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PMC:1420271 / 16394-17458 JSONTXT

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craft-ca-core-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id Subject Object Predicate Lexical cue
T9096 16-20 PR:000006847 denotes ESG1
T9097 21-38 SO:0001644 denotes targeting vectors
T9098 62-66 PR:000006847 denotes ESG1
T9099 67-72 SO:0000147 denotes exons
T9100 82-99 SO:0001644 denotes targeting vectors
T9101 121-125 SO:0000243 denotes IRES
T9102 132-140 SO:0005853 denotes cassette
T9103 152-156 SO:0000243 denotes IRES
T9104 162-170 SO:0005853 denotes cassette
T9105 174-182 SO:0000167 denotes promoter
T9106 229-231 SO:0000028 denotes bp
T9107 266-270 PR:000006847 denotes pH34
T9108 324-328 PR:000006847 denotes pH34
T9109 382-389 SO:0000112 denotes primers
T9110 408-410 SO:0000028 denotes bp
T9111 436-440 PR:000006847 denotes pH34
T9112 494-498 PR:000006847 denotes pH34
T9113 548-555 SO:0000112 denotes primers
T9114 561-565 SO:0000243 denotes IRES
T9115 575-579 SO:0000243 denotes IRES
T9116 585-594 SO:0005853 denotes cassettes
T9117 600-607 GO:0006266 denotes ligated
T9118 657-662 CHEBI:27026 denotes toxin
T9119 665-673 SO:0005853 denotes cassette
T9120 749-766 SO:0001644 denotes targeting vectors
T9121 806-814 CL:0002322 denotes ES cells
T9122 828-832 SO:0000704 denotes Gene
T9123 850-861 GO:0009294 denotes Transfected
T9124 897-901 CHEBI:42768 denotes G418
T9125 915-927 CHEBI:16976 denotes hygromycin B
T9126 943-950 SO:0001026 denotes Genomic
T9127 960-964 CHEBI:42768 denotes G418
T9128 969-981 CHEBI:16976 denotes hygromycin B

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id Subject Object Predicate Lexical cue
T9129 16-20 PR_EXT:000006847 denotes ESG1
T9130 21-38 SO_EXT:0001644 denotes targeting vectors
T9131 42-50 SO_EXT:sequence_substitution_process denotes replaced
T9132 62-66 PR_EXT:000006847 denotes ESG1
T9133 67-72 SO_EXT:0000147 denotes exons
T9134 82-99 SO_EXT:0001644 denotes targeting vectors
T9135 121-125 SO_EXT:0000243 denotes IRES
T9136 126-131 GO_EXT:beta_geo denotes β-geo
T9137 132-140 SO_EXT:0005853 denotes cassette
T9138 152-156 SO_EXT:0000243 denotes IRES
T9139 162-170 SO_EXT:0005853 denotes cassette
T9140 174-182 SO_EXT:0000167 denotes promoter
T9141 229-231 SO_EXT:0000028 denotes bp
T9142 266-270 PR_EXT:000006847 denotes pH34
T9143 324-328 PR_EXT:000006847 denotes pH34
T9144 382-389 SO_EXT:0000112 denotes primers
T9145 408-410 SO_EXT:0000028 denotes bp
T9146 436-440 PR_EXT:000006847 denotes pH34
T9147 494-498 PR_EXT:000006847 denotes pH34
T9148 548-555 SO_EXT:0000112 denotes primers
T9149 561-565 SO_EXT:0000243 denotes IRES
T9150 566-571 GO_EXT:beta_geo denotes β-geo
T9151 575-579 SO_EXT:0000243 denotes IRES
T9152 585-594 SO_EXT:0005853 denotes cassettes
T9153 600-607 GO:0006266 denotes ligated
T9154 657-662 CHEBI_EXT:27026 denotes toxin
T9155 665-673 SO_EXT:0005853 denotes cassette
T9156 685-695 SO_EXT:sequence_downstreamness denotes downstream
T9157 717-730 SO_EXT:sequence_linearization denotes linearization
T9158 749-766 SO_EXT:0001644 denotes targeting vectors
T9159 806-814 CL:0002322 denotes ES cells
T9160 809-814 CL_GO_EXT:cell denotes cells
T9161 828-832 SO_EXT:0000704 denotes Gene
T9162 850-861 GO:0009294 denotes Transfected
T9163 862-867 CL_GO_EXT:cell denotes cells
T9164 897-901 CHEBI:42768 denotes G418
T9165 915-927 CHEBI:16976 denotes hygromycin B
T9166 943-954 SO_EXT:genomic_DNA denotes Genomic DNA
T9167 951-954 CHEBI_SO_EXT:DNA denotes DNA
T9168 960-964 CHEBI:42768 denotes G418
T9169 969-981 CHEBI:16976 denotes hygromycin B
T9170 1029-1042 GO_SO_EXT:sequence_rearrangement_process denotes recombination

craft-sa-dev

Id Subject Object Predicate Lexical cue
T9171 0-12 NN denotes Construction
T9172 13-15 IN denotes of
T9173 16-20 NN denotes ESG1
T9174 31-38 NNS denotes vectors
T9175 21-30 NN denotes targeting
T9176 38-198 sentence denotes We replaced all of the ESG1 exons with two targeting vectors containing either an IRES-β-geo cassette [21] or an IRES-HygR cassette by promoter trap selection.
