN denotes transfered
T11665 18926-18930 VBD denotes were
T11666 18931-18937 RB denotes gently
T11667 18949-18953 IN denotes into
T11668 18954-18955 DT denotes a
T11669 18962-18966 NN denotes well
T11670 18956-18961 JJ denotes fresh
T11671 18967-18971 IN denotes with
T11672 18972-18974 CD denotes 20
T11673 18974-18975 NN denotes %
T11674 18991-18997 NN denotes medium
T11675 18976-18979 NN denotes FBS
T11676 18980-18990 VBG denotes containing
T11677 18979-18980 HYPH denotes -
T11678 18997-18998 . denotes .
T11679 18998-19130 sentence denotes The resulting primary ES cell colonies were individually passaged into wells of four-well plates containing STO feeder cell layers.
T11680 18999-19002 DT denotes The
T11681 19029-19037 NNS denotes colonies
T11682 19003-19012 VBG denotes resulting
T11683 19013-19020 JJ denotes primary
T11684 19021-19023 NN denotes ES
T11685 19024-19028 NN denotes cell
T11686 19056-19064 VBN denotes passaged
T11687 19038-19042 VBD denotes were
T11688 19043-19055 RB denotes individually
T11689 19065-19069 IN denotes into
T11690 19070-19075 NNS denotes wells
T11691 19076-19078 IN denotes of
T11692 19079-19083 CD denotes four
T11693 19084-19088 NN denotes well
T11694 19083-19084 HYPH denotes -
T11695 19089-19095 NNS denotes plates
T11696 19096-19106 VBG denotes containing
T11697 19107-19110 NN denotes STO
T11698 19123-19129 NNS denotes layers
T11699 19111-19117 NN denotes feeder
T11700 19118-19122 NN denotes cell
T11701 19129-19130 . denotes .
T11702 19130-19203 sentence denotes Thereafter, cells were expanded by trypsinization of the entire culture.
T11703 19131-19141 RB denotes Thereafter
T11704 19154-19162 VBN denotes expanded
T11705 19141-19143 , denotes ,
T11706 19143-19148 NNS denotes cells
T11707 19149-19153 VBD denotes were
T11708 19163-19165 IN denotes by
T11709 19166-19180 NN denotes trypsinization
T11710 19181-19183 IN denotes of
T11711 19184-19187 DT denotes the
T11712 19195-19202 NN denotes culture
T11713 19188-19194 JJ denotes entire
T11714 19202-19203 . denotes .
T11883 19205-19216 NNS denotes Microarrays
T11884 19216-19315 sentence denotes Total RNA from wild-type ES cells and ESG1-/- ES cells was labeled with Cy3 and Cy5, respectively.
T11885 19217-19222 JJ denotes Total
T11886 19223-19226 NN denotes RNA
T11887 19276-19283 VBN denotes labeled
T11888 19227-19231 IN denotes from
T11889 19232-19236 JJ denotes wild
T11890 19237-19241 NN denotes type
T11891 19236-19237 HYPH denotes -
T11892 19245-19250 NNS denotes cells
T11893 19242-19244 NN denotes ES
T11894 19251-19254 CC denotes and
T11895 19255-19259 NN denotes ESG1
T11896 19266-19271 NNS denotes cells
T11897 19259-19260 SYM denotes -
T11898 19260-19261 HYPH denotes /
T11899 19261-19262 SYM denotes -
T11900 19263-19265 NN denotes ES
T11901 19272-19275 VBD denotes was
T11902 19284-19288 IN denotes with
T11903 19289-19292 NN denotes Cy3
T11904 19293-19296 CC denotes and
T11905 19297-19300 NN denotes Cy5
T11906 19300-19302 , denotes ,
T11907 19302-19314 RB denotes respectively
T11908 19314-19315 . denotes .
T11909 19315-19430 sentence denotes The samples were hybridized to a Mouse Development Microarray (Algilent) according to the manufacturer's protocol.
T11910 19316-19319 DT denotes The
T11911 19320-19327 NNS denotes samples
T11912 19333-19343 VBN denotes hybridized
T11913 19328-19332 VBD denotes were
T11914 19344-19346 IN denotes to
T11915 19347-19348 DT denotes a
T11916 19367-19377 NN denotes Microarray
T11917 19349-19354 NN denotes Mouse
T11918 19355-19366 NN denotes Development
T11919 19378-19379 -LRB- denotes (
T11920 19379-19387 NNP denotes Algilent
T11921 19387-19388 -RRB- denotes )
T11922 19389-19398 VBG denotes according
T11923 19399-19401 IN denotes to
T11924 19402-19405 DT denotes the
T11925 19406-19418 NN denotes manufacturer
T11926 19421-19429 NN denotes protocol
T11927 19418-19420 POS denotes 's
T11928 19429-19430 . denotes .
T11929 19430-19502 sentence denotes Arrays were scanned with a G2565BA Microarray Scanner System (Agilent).
T11930 19431-19437 NNS denotes Arrays
T11931 19443-19450 VBN denotes scanned
T11932 19438-19442 VBD denotes were
T11933 19451-19455 IN denotes with
T11934 19456-19457 DT denotes a
T11935 19485-19491 NN denotes System
T11936 19458-19465 NN denotes G2565BA
T11937 19466-19476 NN denotes Microarray
T11938 19477-19484 NN denotes Scanner
T11939 19492-19493 -LRB- denotes (
T11940 19493-19500 NNP denotes Agilent
T11941 19500-19501 -RRB- denotes )
T11942 19501-19502 . denotes .
T11943 19502-19558 sentence denotes Hybridization was repeated with two independent clones.
T11944 19503-19516 NN denotes Hybridization
T11945 19521-19529 VBN denotes repeated
T11946 19517-19520 VBD denotes was
T11947 19530-19534 IN denotes with
T11948 19535-19538 CD denotes two
T11949 19551-19557 NNS denotes clones
T11950 19539-19550 JJ denotes independent
T11951 19557-19558 . denotes .
T11952 19558-19622 sentence denotes Data were analyzed with GeneSprings software (Silico Genetics).
T11953 19559-19563 NNS denotes Data
T11954 19569-19577 VBN denotes analyzed
T11955 19564-19568 VBD denotes were
T11956 19578-19582 IN denotes with
T11957 19583-19594 NNP denotes GeneSprings
T11958 19595-19603 NN denotes software
T11959 19604-19605 -LRB- denotes (
T11960 19612-19620 NNP denotes Genetics
T11961 19605-19611 NNP denotes Silico
T11962 19620-19621 -RRB- denotes )
T11963 19621-19622 . denotes .
T12055 3250-3265 NNS denotes ESG1pseudogenes
T12056 3266-3276 VBN denotes identified
T12057 3277-3279 IN denotes by
T12058 3280-3281 DT denotes a
T12059 3288-3294 NN denotes search
T12060 3282-3287 NNP denotes Blast
T12061 3295-3297 IN denotes of
T12062 3298-3303 NN denotes mouse
T12063 3312-3321 NNS denotes databases
T12064 3304-3311 JJ denotes genomic
T12065 3321-3322 . denotes .
T12066 3322-3375 sentence denotes Substitution mutations are indicated by black lines.
T12067 3323-3335 NN denotes Substitution
T12068 3336-3345 NNS denotes mutations
T12069 3350-3359 VBN denotes indicated
T12070 3346-3349 VBP denotes are
T12071 3360-3362 IN denotes by
T12072 3363-3368 JJ denotes black
T12073 3369-3374 NNS denotes lines
T12074 3374-3375 . denotes .
T12075 3375-3431 sentence denotes Intron-like gap sequences are indicated with triangles.
T12076 3376-3382 NN denotes Intron
T12077 3383-3387 JJ denotes like
T12078 3382-3383 HYPH denotes -
T12079 3392-3401 NNS denotes sequences
T12080 3388-3391 NN denotes gap
T12081 3406-3415 VBN denotes indicated
T12082 3402-3405 VBP denotes are
T12083 3416-3420 IN denotes with
T12084 3421-3430 NNS denotes triangles
T12085 3430-3431 . denotes .
T12086 3431-3481 sentence denotes Chromosomal localizations are shown on the right.
T12087 3432-3443 JJ denotes Chromosomal
T12088 3444-3457 NNS denotes localizations
T12089 3462-3467 VBN denotes shown
T12090 3458-3461 VBP denotes are
T12091 3468-3470 IN denotes on
T12092 3471-3474 DT denotes the
T12093 3475-3480 NN denotes right
T12094 3480-3481 . denotes .
T12275 4927-4930 NN denotes BAC
T12276 4931-4937 NNS denotes clones
T12277 4938-4948 VBG denotes containing
T12278 4949-4952 DT denotes the
T12279 4958-4962 NN denotes gene
T12280 4953-4957 NN denotes ESG1
T12281 4963-4966 CC denotes and
T12282 4967-4968 DT denotes a
T12283 4981-4991 NN denotes pseudogene
T12284 4969-4980 NN denotes duplication
T12285 4992-4993 -LRB- denotes (
T12286 4993-4995 NN denotes PS
T12287 4995-4996 -RRB- denotes )
T12288 4996-4997 . denotes .
T12289 4997-5049 sentence denotes A) Localization of the gene and PS on chromosome 9.
T12290 4998-4999 LS denotes A
T12291 5001-5013 NN denotes Localization
T12292 4999-5000 -RRB- denotes )
T12293 5014-5016 IN denotes of
T12294 5017-5020 DT denotes the
T12295 5021-5025 NN denotes gene
T12296 5026-5029 CC denotes and
T12297 5030-5032 NN denotes PS
T12298 5033-5035 IN denotes on
T12299 5036-5046 NN denotes chromosome
T12300 5047-5048 CD denotes 9
T12301 5048-5049 . denotes .
T12302 5049-5215 sentence denotes B) Sequence comparison of the gene and PS revealed that these are homologous from eight base pairs upstream of the first exon to 675 bp downstream of the third exon.
T12303 5050-5051 LS denotes B
T12304 5092-5100 VBD denotes revealed
T12305 5051-5052 -RRB- denotes )
T12306 5053-5061 NN denotes Sequence
T12307 5062-5072 NN denotes comparison
T12308 5073-5075 IN denotes of
T12309 5076-5079 DT denotes the
T12310 5080-5084 NN denotes gene
T12311 5085-5088 CC denotes and
T12312 5089-5091 NN denotes PS
T12313 5101-5105 IN denotes that
T12314 5112-5115 VBP denotes are
T12315 5106-5111 DT denotes these
T12316 5116-5126 JJ denotes homologous
T12317 5127-5131 IN denotes from
T12318 5132-5137 CD denotes eight
T12319 5143-5148 NNS denotes pairs
T12320 5138-5142 NN denotes base
T12321 5149-5157 RB denotes upstream
T12322 5158-5160 IN denotes of
T12323 5161-5164 DT denotes the
T12324 5171-5175 NN denotes exon
T12325 5165-5170 JJ denotes first
T12326 5176-5178 IN denotes to
T12327 5179-5182 CD denotes 675
T12328 5183-5185 NN denotes bp
T12329 5186-5196 RB denotes downstream
T12330 5197-5199 IN denotes of
T12331 5200-5203 DT denotes the
T12332 5210-5214 NN denotes exon
T12333 5204-5209 JJ denotes third
T12334 5214-5215 . denotes .
T12335 5215-5268 sentence denotes Substitution mutations are indicated by black lines.
T12336 5216-5228 NN denotes Substitution
T12337 5229-5238 NNS denotes mutations
T12338 5243-5252 VBN denotes indicated
T12339 5239-5242 VBP denotes are
T12340 5253-5255 IN denotes by
T12341 5256-5261 JJ denotes black
T12342 5262-5267 NNS denotes lines
T12343 5267-5268 . denotes .
T12344 5268-5316 sentence denotes The insertion is indicated by an open triangle.
T12345 5269-5272 DT denotes The
T12346 5273-5282 NN denotes insertion
T12347 5286-5295 VBN denotes indicated
T12348 5283-5285 VBZ denotes is
T12349 5296-5298 IN denotes by
T12350 5299-5301 DT denotes an
T12351 5307-5315 NN denotes triangle
T12352 5302-5306 JJ denotes open
T12353 5315-5316 . denotes .
T12543 5327-5335 NN denotes Promoter
T12544 5336-5344 NN denotes enhancer
T12545 5335-5336 HYPH denotes /
T12546 5345-5353 NN denotes activity
T12547 5354-5356 IN denotes of
T12548 5357-5360 DT denotes the
T12549 5366-5370 NN denotes gene
T12550 5361-5365 NN denotes ESG1
T12551 5371-5374 CC denotes and
T12552 5375-5385 NN denotes pseudogene
T12553 5385-5386 . denotes .
T12554 5386-5519 sentence denotes DNA fragments of ~6 kbp isolated from the 5' flanking regions of the gene and PS were transferred into luciferase reporter plasmids.
T12555 5387-5390 NN denotes DNA
T12556 5391-5400 NNS denotes fragments
T12557 5473-5484 VBN denotes transferred
T12558 5401-5403 IN denotes of
T12559 5404-5405 SYM denotes ~
T12560 5405-5406 CD denotes 6
T12561 5407-5410 NN denotes kbp
T12562 5411-5419 VBN denotes isolated
T12563 5420-5424 IN denotes from
T12564 5425-5428 DT denotes the
T12565 5441-5448 NNS denotes regions
T12566 5429-5430 CD denotes 5
T12567 5430-5431 SYM denotes '
T12568 5432-5440 NN denotes flanking
T12569 5449-5451 IN denotes of
T12570 5452-5455 DT denotes the
T12571 5456-5460 NN denotes gene
T12572 5461-5464 CC denotes and
T12573 5465-5467 NN denotes PS
T12574 5468-5472 VBD denotes were
T12575 5485-5489 IN denotes into
T12576 5490-5500 NN denotes luciferase
T12577 5501-5509 NN denotes reporter
T12578 5510-5518 NNS denotes plasmids
T12579 5518-5519 . denotes .
T12580 5519-5681 sentence denotes We introduced the reporter genes into undifferentiated ES cells (open columns), retinoic acid-treated ES cells (grey columns), and NIH3T3 cells (closed columns).
T12581 5520-5522 PRP denotes We
T12582 5523-5533 VBD denotes introduced
T12583 5534-5537 DT denotes the
T12584 5547-5552 NNS denotes genes
T12585 5538-5546 NN denotes reporter
T12586 5553-5557 IN denotes into
T12587 5558-5574 JJ denotes undifferentiated
T12588 5578-5583 NNS denotes cells
T12589 5575-5577 NN denotes ES
T12590 5584-5585 -LRB- denotes (
T12591 5590-5597 NNS denotes columns
T12592 5585-5589 JJ denotes open
T12593 5597-5598 -RRB- denotes )
T12594 5598-5600 , denotes ,
T12595 5600-5608 JJ denotes retinoic
T12596 5609-5613 NN denotes acid
T12597 5614-5621 VBN denotes treated
T12598 5613-5614 HYPH denotes -
T12599 5625-5630 NNS denotes cells
T12600 5622-5624 NN denotes ES
T12601 5631-5632 -LRB- denotes (
T12602 5637-5644 NNS denotes columns
T12603 5632-5636 JJ denotes grey
T12604 5644-5645 -RRB- denotes )
T12605 5645-5647 , denotes ,
T12606 5647-5650 CC denotes and
T12607 5651-5657 NN denotes NIH3T3
T12608 5658-5663 NNS denotes cells
T12609 5664-5665 -LRB- denotes (
T12610 5672-5679 NNS denotes columns
T12611 5665-5671 VBN denotes closed
T12612 5679-5680 -RRB- denotes )
T12613 5680-5681 . denotes .
T12614 5681-5767 sentence denotes Data represent the averages and standard deviations of three independent experiments.
T12615 5682-5686 NNS denotes Data
T12616 5687-5696 VBP denotes represent
T12617 5697-5700 DT denotes the
T12618 5701-5709 NNS denotes averages
T12619 5710-5713 CC denotes and
T12620 5714-5722 JJ denotes standard
T12621 5723-5733 NNS denotes deviations
T12622 5734-5736 IN denotes of
T12623 5737-5742 CD denotes three
T12624 5755-5766 NNS denotes experiments
T12625 5743-5754 JJ denotes independent
T12626 5766-5767 . denotes .
T13078 7550-7558 VBN denotes Targeted
T13079 7559-7569 NN denotes disruption
T13080 7570-7572 IN denotes of
T13081 7573-7576 DT denotes the
T13082 7588-7592 NN denotes gene
T13083 7577-7582 NN denotes mouse
T13084 7583-7587 NN denotes ESG1
T13085 7592-7593 . denotes .
T13086 7593-7616 sentence denotes A) Targeting strategy.
T13087 7594-7595 LS denotes A
T13088 7607-7615 NN denotes strategy
T13089 7595-7596 -RRB- denotes )
T13090 7597-7606 NN denotes Targeting
T13091 7615-7616 . denotes .
T13092 7616-7665 sentence denotes Homologous regions are indicated by thick lines.
T13093 7617-7627 JJ denotes Homologous
T13094 7628-7635 NNS denotes regions
T13095 7640-7649 VBN denotes indicated
T13096 7636-7639 VBP denotes are
T13097 7650-7652 IN denotes by
T13098 7653-7658 JJ denotes thick
T13099 7659-7664 NNS denotes lines
T13100 7664-7665 . denotes .
T13101 7665-7764 sentence denotes Recognition sites of PstI (P) and SpeI (S), which were used for Southern blot analyses, are shown.
T13102 7666-7677 NN denotes Recognition
T13103 7678-7683 NNS denotes sites
T13104 7758-7763 VBN denotes shown
T13105 7684-7686 IN denotes of
T13106 7687-7691 NN denotes PstI
T13107 7692-7693 -LRB- denotes (
T13108 7693-7694 NN denotes P
T13109 7694-7695 -RRB- denotes )
T13110 7696-7699 CC denotes and
T13111 7700-7704 NN denotes SpeI
T13112 7705-7706 -LRB- denotes (
T13113 7706-7707 NN denotes S
T13114 7707-7708 -RRB- denotes )
T13115 7708-7710 , denotes ,
T13116 7710-7715 WDT denotes which
T13117 7721-7725 VBN denotes used
T13118 7716-7720 VBD denotes were
T13119 7726-7729 IN denotes for
T13120 7730-7738 NNP denotes Southern
T13121 7739-7743 NN denotes blot
T13122 7744-7752 NNS denotes analyses
T13123 7752-7754 , denotes ,
T13124 7754-7757 VBP denotes are
T13125 7763-7764 . denotes .
T13126 7764-7893 sentence denotes The gene encoding diphtheria toxin A (DTA) was inserted at the 3' end of the targeting vectors to facilitate negative selection.
T13127 7765-7768 DT denotes The
T13128 7800-7801 NN denotes A
T13129 7769-7773 NN denotes gene
T13130 7774-7782 VBG denotes encoding
T13131 7783-7793 NN denotes diphtheria
T13132 7794-7799 NN denotes toxin
T13133 7812-7820 VBN denotes inserted
T13134 7802-7803 -LRB- denotes (
T13135 7803-7806 NN denotes DTA
T13136 7806-7807 -RRB- denotes )
T13137 7808-7811 VBD denotes was
T13138 7821-7823 IN denotes at
T13139 7824-7827 DT denotes the
T13140 7831-7834 NN denotes end
T13141 7828-7829 CD denotes 3
T13142 7829-7830 SYM denotes '
T13143 7835-7837 IN denotes of
T13144 7838-7841 DT denotes the
T13145 7852-7859 NNS denotes vectors
T13146 7842-7851 NN denotes targeting
T13147 7860-7862 TO denotes to
T13148 7863-7873 VB denotes facilitate
T13149 7874-7882 JJ denotes negative
T13150 7883-7892 NN denotes selection
T13151 7892-7893 . denotes .
T13152 7893-7956 sentence denotes B) Southern blot analyses confirming homologous recombination.
T13153 7894-7895 LS denotes B
T13154 7911-7919 NNS denotes analyses
T13155 7895-7896 -RRB- denotes )
T13156 7897-7905 NNP denotes Southern
T13157 7906-7910 NN denotes blot
T13158 7920-7930 VBG denotes confirming
T13159 7931-7941 JJ denotes homologous
T13160 7942-7955 NN denotes recombination
T13161 7955-7956 . denotes .
T13162 7956-8050 sentence denotes WT, wild-type ES cells; β, β-geo +/- ES cells; H, HygR +/- ES cells; -/-, ESG1-null ES cells.
T13163 7957-7959 NN denotes WT
T13164 7959-7961 , denotes ,
T13165 7961-7965 JJ denotes wild
T13166 7966-7970 NN denotes type
T13167 7965-7966 HYPH denotes -
T13168 7974-7979 NNS denotes cells
T13169 7971-7973 NN denotes ES
T13170 7979-7980 : denotes ;
T13171 7981-7982 NN denotes β
T13172 7982-7984 , denotes ,
T13173 7984-7985 NN denotes β
T13174 7986-7989 NN denotes geo
T13175 7985-7986 HYPH denotes -
T13176 7997-8002 NNS denotes cells
T13177 7990-7991 SYM denotes +
T13178 7991-7992 HYPH denotes /
T13179 7992-7993 SYM denotes -
T13180 7994-7996 NN denotes ES
T13181 8002-8003 : denotes ;
T13182 8004-8005 NN denotes H
T13183 8005-8007 , denotes ,
T13184 8007-8011 NN denotes HygR
T13185 8019-8024 NNS denotes cells
T13186 8012-8013 SYM denotes +
T13187 8013-8014 HYPH denotes /
T13188 8014-8015 SYM denotes -
T13189 8016-8018 NN denotes ES
T13190 8024-8025 : denotes ;
T13191 8026-8027 SYM denotes -
T13192 8028-8029 SYM denotes -
T13193 8027-8028 HYPH denotes /
T13194 8029-8031 , denotes ,
T13195 8031-8035 NN denotes ESG1
T13196 8036-8040 JJ denotes null
T13197 8035-8036 HYPH denotes -
T13198 8044-8049 NNS denotes cells
T13199 8041-8043 NN denotes ES
T13200 8049-8050 . denotes .
T13201 8050-8082 sentence denotes Numbers indicate clone numbers.
T13202 8051-8058 NNS denotes Numbers
T13203 8059-8067 VBP denotes indicate
T13204 8068-8073 NN denotes clone
T13205 8074-8081 NNS denotes numbers
T13206 8081-8082 . denotes .
T13207 8082-8241 sentence denotes C) Northern blot (upper) and western blot (lower) analyses of wild-type ES cells (WT), ESG1-null ES cells (-/-, three clones) and heterozygous ES cells (+/-).
T13208 8083-8084 LS denotes C
T13209 8133-8141 NNS denotes analyses
T13210 8084-8085 -RRB- denotes )
T13211 8086-8094 NNP denotes Northern
T13212 8095-8099 NN denotes blot
T13213 8100-8101 -LRB- denotes (
T13214 8101-8106 JJ denotes upper
T13215 8106-8107 -RRB- denotes )
T13216 8108-8111 CC denotes and
T13217 8112-8119 NNP denotes western
T13218 8120-8124 NN denotes blot
T13219 8125-8126 -LRB- denotes (
T13220 8126-8131 JJ denotes lower
T13221 8131-8132 -RRB- denotes )
T13222 8142-8144 IN denotes of
T13223 8145-8149 JJ denotes wild
T13224 8150-8154 NN denotes type
T13225 8149-8150 HYPH denotes -
T13226 8158-8163 NNS denotes cells
T13227 8155-8157 NN denotes ES
T13228 8164-8165 -LRB- denotes (
T13229 8165-8167 NN denotes WT
T13230 8167-8168 -RRB- denotes )
T13231 8168-8170 , denotes ,
T13232 8170-8174 NN denotes ESG1
T13233 8175-8179 JJ denotes null
T13234 8174-8175 HYPH denotes -
T13235 8183-8188 NNS denotes cells
T13236 8180-8182 NN denotes ES
T13237 8189-8190 -LRB- denotes (
T13238 8201-8207 NNS denotes clones
T13239 8190-8191 SYM denotes -
T13240 8192-8193 SYM denotes -
T13241 8191-8192 HYPH denotes /
T13242 8193-8195 , denotes ,
T13243 8195-8200 CD denotes three
T13244 8207-8208 -RRB- denotes )
T13245 8209-8212 CC denotes and
T13246 8213-8225 JJ denotes heterozygous
T13247 8229-8234 NNS denotes cells
T13248 8226-8228 NN denotes ES
T13249 8235-8236 -LRB- denotes (
T13250 8238-8239 SYM denotes -
T13251 8236-8237 SYM denotes +
T13252 8237-8238 HYPH denotes /
T13253 8239-8240 -RRB- denotes )
T13254 8240-8241 . denotes .
T13255 8241-8299 sentence denotes Northern blot was performed as previously described [20].
T13256 8242-8250 NNP denotes Northern
T13257 8251-8255 NN denotes blot
T13258 8260-8269 VBN denotes performed
T13259 8256-8259 VBD denotes was
T13260 8270-8272 IN denotes as
T13261 8284-8293 VBN denotes described
T13262 8273-8283 RB denotes previously
T13263 8294-8295 -LRB- denotes [
T13264 8295-8297 CD denotes 20
T13265 8297-8298 -RRB- denotes ]
T13266 8298-8299 . denotes .
T13267 8299-8414 sentence denotes To confirm the loading of equal amounts of RNA, ethidium bromide staining of ribosomal RNA is also shown (middle).
T13268 8300-8302 TO denotes To
T13269 8303-8310 VB denotes confirm
T13270 8399-8404 VBN denotes shown
T13271 8311-8314 DT denotes the
T13272 8315-8322 NN denotes loading
T13273 8323-8325 IN denotes of
T13274 8326-8331 JJ denotes equal
T13275 8332-8339 NNS denotes amounts
T13276 8340-8342 IN denotes of
T13277 8343-8346 NN denotes RNA
T13278 8346-8348 , denotes ,
T13279 8348-8356 NN denotes ethidium
T13280 8357-8364 NN denotes bromide
T13281 8365-8373 NN denotes staining
T13282 8374-8376 IN denotes of
T13283 8377-8386 JJ denotes ribosomal
T13284 8387-8390 NN denotes RNA
T13285 8391-8393 VBZ denotes is
T13286 8394-8398 RB denotes also
T13287 8405-8406 -LRB- denotes (
T13288 8406-8412 JJ denotes middle
T13289 8412-8413 -RRB- denotes )
T13290 8413-8414 . denotes .
T13587 9468-9476 NNS denotes Analyses
T13588 9477-9479 IN denotes of
T13589 9480-9484 NN denotes ESG1
T13590 9485-9489 JJ denotes null
T13591 9484-9485 HYPH denotes -
T13592 9493-9498 NNS denotes cells
T13593 9490-9492 NN denotes ES
T13594 9498-9499 . denotes .
T13595 9499-9574 sentence denotes A) The morphology of ESG1-null ES cell colonies grown on STO feeder cells.
T13596 9500-9501 LS denotes A
T13597 9507-9517 NN denotes morphology
T13598 9501-9502 -RRB- denotes )
T13599 9503-9506 DT denotes The
T13600 9518-9520 IN denotes of
T13601 9521-9525 NN denotes ESG1
T13602 9526-9530 JJ denotes null
T13603 9525-9526 HYPH denotes -
T13604 9539-9547 NNS denotes colonies
T13605 9531-9533 NN denotes ES
T13606 9534-9538 NN denotes cell
T13607 9548-9553 VBN denotes grown
T13608 9554-9556 IN denotes on
T13609 9557-9560 NN denotes STO
T13610 9568-9573 NNS denotes cells
T13611 9561-9567 NN denotes feeder
T13612 9573-9574 . denotes .
T13613 9574-9658 sentence denotes B) Growth curve of wild-type (WT), ESG1-null (-/-) and heterozygous (+/-) ES cells.
T13614 9575-9576 LS denotes B
T13615 9585-9590 NN denotes curve
T13616 9576-9577 -RRB- denotes )
T13617 9578-9584 NN denotes Growth
T13618 9591-9593 IN denotes of
T13619 9594-9598 JJ denotes wild
T13620 9599-9603 NN denotes type
T13621 9598-9599 HYPH denotes -
T13622 9652-9657 NNS denotes cells
T13623 9604-9605 -LRB- denotes (
T13624 9605-9607 NN denotes WT
T13625 9607-9608 -RRB- denotes )
T13626 9608-9610 , denotes ,
T13627 9610-9614 NN denotes ESG1
T13628 9614-9615 HYPH denotes -
T13629 9615-9619 JJ denotes null
T13630 9620-9621 -LRB- denotes (
T13631 9621-9622 SYM denotes -
T13632 9623-9624 SYM denotes -
T13633 9622-9623 HYPH denotes /
T13634 9624-9625 -RRB- denotes )
T13635 9626-9629 CC denotes and
T13636 9630-9642 JJ denotes heterozygous
T13637 9643-9644 -LRB- denotes (
T13638 9644-9645 SYM denotes +
T13639 9646-9647 SYM denotes -
T13640 9645-9646 HYPH denotes /
T13641 9647-9648 -RRB- denotes )
T13642 9649-9651 NN denotes ES
T13643 9657-9658 . denotes .
T13644 9658-9720 sentence denotes Each clone (1 × 104 cells/well) was plated in 24-well plates.
T13645 9659-9663 DT denotes Each
T13646 9664-9669 NN denotes clone
T13647 9695-9701 VBN denotes plated
T13648 9670-9671 -LRB- denotes (
T13649 9679-9684 NNS denotes cells
T13650 9671-9672 CD denotes 1
T13651 9675-9678 CD denotes 104
T13652 9673-9674 SYM denotes ×
T13653 9684-9685 SYM denotes /
T13654 9685-9689 NN denotes well
T13655 9689-9690 -RRB- denotes )
T13656 9691-9694 VBD denotes was
T13657 9702-9704 IN denotes in
T13658 9705-9707 CD denotes 24
T13659 9708-9712 NN denotes well
T13660 9707-9708 HYPH denotes -
T13661 9713-9719 NNS denotes plates
T13662 9719-9720 . denotes .
