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PMC:1420271 / 18461-19277 JSONTXT

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craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T10360 23-27 PR:000006847 denotes Esg1
T10361 58-62 PR:000006847 denotes pH34
T10362 115-119 PR:000006847 denotes pH34
T10363 168-175 SO:0000112 denotes primers
T10364 196-200 PR:000006847 denotes pH34
T10365 216-227 SO:0000006 denotes PCR product
T10366 276-280 PR:000006847 denotes pH34
T10367 379-389 GO:0010467 denotes expression
T10368 390-396 SO:0000440 denotes vector
T10369 405-409 PR:000006847 denotes pH34
T10370 423-430 NCBITaxon:562 denotes E. coli
T10371 457-475 GO:0006412 denotes protein production
T10372 546-550 PR:000006847 denotes ESG1
T10373 573-594 CHEBI:44557 denotes nitrilotriacetic acid
T10374 595-602 CHEBI:2511 denotes agarose
T10375 663-667 CHEBI:16199 denotes urea
T10376 696-700 CHEBI:16199 denotes urea
T10377 764-771 NCBITaxon:9986 denotes rabbits
T10378 789-793 PR:000006847 denotes ESG1
T10379 805-815 GO:0042571 denotes antibodies

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T10384 4-19 SO_EXT:coding_sequence denotes coding sequence
T10385 23-27 PR_EXT:000006847 denotes Esg1
T10386 58-62 PR_EXT:000006847 denotes pH34
T10387 115-119 PR_EXT:000006847 denotes pH34
T10388 168-175 SO_EXT:0000112 denotes primers
T10389 196-200 PR_EXT:000006847 denotes pH34
T10390 216-227 SO_EXT:0000006 denotes PCR product
T10391 276-280 PR_EXT:000006847 denotes pH34
T10392 328-341 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T10393 379-389 GO:0010467 denotes expression
T10394 390-396 SO_EXT:0000440 denotes vector
T10395 405-409 PR_EXT:000006847 denotes pH34
T10396 423-430 NCBITaxon:562 denotes E. coli
T10397 445-456 GO_SO_EXT:sequence_rearrangement_process denotes recombinant
T10398 457-464 CHEBI_PR_EXT:protein denotes protein
T10399 457-475 GO:0006412 denotes protein production
T10400 529-538 CHEBI_SO_EXT:histidine denotes Histidine
T10401 539-545 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T10402 546-550 PR_EXT:000006847 denotes ESG1
T10403 570-572 CHEBI_EXT:nickel denotes Ni
T10404 573-594 CHEBI:44557 denotes nitrilotriacetic acid
T10405 595-602 CHEBI:2511 denotes agarose
T10406 618-628 GO_EXT:macromolecule_denaturation denotes denaturing
T10407 663-667 CHEBI:16199 denotes urea
T10408 696-700 CHEBI:16199 denotes urea
T10409 706-717 GO_SO_EXT:sequence_rearrangement_process denotes recombinant
T10410 718-726 CHEBI_PR_EXT:protein denotes proteins
T10411 764-771 NCBITaxon:9986 denotes rabbits
T10412 789-793 PR_EXT:000006847 denotes ESG1
T10413 805-815 GO:0042571 denotes antibodies

craft-sa-dev

Id Subject Object Predicate Lexical cue
T10420 0-3 DT denotes The
T10421 11-19 NN denotes sequence
T10422 4-10 VBG denotes coding
T10423 32-41 VBN denotes amplified
T10424 20-22 IN denotes of
T10425 23-27 NN denotes Esg1
T10426 28-31 VBD denotes was
T10427 42-44 IN denotes by
T10428 45-48 NN denotes PCR
T10429 49-53 IN denotes with
T10430 54-57 DT denotes the
T10431 168-175 NNS denotes primers
T10432 58-62 NN denotes pH34
T10433 66-67 NN denotes s
T10434 62-63 HYPH denotes -
T10435 63-65 NN denotes gw
T10436 65-66 HYPH denotes -
T10437 68-69 -LRB- denotes (
T10438 73-106 NN denotes AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
T10439 69-70 CD denotes 5
T10440 70-71 SYM denotes '
T10441 71-72 HYPH denotes -
T10442 106-107 HYPH denotes -
T10443 107-108 CD denotes 3
T10444 108-109 SYM denotes '
T10445 109-110 -RRB- denotes )
T10446 111-114 CC denotes and
T10447 115-119 NN denotes pH34
T10448 123-125 NN denotes as
T10449 119-120 HYPH denotes -
T10450 120-122 NN denotes gw
T10451 122-123 HYPH denotes -
T10452 126-127 -LRB- denotes (
T10453 131-163 NN denotes AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
T10454 127-128 CD denotes 5
T10455 128-129 SYM denotes '
T10456 129-130 HYPH denotes -
T10457 163-164 HYPH denotes -
T10458 164-165 CD denotes 3
T10459 165-166 SYM denotes '
T10460 166-167 -RRB- denotes )
T10461 175-176 . denotes .