T9177 39-41 PRP denotes We
T9178 42-50 VBD denotes replaced
T9179 51-54 DT denotes all
T9180 55-57 IN denotes of
T9181 58-61 DT denotes the
T9182 67-72 NNS denotes exons
T9183 62-66 NN denotes ESG1
T9184 73-77 IN denotes with
T9185 78-81 CD denotes two
T9186 92-99 NNS denotes vectors
T9187 82-91 NN denotes targeting
T9188 100-110 VBG denotes containing
T9189 111-117 CC denotes either
T9190 132-140 NN denotes cassette
T9191 118-120 DT denotes an
T9192 121-125 NN denotes IRES
T9193 128-131 NN denotes geo
T9194 125-126 HYPH denotes -
T9195 126-127 NN denotes β
T9196 127-128 HYPH denotes -
T9197 141-142 -LRB- denotes [
T9198 142-144 CD denotes 21
T9199 144-145 -RRB- denotes ]
T9200 146-148 CC denotes or
T9201 149-151 DT denotes an
T9202 157-161 NN denotes HygR
T9203 152-156 NN denotes IRES
T9204 156-157 HYPH denotes -
T9205 162-170 NN denotes cassette
T9206 171-173 IN denotes by
T9207 174-182 NN denotes promoter
T9208 183-187 NN denotes trap
T9209 188-197 NN denotes selection
T9210 197-198 . denotes .
T9211 198-390 sentence denotes We amplified the 5' arm (1.8 kbp) using KOD plus (TOYOBO) with the pH34-targetpair5-U (5'-CCGCGGAAAGTCAAGAGATTGGGTGG-3') and pH34-targetpair5-L (5'-GCGGCCGCCTTTACGGGTCACGAGGGTCAC-3') primers.
T9212 199-201 PRP denotes We
T9213 202-211 VBD denotes amplified
T9214 212-215 DT denotes the
T9215 219-222 NN denotes arm
T9216 216-217 CD denotes 5
T9217 217-218 SYM denotes '
T9218 223-224 -LRB- denotes (
T9219 228-231 NN denotes kbp
T9220 224-227 CD denotes 1.8
T9221 231-232 -RRB- denotes )
T9222 233-238 VBG denotes using
T9223 239-242 NN denotes KOD
T9224 243-247 NN denotes plus
T9225 248-249 -LRB- denotes (
T9226 249-255 NN denotes TOYOBO
T9227 255-256 -RRB- denotes )
T9228 257-261 IN denotes with
T9229 262-265 DT denotes the
T9230 382-389 NNS denotes primers
T9231 266-270 NN denotes pH34
T9232 283-284 NN denotes U
T9233 270-271 HYPH denotes -
T9234 271-282 NN denotes targetpair5
T9235 282-283 HYPH denotes -
T9236 285-286 -LRB- denotes (
T9237 289-315 NN denotes CCGCGGAAAGTCAAGAGATTGGGTGG
T9238 286-287 CD denotes 5
T9239 287-288 SYM denotes '
T9240 288-289 HYPH denotes -
T9241 315-316 HYPH denotes -
T9242 316-317 CD denotes 3
T9243 317-318 SYM denotes '
T9244 318-319 -RRB- denotes )
T9245 320-323 CC denotes and
T9246 324-328 NN denotes pH34
T9247 341-342 NN denotes L
T9248 328-329 HYPH denotes -
T9249 329-340 NN denotes targetpair5
T9250 340-341 HYPH denotes -
T9251 343-344 -LRB- denotes (
T9252 347-377 NN denotes GCGGCCGCCTTTACGGGTCACGAGGGTCAC
T9253 344-345 CD denotes 5
T9254 345-346 SYM denotes '
T9255 346-347 HYPH denotes -
T9256 377-378 HYPH denotes -
T9257 378-379 CD denotes 3
T9258 379-380 SYM denotes '
T9259 380-381 -RRB- denotes )
T9260 389-390 . denotes .