T13663 9720-9793 sentence denotes Cell numbers were determined with a Coulter counter at 2, 4, and 6 days.
T13664 9721-9725 NN denotes Cell
T13665 9726-9733 NNS denotes numbers
T13666 9739-9749 VBN denotes determined
T13667 9734-9738 VBD denotes were
T13668 9750-9754 IN denotes with
T13669 9755-9756 DT denotes a
T13670 9765-9772 NN denotes counter
T13671 9757-9764 NNP denotes Coulter
T13672 9773-9775 IN denotes at
T13673 9776-9777 CD denotes 2
T13674 9788-9792 NNS denotes days
T13675 9777-9779 , denotes ,
T13676 9779-9780 CD denotes 4
T13677 9780-9782 , denotes ,
T13678 9782-9785 CC denotes and
T13679 9786-9787 CD denotes 6
T13680 9792-9793 . denotes .
T13681 9793-9894 sentence denotes Data of +/- and -/- cells are shown as averages and standard deviations of three independent clones.
T13682 9794-9798 NNS denotes Data
T13683 9824-9829 VBN denotes shown
T13684 9799-9801 IN denotes of
T13685 9802-9803 SYM denotes +
T13686 9804-9805 SYM denotes -
T13687 9803-9804 HYPH denotes /
T13688 9814-9819 NNS denotes cells
T13689 9806-9809 CC denotes and
T13690 9810-9811 SYM denotes -
T13691 9812-9813 SYM denotes -
T13692 9811-9812 HYPH denotes /
T13693 9820-9823 VBP denotes are
T13694 9830-9832 IN denotes as
T13695 9833-9841 NNS denotes averages
T13696 9842-9845 CC denotes and
T13697 9846-9854 JJ denotes standard
T13698 9855-9865 NNS denotes deviations
T13699 9866-9868 IN denotes of
T13700 9869-9874 CD denotes three
T13701 9887-9893 NNS denotes clones
T13702 9875-9886 JJ denotes independent
T13703 9893-9894 . denotes .
T13704 9894-9983 sentence denotes C) A section of teratoma derived from ESG1-null ES cells (hematoxylin & eosin staining).
T13705 9895-9896 LS denotes C
T13706 9900-9907 NN denotes section
T13707 9896-9897 -RRB- denotes )
T13708 9898-9899 DT denotes A
T13709 9908-9910 IN denotes of
T13710 9911-9919 NN denotes teratoma
T13711 9920-9927 VBN denotes derived
T13712 9928-9932 IN denotes from
T13713 9933-9937 NN denotes ESG1
T13714 9938-9942 JJ denotes null
T13715 9937-9938 HYPH denotes -
T13716 9946-9951 NNS denotes cells
T13717 9943-9945 NN denotes ES
T13718 9952-9953 -LRB- denotes (
T13719 9973-9981 NN denotes staining
T13720 9953-9964 NN denotes hematoxylin
T13721 9965-9966 CC denotes &
T13722 9967-9972 NN denotes eosin
T13723 9981-9982 -RRB- denotes )
T13724 9982-9983 . denotes .
T13961 10875-10879 NN denotes Gene
T13962 10880-10890 NN denotes expression
T13963 10891-10899 NNS denotes analyses
T13964 10900-10902 IN denotes of
T13965 10903-10907 NN denotes ESG1
T13966 10908-10912 JJ denotes null
T13967 10907-10908 HYPH denotes -
T13968 10916-10921 NNS denotes cells
T13969 10913-10915 NN denotes ES
T13970 10921-10922 . denotes .
T13971 10922-10950 sentence denotes A) DNA microarray analyses.
T13972 10923-10924 LS denotes A
T13973 10941-10949 NNS denotes analyses
T13974 10924-10925 -RRB- denotes )
T13975 10926-10929 NN denotes DNA
T13976 10930-10940 NN denotes microarray
T13977 10949-10950 . denotes .
T13978 10950-11052 sentence denotes Total RNA from wild-type ES cells and ESG1-null ES cells were labeled with Cy3 and Cy5, respectively.
T13979 10951-10956 JJ denotes Total
T13980 10957-10960 NN denotes RNA
T13981 11013-11020 VBN denotes labeled
T13982 10961-10965 IN denotes from
T13983 10966-10970 JJ denotes wild
T13984 10971-10975 NN denotes type
T13985 10970-10971 HYPH denotes -
T13986 10979-10984 NNS denotes cells
T13987 10976-10978 NN denotes ES
T13988 10985-10988 CC denotes and
T13989 10989-10993 NN denotes ESG1
T13990 10994-10998 JJ denotes null
T13991 10993-10994 HYPH denotes -
T13992 11002-11007 NNS denotes cells
T13993 10999-11001 NN denotes ES
T13994 11008-11012 VBD denotes were
T13995 11021-11025 IN denotes with
T13996 11026-11029 NN denotes Cy3
T13997 11030-11033 CC denotes and
T13998 11034-11037 NN denotes Cy5
T13999 11037-11039 , denotes ,
T14000 11039-11051 RB denotes respectively
T14001 11051-11052 . denotes .
T14002 11052-11120 sentence denotes Samples were hybridized to Agilent Mouse Developmental Microarrays.
T14003 11053-11060 NNS denotes Samples
T14004 11066-11076 VBN denotes hybridized
T14005 11061-11065 VBD denotes were
T14006 11077-11079 IN denotes to
T14007 11080-11087 NNP denotes Agilent
T14008 11108-11119 NNS denotes Microarrays
T14009 11088-11093 NN denotes Mouse
T14010 11094-11107 JJ denotes Developmental
T14011 11119-11120 . denotes .
T14012 11120-11170 sentence denotes The averages of two independent clones are shown.
T14013 11121-11124 DT denotes The
T14014 11125-11133 NNS denotes averages
T14015 11164-11169 VBN denotes shown
T14016 11134-11136 IN denotes of
T14017 11137-11140 CD denotes two
T14018 11153-11159 NNS denotes clones
T14019 11141-11152 JJ denotes independent
T14020 11160-11163 VBP denotes are
T14021 11169-11170 . denotes .
T14022 11170-11196 sentence denotes B) Western blot analyses.
T14023 11171-11172 LS denotes B
T14024 11187-11195 NNS denotes analyses
T14025 11172-11173 -RRB- denotes )
T14026 11174-11181 NNP denotes Western
T14027 11182-11186 NN denotes blot
T14028 11195-11196 . denotes .
T14029 11196-11315 sentence denotes Cell lysates from ESG1+/- and ESG1-/- cells were examined for expression of ESG1, Oct3/4 and CDK4 with immunoblotting.
T14030 11197-11201 NN denotes Cell
T14031 11202-11209 NNS denotes lysates
T14032 11246-11254 VBN denotes examined
T14033 11210-11214 IN denotes from
T14034 11215-11219 NN denotes ESG1
T14035 11235-11240 NNS denotes cells
T14036 11219-11220 SYM denotes +
T14037 11220-11221 HYPH denotes /
T14038 11221-11222 SYM denotes -
T14039 11223-11226 CC denotes and
T14040 11227-11231 NN denotes ESG1
T14041 11231-11232 SYM denotes -
T14042 11232-11233 HYPH denotes /
T14043 11233-11234 SYM denotes -
T14044 11241-11245 VBD denotes were
T14045 11255-11258 IN denotes for
T14046 11259-11269 NN denotes expression
T14047 11270-11272 IN denotes of
T14048 11273-11277 NN denotes ESG1
T14049 11277-11279 , denotes ,
T14050 11279-11283 NN denotes Oct3
T14051 11283-11284 HYPH denotes /
T14052 11284-11285 CD denotes 4
T14053 11286-11289 CC denotes and
T14054 11290-11294 NN denotes CDK4
T14055 11295-11299 IN denotes with
T14056 11300-11314 NN denotes immunoblotting
T14057 11314-11315 . denotes .
T4103 2823-2825 IN denotes in
T4104 2826-2829 NN denotes DNA
T4105 2830-2839 NNS denotes fragments
T4106 2840-2843 IN denotes for
T4107 2879-2887 VBD denotes remained
T4108 2844-2849 WDT denotes which
T4109 2850-2853 DT denotes the
T4110 2866-2878 NN denotes localization
T4111 2854-2865 JJ denotes chromosomal
T4112 2888-2896 JJ denotes unmapped
T4113 2896-2897 . denotes .
T4114 2897-3037 sentence denotes While these pseudodgenes have significant homology to ESG1 cDNA, they could not produce functional proteins, because of critical mutations.
T4115 2898-2903 IN denotes While
T4116 2923-2927 VBP denotes have
T4117 2904-2909 DT denotes these
T4118 2910-2922 NNS denotes pseudodgenes
T4119 2978-2985 VB denotes produce
T4120 2928-2939 JJ denotes significant
T4121 2940-2948 NN denotes homology
T4183 3528-3535 JJ denotes similar
T4184 3536-3538 IN denotes to
T4185 3539-3542 DT denotes the
T4186 3548-3552 NN denotes gene
T4386 4581-4582 . denotes .
T4387 4582-4767 sentence denotes The 5' flanking region of this DNA fragment exhibited strong promoter/enhancer activity by luciferase reporter assays in undifferentiated ES cells, but not in somatic cells (Figure 3).
T4388 4583-4586 DT denotes The
T4389 4599-4605 NN denotes region
T4390 4587-4588 CD denotes 5
T4391 4588-4589 SYM denotes '
T4392 4590-4598 NN denotes flanking
T4393 4627-4636 VBD denotes exhibited
T4394 4606-4608 IN denotes of
T4477 5888-5889 DT denotes a
T4478 5908-5916 NN denotes sequence
T4479 5890-5892 CD denotes 68
T4480 5893-5896 NN denotes kbp
T4481 5897-5907 JJ denotes intergenic
T4482 5916-5918 , denotes ,
T4483 5918-5922 VBD denotes were
T4484 5932-5934 IN denotes in
T4485 5935-5943 JJ denotes opposite
T4486 5944-5954 NNS denotes directions
T6502 7231-7233 NN denotes 4A
T6503 7224-7230 NN denotes Figure
T6504 7233-7234 -RRB- denotes )
T6505 7235-7245 VBN denotes introduced
T6506 7246-7250 IN denotes into
T6507 7251-7254 NN denotes RF8
T6508 7258-7263 NNS denotes cells
T6509 7255-7257 NN denotes ES
T6510 7264-7266 IN denotes by
T6511 7267-7282 NN denotes electroporation
T6512 7282-7283 . denotes .
T6513 7283-7444 sentence denotes We obtained eight ES cell clones with correct homologous recombination of the β-geo targeting vector, which was confirmed by Southern blot analysis (Figure 4B).
T6514 7284-7286 PRP denotes We
T6515 7287-7295 VBD denotes obtained
T6516 7296-7301 CD denotes eight
T6573 8427-8429 sentence denotes mo
T6574 8427-8428 VBG denotes m
T6575 8428-8429 JJ denotes o
T6576 8429-8436 sentence denotes zygous
T6607 8524-8528 NN denotes G418
T6608 8529-8538 JJ denotes resistant
T6609 8528-8529 HYPH denotes -
T6610 8548-8554 VBN denotes tested
T6729 9100-9105 IN denotes after
T6730 9106-9109 DT denotes the
T6731 9110-9117 NN denotes removal
T6732 9118-9120 IN denotes of
T6733 9121-9129 NN denotes leukemia
T6734 9141-9147 NN denotes factor
T6735 9130-9140 JJ denotes inhibitory
T6736 9148-9149 -LRB- denotes (
T6737 9156-9158 NN denotes 5B
T6798 9984-9988 sentence denotes We e
T6799 9987-9988 NNS denotes e
T6800 9984-9985 NN denotes W
T6801 9985-9986 JJ denotes e
T6802 9988-9990 sentence denotes xa
T6810 10005-10015 NN denotes expression
T6811 10025-10027 IN denotes of
T6812 10028-10032 NN denotes ESG1
T6813 10039-10044 NNS denotes cells
T6814 10032-10033 SYM denotes -
T7006 11322-11323 NN denotes e
T7007 11325-11328 sentence denotes te
T7008 11325-11326 CD denotes t
T7009 11326-11327 JJ denotes e
T7010 11328-11333 sentence denotes ESG1-
T7011 11328-11332 NN denotes ESG1
T7012 11332-11333 SYM denotes -
T7013 11333-11425 sentence denotes knockout mice, we injected β-geo-ESG1+/- ES cell clones into the blastocysts of C57BL6 mice.
T7014 11351-11359 VBD denotes injected
T7015 11333-11341 NN denotes knockout
T4095 2766-2768 IN denotes In
T4096 2812-2822 VBN denotes identified
T4097 2769-2777 NN denotes addition
T4098 2777-2779 , denotes ,
T4099 2779-2782 CD denotes two
T4100 2795-2806 NNS denotes pseudogenes
T4101 2783-2794 JJ denotes intron-less
T4102 2807-2811 VBD denotes were
T4173 3482-3588 sentence denotes On chromosome 9, we identified a DNA fragment similar to the ESG1 gene that included two putative introns.
T4174 3482-3484 IN denotes On
T4175 3502-3512 VBD denotes identified
T4176 3485-3495 NN denotes chromosome
T4177 3496-3497 CD denotes 9
T4178 3497-3499 , denotes ,
T4179 3499-3501 PRP denotes we
T4180 3513-3514 DT denotes a
T4181 3519-3527 NN denotes fragment
T4182 3515-3518 NN denotes DNA
T4370 4522-4524 NN denotes AG
T4371 4524-4526 , denotes ,
T4372 4526-4536 VBG denotes fulfilling
T4373 4537-4540 DT denotes the
T4374 4547-4551 NN denotes rule
T4375 4541-4543 NN denotes GT
T4376 4544-4546 NN denotes AG
T4377 4543-4544 HYPH denotes -
T4378 4552-4554 IN denotes of
T4379 4555-4559 NN denotes exon
T4380 4560-4566 NN denotes intron
T4381 4559-4560 HYPH denotes -
T4382 4567-4576 NNS denotes junctions
T4383 4577-4578 -LRB- denotes [
T4384 4578-4580 CD denotes 19
T4385 4580-4581 -RRB- denotes ]
T4449 4916-5855 sentence denotes Figure 2 BAC clones containing the ESG1 gene and a duplication pseudogene (PS). A) Localization of the gene and PS on chromosome 9. B) Sequence comparison of the gene and PS revealed that these are homologous from eight base pairs upstream of the first exon to 675 bp downstream of the third exon. Substitution mutations are indicated by black lines. The insertion is indicated by an open triangle. Figure 3 Promoter/enhancer activity of the ESG1 gene and pseudogene. DNA fragments of ~6 kbp isolated from the 5' flanking regions of the gene and PS were transferred into luciferase reporter plasmids. We introduced the reporter genes into undifferentiated ES cells (open columns), retinoic acid-treated ES cells (grey columns), and NIH3T3 cells (closed columns). Data represent the averages and standard deviations of three independent experiments. We also found that the two BAC clones contained another ESG1-like sequence (Figure 2A).
T4450 5768-5770 PRP denotes We
T4451 5776-5781 VBD denotes found
T4452 5771-5775 RB denotes also
T4453 5782-5786 IN denotes that
T4454 5806-5815 VBD denotes contained
T4455 5787-5790 DT denotes the
T4456 5799-5805 NNS denotes clones
T4457 5791-5794 CD denotes two
T4458 5795-5798 NN denotes BAC
T4459 5816-5823 DT denotes another
T4460 5834-5842 NN denotes sequence
T4461 5824-5828 NN denotes ESG1
T4462 5829-5833 JJ denotes like
T4463 5828-5829 HYPH denotes -
T4464 5843-5844 -LRB- denotes (
T4465 5851-5853 NN denotes 2A
T4466 5844-5850 NN denotes Figure
T4467 5853-5854 -RRB- denotes )
T4468 5854-5855 . denotes .
T4469 5855-5955 sentence denotes The two sequences, separated by a 68 kbp intergenic sequence, were oriented in opposite directions.
T4470 5856-5859 DT denotes The
T4471 5864-5873 NNS denotes sequences
T4472 5860-5863 CD denotes two
T4473 5923-5931 VBN denotes oriented
T4474 5873-5875 , denotes ,
T4475 5875-5884 VBN denotes separated
T4476 5885-5887 IN denotes by
T6492 7162-7172 NN denotes hygromycin
T6493 7173-7182 JJ denotes resistant
T6494 7188-7189 -LRB- denotes (
T6495 7189-7193 NN denotes HygR
T6496 7193-7194 -RRB- denotes )
T6497 7195-7200 VBG denotes using
T6498 7201-7204 CD denotes two
T6499 7215-7222 NNS denotes vectors
T6500 7205-7214 NN denotes targeting
T6501 7223-7224 -LRB- denotes (
T6571 8422-8423 WDT denotes i
T6572 8423-8424 NNP denotes n
T6577 8429-8435 JJ denotes zygous
T6578 8436-8438 sentence denotes mu
T6579 8436-8437 VBN denotes m
T6580 8437-8438 RB denotes u
T6581 8438-8443 sentence denotes tant
T6582 8438-8439 DT denotes t
T6583 8439-8441 NN denotes an
T6584 8441-8442 NNS denotes t
T6585 8443-8516 sentence denotes ES cells, we introduced the β-geo vector into HygR heterozygous ES cells.
T6586 8456-8466 VBD denotes introduced
T6587 8446-8451 NNS denotes cells
T6588 8443-8445 NN denotes ES
T6589 8451-8453 , denotes ,
T6590 8453-8455 PRP denotes we
T6591 8467-8470 DT denotes the
T6592 8477-8483 NN denotes vector
T6593 8471-8472 NN denotes β
T6594 8473-8476 NN denotes geo
T6595 8472-8473 HYPH denotes -
T6596 8484-8488 IN denotes into
T6597 8489-8493 NN denotes HygR
T6598 8494-8506 JJ denotes heterozygous
T6599 8510-8515 NNS denotes cells
T6600 8507-8509 NN denotes ES
T6601 8515-8516 . denotes .
T6602 8516-8593 sentence denotes Of 105 G418-resistant colonies tested, 49 were homozygous for ESG1 deletion.
T6603 8517-8519 IN denotes Of
T6604 8559-8563 VBD denotes were
T6605 8520-8523 CD denotes 105
T6606 8539-8547 NNS denotes colonies
T6716 9049-9050 -LRB- denotes (
T6717 9057-9059 NN denotes 5B
T6718 9050-9056 NN denotes Figure
T6719 9059-9060 -RRB- denotes )
T6720 9060-9061 . denotes .
T6721 9061-9199 sentence denotes ESG1-/- cells differentiated normally after the removal of leukemia inhibitory factor (Figure 5B) or retinoic acid treatment (not shown).
T6722 9062-9066 NN denotes ESG1
T6723 9070-9075 NNS denotes cells
T6724 9066-9067 SYM denotes -
T6725 9067-9068 HYPH denotes /
T6726 9068-9069 SYM denotes -
T6727 9076-9090 VBD denotes differentiated
T6728 9091-9099 RB denotes normally
T6803 9989-9990 NN denotes a
T6804 9988-9989 NN denotes x
T6805 9990-10116 sentence denotes mined the gene expression profiles of ESG1-/- ES cells using oligonucleotide-based DNA microarrays representing ~20,000 genes.
T6806 9990-9995 VBD denotes mined
T6807 9996-9999 DT denotes the
T6808 10016-10024 NNS denotes profiles
T6809 10000-10004 NN denotes gene
T6896 10387-10394 NN denotes protein
T7001 11316-11319 sentence denotes To
T7002 11316-11318 JJ denotes To
T7003 11319-11325 sentence denotes genera
T7004 11319-11322 NNP denotes gen
T7005 11323-11325 NNS denotes ra
T7016 11342-11346 NNS denotes mice
R325 T2208 T2209 amod Embryonic,stem
R326 T2209 T2210 nmod stem,cells
R327 T2210 T2214 nsubjpass cells,derived
R328 T2211 T2209 punct (,stem
R329 T2212 T2209 appos ES,stem
R330 T2213 T2210 punct ),cells
R331 T2215 T2214 auxpass were,derived
R332 T2216 T2214 advmod first,derived
R333 T2217 T2214 prep from,derived
R334 T2218 T2219 det the,blastocysts
R335 T2219 T2217 pobj blastocysts,from
R336 T2220 T2219 prep of,blastocysts
R337 T2221 T2220 pobj mice,of
R338 T2222 T2214 prep in,derived
R339 T2223 T2222 pobj 1981,in
R340 T2224 T2225 punct [,2
R341 T2225 T2223 parataxis 2,1981
R342 T2226 T2225 nummod 1,2
R343 T2227 T2225 punct ",",2
R344 T2228 T2225 punct ],2
R345 T2229 T2214 cc and,derived
R346 T2230 T2214 conj humans,derived
R347 T2231 T2230 prep in,humans
R348 T2232 T2231 pobj 1998,in
R349 T2233 T2234 punct [,3
R350 T2234 T2232 parataxis 3,1998
R351 T2235 T2234 punct ],3
R352 T2236 T2214 punct .,derived
R353 T2238 T2239 compound ES,cells
R354 T2239 T2240 nsubj cells,have
R355 T2241 T2242 nummod two,properties
R356 T2242 T2240 dobj properties,have
R357 T2243 T2242 amod important,properties
R358 T2244 T2242 punct : ,properties
R359 T2245 T2242 appos theability,properties
R360 T2246 T2247 aux to,maintain
R361 T2247 T2245 acl maintain,theability
R362 T2248 T2247 dobj pluripotency,maintain
R363 T2249 T2248 punct ", ",pluripotency
R364 T2250 T2251 dep which,is
R365 T2251 T2248 relcl is,pluripotency
R366 T2252 T2253 det the,ability
R367 T2253 T2251 attr ability,is
R368 T2254 T2255 aux to,differentiate
R369 T2255 T2253 acl differentiate,ability
R370 T2256 T2255 prep into,differentiate
R371 T2257 T2258 det a,variety
R372 T2258 T2256 pobj variety,into
R373 T2259 T2258 amod wide,variety
R374 T2260 T2258 prep of,variety
R375 T2261 T2260 pobj cells,of
R376 T2262 T2245 punct ", ",theability
R377 T2263 T2245 cc and,theability
R378 T2264 T2265 amod rapid,proliferation
R379 T2265 T2245 conj proliferation,theability
R380 T2266 T2240 punct .,have
R381 T2268 T2269 det These,characteristics
R382 T2269 T2270 nsubj characteristics,make
R383 T2271 T2272 compound mouse,cells
R384 T2272 T2274 nsubj cells,component
R385 T2273 T2272 compound ES,cells
R386 T2274 T2270 ccomp component,make
R387 T2275 T2274 det an,component
R388 T2276 T2274 amod essential,component
R389 T2277 T2274 prep of,component
R390 T2278 T2279 compound gene,targeting
R391 T2279 T2280 compound targeting,technology
R392 T2280 T2277 pobj technology,of
R393 T2281 T2270 punct .,make
R394 T2283 T2284 det These,qualities
R395 T2284 T2285 nsubj qualities,make
R396 T2286 T2285 advmod also,make
R397 T2287 T2288 amod human,cells
R398 T2288 T2290 nsubj cells,sources
R399 T2289 T2288 compound ES,cells
R400 T2290 T2285 ccomp sources,make
R401 T2291 T2290 amod attractive,sources
R402 T2292 T2290 prep for,sources
R403 T2293 T2294 compound cell,transplantation
R404 T2294 T2295 compound transplantation,therapy
R405 T2295 T2292 pobj therapy,for
R406 T2296 T2297 aux to,treat
R407 T2297 T2295 advcl treat,therapy
R408 T2298 T2299 amod various,diseases
R409 T2299 T2297 dobj diseases,treat
R410 T2300 T2299 punct ", ",diseases
R411 T2301 T2299 prep including,diseases
R412 T2302 T2303 amod spinal,cord
R413 T2303 T2304 compound cord,injuries
R414 T2304 T2301 pobj injuries,including
R415 T2305 T2304 cc and,injuries
R416 T2306 T2307 amod juvenile,diabetes
R417 T2307 T2304 conj diabetes,injuries
R418 T2308 T2285 punct .,make
R419 T2310 T2311 det The,mechanisms
R420 T2311 T2313 nsubj mechanisms,are
R421 T2312 T2311 amod molecular,mechanisms
R422 T2314 T2311 acl underlying,mechanisms
R423 T2315 T2316 det the,pluripotency
R424 T2316 T2314 dobj pluripotency,underlying
R425 T2317 T2316 cc and,pluripotency
R426 T2318 T2319 amod rapid,proliferation
R427 T2319 T2316 conj proliferation,pluripotency
R428 T2320 T2316 prep of,pluripotency
R429 T2321 T2322 compound ES,cells
R430 T2322 T2320 pobj cells,of
R431 T2323 T2313 advmod currently,are
R432 T2324 T2325 det a,focus
R433 T2325 T2313 attr focus,are
R434 T2326 T2325 amod major,focus
R435 T2327 T2325 prep of,focus
R436 T2328 T2329 det the,field
R437 T2329 T2327 pobj field,of
R438 T2330 T2329 prep of,field
R439 T2331 T2332 compound stem,cell
R440 T2332 T2333 compound cell,biology
R441 T2333 T2330 pobj biology,of
R442 T2334 T2335 punct [,4
R443 T2335 T2313 parataxis 4,are
R444 T2336 T2337 punct -,6
R445 T2337 T2335 prep 6,4
R446 T2338 T2335 punct ],4
R447 T2339 T2313 punct .,are
R448 T2341 T2342 aux To,identify
R449 T2342 T2343 advcl identify,utilized
R450 T2344 T2342 dobj molecules,identify
R451 T2345 T2344 relcl essential,molecules
R452 T2346 T2345 prep in,essential
R453 T2347 T2348 compound ES,cells
R454 T2348 T2346 pobj cells,in
R455 T2349 T2345 prep for,essential
R456 T2350 T2351 det these,properties
R457 T2351 T2349 pobj properties,for
R458 T2352 T2343 punct ", ",utilized
R459 T2353 T2354 amod several,groups
R460 T2354 T2343 nsubj groups,utilized
R461 T2355 T2343 aux have,utilized
R462 T2356 T2357 compound transcriptome,analyses
R463 T2357 T2343 dobj analyses,utilized
R464 T2358 T2359 aux to,identify
R465 T2359 T2343 advcl identify,utilized
R466 T2360 T2359 dobj genes,identify
R467 T2361 T2362 advmod specifically,expressed
R468 T2362 T2360 acl expressed,genes
R469 T2363 T2362 prep in,expressed
R470 T2364 T2365 compound ES,cells
R471 T2365 T2363 pobj cells,in
R472 T2366 T2365 cc and,cells
R473 T2367 T2368 amod early,embryos
R474 T2368 T2365 conj embryos,cells
R475 T2369 T2343 punct .,utilized
R476 T2371 T2372 det These,analyses
R477 T2372 T2373 nsubj analyses,identified
R478 T2374 T2372 punct ", ",analyses
R479 T2375 T2372 prep including,analyses
R480 T2376 T2377 compound DNA,analyses
R481 T2377 T2375 pobj analyses,including
R482 T2378 T2377 compound microarray,analyses
R483 T2379 T2380 punct [,7
R484 T2380 T2377 parataxis 7,analyses
R485 T2381 T2380 punct ],7
R486 T2382 T2377 cc and,analyses
R487 T2383 T2384 amod expressed,sequence
R488 T2384 T2385 compound sequence,analyses
R489 T2385 T2377 conj analyses,analyses
R490 T2386 T2385 compound tag,analyses
R491 T2387 T2388 punct [,8
R492 T2388 T2385 parataxis 8,analyses
R493 T2389 T2390 punct -,12
R494 T2390 T2388 prep 12,8
R495 T2391 T2388 punct ],8
R496 T2392 T2373 punct ", ",identified
R497 T2393 T2394 amod several,transcripts
R498 T2394 T2373 dobj transcripts,identified
R499 T2395 T2394 amod common,transcripts
R500 T2396 T2394 punct ", ",transcripts
R501 T2397 T2394 prep including,transcripts