T10462 176-269 sentence denotes To construct pDONR-pH34, the resulting PCR product was subcloned into pDONR201 (Invitrogen).
T10463 177-179 TO denotes To
T10464 180-189 VB denotes construct
T10465 232-241 VBN denotes subcloned
T10466 190-195 NN denotes pDONR
T10467 196-200 NN denotes pH34
T10468 195-196 HYPH denotes -
T10469 200-202 , denotes ,
T10470 202-205 DT denotes the
T10471 220-227 NN denotes product
T10472 206-215 VBG denotes resulting
T10473 216-219 NN denotes PCR
T10474 228-231 VBD denotes was
T10475 242-246 IN denotes into
T10476 247-255 NN denotes pDONR201
T10477 256-257 -LRB- denotes (
T10478 257-267 NNP denotes Invitrogen
T10479 267-268 -RRB- denotes )
T10480 268-269 . denotes .
T10481 269-342 sentence denotes pDONR-pH34 was interacted with pDEST17 (Invitrogen) by LR recombination.
T10482 270-275 NN denotes pDONR
T10483 276-280 NN denotes pH34
T10484 275-276 HYPH denotes -
T10485 285-295 VBN denotes interacted
T10486 281-284 VBD denotes was
T10487 296-300 IN denotes with
T10488 301-308 NN denotes pDEST17
T10489 309-310 -LRB- denotes (
T10490 310-320 NNP denotes Invitrogen
T10491 320-321 -RRB- denotes )
T10492 322-324 IN denotes by
T10493 325-327 NN denotes LR
T10494 328-341 NN denotes recombination
T10495 341-342 . denotes .
T10496 342-528 sentence denotes After introduction of the resulting expression vector pDEST17-pH34 into BL21-AI E. coli (Invitrogen), recombinant protein production was induced according to the manufacture's protocol.
T10497 343-348 IN denotes After
T10498 480-487 VBN denotes induced
T10499 349-361 NN denotes introduction
T10500 362-364 IN denotes of
T10501 365-368 DT denotes the
T10502 390-396 NN denotes vector
T10503 369-378 VBG denotes resulting
T10504 379-389 NN denotes expression
T10505 397-404 NN denotes pDEST17
T10506 405-409 NN denotes pH34
T10507 404-405 HYPH denotes -
T10508 410-414 IN denotes into
T10509 415-419 NN denotes BL21
T10510 420-422 NN denotes AI
T10511 419-420 HYPH denotes -
T10512 423-425 FW denotes E.
T10513 426-430 FW denotes coli
T10514 431-432 -LRB- denotes (
T10515 432-442 NNP denotes Invitrogen
T10516 442-443 -RRB- denotes )
T10517 443-445 , denotes ,
T10518 445-456 JJ denotes recombinant
T10519 457-464 NN denotes protein
T10520 465-475 NN denotes production
T10521 476-479 VBD denotes was
T10522 488-497 VBG denotes according
T10523 498-500 IN denotes to
T10524 501-504 DT denotes the
T10525 505-516 NN denotes manufacture
T10526 519-527 NN denotes protocol
T10527 516-518 POS denotes 's
T10528 527-528 . denotes .
T10529 528-668 sentence denotes Histidine-tagged ESG1 was purified using Ni-nitrilotriacetic acid agarose (Qiagen) under denaturing conditions in the presence of 8 M urea.
T10530 529-538 NN denotes Histidine
T10531 539-545 VBN denotes tagged
T10532 538-539 HYPH denotes -
T10533 546-550 NN denotes ESG1
T10534 555-563 VBN denotes purified
T10535 551-554 VBD denotes was
T10536 564-569 VBG denotes using
T10537 570-572 NN denotes Ni
T10538 595-602 NN denotes agarose
T10539 572-573 : denotes -
T10540 573-589 JJ denotes nitrilotriacetic
T10541 590-594 NN denotes acid
T10542 603-604 -LRB- denotes (
T10543 604-610 NNP denotes Qiagen
T10544 610-611 -RRB- denotes )
T10545 612-617 IN denotes under
T10546 618-628 NN denotes denaturing
T10547 629-639 NNS denotes conditions
T10548 640-642 IN denotes in
T10549 643-646 DT denotes the
T10550 647-655 NN denotes presence
T10551 656-658 IN denotes of
T10552 659-660 CD denotes 8
T10553 661-662 NN denotes M
T10554 663-667 NN denotes urea
T10555 667-668 . denotes .