T9261 390-556 sentence denotes The 3' arm (5.8 kbp) was amplified using the pH34-targetpair3-U (5'-TGTGGCCAGTGTTTGGTTCTGGCGGG-3') and pH34-targetpair3-L (5'-CTCGAGGACTCGCCATTCTAGCCAAG-3') primers.
T9262 391-394 DT denotes The
T9263 398-401 NN denotes arm
T9264 395-396 CD denotes 3
T9265 396-397 SYM denotes '
T9266 416-425 VBN denotes amplified
T9267 402-403 -LRB- denotes (
T9268 407-410 NN denotes kbp
T9269 403-406 CD denotes 5.8
T9270 410-411 -RRB- denotes )
T9271 412-415 VBD denotes was
T9272 426-431 VBG denotes using
T9273 432-435 DT denotes the
T9274 548-555 NNS denotes primers
T9275 436-440 NN denotes pH34
T9276 453-454 NN denotes U
T9277 440-441 HYPH denotes -
T9278 441-452 NN denotes targetpair3
T9279 452-453 HYPH denotes -
T9280 455-456 -LRB- denotes (
T9281 459-485 NN denotes TGTGGCCAGTGTTTGGTTCTGGCGGG
T9282 456-457 CD denotes 5
T9283 457-458 SYM denotes '
T9284 458-459 HYPH denotes -
T9285 485-486 HYPH denotes -
T9286 486-487 CD denotes 3
T9287 487-488 SYM denotes '
T9288 488-489 -RRB- denotes )
T9289 490-493 CC denotes and
T9290 494-498 NN denotes pH34
T9291 511-512 NN denotes L
T9292 498-499 HYPH denotes -
T9293 499-510 NN denotes targetpair3
T9294 510-511 HYPH denotes -
T9295 513-514 -LRB- denotes (
T9296 517-543 NN denotes CTCGAGGACTCGCCATTCTAGCCAAG
T9297 514-515 CD denotes 5
T9298 515-516 SYM denotes '
T9299 516-517 HYPH denotes -
T9300 543-544 HYPH denotes -
T9301 544-545 CD denotes 3
T9302 545-546 SYM denotes '
T9303 546-547 -RRB- denotes )
T9304 555-556 . denotes .
T9305 556-641 sentence denotes The IRES β-geo or IRES HygR cassettes were ligated in between the two PCR fragments.
T9306 557-560 DT denotes The
T9307 585-594 NNS denotes cassettes
T9308 561-565 NN denotes IRES
T9309 568-571 NN denotes geo
T9310 566-567 NN denotes β
T9311 567-568 HYPH denotes -
T9312 572-574 CC denotes or
T9313 575-579 NN denotes IRES
T9314 580-584 NN denotes HygR
T9315 600-607 VBN denotes ligated
T9316 595-599 VBD denotes were
T9317 608-610 IN denotes in
T9318 611-618 IN denotes between
T9319 619-622 DT denotes the
T9320 631-640 NNS denotes fragments
T9321 623-626 CD denotes two
T9322 627-630 NN denotes PCR
T9323 640-641 . denotes .
T9324 641-710 sentence denotes The diphtheria toxin A cassette was placed downstream of the 3' arm.