R502 T2398 T2397 pobj ESG1,including
R503 T2399 T2400 dep that,designated
R504 T2400 T2394 relcl designated,transcripts
R505 T2401 T2400 auxpass was,designated
R506 T2402 T2400 advmod also,designated
R507 T2403 T2400 oprd dppa5,designated
R508 T2404 T2403 cc or,dppa5
R509 T2405 T2403 conj ECAT2,dppa5
R510 T2406 T2373 punct .,identified
R511 T2408 T2409 nsubjpass ESG1,identified
R512 T2410 T2409 auxpass was,identified
R513 T2411 T2409 advmod originally,identified
R514 T2412 T2409 prep as,identified
R515 T2413 T2414 det a,Ph34
R516 T2414 T2412 pobj Ph34,as
R517 T2415 T2414 compound transcript,Ph34
R518 T2416 T2417 dep that,regulated
R519 T2417 T2414 relcl regulated,Ph34
R520 T2418 T2417 auxpass was,regulated
R521 T2419 T2417 advmod down,regulated
R522 T2420 T2417 punct -,regulated
R523 T2421 T2417 agent by,regulated
R524 T2422 T2423 amod retinoic,acid
R525 T2423 T2421 pobj acid,by
R526 T2424 T2417 prep in,regulated
R527 T2425 T2426 amod embryonic,carcinoma
R528 T2426 T2427 compound carcinoma,cells
R529 T2427 T2424 pobj cells,in
R530 T2428 T2429 punct [,13
R531 T2429 T2417 parataxis 13,regulated
R532 T2430 T2429 punct ],13
R533 T2431 T2409 punct .,identified
R534 T2433 T2434 det The,expression
R535 T2434 T2435 nsubjpass expression,confined
R536 T2436 T2434 prep of,expression
R537 T2437 T2438 det this,gene
R538 T2438 T2436 pobj gene,of
R539 T2439 T2435 auxpass was,confined
R540 T2440 T2435 prep in,confined
R541 T2441 T2440 pobj mice,in
R542 T2442 T2435 prep to,confined
R543 T2443 T2444 amod early,embryos
R544 T2444 T2442 pobj embryos,to
R545 T2445 T2444 cc and,embryos
R546 T2446 T2447 compound germ,cells
R547 T2447 T2444 conj cells,embryos
R548 T2448 T2449 punct [,14
R549 T2449 T2435 parataxis 14,confined
R550 T2450 T2449 punct ],14
R551 T2451 T2435 punct .,confined
R552 T2453 T2454 nsubjpass It,expressed
R553 T2455 T2454 auxpass is,expressed
R554 T2456 T2454 advmod also,expressed
R555 T2457 T2454 prep in,expressed
R556 T2458 T2459 amod pluripotent,cells
R557 T2459 T2457 pobj cells,in
R558 T2460 T2459 punct ", ",cells
R559 T2461 T2459 prep including,cells
R560 T2462 T2463 compound ES,cells
R561 T2463 T2461 pobj cells,including
R562 T2464 T2463 punct ", ",cells
R563 T2465 T2466 amod embryonic,cells
R564 T2466 T2463 conj cells,cells
R565 T2467 T2466 compound germ,cells
R566 T2468 T2466 punct ", ",cells
R567 T2469 T2466 cc and,cells
R568 T2470 T2471 amod multipotent,cells
R569 T2471 T2466 conj cells,cells
R570 T2472 T2471 compound germline,cells
R571 T2473 T2471 compound stem,cells
R572 T2474 T2475 punct [,15
R573 T2475 T2454 parataxis 15,expressed
R574 T2476 T2475 punct ],15
R575 T2477 T2454 punct .,expressed
R576 T2479 T2480 nsubj ESG1,encodes
R577 T2481 T2482 det a,polypeptide
R578 T2482 T2480 dobj polypeptide,encodes
R579 T2483 T2482 prep of,polypeptide
R580 T2484 T2485 nummod 118,acids
R581 T2485 T2483 pobj acids,of
R582 T2486 T2485 compound amino,acids
R583 T2487 T2488 dep that,contains
R584 T2488 T2482 relcl contains,polypeptide
R585 T2489 T2490 det a,domain
R586 T2490 T2488 dobj domain,contains
R587 T2491 T2490 amod single,domain
R588 T2492 T2490 compound KH,domain
R589 T2493 T2490 punct ", ",domain
R590 T2494 T2495 dep which,is
R591 T2495 T2490 relcl is,domain
R592 T2496 T2497 det an,domain
R593 T2497 T2495 attr domain,is
R594 T2498 T2499 npadvmod RNA,binding
R595 T2499 T2497 amod binding,domain
R596 T2500 T2499 punct -,binding
R597 T2501 T2502 punct [,16
R598 T2502 T2495 parataxis 16,is
R599 T2503 T2502 punct ],16
R600 T2504 T2480 punct .,encodes
R601 T2506 T2507 nsubj It,remains
R602 T2508 T2507 acomp unclear,remains
R603 T2509 T2507 punct ", ",remains
R604 T2510 T2507 advmod however,remains
R605 T2511 T2507 punct ", ",remains
R606 T2512 T2513 mark if,functions
R607 T2513 T2507 ccomp functions,remains
R608 T2514 T2513 nsubj ESG1,functions
R609 T2515 T2513 prep as,functions
R610 T2516 T2517 det an,protein
R611 T2517 T2515 pobj protein,as
R612 T2518 T2519 npadvmod RNA,binding
R613 T2519 T2517 amod binding,protein
R614 T2520 T2519 punct -,binding
R615 T2521 T2513 cc or,functions
R616 T2522 T2523 det the,roles
R617 T2523 T2513 conj roles,functions
R618 T2524 T2525 nsubj it,plays
R619 T2525 T2523 advcl plays,roles
R620 T2526 T2525 prep in,plays
R621 T2527 T2528 compound ES,cells
R622 T2528 T2526 pobj cells,in
R623 T2529 T2528 cc and,cells
R624 T2530 T2528 conj mice,cells
R625 T2531 T2507 punct .,remains
R626 T2533 T2534 amod Previous,screening
R627 T2535 T2536 amod genomic,library
R628 T2536 T2534 compound library,screening
R629 T2537 T2534 prep by,screening
R630 T2538 T2539 amod identified,clones
R631 T2539 T2537 pobj clones,by
R632 T2540 T2539 amod genomic,clones
R633 T2541 T2539 acl containing,clones
R634 T2542 T2543 det the,gene
R635 T2543 T2541 dobj gene,containing
R636 T2544 T2543 compound mouse,gene
R637 T2545 T2543 compound ESG1,gene
R638 T2546 T2543 cc and,gene
R639 T2547 T2548 nummod seven,pseudogenes
R640 T2548 T2543 conj pseudogenes,gene
R641 T2549 T2550 punct [,17
R642 T2550 T2541 parataxis 17,containing
R643 T2551 T2550 punct ],17
R644 T2552 T2534 punct .,screening
R645 T2554 T2555 nsubj Two,exhibit
R646 T2556 T2554 prep of,Two
R647 T2557 T2558 det these,pseudogenes
R648 T2558 T2556 pobj pseudogenes,of
R649 T2559 T2560 det a,structure
R650 T2560 T2555 dobj structure,exhibit
R651 T2561 T2560 amod similar,structure
R652 T2562 T2563 compound exon,intron
R653 T2563 T2560 compound intron,structure
R654 T2564 T2563 punct -,intron
R655 T2565 T2560 prep as,structure
R656 T2566 T2567 det the,gene
R657 T2567 T2565 pobj gene,as
R658 T2568 T2567 compound ESG1,gene
R659 T2569 T2555 punct ", ",exhibit
R660 T2570 T2555 advcl indicating,exhibit
R661 T2571 T2572 poss their,generation
R662 T2572 T2570 dobj generation,indicating
R663 T2573 T2570 prep by,indicating
R664 T2574 T2575 compound gene,duplication
R665 T2575 T2573 pobj duplication,by
R666 T2576 T2555 punct .,exhibit
R667 T2578 T2579 det The,pseudogenes
R668 T2579 T2582 nsubj pseudogenes,contain
R669 T2580 T2579 nummod five,pseudogenes
R670 T2581 T2579 amod remaining,pseudogenes
R671 T2583 T2582 aux did,contain
R672 T2584 T2582 neg not,contain
R673 T2585 T2586 det any,introns
R674 T2586 T2582 dobj introns,contain
R675 T2587 T2582 punct ", ",contain
R676 T2588 T2582 advcl indicating,contain
R677 T2589 T2590 mark that,generated
R678 T2590 T2588 ccomp generated,indicating
R679 T2591 T2590 nsubjpass these,generated
R680 T2592 T2590 auxpass were,generated
R681 T2593 T2590 agent by,generated
R682 T2594 T2593 pobj retrotransposition,by
R683 T2595 T2594 prep of,retrotransposition
R684 T2596 T2597 compound ESG1,transcripts
R685 T2597 T2595 pobj transcripts,of
R686 T2598 T2582 punct .,contain
R687 T2600 T2601 det The,localizations
R688 T2601 T2603 nsubjpass localizations,reported
R689 T2602 T2601 amod chromosomal,localizations
R690 T2604 T2601 prep of,localizations
R691 T2605 T2606 det the,gene
R692 T2606 T2604 pobj gene,of
R693 T2607 T2606 compound mouse,gene
R694 T2608 T2606 compound ESG1,gene
R695 T2609 T2606 cc and,gene
R696 T2610 T2606 conj pseudogenes,gene
R697 T2611 T2603 punct ", ",reported
R698 T2612 T2603 advmod however,reported
R699 T2613 T2603 punct ", ",reported
R700 T2614 T2603 aux have,reported
R701 T2615 T2603 neg not,reported
R702 T2616 T2603 auxpass been,reported
R703 T2617 T2603 punct .,reported
R704 T2619 T2620 prep In,determined
R705 T2621 T2622 det this,study
R706 T2622 T2619 pobj study,In
R707 T2623 T2620 punct ", ",determined
R708 T2624 T2620 nsubj we,determined
R709 T2625 T2626 det the,structure
R710 T2626 T2620 dobj structure,determined
R711 T2627 T2626 prep of,structure
R712 T2628 T2629 det the,gene
R713 T2629 T2627 pobj gene,of
R714 T2630 T2629 compound mouse,gene
R715 T2631 T2629 acl encoding,gene
R716 T2632 T2633 det this,protein
R717 T2633 T2631 dobj protein,encoding
R718 T2634 T2633 cc and,protein
R719 T2635 T2636 amod related,pseudogenes
R720 T2636 T2633 conj pseudogenes,protein
R721 T2637 T2620 punct .,determined
R722 T2639 T2640 nsubj We,performed
R723 T2641 T2640 advmod also,performed
R724 T2642 T2643 compound gene,targeting
R725 T2643 T2640 dobj targeting,performed
R726 T2644 T2645 aux to,determine
R727 T2645 T2640 advcl determine,performed
R728 T2646 T2647 det the,function
R729 T2647 T2645 dobj function,determine
R730 T2648 T2647 amod physiological,function
R731 T2649 T2647 prep of,function
R732 T2650 T2649 pobj ESG1,of
R733 T2651 T2640 punct .,performed
R734 T4020 T4021 amod Chromosomal,localization
R735 T4022 T4021 cc and,localization
R736 T4023 T4021 conj structures,localization
R737 T4024 T4021 prep of,localization
R738 T4025 T4026 compound mouse,gene
R739 T4026 T4024 pobj gene,of
R740 T4027 T4026 compound ESG1,gene
R741 T4028 T4026 cc and,gene
R742 T4029 T4026 conj psedogenes,gene
R743 T4031 T4032 aux To,determine
R744 T4032 T4033 advcl determine,performed
R745 T4034 T4035 det the,localizations
R746 T4035 T4032 dobj localizations,determine
R747 T4036 T4035 amod chromosomal,localizations
R748 T4037 T4035 prep of,localizations
R749 T4038 T4039 det the,gene
R750 T4039 T4037 pobj gene,of
R751 T4040 T4039 compound mouse,gene
R752 T4041 T4039 compound ESG1,gene
R753 T4042 T4039 cc and,gene
R754 T4043 T4039 conj pseudogenes,gene
R755 T4044 T4033 punct ", ",performed
R756 T4045 T4033 nsubj we,performed
R757 T4046 T4047 det a,analysis
R758 T4047 T4033 dobj analysis,performed
R759 T4048 T4047 compound Blast,analysis
R760 T4049 T4047 prep of,analysis
R761 T4050 T4051 det the,database
R762 T4051 T4049 pobj database,of
R763 T4052 T4051 nmod mouse,database
R764 T4053 T4051 amod genomic,database
R765 T4054 T4033 prep with,performed
R766 T4055 T4056 det the,sequence
R767 T4056 T4054 pobj sequence,with
R768 T4057 T4056 compound ESG1,sequence
R769 T4058 T4056 compound cDNA,sequence
R770 T4059 T4056 prep as,sequence
R771 T4060 T4061 det a,query
R772 T4061 T4059 pobj query,as
R773 T4062 T4033 punct .,performed
R774 T4064 T4065 nsubj We,identified
R775 T4066 T4067 amod several,pseudogenes
R776 T4067 T4065 dobj pseudogenes,identified
R777 T4068 T4067 amod putative,pseudogenes
R778 T4069 T4067 prep without,pseudogenes
R779 T4070 T4069 pobj introns,without
R780 T4071 T4065 prep on,identified
R781 T4072 T4073 nmod chromosomes,X
R782 T4073 T4071 pobj X,on
R783 T4074 T4073 nummod 1,X
R784 T4075 T4073 punct ", ",X
R785 T4076 T4073 nummod 5,X
R786 T4077 T4073 punct ", ",X
R787 T4078 T4073 nummod 11,X
R788 T4079 T4073 punct ", ",X
R789 T4080 T4073 nummod 12,X
R790 T4081 T4073 punct ", ",X
R791 T4082 T4073 nummod 14,X
R792 T4083 T4073 punct ", ",X
R793 T4084 T4073 nummod 16,X
R794 T4085 T4073 punct ", ",X
R795 T4086 T4073 nummod 17,X
R796 T4087 T4073 punct ", ",X
R797 T4088 T4073 cc and,X
R798 T4089 T4090 punct (,Figure
R799 T4090 T4065 parataxis Figure,identified
R800 T4091 T4090 nummod 1,Figure
R801 T4092 T4090 punct ),Figure
R802 T4093 T4065 punct .,identified
R803 T4095 T4096 prep In,identified
R804 T4097 T4095 pobj addition,In
R805 T4098 T4096 punct ", ",identified
R806 T4099 T4100 nummod two,pseudogenes
R807 T4100 T4096 nsubjpass pseudogenes,identified
R808 T4101 T4100 amod intron-less,pseudogenes
R809 T4102 T4096 auxpass were,identified
R810 T4103 T4096 prep in,identified
R811 T4104 T4105 compound DNA,fragments
R812 T4105 T4103 pobj fragments,in
R813 T4106 T4107 prep for,remained
R814 T4107 T4105 relcl remained,fragments
R815 T4108 T4106 pobj which,for
R816 T4109 T4110 det the,localization
R817 T4110 T4107 nsubj localization,remained
R818 T4111 T4110 amod chromosomal,localization
R819 T4112 T4107 acomp unmapped,remained
R820 T4113 T4096 punct .,identified
R821 T4115 T4116 mark While,have
R822 T4116 T4119 advcl have,produce
R823 T4117 T4118 det these,pseudodgenes
R824 T4118 T4116 nsubj pseudodgenes,have
R825 T4120 T4121 amod significant,homology
R826 T4121 T4116 dobj homology,have
R827 T4122 T4121 prep to,homology
R862 T4161 T4156 attr hallmark,is
R863 T4162 T4161 prep of,hallmark
R864 T4163 T4164 amod pluripotent,genes
R865 T4164 T4162 pobj genes,of
R866 T4165 T4166 npadvmod cell,specific
R867 T4166 T4164 amod specific,genes
R868 T4167 T4166 punct -,specific
R869 T4168 T4169 punct [,18
R870 T4169 T4161 parataxis 18,hallmark
R871 T4170 T4169 punct ],18
R872 T4171 T4156 punct .,is
R873 T4174 T4175 prep On,identified
R874 T4176 T4174 pobj chromosome,On
R875 T4177 T4176 nummod 9,chromosome
R876 T4178 T4175 punct ", ",identified
R877 T4179 T4175 nsubj we,identified
R878 T4180 T4181 det a,fragment
R879 T4181 T4175 dobj fragment,identified
R880 T4182 T4181 compound DNA,fragment
R881 T4183 T4181 amod similar,fragment
R882 T4184 T4183 prep to,similar
R883 T4185 T4186 det the,gene
R884 T4186 T4184 pobj gene,to
R885 T4187 T4186 compound ESG1,gene
R886 T4188 T4189 dep that,included
R887 T4189 T4186 relcl included,gene
R888 T4190 T4191 nummod two,introns
R889 T4191 T4189 dobj introns,included
R890 T4192 T4191 amod putative,introns
R891 T4193 T4175 punct .,identified
R892 T4195 T4196 det These,exons
R893 T4196 T4201 nsubj exons,contained
R894 T4197 T4196 amod putative,exons
R895 T4198 T4196 amod first,exons
R896 T4199 T4198 cc and,first
R897 T4200 T4198 conj second,first
R898 T4202 T4201 punct ", ",contained
R899 T4203 T4201 advmod however,contained
R900 T4204 T4201 punct ", ",contained
R901 T4205 T4206 punct (,4
R902 T4206 T4201 parataxis 4,contained
R903 T4207 T4206 punct ),4
R904 T4208 T4209 amod multiple,mutations
R905 T4209 T4201 dobj mutations,contained
R906 T4210 T4209 prep of,mutations
R907 T4211 T4212 det the,sequence
R908 T4212 T4210 pobj sequence,of
R909 T4213 T4212 compound ESG1,sequence
R910 T4214 T4212 compound cDNA,sequence
R911 T4215 T4201 punct .,contained
R912 T4217 T4218 det The,exon
R913 T4218 T4221 nsubj exon,was
R914 T4219 T4218 amod putative,exon
R915 T4220 T4218 amod third,exon
R916 T4222 T4221 acomp identical,was
R917 T4223 T4222 prep to,identical
R918 T4224 T4223 pobj that,to
R919 T4225 T4224 prep of,that
R920 T4226 T4227 det the,gene
R921 T4227 T4225 pobj gene,of
R922 T4228 T4229 advmod previously,reported
R923 T4229 T4227 amod reported,gene
R924 T4230 T4227 compound ESG1,gene
R925 T4231 T4221 punct .,was
R926 T4233 T4234 advmod Also,identified
R927 T4235 T4234 prep on,identified
R928 T4236 T4235 pobj chromosome,on
R929 T4237 T4236 nummod 9,chromosome
R930 T4238 T4234 punct ", ",identified
R931 T4239 T4234 nsubj we,identified
R932 T4240 T4241 det another,fragment
R933 T4241 T4234 dobj fragment,identified
R934 T4242 T4241 compound DNA,fragment
R935 T4243 T4244 dep that,was
R936 T4244 T4241 relcl was,fragment
R937 T4245 T4244 acomp similar,was
R938 T4246 T4245 punct ", ",similar
R939 T4247 T4245 cc but,similar
R940 T4248 T4247 neg not,but
R941 T4249 T4245 conj identical,similar
R942 T4250 T4249 punct ", ",identical
R943 T4251 T4249 conj to,identical
R944 T4252 T4253 det the,exon
R945 T4253 T4251 pobj exon,to
R946 T4254 T4253 amod third,exon
R947 T4255 T4253 prep of,exon
R948 T4256 T4257 det the,gene
R949 T4257 T4255 pobj gene,of
R950 T4258 T4257 compound ESG1,gene
R951 T4259 T4234 punct .,identified
R952 T4261 T4262 det These,findings
R953 T4262 T4263 nsubj findings,suggest
R954 T4264 T4265 mark that,assembled
R955 T4265 T4263 ccomp assembled,suggest
R956 T4266 T4267 det these,sequences
R957 T4267 T4265 nsubjpass sequences,assembled
R958 T4268 T4269 npadvmod ESG1,like
R959 T4269 T4267 amod like,sequences
R960 T4270 T4269 punct -,like
R961 T4271 T4267 prep on,sequences
R962 T4272 T4271 pobj chromosome,on
R963 T4273 T4272 nummod 9,chromosome
R964 T4274 T4265 aux have,assembled
R965 T4275 T4265 neg not,assembled
R966 T4276 T4265 auxpass been,assembled
R967 T4277 T4265 advmod correctly,assembled
R968 T4278 T4263 punct .,suggest
R969 T4280 T4281 aux To,obtain
R970 T4281 T4282 advcl obtain,screened
R971 T4283 T4284 compound DNA,fragments
R972 T4284 T4281 dobj fragments,obtain
R973 T4285 T4284 acl containing,fragments
R974 T4286 T4287 det the,gene
R975 T4287 T4285 dobj gene,containing
R976 T4288 T4287 compound ESG1,gene
R977 T4289 T4282 punct ", ",screened
R978 T4290 T4282 nsubj we,screened
R979 T4291 T4292 det the,pool
R980 T4292 T4282 dobj pool,screened
R981 T4293 T4294 amod bacterial,chromosome
R982 T4294 T4292 nmod chromosome,pool
R983 T4295 T4294 amod artificial,chromosome
R984 T4296 T4294 punct (,chromosome
R985 T4297 T4294 appos BAC,chromosome
R986 T4298 T4292 punct ),pool
R987 T4299 T4292 compound DNA,pool
R988 T4300 T4282 prep by,screened
R989 T4301 T4300 pobj PCR,by
R990 T4302 T4282 advcl using,screened
R991 T4303 T4304 compound primer,pairs
R992 T4304 T4302 dobj pairs,using
R993 T4305 T4306 dep that,amplify
R994 T4306 T4304 relcl amplify,pairs
R995 T4307 T4306 aux would,amplify
R996 T4308 T4306 advmod only,amplify
R997 T4309 T4310 det the,gene
R998 T4310 T4306 dobj gene,amplify
R999 T4311 T4310 amod real,gene
R1000 T4312 T4306 punct ", ",amplify
R1001 T4313 T4314 neg not,pseudogenes
R1002 T4314 T4306 dep pseudogenes,amplify
R1003 T4315 T4314 det the,pseudogenes
R1004 T4316 T4282 punct .,screened
R1005 T4318 T4319 nsubj We,obtained
R1006 T4320 T4321 nummod two,clones
R1007 T4321 T4319 dobj clones,obtained
R1008 T4322 T4321 amod independent,clones
R1009 T4323 T4322 punct ", ",independent
R1010 T4324 T4322 cc but,independent
R1011 T4325 T4322 conj overlapping,independent
R1012 T4326 T4321 compound BAC,clones
R1013 T4327 T4319 punct .,obtained
R1014 T4329 T4330 compound Southern,blot
R1015 T4330 T4331 nsubj blot,demonstrated
R1016 T4332 T4330 appos analyses,blot
R1017 T4333 T4332 cc and,analyses
R1018 T4334 T4332 conj sequencing,analyses
R1019 T4335 T4336 mark that,contained
R1020 T4336 T4331 ccomp contained,demonstrated
R1021 T4337 T4338 det these,clones
R1022 T4338 T4336 nsubj clones,contained
R1023 T4339 T4340 det a,sequence
R1024 T4340 T4336 dobj sequence,contained
R1025 T4341 T4340 acl exhibiting,sequence
R1026 T4342 T4343 amod complete,identity
R1027 T4343 T4341 dobj identity,exhibiting
R1028 T4344 T4343 prep with,identity
R1029 T4345 T4346 compound ESG1,cDNA
R1030 T4346 T4344 pobj cDNA,with
R1031 T4347 T4348 dep that,interrupted
R1032 T4348 T4340 relcl interrupted,sequence
R1033 T4349 T4348 auxpass was,interrupted
R1034 T4350 T4348 agent by,interrupted
R1035 T4351 T4352 nummod two,introns
R1036 T4352 T4350 pobj introns,by
R1037 T4353 T4352 amod putative,introns
R1038 T4354 T4355 punct (,2A
R1039 T4355 T4348 parataxis 2A,interrupted
R1040 T4356 T4355 compound Figure,2A
R1041 T4357 T4355 punct ),2A
R1042 T4358 T4331 punct .,demonstrated
R1043 T4360 T4361 det The,sequences
R1044 T4361 T4364 nsubj sequences,begin
R1045 T4362 T4361 nummod two,sequences
R1046 T4363 T4361 compound intron,sequences
R1047 T4365 T4364 prep with,begin
R1048 T4366 T4365 pobj GT,with
R1049 T4367 T4364 cc and,begin
R1050 T4368 T4364 conj terminate,begin
R1051 T4369 T4368 prep with,terminate
R1052 T4370 T4369 pobj AG,with
R1053 T4371 T4364 punct ", ",begin
R1054 T4372 T4364 advcl fulfilling,begin
R1055 T4373 T4374 det the,rule
R1056 T4374 T4372 dobj rule,fulfilling
R1057 T4375 T4376 compound GT,AG
R1058 T4376 T4374 compound AG,rule
R1059 T4377 T4376 punct -,AG
R1060 T4378 T4374 prep of,rule
R1061 T4379 T4380 compound exon,intron
R1062 T4380 T4382 compound intron,junctions
R1063 T4381 T4380 punct -,intron
R1064 T4382 T4378 pobj junctions,of
R1065 T4383 T4384 punct [,19
R1066 T4384 T4372 parataxis 19,fulfilling
R1067 T4385 T4384 punct ],19
R1068 T4386 T4364 punct .,begin
R1069 T4388 T4389 det The,region
R1070 T4389 T4393 nsubj region,exhibited
R1071 T4390 T4389 nummod 5,region
R1072 T4391 T4390 punct ',5
R1073 T4392 T4389 compound flanking,region
R1074 T4394 T4389 prep of,region
R1075 T4395 T4396 det this,fragment
R1076 T4396 T4394 pobj fragment,of
R1077 T4397 T4396 compound DNA,fragment
R1078 T4398 T4399 amod strong,activity
R1079 T4399 T4393 dobj activity,exhibited
R1080 T4400 T4401 compound promoter,enhancer
R1081 T4401 T4399 compound enhancer,activity
R1082 T4402 T4401 punct /,enhancer
R1083 T4403 T4393 prep by,exhibited
R1084 T4404 T4405 compound luciferase,assays
R1085 T4405 T4403 pobj assays,by
R1086 T4406 T4405 compound reporter,assays
R1087 T4407 T4393 prep in,exhibited
R1088 T4408 T4409 amod undifferentiated,cells
R1089 T4409 T4407 pobj cells,in
R1090 T4410 T4409 compound ES,cells
R1091 T4411 T4407 punct ", ",in
R1092 T4412 T4407 cc but,in
R1093 T4413 T4412 neg not,but
R1094 T4414 T4407 conj in,in
R1095 T4415 T4416 amod somatic,cells
R1096 T4416 T4414 pobj cells,in
R1097 T4417 T4418 punct (,Figure
R1098 T4418 T4393 parataxis Figure,exhibited
R1099 T4419 T4418 nummod 3,Figure
R1100 T4420 T4418 punct ),Figure
R1101 T4421 T4393 punct .,exhibited
R1102 T4423 T4424 det The,fragment
R1103 T4424 T4426 nsubj fragment,showed
R1104 T4425 T4424 amod same,fragment
R1105 T4427 T4428 advmod much,weaker
R1106 T4428 T4429 amod weaker,activity
R1107 T4429 T4426 dobj activity,showed
R1108 T4430 T4426 prep after,showed
R1109 T4431 T4430 pobj induction,after
R1110 T4432 T4431 prep of,induction
R1111 T4433 T4432 pobj differentiation,of
R1112 T4434 T4431 prep by,induction
R1113 T4435 T4436 amod retinoic,acid
R1114 T4436 T4434 pobj acid,by
R1115 T4437 T4426 punct .,showed
R1116 T4439 T4440 nsubj We,concluded
R1117 T4441 T4442 mark that,is
R1125 T4515 T4513 advmod however,contained
R1126 T4516 T4513 punct ", ",contained
R1127 T4517 T4518 amod critical,substitutions
R1128 T4518 T4513 dobj substitutions,contained
R1129 T4519 T4518 compound nucleotide,substitutions
R1130 T4520 T4513 prep in,contained
R1131 T4521 T4520 pobj all,in
R1132 T4522 T4521 prep of,all
R1133 T4523 T4524 det the,exons
R1134 T4524 T4522 pobj exons,of
R1135 T4525 T4513 cc and,contained
R1136 T4526 T4527 nummod one,insertion
R1137 T4527 T4513 conj insertion,contained
R1138 T4528 T4527 compound nucleotide,insertion
R1139 T4529 T4527 prep in,insertion
R1140 T4530 T4529 pobj exon,in
R1142 T4531 T4530 nummod 2,exon
R1143 T4532 T4533 punct (,2B
R1146 T4533 T4527 parataxis 2B,insertion
R1147 T4534 T4533 compound Figure,2B
R1148 T4454 T4451 ccomp contained,found
R1149 T4535 T4533 punct ),2B
R1151 T4456 T4454 nsubj clones,contained
R1152 T4536 T4513 punct .,contained
R1155 T4459 T4460 det another,sequence
R1156 T4460 T4454 dobj sequence,contained
R1157 T4461 T4462 npadvmod ESG1,like
R1158 T4462 T4460 amod like,sequence
R1159 T4538 T4539 mark Although,conserved