T10556 668-816 sentence denotes After dialysis against 6 M urea, the recombinant proteins were injected into New Zealand White rabbits to generate anti-ESG1 polyclonal antibodies.
T10557 669-674 IN denotes After
T10558 732-740 VBN denotes injected
T10559 675-683 NN denotes dialysis
T10560 684-691 IN denotes against
T10561 692-693 CD denotes 6
T10562 694-695 NN denotes M
T10563 696-700 NN denotes urea
T10564 700-702 , denotes ,
T10565 702-705 DT denotes the
T10566 718-726 NN denotes proteins
T10567 706-717 JJ denotes recombinant
T10568 727-731 VBD denotes were
T10569 741-745 IN denotes into
T10570 746-749 NNP denotes New
T10571 750-757 NNP denotes Zealand
T10572 764-771 NNS denotes rabbits
T10573 758-763 JJ denotes White
T10574 772-774 TO denotes to
T10575 775-783 VB denotes generate
T10576 784-793 JJ denotes anti-ESG1
T10577 805-815 NNS denotes antibodies
T10578 794-804 JJ denotes polyclonal
T10579 815-816 . denotes .
R2895 T10420 T10421 det The,sequence
R2896 T10421 T10423 nsubjpass sequence,amplified
R2897 T10422 T10421 amod coding,sequence
R2898 T10424 T10421 prep of,sequence
R2899 T10425 T10424 pobj Esg1,of
R2900 T10426 T10423 auxpass was,amplified
R2901 T10427 T10423 agent by,amplified
R2902 T10428 T10427 pobj PCR,by
R2903 T10429 T10423 prep with,amplified
R2904 T10430 T10431 det the,primers
R2905 T10431 T10429 pobj primers,with
R2906 T10432 T10433 nmod pH34,s
R2907 T10433 T10431 nmod s,primers
R2908 T10434 T10433 punct -,s
R2909 T10435 T10433 nmod gw,s
R2910 T10436 T10433 punct -,s
R2911 T10437 T10438 punct (,AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2912 T10438 T10433 parataxis AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA,s
R2913 T10439 T10438 nummod 5,AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2914 T10440 T10439 punct ',5
R2915 T10441 T10438 punct -,AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2916 T10442 T10438 punct -,AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2917 T10443 T10438 nummod 3,AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2918 T10444 T10438 punct ',AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2919 T10445 T10438 punct ),AAAAAGCAGGCTGGATGATGGTGACCCTCGTGA
R2920 T10446 T10433 cc and,s
R2921 T10447 T10448 compound pH34,as
R2922 T10448 T10433 conj as,s
R2923 T10449 T10448 punct -,as
R2924 T10450 T10448 compound gw,as
R2925 T10451 T10448 punct -,as
R2926 T10452 T10453 punct (,AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2927 T10453 T10448 parataxis AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA,as
R2928 T10454 T10453 nummod 5,AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2929 T10455 T10454 punct ',5
R2930 T10456 T10453 punct -,AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2931 T10457 T10453 punct -,AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2932 T10458 T10453 nummod 3,AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2933 T10459 T10453 punct ',AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2934 T10460 T10453 punct ),AGAAAGCTGGGTCTGCATCCAGGTCGGAGACA
R2935 T10461 T10423 punct .,amplified
R2936 T10463 T10464 aux To,construct
R2937 T10464 T10465 advcl construct,subcloned
R2938 T10466 T10467 compound pDONR,pH34
R2939 T10467 T10464 dobj pH34,construct
R2940 T10468 T10467 punct -,pH34
R2941 T10469 T10465 punct ", ",subcloned
R2942 T10470 T10471 det the,product
R2943 T10471 T10465 nsubjpass product,subcloned
R2944 T10472 T10471 amod resulting,product
R2945 T10473 T10471 compound PCR,product
R2946 T10474 T10465 auxpass was,subcloned
R2947 T10475 T10465 prep into,subcloned
R2948 T10476 T10475 pobj pDONR201,into
R2949 T10477 T10478 punct (,Invitrogen
R2950 T10478 T10476 parataxis Invitrogen,pDONR201