T9325 642-645 DT denotes The
T9326 665-673 NN denotes cassette
T9327 646-656 NN denotes diphtheria
T9328 663-664 NN denotes A
T9329 657-662 NN denotes toxin
T9330 678-684 VBN denotes placed
T9331 674-677 VBD denotes was
T9332 685-695 RB denotes downstream
T9333 696-698 IN denotes of
T9334 699-702 DT denotes the
T9335 706-709 NN denotes arm
T9336 703-704 CD denotes 3
T9337 704-705 SYM denotes '
T9338 709-710 . denotes .
T9339 710-849 sentence denotes After linearization with SacII, these targeting vectors were electroporated into 2.0 × 107 RF8 ES cells [22] using a Gene pulser (BIORAD).
T9340 711-716 IN denotes After
T9341 772-786 VBN denotes electroporated
T9342 717-730 NN denotes linearization
T9343 731-735 IN denotes with
T9344 736-741 NN denotes SacII
T9345 741-743 , denotes ,
T9346 743-748 DT denotes these
T9347 759-766 NNS denotes vectors
T9348 749-758 NN denotes targeting
T9349 767-771 VBD denotes were
T9350 787-791 IN denotes into
T9351 792-795 CD denotes 2.0
T9352 798-801 CD denotes 107
T9353 796-797 SYM denotes ×
T9354 809-814 NNS denotes cells
T9355 802-805 NN denotes RF8
T9356 806-808 NN denotes ES
T9357 815-816 -LRB- denotes [
T9358 816-818 CD denotes 22
T9359 818-819 -RRB- denotes ]
T9360 820-825 VBG denotes using
T9361 826-827 DT denotes a
T9362 833-839 NN denotes pulser
T9363 828-832 NN denotes Gene
T9364 840-841 -LRB- denotes (
T9365 841-847 NNP denotes BIORAD
T9366 847-848 -RRB- denotes )
T9367 848-849 . denotes .
T9368 849-942 sentence denotes Transfected cells were selected with 250 μg/mL G418 or 100 μg/mL hygromycin B, respectively.
T9369 850-861 VBN denotes Transfected
T9370 862-867 NNS denotes cells
T9371 873-881 VBN denotes selected
T9372 868-872 VBD denotes were
T9373 882-886 IN denotes with
T9374 887-890 CD denotes 250
T9375 891-893 NN denotes μg
T9376 897-901 NN denotes G418
T9377 893-894 SYM denotes /
T9378 894-896 NN denotes mL
T9379 902-904 CC denotes or
T9380 905-908 CD denotes 100
T9381 909-911 NN denotes μg
T9382 926-927 NN denotes B
T9383 911-912 SYM denotes /
T9384 912-914 NN denotes mL
T9385 915-925 NN denotes hygromycin
T9386 927-929 , denotes ,
T9387 929-941 RB denotes respectively
T9388 941-942 . denotes .
T9389 942-1064 sentence denotes Genomic DNA from G418- or hygromycin B-resistant colonies was screened for homologous recombination by Southern blotting.
T9390 943-950 JJ denotes Genomic
T9391 951-954 NN denotes DNA
T9392 1005-1013 VBN denotes screened
T9393 955-959 IN denotes from
T9394 960-964 NN denotes G418
T9395 982-991 JJ denotes resistant
T9396 964-965 HYPH denotes -
T9397 966-968 CC denotes or
T9398 969-979 NN denotes hygromycin
T9399 980-981 NN denotes B
T9400 981-982 HYPH denotes -
T9401 992-1000 NNS denotes colonies
T9402 1001-1004 VBD denotes was
T9403 1014-1017 IN denotes for
T9404 1018-1028 JJ denotes homologous
T9405 1029-1042 NN denotes recombination
T9406 1043-1045 IN denotes by
T9407 1046-1054 NNP denotes Southern
T9408 1055-1063 NN denotes blotting
T9409 1063-1064 . denotes .