R1160 T4463 T4462 punct -,like
R1161 T4464 T4465 punct (,2A
R1162 T4465 T4454 parataxis 2A,contained
R1163 T4539 T4550 advcl conserved,were
R1164 T4466 T4465 compound Figure,2A
R1165 T4467 T4465 punct ),2A
R1166 T4468 T4451 punct .,found
R1167 T4540 T4541 nummod 675,pairs
R1168 T4470 T4471 det The,sequences
R1169 T4471 T4473 nsubjpass sequences,oriented
R1170 T4541 T4539 nsubjpass pairs,conserved
R1171 T4472 T4471 nummod two,sequences
R1172 T4474 T4471 punct ", ",sequences
R1173 T4542 T4541 compound base,pairs
R1174 T4475 T4471 acl separated,sequences
R1175 T4476 T4475 agent by,separated
R1176 T4543 T4541 prep of,pairs
R1177 T4477 T4478 det a,sequence
R1178 T4478 T4476 pobj sequence,by
R1179 T4479 T4480 nummod 68,kbp
R1180 T4544 T4545 det the,regions
R1181 T4480 T4478 nmod kbp,sequence
R1182 T4481 T4478 amod intergenic,sequence
R1183 T4545 T4543 pobj regions,of
R1184 T4482 T4473 punct ", ",oriented
R1185 T4546 T4545 nummod 3,regions
R1186 T4483 T4473 auxpass were,oriented
R1187 T4484 T4473 prep in,oriented
R1188 T4485 T4486 amod opposite,directions
R1189 T4486 T4484 pobj directions,in
R1190 T4487 T4473 punct .,oriented
R1191 T4547 T4546 punct ',3
R1192 T4489 T4490 det The,sequence
R1193 T4490 T4494 nsubj sequence,exhibited
R1194 T4548 T4545 compound flanking,regions
R1195 T4491 T4492 npadvmod ESG1,like
R1196 T4492 T4490 amod like,sequence
R1197 T4493 T4492 punct -,like
R1198 T4549 T4539 auxpass were,conserved
R1199 T4495 T4496 amod greater,95
R1200 T4496 T4498 nummod 95,%
R1201 T4551 T4539 prep between,conserved
R1202 T4497 T4496 quantmod than,95
R1203 T4498 T4499 compound %,identity
R1204 T4499 T4494 dobj identity,exhibited
R1205 T4500 T4499 prep to,identity
R1206 T4552 T4553 det the,gene
R1207 T4501 T4502 det the,exons
R1208 T4502 T4500 pobj exons,to
R1209 T4503 T4502 cc and,exons
R1210 T4504 T4502 conj introns,exons
R1211 T4505 T4502 prep of,exons
R1212 T4553 T4551 pobj gene,between
R1213 T4506 T4507 det the,gene
R1214 T4507 T4505 pobj gene,of
R1215 T4508 T4507 compound ESG1,gene
R1216 T4554 T4553 compound ESG1,gene
R1217 T4509 T4494 punct .,exhibited
R1218 T4511 T4512 det This,sequence
R1219 T4555 T4553 cc and,gene
R1220 T4512 T4513 nsubj sequence,contained
R1221 T4556 T4557 det the,pseudogene
R1222 T4514 T4513 punct ", ",contained
R1223 T4557 T4553 conj pseudogene,gene
R1224 T4558 T4550 punct ", ",were
R1225 T4559 T4560 advmod only,pairs
R1226 T4621 T4622 det a,pseudogene
R1227 T4560 T4550 nsubj pairs,were
R1228 T4622 T4619 dobj pseudogene,represents
R1229 T4623 T4622 compound duplication,pseudogene
R1230 T4624 T4619 punct .,represents
R1231 T4561 T4560 nummod five,pairs
R1232 T4626 T4627 nsubj Bierbaum,reported
R1233 T4562 T4560 compound base,pairs
R1234 T4628 T4627 advmod previously,reported
R1235 T4629 T4630 det the,existence
R1236 T4563 T4560 prep of,pairs
R1237 T4630 T4627 dobj existence,reported
R1238 T4631 T4630 prep of,existence
R1239 T4632 T4633 nummod two,pseudogenes
R1240 T4564 T4565 det the,region
R1241 T4633 T4631 pobj pseudogenes,of
R1242 T4565 T4563 pobj region,of
R1243 T4634 T4633 prep with,pseudogenes
R1244 T4566 T4565 nummod 5,region
R1245 T4635 T4636 amod similar,organization
R1246 T4567 T4566 punct ',5
R1247 T4636 T4634 pobj organization,with
R1248 T4637 T4638 compound exon,intron
R1249 T4568 T4565 compound flanking,region
R1250 T4638 T4636 compound intron,organization
R1251 T4639 T4638 punct -,intron
R1252 T4640 T4627 prep as,reported
R1253 T4569 T4550 acomp identical,were
R1254 T4641 T4642 det the,gene
R1255 T4642 T4640 pobj gene,as
R1256 T4643 T4642 compound ESG1,gene
R1257 T4570 T4550 punct .,were
R1258 T4644 T4645 punct [,17
R1259 T4645 T4627 parataxis 17,reported
R1260 T4646 T4645 punct ],17
R1261 T4647 T4627 punct .,reported
R1262 T4572 T4573 det This,region
R1263 T4649 T4650 nsubj We,determine
R1264 T4651 T4650 aux could,determine
R1265 T4573 T4577 nsubj region,possess
R1266 T4652 T4650 neg not,determine
R1267 T4653 T4654 dep which,corresponds
R1268 T4654 T4650 ccomp corresponds,determine
R1269 T4574 T4573 nummod 5,region
R1270 T4655 T4653 prep of,which
R1271 T4656 T4657 det these,pseudogenes
R1272 T4575 T4574 punct ',5
R1273 T4657 T4655 pobj pseudogenes,of
R1274 T4658 T4657 nummod two,pseudogenes
R1275 T4576 T4573 compound flanking,region
R1276 T4659 T4654 prep to,corresponds
R1277 T4660 T4661 det the,one
R1278 T4661 T4659 pobj one,to
R1279 T4578 T4579 punct (,kbp
R1280 T4662 T4663 nsubj we,identified
R1281 T4663 T4661 advcl identified,one
R1282 T4664 T4661 cc or,one
R1283 T4665 T4666 det the,location
R1284 T4579 T4573 parataxis kbp,region
R1285 T4666 T4661 conj location,one
R1286 T4667 T4666 prep of,location
R1287 T4668 T4669 det the,pseudogene
R1288 T4580 T4581 punct ~,6
R1289 T4669 T4667 pobj pseudogene,of
R1290 T4670 T4669 amod remaining,pseudogene
R1291 T4671 T4650 punct .,determine
R1292 T4581 T4579 nummod 6,kbp
R1293 T4582 T4579 punct ),kbp
R1294 T4583 T4577 aux did,possess
R1295 T4584 T4577 neg not,possess
R1296 T4585 T4586 det any,activity
R1297 T4586 T4577 dobj activity,possess
R1298 T4587 T4588 compound promoter,enhancer
R1299 T4588 T4586 compound enhancer,activity
R1300 T4589 T4588 punct /,enhancer
R1301 T4590 T4577 prep in,possess
R1302 T4591 T4592 compound luciferase,reporter
R1303 T4592 T4593 compound reporter,assays
R1304 T4593 T4590 pobj assays,in
R1305 T4594 T4595 punct (,Figure
R1306 T4595 T4577 parataxis Figure,possess
R1307 T4596 T4595 nummod 3,Figure
R1308 T4597 T4595 punct ),Figure
R1309 T4598 T4577 punct .,possess
R1310 T4600 T4601 nsubj It,is
R1311 T4602 T4601 advmod thus,is
R1312 T4603 T4601 acomp unlikely,is
R1313 T4604 T4605 mark that,transcribed
R1314 T4605 T4601 ccomp transcribed,is
R1315 T4606 T4607 det this,sequence
R1316 T4607 T4605 nsubjpass sequence,transcribed
R1317 T4608 T4605 auxpass is,transcribed
R1318 T4609 T4605 cc or,transcribed
R1319 T4610 T4605 conj translated,transcribed
R1320 T4611 T4610 prep into,translated
R1321 T4612 T4613 det a,protein
R1322 T4613 T4611 pobj protein,into
R1323 T4614 T4613 amod functional,protein
R1324 T4615 T4601 punct .,is
R1325 T4617 T4618 det This,sequence
R1326 T4618 T4619 nsubj sequence,represents
R1327 T4620 T4619 advmod likely,represents
R1329 T6437 T6438 amod Targeted,disruption
R1330 T6439 T6438 prep of,disruption
R1331 T6440 T6441 det the,gene
R1332 T6441 T6439 pobj gene,of
R1333 T6442 T6441 compound mouse,gene
R1334 T6443 T6441 compound ESG1,gene
R1335 T6445 T6446 aux To,study
R1336 T6446 T6447 advcl study,deleted
R1337 T6448 T6449 det the,function
R1338 T6449 T6446 dobj function,study
R1339 T6450 T6449 prep of,function
R1340 T6451 T6450 pobj ESG1,of
R1341 T6452 T6447 punct ", ",deleted
R1342 T6453 T6447 nsubj we,deleted
R1343 T6454 T6455 det the,gene
R1344 T6455 T6447 dobj gene,deleted
R1345 T6456 T6447 prep by,deleted
R1346 T6457 T6458 amod homologous,recombination
R1347 T6458 T6456 pobj recombination,by
R1348 T6459 T6458 prep in,recombination
R1349 T6460 T6461 compound mouse,cells
R1350 T6461 T6459 pobj cells,in
R1351 T6462 T6461 compound ES,cells
R1352 T6463 T6447 punct .,deleted
R1353 T6465 T6466 nsubj We,replaced
R1354 T6467 T6468 det the,exons
R1355 T6468 T6466 dobj exons,replaced
R1356 T6469 T6468 nummod three,exons
R1357 T6470 T6466 prep with,replaced
R1358 T6471 T6472 preconj either,fusion
R1359 T6472 T6470 pobj fusion,with
R1360 T6473 T6472 det a,fusion
R1361 T6474 T6472 prep of,fusion
R1362 T6475 T6476 det the,genes
R1363 T6476 T6474 pobj genes,of
R1364 T6477 T6478 nmod neomycin,resistance
R1365 T6478 T6476 nmod resistance,genes
R1366 T6479 T6478 punct -,resistance
R1367 T6480 T6478 cc and,resistance
R1368 T6481 T6482 compound β,galactosidase
R1369 T6482 T6478 conj galactosidase,resistance
R1370 T6483 T6482 punct -,galactosidase
R1371 T6484 T6485 punct (,geo
R1372 T6485 T6476 parataxis geo,genes
R1373 T6486 T6485 compound β,geo
R1374 T6487 T6485 punct -,geo
R1386 T6499 T6497 dobj vectors,using
R1387 T6500 T6499 compound targeting,vectors
R1388 T6501 T6502 punct (,4A
R1389 T6502 T6499 parataxis 4A,vectors
R1390 T6503 T6502 compound Figure,4A
R1391 T6504 T6502 punct ),4A
R1392 T6505 T6499 acl introduced,vectors
R1393 T6506 T6505 prep into,introduced
R1394 T6507 T6508 compound RF8,cells
R1395 T6508 T6506 pobj cells,into
R1396 T6509 T6508 compound ES,cells
R1397 T6510 T6505 prep by,introduced
R1398 T6511 T6510 pobj electroporation,by
R1399 T6512 T6466 punct .,replaced
R1400 T6514 T6515 nsubj We,obtained
R1401 T6516 T6517 nummod eight,clones
R1402 T6517 T6515 dobj clones,obtained
R1403 T6518 T6517 compound ES,clones
R1404 T6519 T6517 compound cell,clones
R1405 T6520 T6515 prep with,obtained
R1406 T6521 T6522 amod correct,recombination
R1407 T6522 T6520 pobj recombination,with
R1408 T6523 T6522 amod homologous,recombination
R1409 T6524 T6522 prep of,recombination
R1410 T6525 T6526 det the,vector
R1411 T6526 T6524 pobj vector,of
R1412 T6527 T6528 compound β,geo
R1413 T6528 T6526 compound geo,vector
R1414 T6529 T6528 punct -,geo
R1415 T6530 T6526 compound targeting,vector
R1416 T6531 T6522 punct ", ",recombination
R1417 T6532 T6533 dep which,confirmed
R1418 T6533 T6522 relcl confirmed,recombination
R1419 T6534 T6533 auxpass was,confirmed
R1420 T6535 T6533 agent by,confirmed
R1421 T6536 T6537 compound Southern,blot
R1422 T6537 T6538 compound blot,analysis
R1423 T6538 T6535 pobj analysis,by
R1424 T6539 T6540 punct (,4B
R1425 T6540 T6533 parataxis 4B,confirmed
R1426 T6541 T6540 compound Figure,4B
R1427 T6542 T6540 punct ),4B
R1428 T6543 T6515 punct .,obtained
R1429 T6545 T6546 nsubj We,obtained
R1430 T6547 T6548 advmod only,clone
R1431 T6548 T6546 dobj clone,obtained
R1432 T6549 T6548 nummod one,clone
R1433 T6550 T6548 prep with,clone
R1434 T6551 T6552 amod correct,recombination
R1435 T6552 T6550 pobj recombination,with
R1436 T6553 T6552 amod homologous,recombination
R1437 T6554 T6552 prep of,recombination
R1438 T6555 T6556 det the,vector
R1439 T6556 T6554 pobj vector,of
R1440 T6557 T6556 compound HygR,vector
R1441 T6558 T6556 compound targeting,vector
R1442 T6559 T6546 punct .,obtained
R1443 T6562 T6563 amod T,o
R1444 T6564 T6563 prep o,o
R1469 T6613 T6604 acomp homozygous,were
R1470 T6614 T6613 prep for,homozygous
R1471 T6615 T6616 compound ESG1,deletion
R1472 T6616 T6614 pobj deletion,for
R1473 T6617 T6604 punct .,were
R1474 T6619 T6620 nmod Northern,blot
R1475 T6620 T6621 nmod blot,analyses
R1476 T6621 T6625 nsubj analyses,confirmed
R1477 T6622 T6620 cc and,blot
R1478 T6623 T6624 compound western,blot
R1479 T6624 T6620 conj blot,blot
R1480 T6626 T6627 det the,absence
R1481 T6627 T6625 dobj absence,confirmed
R1482 T6628 T6627 prep of,absence
R1483 T6629 T6628 pobj ESG1,of
R1484 T6630 T6627 prep in,absence
R1485 T6631 T6632 det these,cells
R1486 T6632 T6630 pobj cells,in
R1487 T6633 T6634 punct (,4C
R1488 T6634 T6625 parataxis 4C,confirmed
R1489 T6635 T6634 compound Figure,4C
R1490 T6636 T6634 punct ),4C
R1491 T6637 T6625 punct .,confirmed
R1492 T6639 T6640 prep In,replaced
R1493 T6641 T6642 nummod 29,clones
R1494 T6642 T6639 pobj clones,In
R1495 T6643 T6640 punct ", ",replaced
R1496 T6644 T6645 det the,vector
R1497 T6645 T6640 nsubj vector,replaced
R1498 T6646 T6647 compound β,geo
R1499 T6647 T6645 compound geo,vector
R1500 T6648 T6647 punct -,geo
R1501 T6649 T6640 aux had,replaced
R1502 T6650 T6651 det the,vector
R1503 T6651 T6640 dobj vector,replaced
R1504 T6652 T6651 compound HygR,vector
R1505 T6653 T6640 punct ", ",replaced
R1506 T6654 T6655 amod such,remained
R1507 T6655 T6640 advcl remained,replaced
R1508 T6656 T6655 mark that,remained
R1509 T6657 T6658 det the,cells
R1510 T6658 T6655 nsubj cells,remained
R1511 T6659 T6655 acomp heterozygous,remained
R1512 T6660 T6640 punct .,replaced
R1513 T6662 T6663 prep In,integrated
R1514 T6664 T6665 det the,clones
R1515 T6665 T6662 pobj clones,In
R1516 T6666 T6665 amod remaining,clones
R1517 T6667 T6665 nummod 27,clones
R1518 T6668 T6663 punct ", ",integrated
R1519 T6669 T6670 det the,vector
R1520 T6670 T6663 nsubjpass vector,integrated
R1521 T6671 T6672 compound β,geo
R1522 T6672 T6670 compound geo,vector
R1523 T6673 T6672 punct -,geo
R1524 T6674 T6663 auxpass was,integrated
R1525 T6675 T6663 prep at,integrated
R1526 T6676 T6677 amod non-homologous,sites
R1527 T6677 T6675 pobj sites,at
R1528 T6678 T6663 punct .,integrated
R1529 T6680 T6681 nmod ESG1,cells
R1530 T6681 T6686 nsubj cells,exhibited
R1531 T6682 T6680 punct -,ESG1
R1532 T6683 T6680 punct /,ESG1
R1533 T6684 T6680 punct -,ESG1
R1534 T6685 T6681 compound ES,cells
R1535 T6687 T6688 amod normal,morphology
R1536 T6688 T6686 dobj morphology,exhibited
R1537 T6689 T6690 punct (,5A
R1538 T6690 T6686 parataxis 5A,exhibited
R1539 T6691 T6690 compound Figure,5A
R1540 T6692 T6690 punct ),5A
R1541 T6693 T6686 punct .,exhibited
R1542 T6695 T6696 det These,cells
R1543 T6696 T6697 nsubj cells,proliferated
R1544 T6698 T6697 advmod also,proliferated
R1545 T6699 T6697 prep at,proliferated
R1546 T6700 T6701 det a,speed
R1547 T6701 T6699 pobj speed,at
R1548 T6702 T6701 amod comparable,speed
R1549 T6703 T6702 prep to,comparable
R1550 T6704 T6703 pobj that,to
R1551 T6705 T6704 prep of,that
R1552 T6706 T6707 det the,cells
R1553 T6707 T6705 pobj cells,of
R1554 T6708 T6707 nmod control,cells
R1555 T6709 T6710 punct (,heterozygous
R1556 T6710 T6708 parataxis heterozygous,control
R1557 T6711 T6710 cc and,heterozygous
R1558 T6712 T6713 amod wild,type
R1559 T6713 T6710 conj type,heterozygous
R1560 T6714 T6713 punct -,type
R1561 T6715 T6710 punct ),heterozygous
R1562 T6716 T6717 punct (,5B
R1563 T6717 T6697 parataxis 5B,proliferated
R1564 T6718 T6717 compound Figure,5B
R1565 T6719 T6717 punct ),5B
R1566 T6720 T6697 punct .,proliferated
R1567 T6722 T6723 nmod ESG1,cells
R1568 T6723 T6727 nsubj cells,differentiated
R1569 T6724 T6722 punct -,ESG1
R1570 T6725 T6722 punct /,ESG1
R1571 T6726 T6722 punct -,ESG1
R1572 T6728 T6727 advmod normally,differentiated
R1573 T6729 T6727 prep after,differentiated
R1574 T6730 T6731 det the,removal
R1575 T6731 T6729 pobj removal,after
R1576 T6732 T6731 prep of,removal
R1577 T6733 T6734 nmod leukemia,factor
R1578 T6734 T6732 pobj factor,of
R1579 T6735 T6734 amod inhibitory,factor
R1580 T6736 T6737 punct (,5B
R1581 T6737 T6734 parataxis 5B,factor
R1582 T6738 T6737 compound Figure,5B
R1583 T6739 T6737 punct ),5B
R1584 T6740 T6731 cc or,removal
R1614 T6772 T6773 punct (,5C
R1615 T6773 T6752 parataxis 5C,produced
R1616 T6774 T6773 compound Figure,5C
R1617 T6775 T6773 punct ),5C
R1618 T6776 T6752 punct .,produced
R1619 T6778 T6779 det These,results
R1620 T6779 T6780 nsubj results,indicate
R1621 T6781 T6782 mark that,is
R1622 T6782 T6780 ccomp is,indicate
R1623 T6783 T6782 nsubj ESG1,is
R1624 T6784 T6782 acomp dispensable,is
R1625 T6785 T6784 prep for,dispensable
R1626 T6786 T6787 det the,properties
R1627 T6787 T6785 pobj properties,for
R1628 T6788 T6789 compound self,renewal
R1629 T6789 T6787 compound renewal,properties
R1630 T6790 T6789 punct -,renewal
R1631 T6791 T6787 cc and,properties
R1632 T6792 T6787 conj pluripotency,properties
R1633 T6793 T6792 prep of,pluripotency
R1634 T6794 T6795 compound ES,cells
R1635 T6795 T6793 pobj cells,of
R1636 T6796 T6780 punct .,indicate
R1653 T6822 T6818 dobj microarrays,using
R1654 T6823 T6822 compound DNA,microarrays
R1655 T6824 T6822 acl representing,microarrays
R1656 T6825 T6826 punct ~,"20,000"
R1657 T6826 T6827 nummod "20,000",genes
R1658 T6827 T6824 dobj genes,representing
R1659 T6828 T6806 punct .,mined
R1660 T6830 T6831 prep In,identified
R1661 T6832 T6830 pobj comparison,In
R1662 T6833 T6832 prep to,comparison
R1663 T6834 T6835 compound control,cells
R1664 T6835 T6833 pobj cells,to
R1665 T6836 T6835 compound ES,cells
R1666 T6837 T6831 punct ", ",identified
R1667 T6838 T6831 nsubjpass ESG1,identified
R1668 T6839 T6831 auxpass was,identified
R1669 T6840 T6831 prep as,identified
R1670 T6841 T6842 det the,gene
R1671 T6842 T6840 pobj gene,as
R1672 T6843 T6842 acl reduced,gene
R1673 T6844 T6843 prep to,reduced
R1674 T6845 T6846 det the,extent
R1675 T6846 T6844 pobj extent,to
R1676 T6847 T6846 amod greatest,extent
R1677 T6848 T6843 prep in,reduced
R1678 T6849 T6850 nmod ESG1,cells
R1679 T6850 T6848 pobj cells,in
R1680 T6851 T6849 punct -,ESG1
R1681 T6852 T6849 punct /,ESG1
R1682 T6853 T6849 punct -,ESG1
R1683 T6854 T6850 compound ES,cells
R1684 T6855 T6856 punct (,6A
R1685 T6856 T6842 parataxis 6A,gene
R1686 T6857 T6856 compound Figure,6A
R1687 T6858 T6856 punct ),6A
R1688 T6859 T6831 punct .,identified
R1689 T6861 T6862 det The,expression
R1690 T6862 T6863 nsubj expression,was
R1691 T6864 T6862 prep of,expression
R1692 T6865 T6866 compound ES,cell
R1693 T6866 T6867 compound cell,genes
R1694 T6867 T6864 pobj genes,of
R1695 T6868 T6867 compound marker,genes
R1696 T6869 T6867 punct ", ",genes
R1697 T6870 T6871 amod such,as
R1698 T6871 T6867 prep as,genes
R1699 T6872 T6871 pobj Nanog,as
R1700 T6873 T6872 cc and,Nanog
R1701 T6874 T6872 conj Oct3,Nanog
R1702 T6875 T6874 punct /,Oct3
R1703 T6876 T6874 nummod 4,Oct3
R1704 T6877 T6863 punct ", ",was
R1705 T6878 T6863 acomp normal,was
R1706 T6879 T6863 prep in,was
R1707 T6880 T6881 nmod ESG1,cells
R1708 T6881 T6879 pobj cells,in
R1709 T6882 T6880 punct -,ESG1
R1710 T6883 T6880 punct /,ESG1
R1711 T6884 T6880 punct -,ESG1
R1712 T6885 T6881 compound ES,cells
R1713 T6886 T6863 punct .,was
R1714 T6888 T6889 nsubj We,confirmed
R1715 T6890 T6891 amod normal,expression
R1716 T6891 T6889 dobj expression,confirmed
R1717 T6892 T6891 nmod Oct3,expression
R1718 T6893 T6892 punct /,Oct3
R1719 T6894 T6892 nummod 4,Oct3
R1720 T6895 T6891 prep at,expression
R1721 T6896 T6897 compound protein,levels
R1722 T6897 T6895 pobj levels,at
R1723 T6898 T6889 prep by,confirmed
R1724 T6899 T6900 compound Western,blot
R1725 T6900 T6898 pobj blot,by
R1726 T6901 T6902 punct (,6B
R1727 T6902 T6889 parataxis 6B,confirmed
R1728 T6903 T6902 compound Figure,6B
R1729 T6904 T6902 punct ),6B
R1730 T6905 T6889 punct .,confirmed
R1731 T6907 T6908 det The,profiles
R1732 T6908 T6912 nsubj profiles,were
R1733 T6909 T6908 amod overall,profiles
R1734 T6910 T6911 compound gene,expression
R1735 T6911 T6908 compound expression,profiles
R1736 T6913 T6912 acomp similar,were
R1737 T6914 T6912 prep between,were
R1738 T6915 T6916 compound control,cells
R1739 T6916 T6914 pobj cells,between
R1740 T6917 T6916 compound ES,cells
R1741 T6918 T6916 cc and,cells
R1742 T6919 T6920 nmod ESG1,cells
R1743 T6920 T6916 conj cells,cells
R1744 T6921 T6919 punct -,ESG1
R1745 T6922 T6919 punct /,ESG1
R1746 T6923 T6919 punct -,ESG1
R1747 T6924 T6920 compound ES,cells
R1748 T6925 T6912 punct .,were
R1749 T6927 T6928 amod Several,genes
R1750 T6928 T6929 nsubj genes,exhibited
R1751 T6930 T6931 det a,reduction
R1752 T6931 T6929 dobj reduction,exhibited
R1753 T6932 T6933 amod greater,two-fold
R1754 T6933 T6931 nummod two-fold,reduction
R1755 T6934 T6933 quantmod than,two-fold
R1756 T6935 T6929 prep in,exhibited
R1757 T6936 T6937 nmod ESG1,cells
R1758 T6937 T6935 pobj cells,in
R1759 T6938 T6936 punct -,ESG1
R1760 T6939 T6936 punct /,ESG1
R1761 T6940 T6936 punct -,ESG1
R1762 T6941 T6929 punct ", ",exhibited
R1763 T6942 T6929 prep including,exhibited
R1764 T6943 T6942 pobj Krt1,including
R1765 T6944 T6943 punct -,Krt1
R1766 T6945 T6943 nummod 8,Krt1
R1767 T6946 T6943 punct ", ",Krt1
R1768 T6947 T6943 conj Pem,Krt1
R1769 T6948 T6947 punct ", ",Pem
R1770 T6949 T6947 conj Ctgf,Pem
R1771 T6950 T6949 punct ", ",Ctgf
R1772 T6951 T6949 conj Ptgs2,Ctgf
R1773 T6952 T6951 punct ", ",Ptgs2
R1774 T6953 T6951 conj Igf2,Ptgs2
R1775 T6954 T6953 cc and,Igf2
R1776 T6955 T6953 conj Inhba,Igf2
R1777 T6956 T6929 punct .,exhibited
R1778 T6958 T6959 det These,genes
R1779 T6959 T6960 nsubjpass genes,regulated
R1780 T6961 T6960 aux might,regulated
R1781 T6962 T6960 auxpass be,regulated
R1782 T6963 T6960 advmod directly,regulated
R1857 T7059 T7060 nummod 36,type
R1858 T7060 T7056 appos type,ratios
R1859 T7061 T7060 amod wild,type
R1860 T7062 T7060 punct -,type
R1861 T7063 T7060 punct ", ",type
R1862 T7064 T7065 nummod 69,ESG1
R1863 T7065 T7060 conj ESG1,type
R1864 T7066 T7065 punct +,ESG1
R1865 T7067 T7065 punct /,ESG1
R1866 T7068 T7065 punct -,ESG1
R1867 T7069 T7065 punct ", ",ESG1
R1868 T7070 T7065 cc and,ESG1
R1869 T7071 T7072 nummod 45,ESG1
R1870 T7072 T7065 conj ESG1,ESG1
R1871 T7073 T7072 punct -,ESG1
R1872 T7074 T7072 punct /,ESG1
R1873 T7075 T7072 punct -,ESG1
R1874 T7076 T7056 punct ),ratios
R1875 T7077 T7048 prep from,obtained
R1876 T7078 T7077 pobj intercrosses,from
R1877 T7079 T7078 prep of,intercrosses
R1878 T7080 T7081 nmod ESG1,mice
R1879 T7081 T7079 pobj mice,of
R1880 T7082 T7080 punct +,ESG1
R1881 T7083 T7080 punct /,ESG1
R1882 T7084 T7080 punct -,ESG1
R1883 T7085 T7048 punct .,obtained
R1884 T7087 T7088 det These,animals
R1885 T7088 T7089 nsubj animals,exhibited
R1886 T7090 T7091 amod normal,development
R1887 T7091 T7089 dobj development,exhibited
R1888 T7092 T7091 punct ", ",development
R1889 T7093 T7094 amod gross,appearance
R1890 T7094 T7091 conj appearance,development
R1891 T7095 T7094 punct ", ",appearance
R1892 T7096 T7094 cc and,appearance
R1893 T7097 T7094 conj fertility,appearance
R1894 T7098 T7099 punct (,shown
R1895 T7099 T7089 parataxis shown,exhibited
R1896 T7100 T7099 neg not,shown
R1897 T7121 T7122 det These,data
R1898 T7101 T7099 punct ),shown
R1899 T7122 T7123 nsubj data,demonstrated
R1900 T7102 T7089 punct .,exhibited
R1901 T7124 T7125 mark that,is
R1902 T7125 T7123 ccomp is,demonstrated
R1903 T7126 T7125 nsubj ESG1,is
R1904 T7127 T7125 acomp dispensable,is
R1905 T7128 T7127 prep for,dispensable
R1906 T7129 T7130 preconj both,development
R1907 T7130 T7128 pobj development,for
R1908 T7131 T7130 compound mouse,development
R1909 T7104 T7105 amod Histological,examination
R1910 T7132 T7130 cc and,development
R1911 T7133 T7134 compound germ,cell
R1912 T7134 T7135 compound cell,formation
R1913 T7135 T7130 conj formation,development
R1914 T7136 T7123 punct .,demonstrated
R1915 T7138 T7139 nsubj We,generated
R1916 T7105 T7106 nsubj examination,identify
R1917 T7140 T7139 advmod also,generated
R1918 T7141 T7142 compound ES,cells
R1919 T7142 T7139 dobj cells,generated
R1920 T7107 T7105 prep of,examination
R1921 T7143 T7139 prep from,generated
R1922 T7144 T7143 pobj blastocysts,from
R1923 T7145 T7144 acl obtained,blastocysts