R2951 T10479 T10478 punct ),Invitrogen
R2952 T10480 T10465 punct .,subcloned
R2953 T10482 T10483 compound pDONR,pH34
R2954 T10483 T10485 nsubjpass pH34,interacted
R2955 T10484 T10483 punct -,pH34
R2956 T10486 T10485 auxpass was,interacted
R2957 T10487 T10485 prep with,interacted
R2958 T10488 T10487 pobj pDEST17,with
R2959 T10489 T10490 punct (,Invitrogen
R2960 T10490 T10488 parataxis Invitrogen,pDEST17
R2961 T10491 T10490 punct ),Invitrogen
R2962 T10492 T10485 prep by,interacted
R2963 T10493 T10494 compound LR,recombination
R2964 T10494 T10492 pobj recombination,by
R2965 T10495 T10485 punct .,interacted
R2966 T10497 T10498 prep After,induced
R2967 T10499 T10497 pobj introduction,After
R2968 T10500 T10499 prep of,introduction
R2969 T10501 T10502 det the,vector
R2970 T10502 T10500 pobj vector,of
R2971 T10503 T10502 amod resulting,vector
R2972 T10504 T10502 compound expression,vector
R2973 T10505 T10506 compound pDEST17,pH34
R2974 T10506 T10502 appos pH34,vector
R2975 T10507 T10506 punct -,pH34
R2976 T10508 T10499 prep into,introduction
R2977 T10509 T10510 compound BL21,AI
R2978 T10510 T10508 pobj AI,into
R2979 T10511 T10510 punct -,AI
R2980 T10512 T10510 nmod E.,AI
R2981 T10513 T10510 nmod coli,AI
R2982 T10514 T10515 punct (,Invitrogen
R2983 T10515 T10499 parataxis Invitrogen,introduction
R2984 T10516 T10515 punct ),Invitrogen
R2985 T10517 T10498 punct ", ",induced
R2986 T10518 T10519 amod recombinant,protein
R2987 T10519 T10520 compound protein,production
R2988 T10520 T10498 nsubjpass production,induced
R2989 T10521 T10498 auxpass was,induced
R2990 T10522 T10498 prep according,induced
R2991 T10523 T10522 prep to,according
R2992 T10524 T10525 det the,manufacture
R2993 T10525 T10526 poss manufacture,protocol
R2994 T10526 T10523 pobj protocol,to
R2995 T10527 T10525 case 's,manufacture
R2996 T10528 T10498 punct .,induced
R2997 T10530 T10531 npadvmod Histidine,tagged
R2998 T10531 T10533 amod tagged,ESG1
R2999 T10532 T10531 punct -,tagged
R3000 T10533 T10534 nsubjpass ESG1,purified
R3001 T10535 T10534 auxpass was,purified
R3002 T10536 T10534 advcl using,purified
R3003 T10537 T10538 nmod Ni,agarose
R3004 T10538 T10536 dobj agarose,using
R3005 T10539 T10537 punct -,Ni
R3006 T10540 T10537 amod nitrilotriacetic,Ni
R3007 T10541 T10538 compound acid,agarose
R3008 T10542 T10543 punct (,Qiagen
R3009 T10543 T10538 parataxis Qiagen,agarose
R3010 T10544 T10543 punct ),Qiagen
R3011 T10545 T10536 prep under,using
R3012 T10546 T10547 compound denaturing,conditions
R3013 T10547 T10545 pobj conditions,under
R3014 T10548 T10536 prep in,using
R3015 T10549 T10550 det the,presence
R3016 T10550 T10548 pobj presence,in
R3017 T10551 T10550 prep of,presence
R3018 T10552 T10553 nummod 8,M
R3019 T10553 T10554 compound M,urea
R3020 T10554 T10551 pobj urea,of
R3021 T10555 T10534 punct .,purified
R3022 T10557 T10558 prep After,injected
R3023 T10559 T10557 pobj dialysis,After
R3024 T10560 T10559 prep against,dialysis
R3025 T10561 T10562 nummod 6,M
R3026 T10562 T10563 compound M,urea
R3027 T10563 T10560 pobj urea,against
R3028 T10564 T10558 punct ", ",injected
R3029 T10565 T10566 det the,proteins
R3030 T10566 T10558 nsubjpass proteins,injected
R3031 T10567 T10566 amod recombinant,proteins
R3032 T10568 T10558 auxpass were,injected
R3033 T10569 T10558 prep into,injected
R3034 T10570 T10571 nmod New,Zealand
R3035 T10571 T10572 nmod Zealand,rabbits
R3036 T10572 T10569 pobj rabbits,into
R3037 T10573 T10572 amod White,rabbits
R3038 T10574 T10575 aux to,generate
R3039 T10575 T10558 advcl generate,injected
R3040 T10576 T10577 amod anti-ESG1,antibodies
R3041 T10577 T10575 dobj antibodies,generate
R3042 T10578 T10577 amod polyclonal,antibodies
R3043 T10579 T10558 punct .,injected