R2461 T9172 T9171 prep of,Construction
R2462 T9173 T9174 compound ESG1,vectors
R2463 T9174 T9172 pobj vectors,of
R2464 T9175 T9174 compound targeting,vectors
R2465 T9177 T9178 nsubj We,replaced
R2466 T9179 T9178 dobj all,replaced
R2467 T9180 T9179 prep of,all
R2468 T9181 T9182 det the,exons
R2469 T9182 T9180 pobj exons,of
R2470 T9183 T9182 compound ESG1,exons
R2471 T9184 T9178 prep with,replaced
R2472 T9185 T9186 nummod two,vectors
R2473 T9186 T9184 pobj vectors,with
R2474 T9187 T9186 compound targeting,vectors
R2475 T9188 T9186 acl containing,vectors
R2476 T9189 T9190 preconj either,cassette
R2477 T9190 T9188 dobj cassette,containing
R2478 T9191 T9190 det an,cassette
R2479 T9192 T9193 compound IRES,geo
R2480 T9193 T9190 compound geo,cassette
R2481 T9194 T9193 punct -,geo
R2482 T9195 T9193 compound β,geo
R2483 T9196 T9193 punct -,geo
R2484 T9197 T9198 punct [,21
R2485 T9198 T9190 parataxis 21,cassette
R2486 T9199 T9198 punct ],21
R2487 T9200 T9190 cc or,cassette
R2488 T9201 T9202 det an,HygR
R2489 T9202 T9205 compound HygR,cassette
R2490 T9203 T9202 compound IRES,HygR
R2491 T9204 T9202 punct -,HygR
R2492 T9205 T9190 conj cassette,cassette
R2493 T9206 T9178 prep by,replaced
R2494 T9207 T9208 compound promoter,trap
R2495 T9208 T9209 compound trap,selection
R2496 T9209 T9206 pobj selection,by
R2497 T9210 T9178 punct .,replaced
R2498 T9212 T9213 nsubj We,amplified
R2499 T9214 T9215 det the,arm
R2500 T9215 T9213 dobj arm,amplified
R2501 T9216 T9215 nummod 5,arm
R2502 T9217 T9216 punct ',5
R2503 T9218 T9219 punct (,kbp
R2504 T9219 T9215 parataxis kbp,arm
R2505 T9220 T9219 nummod 1.8,kbp
R2506 T9221 T9219 punct ),kbp
R2507 T9222 T9213 advcl using,amplified
R2508 T9223 T9224 compound KOD,plus
R2509 T9224 T9222 dobj plus,using
R2510 T9225 T9226 punct (,TOYOBO
R2511 T9226 T9224 parataxis TOYOBO,plus
R2512 T9227 T9226 punct ),TOYOBO
R2513 T9228 T9224 prep with,plus
R2514 T9229 T9230 det the,primers
R2515 T9230 T9228 pobj primers,with
R2516 T9231 T9232 nmod pH34,U
R2517 T9232 T9230 nmod U,primers
R2518 T9233 T9232 punct -,U
R2519 T9234 T9232 nmod targetpair5,U
R2520 T9235 T9232 punct -,U
R2521 T9236 T9237 punct (,CCGCGGAAAGTCAAGAGATTGGGTGG
R2522 T9237 T9232 parataxis CCGCGGAAAGTCAAGAGATTGGGTGG,U
R2523 T9238 T9237 nummod 5,CCGCGGAAAGTCAAGAGATTGGGTGG
R2524 T9239 T9238 punct ',5
R2525 T9240 T9237 punct -,CCGCGGAAAGTCAAGAGATTGGGTGG
R2526 T9241 T9237 punct -,CCGCGGAAAGTCAAGAGATTGGGTGG
R2527 T9242 T9237 nummod 3,CCGCGGAAAGTCAAGAGATTGGGTGG
R2528 T9243 T9237 punct ',CCGCGGAAAGTCAAGAGATTGGGTGG
R2529 T9244 T9237 punct ),CCGCGGAAAGTCAAGAGATTGGGTGG
R2530 T9245 T9232 cc and,U
R2531 T9246 T9247 compound pH34,L
R2532 T9247 T9232 conj L,U
R2533 T9248 T9247 punct -,L
R2534 T9249 T9247 compound targetpair5,L
R2535 T9250 T9247 punct -,L
R2536 T9251 T9252 punct (,GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2537 T9252 T9247 parataxis GCGGCCGCCTTTACGGGTCACGAGGGTCAC,L