R1924 T7146 T7145 prep by,obtained
R1925 T7108 T7107 pobj testis,of
R1926 T7147 T7146 pobj intercrosses,by
R1927 T7148 T7147 prep of,intercrosses
R1928 T7109 T7108 cc and,testis
R1929 T7149 T7150 nmod ESG1,males
R1930 T7110 T7108 conj ovary,testis
R1931 T7150 T7148 pobj males,of
R1932 T7151 T7149 punct +,ESG1
R1933 T7152 T7149 punct /,ESG1
R1934 T7153 T7149 punct -,ESG1
R1935 T7111 T7106 aux could,identify
R1936 T7154 T7150 cc and,males
R1937 T7155 T7156 nmod ESG1,females
R1938 T7112 T7106 neg not,identify
R1939 T7156 T7150 conj females,males
R1940 T7157 T7155 punct -,ESG1
R1941 T7158 T7155 punct /,ESG1
R1942 T7159 T7155 punct -,ESG1
R1943 T7113 T7114 det any,abnormalities
R1944 T7160 T7139 punct .,generated
R1945 T7162 T7163 prep Of,were
R1946 T7114 T7106 dobj abnormalities,identify
R1947 T7164 T7165 det the,lines
R1948 T7165 T7162 pobj lines,Of
R1949 T7166 T7165 nummod eight,lines
R1950 T7115 T7116 punct (,shown
R1951 T7167 T7165 compound ES,lines
R1952 T7168 T7165 compound cell,lines
R1953 T7169 T7165 acl established,lines
R1954 T7116 T7106 parataxis shown,identify
R1955 T7170 T7163 punct ", ",were
R1956 T7171 T7172 nummod two,clones
R1957 T7172 T7163 nsubj clones,were
R1958 T7173 T7163 attr ESG1,were
R1959 T7174 T7173 punct -,ESG1
R1960 T7175 T7173 punct /,ESG1
R1961 T7176 T7173 punct -,ESG1
R1962 T7177 T7163 punct .,were
R1963 T7117 T7116 neg not,shown
R1964 T7179 T7180 det These,cells
R1965 T7180 T7185 nsubj cells,demonstrated
R1966 T7181 T7182 npadvmod ESG1,null
R1967 T7118 T7116 punct ),shown
R1968 T7182 T7180 amod null,cells
R1969 T7183 T7182 punct -,null
R1970 T7184 T7180 compound ES,cells
R1971 T7119 T7106 punct .,identify
R1972 T7186 T7187 amod normal,morphology
R1973 T7187 T7185 dobj morphology,demonstrated
R1974 T7188 T7187 punct ", ",morphology
R1975 T7189 T7187 conj proliferation,morphology
R1976 T7190 T7189 punct ", ",proliferation
R1977 T7191 T7189 cc and,proliferation
R1978 T7192 T7189 conj differentiation,proliferation
R1979 T7193 T7194 punct (,shown
R1980 T7194 T7192 parataxis shown,differentiation
R1981 T7195 T7194 neg not,shown
R1982 T7196 T7194 punct ),shown
R1983 T7197 T7185 punct ", ",demonstrated
R1984 T7198 T7185 advcl confirming,demonstrated
R1985 T7199 T7200 mark that,is
R1986 T7200 T7198 ccomp is,confirming
R1987 T7201 T7200 nsubj ESG1,is
R1988 T7202 T7200 acomp dispensable,is
R1989 T7203 T7200 prep in,is
R1990 T7204 T7205 compound ES,cells
R1991 T7205 T7203 pobj cells,in
R1992 T7206 T7185 punct .,demonstrated
R1993 T7369 T7370 aux To,analyze
R1994 T7370 T7371 advcl analyze,identified
R1995 T7372 T7373 det the,roles
R1996 T7373 T7370 dobj roles,analyze
R1997 T7374 T7373 amod physiological,roles
R1998 T7375 T7373 prep of,roles
R1999 T7376 T7375 pobj ESG1,of
R2000 T7377 T7371 punct ", ",identified
R2001 T7378 T7371 nsubj we,identified
R2002 T7379 T7380 det the,gene
R2003 T7380 T7371 dobj gene,identified
R2004 T7381 T7380 compound mouse,gene
R2005 T7382 T7371 prep on,identified
R2006 T7383 T7382 pobj chromosome,on
R2007 T7384 T7383 nummod 9,chromosome
R2008 T7385 T7371 cc and,identified
R2009 T7386 T7371 conj deleted,identified
R2010 T7387 T7386 dobj it,deleted
R2011 T7388 T7386 prep by,deleted
R2012 T7389 T7390 amod homologous,recombination
R2013 T7390 T7388 pobj recombination,by
R2014 T7391 T7386 prep in,deleted
R2015 T7392 T7393 compound ES,cells
R2016 T7393 T7391 pobj cells,in
R2017 T7394 T7371 punct .,identified
R2018 T7396 T7397 prep Despite,demonstrated
R2019 T7398 T7399 amod specific,expression
R2020 T7399 T7396 pobj expression,Despite
R2021 T7400 T7399 prep in,expression
R2022 T7401 T7402 amod early,embryos
R2023 T7402 T7400 pobj embryos,in
R2024 T7403 T7402 punct ", ",embryos
R2025 T7404 T7405 compound germ,cells
R2026 T7405 T7402 conj cells,embryos
R2027 T7406 T7405 punct ", ",cells
R2028 T7407 T7405 cc and,cells
R2029 T7408 T7409 amod pluripotent,cells
R2030 T7409 T7405 conj cells,cells
R2031 T7410 T7397 punct ", ",demonstrated
R2032 T7411 T7412 poss our,data
R2033 T7412 T7397 nsubj data,demonstrated
R2034 T7413 T7414 mark that,is
R2035 T7414 T7397 ccomp is,demonstrated
R2036 T7415 T7414 nsubj ESG1,is
R2037 T7416 T7414 acomp dispensable,is
R2038 T7417 T7416 prep for,dispensable
R2039 T7418 T7419 compound mouse,development
R2040 T7419 T7417 pobj development,for
R2041 T7420 T7419 punct ", ",development
R2042 T7421 T7422 compound germ,cell
R2043 T7422 T7423 compound cell,formation
R2044 T7423 T7419 conj formation,development
R2045 T7424 T7423 punct ", ",formation
R2046 T7425 T7423 cc and,formation
R2047 T7426 T7427 compound ES,cell
R2048 T7427 T7428 compound cell,renewal
R2049 T7428 T7423 conj renewal,formation
R2050 T7429 T7428 compound self,renewal
R2051 T7430 T7428 punct -,renewal
R2052 T7431 T7397 punct .,demonstrated
R2053 T8265 T8264 cc and,Identification
R2054 T8266 T8264 conj analyses,Identification
R2055 T8267 T8264 prep of,Identification
R2056 T8268 T8269 compound BAC,clones
R2057 T8269 T8267 pobj clones,of
R2058 T8270 T8269 acl containing,clones
R2059 T8271 T8272 det the,gene
R2060 T8272 T8270 dobj gene,containing
R2061 T8273 T8272 compound mouse,gene
R2062 T8274 T8272 compound ESG1,gene
R2063 T8276 T8277 aux To,identify
R2064 T8277 T8278 advcl identify,performed
R2065 T8279 T8280 amod bacterial,chromosome
R2066 T8280 T8282 nmod chromosome,clones
R2067 T8281 T8280 amod artificial,chromosome
R2068 T8282 T8277 dobj clones,identify
R2069 T8283 T8280 punct (,chromosome
R2070 T8284 T8280 appos BAC,chromosome
R2071 T8285 T8282 punct ),clones
R2072 T8286 T8282 acl containing,clones
R2073 T8287 T8288 compound mouse,gene
R2074 T8288 T8286 dobj gene,containing
R2075 T8289 T8288 compound ESG1,gene
R2076 T8290 T8278 punct ", ",performed
R2077 T8291 T8278 nsubj we,performed
R2078 T8292 T8293 npadvmod PCR,based
R2079 T8293 T8295 amod based,screening
R2080 T8294 T8293 punct -,based
R2081 T8295 T8278 dobj screening,performed
R2082 T8296 T8295 prep of,screening
R2083 T8297 T8298 compound mouse,pools
R2084 T8298 T8296 pobj pools,of
R2085 T8299 T8298 compound BAC,pools
R2086 T8300 T8298 compound library,pools
R2087 T8301 T8298 compound DNA,pools
R2088 T8302 T8303 punct (,Genetics
R2089 T8303 T8295 parataxis Genetics,screening
R2090 T8304 T8303 compound Research,Genetics
R2091 T8305 T8303 punct ),Genetics
R2092 T8306 T8278 advcl using,performed
R2093 T8307 T8308 det the,primers
R2094 T8308 T8306 dobj primers,using
R2095 T8309 T8310 nmod pH34,u38
R2096 T8310 T8308 nmod u38,primers
R2097 T8311 T8310 punct -,u38
R2098 T8312 T8313 punct (,3
R2099 T8313 T8310 parataxis 3,u38
R2100 T8314 T8313 dep 5,3
R2101 T8315 T8314 punct ',5
R2102 T8316 T8313 punct -,3
R2103 T8317 T8313 dep GAAGTCTGGTTCCTTGGCAGG,3
R2104 T8318 T8313 punct -,3
R2105 T8319 T8313 punct ',3
R2106 T8320 T8313 punct ),3
R2107 T8321 T8310 cc and,u38
R2108 T8322 T8323 compound pH34,L394
R2109 T8323 T8310 conj L394,u38
R2110 T8324 T8323 punct -,L394
R2111 T8325 T8326 punct (,3
R2112 T8326 T8323 parataxis 3,L394
R2113 T8327 T8326 dep 5,3
R2114 T8328 T8327 punct ',5
R2115 T8329 T8326 punct -,3
R2116 T8330 T8326 dep ACTCGATACACTGGCCTAGC,3
R2117 T8331 T8326 punct -,3
R2118 T8332 T8326 punct ',3
R2119 T8333 T8326 punct ),3
R2120 T8334 T8278 punct .,performed
R2121 T8336 T8337 prep Following,performed
R2122 T8338 T8339 compound restriction,enzyme
R2123 T8339 T8340 compound enzyme,digestion
R2124 T8340 T8336 pobj digestion,Following
R2125 T8341 T8337 punct ", ",performed
R2126 T8342 T8337 nsubj we,performed
R2127 T8343 T8344 compound Southern,blot
R2128 T8344 T8345 compound blot,analyses
R2129 T8345 T8337 dobj analyses,performed
R2130 T8346 T8345 prep of,analyses
R2131 T8347 T8348 compound BAC,clones
R2132 T8348 T8346 pobj clones,of
R2133 T8349 T8350 mark as,described
R2134 T8350 T8337 advcl described,performed
R2135 T8351 T8352 punct [,20
R2136 T8352 T8350 parataxis 20,described
R2137 T8353 T8352 punct ],20
R2138 T8354 T8350 advcl using,described
R2139 T8355 T8356 det the,probes
R2140 T8356 T8354 dobj probes,using
R2141 T8357 T8358 nmod pH34,U258
R2142 T8358 T8356 nmod U258,probes
R2143 T8359 T8358 punct -,U258
R2144 T8360 T8361 punct (,CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2145 T8361 T8358 parataxis CTCGAGTGTACAGTCAAGTGGTTGCTGGGA,U258
R2146 T8362 T8361 nummod 5,CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2147 T8363 T8362 punct ',5
R2148 T8364 T8361 punct -,CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2149 T8365 T8361 punct -,CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2150 T8366 T8361 nummod 3,CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2151 T8367 T8361 punct ',CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2152 T8368 T8361 punct ),CTCGAGTGTACAGTCAAGTGGTTGCTGGGA
R2153 T8369 T8358 punct ", ",U258
R2154 T8370 T8371 compound pH34,U65
R2155 T8371 T8358 conj U65,U258
R2156 T8372 T8371 punct -,U65
R2157 T8373 T8374 punct (,GTGACCCTCGTGACCCGTAA
R2158 T8374 T8371 parataxis GTGACCCTCGTGACCCGTAA,U65
R2159 T8375 T8374 nummod 5,GTGACCCTCGTGACCCGTAA
R2160 T8376 T8375 punct ',5
R2161 T8377 T8374 punct -,GTGACCCTCGTGACCCGTAA
R2162 T8378 T8374 punct -,GTGACCCTCGTGACCCGTAA
R2163 T8379 T8374 nummod 3,GTGACCCTCGTGACCCGTAA
R2164 T8380 T8374 punct ',GTGACCCTCGTGACCCGTAA
R2165 T8381 T8374 punct ),GTGACCCTCGTGACCCGTAA
R2166 T8382 T8371 punct ", ",U65
R2167 T8383 T8384 compound pH34,intron1L
R2168 T8384 T8371 conj intron1L,U65
R2169 T8385 T8384 punct -,intron1L
R2170 T8386 T8387 punct (,CTGCGTGAGAGAAACACCAAACAGGC
R2171 T8387 T8384 parataxis CTGCGTGAGAGAAACACCAAACAGGC,intron1L
R2172 T8388 T8387 nummod 5,CTGCGTGAGAGAAACACCAAACAGGC
R2173 T8389 T8388 punct ',5
R2174 T8390 T8387 punct -,CTGCGTGAGAGAAACACCAAACAGGC
R2175 T8391 T8387 punct -,CTGCGTGAGAGAAACACCAAACAGGC
R2176 T8392 T8387 nummod 3,CTGCGTGAGAGAAACACCAAACAGGC
R2177 T8393 T8387 punct ',CTGCGTGAGAGAAACACCAAACAGGC
R2178 T8394 T8387 punct ),CTGCGTGAGAGAAACACCAAACAGGC
R2179 T8395 T8384 punct ", ",intron1L
R2180 T8396 T8397 compound pH34,L545
R2181 T8397 T8384 conj L545,intron1L
R2182 T8398 T8397 punct -,L545
R2183 T8399 T8400 punct (,TGTGAATGGGAAGGTTACCACTCT
R2184 T8400 T8397 parataxis TGTGAATGGGAAGGTTACCACTCT,L545
R2185 T8401 T8400 nummod 5,TGTGAATGGGAAGGTTACCACTCT
R2186 T8402 T8401 punct ',5
R2187 T8403 T8400 punct -,TGTGAATGGGAAGGTTACCACTCT
R2188 T8404 T8400 punct -,TGTGAATGGGAAGGTTACCACTCT
R2189 T8405 T8400 nummod 3,TGTGAATGGGAAGGTTACCACTCT
R2190 T8406 T8400 punct ',TGTGAATGGGAAGGTTACCACTCT
R2191 T8407 T8400 punct ),TGTGAATGGGAAGGTTACCACTCT
R2192 T8408 T8397 cc and,L545
R2193 T8409 T8410 compound pH34,SCL1
R2194 T8410 T8397 conj SCL1,L545
R2195 T8411 T8410 punct -,SCL1
R2196 T8412 T8413 punct (,GCCCTCTTCTGGTTTGTCTCGAAAT
R2197 T8413 T8410 parataxis GCCCTCTTCTGGTTTGTCTCGAAAT,SCL1
R2198 T8414 T8413 nummod 5,GCCCTCTTCTGGTTTGTCTCGAAAT
R2199 T8415 T8414 punct ',5
R2200 T8416 T8413 punct -,GCCCTCTTCTGGTTTGTCTCGAAAT
R2201 T8417 T8413 punct -,GCCCTCTTCTGGTTTGTCTCGAAAT
R2202 T8418 T8413 nummod 3,GCCCTCTTCTGGTTTGTCTCGAAAT
R2203 T8419 T8413 punct ',GCCCTCTTCTGGTTTGTCTCGAAAT
R2204 T8420 T8413 punct ),GCCCTCTTCTGGTTTGTCTCGAAAT
R2205 T8421 T8337 punct .,performed
R2206 T8423 T8424 nsubj Hybridization,revealed
R2207 T8425 T8423 prep with,Hybridization
R2208 T8426 T8427 det these,probes
R2209 T8427 T8425 pobj probes,with
R2210 T8428 T8424 dobj bands,revealed
R2211 T8429 T8428 acl containing,bands
R2212 T8430 T8431 preconj either,gene
R2213 T8431 T8429 dobj gene,containing
R2214 T8432 T8431 det the,gene
R2215 T8433 T8431 compound ESG1,gene
R2216 T8434 T8431 cc or,gene
R2217 T8435 T8431 conj pseudogenes,gene
R2218 T8436 T8424 punct .,revealed
R2219 T8438 T8439 aux To,sequence
R2220 T8439 T8440 advcl sequence,subcloned
R2221 T8441 T8442 det the,region
R2222 T8442 T8439 dobj region,sequence
R2223 T8443 T8442 acl containing,region
R2224 T8444 T8445 det the,gene
R2225 T8445 T8443 dobj gene,containing
R2226 T8446 T8445 compound mouse,gene
R2227 T8447 T8445 compound ESG1,gene
R2228 T8448 T8445 cc and,gene
R2229 T8449 T8450 det the,region
R2230 T8450 T8445 conj region,gene
R2231 T8451 T8450 nummod 3,region
R2232 T8452 T8451 punct ',3
R2233 T8453 T8450 compound flanking,region
R2234 T8454 T8440 punct ", ",subcloned
R2235 T8455 T8440 nsubj we,subcloned
R2236 T8456 T8457 det a,fragment
R2237 T8457 T8440 dobj fragment,subcloned
R2238 T8458 T8459 punct ~,15
R2239 T8459 T8460 nummod 15,kbp
R2240 T8460 T8457 compound kbp,fragment
R2241 T8461 T8462 compound XhoI,SalI
R2242 T8462 T8457 compound SalI,fragment
R2243 T8463 T8462 punct -,SalI
R2244 T8464 T8440 prep into,subcloned
R2245 T8465 T8466 det the,vector
R2246 T8466 T8464 pobj vector,into
R2247 T8467 T8466 nmod pZERO,vector
R2248 T8468 T8467 punct -,pZERO
R2249 T8469 T8467 nummod 2,pZERO
R2250 T8470 T8471 punct (,Invitrogen
R2251 T8471 T8466 parataxis Invitrogen,vector
R2252 T8472 T8471 punct ),Invitrogen
R2253 T8473 T8440 punct .,subcloned
R2254 T8475 T8476 npadvmod HindIII,digested
R2255 T8476 T8481 amod digested,fragments
R2256 T8477 T8475 punct -,HindIII
R2257 T8478 T8475 cc or,HindIII
R2258 T8479 T8475 conj EcoRI,HindIII
R2259 T8480 T8476 punct -,digested
R2260 T8481 T8482 nsubjpass fragments,cloned
R2261 T8483 T8481 prep of,fragments
R2262 T8484 T8485 det this,vector
R2263 T8485 T8483 pobj vector,of
R2264 T8486 T8482 auxpass were,cloned
R2265 T8487 T8482 advmod then,cloned
R2266 T8488 T8482 prep into,cloned
R2267 T8489 T8490 compound pBluescript,KS
R2268 T8490 T8488 pobj KS,into
R2269 T8491 T8490 punct (,KS
R2270 T8492 T8490 punct -,KS
R2271 T8493 T8490 punct ),KS
R2272 T8494 T8482 prep for,cloned
R2273 T8495 T8494 pobj sequencing,for
R2274 T8496 T8482 punct .,cloned
R2275 T8498 T8499 aux To,sequence
R2276 T8499 T8500 advcl sequence,cloned
R2277 T8501 T8502 det the,pseudogene
R2278 T8502 T8499 dobj pseudogene,sequence
R2279 T8503 T8502 compound ESG1,pseudogene
R2280 T8504 T8502 cc and,pseudogene
R2281 T8505 T8506 det the,region
R2282 T8506 T8502 conj region,pseudogene
R2283 T8507 T8506 nummod 3,region
R2284 T8508 T8507 punct ',3
R2285 T8509 T8506 compound flanking,region
R2286 T8510 T8500 punct ", ",cloned
R2287 T8511 T8512 det an,fragment
R2288 T8512 T8500 nsubjpass fragment,cloned
R2289 T8513 T8514 nummod 8,kbp
R2290 T8514 T8512 compound kbp,fragment
R2291 T8515 T8516 compound NotI,XhoI
R2292 T8516 T8512 compound XhoI,fragment
R2293 T8517 T8516 punct /,XhoI
R2294 T8518 T8500 auxpass was,cloned
R2295 T8519 T8500 prep into,cloned
R2296 T8520 T8521 compound pBluescript,KS
R2297 T8521 T8519 pobj KS,into
R2298 T8522 T8521 punct (,KS
R2299 T8523 T8521 punct -,KS
R2300 T8524 T8521 punct ),KS
R2301 T8525 T8500 punct .,cloned
R2302 T8527 T8528 nmod BamHI,fragments
R2303 T8528 T8533 nsubjpass fragments,cloned
R2304 T8529 T8527 punct -,BamHI
R2305 T8530 T8527 cc or,BamHI
R2306 T8531 T8527 conj PstI,BamHI
R2307 T8532 T8528 punct -,fragments
R2308 T8534 T8528 prep of,fragments
R2309 T8535 T8536 det this,vector
R2310 T8536 T8534 pobj vector,of
R2311 T8537 T8533 auxpass were,cloned
R2312 T8538 T8533 advmod also,cloned
R2313 T8539 T8533 prep into,cloned
R2314 T8540 T8541 compound pBluescript,KS
R2315 T8541 T8539 pobj KS,into
R2316 T8542 T8541 punct (,KS
R2317 T8543 T8541 punct -,KS
R2318 T8544 T8541 punct ),KS
R2319 T8545 T8533 punct .,cloned
R2320 T8547 T8548 aux To,identify
R2321 T8548 T8549 advcl identify,used
R2322 T8550 T8551 det the,sequence
R2323 T8551 T8548 dobj sequence,identify
R2324 T8552 T8551 acl containing,sequence
R2325 T8553 T8554 det the,regions
R2326 T8554 T8552 dobj regions,containing
R2327 T8555 T8554 nummod 5,regions
R2328 T8556 T8555 punct ',5
R2329 T8557 T8554 compound flanking,regions
R2330 T8558 T8554 prep of,regions
R2331 T8559 T8560 det the,gene
R2332 T8560 T8558 pobj gene,of
R2333 T8561 T8560 compound ESG1,gene
R2334 T8562 T8560 cc and,gene
R2335 T8563 T8564 det the,pseudogenes
R2336 T8564 T8560 conj pseudogenes,gene
R2337 T8565 T8564 amod related,pseudogenes
R2338 T8566 T8549 punct ", ",used
R2339 T8567 T8549 nsubj we,used
R2340 T8568 T8569 det a,kit
R2341 T8569 T8549 dobj kit,used
R2342 T8570 T8569 compound TOPO,kit
R2343 T8571 T8569 compound walker,kit
R2344 T8572 T8573 punct (,Invitrogen
R2345 T8573 T8569 parataxis Invitrogen,kit
R2346 T8574 T8573 punct ),Invitrogen
R2347 T8575 T8549 prep with,used
R2348 T8576 T8577 det the,primers
R2349 T8577 T8575 pobj primers,with
R2350 T8578 T8579 nmod pH34,T2L
R2351 T8579 T8577 nmod T2L,primers
R2352 T8580 T8579 punct -,T2L
R2353 T8581 T8582 punct (,3
R2354 T8582 T8579 parataxis 3,T2L
R2355 T8583 T8582 dep 5,3
R2356 T8584 T8583 punct ',5
R2357 T8585 T8582 punct -,3
R2358 T8586 T8582 dep ACTAGTCGCAGCAGGGATCCAGGAATATCT,3
R2359 T8587 T8582 punct -,3
R2360 T8588 T8582 punct ',3
R2361 T8589 T8582 punct ),3
R2362 T8590 T8579 cc and,T2L
R2363 T8591 T8592 compound pH34,L394
R2364 T8592 T8579 conj L394,T2L
R2365 T8593 T8592 punct -,L394
R2366 T8594 T8549 punct .,used
R2367 T8596 T8597 det The,sequence
R2368 T8597 T8599 nsubjpass sequence,cloned
R2369 T8598 T8597 amod resulting,sequence
R2370 T8600 T8599 auxpass was,cloned
R2371 T8601 T8599 prep into,cloned
R2372 T8602 T8601 pobj pCR2.1,into
R2373 T8603 T8604 punct (,Invitrogen
R2374 T8604 T8602 parataxis Invitrogen,pCR2.1
R2375 T8605 T8604 punct ),Invitrogen
R2376 T8606 T8599 punct .,cloned
R2377 T8608 T8609 nsubj We,obtained
R2378 T8609 T8610 ccomp obtained,cloned
R2379 T8611 T8612 det a,band
R2380 T8612 T8609 dobj band,obtained
R2381 T8613 T8614 punct ~,6
R2382 T8614 T8615 nummod 6,kbp
R2383 T8615 T8612 compound kbp,band
R2384 T8616 T8609 prep from,obtained
R2385 T8617 T8618 det the,library
R2386 T8618 T8616 pobj library,from
R2387 T8619 T8620 npadvmod NsiI,digensted
R2388 T8620 T8618 amod digensted,library
R2389 T8621 T8620 punct -,digensted
R2390 T8622 T8610 punct ;,cloned
R2391 T8623 T8624 npadvmod XbaI,digested
R2392 T8624 T8636 amod digested,fragments
R2393 T8625 T8623 punct -,XbaI
R2394 T8626 T8623 punct ", ",XbaI
R2395 T8627 T8623 conj SpeI,XbaI
R2396 T8628 T8627 punct -,SpeI
R2397 T8629 T8627 punct ", ",SpeI
R2398 T8630 T8627 conj EcoRI,SpeI
R2399 T8631 T8630 punct -,EcoRI
R2400 T8632 T8630 punct ", ",EcoRI
R2401 T8633 T8630 cc and,EcoRI
R2402 T8634 T8630 conj PstI,EcoRI
R2403 T8635 T8624 punct -,digested
R2404 T8636 T8610 nsubjpass fragments,cloned
R2405 T8637 T8636 prep of,fragments
R2406 T8638 T8639 det this,band
R2407 T8639 T8637 pobj band,of
R2408 T8640 T8610 auxpass were,cloned
R2409 T8641 T8610 prep into,cloned
R2410 T8642 T8643 compound pBluescript,KS
R2411 T8643 T8641 pobj KS,into
R2412 T8644 T8643 punct (,KS
R2413 T8645 T8643 punct -,KS
R2414 T8646 T8643 punct ),KS
R2415 T8647 T8610 prep for,cloned
R2416 T8648 T8647 pobj sequencing,for
R2417 T8649 T8610 punct .,cloned
R2418 T8651 T8652 det This,fragment
R2419 T8652 T8653 nsubj fragment,contained
R2420 T8654 T8655 det the,region
R2421 T8655 T8653 dobj region,contained
R2422 T8656 T8655 nummod 5,region
R2423 T8657 T8656 punct ',5
R2424 T8658 T8655 compound flanking,region
R2425 T8659 T8655 prep of,region
R2426 T8660 T8661 det the,gene
R2427 T8661 T8659 pobj gene,of
R2428 T8662 T8661 compound ESG1,gene
R2429 T8663 T8653 punct .,contained
R2430 T8665 T8666 det A,fragment
R2431 T8666 T8670 nsubjpass fragment,cloned
R2432 T8667 T8668 punct ~,3
R2433 T8668 T8669 nummod 3,kbp
R2434 T8669 T8666 compound kbp,fragment
R2435 T8671 T8666 punct ", ",fragment
R2436 T8672 T8666 acl obtained,fragment
R2437 T8673 T8672 prep from,obtained
R2438 T8674 T8675 det the,library
R2439 T8675 T8673 pobj library,from
R2440 T8676 T8677 npadvmod SacI,digested
R2441 T8677 T8675 amod digested,library
R2442 T8678 T8677 punct -,digested
R2443 T8679 T8670 punct ", ",cloned
R2444 T8680 T8670 auxpass was,cloned
R2445 T8681 T8670 prep into,cloned
R2446 T8682 T8681 pobj pCR2.1,into
R2447 T8683 T8670 prep for,cloned
R2448 T8684 T8683 pobj sequencing,for
R2449 T8685 T8670 punct .,cloned
R2450 T8687 T8688 det This,fragment
R2451 T8688 T8689 nsubjpass fragment,contained
R2452 T8690 T8689 auxpass was,contained
R2453 T8691 T8692 det the,region
R2454 T8692 T8689 dobj region,contained
R2455 T8693 T8692 nummod 5,region
R2456 T8694 T8693 punct ',5
R2457 T8695 T8692 acl flanking,region
R2458 T8696 T8697 det the,pseudogene
R2459 T8697 T8695 dobj pseudogene,flanking
R2460 T8698 T8689 punct .,contained
R2461 T9172 T9171 prep of,Construction
R2462 T9173 T9174 compound ESG1,vectors
R2463 T9174 T9172 pobj vectors,of
R2464 T9175 T9174 compound targeting,vectors
R2465 T9177 T9178 nsubj We,replaced
R2466 T9179 T9178 dobj all,replaced
R2467 T9180 T9179 prep of,all
R2468 T9181 T9182 det the,exons
R2469 T9182 T9180 pobj exons,of
R2470 T9183 T9182 compound ESG1,exons
R2471 T9184 T9178 prep with,replaced
R2472 T9185 T9186 nummod two,vectors
R2473 T9186 T9184 pobj vectors,with
R2474 T9187 T9186 compound targeting,vectors
R2475 T9188 T9186 acl containing,vectors
R2476 T9189 T9190 preconj either,cassette
R2477 T9190 T9188 dobj cassette,containing
R2478 T9191 T9190 det an,cassette
R2479 T9192 T9193 compound IRES,geo
R2480 T9193 T9190 compound geo,cassette
R2481 T9194 T9193 punct -,geo
R2482 T9195 T9193 compound β,geo
R2483 T9196 T9193 punct -,geo
R2484 T9197 T9198 punct [,21
R2485 T9198 T9190 parataxis 21,cassette
R2486 T9199 T9198 punct ],21
R2487 T9200 T9190 cc or,cassette
R2488 T9201 T9202 det an,HygR
R2489 T9202 T9205 compound HygR,cassette
R2490 T9203 T9202 compound IRES,HygR
R2491 T9204 T9202 punct -,HygR
R2492 T9205 T9190 conj cassette,cassette
R2493 T9206 T9178 prep by,replaced
R2494 T9207 T9208 compound promoter,trap
R2495 T9208 T9209 compound trap,selection
R2496 T9209 T9206 pobj selection,by
R2497 T9210 T9178 punct .,replaced
R2498 T9212 T9213 nsubj We,amplified
R2499 T9214 T9215 det the,arm
R2500 T9215 T9213 dobj arm,amplified
R2501 T9216 T9215 nummod 5,arm
R2502 T9217 T9216 punct ',5
R2503 T9218 T9219 punct (,kbp
R2504 T9219 T9215 parataxis kbp,arm
R2505 T9220 T9219 nummod 1.8,kbp
R2506 T9221 T9219 punct ),kbp
R2507 T9222 T9213 advcl using,amplified
R2508 T9223 T9224 compound KOD,plus
R2509 T9224 T9222 dobj plus,using
R2510 T9225 T9226 punct (,TOYOBO
R2511 T9226 T9224 parataxis TOYOBO,plus
R2512 T9227 T9226 punct ),TOYOBO
R2513 T9228 T9224 prep with,plus
R2514 T9229 T9230 det the,primers
R2515 T9230 T9228 pobj primers,with
R2516 T9231 T9232 nmod pH34,U
R2517 T9232 T9230 nmod U,primers
R2518 T9233 T9232 punct -,U