R2538 T9253 T9252 nummod 5,GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2539 T9254 T9253 punct ',5
R2540 T9255 T9252 punct -,GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2541 T9256 T9252 punct -,GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2542 T9257 T9252 nummod 3,GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2543 T9258 T9252 punct ',GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2544 T9259 T9252 punct ),GCGGCCGCCTTTACGGGTCACGAGGGTCAC
R2545 T9260 T9213 punct .,amplified
R2546 T9262 T9263 det The,arm
R2547 T9263 T9266 nsubjpass arm,amplified
R2548 T9264 T9263 nummod 3,arm
R2549 T9265 T9264 punct ',3
R2550 T9267 T9268 punct (,kbp
R2551 T9268 T9263 parataxis kbp,arm
R2552 T9269 T9268 nummod 5.8,kbp
R2553 T9270 T9268 punct ),kbp
R2554 T9271 T9266 auxpass was,amplified
R2555 T9272 T9266 advcl using,amplified
R2556 T9273 T9274 det the,primers
R2557 T9274 T9272 dobj primers,using
R2558 T9275 T9276 nmod pH34,U
R2559 T9276 T9274 nmod U,primers
R2560 T9277 T9276 punct -,U
R2561 T9278 T9276 nmod targetpair3,U
R2562 T9279 T9276 punct -,U
R2563 T9280 T9281 punct (,TGTGGCCAGTGTTTGGTTCTGGCGGG
R2564 T9281 T9276 parataxis TGTGGCCAGTGTTTGGTTCTGGCGGG,U
R2565 T9282 T9281 nummod 5,TGTGGCCAGTGTTTGGTTCTGGCGGG
R2566 T9283 T9282 punct ',5
R2567 T9284 T9281 punct -,TGTGGCCAGTGTTTGGTTCTGGCGGG
R2568 T9285 T9281 punct -,TGTGGCCAGTGTTTGGTTCTGGCGGG
R2569 T9286 T9281 nummod 3,TGTGGCCAGTGTTTGGTTCTGGCGGG
R2570 T9287 T9281 punct ',TGTGGCCAGTGTTTGGTTCTGGCGGG
R2571 T9288 T9281 punct ),TGTGGCCAGTGTTTGGTTCTGGCGGG
R2572 T9289 T9276 cc and,U
R2573 T9290 T9291 compound pH34,L
R2574 T9291 T9276 conj L,U
R2575 T9292 T9291 punct -,L
R2576 T9293 T9291 compound targetpair3,L
R2577 T9294 T9291 punct -,L
R2578 T9295 T9296 punct (,CTCGAGGACTCGCCATTCTAGCCAAG
R2579 T9296 T9291 parataxis CTCGAGGACTCGCCATTCTAGCCAAG,L
R2580 T9297 T9296 nummod 5,CTCGAGGACTCGCCATTCTAGCCAAG
R2581 T9298 T9297 punct ',5
R2582 T9299 T9296 punct -,CTCGAGGACTCGCCATTCTAGCCAAG
R2583 T9300 T9296 punct -,CTCGAGGACTCGCCATTCTAGCCAAG
R2584 T9301 T9296 nummod 3,CTCGAGGACTCGCCATTCTAGCCAAG
R2585 T9302 T9296 punct ',CTCGAGGACTCGCCATTCTAGCCAAG
R2586 T9303 T9296 punct ),CTCGAGGACTCGCCATTCTAGCCAAG
R2587 T9304 T9266 punct .,amplified
R2588 T9306 T9307 det The,cassettes
R2589 T9307 T9315 nsubjpass cassettes,ligated
R2590 T9308 T9309 nmod IRES,geo
R2591 T9309 T9307 nmod geo,cassettes
R2592 T9310 T9309 nmod β,geo
R2593 T9311 T9309 punct -,geo
R2594 T9312 T9309 cc or,geo
R2595 T9313 T9314 compound IRES,HygR
R2596 T9314 T9309 conj HygR,geo
R2597 T9316 T9315 auxpass were,ligated
R2598 T9317 T9315 prep in,ligated
R2599 T9318 T9317 prep between,in
R2600 T9319 T9320 det the,fragments
R2601 T9320 T9318 pobj fragments,between
R2602 T9321 T9320 nummod two,fragments
R2603 T9322 T9320 compound PCR,fragments
R2604 T9323 T9315 punct .,ligated
R2605 T9325 T9326 det The,cassette
R2606 T9326 T9330 nsubjpass cassette,placed
R2607 T9327 T9328 compound diphtheria,A
R2608 T9328 T9326 compound A,cassette
R2609 T9329 T9328 compound toxin,A
R2610 T9331 T9330 auxpass was,placed
R2611 T9332 T9330 advmod downstream,placed
R2612 T9333 T9332 prep of,downstream
R2613 T9334 T9335 det the,arm
R2614 T9335 T9333 pobj arm,of
R2615 T9336 T9335 nummod 3,arm
R2616 T9337 T9336 punct ',3
R2617 T9338 T9330 punct .,placed
R2618 T9340 T9341 prep After,electroporated
R2619 T9342 T9340 pobj linearization,After
R2620 T9343 T9342 prep with,linearization
R2621 T9344 T9343 pobj SacII,with
R2622 T9345 T9341 punct ", ",electroporated
R2623 T9346 T9347 det these,vectors
R2624 T9347 T9341 nsubjpass vectors,electroporated
R2625 T9348 T9347 compound targeting,vectors
R2626 T9349 T9341 auxpass were,electroporated
R2627 T9350 T9341 prep into,electroporated
R2628 T9351 T9352 quantmod 2.0,107
R2629 T9352 T9354 nummod 107,cells
R2630 T9353 T9352 punct ×,107
R2631 T9354 T9350 pobj cells,into
R2632 T9355 T9354 compound RF8,cells
R2633 T9356 T9354 compound ES,cells
R2634 T9357 T9358 punct [,22
R2635 T9358 T9354 parataxis 22,cells
R2636 T9359 T9358 punct ],22
R2637 T9360 T9341 advcl using,electroporated
R2638 T9361 T9362 det a,pulser
R2639 T9362 T9360 dobj pulser,using
R2640 T9363 T9362 compound Gene,pulser
R2641 T9364 T9365 punct (,BIORAD
R2642 T9365 T9362 parataxis BIORAD,pulser
R2643 T9366 T9365 punct ),BIORAD
R2644 T9367 T9341 punct .,electroporated
R2645 T9369 T9370 amod Transfected,cells
R2646 T9370 T9371 nsubjpass cells,selected
R2647 T9372 T9371 auxpass were,selected
R2648 T9373 T9371 prep with,selected
R2649 T9374 T9375 nummod 250,μg
R2650 T9375 T9376 nmod μg,G418
R2651 T9376 T9373 pobj G418,with
R2652 T9377 T9378 punct /,mL
R2653 T9378 T9375 prep mL,μg
R2654 T9379 T9376 cc or,G418
R2655 T9380 T9381 nummod 100,μg
R2656 T9381 T9382 nmod μg,B
R2657 T9382 T9376 conj B,G418
R2658 T9383 T9384 punct /,mL
R2659 T9384 T9381 prep mL,μg
R2660 T9385 T9382 compound hygromycin,B
R2661 T9386 T9371 punct ", ",selected
R2662 T9387 T9371 advmod respectively,selected
R2663 T9388 T9371 punct .,selected
R2664 T9390 T9391 amod Genomic,DNA
R2665 T9391 T9392 nsubjpass DNA,screened
R2666 T9393 T9391 prep from,DNA
R2667 T9394 T9395 npadvmod G418,resistant
R2668 T9395 T9401 amod resistant,colonies
R2669 T9396 T9394 punct -,G418
R2670 T9397 T9394 cc or,G418
R2671 T9398 T9399 compound hygromycin,B
R2672 T9399 T9394 conj B,G418
R2673 T9400 T9395 punct -,resistant
R2674 T9401 T9393 pobj colonies,from
R2675 T9402 T9392 auxpass was,screened
R2676 T9403 T9392 prep for,screened
R2677 T9404 T9405 amod homologous,recombination
R2678 T9405 T9403 pobj recombination,for
R2679 T9406 T9392 agent by,screened
R2680 T9407 T9408 compound Southern,blotting
R2681 T9408 T9406 pobj blotting,by
R2682 T9409 T9392 punct .,screened