R2519 T9234 T9232 nmod targetpair5,U
R2520 T9235 T9232 punct -,U
R2521 T9236 T9237 punct (,CCGCGGAAAGTCAAGAGATTGGGTGG
R2522 T9237 T9232 parataxis CCGCGGAAAGTCAAGAGATTGGGTGG,U
R2523 T9238 T9237 nummod 5,CCGCGGAAAGTCAAGAGATTGGGTGG
R2524 T9239 T9238 punct ',5
R2525 T9240 T9237 punct -,CCGCGGAAAGTCAAGAGATTGGGTGG
R2526 T9241 T9237 punct -,CCGCGGAAAGTCAAGAGATTGGGTGG
R2527 T9242 T9237 nummod 3,CCGCGGAAAGTCAAGAGATTGGGTGG
R2528 T9243 T9237 punct ',CCGCGGAAAGTCAAGAGATTGGGTGG
R2529 T9244 T9237 punct ),CCGCGGAAAGTCAAGAGATTGGGTGG
R2530 T9245 T9232 cc and,U
R2531 T9246 T9247 compound pH34,L
R2532 T9247 T9232 conj L,U
R2533 T9248 T9247 punct -,L
R2534 T9249 T9247 compound targetpair5,L
R2535 T9250 T9247 punct -,L
R2536 T9251 T9252 punct (,GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2537 T9252 T9247 parataxis GCGGCCGCCTTTACGGGTCACGAGGGTCAC,L
R2538 T9253 T9252 nummod 5,GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2539 T9254 T9253 punct ',5
R2540 T9255 T9252 punct -,GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2541 T9256 T9252 punct -,GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2542 T9257 T9252 nummod 3,GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2543 T9258 T9252 punct ',GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2544 T9259 T9252 punct ),GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2545 T9260 T9213 punct .,amplified
R2546 T9262 T9263 det The,arm
R2547 T9263 T9266 nsubjpass arm,amplified
R2548 T9264 T9263 nummod 3,arm
R2549 T9265 T9264 punct ',3
R2550 T9267 T9268 punct (,kbp
R2551 T9268 T9263 parataxis kbp,arm
R2552 T9269 T9268 nummod 5.8,kbp
R2553 T9270 T9268 punct ),kbp
R2554 T9271 T9266 auxpass was,amplified
R2555 T9272 T9266 advcl using,amplified
R2556 T9273 T9274 det the,primers
R2557 T9274 T9272 dobj primers,using
R2558 T9275 T9276 nmod pH34,U
R2559 T9276 T9274 nmod U,primers
R2560 T9277 T9276 punct -,U
R2561 T9278 T9276 nmod targetpair3,U
R2562 T9279 T9276 punct -,U
R2563 T9280 T9281 punct (,TGTGGCCAGTGTTTGGTTCTGGCGGG
R2564 T9281 T9276 parataxis TGTGGCCAGTGTTTGGTTCTGGCGGG,U
R2565 T9282 T9281 nummod 5,TGTGGCCAGTGTTTGGTTCTGGCGGG
R2566 T9283 T9282 punct ',5
R2567 T9284 T9281 punct -,TGTGGCCAGTGTTTGGTTCTGGCGGG
R2568 T9285 T9281 punct -,TGTGGCCAGTGTTTGGTTCTGGCGGG
R2569 T9286 T9281 nummod 3,TGTGGCCAGTGTTTGGTTCTGGCGGG
R2570 T9287 T9281 punct ',TGTGGCCAGTGTTTGGTTCTGGCGGG
R2571 T9288 T9281 punct ),TGTGGCCAGTGTTTGGTTCTGGCGGG
R2572 T9289 T9276 cc and,U
R2573 T9290 T9291 compound pH34,L
R2574 T9291 T9276 conj L,U
R2575 T9292 T9291 punct -,L
R2576 T9293 T9291 compound targetpair3,L
R2577 T9294 T9291 punct -,L
R2578 T9295 T9296 punct (,CTCGAGGACTCGCCATTCTAGCCAAG
R2579 T9296 T9291 parataxis CTCGAGGACTCGCCATTCTAGCCAAG,L
R2580 T9297 T9296 nummod 5,CTCGAGGACTCGCCATTCTAGCCAAG
R2581 T9298 T9297 punct ',5
R2582 T9299 T9296 punct -,CTCGAGGACTCGCCATTCTAGCCAAG
R2583 T9300 T9296 punct -,CTCGAGGACTCGCCATTCTAGCCAAG
R2584 T9301 T9296 nummod 3,CTCGAGGACTCGCCATTCTAGCCAAG
R2585 T9302 T9296 punct ',CTCGAGGACTCGCCATTCTAGCCAAG
R2586 T9303 T9296 punct ),CTCGAGGACTCGCCATTCTAGCCAAG
R2587 T9304 T9266 punct .,amplified
R2588 T9306 T9307 det The,cassettes
R2589 T9307 T9315 nsubjpass cassettes,ligated
R2590 T9308 T9309 nmod IRES,geo
R2591 T9309 T9307 nmod geo,cassettes
R2592 T9310 T9309 nmod β,geo
R2593 T9311 T9309 punct -,geo
R2594 T9312 T9309 cc or,geo
R2595 T9313 T9314 compound IRES,HygR
R2596 T9314 T9309 conj HygR,geo
R2597 T9316 T9315 auxpass were,ligated
R2598 T9317 T9315 prep in,ligated
R2599 T9318 T9317 prep between,in
R2600 T9319 T9320 det the,fragments
R2601 T9320 T9318 pobj fragments,between
R2602 T9321 T9320 nummod two,fragments
R2603 T9322 T9320 compound PCR,fragments
R2604 T9323 T9315 punct .,ligated
R2605 T9325 T9326 det The,cassette
R2606 T9326 T9330 nsubjpass cassette,placed
R2607 T9327 T9328 compound diphtheria,A
R2608 T9328 T9326 compound A,cassette
R2609 T9329 T9328 compound toxin,A
R2610 T9331 T9330 auxpass was,placed
R2611 T9332 T9330 advmod downstream,placed
R2612 T9333 T9332 prep of,downstream
R2613 T9334 T9335 det the,arm
R2614 T9335 T9333 pobj arm,of
R2615 T9336 T9335 nummod 3,arm
R2616 T9337 T9336 punct ',3
R2617 T9338 T9330 punct .,placed
R2618 T9340 T9341 prep After,electroporated
R2619 T9342 T9340 pobj linearization,After
R2620 T9343 T9342 prep with,linearization
R2621 T9344 T9343 pobj SacII,with
R2622 T9345 T9341 punct ", ",electroporated
R2623 T9346 T9347 det these,vectors
R2624 T9347 T9341 nsubjpass vectors,electroporated
R2625 T9348 T9347 compound targeting,vectors
R2626 T9349 T9341 auxpass were,electroporated
R2627 T9350 T9341 prep into,electroporated
R2628 T9351 T9352 quantmod 2.0,107
R2629 T9352 T9354 nummod 107,cells
R2630 T9353 T9352 punct ×,107
R2631 T9354 T9350 pobj cells,into
R2632 T9355 T9354 compound RF8,cells
R2633 T9356 T9354 compound ES,cells
R2634 T9357 T9358 punct [,22
R2635 T9358 T9354 parataxis 22,cells
R2636 T9359 T9358 punct ],22
R2637 T9360 T9341 advcl using,electroporated
R2638 T9361 T9362 det a,pulser
R2639 T9362 T9360 dobj pulser,using
R2640 T9363 T9362 compound Gene,pulser
R2641 T9364 T9365 punct (,BIORAD
R2642 T9365 T9362 parataxis BIORAD,pulser
R2643 T9366 T9365 punct ),BIORAD
R2644 T9367 T9341 punct .,electroporated
R2645 T9369 T9370 amod Transfected,cells
R2646 T9370 T9371 nsubjpass cells,selected
R2647 T9372 T9371 auxpass were,selected
R2648 T9373 T9371 prep with,selected
R2649 T9374 T9375 nummod 250,μg
R2650 T9375 T9376 nmod μg,G418
R2651 T9376 T9373 pobj G418,with
R2652 T9377 T9378 punct /,mL
R2653 T9378 T9375 prep mL,μg
R2654 T9379 T9376 cc or,G418
R2655 T9380 T9381 nummod 100,μg
R2656 T9381 T9382 nmod μg,B
R2657 T9382 T9376 conj B,G418
R2658 T9383 T9384 punct /,mL
R2659 T9384 T9381 prep mL,μg
R2660 T9385 T9382 compound hygromycin,B
R2661 T9386 T9371 punct ", ",selected
R2662 T9387 T9371 advmod respectively,selected
R2663 T9388 T9371 punct .,selected
R2664 T9390 T9391 amod Genomic,DNA
R2665 T9391 T9392 nsubjpass DNA,screened
R2666 T9393 T9391 prep from,DNA
R2667 T9394 T9395 npadvmod G418,resistant
R2668 T9395 T9401 amod resistant,colonies
R2669 T9396 T9394 punct -,G418
R2670 T9397 T9394 cc or,G418
R2671 T9398 T9399 compound hygromycin,B
R2672 T9399 T9394 conj B,G418
R2673 T9400 T9395 punct -,resistant
R2674 T9401 T9393 pobj colonies,from
R2675 T9402 T9392 auxpass was,screened
R2676 T9403 T9392 prep for,screened
R2677 T9404 T9405 amod homologous,recombination
R2678 T9405 T9403 pobj recombination,for
R2679 T9406 T9392 agent by,screened
R2680 T9407 T9408 compound Southern,blotting
R2681 T9408 T9406 pobj blotting,by
R2682 T9409 T9392 punct .,screened
R2683 T9832 T9833 compound Southern,blot
R2684 T9833 T9834 compound blot,screening
R2685 T9835 T9834 prep for,screening
R2686 T9836 T9837 amod homologous,recombination
R2687 T9837 T9835 pobj recombination,for
R2688 T9839 T9840 nmod ES,cells
R2689 T9840 T9841 nmod cells,DNA
R2690 T9841 T9843 nsubjpass DNA,extracted
R2691 T9842 T9841 amod genomic,DNA
R2692 T9844 T9843 auxpass was,extracted
R2693 T9845 T9843 prep with,extracted
R2694 T9846 T9847 nmod PUREGENE,Solution
R2695 T9847 T9845 pobj Solution,with
R2696 T9848 T9846 punct ™,PUREGENE
R2697 T9849 T9850 compound Cell,Lysis
R2698 T9850 T9847 compound Lysis,Solution
R2699 T9851 T9852 punct (,systems
R2700 T9852 T9847 parataxis systems,Solution
R2701 T9853 T9852 compound Gentra,systems
R2702 T9854 T9852 punct ),systems
R2703 T9855 T9843 punct .,extracted
R2704 T9857 T9858 prep For,digested
R2705 T9859 T9860 nummod 5,analysis
R2706 T9860 T9857 pobj analysis,For
R2707 T9861 T9859 punct ',5
R2708 T9862 T9863 compound Southern,blot
R2709 T9863 T9860 compound blot,analysis
R2710 T9864 T9858 punct ", ",digested
R2711 T9865 T9866 amod genomic,DNA
R2712 T9866 T9858 nsubjpass DNA,digested
R2713 T9867 T9858 auxpass was,digested
R2714 T9868 T9858 advmod first,digested
R2715 T9869 T9858 prep with,digested
R2716 T9870 T9869 pobj PstI,with
R2717 T9871 T9858 punct ", ",digested
R2718 T9872 T9873 advmod then,separated
R2719 T9873 T9858 conj separated,digested
R2720 T9874 T9873 prep on,separated
R2721 T9875 T9876 det an,gel
R2722 T9876 T9874 pobj gel,on
R2723 T9877 T9878 nummod 0.8,%
R2724 T9878 T9876 compound %,gel
R2725 T9879 T9876 compound agarose,gel
R2726 T9880 T9873 cc and,separated
R2727 T9881 T9873 conj transferred,separated
R2728 T9882 T9881 prep to,transferred
R2729 T9883 T9884 det a,membrane
R2730 T9884 T9882 pobj membrane,to
R2731 T9885 T9884 compound nylon,membrane
R2732 T9886 T9887 mark as,described
R2733 T9887 T9881 advcl described,transferred
R2734 T9888 T9889 punct [,20
R2735 T9889 T9887 parataxis 20,described
R2736 T9890 T9889 punct ],20
R2737 T9891 T9858 punct .,digested
R2738 T9893 T9894 det A,probe
R2739 T9894 T9899 nsubjpass probe,amplified
R2740 T9895 T9896 nummod 560,bp
R2741 T9896 T9894 nmod bp,probe
R2742 T9897 T9894 nummod 5,probe
R2743 T9898 T9897 punct ',5
R2744 T9900 T9899 auxpass was,amplified
R2745 T9901 T9899 advcl using,amplified
R2746 T9902 T9903 det the,ESG1S5
R2747 T9903 T9901 dobj ESG1S5,using
R2748 T9904 T9905 punct (,GATGGTGGTGGTGACTCAGAG
R2749 T9905 T9903 parataxis GATGGTGGTGGTGACTCAGAG,ESG1S5
R2750 T9906 T9905 nummod 5,GATGGTGGTGGTGACTCAGAG
R2751 T9907 T9906 punct ',5
R2752 T9908 T9905 punct -,GATGGTGGTGGTGACTCAGAG
R2753 T9909 T9905 punct -,GATGGTGGTGGTGACTCAGAG
R2754 T9910 T9905 nummod 3,GATGGTGGTGGTGACTCAGAG
R2755 T9911 T9905 punct ',GATGGTGGTGGTGACTCAGAG
R2756 T9912 T9905 punct ),GATGGTGGTGGTGACTCAGAG
R2757 T9913 T9903 cc and,ESG1S5
R2758 T9914 T9903 conj ESG1AS5,ESG1S5
R2759 T9915 T9901 prep as,using
R2760 T9916 T9917 punct (,primers
R2761 T9917 T9915 pobj primers,as
R2762 T9918 T9919 nummod 5,CCTCCATTGCCTCTATATCAG
R2763 T9919 T9917 nmod CCTCCATTGCCTCTATATCAG,primers
R2764 T9920 T9918 punct ',5
R2765 T9921 T9919 punct -,CCTCCATTGCCTCTATATCAG
R2766 T9922 T9919 punct -,CCTCCATTGCCTCTATATCAG
R2767 T9923 T9919 nummod 3,CCTCCATTGCCTCTATATCAG
R2768 T9924 T9919 punct ',CCTCCATTGCCTCTATATCAG
R2769 T9925 T9919 punct ),CCTCCATTGCCTCTATATCAG
R2770 T9926 T9899 punct .,amplified
R2771 T9928 T9929 det The,probe
R2772 T9929 T9930 nsubj probe,labeled
R2773 T9931 T9930 advmod specifically,labeled
R2774 T9932 T9933 det an,band
R2775 T9933 T9930 dobj band,labeled
R2776 T9934 T9935 nummod 18,kbp
R2777 T9935 T9933 compound kbp,band
R2778 T9936 T9933 prep from,band
R2779 T9937 T9938 det the,locus
R2780 T9938 T9936 pobj locus,from
R2781 T9939 T9938 nmod wild,locus
R2782 T9940 T9939 punct -,wild
R2783 T9941 T9938 compound type,locus
R2784 T9942 T9933 punct ", ",band
R2785 T9943 T9944 det a,band
R2786 T9944 T9933 conj band,band
R2787 T9945 T9946 nummod 15,kbp
R2788 T9946 T9944 compound kbp,band
R2789 T9947 T9944 prep from,band
R2790 T9948 T9949 det the,locus
R2791 T9949 T9947 pobj locus,from
R2792 T9950 T9951 compound β,geo
R2793 T9951 T9949 compound geo,locus
R2794 T9952 T9951 punct -,geo
R2795 T9953 T9944 punct ", ",band
R2796 T9954 T9944 cc and,band
R2797 T9955 T9956 det a,band
R2798 T9956 T9944 conj band,band
R2799 T9957 T9958 nummod 12,kbp
R2800 T9958 T9956 compound kbp,band
R2801 T9959 T9956 prep from,band
R2802 T9960 T9961 det the,locus
R2803 T9961 T9959 pobj locus,from
R2804 T9962 T9961 compound HygR,locus
R2805 T9963 T9930 punct .,labeled
R2806 T9965 T9966 amod Genomic,DNA
R2807 T9966 T9967 nsubjpass DNA,digested
R2808 T9968 T9967 auxpass was,digested
R2809 T9969 T9967 advmod also,digested
R2810 T9970 T9967 prep with,digested
R2811 T9971 T9970 pobj SpeI,with
R2812 T9972 T9967 prep for,digested
R2813 T9973 T9974 nummod 3,analysis
R2814 T9974 T9972 pobj analysis,for
R2815 T9975 T9973 punct ',3
R2816 T9976 T9977 compound Southern,blot
R2817 T9977 T9974 compound blot,analysis
R2818 T9978 T9967 punct .,digested
R2819 T9980 T9981 det A,probe
R2820 T9981 T9986 nsubjpass probe,amplified
R2821 T9982 T9983 nummod "1,010",bp
R2822 T9983 T9981 nmod bp,probe
R2823 T9984 T9981 nummod 3,probe
R2824 T9985 T9984 punct ',3
R2825 T9987 T9986 auxpass was,amplified
R2826 T9988 T9986 prep with,amplified
R2827 T9989 T9990 det the,primers
R2828 T9990 T9988 pobj primers,with
R2829 T9991 T9990 nmod pH34U,primers
R2830 T9992 T9991 punct -,pH34U
R2831 T9993 T9991 nummod 8000,pH34U
R2832 T9994 T9995 punct (,CCAACCAGCCAGAGTTTCAGTTAT
R2833 T9995 T9991 parataxis CCAACCAGCCAGAGTTTCAGTTAT,pH34U
R2834 T9996 T9995 nummod 5,CCAACCAGCCAGAGTTTCAGTTAT
R2835 T9997 T9996 punct ',5
R2836 T9998 T9995 punct -,CCAACCAGCCAGAGTTTCAGTTAT
R2837 T9999 T9995 punct -,CCAACCAGCCAGAGTTTCAGTTAT
R2838 T10000 T9995 nummod 3,CCAACCAGCCAGAGTTTCAGTTAT
R2839 T10001 T9995 punct ',CCAACCAGCCAGAGTTTCAGTTAT
R2840 T10002 T9995 punct ),CCAACCAGCCAGAGTTTCAGTTAT
R2841 T10003 T9991 cc and,pH34U
R2842 T10004 T9991 conj pH34L,pH34U
R2843 T10005 T10004 punct -,pH34L
R2844 T10006 T10004 nummod 9000,pH34L
R2845 T10007 T10008 punct (,GATAAGCTGCTGCCAAAAGACAAG
R2846 T10008 T10004 parataxis GATAAGCTGCTGCCAAAAGACAAG,pH34L
R2847 T10009 T10008 nummod 5,GATAAGCTGCTGCCAAAAGACAAG
R2848 T10010 T10009 punct ',5
R2849 T10011 T10008 punct -,GATAAGCTGCTGCCAAAAGACAAG
R2850 T10012 T10008 punct -,GATAAGCTGCTGCCAAAAGACAAG
R2851 T10013 T10008 nummod 3,GATAAGCTGCTGCCAAAAGACAAG
R2852 T10014 T10008 punct ',GATAAGCTGCTGCCAAAAGACAAG
R2853 T10015 T10008 punct ),GATAAGCTGCTGCCAAAAGACAAG
R2854 T10016 T9986 punct .,amplified
R2855 T10018 T10019 det The,probe
R2856 T10019 T10020 nsubj probe,hybridized
R2857 T10021 T10020 prep to,hybridized
R2858 T10022 T10023 det an,band
R2859 T10023 T10021 pobj band,to
R2860 T10024 T10025 nummod 11.5,kbp
R2861 T10025 T10023 compound kbp,band
R2862 T10026 T10023 prep from,band
R2863 T10027 T10028 det the,locus
R2864 T10028 T10026 pobj locus,from
R2865 T10029 T10030 amod wild,type
R2866 T10030 T10028 compound type,locus
R2867 T10031 T10030 punct -,type
R2868 T10032 T10023 punct ", ",band
R2869 T10033 T10034 det a,band
R2870 T10034 T10023 conj band,band
R2871 T10035 T10036 nummod 12.5,kbp
R2872 T10036 T10034 compound kbp,band
R2873 T10037 T10034 prep from,band
R2874 T10038 T10039 det the,locus
R2875 T10039 T10037 pobj locus,from
R2876 T10040 T10041 compound β,geo
R2877 T10041 T10039 compound geo,locus
R2878 T10042 T10041 punct -,geo
R2879 T10043 T10034 punct ", ",band
R2880 T10044 T10034 cc and,band
R2881 T10045 T10046 det a,band
R2882 T10046 T10034 conj band,band
R2883 T10047 T10048 nummod 9.5,kbp
R2884 T10048 T10046 compound kbp,band
R2885 T10049 T10046 prep from,band
R2886 T10050 T10051 det the,locus
R2887 T10051 T10049 pobj locus,from
R2888 T10052 T10051 compound HygR,locus
R2889 T10053 T10020 punct .,hybridized
R2891 T10415 T10414 prep of,Generation
R2892 T10416 T10417 amod anti-ESG1,antibodies
R2893 T10417 T10415 pobj antibodies,of
R2894 T10418 T10417 amod polyclonal,antibodies
R2895 T10420 T10421 det The,sequence
R2896 T10421 T10423 nsubjpass sequence,amplified
R2897 T10422 T10421 amod coding,sequence
R2898 T10424 T10421 prep of,sequence
R2899 T10425 T10424 pobj Esg1,of
R2900 T10426 T10423 auxpass was,amplified
R2901 T10427 T10423 agent by,amplified
R2902 T10428 T10427 pobj PCR,by
R2903 T10429 T10423 prep with,amplified
R2904 T10430 T10431 det the,primers
R2905 T10431 T10429 pobj primers,with
R2906 T10432 T10433 nmod pH34,s
R2907 T10433 T10431 nmod s,primers
R2908 T10434 T10433 punct -,s
R2909 T10435 T10433 nmod gw,s
R2910 T10436 T10433 punct -,s
R2911 T10437 T10438 punct (,AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2912 T10438 T10433 parataxis AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA,s
R2913 T10439 T10438 nummod 5,AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2914 T10440 T10439 punct ',5
R2915 T10441 T10438 punct -,AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2916 T10442 T10438 punct -,AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2917 T10443 T10438 nummod 3,AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2918 T10444 T10438 punct ',AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2919 T10445 T10438 punct ),AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2920 T10446 T10433 cc and,s
R2921 T10447 T10448 compound pH34,as
R2922 T10448 T10433 conj as,s
R2923 T10449 T10448 punct -,as
R2924 T10450 T10448 compound gw,as
R2925 T10451 T10448 punct -,as
R2926 T10452 T10453 punct (,AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2927 T10453 T10448 parataxis AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA,as
R2928 T10454 T10453 nummod 5,AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2929 T10455 T10454 punct ',5
R2930 T10456 T10453 punct -,AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2931 T10457 T10453 punct -,AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2932 T10458 T10453 nummod 3,AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2933 T10459 T10453 punct ',AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2934 T10460 T10453 punct ),AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2935 T10461 T10423 punct .,amplified
R2936 T10463 T10464 aux To,construct
R2937 T10464 T10465 advcl construct,subcloned
R2938 T10466 T10467 compound pDONR,pH34
R2939 T10467 T10464 dobj pH34,construct
R2940 T10468 T10467 punct -,pH34
R2941 T10469 T10465 punct ", ",subcloned
R2942 T10470 T10471 det the,product
R2943 T10471 T10465 nsubjpass product,subcloned
R2944 T10472 T10471 amod resulting,product
R2945 T10473 T10471 compound PCR,product
R2946 T10474 T10465 auxpass was,subcloned
R2947 T10475 T10465 prep into,subcloned
R2948 T10476 T10475 pobj pDONR201,into
R2949 T10477 T10478 punct (,Invitrogen
R2950 T10478 T10476 parataxis Invitrogen,pDONR201
R2951 T10479 T10478 punct ),Invitrogen
R2952 T10480 T10465 punct .,subcloned
R2953 T10482 T10483 compound pDONR,pH34
R2954 T10483 T10485 nsubjpass pH34,interacted
R2955 T10484 T10483 punct -,pH34
R2956 T10486 T10485 auxpass was,interacted
R2957 T10487 T10485 prep with,interacted
R2958 T10488 T10487 pobj pDEST17,with
R2959 T10489 T10490 punct (,Invitrogen
R2960 T10490 T10488 parataxis Invitrogen,pDEST17
R2961 T10491 T10490 punct ),Invitrogen
R2962 T10492 T10485 prep by,interacted
R2963 T10493 T10494 compound LR,recombination
R2964 T10494 T10492 pobj recombination,by
R2965 T10495 T10485 punct .,interacted
R2966 T10497 T10498 prep After,induced
R2967 T10499 T10497 pobj introduction,After
R2968 T10500 T10499 prep of,introduction
R2969 T10501 T10502 det the,vector
R2970 T10502 T10500 pobj vector,of
R2971 T10503 T10502 amod resulting,vector
R2972 T10504 T10502 compound expression,vector
R2973 T10505 T10506 compound pDEST17,pH34
R2974 T10506 T10502 appos pH34,vector
R2975 T10507 T10506 punct -,pH34
R2976 T10508 T10499 prep into,introduction
R2977 T10509 T10510 compound BL21,AI
R2978 T10510 T10508 pobj AI,into
R2979 T10511 T10510 punct -,AI
R2980 T10512 T10510 nmod E.,AI
R2981 T10513 T10510 nmod coli,AI
R2982 T10514 T10515 punct (,Invitrogen
R2983 T10515 T10499 parataxis Invitrogen,introduction
R2984 T10516 T10515 punct ),Invitrogen
R2985 T10517 T10498 punct ", ",induced
R2986 T10518 T10519 amod recombinant,protein
R2987 T10519 T10520 compound protein,production
R2988 T10520 T10498 nsubjpass production,induced
R2989 T10521 T10498 auxpass was,induced
R2990 T10522 T10498 prep according,induced
R2991 T10523 T10522 prep to,according
R2992 T10524 T10525 det the,manufacture
R2993 T10525 T10526 poss manufacture,protocol
R2994 T10526 T10523 pobj protocol,to
R2995 T10527 T10525 case 's,manufacture
R2996 T10528 T10498 punct .,induced
R2997 T10530 T10531 npadvmod Histidine,tagged
R2998 T10531 T10533 amod tagged,ESG1
R2999 T10532 T10531 punct -,tagged
R3000 T10533 T10534 nsubjpass ESG1,purified
R3001 T10535 T10534 auxpass was,purified
R3002 T10536 T10534 advcl using,purified
R3003 T10537 T10538 nmod Ni,agarose
R3004 T10538 T10536 dobj agarose,using
R3005 T10539 T10537 punct -,Ni
R3006 T10540 T10537 amod nitrilotriacetic,Ni
R3007 T10541 T10538 compound acid,agarose
R3008 T10542 T10543 punct (,Qiagen
R3009 T10543 T10538 parataxis Qiagen,agarose
R3010 T10544 T10543 punct ),Qiagen
R3011 T10545 T10536 prep under,using
R3012 T10546 T10547 compound denaturing,conditions
R3013 T10547 T10545 pobj conditions,under
R3014 T10548 T10536 prep in,using
R3015 T10549 T10550 det the,presence
R3016 T10550 T10548 pobj presence,in
R3017 T10551 T10550 prep of,presence
R3018 T10552 T10553 nummod 8,M
R3019 T10553 T10554 compound M,urea
R3020 T10554 T10551 pobj urea,of
R3021 T10555 T10534 punct .,purified
R3022 T10557 T10558 prep After,injected
R3023 T10559 T10557 pobj dialysis,After
R3024 T10560 T10559 prep against,dialysis
R3025 T10561 T10562 nummod 6,M
R3026 T10562 T10563 compound M,urea
R3027 T10563 T10560 pobj urea,against
R3028 T10564 T10558 punct ", ",injected
R3029 T10565 T10566 det the,proteins
R3030 T10566 T10558 nsubjpass proteins,injected
R3031 T10567 T10566 amod recombinant,proteins
R3032 T10568 T10558 auxpass were,injected
R3033 T10569 T10558 prep into,injected
R3034 T10570 T10571 nmod New,Zealand
R3035 T10571 T10572 nmod Zealand,rabbits
R3036 T10572 T10569 pobj rabbits,into
R3037 T10573 T10572 amod White,rabbits
R3038 T10574 T10575 aux to,generate
R3039 T10575 T10558 advcl generate,injected
R3040 T10576 T10577 amod anti-ESG1,antibodies
R3041 T10577 T10575 dobj antibodies,generate
R3042 T10578 T10577 amod polyclonal,antibodies
R3043 T10579 T10558 punct .,injected
R3044 T10856 T10857 compound Western,blot
R3045 T10859 T10860 prep After,separated
R3046 T10861 T10859 pobj preparation,After
R3047 T10862 T10861 prep of,preparation
R3048 T10863 T10864 compound ES,extracts
R3049 T10864 T10862 pobj extracts,of
R3050 T10865 T10864 compound cell,extracts
R3051 T10866 T10861 prep with,preparation
R3052 T10867 T10868 compound M,Per
R3053 T10868 T10866 pobj Per,with
R3054 T10869 T10868 punct -,Per
R3055 T10870 T10871 punct (,Pierce
R3056 T10871 T10868 parataxis Pierce,Per
R3057 T10872 T10871 punct ),Pierce
R3058 T10873 T10860 punct ", ",separated
R3059 T10874 T10875 amod cellular,proteins
R3060 T10875 T10860 nsubjpass proteins,separated
R3061 T10876 T10860 auxpass were,separated
R3062 T10877 T10860 prep on,separated
R3063 T10878 T10879 nmod sodium,sulfate
R3064 T10879 T10881 nmod sulfate,gels
R3065 T10880 T10879 nmod dodecyl,sulfate
R3066 T10881 T10877 pobj gels,on
R3067 T10882 T10879 punct (,sulfate
R3068 T10883 T10879 appos SDS,sulfate
R3069 T10884 T10881 punct ),gels
R3070 T10885 T10881 punct -,gels
R3071 T10886 T10887 nummod 14,%
R3072 T10887 T10881 compound %,gels
R3073 T10888 T10881 compound polyacrylamide,gels
R3074 T10889 T10860 cc and,separated
R3075 T10890 T10860 conj transferred,separated
R3076 T10891 T10890 prep to,transferred
R3077 T10892 T10893 compound nitrocellulose,membranes
R3078 T10893 T10891 pobj membranes,to
R3079 T10894 T10895 punct (,Millipore
R3080 T10895 T10890 parataxis Millipore,transferred
R3081 T10896 T10895 punct ),Millipore
R3082 T10897 T10860 punct .,separated
R3083 T10899 T10900 nsubjpass Membranes,incubated
R3084 T10901 T10900 auxpass were,incubated
R3085 T10902 T10900 prep with,incubated
R3086 T10903 T10904 nmod anti-ESG1,antibodies
R3087 T10904 T10902 pobj antibodies,with
R3088 T10905 T10906 punct (,dilution
R3089 T10906 T10903 parataxis dilution,anti-ESG1
R3090 T10907 T10908 quantmod 1,500
R3091 T10908 T10906 nummod 500,dilution
R3092 T10909 T10908 punct /,500
R3093 T10910 T10906 punct ),dilution
R3094 T10911 T10903 punct ", ",anti-ESG1
R3095 T10912 T10903 conj anti-Oct3,anti-ESG1
R3096 T10913 T10912 punct /,anti-Oct3
R3097 T10914 T10912 nummod 4,anti-Oct3
R3098 T10915 T10916 punct (,Biotechnology
R3099 T10916 T10912 parataxis Biotechnology,anti-Oct3
R3100 T10917 T10918 quantmod 1,500
R3101 T10918 T10916 dep 500,Biotechnology
R3102 T10919 T10918 punct /,500
R3103 T10920 T10916 punct ;,Biotechnology
R3104 T10921 T10916 compound Santa,Biotechnology
R3105 T10922 T10916 compound Cruz,Biotechnology
R3106 T10923 T10916 punct ),Biotechnology
R3107 T10924 T10912 punct ", ",anti-Oct3
R3108 T10925 T10912 conj anti-CDK4,anti-Oct3
R3109 T10926 T10927 punct (,Biotechnology
R3110 T10927 T10925 parataxis Biotechnology,anti-CDK4
R3111 T10928 T10929 quantmod 1,200
R3112 T10929 T10927 dep 200,Biotechnology
R3113 T10930 T10929 punct /,200
R3114 T10931 T10927 punct ;,Biotechnology
R3115 T10932 T10927 compound Santa,Biotechnology
R3116 T10933 T10927 compound Cruz,Biotechnology
R3117 T10934 T10927 punct ),Biotechnology
R3118 T10935 T10925 punct ", ",anti-CDK4
R3119 T10936 T10925 cc and,anti-CDK4
R3120 T10937 T10925 conj anti-GFP,anti-CDK4
R3121 T10938 T10939 punct (,MBL
R3122 T10939 T10937 parataxis MBL,anti-GFP
R3123 T10940 T10941 quantmod 1,1000
R3124 T10941 T10939 dep 1000,MBL
R3125 T10942 T10941 punct /,1000
R3126 T10943 T10939 punct ;,MBL
R3127 T10944 T10939 punct ),MBL
R3128 T10945 T10904 amod primary,antibodies
R3129 T10946 T10900 punct .,incubated
R3130 T10948 T10949 compound Horseradish,peroxidase
R3131 T10949 T10950 npadvmod peroxidase,conjugated
R3132 T10950 T10952 amod conjugated,immunoglobulins
R3133 T10951 T10950 punct -,conjugated
R3134 T10952 T10956 nsubjpass immunoglobulins,used
R3135 T10953 T10952 amod anti-rabbit,immunoglobulins
R3136 T10954 T10953 cc and,anti-rabbit
R3137 T10955 T10953 conj anti-mouse,anti-rabbit
R3138 T10957 T10958 punct (,Signaling
R3139 T10958 T10952 parataxis Signaling,immunoglobulins
R3140 T10959 T10960 quantmod 1,5000
R3141 T10960 T10958 dep 5000,Signaling
R3142 T10961 T10960 punct /,5000
R3143 T10962 T10958 punct ;,Signaling
R3144 T10963 T10958 compound Cell,Signaling
R3145 T10964 T10958 punct ),Signaling
R3146 T10965 T10956 auxpass were,used
R3147 T10966 T10967 aux to,detect
R3148 T10967 T10956 advcl detect,used
R3149 T10968 T10969 compound antibody,binding
R3150 T10969 T10967 dobj binding,detect
R3151 T10970 T10956 punct .,used
R3152 T10972 T10973 nsubj We,visualized
R3153 T10974 T10975 amod bound,antibody
R3154 T10975 T10973 dobj antibody,visualized
R3155 T10976 T10973 prep with,visualized
R3156 T10977 T10978 det an,System
R3157 T10978 T10976 pobj System,with
R3158 T10979 T10978 compound ECL,System
R3159 T10980 T10981 compound Western,Blotting
R3160 T10981 T10978 compound Blotting,System
R3161 T10982 T10978 compound Detection,System
R3162 T10983 T10984 punct (,Amersham
R3163 T10984 T10978 parataxis Amersham,System
R3164 T10985 T10984 punct ),Amersham
R3165 T10986 T10973 punct .,visualized
R3168 T11477 T11476 prep of,Derivation
R3169 T11478 T11479 npadvmod ESG1,deficient
R3170 T11479 T11481 amod deficient,cells
R3171 T11480 T11479 punct -,deficient
R3172 T11481 T11477 pobj cells,of
R3173 T11482 T11481 compound ES,cells
R3174 T11483 T11476 prep from,Derivation
R3175 T11484 T11483 pobj blastocysts,from
R3176 T11486 T11487 nmod Esg1,mice
R3177 T11487 T11498 nsubjpass mice,injected
R3178 T11488 T11486 punct +,Esg1
R3179 T11489 T11486 punct /,Esg1
R3180 T11490 T11486 punct -,Esg1
R3181 T11491 T11486 cc or,Esg1
R3182 T11492 T11486 conj ESG1,Esg1
R3183 T11493 T11492 punct -,ESG1
R3184 T11494 T11492 punct /,ESG1
R3185 T11495 T11492 punct -,ESG1
R3186 T11496 T11487 nmod mutant,mice
R3187 T11497 T11487 amod female,mice
R3188 T11499 T11498 auxpass were,injected
R3189 T11500 T11498 prep with,injected
R3190 T11501 T11500 pobj Tamoxifen,with
R3191 T11502 T11503 punct (,μg
R3192 T11503 T11501 parataxis μg,Tamoxifen
R3193 T11504 T11503 nummod 10,μg
R3194 T11505 T11503 punct ),μg
R3195 T11506 T11501 cc and,Tamoxifen
R3196 T11507 T11508 compound Depo,provera
R3197 T11508 T11501 conj provera,Tamoxifen
R3198 T11509 T11508 punct -,provera
R3199 T11510 T11511 punct (,mg
R3200 T11511 T11508 parataxis mg,provera
R3201 T11512 T11511 nummod 1,mg
R3202 T11513 T11511 punct ),mg
R3203 T11514 T11498 advmod subcutaneously,injected
R3204 T11515 T11498 prep on,injected
R3205 T11516 T11517 det the,day
R3206 T11517 T11515 pobj day,on
R3207 T11518 T11517 amod third,day
R3208 T11519 T11517 prep of,day
R3209 T11520 T11519 pobj pregnancy,of
R3210 T11521 T11498 punct .,injected
R3211 T11523 T11524 nummod Four,days
R3212 T11524 T11525 npadvmod days,later
R3213 T11525 T11526 advmod later,flushed
R3214 T11527 T11526 punct ", ",flushed
R3215 T11528 T11526 nsubjpass embryos,flushed
R3216 T11529 T11528 prep in,embryos
R3217 T11530 T11529 pobj diapause,in
R3218 T11531 T11526 auxpass were,flushed
R3219 T11532 T11526 prep out,flushed
R3220 T11533 T11532 prep of,out
R3221 T11534 T11535 det the,uterus
R3222 T11535 T11533 pobj uterus,of
R3223 T11536 T11526 cc and,flushed
R3224 T11537 T11526 conj cultured,flushed
R3225 T11538 T11537 prep on,cultured
R3226 T11539 T11540 compound STO,cells
R3227 T11540 T11538 pobj cells,on
R3228 T11541 T11540 compound feeder,cells
R3229 T11542 T11537 prep in,cultured
R3230 T11543 T11544 nummod four,well
R3231 T11544 T11546 compound well,plates
R3232 T11545 T11544 punct -,well
R3233 T11546 T11542 pobj plates,in
R3234 T11547 T11537 prep in,cultured
R3235 T11548 T11549 poss Dulbecco,Medium
R3236 T11549 T11547 pobj Medium,in
R3237 T11550 T11548 case 's,Dulbecco
R3238 T11551 T11549 amod Modified,Medium
R3239 T11552 T11549 compound Eagle,Medium
R3240 T11553 T11549 punct (,Medium
R3241 T11554 T11549 appos DMEM,Medium
R3242 T11555 T11549 punct ),Medium
R3243 T11556 T11549 acl supplemented,Medium
R3244 T11557 T11556 prep with,supplemented
R3245 T11558 T11559 nummod 20,%
R3246 T11559 T11560 nmod %,Serum
R3247 T11560 T11557 pobj Serum,with
R3248 T11561 T11560 amod Fetal,Serum
R3249 T11562 T11560 amod Bovine,Serum
R3250 T11563 T11564 punct (,Hyclone
R3251 T11564 T11560 parataxis Hyclone,Serum
R3252 T11565 T11564 punct ),Hyclone
R3253 T11566 T11560 punct ", ",Serum
R3254 T11567 T11568 nummod 0.1,mM
R3255 T11568 T11569 nmod mM,Acids
R3256 T11569 T11560 conj Acids,Serum
R3257 T11570 T11569 amod Non-Essential,Acids
R3258 T11571 T11569 compound Amino,Acids
R3259 T11572 T11573 punct (,Invitrogen
R3260 T11573 T11569 parataxis Invitrogen,Acids
R3261 T11574 T11573 punct ),Invitrogen
R3262 T11575 T11569 punct ", ",Acids
R3263 T11576 T11577 nummod 2,mM
R3264 T11577 T11578 compound mM,glutamine
R3265 T11578 T11569 conj glutamine,Acids
R3266 T11579 T11578 compound L,glutamine
R3267 T11580 T11578 punct -,glutamine
R3268 T11581 T11582 punct (,Invitrogen
R3269 T11582 T11578 parataxis Invitrogen,glutamine
R3270 T11583 T11582 punct ),Invitrogen
R3271 T11584 T11578 punct ", ",glutamine
R3272 T11585 T11586 nummod 50,U
R3273 T11586 T11587 nmod U,Streptomysin
R3274 T11587 T11578 conj Streptomysin,glutamine
R3275 T11588 T11589 punct /,ml
R3276 T11589 T11586 prep ml,U
R3277 T11590 T11587 compound Penicillin,Streptomysin
R3278 T11591 T11587 punct -,Streptomysin
R3279 T11592 T11593 punct (,Invitrogen
R3280 T11593 T11587 parataxis Invitrogen,Streptomysin
R3281 T11594 T11593 punct ),Invitrogen
R3282 T11595 T11587 punct ", ",Streptomysin
R3283 T11596 T11587 cc and,Streptomysin
R3284 T11597 T11598 nummod 0.11,mM
R3285 T11598 T11599 nmod mM,mercaptoethanol
R3286 T11599 T11587 conj mercaptoethanol,Streptomysin
R3287 T11600 T11599 nummod 2,mercaptoethanol
R3288 T11601 T11599 punct -,mercaptoethanol
R3289 T11602 T11603 punct (,Invitrogen
R3290 T11603 T11599 parataxis Invitrogen,mercaptoethanol
R3291 T11604 T11603 punct ),Invitrogen
R3292 T11605 T11526 punct .,flushed
R3293 T11607 T11608 prep After,harvested
R3294 T11609 T11610 nummod six,days
R3295 T11610 T11607 pobj days,After
R3296 T11611 T11608 punct ", ",harvested
R3297 T11612 T11613 det the,mass
R3298 T11613 T11608 nsubjpass mass,harvested
R3299 T11614 T11613 amod central,mass
R3300 T11615 T11613 prep of,mass
R3301 T11616 T11617 det each,explant
R3302 T11617 T11615 pobj explant,of
R3303 T11618 T11608 auxpass was,harvested
R3304 T11619 T11608 punct ", ",harvested
R3305 T11620 T11608 conj rinsed,harvested
R3306 T11621 T11620 prep in,rinsed
R3307 T11622 T11621 pobj PBS,in
R3308 T11623 T11620 punct ", ",rinsed
R3309 T11624 T11620 cc and,rinsed
R3310 T11625 T11620 conj placed,rinsed
R3311 T11626 T11625 prep in,placed
R3312 T11627 T11628 det a,drop
R3313 T11628 T11626 pobj drop,in
R3314 T11629 T11628 prep of,drop
R3315 T11630 T11629 pobj trypsin,of
R3316 T11631 T11625 prep for,placed
R3317 T11632 T11633 det a,minutes
R3318 T11633 T11631 pobj minutes,for
R3319 T11634 T11633 amod few,minutes
R3320 T11635 T11608 punct .,harvested
R3321 T11637 T11638 det The,mass
R3322 T11638 T11640 nsubjpass mass,collected
R3323 T11639 T11638 compound cell,mass
R3324 T11641 T11640 auxpass was,collected
R3325 T11642 T11640 prep with,collected
R3326 T11643 T11644 det a,pipette
R3327 T11644 T11642 pobj pipette,with
R3328 T11645 T11646 advmod finely,drawn
R3329 T11646 T11644 amod drawn,pipette
R3330 T11647 T11646 punct -,drawn
R3331 T11648 T11646 prt out,drawn
R3332 T11649 T11644 compound Pasteur,pipette
R3333 T11650 T11644 acl preloaded,pipette
R3334 T11651 T11650 prep with,preloaded
R3335 T11652 T11651 pobj medium,with
R3336 T11653 T11640 punct ", ",collected
R3337 T11654 T11640 advcl ensuring,collected
R3338 T11655 T11656 amod minimal,carryover
R3339 T11656 T11654 dobj carryover,ensuring
R3340 T11657 T11656 prep of,carryover
R3341 T11658 T11659 det the,trypsin
R3342 T11659 T11657 pobj trypsin,of
R3343 T11660 T11640 punct .,collected
R3344 T11662 T11663 det The,cells
R3345 T11663 T11664 nsubjpass cells,transfered
R3346 T11665 T11664 auxpass were,transfered
R3347 T11666 T11664 advmod gently,transfered
R3348 T11667 T11664 prep into,transfered
R3349 T11668 T11669 det a,well
R3350 T11669 T11667 pobj well,into
R3351 T11670 T11669 amod fresh,well
R3352 T11671 T11664 prep with,transfered
R3353 T11672 T11673 nummod 20,%
R3354 T11673 T11674 nmod %,medium
R3355 T11674 T11671 pobj medium,with
R3356 T11675 T11676 npadvmod FBS,containing
R3357 T11676 T11674 amod containing,medium
R3358 T11677 T11676 punct -,containing
R3359 T11678 T11664 punct .,transfered
R3360 T11680 T11681 det The,colonies
R3361 T11681 T11686 nsubjpass colonies,passaged
R3362 T11682 T11681 amod resulting,colonies
R3363 T11683 T11681 amod primary,colonies
R3364 T11684 T11681 compound ES,colonies
R3365 T11685 T11681 compound cell,colonies
R3366 T11687 T11686 auxpass were,passaged
R3367 T11688 T11686 advmod individually,passaged
R3368 T11689 T11686 prep into,passaged
R3369 T11690 T11689 pobj wells,into
R3370 T11691 T11690 prep of,wells
R3371 T11692 T11693 nummod four,well
R3372 T11693 T11695 compound well,plates
R3373 T11694 T11693 punct -,well
R3374 T11695 T11691 pobj plates,of
R3375 T11696 T11690 acl containing,wells
R3376 T11697 T11698 compound STO,layers
R3377 T11698 T11696 dobj layers,containing
R3378 T11699 T11700 compound feeder,cell
R3379 T11700 T11698 compound cell,layers
R3380 T11701 T11686 punct .,passaged
R3381 T11703 T11704 advmod Thereafter,expanded
R3382 T11705 T11704 punct ", ",expanded
R3383 T11706 T11704 nsubjpass cells,expanded
R3384 T11707 T11704 auxpass were,expanded
R3385 T11708 T11704 prep by,expanded
R3386 T11709 T11708 pobj trypsinization,by
R3387 T11710 T11709 prep of,trypsinization
R3388 T11711 T11712 det the,culture
R3389 T11712 T11710 pobj culture,of
R3390 T11713 T11712 amod entire,culture
R3391 T11714 T11704 punct .,expanded
R3392 T11885 T11886 amod Total,RNA
R3393 T11886 T11887 nsubjpass RNA,labeled
R3394 T11888 T11886 prep from,RNA
R3395 T11889 T11890 amod wild,type
R3396 T11890 T11892 compound type,cells
R3397 T11891 T11890 punct -,type
R3398 T11892 T11888 pobj cells,from
R3399 T11893 T11892 compound ES,cells
R3400 T11894 T11892 cc and,cells
R3401 T11895 T11896 nmod ESG1,cells
R3402 T11896 T11892 conj cells,cells
R3403 T11897 T11895 punct -,ESG1
R3404 T11898 T11895 punct /,ESG1
R3405 T11899 T11895 punct -,ESG1
R3406 T11900 T11896 compound ES,cells
R3407 T11901 T11887 auxpass was,labeled
R3408 T11902 T11887 prep with,labeled
R3409 T11903 T11902 pobj Cy3,with
R3410 T11904 T11903 cc and,Cy3
R3411 T11905 T11903 conj Cy5,Cy3
R3412 T11906 T11887 punct ", ",labeled
R3413 T11907 T11887 advmod respectively,labeled
R3414 T11908 T11887 punct .,labeled
R3415 T11910 T11911 det The,samples
R3416 T11911 T11912 nsubjpass samples,hybridized
R3417 T11913 T11912 auxpass were,hybridized
R3418 T11914 T11912 prep to,hybridized
R3419 T11915 T11916 det a,Microarray
R3420 T11916 T11914 pobj Microarray,to
R3421 T11917 T11916 compound Mouse,Microarray
R3422 T11918 T11916 compound Development,Microarray
R3423 T11919 T11920 punct (,Algilent
R3424 T11920 T11916 parataxis Algilent,Microarray
R3425 T11921 T11920 punct ),Algilent
R3426 T11922 T11912 prep according,hybridized
R3427 T11923 T11922 prep to,according
R3428 T11924 T11925 det the,manufacturer
R3429 T11925 T11926 poss manufacturer,protocol
R3430 T11926 T11923 pobj protocol,to
R3431 T11927 T11925 case 's,manufacturer
R3432 T11928 T11912 punct .,hybridized
R3433 T11930 T11931 nsubjpass Arrays,scanned
R3434 T11932 T11931 auxpass were,scanned
R3435 T11933 T11931 prep with,scanned
R3436 T11934 T11935 det a,System
R3437 T11935 T11933 pobj System,with
R3438 T11936 T11935 compound G2565BA,System
R3439 T11937 T11935 compound Microarray,System
R3440 T11938 T11935 compound Scanner,System
R3441 T11939 T11940 punct (,Agilent
R3442 T11940 T11935 parataxis Agilent,System
R3443 T11941 T11940 punct ),Agilent
R3444 T11942 T11931 punct .,scanned
R3445 T11944 T11945 nsubjpass Hybridization,repeated
R3446 T11946 T11945 auxpass was,repeated
R3447 T11947 T11945 prep with,repeated
R3448 T11948 T11949 nummod two,clones
R3449 T11949 T11947 pobj clones,with
R3450 T11950 T11949 amod independent,clones
R3451 T11951 T11945 punct .,repeated
R3452 T11953 T11954 nsubjpass Data,analyzed
R3453 T11955 T11954 auxpass were,analyzed
R3454 T11956 T11954 prep with,analyzed
R3455 T11957 T11958 compound GeneSprings,software
R3456 T11958 T11956 pobj software,with
R3457 T11959 T11960 punct (,Genetics
R3458 T11960 T11958 parataxis Genetics,software
R3459 T11961 T11960 compound Silico,Genetics
R3460 T11962 T11960 punct ),Genetics
R3461 T11963 T11954 punct .,analyzed
R3462 T12056 T12055 acl identified,ESG1pseudogenes
R3463 T12057 T12056 agent by,identified
R3464 T12058 T12059 det a,search
R3465 T12059 T12057 pobj search,by
R3466 T12060 T12059 compound Blast,search
R3467 T12061 T12059 prep of,search
R3468 T12062 T12063 nmod mouse,databases
R3469 T12063 T12061 pobj databases,of
R3470 T12064 T12063 amod genomic,databases
R3471 T12065 T12055 punct .,ESG1pseudogenes
R3472 T12067 T12068 compound Substitution,mutations
R3473 T12068 T12069 nsubjpass mutations,indicated
R3474 T12070 T12069 auxpass are,indicated
R3475 T12071 T12069 agent by,indicated
R3476 T12072 T12073 amod black,lines
R3477 T12073 T12071 pobj lines,by
R3478 T12074 T12069 punct .,indicated
R3479 T12076 T12077 npadvmod Intron,like
R3480 T12077 T12079 amod like,sequences
R3481 T12078 T12077 punct -,like
R3482 T12079 T12081 nsubjpass sequences,indicated
R3483 T12080 T12079 compound gap,sequences
R3484 T12082 T12081 auxpass are,indicated
R3485 T12083 T12081 prep with,indicated
R3486 T12084 T12083 pobj triangles,with
R3487 T12085 T12081 punct .,indicated
R3488 T12087 T12088 amod Chromosomal,localizations
R3489 T12088 T12089 nsubjpass localizations,shown
R3490 T12090 T12089 auxpass are,shown
R3491 T12091 T12089 prep on,shown
R3492 T12092 T12093 det the,right
R3493 T12093 T12091 pobj right,on
R3494 T12094 T12089 punct .,shown
R3495 T12275 T12276 compound BAC,clones
R3496 T12276 T12277 nsubj clones,containing
R3497 T12278 T12279 det the,gene
R3498 T12279 T12277 dobj gene,containing
R3499 T12280 T12279 compound ESG1,gene
R3500 T12281 T12279 cc and,gene
R3501 T12282 T12283 det a,pseudogene
R3502 T12283 T12279 conj pseudogene,gene
R3503 T12284 T12283 compound duplication,pseudogene
R3504 T12285 T12286 punct (,PS
R3505 T12286 T12283 parataxis PS,pseudogene
R3506 T12287 T12286 punct ),PS
R3507 T12288 T12277 punct .,containing
R3508 T12290 T12291 meta A,Localization
R3509 T12292 T12290 punct ),A
R3510 T12293 T12291 prep of,Localization
R3511 T12294 T12295 det the,gene
R3512 T12295 T12293 pobj gene,of
R3513 T12296 T12295 cc and,gene
R3514 T12297 T12295 conj PS,gene
R3515 T12298 T12291 prep on,Localization
R3516 T12299 T12298 pobj chromosome,on
R3517 T12300 T12299 nummod 9,chromosome
R3518 T12301 T12291 punct .,Localization
R3519 T12303 T12304 meta B,revealed
R3520 T12305 T12303 punct ),B
R3521 T12306 T12307 compound Sequence,comparison
R3522 T12307 T12304 nsubj comparison,revealed
R3523 T12308 T12307 prep of,comparison
R3524 T12309 T12310 det the,gene
R3525 T12310 T12308 pobj gene,of
R3526 T12311 T12310 cc and,gene
R3527 T12312 T12310 conj PS,gene
R3528 T12313 T12314 mark that,are
R3529 T12314 T12304 ccomp are,revealed
R3530 T12315 T12314 nsubj these,are
R3531 T12316 T12314 acomp homologous,are
R3532 T12317 T12316 prep from,homologous
R3533 T12318 T12319 nummod eight,pairs
R3534 T12319 T12317 pobj pairs,from
R3535 T12320 T12319 compound base,pairs
R3536 T12321 T12319 advmod upstream,pairs
R3537 T12322 T12321 prep of,upstream
R3538 T12323 T12324 det the,exon
R3539 T12324 T12322 pobj exon,of
R3540 T12325 T12324 amod first,exon
R3541 T12326 T12317 prep to,from
R3542 T12327 T12328 nummod 675,bp
R3543 T12328 T12326 pobj bp,to
R3544 T12329 T12328 advmod downstream,bp
R3545 T12330 T12329 prep of,downstream
R3546 T12331 T12332 det the,exon
R3547 T12332 T12330 pobj exon,of
R3548 T12333 T12332 amod third,exon
R3549 T12334 T12304 punct .,revealed
R3550 T12336 T12337 compound Substitution,mutations
R3551 T12337 T12338 nsubjpass mutations,indicated
R3552 T12339 T12338 auxpass are,indicated
R3553 T12340 T12338 prep by,indicated
R3554 T12341 T12342 amod black,lines
R3555 T12342 T12340 pobj lines,by
R3556 T12343 T12338 punct .,indicated
R3557 T12345 T12346 det The,insertion
R3558 T12346 T12347 nsubjpass insertion,indicated
R3559 T12348 T12347 auxpass is,indicated
R3560 T12349 T12347 prep by,indicated
R3561 T12350 T12351 det an,triangle
R3562 T12351 T12349 pobj triangle,by
R3563 T12352 T12351 amod open,triangle
R3564 T12353 T12347 punct .,indicated
R3565 T12543 T12544 compound Promoter,enhancer
R3566 T12544 T12546 compound enhancer,activity
R3567 T12545 T12544 punct /,enhancer
R3568 T12547 T12546 prep of,activity
R3569 T12548 T12549 det the,gene
R3570 T12549 T12547 pobj gene,of
R3571 T12550 T12549 compound ESG1,gene
R3572 T12551 T12549 cc and,gene
R3573 T12552 T12549 conj pseudogene,gene
R3574 T12553 T12546 punct .,activity
R3575 T12555 T12556 compound DNA,fragments
R3576 T12556 T12557 nsubjpass fragments,transferred
R3577 T12558 T12556 prep of,fragments
R3578 T12559 T12560 punct ~,6
R3579 T12560 T12561 nummod 6,kbp
R3580 T12561 T12558 pobj kbp,of
R3581 T12562 T12561 acl isolated,kbp
R3582 T12563 T12562 prep from,isolated
R3583 T12564 T12565 det the,regions
R3584 T12565 T12563 pobj regions,from
R3585 T12566 T12565 nummod 5,regions
R3586 T12567 T12566 punct ',5
R3587 T12568 T12565 compound flanking,regions
R3588 T12569 T12565 prep of,regions
R3589 T12570 T12571 det the,gene
R3590 T12571 T12569 pobj gene,of
R3591 T12572 T12571 cc and,gene
R3592 T12573 T12571 conj PS,gene
R3593 T12574 T12557 auxpass were,transferred
R3594 T12575 T12557 prep into,transferred
R3595 T12576 T12577 compound luciferase,reporter
R3596 T12577 T12578 compound reporter,plasmids
R3597 T12578 T12575 pobj plasmids,into
R3598 T12579 T12557 punct .,transferred
R3599 T12581 T12582 nsubj We,introduced
R3600 T12583 T12584 det the,genes
R3601 T12584 T12582 dobj genes,introduced
R3602 T12585 T12584 compound reporter,genes
R3603 T12586 T12582 prep into,introduced
R3604 T12587 T12588 amod undifferentiated,cells
R3605 T12588 T12586 pobj cells,into
R3606 T12589 T12588 compound ES,cells
R3607 T12590 T12591 punct (,columns
R3608 T12591 T12588 parataxis columns,cells
R3609 T12592 T12591 amod open,columns
R3610 T12593 T12591 punct ),columns
R3611 T12594 T12588 punct ", ",cells
R3612 T12595 T12596 amod retinoic,acid
R3613 T12596 T12597 npadvmod acid,treated
R3614 T12597 T12599 amod treated,cells
R3615 T12598 T12597 punct -,treated
R3616 T12599 T12588 conj cells,cells
R3617 T12600 T12599 compound ES,cells
R3618 T12601 T12602 punct (,columns
R3619 T12602 T12599 parataxis columns,cells
R3620 T12603 T12602 amod grey,columns
R3621 T12604 T12602 punct ),columns
R3622 T12605 T12599 punct ", ",cells
R3623 T12606 T12599 cc and,cells
R3624 T12607 T12608 compound NIH3T3,cells
R3625 T12608 T12599 conj cells,cells
R3626 T12609 T12610 punct (,columns
R3627 T12610 T12608 parataxis columns,cells
R3628 T12611 T12610 amod closed,columns
R3629 T12612 T12610 punct ),columns
R3630 T12613 T12582 punct .,introduced
R3631 T12615 T12616 nsubj Data,represent
R3632 T12617 T12618 det the,averages
R3633 T12618 T12616 dobj averages,represent
R3634 T12619 T12618 cc and,averages
R3635 T12620 T12621 amod standard,deviations
R3636 T12621 T12618 conj deviations,averages
R3637 T12622 T12618 prep of,averages
R3638 T12623 T12624 nummod three,experiments
R3639 T12624 T12622 pobj experiments,of
R3640 T12625 T12624 amod independent,experiments
R3641 T12626 T12616 punct .,represent
R3642 T13078 T13079 amod Targeted,disruption
R3643 T13080 T13079 prep of,disruption
R3644 T13081 T13082 det the,gene
R3645 T13082 T13080 pobj gene,of
R3646 T13083 T13082 compound mouse,gene
R3647 T13084 T13082 compound ESG1,gene
R3648 T13085 T13079 punct .,disruption
R3649 T13087 T13088 meta A,strategy
R3650 T13089 T13087 punct ),A
R3651 T13090 T13088 compound Targeting,strategy
R3652 T13091 T13088 punct .,strategy
R3653 T13093 T13094 amod Homologous,regions
R3654 T13094 T13095 nsubjpass regions,indicated
R3655 T13096 T13095 auxpass are,indicated
R3656 T13097 T13095 agent by,indicated
R3657 T13098 T13099 amod thick,lines
R3658 T13099 T13097 pobj lines,by
R3659 T13100 T13095 punct .,indicated
R3660 T13102 T13103 compound Recognition,sites
R3661 T13103 T13104 nsubjpass sites,shown
R3662 T13105 T13103 prep of,sites
R3663 T13106 T13105 pobj PstI,of
R3664 T13107 T13108 punct (,P
R3665 T13108 T13106 parataxis P,PstI
R3666 T13109 T13108 punct ),P
R3667 T13110 T13106 cc and,PstI
R3668 T13111 T13106 conj SpeI,PstI
R3669 T13112 T13113 punct (,S
R3670 T13113 T13111 parataxis S,SpeI
R3671 T13114 T13113 punct ),S
R3672 T13115 T13103 punct ", ",sites
R3673 T13116 T13117 dep which,used
R3674 T13117 T13103 relcl used,sites
R3675 T13118 T13117 auxpass were,used
R3676 T13119 T13117 prep for,used
R3677 T13120 T13121 compound Southern,blot
R3678 T13121 T13122 compound blot,analyses
R3679 T13122 T13119 pobj analyses,for
R3680 T13123 T13104 punct ", ",shown
R3681 T13124 T13104 auxpass are,shown
R3682 T13125 T13104 punct .,shown
R3683 T13127 T13128 det The,A
R3684 T13128 T13133 nsubjpass A,inserted
R3685 T13129 T13128 nmod gene,A
R3686 T13130 T13128 amod encoding,A
R3687 T13131 T13128 compound diphtheria,A
R3688 T13132 T13128 compound toxin,A
R3689 T13134 T13128 punct (,A
R3690 T13135 T13128 appos DTA,A
R3691 T13136 T13133 punct ),inserted
R3692 T13137 T13133 auxpass was,inserted
R3693 T13138 T13133 prep at,inserted
R3694 T13139 T13140 det the,end
R3695 T13140 T13138 pobj end,at
R3696 T13141 T13140 nummod 3,end
R3697 T13142 T13141 punct ',3
R3698 T13143 T13140 prep of,end
R3699 T13144 T13145 det the,vectors
R3700 T13145 T13143 pobj vectors,of
R3701 T13146 T13145 compound targeting,vectors
R3702 T13147 T13148 aux to,facilitate
R3703 T13148 T13133 advcl facilitate,inserted
R3704 T13149 T13150 amod negative,selection
R3705 T13150 T13148 dobj selection,facilitate
R3706 T13151 T13133 punct .,inserted
R3707 T13153 T13154 meta B,analyses
R3708 T13155 T13153 punct ),B
R3709 T13156 T13157 compound Southern,blot
R3710 T13157 T13154 compound blot,analyses
R3711 T13158 T13154 acl confirming,analyses
R3712 T13159 T13160 amod homologous,recombination
R3713 T13160 T13158 dobj recombination,confirming
R3714 T13161 T13154 punct .,analyses
R3715 T13164 T13163 punct ", ",WT
R3716 T13165 T13166 amod wild,type
R3717 T13166 T13168 compound type,cells
R3718 T13167 T13166 punct -,type
R3719 T13168 T13163 appos cells,WT
R3720 T13169 T13168 compound ES,cells
R3721 T13170 T13163 punct ;,WT
R3722 T13171 T13163 appos β,WT
R3723 T13172 T13171 punct ", ",β
R3724 T13173 T13174 nmod β,geo
R3725 T13174 T13176 nmod geo,cells
R3726 T13175 T13174 punct -,geo
R3727 T13176 T13171 appos cells,β
R3728 T13177 T13174 punct +,geo
R3729 T13178 T13174 punct /,geo
R3730 T13179 T13174 punct -,geo
R3731 T13180 T13176 compound ES,cells
R3732 T13181 T13163 punct ;,WT
R3733 T13182 T13163 appos H,WT
R3734 T13183 T13182 punct ", ",H
R3735 T13184 T13185 nmod HygR,cells
R3736 T13185 T13182 appos cells,H
R3737 T13186 T13184 punct +,HygR
R3738 T13187 T13184 punct /,HygR
R3739 T13188 T13184 punct -,HygR
R3740 T13189 T13185 compound ES,cells
R3741 T13190 T13163 punct ;,WT
R3742 T13191 T13192 punct -,-
R3743 T13192 T13163 appos -,WT
R3744 T13193 T13192 punct /,-
R3745 T13194 T13192 punct ", ",-
R3746 T13195 T13196 npadvmod ESG1,null
R3747 T13196 T13198 amod null,cells
R3748 T13197 T13196 punct -,null
R3749 T13198 T13192 appos cells,-
R3750 T13199 T13198 compound ES,cells
R3751 T13200 T13163 punct .,WT
R3752 T13202 T13203 nsubj Numbers,indicate
R3753 T13204 T13205 compound clone,numbers
R3754 T13205 T13203 dobj numbers,indicate
R3755 T13206 T13203 punct .,indicate
R3756 T13208 T13209 meta C,analyses
R3757 T13210 T13208 punct ),C
R3758 T13211 T13212 nmod Northern,blot
R3759 T13212 T13209 nmod blot,analyses
R3760 T13213 T13214 punct (,upper
R3761 T13214 T13212 parataxis upper,blot
R3762 T13215 T13214 punct ),upper
R3763 T13216 T13212 cc and,blot
R3764 T13217 T13218 compound western,blot
R3765 T13218 T13212 conj blot,blot
R3766 T13219 T13220 punct (,lower
R3767 T13220 T13218 parataxis lower,blot
R3768 T13221 T13220 punct ),lower
R3769 T13222 T13209 prep of,analyses
R3770 T13223 T13224 amod wild,type
R3771 T13224 T13226 compound type,cells
R3772 T13225 T13224 punct -,type
R3773 T13226 T13222 pobj cells,of
R3774 T13227 T13226 compound ES,cells
R3775 T13228 T13229 punct (,WT
R3776 T13229 T13226 parataxis WT,cells
R3777 T13230 T13229 punct ),WT
R3778 T13231 T13226 punct ", ",cells
R3779 T13232 T13233 npadvmod ESG1,null
R3780 T13233 T13235 amod null,cells
R3781 T13234 T13233 punct -,null
R3782 T13235 T13226 conj cells,cells
R3783 T13236 T13235 compound ES,cells
R3784 T13237 T13238 punct (,clones
R3785 T13238 T13235 parataxis clones,cells
R3786 T13239 T13240 punct -,-
R3787 T13240 T13238 punct -,clones
R3788 T13241 T13240 punct /,-
R3789 T13242 T13238 punct ", ",clones
R3790 T13243 T13238 nummod three,clones
R3791 T13244 T13238 punct ),clones
R3792 T13245 T13235 cc and,cells
R3793 T13246 T13247 amod heterozygous,cells
R3794 T13247 T13235 conj cells,cells
R3795 T13248 T13247 compound ES,cells
R3796 T13249 T13250 punct (,-
R3797 T13250 T13247 punct -,cells
R3798 T13251 T13250 punct +,-
R3799 T13252 T13250 punct /,-
R3800 T13253 T13250 punct ),-
R3801 T13254 T13209 punct .,analyses
R3802 T13256 T13257 compound Northern,blot
R3803 T13257 T13258 nsubjpass blot,performed
R3804 T13259 T13258 auxpass was,performed
R3805 T13260 T13261 mark as,described
R3806 T13261 T13258 advcl described,performed
R3807 T13262 T13261 advmod previously,described
R3808 T13263 T13264 punct [,20
R3809 T13264 T13261 parataxis 20,described
R3810 T13265 T13264 punct ],20
R3811 T13266 T13258 punct .,performed
R3812 T13268 T13269 aux To,confirm
R3813 T13269 T13270 advcl confirm,shown
R3814 T13271 T13272 det the,loading
R3815 T13272 T13269 dobj loading,confirm
R3816 T13273 T13272 prep of,loading
R3817 T13274 T13275 amod equal,amounts
R3818 T13275 T13273 pobj amounts,of
R3819 T13276 T13275 prep of,amounts
R3820 T13277 T13276 pobj RNA,of
R3821 T13278 T13270 punct ", ",shown
R3822 T13279 T13280 compound ethidium,bromide
R3823 T13280 T13281 compound bromide,staining
R3824 T13281 T13270 nsubjpass staining,shown
R3825 T13282 T13281 prep of,staining
R3826 T13283 T13284 amod ribosomal,RNA
R3827 T13284 T13282 pobj RNA,of
R3828 T13285 T13270 auxpass is,shown
R3829 T13286 T13270 advmod also,shown
R3830 T13287 T13288 punct (,middle
R3831 T13288 T13270 parataxis middle,shown
R3832 T13289 T13288 punct ),middle
R3833 T13290 T13270 punct .,shown
R3834 T13588 T13587 prep of,Analyses
R3835 T13589 T13590 npadvmod ESG1,null
R3836 T13590 T13592 amod null,cells
R3837 T13591 T13590 punct -,null
R3838 T13592 T13588 pobj cells,of
R3839 T13593 T13592 compound ES,cells
R3840 T13594 T13587 punct .,Analyses
R3841 T13596 T13597 meta A,morphology
R3842 T13598 T13596 punct ),A
R3843 T13599 T13597 det The,morphology
R3844 T13600 T13597 prep of,morphology
R3845 T13601 T13602 npadvmod ESG1,null
R3846 T13602 T13604 amod null,colonies
R3847 T13603 T13602 punct -,null
R3848 T13604 T13600 pobj colonies,of
R3849 T13605 T13604 compound ES,colonies
R3850 T13606 T13604 compound cell,colonies
R3851 T13607 T13597 acl grown,morphology
R3852 T13608 T13607 prep on,grown
R3853 T13609 T13610 compound STO,cells
R3854 T13610 T13608 pobj cells,on
R3855 T13611 T13610 compound feeder,cells
R3856 T13612 T13597 punct .,morphology
R3857 T13614 T13615 meta B,curve
R3858 T13616 T13614 punct ),B
R3859 T13617 T13615 compound Growth,curve
R3860 T13618 T13615 prep of,curve
R3861 T13619 T13620 amod wild,type
R3862 T13620 T13622 nmod type,cells
R3863 T13621 T13620 punct -,type
R3864 T13622 T13618 pobj cells,of
R3865 T13623 T13620 punct (,type
R3866 T13624 T13620 appos WT,type
R3867 T13625 T13620 punct ),type
R3868 T13626 T13620 punct ", ",type
R3869 T13627 T13620 conj ESG1,type
R3870 T13628 T13627 punct -,ESG1
R3871 T13629 T13627 amod null,ESG1
R3872 T13630 T13627 punct (,ESG1
R3873 T13631 T13632 punct -,-
R3874 T13632 T13627 appos -,ESG1
R3875 T13633 T13632 punct /,-
R3876 T13634 T13627 punct ),ESG1
R3877 T13635 T13627 cc and,ESG1
R3878 T13636 T13627 conj heterozygous,ESG1
R3879 T13637 T13636 punct (,heterozygous
R3880 T13638 T13639 punct +,-
R3881 T13639 T13636 appos -,heterozygous
R3882 T13640 T13639 punct /,-
R3883 T13641 T13622 punct ),cells
R3884 T13642 T13622 compound ES,cells
R3885 T13643 T13615 punct .,curve
R3886 T13645 T13646 det Each,clone
R3887 T13646 T13647 nsubjpass clone,plated
R3888 T13648 T13649 punct (,cells
R3889 T13649 T13646 parataxis cells,clone
R3890 T13650 T13651 quantmod 1,104
R3891 T13651 T13649 nummod 104,cells
R3892 T13652 T13651 punct ×,104
R3893 T13653 T13654 punct /,well
R3894 T13654 T13649 prep well,cells
R3895 T13655 T13649 punct ),cells
R3896 T13656 T13647 auxpass was,plated
R3897 T13657 T13647 prep in,plated
R3898 T13658 T13659 nummod 24,well
R3899 T13659 T13661 compound well,plates
R3900 T13660 T13659 punct -,well
R3901 T13661 T13657 pobj plates,in
R3902 T13662 T13647 punct .,plated
R3903 T13664 T13665 compound Cell,numbers
R3904 T13665 T13666 nsubjpass numbers,determined
R3905 T13667 T13666 auxpass were,determined
R3906 T13668 T13666 prep with,determined
R3907 T13669 T13670 det a,counter
R3908 T13670 T13668 pobj counter,with
R3909 T13671 T13670 compound Coulter,counter
R3910 T13672 T13666 prep at,determined
R3911 T13673 T13674 nummod 2,days
R3912 T13674 T13672 pobj days,at
R3913 T13675 T13673 punct ", ",2
R3914 T13676 T13673 conj 4,2
R3915 T13677 T13676 punct ", ",4
R3916 T13678 T13676 cc and,4
R3917 T13679 T13676 conj 6,4
R3918 T13680 T13666 punct .,determined
R3919 T13682 T13683 nsubjpass Data,shown
R3920 T13684 T13682 prep of,Data
R3921 T13685 T13686 punct +,-
R3922 T13686 T13688 punct -,cells
R3923 T13687 T13686 punct /,-
R3924 T13688 T13684 pobj cells,of
R3925 T13689 T13686 cc and,-
R3926 T13690 T13691 punct -,-
R3927 T13691 T13686 conj -,-
R3928 T13692 T13691 punct /,-
R3929 T13693 T13683 auxpass are,shown
R3930 T13694 T13683 prep as,shown
R3931 T13695 T13694 pobj averages,as
R3932 T13696 T13695 cc and,averages
R3933 T13697 T13698 amod standard,deviations
R3934 T13698 T13695 conj deviations,averages
R3935 T13699 T13695 prep of,averages
R3936 T13700 T13701 nummod three,clones
R3937 T13701 T13699 pobj clones,of
R3938 T13702 T13701 amod independent,clones
R3939 T13703 T13683 punct .,shown
R3940 T13705 T13706 meta C,section
R3941 T13707 T13705 punct ),C
R3942 T13708 T13706 det A,section
R3943 T13709 T13706 prep of,section
R3944 T13710 T13709 pobj teratoma,of
R3945 T13711 T13710 acl derived,teratoma
R3946 T13712 T13711 prep from,derived
R3947 T13713 T13714 npadvmod ESG1,null
R3948 T13714 T13716 amod null,cells
R3949 T13715 T13714 punct -,null
R3950 T13716 T13712 pobj cells,from
R3951 T13717 T13716 compound ES,cells
R3952 T13718 T13719 punct (,staining
R3953 T13719 T13711 parataxis staining,derived
R3954 T13720 T13719 nmod hematoxylin,staining
R3955 T13721 T13720 cc &,hematoxylin
R3956 T13722 T13720 conj eosin,hematoxylin
R3957 T13723 T13719 punct ),staining
R3958 T13724 T13706 punct .,section
R3959 T13961 T13962 compound Gene,expression
R3960 T13962 T13963 compound expression,analyses
R3961 T13964 T13963 prep of,analyses
R3962 T13965 T13966 npadvmod ESG1,null
R3963 T13966 T13968 amod null,cells
R3964 T13967 T13966 punct -,null
R3965 T13968 T13964 pobj cells,of
R3966 T13969 T13968 compound ES,cells
R3967 T13970 T13963 punct .,analyses
R3968 T13972 T13973 meta A,analyses
R3969 T13974 T13972 punct ),A
R3970 T13975 T13973 compound DNA,analyses
R3971 T13976 T13973 compound microarray,analyses
R3972 T13977 T13973 punct .,analyses
R3973 T13979 T13980 amod Total,RNA
R3974 T13980 T13981 nsubjpass RNA,labeled
R3975 T13982 T13980 prep from,RNA
R3976 T13983 T13984 amod wild,type
R3977 T13984 T13986 compound type,cells
R3978 T13985 T13984 punct -,type
R3979 T13986 T13982 pobj cells,from
R3980 T13987 T13986 compound ES,cells
R3981 T13988 T13986 cc and,cells
R3982 T13989 T13990 npadvmod ESG1,null
R3983 T13990 T13992 amod null,cells
R3984 T13991 T13990 punct -,null
R3985 T13992 T13986 conj cells,cells
R3986 T13993 T13992 compound ES,cells
R3987 T13994 T13981 auxpass were,labeled
R3988 T13995 T13981 prep with,labeled
R3989 T13996 T13995 pobj Cy3,with
R3990 T13997 T13996 cc and,Cy3
R3991 T13998 T13996 conj Cy5,Cy3
R3992 T13999 T13981 punct ", ",labeled
R3993 T14000 T13981 advmod respectively,labeled
R3994 T14001 T13981 punct .,labeled
R3995 T14003 T14004 nsubjpass Samples,hybridized
R3996 T14005 T14004 auxpass were,hybridized
R3997 T14006 T14004 prep to,hybridized
R3998 T14007 T14008 nmod Agilent,Microarrays
R3999 T14008 T14006 pobj Microarrays,to
R4000 T14009 T14008 nmod Mouse,Microarrays
R4001 T14010 T14008 amod Developmental,Microarrays
R4002 T14011 T14004 punct .,hybridized
R4003 T14013 T14014 det The,averages
R4004 T14014 T14015 nsubjpass averages,shown
R4005 T14016 T14014 prep of,averages
R4006 T14017 T14018 nummod two,clones
R4007 T14018 T14016 pobj clones,of
R4008 T14019 T14018 amod independent,clones
R4009 T14020 T14015 auxpass are,shown
R4010 T14021 T14015 punct .,shown
R4011 T14023 T14024 meta B,analyses
R4012 T14025 T14023 punct ),B
R4013 T14026 T14027 compound Western,blot
R4014 T14027 T14024 compound blot,analyses
R4015 T14028 T14024 punct .,analyses
R4016 T14030 T14031 compound Cell,lysates
R4017 T14031 T14032 nsubjpass lysates,examined
R4018 T14033 T14031 prep from,lysates
R4019 T14034 T14035 nmod ESG1,cells
R4020 T14035 T14033 pobj cells,from
R4021 T14036 T14034 punct +,ESG1
R4022 T14037 T14034 punct /,ESG1
R4023 T14038 T14034 punct -,ESG1
R4024 T14039 T14034 cc and,ESG1
R4025 T14040 T14034 conj ESG1,ESG1
R4026 T14041 T14040 punct -,ESG1
R4027 T14042 T14040 punct /,ESG1
R4028 T14043 T14040 punct -,ESG1
R4029 T14044 T14032 auxpass were,examined
R4030 T14045 T14032 prep for,examined
R4031 T14046 T14045 pobj expression,for
R4032 T14047 T14046 prep of,expression
R4033 T14048 T14047 pobj ESG1,of
R4034 T14049 T14048 punct ", ",ESG1
R4035 T14050 T14048 conj Oct3,ESG1
R4036 T14051 T14050 punct /,Oct3
R4037 T14052 T14050 nummod 4,Oct3
R4038 T14053 T14050 cc and,Oct3
R4039 T14054 T14050 conj CDK4,Oct3
R4040 T14055 T14032 prep with,examined
R4041 T14056 T14055 pobj immunoblotting,with
R4042 T14057 T14032 punct .,examined
R828 T4123 T4124 compound ESG1,cDNA
R829 T4124 T4122 pobj cDNA,to
R830 T4125 T4119 punct ", ",produce
R831 T4126 T4119 nsubj they,produce
R832 T4127 T4119 aux could,produce
R833 T4128 T4119 neg not,produce
R834 T4129 T4130 amod functional,proteins
R835 T4130 T4119 dobj proteins,produce
R836 T4131 T4119 punct ", ",produce
R837 T4132 T4119 prep because,produce
R838 T4133 T4132 pcomp of,because
R839 T4134 T4135 amod critical,mutations
R840 T4135 T4132 pobj mutations,because
R841 T4136 T4119 punct .,produce
R842 T4138 T4139 det This,result
R843 T4139 T4140 nsubj result,suggests
R844 T4141 T4142 mark that,are
R845 T4142 T4140 ccomp are,suggests
R846 T4143 T4142 expl there,are
R847 T4144 T4145 det a,number
R848 T4145 T4142 attr number,are
R849 T4146 T4145 amod larger,number
R850 T4147 T4145 prep of,number
R851 T4148 T4149 amod intron-less,pseudogenes
R852 T4149 T4147 pobj pseudogenes,of
R853 T4150 T4151 mark than,anticipated
R854 T4151 T4145 advcl anticipated,number
R855 T4152 T4151 advmod previously,anticipated
R856 T4153 T4140 punct .,suggests
R857 T4155 T4156 nsubj Existence,is
R858 T4157 T4155 prep of,Existence
R859 T4158 T4159 amod multiple,retropseudogenes
R860 T4159 T4157 pobj retropseudogenes,of
R861 T4160 T4161 det a,hallmark
R1118 T4442 T4440 ccomp is,concluded
R1119 T4443 T4444 det this,sequence
R1120 T4444 T4442 nsubj sequence,is
R1121 T4445 T4446 det the,gene
R1122 T4446 T4442 attr gene,is
R1123 T4447 T4446 compound ESG1,gene
R1124 T4448 T4440 punct .,concluded
R1141 T4450 T4451 nsubj We,found
R1144 T4452 T4451 advmod also,found
R1145 T4453 T4454 mark that,contained
R1150 T4455 T4456 det the,clones
R1153 T4457 T4456 nummod two,clones
R1154 T4458 T4456 compound BAC,clones
R1375 T6488 T6485 punct ),geo
R1376 T6489 T6472 cc or,fusion
R1377 T6490 T6491 det the,gene
R1378 T6491 T6472 conj gene,fusion
R1379 T6492 T6493 npadvmod hygromycin,resistant
R1380 T6493 T6491 amod resistant,gene
R1381 T6494 T6495 punct (,HygR
R1382 T6495 T6491 parataxis HygR,gene
R1383 T6496 T6495 punct ),HygR
R1384 T6497 T6466 advcl using,replaced
R1385 T6498 T6499 nummod two,vectors
R1445 T6582 T6583 det t,an
R1446 T6588 T6587 compound ES,cells
R1447 T6589 T6586 punct ", ",introduced
R1448 T6590 T6586 nsubj we,introduced
R1449 T6591 T6592 det the,vector
R1450 T6592 T6586 dobj vector,introduced
R1451 T6593 T6594 compound β,geo
R1452 T6594 T6592 compound geo,vector
R1453 T6595 T6594 punct -,geo
R1454 T6596 T6586 prep into,introduced
R1455 T6597 T6598 npadvmod HygR,heterozygous
R1456 T6598 T6599 amod heterozygous,cells
R1457 T6599 T6596 pobj cells,into
R1458 T6600 T6599 compound ES,cells
R1459 T6601 T6586 punct .,introduced
R1460 T6603 T6604 prep Of,were
R1461 T6605 T6606 nummod 105,colonies
R1462 T6606 T6603 pobj colonies,Of
R1463 T6607 T6608 npadvmod G418,resistant
R1464 T6608 T6606 amod resistant,colonies
R1465 T6609 T6608 punct -,resistant
R1466 T6610 T6606 acl tested,colonies
R1467 T6611 T6604 punct ", ",were
R1468 T6612 T6604 nsubj 49,were
R1585 T6741 T6742 amod retinoic,treatment
R1586 T6742 T6731 conj treatment,removal
R1587 T6743 T6742 compound acid,treatment
R1588 T6744 T6745 punct (,shown
R1589 T6745 T6742 parataxis shown,treatment
R1590 T6746 T6745 neg not,shown
R1591 T6747 T6745 punct ),shown
R1592 T6748 T6727 punct .,differentiated
R1593 T6750 T6751 advmod When,transplanted
R1594 T6751 T6752 advcl transplanted,produced
R1595 T6753 T6751 prep into,transplanted
R1596 T6754 T6755 compound hind,flanks
R1597 T6755 T6753 pobj flanks,into
R1598 T6756 T6755 prep of,flanks
R1599 T6757 T6758 amod nude,mice
R1600 T6758 T6756 pobj mice,of
R1601 T6759 T6752 punct ", ",produced
R1602 T6760 T6761 det these,cells
R1603 T6761 T6752 nsubj cells,produced
R1604 T6762 T6752 dobj teratomas,produced
R1605 T6763 T6762 punct ", ",teratomas
R1606 T6764 T6762 appos tumors,teratomas
R1607 T6765 T6764 acl containing,tumors
R1608 T6766 T6765 dobj components,containing
R1609 T6767 T6766 prep of,components
R1610 T6768 T6769 det all,layers
R1611 T6769 T6767 pobj layers,of
R1612 T6770 T6769 nummod three,layers
R1613 T6771 T6769 compound germ,layers
R1637 T6804 T6803 nmod x,a
R1638 T6807 T6808 det the,profiles
R1639 T6808 T6806 dobj profiles,mined
R1640 T6809 T6808 compound gene,profiles
R1641 T6810 T6808 compound expression,profiles
R1642 T6811 T6808 prep of,profiles
R1643 T6812 T6813 nmod ESG1,cells
R1644 T6813 T6811 pobj cells,of
R1645 T6814 T6812 punct -,ESG1
R1646 T6815 T6812 punct /,ESG1
R1647 T6816 T6812 punct -,ESG1
R1648 T6817 T6813 compound ES,cells
R1649 T6818 T6806 advcl using,mined
R1650 T6819 T6820 npadvmod oligonucleotide,based
R1651 T6820 T6822 amod based,microarrays
R1652 T6821 T6820 punct -,based
R1783 T6964 T6963 cc or,directly
R1784 T6965 T6963 conj indirectly,directly
R1785 T6966 T6960 agent by,regulated
R1786 T6967 T6966 pobj ESG1,by
R1787 T6968 T6960 punct .,regulated
R1788 T6970 T6971 mark Since,contains
R1789 T6971 T6973 advcl contains,stabilize
R1790 T6972 T6971 nsubj ESG1,contains
R1791 T6974 T6975 det a,domain
R1792 T6975 T6971 dobj domain,contains
R1793 T6976 T6977 nmod KH,type
R1794 T6977 T6975 nmod type,domain
R1795 T6978 T6977 punct -,type
R1796 T6979 T6980 npadvmod RNA,binding
R1797 T6980 T6975 amod binding,domain
R1798 T6981 T6980 punct -,binding
R1799 T6982 T6973 punct ", ",stabilize
R1800 T6983 T6973 nsubj it,stabilize
R1801 T6984 T6973 aux may,stabilize
R1802 T6985 T6973 dobj mRNA,stabilize
R1803 T6986 T6985 prep of,mRNA
R1804 T6987 T6988 det these,genes
R1805 T6988 T6986 pobj genes,of
R1806 T6989 T6973 punct .,stabilize
R1807 T6991 T6992 advmod Further,studied
R1808 T6992 T6993 nsubjpass studied,required
R1809 T6994 T6993 auxpass are,required
R1810 T6995 T6996 aux to,clarify
R1811 T6996 T6993 advcl clarify,required
R1812 T6997 T6998 det this,possibility
R1813 T6998 T6996 dobj possibility,clarify
R1814 T6999 T6993 punct .,required
R1815 T7004 T7005 nmod gen,ra
R1816 T7006 T7005 nmod e,ra
R1817 T7012 T7011 punct -,ESG1
R1818 T7015 T7016 compound knockout,mice
R1819 T7017 T7014 punct ", ",injected
R1820 T7018 T7014 nsubj we,injected
R1821 T7019 T7020 nmod β,ESG1
R1822 T7020 T7024 nmod ESG1,clones
R1823 T7021 T7020 punct -,ESG1
R1824 T7022 T7020 nmod geo,ESG1
R1825 T7023 T7020 punct -,ESG1
R1826 T7024 T7014 dobj clones,injected
R1827 T7025 T7020 punct +,ESG1
R1828 T7026 T7020 punct /,ESG1
R1829 T7027 T7020 punct -,ESG1
R1830 T7028 T7024 compound ES,clones
R1831 T7029 T7024 compound cell,clones
R1832 T7030 T7014 prep into,injected
R1833 T7031 T7032 det the,blastocysts
R1834 T7032 T7030 pobj blastocysts,into
R1835 T7033 T7032 prep of,blastocysts
R1836 T7034 T7035 compound C57BL6,mice
R1837 T7035 T7033 pobj mice,of
R1838 T7036 T7014 punct .,injected
R1839 T7038 T7039 nsubj We,obtained
R1840 T7040 T7041 compound germline,transmission
R1841 T7041 T7039 dobj transmission,obtained
R1842 T7042 T7039 prep from,obtained
R1843 T7043 T7044 nummod three,clones
R1844 T7044 T7042 pobj clones,from
R1845 T7045 T7039 punct .,obtained
R1846 T7047 T7048 nsubj We,obtained
R1847 T7049 T7050 nmod ESG1,mice
R1848 T7050 T7048 dobj mice,obtained
R1849 T7051 T7049 punct -,ESG1
R1850 T7052 T7049 punct /,ESG1
R1851 T7053 T7049 punct -,ESG1
R1852 T7054 T7048 prep at,obtained
R1853 T7055 T7056 det the,ratios
R1854 T7056 T7054 pobj ratios,at
R1855 T7057 T7056 amod Mendelian,ratios
R1856 T7058 T7056 punct (,ratios